LOCUS       DAORPG010000001        13640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_160_length_13640_cov_3.626205, whole genome shotgun sequence.
ACCESSION   DAORPG010000001 DAORPG010000000
VERSION     DAORPG010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 13640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13640
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160_length_13640_cov_3.626205"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PLX85_00005"
     CDS             <1..340
                     /locus_tag="PLX85_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61588.1"
                     /translation="DRGGDYNYECGLGLVGSSRDQTASHDSGPQRNQMFAECLIVDGF
                     QRVDLGLGLAYLQNVDWYNGSNLNFSLLARWRITDRFWLTYRHWSNSGTVMPNRGRDR
                     TMGGFVLVLP"
     gene            346..549
                     /locus_tag="PLX85_00010"
     CDS             346..549
                     /locus_tag="PLX85_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61589.1"
                     /translation="MARDIGKHEAQIEALQQDVHAMREDLTEIKALLNQTKGGMRVLI
                     GVGSIGGAVGAALVKFIAMLKGA"
     gene            546..1043
                     /locus_tag="PLX85_00015"
     CDS             546..1043
                     /locus_tag="PLX85_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HRC61590.1"
                     /translation="MSDEPVWLRIARADIGLREIKGAPTAPKIAAWLQQLGAWWRDDE
                     QPWCGTCLAAWMKGAGLSIPKAWYRALAWAQWGAEVAPTPGAVVVFQRPGGGHVGLLV
                     GRTNTGALLVLGGNQSDAVCILAFNPARVVAIRWPIERLADLGAAPADLPLLAATGPL
                     SRNEA"
     gene            1040..1420
                     /locus_tag="PLX85_00020"
     CDS             1040..1420
                     /locus_tag="PLX85_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61591.1"
                     /translation="MIETLTAWLAFLGWSADNVLAIVNGAVAATVAGWIAKYPLRVYC
                     DAHGIPLPTFKWLVRIVTGVAAIVATELSWPVRGRAAWFAGLLAWVAITLIYRYSAPL
                     LTKFFPWISTDALAKPDDSDEAGA"
     gene            1592..2056
                     /locus_tag="PLX85_00025"
     CDS             1592..2056
                     /locus_tag="PLX85_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61592.1"
                     /translation="MNALELRLVGYGLAIAAALGLYTYVHELRSQAARVAPLEALVAG
                     YRANEQLRKEQDHAITKDVAATAAAAAVSALGPVRLCRPAAVPTPAAASPAGPSAGAG
                     AGALPSGVEAGPDIGPQLERTGDEADALIVTARACQAYVRSLPAAYRLPGAP"
     gene            complement(2067..2348)
                     /locus_tag="PLX85_00030"
     CDS             complement(2067..2348)
                     /locus_tag="PLX85_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61593.1"
                     /translation="MGVAGSATLRSHWHLSGHEVTEDRFRQCLARAEQCCDFQPASHV
                     SKNFTRDDGRSYAVPEWVLAITTPGDILTLEMADEPKWYVNGNRVIGPR"
     gene            complement(2682..2912)
                     /locus_tag="PLX85_00035"
     CDS             complement(2682..2912)
                     /locus_tag="PLX85_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61594.1"
                     /translation="MSGTVEFWRGTQVAEFEDRQWVPLESYVRLRDALDEIAKQADSE
                     YSQEHLILVWIARKALQTDVARAAGQTPETRA"
     gene            complement(2909..3136)
                     /locus_tag="PLX85_00040"
     CDS             complement(2909..3136)
                     /locus_tag="PLX85_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61595.1"
                     /translation="MKEFRADGIYSTDDFIVVQVERDGKVCVEINEPWAGDTQTGFGR
                     HCSIDLSAEDALELRDFLNDRLPSTAPEVPK"
     gene            complement(3981..4967)
                     /locus_tag="PLX85_00045"
     CDS             complement(3981..4967)
                     /locus_tag="PLX85_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRC61596.1"
                     /translation="MARPRTINKHLPKYVTVIHGAYWFRPPKAKAVRLAALGDEPGMY
                     RALADQVAAVPSGPLTTLADCFDRYEREIVPTLAPRTQKDYRRHLSILRRWCGDVRPD
                     DLKPRDVGRFLDVDKGKIHRNKQIGVLSAVYTKMVGRWYVAERNPCVGVERNRKGKRD
                     RYVTDAEFAAVYAVVPPRLQVAMDLALLTGQRQGDLLALQWDAVDEAGVHFRQGKTGK
                     RLVVEISPALEAVLLRARQLVPQLPRRYVLRTRKGQRYSSEGFRAIWQRRMAKVLRSG
                     ALKERFTFHDLRAKSVSDSKTLDEAYERAGHASMSMTRGVYDRAERRVKPLR"
     gene            complement(4969..5169)
                     /locus_tag="PLX85_00050"
     CDS             complement(4969..5169)
                     /locus_tag="PLX85_00050"
                     /inference="COORDINATES: protein motif:HMM:NF025352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HRC61597.1"
                     /translation="MSLWLNDDELYELTGYRQRDRQRQALVNMGVKFRTRPADGCPMV
                     HRAQFEAEPQGRGKLDWKAVGA"
     gene            complement(5154..6281)
                     /locus_tag="PLX85_00055"
     CDS             complement(5154..6281)
                     /locus_tag="PLX85_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
                     /protein_id="HRC61598.1"
                     /translation="MSFRDLHAAWRAARRGKKPSVDQIAFDAQWMDRLLELQERLNTL
                     TWSPSPCTCFIASQPKAREIHAPAFPDRVVHHYLVPRLEAIYEPRFVHDSYSNRRGKG
                     THAAVDRLQAFVRQVQSGQSGGWYLQLDIRNCFNRIHRPTLYRMLKREMGRHDLPLPI
                     RKATHALLARSCAETGVRYACTPEQRAAVPLHKRLEGAPPGCGIAIGNLSSQFFANVY
                     LDALDQFVKHELKTTRYLRYVDDFVLVHSDRDQLVAWRERIERFLANELRLELKPSPP
                     LRRLTEGIDFLGYVVFPTHRLVRRRVIAHARAKLAAWEREHVRPSGLRASNAALEHLR
                     SIWASYLGHFRRASSYRVRERIFQRFAWLPYSLVRTRCRSG"
     gene            complement(6455..6889)
                     /locus_tag="PLX85_00060"
     CDS             complement(6455..6889)
                     /locus_tag="PLX85_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRC61599.1"
                     /translation="MRDGLPPIVKLSTRLLVDLERAVRRFPRYHKYAVGADLRAQAMA
                     VARCAQRAWRDRAHQLERVDQLGIAIDELKLTMQLGKEIEVFQSFGEFEALARVASDL
                     GRQCGGWRKQLHLKSQNGPATPVPAQRAQRLSSRAAPAGVHP"
     gene            complement(6907..7470)
                     /locus_tag="PLX85_00065"
     CDS             complement(6907..7470)
                     /locus_tag="PLX85_00065"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HRC61600.1"
                     /translation="MNSLSQELKQVVKRRLDQAPAAEVLRIVSTLCADDGVAPAAPVR
                     LAGPRFTKISDDGRAMAMDSGQWVAVHDAVTDLVWTRAPLEGRHQWKAAHQAAESCAL
                     CGQAGWRLPTVTELLTLVDYSRADPAIDTSFFTEAGNWYWTSTPCAPSPSVSAWFVYF
                     DNGYSFWRHQNYEGLVRAVRAGQSLGL"
     gene            complement(7501..7911)
                     /locus_tag="PLX85_00070"
     CDS             complement(7501..7911)
                     /locus_tag="PLX85_00070"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HRC61601.1"
                     /translation="MSRFTKISVDGQDLPADAKNWVAVRDETTKLVWSAGEAKKPMRW
                     KKAKAYAEKLDLCGWKWRLPTVEELFLLADRSRTAPAIDIAFFPECQSKWYWSGTVAA
                     PSPSDSAWCVYFYDGDSSWTLQYDEGLVRAVRAG"
     gene            complement(7925..8485)
                     /locus_tag="PLX85_00075"
     CDS             complement(7925..8485)
                     /locus_tag="PLX85_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61602.1"
                     /translation="MNLMLEARGLRPAFELAAQRPHGDRLRYLGGCRCFKCRRSNSDY
                     ERHRQAARAAGDWNGIVDAAAARAHLIKLSRIGIGRRAVAAASDVAETVISEIRSGRK
                     ARIRARTERRILAVTKTCASDHALVSARRTWKLLNELIAEGFTRTDLAARLGSSARHP
                     ALQIKHDFVLARTAHRVEVLHRQLTT"
     gene            complement(8490..8786)
                     /locus_tag="PLX85_00080"
     CDS             complement(8490..8786)
                     /locus_tag="PLX85_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61603.1"
                     /translation="MSSDFLVTSMASLRSMLRAGAVLNVKQMAWSGAWAGISVTHAGK
                     TTPVHTSLWLRLYRRGGLQISDDRVSAGGERTFCVRLAGPSSAPPRSRAGSGSR"
     gene            complement(8796..9065)
                     /locus_tag="PLX85_00085"
     CDS             complement(8796..9065)
                     /locus_tag="PLX85_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61604.1"
                     /translation="MNAVAAAIEPLPEAEREAAIDDALELLSEQITRRNAARMAQWSV
                     WRLAQHAGALRARAIVTSDPTMPNGLRIEFETLQVPAAFRSEKPA"
     gene            complement(9062..9277)
                     /locus_tag="PLX85_00090"
     CDS             complement(9062..9277)
                     /locus_tag="PLX85_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61605.1"
                     /translation="MTLDHAIDLAKDAAASRRENVFVVLDGPSFHVGTKATLESLLKD
                     HPVYASVWPDGRVVTVADQRAMRQVAA"
     gene            9384..9776
                     /locus_tag="PLX85_00095"
     CDS             9384..9776
                     /locus_tag="PLX85_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61606.1"
                     /translation="MESAPQRVIVTDVDIKFGSMVTLLIKLAFAALPAAIFVGAVGFI
                     AVMLAGGIGGSVISALKGGGTPASIEAPTPRDPKIDESLIAARRLLAAGDRENAEEIL
                     RRAMLTGAGSIEQRQDLIETLRRAQAAN"
     gene            10274..10510
                     /locus_tag="PLX85_00100"
     CDS             10274..10510
                     /locus_tag="PLX85_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HRC61607.1"
                     /translation="MTLKEFLQTLSLADRSVFAQSVGSSAGHLRNISYGYRPCSEGLA
                     IEIEKLSAGIVRCEDLCPDVDWSYLRNSQRELAP"
     gene            10507..10929
                     /locus_tag="PLX85_00105"
     CDS             10507..10929
                     /locus_tag="PLX85_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61608.1"
                     /translation="MTRSTGIHDSGNAMQAVLFHDSIADAIGTAIAAIGGPKKAGGAL
                     WPADPVARSEARVRSCLNAERAEKFSPDEVMQLARLARAAGDHSIMNYLAAELGYEIR
                     PLDPQDQMAALQQQYIDAARSIRSIAERIERIQVRAVG"
     gene            complement(10986..11243)
                     /locus_tag="PLX85_00110"
     CDS             complement(10986..11243)
                     /locus_tag="PLX85_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61609.1"
                     /translation="MGSMEAQVAQTLDAQELTVDCPKCSHQITKTLRSLRTHHEMDCP
                     GCRATITLDTDGLNRGYQEALQSAREIDRKLSIPIKLTLKF"
     gene            12215..12493
                     /locus_tag="PLX85_00115"
     CDS             12215..12493
                     /locus_tag="PLX85_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61610.1"
                     /translation="MALLLEWFAARVKKTLTWELRRASVGKDAYSLETFLCAEFPGGP
                     YTVVRSAGFADDAWYELFAAEKSVGAWLTSSREGCALAEWHAAGAPAL"
     gene            12490..13197
                     /locus_tag="PLX85_00120"
     CDS             12490..13197
                     /locus_tag="PLX85_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61611.1"
                     /translation="MSDPRQVVIDALRSGPKNYQSLRMATHLEPGAMDAALTALKRDG
                     LVKIDMGFWELVAETPQPRKTLEVTISGPVASGDSLLEVLHAAADRDVMVTAPTDPPP
                     TSEDHDPMASTKHCKRCDKRKPVSAFGKNATRSDGLQTYCRPCMNEATSKRKDAPARA
                     KKVAAPVVSTNAEAESPEPENREIHCDLFFVTHRTVSAQWSGEYVAIDVRQDGVSERV
                     VITRRELRAIAAQDLNE"
     gene            13206..13586
                     /locus_tag="PLX85_00125"
     CDS             13206..13586
                     /locus_tag="PLX85_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61612.1"
                     /translation="MLQLDAFGPPPTPRRREARLKRDLGMIRAELSQERASHDWNLQA
                     AMYLREYARRIAQGQPFKIEDAREASASRVPVPKNQKSWGPAVMFAARQKWIKRAEGP
                     DGRPMYDRARSSNLSPKPLWVAGE"
BASE COUNT         2194 a         4655 c         4470 g         2321 t
ORIGIN      
        1 agaccgcggc ggcgactaca actacgagtg cggcctcggc ctggtcggct ccagtcgcga
       61 ccagaccgcg agccacgaca gtggcccgca gcgcaatcag atgttcgcgg agtgcctgat
      121 cgtggacggg ttccagcggg tcgatctcgg cctgggtctc gcctacctgc agaacgtcga
      181 ttggtacaac ggctcgaacc tcaacttctc gctgctcgcg cggtggcgca tcaccgaccg
      241 gttctggctg acctaccgcc actggtccaa ctccgggacc gtcatgccga accgcggccg
      301 tgatcgtacg atggggggtt tcgtcctggt gctgccgtga cggatgtggc gcgcgacatc
      361 ggtaagcacg aggcgcagat cgaggcgctc cagcaggacg tgcacgcgat gcgcgaggac
      421 ctgaccgaga tcaaggccct gctcaaccag accaagggcg gaatgcgcgt actgatcggc
      481 gtcggctcga tcggcggcgc ggtgggagcg gcgctcgtca agttcatcgc catgctgaag
      541 ggcgcatgag cgacgagccc gtctggctgc gcatcgcccg ggccgacatc gggctgcgcg
      601 agatcaaggg ggcacccacc gctcccaaga ttgccgcctg gctgcagcag ctcggtgcct
      661 ggtggcggga tgacgaacag ccatggtgcg gcacctgtct cgccgcatgg atgaagggcg
      721 ccggtctctc gatcccgaag gcctggtatc gggcgctcgc ctgggcgcag tggggcgcgg
      781 aggtcgcgcc gacacccggt gccgtggtcg tattccagcg gcctggcggt ggccacgtcg
      841 gcctgctcgt cggtcggacg aataccggtg ccctgctggt actgggcggc aatcagtccg
      901 atgcggtctg catcctggcg ttcaaccccg cgcgcgtcgt cgccatccgc tggcccattg
      961 agcgcctcgc ggatctcggc gccgctcctg ccgacctgcc gctgctcgcc gcaacgggac
     1021 cgctctcgag gaacgaagca tgatcgagac cctcaccgcc tggctggcct tcctcggctg
     1081 gtccgccgac aacgtgctcg ccatcgtgaa cggcgccgtg gcggcaaccg tcgcaggctg
     1141 gatcgcgaag taccccctgc gcgtctactg cgatgcgcac ggcatcccgc tgcccacctt
     1201 caagtggctg gtgcgcatcg tcacgggcgt cgccgccatc gtcgcgaccg aactgtcctg
     1261 gcccgtgcgc ggccgcgccg cctggttcgc cgggctcctg gcctgggtcg cgatcacgct
     1321 gatctaccgc tacagcgcac cgttgctgac gaagttcttc ccatggatca gcaccgacgc
     1381 gctcgcgaag ccggacgact ccgatgaagc cggcgcctga cctctgccaa cgctgccgct
     1441 cttgctgccc atggaacggg aaggactggg gctgtagtcg cgctgagtat cacgggcccc
     1501 tcgcgggcac cgagccgccc tgcggagggc gactgttcgc ggagcctgcg cctttccgct
     1561 ggccgccggc cccggccgaa ggagccagcc cgtgaacgcg ctggagctgc gcctcgtggg
     1621 ctacgggctg gcgatcgccg cggcccttgg gctctacacc tacgtccacg agctccgctc
     1681 acaggccgcg agagtcgctc cgctcgaggc tttggtggcc ggctatcggg cgaatgaaca
     1741 actgcgcaag gagcaggacc atgccatcac gaaagacgtc gcggccacgg ctgctgctgc
     1801 tgctgtctct gccctcggtc ctgtccgcct gtgccgccca gccgcagtgc cgacccctgc
     1861 cgcagccagc cccgcaggtc ctagcgccgg tgccggagcc ggggcacttc cgtcgggagt
     1921 tgaagcgggt cctgacatcg gacctcagct cgagcgaacc ggcgatgaag ccgacgccct
     1981 gatcgtcacc gcgcgcgcgt gccaggccta cgtgcgctcg ctgcccgccg cctatcgcct
     2041 gccaggcgct ccctgacgat cgccggtcac cgtgggccga tgacccggtt cccattcacg
     2101 taccatttcg gctcgtcggc catctcgagg gtgaggatgt ctcccggcgt ggtgatggcc
     2161 agcacccact ccggcaccgc ataggatcgg ccatcgtccc gggtgaaatt ctttgatacg
     2221 tggctcgcgg gctgaaaatc gcagcattgc tccgccctgg cgagacactg acggaaccga
     2281 tcctccgtta cctcatgccc gctcaggtgc cagtgcgagc gcagcgttgc cgatccggca
     2341 acgcccattt ccccgtcgat gatctggcat gcgctgctcc accgatcgcc cggagtgaca
     2401 tcggaccaac gcgggtggcc cggatcgaag acccaccact tgcactcaac gcatgttctg
     2461 ctcatgtgtc accacgttgg ttggcccatc gaggggagcc cagtgagtca cctgctccga
     2521 aaggaacagg tactcgtcgt gtgtagtcca gactccggcg ctcacaaccc actccagtct
     2581 cctggcggac cagtaggcta ccgcatggct tccatcaatc atgtatacca gcacgtcctc
     2641 gtcctgaggg gctggcccga cggacacatc atgccattca atcacgcgcg agtttctggc
     2701 gtctgaccag cggctcgcgc gacgtcggtt tgcagcgcct tgcgagcgat ccagaccagt
     2761 atcaggtgct cctgggaata ctccgagtca gcctgtttgg cgatctcgtc gagcgcatcc
     2821 ctcaatcgca catagctttc cagtggcacc cattgccgat cctcgaactc tgccacctgc
     2881 gtcccgcgcc agaattcaac cgttccgctc acttcggcac ctcgggtgcc gttgacggta
     2941 gcctatcgtt caggaagtcg cgcagctcga gcgcgtcttc ggcggaaagg tcgatgctgc
     3001 aatgcctgcc gaaaccggtt tgggtgtcac cagcccacgg ctcgttaatc tcgacgcata
     3061 ccttgccgtc gcgctcaact tgaacgacga tgaagtcgtc ggtgctgtaa atcccatccg
     3121 cgcgaaactc tttcacttcc gttcctcggg tgcgctgcga tcggtgctat cgaaaagggc
     3181 ggcagccccg gccgcgaggg gttcccgtgt gaggcgcttg cgccacagac agaggcccag
     3241 agccgaagct ccttccgttg gcactgtcgt gcgggtgcgg tctcacgggc ggggctgcca
     3301 taggatcaat tcgctggctt cgtcacgcgc gcatggatcg cttccgcagc ggaccaccac
     3361 gcattcctca cagcgccagg cagcgcatcc cacgccggca ggggatcgcc ggttactgcg
     3421 gagcgtccgc cggtcgtgcg gctgtacgct tcgtatcccg cgcgggccaa cggccagatc
     3481 gcttcttgct caggtgtcag ttccatcact cgtctccttt ggttgaaata ctatctggtc
     3541 cgctacccac gtctcgctct gcctgaactt ctcgactcca cgagtcgaac ggctcctgca
     3601 gcccttccgc cggccagaac tcgcagcgct cgcggcgcgg gcacaggatc gccatggcgc
     3661 cggtggtgat tgctgcgccg caggtgtcac aggtcgggca ctcgttcatg gagacgcgtc
     3721 cccgttcgca gtgcccccgc ctgttgcgaa cctcgccaca aaccccgcga aaagccgggg
     3781 taaagcctcc ctgactcgat cgaatgcgca acagttggcg cggtcttcct gctcgcggcg
     3841 acaacggctt aaacggctgt aatcgctcgt ccttttaacc agttggtcgc gcgttcgagt
     3901 cgcgcagggc ccagttattt tcaacgactt ggacactgta cgagtctcca gcatggtcgc
     3961 aaattcggtg ggggtgtttc tcatcgcagc ggtttgaccc ggcgctcagc ccggtcgtac
     4021 acacctcggg tcatgctcat cgacgcgtgt cccgcgcgct cataagcctc gtcgagcgtc
     4081 ttgctgtcgc tcaccgactt cgcccgcagg tcgtggaagg tgaagcgctc cttgagagca
     4141 ccagacctca gcaccttcgc catgcggcgc tgccagatcg cgcggaaccc ttccgacgag
     4201 taccgctggc ccttgcgcgt gcgcagcacg tagcggcggg gtagctgggg caccagttgc
     4261 cgggcacgca gcagcacggc ctcgagggcg gggctgatct cgacgaccag gcgcttaccc
     4321 gtcttcccct ggcggaagtg cacgccggcc tcgtccaccg cgtcccattg cagcgcgagc
     4381 aaatcgccct gccgctggcc ggtgagcagc gcaaggtcca tcgccacctg caggcggggc
     4441 ggcaccacgg cgtacacggc ggcgaactcc gcatccgtca cgtagcgatc ccgcttgccc
     4501 ttcctgttgc gctccacccc gacgcagggg ttgcgctcgg ccacgtacca gcggccgacc
     4561 atcttggtgt agacggccga cagcacgccg atctgcttgt tgcggtggat cttgcccttg
     4621 tccacgtcga gaaagcggcc gacgtcgcgc ggcttcaggt catccgggcg cacatcgccg
     4681 caccagcggc gcaggatgga taggtgccgg cggtagtctt tctgcgtgcg tggcgcgagc
     4741 gtcggcacga tctcgcgttc gtagcggtcg aagcagtcgg ccagcgtcgt gagcgggccg
     4801 gacggcacgg cggccacctg gtcggccagc gcccggtaca tgcccggctc gtcccccagc
     4861 gcggcgagcc gcacggcctt cgccttcggc gggcggaacc agtacgcgcc gtggatcacg
     4921 gtcacgtact tgggcaggtg cttgttgatc gtgcgcgggc gggccatgtc aggctcctac
     4981 ggctttccag tcgagcttac cgcgcccctg gggctcggcc tcgaactgtg cgcggtggac
     5041 catgggacag ccgtccgccg gccgcgtgcg gaacttcacc cccatgttga cgagcgcctg
     5101 gcgctgccgg tcacgctgcc ggtagccggt cagctcgtag agctcgtcat cgttcagcca
     5161 gagcgacatc gggtcctcac cagggaatag ggcagccagg cgaagcgctg gaagatccgc
     5221 tcgcgcacgc gatagctgct cgcccgccgg aagtgtccga ggtagcttgc ccagatcgag
     5281 cgcaggtgct cgagggcagc gttggaggcg cgcagcccgg atggcctgac gtgctcgcgc
     5341 tcccaggcgg cgagtttcgc gcgggcgtgg gcgatcacgc gccggcgcac caggcggtgg
     5401 gtcgggaaca caacgtagcc gaggaagtcg atgccctcgg tcaggcgtcg gagcggcggg
     5461 ctcggcttca gctcgaggcg aagttcattc gcgaggaacc gttcgatccg ctcccgccag
     5521 gcgacgagct ggtcccggtc ggagtgcacc aggacgaagt cgtcgacgta ccgcaggtag
     5581 cgggtagtct tgagttcgtg cttgacgaac tggtccaggg cgtcgagata gacgttcgcg
     5641 aagaactggc tcgagagatt gccgatggcg atgccacaac cgggaggcgc gccctcgagg
     5701 cgtttgtgta gtggcaccgc ggcgcgctgc tccggcgtgc acgcgtagcg aacgcccgtc
     5761 tcggcgcaag agcgcgcgag cagggcatgg gtcgccttgc ggatgggcag cggcaggtcg
     5821 tgccgcccca tctcgcgctt gagcatccgg tagagcgtcg gtcggtggat ccggttgaag
     5881 cagttgcgga tgtcgagctg cagataccag ccgccgctct ggccgctctg tacctggcgc
     5941 acgaaggcct gcaggcgatc gacggcggcg tgggtgcctt tgccccgccg gttcgagtag
     6001 ctgtcgtgga cgaaccgcgg ctcgtagatc gcctcgaggc gcggcaccag gtagtggtgt
     6061 acgacccgat cggggaaggc cggcgcgtgg atctcgcgcg ctttcggctg cgaggcgatg
     6121 aagcaggtgc aggggctagg cgaccaggtc agggtattga gacgctcctg cagctcgagc
     6181 aggcggtcca tccactgggc gtcgaaggcg atctgatcga cgctcggctt cttgcctcgc
     6241 cgtgctgctc gccaggcagc gtggaggtcg cggaagctca tggcaccctg acactcaccg
     6301 gcgcgcaccg cgcgaacgag gccctcgttg ttctggtgat tccagttcga gttgccgttg
     6361 ttgaagttga cgtaccacgc ggagtccgaa ggggacggcg ctatcccgga cgcttgcgac
     6421 ccggccgcac atccgctggg gtagcacggc ttcgtcatgg atggaccccc gcaggggcgg
     6481 cccgggaact cagtctctga gcacgctggg ctggcacagg ggtcgccggc ccattctggc
     6541 tcttcaggtg cagttgcttc cgccagcctc cacactgccg gccgaggtcg gatgccaccc
     6601 gcgcaagggc ttcaaactcc ccgaagctct ggaatacttc gatctccttc cccaactgca
     6661 tcgtgagctt gagctcatcg atggcgatcc cgagctgatc cacccgctcg agctgatgcg
     6721 ctcgatcgcg ccaggcgcgc tgcgcacaac gcgcgacggc catcgcctgg gcgcgtagat
     6781 ccgcaccgac cgcgtacttg tggtagcgcg ggaagcgcct cacggcgcgc tccagatcga
     6841 ccaggagccg cgtcgagagc ttcacgatgg gtggcagacc gtcacgcatg gatcagcgtc
     6901 gcaggcctat agtcccaggg actgaccggc gcgcaccgcg cgaacgaggc cctcgtagtt
     6961 ctggtgacgc cagaacgagt aaccgttgtc gaagtagacg aaccacgcgg agaccgaagg
     7021 ggacggcgcg cagggcgtcg acgtccagta ccagttgcca gcctctgtga agaagctcgt
     7081 atcgatggcc ggatctgcgc ggctgtagtc caccagcgtg agcagctcgg tcacggtcgg
     7141 caggcgccag cccgcctggc cgcacagagc gcacgattcc gctgcctgat gcgccgcctt
     7201 ccactggtgc cggccctcga ggggcgcgcg cgtccagacc aggtccgtga ccgcatcgtg
     7261 cacggcgacc cactggccgg agtccatggc catggcgcga ccgtcgtcgc tgatcttcgt
     7321 gaaccgcgga cccgcgagcc gtaccggtgc cgcgggtgca accccatcgt ctgcgcatag
     7381 cgtcgacacg atccgcagca cctccgctgc gggcgcctga tccaggcggc gcttcaccac
     7441 ctgcttcaat tcctgactta acgagttcat ctgacgctcc tcgcccaaag gccgaaatga
     7501 ctaaccggcg cgcaccgcgc gaacgaggcc ctcgtcgtac tggagagtcc agctcgagtc
     7561 gccgtcgtag aagtagacgc accacgcgga gtccgaaggg gacggcgccg ccacggtgcc
     7621 gctccagtac cacttggatt ggcactccgg aaagaatgcg atgtcgatcg cgggcgcggt
     7681 gcgcgagcga tcggcaagca ggaacagttc ctcgacggtc ggcaggcgcc acttccagcc
     7741 gcagagatcg agcttctcgg cgtaggcctt cgccttcttc catcgcatgg gcttcttcgc
     7801 ctcgcccgcc gaccagacga gcttcgtcgt ctcatcgcgc acggcgaccc agttcttcgc
     7861 atcggccggc aggtcctgcc catcgaccga gatcttcgtg aagcggctca tcgttcgttc
     7921 ctcatcaggt ggtgagttgt cggtggagga cctcgacccg gtgcgccgtg cgcgcgagga
     7981 cgaagtcgtg cttgatctgc agtgccggat ggcgggcgct cgagccaagg cgtgcagcga
     8041 ggtccgtgcg tgtgaagcct tcggcaatca gctcgttcag gagcttccag gtgcggcggg
     8101 cggacacgag ggcgtgatcc gacgcgcagg tcttcgtgac cgcgagaatc cgccgctcgg
     8161 tgcgggcgcg gatgcgcgcc tttcgtccgc tgcggatctc cgagatcacc gtctcggcga
     8221 cgtcggaggc ggccgccacc gcgcgacgcc caatgccgat ccgcgagagc ttgatcaggt
     8281 gcgcccgcgc cgcggcggca tcgacgatgc cgttccagtc gcccgccgcc cgtgccgcct
     8341 ggcggtgccg ctcgtagtcg ctgttcgagc ggcggcactt gaagcagcgg cagccgccca
     8401 ggtagcgcag gcgatcgccg tgcggccgct gcgcggcgag ttcgaacgcc ggccgaaggc
     8461 ctcgcgcttc gagcatcagg ttcatccgat cagcgcgatc cagagccagc ccgcgatcga
     8521 ggcggcgccg acgagggccc agccagcctc acgcagaagg tgcgctcgcc ccccgcggac
     8581 acccggtcgt cgctgatctg caggccgccg cggcggtaga gccgcaacca caacgaggtg
     8641 tggacgggcg tggtctttcc ggcgtgcgtc acgctgatgc ccgcccaggc cccggaccag
     8701 gccatctgct tcacgttgag cacggcgccg gcgcgcagca tcgatctcag cgacgccatc
     8761 gacgtgacga ggaaatccga gctcatgggg cgccctcatg ccggcttctc gcttcggaag
     8821 gccgcgggca cctgcagggt ctcgaactcg atcctcaggc cgttcggcat cgtcggatcg
     8881 ctcgtcacga tcgcgcgggc gcgcaacgcc cccgcgtgct gggccaggcg ccagacgctc
     8941 cactgcgcca tccgcgcggc gttgcgcctc gtgatctgct cgctcaggag ctcgagcgcg
     9001 tcgtcgatcg cggcctcgcg ctcggcctcg ggcaggggct cgatcgccgc ggcgacggcg
     9061 ttcatgccgc cacctgccgc atcgcgcgct ggtcggcgac ggtcacgacg cggccgtcgg
     9121 gccacaccga ggcgtagacc gggtgatcct tcagcaggct ctcgagcgtc gccttggtgc
     9181 cgacgtggaa cgaggggccg tcgaggacga cgaagacgtt ctcacggcgc gacgccgcgg
     9241 cgtccttcgc gagatcgatg gcatggtcga gggtcatggc tgcctccatt actggaagca
     9301 aactatcacc acatggttat tttcgcaaca ccaatcggtg aatctaacta tgaagccgtc
     9361 gtgacgagcg ctatgcttgg cccatggaga gcgcgccaca gcgggtgatc gtcaccgacg
     9421 tcgatatcaa gttcggcagc atggtgacac tgttgatcaa gctggccttc gccgctctgc
     9481 cggccgcgat cttcgtaggc gcagtaggat tcatcgctgt gatgcttgct ggtggcatcg
     9541 gcgggtccgt catctcggcg ctcaagggtg gcggaacgcc ggcaagcatc gaagcgccga
     9601 ccccaagaga ccccaagata gacgaatcac tgattgcggc ccggcgcctg ctggctgcgg
     9661 gcgatcggga aaacgccgag gaaatcctgc gccgcgcgat gctaacgggc gccggctcca
     9721 tcgaacagcg acaagatctc atcgagaccc tgcgacgagc tcaagccgct aactgacctg
     9781 ctgagccgcc gtctgccgtt tcgccttcgt ccgggcgtcc cgcttctgcg ccgctaccag
     9841 acataggatc tgctctcgga cgcacgatcg ctggggctcc tccagcttcc cccattcccg
     9901 cccaacttca gcctcttcag cactcggact gatcgaccag ctatgcgttg cggctgcgcg
     9961 gtcggcaacc accggaccag attccgcgtc aagccatccc ttccccattt gcccgcgtcg
    10021 ctcgagatca cgggctatcc gctcgccgaa ggacttcctg ccgctgagca tgtcctgaat
    10081 ctgaggctga ctcttgccga cggaagcggc gaatcgcgac gccacgccat cgaacttgtc
    10141 gtcgatccat tggcgcagtc tattgcgcct aacttgttga ataaccacgg gttcattttg
    10201 gggccgcata caccgtccgg tgaaatcccc ggttggtgtt gcatatataa tccccatatg
    10261 gtgatattga gcggtgacgc tcaaggaatt ccttcagacc ctctccctgg ccgacaggtc
    10321 tgtattcgcg cagtccgtcg ggtcctcggc gggccatctc cgcaacatct cctacggcta
    10381 caggccatgt tccgaagggc tggccatcga gatcgagaag ctctcggcgg gcatcgtccg
    10441 atgcgaggac ctctgccctg acgtcgattg gtcatatctg cgcaattcac agcgcgagct
    10501 tgcgccgtga cccgttccac gggcatccac gattcgggga acgccatgca ggcggtccta
    10561 ttccatgact cgatcgccga tgccatcgga acggcgatag cagcgatcgg aggcccgaag
    10621 aaggccggcg gcgctctctg gcccgctgat cctgtcgcga gatccgaggc tcgggtgcgc
    10681 tcatgcctca atgcagagcg cgcggagaag ttctcacccg acgaagtgat gcagcttgcg
    10741 cggctcgcgc gagctgcagg cgatcactcg atcatgaact acctcgcggc cgagctcggc
    10801 tacgagatcc gcccgctcga tccgcaggac cagatggcgg cgctgcagca gcagtacatc
    10861 gacgccgcac gttcgattcg ttccatcgct gagcgcatcg aacggatcca ggtgagggcg
    10921 gtcggatgag tggcacaccg acactgcgcg tcacggccga cgtcgggcca ctgatgcgcc
    10981 cgctctcaga acttaagggt gagcttgatc ggtatcgaga gcttgcggtc gatctccctc
    11041 gcgctctgca gggcctcctg gtagccgcgg ttcagtccgt cggtgtcgag cgtgatcgtc
    11101 gctcggcacc ccgggcagtc catttcgtgg tgcgtccgaa gagaccgcag ggtcttcgtg
    11161 atctggtgcg agcacttcgg gcaatcaacc gtcagctcct gcgcatccag cgtctgggca
    11221 acctgcgctt ccatcgatcc catgtcggcg gtcctcgtgt tggaactggg gtggtggttc
    11281 gtcccaagtc tagcgcgagg cccgccggct cccggaaggc ccgccgatga ggggcgccgt
    11341 cgccaagggg ctgcgccgcg aagccgctgc gcgcacggtc ggccagccgg ccaccggcta
    11401 cacgtcccgt ccgcagacga attcgaaggg gcaggtcgtt ggccgtgccc tgatcgttca
    11461 cccatcaacg acgcgcggtg tgtaccgcgc gttgaagcgt tcccgccgta ccgctaggag
    11521 gccgcagcgc tgatcgccgg gccgcgcagg ccctcgggtt ctcaacagtc caccagtgag
    11581 tgcaccggcc gggacccgaa gacatcaagc cgccaggaga tgaggccagc gctgtaacgc
    11641 gttggccggc gtagcacagc ggcacggaag gggattgtgg agagacccgg ctacgcgcac
    11701 ggcgatcgtg cgagctctcc gacgcggacc gacgctgggg acgcggggcg tgatgacgac
    11761 cgtggggccg agggcctggg acgtcggcgc aagagaccag ccgcaccagc gggtaggggt
    11821 ctctgcgcct tcgtcccgca gaaccgaggg tctgattccg gcgccggatg cttgctccgg
    11881 cgcttgaggg aaggaagagg ggaggactgc cttcgatgaa cgaaggcact acgcgcgcat
    11941 gagcattgca ccgcacatct cgccggttga ttccacgcgt cttgcccacg cgatggcgaa
    12001 gctgcaccgc cggacggcag aggcggaggc ccggcgacga gctctcgatg ttccatgtga
    12061 aaccccacca ccgtctccgc cggccgaaga gccggcgcca cctccgctga cctctgcctg
    12121 tgatccccca tcccctgctg cagaggtcgg cagcgatcct cccccgggtc tcaccaaccc
    12181 gggggaggaa tcgccttcgc cagcgcgtcc gttcatggct ctgctgctcg agtggttcgc
    12241 cgcccgcgtg aagaagacgc tgacgtggga gttgcgccgc gcttcggtcg ggaaggacgc
    12301 ctactcgctc gagacgttcc tctgcgcgga gttcccgggc ggcccgtaca cggttgttcg
    12361 ctccgccggc ttcgcggacg acgcctggta cgaactcttc gccgcggaga agtccgttgg
    12421 cgcctggctc acgagctcgc gtgagggctg cgcgctcgcg gagtggcacg ccgcgggggc
    12481 gcccgcgcta tgagcgatcc acgccaggtg gtgatcgatg cgctccgcag cgggccgaag
    12541 aactaccaga gcctgcgcat ggccacccac ctcgagcccg gggccatgga cgctgcgctc
    12601 acggccttga agcgcgacgg cctcgtgaag atcgacatgg gcttctggga gctggtggcg
    12661 gaaacgcccc agccgcgaaa gaccctcgaa gtcaccatct ccggccccgt cgcatccggg
    12721 gactcccttc tcgaggttct ccacgccgcc gcagatcgcg acgtcatggt gactgcgccc
    12781 actgatccgc cgcccacatc cgaggaccac gatcccatgg ccagcacgaa gcattgcaag
    12841 cgctgcgaca agcgcaagcc cgtctccgcc ttcgggaaga acgccacccg ctcggatggc
    12901 ctgcagacct actgccggcc atgcatgaac gaggcgacgt cgaagcgcaa ggacgctccc
    12961 gcccgggcga agaaggtcgc ggcgcccgtc gtttcgacga acgccgaagc ggagtcccct
    13021 gagcccgaga accgcgagat ccactgcgac ctgttcttcg tcacccaccg caccgtctcg
    13081 gcgcaatgga gcggcgagta cgtcgcgatc gacgtgcgcc aggacggtgt cagcgagcgg
    13141 gtggtgatca cgcgccgcga gctgcgcgcc attgcggcgc aagacctgaa cgagtgagga
    13201 accccatgct gcaactcgat gccttcggtc ctccaccgac tccccgccgt cgggaggcgc
    13261 gcctgaagcg cgatctcggc atgatccgcg ccgaactcag ccaggagcgc gcctcgcacg
    13321 actggaacct ccaggccgcg atgtacctgc gcgagtacgc caggcggatc gcccagggcc
    13381 agccgttcaa gatcgaggat gcccgcgagg cgagcgcctc ccgggtgccg gtcccgaaga
    13441 accagaagtc ttggggcccc gccgtgatgt tcgcggcccg ccagaaatgg atcaagcggg
    13501 cggaagggcc ggatggacgg ccgatgtacg accgtgcgcg cagttccaat ctctcgccga
    13561 agcccctgtg ggtggccggt gagtagggcg ccccgggtgg tgcgcgacag tctcgggctg
    13621 cgccaggggc tcgtcgccgc
//
LOCUS       DAORPG010000002        11483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_200_length_11483_cov_3.251750, whole genome shotgun sequence.
ACCESSION   DAORPG010000002 DAORPG010000000
VERSION     DAORPG010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 11483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11483
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200_length_11483_cov_3.251750"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="PLX85_00130"
     CDS             <1..931
                     /locus_tag="PLX85_00130"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRC61613.1"
                     /translation="LGLLERINPHTRVMIELSHTQLETVTRSPELACVTNVTPNHLDH
                     YSWSAYVALKRRIFQFQRPEDTCVFNLDNEVSASFARETPGRVAYTSLVRSLPGDGVA
                     LVDGVVTRFEGSTSFAVAARSDIRLRGQHNVENVLSAVALASRLGVSDQQAAAAIRTF
                     TGVPHRLEPVATIADVVYINDSIATTPERTLAGMRSFEQPLVLLLGGRHKYLPVGELA
                     SEATRRCRAVIAFGEAGEMFASAVRDADGGSSVQVEQVADVETAVRAAHRLAQAGDVV
                     LFAPAGSSFDAYPNFERRGAAYREAVRQLAGED"
     gene            931..2124
                     /gene="ftsW"
                     /locus_tag="PLX85_00135"
     CDS             931..2124
                     /gene="ftsW"
                     /locus_tag="PLX85_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HRC61614.1"
                     /translation="MARRPVEAAVRRHGPDYLLLAVLTVLLVIGLIAVYSSSYALGYA
                     QFGDPNFFIKKQVLFAVLGLVGMFIAMNVDYRILMKLSPLLMLFALLGLAAVLLPGIG
                     FEQNGARRWVALGPLPPLQPSEFAKVAVLIYMAAWLAAKGETVKDFSLGVMPFVGMVG
                     LIGALIMLEPDMGTAMMVALITGTLFFVAGARLTHLLALVGSGLVMAVILILSHGYRA
                     DRLLAFTSAEDDPTGVGFHALQMLVAFGSGGIGGLGLGVSRQKFFYVPGSHTDGVLAI
                     VGEELGFIGVVVVLLLFVVLLARGLRIAMRAPDRFGSLLATGVIAWIALQLLINVGGV
                     TRSIPLTGIPLPFLSYGGSSLMALLTAVGVLLSVSRYAAIDDPERLPPTRGVVRPSPT
                     PEAGR"
     gene            2121..3191
                     /locus_tag="PLX85_00140"
     CDS             2121..3191
                     /locus_tag="PLX85_00140"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HRC61615.1"
                     /translation="MKYALAGGGTGGHAYPSITVGEELRRRGHELVYYGTERGPEHAL
                     ADSAGIPFRGMAASQVRGGPRRMARGGLNLVRGTRHVAAHMRADRPAALFATGGYAAA
                     PVGLAARRHRVPMLVFLPDVHPGWAVRILNRFATGVACSVDAALPSLPRRKSVVTGYP
                     VRAQFLSATREEGLRRFGLDSALPTVLVTGGSLGARAINQAVAQSLPRLLARAQVLHV
                     CGRHEEAALRAERERLPAQLCDRYHLLAYTDEMAWAMAASDLAVARAGASTLGELPMA
                     GLPAVVIPGGFSDQQLNARYLEARGAAVTLGNDQLDRLEGIVLGLLEDEGKRARMAAA
                     MRGLAHPEAASQLADLLEAMAA"
     gene            3191..4609
                     /gene="murC"
                     /locus_tag="PLX85_00145"
     CDS             3191..4609
                     /gene="murC"
                     /locus_tag="PLX85_00145"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRC61616.1"
                     /translation="MVRLKARSESNGFTIPQRVHLIGAGGMHMSAIGQILLARGHIVT
                     GSDIAASEHVERLRALGATIYEGHAASNLGRAELVVTTVAARDDNPELLAALSLGVPV
                     LVRAEMVQKLIADRDVLAVAGTHGKTTTTTLLAWMTVRGGLDPLVLAGGDSREIGSNA
                     RDGAGRLAVVEADEYAEAFLQYEPRYALILNVEADHLDYYGTEERFVEAFRAFSKRVQ
                     PEGTLFVSADSPYAAEIGAERAREGRRVERFALGDSEAEWRASQLRGNDRGGLDFLVQ
                     LDGAEVGRMSLQVPGRHNVSNALGALAVAMRAGVDFHRAAQAAAEFTGAHRRFEHLAD
                     VAGVTLMDDYAHHPTEVRATLQSARARFPGRRIVACFQPHTYSRSRYLLDGFRSCFES
                     LDRLYILGTYAARETSEAGLSAQELAAEIKKPAPTYVETFEEAAERIASELQPGDVFF
                     TIGAGNVDALGPMVRQLIEARP"
     gene            4606..5676
                     /locus_tag="PLX85_00150"
     CDS             4606..5676
                     /locus_tag="PLX85_00150"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HRC61617.1"
                     /translation="MSGRTRVGVVFGGRSPEHAVSVVSARSILREADPQRFEAVPFGI
                     SRGGSWLTPAETRRRLERVAVGETDTLGDDSDGQLLRAHEVLDALADLDVVFPIVHGR
                     LGEDGTLQGLCELADKPCVGSGVAASAVGMDKALMRAAFAAAGLPQPRYAVLRDDDAV
                     QPPPDLLRAIEGEIGYPCFVKPANGGSSLGVSKARTREDLIEGAATAARYDRKILIEE
                     AIGGREVECAVIGNAHPSASPLGEIRPKAEFYTYEAKYSDDTTELIVPAALADDTAMR
                     VQEAALAAYAAVDCAGMARVDFFVTPDDDIRIIEVNTLPGFTPISMYPRLWQHAGMSY
                     AALISRLVDLAVERHEETRRYA"
     gene            5783..6478
                     /locus_tag="PLX85_00155"
     CDS             5783..6478
                     /locus_tag="PLX85_00155"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HRC61618.1"
                     /translation="MRGYGLATLGAALLVVGGWWLWTSSALRVGTVIVVGTEVVDANA
                     VVSAADLHGRSIVTLDTAGAQKRILAIAGVDAVQVHRDWPRSAVIEVREKQGWGYWQA
                     AGIRSLIDANGRVLDKARLPGDAAPTIYEAGGTPLQTGATADPDTVALVTRMLGDGTF
                     ERLGHAPLRFEFDRSRGLTVRLQDGPAAVFGDSHDYEFKVAAWAAILDRIKTEQIKAA
                     EVDLRFGRELVVR"
     gene            6478..7719
                     /gene="ftsA"
                     /locus_tag="PLX85_00160"
     CDS             6478..7719
                     /gene="ftsA"
                     /locus_tag="PLX85_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRC61619.1"
                     /translation="MANPTFAAIDVGTTKVCTVVAEVTSAGDLRILGVGVGPSAGLTK
                     GMVDNIHAAIEAIASSVERAERASGTRILSAHVGIAGPHVSSMNSRGIVAIADPRHPI
                     TESDVHRALESARIVNVPNNREVIHVVPRYYVVDGQDHVVDPMGMFGSRLDVETHVVT
                     AGSSAMHNLMQCVEGAGVRVDSIILEPLASAEAVVDEEELNQGAVICDIGGGTTDIAV
                     FVQGAVVHTAVLPVGGAHMTRDLVVALRVPQPAAELAKRRYGHAIPSLVDEDEEVEVD
                     AFGSEGRKSVSRRLIAEVLQARTEELFEMVQAEIRRGVDQDVLSAGIVLTGGAASLPG
                     IEMLAEDVLGLPARVGRPRHLQGLSDLLNDPAYATSVGLLRWSLKEREIMLRSSPGVP
                     TASFGGLLRKVANLMRVVLPQ"
     gene            8349..9446
                     /gene="ftsZ"
                     /locus_tag="PLX85_00165"
     CDS             8349..9446
                     /gene="ftsZ"
                     /locus_tag="PLX85_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRC61620.1"
                     /translation="MDDNFDALPPIKVVGVGGGGSNAVNRMISARLPGVQYVALNTDM
                     QALSHCGAEIKVRIGDRLTKGLGAGSEPLRGARAAEESREAIAEALQGAEMVFVTACL
                     GGGTGTGAAPIVAEVARELGALTIGVVTKPFTFEGAKRRQQAEEGVRDLQEKVDTLIV
                     IPNDRLLQICDEKVSMQDAFRIADDVLRQGIQGISELITVPGIVNLDFADVRKIMSDA
                     GPALMAIGTGRGEHRAVDAAKMAITSPLLEVDITGATGVLFNVTGPANLGLHELNAAA
                     RVIAEVVDPEAEIIFGTAVDESLQDEVRITVIGTGFTGVRPAAMRPPRDEPMTADPKP
                     IRLADVQPEPSLDQLTEADLPTFLRRTFPTR"
     gene            9650..10231
                     /gene="nrdR"
                     /locus_tag="PLX85_00170"
     CDS             9650..10231
                     /gene="nrdR"
                     /locus_tag="PLX85_00170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRC61621.1"
                     /translation="MKCPYCGHMASKVIDSRTSDDGVRRRRECQACNERYTTYEQFQR
                     SVVMVIKKDGRREEFQREKLLHGLRSAVQKRPLPTGSIDAIVESIEHRLMTSGWSEVP
                     SRAIGEMVIKHLKALDPIAYIRFASVYHKFVSVDELLSELTQLAQSPPPTADQPRLFE
                     DELSELVDGDDSASPAPPPPTPIERAPSILARM"
     gene            10310..>11483
                     /locus_tag="PLX85_00175"
     CDS             10310..>11483
                     /locus_tag="PLX85_00175"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HRC61622.1"
                     /translation="MVLSATVTTHPTALTENARTVLARRYLLRDESGTIVETPEQLFA
                     RVAEAIASVEATDEARQRWAQRFYDAMASLRFLPNSPTLMNAGTGRGTLAACFVLPIE
                     DTLESIMSTAQAAAMVQKYGGGTGFSFSRLRGEGEPIATTHGAACGPVSVLRHYDDVS
                     RLVTQGGKREGANMGVLRVDHPDIRAFIHAKDDGVSAQRFNLSIAVTDEFMRAAATGG
                     SLELRDPRDGSERGSEDAAALLSDIARSAWATGDPGLVFLDTINRDNPTPQIGAIEAT
                     NPCGEVPLLPWEACTLGSINVARFWDAEAGDLDWDTLGETVTLGVRFLDDVVEANTFP
                     SKEIAAAVRGNRKIGLGVMGFADLLITAGLPYDSEEALVLANRLGRFISGQADEASA"
BASE COUNT         1801 a         3744 c         4095 g         1843 t
ORIGIN      
        1 gctcgggctg ctggaacgca tcaatcccca cacgcgggtc atgatcgagc tgtcgcacac
       61 acagctcgaa accgtgacgc gaagcccgga gctggcctgc gtcacgaacg tcactccgaa
      121 ccatctcgat cactactcat ggtcggcgta cgtcgcgctc aagcgacgga tcttccagtt
      181 ccaacggccc gaggacacct gtgtctttaa cctcgacaac gaggtcagcg cgagcttcgc
      241 gcgagagaca ccaggacgcg tggcctacac ctcgctggtg cggtcgctgc cgggggacgg
      301 ggtggcactg gtcgacggcg tggtgacacg cttcgagggc agcaccagct ttgccgtcgc
      361 cgcgcgctcc gacattcggc tgcgcggtca gcacaacgtg gagaacgtgc tgtcggccgt
      421 cgcgctcgcg agccgcctcg gcgtgtccga tcagcaggcg gcagccgcga tccgcacgtt
      481 caccggcgta ccgcaccggc tcgagccggt agcgacgatc gccgacgtgg tctacatcaa
      541 cgattcgatc gcgacgaccc ccgagcggac gctcgccggg atgaggtcgt tcgagcagcc
      601 actcgtgctg ctcctcggcg ggcggcacaa gtacctgccc gtcggcgagc tggcaagcga
      661 ggcaacacgg cgctgccgcg cggtgatcgc gttcggcgaa gcgggcgaga tgttcgcctc
      721 ggcggtgcgg gacgccgacg gcggttcgtc cgtgcaggtg gaacaagtcg cggacgtcga
      781 aacggccgtg cgcgccgcgc accggctcgc gcaggccggg gacgtagtgc tcttcgcgcc
      841 agccggcagt tcgttcgacg cttacccgaa tttcgagcga cgcggtgcgg cataccgcga
      901 agcggtccgg cagctagcgg gggaagactg atggcgcgcc ggccggtcga agcggcggtg
      961 cgccggcacg ggccggatta cctcctgctg gcggtgctga cggtcttgct cgtgatcggg
     1021 ctcatcgccg tctattcctc ctcgtacgcc ctcgggtacg cgcagttcgg agacccgaac
     1081 ttcttcatca agaagcaggt gctgttcgcg gtgctcggcc tcgtcgggat gttcatcgcg
     1141 atgaacgtgg actaccggat cctgatgaag ctcagcccgc tgttgatgtt gttcgcgctg
     1201 ctcgggctgg ccgcggtgtt gctgcccggg atcgggttcg agcagaacgg agcgcggcgc
     1261 tgggtcgcgc tcggaccgct gccgccattg cagccgagcg agttcgcgaa ggtcgcggtg
     1321 ctgatctata tggcggcctg gctcgcagcc aagggcgaga cggtcaaaga cttctcgctt
     1381 ggcgtgatgc cgttcgtcgg gatggtcggg ctcatcgggg cgttgatcat gctggagcca
     1441 gatatgggca cggccatgat ggtcgccctg atcaccggaa cgttgttttt cgtggcgggc
     1501 gctcgcctca cccacctgct ggcgctggtg ggcagcggcc tggtgatggc cgtgatcctc
     1561 atcctcagtc acggctatcg ggccgaccgc ctgcttgcct tcacctcggc cgaggatgac
     1621 ccgaccggcg tcggcttcca cgcgctccag atgctcgtcg cattcggttc gggcggcatc
     1681 ggcggtctgg gcctcggtgt ctcgaggcag aagttcttct acgtccccgg ctcccacacc
     1741 gacggcgtgc tcgcgatcgt tggcgaggag ctgggcttca tcggtgtggt cgtggtgctg
     1801 ctgctgttcg tggtgctgct cgcgcgcggg ctgcggatcg cgatgcgcgc gcccgaccgc
     1861 ttcgggtcac tgctcgcgac gggggtcatc gcgtggatcg cccttcagct gttgatcaac
     1921 gtcggcggcg tgacgaggtc gattccgttg acgggcattc cgctgccgtt cctgtcgtac
     1981 ggcggctctt cgttgatggc gctgctcacg gccgtcggcg tcctgctatc cgtctcgcgc
     2041 tacgcagcga tcgacgatcc ggagcgactc ccgcccaccc gaggggtcgt gcgaccgtca
     2101 cccacacccg aggcgggacg atgaagtatg ccctcgcggg tgggggaacc ggcgggcacg
     2161 cgtacccctc tattaccgtg ggcgaggagt tgcggcggcg cgggcatgag ctcgtgtact
     2221 acggcaccga gcgcgggccc gagcacgccc tcgccgactc tgcgggcatt ccattccgcg
     2281 ggatggcggc ctcacaggtg cgcggaggac cgcggcgcat ggcccggggc ggcctcaacc
     2341 tcgtccgtgg cacgcgacat gtggccgcac acatgcgagc tgaccgtccg gccgcgctgt
     2401 tcgcgaccgg cggttatgcc gcagcgccgg tgggcctggc ggcacggcgc caccgggtgc
     2461 cgatgctggt gttcctcccg gacgtacatc ccggctgggc cgtgcgcatc ctgaaccggt
     2521 tcgctactgg cgtcgcctgt tcggtcgatg ccgcgctccc ctcgttgccg cggcgcaagt
     2581 cggtggtcac gggctatccg gtgcgcgccc agttcctctc ggccacacgc gaggaagggc
     2641 tccggcgctt cggactcgac tcggcgctac cgacggtcct cgtcaccggc gggtcccttg
     2701 gcgcccgggc catcaaccag gccgtcgcgc agtcgttgcc gcggctcttg gcgcgcgcgc
     2761 aagtcctgca cgtctgcggg cggcacgaag aggccgcgct gcgagcggag cgcgaacgac
     2821 tgcccgcgca gctctgcgac cgctatcacc tgctggctta caccgacgag atggcctggg
     2881 cgatggcggc gtctgacctc gccgtggcgc gggccggtgc gtcgacactg ggtgagctgc
     2941 cgatggccgg gcttcctgcg gtggtcatcc cggggggctt ctcggaccag caactcaacg
     3001 cgcggtacct cgaggcgcgg ggcgccgctg tgacgctcgg caacgatcaa ctggaccgac
     3061 tcgaggggat cgtcctgggc ctcctcgaag acgaaggcaa gcgggcacgg atggccgcag
     3121 cgatgcgcgg cctcgcgcat cctgaagcag cgtcgcagct ggcggatctg ctggaagcga
     3181 tggcggcctg atggtgcggc tcaaggcgcg ttccgagtcg aacggcttca cgatcccaca
     3241 acgcgtgcac ctgatcggcg cgggtggaat gcacatgtcc gccatcgggc agatcctgct
     3301 cgcccgcggc cacatcgtga ctggatctga catcgcggcg tcggagcacg tggagcgact
     3361 gcgcgccctg ggcgcgacga tctatgaggg tcatgcggct tcgaacctcg gccgcgccga
     3421 gctggtggtg accaccgtcg cggctcgcga cgacaacccg gagttgctcg cggcgttgtc
     3481 actgggcgtg cccgtgctgg tgcgcgccga gatggtgcag aagctgattg ccgaccgcga
     3541 cgtgctcgcc gttgcaggga ctcacgggaa gaccacgacg acgacgcttc tggcgtggat
     3601 gaccgtccgt ggtggccttg acccgcttgt gctcgccggc ggcgactccc gtgagatcgg
     3661 cagcaacgcg cgcgatgggg ccggccgcct ggcggtcgtc gaggcggatg agtacgccga
     3721 ggcgttcttg cagtacgagc cccggtacgc gctgatcctg aacgtcgaag cggatcacct
     3781 cgactactac ggaaccgagg aacggttcgt ggaggcgttt cgcgcttttt cgaagcgagt
     3841 gcagcccgag gggacgctct ttgtgagcgc ggattcgccg tacgctgcgg agatcggggc
     3901 ggaacgcgcg cgcgaagggc ggcgcgtcga acggttcgcg ctcggggact ccgaggcgga
     3961 atggcgggcc tcgcagctcc gcggcaacga ccgcggcggt ctggacttcc tcgtgcagct
     4021 cgacggcgcc gaggtgggcc ggatgtcgct ccaggtgccg ggtcgccaca acgtgtcgaa
     4081 tgcgctgggg gcgctcgccg tagcgatgcg ggcgggcgtg gacttccatc gcgccgcaca
     4141 ggcggcagcc gaattcacgg gagcacaccg gcgcttcgag cacctcgccg acgtggcggg
     4201 ggtgacgctg atggacgact acgcgcacca cccgacggaa gtgcgcgcga cgctccagag
     4261 cgcacgcgcc cgcttcccgg ggcggcggat cgtggcctgt ttccagccac acacgtactc
     4321 gcggtcgcgt tacttgctcg atggcttccg aagctgcttc gaatctctcg atcggctgta
     4381 catcctcggc acgtacgcag ctcgagagac gtccgaggcg ggcctgtccg cgcaggagct
     4441 ggcggcggag atcaagaagc ccgcgccgac gtacgtggag acgttcgagg aggcggccga
     4501 acggatcgcc tcggaactgc agcccggaga cgttttcttc acgattggcg ccggcaacgt
     4561 cgatgcgctc gggccgatgg tgcggcagct catcgaggcg cggccatgag cgggcgcacg
     4621 cgcgtcggtg tcgtgtttgg cggccggtca cccgagcatg cggtgtcggt cgtctcggca
     4681 cgatcgatcc tgcgggaggc ggacccgcag cgctttgagg ccgtaccgtt tggcatctcc
     4741 cgaggcggga gctggctgac gcccgcggag acgcggcgac gtctcgaacg ggtagccgtt
     4801 ggggagaccg acacgctcgg cgacgacagc gacggccaac tactacgggc gcacgaagta
     4861 ctggacgcgc tggccgacct cgatgtcgta ttccccatcg tgcacggtcg cctcggcgag
     4921 gacggcacgc tgcagggcct ttgcgagctg gcggacaagc cctgtgtcgg atcgggcgtg
     4981 gcagcgagcg cggtcgggat ggacaaggcg ctcatgcgcg cggccttcgc cgccgcaggg
     5041 ctgccccagc cccgctacgc agtcctgcgc gacgacgatg cggtccagcc gccgccggac
     5101 ctgctccgcg cgatcgaggg cgagattggc tacccctgct tcgtgaagcc ggccaacggc
     5161 ggttcctcgc tgggtgtgag caaggctcgg acgcgcgagg acctgatcga gggcgccgcc
     5221 accgctgccc ggtacgaccg gaagattctg atcgaagagg cgatcggtgg tcgcgaagtg
     5281 gaatgtgcgg tgatcggaaa cgcgcacccc tcggcgtccc cgctgggcga gatccgtccg
     5341 aaggccgagt tctacaccta cgaggccaag tactcggacg acacaactga gttgatcgtc
     5401 cccgccgcgc tcgccgatga caccgccatg cgggtgcaag aagccgcgct tgcggcctac
     5461 gccgccgtcg actgtgcggg catggcgcgt gtcgacttct tcgtgacgcc ggacgatgac
     5521 attcgaatca tcgaggtgaa taccctgccc gggttcacac cgatctcgat gtacccgcgg
     5581 ctgtggcagc acgccggcat gtcctacgca gccctcatct caagactcgt cgaccttgcc
     5641 gtcgagcggc acgaggagac gcgccgctat gcgtagaccg atcacgcatc ggccgccgcg
     5701 ccggcgattg tcgcgattca ctctcggtgt cgcttcttcg cggccacgcg gactcgacgt
     5761 cccctcgatc gactggcagg cattgcgggg gtacggcctc gcaacgctgg gcgcagcgct
     5821 gctcgtggtc ggtggctggt ggctgtggac cagcagcgcg ctgcgagtcg ggacggtgat
     5881 cgtcgtgggc acggaggtcg tggatgcgaa tgcggtggtc agcgcggccg acctgcacgg
     5941 ccgctcgatc gtgacgctcg acacggcggg agcacagaaa cggattctcg cgattgctgg
     6001 cgtcgatgcg gtgcaggtcc accgcgattg gccgcggagc gcggtgatcg aggtgcgaga
     6061 gaagcagggc tggggctact ggcaggcggc tgggatccgg tcgctcattg acgcaaacgg
     6121 gcgcgtgctc gacaaggccc gcctgccggg agacgccgcg ccgacgatct acgaagcggg
     6181 cggaacaccc ttgcagaccg gggcaactgc ggatcccgac acagtcgcgc tcgtcacccg
     6241 gatgctcggc gacgggacgt tcgagcggct gggtcacgcg cccttgcgct ttgagttcga
     6301 ccgctcgcga ggcctcaccg tgcggctgca ggatggcccc gcggcggtgt ttggcgactc
     6361 gcatgactac gagttcaagg tcgcggcctg ggccgcaatc ctcgatcgca tcaagacgga
     6421 gcagatcaag gcggcggagg tcgatctgcg tttcggacgg gaactggtgg tgcgctgatg
     6481 gccaacccga ccttcgctgc catcgacgtt gggaccacga aggtgtgcac ggtcgtcgcc
     6541 gaggtgacgt ccgcgggcga ccttcgcatc ctcggcgtcg gtgtggggcc ctcggccggt
     6601 ctgaccaagg gaatggtcga caacatccat gcggcgatcg aggcaattgc gtcctcggtc
     6661 gagcgtgccg aacgcgcctc gggaactcgc atcctgtcag cgcacgtggg catcgccggg
     6721 ccgcatgtgt cctcgatgaa ctcgcgcggc atcgtcgcga ttgccgaccc gaggcacccg
     6781 atcaccgaaa gcgacgtgca ccgggcgctg gagagcgcgc gcatcgtcaa cgtgccgaac
     6841 aaccgggaag tcatccacgt ggtgcctcgg tactacgtcg tggacggcca ggaccatgtc
     6901 gtcgacccga tgggcatgtt cgggtcgcgg ctggacgtcg aaacccacgt cgtgaccgca
     6961 gggtcgtcgg caatgcacaa cctgatgcaa tgcgtcgaag gcgcgggagt ccgagtggac
     7021 tcgatcatcc tcgagccgct tgcctcagcc gaggcggtcg tcgacgagga ggaactgaac
     7081 cagggcgcgg tgatctgcga catcgggggc gggaccacgg acatcgccgt cttcgtccag
     7141 ggtgccgtgg tccatacagc cgtgttgccg gtcggcgggg cgcacatgac gcgcgacctc
     7201 gtcgtcgctc tgcgcgtgcc gcagccagcc gcagagctcg cgaagcgccg ctacgggcat
     7261 gcgattccct cgctcgtcga cgaggacgag gaggtcgaag tcgatgcctt cggctcggag
     7321 ggccgcaagt ctgtctcgcg ccggctgatc gcggaggtgc tgcaggctcg caccgaggag
     7381 ttgttcgaga tggtgcaagc ggagatccgc cgcggcgtcg atcaggacgt gctctcggcg
     7441 ggcatcgtgc tgaccggcgg ggcagcgtcg ctgcccggca tcgagatgct ggcggaagac
     7501 gtgctcgggc tgccggcccg agttgggcga ccgcggcatc tgcagggatt gtccgatctc
     7561 ctgaacgacc cggcgtatgc gacctcggta gggttgctgc ggtggtcgct caaggagcgc
     7621 gagatcatgt tgcgttcctc cccgggcgtg ccgacggcct cgttcggcgg gctcctcaga
     7681 aaggtggcga atctcatgcg cgtggtgttg ccgcaatgac gggcccaagg aacgatggtg
     7741 gcgagcgagg ccaccagcga cagcccactc cgctcgcagc agagactctg ttcgactacc
     7801 tggccgcggc ccgcacggac ggcggccgca gcccggcgcc aggcgcgccc ttcagtcgaa
     7861 gcgaccgcgc cagcgcgacc acctcggggc gtggcacgat caagcaagga gcctccgctc
     7921 cggcagcgac caaggttgcg ggccgcgcgt tagacttgtc ggcggcgccg cctccgccgg
     7981 gaaccgcctc agagtacgac cggggagctc ccccgccttc agctgccgcc atgaatcgtg
     8041 cgggagacag gagctcggac gggtcgggct tcgagttcgg ctatcgcagc gagcccgacg
     8101 gattgccgcc ggaagagcga acagagcgcc cacaacctgt gcgactgcgc gcggtgacgc
     8161 aggggaagac ggttcaaatg gacgaccgcc gtgaggtatc ccttgggcga ccgccgagtg
     8221 aacagcgccc gaataccgcg ttcaacgcgg gcggcgagga cacaccagcc gcaggtgccg
     8281 acatgcccgg cggtgacgag ccgaggagga ccgaggtgac gagtcccctc gagatggaga
     8341 gtgacctgat ggacgacaac ttcgatgcgc tgccgccgat caaggtcgtc ggagtgggag
     8401 gcggcggctc gaacgccgta aaccggatga tctcggcgcg cctcccgggc gtgcagtacg
     8461 tggccctgaa cacggacatg caggcgcttt cgcactgcgg cgccgagatc aaggtgcgca
     8521 tcggcgaccg gctcacgaag ggcctcggcg ccggttcgga gccgctgcgc ggtgcgcggg
     8581 cggctgagga atcgcgtgaa gcgatcgcgg aggcactaca gggcgcggaa atggtgtttg
     8641 tgaccgcgtg cctcggcggc gggaccggca ctggcgccgc accgatcgtt gccgaagtcg
     8701 cgcgcgaact cggagcgctg acaatcggcg tcgtgacgaa gccgtttacg ttcgagggcg
     8761 ccaagcgccg ccagcaagcc gaagagggcg tgcgcgacct gcaggagaag gtcgacacgc
     8821 tcatcgtgat cccgaacgac cgactgcttc agatctgcga cgagaaggtc tcgatgcagg
     8881 acgcgttccg tatcgcggac gacgtgctgc ggcagggcat tcaggggatc agtgagctga
     8941 tcaccgtccc gggcatcgtg aacctcgact tcgcggacgt gcgcaagatc atgtccgacg
     9001 ccggtcccgc gctgatggcg atcggcaccg gacggggtga gcatcgggcg gtggacgccg
     9061 ccaagatggc gatcacgagc ccgctactcg aggtggacat caccggagcg accggggtgc
     9121 tgttcaacgt gaccggcccg gcgaacctcg ggctccacga gttgaacgcg gcggcgcgtg
     9181 tgatcgcgga ggtcgtagac ccggaggccg agatcatctt cggcaccgcg gtcgacgagt
     9241 cgttgcagga cgaggtgcgg atcaccgtca tcggaaccgg gttcacgggc gtccgccccg
     9301 ccgcgatgcg gcctccgcgg gacgagccga tgacggccga cccgaagccg atccggctgg
     9361 ccgacgtgca gcccgagccc agcctcgacc agttgacgga agccgacctg ccgacgttcc
     9421 tccgacggac gttcccgacg cgctagctcc ctctgtgggg atggtacgag aggccctcga
     9481 tcgagggcct ctcgtcgttt cggcggtatc ccgcccgcgt ggacaatttc gacgcgagtt
     9541 cgcacagctc tgtggatggc gcgggttgtg acatgcgggc ctcggtgctt cactgcagtc
     9601 accccactta caccgatgtc ctccccccgc agaggtctgg atccgccgaa tgaaatgccc
     9661 gtactgcggc catatggcca gcaaggtgat cgactcgcgc acctcggacg acggtgtccg
     9721 ccggcgtcgc gaatgccagg cgtgcaacga acgctacacg acgtacgagc agttccagcg
     9781 cagcgtcgtg atggtgatca agaaggacgg ccggcgcgag gagttccagc gcgagaagct
     9841 gctgcacgga ctgcgttcgg cggtgcagaa gcggcctctg ccgacgggct ccatcgacgc
     9901 gatcgtagag agcatcgagc accggctgat gacgtcgggc tggagcgagg tgccgagccg
     9961 cgccattggc gagatggtga tcaagcacct gaaagcgctc gatccgatcg cctacatccg
    10021 tttcgcctcg gtgtaccaca agttcgtgtc cgtcgatgag ctgctgagcg agctcacgca
    10081 gctcgcccag tcgccgccgc cgaccgcaga tcagcctcgg ctgttcgagg acgagttgag
    10141 cgagctcgtg gatggcgacg acagcgcatc gcccgcgccg cctccgccca cgccgatcga
    10201 gcgcgcgcca tcgatcctcg cgaggatgta ggggtgccgc gggagaccct tcgcctggga
    10261 gcggcggcgt aagatcgatc cgcgggcgcg tcaggcccgc ggagggtcca tggtcctgtc
    10321 ggcgacggtc acgacgcatc ccacggcgct caccgagaac gctcgcaccg tcctcgcgcg
    10381 gcggtacctt ctgcgcgacg agagcggaac gatcgtcgag acgccggagc aactgttcgc
    10441 ccgcgtcgcg gaggcgatcg cctcggttga ggcgacggat gaagcgcggc aacgctgggc
    10501 ccagcgcttc tacgacgcga tggcctcgct gcggttcctg ccgaactcgc cgaccctcat
    10561 gaacgcgggg accggtcggg ggacgcttgc agcctgcttc gtgctgccaa tcgaggacac
    10621 actcgagtcc atcatgtcga ccgcgcaggc agcggcgatg gtgcagaagt acggcggagg
    10681 cacgggcttc tcattctcga gactgcgtgg cgaaggcgaa ccaatcgcga ccacgcatgg
    10741 tgcggcctgc gggccggtgt ccgtattgcg ccattacgac gacgtctcac gcctcgtgac
    10801 gcagggcggg aagcgcgagg gcgccaacat gggcgtactg cgtgtggatc acccggacat
    10861 tcgagccttc atccacgcga aggatgacgg ggtatcggcg cagcgcttca acctctccat
    10921 cgccgtcact gacgaattca tgcgtgcggc cgcgaccggc ggctcgctcg agctacgcga
    10981 cccgcgggac ggatcggaac gagggagcga agacgcggcc gcgctgctgt cggacatcgc
    11041 ccgctcggcg tgggcgaccg gtgacccggg gctcgtgttc ctcgacacga tcaaccgcga
    11101 caacccgacc cctcagatcg gcgccatcga ggccacgaac ccgtgcggcg aggttccgct
    11161 gttgccgtgg gaggcgtgca cactcggatc gatcaacgtc gcgcggttct gggacgccga
    11221 ggctggcgac ctggattggg acacactcgg cgagaccgtg acgctcggcg tgcggttcct
    11281 cgatgacgtc gtcgaggcga acacctttcc gtcaaaggag atcgctgccg cggtacgcgg
    11341 gaaccgaaag atcggcctcg gcgtcatggg cttcgcggat ctgctgatca ctgcggggct
    11401 gccgtacgac tcggaggagg cgctcgtact ggcaaaccgg cttgggcggt tcatctcggg
    11461 ccaggcggac gaggcctccg cgg
//
LOCUS       DAORPG010000003        11194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_209_length_11194_cov_3.581381, whole genome shotgun sequence.
ACCESSION   DAORPG010000003 DAORPG010000000
VERSION     DAORPG010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 11194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11194
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209_length_11194_cov_3.581381"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLX85_00180"
     CDS             <1..270
                     /locus_tag="PLX85_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61623.1"
                     /translation="LIPVAAPGKLLGAAPMDSQPQGVPMASKKARVLVDALIDGKECK
                     VNDVITLEDDQIKAYAGQVDPHPDAVKHAEKLAAAAAEAAKQAKG"
     gene            274..885
                     /locus_tag="PLX85_00185"
     CDS             274..885
                     /locus_tag="PLX85_00185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HRC61624.1"
                     /translation="MTGKQLVTGPSIEPVSLNEMKAHLRVFGSEEDGLLAGYILSARR
                     HIEIETGRALISQRWRATFDGGWPVAFDGVGFRTRVVLPVAPLLAIQSVQYIDSAGAT
                     QTLSSGLYRVARVADRRQRGFIEPAHGASWPAVQSVAEAVSVTFDAGYGTGPGDVEGE
                     ITQAILLLAAHWYENREAVVIGTTVSEMPLAVERLISRYRLDI"
     gene            895..1401
                     /locus_tag="PLX85_00190"
     CDS             895..1401
                     /locus_tag="PLX85_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61625.1"
                     /translation="MPQNANITINLEGLLQAISALGTAEYKLAAGYVNRLLEGVAAGQ
                     IDRWYHAQRTIAASGNEDLDFSGSLVNPLNATAVFARLKVLIVSAAAGNTNNVNVTRP
                     AANGVPWALAASDGLAVAPGETKVLINRSDATGIVVTAGTGDLINFANSGAGTGVTYD
                     IFALGCSA"
     gene            1401..1754
                     /locus_tag="PLX85_00195"
     CDS             1401..1754
                     /locus_tag="PLX85_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HRC61626.1"
                     /translation="MRAGRLRNRVVIQRRTGAVNAFNEPTDTWSALATVWAGIEPISG
                     REYFAAQQVQSELTHRVTIRYLEGVTPKDRVAWTDPATQATRYFDIRAVIDRDERHRE
                     QQLMCVEHAGSFTSN"
     gene            1729..2196
                     /locus_tag="PLX85_00200"
     CDS             1729..2196
                     /locus_tag="PLX85_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HRC61627.1"
                     /translation="MLEASLRIEGLDRVVANLERLQPIAREKAMRGAMFKAMRPVLLA
                     IQATTPRRSGALRLAMARVYLRPKQGLTDGLAARVAVAPKTKNRQALALANFSYARTR
                     RLRGVYWGHYVEFGTRRGIKPGRFMQRALAANAQPVIDIFAGDIDAQIRRQLK"
     gene            2193..2579
                     /locus_tag="PLX85_00205"
     CDS             2193..2579
                     /locus_tag="PLX85_00205"
                     /inference="COORDINATES: protein motif:HMM:NF022810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HRC61628.1"
                     /translation="MIDKDLVAFLKARQTGAGTRVHSSLLPQETAYPAVAVTRISGSV
                     DRGLGGEVILGRGTWRIDVYGRDFESMFGVSDEIRDALDGFRGLMGTTSIKACQLQSF
                     SDFSEEDGERKLRHLVHDFSIVHSED"
     gene            2583..3035
                     /locus_tag="PLX85_00210"
     CDS             2583..3035
                     /locus_tag="PLX85_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61629.1"
                     /translation="MGTSTGAGGLFRIGLAVNAAPESVPAYASDTLRNVGYIESDTPP
                     PFKKKISEVTTLADGTLTFGGGLETQTYEATVVRNFGDTPHEDLFTDGRALLNQYRNF
                     EIVFSDSGAEAWRFKGFVSEYSPQERTSENAFKVKLTIQVYGNITVVP"
     gene            3032..3409
                     /locus_tag="PLX85_00215"
     CDS             3032..3409
                     /locus_tag="PLX85_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61630.1"
                     /translation="MTDPRQVFEGLRKVRNPEPFDLENGQRIHIKGLNTFERMCFADY
                     VAAMKEAETGAQEMSFRIAAFVTHLGVVNTSGERVFESIEDVVKVFGDIEGDGLAMVF
                     SVAQAILDRSGLAARSGESAEKN"
     gene            3487..3738
                     /locus_tag="PLX85_00220"
     CDS             3487..3738
                     /locus_tag="PLX85_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61631.1"
                     /translation="MSSEEFTDWQAAYRIAPWGEHLADVHFAQLNATVCNASGNFKSP
                     RETRSFLLLKDPSPKTSLSASARKYLKRPKRGNRRNSHR"
     gene            3710..5422
                     /locus_tag="PLX85_00225"
     CDS             3710..5422
                     /locus_tag="PLX85_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61632.1"
                     /translation="MATAGTVTVDFDARSAKFAAELEKVQSSLRKLEGSTAVVSKAFQ
                     ALPGILSAGTIIAYGKAMFEAADAIGDAAARSGIAAESLSRLKFAAEQSDVEFGALTT
                     GIKAFQRNLSEATSGSDTASRSFLAIGLEASKIRNLRLEDQLATIADGFKRLSSPADK
                     TRVAMELFGKAGNELIPFLDQGSAGIQKLTAEADRLGITLNNQTVAAVSAADQALKKL
                     KATVNSFFQRALGDIALAIVGIDGLSEIDQLTVKVKRLADEKQRIELSGAPAGSGFAA
                     RLKEINAELAIMQPKLQNLQTAASLTLPASEPGQGRAGGGGGANAQGGVGGLFKPPSK
                     EELDRLGGVSLGPLFSPEQLAEQQQIIADQTSLARQQDWQEFLQYEFLKRDAVDETYK
                     AQLAMAQVASETEIQMRQATVNAALGFLNTLAVRSKAAAIAAVLLSRGLAIAQAVQNT
                     AVAVTKALAVDPTGALASRVALLGKIQIGIIAATGALEVANITSGNAGGIGGTSLGTP
                     SNPLPTTSADAQGATPNRSITVVFNGPVTEREALLGIIQDAFDADVVVIPGNSRQAQE
                     IRGG"
     gene            5422..5832
                     /locus_tag="PLX85_00230"
     CDS             5422..5832
                     /locus_tag="PLX85_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61633.1"
                     /translation="MAYVAYTARRSLAPGHIENFSYTLPLRFTRADRRTDANVHPKRS
                     LSGKTETLFHGLVVTWDVGVAPVAQPLVDILREFLDSTLDGQTFQFNPFSDVESDEIS
                     VIREDDGYPDRRFLQTGLGHRFDRFAFDFRLREV"
     gene            5829..10352
                     /locus_tag="PLX85_00235"
     CDS             5829..10352
                     /locus_tag="PLX85_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61634.1"
                     /translation="MKYFSPKFSQADRASDRRDRLIVNIVFDVGSLYFTSHDDITGVP
                     GTVLNGVVRDVSAVSQRIVPDEGRSEIGSMSFSLVDRNSTVTNEFRSKLNSAKGLRGK
                     TVRLYRGFEGFTWSEFSLFQTQVIRDAEFDFGAYSIQCSDITREQRKEIFQPVSTGLR
                     LSCSETDTTIAVYDTAKFLGVEHGASYSDAPGQTVVYFKIQDETIRCPVSGIGPDTFT
                     GCQRGALNTRPRAHAVDATTAADQRTKVEEVVYLELPAGMLAFALLTGVLHGQSGTLP
                     AHWHLGIDPSLVKLSDFTGIGPDLWKTSDHTQALILRFAGEKAQDGKAFLEKKVLQPM
                     GCFSPVYADGTLGLRRMAALTSDAAAVATLTEDHFVSHSGLQHQMSRMINVFQIDWSY
                     EGKEPRRQSRFTDLASQAVHGKAPLKKLEWPGIYGSRHTDITIWQRLYALRDRYSAPP
                     LGLDGSLVPSLASLEVGDVVRVKVANLRNYTGAGDFIDQAMEIQQISINHTTGDLDVD
                     LFGSTARPSTTPPADLSSFPLPDGFYSAAGTLLSAIAGVTVAGNVLTVAPGTPYNGGA
                     DLTASQAIVYWLGDLTIPNGVTLRIAGNVWLRVMGDLVINGTIDGNYGGLPGVADDGA
                     LTPYLLGNPGFIGNSRGLDGLRVTEMGGNPQLESQPIPTTEGRFAAFPFLTLQISGNN
                     LLGVPTDLRGSGGGPGGKVVYQSTLLKNGGTGGAGGAGLCITARRIDIGASGQVTLRG
                     ADTAAPTPHLEQGKNFYPGTGGAGAPGSLLLLLDAGPFVTAPDLTGKFYGVSGAVAQP
                     YYSPEFPVFFMPSRAHQRRSRSEQPYAGYLGPEIISAQDLSNACHRIQYIPPVVLPVE
                     DVDDPPPPPIAVTAIPGPGYNLVTIFPAEISGATVELYESLDGNVANATFLQDLAGTQ
                     FYHALLGEETRYYHARSKLELDANTVLTSDWFAGTVVSTAGTAIAADLPEERDFLDTY
                     EHQDALGKYDSVYGSGTLTYPTDGSYGGHVLQVAGGSRSLIWKQNIPLDPTALYRMSV
                     RVRQTMASTNSTKDGVWVGFRAIGADGVTPIQLGDPGDHYVAAANFDMGSVPLGTWAT
                     LIGYFSSQTNIKLSPAPDIDNPSGVSFGTAYLRPIVFANYFDGDGTQQFDYVRIEKLI
                     DGRWQDIDGEGKPSDYADNTFSQNPGATGVFYDNFEHQDIWRFYTSKSAAVAGVSYPL
                     DGIDGGRVLQVQGDLWAEWKENIPLDPNAIYRLTGRVRRTVAGAAGAERLVVGYQGIA
                     ADGVSYINTLGANLDAAQHWQIYFFDMGTIGVGTWTDFVCWASGNGTPVVLGNGSPGA
                     PMKFYPGVKYFRPTFWVNNPDGTGTVQVDRIRIERLIPLATGLIANGAVFEPFSATTA
                     SSDVYNSGSTSTQVVQVTVPAQDVPTTQVVTVVGNVDHNAASGTQGSTFALITDAFSS
                     GQSYATEDTIARNPSTSLLRQIQTFSLERSYSVAAGVAKTFSLSALGMGFSAAGSSCL
                     LKSVRILVKVFKA"
     gene            10349..10762
                     /locus_tag="PLX85_00240"
     CDS             10349..10762
                     /locus_tag="PLX85_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61635.1"
                     /translation="MSRTWSFYDPATGEFTGRTYSGSPAGLELNTPDGCSAFEGSVDH
                     ETMRVNPETDALEQWRDPPPSADHVWDAAGQAWRLSRTAQQRDVLARIGELEAQQLRP
                     LRELALDPASAQARSRVQAIETEISLLRALLTRPD"
     gene            10778..>11194
                     /locus_tag="PLX85_00245"
     CDS             10778..>11194
                     /locus_tag="PLX85_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61636.1"
                     /translation="MTDYRVLARTAAARLNALIARFLPHAKPVRYLLVVFVAALVTAL
                     SVKCRAGTIMFEAGSTVIRGETPSIGLYMQTPRADRGGDYNYECGLGLVGSTTAEGRA
                     WLKLRDEALQALDRAEEARRAHQEIDSDRLAMIESVQ"
BASE COUNT         1945 a         3761 c         3625 g         1863 t
ORIGIN      
        1 ctgatcccgg tcgcggcgcc cggcaagctg ctgggcgccg cgccgatgga ttcccaacct
       61 caaggagttc ccatggcttc caagaaagcg cgcgtcctcg tggatgcgct gatcgatggc
      121 aaggagtgca aggtcaacga cgtgatcaca ctcgaagacg accagatcaa ggcctatgcc
      181 ggacaggtcg atccgcaccc ggacgcggtg aagcacgccg agaagctcgc ggccgctgcg
      241 gcggaagcgg cgaagcaggc gaaaggctga tcgatgaccg gcaagcagct cgtgaccggc
      301 ccgtccattg agccggtctc tctcaacgag atgaaggcgc acttgcgcgt cttcggctcc
      361 gaggaggatg ggttgcttgc cggctacatc ctgagcgcgc ggcggcacat cgagatcgag
      421 accggccgtg ccctgatctc acagcggtgg cgggctacgt tcgacggagg gtggccggtc
      481 gcgttcgacg gagtggggtt tcgcacgcgg gtcgtgctcc cagtggctcc attgctcgcg
      541 atccagtcgg tgcaatacat cgactcggcc ggggcgaccc aaacgctatc gagcgggctg
      601 taccgagtcg ctcgagtggc cgatcggcgg cagcggggct tcatcgagcc ggcgcatggt
      661 gcgtcttggc cggcggttca gtcggtggcc gaggcagtgt cggtcacgtt cgacgcgggc
      721 tacggcactg gccctggcga cgtcgaaggc gagattaccc aggccatcct cctgctcgcc
      781 gcccactggt atgaaaaccg cgaggccgtc gtcatcggga cgacggtctc agaaatgccg
      841 ctcgcggtag agcggctgat ttcccgttac cgactcgata tctaggagtg ttccatgccg
      901 cagaacgcaa acatcacgat taacctcgag ggcctgctgc aggccatctc ggcgctcggt
      961 acggcggagt acaagctcgc cgcgggctac gtcaatcggc tgctggaggg cgtggccgcc
     1021 ggccagatcg atcgctggta ccacgcgcag cgaacgatcg ccgcgagcgg caacgaggac
     1081 ctggacttct ccggttccct ggtcaatccc ctgaacgcga cggccgtctt cgcgcgcctg
     1141 aaggtgctga tcgtctcagc cgcggcgggg aacacgaaca acgtgaatgt cacgcgaccg
     1201 gcggccaacg gcgtgccctg ggctctcgcc gcgagtgatg gcctggcggt ggcccccggg
     1261 gagacgaaag tgctgatcaa tcgatcggac gcgaccggca tcgtggtgac cgccggaacc
     1321 ggcgatctga tcaacttcgc gaactccggc gccggaacgg gagtgaccta cgacatcttc
     1381 gcgctgggct gctcggcctg atgcgagccg gccggcttcg gaatcgcgtc gtcatccagc
     1441 ggcgcactgg cgccgtcaac gcgttcaacg agccgaccga tacctggtcc gcccttgcca
     1501 ctgtctgggc ggggatcgaa cccatcagtg gtcgcgaata cttcgccgcg cagcaggtgc
     1561 agagcgagct gacgcatcgc gtcacgattc gctacctcga gggcgtcacg ccgaaggatc
     1621 gcgtcgcgtg gaccgatccc gccacgcagg ccacgcggta cttcgacatc cgcgcggtga
     1681 tcgatcggga cgagcggcac cgcgagcaac agctcatgtg cgtggaacat gctggaagct
     1741 tcacttcgaa ttgaaggcct ggatcgcgtc gtcgcgaacc tcgagcggct gcagccgatt
     1801 gcccgcgaga aggcgatgcg cggggccatg ttcaaggcca tgcggccggt gctcctcgcc
     1861 atccaggcga cgacgccgcg gcgctcaggg gccctcaggc tcgccatggc ccgcgtctac
     1921 ctgagaccga aacaggggct gacggatggg ctcgctgcgc gtgttgcggt ggcgccgaag
     1981 acgaagaacc gccaggcgct ggcgcttgcg aacttcagct acgcccggac gcggcgcctg
     2041 cgcggcgtct actggggcca ctacgtggag ttcggcactc gccgcggcat caagcctggg
     2101 cggttcatgc agcgcgcgct tgccgcgaat gcgcagccgg tgatcgacat cttcgccggc
     2161 gacatcgacg cgcagatccg gaggcagttg aagtgatcga taaggacttg gtggccttcc
     2221 tcaaggcacg gcagacggga gctggcactc gcgtgcactc gtctctgctg ccgcaggaga
     2281 ccgcctatcc ggctgtcgcc gtcacgcgga tttcgggctc cgtcgatcgc ggcctcggtg
     2341 gcgaagtcat cctggggcgt ggcacctggc gcatcgacgt ctatgggcgc gacttcgaat
     2401 cgatgttcgg cgtctcggac gagatccgcg atgcgctcga cggcttccgg ggcctcatgg
     2461 ggacgacgtc gatcaaggcc tgccaactgc agagtttctc ggacttctcg gaagaagacg
     2521 gggagcgaaa gctccgtcat ctcgttcacg acttcagcat cgttcactcg gaggattgat
     2581 ctatgggcac ttccaccggc gccggcggcc tgttccgcat cggtctcgcc gtcaacgcgg
     2641 ctcccgagtc ggtgccggcc tacgcttcgg acaccctgcg caacgtcggc tacatcgaat
     2701 cggacacccc tccgccgttc aagaagaaga tcagcgaggt caccacgctc gccgatggca
     2761 ccctgacctt cggcggtggg ctggagacgc agacctacga agcgacggtc gttcgcaact
     2821 tcggtgacac gccccacgag gatctgttca ccgatggtcg cgcgctgctc aatcagtacc
     2881 ggaacttcga gatcgtcttc tccgattccg gggccgaggc ctggcgcttc aaggggttcg
     2941 tctccgagta ctcgccccag gagcgcacca gtgagaacgc cttcaaggtg aagctcacca
     3001 tccaggtgta cggcaacatc accgtcgtgc cgtgaccgat ccgcgccaag tattcgaggg
     3061 gctccgtaag gtccgcaacc cggagccctt cgatctcgag aacggccagc ggattcacat
     3121 caaggggctc aataccttcg agcgcatgtg cttcgccgac tatgtcgcag cgatgaagga
     3181 ggcggagacc ggggctcagg agatgagctt ccgcatcgcc gcgttcgtca cgcacctcgg
     3241 cgtggtcaac acgagcggcg agagggtctt cgaatcgatc gaggatgtgg tcaaggtctt
     3301 cggggacatc gaaggcgatg gcctcgcgat ggtgttctcc gtcgctcagg cgatcctcga
     3361 tcggtccggg ctcgctgcgc gcagcgggga gagtgccgaa aaaaactgag atcgcggccg
     3421 gagcggctgt tcgcgttctt tctggcaagg acgctgcact gcacggtcgc ggaactcggt
     3481 gagcgagtga gctccgagga gttcacggat tggcaagcgg cctatcgcat tgcgccttgg
     3541 ggagagcatc tggcggatgt ccacttcgcc cagctcaacg ccaccgtctg caatgcgtcc
     3601 ggcaacttca agtccccgag ggagacgcgg agcttcctgc tgctgaagga cccctcgccg
     3661 aagacatccc tttccgccag cgcgcgcaag tacctcaaga ggccgaaacg tggcaaccgc
     3721 cggaacagtc accgttgact tcgatgcgcg ctccgcgaag ttcgcggccg aactggagaa
     3781 ggtgcagtcg agccttcgca agctcgaggg cagcacggcg gtggtatcca aggccttcca
     3841 ggcgcttccc ggtattctct ctgccgggac gatcattgcc tacggaaagg ccatgttcga
     3901 ggcggccgac gccatcggcg atgcggcggc ccgcagcggc atcgcggccg agtccctgtc
     3961 tcgcctgaag ttcgccgctg agcagtcgga cgttgagttc ggcgcgctga cgaccgggat
     4021 caaggcgttc cagcgcaacc tctctgaggc cacatccggc agcgatacgg cgtcgcgctc
     4081 gttcctcgcc atcggtcttg aggcatcgaa gatccgcaat cttcgcctcg aggaccagct
     4141 cgcgaccatc gctgatggct tcaagcgcct gtcgagcccc gcggacaaga cccgcgtcgc
     4201 catggagctc ttcggcaagg ccggaaacga actcattcct ttccttgacc agggctcggc
     4261 gggaatccag aaactcaccg cagaagccga tcgcctcggc atcacgctga acaatcagac
     4321 cgtggcggcc gtcagtgcgg cggaccaggc gttgaagaag ctgaaggcca cggtgaacag
     4381 cttctttcag agggcgctcg gcgacatcgc gcttgcgatc gttggcatcg atggtctcag
     4441 cgagatcgac cagctcaccg tcaaggtgaa gcggctcgcc gatgagaagc agcggatcga
     4501 actctcggga gcgccggccg gcagtggttt cgcggcgagg ctcaaggaga tcaatgcgga
     4561 attggcgatc atgcagccca agctgcagaa cctccagacg gcagcatcac tcacgctgcc
     4621 cgcatcggag ccggggcagg gccgcgccgg cggtggtggc ggcgcgaacg cgcaaggcgg
     4681 cgtgggcgga ctattcaagc caccttcgaa ggaggaactg gatcgcctcg gaggggtgag
     4741 cctcgggcct ctcttcagtc cggagcagct cgctgagcag cagcagatca tcgccgatca
     4801 gacgagtctg gcgcgccagc aggactggca ggagttcctg cagtacgagt ttctgaagcg
     4861 ggacgccgtc gacgagacct ataaggccca gctcgccatg gcgcaggtcg ccagcgagac
     4921 cgagatccag atgcgccagg cgacggtcaa tgcggcgctc ggtttcctca acaccctggc
     4981 ggtccgttcc aaggccgctg cgatcgctgc ggtgcttctg agtcgcggcc tcgcgattgc
     5041 ccaggctgtt cagaacaccg ccgtcgcggt aacgaaagca ctcgccgtcg atcccacggg
     5101 cgcgcttgcc agccgcgtcg cgctgctcgg caagatccaa atcggcatca ttgctgcgac
     5161 gggtgcgctg gaggttgcga acatcaccag cggcaacgct ggcggaatcg gcggcacgtc
     5221 cctcggaacg ccctccaacc cgctgccgac gacgagcgca gacgcgcagg gcgcgactcc
     5281 gaaccggagc atcaccgtcg tgttcaatgg cccggtcacg gaacgtgagg ccctgctggg
     5341 aatcatccag gacgccttcg acgcagacgt cgtcgtgatc cccggcaaca gtcgtcaggc
     5401 ccaagagatc aggggcggct gatggcctac gtcgcctata cagcccggcg ctccctcgct
     5461 cccggccaca tcgagaattt ctcttacacc ctgccgctcc gcttcacgcg cgccgaccga
     5521 cgcactgatg cgaatgtgca tcccaagcgc tcgctctcag gcaagaccga gacgctgttc
     5581 cacgggctcg tcgttacatg ggacgtcggc gttgcgcctg ttgcccagcc cctcgtcgac
     5641 attctgcgcg agttcctcga cagcaccctc gatgggcaga ccttccagtt caatcccttc
     5701 agcgacgtcg aatccgacga gatcagcgtg attcgagagg acgacggcta tccggatcgg
     5761 cgctttctgc agacgggact cggccaccgc ttcgatcgct tcgcattcga tttccgactg
     5821 cgtgaagtgt gaagtacttc agtccgaagt tctcgcaggc ggatcgcgca agtgaccgcc
     5881 gggaccggct catcgtcaac atcgtcttcg acgtcggttc gctctacttc acgagccacg
     5941 acgacatcac cggcgttccc gggaccgtgc tcaacggcgt cgtccgcgac gtgtcagcgg
     6001 tctctcagcg catcgttccg gacgagggtc ggtcagagat cggctccatg tccttcagcc
     6061 tggtcgatcg caacagcacg gtcaccaacg agtttcgtag caagctcaac agcgcgaagg
     6121 gtctgcgcgg caagaccgtg cgcctctacc gagggtttga gggcttcacc tggtcggagt
     6181 tctcgctctt ccagacgcag gtcatccgag acgcggaatt cgacttcggt gcctactcga
     6241 tccagtgctc cgacatcacg cgggagcagc gcaaggagat cttccagccg gtctccaccg
     6301 ggctgcgact ctcctgcagc gagaccgata ccacgatcgc ggtgtatgac accgccaagt
     6361 tcctcggagt agagcacggc gcgtcctatt cggacgctcc gggccagacc gtcgtctact
     6421 tcaagatcca ggacgagacc atccgctgcc cggtgtcggg gatcggtccg gatacgttca
     6481 ccggttgtca gcgtggtgcg ctgaacacgc ggccgcgggc gcatgccgtc gatgcgacga
     6541 cggcagccga tcagcgcacc aaggtcgagg aggtggtcta cctcgagctg cccgcgggga
     6601 tgctcgcctt cgcgctgctg accggggtgc tgcacggcca atccgggacc ctgccggcgc
     6661 actggcacct gggcatcgat ccgtcgctgg tgaaactcag cgacttcacg gggatcggcc
     6721 ctgacctctg gaagacgagc gatcacacgc aggcgttgat cctgcgcttc gccggcgaga
     6781 aggcacagga tggcaaggcc ttccttgaga agaaggtcct ccagccgatg gggtgtttct
     6841 cgccggtgta cgccgacggc accctcggcc tgcgccgcat ggcggcgctg acctcggatg
     6901 ccgcagcggt tgcaaccctc acggaggacc acttcgtcag tcattccggg ctgcagcatc
     6961 agatgtcccg gatgatcaat gtattccaga tcgactggag ctacgagggg aaggagccgc
     7021 ggcgtcaatc tcgcttcacg gacttggcct cccaggcggt gcatgggaag gcgcccctga
     7081 agaagctcga atggccaggc atctacggct cgcgccatac agacatcacg atctggcagc
     7141 ggctctacgc cctgcgcgat cggtacagtg cgccgccgct ggggctcgac ggaagcctgg
     7201 tgccatcgct cgcgtcgctc gaggtgggcg acgtcgttcg tgtgaaggtg gcgaacctgc
     7261 gcaactacac cggcgccggg gacttcatcg accaggcgat ggagatccag cagatctcca
     7321 tcaaccacac gaccggcgat ctggacgtcg atctgttcgg ttcaacggcc cgtccgtcga
     7381 ccacgcctcc ggcggacctc tcgagcttcc ctctgccgga tgggttctac tccgcggcgg
     7441 gcacgttgct ctcggcgatc gccggcgtca cggtcgcggg caatgtgctc acggtggcgc
     7501 cagggacgcc ctacaacggc ggcgcggacc tcactgcatc gcaggccatc gtctactggc
     7561 tcggcgacct gacgatcccc aacggcgtca cgctgcgcat cgccggcaat gtctggctgc
     7621 gggtgatggg cgacctcgtc atcaacggca cgatcgacgg caactatggc gggttgccgg
     7681 gggtggccga cgatggcgcg ctgacgccat acctgctcgg gaatccggga ttcatcggca
     7741 acagccgcgg cctcgatggg ctgcgcgtca ccgagatggg cggcaacccg cagctcgagt
     7801 cgcagccgat tccgacgacc gagggccggt tcgcggcatt cccgttcctg accctgcaga
     7861 tcagcggcaa caacctgctg ggcgttccga ccgatctgcg cggatcaggg ggcggacccg
     7921 gcggcaaggt tgtttatcag agcaccctgc tcaagaacgg tggtaccggc ggcgcgggcg
     7981 gtgcgggact gtgcatcaca gcccggcgca tcgacatcgg cgcctccggc caggtcacgc
     8041 tgcgcggcgc cgataccgcg gcccccacgc cgcatctcga gcagggcaag aacttctatc
     8101 cgggcaccgg cggagccggc gccccgggct cgctcctgct gctgctcgat gccggcccgt
     8161 tcgtgacggc ccccgacctc acgggcaagt tctacggcgt gtcgggcgcg gtggcgcagc
     8221 cgtactactc gcccgaattt cccgtcttct tcatgccctc gcgcgcgcac cagcggcgtt
     8281 cgcgctcgga gcagccgtac gccgggtatc ttggtcccga gatcatcagc gcgcaggacc
     8341 tgtcgaacgc ctgccaccgg atccagtaca tcccgccggt ggtcctgccg gtcgaggacg
     8401 tcgacgatcc gcccccgccg cccatcgccg tcaccgcgat tccaggcccc ggctacaacc
     8461 tggtcacgat cttcccggcc gagataagcg gggccacggt ggaactgtac gagagcctgg
     8521 acggcaacgt ggcgaacgcc acgtttctgc aggatctcgc cggcacccag ttctatcacg
     8581 cgctcctggg tgaggagacc cgctactacc acgcgcgctc gaagctcgag ctggacgcga
     8641 acacagtcct cacgtccgac tggttcgccg gcaccgtggt gtcgaccgcc ggcaccgcga
     8701 ttgccgcgga cctgccggag gagcgggact tcctcgacac ctacgagcac caggacgcgc
     8761 tcggcaagta cgactcggtc tacggctccg gcacgctgac ctatccgacc gacggctcct
     8821 acggcggcca cgtgctgcag gtggcgggtg gctcccgctc cctcatctgg aagcagaaca
     8881 tcccgctcga cccgacggcg ctgtaccgca tgtcggtgcg cgtgcgtcag acgatggctt
     8941 cgacgaactc caccaaggat ggcgtctggg tgggcttccg cgcgatcggg gcggatggcg
     9001 tcacgccgat ccagctcggc gacccgggcg atcactacgt cgccgcggcg aacttcgaca
     9061 tgggttcggt gccgctcggc acctgggcga cgctcatcgg gtacttctcg agccagacga
     9121 acatcaagct cagccccgcg ccggacatcg acaacccgtc cggcgtctcc ttcggtaccg
     9181 cctacctgcg gccgatcgtc ttcgcgaact acttcgatgg cgatggcacg cagcagttcg
     9241 actacgtgcg catcgagaaa ctcatcgatg gccgctggca ggacatcgac ggggagggaa
     9301 agccgagcga ctacgccgac aataccttca gtcagaaccc gggtgcgacg ggcgtcttct
     9361 atgacaactt cgagcaccag gacatctggc gcttctacac gtcgaagagc gcggccgtcg
     9421 ccggtgtctc ctacccgctc gatggcatcg acggcggtcg cgtcctgcag gtccagggcg
     9481 atctctgggc cgagtggaag gagaacatcc cgctcgaccc gaacgcgatc tatcgcctga
     9541 cgggccgcgt ccggcgcacg gtggcgggcg ctgcgggagc cgagcgcctg gtggtgggct
     9601 accagggcat cgcggcggac ggcgtctcct acatcaatac cctgggcgcc aacctcgatg
     9661 ccgcccagca ctggcagatc tacttcttcg acatggggac gatcggcgtc ggcacctgga
     9721 ccgacttcgt ctgctgggcc tcgggcaatg gcacgccagt ggtgctgggc aacggctcgc
     9781 cgggcgctcc gatgaagttc tacccggggg tgaagtactt ccgtcccacg ttctgggtca
     9841 acaaccccga cggcacgggt accgtccagg tcgaccggat ccgcatcgag cgcctgatcc
     9901 ccctcgccac gggcctgatc gcgaacggtg cggtattcga accgttctcc gcgacgaccg
     9961 cgtcctcgga cgtctacaac tcgggcagca ccagcaccca ggtcgtccag gtgacggtac
    10021 ccgcccagga cgtcccgacg acccaggtgg tcacggtcgt cggcaacgtc gatcacaacg
    10081 ccgcgagtgg cacgcagggc agcacgttcg ccctcatcac ggacgccttc tcgtcgggcc
    10141 agtcctatgc gaccgaggac acgatcgcgc gcaacccgag cacctcgttg ctgaggcaga
    10201 tccagacgtt ctcgctcgag cgcagctaca gcgtggcggc gggtgtggcc aaaaccttct
    10261 cgctgtcggc cctcggcatg ggcttctccg cggccggctc gtcgtgcctc ctgaagtcgg
    10321 tgcggatcct cgtgaaggtc ttcaaggcat gagccgcacc tggtccttct acgaccccgc
    10381 gacgggcgag ttcacgggcc gcacgtactc ggggtcgcca gccggcctcg agctgaacac
    10441 ccccgacggc tgtagcgcct tcgaggggtc ggtcgaccac gagacgatgc gcgtcaaccc
    10501 cgagaccgac gcgctcgagc agtggcggga cccgccgcct tccgccgatc acgtctggga
    10561 tgccgccggc caggcctggc gcctctcgcg aacggctcag cagcgggacg tgctcgcgcg
    10621 gatcggcgaa ctcgaagcgc agcagctccg cccgctacgc gagctcgcgc tcgatcccgc
    10681 ctcggcgcag gcccgctcgc gggtccaggc gatcgagacc gagatctcgc ttctgcgcgc
    10741 ccttctgacg cgccccgact aacaaggaga accgacgatg accgactatc gcgtcctcgc
    10801 gaggaccgcg gctgcccgcc tcaatgccct gatcgcgcgc ttccttcctc acgcgaagcc
    10861 ggtgcgctac ctgctggtcg tgttcgtcgc ggccctcgtc accgcgctct cggtcaagtg
    10921 tcgcgcgggc accatcatgt tcgaggccgg atcgaccgtg atccgcggcg agacgccgag
    10981 catcggcctc tacatgcaga cgcccagggc agaccgcggc ggcgactaca actacgagtg
    11041 cggcctcggc ctggtcggct cgacaacggc cgaggggcga gcctggctca agctccggga
    11101 cgaagccctc caggcgctcg accgggcgga agaagcccga cgtgcccacc aggagatcga
    11161 ctccgatcgc ctggcgatga tcgaatccgt ccag
//
LOCUS       DAORPG010000004         9335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_275_length_9335_cov_3.599138, whole genome shotgun sequence.
ACCESSION   DAORPG010000004 DAORPG010000000
VERSION     DAORPG010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 9335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9335
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_275_length_9335_cov_3.599138"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1256)
                     /locus_tag="PLX85_00250"
     CDS             complement(15..1256)
                     /locus_tag="PLX85_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HRC61637.1"
                     /translation="MSKLNALREERGSLAKVVKALVEDTSKKWGPDQQKVYDEKIARI
                     DDIDAEIGREQKVLDLMAAESRVIETIAETGGLSLEQAKARNEREREIFRKWARGGDQ
                     AVSNEEWKLLRSLRNTMSTTTAAEGGYTVPTTVAASVFEALKEFGGMRDAADVFATSS
                     GEQINYPTTDGTAEVGELIGENTTATGLDPSFGVVSLSTFKYSSKIVAVPYELLQDSN
                     IDVEALIRGRLGERLGRITNQHYTTGTGSGQPRGVVTGSTVGKTGTTGQTTTVIYDDL
                     IDLEHSVDPAYRRRGAGYMMADSSVKVIRKIKDTQGRPIFVPGYEVGSPGGAPDTLLG
                     RPIIVNNDVAAMAANAKSILFGLFKNYKIRDVMAMSLFRFTDSAYTKLGQVGFLAWMR
                     TGGQLVDVSGTSVRHYANSAT"
     gene            complement(1341..2051)
                     /locus_tag="PLX85_00255"
     CDS             complement(1341..2051)
                     /locus_tag="PLX85_00255"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HRC61638.1"
                     /translation="MNRIQSRLQILRNRQTPALPTPQAKSDAEEATLYLYDVIDPFWG
                     VSAAGFVKELQGIQAKTIHLRINSPGGDVFDGRAIATAIRQHPAKIIAHVDGMALSAA
                     SWIALAAAEVEIADGAYFMIHRAWAFAAGNAADMLSMAELLEKVDQDLIREYVAETGN
                     VEDKIRAWLDAETWFNADEAVDNGFADRKVEAFAEAKNAWDLSVYDKTPQALLESAAP
                     AIDWEAKHLEASRFARAL"
     gene            complement(1972..3294)
                     /locus_tag="PLX85_00260"
     CDS             complement(1972..3294)
                     /locus_tag="PLX85_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HRC61639.1"
                     /translation="MSLEQVIGALNRWSPRNDDIVIQPPAITWENWDDFFGMHGTVAG
                     VRVTAANAMNVAAVYACVRILAGVIGSLPLPVFERAGDERRAVKHPLWWLLNEQANER
                     MTTVSFWEYVLACMFLYGDGFAVIRRNSNNIVQAFVPVDARAVGIKTIRTSRWRYETV
                     YFVADGAESYGVPFEDMLHFSSLGFNGERSLSVIQSAAMNAIGIAMAADQHSANTLAA
                     GLTSRVVLESPTGVRHDDEQLEQMRRMFIERYGASPTGGPNSALPIILSNGMTAKPLT
                     INPVDAQLLESRKFQVVDIARAFGVPPFMIGETEKSTSWGSGLEQQVLGFVKFTLNPH
                     LRRIEQELNRKIWPVRERYFTEFNVNGLLRGDAKSRSDFYRAALGGAQGDGWMSIDEV
                     RKLENLPPNENADALYRAPRKADEPNPVPATDPAEPADPGAPDPASEE"
     gene            complement(3294..4970)
                     /locus_tag="PLX85_00265"
     CDS             complement(3294..4970)
                     /locus_tag="PLX85_00265"
                     /inference="COORDINATES: protein motif:HMM:NF015321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HRC61640.1"
                     /translation="MAKPKSTADVVRAYCDGVMSGKIAAGEWHRKAVARHIDDLKHGH
                     KRGLRFDPKRAAYVIEFFGFLVQFEGEWANQPFHLEPWQMFIVWVAFGWYRADGTRRF
                     RYAYVELPRKNGKTTVAAGIALFLLIADGEAGAQVYVAATKKDQARIAFNAASEIARA
                     TPALRAAGRLKNEKAAVAVPSTASSLRPLSSDHSTMDGLNPSGAIIDELHEHKTPEVV
                     QKLRTATGARRQPLLWMITTAGIATECVGYNEHKYARNILDRVFEDDAYFALIASADA
                     EDDWASPATWAKANPNYGVSVKPPMLADMARRAVNDPSTLDELSRYHLNRWLLHIDSP
                     IPLHKWDECPATDAPNELVGRRCFLGLDLSNVADITAAALIFPPEGEAQTWRILMRYW
                     IPRDNMLERIQRDRIPYDRWAREGRITVTEGNVVDYDQVRADINALADQFEIVELGAD
                     PWNATQLTTQLLNDGLAVVAVRQGYATLSPATKFLQQLIYSRELDHGGCPVLRWMVGN
                     LAIQRDPSGNIKPAKDKATQKIDGVSALVTGLARAMLKMGAEEGPSMIAL"
     gene            complement(4957..5466)
                     /locus_tag="PLX85_00270"
     CDS             complement(4957..5466)
                     /locus_tag="PLX85_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HRC61641.1"
                     /translation="MAGLKGRSGGHNRKPTHLKLIEGTLGRNPQRANKREPKATTDVK
                     RKLRAPAWSGKLTRKWFRYFQREVNTLGVLGDTDEAALQGLAMAAAELEVAWREIQQR
                     GYTYEVVDEKGNVTIRPRPEVAIATRARKEMKQWFALFGLSPADRARVQVGKEPEDPF
                     EAFMRRGKA"
     gene            complement(5943..6221)
                     /locus_tag="PLX85_00275"
     CDS             complement(5943..6221)
                     /locus_tag="PLX85_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61642.1"
                     /translation="MGQPFLIDPTAGLIYTFDFSDEVPAGVSVTAIAIVAPAPLTTSG
                     KTDDLPNKQTRVLISGAIHGQTYQVEATATLSNGETVPLSATLRGWNS"
     gene            complement(6231..7862)
                     /locus_tag="PLX85_00280"
     CDS             complement(6231..7862)
                     /locus_tag="PLX85_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61643.1"
                     /translation="MQVGARYDSAAANVYQFRYSPSTPIVQRVTGGSPTTILTLGSSP
                     AANDDVRIEVRDTSIEVYYNNVSIGSVTDATYTGANRAAIGGVGTSGRIDDFGCGSLP
                     TLSSPTATNVSSSGASLGATTDESAGTLYAVLDSAANLSGVTRTQVFQGKRADGATAA
                     FSQNGAISTATPSLSFTGLSAGVTYSYAIVQINGGYANLVTGSFTTEVPGASDGPGFV
                     IGDEAGFVGEGAVGAPNAATPGAMTGSINFAFGQSGVMSGAGALQGSIGLSLTPSGAL
                     SGVGALSGAVSLSFGQAGALLGVGALTSAITIALSASGALTGAGALVGNIPLALTAAG
                     GMSGAGALAGSVSFAWSVAGTLSTIGSGAMTGAIATAFAQTGALAGMGALTGSVALTF
                     AEVGALSGAGALLGSIPFAFTAAGALTGAGSGAMTGAISMNFAEAGALSGAGAFVGSI
                     PVAFSATGLLQGAGALVGAVPISFAQAGQLAGAGALFGLIGIAWDAAGLLSALGALGD
                     GSASRLILAGTQGRLIRANLRGRLVTAAIGSRIAH"
     gene            complement(8094..>9335)
                     /locus_tag="PLX85_00285"
     CDS             complement(8094..>9335)
                     /locus_tag="PLX85_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61644.1"
                     /translation="PPAGSRVASPTGSGSACTDAQPCSFDTALSVAAVIVLKDGTYPG
                     VGWNGSSEPRRILSGSTVQAQNPGGAVINGLWIGRSTRKDSNITVRDVRIEGGASLYN
                     TQRVTLKNVGVHGPLDVGTNDHTQGNTDNLIEDVWVWASGQRIIAINYRADRNVWRRV
                     IVRGDGCGTAACAGSGNPNVGVTIYDSADVSFQNVMVLDRVLASGDSPYADFACAQHT
                     PNQYLWGRNEWLGIVSLNAPDQALYCEPDQVITGAVSATIRNALLWNGYGLNLARSGR
                     FVLDGATITNKGQGDGVRVAPELAGSGSTVSRVTVGGSGRYAVNSAVAPSQCNVTGSW
                     QATYNQTQCSGAGTAPAVWTFPPRYGVDGSRFGEAGVNAAQAASLLPYPNEVRIKAEM
                     CAGTTRGLCSAVSVSEYLRTR"
BASE COUNT         1551 a         3180 c         2964 g         1640 t
ORIGIN      
        1 cgccgcgacc gggatcaggt cgccgagttg gcgtagtgcc gaacgctggt acccgacacg
       61 tcgacgagct gaccgcccgt gcgcatccag gcgaggaagc cgacctggcc gagcttcgtg
      121 tacgccgagt cggtgaaccg gaagaggctc atcgccatga cgtcacggat cttgtagttc
      181 ttgaacaggc cgaagaggat cgacttcgcg ttcgcagcca tcgccgcgac atcgttgttc
      241 acgatgatcg gacgacccag cagcgtgtcc ggcgcaccgc cggggctgcc gacctcatag
      301 cccggtacga agatcggacg cccctgggtg tccttgatct tgcggatcac cttcaccgag
      361 gagtccgcca tcatgtaccc ggcgccgcgg cgacgatagg ccggatcgac ggagtgctcc
      421 agatcgatca ggtcatcgta gatgacggtc gtcgtctggc ccgtggtgcc cgtcttgccg
      481 acggtggagc ccgtcacgac accgcgcggc tggccggagc ccgtgccggt cgtgtagtgc
      541 tggttcgtga tgcggcccag acgctcgccc agccgccccc ggatcagcgc ctcgacgtcg
      601 atgttcgagt cctgcaggag ctcgtacgga accgcgacga tcttcgagct gtacttgaag
      661 gtcgacagcg agacgacgcc gaaactcggg tcgagaccgg tcgccgtggt gttctcgccg
      721 atcagctcgc cgacttccgc cgtgccgtcg gtcgtcgggt agttgatctg ctcaccggac
      781 gacgtcgcga agacatcggc agcgtcgcgc atcccgccga actccttcag cgcctcgaag
      841 acgctggccg cgaccgtggt cggcacggtg tagccgcctt cggccgcggt cgtggtcgac
      901 atcgtgttgc gcaggctccg caggagcttc cactcctcgt tgctgacggc ctggtcgcca
      961 ccacgcgccc acttgcggaa gatctcccgc tcccgctcgt tccgggcctt cgcctgctcg
     1021 agcgacagac cgcccgtctc ggcaatcgtc tcgatcaccc gcgactcggc cgccatgaga
     1081 tccaggacct tctgttcccg gccgatctcg gcgtcgatgt cgtcgatccg tgcgatcttc
     1141 tcgtcgtaga ccttctgctg gtcgggaccc cacttcttcg aagtgtcctc gacgagcgcc
     1201 ttgaccacct tggcgagaga accgcgctcc tcccgcagag catttagctt ggacatgaac
     1261 aactccgttg gttgagagaa aacaaagccc gcgcaaggcg ggcttcagac gcttctgcgg
     1321 gagtccgctg aaacgaaggg tcagagagcg cgcgcgaagc gcgacgcctc cagatgcttg
     1381 gcttcccagt cgatcgcagg cgctgccgac tccagcagcg cctgcggcgt cttgtcgtac
     1441 accgagaggt cccaggcgtt cttcgcctct gcgaaggcct caaccttgcg atccgcaaag
     1501 ccgttgtcga cggcctcgtc agcgttgaac caggtctcgg catcgagcca ggcgcggatc
     1561 ttgtcctcga cgttgcccgt ctcggccacg tattcccgga tcaggtcctg gtcgaccttt
     1621 tcgagcagct cggccatcga cagcatgtcg gccgcgttgc ccgcggcgaa cgcccatgcg
     1681 cgatggatca tgaagtacgc gccatcggcg atctcaacct cggctgcggc cagagcgatc
     1741 caggaggccg ccgacaacgc catgccatca acgtgagcga tgatcttggc cgggtgctgc
     1801 cgaatcgccg tcgcgatcgc gcgcccgtcg aatacatcgc cacccggcga attgattcgc
     1861 aggtggatcg tcttggcctg gattccctgc agttccttga cgaaccctgc ggccgacacg
     1921 ccccagaagg gatcgatgac gtcgtagaga tagagtgttg cctcctcggc gtcactcttc
     1981 gcttgcgggg tcgggagcgc cggggtctgc cggttccgca ggatctgtag ccgggactgg
     2041 attcggttca tcggccttcc tcggtgcgcg gtagagcgca tctgcatttt cattcggtgg
     2101 caggttctcg agcttgcgga cctcgtcgat gctcatccag ccatcgccct gggcgccgcc
     2161 gagcgctgct cggtagaagt ccgagcgcga cttggcgtcg ccgcgcagca gaccattgac
     2221 gttgaactct gtgaagtagc gctcgcgcac tggccagatc ttccggttca gttcctgctc
     2281 gatgcggcgc aggtgcggat tgagcgtgaa cttcacgaac ccgagcacct gctgctcgag
     2341 gcccgatccc cagctcgtgc tcttctccgt ctcgccgatc atgaaaggcg gaacgccgaa
     2401 ggcacgtgcg atgtcgacca cctggaactt gcgcgactcc aggagctggg catcgaccgg
     2461 gttgatcgtc agcggcttcg ccgtcatccc gttgctcagg atgatcggca gggcggaatt
     2521 gggccctccc gtcgggctcg ctccatagcg ctcgatgaac attcggcgca tctgctcgag
     2581 ctgctcatcg tcatggcgca cgccggttgg ggactccagc accacgcggc tggtcagacc
     2641 cgcggccagc gtattggcac tgtgctgatc agccgccatc gcaattccga tggcgttcat
     2701 cgcggccgac tggatgaccg agagcgaccg ttcgccgttg aaacccagcg agctgaagtg
     2761 cagcatgtcc tcgaacggca cgccgtagga ctccgcacca tcggcgacga agtacaccgt
     2821 ctcgtaccgc caccgcgaag tgcgaatcgt cttgatgccg accgcgcgcg cgtcgactgg
     2881 cacgaacgcc tgcacgatgt tgttgctgtt gcggcggatg acggcaaagc catccccgta
     2941 caggaacatg caggcgagca catattccca gaacgatacg gtcgtcatcc gctcgttcgc
     3001 ctgttcgttg agcagccacc acaggggatg cttgaccgcg cggcgctcat cgccggcgcg
     3061 ctcgaatacc ggcagcggca gtgacccaat cactccagcc aggatgcgca cacaggcata
     3121 gaccgcagcg acattcatcg cattcgcggc cgtgacccgc acccccgcga cggtgccgtg
     3181 catcccgaag aaatcgtccc agttctccca ggtgatcgcc gggggctgga tgacgatgtc
     3241 atcgttgcgc ggtgaccagc ggttcagcgc gcctatgacc tgctcgagtg acatcagagc
     3301 gctatcatcg acgggccttc ctctgcgccc atcttgagca tggcgcgggc gagcccggtc
     3361 acgagagccg acacgccgtc gatcttctgc gtggccttgt ccttcgccgg cttgatgttg
     3421 cccgacggat cccgttggat cgcgaggtta ccgaccatcc atcgcagcac cggacaacct
     3481 ccgtgatcga gttctctcga gtagatgagc tgttgaagga acttcgttgc cggcgacagc
     3541 gtcgcgtagc cctgccgcac ggcgaccacc gccaggccgt cgttcaggag ctgcgtcgtc
     3601 agttgcgtcg cattccaggg atcggcccca agctcaacga tctcgaattg atcggcgagc
     3661 gcattgatgt ccgcgcggac ctggtcgtag tcgacgacat tcccctcggt gactgtaatc
     3721 cgcccctccc gcgcccagcg gtcgtagggg atccggtcgc gctggatccg ctcgagcata
     3781 ttgtcccgcg gaatccagta gcgcattaga atgcgccagg tctgcgcctc gccctccggc
     3841 gggaagatca gcgccgccgc cgtgatgtca gcgacattgg acaggtccaa cccgaggaag
     3901 cagcggcggc cgaccagttc gttcggcgcg tccgtcgccg gacactcatc ccacttgtgc
     3961 agcgggatgg gggaatcgat gtgcagcagc cagcggttca agtggtaccg cgacagctca
     4021 tcgagggtcg acgggtcatt gaccgcgcgc cgcgccatgt ccgcgagcat cggcggcttc
     4081 accgagacgc cgtaattcgg attcgccttg gcccacgtcg ccggtgacgc ccagtcatcc
     4141 tccgcgtccg ccgaggcgat cagcgcgaag taggcgtcgt cctcgaacac gcgatcgagg
     4201 atgttgcgcg cgtacttatg ctcgttgtat ccgacgcact cggtcgcaat cccggccgtt
     4261 gtaatcatcc agagcagcgg ctggcgtcgt gcaccggtcg cggttcgcag cttctgcacc
     4321 acctcgggcg tcttgtgttc gtgcagctcg tcgatgatgg cgccggaagg attcaatccg
     4381 tccatcgtcg agtggtcgct cgagagcggg cgcagcgagc tcgccgtcga gggcaccgcg
     4441 acggccgcct tctcgttctt caggcgcccg gcggcgcgca gcgcgggtgt cgcgcgcgcg
     4501 atctccgagg cggcgttgaa ggcgatgcgc gcctggtcct tcttggtcgc agcgacgtag
     4561 acctgcgcac cggcctcgcc gtccgcgatc agcagaaaga gcgcaatccc cgcagccacc
     4621 gtggtcttgc cgttcttgcg cggcagctcg acgtaggcgt agcggaagcg tcgggtgccg
     4681 tcggcccgat accagccgaa ggcgacccag acgatgaaca tctgccaggg ttcgagatgg
     4741 aagggctggt tcgcccactc cccttcgaac tgcacgagga agccgaagaa ctcaatcacg
     4801 tacgcggcgc gcttcggatc gaaccgcagg ccccgcttgt ggccgtgctt caggtcatcg
     4861 atgtggcgcg cgacggcctt gcgatgccac tctccggccg cgatcttccc ggacatgacg
     4921 ccatcgcagt acgcgcggac gacatccgcc gtgctcttag gctttgccac ggcgcatgaa
     4981 ggcctcgaac gggtcctccg gctccttgcc cacctgcacc cgcgcccgat ccgccggcga
     5041 gagtccgaac agcgcgaacc actgcttcat ctccttgcgg gcgcgcgtgg caatggcgac
     5101 ttcggggcgc gggcggatgg tgacgttgcc cttctcgtcg acgacctcgt aggtgtagcc
     5161 gcgctgctgg atctcccgcc acgccacctc gagctcggca gccgccatcg ccaagccctg
     5221 cagggccgcc tcgtcggtgt cccccaggac gcccagcgtg ttgacctctc gctggaagta
     5281 gcggaaccac ttgcgcgtga gcttccccga ccaggctggc gcgcgcagct tgcgcttcac
     5341 atcggtcgtc gccttcggct cacgcttgtt ggctcgctgc gggtttctcc cgagcgtgcc
     5401 ctcaatgagt ttcaggtggg tcggtttccg gttatggccc ccggaccggc ccttcaaccc
     5461 agccatggat cctccaaatg caactcattt gctgtgggcg ctcacgcgat tcggaggcga
     5521 cgttcgcgcg cgacaggccg gagagatttc gccccccccc tatcccctcg cagcgtggaa
     5581 aatctcgcgg gcactcttgc tccggtgatg atgatcacac atgggctgat ggttgcccgc
     5641 atcccaaaaa cgcccaccgt gcgctgtagg tactatgtga tcgaccagca ccgcgggctg
     5701 gattcgaccc tcctccttgc atgccacgca gagtggatgc tgggcaagat actgcttggc
     5761 gtacatgcgc cacgaacgac tacggtactg agcatccaga gcgcgacgtt gcgggtcggc
     5821 tcgtctcacc cgcgcctcct cgcgccacgt cttccgacga tgctcgatgc agtaccgatc
     5881 gcctcggttc accagggcgg agcagccagg gtgactgcat ggccgtggtg cggcaatggg
     5941 catcagctat tccagcccct gagcgtcgcc gacaagggaa cggtctcccc gttgctcagc
     6001 gtcgcggtgg cctcaacctg gtacgtctgt ccatgtattg cgccgctgat gaggacacgg
     6061 gtctgcttgt tcggcaggtc gtcggtcttc ccggacgttg tgagcggggc cggcgccaca
     6121 atcgcgatcg cggtgacaga cacccccgcg ggaacctcgt cggagaagtc aaaggtgtag
     6181 atcaatcctg ctgtcggatc gatcaggaaa ggctgcccca tgcgaagtcc tcagtgagca
     6241 atgcgcgagc cgatcgcggc ggtgacgagc ctcccccgca agttcgctcg aatcagtcgg
     6301 ccctgagtgc cagcgagaat caacctggac gccgatccgt cacccagcgc acccagagcc
     6361 gacaacagac ctgctgcgtc ccacgcaatc ccgattaggc cgaacagcgc gccagcccct
     6421 gcgagctgtc ccgcttgcgc gaacgatatg ggcacagcgc ccacgagggc gccggcgccc
     6481 tgcagcagac ccgtggcgct gaatgcaacc gggatcgagc cgacgaacgc accagcaccg
     6541 ctcagcgcgc ccgcttccgc aaagttcatg gagatggcgc ccgtcatggc gccggagccg
     6601 gcgcccgtga gcgcaccagc ggcggtgaag gcgaacggga tcgaacccaa cagcgctccc
     6661 gcgccgctga gcgcaccgac ctcggcgaac gtcagagcca cggatccagt cagtgcgccc
     6721 atgcccgcaa gcgcaccggt ctgcgcaaag gccgtggcaa tggcacccgt catggcgccg
     6781 ctgccgatcg tagagagcgt gccggcgacg gaccaggcga acgagaccga gccggcgagc
     6841 gctcccgcgc cactcatccc accggccgct gtcagcgcga gcgggatgtt gccgaccagc
     6901 gcgcccgcgc cggtcagtgc tccgctcgca ctcagagcga tcgtgatcgc actcgtcaac
     6961 gccccgacgc cgagcagggc gcccgcctgg ccgaagctca ggctgacggc gccgctcagt
     7021 gcgccgaccc cactcaaggc gccggacggc gtgaggctca acccgatcga gccctgcagc
     7081 gcgccagcac cgctcatgac tccggactgc ccgaatgcga agttgatcga gcccgtcatg
     7141 gcgccaggcg ttgccgcgtt cggtgcaccg acggcgccct cgccgacgaa accggcctca
     7201 tcgccgatga cgaagccggg gccatcgctc gcacccggga cttcggtggt gaagctcccg
     7261 gtgacgaggt tggcataccc gccgttgatc tggacgatcg cgtacgagta cgtgaccccc
     7321 gccgacaggc ccgtgaacga gaggctcggc gtcgctgtcg agatcgcacc attctgcgag
     7381 aacgcagcgg tcgcaccgtc cgctcgcttc ccttggaata cctgggtgcg tgtgacacca
     7441 ctgaggttcg ccgcactgtc gaggaccgca tagagagtgc cggcgctttc atccgtcgta
     7501 gcgcccagcg aagcaccgct cgagctgaca ttcgtcgcgg tcgggctcga gagcgttggc
     7561 agacttccgc agccgaagtc gtcaatgcga cccgaggtgc cgaccccgcc gatcgcggca
     7621 cgattcgccc cggtgtacgt agcatcggtg actgagccga tggatacgtt gttgtaatag
     7681 acctcgatcg aagtatcgcg cacctcaatg cgcacatcgt cattcgccgc aggcgaggat
     7741 cccagcgtca ggatggtggt cggcgagccc ccggtgaccc gctgaacgat cggcgtactc
     7801 ggcgaataac ggaattgata cacgttggcc gccgccgagt cgtagcgtgc accgacctgc
     7861 accgcgtcag tactcgtcgg aagcgtcggg atcttgatct gcgaccagca attggccgga
     7921 ggtgtcccac cgttccagta cgcacccgat cccggcgaag cggcgggctg gacctgattc
     7981 gaaatgatcg agtgagcggt gccctgctcc gtccagctcg ctccgagccc tccgtctgct
     8041 cggttgaagt tgtcagagaa tgagacgccc acggatggac cgagagcgcc cgctcagcgc
     8101 gtgcgcaggt actccgacac gctcaccgcc gagcacagcc cgcgcgtcgt gccggcgcac
     8161 atttcggcct tgatcctcac ctcgtttggg tatggcagca gcgatgcggc ctgggccgcg
     8221 ttgactccgg cttcgccgaa gcggctaccg tcgacgccgt agcgcggcgg gaaggtccag
     8281 accgccggcg cagtgccggc gccgctgcac tgggtctggt tgtaggtcgc ctgccacgag
     8341 cccgtgacat tgcactgcga gggcgcgacg gccgagttca cggcatagcg gccactgccg
     8401 ccgaccgtca cgcgcgagac cgtgctgccg ctgccggcga gctcgggcgc gacgcgcacg
     8461 ccgtcgccct ggcccttgtt ggtgatcgtc gcaccatcga gcacgaagcg accgctgcgc
     8521 gcgaggttga gaccgtagcc attccagagc agggcatttc ggatcgtcgc cgagaccgca
     8581 cccgtgatga cctggtccgg ctcgcagtag agcgcctggt ccggcgcatt gagcgagacg
     8641 atgcccagcc actcgttgcg accccacagg tactggttcg gcgtgtgctg cgcgcaggcg
     8701 aagtccgcgt agggcgagtc gcccgaggcg agcacccgat cgagcaccat cacgttctgg
     8761 aagctcacgt cggccgagtc gtagatcgtc acgccgacat tcggattgcc agagcccgcg
     8821 caggccgccg tgccgcagcc gtcgccgcgc acgatgacac gacgccagac gttgcgatca
     8881 gcccggtagt tgatcgcaat gatgcgctgg ccggaggccc agacccagac gtcctcgatg
     8941 aggttgtcgg tgttgccctg ggtgtgatcg ttcgttccca catcgagcgg accgtgtacg
     9001 ccgacgttct tgagcgtcac gcgctgcgta ttgtagagac tggcgccgcc ctcgatgcgc
     9061 acatcgcgaa ccgtgatgtt gctgtccttg cgcgtactgc ggccgatcca caggccattg
     9121 atgacggcgc cgcccggatt ctgcgcctgg acggtgctac cgctcaggat gcgccgtggc
     9181 tcgctcgatc cattccagcc gacaccggga tacgtgccgt ccttgagcac gatgacggct
     9241 gcgaccgaga gggcggtatc gaagctgcag ggctgcgcgt cggtgcacgc cgagccgctg
     9301 cccgtgggcg atgccacgcg ggaacccgcc ggcgg
//
LOCUS       DAORPG010000005         8576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_317_length_8576_cov_3.491022, whole genome shotgun sequence.
ACCESSION   DAORPG010000005 DAORPG010000000
VERSION     DAORPG010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8576
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_317_length_8576_cov_3.491022"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PLX85_00290"
     CDS             complement(<1..348)
                     /locus_tag="PLX85_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRC61645.1"
                     /translation="MASHSQHEWLETTYAKATERAKERQPRFESSSGAEVAPLYGPED
                     LAEWNYFEKLGYPSEYPFTRGVQPTMYRGRLWTMRQYAGFGDAEESNRRYQFLLSQGQ
                     TGLSVAFDLPTQIG"
     gene            complement(466..542)
                     /locus_tag="PLX85_00295"
     tRNA            complement(466..542)
                     /locus_tag="PLX85_00295"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(506..508),aa:Val,seq:tac)
     gene            complement(669..1580)
                     /locus_tag="PLX85_00300"
     CDS             complement(669..1580)
                     /locus_tag="PLX85_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61646.1"
                     /translation="MSSKRILAFLVPAIVVAALAVALSGGTPSQARESSSPSTQPVQS
                     QATNPTKVFECFRVDASSDQPGADAKATVRLVTENFGGKLVRVRKLVAMCELAYKFPP
                     PPAGVPDEVFPPTDADVRVFACYQVQRGQDPNDPFVLATQNFGKDTVQVRASQQMCEE
                     AAKIRTDAAGNTTTVGQPSGVVWQCFNLDKAKSINQKFRVVTNNFGRDDAFVVKGAQL
                     CEEARKDRITASGEVTHSGEATGRVVECFRLESKLDPKAKVTLITKNFGAVDVLVRRA
                     TQMCEPGEKTPVYTLPNDPALDQPGDD"
     gene            complement(1800..3131)
                     /gene="mgtE"
                     /locus_tag="PLX85_00305"
     CDS             complement(1800..3131)
                     /gene="mgtE"
                     /locus_tag="PLX85_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRC61647.1"
                     /translation="MTSDALDIRPILAVPQNSSDGWQTLSEEDRFDFFQSLPRGEASE
                     LFLSLSAADQEALVSALPVSERRVWLRLLAPDDAADLIQLAREEERDGLLGLLVPRTR
                     IEVEALLEYQADVAGGRMNPSYAWVRPEMTAAQAQAYVRLQAADRISMVYYSYVLDRD
                     ERLLGVVSFRELMTAPAHRPVSEIMLRDPVCVAEDLDQEAVAQILAEHDLLAVPVVDA
                     GGHMKGIITVDDVIDVVQQEATEDIQKIGGSEALTLPYFRTRPAEMLRKRAGWLSLLF
                     LSEMLTATAMGFFEHEIEAAVVLAVFVPLVISSGGNSGSQASTLVIRAMALGEVRLRD
                     WWRVVRRELGFGLSLGAILATIGAIRITVWQGIFGSYGDHAFQLAMTVSLSLVGVVAW
                     GTMVGSMLPLALRRAGFDPANASAPFVATLCDVTGLVIYFSVAKLILLNSL"
     gene            3350..4216
                     /locus_tag="PLX85_00310"
     CDS             3350..4216
                     /locus_tag="PLX85_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRC61648.1"
                     /translation="MNDPFNDLMEPPHEPDPEEDQLLPHVPEHRFFAGPQSRWSELWW
                     ALRVFREFIAGFRTLHFVGPCVTVFGSARYSAGHPYYELAREMGRQLSRAGFSVMTGG
                     GPGIMEAANRGAREGGGPSIGCNIRLPREQAPNPYLDTWIEFDHFFARKVMLVKYSYA
                     FVVMPGGFGTLDEAAEAMTLIQTKKIKDFPVIFMGTQFWEPFRQFVRQSLLGQLTVDP
                     DDVDRVLFTDSPEIAVEAIRRAAIEKFGLEYGPLRPRRRWWLGEFGLARPRRAEAAGS
                     REVRSGPGRRRS"
     gene            complement(4185..4568)
                     /locus_tag="PLX85_00315"
     CDS             complement(4185..4568)
                     /locus_tag="PLX85_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRC61649.1"
                     /translation="MSADELLTHVTPSQRVAGDPTPGMTREAAHVSDGLWSGIAFTEP
                     GMTSGWHHHGEYETVIYVASGGLRMEAGPDGTQLIEAGPGDFVYVPPGAVHREGNPSA
                     EQSELVVVRTGHGPAVTNVDGPARS"
     gene            complement(4555..4776)
                     /locus_tag="PLX85_00320"
     CDS             complement(4555..4776)
                     /locus_tag="PLX85_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61650.1"
                     /translation="MVLGRHLLVLAALVVNLWLGGDIVEAWGGHALRSAFEAALILGY
                     FAWVLRFGNPRGPHAEGTNNNEEARDVRR"
     gene            4759..5313
                     /locus_tag="PLX85_00325"
     CDS             4759..5313
                     /locus_tag="PLX85_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61651.1"
                     /translation="MSAQHHQRILQEPPTPDDAPPLADSFTAALDGLSLIEIEEAAGE
                     LHRRIAEALRDIGERRADRGGRESPRARVASLVVATELASTLLSSAAAVSEIVLPRLE
                     VHPAAIAAVMYAAPTLPALLGRLEQDRRLLASLARAIESRLDEEANSPWGRMPLRRVV
                     TEMSIVRPAKVAMDLDVLAGQGEV"
     gene            complement(5298..6104)
                     /locus_tag="PLX85_00330"
     CDS             complement(5298..6104)
                     /locus_tag="PLX85_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HRC61652.1"
                     /translation="MGQRIVQVDAFTSTPYRGNPAAVCILDGAASERWMQDIAREMNL
                     SETAFLYPIEEGYALRWFTPAVEVDLCGHATLASAHVLWEEGHLMPGTDAVFETNSGR
                     LTARLSGDWIELDFPATPAVEVASPPGLEAALGVETLWTGRSEFDFLVEVDSEETVRE
                     LRPDLTLLGELHCRGVMVTSRALSEEFDFVSRFFAPAVGIDEDPVTGSAHCALGPYWA
                     QQLGKTELNAAQVSARGGVLRVRVEGERVILGGQAVTVLRGELSETYTSP"
     gene            complement(6107..8299)
                     /locus_tag="PLX85_00335"
     CDS             complement(6107..8299)
                     /locus_tag="PLX85_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HRC61653.1"
                     /translation="MAKPRVSAAGGFPAIVYTARKAFQSGNPIAFVGRMRSRNACKTC
                     ALGMGGLRGGMVNEAGHFPEFCKKSVQAQVADMQGTISEDFFARTPLSQMRFMTPAEL
                     ERLGRLAFPVIAEPGDTHYRRIGWDEALDRAADALREAERDQVFFYSSGRSSNEAAFL
                     LQLVARAYGSSNVNNCSYYCHQASGVALNEVYGSGTASVTLEDLEQADFALVAGANPA
                     SNHPRLITQLVALRRRGGRVIVVNPLKELGLTRFRIPSQWRSLAFGSKVSDLYLQPHV
                     GSDVALFKALLKGVIEARAEDRPFLEAHTQGWEAVRADIEATPWDELIAACGVPRAQI
                     DEAVRLLVEARRGILMWAMGLTHHAHGVDNVQSLVNLALSLGWFGHPGCGLLPIRGHS
                     NVQGVGSMGVTPALRDSFAEALEHAYDIDVPRQSGLDTFACMEAAERGAMQAAVLLGG
                     NLFASNPDRDWAGRALRNVDLTVALTTKLNEGHVHGRGRTSLILPVLARDEEPQPTTQ
                     ESMFSYVRLSAGGRPAVRGELRSEVDVIASLAERILPPGRFEWSRLRSHAALRETIAR
                     TVPGYDQLGLIEGAGGEFHVRGRRFHERASAMPDAKAHFRVTPLPDFTAGPDEFRLMT
                     LRSEGQFNSVVYDEEDLYRGNTRRDVVMMAQADADRLRVGEGDPVLVTSEAGKMRAVV
                     AIVDIAAGNLAMYYPEANALVPRRIDPRSKTPAFKSVVARVTPLPPRG"
     gene            8354..>8576
                     /locus_tag="PLX85_00340"
     CDS             8354..>8576
                     /locus_tag="PLX85_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRC61654.1"
                     /translation="MPRTAAIVLAGGRSRRMGRDKAALVLRGRSLLQRALDAVGAVVD
                     EIVVVGAPGRALPEVRSARKDLMPYSIDVV"
BASE COUNT         1311 a         2946 c         2956 g         1363 t
ORIGIN      
        1 cccgatctgg gtcggcaggt cgaacgcgac ggagaggccg gtctggccct gcgagagcag
       61 gaactggtag cggcggttgg actcctcggc gtcgccgaac ccggcgtact ggcgcatcgt
      121 ccagagccgc ccgcggtaca tggtcggctg cacgccgcgc gtgaacgggt actccgaggg
      181 gtatccgagc ttctcgaagt aattccactc cgcgaggtct tcgggaccgt agagcggcgc
      241 tacttcagcg ccggacgagg attcgaagcg aggctggcgc tccttggcgc gctcggttgc
      301 cttcgcgtag gtcgtctcga gccattcgtg ctgactgtgg cttgccacgt agcccccctg
      361 ctcgcgcaat acccgctggg gcgcgtgctc ccgagatggg gcagcgcctg cgaatcatac
      421 caatcggccg tacttgggag cagcaacgcg gcatagcgcg gcgtgtggtg ggcgatagag
      481 ggctcgaacc tctgaccttc cgggtgtaaa ccggacgctc tgaccagctg agctaatcgc
      541 cccgaggact gcgcgcgggg ctgcgttcga tgctagggcg ggcgcgagac ggcgacaact
      601 cggcgcccgt ggcgtgggag gccgggccgc gggccgcgac ctcccacgcg atcaccgacg
      661 agcggcgatc agtcgtcgcc cggctggtcg agcgccgggt cgttgggcag cgtgtagacc
      721 ggggtcttct cgccgggctc gcacatctgc gtcgcgcgcc gcacgaggac gtcgaccgcc
      781 ccgaagttct tggtgatcag cgtgaccttt gccttcgggt cgagcttcga ctcaagacgg
      841 aagcactcga ccacccggcc ggtcgcctca cccgagtggg tgacctcgcc gctggcggtg
      901 atgcggtcct tgcgcgcttc ctcgcagagc tgcgcgcctt tgacgacgaa cgcgtcatcg
      961 cgcccgaagt tgttcgtcac gactcggaac ttctggttga tgctcttggc cttgtcgagg
     1021 ttgaagcact gccagacgac gccgctgggc tgccccaccg tcgtggtgtt gcctgccgcg
     1081 tccgtccgga tcttggccgc ttcttcgcac atctgctgcg aggcgcgcac ctgcacggtg
     1141 tccttcccaa agttctgcgt ggcaaggacg aacgggtcgt tggggtcctg gccacgctgg
     1201 acctggtagc aggcgaagac gcgcacgtcg gcgtctgtgg gcgggaacac ctcgtcaggc
     1261 acgcccgcgg gcggcggcgg gaacttgtag gccagctcgc acatcgcgac gagcttgcgt
     1321 acgcgcacga gcttcccgcc gaagttctct gtcacgaggc gcacggtcgc cttcgcgtct
     1381 gcgccgggct ggtcactgga ggcgtcgacg cggaagcatt cgaacacctt cgtggggttg
     1441 gtggcctgcg actggactgg ctgtgtcgag ggcgagctcg attcgcgggc ctgactggga
     1501 gtcccgccgc tgagggccac cgccagtgcg gcgaccacga tggccgggac gaggaacgcg
     1561 aggatcctct tcgatgacat ggtcatgctc cttcggtccg ccccgggtcc gcaaccgcgg
     1621 gccgggggag gcgtcgaacg tctggatgca gggtgaggcg gtgtcagtct ctctccatcg
     1681 cgcctcccga ggtccggtgc ggaggacgcg cggggtggcg cggccgtagc ggccgtgcct
     1741 cgaagcgcgg cggcagtcta gcacggacga tcgagcggcg ggcggcgccg ccgaggcggc
     1801 tagagagagt tgagcaggat cagcttcgcc acggagaagt agatcaccag gccggtgacg
     1861 tcgcagagcg tcgccacaaa cggtgccgag gcgttggccg ggtcgaaccc ggcccgccgc
     1921 agggccagcg gcagcatcga ccccaccatc gtgccccagg ccacgacccc gaccaggctg
     1981 agcgacaccg tcatcgccag ctggaaggca tggtcgccgt agctcccgaa gatcccctgc
     2041 cacacggtga tgcggatggc gccgatggtg gccaggattg ccccgagcga gagcccaaag
     2101 ccgagctcac ggcgcacgac gcgccaccag tcacgcaacc gcacttcgcc gagcgccatc
     2161 gcgcggatca ccagcgtgga ggcctgcgag ccggagttgc cgccgctcga gatgaccagc
     2221 gggacgaaga cggcgagcac cactgccgcc tcgatctcgt gctcgaagaa ccccatcgcc
     2281 gtggcggtaa gcatctcgct gagaaacagc agcgagagcc agcccgcgcg cttgcgcagc
     2341 atctcggcgg gccgcgtacg gaagtacggc agcgtcagcg cctcgctacc gccgatcttc
     2401 tggatgtcct cggtcgcctc ctgctgcacg acatcgatca cgtcgtcgac ggtgatgatg
     2461 cccttcatgt ggccgccggc gtcgacgacc ggcaccgcca gcaggtcgtg ctccgcgaga
     2521 atctgcgcca ccgcctcctg gtcgaggtcc tcggcaacgc ataccggatc acgcaacata
     2581 atctcactga cggggcggtg tgccggcgcc gtcatcagct cgcggaacga cacgacgccg
     2641 aggaggcgct cgtcgcggtc gaggacgtag gagtaataga ccatcgagat gcggtcggcc
     2701 gcctggagac ggacgtatgc ctgcgcctga gcggccgtca tttccggccg cacccacgcg
     2761 tacgaggggt tcatgcggcc gcccgcgacg tccgcctggt actcgaggag ggcctccacc
     2821 tcgatgcgcg tgcgcgggac gagcagcccg aggagcccgt cccgttcctc ctcgcgagcg
     2881 agctggatca ggtccgcggc gtcgtcaggc gccagcagcc gcagccagac gcgccgctcg
     2941 ctgacgggga gtgcgctgac cagcgcctcc tggtccgccg cggatagcga caggaacagc
     3001 tcggatgcct cgccgcgggg cagcgactgg aagaagtcga atcggtcttc ctcggacagc
     3061 gtctgccatc cgtcggacga gttctgtggg actgcgagga tcggccggat gtcgagtgca
     3121 tcggacgtca tacgcgcaca cctccgcccg gccggggcgg aaggggcgcc cggtcgagct
     3181 agctcatgaa gccgctggta ctactgggat cgcccacgca gggggctcct ctctccggga
     3241 cgttctacca acgattctgc tcctgcctgc aagctctgtc caaccagatg ccggacattg
     3301 cgaggctttc gctcttgccg tcgcgcacgg agcccggaac gatggagacg tgaatgatcc
     3361 gttcaacgac ctgatggaac cgccgcacga gcctgatccg gaggaggacc agcttctccc
     3421 gcacgtcccg gagcatcgct tcttcgctgg tcctcagagc cgctggtccg agctgtggtg
     3481 ggcgctacga gtgtttcgcg aattcatcgc gggtttccga acgcttcact ttgtcgggcc
     3541 ctgcgttacg gtcttcggat ccgctcgcta ctcggccgga catccctact acgaactcgc
     3601 tcgggagatg ggccgccagc tttcgcgagc gggattctcg gtcatgacgg gcggcggccc
     3661 gggcatcatg gaagccgcga atcgaggcgc gcgggagggc ggcgggccca gcatcggctg
     3721 caacatccgc ctgccccgcg agcaggcgcc caacccgtac ctcgatacgt ggatcgagtt
     3781 tgaccacttc ttcgcgcgca aggtgatgct cgtgaagtac tcgtacgcgt tcgtcgtgat
     3841 gccgggcggc ttcggcaccc tcgacgaggc cgccgaagcc atgacgctga tccagacgaa
     3901 gaagatcaag gacttccccg tcatcttcat ggggacgcag ttctgggaac cgttccggca
     3961 gttcgtgcgg cagagcctgc tcggtcagct gacggtcgac cctgacgacg tggaccgcgt
     4021 cctgtttacg gactcgcccg agatcgccgt cgaggcgatc cggcgcgcgg ccattgagaa
     4081 gttcggcctc gagtacggcc cgctgcgtcc tcggcggcgc tggtggctgg gggagttcgg
     4141 tctcgcccgg ccgcgccgtg ccgaggcggc cggctcgcga gaggtcagga gcgggccggg
     4201 ccgtcgacgt tcgtgactgc cggaccgtgg ccagtacgga cgacaaccag ctcgctctgc
     4261 tccgccgatg ggtttccctc tcggtgcacg gcgcccggcg gcacgtacac gaagtcgccc
     4321 ggccctgcct cgatgagttg cgtcccgtcc gggccagctt ccatgcgcag accgcccgat
     4381 gcgacgtaga tcacggtctc gtactccccg tggtggtgcc atcccgaggt catgcccggt
     4441 tcggtgaagg cgattcccga ccacagcccg tcggagacat gcgccgcctc gcgagtcatg
     4501 ccgggcgtcg ggtctccagc cacccgctgc gagggggtga cgtgcgtcag tagttcatcg
     4561 gcggacatcg cgagcctcct cgttgttgtt cgtcccctcg gcgtgcggcc cgcgcggatt
     4621 gccgaagcgg agcacccacg cgaagtatcc gaggatgagc gcggcctcga atgccgaccg
     4681 aagggcatgt ccaccccacg cctcgacgat gtcgccgccg agccagaggt tgaccaccag
     4741 cgccgccagg acgagaagat gtctgcccag caccatcagc gcatcctaca ggagccaccg
     4801 acgccggacg acgccccgcc actcgccgac agcttcactg ccgccctgga tgggctgagc
     4861 ctcatcgaga tcgaggaggc ggccggtgag ctgcaccgcc gtatcgcgga ggcgctgcgg
     4921 gacatcggcg agcggcgcgc cgaccgcggc ggccgggaga gtcctcgtgc ccgtgtcgcg
     4981 tccctggtcg tcgccactga gcttgcctcc acgctgctgt cgtcggccgc cgcggtttcg
     5041 gaaatcgtgc ttcctcgcct cgaggtgcat ccggcggcca tcgccgcggt catgtatgcc
     5101 gcgccgacgt tgcccgcgct tctcgggcgc ctcgagcagg atcgccggct gctcgcgtct
     5161 ctcgcgcgag caatcgagtc acggctggac gaagaagcca actcaccctg gggccgcatg
     5221 ccactgcgcc gtgtcgtgac cgagatgtcg atcgtgcggc cggcgaaagt cgcgatggac
     5281 ctcgacgtgc tggccggtca aggcgaggtg taggtctcgc tgagttcgcc acgcaggacg
     5341 gtaactgcct ggccgccgag gatgacgcgc tcgccctcga cgcgcacgcg cagcacaccg
     5401 ccccgcgccg agacctgcgc cgcattcagc tcggtcttcc cgagctgctg cgcccagtag
     5461 gggccgaggg cgcagtgggc cgacccggtc accgggtcct cgtcgatgcc gacggccggt
     5521 gcgaagaacc gcgacacgaa gtcaaactcc tcggacaggg cacggctcgt gaccatcacg
     5581 ccgcggcaat gcagctcgcc cagcagtgtc aggtccggcc gcagctcccg cacggtctcc
     5641 tcggaatcta cttccacgag gaagtcgaac tccgagcgtc cggtccagag cgtctcgacg
     5701 ccgagcgcgg cctccaggcc ggggggcgag gccacctcga cggccggggt ggccgggaag
     5761 tccagctcga tccagtcgcc ggacagccgc gccgtgagcc gcccgctgtt ggtctcaaag
     5821 accgcatccg tgccgggcat gaggtgtccc tcctcccaca gcacgtgtgc gctcgcgagg
     5881 gtggcgtggc cgcagaggtc gacctcgacg gccggggtga accagcgcag cgcgtagcct
     5941 tcctcgatcg ggtagaggaa cgcggtctcg gataggttca tctcgcgggc gatgtcctgc
     6001 atccagcgtt ccgaggccgc cccgtcgagg atgcagacgg ccgcggggtt gcctcgatac
     6061 ggcgtgctgg tgaaggcgtc gacctggacg atgcgctggc ccatcgctat cccctcggcg
     6121 gtagcggcgt cacgcgtgct accacggact tgaacgcggg cgtcttcgag cgtgggtcga
     6181 tccgtcgggg cacgagcgcg ttcgcctccg ggtagtacat cgcgaggttg ccggcggcga
     6241 tgtcgacgat cgccacgacc gcgcgcatct tgccggcctc gctcgtcacg aggacgggat
     6301 cgccctcgcc cacccgtagg cggtcggcgt ccgcctgggc catcatcacg acgtcccggc
     6361 gggtgttgcc gcggtagagg tcttcctcgt cgtagacgac gctgttgaac tgcccttcgg
     6421 agcgcagcgt catgaggcga aactcgtcag gccctgccgt gaagtcaggc agtggcgtga
     6481 cgcggaagtg ggctttcgcg tccggcatcg ccgaggcgcg ttcgtggaac cggcggcctc
     6541 gcacgtggaa ctcgccaccc gcgccctcga tgagaccgag ctggtcgtag cccggcacag
     6601 tccgtgcgat cgtctcccgc agggccgcgt gcgaccgcag ccgggaccac tcgaagcggc
     6661 ccggcggcag gatgcgctcg gccagtgacg cgatcacgtc gacctccgag cgcagctcac
     6721 cgcggacggc cggacgtcct ccggccgaga ggcggacgta gctgaacatc gactcctgag
     6781 tggtgggctg cggctcctcg tcgcgcgcga gcactggcaa gatcagcgag gtgcgcccgc
     6841 ggccgtgcac gtgcccctca ttgagtttgg tcgtgagcgc tacggtcaga tcgacgttgc
     6901 gcagcgcccg cccggcccag tcccggtccg ggttgctcgc gaacaggttg ccgccgagca
     6961 gcaccgccgc ctgcatcgcg ccccgttccg cagcctccat gcaggcgaac gtgtccagtc
     7021 cgctctggcg ggggacgtcg atgtcgtacg cgtgctcgag cgcttccgcg aaggagtcgc
     7081 gcagggcggg cgtgaccccc atcgagccga cgccctgtac gttcgagtgc cctcggatgg
     7141 gcaggaggcc gcaaccgggg tgcccgaacc atccgagcga cagggcgagg ttgacgagcg
     7201 actgcacgtt gtcgacgcca tgcgcgtgat gggtcagccc catcgcccac atgaggatgc
     7261 cgcgccgggc ctcgacgagc aggcggactg cctcgtcgat ctgcgcccgc ggcacgccgc
     7321 acgccgcgat caactcgtcc cacggcgtcg cctcgatgtc ggcgcgcacg gcctcccatc
     7381 cctgcgtgtg ggcctcgagg aacgggcggt cctcggcccg ggcctcgatc acccccttga
     7441 gcagcgcctt gaatagcgcg acgtcagagc cgacgtgcgg ctgcaggtag agatccgaga
     7501 ccttcgatcc gaacgcgagg ctgcgccact gagaaggaat ccggaatctc gtgagtccga
     7561 gttctttgag ggggttcacg acgatgacgc ggccaccccg tcgccgcagc gcgaccagct
     7621 gggtgatgag ccgcggatga ttgctcgccg gattggcccc cgcgacgagc gcaaagtcgg
     7681 cctgctcgag gtcctcgagc gtcaccgaag cggtgcccga cccgtacacc tcgttcaacg
     7741 cgacgccgga cgcctggtgg cagtaatagg agcagttgtt gacgttggag ctgccgtacg
     7801 cgcgcgccac gagctgcagc aggaacgccg cctcattgct cgatcggccc gagctgtaga
     7861 agaagacctg gtcgcgctcc gcttcgcgca gcgcgtccgc cgctcggtcg agcgcctcgt
     7921 cccagccgat tcgacggtag tgcgtgtcgc ccggctccgc gatcacgggg aacgcgaggc
     7981 gcccaagccg ttccagctca gccggggtca tgaaccgcat ctgggagagc ggcgtgcgcg
     8041 cgaagaaatc ctccgagatc gtcccctgca tgtcggcgac ctgggcctgc acggacttct
     8101 tgcagaactc gggaaagtgt cccgcctcgt tcaccatgcc gccgcgcagg ccgcccatcc
     8161 cgagggcgca ggtcttgcag gcgttgcgcg aacgcatgcg tccaacgaac gcgatggggt
     8221 tgccggactg gaacgctttg cgagccgtgt acacaatcgc ggggaagccg cccgccgcgc
     8281 tcactcgggg tttcgccatg tgagccagcc taggccgcgc ccgcagccgc gacaagcaca
     8341 cgaggtctcg ccgatgcccc ggactgccgc gatcgtgctc gccggtgggc gctcacgccg
     8401 gatgggccgc gacaaggccg cgcttgtcct cagaggccgc tcgctgcttc agcgcgcgct
     8461 cgatgccgtc ggggcggtcg tcgacgagat cgtcgtcgtt ggggcgcccg gccgtgcgct
     8521 gcccgaggtc cgctcggcgc gcaaggacct gatgccctac tccatcgacg tggtca
//
LOCUS       DAORPG010000006         8126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_360_length_8126_cov_3.571181, whole genome shotgun sequence.
ACCESSION   DAORPG010000006 DAORPG010000000
VERSION     DAORPG010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_360_length_8126_cov_3.571181"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="PLX85_00345"
     CDS             complement(<1..1129)
                     /locus_tag="PLX85_00345"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HRC61655.1"
                     /translation="MMQAYYAEGMTAPAVFDLSVRSLPSQRNVLIACGVEDVLAYLEA
                     LRFSPEAIGYLDGLGRFRPEFLERLASFRFTGTVRAIPEGTPVFAGEPLLEVVAPLPE
                     AQLVETFLLNQVTMQTVLASKAVRCVFAAAGRPVVDFGLRRTHGTDAGLKAAKAAYIA
                     GFAGTSNVLAGMQYGIPVMGTMAHSYIQAHASEEEAFRAFARQYPGTTLLVDTYDTIE
                     GVRALCRLTAEPGDEFRVSAIRLDSGDLETLAREARALLDAAGLQRVEIVASGGLEER
                     AVARLRASGAPIDSFAVGTQVGTSADQPVLDTVYKLAEYAGEGRIKLSTAKVTLPGRK
                     QVFRAFRDGVAERDVVGLADERLDGVPLLVEVMRDGRRVATA"
     gene            complement(1189..1776)
                     /locus_tag="PLX85_00350"
     CDS             complement(1189..1776)
                     /locus_tag="PLX85_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRC61656.1"
                     /translation="MASYDDRTALIVVDVQNDFAAPTGSLHVPGAEAAIPAINREIGR
                     AAAAGAVIVYTQDWHPARTPHFQRDGGVWPVHCVGGSEGAAFHPALDVREGPVIRKGT
                     EGEDGYSAFSVRNPDSGEQFATALEAELRERGIERIVIVGLATDYCVKETGLDAARLG
                     FPATVVMDAIRAVDLEPGDGERALDALREAGIALE"
     gene            complement(2073..3860)
                     /locus_tag="PLX85_00355"
     CDS             complement(2073..3860)
                     /locus_tag="PLX85_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC61657.1"
                     /translation="MDATRDEGREGLASWRLGRRAAIRGALLGATGVAAAALIGCGGD
                     DDGGGEPSGTASATGTSTAANDPRYPKDAGLPYAYNFPEPAGKTPKAGGTMHVAASWD
                     VATMDPTKSAAGGTITIPNMVYNRLLGIVGGPDVNPFKLDLEPELASSWERTPDGMTY
                     TFQIKPGTKWQNVPPLSGRPFVAADAKFAYDRYRTTGVHQSLWVNVASIDAVSDTTLK
                     INMKRPVADFLNPLGGRYQTLFPHELVDSGAIEKQAVGTGPMILKSATAGSAAVFEKN
                     PDYFEKKVLLDGFEFKLMPDQAARLAAFRAGQVEYAYSAASSIDDVNNLLGTNPQIQI
                     NMVAVSYGGIPFGMNLSHPKFQDERVRRAVQLAINTQQMTTIVWKGLAKTLPMLPWAF
                     IFDKEPTPESGLLGKWWRYAPAESKQLLQAAGAEGLTFNNQYYAYGDYLDRYSELLVP
                     MFKDVGITMTGGKVDYTEFNSQWVGAQLKEVTSSGWVTVGFDADNFFYNILFSKSPGN
                     RWRLNDPQIDTLAEKQQVELNVEARKAIHRQIWDRALDMAYYPPMPSQVAFEIYQPWL
                     RGIRFGGALGSNSSYYDWGDQVGEAWLDK"
     gene            complement(4031..5305)
                     /locus_tag="PLX85_00360"
     CDS             complement(4031..5305)
                     /locus_tag="PLX85_00360"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRC61658.1"
                     /translation="MPGALEGIRVLEFTLVVAGPMCGVLLSDFGADVIKVEPPGGEST
                     RQSRSVVANESKLFQAFNRGKRDIIVDLQQPEGREVIYRLMPSIDVVIVNYRYGVAPG
                     IGIDYETLKRYRPDLIYVESTGFGTRGALAQRGCSDVVAQAYSGIMAADGKVAEDGAP
                     VQSTFGAPADYATAMASAMAICAALYHRERTGEGQQINSSLLRTGVWLQTHNVNHEPI
                     TDALFDDVIRAQMDATRAAGGSYDDLIRIRTEQRFAGGLYYSGYAAKDGGIILGALTK
                     TNRDGFRAILGMEDEPSDQPGHDVLDPESQRLAVHFKEVIRAKMRERTVAEWIDIFDA
                     AGLPAAPVNFPEEIRDDPQVQAEGIFVPLEHDLTGPQSVVGPVVEMSRTPTVARLASP
                     PLGRDTRGVLLEAGYAEAEVDSLLASGAVAAG"
     gene            5476..6705
                     /locus_tag="PLX85_00365"
     CDS             5476..6705
                     /locus_tag="PLX85_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018854309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC61659.1"
                     /translation="MDWLDTEEQAAFRNQVRGFINERLPEFYRQQAVDNRPRGLEGDW
                     QENLVHGTPEAKQAATDWAKALNERGWVAPHWPQAYGGAGMTTMEQFIFNQEMAEADA
                     PMVGGAGLSLLGPTVLVHGTDEQKARLLPPTLSGEYLWAQGFSEPGAGSDLASLQTRA
                     VRDGDEYVVNGQKLWTSTAHKSNWLFGMFRTDPEAPKHRGISFLVMDMSTPGLTVRPI
                     ISMGWEHATNETFYEDVRVPVANRIGEENRGWYVGVTLLDFERSNISGAIHIRRQLDG
                     LIGVAKSDEGQRWLQHGGLKRERHAIAEHYIESEVMFNFSFRIVSMQARGVIPNYEAS
                     TSKMYHSELLQRVSRTGIKTYGLYGNLWDPDEPLAPQKATFTQSNVHSVVMTIAGGSS
                     EIQRNIISTRGLGLPRG"
     gene            6838..>8126
                     /locus_tag="PLX85_00370"
     CDS             6838..>8126
                     /locus_tag="PLX85_00370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRC61660.1"
                     /translation="MAERAQAAAPRGVTTPASSGRPLDPDFARLLGRERGLSEAEAAR
                     RTAAGLANTDPSREVRDRDVVRRNTVTFFNLTLLALIGALLIVGEVRDGLFVGIVVGA
                     NVVVATIEELVATRRLRSLQALTAPGATVVRDGVTREVAAVEVVQGDVLVLAPGRQVV
                     ADGHLLGGACELDEALLTGESSTIRRAEGEAVRSGSYCVAGAAVYAAEEVGARAYAHQ
                     LTAQARERVRHETPLMIQFRRLLRVLLTATGVLAALLFIQFNIQDRGFAESLKATTAT
                     VTTVVPVGMLLGITVVTAVGALRVSRAGAIVQDMHAVEALNYVDVIAFDKTGTITSNR
                     LEVRDVVWAEDSAGEAPWLAAFSAAAREESRTVGAIAAALASAGAEATVVGRVPFSSA
                     RRWSALELERGGVRRVFVLGAPEAVLAGLPPEAALVSR"
BASE COUNT         1170 a         3105 c         2530 g         1321 t
ORIGIN      
        1 ccgccgtggc cacccgccgc ccgtcgcgca tcacctcgac gagcaacggc acgccatcga
       61 ggcgctcgtc cgccagcccg accacatccc gctccgcaac gccgtcccgg aacgcgcgga
      121 acacttgctt gcgtcccggc agcgtgacct tcgccgtcga gagcttgatg cgcccctcac
      181 cggcgtactc ggcgagcttg tacaccgtgt ccagtacggg ctggtccgcc gacgtcccga
      241 cctgcgtccc gaccgcgaac gagtcgatgg gagcccctga cgcgcgcagc cgtgccaccg
      301 cccgctcctc gagcccaccg ctcgccacga tctcgacccg ctgcaggccc gccgcgtcga
      361 ggagtgcgcg cgcctccctc gccagcgtct cgaggtcgcc cgagtccagc cggatcgcgc
      421 tcacccggaa ctcgtcgccc ggctccgcgg tcagccggca cagcgcgcgc acgccctcga
      481 tcgtgtcgta cgtgtcgacg agcagcgttg tccccgggta ctgccgcgcg aaggcgcgga
      541 acgcctcctc ttccgaggcg tgcgcctgga tatagctgtg cgccatcgtt cccatgaccg
      601 ggatgccgta ctgcatcccg gcgagcacgt tcgaggtgcc cgcgaacccc gcgatgtatg
      661 ccgccttcgc ggccttgagc ccggcatccg tcccgtgcgt gcgccgcagg ccgaagtcca
      721 ccaccggccg cccggcggca gcgaacacgc accgcacggc cttcgaggcc agcaccgtct
      781 gcatcgtgac ctggttgagg aggaaggtct ccaccagctg cgcctcgggc agcggcgcga
      841 cgacctccag cagcggctcg cccgcgaaca cgggcgtccc ctccgggatc gcccgcaccg
      901 tccccgtgaa gcgaaagctc gcgagccgct cgaggaactc cggtcggaac cgcccgagtc
      961 cgtcgaggta cccgatcgcc tcgggcgaga accgcagcgc ttcgaggtac gcgagcacgt
     1021 cttcgacccc gcacgcgatc agcacattcc gctgcgatgg caggctgcgg acgctcaggt
     1081 cgaagaccgc gggcgcggtc atcccctcgg cgtagtacgc ctgcatcatc gtcagctcgt
     1141 acaggtccgt gaggagcgcg acctcaggtg gcagttcggc gacgccgctc actcgagggc
     1201 gattccggcc tcgcgcagcg cgtcgagcgc gcgctctccg tcgcccggct cgaggtctac
     1261 cgcccgaatc gcgtccatca ccaccgtcgc cggaaacccc agccgcgcgg catcgaggcc
     1321 ggtctctttc acgcagtagt ccgtcgccag ccccacgatc acgatgcgtt cgatgccgcg
     1381 ctcacgcagc tccgcctcga gggccgtcgc gaattgctcg ccgctgtccg ggttccgcac
     1441 ggagaacgcc gagtagccgt cttctccctc ggtccctttc cggatcaccg gcccctcgcg
     1501 cacatcgagg gccgggtgaa acgccgcccc ctcggagccg ccgacgcagt gcactggcca
     1561 cacgccccca tcccgctgga agtgcggcgt gcgcgccggg tgccagtcct gcgtgtacac
     1621 gatgaccgcg ccggcggccg cggctcgccc gatctcgcgg ttgatcgccg ggatggccgc
     1681 ctccgcgccc gggacgtgca gcgacccggt cggtgcagca aagtcgttct gcacatcgac
     1741 cacgatgagc gcggtgcggt cgtcgtacga ggccatggcc accgattcta tccgagcgcg
     1801 tcaggacggc ggaaagttcg tccctgcgcc atctgccggc ctcggggaat gtgcctcccc
     1861 tcacctcgag gcgggccgtc gccgaaacca ctcgttccgc acgtcagcgt cgcttggacg
     1921 ccctcggatg gcccctcccc gtcggaggga gagggtgacg gcccgcgaag cagaccgcgt
     1981 tgcggacaca cgcagtcacg cagtacgaag gggaccgctc ccgaggaacg gcccccttcg
     2041 atgcacgtcc ggggacgcgc gcccgaagtg gcctacttgt ccagccacgc ctccccaacc
     2101 tggtcgcccc agtcgtagta cgacgagttc gaccccagcg cgccgccgaa gcggatcccc
     2161 cgcagccacg gctggtagat ctcgaacgcc acctgcgagg gcatcggcgg gtagtacgcc
     2221 atgtccagcg cgcggtccca gatctggcgg tggatcgcct tccgcgcctc gacgttcagc
     2281 tcgacctgct gcttctccgc cagcgtgtcg atctgcgggt cgttcagccg ccaccggttc
     2341 cccggcgact tcgagaacag gatgttgtag aagaagttgt ccgcgtcgaa gccgaccgtc
     2401 acccagccgc tcgaggtgac ctccttcagc tgcgcgccga cccactgcga gttgaactcc
     2461 gtgtagtcca ccttgccgcc cgtcatcgtg atcccgacgt ccttgaacat cggcaccagc
     2521 aattccgagt accggtcgag gtagtcgccg tacgcgtagt actggttgtt gaaggtcagc
     2581 ccctcggcgc ccgccgcctg cagcagctgc ttcgactccg ccggcgcgta ccgccaccac
     2641 ttccccagca gccccgactc cggcgtcggc tccttgtcga agataaacgc ccacggcagc
     2701 atcggaagcg tcttcgcgag gcccttccac acgatggtcg tcatctgctg cgtgttgatg
     2761 gccagctgca ccgcgcgtcg gacgcgctcg tcctggaact tcgggtggga caggttcatc
     2821 ccgaacggga tcccgccgta cgacaccgcg accatgttga tctggatctg cgggttcgtc
     2881 cccagcaggt tgttcacgtc gtcgatgctg ctcgccgccg agtacgcgta ctccacctgc
     2941 cccgcccgga atgccgcgag gcgagccgcc tggtcgggca tgagcttgaa ctcgaacccg
     3001 tcgagcagca ccttcttctc gaagtagtcc gggttcttct cgaataccgc cgcgctgccc
     3061 gccgttgccg acttcaggat catcggcccg gtgcccacgg cctgcttctc gattgcgccg
     3121 ctgtccacca gctcgtgcgg gaacagcgtc tggtaccgcc ctccgagggg gttcaggaag
     3181 tccgccacgg gccgcttcat gttgatcttc aacgtggtgt cgctcaccgc gtcgatcgag
     3241 gcaacgttca cccacagcga ctggtgcacg cccgtcgtgc ggtagcggtc gtacgcgaac
     3301 ttcgcgtccg ccgccacgaa gggccggccg ctcagcggcg gcacgttctg ccacttcgtc
     3361 cccggcttga tctggaacgt gtaggtcatc ccgtccggcg tgcgttccca gctcgaggcc
     3421 agctccggct cgaggtcgag cttgaacggg ttcacgtccg ggccgcccac gatcccgagt
     3481 agtcggttgt agaccatgtt cgggatcgtg atcgtcccgc ccgcagccga cttcgtcggg
     3541 tccatcgtcg ccacgtccca cgaggcggcg acgtgcatcg tccccccggc cttcggtgtc
     3601 ttcccggctg gctccgggaa gttgtacgcg tacgggagac ccgcatcctt cgggtaccgc
     3661 gggtcgttcg ccgccgtgct cgtccccgtc gccgacgctg tgcccgaggg ctcaccaccc
     3721 ccgtcgtcat cgcctccaca cccgatcagc gccgcagccg ccacacccgt ggcacccagc
     3781 aacgcccccc ggatcgccgc ccgcctcccg aggcgccagc tcgccagccc ctcacgtccc
     3841 tcgtccctcg tggcatccat cgcaacctcc gtccatcggc agcgcacggc tgcgcaatac
     3901 ggacgtgatt gtactccgta tgtaatgaag agatatcaac ggtgtgtgcg gtggttcgga
     3961 tgcgctcggc gctcagtgcc caccggcctc ctcgacggag gccggtggcg tcttgcttgg
     4021 tgtgggcgcg ctacccggcc gccacggctc cgctcgccag cagcgagtcg acctccgcct
     4081 cggcgtagcc cgcttcgagg agcacgccgc gcgtgtcccg tcccagcggt ggcgaggcga
     4141 gccgcgctac ggtcggcgtc ctcgacatct cgaccaccgg gccgaccacc gactgcggcc
     4201 ccgtcaggtc atgctccagc ggcacgaaga tgccctcggc ctgcacctgc gggtcgtcgc
     4261 ggatctcctc ggggaagttc accggcgccg ccgggagccc cgccgcgtcg aagatgtcga
     4321 tccactcggc aaccgtgcgc tcgcgcatct tggcccggat cacttctttg aagtgcaccg
     4381 ccagccgctg gctctccggg tcgagcacgt catgccccgg ctggtcgctc ggctcgtcct
     4441 ccatcccgag gatcgcgcgg aacccgtccc ggttcgtctt cgtcagcgcg ccgaggatga
     4501 tcccgccgtc cttggccgcg tagccgctgt agtagaggcc gcccgcgaag cgctgctccg
     4561 tgcggatgcg gatcaggtcg tcgtagctcc cgcccgccgc ccgcgtggcg tccatctgcg
     4621 cccggatcac gtcgtcgaac agcgcgtccg tgatcggttc gtggttcaca ttgtgtgtct
     4681 gcagccacac gcccgtgcgc aacagcgagc tgttgatctg ctgcccttcc cccgtgcgct
     4741 cccggtggta cagcgccgcg cagatggcca tcgccgaggc catcgcggtc gcgtagtccg
     4801 ctggcgcccc gaacgttgac tgcaccggcg cgccgtcttc tgccaccttg ccgtccgccg
     4861 ccatgatccc cgagtacgcc tgcgccacca cgtccgagca cccgcgctgc gccagcgcgc
     4921 cacgcgtccc aaatcccgtg ctctcgacgt agatgaggtc gggccggtac cgcttgagcg
     4981 tctcgtagtc gatgccgatg ccgggcgcga cgccgtagcg gtagttcacg atcaccacgt
     5041 cgatcgaagg catcagccgg tagattacct cgcggccctc gggctgctgc aggtcgacga
     5101 tgatgtcccg cttgccgcgg ttgaacgcct ggaagagctt gctctcgttc gccaccacgc
     5161 tgcggctctg ccgcgtcgac tcacccccgg gtggctccac cttgatcaca tccgccccga
     5221 agtccgagag cagcaccccg cacatcggtc cggcgacgac gagtgtgaac tcgaggacgc
     5281 gaatgccttc gagcgctccg ggcatggtgg ttccctcctc gctggaactg ggtacgtcgg
     5341 gcgtgatgtg gccgccagtc tacgggctcc gctcccgctg agcacgggcg tggtctgcta
     5401 caatcccgcc cggtttgcga tcagcgtcac gcgccggcac gagctccgct cggccgtacg
     5461 agaaagaggc acggcatgga ctggctggat acagaagagc aggcggcgtt ccgtaaccag
     5521 gtgcgtggct tcatcaacga gcggctcccc gagttctacc gccagcaggc cgttgacaac
     5581 cgcccgcgcg gcctcgaggg agactggcag gagaacctcg tccacggcac gcccgaggcg
     5641 aagcaggccg cgaccgactg ggcgaaggcc ctcaacgagc gcggctgggt cgccccgcac
     5701 tggccgcagg cctacggcgg cgccggcatg accacgatgg agcagttcat cttcaaccag
     5761 gagatggccg aggccgacgc ccccatggtg ggcggcgcgg gcctctctct cctcggcccc
     5821 accgtgctcg tccacggcac agatgagcag aaggctcgcc tcctgccgcc cacgctctcc
     5881 ggcgaatacc tctgggcgca gggcttctcc gagccgggcg ccggctcaga cctcgcttcg
     5941 cttcagaccc gtgccgtccg cgacggcgac gagtacgtcg tcaacggcca gaagctctgg
     6001 acctccaccg cccacaagtc caactggctc ttcggcatgt tccgtactga ccccgaggcg
     6061 cccaagcacc gcggcatctc gttcctcgtc atggacatga gcacgccggg cctgaccgtg
     6121 cgccccatca tctcgatggg ctgggaacac gccaccaacg agaccttcta cgaggacgtc
     6181 cgcgtccccg tcgccaaccg cattggcgag gagaaccgcg gctggtacgt cggcgtcacc
     6241 ctcctcgact tcgagcgctc caacatcagc ggcgctatcc acatccgccg ccagctcgac
     6301 ggcctgatcg gcgtcgccaa gtccgacgag ggccagcgct ggctgcagca cggcggcctc
     6361 aagcgtgagc gtcacgccat cgccgagcac tacatcgagt ccgaggtgat gttcaacttc
     6421 tcgttccgca tcgtctcgat gcaggcccgg ggcgtcatcc cgaactacga ggcctccacc
     6481 tcgaagatgt accacagcga attgctgcag cgcgtctcgc gtaccggcat caagacctac
     6541 ggcctctacg gcaacctttg ggatccggac gagcccctcg ctccccagaa ggccaccttc
     6601 acgcagtcga acgtccactc ggtcgtcatg acgatcgccg gcggctcgag cgagattcaa
     6661 cgcaacatca tctccacccg cggcctcggc ctgccgcgcg gctaaccctt cctctccgag
     6721 gcttacgagg agcgctcccg cgagggggcg ctcctcgttt ccccacccgc ctcgcttcgc
     6781 tgcacgctcc gcgtacgatg ctcgtgaaca tgcgtagccc cgatcgagtc aacgaggatg
     6841 gctgagcgcg cccaggcggc cgccccgcgc ggcgtgacga cgccggcgtc gtctggccgt
     6901 ccccttgacc ccgacttcgc ccggctcctc ggccgggagc gagggctcag cgaggcggaa
     6961 gccgcccgcc gcactgcggc cggcctcgcc aacaccgacc cctcccgcga ggtgcgcgac
     7021 cgcgacgtcg tccgccgcaa tacggtcacc ttcttcaacc tcacgctgct tgcgctgatc
     7081 ggggcgctgc tcatcgtcgg cgaggtacgc gacggcctct tcgttggcat cgtcgtcggc
     7141 gcgaacgtcg tggtcgccac catcgaagag ctcgtggcga cccgccgcct gcgctccctg
     7201 caggcgctta cggcgcccgg cgcgaccgtt gtccgtgatg gggtcacgag ggaggtcgcc
     7261 gccgtcgagg tcgtacaggg ggacgtgctc gtgctggccc ccggtcgcca ggtcgtcgcc
     7321 gacggtcatc tcctcggggg cgcctgcgaa ctcgacgagg cgctcctcac cggcgagtcc
     7381 tctaccatcc gtcgcgccga gggcgaggcc gtccgctccg gcagctattg cgtcgccggc
     7441 gccgccgtct acgccgccga ggaggtgggg gcgcgcgcct acgcccatca gctcaccgct
     7501 caggcccgcg agcgcgtgcg ccacgagaca cctctcatga tccagtttcg ccgcctgctc
     7561 cgtgtcctcc tcacggccac gggtgtcctc gccgccttgc tcttcatcca gttcaacatc
     7621 caggaccgcg gcttcgccga gtccctcaag gcaaccacgg cgaccgtcac caccgtcgtc
     7681 cccgtgggca tgctgctcgg catcacggtt gtcaccgcgg tcggcgcgct gcgcgtctcc
     7741 cgcgccggcg cgatcgtcca ggacatgcac gccgtcgagg cgctcaacta cgtcgatgtc
     7801 atcgcgttcg acaagaccgg caccatcacc tccaaccgcc tcgaggtgcg cgacgtggtc
     7861 tgggcggagg actccgccgg ggaagcaccc tggctcgccg ccttcagcgc cgcggcccgc
     7921 gaggagagtc gtaccgtcgg cgccatcgcc gccgcgctcg cctccgccgg cgctgaggcc
     7981 acggtggtgg ggcgcgttcc cttcagttcc gcccgccgtt ggagcgcgct cgaactggaa
     8041 cgcggcggcg tccgtcgcgt cttcgtcctc ggcgcacccg aggcggtcct cgccggcctg
     8101 cctcccgagg cggccctcgt cagtcg
//
LOCUS       DAORPG010000007         7957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_377_length_7957_cov_3.777145, whole genome shotgun sequence.
ACCESSION   DAORPG010000007 DAORPG010000000
VERSION     DAORPG010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 7957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7957
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_377_length_7957_cov_3.777145"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLX85_00375"
     CDS             complement(<1..198)
                     /locus_tag="PLX85_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61661.1"
                     /translation="MQAPVREPQVVLADEILRGVARVFERLPWYVADGARMSSDAFAL
                     LIVSDDPEEAVGEVVEQARTDL"
     gene            361..804
                     /locus_tag="PLX85_00380"
     CDS             361..804
                     /locus_tag="PLX85_00380"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRC61662.1"
                     /translation="MRREPLRRWSMLAALAVAGVIVVLTLVVSPAEGDPMSRLIPAIF
                     TASRSAGADTCVGGIPCQDGHALAFLALALFASVHVAAAWSTRRQLTRLVGLFVLLVA
                     FAAIDEFAQGWTGRDPSFADWLADTAGILLGVLVGAQLTRFLVRG"
     gene            912..1883
                     /locus_tag="PLX85_00385"
     CDS             912..1883
                     /locus_tag="PLX85_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61663.1"
                     /translation="MAALLLLLSACGGSDSHEAATAAAPADPAVTAVPTPTPRWPAVA
                     RATITTEDLNVRSGPGSGFPVVGRLQPNDVVPVSGRLAGAEWLALPGIGWTVYAEDWM
                     RLDVPFKDLPQVSDPAKAFEFAGPVYSADARSGIPVVDEVLAAIVSRDRTRLLALASA
                     PAPSPTASPTRTAGGTPTPVATPVSTLVPDVTEAAAAGTPTATPRSTACSDSPLPASR
                     LREFLDAFYRTDVPAASRGGDGQGVLRLYGVVRAPVPATGAPDYVLVVAFEGGEGRQL
                     WVSPDGRLTQFTLPCEPTLPGSLLRVTSGEPFFWFRPAVKAPVRPLP"
     gene            complement(1887..2315)
                     /locus_tag="PLX85_00390"
     CDS             complement(1887..2315)
                     /locus_tag="PLX85_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRC61664.1"
                     /translation="MKLLIALETSEHDDSVAAPTVALARSSGAEVVLLNVINPLVDGA
                     DVVAASRAEALKTVHADRVAYLQHYATHFQPEARTRVEELEHGEDVPSCIARVAEDEQ
                     ADILAIATNRASGVRGLILGSVAQHLMRISPCPILVIRVE"
     gene            complement(2411..4273)
                     /locus_tag="PLX85_00395"
     CDS             complement(2411..4273)
                     /locus_tag="PLX85_00395"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC61665.1"
                     /translation="MAIEARPSAAIVRYPLPGEPSASSPHIPRLHSRSRVRERIGDAI
                     PLTLSLLLITFIFWAPFHSLTATVFAVSLVGFYVYWVGRSYSVAAACWIGLRRINRWK
                     RTEWPAKYAEWRERNSSAEAWDWPRHLVIIPNYRENEHELARTLDSLAAQANAGQLVV
                     ALAMEEREAGAQRKAARLLLAYRARFGDIFATFHPAGIPGETPGKGSNEAWAARQAHM
                     RLIEAGHGDLGRYTVTSCDADAFFDPHHFEALNYLFLTSKDRYRAFWQPAIFNSNNIW
                     DIPAPLRLPDGLSGVNRLANLVLPMSVKFPTSCYALSWQMLTEVDYWDEEVIPEDWHV
                     YLKCCFALGDRVHVEPLYLPLGNDCVLADGYRTTLRAHYTQSVRHAWGSSDVPYAWRA
                     TFAPGPLSRLRRWMLAANLTKVHVLWAAQWYIITLGMVVPTKLALHLGAPMPDWWLRK
                     QFTIPGPGWHLGNILTPQTWFAFDETGLIEPRITLSICGLLIAVCIVPLFALIAFEYR
                     TRGPRPAYVSRVQVAGQFLMWPLMAAITFVWASLPALHAQWKLASGRGLVYRVAQKGG
                     HNIQADEPFAELSEAERDAIVAAATAAANGEVKRPPEHDTVAAFQARAGNLLVF"
     regulatory      complement(4291..4442)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(4642..5115)
                     /locus_tag="PLX85_00400"
                     /pseudo
     CDS             complement(4642..5115)
                     /locus_tag="PLX85_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011124168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            5375..7198
                     /gene="typA"
                     /locus_tag="PLX85_00405"
     CDS             5375..7198
                     /gene="typA"
                     /locus_tag="PLX85_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRC61666.1"
                     /translation="MPFREDIRNVAIIAHVDHGKTTLVDAMLRQSGSFAAHQEVAERV
                     LDSMDLEREKGITILAKNTAVRYDGVKINIIDTPGHADFGGEVERGLTMVDAALLLVD
                     ASEGPLPQTRFVLRKALEANLAIILVVNKIDRPDARIKEVVDEVYELFLDLDATEAQI
                     DFPIVYCNARAGEASLDPAQPGADLKPLFDLLLNYVAPPSYEDDHPLQALVTNLDASL
                     YVGRLALCRVIQGTIRRGQLVARCRVDGTVERVRVTELYVTEALERVPAEEAGPGDII
                     AVAGFPDVTIGETLADADDPRPLPVIRIDEPSLSMTIGINSSPLAGQDGNKLTARLLK
                     NRLDQEQVGNVSIRILPADRPDTWTVQGRGELQLAVLVETMRREGFELTVGKPEVVTQ
                     VIDGRLSEPVERLAVDVPEDYLGVVTQLLALRKGRLEQMVNHGSGRVRMEYLVPARAL
                     IGFHTEFLTETRGTGLLHHVFDRYEPWHGELRTRPTGSMVADRRGPTTTYALLSLQER
                     GQMFVGPGIETYEGMIVGENARADDMDVNPTREKKLTNMRSSTSEETEKLVPHRQLSL
                     EQALEFIREDECLEVTPSHVRIRKVALDGIERARRRSARRA"
     gene            7395..>7957
                     /locus_tag="PLX85_00410"
     CDS             7395..>7957
                     /locus_tag="PLX85_00410"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HRC61667.1"
                     /translation="MAFFLAAIVVAVISFTFFLRDGALHSLGIGGGKSNDRSASALLV
                     KDPNIPTIKDLNQLRQQYGDPADATYGRILIPSIHLDAPIGARIVGADGQMPDPTGPS
                     DVVWYDFQHMPGFGGVPGTGHNAIFAGHVDRAAYLDYAGVNYIGAGIFYSLSELDRGD
                     TITIEVGGKSYRYIVAWVQDVKTESEWG"
BASE COUNT         1250 a         2884 c         2561 g         1262 t
ORIGIN      
        1 cagatcggtg cgcgcctgct cgacgacctc gccgaccgcc tcctccgggt cgtcggagac
       61 gatcagcagc gcgaacgcgt cggaggacat gcgcgccccg tccgcgacgt accaaggcag
      121 ccgctcgaag actcgagcga ccccgcgcag gatctcgtcg gcgagtacca cctgcggttc
      181 ccggacggga gcctgcaccc cgcgcttgtc gaatacgacg gatcggcgag ccagctctca
      241 cccgggctgg aggtcgcggt ggagggcctc ggcgcgccgc ccttctagcc atggagcgtt
      301 cagctactgt ggcctcgttg ccggccgggg taccatcgac ggccggacga gggggcagag
      361 gtgcgccggg aaccgctccg tcggtggtcg atgctcgccg cgctggccgt cgctggcgtc
      421 atcgtcgtgc tcacactcgt cgtcagtccc gccgagggcg accccatgag tcggctcatc
      481 cctgccatct tcaccgccag ccgatcggcc ggagcggaca cctgcgtcgg cggcatcccc
      541 tgtcaggacg gccacgccct ggcgttcctc gcgctcgccc tgttcgcctc ggtgcatgtc
      601 gctgctgcct ggtcgacccg ccggcaactc acgcgtctcg tcgggctgtt cgtcctcctc
      661 gtggcctttg cggccatcga tgagttcgca caggggtgga cggggcgtga tccctcgttc
      721 gccgactggc tggccgacac ggccgggatc ctcctcggcg tgctcgtcgg cgcacagctc
      781 acgcgcttcc tcgttcgagg ctgagcgcgg aacggaccgg ctgggcgcgt cgttagaatg
      841 agtacagagc gctgcgcgat ggaagtgggt gctcgttggc ctccctgcgg ccccttggac
      901 tcccgctttc ggtggctgcg ctgctgctcc tgctctccgc ctgcggcggc agcgatagcc
      961 acgaagccgc aacggccgcc gcgccggcgg atcctgcggt aaccgcggtt ccgacgccga
     1021 ccccccgctg gcccgcggtc gcccgtgcga cgatcaccac cgaggatctc aacgtgcggt
     1081 ccggcccggg ctcgggcttc cccgtcgtcg gccggttgca gcccaacgac gtggtcccgg
     1141 tttcgggacg tctcgcgggc gcggagtggc tcgccctccc cgggatcggt tggacggtgt
     1201 atgccgagga ctggatgcgc ctcgatgtac cgttcaagga tttgccccag gtgagcgacc
     1261 cggccaaagc gttcgagttc gctggacccg tctactccgc tgacgcgcgc tccggcatcc
     1321 cagtcgttga cgaggtgctg gccgcgatcg tcagccgcga ccgcacccgc ctcctggctc
     1381 tcgccagcgc acctgcccca tctccaaccg cgtcgccgac cagaaccgcc ggcggcacgc
     1441 cgaccccggt cgccacgccc gtgtcgaccc tggtcccgga cgtaaccgag gccgcggcag
     1501 cgggcactcc caccgcaacc cctcggagca ccgcgtgttc cgacagcccg ttgccggcaa
     1561 gcaggctgcg cgagttcctg gacgcgttct accggaccga tgtgcccgcc gcctcccgtg
     1621 ggggcgacgg ccagggcgtg ctgcggctgt acggggtggt gcgtgctccg gtgcctgcca
     1681 ccggagcacc cgactacgtc ctggtcgtgg ccttcgaggg cggcgagggt cgccagctct
     1741 gggtgagtcc ggacggtcgc ctcacgcagt tcacgctgcc gtgcgaaccc acgcttcccg
     1801 ggagcctgct ccgggtgacg agcggagagc cgttcttctg gttccgcccg gcggtcaaag
     1861 cgccggtgcg gccgctcccc tgatcgctac tccacccgga tcacgaggat cggacacggg
     1921 ctgatccgca tcaggtgctg tgccacgctg ccgagtatca ggccgcgcac acccgaggcg
     1981 cgattcgtgg caatggcgag gatgtccgcc tgttcgtcct ccgccacccg ggcgatgcaa
     2041 ctcgggacgt cctcgccgtg ctcgagttcc tccacgcgcg tccgcgcctc aggctggaag
     2101 tgcgtggcgt agtgctgcaa gtaggcgaca cggtcggcgt gcaccgtctt cagcgcctcg
     2161 gcccggctcg ccgccacgac gtcggccccg tccacaagcg gattgatgac gttcagcagc
     2221 acgacctcgg cgcctgagct gcgggcgagc gccaccgtcg gcgccgcgac gctgtcgtcg
     2281 tgttcggacg tctcgagggc gatcagcagc ttcatcttca ttcccgtctt cggccgcgag
     2341 cgcaggatac gcgcacgcgc gcttgtgcgc gtgcgcgtgc cttggctcgg acgctgcgag
     2401 tgaacgctga ctagaagacg agcagattgc ctgcccgggc ctggaacgcg gcaacagtgt
     2461 cgtgctccgg cggacgcttc acctcgccgt tggctgcagc cgtcgccgcc gccacgatgg
     2521 cgtcgcgctc ggcctccgac agctccgcga acggctcgtc ggcctgaatg ttgtgcccgc
     2581 ccttttgcgc gacgcggtag accaggccgc ggccgctcgc cagcttccac tgtgcgtgga
     2641 gggccggcag gctcgcccag acgaaggtga tcgccgccat gagcggccac atcaggaact
     2701 gcccggcgac ctgcacgcgc gagacgtacg cgggccgcgg gccgcgcgtt ctgtactcga
     2761 acgcgatcag tgcgaacagc ggcacgatgc agaccgcgat cagcagcccg cagatgctca
     2821 gcgtgatccg tggctcgatc aggccagtct cgtcgaatgc gaaccaggtc tgcggcgtca
     2881 ggatgttccc gaggtgccag ccgggacccg ggatggtgaa ctgcttgcgc agccaccagt
     2941 ccggcatcgg cgcgccgagg tgcagcgcca gtttggtcgg caccaccatg cccagtgtga
     3001 tgatgtacca ctgagccgcc cacagcacgt gcaccttggt caggttggcg gcaagcatcc
     3061 agcggcgcag ccggctcaac ggcccgggcg cgaaggtcgc gcgccaggcg tagggcacgt
     3121 cgctcgatcc ccaggcgtgg cgcacggact gggtgtagtg cgcgcgcagc gtcgtccggt
     3181 agccgtccgc cagcacgcag tcattcccga gcggcaggta cagcggttcg acgtgcacgc
     3241 ggtcgccgag ggcgaagcag cacttcaggt agacgtgcca gtcctccggg atgacttcct
     3301 cgtcccagta gtccacctcg gtcagcattt gccagctcag cgcgtagcag gaggtgggga
     3361 acttcacact catcggcagc acgaggttcg cgagccggtt aaccccggag aggccgtccg
     3421 ggagccgcag cggcgccggg atgtcccaga tgttgttgct gttgaagatc gcgggctgcc
     3481 agaaagcgcg atagcggtcc ttcgaggtca ggaacaggta gttcagcgcc tcgaagtggt
     3541 gcggatcgaa gaaggcgtcg gcatcacagc tcgtcaccgt gtaccgcccg aggtcgccgt
     3601 gaccggcctc gatcagccgc atgtgcgcct gccgggctgc ccacgcctcg tttgacccct
     3661 tgcccggcgt ctcccccggg atgccggccg gatggaacgt cgcgaagatg tcgccaaagc
     3721 gcgcgcggta ggccagcagt agccgcgccg ccttgcgctg ggccccggcc tcccgctcct
     3781 ccatcgccag ggccaccacg agctgccctg cgttcgcctg tgccgccagc gagtcgagcg
     3841 tgcgcgcgag ttcgtgctcg ttttcgcggt agttcgggat gatcaccagg tgacgtggcc
     3901 agtcccaggc ttcagcactc gagttgcgct cgcgccactc ggcatacttc gccggccact
     3961 ccgtccgctt ccagcggttg atgcggcgca gcccgatcca gcaggcggcg gcaactgagt
     4021 acgaccgccc gacccagtac acgtagaagc cgaccaggga gacggcaaag accgtcgcgg
     4081 tcagggagtg gaacggcgcc cagaagatga aggtgatcag gagcagtgac agcgtcagcg
     4141 ggattgcgtc gccaatgcgc tcgcgtaccc ggctgcgcga gtgcagccga gggatatgcg
     4201 gagacgacgc cgagggctcg ccgggcagcg ggtagcggac gatggccgca ctgggccgcg
     4261 cctcaatggc catagggaac ccctcgcacg aggttcttcc tacgcctgcg gggttagctg
     4321 acgggctacg gcggaagaag ccgctcccca ggtggttgcc ctacggcccc gcccgaggat
     4381 tcgccccgat ctctgggtcc cccactctcc cgagcgaccg ggagatttgg cgtgccacac
     4441 gctgcacgta tggtggctgt ccgccgtcag taagtcaacg gaagctcggc acccgcgagt
     4501 atcggaatca ttgcagcctg ttcacatcgc cgccgcctca gcgggccagc gcaaaagcag
     4561 agcccggccg aagccgggct cgctgtgagc gtcaactggc gtagccaccc gtgctacgag
     4621 gctggacgcc tcctatcgac ggccgtagcc gcgctggccg ccgcggtctc cgtacccgcc
     4681 gccgcggtcg ccgtaaccgc cacggtcgcg gtcgccgtag ccaccgccac cgtaaccacc
     4741 gcttccgccg ccgccgtacc cgccgcggtc acggtcgcca tagccaccgc caccgccacc
     4801 accgccatag ccaccgccgt aaccgccgcc gccaccgccg tagcggccac cgccgccacc
     4861 accaccgccc cgaggacggt cctcacgctc gcgcgcctga ttgacgttca gtgcgcgtcc
     4921 accgaagtcc ttgccgttga gcgcgtcgat cgccttcgtg gcgtcctcgc cggacatctc
     4981 cacgaaccca aaaccgcgcg gccgtcccgt ctcacggtcc gtcggcagta cgaccgagat
     5041 cacctcgccg tactgcgcga acagctcgcg cacgtcgtcc tcccgcgaac tgaacgggag
     5101 gttccccaca tagatcctct gattcaagaa ccactctcca cgtcggccct gcgccgacaa
     5161 ctgacccgcc tcaacgggcc tacgactaga ctctcaggct cgacgcggac gctcgggtgg
     5221 taacggccag gtagcgactg agaagacgcg gttccccgcg cccaaccctc attctacatc
     5281 tgcgccatcg cgtgtgtggt gtgggttggc ggcagcgccc tcccgcgcca tagactggaa
     5341 tgaccccgcc cttccgcacc gacgaggaac ctcgatgccc tttcgtgagg acatccgcaa
     5401 cgtcgcgatc atcgcgcacg tggaccacgg caagacgacg ctcgtggacg ccatgcttcg
     5461 ccagtccggc tcgttcgccg cgcaccagga agtagccgag cgcgtcctcg actcgatgga
     5521 tctcgagcgc gaaaagggca tcacgatcct cgccaagaac accgcggtcc gctacgacgg
     5581 cgtcaagatc aacatcatcg acacccccgg ccatgccgat ttcggcggcg aggtcgagcg
     5641 cggcctcacg atggtcgacg ccgcactcct gctcgtcgat gccagcgagg ggcccttgcc
     5701 ccagacccgc ttcgtgctcc gcaaggcact cgaggcgaac ctcgcgatca tcttggtcgt
     5761 gaacaagatc gaccgccccg acgcccgcat caaagaggtc gtcgacgagg tctatgagct
     5821 gttcctcgac ctcgacgcaa ccgaagcgca gatcgacttc cccatcgtct actgcaatgc
     5881 ccgcgccggc gaagcctcgc tcgaccccgc gcagccaggc gccgacctca agcccctgtt
     5941 cgacctgctg ctgaactacg tcgcgcctcc gagctacgag gacgaccacc ccctgcaagc
     6001 gctggtcacg aacctcgacg cttcgctcta cgtcggccgc ctcgcgctgt gccgtgtcat
     6061 ccaggggacg atccgccgag gtcagctggt cgcgcgctgc cgcgtcgacg gcacggtcga
     6121 gcgggtccgc gtcaccgagc tctacgtcac ggaagccctc gaacgcgtgc cggccgagga
     6181 ggccggcccc ggcgacatca tcgccgtggc gggcttccct gacgtcacca tcggcgagac
     6241 tctcgccgac gcggacgacc cccggccgct gccggtgatc cgcatcgatg aacccagcct
     6301 gtcgatgacc atcggcatca actcgtctcc gcttgccggg caggacggca acaagctcac
     6361 cgcgcgcctg ctcaagaacc ggctcgacca ggagcaggtg ggcaacgtct cgatccgcat
     6421 tctgcccgcc gaccgtcccg acacctggac ggtgcagggc cgcggcgagc tgcagctcgc
     6481 ggtcctcgtc gagaccatgc gccgcgaggg cttcgagctg accgtcggca agccggaggt
     6541 ggtcacacag gtgatcgatg gtcgcctctc cgagcctgtg gagcggctcg cggtggacgt
     6601 cccggaggac tacctcggcg tggtgaccca gctgctggcg ctgcgcaagg gccgtctcga
     6661 gcagatggtc aaccacggca gcggacgcgt tcgcatggaa tacctcgtcc cggcccgcgc
     6721 cctcatcggc ttccacaccg aattcctcac cgagacccgg ggcaccggac tgctgcatca
     6781 cgtcttcgac cgctacgagc cgtggcacgg cgaactgcgc accaggccaa ccggcagcat
     6841 ggtcgccgac cgccgtggcc ccaccacgac gtacgcgctc ctcagcttgc aggagcgtgg
     6901 ccagatgttt gtcggacccg gcatcgagac ctatgagggc atgatcgtcg gcgagaacgc
     6961 gcgggccgat gacatggacg tgaatcccac gcgtgagaag aagctcacga acatgcgctc
     7021 atcgacctcc gaggagaccg agaagctcgt accgcaccgg cagctctcgc tcgagcaggc
     7081 gctcgagttc atccgtgagg atgagtgctt ggaggtgacc ccaagccacg tccgcattcg
     7141 caaggttgcc ctggacggaa ttgagcgcgc ccgccgccgc tccgcccgcc gcgcctgacg
     7201 aatcgataac aaagcccgcg ttccgttccc tcggggcttc cgccttacgg gtagccgcgg
     7261 ctaaggtgag agacggcggg cctggcagtt gaccatctga agagatgcgc ccgtctatgt
     7321 ctgcgtcccg gactacgccc gttcgacggc ccgcacctga aacccccgct gacgcgacct
     7381 cacgccgcat gttcatggcg tttttcctcg ccgccatcgt ggtcgcggtc atctccttca
     7441 cgttctttct ccgcgatggt gccttgcaca gcctcggcat cggcggcggc aagtcgaacg
     7501 accgcagcgc aagtgcgctc ctcgtcaaag accccaacat tccgacgatc aaggacctga
     7561 accagctgcg ccagcaatac ggcgatcccg ctgatgcgac ctacggccgc atcctgatcc
     7621 cctcgattca cctcgacgcg cccatcggcg cgcggatcgt gggcgccgat gggcagatgc
     7681 ccgacccgac cgggccgagc gacgtcgtgt ggtacgactt ccagcacatg cccggctttg
     7741 gcggtgtccc cggaactgga cataatgcta tcttcgcggg ccacgtggat cgggcggcgt
     7801 atctcgacta cgccggggtc aactacatcg gtgccggcat cttctacagc ctgagcgagc
     7861 tggaccgcgg cgacaccatc accatcgagg tcggcgggaa gtcgtaccgc tacatcgtcg
     7921 cctgggtcca ggacgtgaag accgaatccg agtgggg
//
LOCUS       DAORPG010000008         7694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_407_length_7694_cov_3.727320, whole genome shotgun sequence.
ACCESSION   DAORPG010000008 DAORPG010000000
VERSION     DAORPG010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 7694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7694
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_407_length_7694_cov_3.727320"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLX85_00415"
     CDS             <1..298
                     /locus_tag="PLX85_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HRC61668.1"
                     /translation="ARAIAVEPEVVLMDEPCSALDPIATLKIEDLMYGLAERFTIVIV
                     THNLQQAARVSDWTGFMLAGEDGVGRLVEYRPTQDMFTNPREDRTEAYITGRFG"
     gene            315..980
                     /gene="phoU"
                     /locus_tag="PLX85_00420"
     CDS             315..980
                     /gene="phoU"
                     /locus_tag="PLX85_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRC61669.1"
                     /translation="MSREQFHKGLQGLQDGVLEMGSMVDRQIERSMQALINRDVPLAE
                     AVIRDDVEVNRSRFHLDNICLSLLAMQAPMASDLRLIVSVLSMITDLERMGDHAEGIA
                     RIVLLMRDEPLVKPLVDIPAMANTGRVMLKEVLDALVQRDEEAAYRVGAMDDEVDVLY
                     ERVYHDLIGIMIKDQSTVEACTHLLWIAHNLERIADRCTNIAERVVFTKTGMLPEMDI
                     STY"
     gene            complement(977..2254)
                     /locus_tag="PLX85_00425"
     CDS             complement(977..2254)
                     /locus_tag="PLX85_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HRC61670.1"
                     /translation="MTDVAPGGGISAAERRRAEAWPAMVLLVALTAMAPLSIDMFLPS
                     MPTISDEFGAEKATLQLAVTLFIICFAGSQLFYGPFSDRHGRRPTLVIGICLFVVGSL
                     VALTAQSVGMLLAGRVLQGLGGGAGPAIGQAIVLDIYGRERAGKVIAYMAIALPLAPA
                     VAPIIGGVLHDAWGWHAVFVTLAVLGTVLLTAYLALLPETNRREAGAGRGSNGLLADY
                     RTLLSNRTFSTYALIMGLMFGGQLVFISSSSFVLIDELGLSARLYGLSFGFVAIGIMG
                     GATLSARLVRRGWTHQAVLSGVTTAATAATVLAVLSWLGVHHVAAVLLPMFVVALGLG
                     MTRAPAMASALVPFTTMAGLASATLGFSQMLIASSYNITYSRFVEASTTALATGVFLS
                     IAASLLVVLVLRPGRSMPAATVATAVPVAEAER"
     gene            complement(2247..2840)
                     /locus_tag="PLX85_00430"
     CDS             complement(2247..2840)
                     /locus_tag="PLX85_00430"
                     /inference="COORDINATES: protein motif:HMM:NF025244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4202 domain-containing protein"
                     /protein_id="HRC61671.1"
                     /translation="MNEVDRFRRATELIDAANAQDPNRLETGAGPRPKELVHAELLTE
                     WIRRLEPEPSEALLLAARAHHIRRWVVPRSSYPPGRASYLQWRRDLHRFHADEVGAIL
                     ERVGYDAGTITRVQQIVRKERLTLDPEVQTLEDGLCLVFLQLQLDSTAESVGDEGRMV
                     EVLRKSWRKMSPAGREFALRLELSDEGRSLVRRALGD"
     gene            3002..4786
                     /locus_tag="PLX85_00435"
     CDS             3002..4786
                     /locus_tag="PLX85_00435"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HRC61672.1"
                     /translation="MTTTRTDAPGRVIPILQQEFEDFRTEATAFLEGKTEEAIFIGFR
                     LKQGVYGQRQPARQMIRVKLPFGAVSADQLDAFGRVAEEYAPLRKGHITTRQNYQFHH
                     IPLERMTEVLEYLGTVGLSSREACGNTVRNVTGDPYAGVRPDEPFDISPYAGAFVRYF
                     VRQNLTQLLPRKCKVAFTGSAQDEAITDIHDIGFIPEIRDVDGVPTKGFKMVAGGGLA
                     IMARHAVTIREWVSVDDYLRVSEAVIRIFNAADELRKNRAKARLKFLVHRVGEAEFNR
                     MVDDELMKPWADQRISPEDLLFLDDEEADAPPHPENPKQPGADRAEFERFWTTNVRKQ
                     RQAGYCTIDVTVPQGDMSPEQFRGVASILRTYGNGRARTSVNQNIVLRWIPEGSVYAV
                     WTELQALGLAKGGSGEITDVVSCPGTDSCKLGITCSMGLNRAISSKLYEMNIEDPTTR
                     KMRINMSGCPNSCGQHHLASIGFHGAAIKASNDADRQVPAYHIFMGGARGDGHIRIGQ
                     LLKARIPAKRAPVVVERFIRYYEAERQSPDEEFNAFFDRVGPAPFDALLGDLTIPPEF
                     SLDNMGEFIDWERDGLYVLERGEGECAI"
     gene            4828..5559
                     /locus_tag="PLX85_00440"
     CDS             4828..5559
                     /locus_tag="PLX85_00440"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HRC61673.1"
                     /translation="MTTETKATEADPRELAAIEEASIALDQAEPEEILRWAIDRYGEG
                     LTLACSFGGISGMVLMDMVQNLAPSTEVFYLDTDYLFPETYEMVDTAKAKWVGANIVG
                     YRTDVSTEEQARIHGANLWERDPDLCCEIRKVEPNRRALDGKAAWISGLRRDQSTDRG
                     STPAVQWDAKFGLVKVNPLINWDEKKVWAYIFKHSVPYNPLHDRGYPSIGCTNCTKPV
                     KPGDDPRSGRWSGMDKDECGLHTAP"
     gene            5642..6187
                     /gene="cysC"
                     /locus_tag="PLX85_00445"
     CDS             5642..6187
                     /gene="cysC"
                     /locus_tag="PLX85_00445"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006456990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HRC61674.1"
                     /translation="MGQQGFVVWFTGLSGAGKSTLAEHLAPILRERGVPVEILDGDEV
                     RTNLSKGLGFSKEDRDTNILRIGYVAKLLARNGVCVITAAISPYADIRQQVRDSVDNF
                     IEVHVDCSIDELSRRDVKGLYAKALRGEIANFTGVSDPYEAPETPEVRVNSEHQTEQE
                     SLNDILNYLEQRGLVPVAARA"
     gene            6180..7349
                     /gene="sat"
                     /locus_tag="PLX85_00450"
     CDS             6180..7349
                     /gene="sat"
                     /locus_tag="PLX85_00450"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HRC61675.1"
                     /translation="MPELIAPHGGRLNERLVTGDAAQALAERARSLKTVTLDARQVTD
                     LELLAVGALSPLDGFMGKADYDRVIDEMHLASGAVWSVPVTLGVPAEEARALVGSEVA
                     LVTPDGTPLAVMQVAEAFDADRPREALKVYRTDDMAHPGVEAIFAQGNTLIGGSVQVF
                     ALPPDPEFPEYRLTPAQTRAYFEAQGWRTVVGFQTRNPVHRAHEYLQKVALETIDGLL
                     LHPLVGETKEGDIPADVRMRCYRVLLDNYYPKDRVLLSVLPASMRYAGPREAIFHAIM
                     RQNYGCSHFIVGRDHAGVGDYYGTYDAQTIFDELAPGELGITPLKFEHTFFCRACDSM
                     ASTRTCPHTSDHHVVLSGTKVRQMLAAGEVPPVEFSRPEVAQVLIDSMRTETASV"
     gene            7388..>7694
                     /locus_tag="PLX85_00455"
     CDS             7388..>7694
                     /locus_tag="PLX85_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61676.1"
                     /translation="MSHRLVSFLPGRLHRPTLITATTIRLLVGGALVFYLFVLALRLL
                     SASASGVAHLLTQIGADGPLNLLGFGWLLAYGALSGSPVAALSLSLLHGGAISTSEAL
                     "
BASE COUNT         1355 a         2872 c         2332 g         1135 t
ORIGIN      
        1 tgcccgcgcc atcgcggtgg agcccgaggt cgtcctgatg gacgagccct gctcagccct
       61 cgacccgatc gccaccctca agatcgaaga cctcatgtac ggcctcgccg agcgcttcac
      121 catcgtgatc gtcacgcaca accttcagca ggccgcccgc gtcagcgatt ggaccggctt
      181 catgctcgcc ggcgaagacg gggtgggccg actcgtcgag taccgcccta cgcaggacat
      241 gttcaccaac ccgcgggagg accgcaccga ggcctacatc accggccggt tcggctaggc
      301 ggagggggag agtcatgtca cgcgagcaat tccacaaggg cttgcaggga ctccaggacg
      361 gcgtccttga gatgggctcg atggtcgacc gtcagatcga gcgctcaatg caggcgctca
      421 tcaaccgcga cgtacccctc gcggaggccg tcatccggga cgacgtcgag gtgaaccgct
      481 cacgcttcca cctcgacaac atctgcctct cgctcctcgc catgcaggcc ccgatggcaa
      541 gcgacctccg cctgatcgtc tccgtgctct cgatgatcac cgacctcgag cgcatgggag
      601 accacgccga gggcatcgcg cgcatcgtct tgctgatgcg cgacgaaccg ctcgtgaagc
      661 cgctcgtgga catcccggcc atggccaaca cgggccgcgt catgctcaag gaggtcctcg
      721 atgcgctcgt acagcgtgac gaggaggccg cctaccgcgt cggcgccatg gacgacgagg
      781 tggacgtcct ctacgagcgc gtctaccacg acctcattgg catcatgatc aaagaccagt
      841 cgacggtgga ggcctgcaca cacctgctct ggatcgcgca caacctcgag cgcatcgccg
      901 accgctgcac caacatcgcc gagcgcgtcg tcttcaccaa gaccggcatg ctgccggaga
      961 tggacatctc gacctactag cgctcggcct ccgccaccgg gaccgcggtc gcgaccgtcg
     1021 ctgctggcat cgaccgcccc ggacgcagta cgagcaccac gaggaggctc gctgcaatcg
     1081 agaggaagac gccggtcgcc agggccgtcg tgctcgcctc gacgaagcgg ctgtacgtga
     1141 tgttgtagct cgaggcgatc agcatctggc tgaacccgag cgtcgccgag gccagccccg
     1201 ccatcgtggt gaacggcacc agcgccgagg ccatggcggg cgcccgcgtc atccccagcc
     1261 ccagcgccac cacgaacatc ggcagcaaca ccgccgccac gtggtgcacc ccgagccagg
     1321 agagcaccgc cagcacggtc gccgccgtcg cggccgtggt cactccgctc aatacagcct
     1381 ggtgcgtcca ccctcgccgc acgaggcgcg ccgagagcgt ggcgccgccc atgattccga
     1441 tcgcaacgaa cccgaagctc agcccgtaca gccgcgcgct caaccccagc tcatcgatca
     1501 gcacgaacga ggacgacgag atgaagacga gctggccgcc gaacatcagc cccatgatca
     1561 gcgcgtacgt cgagaacgtg cggttcgaca gcagcgtccg gtaatccgcg agcagtccgt
     1621 tcgatcctcg tccggcaccg gcctcccggc ggttcgtctc cggcagcagc gcgaggtacg
     1681 ccgtgagcag cacggtgcca agtaccgcca gcgtcacgaa cacggcatgc cagccccagg
     1741 cgtcgtgcag gaccccgccg atgatcggcg ccacagcggg cgccagcggc aacgcgatcg
     1801 ccatgtacgc gatcaccttg cccgcccgct ctcgtccgta gatatcgagg acgatcgcct
     1861 gcccgatcgc cggccccgca ccgccgccca gcccctgtag cacccgcccg gcgagcagca
     1921 tcccgaccga ttgcgccgtc agcgccacca ggctgccgac gacgaacagg caaatgccga
     1981 tgacgagcgt gggccggcgc ccgtgccggt ccgagaatgg cccgtagaac agctgcgacc
     2041 ccgcgaaaca gatgatgaag agcgtaacgg cgagctgcag cgtcgccttc tcggcgccga
     2101 actcatcaga gatcgtgggc atcgagggca ggaacatgtc gatcgacagc ggcgccatcg
     2161 cggtaagcgc caccagcagc accatggccg gccacgcctc ggcccgccgc cgctcggcgg
     2221 ccgagatgcc cccacccggt gccacgtcag tcaccgagcg ctcgtcgcac caggctccgc
     2281 ccctcgtcac tcagctccag gcggagcgcg aactcgcgcc cggccggcga catcttccgc
     2341 cacgacttcc gaagcacctc gaccatccgt ccctcgtccc ccacgctttc ggccgtcgaa
     2401 tcgagctgca attgcaggaa gacgaggcag agcccgtctt cgagcgtctg cacctccggg
     2461 tcgagcgtca gccgttcctt gcgcacgatc tgctgcacgc gcgtaatcgt gcccgcatcg
     2521 tagcccaccc gctcgaggat cgcgcccact tcatcagcgt gaaaccggtg gaggtctcgg
     2581 cgccactgca ggtacgacgc tcgccccggc gggtacgagg agcgcggcac cacccaccgc
     2641 cggatatggt gcgcgcgggc ggccagcaac aacgcctcgc tcggctcggg ctcaagccgc
     2701 cggatccact ccgtcagcaa ctctgcgtgc acgagttcct tgggccgcgg ccccgcgcct
     2761 gtttcgaggc gattcggatc ctgggcgttg gctgcgtcga tcagctcggt tgcccggcgg
     2821 aagcgatcaa cctcgttcat ttccgcccgc cccacgctcg ccgtcagaac aattagactg
     2881 cgtttgcttc tctattgaga cgcctcatgt tccgagtgat agcatagctc cattatcgtc
     2941 aaacttgacc atgttactca acattggcga tgatagagcc caccagcgag ggggactggc
     3001 catgaccacc acacgaaccg acgctcccgg ccgcgtcatc cccatcctcc agcaggagtt
     3061 tgaggacttc cgtaccgagg ccaccgcatt cctcgagggg aagaccgagg aggccatctt
     3121 catcggcttc cgcctcaagc agggggtgta cggccagcga caacccgcac gccagatgat
     3181 ccgcgtgaag ttgccgttcg gggccgtcag cgccgatcag ctcgacgcct tcgggcgcgt
     3241 cgccgaggag tatgcgcccc ttcgcaaggg ccacatcacc acccggcaga actaccagtt
     3301 ccaccacatc ccgctggagc gcatgaccga agtgcttgag tacctcggca ccgtcggcct
     3361 ctcgagccgc gaggcgtgcg ggaacacggt acgcaacgtc acgggcgacc cgtacgccgg
     3421 cgtccgcccg gacgagccgt tcgacatttc gccgtatgcc ggcgctttcg tgcgctactt
     3481 cgtacgccag aacctgacgc agctgctgcc ccgcaagtgc aaggtcgcct tcaccggcag
     3541 cgctcaggac gaggcgatca ccgacatcca tgacatcggg ttcatccccg agatccgcga
     3601 cgtcgatggc gtccccacca agggcttcaa gatggtggcc ggcggtggcc tcgcgatcat
     3661 ggcccgccac gccgtcacca tccgcgaatg ggtctcggtc gatgactacc tgcgcgtctc
     3721 cgaggccgtc atccgcatct tcaacgcggc ggatgagctg cggaagaacc gcgccaaggc
     3781 ccgcctcaag ttcctcgtgc accgcgtcgg cgaggccgag ttcaaccgca tggtggacga
     3841 cgagctgatg aagccctggg ccgaccagcg catcagcccc gaagacctcc tcttcctcga
     3901 cgacgaggaa gccgacgctc cgccacatcc cgagaacccg aagcagcccg gcgccgaccg
     3961 cgccgagttc gagcgcttct ggaccaccaa cgtccgcaag cagcgccagg ccggctactg
     4021 caccatcgat gtcaccgtcc cgcagggcga catgtcgccg gagcagttcc gaggcgtcgc
     4081 gagcatcctg cgcacctacg gcaacggccg cgcacgcacg tcggtgaacc agaacatcgt
     4141 gctccgttgg atccccgagg gcagcgtcta cgccgtctgg accgagctgc aggcactcgg
     4201 cctcgcgaag ggcggctcgg gcgagatcac ggatgtcgtc tcctgccctg gcaccgactc
     4261 ctgcaagctc ggcatcacct gctccatggg cctcaaccgc gccatctcct cgaagctcta
     4321 cgaaatgaac atcgaggatc cgacgacccg gaagatgcgc atcaacatga gcggctgccc
     4381 caactcctgc ggccagcacc acctcgcgag catcggcttc cacggtgcgg ctatcaaagc
     4441 ctcgaacgac gccgaccgcc aggtccccgc gtaccacatc ttcatgggcg gcgcccgcgg
     4501 cgatggccac atccgcatcg gccagctgct gaaggcgcgc atccccgcga agcgcgcgcc
     4561 cgtcgtcgtc gagcgcttca ttcgttacta cgaggccgag cgccagagcc cggacgagga
     4621 gttcaacgcg ttctttgacc gcgtcggacc cgccccgttc gatgccctcc tcggcgacct
     4681 caccattccg cccgagttct cgctcgacaa catgggcgag ttcatcgact gggagcgcga
     4741 cggactctac gtcctcgaac gcggcgaagg cgagtgcgcg atctagcgca caacgacagc
     4801 agggatcagc aagacagggc agcgaccatg accacagaga cgaaggccac cgaggcggat
     4861 ccgcgcgagc tcgccgcgat cgaggaggca tcgatcgccc tcgatcaggc ggagcccgag
     4921 gagatcctgc gctgggcgat cgaccgctac ggcgagggcc tcactctcgc ctgctccttc
     4981 ggcggcatct ccggcatggt cctcatggac atggtccaga acctggcccc gtcgaccgag
     5041 gtgttctacc tcgacaccga ctacctgttc cccgagacct atgagatggt cgacaccgcg
     5101 aaggcgaagt gggtcggcgc gaacatcgtc ggctaccgca cggacgtctc caccgaggag
     5161 caggcgcgca tccacggcgc gaacctgtgg gagcgcgacc ccgacctctg ctgcgagatc
     5221 cgcaaagtcg agccgaaccg gcgcgccctc gatggcaagg cggcctggat ctcgggcctc
     5281 cgccgcgacc agtccaccga ccgtggctcg acgccggccg tccagtggga cgccaagttc
     5341 ggcctcgtga aggtcaaccc gctcatcaac tgggacgaga agaaggtctg ggcctacatc
     5401 ttcaagcaca gcgtccccta caacccgctg cacgaccgcg gctacccgag catcggctgc
     5461 accaactgca ccaagcccgt gaagcccggc gacgacccgc gctcgggccg ctggtccggc
     5521 atggacaagg acgagtgcgg gctgcacacc gcgccctagc gcatcgaggc tgattccgcg
     5581 gcgccccacc cgggcgccgc gtgaccaaac gcgcaacgac gtcacatcgg aagggatcaa
     5641 cgtggggcaa cagggatttg tcgtgtggtt caccgggctg tcaggcgccg gtaagtcaac
     5701 gctcgccgag catctcgcgc ccatcctgcg cgagcgggga gtcccggtcg aaatcctcga
     5761 cggtgacgag gtgcgcacca acctctcgaa gggcctcggc ttctcgaagg aagaccgcga
     5821 caccaacatc ctgcgcatcg gctacgtcgc gaagctcctc gcccgcaacg gcgtctgcgt
     5881 catcaccgcc gccatctcgc cctacgccga catccgccag caggtccgcg actccgtcga
     5941 caacttcatc gaggtgcacg tcgactgctc gatcgacgag ctgtcgcgcc gcgacgtgaa
     6001 gggcctctac gccaaggccc tgcgcggcga gatcgccaac ttcaccggcg tctccgaccc
     6061 gtacgaggcc ccggagaccc ccgaggtgcg cgtgaactcc gagcaccaga cggagcagga
     6121 gtcgctcaac gacatcctca actacctcga gcagcgcgga ctcgtcccgg tggccgcccg
     6181 tgcctgagct catcgcgccc cacggcgggc ggctcaacga acgcctcgtc actggcgatg
     6241 ccgcccaggc cctcgccgag cgcgcccgct cgctcaaaac cgtcaccctc gacgcccgcc
     6301 aggtcaccga cctcgagctg ctcgcggtcg gcgcgctcag ccccctcgat ggcttcatgg
     6361 gcaaggctga ctacgaccgt gtcatcgacg agatgcacct cgccagcggc gcggtgtgga
     6421 gcgtccccgt caccctcggc gtccccgccg aggaggcgcg cgcactcgtc ggctccgagg
     6481 tcgcgctcgt cacgccggac ggcacgcccc tcgccgtcat gcaggtcgcc gaggcgttcg
     6541 acgccgaccg cccgcgcgag gcgctcaagg tctaccgcac ggacgacatg gcccaccccg
     6601 gcgtcgaggc aatcttcgcc cagggcaaca cgctcatcgg cggctcggtg caggtcttcg
     6661 ctctgccgcc cgaccccgag ttcccggagt accgcctcac gcccgcgcag acacgtgcct
     6721 acttcgaggc gcagggctgg cgcacggtcg tcggcttcca gacccgcaac cccgtccacc
     6781 gtgcgcacga gtacctccag aaggtcgccc tcgagaccat cgatggactc ctgctgcacc
     6841 ccctcgtcgg cgagacgaag gaaggcgaca tccccgccga cgtccgcatg cgctgctacc
     6901 gggtactgct cgacaactac tacccgaagg accgcgtgct gctctcggtg cttcccgcct
     6961 cgatgcgcta cgcgggcccg agggaggcga tcttccacgc gatcatgcgc cagaattacg
     7021 gctgctcgca cttcattgtg ggccgcgacc acgccggcgt cggcgactac tacggcacct
     7081 acgatgcgca gacgatcttc gatgagctgg cgcccggcga gctgggcatc acgccgctga
     7141 agttcgagca caccttcttc tgccgcgcct gcgactcgat ggcgagcaca cgcacctgcc
     7201 cgcacacctc ggatcaccac gtcgtgctct cgggcacgaa ggtccgtcag atgctcgcgg
     7261 ccggcgaggt accccctgtc gagttctcgc gccccgaggt cgcccaggtg ctgatcgact
     7321 cgatgcgcac cgagaccgcg agcgtctgaa cgtggcggac gccccgccaa ccggggccgc
     7381 tcggccgttg agccaccgcc tggtgtcgtt cctcccggga cggttgcacc ggcccacgct
     7441 catcacggcg accacgatcc gcctcctcgt cggaggcgcg ctcgtgttct acctcttcgt
     7501 gctcgcgctg cggctgcttt ccgcctccgc gagtggggtc gcgcatctgc tgacgcagat
     7561 cggcgcggac ggaccgctga atctcctcgg gttcggctgg ctgctcgcct acggcgcgct
     7621 ctcgggctcc cccgtcgccg ccctctcgct ctcgctactg cacggtggcg cgatcagcac
     7681 ctccgaggcg ctcg
//
LOCUS       DAORPG010000009         7624 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_416_length_7624_cov_3.344960, whole genome shotgun sequence.
ACCESSION   DAORPG010000009 DAORPG010000000
VERSION     DAORPG010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 7624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7624
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_416_length_7624_cov_3.344960"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PLX85_00460"
     CDS             <1..241
                     /locus_tag="PLX85_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRC61677.1"
                     /translation="NKVVPPDQLMDSAMALAQEIAGNPPLTVRASKQLIHRRQRLDDA
                     LPYEHDANGPSMPSDDRIEALRAFVEKRDPVYYGR"
     gene            297..1505
                     /locus_tag="PLX85_00465"
     CDS             297..1505
                     /locus_tag="PLX85_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRC61678.1"
                     /translation="MSGPLEGWRVLDLTQGIAGPYVTKLCSDYGADVLKVERPGVGDA
                     SRNIGPFPGDHPNVDASGMFLTLNTGKHSVTLNLKTKTGREILLRLAKEADLVVESFR
                     AGTLERLDLAPERFAEANPSAALVRISNFGQTGPMRDFSADDLTAYAAAGILQVTGEP
                     DREPVGLGIYTPLFLAGAIVAPMALAAMRAAHRDGQQEVVDFSIQEGLAGSMDRGGTN
                     LVSFQYSGALMVERRERVLINSLPNGVFPCADGYIQVTTQVNWFDRFCRVIGRPEWIT
                     DPRMTSNLNNPLEIGGEVEAAFYEWLLPRTKQQAMEEAQAVGWPLSALNTPADVIRDP
                     HFRERGFFVGIDHPQAGHIELPGLAVRFSDTPGEVRRPPLLGEHTFEVLAGLGYAPQE
                     IAALRAESVI"
     gene            1516..2820
                     /locus_tag="PLX85_00470"
     CDS             1516..2820
                     /locus_tag="PLX85_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRC61679.1"
                     /translation="MSDRRPPLDGVRVLAQGLVWAGPFSTVILSDLGAEVIEVESIQH
                     LNPTRTQMRHIPDVVMESVGGTYYVNRDPSEGFWNRQAWFNYGKRGCKSVTLNLHSAR
                     GRELFLELVKQSDAFIENNAAGVVDSLGIGWDVLHATNPRLIMVRFPGFGITGPYSHH
                     KGYGSNMEAVAGHTMVRGYADADPSLTPASLHGDPNAGAHVAWAVQAALLARERTGGG
                     QLIEISQSEAVLHHIAYDILDYTLNGRETGQRGNNHPAFAPYGVFPCAGHDRWIAIAV
                     TSDEGFAALSEVIGAPELASDERFATTIDRLRHRSEVNAAVGERTGAMQNEQLAKALQ
                     ERGVTASALAHQQEMHEDEHLRSRGYFNSITHPEAGTHLYPGPLAKLERTAAVPPFQP
                     APTLGQHNEYVLKDLLGLDDAAYQQLIEEQVIGDTYLESATA"
     gene            2924..3694
                     /locus_tag="PLX85_00475"
     CDS             2924..3694
                     /locus_tag="PLX85_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRC61680.1"
                     /translation="MPDLLYEKKDFYAVFTMNRPERLNALGGTMNAERQAALDDFNSD
                     PKMRVGIVTGAGRAFCAGADLKEMAERNASGVAQNRRIVGSASMGYAASPKPFIAAIN
                     GLAIGGGFETSMDCDIRICSTEAYFGLFEPKRGILAGYGMHHLPRIIGLTHAAYILLT
                     ADRVSPEQAKEWGIVSEVTEPDRLLPRAVEIAEMIVANAPLSIEGTKAMLQAWRMAKI
                     DDSYRLGAWVSDRVLLSDDAKEGPKAFAEKRAPNWVDG"
     gene            3845..4375
                     /locus_tag="PLX85_00480"
     CDS             3845..4375
                     /locus_tag="PLX85_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HRC61681.1"
                     /translation="MTTEQPETLVTQEMIDTRDKWSEPRKSWPVSASDIRRWAQGVYW
                     PEVPPRLYWDEEYAKNTKWGGIIAPEEFNPFAFPLEGAYGQEAALAEVQGRPAPSTGT
                     MGSGPGSNAMNGGQTIRYHAPIRPGDVISTTSALVDWNERQTRLGLTMFVTNETRWTN
                     QNGELVCVKTQVSIRY"
     gene            4400..4834
                     /locus_tag="PLX85_00485"
     CDS             4400..4834
                     /locus_tag="PLX85_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61682.1"
                     /translation="MAYNVYFEDVNVGDELPGFKRRTDLMNWNRYAAINDEFIPIHMD
                     DAAGRQGQNEQGAFGMGNLRTAYIHNMLRNWAGDEAEIREVGIQFRAINQKNDQLTTT
                     GKIVEKYVDGNDHLVRIETNVVNQEGAGTAPGHAIICLPSRG"
     gene            4985..5602
                     /locus_tag="PLX85_00490"
     CDS             4985..5602
                     /locus_tag="PLX85_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61683.1"
                     /translation="MRTRVALLGGVAAAALLVAGVVGVAAQTGDSGSANPVSTFMDRL
                     ASNLGIGKDQLQAAVDKTRGQMLDDAVQQGRLTQDQADQLKQQSAGKGLGEGLGRFHS
                     GAGAEGNHDGQPFGRGGPGMRGLAGAGLDAVASAIGIDKATLMQELASGKSLAQVAQA
                     HGVSRDQLKQKLTDAFGSKLDELLDQTFQFGAHGGNPSATPTPSS"
     gene            complement(5658..6506)
                     /gene="folP"
                     /locus_tag="PLX85_00495"
     CDS             complement(5658..6506)
                     /gene="folP"
                     /locus_tag="PLX85_00495"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRC61684.1"
                     /translation="MLLQLGPHTIDCTNRTVVMGILNVSYDSPISESIVRPGQALERA
                     LELRDAGAEIIDIGAHSTRTGGRDLTAQEEVDRVCPAIEAIAAAGIPVSVDTWTPAVA
                     NEAARAGVHLLNDVTGFTNPAMVDVAVRCRLPVVAMHMRGAPKHHREADQTYDNIAHD
                     VEVFLTRQANALESAGVAGVWLDPGFGFGKSAADNLRLLNALPRMTSVGRPVLVSASR
                     KGFLAELLGAGDRQDVEGLIEATIAFNVLAAWSGVHVVRVHDVREVVRAIRVVNAARS
                     RLMERE"
     gene            complement(6510..7376)
                     /locus_tag="PLX85_00500"
     CDS             complement(6510..7376)
                     /locus_tag="PLX85_00500"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HRC61685.1"
                     /translation="MGADEPVRVGIFGAGRLGSALATALVAAGEQVKAVSSRTVRSAE
                     HLATALGAGVAVLAPQAVADSCDLVFLTVPDAAIAALASEIRWRQGQWVVHCSGALDL
                     GPLSGAVRQGALVGCLHPLQSFPSREGGAARFRGITCGVEGSNPLGRVLEELVGRLGA
                     SVVRLEGVDRAAYHAAAVFASNDVVALMAAAREAWTLAGLSAASARTSLVPLLLGAAQ
                     NVAEHDLRDALTGPVARGDVDTVRRHLAVLGDSELGALYRRLGAELLTLDLPHDAATS
                     SALRDAFAAGAG"
BASE COUNT         1365 a         2614 c         2554 g         1091 t
ORIGIN      
        1 aaacaaggtg gtcccgcccg accagctgat ggactccgcg atggccctgg cgcaggagat
       61 cgccggtaac ccgcccctga ccgtgcgcgc gagcaagcag ctcatccacc gccgccagcg
      121 cctcgacgac gcgctgccgt acgagcacga cgcgaacggc ccctcgatgc ccagcgacga
      181 ccgtatcgag gcgcttcgcg ccttcgtcga gaagcgcgac ccggtctact acggccggta
      241 acgatgagta gcgcgggagg cgggctggag cccgcctcca ctcaaggcgg cgccctatgt
      301 ccggtccgct cgaaggctgg cgcgtcctcg acctgactca ggggatcgct ggtccgtacg
      361 tcaccaagct ctgctccgac tacggcgctg acgtcctcaa ggtcgagcga ccgggagtgg
      421 gcgacgcgtc gaggaatatc ggccccttcc cgggcgacca cccgaacgtg gacgcgagcg
      481 ggatgttcct caccctcaat acgggaaagc acagcgtcac cctcaacctg aagacgaaga
      541 cgggtcgcga gatcctccta cggctggcga aggaggcgga tctcgtcgtc gagagcttcc
      601 gcgccgggac gctggagcgg ctcgacctcg ccccggagcg ttttgccgag gccaacccgt
      661 cggccgcgct cgtccgcatc tcgaacttcg gccagaccgg ccccatgcgc gacttcagcg
      721 ctgacgacct gaccgcttac gccgcagccg gcatcctgca ggtcaccggc gagcccgacc
      781 gcgaaccggt aggcctcggt atctatacgc cgctcttcct cgcgggggcg atcgtggcgc
      841 cgatggccct cgcggccatg cgagccgccc accgcgacgg acagcaggag gtcgtggatt
      901 tctcgatcca ggaggggctc gccggatcca tggaccgagg cggcacgaac ctcgtcagct
      961 tccagtacag cggggcgctc atggtcgagc gccgtgagcg cgtcctcatc aactccctgc
     1021 cgaatggagt cttcccctgc gccgacgggt acatccaggt caccacgcag gtgaactggt
     1081 tcgaccgctt ctgccgcgtc atcggtcgtc cggagtggat caccgatccg cgcatgacct
     1141 ccaacctcaa caacccgctc gaaatcggcg gtgaggtgga ggcggcgttt tacgagtggc
     1201 tgttgccgag gacgaagcag caagccatgg aagaggccca ggcggtcggc tggccccttt
     1261 cggcgctgaa tacgcccgcc gatgtcatcc gcgacccgca cttccgcgag cgcggcttct
     1321 tcgttggcat cgaccatcca caggccggcc acatcgagct gcccgggctc gccgtccgct
     1381 tcagcgacac ccccggcgag gtgcgccgcc cgcccttgct cggcgaacac accttcgaag
     1441 tgctggccgg cctcgggtac gcgccgcagg agatcgcggc gctacgcgcc gagagcgtca
     1501 tctgaggagg agtcgatgag cgaccgcagg cctccactgg acggcgtccg tgtcctcgca
     1561 cagggcctcg tctgggccgg gcccttctcg accgtgatcc tgtccgacct cggcgcagag
     1621 gtgatcgagg tggagtcgat ccagcacctc aaccccaccc gcacacagat gcgtcacatc
     1681 cctgacgtcg tcatggaaag cgtcggtggc acctactacg tgaaccgcga cccctccgag
     1741 ggcttctgga accgccaggc gtggttcaac tacggcaagc gcggctgcaa gtcggttacg
     1801 ttgaacctcc acagcgcccg tggccgtgag ctcttcctcg aactggtgaa gcagtcggac
     1861 gcgttcatcg agaataatgc cgcgggcgtg gtcgactccc tcggcatcgg ctgggacgta
     1921 ctccacgcca cgaacccgcg cctgatcatg gtgcgcttcc ccggtttcgg catcacgggc
     1981 ccctacagcc accacaaggg ctacggctcg aacatggagg ctgtcgccgg ccacacgatg
     2041 gtgcgcggct acgccgacgc cgacccctcg ctcacgcccg cttccctgca cggcgacccg
     2101 aacgcgggcg cacacgtcgc atgggcggtg caggcggcgc tcctggcccg tgagcgcaca
     2161 ggcgggggcc aattgatcga gatctcgcag tccgaggccg tgcttcacca catcgcctac
     2221 gacatcctcg actacaccct caacggccgc gagacggggc agcgagggaa caaccacccg
     2281 gccttcgcgc catacggggt gttcccctgc gcgggccacg accgctggat cgcgatcgca
     2341 gtgacctcgg atgaagggtt tgctgcgctg tccgaggtca tcggggctcc cgaactcgcc
     2401 tcggatgagc ggttcgccac gaccatcgac cgcctccgcc accgctccga ggtaaacgcg
     2461 gccgtcggcg agcggacagg cgcgatgcag aacgagcagc tcgcgaaggc gctgcaggag
     2521 cgcggcgtca ccgcatcggc actggcgcac caacaggaaa tgcatgagga cgagcacctc
     2581 cgttcgcgcg gctacttcaa ctcgatcacg caccccgagg ccgggacgca cctgtacccc
     2641 ggcccgctcg cgaagctcga gcgaacggcg gcggtgccgc cgttccagcc ggcgcccacg
     2701 ctcggtcaac acaacgagta tgtgctgaag gacctgctgg gcctcgatga cgcggcgtat
     2761 caacagctca tcgaggagca ggtcatcggg gacacgtacc tcgaaagcgc gactgcctga
     2821 cgaggcgcgg gcgcgcggag attggttggg ctcaagcgtc ccactgcgcc atcggcggcg
     2881 tagtatgacg ctcggcatgc aagggactta gaggaggaaa cagatgccag acctgctgta
     2941 cgagaagaag gacttttacg ccgtcttcac gatgaaccga ccggagcgcc tgaacgcgct
     3001 cggtgggacg atgaacgccg agcgtcaggc cgcgctcgac gacttcaact cggacccgaa
     3061 gatgcgcgtg ggcatcgtca cgggtgcggg ccgggcgttc tgcgccggcg ccgacctgaa
     3121 ggagatggcc gagcgcaacg cctcgggcgt cgcccagaac cgccgcatcg tgggctccgc
     3181 ctcgatgggc tacgcggcca gccccaagcc gttcattgcg gccatcaacg gcctcgccat
     3241 cggcggcggc ttcgagacct cgatggactg cgacatccgc atttgctcga ccgaggccta
     3301 cttcggcctg ttcgagccga agcgcggcat cctcgccggc tacggcatgc accacctccc
     3361 gcgcatcatc ggcctgacgc acgccgccta cattctgctg acggccgacc gtgtgagccc
     3421 tgagcaggcg aaggaatggg gcatcgtcag cgaggtcacc gagcccgacc ggctgctccc
     3481 gcgtgccgtc gagatcgccg agatgatcgt cgcgaacgcc ccgctgtcca tcgagggcac
     3541 aaaggcgatg ctccaggcct ggcgcatggc gaagatcgac gactcgtacc gcctcggcgc
     3601 ctgggtgtcc gaccgcgtcc tgctgtccga cgacgccaag gagggcccga aggcgttcgc
     3661 cgagaagcgc gcccccaact gggtcgacgg ctagtccgtc ctcgcctcgt acgtcacgag
     3721 gtcacgaacg ctgctccggg atcccggagc agcgttcgtc gtctacccgg aacccgcagc
     3781 cccttcccgc acgaacgagg gttgctatca tcgcgtacgg catcaagcaa cggagaggaa
     3841 gacgatgacg acagagcagc ccgagacgct cgtaacccag gagatgatcg acacccgcga
     3901 caagtggagc gagcctcgga agtcgtggcc ggtgtcggcc tcggacatcc gccgctgggc
     3961 gcagggggtg tactggccgg aggtcccgcc ccgtctctac tgggacgagg agtacgcgaa
     4021 gaacacgaag tggggcggca tcatcgctcc ggaggaattc aacccgttcg cgttcccgct
     4081 ggaaggcgcg tacggccagg aggcggccct cgccgaggtg caagggcgtc cggctccctc
     4141 gaccggcacg atgggtagcg gccccggctc caacgccatg aacggcggcc agacgatccg
     4201 ctaccacgct ccgatccgcc cgggtgacgt gatctcgacc acgagcgcgc tggtggactg
     4261 gaacgagcgc cagacgcgcc tcggcctcac gatgttcgtc acgaacgaga cgcgctggac
     4321 caaccagaac ggcgagctgg tctgcgtgaa gacgcaggtc agcatccgct actagatcgc
     4381 cgaaggggag gcatttccga tggcctacaa cgtgtacttc gaggacgtga acgtcggcga
     4441 cgagctgccc gggttcaagc gtcgcacgga cctgatgaac tggaaccgct acgcggcgat
     4501 taacgacgag ttcatcccga tccacatgga tgacgcggcc ggccgacagg gccagaacga
     4561 gcagggcgcc ttcggcatgg gcaacctgcg gaccgcgtac atccacaaca tgctgcgcaa
     4621 ctgggccggc gacgaagcgg agatccgcga ggtcggcatc cagttccgcg cgatcaacca
     4681 gaagaatgac cagctgacga cgaccggcaa gatcgtcgag aagtacgtcg atggaaacga
     4741 ccacctcgtg cgcatcgaga ccaacgtcgt gaatcaggag ggcgctggta cggccccggg
     4801 gcacgcgatc atctgcctgc cgtctcgagg ctagtccgaa ccttctcgac agcgcagcac
     4861 cgaagcgggg agggccagac cctccccgct tcgcgtcacc gtcacttgta aattacagac
     4921 ccttcaacaa ttccggcgac tcgcgcaaca cctcggacgt accttcgaat cgaaggaggt
     4981 gcgtatgcga acacgagtcg cgttgcttgg aggcgtagcc gcggcggcat tgctcgtcgc
     5041 gggcgtggtc ggagtggctg cgcagacagg cgacagcggt tcagccaacc cggtgagcac
     5101 gttcatggac cggctggcgt ccaaccttgg cattgggaag gaccagttgc aggcggcggt
     5161 cgacaagacg cgcgggcaga tgctcgacga cgccgtgcag cagggacggt tgacccagga
     5221 ccaggccgac cagctgaagc agcagagcgc tggcaagggc ctcggggagg gcctgggacg
     5281 gttccactct ggggccggcg ccgagggtaa ccacgatggc cagccattcg gccggggcgg
     5341 ccccggcatg cgaggactgg cgggcgccgg gctcgatgct gtggccagcg caatcggcat
     5401 cgacaaagcg accttgatgc aggagctggc ctccggcaag tcgctggctc aggtggcgca
     5461 ggcgcacggc gtaagccgtg accagctgaa gcagaagctg acggacgcct tcggctcgaa
     5521 gctcgacgag ctgctggacc agacgttcca gttcggagct cacggcggca accccagcgc
     5581 gacgccgacg ccgtcgtcct gacgaacccg tctcttcgag acgactgaga gggaggggcc
     5641 cgcggagggc ccctctccta ttcgcgctcc atcagccgcg accgcgctgc gttcacgacg
     5701 cggatcgcgc gcacaacctc gcggacgtca tgaacacgca cgacgtggac cccggaccag
     5761 gcggcgagca cgttgaacgc gatggttgct tcgatcaggc cctcgacgtc ctgccgatca
     5821 cccgcgccca gtaactcggc gaggaaccct ttccgcgagg cggaaacgag tacgggtcgt
     5881 cccaccgagg tcatgcgagg aagggcgttc agcaaccgga ggttgtccgc ggcggacttg
     5941 ccaaagccga acccggggtc gagccagaca ccggcgacgc cggcggactc gagggcgttc
     6001 gcctggcggg tcaggaagac ctccacgtcg tgagcgatgt tgtcgtaggt ctggtcggcc
     6061 tcccggtggt gcttcggcgc gccgcgcatg tgcatcgcca cgacggggag ccggcagcgg
     6121 acggccacgt cgaccatcgc tggattggtg aagccggtca cgtcgttcag gagatggacg
     6181 cccgcgcggg ccgcctcgtt cgcgacggca ggcgtccagg tatcgacaga cacaggaata
     6241 ccggcagcgg cgatcgcctc gatggccggg cagacgcgat ctacctcctc ctgcgcggtc
     6301 aggtcacggc cgcccgtccg cgtggagtgc gccccgatat cgatgatctc ggcacccgca
     6361 tcccggagct cgagcgcgcg ctcgagcgcc tggccgggcc ggacgatcga ctcgctgatc
     6421 ggcgagtcgt aggacacgtt gagaatgccc atgaccaccg tacggttggt gcagtcgatc
     6481 gtatggggac cgagctgcag cagcatcggt tagcccgccc ccgccgcgaa ggcatcgcgc
     6541 aacgcggacg aggtggccgc gtcgtgcggg aggtcgagcg tgagtagttc ggctccgagc
     6601 cgccggtaga gcgcaccgag ttcgctgtca ccgagcaccg ccaggtgccg cctgaccgtg
     6661 tcgacgtcgc cccgtgcgac gggcccagtc aacgcgtcgc gcaggtcgtg ctcggcgacg
     6721 ttctgcgcgg cgccaaggag cagcggaacg agcgacgtcc gtgcggaggc agcggatagg
     6781 ccggcaagcg tccatgcctc gcgagcggca gccatgagag ccacgacgtc gttgctggcg
     6841 aagaccgcgg cggcatgata cgcggcgcgg tcgactccct cgagacggac gacactcgcc
     6901 ccaaggcgcc cgaccagctc ttccaggacg cgaccgagcg gattcgatcc ctcgacgccg
     6961 caggtgatgc cccggaaacg ggcagcgccg ccctcgcggg agggaaacga ctggaggggg
     7021 tgcaggcagc ccacgagcgc accctggcgg actgcgccgg acaacggacc gaggtcgagg
     7081 gcgccgctgc agtggacgac ccactgtcct tggcgccagc gaatctcgga tgccagcgcg
     7141 gcgatcgcgg catccggcac cgtcaggaac acgaggtcac aggagtcggc gaccgcctgc
     7201 ggcgcgagca cggcgacgcc cgctcccagc gccgtcgcga gatgttccgc cgagcggacg
     7261 gtccggctcg acaccgcctt gacctgttcg ccagcggcga ccagcgcggt cgcgagcgct
     7321 gaaccgaggc ggccggcgcc gaagatcccc acgcgcacag gttcgtcggc acccaccttc
     7381 ggagcatagc ggctttgccc tactcggcgc ttcctgcgta cagtcgctga acgagtcgag
     7441 acggagcgta catggacgac gacgaggtcc tggttggcat cgatttcacc gccgatcggc
     7501 tgcgcatcgt gctcgccgac ctggagggcg agccggttca ccgcggtgaa tggccgctcc
     7561 ccgaactgaa cgacgcggcg gcgtggtcgt gggaagtcgg cggccgcatc gcgacgtcct
     7621 ttgc
//
LOCUS       DAORPG010000010         7594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_420_length_7594_cov_3.881682, whole genome shotgun sequence.
ACCESSION   DAORPG010000010 DAORPG010000000
VERSION     DAORPG010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 7594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7594
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_420_length_7594_cov_3.881682"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLX85_00505"
     CDS             <1..231
                     /locus_tag="PLX85_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61686.1"
                     /translation="RRRAVRSGEPRDRRPARLGVLMRVALRKAVQRLCGAWWWLWTGD
                     PSPSATRRRLEEDVRRFGGEVSWRGSSYFSDR"
     gene            228..668
                     /locus_tag="PLX85_00510"
     CDS             228..668
                     /locus_tag="PLX85_00510"
                     /inference="COORDINATES: protein motif:HMM:NF018101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HRC61687.1"
                     /translation="MSLRFKDVSELPAHLRGQVEPAASDGHGARRSKYGNERIEYKGQ
                     IFDSKLERDRYRQLEMLWEAGQVCWFHRQVRFDLEGGVIYRADFQVIWPEGLFQRVGY
                     EDAKGYLLPEAKNKLKQVRERYKIVIDLIMRRNGQLVSLPWPEL"
     gene            668..880
                     /locus_tag="PLX85_00515"
     CDS             668..880
                     /locus_tag="PLX85_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61688.1"
                     /translation="MYVITKWESIGQVYLRRIGDRGQVYTSEHWHESVRFREPRDAEV
                     VLTMLDSLKGEGFTVERLTARCERTA"
     gene            877..1758
                     /locus_tag="PLX85_00520"
     CDS             877..1758
                     /locus_tag="PLX85_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61689.1"
                     /translation="MSGQAWTYLNPRSLAPQPERLTEVRRTSRREKPKTRDERALDLF
                     FKGKTATGRSSGEGGGGSLTAEDVRHALGGLAPKPFRFGMSAFLGDAQSLRLLEGFLI
                     EELRWKAEAGEWLRKREDVERIERMASLMLFEVISARSRHDTFAHQERLPEGSAAVLC
                     PKCNGRGAFGDSRAVEKWRRYRRMVRRARLRNPFPKPEDQDPRSAWTKRRTLHDALIA
                     KSRVEEARAAAQLCEVCSGTGRFIFTETHRARAVAVSRRTWYRDWAGRYTELIEIPRR
                     WEAIAVAHVRRKLADRT"
     gene            1851..1926
                     /locus_tag="PLX85_00525"
     tRNA            1851..1926
                     /locus_tag="PLX85_00525"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1884..1886,aa:Met,seq:cat)
     gene            2016..2288
                     /locus_tag="PLX85_00530"
     CDS             2016..2288
                     /locus_tag="PLX85_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61690.1"
                     /translation="MTLEQLAALVAGIGAPVRTQPAVAPGQTEVARVVTEADAEILAQ
                     LHNAGPALIACAQVLARINVRRLAGKHTTVVADRKAMEIALRTFAA"
     gene            2285..3109
                     /locus_tag="PLX85_00535"
     CDS             2285..3109
                     /locus_tag="PLX85_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61691.1"
                     /translation="MKYLRQNTATPIRFGRFVDSTDAVTPEVALTITPAMRKLSKAGA
                     AFAATSDGSNAVHDTEGFYSCTLTATDLNMPGSLELSVVVSGACPYQDRFAVLTAQAY
                     DAMFIHPLIAQAIHYGQLQGGGAAAATLATSASSVDDYYNGRLAYVYDGTGAGQVGYI
                     QDYVGSSRGCVMAENWLTTVDATSKVIILPWSKLSTLAEMSAAYGAQQLSGALHAYNA
                     RPTRDQALLYVERFLAGLVQATSTTDNVKDVNGTSNLATITYSPDNTSPTSRLRTA"
     gene            3106..3282
                     /locus_tag="PLX85_00540"
     CDS             3106..3282
                     /locus_tag="PLX85_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61692.1"
                     /translation="MSAIGLRRLGLGSSGAKRVRRVGLGSIGTPPPAADTSLVTYVRT
                     EYFSKRPPQAKARP"
     gene            3284..4648
                     /locus_tag="PLX85_00545"
     CDS             3284..4648
                     /locus_tag="PLX85_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRC61693.1"
                     /translation="MFLVRGNPVTPGAPGTPSVAVATLNGSTLRVTPSGTAAAGWKSW
                     EVQYSATSSSGPWTTLESARSAIAFPLDVTGLTAGVTYWFRVKGTDNVGQVSAYSSAV
                     SGATTSKSSTPGFYIRPDAFGYYTKDAQRLATYDKIIPYPNIRGAEIPICWGRIEPVQ
                     GQYNWASVEADINRITQNRTNGKKVILALAFQNYGITSIPSTPQSQFGAVIVPDYIID
                     AGWCALRTGNGLMPKLDISGCMDRLIAWVQACAAQYDNDPYVEMVMIGETSSAYSGMS
                     SSGYNTQWQRMPAVLAAAFPNTWAGVAHNGLASQAGSKTMSDLMVSNGNVHGTEDIIG
                     FYDNEPPAGTWEGWGFWAVMGAGVQDGYDFGSTDSRMAIPIRAENQVIRNPNLSLAQI
                     NAVADNTWKSTHTVFVLYFGADGNSYTPSNYGGSTPVPQQSGDNVLAYLANTANGVAR
                     TVLP"
     gene            4648..5349
                     /locus_tag="PLX85_00550"
     CDS             4648..5349
                     /locus_tag="PLX85_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61694.1"
                     /translation="MPWALRNVASPSFTSDARSGSGGLDVTLPSYQVNDILVMGASAR
                     PSNGFTVGSPPTGWSSLRDGTANETKLFVKIAAASEATPNVVCSETGAFGACIAAFSG
                     GPASLAGILHASALADSGGSLVEDIATAALTITIAQTLELYFGAASGNEWVAGTQPNG
                     ASVLCAANFAGAFNNLWLGFAYRVQGAAASLAASKFDTTSVNTVRTNSIVASLVPGQT
                     SAAAAQAYYRSLLVR"
     gene            5355..5546
                     /locus_tag="PLX85_00555"
     CDS             5355..5546
                     /locus_tag="PLX85_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61695.1"
                     /translation="MRVKLLQDVQENGQIKTTVRLKRKQVIGWFAGTELEMSDATARK
                     YIDAGLAEAAPAAAEEIQP"
     gene            5543..5977
                     /locus_tag="PLX85_00560"
     CDS             5543..5977
                     /locus_tag="PLX85_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61696.1"
                     /translation="MSASNAMENKLLSLYFENANAANVGDATGLRGSTTAGSTYCGLT
                     TADPGEAGSQTTSETAYSNYARQAVARSTAGWTVASGVADNDAAITFPTCGVTGSTIT
                     NTTLGSAVSGAGDLDFSGTASLVISQNITPSFGIGQLDISSD"
     gene            5981..6436
                     /locus_tag="PLX85_00565"
     CDS             5981..6436
                     /locus_tag="PLX85_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61697.1"
                     /translation="MSEILQQRHVEVAAQGEIVTMAIGNVRWSLEFDLALLIASGLRH
                     FGRIAKLCAGDQHNFFAMSGVLVDLNAPKPRRKRWGEALPERLRKHDVTVNHRGQLVT
                     LRIGRHTVSFPYAEAQKVAKWLRLRGKQARNAANERAHWSRIAPMEAFA"
     gene            6433..7446
                     /locus_tag="PLX85_00570"
     CDS             6433..7446
                     /locus_tag="PLX85_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61698.1"
                     /translation="MTQGRGMRSRGFALILGWLLAGTAIAAGWSLPDKGEGDSNIQSI
                     MFQEYLDALQEGVQGLNYVVSGGAITGGADMTPAIADVVVVSNGTVRAVTGADVTITT
                     ADATNPRLDLVVVNTSGSLAVRAGTAAANPKPPARTANDVLLWVVYVPANDTAIGTSQ
                     AVDLRVFRDDIGREYLLRQDSSYTLTSQTAAQQLFNASTNGRITLETGAYEFDIIVAL
                     TGMSATSGNATFDLAGTATLGTILWHGIGRDAASDATTGTYAGSYSTDATLTAAPLVT
                     AGTGTTAFFKIEGTFEVTAAGTLQPRILLQTASAAVVTAGSYIKIRKLSGSTSFTTFG
                     RWD"
BASE COUNT         1266 a         2633 c         2563 g         1132 t
ORIGIN      
        1 cgtcgccgcg ctgttcgcag cggcgagcct cgggatcgtc gccctgctcg cctgggcgtt
       61 ctcatgaggg tcgcgctgcg caaggccgtg cagcgcctct gcggcgcctg gtggtggctc
      121 tggacgggcg atccttcgcc atcggccacg cgccgccgcc tcgaggagga cgtgcgccgc
      181 ttcggcggcg aggtgtcctg gcgcggctcc agctacttca gcgaccgatg agcctgcggt
      241 tcaaagacgt ctcggaactc ccggcgcacc tgcgcggcca ggtggagccc gccgcgagcg
      301 atgggcacgg ggcgcgccga tcgaagtatg gcaatgagcg gatcgagtac aagggccaga
      361 tcttcgattc gaagctcgag cgcgaccgct accgccagct cgagatgctg tgggaggccg
      421 gccaggtctg ctggtttcac cgccaggtgc gcttcgacct cgagggcggc gtgatctacc
      481 gggcggactt ccaggtgatt tggcccgagg gactcttcca gcgcgtcggg tacgaggacg
      541 cgaagggtta cctgctgccc gaggcgaaga acaagctgaa gcaggtgcgg gagcgctaca
      601 agatcgtgat cgacctcatc atgaggcgga acggccagct cgtctccctg ccctggccgg
      661 agctctgatg tacgtcatca cgaagtggga gtcgatcggc caggtctacc tgcgccgcat
      721 cggggatcgc ggccaggtgt acacgagcga gcactggcac gagtcggtgc ggttccgcga
      781 gccgcgggac gccgaggtcg tgctgacgat gctcgacagc ctcaagggcg agggattcac
      841 cgtcgagcgc ctgacggcgc gctgcgagcg gaccgcatga gcggccaggc gtggacctac
      901 ctcaacccgc gcagtctcgc gccccagccg gagcggttga cggaggtccg taggacgagc
      961 cgtcgagaga agcccaagac gcgcgatgag cgggcgctcg acctgttctt caagggcaag
     1021 accgcgaccg gtcgctcatc gggggagggc ggtggcggct ccctgacggc cgaagacgtg
     1081 cgccacgcgc tgggcggcct tgctccgaag cccttccggt tcggcatgtc ggcgttcctc
     1141 ggcgacgcgc agagcctgcg cctgctcgag ggcttcctca tcgaggaact gcgctggaag
     1201 gcggaggccg gcgaatggct gcgcaagcgc gaggacgtcg agcggatcga gcgcatggcc
     1261 tccctgatgc tgttcgaagt gatcagcgcc cgctctcggc atgacacctt cgcgcaccag
     1321 gagcggctcc cggagggctc cgctgccgtc ctctgcccga agtgcaacgg tcggggcgcc
     1381 ttcggcgatt cgcgggcggt ggagaagtgg cggcgttacc ggcgtatggt gcgcagggcg
     1441 cgcctccgta atccattccc gaagccggag gatcaggacc cgcggtctgc ctggacgaag
     1501 cgcaggaccc tgcacgacgc cctgatcgcg aagtcacggg tcgaggaggc ccgcgccgcc
     1561 gcccagctct gcgaggtctg cagcggcacc gggcggttca tcttcacgga gacgcaccgc
     1621 gcgcgcgccg tcgcggtgtc ccgccggacc tggtatcgcg actgggccgg tcgatatacc
     1681 gagctgatcg agatcccgcg gcgctgggag gccatcgccg ttgcccatgt gcggcgcaag
     1741 ctcgccgatc gcacttgacg atggcacaga tctaccccac catctaggcg tgatgggcga
     1801 ttccgccccg cgttcagaga aaccccgccg cgagcggggt ttttcatttc tgcggctgta
     1861 gatcaccagc agatcgcctg gctcataacc aggagacagc gcgtgcaatt cgcgccgccg
     1921 caaccagttt gatacccccc agcccgcacg ttgcttcggc acgcgggcgc ggcccattca
     1981 cgggccgctc tcttccagcc agccccggag acgcgatgac gctcgagcaa ctggccgcgc
     2041 tcgtcgcggg tatcggggcg cccgtgcgca cccagccggc ggtggccccc gggcagacgg
     2101 aagtcgcgcg cgtcgtcacc gaggcggacg ccgagatcct cgcccagctc cacaacgccg
     2161 ggcccgcgct catcgcctgt gcccaggtgc tcgcgcggat caacgtgcgg cgcctcgcgg
     2221 gcaagcacac gacggtcgtc gctgatcgta aggccatgga gatcgccctg aggaccttcg
     2281 ccgcgtgaag tacctgcgcc agaacaccgc gacgccgatc cgcttcggcc gatttgtcga
     2341 cagcacggac gcggttacac cggaggttgc gctgacgatc acgccggcga tgcgcaagct
     2401 ctcgaaggcc ggcgcggcat tcgccgcaac gtccgacggc agcaatgcgg tgcatgacac
     2461 cgagggtttc tactcgtgca cgctcacagc gaccgatctg aacatgcccg gatcgctcga
     2521 gctgtcggtc gtcgtctcgg gcgcctgccc gtaccaggat cgattcgcgg tcctgacagc
     2581 tcaggcctac gacgcgatgt tcatccatcc gctgatcgcg caggcgatcc actacgggca
     2641 gttgcagggc ggtggtgcgg ccgctgcgac cctcgcgacc tcggcgagct cggtcgacga
     2701 ctactacaac ggccgcctgg cctacgtgta cgacggcacg ggcgcgggcc aggtcggcta
     2761 catccaggac tacgtcggca gctcccgcgg ctgcgtgatg gccgagaact ggctgacgac
     2821 cgtcgatgcg acctcgaagg tgatcatcct gccctggtcg aagctctcga ccctggccga
     2881 gatgtcggcg gcctatgggg cccagcagct cagcggcgcg ctgcacgcct acaacgcgcg
     2941 cccgacccgc gaccaggcgc tgctctacgt cgagcgcttc ctcgccggcc tcgtgcaggc
     3001 gaccagcacg acggacaacg tgaaggacgt gaacggcacg agcaacctcg cgaccatcac
     3061 gtactcgccc gacaacacct cgccgacaag ccggctgcgc acggcatgag tgcgatcgga
     3121 ttgaggcggc tcgggctcgg gagctcgggc gcgaagcgcg tgcggcgcgt cggcctgggc
     3181 tccatcggca cgccgccgcc ggcggccgac acgagcctcg tgacgtacgt ccgcacggag
     3241 tacttctcga agcgtccgcc ccaggccaag gcgaggccct gacgtgttcc tggtgcgtgg
     3301 caacccggtg acgccaggcg caccggggac gccctccgtg gccgtcgcga ccctgaacgg
     3361 ctccacgctg cgcgtgacgc cctcgggcac cgccgccgcc gggtggaagt cctgggaggt
     3421 ccagtactcg gcgacgagct cctcgggccc ctggacgacg ctcgagagtg cacgcagcgc
     3481 gatcgccttc ccgctggacg tgacgggcct cacggccggc gtgacctact ggttccgggt
     3541 caagggcacg gacaacgtag gtcaggtctc ggcctactcg agcgcggtca gcggtgcgac
     3601 gacgtcgaag tcctcgacgc cgggcttcta catccgcccc gatgcgttcg gctactacac
     3661 gaaggacgct cagcggctcg cgacgtacga caagatcatc ccgtacccga acatccgcgg
     3721 ggccgagatc ccgatctgct ggggtcgcat cgagccggtc cagggccagt acaactgggc
     3781 gagcgtcgaa gccgacatca accggatcac gcagaaccgg acgaacggta agaaggtgat
     3841 cctcgcgctc gccttccaga actacggcat cacgagcatc ccctcgacgc cgcagagcca
     3901 gttcggggcc gtgatcgtcc ccgactacat catcgacgcc ggctggtgtg cgctgcgcac
     3961 cggcaacggc ctgatgccga agctcgacat ctcgggctgc atggatcgcc tgatcgcctg
     4021 ggtacaggcc tgcgccgccc agtacgacaa cgatccgtac gtcgagatgg tgatgatcgg
     4081 ggagacgagc tcggcctact cgggcatgag ctcgagcggc tacaacaccc agtggcagcg
     4141 gatgccggcg gtgctcgccg cggccttccc gaacacctgg gcgggcgtcg cccacaacgg
     4201 gctcgcctcc caggccggca gcaagacgat gtcggacctg atggtctcga acggcaacgt
     4261 tcacgggacc gaggacatca tcggcttcta cgacaacgag ccacccgccg gcacctggga
     4321 gggctggggg ttctgggccg tcatgggcgc gggcgtccag gacggctacg acttcggctc
     4381 gacggactcg cgcatggcga tcccgatccg ggccgagaac caggtcatcc gcaacccgaa
     4441 cctctcgctc gcgcagatca acgcggtcgc ggacaacacg tggaagtcga cgcacacggt
     4501 gttcgtcctg tacttcgggg cggacggcaa cagctacacc ccgagcaact acggcggctc
     4561 gacaccggtg ccgcagcagt cgggtgacaa cgtgctcgcc tatctcgcga acacggccaa
     4621 cggcgtggcg cggacggttc taccgtagtg ccctgggcgc ttcgcaacgt cgcctcgccg
     4681 tccttcacga gcgatgcgcg ctcgggctcg ggtggcctgg acgtcacgct gccgagctac
     4741 caggtcaacg acatcctggt gatgggcgcc tcggctcgcc cctcgaacgg gttcacggtg
     4801 ggctcgccgc cgaccggctg gtcgtcgctg cgggatggca ccgcgaacga gacgaagctc
     4861 tttgtgaaga tcgccgcggc gagcgaggcc acgccgaacg tcgtctgcag cgagacgggc
     4921 gccttcgggg cctgcatcgc ggcgttctcg ggcggccccg cgagcctcgc gggcatcttg
     4981 catgcgagcg cgctggcgga ctcgggcgga tcgctggtcg aggacatcgc cacggcggcc
     5041 cttacgatca cgatcgcgca gacgctggag ctgtacttcg gcgctgcctc cggcaacgag
     5101 tgggtcgcgg gcacgcagcc gaacggcgcg agcgtccttt gcgcggccaa cttcgccggc
     5161 gccttcaaca acctgtggct cggcttcgcc taccgggtcc agggagcggc cgcgagtctc
     5221 gccgcctcca agttcgacac gacgagcgtc aacacggtgc gcaccaacag catcgtcgcc
     5281 tcgctcgtcc ccggccagac cagtgcggcg gccgcccagg cctactaccg aagccttctc
     5341 gtgaggtaac cgagatgcga gtgaagctcc tccaggacgt tcaggagaac ggccagatca
     5401 agaccaccgt gcgcctcaag cgcaagcagg tcatcggctg gttcgccggt accgaactcg
     5461 agatgtcgga tgcgacagcc cggaagtaca tcgacgccgg cctcgccgag gccgcaccgg
     5521 ccgccgctga ggagatccag ccgtgagcgc atccaatgcg atggagaaca agctgctgag
     5581 tctctacttc gagaacgcca acgcggcgaa cgtcggagac gcgaccggcc tgcgcggctc
     5641 gaccacggcg ggcagcacgt actgcggcct gactaccgcc gatccgggag aggcgggcag
     5701 ccagactacc agcgagacgg cgtactcgaa ctacgcccgc caggccgtgg cacgcagcac
     5761 cgccggctgg acggtggcct ccggcgttgc ggacaacgat gcggcgatca cgttcccgac
     5821 gtgcggcgtc acggggtcga caatcaccaa tacgacgctc ggctcggcgg tgtcgggtgc
     5881 gggcgatctc gacttcagcg gtaccgcatc gctcgtgatc agccagaaca tcaccccgag
     5941 cttcggcatc ggccagctcg acatctcgag cgactgaggc atgagcgaga tcctccaaca
     6001 gcgccatgtg gaagtggcgg cgcagggcga gatcgtgacc atggccatcg gcaatgtgcg
     6061 ctggtcgctc gagttcgacc tcgcgctcct gattgcttcc ggactgcgcc acttcggcag
     6121 gatcgcgaag ctctgcgcgg gagatcagca caacttcttc gcgatgagcg gtgtgctcgt
     6181 tgacctcaat gcgccgaagc cccgccgcaa gcgctggggc gaagcgctgc cggagcgtct
     6241 gcgcaagcac gacgtgaccg tgaaccaccg cggtcagctc gtcacgctgc gcatcgggcg
     6301 acacacggtg agcttcccgt acgccgaggc gcagaaggtc gcgaagtggc tccgcctgcg
     6361 cggcaagcag gcgcgcaacg ccgccaacga gcgcgctcac tggtcgcgga tcgcgccgat
     6421 ggaggcgttc gcatgacgca ggggcggggc atgcgctcga ggggcttcgc gctgatcctc
     6481 ggctggttgc tcgccggcac cgcgattgcc gccggctgga gcttgcccga caagggcgag
     6541 ggcgacagca acatccagtc gatcatgttc caggagtacc tggacgcgct tcaggaaggc
     6601 gttcagggac tgaactacgt cgtctcgggc ggcgccatca ctggcggcgc ggacatgacg
     6661 ccggccatcg cggatgtcgt ggtggtcagc aatggcacgg tgcgcgcggt caccggcgcg
     6721 gacgtcacga tcaccacggc ggatgcgacg aacccccggc tcgacctggt cgtcgtcaat
     6781 acgtcgggct cgctcgccgt ccgcgcgggc accgcagcgg cgaacccgaa accaccggcg
     6841 cgcaccgcga atgacgtcct gctgtgggtg gtgtacgtcc cggcgaacga caccgcgatc
     6901 gggacctcgc aggccgtcga cctgcgcgtg ttccgcgacg acatcggccg cgagtacctg
     6961 ctgcgccagg actcgtccta cacgctgacc agccagaccg cggcgcagca gctcttcaac
     7021 gcctcgacga atggccgcat cacgctcgag acgggcgcct acgagttcga catcatcgtc
     7081 gcgctcacgg gcatgtcggc gaccagcggc aacgcgacct tcgacctcgc cggcaccgcc
     7141 accctcggaa caatcctctg gcacggtatt ggccgcgacg ccgcgtcgga tgcgacgacc
     7201 ggcacgtacg ctggtagcta ctcgacggac gcgacgctca ctgccgcacc gctcgtgacc
     7261 gcgggaacgg gtacgaccgc gttcttcaag atcgagggca ccttcgaggt gaccgcggcc
     7321 ggcacgctgc agccgcgcat cctgctgcag accgcctcag ccgcggtcgt cacggccggc
     7381 agctacatca agatccgcaa gctcagcggc tcgacttcgt tcaccacctt cggacgctgg
     7441 gactgacatg cgcaaactcc tccccttcct gctcggactg atcccgactc tcgcgctggg
     7501 cgcggtcaac tcggttgcga cgcccggcga ctgggcgctc tatcgtggcc cctcgatcgt
     7561 ctcccgccat gccagcgagg cggcatgcat cacg
//
LOCUS       DAORPG010000011         7556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_424_length_7556_cov_3.700840, whole genome shotgun sequence.
ACCESSION   DAORPG010000011 DAORPG010000000
VERSION     DAORPG010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 7556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7556
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_424_length_7556_cov_3.700840"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /gene="dnaX"
                     /locus_tag="PLX85_00575"
     CDS             complement(<1..1214)
                     /gene="dnaX"
                     /locus_tag="PLX85_00575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRC61699.1"
                     /translation="MSQEVLYRKWRPQRFADVVGQQPITTTLRHAVASGSPAHAYLFT
                     GPRGTGKTSTGRILAKAVNCLEPVEGEPCNTCTSCEAFQSGRALDLIELDAASNRGID
                     EVRALREAAGYAPNSARYKVYLIDEVHMLTDAAFNALLKTLEEPPPHIIFILATTEAH
                     RIPATITSRCQRFDFRRHTLANTVEWLQVIADGEGMTVAPGCLELIARQATGSMRDAV
                     NLLDQLVAYHGRTLDLDAVQRGLGLVVDHRTTELAGAAVKRDLSAGLAVLAAARDDGL
                     DIRAFAREVVATLRSLLLIRAGATDQLSLSESQTAELREIANEVGSADIVAALRAFGD
                     LDFAGDPYDSLPAEIAFASLAVGLTANTGQPPVAAAPTQPAEGRATRSAPAERRSSSA
                     APRQGQRARTPQP"
     gene            complement(1295..1795)
                     /gene="ybeY"
                     /locus_tag="PLX85_00580"
     CDS             complement(1295..1795)
                     /gene="ybeY"
                     /locus_tag="PLX85_00580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRC61700.1"
                     /translation="MPIDLLLEFDDSLEADDRAIAEGADLPALTRVLEDEALAGDIEL
                     TVLLGGPALLRALNHEHRGLDEPTDVLSFPAAEGEEFPVAPGEPRYLGDIAVSVPTVR
                     ENAAAASLAADLELRHVVVHGMLHLAGYDHETPEDDAVMRAREETVLGAGIHAGRGDE
                     AHSDHD"
     gene            complement(1807..3273)
                     /locus_tag="PLX85_00585"
     CDS             complement(1807..3273)
                     /locus_tag="PLX85_00585"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRC61701.1"
                     /translation="MDSLHQLHHIAPAATLLVGAAVVLIADLASNRRSDVQWLALLTI
                     VVATLMACWQVLAGLPGESALGGAVIVDVFGLYFAFLLLAVAAAVVIVSSDGLQRVEQ
                     RGEFYALLLTSTASMVLLAQAQDLILIFVALETTSIVQFVLAGIARDDRSSEAGLKYL
                     LTGAISAAVLLYGFAFLFGLAGTTSLTGIAAFVGAGAEETRLAVILAFVLVAAGLGFK
                     MAIVPFQAWVPDVYQGSPTPVTTFLSVASKAGGFAIVLRIFYTGLGGGDTFVSEDWAK
                     MFAVFAAVSMTFGNVAAIQQTDAKRLLGYSSIAQAGNIAVGLAAVAGGSTLGASAVLF
                     FLGTYAATNLGVFISVIAVSKSIGSDDIRDYAGLLRRSPGVALVLSLCLLSLTGIPPT
                     AGFLAKVYVFNSAVRTGQDWLVWLIVVAVLNTAISAYYYLRWARIMILDEPRDTLTFR
                     PTLTTSGLLMLAGAGVLFFGLVPTPLIEAARRAAETLV"
     gene            complement(3276..4748)
                     /locus_tag="PLX85_00590"
     CDS             complement(3276..4748)
                     /locus_tag="PLX85_00590"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRC61702.1"
                     /translation="MLTAFLLLPIAGALVIALLPRDRAADARWVALIASGLAFALSIL
                     IFVRFDPDQAGYQMVDRFDWIRAGDAGFSVQYVLGVDGLSTPMVLLTGLLSVAAVLVS
                     FNVTLRTKEYFAWLLVLETAVAGVFLSLDLIQFFLFWELELIPMYLLISIWGSGRKEY
                     SAMKFLLYTIAGSALMLVGFLVLGFTAGTFDIAKLTASPPTEAAIPLAAIFWFIMAAF
                     LVKLPVFPVHTWLPDAHTDAPTAVSVMLAGVLLKMGGYGILRIVLPILPQQAHDFRVL
                     LAGIAGFSVIYGALITLRQRDLKRLVAYSSVSHMGYVLLGIAALGPVGLSGAALQMFT
                     HGTITGLLFIMVGLVYDRAHTRQISELSGLMHRMPLIGVVMVVAGLAALGLPGLSGFV
                     AEVTVFLGSLGPHPAATIFAVFGVVLAAGYILWTVQRVFTGPPNERWADLTDANQWWE
                     RTAMGGMLVAIFVVGIYPRLLTDVIQSGIDPIAAIVQRGG"
     gene            complement(4749..6671)
                     /gene="nuoL"
                     /locus_tag="PLX85_00595"
     CDS             complement(4749..6671)
                     /gene="nuoL"
                     /locus_tag="PLX85_00595"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRC61703.1"
                     /translation="MSDIPEGAAWAIYFVPLSAAVLIAALLRGRPREAGRLMIVAVGV
                     SWLLALWALATVRDHDGEAVGFAAHSWVQLFNLDVAFGIRLDGLTGVMLVVVTSVALL
                     VQVYSTEYMRGDGGYSRYYAYMGLFTSSMLGLVLASSLLQLFIYWELVGLSSYLLIGF
                     WFQRPSAAAAAKKAFIVTRFGDFGFLLGVLLIWSKTGDFDIATINAHAEELSAAVLAG
                     FTLGLFAGAVGKSAQFPLHIWLPDAMEGPTPVSALIHAATMVAAGVYLIARFFPTFEA
                     APSEVRTLIAYIGAVTALLAATMGIVATDIKRVLAYSTISQLGYMVMALGLGGYVAAI
                     FHLFTHAFFKALLFLGSGSVNHATGTFDMRKMGGLRSSMPVTWLTFLIGSLSLSGVFP
                     LAGFWSKDEVLLDAWEHNRLLFAVGVIVAFMTAFYMFRAIFMTFFGEYRGGEASEHGS
                     DAAHHSGPHESPLPMWAPLAILAVPAVLIGFVNVTGGFGHLIEGALPEALREGHPDLD
                     RVVLVSSTLAALFGIGAAAAIYFARTPAPEMLRSRLGPLPTVVENKYYMDVIAEDIIV
                     RRVLYGGLARAAELFDTYVVDMAVNTAGRATRLASEFSRRTTFGQQQAYSSLLLAGVV
                     AAVVVLFVLSGNVLER"
     gene            complement(6671..6859)
                     /locus_tag="PLX85_00600"
     CDS             complement(6671..6859)
                     /locus_tag="PLX85_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61704.1"
                     /translation="MWVKFMEVPDGYAAVIWKELFNTEALSVRVVPPLELGSMRDARE
                     IWVPDGKTHVAREVLNKI"
     gene            complement(6876..7202)
                     /gene="nuoK"
                     /locus_tag="PLX85_00605"
     CDS             complement(6876..7202)
                     /gene="nuoK"
                     /locus_tag="PLX85_00605"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015149227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRC61705.1"
                     /translation="MSVTLAHYLAVSGVVFGIGIAVALSKRNAIAVLMGVELMLNAVN
                     LTFLAFSRFSAAFSENSAPIAGHVFVVFVLTVAAAEAAVALAMAVLVYRRRETVDLDR
                     INLMRW"
     gene            complement(7199..>7556)
                     /locus_tag="PLX85_00610"
     CDS             complement(7199..>7556)
                     /locus_tag="PLX85_00610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRC61706.1"
                     /translation="DFIAVAQVLIYAGAISVLLVFAVMLTPLASRDNANSLFVLPGVL
                     IGAAFAGIVGFVAIDVDWPELTGKAVLGRDFTTTVQGIGSALLDPYVLAFEIASVLLL
                     SALVGAIVLVREDEPA"
BASE COUNT         1464 a         2523 c         2528 g         1041 t
ORIGIN      
        1 ggctgcggtg tgcgcgcccg ctgcccctgc cgcggcgctg ccgaggacga tcgtcgctcc
       61 gctggagcag aacgggtagc cctcccctcg gccggctgtg tcggcgcggc ggccacgggc
      121 ggctggcccg tattcgccgt caatccgacc gccagcgagg cgaacgcgat ctccgcgggg
      181 agcgaatcgt acggatcgcc cgcgaaatcg aggtcaccaa atgcccgcag agccgccacg
      241 atgtccgccg agccaacctc gttcgcgatc tcgcgaagct ccgcggtctg cgactcggag
      301 agcgaaagct ggtcggtcgc cccagcacga atgagcagca acgagcggag cgtcgccacg
      361 acctcgcgcg caaacgcccg gatatcgagg ccgtcatcgc gcgccgcggc gaggactgcg
      421 aggcccgcgc tgaggtctcg cttgaccgcg gcccctgcca actccgtggt gcgatggtcg
      481 acgacgaggc caagcccacg ctgtacggca tcgaggtcca gcgtgcgacc gtggtaggcc
      541 acgagctggt cgagcaggtt gactgcatcg cgcatcgagc cggtcgcctg ccgcgcaatc
      601 agttcgaggc agccgggggc gacggtcatt ccctcgccgt ccgcaatcac ctgcagccat
      661 tccaccgtat tggcgagcgt gtggcggcgg aagtcgaacc gctggcagcg cgacgtaatc
      721 gtcgcgggaa tccggtgcgc ctcggtcgtc gcgaggatga agatgatgtg cggcggaggc
      781 tcctcgagcg tcttgagcag cgcgttaaag gccgcgtctg tgagcatgtg cacctcgtcg
      841 atgagataca ccttgtagcg ggcgctgttt ggcgcgtagc ccgccgcttc tcgcaacgcg
      901 cggacctcgt cgatgcctcg gttcgaggcg gcatcgagtt cgatcaggtc gagcgcacgt
      961 ccgctctgga atgcctcgca ggaggtgcag gtattgcagg gctcgccctc gaccggctcg
     1021 agacagttca cagccttggc gaggatacgc cccgtgctcg tcttccccgt cccacggggg
     1081 cccgtgaaca gataggcgtg cgccggtgac ccgctcgcga cggcatgacg gagcgtcgtc
     1141 gtgatcggct gctggccgac gacgtcagcg aaccgctgtg gtcgccactt ccggtaaagg
     1201 acttcctggc tcacgtgcgt cctgctgcct cgggggagga atcgaggtca gtcgtgccag
     1261 tgtacacggt cacccgtgcg gcctcatccg aggttcagtc gtggtcgctg tgcgcctcat
     1321 cgccgcgtcc ggcgtggatc cccgctccga ggaccgtctc ttccctcgct cgcatgacgg
     1381 cgtcgtcctc cggcgtctca tggtcgtacc cggcgaggtg cagcatcccg tgcacgacca
     1441 catgccgcag ctcgagatcc gccgccaggc tcgcggcggc ggcgttctca cggacggtgg
     1501 gcaccgacac cgcgatatca ccgaggtagc gtggttcgcc gggcgcgacg gggaactcct
     1561 cgccctccgc tgccgggaac gagaggacat cggtaggttc gtccagccct cgatgctcat
     1621 ggttcagcgc ccgcagcaac gcagggcccc cgaggagcac cgtgagctcg atgtcgccgg
     1681 caagcgcctc gtcttcgaga acccgcgtca gcgccggaag gtccgcgcct tccgcgatcg
     1741 cgcggtcgtc agcctcgagc gaatcgtcga actcgagcag gaggtcgatg ggcacgtggg
     1801 ggcggcttag acgagggtct cggccgcgcg gcgtgccgct tcgatgagcg gggttggcac
     1861 gagcccgaag aacagtacac cggcgccggc gagcatcagt agcccgctcg tggtcagggt
     1921 cggccggaac gtcagcgtgt cgcgcggttc gtcgaggatc atgatgcggg cccagcgcaa
     1981 gtagtagtac gcggagatag ccgtgttcaa gaccgcaacg acaatcagcc acacgagcca
     2041 gtcctgaccg gtgcggaccg cgctattgaa cacgtacacc ttcgcaagga agcccgccgt
     2101 gggcgggatg ccggtgagcg agagcaggca caggctcagc acgagcgcca cgcccggcga
     2161 gcgcctgagc agtcccgcgt aatcgcggat gtcgtccgat ccgatcgact tcgagacggc
     2221 gatgacggag atgaagacgc cgaggttcgt agctgcgtag gtcccgagga agaacaggac
     2281 ggctgaggcg ccgagtgtgc tgccgccggc caccgccgcg agcccgaccg cgatattgcc
     2341 cgcctgcgcg atcgaggaat agccgagcaa ccgctttgcg tccgtctgct ggatcgcggc
     2401 cacgttcccg aacgtcatcg agacggcggc gaagacggcg aacatcttcg cccagtcctc
     2461 ggagacgaag gtgtcgccgc cgcccaaccc ggtgtagaag atgcgcagca cgatagcgaa
     2521 cccacccgcc ttcgaggcga ccgacaggaa cgtcgtcacc ggcgtcggcg aaccctggta
     2581 gacgtcaggc acccaggcct ggaacggcac gatcgccatc ttgaagccga gccccgccgc
     2641 tacgagaacg aacgccagga tgacagcgag gcgtgtttcc tcggctccgg cgccgacgaa
     2701 cgcggcgatc ccggtcaggg aagtcgtgcc ggcgagcccg aacaggaacg cgaagccgta
     2761 cagcaggacc gccgccgaga tcgcgcccgt gaggaggtac ttcaggcccg cctccgatga
     2821 gcggtcgtcg cgcgcgatgc cggccagcac gaactggacg atcgaggtcg tctcgagggc
     2881 cacaaagatg agaatcaagt cctgcgcctg tgcgagcagc accatcgacg ccgtggaggt
     2941 cagcagcagc gcgtagaact cgcctcgctg ttcgacccgc tgcaggccgt cgctcgagac
     3001 gatgacgaca gcggcggcga ccgcgagcag aaggaacgcg aagtacagac cgaagacatc
     3061 gacgatgacg gcgccgccca aggcagattc acccggcagg ccggccagca cctgccagca
     3121 cgccatcagc gtcgccacca cgatggtcag cagcgccagc cactggacgt cagagcgccg
     3181 gttcgatgcc aagtcggcga tcaacaccac ggccgcgccg acgaggagtg tcgcggccgg
     3241 cgcgatgtgg tggagttggt gcaggctatc caccgttagc cgcccctctg cacgatggcg
     3301 gcgatcgggt cgatgccgct ctggatcaca tcggtgagca ggcgcgggta gatgccgacg
     3361 acgaagatcg cgacgagcat gccgcccatc gcggtgcgct cccaccactg gttcgcgtcg
     3421 gtgaggtccg cccagcgttc attgggcggg cccgtaaaca cgcgctggac ggtccacagg
     3481 atgtagcccg ccgcaagcac caccccgaag accgcgaaga tcgtggccgc tggatgtggg
     3541 cccagcgacc cgaggaacac ggtcacctcg gcgacgaatc ccgagagccc gggcagcccg
     3601 agtgcggcga ggcccgcgac caccatcaca acgccgatca acggcatccg gtgcatcaga
     3661 ccgctcagct cggagatctg gcgcgtgtgc gcccggtcgt acacaaggcc gaccatgatg
     3721 aagagcagcc ccgtgatcgt gccgtgggtg aacatctgca gcgccgcccc ggacagcccg
     3781 accgggccga gcgcggcaat gccgagcaag acgtacccca tatgcgacac cgacgagtac
     3841 gcgacaaggc gcttcaggtc gcgctggcgc aacgtgatca gcgcgccgta gatgacgctg
     3901 aagcccgcga ttcccgccag cagcacacgg aagtcgtgcg cttgctgcgg aaggatcggc
     3961 agcacgatgc gcaagatgcc gtagccgccc atcttgagca gtacgccggc cagcatgacc
     4021 gacaccgcgg tgggcgcgtc ggtgtgcgcg tccgggagcc aagtgtgcac cgggaacacc
     4081 ggcagcttca cgaggaacgc ggccatgatg aaccagaaaa tggccgccag cgggatagca
     4141 gcctccgtcg gcggcgatgc cgtgagcttc gcgatgtcga acgtgccagc cgtgaagccc
     4201 agcacgagga accccacaag catcagcgcc gagccagcaa tggtgtacag caggaacttc
     4261 atcgcgctgt actctttgcg gcccgacccc cagatgctga tcagcagata catcgggatc
     4321 agctcgagct cccagaacag gaagaattgg atcaggtcga gcgacaggaa cacaccggcg
     4381 accgccgtct cgaggaccag cagccaggcg aagtactcct tggtccgcag cgtcacattg
     4441 aacgacacga gcaccgcggc cactgacagg agtccggtga gcagcaccat cggcgtcgac
     4501 aggccgtcga caccgaggac gtactgcacc gagaaaccgg catcgccggc acggatccag
     4561 tcgaagcggt cgaccatctg gtagccggcc tggtccgggt cgaagcggac gaagatcaga
     4621 atcgagagcg caaacgccag accggacgcg atgagcgcga cccatcgggc gtcggccgcg
     4681 cggtcgcgcg gcagcagggc gatcacgaga gcgccggcga tggggaggag caggaacgcc
     4741 gtcagcatct accgctccaa cacattcccg ctgaggacga agaggaccac gaccgccgct
     4801 acgacgcccg ccagcaacag cgagctgtac gcctgctgtt ggccgaaggt agtgcgccgc
     4861 gagaactcac tcgcgagccg cgtcgctcgg ccggctgtgt tcacggccat gtcgacgacg
     4921 taggtatcga acaactcggc cgcgcgcgcg agaccgccgt agaggacgcg ccgcacgatg
     4981 atgtcttcgg cgatcacgtc catgtagtac ttgttctcca cgaccgttgg cagcgggccg
     5041 aggcgggagc gcagcatctc gggagcgggg gtgcgcgcga agtagatcgc cgccgcggcg
     5101 ccgatgccga agagcgcggc gagcgtcgaa ctcacgagga cgacccgatc gaggtcgggg
     5161 tgcccctcgc gcagggcttc gggcagcgcg ccttcgatca ggtggccgaa gccgccggtg
     5221 acgttcacaa atccgatcag caccgccggg accgcgagga tcgccagggg cgcccacatc
     5281 gggaggggtg actcatgcgg tccgctgtgg tgcgcggcgt cactcccatg ctcggacgcc
     5341 tcgccgccgc ggtactcgcc gaagaacgtc atgaagatgg cgcggaacat gtagaacgcc
     5401 gtcatgaagg ccacgatgac gccaacggcg aaaaggagcc ggttgtgctc ccacgcgtcg
     5461 agcaacacct cgtccttgct ccagaagcca gcgagcggga acaccccgga cagcgacagc
     5521 gacccgatca ggaacgtcaa ccaggtgacc ggcatggaag agcgcaggcc gcccatcttc
     5581 cgcatgtcga aggtaccggt cgcgtggttc acggaacccg agccgaggaa cagcagcgcc
     5641 ttgaagaagg cgtgcgtgaa gaggtggaag atcgccgcca cgtacccgcc gagcccgagg
     5701 gccatcacca tgtacccgag ctgcgagatc gtggagtacg ccagcacgcg tttgatgtcc
     5761 gtcgcgacga tgcccatcgt cgccgccagt agcgccgtga cggcgccgat gtacgcgatc
     5821 agggtacgca cctcgctcgg cgccgcctcg aaggtcggga agaagcgcgc aatcaggtag
     5881 acaccggccg ccaccatcgt ggcggcgtgg atgagcgcgg acaccggcgt cgggccttcc
     5941 atcgcgtccg gcagccagat gtgcaatggg aactgcgccg acttgccgac agcgcccgcg
     6001 aataacccca gtgtgaagcc cgcgaggacc gcggccgaca gctcttcggc gtgcgcgttg
     6061 atcgtcgcga tgtcgaagtc gcccgtcttc gaccagatga gaagtacgcc gagcaggaag
     6121 ccgaagtcgc cgaatctcgt aacgataaac gccttcttcg cggcggcggc ggccgagggc
     6181 cgctggaacc agaacccgat caggaggtac gaggacaggc cgaccaattc ccagtagatg
     6241 aagagctgta gcaggctcga tgcgagcacc agcccgagca tcgaggaggt gaacagaccc
     6301 atatacgcgt agtagcggct gtagccgcca tccccgcgca tgtactcagt ggagtagacc
     6361 tgcacgagca gcgccacgct ggtgaccacc accagcatca cgccagtcag tccgtcgagg
     6421 cgaatgccaa acgccacgtc gagattgaat agctgcaccc agctgtgagc agcaaagccc
     6481 acagcctcgc cgtcgtggtc gcgcaccgtc gcgagcgccc agagggccag cagccaggac
     6541 accccgaccg cgacgatcat gaggcgcccg gcctcgcgtg gccgcccacg caacagtgcc
     6601 gcgatcagca ctgcggcgct gagcgggacg aagtagatgg cccaggctgc gccctcgggg
     6661 atgtcgctca tcagatcttg ttcaacactt cgcgcgcaac gtgcgtctta ccgtcgggga
     6721 cccagatctc ccgtgcgtcg cgcatcgagc caagttcgag cggcgggacg acgcggaccg
     6781 acagcgcttc ggtgttgaag agctccttcc agatgacggc cgcgtagccg tccggaacct
     6841 ccatgaactt cacccacatc ggcgcacgct ccgcatcacc agcgcatcag gttgatgcgg
     6901 tcgaggtcta ccgtctcgcg gcgacggtat acgagcaccg ccatggccag cgcgacggcc
     6961 gcctcggcag ccgcgaccgt gagcacaaat acgacgaaga cgtggcccgc gatcggcgcc
     7021 gaattctccg agaaggccgc gctgaagcgt gagaacgcga ggaacgtcag gttcaccgcg
     7081 ttgagcatga gctcgacgcc catgagcacg gcgatcgcat tgcgcttcga cagcgcaacg
     7141 gcaatgccga tcccgaagac gacgcccgat accgcgaggt agtgcgccag tgtcacgctc
     7201 atgcgggctc gtcctcccgt acgaggacaa tcgcgcccac gagcgcggag agcagcagga
     7261 ccgacgcgat ctcgaatgcg agcacgtatg ggtcgaggag cgccgagccg atgccctgca
     7321 ccgtcgtggt gaagtcgcgg ccgagcaccg ccttcccggt cagctcgggc cagtcgacat
     7381 cgatcgccac gaacccgacg atgcccgcga atgcggcgcc gatgagcacc cctggcagca
     7441 cgaatagcga gttcgcgttg tcgcgcgaag ccagcggtgt gagcatcacg gcgaagacca
     7501 atagcaccga gatggcgccg gcgtagatca gcacctgcgc taccgcgatg aagtca
//
LOCUS       DAORPG010000012         7420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_441_length_7420_cov_3.720299, whole genome shotgun sequence.
ACCESSION   DAORPG010000012 DAORPG010000000
VERSION     DAORPG010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 7420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7420
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_441_length_7420_cov_3.720299"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PLX85_00615"
     CDS             <1..820
                     /locus_tag="PLX85_00615"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRC61707.1"
                     /translation="GRLRAVAEAAGAETLVSQLSRDGSDEDLGADLVGADVLVCVGGD
                     GTVLHATGIASSAGVPVFGVRMGRLGFLAEATAGEAEDALTRIIGGEGRLERRVMLQA
                     QVGEAAPQHALNDVVIGRMHLGRTVTVGLRVDGVLLAEYRADAVMAATATGSTGYSLS
                     VGGPILHPTSDDLIVTAVAPHLSYPNALVLPGDASIEFEVARGYEAVLLVDGGHEQAV
                     TSGTFVRVTRSPREALFLRLGAERQFYANLARRLGWLRIDHVVGNDDPAEAGLS"
     gene            complement(813..1904)
                     /locus_tag="PLX85_00620"
     CDS             complement(813..1904)
                     /locus_tag="PLX85_00620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010986186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRC61708.1"
                     /translation="MQFGIFSVGDVTPDPLSGRAPSEAERIQSMVAIALKAEQVGLDV
                     FATGEHHNPPFVPSSPTTLLGYIAGRTKRIVLSTATTLITTNDPVKIAEDYSMLQHLA
                     GGRVDLMLGRGNTAPVYPWFGQDIRNGIALAIEHYALLHRLWREERVDWEGQFRTPLQ
                     SFTSTPRPLDGVPPFVWHGSIRSPEIADQAAFYGDGFFHNNIFWNIEHVQRLVGFYRE
                     RFERYGHGGADEAIVGLGGQFFARPRAEDAVREFRPYFDNAPVYGHGPSLEEFSRTTP
                     LTVGTPQQVVERYASMREYVGDYQRQLFLVDHAGLPLETVLEQVEILGTEIVPALRHE
                     MSIGRPAHVPDAPTHESLRARVAAATAAS"
     gene            2146..3933
                     /locus_tag="PLX85_00625"
     CDS             2146..3933
                     /locus_tag="PLX85_00625"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRC61709.1"
                     /translation="MSADGIGRAGSGGLAWRLHAALAVTLVVYAATYTFWTAVRPFGR
                     DSLAFLAGSDVGSLLPPLAAGALGLLASRRCSGRPRRGWLLLGLGWLAWGFGETTWAV
                     YEVILRVETPFPSLVDVGYLAAVPLMLGGIVMLMPRESDARHWADVASAGATALVGAA
                     LVWHFVVRDIFAEGDTGAVQKTLSAAYPIGDLLLLAVLALALRKVWRGAAGIILAVLF
                     IGLLAFLTADLVFAYLDLNDEYVSGNLVTDPAWVIGFLLMSYAAGLQLLFDPDYRVVL
                     SEQRRARWLRVAPALVLLVVGALFASDALGVGAGFQLDRALGFVAAVGGVSVAARVLL
                     GLTDNVEVLLDTRKELRRAADLQTTLLPAPTHASDTYQLAARYLPARNLGGDFYDWWE
                     EPAGTLHISFGDVMGKGMAAALLMASMRTALRACSVGCDAAETIAKAANLMAQDFANA
                     SAFSTLFYARFDAATHSLTYVDCGHGLAFVLTHAGEVRRLPATDLPLGTLDGAHRFTS
                     TEVRLAEGDSLFICSDGLLDLMPEIMTPAALGPLLVAPLDASQVVDHAVMTALDAGTP
                     EDDITVVLLRLDGTGRPAASVTTPAGVAS"
     gene            complement(3908..4348)
                     /locus_tag="PLX85_00630"
     CDS             complement(3908..4348)
                     /locus_tag="PLX85_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC61710.1"
                     /translation="MQLTSPPVVVRRAQLQARVDRLEGVHGALEHFWSNAEDRGAQPP
                     YNARMQFDLAVIEIAANICEHAAHGGGVTLELELSLYGDRVEARFDDDAGPASLPATS
                     EMPSQAAESGRGLAIVRRIVDELRYERVGEHHNSWFLRKQLQPE"
     gene            complement(4335..4670)
                     /locus_tag="PLX85_00635"
     CDS             complement(4335..4670)
                     /locus_tag="PLX85_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRC61711.1"
                     /translation="MRILLETPSDGAAVLHIHGRLDLTAASEVKSAILRAVDNGRPQV
                     VVDLKDVPFIDSSGLGALIGGLRAARTAGGDLRIANPGPQARMILELTTLDRVLVTHE
                     TVEAALAAD"
     gene            4924..5904
                     /locus_tag="PLX85_00640"
     CDS             4924..5904
                     /locus_tag="PLX85_00640"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HRC61712.1"
                     /translation="MKRWIALVLSSGLFVALAVMLTAGAGAADPPAAPTPTAGTKIAT
                     GFGMVLGTTVGPDNALYVADASTGSVWRVDPESGKKTLFAEGLPTSEEGGVSDIAFIG
                     FDLYALTTSVDSPGVVGIYHLPKHGKWEILADLGAYSKAHPVGFADAAPTGNPFRLDV
                     LDAGRLLVVDSNHNRLLVVETDDGDISQLAQFQNIVPTGTALADNVLYLSQIGAFPHK
                     PEDGRVWAVTLPEGEASLVAAGTPFIIDVEKGPSSRLYALSFGTQPNNPEGPPADPFS
                     GKLLLVGDGSFQILVANLTMPTSLEVIGDTAYVTTLGGDVWRIDNVSSLN"
     gene            complement(5992..6903)
                     /locus_tag="PLX85_00645"
     CDS             complement(5992..6903)
                     /locus_tag="PLX85_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HRC61713.1"
                     /translation="MRLEAVHRHPLLAYFGLTWAISWLLWLPAVASRQGWWEIDVPIA
                     WHYAGAVGPITAAIVVAFIVEGQEGVLALAQQYRPSRASLPWLVGTLGGMLVLFGAGL
                     TAARLADGEWPAYEEVANAGNLPALGLPLTFLVHLVTFGIGEETGWRGFALPRLQEQR
                     SAARATALLFVGWSLWHVPSFFENPDYTDMNTGTVVGWAIGLALGAVFLTWLYNSTRG
                     SLLTIVLWHATFNTITASEAASGALAAVATTGIMAMAVVVFLLAGPAELRGWSRRAGP
                     RVGWRQLGLEAEGARRREAATPASGVR"
     gene            complement(7004..>7420)
                     /locus_tag="PLX85_00650"
     CDS             complement(7004..>7420)
                     /locus_tag="PLX85_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRC61714.1"
                     /translation="VIASHVEMVAVNSAIEVDLTGQVCADSIGGRFYSGIGGQLDFIR
                     GAARAVQGRPIIALPSTTADGRISRIVSRLAPGAGVVTTRGDVHFVVTEHGIAELHGR
                     NVRERGQALLRVAHPAFRDQLAREAFETYGMRLSGA"
BASE COUNT         1091 a         2559 c         2524 g         1246 t
ORIGIN      
        1 gggccgtctg cgcgcggtcg ccgaagccgc gggcgccgag acgctggtgt cacagctgtc
       61 gcgcgatggc agcgatgagg atctcggcgc tgacctcgtc ggcgccgacg tgctcgtgtg
      121 cgtcggtggc gatggcacgg tcctgcacgc caccggcatc gcttcgagtg ccggcgtgcc
      181 cgtgtttggc gtgcgcatgg gtcgcctcgg ctttctggcc gaggcgactg ccggcgaggc
      241 cgaagacgcc ctcacgcgca tcatcggcgg cgaaggtcgc ctcgaacggc gcgtcatgct
      301 gcaggcccag gtcggagagg ccgcgccgca gcacgcgctc aacgacgtcg tgatcgggcg
      361 catgcacctc ggtcgcacgg tgaccgtggg cctgcgcgtc gacggcgtgc tgctggcgga
      421 gtaccgggcg gacgcggtta tggctgccac ggcgacgggg tcgactggtt actcgctctc
      481 cgttggcggg ccgattctgc atccgacgtc cgacgacctg atcgtcacgg cggtcgcccc
      541 acacctctcc tacccgaacg ccctcgtgct gcccggcgat gccagcatcg agttcgaggt
      601 cgcgcgcggc tacgaggcgg tgctgctggt cgacgggggg cacgagcagg ccgtcacgtc
      661 tggcacgttc gtccgcgtga cccggtcacc gcgcgaggcg ttgttcctcc gcctgggagc
      721 cgagcgccag ttctacgcga acctcgcgcg gcggctgggc tggctgcgca tcgaccacgt
      781 tgtcggcaac gacgaccccg ccgaggcggg tctcagctag cggccgtcgc tgccgctacg
      841 cgcgctcgca gcgactcgtg ggtcggcgcg tccgggacgt gtgcgggccg cccgatggac
      901 atctcgtgcc gcagcgccgg cacgatctcc gtgcccagga tctcgacctg ctcgagcacc
      961 gtttcgagcg gcaggcctgc gtggtcgacg aggaacaact gacgctggta gtcgccgacg
     1021 tactcccgca tcgaggcgta tcgctcgacg acttgctggg gcgtgccgac cgtgagcggc
     1081 gtcgtgcggc tgaattcttc gagggatggc ccgtgcccgt acacgggcgc gttgtcgaag
     1141 tacggacgaa actcacgcac ggcgtcctcc gcgcgcggcc gcgcgaagaa ctggccgccg
     1201 agtccgacga ttgcctcgtc cgcgccgccg tgcccgtagc gctcgaaccg ctcccggtag
     1261 aacccgacga gccgttgcac gtgctcgatg ttccagaaga tgttgttgtg gaagaagccg
     1321 tcgccgtaga acgcggcctg gtcagcgatc tccgggctgc ggatcgaccc gtgccacacg
     1381 aacggcggca cgccgtccag cggccgcggc gtcgaggtga acgactggag cggcgtccgg
     1441 aactggccct cccagtccac gcgttcctcg cgccacaaac ggtgcaacag cgcgtagtgt
     1501 tcgatcgcaa gcgcgatgcc gttccggatg tcctgcccga accaggggta caccggcgcc
     1561 gtattgccgc gcccaagcat gaggtcgacc cggccgcccg caaggtgctg cagcatcgag
     1621 tagtcctcgg cgatcttgac cgggtcgttg gtcgtgatca gcgtcgtcgc cgttgagagc
     1681 acgatgcgct tcgtccgccc cgcgatgtac ccaaggagcg tcgttggcga cgagggcacg
     1741 aagggtggat tgtgatgctc gcccgtcgcg aacacatcga ggccgacttg ttccgccttc
     1801 agtgcgatcg cgaccatcga ctggatacgt tcggcctcac tgggtgcgcg gccactgagc
     1861 gggtcgggtg tgacgtcccc aacggagaag atgccgaatt gcacgggagc cctttccgga
     1921 taggcgtggt tccattgtca cacggggcgg acgggacgcc cgaccatcac gctcgaacgc
     1981 gcttgtaatt gcgcgccgag tcgggcaagc tcccgaatgc gcgcctcacg tccagatttg
     2041 ggggttcctc ttcgggagcc ggtgataaga tcacccggct caccgcgaag gtgatcgttg
     2101 gcaggcgcct tgtccagtac ctcaccgacg gcttcgttca ctcacatgtc cgccgatggc
     2161 attggccgcg ccggttcggg cggcctcgcg tggcggctcc acgcggccct ggctgtcacg
     2221 ctcgtcgtct acgctgcgac gtacacgttc tggactgctg tgcgtccatt tggccgcgac
     2281 agcctcgcct ttctcgccgg ctctgacgtc gggagcctgc tgccgccgct ggcggccgga
     2341 gccctcggtt tgctcgccag ccgccgctgc tcggggcgcc cccgccgagg atggctgctg
     2401 cttggactcg gctggctcgc ctggggattc ggcgaaacca cgtgggccgt gtacgaggtc
     2461 atcctgcgcg tcgagacgcc gttcccctcg ctcgtcgacg tcggatacct ggccgcggta
     2521 ccgctcatgc tcgggggcat cgtcatgctg atgccgcgcg agtcggacgc ccgtcactgg
     2581 gcggacgttg cgagcgccgg cgcgaccgcc ctggtcggtg cggccctcgt ctggcatttc
     2641 gtcgtcaggg acatcttcgc ggaaggcgac acgggcgctg tccagaagac actgtcggcc
     2701 gcctatccca tcggcgacct gctcctcctg gcggtgctgg cgctcgcgct gcgcaaggta
     2761 tggcgcggcg cggcgggcat catcctggcg gtgctcttca tcgggctgct cgcctttctc
     2821 accgccgacc tcgtgttcgc ctacctggac ctgaacgacg agtacgtctc gggcaacctc
     2881 gtcactgacc ccgcctgggt gatcgggttc ctcctgatga gctacgccgc gggcctccaa
     2941 ctgctgttcg atcccgacta ccgagtcgtg ctctccgagc agcgtcgtgc tcgttggctg
     3001 cgtgtggctc ccgctctggt gctcctcgtg gttggcgcgc tgttcgcgag cgatgcgctc
     3061 ggggtcggtg ccggatttca gctcgaccgc gcgttggggt tcgtcgccgc ggtcgggggc
     3121 gtgagcgtcg ccgcgcgcgt cctgctcggg ctcaccgata acgtcgaggt cctgctcgac
     3181 acgcggaagg aactgcggcg tgccgccgac ctgcagacca cgctcctgcc cgcgcccacg
     3241 cacgcgagtg acacctacca gctggccgcg cggtacctcc ccgcaaggaa cctcggcggc
     3301 gacttctacg attggtggga ggagcccgcg gggacgcttc atatctcgtt cggcgacgtg
     3361 atgggcaaag gcatggcggc cgccctgctc atggcctcca tgcgcaccgc gctgcgggct
     3421 tgttcggttg gttgcgatgc cgcggagacg atcgcgaaag cggcgaacct gatggctcag
     3481 gacttcgcaa atgcttcagc gttcagcaca ttgttctacg ctcgcttcga cgcagcgacg
     3541 cattcgctga cgtatgtcga ttgcggccac ggcctggcct tcgtcctcac gcacgcgggc
     3601 gaggtgaggc ggctgccggc gaccgacctg cccctcggca cgttggacgg cgcgcaccgc
     3661 ttcacgagta ccgaggttcg gctggccgag ggagactcgc tgttcatctg cagcgatggg
     3721 ctgctggacc tgatgcccga gatcatgacg cctgcggcgt tgggcccgct gctcgtggcg
     3781 cccctcgatg cctcacaggt cgtcgaccac gccgtcatga cggcgctgga cgcgggcacg
     3841 cccgaggacg acatcaccgt ggtgctgctc cgcctcgatg gcacggggcg gcctgcagcc
     3901 tcggtcacta ctccggctgg agttgcttcc tgaggaacca gctgttgtgg tgctcgccca
     3961 cccgttcgta acgcaactcg tcgacgatcc tgcggacgat tgccagtccg cggcccgact
     4021 cggccgcctg ggacggcatt tcgctcgtcg cgggtaagct cgccgggcct gcatcgtcgt
     4081 cgaatctggc ctcgactcgg tcaccgtaga ggctcaactc gagttcgagc gtgaccccgc
     4141 cgccgtgcgc ggcatgctcg cagatgttgg ccgcgatttc gatcactgcg aggtcgaact
     4201 gcatccgcgc gttgtacggc ggctgtgctc cacggtcctc ggcattcgac cagaagtgtt
     4261 cgagcgcgcc atggacgccc tcgagccggt cgacccgcgc ttgcagttgg gcccggcgca
     4321 cgaccacggg ggggctagtc agctgcaagg gccgcctcaa ccgtctcgtg cgtgacgagc
     4381 acgcggtcga gcgtggtcag ctcgaggatc atgcgtgcct gggggcccgg gtttgcgatg
     4441 cggaggtccc cgccggcggt gcgagcggcg cggagtccgc cgatcaacgc tcccaggccg
     4501 gagctgtcga taaagggcac gtccttcagg tcgacgacca cctgcggccg cccgttgtcg
     4561 acggcgcgca ggatcgcgct cttcacctcc gaggcggcgg tcaggtccag gcgaccgtgg
     4621 atgtgcagaa cggccgcgcc gtcggatgga gtctcgagca ggatgcgcat gcggtccccc
     4681 ttcctcgggc gagagcattc cggagcctgc gcattcgtgg tgccactacc ttacagccgc
     4741 cgggcgcctc atgtcccgct gtgcacgcga cgctctgccg gattgagagc caatcgcagt
     4801 cggtatcacc cggatcgagg gtggtccggc gaccgggggc gagacatcac ggcagggatg
     4861 gtgcgtatgc tcacgcgaac ggcataatgc gcaagagcgt ccgcagttaa gcaaggaggg
     4921 cttatgaaac gttggatagc gctcgtccta tcatccgggc tctttgtggc actggcggtc
     4981 atgctgaccg ctggcgccgg ggcggccgat ccgcccgccg ctcccacgcc aaccgccggc
     5041 acgaagatcg cgactggctt cggcatggtg ctcggcacca cggtcgggcc ggacaacgcg
     5101 ctctacgtcg ctgatgcctc cacgggctcg gtctggcgcg tcgatccgga gagcggcaag
     5161 aagaccctgt tcgctgaggg cctccccacg agcgaggagg gcggcgtctc cgacatcgcc
     5221 ttcatcggct tcgacctgta cgccctgacg acaagcgttg actcgcccgg cgtggtcggc
     5281 atctaccacc tgccgaagca tggcaagtgg gagattctcg cggacctcgg cgcgtactcg
     5341 aaggcgcacc ccgtcgggtt cgccgacgcc gcgccgactg gcaacccgtt ccgcctcgac
     5401 gtcctcgacg cgggccgcct gctcgtggtg gactcgaacc acaaccgcct gctcgtcgtg
     5461 gagaccgacg acggcgacat ctcgcagctc gcacagttcc agaacatcgt gcccaccggg
     5521 acggcgctcg cggacaacgt gctctacctc tcccagatcg gcgccttccc gcacaagccg
     5581 gaggacggtc gcgtctgggc cgtgaccctg cccgagggtg aggcgtcgct ggtcgccgcc
     5641 ggcaccccgt tcatcatcga cgtcgagaag ggcccctcaa gccggctcta cgcgctgtcg
     5701 ttcggcacgc agcccaacaa cccggaaggg cctccggctg acccattcag cggcaagctg
     5761 ctgctggtcg gcgacggttc cttccagatc cttgttgcca acctgacgat gccgacctcg
     5821 ctcgaggtga tcggtgacac cgcctacgtc accacgctcg gcggcgacgt ctggcgcatc
     5881 gacaacgtga gcagcctcaa ctagccgaag cgcgcttcag catggttgat gcgagagggg
     5941 cggccatctg ggccgcccct ctcgacgttt gagcgagcga aaaccgggcg tctagcggac
     6001 gccgctggcc ggggtggcag cttcccggcg ccgggcacct tcggcttcga gcccgagctg
     6061 gcgccagccc acccgtggcc cggctcgacg agaccagccg cgcagttccg ctgggcccgc
     6121 gagcaagaac acgaccaccg ccatggccat gatccctgtg gtcgccacgg ctgcgagggc
     6181 ccccgaggcc gcctcggacg ctgtgatggt gttgaacgtg gcatgccaga ggacaatcgt
     6241 cagcaggctg ccgcgcgtgc tgttgtacaa ccaggtgagg aagaccgcgc caagtgcgag
     6301 accgatcgcc cagccaacga cggtgccggt gttcatgtcg gtgtagtccg ggttctcgaa
     6361 gaacgatggc acgtgccaca gtgaccagcc gacgaacagc agcgccgtcg cgcgggcagc
     6421 gctgcgctgc tcctgcagcc ggggcaacgc gaatccccgc cagcccgtct cttcgccgat
     6481 gccgaaggtg accagatgga cgaggaaggt caacggcagc cccagggccg gcaggttccc
     6541 ggcgttcgcg acctcctcgt aagcgggcca ttcgccgtcg gcgagtcggg cagccgtcag
     6601 accggctccg aagagcacga gcatgccgcc cagggtgccc acgagccacg gcagtgaggc
     6661 gcgagacgga cggtattgct gagcgagggc gaggacgccc tcttggccct ccacgatgaa
     6721 tgcgaccacg atggccgccg tgattggccc caccgccccg gcgtagtgcc aggcgatggg
     6781 cacgtcgatc tcccaccagc cctgccgcga ggcgactgcg ggcagccaga gcaaccatga
     6841 gatcgcccac gtgagcccga agtaggcgag cagcggatgg cggtggacgg cttcgaggcg
     6901 catgtgtgcc tcctcgagat acccctcgat tctggccggc cccccaatgc gaggtagagt
     6961 cgttggcctt cccgtgggaa ggacgaaggt cgcaccaagg acctcaggcg ccgctgagcc
     7021 gcatcccgta tgtctcgaat gcctcacgag cgagctggtc gcggaacgct ggatgggcga
     7081 cccgcaacaa cgcctgtccg cgttcccgga cgttccggcc gtgcagctcg gcgatgccgt
     7141 gctcggtgac cacgaagtgc acgtcgccgc gcgtggtgac cacgcccgcg cccggcgcca
     7201 ggcggctcac gatgcgcgag atgcgcccgt cagcggtggt cgacggcagc gcgatgatcg
     7261 gccgtccctg cactgctcgc gcggccccac ggatgaagtc gagctgcccg ccgatgccgc
     7321 tgtagaaccg tccgccgatg gaatcggcgc acacctgtcc cgtcaagtcc acctcgatcg
     7381 ctgagttcac ggcgaccatc tcgacgtggc ttgcgatgac
//
LOCUS       DAORPG010000013         7404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_443_length_7404_cov_3.482379, whole genome shotgun sequence.
ACCESSION   DAORPG010000013 DAORPG010000000
VERSION     DAORPG010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7404
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_443_length_7404_cov_3.482379"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="PLX85_00655"
     CDS             <1..804
                     /locus_tag="PLX85_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61715.1"
                     /translation="AAGGSNEAVRVAKGLAEQVVTRAETVYPIPDGLDSGAALHLGIS
                     YGTAYGALHWRARLQPGETLLVLAAAGAVGLASVYDRVPKLEPWRYSVPLSVALILLS
                     AGALLYGPAATVARSREGQHRGLDGLAYLSSDPGLVAAINWAQANLDHHDVLLEAVGA
                     DYQGGNIVSAATGIPTLIGWPGHQIQWRGKIPAIAERENVVARVYQEGATEDVRALAR
                     RYGVTHVYIGSEERTQFGANVAARFDAWPAVFDAPGVRIVAVPEAGANP"
     gene            801..2765
                     /locus_tag="PLX85_00660"
     CDS             801..2765
                     /locus_tag="PLX85_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HRC61716.1"
                     /translation="MSVRRIRVERPDTVTWIVLGLVALALVLRLYDLGARALHHDESL
                     HATYSWYFAADRHVSNCAAAYCHDPLMHGPFQFHFMALMFKLFGDGDFMARVPAALTG
                     TALVATPLLLRRWLGTIGTVAAALFITLSPSLLYYSRFAREDIYMWMWTVLLFVSVWR
                     YLDDGRDRWLGIAAAALALSFATKESVYLTIAIVLVYLDVRLTIEFLDQRGTKGEERW
                     LEGLFLAPVAWLLAAFWQPLESWRTSRGFTTLPRAADMLIVIGILALPQLSAAAQIPL
                     KAMGVDVVGDTERQIGIITVCALLGGATIVGLIWDWQRWVLVAAIFYAITIPLFTTEF
                     LNTRNGLASDFWGALDYWMAQQEVQRGTQPWFYYLMMVPLYEFLTLIPALLGGVWLIV
                     KRRDGLAVLFGWWFLGSLLAFSYAGEKMPWLTTHMAVPLAFMAAYVIGRYLPAIIVRL
                     RDLERSSSSTWAVAAVAGFAFLLAFGLSVRNAHAVSFLHPDTPVEPLIYTQTSRDVPN
                     LSRDIYKLAAEAPPGQLSIFVDSSDGATWPWAWYLRHLNVAYADPAYIQQYATPKTLI
                     LALPNTLSSVPQLREQAGEVRPYHHRWAFPEDGYKGTTWSSFLTRLSNGRLEQQWYEF
                     MLHRIPEQWVLGWSLDGIALIPSLDTLNQN"
     gene            2813..3832
                     /locus_tag="PLX85_00665"
     CDS             2813..3832
                     /locus_tag="PLX85_00665"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRC61717.1"
                     /translation="MGAAMLRNRRVWIGLVVSIVFLGLLLRSVDRHELANALEEIDPL
                     WLVAAFAVELAALWFRALRWRLILDSHVRIKTQDAFSVVIIGYAANNLLPVRAGELVR
                     AQLLYDSHGASRLGTLGTIVVERIFDGLVLALFLAATIALAGGSDFLRVVAALMTAAF
                     LVATVVLALLALQPRGSDIWTIRLLRLVPATIRPRARVWLGSFLAGLGGLRGQRAWGL
                     VIGTTFISWGLEAVCYWLVGIGFGLDIDFPLYLAVTGAANLAIAAPSTAGGVGPFEFF
                     AQKALTAFGVSAAAATVYALVLHALLLIPVVLLGIALLWRRHLGLGEMLRAGQGTPAE
                     GVADS"
     gene            3829..5199
                     /locus_tag="PLX85_00670"
     CDS             3829..5199
                     /locus_tag="PLX85_00670"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRC61718.1"
                     /translation="MKVVIIGGGIGGLAAGLDLTRAGHEVRVYEASDVLGGQVRTFEV
                     GGGRLEIFYHHIFHSDTFVTETVNELGLGKDLEWIESKVGFLSEGRIYPFVGALDLLR
                     FKPVSLLTRVRLGLAALYLRRQSDWKRYEGKRAKPWIERYVGREGYNRVWGPLLRAKF
                     GDHADDISMTWFWGKIYLRFASRPGGLFAREKLGYLRGSFGRLVDRMGEAVEERGGIV
                     RTSCPVHEVLSKDNRVTGVRLGGPDGEVVPADAVIATTPSQIFERTVPNLDPEYVRRL
                     DSIDYQWATVFVLALDRPLSSTYWLTVTDPDCPFVVAIEQTNFRSPSEYGGQHVVYFS
                     NYTDPGDPIVDENEEQVLDRYEAYIRRINPAFDRSWIKGKWLFKDRAGQPIVHYRYHE
                     SIPPLATGIEGLYLANTTQIYPEDRGQNYSIRIGKQVAKLAMEDYGARLGAAHAHSEE
                     EASTQP"
     gene            5282..5773
                     /locus_tag="PLX85_00675"
     CDS             5282..5773
                     /locus_tag="PLX85_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRC61719.1"
                     /translation="MPLETEATAEIRALVADLRPDDADMLEALHRVQHRYGYVSQEAM
                     YVIADQLRVTPAHVYGVTTYYSDFRTTPPPEVTVAWCGGPACRLKNGAGIRDAMQAVL
                     GIELGEDTADGRVGLVAGQCNGSCEQAPQVWVGERVVGRLTAARAVTLARALRDGADP
                     ASV"
     gene            5766..>7404
                     /locus_tag="PLX85_00680"
     CDS             5766..>7404
                     /locus_tag="PLX85_00680"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRC61720.1"
                     /translation="MSDAYDTLRERADAAWAAWQHPERPRIDIAIETSSLAGGAGDTL
                     RALEAEIGRRGAAVDVGRVHGYGLQWLQPLADITWPDGTRVLYGPVTAEDVGLLVDEA
                     TGLVGAAANLAVGTLSGRRTGIPPIADHPFLAAETMGRRLLARIGHTDPESLDHYIAT
                     GGYFALDRMLSRHQDPASVRQLMIDANLTGRGGAWFPAGMKWNFLAGAAVEERYLICN
                     ADEGDPGGWVNRVLLEGDPHLIVEGMLTAAFATGARHGFFYIRNEYPLAVQRVRQAIA
                     DATAAGLLGDNILGSGFSCTLEVIRGAGAYVCGEETGLISSVQDGRGMPRVKPPFPAA
                     AGVFMKPSNVNNVETYANVPLILRNGAEWFRQAANPAGTDIGTKIFSFSGDIARVGFM
                     EVPFGVPLAKIIEVCGGMVGGGKLKAIQAGGPLAGYLPGSLLGELSLERAPFSKHSAL
                     MGGGGIVFIGEGSCSVDLNVMFSEFLEEESCGRCTTCHHGNQRMHEVFSRVAAGGGRT
                     EDRHNLELVGSSLAYSNCVHGQASPTIMRNTLRLFERE"
BASE COUNT         1056 a         2828 c         2258 g         1262 t
ORIGIN      
        1 gcggccggcg gctccaacga agcggttcga gtcgccaagg gcctggccga gcaggtggtg
       61 acgcgcgccg agacggtcta cccgatcccc gacggcctcg attccggcgc cgcgctgcac
      121 ctgggcattt cctacggcac cgcctacggc gcattgcact ggcgcgcgcg actgcagccg
      181 ggagagacgc tgctagtgct ggccgcggcg ggcgccgtcg gcctcgcctc cgtctacgac
      241 cgcgtcccga agctcgaacc ctggcgctat tcggtcccgc tctccgtggc gctgatcctg
      301 ctctccgccg gcgcactcct ctacggtccc gccgcgaccg tcgcccgctc acgcgagggt
      361 cagcatcgag gtctcgacgg cctcgcctat ctcagctccg accccggcct cgtcgcggcc
      421 ataaactggg cccaggcgaa tctcgaccac cacgacgtgc tgctcgaggc cgtcggcgcc
      481 gactaccagg gcggcaacat cgtctcggcc gccacgggta ttcccacgct catcggctgg
      541 cccgggcacc agattcaatg gcgtgggaag atacccgcaa tcgccgaacg cgaaaacgtc
      601 gtcgcgcgcg tctaccagga gggtgctacc gaggatgttc gtgcccttgc gaggcgctac
      661 ggggtgacgc acgtctacat cggcagcgag gaacgcaccc agttcggcgc caacgtcgcc
      721 gcgcgcttcg acgcctggcc cgccgtcttc gatgcgcccg gcgtccgcat cgtcgccgtc
      781 cccgaggcgg gggcaaaccc gtgagcgtcc ggcgcatccg cgttgaacgg cccgacacgg
      841 tcacctggat cgtgctcggc ctcgtagcgc tcgcccttgt gctgaggctg tacgacctcg
      901 gcgcgcgcgc tctccatcac gacgagtcgc tgcatgccac ctattcgtgg tacttcgccg
      961 ccgaccgcca cgtttcgaac tgcgcggccg cctactgcca cgacccgctc atgcacgggc
     1021 ccttccagtt ccacttcatg gcgctcatgt tcaagctctt cggcgacggc gacttcatgg
     1081 cacgcgtccc cgccgccctc accgggacgg ccctcgtcgc cacgccgctg ctcctgcgcc
     1141 ggtggctcgg cacgatcggg acggtcgcgg cggcgctctt catcaccctc tcgccctcgc
     1201 tgctctacta ctcgcggttc gctcgcgagg acatctacat gtggatgtgg accgtcctcc
     1261 tcttcgtctc cgtctggcgc tacctcgatg acggccgaga ccgctggctc ggcatcgctg
     1321 ccgccgccct cgccctctcc ttcgcgacca aggagtccgt ctacctcacg atcgcgatcg
     1381 tgcttgtcta cctcgatgtc cgcctgacca tcgagttcct cgaccagcga ggtacgaagg
     1441 gtgaggagcg ctggctcgag ggcctcttcc tcgcacccgt cgcctggctg ctcgccgcct
     1501 tttggcagcc cctcgagtcg tggcgcacct ctcgaggttt caccacgctc cctcgtgcgg
     1561 ccgacatgct catcgtcatc ggcatcctgg ccctgcccca gctctccgcg gcggcgcaga
     1621 ttccactgaa ggcgatgggc gtggacgtcg tgggcgacac cgagcgccag atcggcatca
     1681 tcaccgtctg cgccctcctc ggaggcgcga ccatcgtcgg gctgatctgg gactggcagc
     1741 gttgggtgct ggtcgctgcc atcttctacg cgatcacgat ccccctcttc accaccgagt
     1801 tcctcaacac gaggaacggt ctcgcctccg acttctgggg cgccctcgac tactggatgg
     1861 cccagcagga ggtccagcgc ggcacccagc cgtggttcta ctacctgatg atggtcccgc
     1921 tgtacgagtt cctgacactg atacccgccc tgctcggagg cgtctggctc atcgtcaagc
     1981 gacgcgacgg cctggcggtc ctcttcggct ggtggttcct cggcagcctc ctcgccttca
     2041 gctatgccgg cgagaagatg ccctggctca ccacgcacat ggccgtcccg ctcgcgttca
     2101 tggccgcgta cgtcatcggt cgctacctac cggcgatcat cgtgcgcctg cgtgacctcg
     2161 aacggtcgtc gtcctcgacc tgggcggtcg ccgcggtcgc cggcttcgcc ttcctgctgg
     2221 cctttggcct gtccgtacgc aacgcgcacg ccgtgtcgtt cctccaccct gacacgccgg
     2281 tggagccgct catctacact cagaccagcc gagacgtccc gaacctctcg cgcgacatct
     2341 acaagcttgc ggccgaggcg cctcccggcc agctttcgat cttcgtcgac agcagcgacg
     2401 gggcgacatg gccctgggcc tggtacctgc gtcacctcaa cgtcgcctac gccgaccctg
     2461 cgtacattca gcagtacgcc acgcccaaga cgctcatcct cgcgctcccc aacacgctct
     2521 cgtcggtgcc tcagctccgc gagcaggctg gcgaggtgcg cccgtaccac caccgctggg
     2581 cgttcccgga ggacggctac aagggcacca cctggtcgag cttcctcacc aggctctcca
     2641 acggccgtct cgagcagcag tggtacgagt tcatgctcca ccgcatcccg gagcagtggg
     2701 tgctcggctg gtcactcgac ggcatcgccc tgattccctc gctcgatacc ctgaatcaga
     2761 actaaaacgt ctgcggctgg cccgcaacga actcaacgag gaacatgtcc ccatgggtgc
     2821 tgcgatgctg cgcaaccgcc gcgtatggat cggcctcgtc gtcagcatcg tcttcctcgg
     2881 actcctcctc cgctccgtcg accgtcacga actcgcgaac gcgctcgagg agatcgaccc
     2941 gctctggctc gtcgcggcct ttgccgtcga gctggccgca ctctggttcc gagcgctccg
     3001 ctggcgcctg atcctcgatt cccatgtccg catcaagacg caggacgcct tctcggtcgt
     3061 gatcatcggc tacgccgcca acaacctgtt gcccgtccgc gccggcgaac tggtgcgtgc
     3121 gcaattgctc tacgacagcc acggcgccag ccgccttggc acgctcggca cgatcgtcgt
     3181 cgagcgcatc ttcgacggcc tggtgctggc gctcttcctc gcggcgacga tcgcgctcgc
     3241 cggcggcagt gacttcctgc gggtggttgc cgcgctgatg accgccgcct tcctcgtcgc
     3301 gaccgtcgtc ctcgcgctgc tcgcgcttca gccccgaggc tcggacatct ggaccatccg
     3361 cttgctgcgc ctcgtgcccg cgacgatccg tccgcgcgcg cgcgtctggc tcggcagctt
     3421 cctcgccggc ctgggcggcc tccgaggtca gcgggcctgg ggcctggtca tcggcaccac
     3481 gttcatctcc tggggcctcg aggcggtctg ctactggctc gtcggtatcg gattcggcct
     3541 cgacatcgac ttcccgctct acctcgccgt caccggggcc gcgaacctcg ccatcgccgc
     3601 ccccagcacc gccggcggcg tggggccctt tgagttcttc gcgcagaagg cgctgacggc
     3661 gttcggcgtc agcgccgcgg ccgcgaccgt gtacgccctg gtgctgcacg ccttgctgct
     3721 gatcccggtc gtgctgctgg gcatcgcgct gctgtggcga cggcacctcg ggctcggcga
     3781 gatgctgcgg gccggccagg gcacgcccgc ggaaggggtg gctgactcgt gaaggtcgtg
     3841 atcatcggtg gcggcatcgg cgggctcgcc gcgggcctcg acctcactcg cgccggtcac
     3901 gaagtccgcg tctacgaggc gagcgacgtc ctcggcggcc aggtgcgcac cttcgaggtc
     3961 ggcggaggcc gcctcgagat cttctaccac cacatcttcc actcggacac gttcgtcacc
     4021 gagaccgtga atgagctcgg gcttggcaaa gacctcgagt ggatcgagtc gaaagtcggc
     4081 ttcctctcgg agggccggat ctacccgttc gtcggcgcgc tcgacctgct ccgcttcaag
     4141 cccgtctcgc tgctcacccg cgtccgtctc ggccttgccg cgctctacct ccgccgccag
     4201 tcggactgga agcgttacga aggcaagcgc gcaaagccgt ggatcgagcg gtacgtcggc
     4261 cgcgaggggt acaaccgcgt ctggggtccg ctccttcgcg ccaagttcgg cgatcacgcc
     4321 gatgacatct ccatgacctg gttctggggc aagatctacc ttcgcttcgc gtcgcgcccc
     4381 ggcggcctct tcgcccgtga gaagctcggc tacctccgcg gctccttcgg gcgcctcgtc
     4441 gaccgcatgg gcgaggctgt cgaggaacgc ggcggcatcg tgcggacgag ctgccccgtc
     4501 cacgaagtgc tctccaagga caatcgcgtg accggcgtcc gcctcggtgg gcccgacggc
     4561 gaggtcgtcc cggccgacgc cgtcattgcc accacgccct cgcagatctt cgagcgcacg
     4621 gtcccgaacc tggaccccga gtacgtccgc cgcctcgaca gcatcgacta ccagtgggcg
     4681 acggtgttcg tgctcgccct cgaccgcccg ttgtcctcga cctactggct gaccgtcacc
     4741 gatcccgact gcccgttcgt cgtcgccatc gagcagacca acttccgctc tccctcggag
     4801 tacggcggtc agcacgtcgt ctacttctcg aactacaccg accccggcga ccccatcgtc
     4861 gacgagaacg aggaacaggt cctcgaccgc tacgaggcgt acatccgccg tatcaatccc
     4921 gccttcgacc gctcctggat caagggcaag tggctcttca aggaccgcgc cggccagccc
     4981 atcgtgcatt accgctacca cgagtcgatc ccgccgctcg ccaccggcat cgagggcctc
     5041 tacctcgcca acaccacgca gatctacccg gaagaccgag gccagaacta ctcgatccgc
     5101 atcggcaaac aggtggcgaa actcgcgatg gaggactacg gcgcgcgcct cggggcggcg
     5161 cacgcgcact ccgaggagga ggcctccacc cagccttgaa ccccccccgg tgcgctccta
     5221 atatgaaccc cgatcctgcg gcttacgccg cacctcctcc ggctgcacga tcggatgcgc
     5281 gatgccgcta gagaccgaag cgactgccga gattcgcgcg ctcgtcgccg acctccggcc
     5341 cgacgacgcc gacatgctcg aggccctcca ccgcgtccag caccgctacg gctacgtctc
     5401 ccaggaggcg atgtacgtca tcgcggacca actgcgggtc actcccgccc acgtttacgg
     5461 cgtcacgacc tactacagcg acttccgcac caccccgccg cccgaggtca ctgtcgcctg
     5521 gtgcggcggc cccgcctgcc gcctcaagaa cggcgcgggc atccgcgacg ccatgcaggc
     5581 cgtcctcggc atcgaactcg gcgaggacac cgcggacggc cgtgttggtc tcgtcgccgg
     5641 ccagtgcaac ggcagctgtg agcaggcccc acaggtgtgg gtcggggagc gcgtcgtcgg
     5701 tcgcctgacc gccgcgcgcg ccgttaccct cgcccgtgcc ctgcgcgatg gagcggaccc
     5761 cgccagtgtc tgacgcttac gacacccttc gcgaacgcgc cgacgccgcc tgggccgcct
     5821 ggcagcaccc cgagcgcccc cgcatcgaca tcgccatcga gacctcgtcg ctcgccggcg
     5881 gcgcgggcga cacactccgc gccctcgagg ccgagatcgg ccgccgaggt gctgcggtcg
     5941 atgtcggtcg tgtgcacggc tacggcctac agtggctgca gcccctcgcc gacatcacgt
     6001 ggcccgacgg cactcgcgtc ctctacggcc ctgtgaccgc tgaggacgtc ggcctcctgg
     6061 tcgatgaggc gacgggcctg gtcggggccg ccgcgaacct cgcggtcggc acgctgagcg
     6121 ggcggcggac cggcatcccg ccgatcgccg atcacccctt ccttgccgcc gagaccatgg
     6181 gccgccgcct ccttgcgcgc atcggccata ccgaccccga gtcactcgac cactacatcg
     6241 ccaccggcgg ctacttcgcc ctcgaccgca tgctgagccg ccaccaggac cccgcgtctg
     6301 tgcgccagct catgatcgac gccaacctca ccgggcgcgg cggggcgtgg ttccccgccg
     6361 gcatgaagtg gaacttcctc gccggtgcgg ccgtcgagga gcgctacctc atctgcaacg
     6421 ccgacgaggg cgaccctggc ggctgggtaa accgcgtcct cctcgagggc gacccccacc
     6481 tgatcgtcga gggcatgctc acggccgcct tcgcgaccgg cgcccgccac ggcttcttct
     6541 acatccgcaa cgagtatccg cttgccgtcc agcgcgtccg ccaggccatc gccgacgcca
     6601 ccgccgcggg cctccttggc gacaacatcc tcggcagtgg cttctcctgc acgctcgagg
     6661 tgattcgtgg cgccggcgcg tatgtgtgtg gcgaggagac ggggctcatc tcctcggtgc
     6721 aggacggccg cggcatgccc cgcgtgaagc cgccgttccc ggcggcggcg ggcgtcttca
     6781 tgaagccctc gaacgtcaac aacgtcgaga cctacgccaa cgtccccctc atcctccgca
     6841 acggcgcaga gtggttccgc caggcggcga acccagccgg caccgacatc ggcaccaaga
     6901 tcttctcctt ctccggcgac atcgctcgcg ttggcttcat ggaagtcccg ttcggcgtcc
     6961 cgctcgcgaa gatcatcgag gtatgcggcg gcatggtggg cggcggaaag ctcaaggcaa
     7021 tccaggccgg cggccccctg gcgggctacc tgcccggctc actgctcggt gagctgagcc
     7081 tcgagcgagc gccgttctcg aagcacagcg ccctcatggg cggcggcggc atcgtgttca
     7141 tcggcgaggg ctcctgctcc gttgacctca acgtgatgtt ctccgagttc ctcgaggagg
     7201 agtcgtgcgg ccgctgcacg acctgccacc acggtaacca gcgcatgcac gaggtgttca
     7261 gccgcgtcgc cgccggcggc ggacgcaccg aggatcgcca caacctcgaa ctggtcggct
     7321 cctcgctcgc ctactcgaac tgcgtccacg gccaggccag ccccaccatc atgcgcaaca
     7381 ccctccgcct cttcgagcgc gagt
//
LOCUS       DAORPG010000014         7339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_451_length_7339_cov_3.627128, whole genome shotgun sequence.
ACCESSION   DAORPG010000014 DAORPG010000000
VERSION     DAORPG010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_451_length_7339_cov_3.627128"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PLX85_00685"
     CDS             complement(<1..243)
                     /locus_tag="PLX85_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRC61721.1"
                     /translation="MRFDVLTLFPGMFAGPLDESILRRARDAGLIEVVLHDLRGWATD
                     RHRTVDDYPFGGGAGMVMKPGPLFDAVEAIQPLVEPH"
     gene            complement(288..1268)
                     /locus_tag="PLX85_00690"
     CDS             complement(288..1268)
                     /locus_tag="PLX85_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC61722.1"
                     /translation="MSVAAGQYTAEAEAVEQYERQAARNPWLLLWGRLRKKRLAMLSL
                     AIVLVIYGAGIAAPLLAPYSYTEQNLERGLEGPSADHWLGTDRLGRDMLSRVLYSART
                     TLIVTVAVVLSGTLILGNFLGLLAGYRGGWIDSLIMRVGDVFASLPGLPMLILLNATL
                     LPRVLQWVGWVEDHTPISGLSSSGTASYFTVFGALALFSWVGTARLIRAQVLQLRERD
                     FILAAQATGGSTWHIIWRHLMPNVSFLIILEVSAVLGSVAGSEILLTWFGVGVQPPNP
                     SFGAMLFEGSGARTFQAHPHLLLVPAAVVTSLMFAFALLGDALNDAVRGR"
     gene            complement(1265..2251)
                     /locus_tag="PLX85_00695"
     CDS             complement(1265..2251)
                     /locus_tag="PLX85_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC61723.1"
                     /translation="MAGYIIRRLLWLPVTLLAVSFLTFSIARFGPGDPVSVAAGQIRD
                     PEVLARIRAERGLDGSIFEQYAKWVDRAVRGDFGESFAQRGYSVREIIFPKMWVSAQL
                     GLVALFVVFGLGIPLGLLSAWLHGTWLDPLIISVLLFLRALPVVVVIPPLIWFFAVQN
                     SILPVGGWDGIFDIWWIAGVIAVPIPDPHLYIPLVALTLPGFAGVARLVRVTALEVYT
                     EDYVRTARAKGLPETRVAVRHVLPNSLLPIVTVVGFALVGIVEGAFFTETLLGIPGIG
                     RLTFDSVVSRDYDVILATTVIVAFLFVVMNLLVEIVYSWIDPRVRLGEAAVV"
     gene            complement(2261..3913)
                     /locus_tag="PLX85_00700"
     CDS             complement(2261..3913)
                     /locus_tag="PLX85_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRC61724.1"
                     /translation="MGGNNRILAILLGVLAGLVLIVGGLSAILLLSGGGKDASSSNDS
                     GSKSGGSTTAATSGNLRLGGSDPISLDPAIAGDAGSATYIVEIFSGLMTLNPNLEIVP
                     DLAESFDVSADGKVYTFKLRDNAFFHNGRQVKASDVKCSMERAASRELGSSTAAAYLN
                     DIVGAKERMAGAAKDISGIEVIDDRTVRITIDQPKPYFLAKLTYPTAFVIDCQQAQSN
                     PRNWTRQPNGTGPYKLREWRLGERIVLEANSRYHLGAPKVQQVVYELAGGSQLTRFEN
                     NELDVAGVSVNDIDRVRDQNNALNKLYVRTPEFSLAYIAFSTTTPPFDDPNVRRAFGL
                     AIDRKRVADVTFSGMLTPATGILQPQLPGYTPDDKTLPFNPDEAKRALAASKYAGKMP
                     QITLTEIGGGAEGSVDTQAFIEQWKNVLGVDVQIRQSDAATFFADVDAGKLQMFSSGW
                     IMDYPDPENILDLKFYSKSSLNDIEYSNPQLDKLLEQARVERDPAARLKLYQDAEKLI
                     LGDAAILPLYFSQNHVVVNKAIKGWIEPPMIIPRLRFVTVER"
     gene            complement(4003..5805)
                     /gene="aspS"
                     /locus_tag="PLX85_00705"
     CDS             complement(4003..5805)
                     /gene="aspS"
                     /locus_tag="PLX85_00705"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRC61725.1"
                     /translation="MMRTHRCGELRAGDAGTPVILAGWVHRRRDHGGLIFIDLRDSSG
                     LVQVVFNPQIAPEASAVAAEVRNEFVLLVEGEVQPRRAGTENSDLPTGAIEVVAARVE
                     VLNPAETPPFPVNQDTPVDELTRLRYRYLDLRRERMAANLRLRHRVVKFMRDYLDGRD
                     FIEVETPVLNLATPEGARDYLVPSRVHAGSFYALPQSPQQWKQLLMVAGFEKYFQIAR
                     CYRDEDLRADRQPEFTQLDVEMSFVEEEDILALIEGLYTELAANVGPQFEVPSPFLRL
                     TYREAIERFGSDKPDLRYGMEIADLTSLVADTEFAVFRNATEAGGRIRGIAVPGGADT
                     PRRGLDALTELARENGAGGLVTVAFTGEGSLDGLTAEDVRSPVARFFTPEQFAALGRA
                     CGASRGDLLLIAADRDAVTSRVLDVLRRRVAEDRDLADPGQLIFAFITQFPMFEWDED
                     EQRWSAVHHLFTAPFAEDVELMDTDPGAVRSHAYDIVCNGQEIGSGSIRIHRRELLMR
                     VLARLGMAEEEATTKFGHMLEAFTYGAPPHGGIAPGIDRTVALFAGERDIREVIAFPK
                     TKSASDPMTGAPSPVSERQLRDVHIALDLRAARS"
     gene            complement(5888..6346)
                     /locus_tag="PLX85_00710"
     CDS             complement(5888..6346)
                     /locus_tag="PLX85_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HRC61726.1"
                     /translation="MELPKQQSIDKMWKYAEKFAEKSGTRLHPDRSITEAVVLGLAHN
                     VDEYGRPICPCNFYPTKDEDGNWPEGLYLPREEEAKRRTWICACDEMQKFKYCHCLLF
                     VNEDGLPITEYLPEDHEGREIYGLIEDPTPDKGRALGRIEEARRAEAAGD"
     gene            complement(6394..6981)
                     /locus_tag="PLX85_00715"
     CDS             complement(6394..6981)
                     /locus_tag="PLX85_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRC61727.1"
                     /translation="MEPIDDVMQLFRHQRAVRAWSDRPVSDDVLRQVLEAAIHGPSGS
                     NSQPWRFLVIRDQAVKEQVSALYEEAMAEQYQGAEPDRARDRQTLADAPVLIVPLVRV
                     PGNGRAGFQTGASIYPACQNLMLAARSLGLGTVLSTLHRLRKAQIHEVLRIPEGWESA
                     AIIPLGYPDRKYGPNKRRPVEEFIVYDRFPAPEAS"
     gene            complement(7054..7290)
                     /locus_tag="PLX85_00720"
     CDS             complement(7054..7290)
                     /locus_tag="PLX85_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HRC61728.1"
                     /translation="MTIDNLAFLPGSVTVTVGSTVVWRNGDQFPHDVTADGGGFGSNT
                     LQRGDTFSTTFSSAGTFTYMCTIHPSMHGTIVVN"
BASE COUNT         1233 a         2357 c         2609 g         1140 t
ORIGIN      
        1 gtgtggctcg acaagcggct ggatcgcctc gacggcatcg aacagcggcc cgggcttcat
       61 gaccatcccc gcgccgccgc cgaacgggta gtcatcgacc gtgcgatggc ggtccgtcgc
      121 ccagcctcgg aggtcgtgca agaccacctc aatcagcccg gcgtcgcgcg cacggcgaag
      181 gatcgactca tcgaggggcc cggcgaacat cccggggaac agcgtcagta cgtcgaagcg
      241 catgcgccca ttctggcgca ggatgcgcga gcgcgcctcg cgaggcgcta gcggccgcgg
      301 acggcgtcgt tcagcgcgtc gccgagcagg gcgaaggcga acatcagcga ggtcacgacc
      361 gccgcgggga cgagcaggag gtgcgggtgc gcctggaagg tgcgcgcccc cgagccctcg
      421 aagagcatcg cgccgaacga ggggttcggc ggctggacgc cgacgccaaa ccaggtcagc
      481 aggatctcgg agccggcgac ggagccgagc actgccgaga cctcgaggat gatcaggaac
      541 gagacgttcg gcatcaggtg acgccagatg atgtgccagg tgctgccacc cgtagcctgg
      601 gcggccagga tgaagtcgcg ctcccggagc tgcagcacct gcgcccgaat gagccttgcg
      661 gtgcccaccc aggagaacag cgcgagcgcg ccgaacactg tgaagtacga ggcggtgccg
      721 gagctggaga ggccgctgat cggggtgtga tcctcgaccc agccgaccca ctgcaggacc
      781 cgcggcagga gcgtggcatt gagcaggatc agcatcggta ggccgggtaa ggaggcgaag
      841 acgtcgccca cccgcatgat caggctgtca atccagccgc cgcggtagcc cgcgagcagc
      901 cccaggaagt tgccgaggat gagcgtgccg ctcagtacga cggccacggt cacgatgagc
      961 gtcgtccggg cggaatagag cacgcggctg agcatgtcgc ggcccagccg gtcagtgccg
     1021 agccagtggt ccgcgctcgg gccctcgaga ccgcgctcga ggttctgctc ggtgtaggag
     1081 tagggggcga ggagcggggc ggcgatgccg gcgccgtaga tgaccagcac gatcgcgagc
     1141 gacagcatcg cgaggcgctt cttgcgcagc cggccccaga gcaacagcca ggggttgcga
     1201 gcggcctggc gctcgtactg ctcgacggcc tcggcttcag ccgtgtactg accggccgcg
     1261 acgctcatac gaccgccgcc tcaccgagtc gcactcgcgg atcgatccac gagtacacga
     1321 tctcgaccag caggttcatg accacgaaca ggaaggcgac gatgaccgtc gtcgcgagga
     1381 tgacgtcgta gtcacggctc acgacggagt cgaacgtgag acggccgatg ccggggatgc
     1441 ccagcagcgt ctccgtgaag aaggcgccct cgacgatgcc gacgagggcg aagccaacaa
     1501 cagtgacgat cggcagcaac gagttcggca gcacgtgccg cacggccact cgagtctcag
     1561 gcaggccctt ggcgcgagcg gtgcggacgt agtcttcggt gtagacctcg agcgcggtga
     1621 cgcggacgag gcgggcgacg ccggcgaagc ccggcagcgt cagcgcgacc agcgggatgt
     1681 agaggtgcgg gtcggggatc gggacggcga tcacgccggc gatccaccag atgtcgaaaa
     1741 tgccgtccca acccccgacc ggcaggatgc tgttctgcac ggcgaagaac cagatcagcg
     1801 gcggaatcac gacgacgacc ggcagcgctc gcaggaatag gagcactgag atgatgaggg
     1861 ggtcgagcca cgtgccatgt aaccatgccg agagcagccc cagcgggatg ccgagcccaa
     1921 acacgacgaa cagcgctacg agcccgagct gcgccgacac ccacatcttc gggaagatga
     1981 tctcgcggac ggaatagccg cgctgtgcga aggactcccc aaagtcaccg cggacggcac
     2041 ggtccaccca cttcgcgtac tgctcgaaga tcgagccatc gaggccccgc tcggcgcgga
     2101 tgcgcgccag cacctcgggg tcgcggatct ggccggcggc gaccgagacc gggtcgccgg
     2161 ggccaaagcg cgcgatggag aacgtcagga acgagacggc cagcagcgtg accgggagcc
     2221 acaggagccg gcggatgatg tagccggcca tggccgcctc ctaccgttcc accgtgacga
     2281 atcggagccg cgggatgatc atcggcggtt cgatccaccc cttgatggcc ttgttcacga
     2341 cgacgtggtt ctgcgagaag tacaacggca gaatggcggc atccccgagg atcagcttct
     2401 cggcgtcctg gtacagcttg aggcgagccg cggggtcacg ctcgacgcgg gcctgctcga
     2461 gcagcttgtc gagctgtggg ttcgagtatt cgatgtcgtt gagcgaggac ttcgagtaga
     2521 acttcaggtc gaggatgttc tcagggtcgg gatagtccat gatccagccg ctgctgaaca
     2581 tctgcagctt gcccgcatcg acgtccgcga agaacgtcgc cgcgtccgac tgacggatct
     2641 ggacgtccac cccgaggacg ttcttccact gctcgatgaa tgcctgtgta tcgacgctgc
     2701 cctcggcgcc gccgccgatc tccgtcagcg tgatctgcgg catcttgccc gcgtacttcg
     2761 aggcggccag tgcgcgtttt gcctcgtcgg ggttgaacgg caacgtcttg tcgtccggcg
     2821 tgtagcccgg gagctgcggc tgcaggatgc ccgtagcggg cgtgagcatg ccggagaaag
     2881 tcacgtcggc aactcgcttg cggtcgatag cgaggccgaa ggcccgccgc acgttcgggt
     2941 catcgaatgg cggcgtcgtc gtgctgaacg cgatgtacgc gagcgagaac tccggggttc
     3001 gaacgtagag cttgttcaac gcgttgttct ggtcgcgcac acgatcgatg tcgttgaccg
     3061 agacgccggc gacatccagt tcgttgttct cgaagcgcgt gagctgcgag ccgccggcca
     3121 gctcatacac gacctgctgc accttcggcg cgccgaggtg gtagcgcgag ttcgcctcga
     3181 ggacgatgcg ctcgccgagg cgccactcac gcagcttgta ggggccggtg ccgttcggct
     3241 ggcgcgtcca gttgcgcggg ttcgactgag cctgctgaca gtcgatgacg aacgccgtgg
     3301 gataggtgag cttcgcgagg aagtacggct tcggctggtc aattgtgatg cgcaccgtgc
     3361 ggtcatcgat cacctcgatg ccgctgatgt ccttcgcggc gcccgccatg cgctccttcg
     3421 cgccgacgat gtcgttcagg taggccgcag cggtgctgga gccgagctcg cgcgaggcgg
     3481 cgcgctccat ggagcacttt acgtccgacg ccttcacctg gcgcccgttg tggaagaagg
     3541 cgttgtcgcg cagcttgaag gtatagacct tgccgtccgc gctgacatcg aaggattcgg
     3601 cgaggtcagg gacgatctcg aggttggggt tcagcgtcat gagcccgctg aatatctcga
     3661 cgatgtaggt cgccgacccg gcgtcgccgg cgatggcggg gtcaagcgag atgggatcgc
     3721 tgccgccgag ccgcagattg ccgctcgtcg ccgcggtcgt cgagccgccg ctcttcgagc
     3781 cgctgtcgtt ggacgaggag gcgtccttgc cgccgccgct caacaggagg atggccgaga
     3841 ggccaccgac gatcaggacc agccccgcca gcaccccgag cagaatggca aggatgcggt
     3901 tattaccgcc catcgatcgg ctccgcttct ctactgtgac gatctacgac ggtggggggc
     3961 cagcggttct gcgcatgata caggctcgcc cggaatcggc actcacgagc gcgcggcacg
     4021 cagatcgaga gcgatgtgca cgtcgcgcag ctggcgctcg ctgacgggcg agggcgcccc
     4081 ggtcatcggg tccgaggcgg acttcgtctt cgggaaggcg atgacctcgc ggatgtcgcg
     4141 ttcgccggcg aagagggcga ccgtgcggtc gatgccgggc gcgatgccgc cgtgcggcgg
     4201 agcgccgtac gtgaacgcct cgagcatgtg accgaacttc gtggtcgcct cctcctcggc
     4261 catcccgagg cgcgcgagga cgcgcatcag gagctcgcgg cggtggattc ggatcgagcc
     4321 agagccgatc tcctggccgt tgcagacgat gtcgtacgcg tgcgaacgca ccgcgcccgg
     4381 gtcggtgtcc atgagctcga cgtcctcggc gaacggcgca gtgaagaggt ggtggacggc
     4441 gctccagcgc tgctcgtcct cgtcccactc gaacatcggg aactgcgtga tgaaggcgaa
     4501 gatgagctga ccggggtcgg cgaggtcacg gtcctcggcc acgcggcgtc gcagcacgtc
     4561 gaggacacgc gaggtcacgg cgtcgcggtc ggcggcaatg agcagcaggt cgcctcgaga
     4621 cgccccgcag gcgcgaccga gggcggcgaa ctgctcaggc gtgaagaagc gtgcgacagg
     4681 cgagcggacg tcctcggcgg tcaggccgtc gagggagccc tcaccggtga aggcaaccgt
     4741 caccaggccc cccgcgccgt tctcgcgcgc cagctcggtc agtgcgtcga ggccacgccg
     4801 gggcgtgtcg gcgccgccgg gcacggcgat gcctcggatg cgccctcccg cctcggtggc
     4861 gttacggaag acggcgaact ccgtgtcggc cacgagcgag gtgaggtcgg cgatttccat
     4921 gccgtagcgg aggtcgggct tgtccgatcc aaagcgctcg atcgcctcgc ggtacgtcag
     4981 ccgcaggaac gggctcggta cctcgaactg cggaccgaca ttcgcggcca gctcggtgta
     5041 cagcccttcg atcagcgcga ggatgtcttc ctcctcgacg aatgacatct ccacgtcgag
     5101 ctgcgtgaac tcgggctggc ggtcggcgcg caggtcctcg tcgcggtagc agcgcgcgat
     5161 ctggaagtac ttctcgaagc ccgcgaccat cagcagctgc ttccactgct gcggtgactg
     5221 cggcagcgca tagaagctgc ccgcgtgcac ccggctcggc acgaggtagt cgcgcgcgcc
     5281 ctccggcgtc gcgaggttga ggacgggcgt ttctacttcg atgaagtcgc ggccgtcgag
     5341 gtagtcacgc atgaacttca cgacgcggtg gcggaggcgc aggttggcgg ccatccgctc
     5401 acggcgcagg tcgaggtagc ggtagcgcag gcgagtcagc tcgtcgacgg gggtgtcctg
     5461 gttgaccggg aagggcggcg tctccgcggg gttcagcacc tcgacgcgcg cagcgacgac
     5521 ctcgatggcg ccggtcggca ggtcggagtt ctcggtaccc gcgcgtcgtg gctgcacctc
     5581 gccctcgacg aggagcacga actcgttgcg cacctcggcg gcgacggccg aagcctcggg
     5641 cgcgatttgc gggttgaaga cgacctgcac gaggccggag gagtcgcgga ggtcgataaa
     5701 gatcaggccg ccgtgatcgc ggcggcggtg cacccaaccg gccagaatga ccggagtgcc
     5761 ggcgtcgcca gcccgcaact caccgcagcg atgcgtccgc atcacgtcgc gccactccct
     5821 cgtttgccct tgtttggatg cgagcgagtg tcgcacgggg ccagaggccg ggcaattcga
     5881 ggaagcctca gtctccggcg gcttcggcgc ggcgcgcttc ttcgatgcga ccgagcgcgc
     5941 gccccttgtc gggggtcggg tcctcgatga ggccgtagat ctcgcggccc tcgtgatcct
     6001 cgggaaggta ctcggtgatg gggaggccat cctcgttcac gaagaggagg cagtggcagt
     6061 acttgaactt ctgcatctcg tcgcaggcgc agatccaggt gcggcgcttc gcctcttcct
     6121 cgcgaggcag gtacaggccc tccggccagt tcccgtcctc gtccttcgtc gggtagaagt
     6181 tgcaggggca gatcgggcga ccgtactcat cgacgttgtg ggccaggccg aggacgaccg
     6241 cctccgtgat gctgcggtcg gggtgcaggc gggtgccgga cttctccgcg aacttctccg
     6301 cgtacttcca catcttgtcg atggactgct gcttcggtag ctccacgtac gacctcctgc
     6361 tccgcgatca gggtacccgg tctcgccccg gggttacgag gcctccggcg ccggaaagcg
     6421 gtcgtagacg atgaactcct ccacggggcg gcgcttgttc ggcccgtact tccggtcggg
     6481 gtagccgagc ggaatgatgg cggcgctctc ccagccctcg gggatgcgca gcacctcgtg
     6541 gatctgcgcc ttccgcagcc ggtgcagcgt actgagcacc gtgccaaggc ccagcgagcg
     6601 ggcggcgagc atcaggttct ggcaggccgg gtagatcgaa gcgcccgtct ggaagccggc
     6661 gcgcccgttt ccgggcacgc ggaccagcgg cacgatgagc acgggggcgt cggccagtgt
     6721 ctggcggtcg cgcgcgcggt cgggctcagc gccctgatac tgctcggcca tcgcctcctc
     6781 gtacagcgcg ctgacctgct ccttcacagc ctggtcgcgg atgacgagga accgccaggg
     6841 ctgcgagttc gagccggacg ggccatggat cgctgcctcg aggacctggc gcagtacgtc
     6901 gtcggaaacg ggccggtccg accaggcgcg caccgcgcgc tggtggcgga acagctgcat
     6961 gacgtcgtcg atcggctcca tctgtcaccc cctcggtagg ccgggctaca gactagccga
     7021 agcggcccac cgaggggact cctcgcgcga aggtcagttc acgacgatcg tgccgtgcat
     7081 cgacggatgg atggtgcaca tgtaggtgaa cgtgcccgcc gagctgaacg tggtgctgaa
     7141 ggtgtcaccg cgctgcagcg tgttggaacc gaacccgccg ccatcagcgg tcacgtcatg
     7201 cgggaactga tcgccgttcc gccacacaac agtgcttcca acagtgaccg tgacgctgcc
     7261 cgggaggaac gcgaggttgt cgatcgtcac gctcgcggtc gttgggggcg gcggcggggg
     7321 cgcctgggtg acgaccggc
//
LOCUS       DAORPG010000015         7029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_486_length_7029_cov_3.172211, whole genome shotgun sequence.
ACCESSION   DAORPG010000015 DAORPG010000000
VERSION     DAORPG010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7029
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_486_length_7029_cov_3.172211"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PLX85_00725"
     CDS             <1..598
                     /locus_tag="PLX85_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC61729.1"
                     /translation="GIVDQGYFFFQPAVYVSLEAWQAIAYQGDASQRPAASVVLLKGK
                     TLDASRGSGWEAVTKQTAFDNIEGVQAQQSTVNALRYMGLLIGAMVIGVFFYVITLQK
                     VSLLGILKAIGASGFYLVTQGLLQVAIVSAVGALLAVGLAVLLQATVLASSSVPIAFT
                     TSAVLTTALSVLVAGMIGAGLSVRQVAAIDPIIAMQQQ"
     gene            595..1314
                     /locus_tag="PLX85_00730"
     CDS             595..1314
                     /locus_tag="PLX85_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC61730.1"
                     /translation="MTDAILELRGVSKSFGEGDRRVDAVRDVTLDIDRGEMVSLVGPS
                     GSGKSTLLAMVGGLLQPTAGAIVVDGEDITRHGKGARTRYRRDRVGFVFQGSNMVPFL
                     TARENLLIVGEFGGVDRGALRRRADELLEELGLARVRDQVASQLSGGERQRVAIARAL
                     MRDPVLILVDEPTASLNSELGSRVVDALKEEIHGRSKACIMVTHDERMAERADRLIEF
                     RDGRLHSADARPETVRSSEAR"
     gene            complement(1311..2672)
                     /locus_tag="PLX85_00735"
     CDS             complement(1311..2672)
                     /locus_tag="PLX85_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HRC61731.1"
                     /translation="MARQYKYLLPEDQVPTRWYNIAADLKNPPAPALHPGTGQPLGPA
                     DLAPLFPMAVIEQEVSTERWIDIPEPVLDVYRTYRPAPLFRAHRLEEALGTPAHIYYK
                     YEGVSPAGSHKVNTAVAQAYYNHLEGVRRITTETGAGQWGTALAYACQMFGIELKIYM
                     VRVSFEQKPYRKAMMNVYGASVVPSPSQDTNAGRTILERDPNSSGSLGIAISEAVEDA
                     ATHEDTKYSLGSVLNHVMLHQTVIGQEAIAQMQLAGEYPDVVIGCVGGGSNYAGLAFP
                     FMQERLNGGRNTRFVAAEPASCPTLTKGVYTYDFGDTAELTPLVKMSTLGHSFMPPSI
                     HAGGLRYHGMSPLISQLHEEGYIDAVAHPQLGVFAAAVQFARAEGIVPAPESAHAIRS
                     AIDEALEAKEAGEERVILFNLSGHGFLDLGAYEQYFNGELEDYEYPAELVQQALAGVP
                     QLA"
     gene            complement(2788..4020)
                     /locus_tag="PLX85_00740"
     CDS             complement(2788..4020)
                     /locus_tag="PLX85_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRC61732.1"
                     /translation="MTDVVASHRDELVFMLNQATELEHSLSCSYLFTAFSLKKSVEEG
                     LPADAVESVLGWKRSIIQVAVEEMFHLAVVNNLLTALGAAPNFDRPNFPHDCAWYLPD
                     YQIELRPFSEEVLRHFIAVEQPDGSNLPAYTSPERLQRIEGDMDNKIGADPRQFDSQG
                     DVYSVAENSLRALVERLGEENVFVGPPPRPAFRRFFESSGWEPIVDIGTTARNLAHVV
                     EQGEGDSSGNPDSHYARFNRILEEYLAFCETHPGFEPAYPMLENPFTRTPPEANGPVN
                     LIDHEPAIRLSDLFNEAYVSMLHLLARFFVMTDESDEEADALCNTAIETMAGTLVPLG
                     ELLARMPAGVRHPGRTAGPSFVVRTLHPLPYKDAAWRLLRERFGELADHADELSTAGL
                     PELEQVAKTFRGVVRQLS"
     gene            4214..4882
                     /locus_tag="PLX85_00745"
     CDS             4214..4882
                     /locus_tag="PLX85_00745"
                     /inference="COORDINATES: protein motif:HMM:NF020917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HRC61733.1"
                     /translation="MDDQPHIQIDVNGPYIVDGPVPLTEMAPVHTLNGEPVDWHVLRA
                     IESEEWPAYLCRCGGSKNKPFCDSSHESNGFDGTEAADRRPFAERAVVDEGAHEQLLD
                     DKPLCVRAGFCRTRTTSVWDLLALGGDPEAAGRLREMTWNCPSGRLVLRDLDGNDIEP
                     ELAPEVGVVPGGPLWIRGGIRVTGSDGTEWETRNRVALCRCGGSASKPFCDGAHLRRH
                     FDER"
     gene            complement(4914..5678)
                     /gene="heR"
                     /locus_tag="PLX85_00750"
     CDS             complement(4914..5678)
                     /gene="heR"
                     /locus_tag="PLX85_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HRC61734.1"
                     /translation="MTDETTTRRFRRLRWYNLGMGVLHLAQGLALLALSNDFSLPVHA
                     TFMQGPPGSGPAELDHLFDLRIGPAVAAFVFISAAAHFLLVLPGVFGWYTRNLERRRN
                     DARWIEYAFSASIMIVLIAMLTGIGDIAALVGLFGVNATMILLGLVQEHTERPGDGGL
                     LPFWVGCIAGAVPWVAIAIYFVTPGSPADPPAFVYGIFISLFLFFNVFALNMWLQYRR
                     VGPWRDYVFGETVYVFLSLTAKSALAWQVFASTLAD"
     gene            5791..6333
                     /locus_tag="PLX85_00755"
     CDS             5791..6333
                     /locus_tag="PLX85_00755"
                     /inference="COORDINATES: protein motif:HMM:NF040769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HRC61735.1"
                     /translation="MTTETARLNVLTPEGGSLSLETLWADAPLLLVLVRHYGCQFCRE
                     QIAQLRRILPDLERAGVDVAIIGNGTPLMAQAFVEETGLEVPLYTNPGREVYRALGAR
                     RPSLLAMLDPRLWLNGLRAMSRGYLPHRVQGDAAQLGGVFLILPDASMPFAHRSDRGG
                     DYPSNASILDAVRSLERMAA"
     gene            complement(6340..6699)
                     /locus_tag="PLX85_00760"
     CDS             complement(6340..6699)
                     /locus_tag="PLX85_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HRC61736.1"
                     /translation="MPDANSRLVYSTDGTDGGRVRPPVTPRGSSQAAPRPADPRDGYV
                     RVRREKKGRGGKTVTVVSGIEGTAADLDRWLKLLKAHCGAGGTRDGNVLVIQGDHRDR
                     VQARLEEGGLRVKQAGG"
     gene            6884..>7029
                     /locus_tag="PLX85_00765"
     CDS             6884..>7029
                     /locus_tag="PLX85_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HRC61737.1"
                     /translation="MSVIAWIVLGLIAGALAKMLMPGRDPGGILGTILIGIGGAIVGG
                     FIWNA"
BASE COUNT         1130 a         2325 c         2386 g         1188 t
ORIGIN      
        1 cggcatcgtg gatcaggggt acttcttctt ccagccggcg gtgtacgtct ccctcgaggc
       61 gtggcaggcg atcgcgtacc agggcgacgc gagtcagcga cccgccgcca gcgtcgtgct
      121 gctcaagggg aagacgctcg atgcgtctcg cggaagcggc tgggaggccg tcacgaagca
      181 gaccgcgttc gacaacatcg agggtgtcca ggcacagcag tcgaccgtga atgcgctccg
      241 ctacatggga ctgctgatcg gcgcgatggt gatcggcgtc ttcttctacg tcatcacgct
      301 ccagaaggtg tcgctgctcg gcatcctcaa agcgatcgga gcgtccggct tctaccttgt
      361 gacgcagggg ctgctgcagg tcgccatcgt ttcggccgtt ggcgcactgc tggccgtggg
      421 gctggcggta ctgcttcaag ccacggtcct cgcctcgagc agtgtgccga tcgccttcac
      481 gaccagtgcc gttctgacga ccgcgctctc ggtgctggtc gcgggcatga ttggcgccgg
      541 cctctccgtg cgacaggtcg ccgccatcga tccgatcatc gcgatgcagc agcaatgacg
      601 gacgccatcc tcgaactgcg aggcgtctcc aagtcgtttg gcgaaggaga ccgccgcgtc
      661 gacgccgtcc gagatgtcac gctcgatatc gatcgtggcg agatggtgtc gctggttgga
      721 ccgtcgggtt ccgggaagtc gaccctcctc gcgatggtgg gaggcctgct gcagcccacc
      781 gcgggcgcca tcgtcgtgga cggcgaggac atcactcgac acggcaaggg ggcgcgtacg
      841 cgctaccggc gcgatcgggt gggcttcgtg ttccagggca gcaacatggt gcccttcctg
      901 accgctcgcg agaacctgct cattgtcggc gagttcggcg gcgtcgaccg cggcgcgctg
      961 cgtcgtcgcg cggacgagct gctcgaggag ctcgggctcg cccgcgtgcg tgaccaggtc
     1021 gcatcacagc tctccggtgg cgagcgtcag cgggtagcga tcgcgcgcgc cctgatgcgt
     1081 gaccccgtgc tcatcctcgt ggacgagccg accgcgagcc tgaacagcga actgggttcg
     1141 agggtggtgg acgcgctgaa ggaagagatc catggccgca gcaaggcctg catcatggtg
     1201 acccacgacg aacgcatggc cgagcgtgct gaccgcctga tcgagtttcg agacgggcgg
     1261 ctgcactcgg ccgacgcccg ccccgaaacc gtgcgctcgt ctgaagctcg ttaggcgagc
     1321 tggggcactc ccgcgagtgc ctgttgcacc agctccgcgg ggtactcgta gtcctcgagc
     1381 tccccgttga agtactgctc gtaggcgccg aggtcgagga acccgtggcc ggagaggttg
     1441 aacaggatca cccgttcctc gcccgcttcc ttcgcctcca gcgcctcgtc gatcgccgag
     1501 cggattgcat gcgcggattc cggcgccggg acaatgccct cggcgcgtgc gaactgcacg
     1561 gctgcggcga acaccccgag ctgcggatgc gctacggcgt cgatgtagcc ttcctcatgc
     1621 agctggctaa tcagtggcga catgccgtgg tagcgcaggc cgcccgcgtg gatcgagggc
     1681 ggcatgaagc tgtgcccgag cgtcgacatc ttcacgagcg gtgtcagctc cgccgtgtcg
     1741 ccaaagtcgt aggtgtacac gcccttcgtg agcgtgggac acgaggcagg ctcagccgcc
     1801 acgaaccggg tgttgcggcc gccgttgagc cgttcctgca tgaacgggaa cgcgagaccg
     1861 gcgtaattcg agccgccgcc gacgcacccg atgaccacat ccgggtactc cccagccagc
     1921 tgcatctgcg cgatcgcctc ctggccgatc accgtctggt gcagcatgac gtggtttagc
     1981 acagacccga gcgagtactt cgtgtcctcg tgggtcgccg cgtcctcgac cgcctccgag
     2041 atcgcaatgc cgagcgatcc gctcgagttc ggatcgcgct cgagaatagt gcggccggcg
     2101 ttcgtgtcct ggctcgggct cggcacgacc gaggcgccgt agacgttcat catcgccttg
     2161 cggtacggct tctgctcgaa cgagacgcgc accatgtaga tcttcagttc gatgccgaac
     2221 atctgacacg cgtacgcgag cgccgtcccc cactgaccgg ccccggtctc ggtcgtgatg
     2281 cggcggacgc cctcgaggtg gttgtagtag gcctgtgcga cggcagtgtt caccttgtgc
     2341 gagccggccg ggctcacgcc ttcgtacttg tagtagatgt gcgccggcgt ccccagtgcc
     2401 tcctcgaggc gatgggcgcg aaacaggggc gccggacgat aggttcgata cacgtcgagc
     2461 accggctccg gaatgtcgat ccagcgctcc gtggagactt cctgctcgat gaccgccatc
     2521 gggaacagcg gagcgaggtc ggcaggcccg agcggctgac cggtgcccgg gtgcagcgcg
     2581 ggagcgggcg ggttcttcag gtcggccgcg atgttgtacc accgcgtcgg cacctgatcc
     2641 tcgggcagga ggtacttgta ctgtcgggcc acggatccac tccctttcgc acgaccgggt
     2701 ggcgagcgcc agcgccccgg ttcagtagac gcgcccggca gtatagtccc cgagcgatcg
     2761 agggagcagg gccgttcggc cctcgcctca ggagagctgc cgcaccacgc ctcggaaggt
     2821 cttagccacc tgctcgagct ccgggagtcc ggcggtggac agctcgtcgg cgtggtccgc
     2881 gagctcgccg aagcgctccc ggagcagccg ccaggcggcg tctttgtagg gcagcgggtg
     2941 cagtgtccgc acgacgaagc tcggtccggc ggtacgaccc gggtggcgca cccccgcagg
     3001 catgcgcgcg agcagctcac cgagcggcac gagcgtgccc gccattgtct cgatcgccgt
     3061 gttgcacagc gcgtcggcct cctcgtcgct ctcgtccgtc atcacgaaga agcgtgcgag
     3121 cagatggagc atcgagacgt acgcctcgtt gaacaggtcg ctgagccgaa tcgccggctc
     3181 gtgatcgatc aggttgactg gaccgttcgc ttccggaggc gtgcgcgtga acgggttctc
     3241 gagcatgggg tatgccggct cgaagcccgg gtgcgtctcg cagaaggcga gatactcctc
     3301 gaggatgcga ttgaagcgag cgtagtgaga gtcgggattc ccgctcgagt cgccctcacc
     3361 ttgctcgacg acgtgcgcga ggttgcgcgc ggtggtcccg atgtcgacga tcggttccca
     3421 cccgctggac tcgaagaagc gccggaatgc cggccgcggg ggcgggccca cgaagacgtt
     3481 ttcttcgccc agccgctcga caagcgcgcg cagactgttc tcggccactg agtacacgtc
     3541 gccctgggag tcgaactggc gggggtcggc cccgatcttg ttgtccatat cgccctcgat
     3601 gcgctggagc cgctcggggg aggtgtacgc ggggaggttc gagccgtccg gctgctcgac
     3661 cgcgatgaag tggcgcagca cctcctcgct gaacggtcgt agctcgatct ggtagtcagg
     3721 caggtaccac gcgcagtcgt gagggaagtt cgggcggtcg aagttcggag ctgctcccag
     3781 ggcggtcagc aggttgttga ccaccgcgag gtggaacatc tcctcgacgg ccacctggat
     3841 gatgctgcgt ttccagccga gcaccgactc caccgcgtcg gcgggcagtc cttcctcgac
     3901 gctcttcttc agggagaagg ccgtgaacag gtagctgcac gacaggctgt gttccagctc
     3961 ggtcgcctgg ttcagcatga agacgagttc atctcggtgg gatgcgacga cgtcggtcac
     4021 gagcgctctc cgtttcgact ggaaggacgc aacgcaccac gctcgcgcgg actgcgctgc
     4081 cgaggcgcga acggacggta ctccggcgac ccatcgcgag caacgccgct ccaggaaaac
     4141 cgcgagataa gcatgtttcg ccggcgatca ctatgctgtg cgaccgaaca acgacgctcc
     4201 gagggagaac aaggtggacg accagccgca catccagatc gacgtcaacg gcccctacat
     4261 cgtggatggc cctgtgcccc tcacggagat ggcgccagtg cacacgctga atggcgagcc
     4321 cgtcgactgg catgtcctcc gtgccatcga gtccgaggag tggccggcct atctgtgccg
     4381 ctgcggaggc tcgaagaaca agccgttctg cgacagcagc catgagtcga atggcttcga
     4441 cggaaccgag gcagcggatc gccgcccgtt cgcggagcgc gccgttgtgg acgagggcgc
     4501 gcacgagcaa ctgcttgacg acaagccgct ctgcgtgcgg gccggcttct gccgcacgcg
     4561 cacgaccagt gtctgggatc tgctggcgct cggtggcgac cccgaggccg ccggacggct
     4621 ccgcgagatg acctggaact gcccgtcggg ccgactcgtc ctgcgagacc tcgatggcaa
     4681 tgacatcgag cccgagctcg cgcccgaggt cggggtggtc cccggcggtc cgctctggat
     4741 tcgcgggggc atccgtgtga ctggctcaga tggcacggaa tgggagacga ggaaccgcgt
     4801 ggccctgtgt cgctgcggtg gctcggccag caagccgttc tgcgacgggg cccacctgcg
     4861 gcgtcatttc gacgagcggt agcacgccgc aaacggctgc tcggcgccgc gggtcagtcc
     4921 gcgagcgtcg aggcaaagac ctgccacgcg agcgcggact tcgccgtcag gctgaggaag
     4981 acgtataccg tctcgccaaa gacatagtcg cgccacggcc cgacccgccg gtactgcaac
     5041 cacatgttca gcgcgaagac gttgaagaac agaaacagcg agataaagat cccgtagacg
     5101 aacgcgggtg gatccgctgg cgaaccgggc gtcacgaagt agatcgcaat cgcgacccac
     5161 ggcacggcgc ccgcgatgca gcccacccag aacgggagca acccgccgtc gcccggccgc
     5221 tccgtgtgct cctgcaccag gccgagcagg atcatcgtcg cgttcacccc gaacaacccg
     5281 acgagcgcgg cgatgtcgcc gatgcccgtg agcatcgcaa tcagcacgat catgatcgag
     5341 gcgctgaacg cgtactcgat ccagcgcgcg tcgttgcggc ggcgttcgag gttcctcgtg
     5401 taccacccga acacgcctgg caacacgagg aggaagtgcg ccgccgccga gatgaatacg
     5461 aacgccgcga ctgccggccc gatgcgcaga tcgaagagat ggtcgagttc agctggcccc
     5521 gaacccggtg ggccctgcat gaacgtcgcg tgcacgggta gcgagaagtc gttgctcagc
     5581 gcgaggagcg ctaggccctg cgccaggtgg agcacgccca tcccgaggtt gtaccagcgg
     5641 agccgccgga atcgccgcgt cgttgtctcg tcggtcacgc atcctcccgc catctcgtgc
     5701 gtctgaatcc agcgtagcgc atcgccggag cgccgcccgc ggcccccgta cactggatcg
     5761 tgaagagaac ggagcgcgca gttggagcga gtgacaaccg agacggcaag gctcaatgtc
     5821 cttacgcccg agggtggctc gctttccctc gagacgctgt gggcggatgc gccgctactc
     5881 ctggttctcg tgcgccacta tggctgccag ttctgccgcg agcagatcgc gcagttgcgt
     5941 cgtatcctgc ccgacctgga gcgggccggg gtggacgtcg ccatcatcgg caacggtacg
     6001 ccgctcatgg cgcaggcatt cgtcgaggag acgggtctcg aggttccgct ctatacgaac
     6061 ccgggccgtg aggtctaccg tgcactcggc gcgcggcgtc cctcgctgct cgccatgctc
     6121 gacccgcggt tgtggctgaa cggcctgcga gcgatgtcgc gcggctacct cccccaccgc
     6181 gtccagggcg acgccgcgca gctcggtggc gtgttcttga tcctgcccga tgcctcgatg
     6241 ccgttcgccc atcggagtga ccgcggaggc gactacccgt cgaacgcctc tatccttgac
     6301 gccgtccgtt cccttgagcg gatggcggcc tgacgcgcgt cagccgccgg cctgtttgac
     6361 acgcagaccg ccctcttcga gccgggcctg cacgcggtcg cgatggtccc cctggatgac
     6421 caggacgttg ccgtcgcgcg tccctccggc gccgcagtgc gccttcagca gcttcagcca
     6481 acggtcgagg tcggcggccg tcccctcgat gcccgagacc acagtcacgg tcttcccgcc
     6541 gcggcccttc ttttcgcgcc gcactcgcac gtatccgtcg cgcgggtccg ccgggcgcgg
     6601 cgccgcctgc gacgaacctc gaggcgtgac gggtggccgc accctcccac cgtcggtgcc
     6661 atcggtggag tagacgaggc gcgagtttgc gtcgggcatc gaggctccaa tcgcgtgctt
     6721 cctcagggcc tccattgtcg cccgcaaggc ccgtgcctgc ctgtcacaaa tcgagatgga
     6781 ggcgcgacgt tcgcccgcac atgttcgatg tcgtcgtccg ccgtcctcga tcacgatgta
     6841 cacgggagag acggtgacgg cgggcgtggg aaggggtcac acggtgagtg tcatagcctg
     6901 gatcgtgctc ggcctgattg cgggtgcgct ggcgaagatg ctgatgccgg gacgcgaccc
     6961 cgggggcatc cttggcacca tcctgattgg catcggcggg gcaatcgtcg gcggcttcat
     7021 ctggaatgc
//
LOCUS       DAORPG010000016         6975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_495_length_6975_cov_3.340896, whole genome shotgun sequence.
ACCESSION   DAORPG010000016 DAORPG010000000
VERSION     DAORPG010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6975
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_495_length_6975_cov_3.340896"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..411)
                     /locus_tag="PLX85_00770"
     CDS             complement(97..411)
                     /locus_tag="PLX85_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRC61738.1"
                     /translation="MEQSSQWTFLTNHAHVLICIARDPDVRLRDVADAVGITERAAQR
                     IVQELETAGYLEHERVGRRNSYRLNPQLPLRHPLDREHRLAELLEAIGTRAGQPRESV
                     PV"
     gene            599..1042
                     /locus_tag="PLX85_00775"
     CDS             599..1042
                     /locus_tag="PLX85_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRC61739.1"
                     /translation="MHIGTKGDYGVRALIDLAMYGGEGPVQRTEIARRQHIPDSYLDH
                     LLAQLRRDGFVRSTRGPGGGHQLTRPADEICLLHVLESLEGSLAPLPCLEDDESGEPH
                     SVCAQQWVWREIYDDMRARLAAVSLADLAAREQERLRSAVASYSI"
     gene            1066..1998
                     /gene="cysK"
                     /locus_tag="PLX85_00780"
     CDS             1066..1998
                     /gene="cysK"
                     /locus_tag="PLX85_00780"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRC61740.1"
                     /translation="MPETRSPSTPLIADSVLDLIGATPLVRLHRVAPAGVEILGKLEA
                     RNPAGSVKDRIALSMVEDAERAGRIQPGDTLVEPTSGNTGIGLAMVCARKGYHLILTM
                     PEDMSIERRRLLERFGAELILTPAIEGMTGAVFTAQELARERGYFMPQQFDNPANPEV
                     HRRTTGPEIIEATEGRLDAFVAGVGTGGTITGVGEVLRNARIPARIVAVEPARAPVLQ
                     GGRAGLHGIQGIGASFVPGVLNREVYDEIIGVRDEDAYAMTKRLAREEGLLVGISSGA
                     NVWAAIQVAEAFGPGKRVVTMLCDTGERYLSVAL"
     gene            2034..3191
                     /gene="moeB"
                     /locus_tag="PLX85_00785"
     CDS             2034..3191
                     /gene="moeB"
                     /locus_tag="PLX85_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HRC61741.1"
                     /translation="MAVSVYIPSPFRRLTQNHEYVSVEGRNVAEVLDAVDERYPGFGN
                     LVYDRERTIPAHINIYVNNQEIHDLSGTDTTLEDGDQVAVIPALAGGADDHANGNGAA
                     PAGNPAALSPEEVMRYSRHIIMGQVGPTGQRKLRNAKVLIVGAGGLGSPVAIYLALAG
                     VGTVGIVEFDTVDLSNLQRQLLHQTADVGKTKMQSAVETLSAYNPHVTVRQHEAPITS
                     DNAFEIIRDYDYVVNGADNFATRYLVNDASYLLGKTLIDGSILLFDGQATVFKPGQGC
                     YRCLYPTPPPPGLVPSCAEAGVLGAITGIIGSIQATEVFKQILDIGQPLVGRLLLVDA
                     LTMEFRTMKLRRDPDCPLCGDNPTVTELIDYDQFCGSPPLVAPGATGAAHH"
     gene            3287..3556
                     /locus_tag="PLX85_00790"
     CDS             3287..3556
                     /locus_tag="PLX85_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006371568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD family protein"
                     /protein_id="HRC61742.1"
                     /translation="MQVHVTSVIQKAVDGQREFAADGATVSELIENIESRYPGFRSQI
                     MDASGQLHRFVNIYLNDEDIRYLGGADTTLKDGDQVSILPALAGG"
     gene            3569..4504
                     /locus_tag="PLX85_00795"
     CDS             3569..4504
                     /locus_tag="PLX85_00795"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HRC61743.1"
                     /translation="MLYRSMLDMVGNTPLVEVAVFSPKPSVHIYVKLEGQNPTGSVKD
                     RIAKYMVESAEREGRLKPGQTILEPTSGNTGIALAMIGQLRGYPVKVVMPDAVSPERV
                     ELLRAFGAEIIYSPGDLGTNGSVEMARQVHAEHPDWFMPFQYENRDNPRAHYETTAPE
                     IIADCPDIDVFVAGLGTGGTLTGTGRRLKEHNPKVRVVAAAPHPGDLVQGLRSLEEGY
                     IPPVLDESILDGRIVVDSRTSFRMTRELTAKAGVFAGISSGAAVRAAQKVAERMDEGH
                     IVCLLADGGWKYLSSGLWTQDYDNIEEDVGTKLWW"
     gene            4560..5384
                     /locus_tag="PLX85_00800"
     CDS             4560..5384
                     /locus_tag="PLX85_00800"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006446720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HRC61744.1"
                     /translation="MAEKLVTVDWVAEHRNDPNVRLVEVDVDTEAYGTGHIEGAVGWN
                     WTTQLQDQVRRDIISKPDLEQLLGASGIGNDTHVVLYGDNNNWFAAYALWLLELYGHE
                     NASLMDGGRLKWMADNRPVTNDVPSYPAASYSAKDANPALRAQRDEVLQAVGGAASQL
                     VDVRSPAEYNGEIIAPPGMTETAQRAGHIPGAKNIPWARAVNEDGTFKSPDDLKALYG
                     GAGVEEGKPTIAYCRIGERSSHTWFVLKHILGYGDVKNYDGSWTEYGSLIGVPVEK"
     gene            5408..5791
                     /locus_tag="PLX85_00805"
     CDS             5408..5791
                     /locus_tag="PLX85_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61745.1"
                     /translation="MDEEQVAAAALQRALLNRALVSNDAGRLLLRFHASVMDRYREQG
                     ATLIRTRTVGRVSKPGRWSLDVGIAAGGTEVQVPFDDLLERLPEAERPHWVEHLVVEP
                     SSLNFLRMRLAGAACIDDGEAEPWT"
     gene            5811..6281
                     /locus_tag="PLX85_00810"
     CDS             5811..6281
                     /locus_tag="PLX85_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61746.1"
                     /translation="MKPGAIIVIGLGLFIIFGLLAAGLSLSTTKSPFAVTTQVIEAMP
                     AEPPADGSASVVYATSRTSSGLKILGVQVRQPDFAASVGFVPPPGCAPPQGVVLEDEG
                     LCSGVALTGVVMGSGTTRSGHSLVIVQVAISSACHDVLREGDPWPSTHTACQEP"
     gene            6369..6809
                     /locus_tag="PLX85_00815"
     CDS             6369..6809
                     /locus_tag="PLX85_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HRC61747.1"
                     /translation="MPRIPRALIDEMVAHAREDLPNEACGMVHAREGAPVAVHRVRNT
                     TASPYRYEMDGLEVARLERQRDESGETLFAIYHSHVASEARPSMTDIRQAFFPPGEIE
                     TGLFMYPEALYILVSLADDPPPSVRAWRIVREEPHLIEEPIEVV"
     gene            6820..>6975
                     /locus_tag="PLX85_00820"
     CDS             6820..>6975
                     /locus_tag="PLX85_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRC61748.1"
                     /translation="MPLDAAPCPAPIRIGNRTFEWGARTYIMGILNVTPDSFSGDGVL
                     DPSRAAER"
BASE COUNT         1142 a         2582 c         2159 g         1092 t
ORIGIN      
        1 gttgtgggtg ttgacgatca tgtgcagcaa agcggaagtc ctttcttcgc ttctcgactg
       61 agtggctcgt cccgctggaa cggcccggtc gcatggtcag acggggacgg actcgcgcgg
      121 ttgtccggct cgcgtaccga tcgcctcgag cagctccgca aggcggtgtt cgcggtccag
      181 cgggtgacgg agggggagct ggggattgag ccggtaggag tttcgcctcc ctacccgttc
      241 atgctcgaga tacccggcgg tctccagctc ctgcacgatt ctctgggcgg cgcgctcggt
      301 gatcccgacc gcgtcagcga cgtctctgag ccggacgtcc gggtcgcgag cgatgcagat
      361 caggacgtgc gcgtggttcg tcaggaacgt ccattgcgat gattgctcca tgctgaggtc
      421 tccgaatgcc gccggtcgtc cgcggtgggg cttgcaggcg atatacgaat cataaatcgt
      481 gcaatatcgt gcgtgcaaga cgcgcaatca cgatccgaat gcggcagagc cctgccccgc
      541 ctctctcgtc acgtgctatg atcctgacta acttagtcgt gaattcgagg cgtggacgat
      601 gcacatcggc acgaagggcg attacggcgt acgtgcgctg atcgacctcg ccatgtatgg
      661 cggggagggc cccgtgcagc gcacggagat cgcccggcgc cagcacatcc ccgactccta
      721 cctcgaccac ctgctggcgc agctccgccg tgacggcttc gtacgcagta cgcgcggccc
      781 aggcggcggc caccagctca cccgccccgc cgatgagatc tgcctgctgc acgtcctcga
      841 gagcctcgaa ggatccctgg cgcccctgcc ctgcctcgag gacgacgagt caggcgagcc
      901 gcactccgtc tgtgcccaac agtgggtgtg gcgcgagatt tacgacgaca tgcgcgcccg
      961 cctcgcggcg gtgtcgctgg ctgacctcgc cgcgcgcgag caggagcgcc tccgctccgc
     1021 cgtcgcgagc tattcgatct agaagcggga agtgacggaa tgacgatgcc ggagacccgg
     1081 tcgccctcga ctccgctgat cgcggactcg gtgctcgatc tgatcggggc gacgccactc
     1141 gtccgactcc atcgcgtcgc ccccgccggc gtcgagatcc tcggcaagct cgaagcccgc
     1201 aacccggcgg gctccgtgaa ggaccgcatc gccctctcga tggttgagga cgctgagcgg
     1261 gctggtcgga ttcagcctgg cgacacgctc gtcgaaccta cgtccggcaa cactggcatc
     1321 ggcctcgcca tggtctgcgc acgcaagggc taccacctca tcctcacgat gcctgaggac
     1381 atgagcatcg aacgccgccg cctcctcgag cgcttcggcg cggagctcat cctcaccccc
     1441 gccatcgagg gcatgaccgg cgccgtcttc accgcgcagg aactcgcccg cgagcgcggc
     1501 tacttcatgc cgcagcagtt cgacaacccc gcgaaccctg aggtccaccg ccgcacgacg
     1561 ggccccgaga tcatcgaggc caccgagggc cggcttgacg ccttcgtcgc cggcgtcggc
     1621 actggcggca cgatcactgg cgtgggcgag gtgctgcgga acgctcgtat tccggcacgc
     1681 atcgtcgccg tcgagcccgc ccgcgccccg gtcctccagg gcggacgcgc cgggctccac
     1741 ggtatccagg gcatcggcgc ctcgttcgtc cccggcgtgt tgaaccgcga ggtctacgac
     1801 gagatcatcg gtgtacgcga cgaggacgcc tacgcgatga cgaagcgtct ggcgcgcgag
     1861 gagggcctgc tcgtcggcat ctcctccggc gcgaacgtct gggcggcgat tcaggtcgct
     1921 gaggccttcg gccccggcaa gcgtgtcgtc accatgttgt gtgataccgg cgagcgctac
     1981 ctcagcgtgg cgctgtaacg tatcgaagca ttcgagagcg aaagggactc cctatggccg
     2041 tcagcgtcta catcccctcg ccgttccggc gtctgacgca gaaccacgaa tacgtctccg
     2101 tcgagggacg caatgtcgcc gaggtgctcg acgccgtcga cgagcgctac cccggcttcg
     2161 gtaacctcgt ctacgaccgc gagcgcacca tcccggcgca catcaacatc tacgtgaaca
     2221 accaggagat tcacgacctc tccggcaccg acaccaccct cgaggacggc gaccaggttg
     2281 cggtcatccc cgccctcgcc ggcggcgcgg acgaccacgc gaacggcaac ggcgccgccc
     2341 ccgcgggcaa ccccgccgcc ctctcgcccg aggaagtcat gcgctactcg cggcacatca
     2401 tcatgggcca ggtcggtccc accgggcagc gcaagctccg caacgcgaag gtcctgatcg
     2461 tcggcgcggg cggcctcggg tcccccgtcg cgatctacct cgcgcttgcc ggcgtcggca
     2521 ccgtcggtat cgtcgagttc gacacggtcg acctgtcgaa cctccagcgc cagttgctcc
     2581 accagaccgc ggacgtcggc aagacgaaga tgcagtccgc ggtcgagacg ctgagcgcct
     2641 acaacccgca cgtcaccgtc cgccagcacg aggcgccgat tacctcggac aatgccttcg
     2701 agatcattcg cgactacgac tacgtggtga acggcgcgga taacttcgcc acccgctacc
     2761 tcgtgaacga cgcgagctac ctgctgggca agaccctcat cgatggttcc atcctgctct
     2821 tcgatggtca ggccaccgtc ttcaagcccg gccagggctg ctatcgctgc ctctacccca
     2881 cgccgccccc gcccggcctc gtgccgtcgt gcgccgaggc cggcgtcctc ggcgcgatca
     2941 ctggcatcat tggctccatc caggccaccg aggtctttaa gcagatcctt gacatcggcc
     3001 agccgctcgt cggccgcctg ctcctcgtgg acgcgttgac gatggagttc cgcacgatga
     3061 agctgcgtcg tgacccggac tgcccgctct gcggcgacaa cccgaccgtc accgagctga
     3121 tcgactacga ccagttctgc ggctcgcccc cactcgtcgc gcccggcgcg acgggggctg
     3181 cccaccactg agcaacacca tccgaggcgc cccgcagcgg gcgcctcggt tacgccgcct
     3241 cgcgcggcgt cagaccggac ctgaggaaaa gggggccacc ttgtcggtgc aggtgcacgt
     3301 cacgtcagtg attcagaagg ccgtcgatgg ccagcgcgag ttcgccgccg acggtgccac
     3361 cgtgagcgag ctcatcgaga acatcgagag ccgctacccc ggcttccgct cccagatcat
     3421 ggatgcgtcc ggccagctgc accgcttcgt caacatctac ttgaacgacg aggacatccg
     3481 ttacctcggc ggcgcggaca ccacgctcaa ggacggcgac caggtctcca tcctgcccgc
     3541 cctcgccggc ggctagggac tcgccccgat gctctatcgc agcatgctcg acatggtcgg
     3601 caacacgccg ctcgtcgagg ttgctgtctt ctcccccaag ccctccgtcc acatctacgt
     3661 gaagctcgag ggccagaacc ccaccggctc cgtcaaggac cgcatcgcca agtacatggt
     3721 cgagtccgcc gagcgcgagg gccgcctcaa gcccgggcag accatcctcg aaccgacctc
     3781 cggcaatacc ggcatcgccc tggcgatgat cggccagctc cgaggctacc cggtgaaggt
     3841 cgtcatgccc gacgccgtca gtccggaacg cgtcgaactc ctgcgcgcct tcggcgcgga
     3901 gatcatctac tcgcccggcg acctcggcac gaacggctcg gtcgagatgg cccgccaggt
     3961 ccatgcagag caccctgact ggttcatgcc gttccagtac gagaaccgcg acaacccgcg
     4021 cgcccactac gagacgaccg cccccgagat catcgccgac tgcccggaca tcgatgtctt
     4081 cgtcgccggc ctcggcacgg gcggcacgct gacgggcacc gggcggcgcc tcaaggagca
     4141 caacccgaag gtccgcgttg tagccgccgc cccgcacccg ggcgacctcg tccagggcct
     4201 gcgcagtctc gaggagggct acatcccgcc cgtcctcgac gagtccatcc tcgacggccg
     4261 catcgtcgtc gactcccgca cgtcgttccg catgactcgc gagttgacgg cgaaggccgg
     4321 cgtcttcgcc ggcatctcga gcggcgctgc cgtccgcgcc gcgcagaagg tcgccgagcg
     4381 gatggacgag ggccacatcg tctgcctcct cgcggacggc gggtggaagt acctttcctc
     4441 gggcctctgg acgcaggact acgacaacat cgaggaggac gtcggcacca agctctggtg
     4501 gtagcgcggc tctgcgccgc gacattgtgc ctgtctatca cgcaatcggg aggggacaca
     4561 tggcagagaa actcgtgacc gttgactggg tcgccgagca ccgcaacgac ccgaacgtcc
     4621 gtctcgtcga ggtcgacgtc gacaccgagg cgtacggcac cggccacatc gagggcgccg
     4681 tcggctggaa ctggaccacc cagctccagg accaggtgcg ccgcgacatc atctcgaagc
     4741 ctgacctcga acagctcctc ggcgcttccg gcatcggcaa cgacacccac gtcgtgctgt
     4801 acggcgacaa caacaactgg ttcgccgcct atgcgctctg gctcctcgag ctctacggcc
     4861 acgagaacgc ctcgctcatg gacggcggcc gcctcaagtg gatggccgac aaccgccccg
     4921 tcacaaacga cgttccgagc taccccgccg cgagctacag cgcgaaggac gcgaatcccg
     4981 ccctgcgcgc ccagcgcgac gaggtcctgc aggccgtcgg cggtgccgcg agccagctcg
     5041 tggatgtccg tagccccgcc gagtacaacg gcgagatcat cgccccgcca ggcatgaccg
     5101 aaaccgcgca gcgcgctggc cacatccccg gcgcgaagaa catcccctgg gcgcgcgccg
     5161 tcaacgagga cggcaccttc aagtcccccg acgacctcaa ggcgctctac ggcggcgccg
     5221 gcgtcgagga gggaaagccc acgatcgcct actgccgcat cggcgaacgc tcgtcgcaca
     5281 cctggttcgt gctcaagcac atccttggct acggcgacgt caagaactac gacggctcct
     5341 ggaccgagta cggctcgctc atcggcgtcc ccgtcgagaa gtagcggccg aggccggagg
     5401 aacgtcaatg gatgaagagc aggtggccgc cgcggcgctg cagcgagcgc tgctgaaccg
     5461 cgccctcgtc tcgaacgacg ccggccgcct gctcctccgg ttccacgcct ccgtcatgga
     5521 tcgctatcgc gagcagggcg cgacgctgat ccgcacccgg acggttggtc gagtcagcaa
     5581 gcccggccgc tggtcgctcg acgtaggcat cgccgccgga ggcactgagg tacaggtccc
     5641 gttcgacgat ctgctcgagc gtctccccga ggccgagcgc ccgcactggg tcgagcatct
     5701 cgtcgtcgaa ccgtcgagcc tgaacttcct ccgcatgcgc ctcgccggtg ccgcttgcat
     5761 cgatgatggc gaggcagagc cctggacgta gcgggttcgc gaggccgcga gtgaagccgg
     5821 gcgccatcat cgtcattggc ctgggcctgt tcatcatctt cggtctcctc gccgcggggc
     5881 tgtcgctcag cacgacgaag agtcccttcg ccgtcacgac ccaggtgatc gaggcgatgc
     5941 ccgccgagcc gccggcggac gggtcggcat ccgtcgttta tgccaccagc cgcacgagct
     6001 cgggcctcaa gatcctcggc gtgcaggtcc ggcagcccga cttcgccgcc tccgtcggct
     6061 ttgtcccccc gcccggctgt gctcctcccc agggcgtcgt cctcgaggac gagggcctct
     6121 gctcaggcgt cgccctcacg ggggtcgtca tgggcagcgg caccacgagg tcgggccact
     6181 ccctcgtcat cgtgcaggtc gccatctcga gtgcctgcca cgacgtgctg cgcgaaggcg
     6241 acccctggcc ctccacgcac accgcctgcc aggagccgtg acctgttcga ggccctgcga
     6301 ggcatggcga acagcgtccc cgcgccgcac aatcacccca caccgctgca cacgacccgg
     6361 gagctccgat gccgcgcatt cctcgcgccc tgatcgatga gatggtcgcc cacgcccgcg
     6421 aggacctccc caacgaggcc tgtggcatgg tgcacgctcg cgagggcgct ccggtcgccg
     6481 tccaccgcgt caggaatacc accgccagcc cctatcgcta tgagatggat ggccttgagg
     6541 tcgcccgcct cgagcgccag cgcgacgaga gcggggagac actcttcgcc atctatcact
     6601 cccacgtcgc ctccgaggcg cgcccctcga tgaccgacat ccgccaggcg ttctttcccc
     6661 ccggcgagat cgagaccggc ctcttcatgt accccgaggc cctctatatc ctcgtctccc
     6721 tcgccgacga tcctccgccg tccgttcgcg cctggcgcat cgtccgggag gagccccacc
     6781 tcatcgagga gcccatcgag gtcgtgtagg ctggtcgcga tgccgctcga cgccgcgccc
     6841 tgccccgccc ccatccgcat cggcaaccgc accttcgagt ggggcgcgcg cacctacatc
     6901 atggggatcc tcaacgtcac ccccgactcc ttctccggcg acggggtcct cgacccgtcc
     6961 cgtgccgccg aacgg
//
LOCUS       DAORPG010000017         6962 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_497_length_6962_cov_3.470248, whole genome shotgun sequence.
ACCESSION   DAORPG010000017 DAORPG010000000
VERSION     DAORPG010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_497_length_6962_cov_3.470248"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PLX85_00825"
     CDS             complement(<1..718)
                     /locus_tag="PLX85_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC61749.1"
                     /translation="MPPPPLFVRHEVNGLAYGRIDQGDGLAIIGAFPLGITLDGIDHT
                     STQRTFLEALSVGHAFVAYDQRGSGGSAPLGSATDWEQLGADLWAVADAAGFERAILY
                     GVFDAGYTAIQAAAMHPDRVLGLILNFVPPVFGRPEGVPAELMQSWALSTDPVEAQSF
                     DPVHLLQNLGLDEGDAQALGAAWRASTLAQAVQARAALLPQFDPAPLLRECRASGLII
                     EPRRRALFHGWGDSLAQSYTG"
     gene            complement(845..1765)
                     /locus_tag="PLX85_00830"
     CDS             complement(845..1765)
                     /locus_tag="PLX85_00830"
                     /inference="COORDINATES: protein motif:HMM:NF022338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRC61750.1"
                     /translation="MAPTCTGRLILHYQPPRALGLLVGTLLTLWSAGVAVLLANSGIA
                     ADEGWQVFITFLGATLAGALAVLFAFWTYSLATISYLLDRNGLVIAWGPTRQIVPLRA
                     IRRLVPASSVGVPRIRGVSWWGHHVGQGDVAQIGNVLFYSTHQTSDQVLYVVTAEKNY
                     AISVEDPEAFAREVLMRQELGPTAEVTHHVERSGASLESFWADPRARWLAAAAVIGLA
                     LVWAQIALRYGSIPEREPLHFPVNDTVVSSQGREAILELPRTATFLLAADLILGVILH
                     SWDRMAGYVLFAAAVAVEVGIFAAIVLALW"
     gene            1871..2644
                     /locus_tag="PLX85_00835"
     CDS             1871..2644
                     /locus_tag="PLX85_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61751.1"
                     /translation="MAEAGVVGGAAGARSAPRSGLRALHLLLAAQSAIVILVGVNRRT
                     DLTVGYVASNEFLRWVDLINMLALPMASVVASVLLKRWLEARVGGSEASERGERMRWA
                     LPVLDIVFLLGVYVLAASYGTHEVTNYLHVRFCDGDSGRLCEIIAFNDDSFSHWLFFT
                     GFVVINLVILALQALARATPDARGADLALVSVNALFIASGIFVNLAFEETGFDLPVVA
                     LLFVVSAALWWRARRRPLFVYYAVAYGVGLFATGLYKVA"
     gene            2740..3240
                     /locus_tag="PLX85_00840"
     CDS             2740..3240
                     /locus_tag="PLX85_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020290632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HRC61752.1"
                     /translation="MANVETQEHMRYHAAHAHLGTPFGGGWFGAVAERIARFFGTPQY
                     LIGQSLVVALWVASNVVALFHFDPYPFILLNLAFSLQAAYAAPLILLAQTRQAERDKM
                     WSDADARHREELAQNTITLLEQNTDLTEQVAELSRQMRQLTAEIHGAVVMPRREAGGA
                     ERGGSI"
     gene            3257..4042
                     /locus_tag="PLX85_00845"
     CDS             3257..4042
                     /locus_tag="PLX85_00845"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC61753.1"
                     /translation="MPPSILFVPPALNSDPWSLVAPLYDLDFEEFDDDVTMYRELGVR
                     QRGPVLELACGTGRVAAELARAGLDVVGVDFSAGMLGVAAERTRGLPVTLVEGDIRDV
                     RLGRQFGMVLIPLGSLQHLESAADVVAALETVANHLAEDGLAIVDVEAPQPEDFTSGP
                     RPLVAHWTRRWRDSLVTKLVAVDGVPSECRRQVTFHYDIQPNDGPFRRVSHEFNLRTI
                     TSGELELAGRLAGLELTALYGDYELTPLGDGDLRQVAVFELAR"
     gene            4039..5043
                     /gene="plsX"
                     /locus_tag="PLX85_00850"
     CDS             4039..5043
                     /gene="plsX"
                     /locus_tag="PLX85_00850"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRC61754.1"
                     /translation="MTAIALDAMGGDDAPAATVRGAIEAAREGIEVALVGERSVLEAE
                     LARSGGTPPGVRIEQASDVIEMGEHAAIEARRRRDSSIYVGLSLVRRGEADAFVSAGN
                     TGAVFATALVVLGRLRGIERPALGALLPVPAGPTLLLDAGANAECRPSHLVQFAHLGS
                     AYMATVVGVPNPRVGLLNIGEEPTKGTPVLIETHEELAKSDLRFVGNVEGRDVFRGAV
                     DVVVADGFTGNVMLKLAEGLIEMIFTEMRSVASSSLRTRLGGALLRPALREMSTRLDY
                     RKYGAAPLLGVEGPVFVAHGRSDAGTIANAIRTAAGAVERGMIAALAAVPTERLGSRD
                     "
     gene            complement(5050..5388)
                     /locus_tag="PLX85_00855"
     CDS             complement(5050..5388)
                     /locus_tag="PLX85_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HRC61755.1"
                     /translation="MAEPASPEQLRARLSRIEGQIRGIAKMLESERPCEDVVTQLMAV
                     RSGIDTVGALVLDLHLGNCIGCAEPTERVELLRDSMRMWWRFAPGAAGFAVGAVEATE
                     VAPANPEAAN"
     gene            5532..5855
                     /gene="trxA"
                     /locus_tag="PLX85_00860"
     CDS             5532..5855
                     /gene="trxA"
                     /locus_tag="PLX85_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRC61756.1"
                     /translation="MAHPADTTDTTFDADVLQSDTPVLVDFWAPWCGPCRMVAPIVEE
                     LAGEYEGRVKFLKLNTDENPRVASQFGIRSIPTLLVFKQGSIVGQIVGFRPKSDLAKR
                     LDAVL"
     gene            5954..6895
                     /gene="trxB"
                     /locus_tag="PLX85_00865"
     CDS             5954..6895
                     /gene="trxB"
                     /locus_tag="PLX85_00865"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRC61757.1"
                     /translation="MTTNERIDIAIVGGGPAGLAAGLYAARALRRTVLWERGVIGGQI
                     ATTSTVENYPGFAEGVNGLDLALAMRQQAERFGLETRYEAISELRRAGDGFILASAEG
                     EVPARAVIVTAGAEAIRLGVPGEAELTGRGVSYCATCDAAFFRDVPVAIVGGGDAAID
                     EALFTTRYASKVYVIHRRDELRASKVLQERAFASSKIELIWNTVVERVDGEAAVESLQ
                     LRDVRTGERRSLPVAAMFVFVGQRPASELLRGLTRLDAGGHAIVNEWMETDVPGLFAA
                     GDIRAQSARQLVTAAGDGVTAAIRAEHYLAERFDAAA"
BASE COUNT         1064 a         2122 c         2696 g         1080 t
ORIGIN      
        1 cgccggtgta ggactgagcg agtgagtctc cccagccgtg gaagagggcg cggcgccggg
       61 gctcgatgat gagcccggag gcgcggcact cacgaaggag tggggcaggg tcgaactgcg
      121 gcagcagggc ggcccgggcc tggacggcct gggccagggt cgaggcgcgc caggcggcgc
      181 cgagggcctg cgcatcgccc tcgtcgagcc cgaggttctg gagcaggtga accgggtcga
      241 agctctgggc ctcgacgggg tcggtgctca gcgcccagga ctgcatgagc tcggcgggga
      301 cgccctcggg acgcccgaac acgggcggga cgaagttcag gatgagaccg aggacgcggt
      361 cggggtgcat cgccgcggcc tggatcgccg tgtaaccggc gtcgaagaca ccgtagagaa
      421 tggcgcgctc aaacccggca gcatcggcga cggcccagag atcggcgccc agctgttccc
      481 agtcggtcgc cgagccgagg ggcgcggagc caccgctgcc tcgctggtcg taggcgacga
      541 aggcgtgccc aacggagagg gcttccagga aggtccgctg cgtgctggtg tgatcgatgc
      601 catcgagggt gatgcccagc gggaaggccc cgatgatggc gaggccgtcg ccctggtcaa
      661 tgcgaccgta ggcgagcccg ttgacctcgt ggcgcacgaa gaggggcggt ggaggcacgg
      721 gcgcgtggcc atcggcagcc gctgtcggag acgacggcga gggttggtct gctccggcct
      781 cactcatggg ttcaactcca gcacgttggc gtaccgaacg caatggcagg gcatagggtc
      841 ctcatcacca gagcgcgagc acgatcgcgg cgaagatgcc gacctcgacg gcgacggctg
      901 cggcaaacag gacgtagccg gccatgcggt cccaagaatg caggatgacg ccgaggatca
      961 ggtccgcagc gaggaggaac gtggccgtgc gcggcagctc gaggatcgcc tcgcgaccct
     1021 gggaggagac gacggtgtcg ttcacgggga agtgcagggg ttcgcgttcg gggatggagc
     1081 catagcgcag ggcgatctgc gcccagacga gcgcgaggcc gatgacggcg gccgcggcca
     1141 gccagcgcgc gcgcgggtcg gcccagaagg actcgagcga cgcgccggaa cgctcgacgt
     1201 ggtgggtgac ctccgcggtc gggccgagct cctggcgcat cagcacctcg cgggcgaagg
     1261 cttcaggatc ctcgaccgag atcgcgtaat tcttctcggc agtgacgacg tagaggacct
     1321 ggtcgctggt ctggtgcgtt gagtagaaga ggacgttgcc gatctgcgcg acatcgccct
     1381 ggccgacgtg gtggccccac caggagacgc cgcggatgcg ggggacgccg acggaggagg
     1441 cggggacgag gcgtcggatg gcgcgcaggg gcacgatttg cctcgtgggg ccccaggcga
     1501 tgacgaggcc gttgcggtcg agcaggtagg agatggtggc gaggctgtag gtccagaagg
     1561 cgaagaggac ggcgagcgca ccggcgaggg tcgcgccgag gaacgtgatg aagacctgcc
     1621 agccctcgtc ggcggcgatg ccgctgttgg cgagcagcac ggcgacgccg gccgaccaga
     1681 gcgtcagcag ggtgccgacc agaagcccga gcgccctcgg aggctggtaa tgcaagatga
     1741 gccgtcccgt gcaggtgggg gccattcgcg ccccggcccc ccaagtctag ggcggcagcc
     1801 ctcgaatggc acgaccacgc ggatctttag aatgcgctcg tctggagggg tggagcggag
     1861 gaggcgaccg atggcagagg cgggagtggt gggtggcgcg gccggcgcgc ggtcagcgcc
     1921 tcgatcgggc ctgcgtgcgt tgcatctgct gctggcggca cagtcggcga tcgtcatcct
     1981 ggtcggggtc aaccgtcgca ccgacctcac cgtcggctat gtggcgtcga acgagttcct
     2041 gcgctgggtg gacctgatca acatgctggc gctgccgatg gccagtgtcg tcgcctcggt
     2101 gttgctcaag cggtggctcg aggcacgggt cggggggagc gaggcgtcgg agcgcggcga
     2161 gcggatgcgg tgggcgctgc ccgtgctgga catcgtgttt ctcctggggg tgtatgtcct
     2221 cgcggcgagc tacgggacgc acgaggtgac gaactacctg catgtccggt tctgcgacgg
     2281 ggacagcggc cggctctgcg agatcatcgc tttcaacgac gactccttct cgcactggct
     2341 gttcttcacg gggttcgtgg tgatcaacct cgttatcctc gcgctgcagg cgctggcgag
     2401 ggcgacgcca gatgcccgcg gcgccgacct tgcgctggtg agcgtcaacg cgctgttcat
     2461 cgccagcggg atcttcgtga accttgcgtt cgaggagacg gggttcgacc tgccggtggt
     2521 cgcgttgctg ttcgtggttt cggccgcgct gtggtggcgg gcgaggcgac gtccgctctt
     2581 cgtgtactac gcggtcgcat acggcgtcgg gctgttcgcc acgggcctct acaaagtggc
     2641 gtagtggttg ccggtagtcg gcctgtcctc gccagtcgca agctggatcg tgcaccgcag
     2701 ttacctcgag gccgggcaga cggcagggag cgcgacgaga tggcgaacgt tgagacacaa
     2761 gagcacatgc ggtaccacgc ggcgcacgcg cacctcggga cgccgtttgg cggcgggtgg
     2821 ttcggggcgg tcgccgagcg catcgcgcgg ttctttggta cgccgcagta cctcatcggg
     2881 cagtcgctgg tggtggcact gtgggtggcc tcgaacgtgg tcgcgctgtt ccacttcgac
     2941 ccctacccgt tcatcctgct caacctcgcg ttcagcctgc aggccgcgta tgcggcgccg
     3001 ctgatcctgc tcgcgcagac gcgacaggcg gagcgcgaca agatgtggtc ggacgccgat
     3061 gcgcggcatc gcgaagagct ggcacagaac acgatcacgc tgctggagca gaacacggac
     3121 ctgaccgagc aggtcgcgga gctgtcgcgc cagatgaggc aactgacggc ggagatccat
     3181 ggcgcggtcg tgatgccccg gcgggaggcg ggcggagccg agcggggagg gtcgatctga
     3241 attgaccccc cagatcgtgc cgcctagcat cctgttcgtg cccccagccc tcaactctga
     3301 cccctggtcg ctcgtcgcgc cgctatacga cctcgacttc gaggagttcg acgacgacgt
     3361 cacgatgtat cgcgaactgg gggtccggca gcgcggaccg gtgctcgagc ttgcgtgcgg
     3421 gaccgggcgc gtggcggccg aactggcgcg cgcgggcctc gatgtggtgg gcgtggactt
     3481 cagcgcgggg atgctcgggg tggccgcaga gcggacgcgc ggtctgcccg tgacgctggt
     3541 cgagggcgac atccgcgacg tgcgcctcgg ccggcagttt gggatggtgc ttatccccct
     3601 cggcagcctg cagcacctcg agtcggccgc ggacgtagtc gcggcgctgg agacggtggc
     3661 gaaccacctc gcggaggacg ggctggcgat cgtggacgtc gaagcgccgc aaccggagga
     3721 cttcacctcg gggccgcgtc cgctggtggc gcactggacc aggcggtgga gggactcgct
     3781 ggtaacgaag ctggtcgccg tcgatggcgt gccctcggag tgccggcggc aggtgacctt
     3841 ccattacgac atccaaccga acgacgggcc gttccgccgt gtgagccacg agttcaacct
     3901 gcgcacgatc acgagcgggg aactggagct ggccggacgg ctggctggcc tcgagctgac
     3961 cgcgctgtac ggtgactacg aactgacacc gctgggtgac ggcgacctgc ggcaggtcgc
     4021 cgtgttcgaa ctcgcgcgat gacggcgatt gccctcgacg cgatgggtgg cgatgacgct
     4081 ccggcggcga cggtgcgcgg cgccatcgaa gccgcgcgcg aaggtatcga ggtcgcgctg
     4141 gtgggcgagc ggtcggtgct tgaggcggag ctggcacgca gcggcgggac gccgccgggc
     4201 gtgcggatcg agcaggcctc cgacgtgatc gagatgggtg agcatgccgc gatcgaggcg
     4261 cggcgccgcc gcgactcctc gatctacgtg ggactcagtc tcgtgcgccg gggcgaagcc
     4321 gacgcgttcg tctcggcagg caatacgggc gcggtcttcg cgacggccct cgtggtgctg
     4381 ggacggctac gtggcatcga gcgcccggcg cttggcgccc tgttgccggt gccggcgggc
     4441 ccgacgctgt tgctggacgc cggggccaac gcggagtgcc ggccgtctca tctcgtccag
     4501 ttcgcgcacc tcgggtccgc ctacatggcg acagtggtcg gggtgccgaa cccgcgcgtg
     4561 gggctgctca acattggcga ggagccgacg aaggggacgc cggtcctcat cgagacgcac
     4621 gaggagctgg cgaagagcga cctgcgtttc gtcggcaatg ttgaagggcg tgacgtcttt
     4681 cgcggcgccg tggacgtggt ggtcgctgac gggtttaccg gcaacgtgat gctgaagctg
     4741 gccgaaggac tcatcgagat gatcttcacg gaaatgcgta gcgtggcttc gtcgtcgctg
     4801 cgcacgcgcc tggggggtgc actgctgcgg ccggcgctac gtgagatgtc gacgcggctg
     4861 gactaccgga agtacggcgc agcgccgttg ctgggggtgg aggggccggt cttcgtggcc
     4921 catggccgga gtgatgcggg gacgatcgcg aacgcgatcc ggacggccgc gggcgcagtc
     4981 gagcggggaa tgatcgcggc gctggcagct gtccctaccg aacgcctcgg gagccgggac
     5041 tagggtttcc tagttcgccg cctcggggtt tgcgggagcg acctcggtag cctcgacggc
     5101 gccgacggcg aagccggccg cgccgggtgc gaagcgccac cacatgcgca tcgagtcgcg
     5161 caacagctcg acgcgctcgg tgggctcggc acagccgata cagttaccga gatgcaggtc
     5221 gagtacgagc gcgccgacgg tgtcgatgcc ggagcgtacg gccatgagct gcgtgacgac
     5281 gtcctcgcag gggcgctcgc tttcgagcat cttggcgatg ccgcggatct ggccctcgat
     5341 gcgactgagg cgggcgcgga gttgttcggg agaggctggc tcggccatgg gtgtcctccg
     5401 ggcttgacat acccccaagg ggtatctaga atctagcctg tagtagccga ccgtgtcaac
     5461 gcctgcagcg agtacactcg ggcggcacag cttcgaacct cgattacgat gatggcacga
     5521 aaggaagcgg catggcacat ccggcggaca cgaccgacac gacgtttgac gcggacgtat
     5581 tgcagagcga caccccggtc ctggtggact tctgggcgcc ctggtgcggt ccgtgccgca
     5641 tggtggctcc gatcgtggag gagctggcgg gcgagtacga ggggcgcgtg aagttcctca
     5701 agctgaacac cgacgagaac ccccgggtcg cgagccagtt cgggatccgc tcgatcccga
     5761 cgttgctcgt cttcaagcag ggttcgattg tcgggcagat cgtgggcttc cgtcccaaga
     5821 gcgacctggc gaagcgcctg gacgccgtcc tctagtcgcc gctctctctc gagcgtttca
     5881 aacggcgggc ctggcccgcc gtttgtgttt ctccggggcc gctccatcgg cgcgaactca
     5941 ggaggaggag cacgtgacga cgaacgagcg aatcgacatc gcgatcgtcg gtggtggacc
     6001 tgccgggctc gccgcggggc tgtacgcggc acgcgcgctg cgacggacgg tgctatggga
     6061 gcgcggcgtg atcggcgggc agatcgcgac gacctcgacg gtcgagaact acccggggtt
     6121 tgccgagggc gtgaacgggc tggacctggc ccttgccatg cggcagcagg cggagcggtt
     6181 cggccttgag acgcggtacg aggcgatcag cgagctgcgg cgggcggggg acggcttcat
     6241 cctcgccagc gcggagggcg aggtacccgc gcgcgccgtg atcgtgacgg cgggtgcgga
     6301 ggcgattcgc ctcggtgtac cgggggaagc ggagctcacg ggccgaggcg tctcgtactg
     6361 cgccacctgt gacgcggcgt tctttcgcga cgtgccggtg gcgatcgtgg gcggcggcga
     6421 cgcggcgatc gacgaggcac tgttcacgac gcgctacgcc tcgaaggtgt acgtgatcca
     6481 tcgacgcgat gagctgcggg cctccaaggt gctgcaggag cgcgcgttcg cgtcctcgaa
     6541 gatcgagctc atctggaaca cggtcgtcga gcgggtcgac ggtgaagccg cggtcgaatc
     6601 gctgcagctg cgtgacgtcc ggaccggcga gcggcggtcg ctgcccgtcg cggcgatgtt
     6661 cgtcttcgtc ggacagcggc cggcctcgga actgctgcgg ggattgacgc ggctggacgc
     6721 gggcggccat gcgattgtga acgagtggat ggagaccgac gtgcccgggc tcttcgcggc
     6781 cggggacatc cgagcgcagt cggcgcgtca gctcgtgacg gcggcggggg acggagtcac
     6841 cgcggcgatt cgtgctgagc attacctcgc ggagcggttc gacgcggcgg cgtaagggcc
     6901 gctagcggtt gccggcgggg cccggcggca gcgcggggat cccggcgggc aggcccgccg
     6961 cg
//
LOCUS       DAORPG010000018         6927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_503_length_6927_cov_3.689319, whole genome shotgun sequence.
ACCESSION   DAORPG010000018 DAORPG010000000
VERSION     DAORPG010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_503_length_6927_cov_3.689319"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PLX85_00870"
     CDS             complement(<1..840)
                     /locus_tag="PLX85_00870"
                     /inference="COORDINATES: protein motif:HMM:NF016593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HRC61758.1"
                     /translation="MRHIPLTAEPWSQFGPLEAAARLHGRPYLAWLDSALASSARGRW
                     SIVASDPRWIMLAHGSRVSVRDKAGLHCFTADPLRAFASRVEAEVPRVLDPTAPALPF
                     VGGAIGYLGFELGRFVERLPATTIDDVGVPDLAMAWYDAALVWDGLEATGWLVGTPES
                     VCALKARLCRLRRQTEPNHRPSTACDLRSNFSRPRYLDAVRRARAYIEAGDIYQVNLS
                     QRFSAPVHAGGLDLYTRLREVSPAPFAAYLDFRPVPGDPDAHGLEVLSSSPERLLLLD
                     GDRL"
     gene            complement(898..1644)
                     /locus_tag="PLX85_00875"
     CDS             complement(898..1644)
                     /locus_tag="PLX85_00875"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRC61759.1"
                     /translation="MASFDDLDPRLARLLEVGPHATPFVRLPGGRFVMGSDGRKDELP
                     IHEVEVGPVDVALAPVTNAEYAAFLEATGHEPPRFWDDERFNAPQQPVVGTSWYDAVA
                     YCEWLSSLLDRRCRLPSEAEREYAARGGRDGWLYPWGNEALDNGPFAFGSAGMDRPLP
                     VGATSPNGFGLYHMGENVHEWCTDWYDPRGYVEPSTDPAEELPEGARRASRGGSWRHH
                     VKVSRIAARSSLAPDRRYNDYGFRVYADVN"
     gene            1759..2391
                     /locus_tag="PLX85_00880"
     CDS             1759..2391
                     /locus_tag="PLX85_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HRC61760.1"
                     /translation="MPLEIPEVVIDRLPTYYRLLTNLQARGVAVVSSQELGEALNVTP
                     AQIRKDLSYFGRFGKQGRGYSVQRLTDELRTILGLDQRWRMVLIGVGRLGTAIASYRG
                     FEPQGFDIVGVYDVNPEAVGTELGGELVVRNVADLARDLADDPVDIGIVAVPAGEAQA
                     VVDVLVAAGIGAILSYAPTAAQVPAGVEVRQIDPVLSLQGMTYHLRRRDA"
     gene            complement(2413..3948)
                     /locus_tag="PLX85_00885"
     CDS             complement(2413..3948)
                     /locus_tag="PLX85_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61761.1"
                     /translation="MPSMPFRVARSSRRWRSGGRDRVLLLIIAGLGLALVSIYGFLLP
                     LDHSDHPGVAFGYQPLATVMGTDHEGRTRFVAAVLGAYAVFLLAAVLASRLAGRRACA
                     AVLGGTVVLAAVLLPTNPAGAQDVYHNIADARTFWVYGDNPAVTPPIEHRDDPFFKYI
                     PAWPDTPSSYGPLWYVLSGVPLPFTGTSLWLNVLGQKVLTAAFLVLTTALVMLTAARL
                     RPGSAVLAGVLVGWNPLLQFETAGAAHNDIVMVCFAVGAFYALSRRAWDFVFPLLALA
                     VAVKYVLILLAPLFLLWMLLRRDVPRGQLARSVVAGVLLGAAWYVPFLADGALFDTLR
                     GEGNRYLSSTGSFLVSVFMNQRHMGIPEAEQTMKRVLWAAFVASSLLLLTRFRRQPSY
                     GLLIGTSAGVIFLFLVTVKWWFWPWYLTWLVPIAALAPRRGSALLATLFSCTAMLHYA
                     AYYWGLYDEWHHHQRELFAVVFAPPLALAALLVLWTLARGVARSVRRLRRTLLTPAEG
                     VSS"
     gene            4144..5523
                     /locus_tag="PLX85_00890"
     CDS             4144..5523
                     /locus_tag="PLX85_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61762.1"
                     /translation="MAESEGWQPTHRQALLGLFGLGLLVRLALAPYGGHHFDIPTFEF
                     WAYHLVQDPLSQFYHLKQQFMPEQDHLPGDLWLLWFLGQVFRLFSPAMDVETRGFAWM
                     LKLVPALADLGVAWMLYLIGRRLWSRNVGLIAAVLVLFNPASIFLTAVWGQWDSVSTF
                     FALLALWLYLAGRLGWVLPALTYATLIKPQFAAFVPLIALTYYLEYVRREPGDTRPRP
                     EGYWGDVLTPVVAGAAISVALAALVCLPFGVGLLGIGEWSIIERMRIAVNMNQFASLY
                     AFNLWSAVSPPTSQWQIDTEPFFLGASYQAWGTVLTLIVYVAILVAYARDRRPAAMLW
                     AAMAIMLTLYILPTRIHERYMLPVVLMCALPAVIDPIYRRLFAVLSVTYFINIALVYA
                     LPNAPRLFEGASKSGAFWVACSLTNVAVLGVMLWRRAPAAASIPAADTDAAREAGGGE
                     LRGLPVTPV"
     gene            complement(5543..6577)
                     /locus_tag="PLX85_00895"
     CDS             complement(5543..6577)
                     /locus_tag="PLX85_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRC61763.1"
                     /translation="MLTKRLGIDLGTANVLVYEQGRGVVLDEPAVVAITERDNTVVAV
                     GSEAHAMLGRQPATIQVIRPMREGVIADYLITEALLRYLINRVMGRLRVVRPEVMISV
                     PVGVTSVEQRAVRDAAEAAGARRPAHLIPEPLAAAIGAEIPVGSPRGHMVVDIGGGRT
                     EAAVISMFGIVAAESVRVAGDRLNDSIAQYIKRRHNLVVGERTAEEVKIAIGSALPLE
                     REESTGVRGRDQVTGLPRTITVRSSEIVGAIQEHMGAIVQTVRAVLERTPPELASDVI
                     DRGIVLTGGGALLRHLDELLVHETGVPVHVADDPRRCVAKGAGAALGYLDVIARSIPT
                     EEESLIAESR"
     gene            6684..>6927
                     /locus_tag="PLX85_00900"
     CDS             6684..>6927
                     /locus_tag="PLX85_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61764.1"
                     /translation="MQIQPGRLIPLGYGRYVRSDEVAAIEPVTEGRGPQRRALVWVRG
                     LPAPLVASRSEAALAADLVAVEQQRLRTHELRSMIRQ"
BASE COUNT         1138 a         2214 c         2530 g         1045 t
ORIGIN      
        1 gagccgatcg ccgtcgagca gcaggagacg ctcgggggag gacgacaaca cctcgagacc
       61 gtgggcgtcg ggatcgccag gcacgggccg gaagtcgagg taggcggcga agggcgcggg
      121 gctgacctcg cggagccggg tgtacaggtc gagcccgccg gcgtggaccg gagccgagaa
      181 gcgttgcgag aggttgacct ggtagatgtc accggcctcg atgtacgcgc gggcgcggcg
      241 aaccgcgtca aggtaccgag gccggctgaa gttcgagcgc aggtcgcagg ccgtcgacgg
      301 ccggtgattc ggctcggtct gccgccgcag ccggcacagc cgcgctttca gcgcgcagac
      361 cgattcgggg gtgcccacga gccagcccgt cgcctcgagg ccgtcccaga cgagggcggc
      421 gtcgtaccag gccatcgcga ggtcggggac gcccacgtcg tcgatggtgg tcgcgggaag
      481 gcgctcgacg aaccggccca gctcgaaccc gaggtagccg atcgcgccgc cgacgaaggg
      541 gagcgcgggc gcagtcgggt cgaggacgcg cggcacttcc gcctcgacgc ggctggcgaa
      601 ggcgcgcagc gggtcggctg tgaagcagtg caggcctgcc ttgtcacgaa cgctgacgcg
      661 ggagccgtgc gcgagcatga tccagcgagg atccgacgcc acgatggacc agcggccccg
      721 cgcggacgaa gcgagtgcgg aatcgagcca ggccaggtaa gggcggccgt gcaggcgggc
      781 cgctgcttcg agcggtccaa actgcgacca gggttccgcg gtcagaggga tgtgacgcac
      841 tgtcgcccgc ccgccgatcg tgcgtgttga agccctattg taggccgagc ggcggcgcta
      901 gttgacgtcg gcgtagacgc ggaaaccgta gtcgttgtag cgacggtccg gggccaacga
      961 ggagcgggcc gcgatcctcg agaccttgac gtggtggcgc caggagccac ctcgggaggc
     1021 acgacgcgcc ccctccggca gctcttcggc cgggtctgtc gacggctcca cgtaccctcg
     1081 agggtcgtac cagtcagtgc accactcgtg gacgttctcg cccatgtggt agaggccgaa
     1141 cccattcggc gacgtggcgc ccaccggcaa cgggcggtcc atgccggcgg acccgaacgc
     1201 gaacgggccg ttgtcgagcg cctcattgcc ccaggggtag agccaaccgt cccggccacc
     1261 acgcgccgcg tactcgcgtt ccgcctcgct ggggaggcgg cagcgccgat cgaggagcga
     1321 ggacagccac tcgcagtaag ccacggcgtc gtaccaggag gtgcccacaa ccggttgctg
     1381 cggcgcgttg aagcgctcgt cgtcccagaa ccggggcggc tcgtggccgg tggcttcgag
     1441 gaacgcggcg tactcggcgt tcgtgacggg ggcgagcgcc acatcgacag gcccgacttc
     1501 cacctcgtgg atgggcagct cgtctttgcg cccatcggag cccatgacga accggccccc
     1561 ggggagccga acgaatggag ttgcgtgcgg gcccacttcg agcaaccgag cgagccgcgg
     1621 gtcaaggtca tcgaaggagg ccatatgtgc gtgtattcct tcgttatgtc tgagttgcgt
     1681 gaatacccgg tggacgtatg attgtgcaca ctttcgcaag ccgccctcga ctgacatttt
     1741 gacgcccagg gagtgcgcgt gccgctcgag atccccgaag tcgtgatcga ccggctgccg
     1801 acgtattacc ggctgctgac gaacttgcag gcccgtggag tcgcggtcgt ctcctcacag
     1861 gagctcggcg aggcactgaa cgtcacgccc gcgcaaattc gcaaggatct ctcgtacttc
     1921 ggccgatttg gcaagcaggg acgcgggtat tccgtccagc ggctgaccga tgagctgcgg
     1981 acgatcctcg gtctcgacca acgctggcgg atggtgctca ttggcgtcgg gcgcctcggt
     2041 accgcgattg cctcgtatcg tgggttcgaa ccgcaggggt tcgacatcgt cggcgtctac
     2101 gacgtgaatc cggaggccgt cggcaccgag ctcggcggcg aactggttgt gcggaacgtg
     2161 gccgatctgg cgcgcgacct ggcggacgac ccggtcgata tcggcattgt cgcggtgccc
     2221 gcgggcgagg cacaggcggt cgtcgatgtg ctcgtcgcgg ccggcatcgg tgcgatcctc
     2281 agctacgcgc cgacggcggc ccaggtgcca gcaggcgtcg aggtgcggca gatcgacccg
     2341 gtactctcgt tgcagggcat gacttaccac ctgcgccggc gggacgcgta acgcgcgagt
     2401 ccgttccctc ggtcagctgc tgacgccctc ggcgggcgtg agcagggtgc ggcgtagcct
     2461 ccgcacggac cgcgcgacgc ctcgggcgag cgtccagagc acgagcaatg cggcgagcgc
     2521 caggggcggt gcaaagacga ccgcaaacag ctcgcgttga tggtggtgcc actcgtcgta
     2581 gaggccccag tagtacgcgg cgtaatgcag catcgcggtg caggagaaca gcgtcgcgag
     2641 gagcgcggag ccgcgccgtg gggcgagggc cgcaatgggg acgagccagg tcaggtacca
     2701 cggccagaac caccacttca cggtgacgag aaacaggaag atcacaccgg cagacgtgcc
     2761 gatgagcagg ccgtacgagg gctggcgacg gaagcgcgtg agaaggagca gcgaactcgc
     2821 gacgaacgcg gcccacagca cacgcttcat cgtctgttcc gcctcgggaa tccccatgtg
     2881 gcgttggttc atgaagaccg agaccaggaa gctgccggtc gaggagaggt agcgattgcc
     2941 ctcgccgcgg agcgtgtcga acagcgcgcc gtccgcgagg aacggcacat accaggcagc
     3001 gccgaggagg acgccagcga cgacggagcg cgcgagctga cctcggggga cgtcgcggcg
     3061 cagcagcatc cagaggagga acagcggcgc gaggaggatc aggacgtact tcacggcgac
     3121 ggcgagcgcg agcaggggga agacgaagtc ccaggcacgc ctgctgagcg cgtagaaggc
     3181 gccaacggcg aaacacacca tcacgatgtc gttgtgcgcg gcgcccgccg tctcgaactg
     3241 cagcaacggg ttccagccga caagcacgcc ggcgaggacc gcggaacccg gccgaagacg
     3301 tgcggcggtg agcatgacaa gcgctgtcgt cagaacaagg aaggccgcgg tcagcacctt
     3361 ctgaccgagg acgttcagcc agaggctggt accggtgaac gggagcggga cccccgaaag
     3421 cacgtaccag agggggccgt acgaggacgg cgtgtccggc caggcgggta tgtacttgaa
     3481 gaacgggtcg tcgcgatgct cgatgggtgg cgtgacggcg ggattgtcgc cgtagaccca
     3541 gaacgtccgc gcatcggcga tgttgtggta gacgtcctgc gctccagcgg ggttcgtcgg
     3601 caggaggacg gcggcgagga cgacggtgcc tcccagaacc gcggcgcagg cgcggcggcc
     3661 agcgagtcgg ctcgcgagga ccgccgccag gaggaacacg gcataggcgc ccagcaccgc
     3721 cgcgacgaac cgcgtccgac cttcgtggtc tgtccccatg acggtcgcca gcggttgata
     3781 gccgaaggcg acgccggggt gatccgaatg gtcgaggggc agcaggaacc cgtagatgga
     3841 caccagcgcg aggccgagcc cggcgatgat gagcagcaac acccggtcgc ggccaccgga
     3901 gcgccagcgg cgggacgagc gggcgacgcg gaatggcatc gacggcatcg gtttccccgc
     3961 gggaggagga gggacgcgcc tcccgcccgg ctctcagctt gacactggca aacgagctgt
     4021 ggctatggtt ctacgtcccg cgatccatgg ccaccccgag cgatgcaacg agtgagacgc
     4081 gcgaaccgag acgatgcccg acgcgctcgc cccgatggtg gcgagggtga gccggctgcg
     4141 agattggcgg agtcggaggg ctggcagccg acgcaccggc aggcgttgct ggggctcttc
     4201 gggctcgggc tcctcgtgcg gctggcgctc gctccgtacg gggggcacca cttcgacatt
     4261 ccgacgttcg agttctgggc gtatcacctc gtgcaggacc cgctgtccca gttctatcac
     4321 ctgaagcaac agttcatgcc ggagcaggac catctgccag gcgacctgtg gttgctgtgg
     4381 ttccttggcc aggtgtttcg gctgttttcg cccgccatgg acgtcgagac ccgcggcttt
     4441 gcctggatgc tcaagctggt gccggcgctc gctgacctgg gcgtggcgtg gatgctgtac
     4501 ctgatcggcc gtcggctgtg gagccggaac gtgggcctga tcgcggcagt gctggtgctc
     4561 ttcaatcccg cctcgatctt cctgaccgcc gtgtgggggc aatgggactc cgtctcgacc
     4621 ttcttcgcgc tactggcgtt gtggctgtac ctcgcggggc ggctcgggtg ggtgctcccg
     4681 gcgctcacgt acgcgacgct catcaagccg cagttcgccg cgttcgtgcc gctgatcgcg
     4741 ctgacgtact acctcgagta cgtacggcgt gagcccggcg acacacggcc gcgccccgag
     4801 gggtactggg gcgacgtgct caccccggtc gtcgccggag ccgcgatttc ggtcgcactc
     4861 gccgcgctcg tgtgtctgcc gttcggcgtc gggctcctgg gcatcggcga gtggagcatc
     4921 atcgaacgaa tgcgcatcgc cgtgaacatg aaccagttcg cgagcctcta cgcgttcaac
     4981 ctgtggtcgg cggtgtcgcc gcccaccagc cagtggcaga tcgacaccga gccgttcttc
     5041 ctcggcgcga gctaccaggc atgggggacc gtgctcacgc tgatcgtgta cgtagcgatc
     5101 ctcgtggcgt atgcccgaga ccggcgtccg gccgcgatgc tctgggccgc catggccatc
     5161 atgctgacgt tgtacatcct gccgacgcgc attcacgagc gatacatgct gccggtcgtg
     5221 ctgatgtgcg cgctgcccgc ggtgatcgac ccgatatacc gacggctgtt cgcggtgctc
     5281 tccgtaacgt acttcatcaa catcgcgctg gtgtatgcgc tgccgaatgc accgcggctg
     5341 ttcgagggag cgtcgaagtc gggggcgttc tgggtcgcct gttcgctcac gaacgtggcg
     5401 gtgctcggtg tgatgctctg gcgacgcgcg cccgcagccg cctcgatacc ggccgccgac
     5461 accgatgcag cgcgagaagc cggcggagga gagctgcgcg gattgcccgt caccccggtc
     5521 tgagaccagc ctcggtcagc ggtcagcggc tctctgcaat gagggactct tcctcggtcg
     5581 ggatgctgcg cgcgatgacg tcgagatagc caagcgccgc accggcgccc tttgccacgc
     5641 atcgccgcgg gtcgtcggcg acgtgcaccg ggacgccggt ctcgtgcacc agcagttcgt
     5701 cgagatggcg gaggagcgcg ccgccgccgg tgagcacgat gccgcggtcg atgacatcgg
     5761 acgccagctc cgggggcgtg cgctcgagga ccgcgcgcac cgtctgcacg atcgcgccca
     5821 tgtgctcctg gatggcgccc acgatctcgg acgagcggac cgtgatcgtc ctcggcaggc
     5881 cggtgacctg atcgcgtccc cgcacgccgg tcgactcctc acgctcgagc ggcagcgcag
     5941 aaccgatggc gatcttgacc tcttcggcgg tgcgttcgcc cacgacgagg ttgtggcggc
     6001 gcttgatgta ctgggcgatc gagtcgttga ggcggtcgcc ggccacgcgc acggactccg
     6061 ccgcgacgat cccgaacatc gagatgacgg cggcctcggt gcgcccgccg ccgatgtcga
     6121 ccaccatgtg gccgcgcggc gaacctacag ggatctcggc gccaatggca gccgcgagcg
     6181 gctcggggat aaggtgcgcc ggacggcggg ccccggctgc ctcggcggcg tcgcggacgg
     6241 cccgctgttc gacgctcgtg acgccgacgg gcacggagat catgacctcg ggacggacga
     6301 cccggaggcg tcccatgacg cggttgatga ggtagcgcag gagcgcctcg gtgatcaggt
     6361 agtcggcaat gacgccttcg cgcatgggac ggatgacttg aatggtggcg ggctggcggc
     6421 cgagcatcgc gtgagcctcg ctgcccaccg cgacaacggt gttatctcgc tcggtgatgg
     6481 cgacgacggc gggctcatcg aggacaacgc ctcggccctg ctcgtacacg aggacgttcg
     6541 cggtgccgag gtcgatcccg aggcgcttcg tgagcatcga ggacagggta gcgggcggga
     6601 gcgaaggcag cgaggtgcgc ttcgtcacac gataacgtgt ctcgtgatct gagataatgg
     6661 accacagttt gaggaggaga gggatgcaga tccagccagg gcggctgatc ccgctgggct
     6721 acgggcggta cgtgcgctcg gacgaggtcg cggccatcga gccggtgacg gagggccgcg
     6781 ggccgcagcg gcgagcgctc gtgtgggtgc gcggacttcc cgcgccgctc gtcgcttcgc
     6841 gctcggaagc ggcgcttgcc gccgacctcg ttgcggtcga gcagcagcgg ctgcggacgc
     6901 atgaactgcg ctcgatgatc aggcaga
//
LOCUS       DAORPG010000019         6835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_519_length_6835_cov_3.370501, whole genome shotgun sequence.
ACCESSION   DAORPG010000019 DAORPG010000000
VERSION     DAORPG010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_519_length_6835_cov_3.370501"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2167)
                     /locus_tag="PLX85_00905"
     CDS             complement(<1..2167)
                     /locus_tag="PLX85_00905"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HRC61765.1"
                     /translation="MFRRLDPVQHRDRFRHELKMDRDEFAWRQRREMNEDKREDRARE
                     LRLKAQQQQATAAQRAADQREKEAARAEAAAWSRPVTRYTGGTSRGDRHLGEPLIVEH
                     FALPVSVAVLPPVGSGAPPLVSAAEVFLDADPALRDDAEWISTVGKPVHAAFKTVDQR
                     YGGLLSRLRDHNWWRDVCKAAGVVLTSAVDEPRKTPRGLPVTRKVTTHDLPTITDLRI
                     AEDGLRIRIGARVGDTAARWAKGLDTLRAAFKSAGANASKMRVIEDRAGNVVLVLDDA
                     PSTFPKAVAPPAVEPVTSTAQAAKLYPSLVWPLGVDSKGKPITATLDKFPHVLVAGST
                     GGGKSVWLRTVIEALRLQGASVYVADGKRSDYVALAGLPGIRMVSTEPAAHTVAVAEV
                     YETMESRREEASRAKAAGEANPYAKYPPILLVLDEFATMRSDWVGLGGRDKDASVADM
                     IAALLRVGREPRCHVILSSQDIYVENVPQGWQDNMPMFASFGAPSPRTLSSGAIPERL
                     RPDAQRIGDRITRETPGRALYVDRENFLIREVQTYYGYSPGTTSLAPKADPKVAPPTP
                     EVRAAWELASETAEQVPWLYPRVGIKALDGAWRKGALRDIAATPTVALTDRDGNVKPG
                     LEKFDPTNPDWLGAERLGGRKRRADGFDDEPAPLATVTPIKAPRATAPADSGAATATA
                     SDPLAGLVPSAAELAAMSVSERIEVLRLVAQIRGGEAEPA"
     gene            complement(2173..2610)
                     /locus_tag="PLX85_00910"
     CDS             complement(2173..2610)
                     /locus_tag="PLX85_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61766.1"
                     /translation="MKIEINVPDIGTGGLDRFVTRRTVGIAAAVTAACGLWTAWTAAT
                     SVGDALAYVDALILLTAALLLWVLRPDDEPVVDYEDGLRTMSSYHGARYDDGHDDQYQ
                     PPRYEVEQPTYDYPEPTVPEPTPPTPAPILRRGRYDDLLGGDD"
     gene            complement(2607..3455)
                     /locus_tag="PLX85_00915"
     CDS             complement(2607..3455)
                     /locus_tag="PLX85_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61767.1"
                     /translation="MLIRPSSCDAIAANSPSITPPSPPTTTAPAWARSPVPINARPGL
                     TASDFVQCEIGSASGGDADARLERLECMTDRGLIAPVPQYESWKSSVASAESSYASAT
                     SSAASAQSAADTKHRAELDRCTGSRGGLSSTLQVVAFILSIAAAFAGWRRVKEVGEQR
                     ANMQTAFGWDRTYPPGSEIHSGDRELAAAINGAERAAAELEKGSVVWFGGDGSGAEEL
                     RGIALRASREQGNRERGHFAAAPVYAPTEDPEIDLPDGDEDAEVPVAQDASDWVSKLQ
                     SKGDEW"
     gene            complement(3790..4077)
                     /locus_tag="PLX85_00920"
     CDS             complement(3790..4077)
                     /locus_tag="PLX85_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61768.1"
                     /translation="MTTFLPVPLSPSEDLDPITPTLQQVVGVGDPSAAGAGALDRVAA
                     HLAQLGGHVPAGPWYADVSPGAVTALVAITVLLLLIAALVGYGVHQSWSAR"
     gene            complement(4566..5531)
                     /locus_tag="PLX85_00925"
     CDS             complement(4566..5531)
                     /locus_tag="PLX85_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61769.1"
                     /translation="MTNMERNGWDAPTKWFAWAVLLAFTALSAYMNAAYAALSGSVVW
                     FHAGIPGVVLVLGVFLELTYLSSAHRAAKRIVITGLAACFGTVLVASYLAVLSVTTAW
                     NGAAPAWVNAALAGIPDVVMVLAATMILSLRLRTHATAAKAPKKATESRFKRLADAAT
                     ARAESALAVPPSPQVTAVTDVRGRSTEVLTQPHGASVEATAEVSTDDPAGVRGASTST
                     VADAPVESTKATASTPRTPRRTTAKPSVDPELVPFMEQAERMVADGIVARKTPQEIAA
                     VIRAIDEGATDNAIKKAGIASAGTAQKVRAELEYQRAAAPLAAVG"
     gene            complement(5705..>6835)
                     /locus_tag="PLX85_00930"
     CDS             complement(5705..>6835)
                     /locus_tag="PLX85_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61770.1"
                     /translation="DEGIGGRTTGGHEEREGTGGMSTDGFAITRGARREILLRGPLTE
                     QQLGASDEEVAYLDAHSEFQLDAHGRWQARRLPGMTALTPDDRRDAMTAARLLILNLE
                     SVPGGVRDRSAPLSDALGDRAERIYAAARDGLIAAGLVAAERGKLTDRTGDLYRMQRP
                     AWTPKWTQSAVVVPVQEAPAPTPVEPEPTAPKPARVVEKVAAPKPATVEKPAPAPVVK
                     QPPTPVTPDITAAITRLAEASERQAEILSVAVSEGRLERPVRSEHDAARDDLARRLCV
                     LVGAEGQMRRGQITAGRLSKPQRKLASDALTFALGYGALKHSAGWYELGDPTRIGWTR
                     DGFVRAVEEQVEKKRKATERKAAADANAAIAATRDRVTGRAS"
BASE COUNT          954 a         2385 c         2362 g         1134 t
ORIGIN      
        1 gcgcgggttc tgcctcgccg cccctgatct gcgccaccag acggagcact tcgatccgct
       61 cgctgacgct catcgcggcc agctcggcgg cgctcgggac gagtccagcg agcgggtcac
      121 tcgccgtggc ggttgccgcg ccagagtcgg cgggcgccgt ggcgcgcgga gctttgatcg
      181 gcgtcacggt ggcaagcggt gcgggctcgt cgtcgaaccc gtcagcacgt cgcttccgac
      241 cgcccaggcg ttcggcgccc agccaatcgg ggttcgtcgg gtcgaacttc tccagacccg
      301 gcttcacgtt cccgtcgcgg tcggtgagcg ccaccgtcgg ggtcgccgcg atgtcgcgca
      361 acgcgccctt gcgccacgcc ccgtcgagcg ccttgatgcc tacgcggggg tacagccagg
      421 gcacctgctc ggcggtctcg gaagcgagtt cccacgccgc gcgcacctcg ggggtcggtg
      481 gcgccacctt cggatcggcc ttgggcgcga ggctcgtggt accagggctg tacccgtagt
      541 acgtctgcac ctcgcggatc aggaagttct cccgatccac gtacagcgcc ctgccggggg
      601 tctcccgcgt gatgcggtcg ccgatgcgct gcgcatcggg ccgcagccgc tcggggatcg
      661 cgccgctgga cagagtgcgg ggtgacggtg cgccgaacga cgcgaacatt ggcatgttgt
      721 cctgccagcc ctgggggacg ttttccacgt agatgtcctg gctgctcagg atgacgtggc
      781 acctgggttc acgaccgacg cggagcagag ccgcgatcat gtcggcgact gacgcgtcct
      841 tgtcccgccc gccgaggccg acccagtcgc ttcgcatggt ggcaaattcg tcgagcacca
      901 ggaggatcgg cgggtacttc gcgtaggggt tcgcctcgcc cgcagccttc gcccgcgacg
      961 cctcctcacg gcggctttcc atcgtctcgt agacctcggc gacggccacc gtgtgtgcgg
     1021 ccggttcggt gctcaccatg cggatacccg gcagcccggc cagagcgacg tagtccgacc
     1081 gtttgccgtc cgcgacgtac acagatgctc cctgcaggcg cagagcttcg atgaccgttc
     1141 gcagccacac gctcttgccg ccaccagtcg aacccgcgac gaggacgtgc gggaacttgt
     1201 ccagggtcgc ggtgatcggc ttgcccttgc tgtccactcc gagtggccag accagtgagg
     1261 ggtagagctt cgcggcctgc gcggtgctcg tcaccggctc cacggcgggc ggcgccacgg
     1321 ccttcgggaa ggtgctcggg gcgtcgtcga gcaccagcac cacatttccc gcgcggtctt
     1381 cgatcacgcg catcttgctc gcgttcgcgc ccgcgctctt gaacgcggcg cggagggtgt
     1441 ccaagccctt cgcccaccgt gcggcggtgt cgccgacgcg agcgccgatg cggatgcgga
     1501 ggccgtcctc ggcgatgcgg aggtcggtga tcgtcggcag atcgtgagtt gtcaccttcc
     1561 gggtgacggg cagcccgcgc ggcgtcttgc gcggctcgtc cacagcgctt gtgaggacga
     1621 cgcccgccgc cttgcagacg tcgcgccacc agttgtgatc gcgcaagcgg ctcaacagac
     1681 cgccgtaccg ctggtcgacc gtcttgaacg cggcgtgaac cggcttgccc accgtggaga
     1741 tccactcggc gtcgtcgcgc agcgcggggt cggcgtcgag gaagacctcg gcggcgctga
     1801 cgagcggcgg ggctcccgat ccgacagggg gaagcaccgc gaccgagacg ggcagcgcga
     1861 agtgctccac gatcagcggt tcgccgaggt ggcggtcacc gcggctggtg ccgccggtgt
     1921 accgcgtgac ggggcggctc catgccgcgg cctcggcgcg cgcggcttcc ttctcccgct
     1981 ggtcggcggc gcgctgcgcg gcggtcgctt gctgctgctg tgccttcagg cgaagctcac
     2041 gagcgcgatc ctcgcgcttg tcctcgttca tctcccggcg ctgccgccag gcgaactcgt
     2101 cgcgatccat cttcagctcg tgcctgaagc gatcccgatg ctgcacgggg tcgagccgac
     2161 ggaacatgcc catcagtcgt caccccccag gaggtcgtcg taccgcccgc gacgcaggat
     2221 cggcgctggg gtcggtggcg tcggctcggg cacggtcggt tcggggtagt cgtaggtcgg
     2281 ttgttcgacc tcgtaccgtg gcggttggta ctgatcgtcg tggccgtcgt cgtaccgtgc
     2341 gccgtggtac gaggacatgg tgcggaggcc gtcctcgtag tcaacgacgg gctcgtcgtc
     2401 gggccggaga acccaaagca acaacgccgc tgtcagcagg atcagcgcgt cgacgtatgc
     2461 gagagcgtct ccgacgctcg tcgccgctgt ccacgccgtc cagagcccac acgcggcggt
     2521 gaccgctgcg gcaatcccca ccgtgcgccg ggtgacaaaa cggtcgagcc cacccgtgcc
     2581 gatgtcgggc acgttgatct cgattttcac cactcgtctc ccttcgattg cagcttgctc
     2641 acccagtcac tcgcgtcctg cgcgacggga acctcggcgt cctcgtcgcc atcgggcagg
     2701 tcgatctcgg ggtcttcggt gggcgcgtac acaggcgctg cggcgaagtg tccgcgctcc
     2761 cggttccctt gctcgcggga cgcgcgcagg gcgatcccgc gcaactcctc ggcacccgaa
     2821 ccgtccccgc cgaaccacac aacggagccc ttttccagct cagcggcggc gcgttcggcg
     2881 ccgttgatag cggctgccag ctcacggtca ccagagtgga tctcggagcc tggcgggtac
     2941 gtccgatccc agccgaacgc ggtctgcatg tttgctcgct gctctccgac ttccttgacg
     3001 cggcgccatc cagcgaacgc ggcggcgatt gagaggatga acgcgaccac ctgcagggtg
     3061 ctgctgagac caccccgcga ccctgtgcag cggtccagtt ccgcgcggtg cttggtgtcc
     3121 gccgcggact gcgccgacgc cgccgaggag gtggcgctgg cgtacgaact ctcggcgctc
     3181 gccacgctgc tcttccatga ttcgtactgc ggcactggcg cgatgagccc tcgatccgtc
     3241 atgcactcca ggcgctccag gcgcgcgtcg gcgtcgcccc cgcttgcgga accgatctca
     3301 cactgcacga aatcggaggc ggtgagcccg ggtcgcgcgt tgatcggcac cggcgaccgc
     3361 gcccaggcgg gtgccgtggt ggtcggcgga gacggcggcg tgatgctcgg tgagttcgcg
     3421 gcgatcgcgt cgcaggagct cgggcggatc agcatggcca gaatccaggc gccgagagcc
     3481 gcgacggcga gcgcgtgcca gagtcgcggg cgccgtggtg cggggcgcgc agcgtgattt
     3541 gggtgcgtca tgtcgtggtg ctcccaccag tgtccggcga cttagtggac ggcgccgacg
     3601 ggctgctgct gggcgctccc gcgctgggtg ccatttgaat gcagagttgg aaaccagtct
     3661 ggcggttcgt gccgacgacg tacccaggcg ggcactcggc gtcggcgctc gccgggggcg
     3721 cggtcggcac agagacgacc agtccggcgg tgacggccag tgcggcgccg agcgccgcga
     3781 cgcgcgcgct catcgggcgc tccagctctg gtgcactccg tagccgacca acgctgcgat
     3841 gagcagcagg agcacggtga tcgcgacaag cgccgtgacg gcgccagggc tcacgtcggc
     3901 ataccagggg ccagcgggga cgtgaccgcc gagctgggcg aggtgcgcgg cgacccgatc
     3961 cagggcaccg gcaccagcgg cgctggggtc tccgaccccg acgacctgct ggagcgtggg
     4021 ggtgatcggg tccaagtcct cgctggggct caacgggacg ggaaggaagg tggtcatttc
     4081 gaaaacctcc gttttcggat taggtgaagg cggtcccaat accgccctta acctgtgagg
     4141 ttaggatgga tcgctcagct aacctcgaaa tccgtgcgcc ccccggtgac gtgtgctgtt
     4201 cagagacttt tgacgatctg cccagcaaac tcagatccac cgccttttgg gtccacgggc
     4261 agagactcgt ggcagccgtc ggaaacaggg ccagagaact cctatatgcg gcttgggttg
     4321 cagcccaaca cgacccaacg aaaacccaac cgataacgcc gaggatgcgc cgattcgccc
     4381 tgatttcgga cgccgtgggt gttcatatcc atgcagctca cagctagatt tcgagacaac
     4441 caccacccga tttcgccgct tcccgcgatc accgacccaa cggttgggtt ccgaacccaa
     4501 ccgtgagatg cgagaaggcc cgccccggtg acgacggggc gggcctcctg gtcggggtgc
     4561 tgggatcagc cgacggcggc gagcggcgca gcggcgcgct ggtactccag ctcggcgcgc
     4621 accttctgtg ccgtaccagc gctggcgatc cccgccttct tgatcgcgtt gtccgtcgcg
     4681 ccctcgtcga tcgctcggat gaccgccgcg atctcctgcg gggtcttgcg agcgacgatg
     4741 ccgtcggcca ccatgcgctc ggcctgctcc atgaacggca ccagctcggg gtcaaccgac
     4801 ggcttcgcgg tcgtccggcg aggtgtccgc ggagtcgacg cggtggcctt cgtggactcc
     4861 acgggcgcgt ccgcgacggt gctcgtcgag gctccacgga cacccgctgg gtcgtccgtg
     4921 gacacctcgg cggtggcttc cacggatgct ccgtgaggct gcgtgagcac ctccgtggag
     4981 cgtccgcgaa cgtccgtgac ggcggtgacc tgcggcgacg gcgggacggc gagcgccgac
     5041 tccgcgcggg ccgtggccgc gtccgcgaga cgcttgaagc gggactccgt ggccttcttc
     5101 ggagccttcg cggcggtcgc gtgcgtgcgc agccggaggc tcaggatcat cgtcgcggcg
     5161 agcaccatca ccacgtcagg gatgcccgcg agcgctgcgt tcacccacgc gggcgccgct
     5221 ccgttccacg cggtcgtcac ggacaggaca gcgagatagg aggcgacgag gacggtgccg
     5281 aagcacgcgg ccaagcccgt gatgacgatg cgcttcgcgg cccggtgcgc cgacgagagg
     5341 taggtgagtt cgaggaaaac cccgagcacg agaacgacgc cggggatgcc cgcatggaac
     5401 cagacgacgg agccggacag cgcggcgtag gccgcgttca tgtatgcgct gagcgcggtg
     5461 aaggcgagca gcacggccca ggcgaaccac ttggtcgggg cgtcccaccc attgcgttcc
     5521 atgttcgtca cgaccttctc cctctgcgat cctgctttgc tagccatgct ccactcgccc
     5581 tccgacgcct tcggttctcc gtcgacatcc tggcgtcccg acgggctccg atgcacacca
     5641 gattcgggtg cgagtcgccc gtcgaagatc acgtccccgc gctccgcggg gtttgctgcg
     5701 atgctcacga cgccctcccc gtcacccgat cacgggtcgc cgcgatggcc gcgttagcgt
     5761 ccgctgcggc tttgcgctcg gtggctttgc gcttcttctc cacctgttcc tcgacggcgc
     5821 gcacgaaccc atcgcgcgtc cagccgatgc gggttgggtc gccgagttcg taccacccgg
     5881 cgctgtgctt cagcgcgccg tatccgagag cgaacgtcaa agcatccgac gccaacttgc
     5941 gctgcggctt cgacaacctc ccagcggtga tctgtccccg gcgcatctgt ccctcagctc
     6001 ccacgaggac acagaggcgg cgcgcgagat cgtcccgcgc ggcgtcgtgc tcggagcgca
     6061 cggggcgctc caagcgcccc tcggagaccg ccaccgacag gatctccgcc tgccgttcgc
     6121 ttgcctcggc caagcgggtg atcgcggcgg tgatgtcagg ggtgacgggg gtcggcggct
     6181 gcttgacgac cggcgcgggc gcgggcttct ccaccgtcgc gggcttcggt gcggccacct
     6241 tctccaccac cctcgctggc ttcggcgcgg tgggctcggg ctccacgggg gtgggtgccg
     6301 gggcctcctg cacgggaacc accacggcgg actgcgtcca tttcggcgtc catgcgggcc
     6361 gctgcatgcg gtagaggtct ccagtgcgat ccgtcaactt tccacgctcc gctgcgacga
     6421 gccccgccgc gatgagcccg tcccgcgccg ctgcatagat gcgctcggcg cgatcaccga
     6481 gcgcgtcgct cagcggcgcg gaacgatccc gtacgccgcc cggcaccgac tcaaggttga
     6541 ggatcagcaa ccgcgcagcg gtcatcgcgt cgcgccggtc gtcgggggtc agggccgtca
     6601 tgcccggcaa gcggcgcgct tgccagcgtc cgtgcgcgtc gagctggaac tcgctatggg
     6661 cgtcgaggta ggccacctcc tcgtcgctcg ccccgagctg ctgctcggtc agcggcccgc
     6721 gcagcaggat ctcgcggcgc gcgccgcggg tgatggcgaa tccgtcagtg ctcatgccac
     6781 ctgtcccttc tcgttcttcg tggccaccgg tcgtgcggcc accaatgcct tcatc
//
LOCUS       DAORPG010000020         6800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_524_length_6800_cov_3.425352, whole genome shotgun sequence.
ACCESSION   DAORPG010000020 DAORPG010000000
VERSION     DAORPG010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_524_length_6800_cov_3.425352"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="PLX85_00935"
     CDS             complement(<1..129)
                     /locus_tag="PLX85_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRC61771.1"
                     /translation="MLVEYDGTAFAGSQAQPASRRTVQQTLEAAIEAFTGETQRVRF"
     gene            complement(162..512)
                     /gene="rplQ"
                     /locus_tag="PLX85_00940"
     CDS             complement(162..512)
                     /gene="rplQ"
                     /locus_tag="PLX85_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRC61772.1"
                     /translation="MKHGVSGRRFDMPTDQRIAMFRSLVTQVLKHDSIQTTEARAKEV
                     KPLVDKMVTLGKRGTLHARRQALAYVYEPDVVERLFEEIAPRYTTRPGGYTRVMKIGP
                     RKGDGAMMAQLELV"
     gene            complement(509..1615)
                     /locus_tag="PLX85_00945"
     CDS             complement(509..1615)
                     /locus_tag="PLX85_00945"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRC61773.1"
                     /translation="MADLHIPEIRVEAEEADYARIVAEPLEAGFGTTLGNALRRVMLS
                     SLPGAAITSVRIEGVEHEFSTLEHMQEDITEFLLNLKEVRLRAYADRPARLFLEASGE
                     SVVTAGDIQSTADYEVVNPELRLATLTGRDASLVVDVNVESGRGYIPAGATEGLPIGV
                     IPVDAVFTPVRRVNYHVAHTRVGQDTNFDRLEMEIWTDGTITGPEAVSLAAEILREQF
                     LGFQRLSRPETAMSNALQEPSKTFDGYDTPIEDLDLSVRAYNCLKRSGLVTVGAILER
                     SEEELLALRNFGEKSYLELRDKLVSRGFPAPRVDSRRQPVASGISSGNGAPPPALEDD
                     HDTPDDVGSLGAALIQALREAGEDPSELVEDDEE"
     gene            complement(1667..2275)
                     /gene="rpsD"
                     /locus_tag="PLX85_00950"
     CDS             complement(1667..2275)
                     /gene="rpsD"
                     /locus_tag="PLX85_00950"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRC61774.1"
                     /translation="MARYTGPKCRKCRRYGISLCGTAKCALIRRPNPPGPRSLRRRKI
                     SDRGQQLIEKQKVRFAYGLMEKQFRDYYEYAVRRPGVTGELLMRALEERLDNVVYRLG
                     FASTRAQARQLVAHGLVSVDDRKLDVPSAHVKVGSSIGFTDRGKRSEYAKILAEEIKS
                     RDVPGWLELNREALTGRMVAEPGPGDIEGHFNPNAIVEYYSR"
     gene            complement(2288..2683)
                     /gene="rpsK"
                     /locus_tag="PLX85_00955"
     CDS             complement(2288..2683)
                     /gene="rpsK"
                     /locus_tag="PLX85_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRC61775.1"
                     /translation="MGRAQRGGRVRRRERKSVPRGKAFVSSTFNNTIITLTDPNGNVL
                     AWGSGGTAGFKGSRKSTPYAAQIAAEGAAQRAIEHGLREVEVFVKGPGAGREAAVRAL
                     QSSGVRVLSIRDVTPIPHNGCRPRRRPRQ"
     gene            complement(2741..3124)
                     /gene="rpsM"
                     /locus_tag="PLX85_00960"
     CDS             complement(2741..3124)
                     /gene="rpsM"
                     /locus_tag="PLX85_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRC61776.1"
                     /translation="MARIAGVDIPREKQVLFSLPYIYGIGRTRASQILAQTQIEPTTK
                     VRDLTDDEVLRLRNVVEGEYVVEGDLRREVRQNIQRLIEINCYRGYRHRRGLPVRGQR
                     TKTNARTRRGVRRTVSGKRKATRKK"
     gene            complement(3134..3247)
                     /gene="rpmJ"
                     /locus_tag="PLX85_00965"
     CDS             complement(3134..3247)
                     /gene="rpmJ"
                     /locus_tag="PLX85_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006042225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRC61777.1"
                     /translation="MKVSASIKKRCERCQIIRRHGRLRVICVVPRHKQRQG"
     gene            complement(3275..3532)
                     /gene="infA"
                     /locus_tag="PLX85_00970"
     CDS             complement(3275..3532)
                     /gene="infA"
                     /locus_tag="PLX85_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRC61778.1"
                     /translation="MPDRRQQKTTRQPKQKKEVIEVEGVIKDALPNAMFDVELANGHH
                     VLAHVSGRMKMHYIRILLGDRVLVELSPYDLTRGRITYRFR"
     gene            complement(3553..4323)
                     /gene="map"
                     /locus_tag="PLX85_00975"
     CDS             complement(3553..4323)
                     /gene="map"
                     /locus_tag="PLX85_00975"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRC61779.1"
                     /translation="MTAVTIKSKDELAIMREAGRRNAEVRAILRDAVRPGVTGLELDR
                     LAKDEIAKRGAEPTFVGYAPGGRPPYPGAICYSVNDELVHGIPGKRALQEGDIVSIDL
                     GVTYRGYVSDAAFTCAVGDVPEETAELLRVTEASLWAGIDQARVGNYLGDISAAIGAW
                     GERAGYGIIAEYGGHGVGREMHEPPHVLNFGRPGVGHRLKAGMVLALEPMFALGNPDT
                     EEMSDHWTVRMRDGSLSAHFEETIAITEDGPEVLTRIG"
     gene            complement(4342..5010)
                     /locus_tag="PLX85_00980"
     CDS             complement(4342..5010)
                     /locus_tag="PLX85_00980"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRC61780.1"
                     /translation="MQLILLGLPGAGKGTQAQLLAQRQGLLHISTGDMFRQAAAEGTD
                     LGKRAQEYMSRGELVPDEVTIGMLLDRIQRPDAAAGIMLDGFPRTIPQAEALDAALAN
                     QNRAIDAVLYIKVPEDLLMARLTGRLSCPNSGAIYHELTSPPAAKGKCDRCGADLTQR
                     ADDRPDTVRARLDTNRTWTEALADYYEKQGKLHAVDGAGEPDQVTARLTAEIDAVSAA
                     GEKA"
     gene            complement(5010..6353)
                     /gene="secY"
                     /locus_tag="PLX85_00985"
     CDS             complement(5010..6353)
                     /gene="secY"
                     /locus_tag="PLX85_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRC61781.1"
                     /translation="MSREQAASRRPRPQLLQAGIDAFHQTELRQKILFAVAMLIIFRF
                     VAHIPVPGVDSAQLRQTFDNNAILGFMNIFSGGALQNLSVAALGVYPYITASIIMQLA
                     VPLVPRLQSLSKEGESGRQRIQIYTHWITVPLAALQGYAQLLLIQKLGGVSNIGFTGG
                     DALPTIATIFSMVAGTMFLVWLGELISEKGIGNGVSLLIFGGIVAGMPRLLGRGVLTG
                     TGAAIGGLLLLTLIAIAIVAAIVFVQEAQRRIPVQYARSTFRGGRMYRQQGQTHIPLR
                     VNSAGMIPLIFATSIMIFPPTFSQFVAETVDVGWIADLAGWMQTAFSPTGVVYWTAVF
                     FLVVIFSFFYTVVVFQQQNLAENLQRQGGFIPGIRPGRPTQEYITRVLIRITWGGAIF
                     LGLVAVIPFLATRLTNVQALTISAAGMLIVVGVVLDTMRQIEAQMMMRNYEGFIS"
     gene            complement(6353..>6800)
                     /gene="rplO"
                     /locus_tag="PLX85_00990"
     CDS             complement(6353..>6800)
                     /gene="rplO"
                     /locus_tag="PLX85_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRC61782.1"
                     /translation="AGQGTYAGRGRKGQKARGAVRAQFEGGQMPLVRRLGHKRGFRNF
                     NRIEFQAVSLRDLGRRFESGATVDGPSLAAAGLIDHPNAPYKVLASGTIDRALTVSAP
                     RLSEAAKQAISAAGGSFTETAPAEKRVRNRIHRRGATAAPAQEANA"
BASE COUNT         1045 a         2027 c         2603 g         1125 t
ORIGIN      
        1 gaagcgaaca cgctgcgtct cgcccgtgaa ggcctcgatg gccgcctcga gcgtctgttg
       61 caccgtgcgt cgactcgcag gctgggcctg cgagccagcg aaggccgtcc cgtcgtactc
      121 gacgagcagc gcgagccgcc tcgatgcgga cactgcggag gtcagacgag ctcaagctgc
      181 gccatcattg cgccgtcgcc tttgcgcggc ccgatcttca tcacgcgggt gtagccaccg
      241 ggtcgggtgg tgtagcgcgg ggcgatctcc tcgaagagcc gctcgacgac atcgggctcg
      301 tagacgtagg cgagggcctg ccggcgggcg tggagcgtgc cgcgcttgcc cagcgtgacc
      361 atcttgtcga cgaggggctt cacttccttg gcgcgcgcct cggtggtctg gatggagtcg
      421 tgcttgagga cctgcgtgac caggctgcgg aacatcgcga tgcgctggtc ggtcggcatg
      481 tcaaagcggc gaccggagac tccgtgtttc attcctcgtc gtcctccacg agctcagagg
      541 ggtcttcgcc agcctcgcgg agggcctgaa tgagggccgc gccgagtgag ccgacgtcgt
      601 cgggggtgtc atggtcgtcc tcgagagcgg gcggcggagc gccgttgccc gaggagatgc
      661 cggaggcgac gggctggcgc cgggagtcga ctcggggcgc ggggaagccg cgcgaaacca
      721 gcttgtcgcg gagctcgaga taggacttct cgccgaagtt gcggagggcg agcagctctt
      781 cctcggagcg ttcgaggatg gcgccgaccg tgacgaggcc ggagcgcttg aggcagttgt
      841 aggcgcggac ggagaggtcc aggtcctcga tgggggtgtc gtagccatcg aaggtcttgc
      901 tgggctcctg gagcgcgttg gacatggcgg tctcggggcg actcaggcgc tggaagccaa
      961 ggaactgctc gcgcaggatc tcggcggcca gcgagacggc ctcggggccg gtgatggtgc
     1021 cgtcggtcca gatctccatc tcgagacggt cgaagttggt gtcctgaccg acgcgggtgt
     1081 gcgcgacgtg gtagttgacg cggcggacgg gcgtgaagac ggcatcgacc gggatgacac
     1141 cgatgggcag gccctcggtg gcgccggcgg ggatgtagcc gcgcccactc tcgacgttca
     1201 catccacgac gagggaggcg tcgcgaccgg tgagcgtcgc gagacggagc tcggggttca
     1261 cgacctcgta gtcagcggtg gactggatgt ccccggcggt gacgacggac tcgccggacg
     1321 cctcgaggaa gaggcgggcg gggcggtcgg cgtaggcgcg gaggcggacc tccttgaggt
     1381 tgaggaggaa ctcggtgatg tcctcctgca tgtgctcgag ggtggagaac tcgtgctcga
     1441 cgccctcgat gcgcacggag gtgatggcgg cgccggggag tgaggagagc atgacgcggc
     1501 gcagggcatt gccgagcgtg gtgccgaagc cggcctcgag gggctcggcg acgatgcgcg
     1561 cgtagtcggc ctcctcggcc tcgacgcgga tttcggggat gtgcagatca gccaaaaaac
     1621 acctcgctct ggtgagcggc cgccggcggg atgccggcgg cgtggatcac cgtgagtagt
     1681 attcgacgat ggcgttgggg ttgaagtgac cttcgatgtc accgggcccg ggttcggcca
     1741 ccatgcgtcc ggtcagcgct tcgcggttca gttcgagcca gcccggcacg tcacgcgact
     1801 tgatctcctc ggcgaggatc ttggcgtact cggagcgctt gccgcggtcg gtgaagccga
     1861 tgctggagcc gaccttgacg tgagcgctgg ggacgtccag tttgcggtcg tcgacggaga
     1921 cgaggccgtg ggcgacgagc tggcgggcct gggcgcgcgt ggaggcgaac ccgaggcggt
     1981 agacgacgtt gtcgagacgc tcttcgaggg cgcgcatgag gagttcgccg gtgacgccag
     2041 gacggcggac ggcgtactcg tagtagtcac ggaactgctt ctccatgagg ccgtaggcga
     2101 agcgaacctt ctgcttctcg atgagctgct ggccgcggtc agagatcttg cggcggcgca
     2161 gcgagcgcgg tccgggcggg ttaggccgcc ggatgagggc gcacttcgcc gtaccgcaga
     2221 gggaaatccc gtagcggcgg cacttacggc acttgggacc ggtgtaacgg gccatcgatc
     2281 ctcctgatta ctgccgcggc cggcggcgcg gccggcagcc gttgtgcggg atgggggtga
     2341 cgtcacggat ggagagcaca cggacgccgg aggactggag ggcgcgtacg gcggcctcgc
     2401 ggccggcccc ggggcccttc acgaagacct cgacctcacg caggccgtgc tcgatggcgc
     2461 gctgcgcggc accttcggcg gcgatctgcg cggcgtacgg ggtgctcttg cgagagccct
     2521 tgaagccggc ggtgccaccc gagccccacg cgagcacgtt cccattggga tccgtgagcg
     2581 tgatgatcgt gttgttgaag gtggacgaca cgaaggcctt ccctcgcggg acggacttgc
     2641 gttcgcgccg cctgacgcgg ccgcctcgtt gtgctcgacc catacgtact ccctgtcgcg
     2701 cggggcgccg gccggagccg gcaggaccgc tgccgaatgc ttacttcttg cgcgtggcct
     2761 tgcgcttccc ggagaccgtg cggcgcacgc ctcgacgggt gcgggcattg gtcttggtgc
     2821 gctggccgcg aacgggcagg ccgcggcggt ggcggtagcc gcggtagcag ttgatctcga
     2881 tgaggcgctg gatgttctga cgcacctcgc ggcggaggtc accctcgacg acgtactcgc
     2941 cctcgacgac gttgcggagg cgcaggacct cgtcgtccgt gaggtcgcgg accttcgtgg
     3001 tgggctcgat ctgcgtctgc gcgaggatct gcgaggcgcg ggtgcgtccg atgccgtaga
     3061 tgtacggcag ggagaacagc acctgcttct cgcgggggat gtcgacgccg gcgatacgtg
     3121 ccactggagt ctcctaaccc tgtcgctgct tgtgacgcgg gacgacgcag atcacgcgga
     3181 gccgcccgtg gcggcggatg atctggcagc gttcgcagcg cttcttaatc gacgcagata
     3241 ctttcatcgt tcgttcctcg tgctttgcct cgggctaccg gaagcggtag gtgatgcgtc
     3301 cgcgggtgag gtcgtaggga ctgagctcca cgagcacgcg gtctccgagc aggatgcgga
     3361 tgtagtgcat cttcatgcgc ccggagacgt gcgcgagaac gtggtgcccg ttggccagtt
     3421 cgacatcgaa catggcattg ggcagcgcgt ccttgatcac gccctcgacc tcgatgacct
     3481 ctttcttctg tttcggctgt cgcgttgtct tttgctgtct acggtctggc acaggcgttc
     3541 ctcgctcacc cgctagccga tgcgggtgag cacctccggg ccatcctcgg tgatggcgat
     3601 ggtctcctcg aagtgcgcgg agaggctgcc gtcgcgcatc cgcacggtcc agtggtctga
     3661 catctcctcg gtgtccgggt tgccgagagc gaacatcggc tcgagggcca ggaccatgcc
     3721 ggccttgagc cggtgcccga cgccgggacg gccgaagttc aacacgtggg gcggctcgtg
     3781 catttcgcgg ccgacgccgt gcccgccgta ctcggcgatg atgccgtagc cggcgcgctc
     3841 gccccaggcg ccgatggcgg cgctgatgtc gcccaggtag ttgccgacgc gcgcctgatc
     3901 gatgccggcc caaagggagg cctcggtgac gcgcaacagc tcggcggtct cctcggggac
     3961 gtcgccgacc gcgcaggtga acgcggcgtc cgagacgtag ccgcggtagg tgacgccgag
     4021 gtcgatgctg acgatgtcac cctcctggag ggcgcgcttg ccgggaatgc cgtggaccag
     4081 ctcgtcgttc acggagtagc agatggcgcc cgggtatggc ggacgaccgc cgggggcata
     4141 gcccacgaag gtcggctccg cgccgcgctt ggcgatttcg tccttggcca ggcggtccag
     4201 ttcgagcccc gtcactccag gccgcacggc atcgcgaagg atggcgcgca cctcagcgtt
     4261 gcggcgtcca gcctcacgca tgatggcgag ctcgtccttc gacttgatgg tgacggccgt
     4321 cattaccgac atcgatctat gttacgcctt ctcaccggcc gcggacaccg cgtcgatttc
     4381 ggcggtgagg cgggcagtga cctggtcggg ctcgcccgcg ccatcgacgg cgtggagttt
     4441 gccctgcttc tcgtagtagt cagcgagggc ctcggtccaa gtgcggttgg tgtcgaggcg
     4501 ggcgcggacg gtgtcgggcc ggtcgtcggc gcgctgggtg agatcggcgc cgcagcggtc
     4561 gcacttgccc ttcgcggcgg gcgggctcgt caactcgtgg tagatggcac cggagttggg
     4621 gcacgacaag cggccggtga ggcgggccat cagcaggtcc tcggggacct tgatgtagag
     4681 caccgcgtcg atggcgcggt tctggttggc gagggcggcg tcgagcgcct cggcctgcgg
     4741 gatggtgcgc gggaagccgt ccagcatgat gccggcagcg gcgtccgggc gctggatgcg
     4801 gtcgaggagc ataccgatgg tgacctcgtc agggacgagc tcgccgcggg acatgtactc
     4861 ctgggcgcgc ttgccgaggt cggtgccctc ggcggcggcc tgacggaaca tgtcgccggt
     4921 ggagatgtgg aggagaccct ggcgctgcgc gaggagctgc gcctgggtgc ccttaccggc
     4981 gccggggagt ccgagcagga tgagctgcat caggaaatga acccttcgta gttgcgcatc
     5041 atcatctggg cctcgatctg acgcatggtg tcgaggacga cgccgacgac gatcagcatg
     5101 ccggccgcgc taatcgtgag cgcctgcacg ttcgtgagcc tcgtggcgag gaacgggatg
     5161 acggcgacga ggccgaggaa gatggcacca ccccaggtga tgcgaatgag gacgcgggtg
     5221 atgtactcct gggtcggccg tccggggcgg atgccgggga taaagccacc ctggcgctgc
     5281 aggttctcgg caaggttctg ctgctggaag acgaccaccg tgtagaagaa cgagaagatg
     5341 acgacgagga agaacacggc cgtccagtag accacgccgg tgggcgagaa ggccgtctgc
     5401 atccagccgg caaggtcagc gatccagccg acatccacgg tttcggccac gaactgggag
     5461 aacgtgggcg ggaagatcat gatggacgtg gcgaagatga gcgggatcat gccggccgag
     5521 ttcacgcgca gggggatgtg cgtctggccc tgctgccggt acatgcggcc acctcggaag
     5581 gtggagcggg cgtactggac gggaatgcgt cgctgcgcct cctggacgaa gacgatggcg
     5641 gcgacgatcg cgatcgcgat gagggtcagg aggagcaggc cgccgatggc cgcgccagtg
     5701 cccgtgagga cgccacggcc gagcaggcgg ggcatgccgg cgacgatgcc gccgaagatg
     5761 agcagcgaga cgccgttgcc gatgcccttc tcggagatga gttcgccaag ccagacgagg
     5821 aacatcgtgc cggcgaccat cgagaagatg gtggcgatgg tgggcagggc gtcgccaccg
     5881 gtgaagccga tgttggagac gccgccgagc ttctggatga ggagcagctg ggcgtagccc
     5941 tgcagggcgg ccagcggcac ggtgatccag tgggtgtaga tctggatgcg ctggcggccg
     6001 gactcgcctt ccttggagag ggactgcagc ctcggcacca gggggacggc gagctgcatg
     6061 atgatcgagg ccgtgatgta gggatagacg ccgagggcgg ctacggagag gttctgtagc
     6121 gcgccaccgg agaagatgtt catgaagcca aggatggcgt tgttgtcgaa ggtctggcgc
     6181 agctgcgcgg agtcgacacc gggcacgggg atgtgcgcga cgaagcggaa gatgatgagc
     6241 atggcgacgg cgaagaggat cttctgccgc agttccgtct gatggaacgc gtcgataccc
     6301 gcctgcagca gctgcgggcg ggggcgacgg ctggctgcct gctctcgcga catcaggcgt
     6361 tggcctcctg agccggggcg gcggtggcgc cacggcggtg aatgcggttg cggacgcgct
     6421 tctcggccgg ggcggtctca gtgaaggagc cgccggcggc ggagatggcc tgctttgcgg
     6481 cctcggagag gcgcggggca gacacggtca gcgcgcgatc gatcgtaccc gaggcgagca
     6541 ccttgtacgg cgcgttcggg tggtcgatca gaccggcagc ggcgagggag gggccatcga
     6601 cagtggcacc ggactcgaaa cgacgaccga ggtcgcgcag gctgacggcc tggaactcga
     6661 tgcggttgaa gttgcggaag ccgcgcttgt ggccgagacg ccggacgagg ggcatctgac
     6721 cgccctcgaa ctgagcgcgg acggcgccgc gggccttctg gcctttgcgg ccacggccgg
     6781 cgtaggtgcc ctggccggcg
//
LOCUS       DAORPG010000021         6507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_583_length_6507_cov_3.172195, whole genome shotgun sequence.
ACCESSION   DAORPG010000021 DAORPG010000000
VERSION     DAORPG010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_583_length_6507_cov_3.172195"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PLX85_00995"
     CDS             <1..599
                     /locus_tag="PLX85_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRC61783.1"
                     /translation="PGAKERLDRLEEALRLMRGLWDEGEATFEGRYYSLSRATMLPRA
                     TRGRPWVTIGGAGERRTLRLVAEYADEWNCVALTPETYRAKRDILERHCAEVGRDPAA
                     IRRSMMIFGAMGRTNSDVERALSRLPGRVAGPLDSELRARLLARGMLVGTTDEVIDQL
                     GGLAEAGVQEVQLQHLDFDSDEGPEFIAAEIAPRAASL"
     gene            596..1384
                     /locus_tag="PLX85_01000"
     CDS             596..1384
                     /locus_tag="PLX85_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRC61784.1"
                     /translation="MTAWDPALYARFEAERSQPFYDLAALVRARAGMAVIDLGCGSGE
                     LTAWLHRGLEAASTLGIDSSEAMLGRARAQAEPGLTFEAGDINRLESEPWGRRQYDLV
                     FSNAALQWVTDHERLFPRLRSLVAPGGQLAVQMPANEGHISHVTAMDLAREEPYASAL
                     EGYERPLTVLSPVRYAELLHTLGFEEQSVRLQIYPHLLPGPESVVEWVSGTFLTAFRS
                     RLSPELYAAFLEEYGRRLIAALGPARPYLYTYPRVLIWGRLAAG"
     gene            complement(1390..1644)
                     /locus_tag="PLX85_01005"
     CDS             complement(1390..1644)
                     /locus_tag="PLX85_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61785.1"
                     /translation="MASIPQGRALLASQAFTIHDHHASEGTLITRTAWPGELAIDAGP
                     VMRGTRLHADIVMFFAFRDGHIRLQEHVDRDATPEPPPLP"
     gene            complement(1827..2492)
                     /locus_tag="PLX85_01010"
     CDS             complement(1827..2492)
                     /locus_tag="PLX85_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008429890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRC61786.1"
                     /translation="MHTHNDIAPLDLDEEIRKNVLVTSVDGILNWARSSSLWPAMFGL
                     ACCAIEMIATATSRYDIARFGSEVFRASPRQADLMIVAGTVTWKMAPAVRRVYLQMAE
                     PKWVISMGGCAIMGGPFAYGYSVLPGVNLICPVDVYVPGCPPRPESLLQGLMMLQDKI
                     RQHGVEGGQRLRPEPDGDFSRYLPAGDPLRTELESLFPPYLGLDDPNRLAGGYPLAGN
                     IRR"
     gene            complement(2593..3150)
                     /gene="msrA"
                     /locus_tag="PLX85_01015"
     CDS             complement(2593..3150)
                     /gene="msrA"
                     /locus_tag="PLX85_01015"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRC61787.1"
                     /translation="MTGQLERATLAGGCFWCLEAVYEMLRGVEHVTSGYAGGHVPNPD
                     YHSVCTGRTGHAEVVQLEFDPEEITYQDILDVFFTIHDPTTLNRQGPDVGTQYRSAIF
                     THSPEQQEIAEATLHEVDTEGPWDDPIVTEVVPLETFYPAEEYHQQYFRRNPYQGYCQ
                     AIIAPKVAKARSVFLERLKREPSSA"
     gene            complement(3217..4053)
                     /locus_tag="PLX85_01020"
     CDS             complement(3217..4053)
                     /locus_tag="PLX85_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRC61788.1"
                     /translation="MRIEVEHRTRYRFTPPVFLEPHVLRFRPRSNSWQRLDAFEFHLD
                     PQPAGTAANVDLDGNDVVVAWFEGRTEHLVVDVRFEVETLRANAFDFLPANHPSLPMR
                     YEAELDPLLAPYLASESRASLLPYAEAIAEGAAHDATPFAAHLARRLHEDCHHVVRRE
                     GGPRAAEETLRRREGACRDLALLYVELCRTVGLAARFVSGYCLSEEAERDELHAWAEV
                     YLPGGGWRGFDPSTGFAVTDRHIAVAAGPTPLAASPVSGSFRGEAVAESPEIALTIRR
                     RA"
     gene            complement(4055..4828)
                     /locus_tag="PLX85_01025"
     CDS             complement(4055..4828)
                     /locus_tag="PLX85_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HRC61789.1"
                     /translation="MTFCLGISVNDGLVGIADTRVISGNENISARKLSIFHQGRDSFF
                     LMTSGLRSIRDKTVTYLEEDLRGREEPYNRLFKAANAFASEVRRVAAEDGEALAKSGL
                     TFNMHALMGGQLADDPEPKLYLIYPEANWVEVGPSTPYQIIGASGYGKPVLDRTLQYG
                     DSMQFALKVGCLAFDSTRISAADVDFPIDVVLYFAQTREMVVRRFEQDDLREISDGWQ
                     ERLRNSVANLPSPYLDGFWADIAAGRRAWPREVGASTGL"
     gene            complement(4776..5813)
                     /locus_tag="PLX85_01030"
     CDS             complement(4776..5813)
                     /locus_tag="PLX85_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018397439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HRC61790.1"
                     /translation="MLSRVASSVYWLARYIERAENTARWVDVNLQMSLDAPISFSDQW
                     QPLVAITGELPAFLDRYEFATRERVLRYLTTDPDNPSSILSCVRQARENARSIREVIS
                     SEMWEEINHFYLRVSEYAASDHASIDSHDFFNDVKLRSHLVAGVADNTMSHSEAWHFF
                     QLGRLLERADNTSRLLDMKYFLLLPAAAHVGGPIDEMQWSTLLRSASAFEMYRKRYGR
                     IEPEHVVAFLMLDHEFPRAILYCLAHADDSLHAIAGTPIEAIRNPAERLLGRLRAELA
                     FAQASEIIASGLHEYCDRFQARLNEVGSAISTTFFTQPFAQPSAGTAESRSSGDVLSG
                     YQRQRWAGGNS"
     gene            complement(5813..>6507)
                     /locus_tag="PLX85_01035"
     CDS             complement(5813..>6507)
                     /locus_tag="PLX85_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HRC61791.1"
                     /translation="EGRDLVVDDDTLYMRTTHGLERVDVLYRRIDDDFLDPTVFRPDS
                     MLGVPGLMNAYRRGRVALANAPGTGIADDKAIYAYVPEIVRYYTGEDAILPNVPTYLC
                     WRESDLDYVVRHMDELVVKPTNESGGYGMIVGPHATAEEREEFVRRVRANPRNYIAQP
                     VLALSRAPVIVDDHFEGRHVDLRPYILYGEEIYVLPGGLTRVALRKGSLVVNSSQGGG
                     SKDTWVLSGDAA"
BASE COUNT         1058 a         2050 c         2357 g         1042 t
ORIGIN      
        1 ttccgggtgc aaaggagcgc ctcgaccgcc tggaggaggc gctgcggctg atgcgcgggt
       61 tgtgggacga gggcgaggcg accttcgagg ggcgctacta ctcgctgagt agggcaacga
      121 tgctgccgcg ggcgacgcgc ggacggccat gggtgacgat cggcggggca ggcgagcgcc
      181 gcacgctgcg cctggttgcc gagtacgccg acgaatggaa ctgcgtcgcg ctcacgcccg
      241 agacgtaccg cgcgaagcga gacatcctcg aacggcattg cgccgaggtc gggcgtgacc
      301 cggcagcgat ccggcgctcg atgatgatct ttggcgccat ggggcgaacg aactcggacg
      361 tggagcgcgc cctcagcagg ctgcccggac gcgtggccgg cccgctcgac agtgagctgc
      421 gcgcgcggct cctcgcgcgc ggcatgctcg tgggaacgac ggatgaggtg atcgatcagc
      481 tcggcgggct cgcggaggca ggcgttcagg aggtgcagct gcagcacctc gacttcgact
      541 cggacgaagg gccggaattc atcgccgccg agatcgcacc tcgggcggcc tcgctgtgac
      601 cgcatgggat cctgctctgt acgcgcgctt cgaggcggaa cgcagccagc cgttctacga
      661 cctcgcggcg ctggtacgtg cgcgggccgg gatggcggtc atcgacctcg gttgcggcag
      721 cggtgagctg acggcctggc tgcaccgagg gctcgaggca gcgtccacgc tcgggatcga
      781 ctcatcggag gcgatgctcg ggcgggcgcg tgcgcaggca gagccggggc tgacgttcga
      841 ggccggcgac atcaaccgcc tcgagtccga gccgtggggc cgccggcagt acgacctcgt
      901 gttctcgaac gcggctctgc agtgggtgac ggatcacgag cgcctgtttc cccgcctgcg
      961 ctccctggtg gcgccgggcg ggcaactggc ggtccagatg cccgcgaacg aagggcacat
     1021 ctcgcacgtc acggccatgg atctggcgcg cgaggagccg tacgcgagcg ccctcgaagg
     1081 ctacgaacgt ccgctgacgg tgctgtcgcc cgtccgctat gcggagttgc tgcacacact
     1141 gggattcgag gagcagtcgg tgcgactcca gatctacccg catttgctcc cggggcccga
     1201 gtcggtcgtc gagtgggtga gcggcacgtt cctcacggcg ttccgctctc ggctgtcgcc
     1261 cgagctgtat gcagcgtttc tcgaagagta cgggcggcgg ctcatcgcgg ccctcgggcc
     1321 tgcgcggccg tacctctata cgtatccgcg cgtgctgatc tggggacggc tagccgcggg
     1381 gtagcgacct cacggcagag gcggtggttc cggggtcgcg tcgcggtcga cgtgctcctg
     1441 gaggcggatg tgcccgtcgc gaaacgcgaa gaacatcacg atgtccgcat gcaaccgtgt
     1501 cccgcgcata acggggccgg catcgatcgc cagctccccc ggccaggccg tcctcgtgat
     1561 gagcgtgcct tcagacgcgt ggtggtcgtg aatcgtgaag gcctgggatg cgaggagcgc
     1621 ccgtccctgc gggatactcg ccaatgcggt ccgcgcggtc gcgttctgag ccagcgggat
     1681 tcatgaggtt gggatgtttg atgaaccggt agtcgggatc gagcagcgcc tcggcctcgg
     1741 cacgtcggat cccgggttgg cgacgaggct cagatatcgc tcgacgagga ccgtggccgc
     1801 cgcagtatcc acgcgacgcc ggtcgactac cggcggatgt tgccagccag cgggtagccg
     1861 ccggcgaggc ggttcggatc atcgaggccg aggtacggcg ggaagagcga ctccagctcg
     1921 gtgcgcagcg ggtcacccgc cggcaggtag cgagagaagt cgccatccgg ttctggccgc
     1981 aggcgctgcc cgccctcgac gccgtgctgg cggatcttgt cctgcagcat catcagtccc
     2041 tggagcagcg actccggacg gggcggacag ccggggacgt agacgtcgac ggggcaaatg
     2101 aggttcacgc cgggcagcac cgagtatccg tacgcgaacg ggccgcccat gatcgcgcag
     2161 ccacccatcg agatgaccca cttgggctcg gccatctgga ggtagacccg ccgtaccgcg
     2221 ggggccatct tccaggtcac cgtgccggcg acgatcatga ggtcggcctg acgcggcgag
     2281 gcgcggaaca cctcggaccc gaagcgcgcg atgtcgtacc gcgaggtcgc ggtggcgatc
     2341 atctcgatgg cgcagcaggc gaggccgaac atcgccggcc acaacgatga cgagcgggcc
     2401 cagttgagga tgccgtcgac cgatgtgacg aggacgttct tgcgaatctc ctcgtcgagg
     2461 tcgaggggcg cgatgtcgtt gtgcgtgtgc aggtcgagtg agtgggtggt cacggctcga
     2521 tcctagccga gggcgactcg cgcggcaaac gccggacggg cgaagccgcg cgagactgcg
     2581 ctctgctcgg ggtcacgcgg acgagggctc gcgcttcagt cgctcgagga agaccgaacg
     2641 ggccttcgcg accttcggcg caatgatcgc ctgacagtag ccctggtatg gatttcggcg
     2701 gaagtactgc tggtggtact cctcggcggg atagaacgtc tcaaggggga ccacttcggt
     2761 cacgatgggg tcgtcccacg ggccctcggt gtccacttcg tgcagggtcg cctcggcgat
     2821 ctcctgctgc tccggggagt gcgtgaagat cgcggaacgg tactgcgtgc ctacgtcggg
     2881 accttggcgg ttcagcgtgg tggggtcgtg gatggtgaag aagacatcga ggatgtcctg
     2941 gtacgtgatt tcctcggggt cgaattcgag ttgcacgacc tcggcgtggc cggtgcggcc
     3001 cgtacagacg ctgtggtagt cggggttagg gacgtgtccg ccggcgtagc cggacgtcac
     3061 gtgctcgacg ccgcgcagca tctcgtagac cgcctcgagg caccagaagc acccgcccgc
     3121 gagcgtcgct cgttccagtt gtccggtcat cgcgtcctcc tcactcgtcg cgtgtaccga
     3181 ggatacggtc cgttgcttac gtgaagatga ccaaccctag gcccggcggc gaatcgtcag
     3241 ggcaatttcc ggcgactcgg cgaccgcttc gccgcggaag ctccccgaga ccggggacgc
     3301 ggcaagcggc gtggggccag cggcgacggc aatgtggcgg tcagtcacgg cgaagccggt
     3361 cgagggatcg aagccccgcc agccgccgcc cgggagatac acctcagccc aggcatgcag
     3421 ctcatcgcgc tcggcctcct cggagaggca gtagccgctg acgaagcggg cggcgaggcc
     3481 gaccgtccgg cacagctcga cgtacagcag cgcgagatcg cggcaggccc cctcccgacg
     3541 ccgaagcgtt tcctctgcgg cgcgtggccc gccctcgcgc cggacgacgt ggtggcaatc
     3601 ctcgtgcagc cggcgggcga ggtgcgccgc gaacggggtg gcgtcgtgcg ccgcgccctc
     3661 agcgatggcc tccgcgtatg gcagcagaga cgctcgtgac tcgctcgcga ggtagggcgc
     3721 cagcagtggg tcgagttccg cctcatagcg catcggcagc gacggatggt tcgccggcag
     3781 gaaatcgaag gcgttcgccc gcagcgtttc aacctcgaag cgcacgtcca cgacgaggtg
     3841 ctcagtgcgg ccttcgaacc aggccacgac tacgtcgttg ccgtcgaggt cgacgtttgc
     3901 ggccgtgccg gccggctgcg gatcgagatg gaactcgaat gcgtcgagcc gctgccagga
     3961 gttcgagcgc ggtcgaaagc gcaacacgtg cggctcgagg aataccggcg gcgtgaaccg
     4021 atagcgcgtc cggtgttcga cctcgatgcg cacgctagag gccggtcgac gcgccgacct
     4081 ctcgcggcca ggcgcgccgg ccggcggcga tatcggccca aaacccgtcg aggtaggggg
     4141 aggggagatt ggcgacggag ttgcgcagcc gctcctgcca gccgtcggag atctcgcgca
     4201 ggtcgtcctg ctcgaagcgt cggacgacca tctcgcgggt ctgcgcgaag tagaggacga
     4261 catcgatcgg gaagtcgacg tccgccgcgc tgatgcgcgt cgagtcgaag gcgaggcagc
     4321 cgaccttgag ggcgaactgc atcgaatccc cgtactgcag ggtgcggtcg aggaccggct
     4381 ttccgtagcc actcgccccg atgatctggt agggcgtcga tgggcccacc tcgacccagt
     4441 tcgcctcggg gtagatgagg tagagcttgg gctccgggtc atcggcgagc tgaccgccca
     4501 tgagcgcgtg catgttgaac gtgaggccgc tcttggcgag ggcctcgccg tcctcggctg
     4561 ccacgcgacg gacctcggag gcgaaagcgt tcgccgcctt gaagaggcgg ttgtagggct
     4621 cctcgcggcc gcgcaggtcc tcctcgaggt acgtcactgt cttgtcgcgg atggagcgca
     4681 gccccgacgt catcaggaag aagctgtcgc ggccctggtg gaagatcgag agcttgcgtg
     4741 cggagatgtt ctcgttgccc gagatgacgc gggtatcagc tattcccacc agcccatcgt
     4801 tgacgctgat acccagacag aacgtcaccg ctggacctgc tttccgccgt cccggcggac
     4861 ggctgcgcga atggctgcgt aaagaaggta gtggagatcg cgctgccgac ctcattcagt
     4921 cgggcctgga agcgatcgca gtactcgtgc aagcccgagg cgatgatctc agacgcctgg
     4981 gcgaacgcca actcggcgcg gagccggccg agcaggcgtt ccgccgggtt ccggatcgcc
     5041 tcgattggcg taccggcgat ggcgtgcagc gagtcatcgg cgtgggcgag gcagtacagg
     5101 atcgcgcgcg ggaactcgtg gtcgagcatg aggaaggcca cgacatgctc cggctcgatg
     5161 cgcccgtagc gctttcggta catctcgaac gccgaggcgg agcgcagcag ggtcgaccac
     5221 tgcatctcat cgatcggtcc gccgacatga gcggcggccg gcaacagcag gaagtacttc
     5281 atgtccagga ggcgcgaggt gttgtcggcg cgctcgagga ggcggccgag ctgaaagaag
     5341 tgccacgcct cgctgtggga catcgtgttg tcggcgaccc ccgccacgag gtggctccgc
     5401 agcttcacat cgttgaagaa gtcgtgagag tcgatcgagg cgtggtcgga cgccgcgtac
     5461 tcgctgactc gcaggtagaa gtggttgatc tcctcccaca tctcggacga gatcacctcg
     5521 cggatggaac gcgcgttctc gcgggcctgt cgcacacacg agaggatcga gctcggattg
     5581 tcggggtcgg tggtcaggta gcgcagcacg cgctcgcgcg tcgcgaactc ataccggtcg
     5641 aggaacgctg gcagctcgcc cgtgatggca accagtggct gccactgatc cgagaacgag
     5701 atgggcgcat ccagactcat ctgcaggttc acgtcgaccc agcgtgcggt gttctcggcg
     5761 cgctcgatgt aacgcgcgag ccaataaact gagctggcga cgcgactgag catcaggctg
     5821 catccccgga gagcacccag gtgtccttgc tgccgccgcc ctgggaggag ttcacgacca
     5881 gcgacccctt gcgcagcgcc acgcgggtaa ggccgccggg caggacgtag atctcctcgc
     5941 cgtagaggat gtacggccgg aggtcgacat ggcgcccctc gaagtggtcg tccacgatca
     6001 cgggcgcgcg cgaaagggcc aacaccggct gcgcgatgta gttgcgcggg ttggcgcgca
     6061 cgcggcgcac gaactcctcg cgttcctcgg cggtggcatg cgggccgacg atcatcccgt
     6121 agccgccgga ctcgttcgtc ggcttcacga ccagctcgtc catgtggcgc acgacgtagt
     6181 cgaggtcgga ctcgcgccag cagaggtacg tcgggacgtt cgggaggata gcgtcctcgc
     6241 ccgtgtagta acgaacgatc tcgggtacgt aggcgtagat cgccttgtcg tccgcaatgc
     6301 ccgtgccggg cgcgttcgcg agggcgaccc gcccgcggcg gtaggcattc atcaggccgg
     6361 gcacgccgag catcgaatcg ggacggaaga cggtcgggtc gaggaagtcg tcgtcgatgc
     6421 ggcggtagag gacgtcgacg cgttcgaggc catgcgtggt ccgcatgtag agcgtgtcgt
     6481 cgtcgacgac gaggtcgcgt ccctcga
//
LOCUS       DAORPG010000022         6469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_589_length_6469_cov_3.825538, whole genome shotgun sequence.
ACCESSION   DAORPG010000022 DAORPG010000000
VERSION     DAORPG010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_589_length_6469_cov_3.825538"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..2825
                     /gene="ligD"
                     /locus_tag="PLX85_01040"
     CDS             93..2825
                     /gene="ligD"
                     /locus_tag="PLX85_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HRC61792.1"
                     /translation="MTARTRVTRAVLVPGFARPRAAGALTFVVQKHAARRLHYDLRLE
                     WDGVMPSWAVPKGPSLNPADKHLAVHVEDHPLDYATFEGVIPRGEYGGGQVIVWDDGT
                     YSPDEPDLVFHNRDEAERRMRAGLAAGKLSVTFRGKKLKGSWTLVRTNKVESGKEQWL
                     LIKHRDATADATRDLTAEDRSVRSGLSIDDIKGGLLPPAAGRNGVSSVRPEDLPGARV
                     SPFPRDAETMQSESRPTPFSDPRWLFEPKLDGVRTLAFVHDGAVELRSRRGLDNTRQY
                     PSVVSGLKQQPARELILDGEIVALDERGVPSFQLLQERIHLSGDGEISRAEARIPVIY
                     YVFDLLYLDGIDLRAVPLEDRRETLERVLLPMPSIRLVDHFDVDGVDAFEIATSHDLE
                     GLVAKKRDSVYESGRRSRNWLKIKSRNTDEFVVGGFAAGQGGRASTFGSLIVGQYDDA
                     GALHYVTHVGSGFDDRTLAEVNALVRDLTTADCPFPEPPPKNGHPAATWVRPELVVEV
                     EFSHWTRDKRLRAPVFLRVRDDKDPAEIRFPAEPAIAVPKTPVEPGSASLLDAEVARV
                     LTQLEGARKQLTLDVEGHQLRVANLDKVLWPAHDGQRPLTKRDLLVYLTTAAPWLLPQ
                     LRDRPLTLTRYPNGIEGQFFYQKHYEDAPEYVDRIRAYSGTNVRDQTYLLCNNLSTLL
                     WLGQVADLALHTSLARVNPEPDARGLSRDFTGSVDQIEGSVLNYPDFVLFDLDPYIYR
                     GDEGAGAEPELNRAAWQQICEVARWLKELLDSASLSSFVKTSGATGLHVYVPVVRNID
                     YATIRSIAETFASFLIRAHPSAVTMEWAVPKREGKVFLDVNQNARFKNLAAAYSPRAK
                     PGAPVSMPLRWDELEAVYPTDFTILTAPDRLRRVGDLWANILEAKHDLSALLGT"
     gene            2895..4721
                     /locus_tag="PLX85_01045"
     CDS             2895..4721
                     /locus_tag="PLX85_01045"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HRC61793.1"
                     /translation="MSLLRRRSWLQLLPLLLLVAIASALASSPARAQGGVEKIDSYHV
                     DIRIESNGDLTIAEDIAYDFGSEDRHGIYRDVPTKLRYDDEFDRNYPLKVLAVTASDG
                     ASAKYEVEGIEGGKTRIRIGDADKTVTGQHAYHIEYRIQGGLNGFTDHDELYWNAIGT
                     EWSTTIFNPSATVHLPSPIAASDVQVACFKGPRGSSLRCGNATADGAQAQFTNGFLGS
                     YEGLTVVVGFPKGLVPDPKPILDRRWTFQRAFTIDPLRIGLAGALLAVVLAALARLIW
                     SVGRDRRWQGSPVEVVFGGTRQQTVPLFEGGAYAIEFTPPDDLRPGQIGTLIDEVAHP
                     LDVSATIVDLAARGYLRIEEIDKTWWFGKSDWQFVRLPDPASDKRGLLPYERTLLNSL
                     FADGEEVRLSSLKRQFHTELAKVQDQLYTDAVGRGWFNSRPDSTRSSWFLISAVVVAA
                     SIGILVATAKWTTFGIVPIPLVLGSLVLMSQHGRMPRRTAKGTGELRRVLGFRQYIET
                     AETRRSEFAEKANLFYEYLPFAVVFGCVEKWAEAFEGLALEPPDWYSSSRPFNAMMLG
                     NAMSGFSSSTSSTLASTPGGSGGSGSGGSSGGGGGGGGGGSW"
     gene            4779..4854
                     /locus_tag="PLX85_01050"
     tRNA            4779..4854
                     /locus_tag="PLX85_01050"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4812..4814,aa:Phe,seq:gaa)
     gene            5186..6100
                     /locus_tag="PLX85_01055"
     CDS             5186..6100
                     /locus_tag="PLX85_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61794.1"
                     /translation="MRASHRVVRIVLTAALAACSLTAVWRTSASSAPIAVPSASVPTQ
                     VAPADDLLALRDRMAQAIEDYWVPGQYAVAVTDLQTGESISVNGDRPQLSGCVVNLFA
                     LVQALRDVWTARYPIEDVDGLIAATIWSSNAITARDLYGIIGGGDVVSGVQRVARLIN
                     EDLALTAVVLDHPPAFDDSIGLSPDNWVTADAMNAALADLWHGEAIPERSWRDVLLYH
                     MTQVKPGLNYLTAVVPEVVSHKNGFMWTPYGYVDNDVAIVRLQRGDAEYAYAISFLSE
                     GVEDEYADIPLGQQLVFLAYEAMSARYP"
     gene            complement(6114..>6469)
                     /locus_tag="PLX85_01060"
     CDS             complement(6114..>6469)
                     /locus_tag="PLX85_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclase"
                     /protein_id="HRC61795.1"
                     /translation="RAEIGGRDEEWDAEIQEQVPDEKVIWRNTDGRTNAGMVKFDQLG
                     PDRTRVHLEMSYEPEGMVENIGDALGVVSRRVEGDLERFKDYIESRGNESGGWRGEIG
                     NDNAPGGHTSGSMPA"
BASE COUNT         1029 a         2466 c         1900 g         1074 t
ORIGIN      
        1 gctcgatcag cagggtccgg tcggtggccg agcggttgct cacgtcgtac acccgctcct
       61 cccggtggac cgtgtccgtg tggacgaccc ccttgacggc ccgcacgcgg gtgacccggg
      121 ccgtgctggt gcccggcttc gcgcgcccgc gcgcggcagg cgcgctgacg ttcgtggtac
      181 agaagcacgc cgcccgccgc ctccactacg acctccgtct cgagtgggac ggcgtcatgc
      241 cgtcctgggc cgtcccgaaa ggcccctcac tgaatcccgc cgacaagcac ctcgccgtcc
      301 atgtcgaaga ccatccgctg gactatgcga ccttcgaggg agtcatcccc cgcggcgagt
      361 acggtggcgg ccaggtcatc gtctgggacg acggcaccta ctcccccgac gagcccgacc
      421 tcgtcttcca caaccgcgac gaggctgagc gccgtatgcg cgctgggctc gccgcaggca
      481 agctctccgt caccttccga ggtaagaagc tgaaaggctc gtggacactc gtccgtacga
      541 acaaagtcga aagcggcaag gaacaatggc tcctcatcaa gcaccgcgac gcgactgccg
      601 acgccacccg cgacctcacc gctgaggacc gctcggtccg ctccggcttg agcatcgatg
      661 acatcaaggg cggcctgctt ccccccgccg ccggccgcaa cggcgtctcg tcagtccgcc
      721 ccgaggacct tcccggcgca cgcgtctctc ccttcccgcg ggacgccgag acgatgcagt
      781 cggagtcccg ccccacgccc ttcagcgacc cgcgctggct cttcgagccc aagctcgacg
      841 gcgtccgcac cctcgccttc gtccacgacg gcgccgtaga gctgcgctcg cggcgcggcc
      901 tcgacaatac aaggcagtac ccgtcggtcg tctcggggct gaagcagcag cccgcccgag
      961 agctaatcct cgacggcgag atcgtcgccc tcgatgagcg cggggtgcct tcgttccagc
     1021 tgctccagga gcgcatccac ctcagcggcg acggcgagat ctcccgtgcc gaggcgcgca
     1081 tccccgtcat ctactatgtc tttgacctcc tctatctcga tggtatcgac ctccgcgccg
     1141 tgcccctcga ggaccgacgc gaaacactcg agcgcgtcct gctccccatg ccatcgattc
     1201 gcctcgtaga ccacttcgac gtcgacggcg tcgacgcctt cgaaatcgcg acgagccacg
     1261 atctcgaggg cctcgtcgcg aagaagcgcg acagcgttta cgagtcgggg cggcgttcgc
     1321 gcaactggct gaagatcaag tcacgcaaca ccgacgagtt cgtggtggga ggtttcgcgg
     1381 ccggccaggg cggccgcgcg agtacgttcg gctccctcat cgtcggccag tacgatgacg
     1441 ccggagcgct ccattacgtc acccatgtcg gctcgggctt cgacgaccgc accctcgccg
     1501 aggtgaacgc tctcgtccgc gatctcacca ccgccgactg tccgttcccc gagccgcccc
     1561 cgaagaacgg ccaccccgcc gccacgtggg tgcgccctga gctggtcgtg gaggtcgagt
     1621 tcagccactg gacccgcgac aagcgcctcc gcgcccccgt cttcctccgc gtccgcgacg
     1681 acaaggatcc cgccgagatt cgcttccccg ccgagcccgc gatcgcggtc ccgaagacgc
     1741 ctgtggagcc cggctcggca tcgctcctgg acgccgaggt cgcgcgcgtc ctcacccagc
     1801 tcgaaggagc ccgcaagcag ctcacgttgg atgtcgaagg tcaccagctc cgcgtcgcca
     1861 acctcgacaa ggtgctgtgg ccggcgcacg acgggcagcg cccgctgacc aagcgcgacc
     1921 tgctcgtgta cctcaccacc gccgccccct ggctgctccc gcagctgcgc gaccgcccgc
     1981 tcaccctcac ccgctacccc aacggcatcg agggtcagtt cttctaccag aagcactatg
     2041 aggacgcacc cgagtacgtc gaccgcattc gcgcgtactc cggaacgaac gtccgcgacc
     2101 agacgtacct gctctgcaac aacctttcga cactgttgtg gctcgggcag gtcgccgacc
     2161 tcgccctgca cacctcgctc gcccgcgtca acccggagcc cgacgcccga gggctctccc
     2221 gtgacttcac gggctcggtc gatcagatcg aaggatccgt cctcaactac cccgacttcg
     2281 tcctcttcga cctcgatccg tatatctatc gcggcgacga gggggccggg gccgagcccg
     2341 aactgaaccg cgccgcctgg caacagatct gcgaggtggc ccgctggctg aaagagctgc
     2401 tcgactccgc ctcgctctcg tcgttcgtga agacctccgg tgccaccggt ctccacgtct
     2461 acgtgccggt cgtccgcaac atcgactacg ccacgattcg ctccatcgcg gagacgttcg
     2521 cgagcttcct catccgtgcc cacccctcgg ccgtgacgat ggagtgggcc gtcccgaagc
     2581 gcgagggcaa ggtcttcctc gacgtgaacc agaacgcccg cttcaagaac ctggccgccg
     2641 cctattcgcc gcgcgcgaag cccggcgccc ccgtctcgat gcccctccgc tgggatgaac
     2701 tcgaggccgt ctaccccacc gacttcacga tcctcaccgc tcccgaccgc ctgcgccgcg
     2761 tcggcgacct ctgggcgaac atcctcgagg ccaagcatga cctcagcgcc cttctcggca
     2821 cctagacctc agcgatgcgt tcgtcgcctc gatgcggcat catgccccgt gcgtcctgcg
     2881 tcgaaaggcg agccatgtcc ctcctccgac ggcgatcctg gctccagctg ctgccgctgc
     2941 tcctcctcgt ggccatcgcc tcggcgctcg cgtcgtcgcc cgcgcgtgcc cagggcggcg
     3001 tcgagaagat cgactcgtac cacgtcgata tccgcattga gagcaacggt gacctcacga
     3061 tcgccgagga catcgcctac gacttcggct ccgaggatcg tcacggcatc taccgcgacg
     3121 tgccgacgaa gctccgctat gacgatgagt tcgaccgcaa ctacccgctg aaagtactcg
     3181 ccgtcacggc ctccgacggc gcctctgcga aatatgaagt cgagggcatc gagggcggga
     3241 agacccgcat ccgcatcggc gacgccgaca agaccgtcac cggccagcat gcgtaccaca
     3301 ttgagtaccg catccagggc gggctcaacg gcttcaccga ccatgacgag ttgtactgga
     3361 acgccattgg taccgaatgg tccacgacga tcttcaaccc atcggcaacg gtgcacctcc
     3421 cgtcaccgat cgccgccagc gacgtgcagg tcgcctgctt caagggcccc cgaggttctt
     3481 ccctccgctg cggcaacgcg acggcggacg gggcccaggc acagttcacg aacggcttcc
     3541 tcggctccta cgaaggcctc accgtcgtcg taggcttccc gaagggcctc gtgcccgacc
     3601 ccaaaccgat cctcgatcgc cgctggacct tccagcgcgc gttcaccatc gacccgctac
     3661 ggatcggcct cgccggcgcg ctgctcgccg tcgtcctcgc cgcgctggcg cgcctgatct
     3721 ggtccgtcgg ccgcgaccgc cgctggcagg gctcacccgt cgaggtggtc ttcggcggca
     3781 cccgtcaaca gacagtcccg ctgttcgagg gcggcgccta cgccatcgag ttcactccac
     3841 cggatgacct tcgccccggc cagatcggca ccctcatcga cgaggtcgcc cacccccttg
     3901 atgtctcggc gacgatcgtc gacctcgccg cccgtggcta cttgcgcatc gaggaaatcg
     3961 acaagacctg gtggtttggt aaatcggact ggcagttcgt ccgcctgccg gacccggcct
     4021 cggacaaacg aggactcctg ccgtacgaac gcaccctcct caacagcctg ttcgcggacg
     4081 gcgaggaggt gcgcctgtca tcgctgaagc gccagttcca caccgagctc gccaaggtcc
     4141 aggatcagct gtacaccgat gccgtcggtc gagggtggtt caacagccgt ccggattcga
     4201 cccggagctc ctggttcctg atctcggcgg tcgtggtcgc tgcctcgatc ggcatcctcg
     4261 tcgccacggc caaatggacc acgtttggca tcgtgcccat cccgctcgtc ctcggctcgc
     4321 tcgtcctcat gtcccagcac ggacgcatgc cgcgccgcac cgccaagggc acgggtgaac
     4381 tccggcgcgt cctcggcttc cgccagtaca tcgagacggc cgaaacgcgg cgctccgagt
     4441 tcgcggagaa agcaaacctc ttctacgagt acctgccgtt cgccgtcgtg ttcggctgcg
     4501 tcgagaaatg ggccgaggcc ttcgaaggcc tcgcacttga gccgccggac tggtactcct
     4561 cgtcgcgtcc gttcaacgcg atgatgctgg gcaacgccat gtcaggcttc tcgagcagca
     4621 cctcgagcac gctcgcgtcg acccccggcg gctcgggagg cagcggttcc ggaggctcct
     4681 cgggtggcgg cggcggaggt ggcggcggcg gctcctggta acgactggcc ccctccgcaa
     4741 gcccgtcgta tcatccaacc ctgctcgtcg ccgcacgtgc cgaggtagct cagtcggtag
     4801 agcacgcgac tgaaaatcgc ggtgtcgcca gttcgattct ggccctcggc accagacccc
     4861 gcctgcctgc gcccctgcgc ggtcacctca acgcgagcga accgccacgg tccagtcccg
     4921 gctcctctga tatgtcagcg ggcccggcat ccgccatcgc accgccctga ttgcctaccg
     4981 cgccgcccgc cggctcagcc gcctgagatt ccaggacacc cgaggaggga cagcggtacc
     5041 cctctcccct cctctgcgtc cgcgttcgct ccccctttac caccgatgct acagtcggac
     5101 ggttggccgt gatgggccgc ttcgcgccag cagcccgaaa gcccgccggt cggggcatca
     5161 tcggtcgccg agcgaaagga actcgatgcg cgcctcacat cgcgtcgtgc gcatcgtgct
     5221 caccgccgca ctggccgctt gctcactcac cgcggtgtgg cggacgagcg cgtcctccgc
     5281 gcccatcgcc gtgccatccg catccgtccc cacacaggtc gctcctgccg acgacctcct
     5341 cgcgctgaga gaccgcatgg cacaggcgat cgaggactac tgggtaccgg gccaatacgc
     5401 cgtcgccgtc accgaccttc agaccggaga gtcgatcagc gtcaatggcg accggccgca
     5461 gctatccggc tgcgtcgtca atctgttcgc gctggtccag gccctgcgcg acgtctggac
     5521 ggcgcgctac ccgatcgagg acgtcgacgg gctcatcgcc gccaccatct ggagcagcaa
     5581 cgccatcacc gcacgtgacc tctacggcat tatcggcggc ggcgacgtgg tttcaggcgt
     5641 ccagcgggtg gcgcgcctca tcaacgagga tctcgcactg accgcggtgg tcctcgacca
     5701 cccgcccgcc ttcgacgaca gcattgggct cagtccggac aactgggtca ccgccgacgc
     5761 catgaacgcc gccctcgcag acctgtggca cggcgaagcg atccccgagc gctcatggcg
     5821 cgacgtgctg ctctaccaca tgacgcaggt aaagcccggc ctgaactacc tcacagccgt
     5881 cgtccccgaa gtcgtgagcc acaagaacgg cttcatgtgg accccctacg gctacgtgga
     5941 caacgatgtc gccatcgtcc ggcttcagcg cggcgacgcc gaatatgctt acgcgatctc
     6001 attcctctcc gagggtgtcg aggacgagta cgcggacatc cccctcggtc agcaactcgt
     6061 gttcctggcc tacgaggcga tgtccgcgcg ctatccctga cctcggacga caatcaggcc
     6121 ggcatgcttc cactggtgtg gccgccgggc gcgttgtcgt tcccgatctc gccgcgccag
     6181 ccgccgctct cgttacctcg cgactcgatg tagtctttga accgctcgag gtcgccctcg
     6241 acacgccggg acaccacgcc gagcgcgtcc ccgatgttct cgaccatgcc ctcgggctcg
     6301 tagctcatct cgaggtgcac ccgcgtgcgg tcgggcccga gttggtcgaa cttgaccatc
     6361 cccgcgttcg tgcgcccgtc cgtgttgcgc cagatcacct tctcgtccgg gacctgttcc
     6421 tggatctcag cgtcccattc ctcgtcgcgc ccgccgatct ccgcccgcc
//
LOCUS       DAORPG010000023         6426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_600_length_6426_cov_3.383299, whole genome shotgun sequence.
ACCESSION   DAORPG010000023 DAORPG010000000
VERSION     DAORPG010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6426
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_600_length_6426_cov_3.383299"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLX85_01065"
     CDS             <1..184
                     /locus_tag="PLX85_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRC61796.1"
                     /translation="HPVRLIGLGATNLVEDAVQLGFEDGRLLHDQQLDRAIDEIRDRF
                     GNDALSRGLAPRRRWR"
     gene            347..1357
                     /locus_tag="PLX85_01070"
     CDS             347..1357
                     /locus_tag="PLX85_01070"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRC61797.1"
                     /translation="MRYRQIADTEIEVSEVGFGVWTMAAGWWGDYSDEYAVTLLRQAF
                     DRGVTFFDTADTYGNGRGETLLAQAFPGSDRDDIVIGTKFGYDWQARTARRQEGQREA
                     PHRFDVPFLEQALEGSLRRLGTDRIDLWQMHNVRMEHLQRDDVWTFLDKARRDGRVRS
                     VGVALGPAIGWRDEGLYALRQLPVDVVHMIYNALELDPGRDLIRAASDTGRSLLVRVP
                     HSSGMLEGRYTEETQFSERDHRSHRPRAWLTNGLRKVEQLDFLTIGREMTLAQAALRF
                     VLHDPAVASALPNIYDRDQLEEFVAAADCPDLTPGDVERIESLFESNYGLPREREQAV
                     AR"
     gene            1354..2433
                     /locus_tag="PLX85_01075"
     CDS             1354..2433
                     /locus_tag="PLX85_01075"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRC61798.1"
                     /translation="MTTEARTRPNPTLDEMTARVLNELQDRFPLAERPYLEVGARLGI
                     SESETVARLREARAAGVIRQVCGIYDTKSLGYSSALVAMRVSAERLERAAAVVNAHPG
                     VSHNYQRNHPFNMWFTVAMPPGHDLDAVIDRLHQLSGAESTRPMPTLRLFKIGVTLDI
                     AGTRPAAARGEPEYSDERRNRAPRHEIDGDDIAYIRATQGDLPDVERPFDAVAETLGC
                     SLEAVLEHGRRLTASGHLRRFAAILNHRAAGFRANGMAVWAVPTDRIEEFGTFVAGYR
                     NVSHCYQRPTYEDWPYNVFSMIHSPRVDGVEEVAEAIAAETGVTDRRVLYSSIEFKKI
                     RLPYFVPEYEQWESLCTAAGEEAHP"
     gene            2430..3191
                     /locus_tag="PLX85_01080"
     CDS             2430..3191
                     /locus_tag="PLX85_01080"
                     /inference="COORDINATES: protein motif:HMM:NF018484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HRC61799.1"
                     /translation="MSGHPHATQPAGNEAPRATGWGRQFVKFSFYRLRDELRQADAAT
                     RAEAGACLDALLCGSAERMLTRTYSTVGTRRDTDLLVWQVADELDTITDWHASLLNSP
                     VGPQLERSASYLSMTMRSLYENPLHDGASGRDRLRADESGARYLFVYPMVKTREWYRL
                     PKSERQRMMDEHIAIGHAYHGIRINTTYSYGLDDQEFVVAFEGDHPGEFLALVRELRE
                     SEASAFTERDTPMYTCRRMPLDNLLRHVGLAGVAT"
     gene            3281..3916
                     /locus_tag="PLX85_01085"
     CDS             3281..3916
                     /locus_tag="PLX85_01085"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC61800.1"
                     /translation="MDQTLTNGTAGSTRWVNLGRACEILGVNESTVRRWADSGEIRCF
                     RTPGGHRRFAESDLYAMTESGGRRPESDLETAAVSRIRRQLHAGSKSGPAGWYATLEE
                     EERDVLRPLGRRLVELVGDYISRRGRRAQVEHEVDEIGRRYGQILHERGMPLMRAVQA
                     FTFFRRSLDETAKRLAERNSMTGEEAARAREDIAGLADRVLLGLAAAYDPR"
     gene            3923..4633
                     /locus_tag="PLX85_01090"
     CDS             3923..4633
                     /locus_tag="PLX85_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HRC61801.1"
                     /translation="MGYYPIFVEMNGRSVLLVGGGHVALEKIGKLVQAGARVTVVAPE
                     LIAPIRAFIDRGDASWRQRRYEPGDTEGFDLVMVATDDGEVNSTVAAEARAAGIWVNA
                     ADDVRNCDFILPALAQRGTIAIAASTGGSSPALARWLRERLEEFLSDEVVALGDLLAD
                     VRRLARERDRACAATCDRTRTPPPLLCGSCPNRITPDAWQAAIDDELRALLLQGEYEA
                     ARARLVRTLGIDRPLVQP"
     gene            4647..5915
                     /gene="hemA"
                     /locus_tag="PLX85_01095"
     CDS             4647..5915
                     /gene="hemA"
                     /locus_tag="PLX85_01095"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRC61802.1"
                     /translation="MLTLVGLSHQTAALEVREQLAIPADELPATLGALRERFGAGAIL
                     ATCNRLELYLPGDHAREDVLAFLSDQAGLDVSLAAAHFAALHDRDAVRHLYSVAGGID
                     SMVLGESEVLGQVRAAFSASVAAGCDDALLSRLFHTAIRLGRRARTETAIGRHALSVS
                     SISVQQARLLCPELPEATVLVIGAGEAGRLAAAALVEQGVRDVLVANRTRERAEDLAA
                     ELGGVACSLDELDEALPRADVVIAAADAPSPLVTRAMVDAAMEQRRRRPLLLVDIGVP
                     RDIDHTVGELPGVTYYDLDGLQAIAAEHYAVRAGEVATVQTMVDEETDRFVEWWEQLQ
                     VVPTIAALTDRAERLRSTELAKTLRRLNVGDEERAHLEALTRALVKQILHDPIATLRE
                     RGDREVYIDAVRKLFRLDDEPGDLENRELS"
     gene            complement(5840..>6426)
                     /locus_tag="PLX85_01100"
     CDS             complement(5840..>6426)
                     /locus_tag="PLX85_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61803.1"
                     /translation="ATILSHAGLDPTVVIGGTLFLLDKTYAGGELHGIGGPKLAAPQA
                     TLLMDPMPYRRLVRRAGWSFEDYVAWVAAFEERPEHESLRNRGRHVFERVHSDVRAPL
                     EERFLDRTDEDAAPAEDAQGCAAVRVALGAHLDELDLDRRVGRTQGGSDQLALGERES
                     GAARREPEVGQLSSRFSRSPGSSSSRKSFRTASM"
BASE COUNT         1005 a         2309 c         2147 g          965 t
ORIGIN      
        1 ccatcccgtc cggctgatcg gcctcggcgc gacgaatctc gtcgaggacg ccgtccagct
       61 cggcttcgag gatggccgcc tcctgcacga ccagcagctc gaccgcgcca tcgacgagat
      121 ccgcgaccgc ttcggcaacg acgcgttgtc gcgcggcctc gcgccaaggc gccgctggcg
      181 ctgaacggcc cgcgccggca aaccccgcga acctgaccaa actcgaatct cacttacctg
      241 cgcgaaggtt cgaggaatcg ccgcgagtct gacaaagcct cgacaagtcc gccttgcccc
      301 tctccggagc ctgcgtttcg ctgcccatga gccgagggag gacgccatgc ggtaccgcca
      361 gattgcggat acggagatcg aggtttcgga ggttggtttt ggtgtctgga cgatggccgc
      421 gggctggtgg ggcgactact cggacgagta cgccgtcacc ctcctccggc aggcgttcga
      481 ccgtggggtg accttcttcg acacggctga cacctatggc aacgggcgcg gcgagacgct
      541 gctggcgcag gcgttccctg gcagcgaccg cgacgacatc gtcatcggca cgaagtttgg
      601 ctacgactgg caggcgcgga cggcgcgccg ccaggagggc cagcgcgagg cgccgcaccg
      661 cttcgatgtc cccttcctgg agcaggccct cgagggctcg ctgcgccgcc tcggcaccga
      721 ccgcatcgat ctctggcaga tgcacaacgt gcgcatggag cacctccagc gcgacgacgt
      781 gtggacgttc ctcgacaagg cccggcgcga cggcagggtg cgctccgttg gcgtcgcgct
      841 cggcccggcg atcggctggc gggacgaggg gctctacgct ctccggcaac tgcccgtcga
      901 cgtggtgcac atgatttaca acgcgctcga gctggacccc ggacgcgacc tgatccgggc
      961 ggcgagcgac acgggccggt ccctcctcgt acgcgtgccc cactcctccg ggatgctcga
     1021 gggccgctac accgaggaaa cgcagttctc cgagcgcgac catcgcagcc atcgccccag
     1081 ggcctggctc acgaacggcc tgcgcaaggt cgagcagctc gacttcctga ctatcggccg
     1141 cgagatgacc ctcgcccagg cggcactgcg tttcgtcttg cacgatcccg ccgtcgccag
     1201 cgccctcccc aacatctacg accgcgatca gctcgaggag ttcgtagccg ccgcggactg
     1261 ccccgacctc acacccgggg acgttgagcg catcgagtcg ctgtttgagt cgaactacgg
     1321 gctcccccgc gagcgagagc aggcggtggc acgatgacta cggaggctcg aacccgtccc
     1381 aaccccaccc tggacgagat gacggcccgg gtgctgaacg agctgcagga ccgctttccg
     1441 cttgcggagc gcccgtacct tgaggtcggc gcacggctcg gaatttccga gtcagagacc
     1501 gtcgcgaggc tacgcgaggc gcgcgcggcc ggagtcatcc gccaggtctg cggcatctac
     1561 gacacgaaga gcctcggcta ctcgtccgcc ctggtcgcga tgcgggtcag cgctgagcgc
     1621 ctggaacgcg cggcggccgt cgtgaacgcg catcccggcg tctcgcacaa ctaccagcgc
     1681 aatcacccgt tcaacatgtg gttcacggtc gccatgccgc cgggtcacga cctcgacgcg
     1741 gtcatcgacc gcctccacca gctgtccggg gccgaatcga cgcggccaat gcccacgctg
     1801 cggctcttca agatcggcgt cacgctcgac atcgccggga cgaggcccgc cgcagcccgt
     1861 ggtgaacccg agtactcgga cgaacggcgc aaccgcgcgc cgcggcacga aatcgacggg
     1921 gacgacatcg cgtacatccg ggccacgcag ggcgacctcc cggacgtgga gcgtcccttt
     1981 gatgccgtcg ccgagacgct cgggtgttcg ctcgaagcag tgctggagca cggccggcga
     2041 ctgacagcca gcggccacct tcgccgtttc gccgccatcc tcaatcatcg cgcggccggc
     2101 ttccgcgcga acgggatggc cgtctgggcg gtccccaccg atcggatcga ggagttcggc
     2161 acgttcgtcg cgggctaccg caacgtgtcg cactgctacc agcggcccac gtacgaggac
     2221 tggccctaca acgtcttctc gatgatccac tcgccgcgtg tcgatggcgt cgaggaggtg
     2281 gctgaggcga tcgcggctga gacgggcgtg accgaccgcc gcgtcctcta ctcgagcatc
     2341 gagttcaaga agattcgcct cccgtacttc gtccccgaat acgagcagtg ggaatcgctg
     2401 tgcacagcag ccggcgagga ggcgcatccg tgagcggcca tccacacgcg acacagccag
     2461 cgggcaacga ggcgcctcgc gccaccggat ggggacgcca gttcgtcaag ttcagctttt
     2521 accgtctgcg cgatgaattg cggcaggccg acgctgccac acgagccgag gcaggcgcct
     2581 gcctcgatgc actcctctgc ggatcagcag agcgcatgtt gacccggacc tactcgaccg
     2641 tcggcacgag gcgcgacacc gacctcctcg tctggcaggt cgccgatgag ctcgacacga
     2701 tcacggactg gcacgcgtcg ctgctgaact cgcctgtcgg cccgcagctc gagcgttccg
     2761 cctcgtacct ctcgatgacg atgcgctcgc tctacgagaa cccgctgcac gatggcgcct
     2821 cggggcgtga ccggctccgc gcggacgaga gcggcgcgcg ctatctcttc gtgtatccga
     2881 tggtgaagac gcgcgagtgg taccggctgc cgaagtcgga acgccagcgc atgatggacg
     2941 agcacatcgc catcggacac gcgtaccacg gcatccgcat caatacgacc tactcgtacg
     3001 gtctggacga ccaggagttc gtcgtggcct tcgaaggcga tcaccccggt gagttcctcg
     3061 ccctcgtccg cgagctgcgc gagtccgagg cgtcagcctt cacggagcgg gacaccccga
     3121 tgtacacgtg ccggcgcatg ccgctggaca acctcctgcg ccatgtcggg ctggcgggcg
     3181 tcgccacgtg atggccgcga gcccgcgcga ggttgcgccg ccgggtggcc ggaaatacac
     3241 tgcagatctt gcagacttcg accagcattt tggtgagatt ttggatcaga cgctcacgaa
     3301 cgggacggct ggatccaccc gctgggtaaa cctcgggcga gcgtgcgaga tcctcggagt
     3361 gaacgagtcc accgtccgcc gctgggctga ctccggcgaa atccgctgct tccgcacccc
     3421 cggcggccac cgtcgcttcg ccgagagcga cctctacgcc atgaccgaga gcggcggccg
     3481 ccgccccgag agcgacctcg aaactgccgc cgtctcccgc atccgccgtc agctgcatgc
     3541 ggggagcaag agcggccccg ccggctggta cgcaaccctc gaggaggagg agcgcgacgt
     3601 cctgcgcccg ctcggacggc ggctcgtcga gctcgtgggc gactacatct cacgccgagg
     3661 ccgtcgagcg caggtggagc acgaggtcga cgaaatcggc cgccgctacg gtcagatcct
     3721 gcacgaacgc ggcatgcctc tcatgcgtgc cgtgcaggcg ttcacgttct tccgacgctc
     3781 gctcgacgag actgcgaagc ggctcgccga acgcaatagc atgaccggcg aggaggccgc
     3841 gcgtgcccgt gaggacatcg cgggcctcgc cgaccgcgtg ctactcggcc tcgcggccgc
     3901 gtacgatcct cgatagctcg ctatggggta ctacccgatc ttcgtcgaga tgaacggtcg
     3961 ctcggtgctc ctcgtgggcg gaggacacgt ggcgctcgag aagatcggga agctcgtgca
     4021 ggccggagcg cgcgtgaccg tggtcgcgcc cgagttgatc gccccgatcc gtgcattcat
     4081 cgaccggggt gatgcgtcgt ggcgccagcg acgctacgag cccggcgaca ccgaggggtt
     4141 tgacctcgtc atggtcgcga ccgatgacgg cgaggtgaac tcgaccgtcg ccgccgaggc
     4201 gcgcgccgcc ggaatctggg tcaacgctgc cgacgacgtc aggaactgcg acttcatcct
     4261 cccggctctc gcccagcgtg ggacgatcgc gatcgccgcc tcgaccggcg gctccagccc
     4321 cgcgctcgca cgctggcttc gggaacgcct cgaggagttc ctgagcgacg aggtcgtggc
     4381 cctgggcgac ctactcgccg atgtccgccg cctcgcacgt gagcgcgatc gcgcctgtgc
     4441 agccacttgc gaccgcactc gcacgcctcc tccgttgctc tgcggcagct gccccaaccg
     4501 catcacgccc gacgcatggc aggcggccat cgacgacgaa ctgcgcgcgc ttcttctcca
     4561 gggcgagtac gaggccgcga gggcgcgcct cgtgcgcacg ctgggcatcg accgtccgct
     4621 ggtgcagccg tgagcgaggc gccgcgatgc tgacactcgt tggcctcagc caccagaccg
     4681 cagccctcga ggtacgggaa cagctcgcga tccccgcgga cgagctgccc gcgacgctgg
     4741 gcgcgttgcg cgaacgcttt ggcgcgggcg cgatccttgc tacctgcaac cgcctcgagc
     4801 tctatcttcc cggcgaccac gcccgcgaag acgtgctcgc cttcctgtcg gaccaggcgg
     4861 gcctcgacgt gtcgctcgcc gcggcgcatt tcgcggcact ccacgaccgc gacgcggtgc
     4921 gccacctcta ttccgtcgcc ggcggcatcg actcaatggt gctgggcgag tccgaggtgc
     4981 tgggccaggt gcgcgccgcc ttctcggcct cggtcgccgc cggctgtgac gacgcgctgc
     5041 tctcgaggct cttccacacg gcgatccgcc tgggacggcg cgcccgcacg gagaccgcca
     5101 tcgggcgcca cgccctgtcc gtctcctcga tctcggtgca gcaggcgcgc ctgctgtgcc
     5161 cggagcttcc cgaggcgacc gtcctcgtca tcggcgctgg ggaggccggc cggctcgcgg
     5221 cggccgcgct cgtcgagcag ggtgtgcgag acgtgctcgt cgcgaaccgc acccgggagc
     5281 gcgccgagga cctcgcggcc gaactcggcg gcgtcgcctg ttcgctggac gaactggacg
     5341 aggcgctgcc gcgggcggac gtcgtgatcg cggccgccga tgcgccctcg cccctggtga
     5401 cccgcgccat ggtcgacgcg gcgatggaac agcgccgtcg acgcccgctg ctgctggtcg
     5461 acatcggcgt gccgagggac atcgaccaca cggttggtga gttgcccggc gtgacgtact
     5521 acgacctcga cgggctacag gccatcgctg ccgagcacta cgccgtccgc gccggcgagg
     5581 tcgcgacggt tcagaccatg gtcgacgagg aaacggaccg cttcgtcgag tggtgggagc
     5641 agctccaggt cgtgccgacc atcgccgcgc tcaccgaccg cgccgagcgg ctgcgctcca
     5701 cggagctagc aaagacgctg cgccggctga acgtgggtga cgaggaacgc gcgcacctcg
     5761 aggccctcac gcgagcgctc gtgaagcaga tcctgcacga ccctatcgcc accctgcgcg
     5821 agcgaggcga ccgcgaggtc tacatcgacg ccgtgcggaa gctcttccgg ctcgacgacg
     5881 aaccgggcga cctcgaaaac cgcgaactca gttgacccac ctccggctcg cgacgcgcgg
     5941 ctccgctctc gcgctcgccc agagcgagct ggtcgctgcc gccctgcgtg cggccgaccc
     6001 ggcggtcgag gtcgagttcg tcgaggtgcg caccgagggc gacgcggacc gccgcacacc
     6061 cctgagcatc ctcggcgggc gcggcgtctt cgtccgtgcg gtcgaggaag cgctcctcga
     6121 ggggcgcgcg gacatcgcta tgcactcgct caaagacgtg ccgaccacgg ttccggaggg
     6181 actcgtgctc ggggcgttcc tcgaacgcgg cgacccacgc gacgtagtcc tcgaagctcc
     6241 agcccgcgcg cctgaccagt cgtcggtagg gcatcgggtc catgagcagg gtggcctgcg
     6301 gcgccgcgag cttcgggccg ccgatgccgt ggagctcgcc gccggcgtac gtcttgtcga
     6361 ggaggaacag cgtgccaccg atcaccaccg tcgggtccag gcctgcgtgc gacagaatcg
     6421 tcgcca
//
LOCUS       DAORPG010000024         6407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_605_length_6407_cov_3.189232, whole genome shotgun sequence.
ACCESSION   DAORPG010000024 DAORPG010000000
VERSION     DAORPG010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_605_length_6407_cov_3.189232"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="PLX85_01105"
     CDS             complement(<1..999)
                     /locus_tag="PLX85_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRC61804.1"
                     /translation="MLGVMGKILWVDLTSGDIREERLPDEMYEQYVGGYGVAARLLYD
                     RMPANADPLGPDNVFGIVTGPLTGTSAVTGNRFTAIGKSPKTNTWGDANCGGTFGPSL
                     KFAGYDGVFVTGISERPVYLLIDNGVASLHDAADIWNQGDDLHPERVLRERYGKKFEV
                     AWIGRAGETMSLTAGVMNDIGRSAARSGLGAVMGSKKLKAIVAGGSQTIPLHDAEGLR
                     LYAAAAIKTTKAENSPTWQAFSEYGTTGGVAASAASGDSPVKNWAGVGIRDFTGAFAI
                     SDVALDEYKVKRTGCWHCNIACSAHMRVPEGKYAVETHRPEYETLAAAGTMTLSNDL"
     gene            complement(1073..2104)
                     /locus_tag="PLX85_01110"
     CDS             complement(1073..2104)
                     /locus_tag="PLX85_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC61805.1"
                     /translation="MTAPAEVVRLDGLRVHLGKRDILVVEEFVATAGEAVALLGPNGS
                     GKSTLLRTAALLIEPAAGSVHLFGARPRGRGERARLRRRTASVFTDPTLLDMSARGNV
                     EVALGIHGVSGSERRRRAERWLERLGVAHLAGARPHTLSAGEAQRIALARAFAVDPEL
                     LFLDEPFASLDFETRARLVGDLRELLAASEVTALIATHDRGEAELLADRLAVLVGGRL
                     VQDGPTRQVLEYPRSAEVASLLGHALLTPEQVRCLAPRVEARGVLAHLPPGAVRLADD
                     VEPGASRVPLLSIRGAGGRVQLVCDLGGPATFEVGAAHIAGRELRPGAMLALAVDIER
                     LHWLPDTTR"
     gene            complement(2101..2799)
                     /locus_tag="PLX85_01115"
     CDS             complement(2101..2799)
                     /locus_tag="PLX85_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC61806.1"
                     /translation="MDLIFDAFSEAWRLLRTGDGDVYGIAWRTLTITGSSTLISMLIG
                     VPIGLLLALGRFPGQRILVAFSYAGMGMPPVVAGLVVAILLWRSGPLGQLGLIYTPGA
                     MVIAQSLIATPVIVAVTSTGVMALPPALRLQIRAMGAGDARYLWLVAREARLPLLVAT
                     MAGFGAVVSEVGASTVVGGNLAGETRVLTTASVLEMSKGNFGTALALGLILLAIVFAI
                     AVVLAAALHRMRRA"
     gene            complement(2802..3704)
                     /locus_tag="PLX85_01120"
     CDS             complement(2802..3704)
                     /locus_tag="PLX85_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRC61807.1"
                     /translation="MRRSSIISLLAAALLAVLLLGACGDDDSAAEEQGTASATAAATA
                     SATPPAGGEELILATTTSTDNSGLLGVLIPLYERATGDHVKVIAVGSGAAMQMGERGD
                     ADVLLVHSPKAEETFMAAGNGESRKRVMYNDFIIVGTPEDKASIKGGKDAAAAMAAIA
                     RTESSFISRGDNSGTNAAEKDLWAAAGLKVPTGASWYTETGQGMEATLTITAERNGYT
                     LTDRATWLATADPKRLPLLVEGDAKLFNVYHVIVVNPAKHDGLHVKAARRFSDFLLAS
                     STQKTIGEFGKDKFGVSLFIPYPD"
     gene            3808..4179
                     /locus_tag="PLX85_01125"
     CDS             3808..4179
                     /locus_tag="PLX85_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRC61808.1"
                     /translation="MERDDGIVLMSEFRADLLEGVAAEGSVAAAARTLGLPNRTAWKK
                     LEEMERAAGVSLIDTSSGGSHGGGTTLTEAGRALVEAYRRISDPVSAEVQGRFEAERA
                     LFERETAPSPHRTPDRADASS"
     gene            4219..5502
                     /locus_tag="PLX85_01130"
     CDS             4219..5502
                     /locus_tag="PLX85_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRC61809.1"
                     /translation="MNAHLRDVRMRGFAERSDVEDVERFLRAHTAPLPAEAVALLDCV
                     GRVLAEDVVSTVNVPGFVRAAMDGYAVRGEDTFGASAYDTIALRVLGQSLPGNPFRGQ
                     VDAGGAVRIMTGAPLPDGADAVIMAEVCREAGGLVEVSEAVAPRRNVDEVGEDVARGQ
                     LLLRTGRRLRPQDAGLLASIGRGVLTCVARPRVQLLITGNELLPPGAAPEGPHIVDSN
                     SVVLSALVARDSGHLLPVCRLADDRERIRAALASSEADVILVSGGSSVGMEDFAPSLV
                     AELGTLDVHGISMRPSSPTGIGRLPGDGGRERLVFLLPGNPVSCMCAYEFFAGPAIRA
                     LGGRPWSWPHARVRLPLARKIVSQVGRTDYVRVAIEGGRAVPLATSGASILSSTVRAD
                     GAVIVERGLEGMPEGAEVEVLLYDEPGETPASRER"
     gene            5502..>6407
                     /locus_tag="PLX85_01135"
     CDS             5502..>6407
                     /locus_tag="PLX85_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HRC61810.1"
                     /translation="MVQQQFLEVLDRDEAEARWHAVIAPAALEPEVVPLADALGRVLA
                     EDVRAEVDVPGFDRSNMDGFAVRALDTYGAMEEKPVRLRLNAEVLPTGVIPAVEVVDG
                     SATTIATGGMLPRGADAVVPVEQTDIEDGGATVVARRPVVPGENISFAGTDIGAGETV
                     LFAGTRLTSRDTGVLAAIGAAEVAVVRRPRVAVISTGDEVAQPGAPMRPGLVFDSNGR
                     IIADAVRELGGDPWFLGSYPDDEDAIRGAVERALADADMVLLSGGTSKGEGDLNSRVV
                     GALTPGIVVHGVALKPGKPICLAAQG"
BASE COUNT          927 a         2267 c         2270 g          943 t
ORIGIN      
        1 gaggtcgttc gagagcgtca tcgtcccggc ggcggccagc gtctcgtact cgggacggtg
       61 cgtctcgacg gcgtacttgc cctcggggac ccgcatgtgc gcgctgcacg cgatgttgca
      121 gtgccagcag cccgtgcgct ttaccttgta ctcgtcgagg gcaacgtcgc tgatcgcgaa
      181 cgcgcccgtg aaatcgcgaa tgccgacgcc ggcccagttc ttcacgggcg agtcaccgga
      241 ggcggcgctc gcggccacgc cgccggtcgt gccgtactcg gagaacgcct gccacgtggg
      301 cgagttttcg gccttcgtcg tcttgatcgc cgcggcggcg tagagccgca ggccctcggc
      361 gtcgtgcagc gggatcgtct ggctgccccc ggcgacgatc gccttcagct tcttcgagcc
      421 catgaccgcg ccgaggcccg aacgcgcggc ggaacgcccg atgtcgttca taacgccagc
      481 cgtcagggac atcgtctcgc cggcgcgtcc gatccaggcg acctcgaact tcttgccgta
      541 gcgctcgcga aggacgcgct ccgggtggag gtcgtcgccc tggttccaga tgtcggcggc
      601 gtcgtgcagc gaagcgactc cgttatcgat gaggaggtac accgggcgct ccgagatccc
      661 tgtgacgaag acgccgtcgt agccggcgaa cttcagcgac ggcccgaagg tgccgccgca
      721 gttggcgtcg ccccaggtgt tcgtcttcgg cgacttgcca atcgccgtga accggttgcc
      781 ggtgaccgcg gacgtgcccg tgagcgggcc cgtcacgatg ccaaacacgt tgtccggacc
      841 gagcggatcg gcgtttgcgg gcatccggtc gtacagcagc cgggccgcca ccccgtaccc
      901 gccgacgtac tgctcgtaca tctcgtcggg caggcgctcc tcgcggatgt caccgctggt
      961 gaggtctacc cacaggatct tgcccatgac gccgagcatc tcgtcctcct cgtaacgtgc
     1021 gtttcacgct caagggtagc gaggcgtatc gaggagcgga acaaagcgat cgttatcgag
     1081 tcgtgtcggg cagccagtgc agccgctcga tatcgaccgc gagcgccagc atcgcccccg
     1141 gccgcagttc ccgccccgcg atgtgggcgg cgccgacctc gaaggtcgcg gggccgccga
     1201 ggtcgcacac aagctggacg cggcctcccg cgccccggat cgaaagcagc ggcacgcgtg
     1261 acgcgcccgg ctcgacgtca tccgcgaggc ggacggcgcc cggcggcagg tgcgcgagga
     1321 cgcctcgagc ctcgaccctg ggggcgaggc aacgcacttg ctccggcgtg agcagcgcgt
     1381 gcccgaggag actcgcgacc tctgcggacc gcgggtactc gagcacctgg cgcgtcgggc
     1441 catcctggac gagacgacct ccgacgagga ccgcgaggcg gtccgccagg agctccgcct
     1501 caccgcggtc gtgcgtggcg atgagcgccg tcacctccga ggcggcgagc agctcacgca
     1561 ggtcgccgac gagccgcgcc cgcgtctcga agtcgagcga tgcaaacggc tcatcgagga
     1621 atagcagctc cgggtccacg gcgaaggccc gcgcgagcgc gatgcgttgt gcctcgccgg
     1681 cggagagtgt gtgcggccgc gcgcccgcga ggtgggcgac ccccagccgc tccagccagc
     1741 gctccgccct acgccggcgc tcgctgccag acacgccatg gatgcccagc gcgacttcga
     1801 cgttcccacg cgcggacatg tcgaggagcg tcgggtcggt gaagacgctg gcggtgcggc
     1861 ggcgcaggcg agcgcgctcg ccgcgtcctc gtggtcgcgc accaaacaga tggacgctgc
     1921 cggccgccgg ctcgatcagc agcgcggccg tccggagcag cgtggacttg cccgacccgt
     1981 tcggcccgag cagcgcgacg gcctctcccg ccgttgccac gaactcctcg acgacgagga
     2041 tgtcccgctt cccgagatgc acgcgcaggc catcgaggcg gacaacctcg gccggcgccg
     2101 tcatgcgcgc cgcatacggt gcagcgccgc cgccaggacg acggcgatcg cgaagacgat
     2161 ggcgagcagg atgagcccga gcgcgagtgc cgtcccgaag ttgcccttgc tcatctcgag
     2221 gacggaggcc gtcgtgagca cgcgcgtctc gcccgccagg ttgccgccga cgacggtcga
     2281 agctcctacc tcggacacga ccgcgccaaa gccagccatc gtcgccacga ggagcggcag
     2341 gcgcgcctcg cgagccacga gccagaggta gcgcgcatcg ccggcgccca tggcccggat
     2401 ctggaggcgt aacgcgggcg gcagggccat cacgccggtg ctcgtcacgg ccacgatgac
     2461 gggtgtggcg atcaacgact gcgcgatcac catcgcgccg ggcgtgtaga tgagcccgag
     2521 ctgccccagt gggcccgacc gccagagcag gatcgcgacc acgaggccgg cgacgacggg
     2581 cggcatcccc atccccgcat acgagaacgc gacgaggatc cgctgccccg ggaaccgccc
     2641 gagcgcgagc aacagtccga tcggcacccc gatcagcatt gagatcagag tcgagctgcc
     2701 ggtgatcgtg agcgtccgcc aggcgatccc gtagacgtcg ccgtcaccgg tccgaaggag
     2761 ccgccacgcc tcgctgaacg cgtcgaagat caggtccacg gtcagtcagg gtaggggatg
     2821 aacagcgaga cgccgaactt gtccttcccg aactccccga tcgtcttctg cgtgctgctc
     2881 gcgaggagaa agtcgctgaa gcgacgggcg gccttcacat gcagcccgtc gtgcttcgcg
     2941 gggttcacga cgatcacgtg gtagacgttg aagagcttcg cgtcgccttc cacgaggagt
     3001 ggcaaccgct tcgggtcggc ggtcgccagc cacgtcgcgc ggtcggtcag cgtgtagccg
     3061 ttgcgctcgg cggtgattgt gagcgtcgcc tccatgccct ggccggtctc ggtgtaccag
     3121 gacgcgcccg tcggcacctt gaggccggcc gccgcccaca ggtccttctc ggccgcattc
     3181 gtgccggagt tgtctccccg gctgatgaac gaggactcag tccgtgcaat cgcggccatc
     3241 gccgcggcgg cgtccttgcc ccccttgatg cttgccttgt cctccggtgt cccgacgatg
     3301 atgaagtcgt tgtacatgac gcgtttgcgc gactcgccgt ttccggcggc catgaacgtc
     3361 tcttccgcct tcggcgagtg cacaaggagg acgtcggcgt ccccgcgttc ccccatctgc
     3421 atcgccgcgc ccgagcccac ggcgatcacc ttcacgtggt cgcccgtcgc gcgctcgtac
     3481 agcgggatca acacgcccag gagaccggaa ttgtcggtgc tggtggtcgt cgcgaggatc
     3541 agctcctcgc cgccggccgg cggcgtggcg gacgcggtgg ccgccgccgt cgccgacgcg
     3601 gtaccttgct cctcggcggc ggagtcgtcg tcgccgcagg ctccgaggag caggaccgcc
     3661 agcagagcgg cggcgagcag actgatgatg gacgaacgtc gcacgagttc cccctccgag
     3721 cgcattgcac gacggggcga cacgctacca gagccggcgc ccacgcgaca ggggcggcct
     3781 ggtctgcaca ttcgcacgaa ggtgtgggtg gagcgcgacg acggcatcgt cctgatgagc
     3841 gagttccgcg ccgacctcct cgaaggcgtg gcggccgagg gatccgtggc cgcggctgcg
     3901 aggacgctcg gcctcccgaa ccgcaccgcg tggaagaagc tcgaagagat ggaacgggcc
     3961 gccggtgtgt cgctcatcga tacgtcttcc ggcggatccc acggcggtgg cacgacgctc
     4021 accgaggcgg gccgcgcgct tgtggaggcc taccgccgga tcagcgaccc ggtctcggcc
     4081 gaggtacagg ggcgcttcga ggccgagcgc gcgctgttcg agcgggagac ggcgccctcc
     4141 ccgcatcgga cgccggatcg cgccgacgcg tcatcgtaag atcggctgcg tgccgcactc
     4201 accagaggcc cagcgacgat gaacgctcac ctgcgtgacg tgcgcatgcg tggcttcgcc
     4261 gagcgctccg acgtcgagga cgtggagcga ttcctccgcg cgcacacggc gccgctcccg
     4321 gcggaggccg tcgcgctcct cgattgcgtc ggtcgcgtgc tcgccgagga cgtcgtttcg
     4381 acggtcaatg tgccgggctt cgtgcgcgcc gcgatggacg gctacgccgt gcgcggcgag
     4441 gacacgttcg gcgccagcgc atacgacacg atcgcgctgc gcgtcctcgg ccaatcgctg
     4501 cccggcaacc cgttccgagg tcaggtcgac gctggcggag cggtacgcat catgaccggc
     4561 gcacccctgc ccgacggtgc ggacgcggtg atcatggccg aggtctgccg cgaagccggg
     4621 ggcctggtcg aggtcagcga ggccgtggcg cctcgccgca acgtcgacga ggtcggtgag
     4681 gatgttgccc gcggccagtt gctgctgcgt accgggcggc gacttcgccc ccaggacgcc
     4741 ggcctgcttg cttcgatcgg ccggggagtt ctgacctgcg tagcgcggcc gcgcgtgcag
     4801 ctgctgatca ctggaaacga gttgctgcct cccggagccg cgcccgaggg cccgcacatc
     4861 gtcgacagca actcggtcgt gctgagtgcg ctcgtcgcgc gtgattctgg gcacctgctc
     4921 ccggtatgcc gcctcgcgga cgaccgcgaa cggatccggg ccgcgctcgc ctcgtccgag
     4981 gcagacgtca tcctcgtgtc cggtggcagc tccgtgggca tggaggactt tgcgccgtcg
     5041 ctggtcgcgg agctcggcac gctcgacgtg catggcatct cgatgcggcc gtcgagcccg
     5101 accggaatcg gccggctccc cggcgacggt gggcgcgagc gcctcgtgtt cctgctcccc
     5161 ggcaacccgg tgagctgcat gtgcgcctac gagtttttcg ccggccccgc gattcgtgcg
     5221 ctcggcggcc ggccctggag ctggccgcac gcgcgcgtac gcctgcccct cgcccgcaag
     5281 atcgtctcgc aggtaggccg caccgactac gttcgcgtgg cgatcgaggg gggtcgagcc
     5341 gtgccgctgg cgacctcggg cgcttcgatc ctctcgtcga ccgtccgtgc ggacggagcg
     5401 gtgatcgtgg agcgcggcct cgaagggatg ccggaggggg cggaggtcga ggtgctgctg
     5461 tacgacgagc cgggcgaaac gcccgcgtcc cgggaacgct gatggtgcag cagcagttcc
     5521 tcgaggtgct cgaccgcgac gaggcagaag cacgctggca cgcggtgatc gcgcccgcgg
     5581 ccctcgaacc cgaagtcgtg ccgctcgcgg acgcgctcgg gcgtgtgctc gccgaagacg
     5641 tgcgcgccga ggtggacgtg cccgggttcg accgctcaaa catggacggc ttcgcggtcc
     5701 gcgcgctcga tacgtatggg gcgatggagg agaagcccgt ccgcctgcgc ctgaacgccg
     5761 aggtgctgcc caccggcgtc atcccggccg tcgaggtggt cgacggctct gcgacgacca
     5821 tcgccaccgg cggcatgttg cctcgcggcg cggacgcggt cgtcccggtt gagcagaccg
     5881 acatcgagga cggcggcgcg acggtcgtcg cgcgccgccc ggtcgtgccc ggggagaaca
     5941 tctcctttgc cggcacggac atcggcgcgg gcgagacagt gctcttcgcc gggacgcgcc
     6001 tcacctcgag ggacacgggc gtcctcgccg cgatcggggc cgctgaggtg gccgtcgtac
     6061 gccggccgcg cgtcgccgtc atctccaccg gcgacgaggt cgcgcagccc ggcgccccga
     6121 tgcgccccgg cctcgtgttc gacagcaacg gccggatcat cgccgacgcc gtacgtgagc
     6181 tcggcggcga tccctggttc ctcgggagct acccggacga cgaggacgcc atccgcggcg
     6241 ccgtcgaacg cgccctcgcc gacgccgaca tggtgctgct ctcaggcggg acctcgaagg
     6301 gtgaggggga cctcaacagc cgcgtcgtcg gtgcgctgac gcccggcatc gtcgtgcacg
     6361 gggtcgcgct caagccgggc aagccgatct gcctcgcggc gcaaggc
//
LOCUS       DAORPG010000025         6355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_612_length_6355_cov_3.568095, whole genome shotgun sequence.
ACCESSION   DAORPG010000025 DAORPG010000000
VERSION     DAORPG010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_612_length_6355_cov_3.568095"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1040)
                     /locus_tag="PLX85_01140"
     CDS             complement(72..1040)
                     /locus_tag="PLX85_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC61811.1"
                     /translation="MYRFFSHRFGAASAALLALALATLLLVACGDDDGNSEAAGASSG
                     GSGTATFPRTITDSSNTKVTIPAQPKRIISLSPAATEILFAVGAGDRVVGSDKFSNYP
                     EAALKTAKLDYSKPDPEAALALNPDLVIMATRQEEQVKQFRDLKMTVLYFEEAATVDG
                     VYDSITLLGQLTGHDDQAAQLVTSMRQRVTAMTSKVADVTKGPRVFYELTTDLYTASP
                     DTFIGSMLSLLKAQNVAAGAASEFPQLTAEAVIAADPEVILLADAEFQAQNADTVLTR
                     PGWANVSAVKNRRVFGIDDDLASRPGPRIVEAMEIVAKALYPDRFR"
     regulatory      complement(1178..1318)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     regulatory      1514..1637
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            1748..1912
                     /locus_tag="PLX85_01145"
     CDS             1748..1912
                     /locus_tag="PLX85_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61812.1"
                     /translation="MIVEDDWYGKFGTCIACGYVHETQVADPNEILEEERLAAGKQRR
                     RQPSHGKLRL"
     gene            2091..2372
                     /locus_tag="PLX85_01150"
     CDS             2091..2372
                     /locus_tag="PLX85_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61813.1"
                     /translation="MRPKHGREDEVNGLIDRLVSYYEQQPGYITGYRLRNIDGTGRVG
                     RLGVWDRHEDAEHAATTDHDLALRSQLNLIIEDESHEEYSFDGIPTNPG"
     gene            2711..4408
                     /gene="ilvB"
                     /locus_tag="PLX85_01155"
     CDS             2711..4408
                     /gene="ilvB"
                     /locus_tag="PLX85_01155"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRC61814.1"
                     /translation="MRMSGADIVLESLKRLGIDTIFGYPGGVVLPLYDRFPAHPEVRH
                     ILVRHEQGGGHAADGYARAKGALGCALATSGPGATNLVTAITNAMMDSVPTLFITGNV
                     ARNLLGKDGFQEADITGITMPVVKHNYLVMRAEDIAYTFAEAAYIATTNRPGPVHIDI
                     PKDVFTEEADFIWPESVSIRGYDAFPPLDEAMLRQAASYIDGAERPIIIAGHGVILSS
                     AWDELKTFAEKTGIPVLNTLLGLGSISTDHALAYGMLGMHGSYEANHAASNADVIIGI
                     GNRMDDRALGRFADFNPTAKIVHIDIDPAEHGKNIETHAAIVGDAKQALAALTSMVRQ
                     NTHVEWLRWIDDLRAEHSFSMAVPSGPELSVQHVLKRISEVNDDSVVIVTGVGQHQMW
                     AAQHLKFTHPHQLITSGGLGTMGFEVPAALGAQVARPDATVWSICGDGGFQMTLQELA
                     TMVDENLPVKMAIMNNGYLGMVRQWQELFYADNYVSVAMSQPDFVKLAEAYGILGIRV
                     ESKDQVDDAIRTANAHPGPVILDFRVEAEGNVWPMVPAGAALKETVEAPERPQEVAR"
     gene            4444..5025
                     /gene="ilvN"
                     /locus_tag="PLX85_01160"
     CDS             4444..5025
                     /gene="ilvN"
                     /locus_tag="PLX85_01160"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRC61815.1"
                     /translation="MKHTVIALVEDRPGVLNRCASLFRRRGFNIDSLAVGTTEEPGIS
                     RMTIVVEDEGAPGIVEQVEKQLYKLIDVIDVANLTSAEAVMRELALVKVRCTSAQRRE
                     VLDLIDIFRGHVVDVSPAHLTAEIVAAEDRMTAFIENLRPYGILELVRTGRIAMMRGP
                     QTNVVHELEPDSVLRFHTESGRRPEGELPYVSD"
     gene            5306..>6355
                     /locus_tag="PLX85_01165"
     CDS             5306..>6355
                     /locus_tag="PLX85_01165"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRC61816.1"
                     /translation="MAEKVYIFDTTLRDGEQSAGVGFTIEDKVEIARQLAKMNVDIIE
                     AGFPFSSPGDLEAVSLIAREVKGPVIAGLARANFDDVDACWQAVQRAETPRIHVFISA
                     SEIHMAHALRKNREDVVEMARAAVHRAAGYTSDVEFSPMDATRSDPQFVFDILQAAVE
                     EGATTINIPDTVGYAIPQEFERLVRSIRERLPDHVRISVHCHNDLGMAVANSLAGVLG
                     GARQVEGAINGIGERAGNTSIEEVIMAIKTRGDFLNMYTDVNTREIYQASRLVERISG
                     MMLQPHKAIVGKNAFRHSSGIHQDGVLKLRETFEIMDPADIGVPVGDSIVLTKVSGRH
                     GLRARIAALGVNLEAD"
BASE COUNT         1078 a         2215 c         2112 g          950 t
ORIGIN      
        1 ggcctgcgcc ggccgaggcc ccgaggggcc gcgcctgcgg cgcctcgacg ctcacgcgac
       61 ccgcctcgag gtcaccggaa gcggtccggg tacaacgcct tcgccacgat ctccatcgcc
      121 tccacgatgc gcgggcccgg ccggctcgca aggtcatcgt cgataccgaa gacgcggcgg
      181 ttctttacgg cggagacgtt cgcccagccc ggccgcgtca acaccgtgtc cgcgttctgc
      241 gcctggaact cggcatcggc gagcaggatg acctcggggt cggcggcgat cacggcctcc
      301 gccgtgagct gcgggaactc cgaggctgcc cccgccgcga cgttttgcgc cttgagcagg
      361 ctcagcatcg aaccgatgaa cgtgtcgggg gaggccgtgt acaagtcggt cgtcagctcg
      421 tagaagacgc gcggcccctt cgtgacgtcg gcgaccttcg aggtcatcgc cgttacccgc
      481 tgccgcatgc tcgtcacaag ctgcgccgcc tggtcgtcgt gcccggtcag ctgccccagg
      541 agcgtgatgc tgtcgtagac gccgtcgacg gtcgcggctt cctcgaagta gaggacggtc
      601 atcttcaggt cgcggaactg cttgacctgc tcctcctgcc gcgtggccat gatcacgagg
      661 tcggggttca gcgcgagcgc tgcttccggg tccggcttcg agtagtccag cttcgccgtc
      721 ttgagcgcgg cctcggggta gttcgagaac ttgtcgctgc cgaccacccg gtcgcccgcc
      781 ccgacggcga acaggatctc cgtcgccgcc ggcgacagcg agatgatgcg cttgggttgc
      841 gcgggaatcg tgaccttcgt gttcgaggag tccgtgatgg tgcgcgggaa cgtcgccgta
      901 cccgaaccgc ccgaggacgc cccggccgcc tcggagttgc catcgtcgtc gccgcaggcg
      961 acaagcaaca gggtcgccag cgcgagcgcg agcagcgccg ccgatgccgc cccgaatcga
     1021 tgtgagaaga aacggtacaa gtgaacctcc gcacactcgt gcgagaggag gcctcgggct
     1081 cccgaacaca caacgccccc tcgaggtgag ggggcgggct tcgggtacgt ggtgcgcgtt
     1141 ggaagcgtgc gagccacgtg ccgtcacccc gtcctcgcgg gtggacacat gcgcaggccg
     1201 gtactctggc tcgccgggtc gaggcctggg cctcggtcgg ctcacagttg cgggacagcg
     1261 ccgggcttgc accggacttc cccggtctct gcgcgagacg cgaggagcat aagaagggtg
     1321 gccggccggc gcaactcgga atcgccgccg ccgcgggcag gcgaggcgag atcgatgtgc
     1381 ctcgagatgt aaaatttccg tcaagattgg gttgacaagg caaatcgcct gagtatgctc
     1441 ttgttgtcac aatcacgaag ttatcgtgta cgtgatgaac aacacgaagt tgctggcgcc
     1501 gggcgcggcg ccctgccaaa ttctcgcggg agcgagaacc gggggaacca cctttctggg
     1561 gtgaacttca ggcgtacgcc tcgcgcctga gcgggcccac ctggtcggcc cgaacccgtc
     1621 agctaacccc ggcggcagca ggaactaggc cctatccgga gggctcccgg gcgcacccca
     1681 cccatcgagg gggcacgccg tgatcgttac caccccgccc aagcagtgtc cgcgttgccg
     1741 tggcctcatg atcgttgagg acgactggta cgggaaattc ggcacctgca tcgcctgtgg
     1801 ctatgtccac gaaacccagg tcgccgaccc gaacgagatc ctcgaggagg agcggctggc
     1861 cgccggcaag cagcgtcgcc gccagccctc gcacggcaag ctccgcctgt aggcgagggt
     1921 ccggcgcttc gagactcgcg catcctcggc cggcgtctac acgcgccggc tcgttctcgc
     1981 tacactgcgc tgacccgcag ccgggcacgc gtcgtgatcg cgtcggcggg gaatgccact
     2041 gctcagctga tgaagggtgg gtgccgatgc catacgtcag gatttccctc atgcggccga
     2101 agcacggccg tgaggacgag gtgaacggcc tcatcgaccg cctcgtctcc tactacgagc
     2161 agcagcccgg ttacatcact ggctaccgcc tgcgcaacat cgacggtacg ggccgcgtcg
     2221 gccgcctcgg cgtgtgggac cgccatgagg acgccgagca cgctgcgacc acagaccacg
     2281 acctggcgct ccgctcgcag ctcaacctca tcatcgagga cgagtcgcac gaggagtact
     2341 cgttcgacgg catcccgacc aacccgggct agcccggctg cgcggccaca acgaacgagg
     2401 gctcccggtg agccctcgtt tcgatctccc cccaatcatc tggcgcatag tccgccgccc
     2461 cggttgacgc gggcggtctg ccgcaacgat actgataggc gttatgccga gcctcggaga
     2521 ccgcaccgca tcctcgctgg ccgtccaggg ctcacggacg tcgcgtcgcg acggccgcct
     2581 cgcactcgta ctcgtactta cctaggcggc gcctgcgctt cgtcgcagcc gtcgccgtcc
     2641 gccagacccg cactcgcggg cctgattcgt gccacccagc ccatcgagta gtgaacagga
     2701 ccccggcacc atgagaatga gcggcgcaga catcgtcctc gagagcctga agcgcctcgg
     2761 gatcgacacg atcttcggct accccggcgg cgtcgtattg ccgctctacg accgcttccc
     2821 ggcgcacccg gaggtgcggc acatcctcgt gcggcacgag cagggcggcg ggcacgccgc
     2881 cgacggctac gcccgggcga aaggtgcgct gggctgcgcg ctggcgacgt ccggtcccgg
     2941 cgccacgaac ctggtcacgg cgatcaccaa cgcgatgatg gactccgtcc ccaccctgtt
     3001 catcacgggc aacgtcgcga ggaacctgct cggcaaggac ggcttccagg aggccgacat
     3061 caccggcatc acgatgcccg tagtcaagca caactacctc gtcatgcgcg ccgaggacat
     3121 cgcgtacacc ttcgccgagg ccgcgtacat cgccacgacg aaccggccag gcccggtgca
     3181 catcgacatc ccgaaggatg tcttcaccga ggaggccgat ttcatctggc cggagtccgt
     3241 ctcgatccgc ggctacgacg ccttcccgcc cctcgacgag gcgatgctgc ggcaggcggc
     3301 gagctacatc gacggcgcgg agcggccgat catcatcgcg gggcacgggg tcatcctgtc
     3361 gagcgcctgg gacgagctga agacgttcgc cgagaagacc ggcatcccgg tcctgaacac
     3421 gctgctcggc ctcgggagca tctccaccga tcacgccctg gcgtacggca tgctcggcat
     3481 gcacggctcc tacgaggcga accacgccgc ctcgaacgcg gacgtgatca tcggcatcgg
     3541 caaccgcatg gacgaccgcg ccctcggccg cttcgccgac ttcaacccga ccgcgaagat
     3601 cgtgcacatc gacatcgacc cggccgagca cggaaagaac atcgagacgc atgccgcgat
     3661 cgtcggcgac gcgaagcagg ccctcgccgc actgacctcg atggtgcgcc agaacactca
     3721 cgtcgagtgg ctgcgctgga tcgacgacct gcgcgccgag cactccttct cgatggcggt
     3781 gccctccggc cccgagctgt cggtgcagca cgtcctgaag cggatctccg aggtgaatga
     3841 cgacagcgtg gtcatcgtca cgggcgtggg ccagcaccag atgtgggcgg cccagcacct
     3901 caagttcacg cacccgcacc agctgatcac ctcgggcggc ctcggcacga tgggcttcga
     3961 ggtgccggcg gccctcgggg cgcaggtggc gcgcccggac gcgacggtgt ggtcgatctg
     4021 cggcgacggc ggcttccaga tgacgctgca ggagctcgcc acgatggtgg acgagaacct
     4081 gcccgtgaag atggcgatca tgaacaacgg ctacctcggc atggtccgcc agtggcagga
     4141 gctgttctac gccgacaact acgtgtcggt cgcgatgtcg cagccggact tcgtgaagct
     4201 ggccgaggcg tacggcatcc tcggcatccg cgtcgagtcg aaggaccagg tggacgacgc
     4261 gatccgtacg gcgaacgcgc acccggggcc ggtcatcctc gacttccgcg tcgaggccga
     4321 aggcaacgtc tggccgatgg tgcccgcggg cgccgcgttg aaagaaaccg tcgaggcccc
     4381 ggagcggccg caggaggtcg cccgctaggg cgggacaagc tcggaccgag gaatgaggac
     4441 ggcatgaaac acaccgtgat cgcgctggtg gaagaccggc ccggcgtgct caaccgctgc
     4501 gcatcgctgt tccggcggcg cggcttcaac atcgactcgc tcgcggtcgg cacgaccgag
     4561 gagcccggca tctcgcgcat gaccatcgtc gtcgaggatg aaggcgcgcc gggcatcgtc
     4621 gagcaggtcg agaagcagct ctacaagctg atcgacgtga tcgacgtggc gaacctgacg
     4681 agcgccgagg ccgtgatgcg cgagctcgcg ctggtgaagg tgcgctgcac ctccgcgcag
     4741 cgccgcgaag tgctcgacct catcgacatc ttccgcggcc acgtcgtcga cgtgtcgcct
     4801 gcgcacctga cggcggagat cgtcgccgcc gaggaccgca tgacggcctt catcgagaac
     4861 ctgcgcccct acggcatcct cgaactggtg cgtacgggcc ggatcgcgat gatgcgagga
     4921 ccgcagacga acgtcgtcca cgagctggag ccggactccg tgctccgctt ccacaccgag
     4981 tcgggacgcc gccccgaggg cgagctgccc tacgtcagcg actagcaccg ggcacgagga
     5041 cggccgtacg tgagcactga gcgcgccacc gggatcgagg gttccatgac ctcggtgggt
     5101 cgcgcctcgc gtcgcgcggc ggtagccgta cgcccctcgt tggccggcag tgcatcgcga
     5161 gcggacgccg aagggcgtcc gctccgtcta tccgtgcgca gccggctcgg cctcgcgctt
     5221 ccccggccct ccgccgccac ctcgatggcg gacgggaggg cacagcggcg acgaccgatg
     5281 accaggacga aacgaggaat ctgacatggc cgagaaggtc tacatctttg acaccaccct
     5341 gcgcgacggc gagcagtccg ccggcgtggg tttcaccatc gaggacaagg tcgagatcgc
     5401 ccgccagctg gcgaagatga acgtcgacat catcgaggcg ggctttccgt tctcctcgcc
     5461 cggcgacctc gaggcggtct cgctgatcgc ccgcgaggtg aagggcccgg tcatcgccgg
     5521 gctggcgcgg gcgaacttcg acgacgtcga tgcctgctgg caggccgtgc agcgagccga
     5581 gacgccgcgc attcacgtct tcatctcggc gagcgagatc catatggcgc acgccctgcg
     5641 caagaatcgc gaggacgtcg tcgagatggc ccgcgccgcc gtgcaccgcg ccgccggcta
     5701 cacctcggac gtcgagttct cgccgatgga cgccacgcga tcggacccgc agttcgtctt
     5761 cgacatcctg caggccgccg tcgaggaggg cgcaacgacg atcaacatcc ccgacaccgt
     5821 cggctacgcg atcccgcagg agttcgagcg cctcgtgcgc tcgatccgtg agcggctgcc
     5881 ggatcacgtc cgtatttcgg tgcactgcca caacgacctc gggatggccg tggcgaactc
     5941 actggcgggc gtcctcggcg gcgcgcgcca ggtggagggc gccatcaacg gcatcggcga
     6001 gcgtgccggc aacacctcca tcgaggaggt gatcatggcg atcaagacgc gcggcgactt
     6061 cctgaacatg tacacggacg tcaacacccg cgagatctac caggcgagcc gcctcgtcga
     6121 gcgcatctcc ggcatgatgc tgcagccgca caaggcgatc gtcggcaaga acgccttccg
     6181 ccactcctcg ggcatccacc aggacggggt gctcaagctg cgcgagacgt tcgagatcat
     6241 ggaccccgcc gacatcggcg tgcccgtcgg cgactccatc gtgctgacga aggtgtccgg
     6301 ccgccacggg ctgcgcgcgc gcatcgcggc actgggcgtg aacctcgagg cggac
//
LOCUS       DAORPG010000026         6303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_625_length_6303_cov_3.885083, whole genome shotgun sequence.
ACCESSION   DAORPG010000026 DAORPG010000000
VERSION     DAORPG010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_625_length_6303_cov_3.885083"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..619
                     /locus_tag="PLX85_01170"
     CDS             158..619
                     /locus_tag="PLX85_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRC61817.1"
                     /translation="MARRTPPKHIRTLALAVLIHRDHLLCARGYDEVKQQTFYRPLGG
                     GVDFGERASEAVVRELREEIGREVEASELLAVTENIFTFNGGAGHEVCFEYLARFVAG
                     SEPADLEPIDCDEHGRSFTACWLPLAEVLGGMHVVYPDGLRERLAEWVNRL"
     gene            837..2828
                     /locus_tag="PLX85_01175"
     CDS             837..2828
                     /locus_tag="PLX85_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRC61818.1"
                     /translation="MKARYTLAAVAVASAFALMALSAGGRDADAQTPTAPAGDYIVVL
                     KDGVPDVFGIINQIERLTGVQTKLEFSTALKGFSAHLTGPQVVALQQLPWVQFLSPDY
                     QVQAVGSVALQPGDSAPTGVRRIEAATTSTARQASSVGVAVIDTGIDLTHPDLNAVAG
                     KNCVSTSSQPQDDNGHGTHVSGTIGAKNTGSGVVGVAPGTTLYAVKVLNSSGSGTWAQ
                     VICGIDWVTANAQTLNIKVASMSLGGGGSNDNNCGNSNNDALHKAICNSVNAGVTYVV
                     AAGNSGANLSGFTPAAYPEVLAVTAMSDSDGQAGGTGGAPTCRTGESDDRYASFSNYA
                     VSSADINHTIAGPGTCIYSTWVSGSTHTYNTISGTSMATPHVSGSVALCLGEAGGSGP
                     CSGKTPAQVIQLMRSNAQTRSQTPGYGFTGDPNAPVNGRYYGYLVANTTPPPPTPDFS
                     VAASPSSRSVQPGQGTTFTVTVTGVGGYVTPVNLTVGGLPANANASFSPAQVTPSGTG
                     VTSTLNITTAGNTPLSTSTLTITGTAQSGGLTHSTTVSLTVANTPPPDFTLSVTPSSR
                     TVSRPIFSFFAATTTYTVTLTSVSGYSLPVTLSVSGLSSGITGSFNSNPRTPTAGGVT
                     STLTLSILRSATPGTRTLTITGVGSDNNTKTATVSLTVN"
     gene            complement(2853..5189)
                     /locus_tag="PLX85_01180"
     CDS             complement(2853..5189)
                     /locus_tag="PLX85_01180"
                     /inference="COORDINATES: protein motif:HMM:NF019935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRC61819.1"
                     /translation="MASRPAAPALERFLEARTAEAVRASAGLGVEASRDLTDRLDEAI
                     CQLAEPVARDGLALVALGSYGRRELCRHSDVDIMLLVRGETSEAVNAVLYPLWDAGLK
                     VGHSVRTPDQAIESARQNVETLTSLLDARLICGDARLYEGFLAARRRLVRAERARMRT
                     DLAERRRALIEREPWQLQEPDVKTSRGGLRELHVIHWLTLADAIVEGAEAKPLGPDLA
                     AAQETLLSARNALHALTDRPNDRFRLDLARPVAELLGLSRTECGRRLFNAMRLVDAGI
                     EAALRSEVSAPRGLLKRVFTRRDDARPTTTSVEDDASDLDRLLAALRVPPAGLDPLPR
                     APWLERILPEWEVLRALPHVAPFHLHPVDVHAMRTVAEARRAAAEDTEETATTVAAEE
                     FGNPDELLLAAALHDIGKGHEGDHSEVGAVIAERFAARVGLDADAAHRLMLAAQHHLL
                     LPTVATRRDIADERVIREVADAIGDVPTLHLLYVIGVADARASGPDVWNPWKATLMRS
                     LYLRVLDRLSQTGSEDVSVSELRRHATVEALQGQFGIDRVRHHLDQLPANYVLSASPE
                     TIGTHLELIERAAGGTALDHDREGDLDRLTIVTPDRPGILSLVAGTLAVHNVNVHGGS
                     AYTRDDGVAIEVMYVSDGLGHGIDERRWARVRQDVPLALAGDFPLDERLAETRQAYRR
                     EPPAPIETAVHVDNVGSDRYSIVEVNAADRLGLLYAITHALHGMSLDIHLAKVDTVGH
                     EVVDAFYVLRENGRRVAAPDEIARLQRRIIEAVAALDR"
     gene            complement(5191..5529)
                     /locus_tag="PLX85_01185"
     CDS             complement(5191..5529)
                     /locus_tag="PLX85_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRC61820.1"
                     /translation="MKMIVAYINPYKLDEIRDALKDAGVSGLSVSNIQGFGRQAGHTE
                     TYRGAEYEVDFVPKVRIEVLVDETLAPTVISTIETTARTGSIGDGKIAVLPVEDVIRI
                     RTGERGRDAL"
     gene            complement(5560..>6303)
                     /locus_tag="PLX85_01190"
     CDS             complement(5560..>6303)
                     /locus_tag="PLX85_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HRC61821.1"
                     /translation="GGTVVHINAGAAALAMVFVLGPRLGFRREIIRPHSLPLTLLGAG
                     ILWFGWFGFNAGSAFGANGLAANAFVVTQIAAGVAGTVWALMEQIRHGKATTLGFASG
                     AVAGLVAITPASGFVGPGGAVAIGAAAGVICFLALQIKFAFNFDDALDVVAVHLVGGI
                     LGSLLVGVFADAELNPASKDGLFFGGGVDQLGRQAAAVGIALVYSFVVTYVLGRILDA
                     VMGLRVAEQDERRGLDLALHEEQSYVLAD"
BASE COUNT         1016 a         1981 c         2341 g          965 t
ORIGIN      
        1 gggatttcgc cgagtgcgta gatctccttt tcggcggcct gaaccacggt cactgctccc
       61 atcgtgcgtt gcctgctgct gctgctgttg tctggccacg acgggcccac gtccgcgtat
      121 gctaccacca actcccccga ccggaacgga gccctccgtg gcgcgccgaa cgccgcccaa
      181 gcacatccgc acgctcgcgc tcgcggtgct gatccatcgg gatcacctgc tctgcgcgcg
      241 cggatatgac gaggtgaagc agcagacgtt ctaccggccg ctggggggcg gtgtggactt
      301 cggcgagcgc gcctcggagg cggtggtgcg ggagctgcgg gaggagatcg gccgcgaggt
      361 agaggccagt gaactgctgg cggtgacaga gaacatcttc acgttcaacg gcggcgcggg
      421 gcacgaggtt tgcttcgagt acctcgcgcg gtttgtcgcc ggttcggagc cggcagacct
      481 cgagccgatc gattgcgatg agcacgggcg gtcgttcacc gcgtgctggc tgccactggc
      541 ggaggtgctc ggggggatgc acgtggtgta tccggacggg ctgcgcgagc gcctcgcgga
      601 gtgggtgaat cggctgtagg gctgccgtgt aagcgggttt ggactggtag gccgagcctc
      661 gtgcgccatg ctcagcgggg gacgactgtc ggcgggccac gccctcgatg cccttgccgt
      721 gtcatcggga cgcgcgagca gcggctgttg ccgctggctc ctgctggtat cctgtgctgt
      781 acgtcttttc cgagcgtaac ggtgatgaaa cgcggcgggc ggagatcggc aggtctatga
      841 aagcaaggta cacgctggcg gcggtcgccg tggcctcggc gttcgcgctc atggcattgt
      901 ccgcgggagg ccgagacgcg gacgcacaga cgcctaccgc gcccgcgggc gactacatcg
      961 tggtgctgaa ggacggcgtg ccggacgtct tcggaatcat caatcagatc gagcggctca
     1021 cgggcgtcca gacgaagctc gagttttcga ccgcgttgaa agggttctcc gcgcatctga
     1081 ccggtccgca ggtggtcgcc ctccagcagc tgccctgggt gcagtttctg agcccggact
     1141 atcaggtgca ggcggtcgga tcggtggccc tgcaaccggg ggatagcgcg ccgaccgggg
     1201 tgcgccgcat cgaggcggcg acgacgagca cggcccgtca ggcgagcagc gtgggcgtgg
     1261 ctgtcatcga cacgggcatc gacctgactc acccggacct caacgccgtg gccggcaaga
     1321 actgtgtttc cacctcgagc cagccgcagg acgacaacgg tcacgggacg cacgtgtccg
     1381 ggacgatcgg tgcgaagaac accgggagtg gcgtcgtggg cgtggctccc gggacgacgc
     1441 tgtacgcggt aaaggtgctg aactcctcgg ggagcggcac gtgggcgcag gtcatctgcg
     1501 gcatcgactg ggtcacggcg aacgcccaga cgctcaacat caaggtcgcg agcatgagcc
     1561 tcggcggagg cggatcgaac gacaacaact gcggcaactc aaacaacgac gcgctgcaca
     1621 aggcgatctg caactccgtc aacgcgggcg tcacctacgt ggtggcggcc gggaacagcg
     1681 gagcgaacct gtccggcttc acgcccgccg cctatccgga ggtccttgcg gtcaccgcaa
     1741 tgagtgacag cgacgggcag gccggtggta cgggtggcgc cccaacctgc cggaccggag
     1801 agagcgacga ccggtacgcg agcttctcga attacgcggt atcgagcgcc gacatcaacc
     1861 acacgatcgc cgggccggga acgtgcatct actcaacctg ggtgagcggc tccacgcaca
     1921 cgtacaacac catctcgggc acgagtatgg cgacgccgca cgtgtcggga tcggtggcgt
     1981 tgtgcctcgg tgaggccggg ggcagcggcc cctgcagcgg caagacgccg gcgcaggtga
     2041 tccagttgat gcgctccaac gcgcagactc gctcgcagac gccgggatac gggttcaccg
     2101 gtgaccccaa cgctccggtg aacgggcgct actacgggta cctggtggcg aacaccacgc
     2161 ctccgcctcc gactccggac ttcagcgtcg ccgcctcgcc ctcgtctcgc agtgtgcagc
     2221 cggggcaggg gacgacgttc acggtgacgg tcacgggcgt cggtggatat gtgacgccag
     2281 tgaatctgac ggtcggtgga ctgccggcga acgcgaacgc cagcttcagc ccggcgcagg
     2341 tgacgccgtc ggggacgggt gtgacctcga cgctgaatat cacgacggcg gggaacactc
     2401 cgctgtcgac ctcgacgctc acgattacgg gtaccgcgca aagcgggggg ctgacgcaca
     2461 gcacgacggt ctcattgacc gttgcgaaca cgccgccgcc ggacttcacg ctgtcggtca
     2521 cgccatcgtc gcggacggtg tcgcggccga tcttctcgtt ctttgcggcg acgacaacct
     2581 acacggtgac gctgacgtcg gtgagcgggt actcgctgcc tgtgacgctg tcggtcagcg
     2641 ggctgagcag cggcatcacg gggagcttca actcgaaccc gcggacgccg acagcaggcg
     2701 gagtgacctc gacgttgact ctgtcgatcc tgcgcagcgc gacccctggc acgaggacac
     2761 tgacgatcac cggggtcggg agcgacaaca acacgaagac ggcgacggtg tcgctgacgg
     2821 tgaactagcg gacggttcga ggcggtggcg gcctagcgat cgagggccgc taccgcctcg
     2881 atgatgcggc gctggaggcg cgcgatctcg tccggggcgg ccactcgccg cccgttctcg
     2941 cgcaggacgt agaaggcgtc gaccacctcg tggccgacgg tgtccacctt cgcgaggtgg
     3001 atgtcgagcg acatgccgtg cagtgcgtgg gtgatcgcgt agagcaggcc gaggcggtcg
     3061 gctgcattca cctcgacgat tgagtagcgg tcggacccca cgttgtcgac gtgcacggcg
     3121 gtttcgatcg gagcgggcgg ctcgcggcga taggcttggc gcgtctcggc gagacgctcg
     3181 tcgagcggga agtcgccggc gagagcgagc gggacatcct ggcggacacg cgcccagcgg
     3241 cgctcgtcga tgccgtggcc gaggccgtcg ctgacgtaca tcacctcgat cgcgacgccg
     3301 tcatcgcggg tataggcgct gccgccgtgg acgttgacgt tgtgtaccgc gagcgtgccg
     3361 gccaccagcg agaggatgcc ggggcggtcg ggcgtgacga tggtgaggcg gtcgaggtcg
     3421 ccctcgcggt cgtggtcgag agcggtgccg ccagccgcgc gttcgatgag ctcaaggtgg
     3481 gtcccgatcg tctccggtga ggcgctgagc acgtagttcg ccgggagctg gtcgaggtgg
     3541 tgacgaacgc ggtcgatgcc gaactggccc tggagcgcct cgacggtggc gtgccggcgt
     3601 agctccgaga cgctgacgtc ctcggacccg gtctgtgaaa gccggtcgag gacgcgcagg
     3661 taaagcgacc gcatcagcgt ggctttccag gggttccaca cgtccggtcc gctggctcgg
     3721 gcgtcggcga cgccgatcac gtagaggagg tggagcgtcg ggacgtcccc gatcgcatcg
     3781 gctacctctc ggatgacgcg ctcgtcagcg atgtcgcgcc gggtcgcgac cgttggcagc
     3841 agcaggtggt gctgcgcggc gagcatgagg cggtgggcgg cgtcggcatc gagtccgaca
     3901 cgggcagcga agcgctcggc gatgacggca ccgacctcgc tgtgatcgcc ctcgtggccc
     3961 ttaccgatgt cgtgcagggc ggcggccaga aggagctcgt ccggattgcc gaattcctcg
     4021 gcggccaccg tggtcgcggt ttcttcggtg tcctcagctg ccgcgcggcg cgcttcggcg
     4081 accgtccgca tggcgtgcac atcgacaggg tggaggtgaa acggcgcgac gtggggcagg
     4141 gcgcgcagaa cctcccactc ggggaggatg cgttcgagcc agggcgcgcg tggcagggga
     4201 tcgagcccgg cgggcgggac gcgaagggcg gcgagcagcc gatcgaggtc cgaggcgtcg
     4261 tcttccacgg atgtcgttgt gggtcgagca tcgtccctgc gcgtgaatac ccgcttcagc
     4321 agaccccgcg gagcgctcac ctcggatcgg agggctgcct cgattccggc gtccaccagg
     4381 cgcatcgcgt tgaacaggcg acgcccacac tcggtgcgtg agaggccgag cagctcggcg
     4441 acggggcggg cgaggtcgag ccgaaagcga tcgttgggcc ggtcagtgag ggcgtgcagg
     4501 gcgtttcgcg cgctgagcag cgtctcctgc gcagcggcca gatctggacc gagcggcttc
     4561 gcttcggcac cttcgacgat ggcgtcggcg agggtgagcc agtggatgac gtggagctcg
     4621 cggaggccgc cgcggctggt cttcacgtcc ggctcctgga gctgccatgg ttcgcgctcg
     4681 atgagggcgc gacggcgctc agcgaggtcg gtgcgcatgc gggcgcgttc ggcgcgcacg
     4741 agcctgcgac gggcggcaag gaacccctcg tacagccgag cgtcaccgca gatgagccgg
     4801 gcgtcgagaa gtgaagtgag cgtctccacg ttctggcgtg ccgactcgat ggcctgatca
     4861 ggagtacgca ccgagtggcc gaccttgagg ccggcatccc agagcgggta gaggacggcg
     4921 tttaccgcct cggaggtctc gccgcggacc aggagcatga tgtccacgtc ggaatggcgg
     4981 cagagctctc ggcggccata gctgccgagt gcgacgagcg cgagtccatc gcgggcgacg
     5041 ggctcggcga gctggcagat ggcttcatcg aggcgatcgg tgagatcgcg gctcgcctcg
     5101 acgccgagtc cggcggaggc gcgcacggct tctgccgtac gcgcctccag aaagcgctcg
     5161 agcgcggggg ccgccgggcg gctggccacg ttagagggcg tcccgccctc gctcgccggt
     5221 gcggatgcgg atgacatcct cgaccggcag gaccgcgatc ttgccgtcgc cgatggagcc
     5281 ggtgcgcgcc gtggtctcga tggtggagat cacggtgggg gcgagcgtct catcgacgag
     5341 cacctcgatg cggactttgg gcacgaagtc gacttcgtac tcggcgccgc ggtaggtctc
     5401 ggtgtggccg gcctggcgac cgaagccctg aatgttgctg acggagaggc cgctcacgcc
     5461 ggcgtccttg agggcgtctc ggatctcgtc gagcttgtag gggttgatat aggcgacgat
     5521 cattttcatg cgggaatccc tctcttcgcc tcggatgcct cagtcggcca ggacgtatga
     5581 ctgctcctcg tggagcgcga ggtcgaggcc gcggcgctcg tcctgctcag ccacacgcag
     5641 gcccatgacg gcatcaagga tacggccgag gacgtacgtg accacgaagg agtagacgag
     5701 cgcgatgccg accgcggcgg cctgacggcc gagctggtcg acaccgccgc cgaagaacag
     5761 tccgtccttg gacgcggggt tgagctcggc gtcggcgaag acgccgacga ggagcgagcc
     5821 gaggatgccg ccgacgaggt gcaccgccac aacatcgagc gcgtcgtcga agttgaaggc
     5881 gaacttgatc tggagcgcga ggaagcagat cacgccggca gccgcaccga tggcgaccgc
     5941 accgccgggg ccgacgaagc cggaggccgg cgtgatggcg accaggccgg cgaccgcgcc
     6001 agaggcgaag cccagcgtcg tcgccttgcc gtgacggatc tgctccatga gcgcccagac
     6061 ggtgcccgcg acgccggccg cgatctgggt aacgacgaag gcgttcgcgg cgaggccgtt
     6121 ggcgccaaag gcagagccgg cgttgaaccc gaaccagccg aaccagagga tgcccgcgcc
     6181 caggagcgtc agcggcagcg agtggggccg gatgatctcg cgacggaagc cgagcctcgg
     6241 gccgaggacg aagaccatgg cgagggcggc ggctccggcg ttgatgtgga cgacggtgcc
     6301 gcc
//
LOCUS       DAORPG010000027         6246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_636_length_6246_cov_3.439186, whole genome shotgun sequence.
ACCESSION   DAORPG010000027 DAORPG010000000
VERSION     DAORPG010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_636_length_6246_cov_3.439186"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1744
                     /locus_tag="PLX85_01195"
     CDS             <1..1744
                     /locus_tag="PLX85_01195"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRC61822.1"
                     /translation="VIIKIADRLHNMRTLQHLAPHRQQAIALETMDIYAPLAARLGIW
                     QFKWELEDLAFRYLEPEDYRRISEMVASRRAERERFVRRVERELRQQLETAGIQAEVS
                     GRVKHLYSIRSKMRRYAAEGKSFDQIYDLLALRVLVDTVGDCYNALGVVHQTWHPIPG
                     GFDDYIANPKESLYQSLHTNVMGPGARPFEVQIRTHEMHELAEYGVAAHWQYKDEPRK
                     RDHQYEERLAWLRHLIEWQQEASGTEDFLESVKTDVFRDQVFVYTPRGDVRVLPAGST
                     PIDFAYRIHTDLGHHCVGARVNGRIAPLTTRLRNGDVVEVIRGRGDKGPSRDWLIPAL
                     GYLGSSHSRQKVRHWFRRSQRDENIDRGRDLLDRERRRLGVGELPASLASQLGYESLE
                     DMLAAIGYGDVSLQLLAMRLAEYAPPPPEPLQTITKKPRKAGTPGVRVLGEGGMHVQV
                     ARCCHPLPGELIIGFITRSRGVTVHRRDCRNVSHAAEPERLVECDWGPSGSLYSAAVH
                     VHAWDRVGLLRDISTIVASEDVNMVGVRTVENDDRTTTVHLTLETQGLPQVSRLMGRL
                     ESVRGVISVSRGEV"
     gene            1821..2687
                     /locus_tag="PLX85_01200"
     CDS             1821..2687
                     /locus_tag="PLX85_01200"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HRC61823.1"
                     /translation="MTTIQPIGAHVSSSGGIWTAVERGAALEAEAIQIFGSAPQTWRK
                     TNHTPAAFERFRLARAESSLRAVWLHCPYLPNLAHEDDEMWEKSIDCVVNALTVADGA
                     GADGVVLHTGSHRGKGIDAVLPRVAAAIERIMEAAPGNALLALENAAGQGGTIGKSFE
                     ELGAIFRAVATPRLAVCLDTCHAYAGGYDIAHPEGMQAMLEEADREVGLDRLAVIHAN
                     DSKMEFGSTRDRHENIGDGHIGSEGFRNILATPELQRLPMLLEVPGLDGKGPDFENVR
                     RLKQLRDEVRTT"
     gene            complement(2692..3003)
                     /locus_tag="PLX85_01205"
     CDS             complement(2692..3003)
                     /locus_tag="PLX85_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61824.1"
                     /translation="MPIDERLAAEGADWIAEQLTEEYGGFISAEVIDAIMEFELEIRE
                     ATGDPLLPHAVMATHLLEKLAAEGAPVGVWGGVNEVLLQEILHLEDEFLAMAGHPRKI
                     R"
     gene            complement(3097..3927)
                     /gene="thyX"
                     /locus_tag="PLX85_01210"
     CDS             complement(3097..3927)
                     /gene="thyX"
                     /locus_tag="PLX85_01210"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRC61825.1"
                     /translation="MPIEVRLTDHTTDPIRTLYTAFRVCYSAFTPAQIEARIEDERIT
                     HDQMREFVEARLATGHTSPLQQVWFEFGISGVSRAFSHQFVRHHMGISFEQQSQRYVT
                     YKDGSFPYTVPDSIRRAGKSEAFEQAIETAGRAYQELIDAGVPAEDARFLLPNATSTN
                     FKVSVNYLELLHIADQRLCTRAQWEFRKVVSLMRAEVKRRFPELARYMQPKCGEFRLG
                     YCDESYADWEACPIGRKRPHKTTLFELYARSQQGQLDDLRDNDYQVIESEQLSESEPY
                     "
     gene            4082..4261
                     /locus_tag="PLX85_01215"
     CDS             4082..4261
                     /locus_tag="PLX85_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61826.1"
                     /translation="MLLIKACPRCQGDVTPIVDFEGDLQLSCVQCGYSKYVPRGRRPD
                     LAEAKPVVGTLRRAS"
     gene            complement(4262..5506)
                     /locus_tag="PLX85_01220"
     CDS             complement(4262..5506)
                     /locus_tag="PLX85_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015123554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HRC61827.1"
                     /translation="MARTIHRVVVTGMGAVTPIGNDLASYWEGLRTGRNGIHRVTLCD
                     PDPLPVQIAGEVKGWDPEQYLERKTARRTGRFAQFAHAASKQALEDGGVVVDGSNRDE
                     IGVVMATSGGAFNMGEPWMEMQAKGAHRADPFVITREGQHMGAGRVGRELGIRGPNTT
                     INTACASGTDALGHALNMIRLGHTRYLLAGGAEAIITPLALASMGRMGALSHNNGDPE
                     HASRPFDLNRDGFVLGEGAGVLLLETEESALARGARIYCELAGVGWSFDATDDTAPDA
                     EGQSLAMTRALKDAELEPTDVDYINCHGTSTPYNDRTETVAIKLALGEAHAKSIPVSS
                     TKSMTGHLAAGAGGIEAVAAILTLHHGCIPPTINYVTPDPACDLDVVPNVARQASLNV
                     VMSNSFGLGGQNAAAIFRRYES"
     gene            complement(5538..>6246)
                     /locus_tag="PLX85_01225"
     CDS             complement(5538..>6246)
                     /locus_tag="PLX85_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC61828.1"
                     /translation="YRVIAIDQRGHGRSDRAGDRDYSRASQVEDIAAVIEALGLRMVT
                     LVGHGMGGANAICYAAEHPDVVSALIVIEAAPEVLRSGIETMRRVLASGDEFSTLDEV
                     SEVFRRYYPYATTEQLDRRARAAVTVTADGSYTWAFDPVFRDPTARPPEQDPGQRRLS
                     NLWDCVEHVQCPVMIVSGSETDMVTPEAIQRLHRRILGSRRSLIEEAGHAVPTDQPAA
                     LAQHIREFLQSLSSSPI"
BASE COUNT          993 a         2150 c         2049 g         1054 t
ORIGIN      
        1 cgtgatcatc aagatcgccg accgcctgca caacatgcgc acgctgcagc acctcgcgcc
       61 gcatcgccaa caggcgatcg cgctcgaaac gatggacatc tacgcgcccc tcgctgcgcg
      121 cctcggcatc tggcagttca aatgggagct cgaggacctc gcgttccgct acctcgaacc
      181 cgaggactac cggcgcatct ctgagatggt ggcgtcgcgt cgggccgagc gtgaacgctt
      241 cgtacgccga gtggagcgcg agctccgtca gcagctcgag accgcgggca tccaggctga
      301 ggtcagcggc cgggtgaagc acctctattc gatccgctcg aagatgcggc gctacgcagc
      361 cgaagggaag tcgttcgacc agatctacga cttgctcgcc ctccgcgtcc tcgtcgacac
      421 cgtgggggac tgctacaacg cgcttggggt ggtgcatcag acatggcatc ccatacctgg
      481 cgggtttgac gactacatcg cgaacccgaa ggagtcgctg taccagtcgc tgcacaccaa
      541 cgtgatgggg cccggcgcgc gccccttcga ggtacagatc cgcacccacg agatgcacga
      601 actcgccgag tacggcgtcg ccgctcactg gcagtacaaa gacgaacccc ggaagcgcga
      661 tcaccagtac gaagagcgcc tcgcctggct gcgccacctc atcgagtggc agcaggaagc
      721 ctctggcacc gaggacttcc tcgagtctgt gaagaccgac gtgtttcgag accaggtctt
      781 cgtgtacacc cctcggggcg acgtgcgggt gcttcccgcc ggctcgaccc cgatcgactt
      841 cgcctaccgc atccacactg acctcggcca ccactgcgtg ggcgcgcggg tgaacgggcg
      901 tatcgccccc ctcacgaccc gcctccgcaa cggcgacgtt gtcgaggtga tccggggccg
      961 cggggataag gggccatcgc gcgactggct gatcccggcc ctcggctatc tcggctccag
     1021 ccactcccga cagaaggtgc ggcactggtt ccgccgctcg cagcgcgacg agaacatcga
     1081 tcgcggtcgc gacctcctcg accgcgaacg caggcgcctg ggtgtggggg agctgccggc
     1141 gtcgctggcc tcgcaactcg gctacgagtc gctcgaggac atgctcgccg ccatcgggta
     1201 cggcgacgtc tcgttgcagc tgctcgcgat gcggctcgcg gagtacgctc ctccgccgcc
     1261 tgagccgctg cagacgatca cgaagaagcc ccgcaaggca ggcacgcccg gcgtgcgggt
     1321 cctgggcgaa ggcggcatgc acgtccaggt cgcgcgctgc tgccacccac tccccggcga
     1381 gctcatcatc gggttcatca ctcgctcacg aggtgtgacg gtgcatcggc gcgactgccg
     1441 caatgtgtct cacgcagcgg agcccgagcg tctcgtcgag tgcgactggg gtccgtccgg
     1501 cagcctctat tccgccgccg tgcatgtcca cgcctgggat cgcgtcggac tgttgcgtga
     1561 catcagcacg atcgtggcgt ccgaggacgt gaacatggtc ggggtccgta ccgtcgagaa
     1621 cgatgaccgc acgacaacgg tccacctcac gctcgaaacg caggggctgc cccaggtctc
     1681 gcggctcatg ggccgcctcg agtccgtccg cggggtaatc tcggtcagtc gcggcgaggt
     1741 ctgagcctgc cgtcggcctc tcgtctccgg cctgctacga tgcagcctcg ccactccccg
     1801 cgcgccccgg aaggacgggt atgacaacga tccagccgat cggcgcccac gtctcttcgt
     1861 cgggtggcat ctggacggcc gtcgaacgcg gggcggccct tgaggccgag gcgatccaga
     1921 tcttcggcag tgcgccgcaa acctggcgca agacgaacca cactccggcg gcgttcgagc
     1981 gctttcggct cgcccgcgcc gagtcctcgc tgcgtgccgt gtggctgcat tgtccgtacc
     2041 tccccaacct cgcccacgag gacgatgaga tgtgggagaa gtccatcgac tgcgtcgtca
     2101 acgcgctgac ggtcgccgac ggcgcgggcg cggatggcgt cgtactccat accgggtcgc
     2161 accgaggcaa aggcatcgac gccgtactcc cacgggtcgc cgcggctatc gagcgcatca
     2221 tggaggctgc ccccgggaac gccctgctcg cgctcgagaa cgcggctggc cagggtggca
     2281 cgatcgggaa gagtttcgag gaactgggcg ccatcttccg agcggtcgcc acgcctcgac
     2341 tcgccgtctg tctcgacacc tgccacgcct atgccggtgg ctacgacatc gcgcacccgg
     2401 aggggatgca ggcgatgctc gaggaagcag accgcgaagt cggactcgac cgcctcgcgg
     2461 tgatccacgc caacgactca aagatggagt tcggcagcac gcgcgaccgc cacgagaaca
     2521 tcggcgacgg ccatatcggc agcgaggggt ttcgcaacat cctcgccacg ccggagctgc
     2581 agcggctgcc gatgctcctc gaggtcccgg gtctcgacgg gaaagggccg gacttcgaga
     2641 acgtgcgccg cctcaagcag ctccgcgacg aggtgcggac gacctaggcc gttagcggat
     2701 cttccgcggg tgtcccgcca tcgccaggaa ctcgtcctcc agatgcagga tttcttgtag
     2761 cagcacctcg ttcacgccgc cccagacgcc gaccggtgcg ccttcagccg ccagcttctc
     2821 gaggaggtgc gtggccatca ccgcatgcgg gagcagtggg tcgcccgtcg cctcgcggat
     2881 ctcgagttcg aattccatga tcgcgtcgat cacttcggcg gagatgaagc ccccgtactc
     2941 ctcggtgagc tgctccgcga tccagtcggc gccctcagcg gcgagacgct cgtcgattgg
     3001 catgcttccc ccctcgatgc tccgaaacat ggtcacgaga ctgtggaggc cacgatattc
     3061 gaccgcccga ggccgccgca aggctcagcg cgagggtcag tacggctcgc tttcgctgag
     3121 ctgctccgac tcgatgacct ggtagtcgtt gtcccgcaaa tcgtcgagct ggccctgctg
     3181 cgaacgggcg tacagctcaa acagcgtggt cttgtgcggt cgcttgcggc cgattgggca
     3241 tgcctcccag tccgcgtacg actcgtcgca ataccccagg cggaactctc cgcactttgg
     3301 ttgcatgtac cgcgccagct ccgggaagcg tcgcttcact tccgcgcgca tgagcgagac
     3361 gaccttgcgg aactcccact gcgcccgcgt gcagagccgc tgatcagcga tgtgcagcag
     3421 ctcgaggtag ttcacgctca ctttgaagtt ggtgctcgtg gcgttgggaa gcaggaaccg
     3481 ggcgtcctcg gcgggcacgc ccgcgtcgat cagctcctgg taggcccgcc ccgccgtctc
     3541 gatcgcctgc tcgaatgcct ctgatttccc ggcccgccga atggagtcag ggacggtgta
     3601 cgggaacgag ccgtccttgt acgtgacata acgctgtgac tgctgctcga acgagatgcc
     3661 catgtggtgc cgcacgaact gatgcgagaa cgctcggctc acgcccgaga tcccaaactc
     3721 gaaccacacc tgctgcagcg gcgaggtgtg ccccgtcgca aggcgtgcct cgacgaactc
     3781 ccgcatctgg tcgtgggtga tgcgttcgtc ctcgatgcgc gcctcgatct gggcaggggt
     3841 gaacgcggag tagcacaccc ggaacgccgt gtagagcgtg cggatgggat cagtcgtgtg
     3901 gtcggtcagt cggacttcga tcggcattgg gcctcggcaa acgggggagt tcacccattg
     3961 tacgagcgtg cgcggcggcc gtccgagtgc gcgacgggtt ttacatcgag gcaacacctc
     4021 gtcaacagaa gcgtcacgag ttctcgcgat gatcgtccca ggcgacgaat ggaggaattc
     4081 gatgctgttg atcaaggcct gcccccgctg ccagggcgac gtcaccccga tcgtggactt
     4141 cgagggcgac ctgcagctct cctgtgtgca gtgtggctac tcgaagtacg tgccgcgtgg
     4201 ccggcgcccg gatctcgccg aggcaaagcc ggtcgtcggc acgctgcgcc gagcgtcctg
     4261 actacgactc gtagcggcgg aagatcgcgg ccgcgttctg cccgccgagc ccgaatgagt
     4321 tcgacatgac gacgttgagc gacgcttgcc gcgcgacgtt cggcacgaca tcgaggtcac
     4381 atgctgggtc cggggtgacg tagttgatgg tgggcggaat gcagccgtgg tgcagagtga
     4441 ggatggctgc gaccgcctcg atgccgcctg cccccgcggc gaggtggccc gtcatcgact
     4501 tcgtcgagga gaccgggatg ctcttcgcgt gcgcctcacc cagcgcgagc ttgatcgcga
     4561 cggtttcggt gcggtcgttg tacggggtgc tcgttccgtg gcagttgatg tagtcgacgt
     4621 cagtcggctc cagctcggcg tccttcaacg cacgggtcat cgccagcgac tgcccctcgg
     4681 cgtcgggcgc cgtgtcatcc gtcgcgtcga aggaccagcc caccccggcc agctcacagt
     4741 agatccgcgc tccccgcgca agcgcggact cctcggtctc cagcagcagc acacccgcgc
     4801 cctcaccgag gacgaacccg tcccgattca ggtcgaatgg ccgcgaggcg tgctcggggt
     4861 cgccgttgtt atgcgacagc gctcccatgc ggcccattga ggcgagcgcg agtggggtga
     4921 tgatcgcctc ggcgcctccc gcgagcaggt accgcgtgtg ccccaggcgg atcatattga
     4981 gcgcgtgccc gagcgcgtcg gttcccgagg cgcaggccgt gttgatcgtg gtgttcgggc
     5041 cgcggatgcc cagctcgcgg ccgacccggc ccgcccccat gtgctggcct tctcgcgtga
     5101 tcacgaatgg gtcggcccgg tgcgcgccct tcgcctgcat ctccatccat ggctcgccca
     5161 tgttgaaggc gccgcctgac gtggccatca ccacgccgat ctcgtcgcgg ttcgagccgt
     5221 caaccacgac cccgccgtcc tcgagcgctt gcttcgaagc cgcatgtgcg aactgggcga
     5281 agcggccggt gcgtcgagcc gtcttccgct cgagatactg ctccggatcc cagcccttca
     5341 cttcgcccgc gatctggacc ggtagggggt ccgggtcaca cagcgtgacc cggtgaatcc
     5401 cgttgcggcc cgtgcgtagc ccctcccagt acgaggcgag gtcgttgccg atcggcgtga
     5461 cggcgcccat cccggtcacc actacgcgat ggatcgtgcg ggccatgtga cggctccttg
     5521 gcaggcgacg cccgggacta aatcgggctg ctggacaggc tctgcaggaa ctcgcggata
     5581 tgctgtgcga gcgctgccgg ctgatcggtg ggcaccgcgt gcccggcctc ctcgatcagg
     5641 gagcggcgcg atcccagtat gcgacggtgc agccgctgga tcgcctccgg agtcaccatg
     5701 tcggtttcgg acccgctcac gatcatgacc ggacactgca cgtgctcgac gcagtcccac
     5761 aggttcgaga gccgccgctg gcccggatcc tgctcgggcg gacgggcggt cgggtcgcgg
     5821 aacaccgggt cgaacgccca ggtgtagctg ccgtcggccg tcaccgtcac ggccgcgcgt
     5881 gcccgacggt cgagctgctc ggtggtcgcg tacgggtagt agcgccggaa cacctccgac
     5941 acctcgtcga gggtgctgaa ctcgtctcct gaggcgagca cgcgacgcat ggtctcgatg
     6001 ccgctgcgca ggacttccgg cgcggcctcg atcacgatca gcgcgctcac gacgtccggg
     6061 tgttctgccg cgtagcagat ggcgttagcg ccgcccatcc cgtgcccgac cagggtcacc
     6121 atgcggagcc cgagcgcctc gatcaccgca gcgatgtcct cgacctgcga ggctcggctg
     6181 tagtcgcggt ccccggcgcg gtcagatcgc ccgtgccccc gctggtcgat cgcgatcacc
     6241 cggtat
//
LOCUS       DAORPG010000028         6198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_644_length_6198_cov_3.505616, whole genome shotgun sequence.
ACCESSION   DAORPG010000028 DAORPG010000000
VERSION     DAORPG010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_644_length_6198_cov_3.505616"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="PLX85_01230"
     CDS             complement(<1..465)
                     /locus_tag="PLX85_01230"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HRC61829.1"
                     /translation="MAVSRRLWAGVGILTAILVTGTVWYWLVEDFGFLDGMFMAVTTI
                     TTVGYREVHPLDTSGRLFTMVYLLGGVGVVFYTAVALVEAAVAGDIAEALGGARLSRK
                     VGRMQDHIVLCGFGRVGEEIARQLRGRKGSPQLVVIDKDPARREPALALGGLA"
     gene            complement(486..2762)
                     /locus_tag="PLX85_01235"
     CDS             complement(486..2762)
                     /locus_tag="PLX85_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRC61830.1"
                     /translation="MVLVHRYSEWDGTQEIPDLDASDVLEALSDDLMNFGDLQHALRN
                     LMQRGMQRQQGGRMQGLRDLLHQLRQQRRERLDQFDLGGVMDDLKRQLEEILDLERQA
                     LDERLGRQQSEDGQPGQDGPGAEAPSADGGQSGEDGEQQGQSGGQDGPRAGQQPGGQS
                     SQQGMRGQRSPGQRGQSGQGGPSGETGQPGEAGDSGEPAEGGSSDQQQFEEMLRRIAE
                     RKQQFLEGLPEDTAGQVKELQNYEFLNPEAQHKFQELMQQLKQAMTESFFKDVSQMVE
                     QMSAGDLQRMKDMVQALNDMLVQKMRGQEPDFDSFMQQYGDMFGPNPPQSLDELIEQM
                     QAQMNAMQSLMESLPSDQRQQLQSLLADKLGDPELEGELSQLALNMEYLNPTGERPNS
                     YNFRGDEEIDLQAAMNLMGEMQDIDELERSLERAQYEGDLDGVDAEKLRELLGDEAGE
                     TLDELQKLLDVLEEAGYIRRDGDNWELTPAGTRMIGQKALGEIYQQLKRQGIGNHAVP
                     ESGRHGERIDETKPYEFGDPFHLHMTRTIMNAVEREGPQAPVRLLPDDFEIYRSELMT
                     QTTTVVMVDLSWSMALRGSFQAAKKVALALHNLISAQYARDNLYVIGFSAYARELKVS
                     ELPYVRWDESVLGTNMHHALMLARKLLAKHRGGTRQVIMISDGEPTAHLERGRSQFSY
                     PPSPVTIRETLKEVKRCTQEGITINTFMLDRNYYLKEFVNQLAKINGGRVFYTTPEKL
                     GEYILVDYVQHKRKRVGR"
     gene            complement(2794..4194)
                     /locus_tag="PLX85_01240"
     CDS             complement(2794..4194)
                     /locus_tag="PLX85_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC61831.1"
                     /translation="MANEQPATLGALRASGYRVLPVREEIRKNLIANLEQGVTLFPGI
                     TGYEDTVIPQLENAILSGHDIILLGERGQAKSRIIRSLTSLLDEWTPVIAGVEIPENP
                     FAPISEQGRSLIEAMGDDTPIAWVHRDERYGEKLATPDITIADLIGEVDPIKVAEGRY
                     LSDQLTIHFGLIPRTNRGIFAINELPDLAERIQVGLLNAMEERDVQIRGHKVRLPLDV
                     FIVATANPEDYTNRGRIITPLKDRYGSQIRTHYPRNVEDEVSIIDQEAAQLSQEGYQV
                     VVPDYMKEVVAEITRLARRSPDINQRSGVSVRASVADYEAMVANAFRRAVRLGEREVA
                     PRISDLQFIKPALEGKVEFETVEDGREDQIVDRLIQGAVVAVFNRRCSITDLEPVIAA
                     FKSGISAETGEALPVSHYQQLLKQVDGLSPAVRQVTSDASPAAQAAAVELVLEGLHLN
                     KRLNKDSVIGQSRYRG"
     gene            complement(4393..4743)
                     /locus_tag="PLX85_01245"
     CDS             complement(4393..4743)
                     /locus_tag="PLX85_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC61832.1"
                     /translation="MTTNLEAAERERRRATGQRLRQWRMKRERSQAEVARTAGITQAS
                     LSNYETGKRELPLSTALSVAAALDISLGDVLDVSDIIVLRDSRLGKVVKTLVDRPDLL
                     DSIAPRRVESEAAS"
     gene            complement(4903..5739)
                     /locus_tag="PLX85_01250"
     CDS             complement(4903..5739)
                     /locus_tag="PLX85_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61833.1"
                     /translation="MRWGHLLTPWRLLRHSLFIAGLALTIAVAAVVTGVVLQGNDGTT
                     YPKTSASSSRDHGESTSIGALTRWVKSPGSVKAASADGGPESAATTATAEPEATAGAS
                     EGASAEEGAASEARVSTALTNEVPMLAERPADQGDMVVSAARFKVKSTPTVSEATTRT
                     QVTAPTAQDMRPGDELTVPVTFYYCEDSTGGQRRGDGGGFCGLMRDGTAVYPGAAACD
                     VVYLGQRFRIEGDPTGRTYVCADTGSAIHGQHRDIWFMSSGEGWAWQQVVGRSAVIQV
                     LP"
     gene            complement(5857..>6198)
                     /locus_tag="PLX85_01255"
     CDS             complement(5857..>6198)
                     /locus_tag="PLX85_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61834.1"
                     /translation="ADAATARLVRGLNSLFGELSVPGSAWAVSSMWHLNLGYEAPRPT
                     DVEWDASEEPQGVPERLVAPLKWALFNHGVDLMNTGGMVSSAHGDEEIDQTVEAFRAA
                     VGDLRAAGLLD"
BASE COUNT          883 a         2321 c         1853 g         1141 t
ORIGIN      
        1 cgcgagccct ccgagtgcga gcgccggctc tcggcgcgcc ggatccttgt cgatcaccac
       61 gagttgcgga ctgcccttcc gtccccggag ctggcgcgcg atctcctccc ccacccggcc
      121 gaacccgcac agcacgatgt ggtcctgcat ccgacccacc ttccggctga gccgcgcgcc
      181 cccgagcgcc tcggcgatgt cgcccgccac cgcggcctcg accagtgcca cggcggtata
      241 gaacacgacc ccgactcccc cgaggaggta caccatcgta aacagccgcc cgctcgtatc
      301 gagcgggtgc acctcgcggt accccaccgt cgtgatcgtg gtcaccgcca tgaacatccc
      361 gtcgaggaac ccgaagtcct cgacgagcca gtaccacacc gtaccggtga cgagaatggc
      421 ggtcaggatc ccgacgccgg cccacagccg gcggctcacc gccatcgact agtcccgtgc
      481 tgcaactacc gcccgacgcg cttccgtttg tgctgcacgt agtcgacgag gatgtactcg
      541 ccgagcttct caggcgtcgt gtagaacacg cgcccgccgt tgatcttcgc cagctggttc
      601 acgaactcct tcagatagta gttccggtcc agcatgaagg tgttgatggt gatcccctcc
      661 tgggtgcacc gcttcacctc tttgagcgtc tcccggatcg tcaccgggct cggcgggtac
      721 gagaactgtg atcgcccccg ttcgaggtgc gccgtcggct ccccgtccga gatcatgatc
      781 acctgccgcg tcccgcctcg atgcttcgcg agcaacttgc gggcgagcat caacgcgtgg
      841 tgcatgttcg tcccgagcac ggactcgtcc cagcgcacgt acggtagctc ggacaccttc
      901 agctcccgcg cataggccga gaacccgatc acgtacaggt tgtcccgcgc gtactgcgcg
      961 ctgatcaggt tgtgcagcgc cagcgcgacc ttcttcgccg cctggaacga tccccgcagc
     1021 gccatcgacc acgacaggtc gaccatcacc acggtcgtcg tctgcgtcat tagttccgag
     1081 cggtagatct cgaagtcgtc gggcagcagc cgcaccggcg cctgcggccc ttcacgttcg
     1141 acggcgttca tgatcgtgcg ggtcatgtgg aggtggaacg ggtcgccgaa ctcgtacggc
     1201 ttggtctcat cgatccgctc gccatgccgc cccgactccg ggaccgcgtg gtttccgatg
     1261 ccctgccgct tcagctgctg atagatctcg cccagcgcct tctgcccgat catccgcgtc
     1321 ccggccggcg tcagttccca gttgtcgccg tcgcgccgga tgtaccccgc ctcctcgagc
     1381 acatcgagga gcttctgcaa ctcgtcgagc gtctcgccgg cctcgtcgcc gagcagctcg
     1441 cgcagcttct ccgcgtcgac cccgtcaagg tcgccctcgt actgcgcccg ctccagcgag
     1501 cgctcgagct cgtcgatgtc ctgcatctcg cccatgaggt tcatggccgc ctgcaggtcg
     1561 atctcctcgt cgccccggaa gttgtacgag ttcggccgct ccccggttgg gttgaggtac
     1621 tccatgttga gggcgagctg tgacagctcc ccttccagct ctgggtctcc cagcttgtcc
     1681 gcgagcagcg actgcagctg ttgccgctgg tccgagggca acgactccat cagcgactgc
     1741 atcgcgttca tctgcgcctg catctgctcg atcagctcat cgagggactg cggcgggttc
     1801 ggcccgaaca tgtccccgta ctgctgcatg aaggagtcga agtccggctc ctgcccgcgc
     1861 atcttctgca cgagcatgtc gttcagcgcc tgcaccatgt ccttcatgcg ctgcaggtcg
     1921 ccggccgaca tctgctcgac catctgcgag acgtccttga agaaggactc cgtcatcgcc
     1981 tgcttcagct gctgcatcag ctcctggaac ttgtgctgcg cttcggggtt caggaactcg
     2041 tagttctgca gctccttcac ctggcccgcc gtgtcctccg gcagcccttc gaggaattgc
     2101 tgcttccgct ccgcgatccg ccgcagcatc tcctcgaatt gctgctggtc gctcgacccg
     2161 ccctcggcgg gctctccgct gtcaccggct tcgcccggtt gtccggtctc acccgacggc
     2221 ccaccctgcc cggactgtcc gcgctgcccc ggactccgct ggccgcgcat gccctgctgc
     2281 gaggactgac cgcccggctg ctgacccgcg cgaggcccgt cctggcctcc gctctggcct
     2341 tgctgctcac cgtcctcgcc cgactggccg ccatccgccg agggcgcctc cgcgccaggg
     2401 ccgtcctggc ccggctgccc gtcctcgctc tgctgccgcc cgagccgttc gtccagcgcc
     2461 tgccgctcga ggtcgaggat ctcctccagc tggcgcttga ggtcgtccat caccccgccg
     2521 aggtcgaact ggtccagccg ctcgcgccgc tgctggcgca actggtgcag caggtcgcgc
     2581 agcccttgca tgcgcccgcc ttgctggcgc tgcatcccgc gctgcatcag gttccgcagc
     2641 gcgtgctgca ggtccccgaa attcatcagg tcgtccgaca gcgcctcgag cacgtcgctc
     2701 gcgtcgaggt cggggatctc ctgggtgccg tcccactccg aataccgatg gacgaggacc
     2761 atcttgcccc cctgtcgggc gcgcgtcccg ccgctagcct cggtaacgcg actggccgat
     2821 cacgctgtcc ttgttcagcc gcttgttgag atgcaacccc tcgagcacca gctccaccgc
     2881 cgccgcctgc gccgccggac tcgcgtccga ggtcacctgc cgcaccgccg gagagagccc
     2941 gtcgacctgc ttcagcagct gctggtagtg cgagaccggc agtgcctcgc cggtctccgc
     3001 cgagattcct gacttgaacg ccgcgatcac cggctccagg tccgtaatgc tgcagcggcg
     3061 gttgaacacc gccaccaccg cgccctggat cagccggtcg acgatctggt cttcgcgccc
     3121 gtcctcgacg gtctcaaact cgaccttgcc ttcgagggcc ggcttgatga actgcaggtc
     3181 cgagatccgc ggcgccacct cgcgctcccc gagccgcacg gcacgccgga acgcgttcgc
     3241 caccatcgcc tcgtagtcag cgaccgaggc ccgcaccgac acacccgacc gctggttgat
     3301 gtccggtgac cgccgcgcga gccgcgtgat ttccgcgacc acttctttca tgtagtcggg
     3361 cacgacgacc tggtagccct cctgcgagag ctgcgccgcc tcctggtcga tgatgctgac
     3421 ctcgtcctcg acgtttcgag ggtagtgcgt gcggatctgc gacccgtacc ggtcctttag
     3481 cggcgtgatg atccgcccgc ggttcgtgta gtcctcgggg ttcgccgtcg ccacgatgaa
     3541 cacgtccagc ggcaaccgca ccttgtggcc gcggatctgg acgtcccgct cctccatggc
     3601 gttcagcaat cccacctgga tgcgctccgc caggtcgggc agctcgttga tcgcgaagat
     3661 gccccggttc gtccgcggga tcaggccgaa gtggatagtc agctggtcgg agaggtaccg
     3721 cccttccgcc accttgatcg ggtcgacctc gccgatgagg tccgcgatcg tgatgtccgg
     3781 cgtcgcgagc ttctcgccgt atcgctcgtc acgatggacc caggcgatgg gcgtgtcgtc
     3841 gcccatcgcc tcgatgagcg accggccctg ctcgctgatc ggcgcgaacg ggttctccgg
     3901 aatctcgacc cccgcgatca ccggcgtcca ctcatcaagc agcgaggtca ggctgcggat
     3961 gatgcgcgac ttcgcctggc cgcgttcccc cagcaggatg atgtcgtgcc ccgacaggat
     4021 cgcgttctcg agctgcggga tgaccgtatc ttcgtacccc gtgatcccgg ggaagagcgt
     4081 caccccctgc tcgaggtttg cgatcaggtt cttgcggatc tcctcgcgca ccggcaggac
     4141 ccggtacccg gatgcacgca gcgcgccaag cgtcgcgggt tgctcgttcg ccaaggctgg
     4201 cccccttcac ttccccggtc tctccggggc ggcgcgccgg cgggtgctgg tggttgagcg
     4261 aatgccgaac gctaccggtg gctccccacc ggtcgcctcg atcagtatac gtttccccgt
     4321 acccgctgaa cgggacgcca aaaggccccg ccagagcggg gcccttcgtc ggtgggagga
     4381 atggtcgagg agttaggacg ccgcttccga ctccacgcgc cggggcgcaa tcgagtccag
     4441 caggtccggc cggtccacga gcgtcttcac gaccttacca agccgcgagt cacgcagcac
     4501 gatgatgtcg gagacatcga gcacgtcgcc cagcgagatg tcgagcgccg ccgctaccga
     4561 gagcgccgtc gacagcggaa gctcgcgctt ccccgtctcg tagttcgaga gcgaggcctg
     4621 cgtgatgccc gccgtccgtg cgacttccgc ctgcgagcgc tcccgcttca tccgccactg
     4681 ccgcagccgc tgcccggttg cgcggcgtcg ctctcgctcc gcggcttcaa ggttcgtcgt
     4741 catgtagtcc tttcctttcc aggggcgcct cgtccctgca gagatatctg gtcgtgattc
     4801 taccggatgt gttcttgtgc gcacaagcac aagttcagag cgcggctcta tcacgtttgc
     4861 gtggatcgag cataggaccg ggatcgaggt atgggaagcg cgctacggta acacttggat
     4921 gacagccgag cgtccgacca cctgctgcca ggcccagccc tcgccgctgc tcatgaacca
     4981 gatgtcgcgg tgctgcccgt ggatggcgct ccccgtgtcc gcgcacacgt acgtccggcc
     5041 ggtcgggtct ccctcgatgc ggaagcgctg accgaggtag accacgtcgc acgctgccgc
     5101 acccgggtag accgccgttc cgtcgcgcat caggccacag aacccgccgc catccccgcg
     5161 ccgctgacca cccgtcgagt cttcgcagta atagaaggtg accggcaccg tcagctcgtc
     5221 gcccggccgc atgtcctgcg ccgtcggcgc cgtgacctgc gtccgagtcg ttgcctcgct
     5281 cactgtcggg gtcgacttca ccttgaaccg cgccgccgag accaccatgt cgccctggtc
     5341 cgccggccgc tccgccagca tcggcacttc gttggtcagc gcggtcgaga ctcgagcttc
     5401 tgacgccgcg ccttcctcag cgctcgcgcc ctcggaggct ccggccgtgg cttccggttc
     5461 cgctgtcgcg gtcgttgccg ccgactcggg cccgccgtct gcgctcgcgg ccttcacgga
     5521 cccaggcgac ttcacccacc tggtgagtgc cccgatcgag gtggactccc cgtgatcgcg
     5581 gctgctgctg gcgctggtct tcggatacgt cgtgccgtcg ttgccctgca gcacgacccc
     5641 ggtcacgacg gcagcgacgg cgatcgtcaa cgccagcccg gcaatgaaca ggctgtggcg
     5701 caacaaccgc cagggcgtca gaaggtggcc ccaccgcata ggcgcgcatt atggcacctg
     5761 atcgccgctg agcgcacctc ttatcaacac aactgctcaa atcgctgtgg ataagttatg
     5821 tgcgccttga ggccgctcgc gcgctcttat gttgcctcag tcgagcagcc ccgccgcgcg
     5881 caggtcaccc accgccgccc gaaacgcctc taccgtctgg tcgatctcct cgtccccatg
     5941 cgccgaggac accatccccc cggtgttcat caggtccacg ccgtggttga acagcgccca
     6001 cttcagcggc gctaccagcc gttccggcac cccctgtggc tcctcgctcg cgtcccactc
     6061 gacgtccgtc ggccgcggcg cctcgtaccc gaggttcaaa tgccacatcg aggaaaccgc
     6121 ccaggcggac cccggcacgc tcaactcccc gaacagcgag ttcagtccgc gcaccagccg
     6181 tgccgtcgcc gcgtccgc
//
LOCUS       DAORPG010000029         6162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_655_length_6162_cov_3.676601, whole genome shotgun sequence.
ACCESSION   DAORPG010000029 DAORPG010000000
VERSION     DAORPG010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_655_length_6162_cov_3.676601"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1432)
                     /gene="nhaA"
                     /locus_tag="PLX85_01260"
     CDS             complement(95..1432)
                     /gene="nhaA"
                     /locus_tag="PLX85_01260"
                     /inference="COORDINATES: protein motif:HMM:NF018648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HRC61835.1"
                     /translation="MDRSPRRTRPALNRLVEPLQEFVATEAAGGLVLLAAAIVALVWA
                     NSAWSSSYDDLLHHHIVVDLGFYRIDESVHFWVNDVLMVLFFFLVGMEIKRELVIGEI
                     SSVAQASVPLAAALGGMILPVAIFLAIAGRSEGGNGWGVPMATDIAFALGIAALAGPR
                     VPTGLKVLVLALAIFDDLGAVAVIAVAYGESIHFGPLALGLALLPAVYLMNRAGVREL
                     SLYVIAGLVAWAAIVKSGVHPTTVGVALGVLTPLDSRYPLGHLREEAEAVLRSLGDER
                     TAHDRQTGRHHHAEAILRLRRLAEDAVAPLIRLEHGLNYWVAFVVVPTFALANAGVDL
                     RGDTLSGAFSDPLTWGIALGLVLGKPLGIILGAWIAVRLGARLPNGATWRGVVAVGAI
                     AGIGFTVALFVAELAYADSRLLAFSKVAILSGSVVSGCVGYILLKTLPAEATS"
     gene            1486..1779
                     /locus_tag="PLX85_01265"
     CDS             1486..1779
                     /locus_tag="PLX85_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HRC61836.1"
                     /translation="MTTTPVAPVAAPTHRSLTETLPPYRVILHNDDVNDMLHVVRALL
                     SSIPELTPERAIEVMLRAHQFGEAEAIQCPLERAELYRDRLQSHGLTATIERA"
     gene            complement(1786..2301)
                     /locus_tag="PLX85_01270"
     CDS             complement(1786..2301)
                     /locus_tag="PLX85_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HRC61837.1"
                     /translation="MTGWRAYLQLSDAELLAQCEFDRFRASGPGGQKRNRTDSAVRLR
                     HRPTGLQSEAVESRSQHENRARALRRLRLTIAIALRAPKADATAGEQRVLRSLTADPP
                     SRLRPVDFSSVALLFDVLDEHAWRISDAADALELSTAAVGRFLQVDPRVWRAAAERRL
                     ALGLSPLRAGR"
     gene            2396..3451
                     /gene="argC"
                     /locus_tag="PLX85_01275"
     CDS             2396..3451
                     /gene="argC"
                     /locus_tag="PLX85_01275"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRC61838.1"
                     /translation="MYRAQIVGATGYGGLGILELLLQHPNFEVASLVARDDAGRRIDE
                     VYPHLMGRCSLTVEAPDSGVIGTDCDVVIFATPDRVSMGFAAQLADAGRRFIDYSGDF
                     RFGTTEQYAEYAAAHPSIADPAHGAADLLGRAAYGIPELFGDRVTAAPIVGNPGCFAA
                     GMIIGLAPLFADRMVEGNEVIVDALTGSSGAGKKPAPLQHFSHLNDNVVPYRVFAHQH
                     VTETSQTLGALSGSPATVHFVPHLLGVTRGILSTMHVVLARPASKQELLERYREFYDG
                     QPFVRVLPTPPTLKGTAGSNYCDISVEVSPDGRRAVVMSSIDNLLKGQSGTAMQNVNL
                     MFGLDPRAGLDRPPLYP"
     gene            complement(3463..4005)
                     /locus_tag="PLX85_01280"
     CDS             complement(3463..4005)
                     /locus_tag="PLX85_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61839.1"
                     /translation="MAKDDRSTVACLSVAVASTVLLGLGGWLWWVLSGLGDVRGSDSV
                     FVKSVIFGSVASLGLWGVWLLIVVALMQRFAGTIVQIEPLLRAAGLAAAPLAFGFLMV
                     VPVVSFGIGLFAIAGCVLLTQAAVERVTGVRGGEVIFANLAGFIVWAVVLSLLTTRSN
                     QFAPGPFLAESLWDLVASAS"
     gene            complement(4128..4874)
                     /gene="rsmG"
                     /locus_tag="PLX85_01285"
     CDS             complement(4128..4874)
                     /gene="rsmG"
                     /locus_tag="PLX85_01285"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRC61840.1"
                     /translation="MSTTPSSLEFLRSEAFRLGLQLDDVALDRFERYLTLLLEWRERA
                     GLTSISDPLTIQQRHFVESVALLVALRGGGLLHDEGGKIADLGTGAGFPGLPMRIVDP
                     TLQLTLIESSSRRCRFLETVVDALHLDGVAVIQARAEEAGHDPVLRAGFDLVVARALA
                     ALPILVEYALPLLREGGILAAPKGSRGASELEAAAGAIAALGGQAEGSLALPLSEDVP
                     PQRVYMVRRVGRLDERFPRRPGVPSRRPLR"
     gene            4864..5748
                     /gene="greA"
                     /locus_tag="PLX85_01290"
     CDS             4864..5748
                     /gene="greA"
                     /locus_tag="PLX85_01290"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRC61841.1"
                     /translation="MVDTDSRQSSRLLVTPRLPWHTTHLKSGGPPLMAEAITTTEAAA
                     HFLNSLEPEKAQEERIQVDHFVEKIGPEREVGSLTGADIDAYVAAEHERVGSSSHLEP
                     LRGFLAYCSRMAFTHQNLVPNLGLSGGARGDAAVEELGGKAYYVTIEGLAALERELEE
                     LKGRRPEIAEQLRAAMADKDFRENAPLDAARDAQAHLEARIRAIEDQLRHAVIIDPDQ
                     KGGRANVGSTVRVMNLKMNREQTFHLVSPNEVDPSNGKISVESPVGKAVINHAVGDEV
                     TVNAPSGELQLRVLEVIG"
     gene            5811..>6162
                     /locus_tag="PLX85_01295"
     CDS             5811..>6162
                     /locus_tag="PLX85_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRC61842.1"
                     /translation="MDRTKAVTRARFLTLIGGGALLGYGGARVWGRAIKGWRYNTVEK
                     PVPNFDREEYRLVVDGMVERPLTLTYSELRALPAIRQVSDFHCVEGWGVDDVQWDGVR
                     LQTIIDLVRPTSTAK"
BASE COUNT         1149 a         2024 c         2056 g          933 t
ORIGIN      
        1 cgcatcagta tcggttcatg gactcatttc cgcgtacggc gaacggcaag atccggcgtt
       61 cagagcttgt tcgggacttg ttggcggaag tccgttagct ggttgcctcg gctggcagcg
      121 tcttcagcag gatgtagccc acgcaaccac tcacgactga cccgctgagg atcgctacct
      181 tcgagaatgc gagcaggcgg gagtcggcat aggcaagctc ggccacgaac agtgcgacag
      241 tgaagccgat gccggcgatc gctccgacgg ccaccacacc tcgccacgtg gcaccgttgg
      301 gaagacgtgc ccccaagcgt acggcgatcc atgccccgag gatgattccc agcggctttc
      361 caagaaccaa cccgagcgcg atcccccacg tcaacgggtc gctgaatgca cccgaaagcg
      421 tgtcgcctcg aaggtctacc cccgcgtttg caagagcgaa ggtcggcacc acaacgaacg
      481 caacccagta gttcagtcca tgttcgagcc ggatgagcgg cgcgaccgcg tcctcggcga
      541 gccgccgcag ccgcaagatg gcctcagcgt ggtggtgcct gcctgtctgg cggtcatgcg
      601 ccgtgcgttc gtcgccaagt gagcgcagca cagcctcagc ctcctcccgc aggtggccga
      661 gtgggtacct ggaatccaga ggggtgagca cgccgagagc cacccccacg gtggtcggat
      721 ggacgccgga cttcacaatc gccgcccagg ccaccaggcc tgcgatgacg tagagcgaaa
      781 gctcacgcac gccggcccgg ttcatgagat agacagcggg aagcagcgcc agtccgagcg
      841 cgagcgggcc gaagtgaatc gactctccgt aggcgaccgc gatcaccgcc accgcgccca
      901 ggtcgtcgaa gatcgcgaga gcgagcacga gcaccttgag ccccgtcggc acccgggggc
      961 cagcgagagc cgcgatcccg agggcgaagg cgatatccgt ggccatcggc acgccccagc
     1021 cgttgcctcc ttcggaacgt ccggcgatgg cgaggaagat ggcgacgggc aggatcatac
     1081 cgcccaacgc ggccgccagc gggacgcttg cttgcgccac cgagctgatc tcaccgatga
     1141 ccagttctcg cttgatctcc atcccgacga ggaagaagaa gagaaccatc aggacatcgt
     1201 tcacccagaa gtgaacggac tcgtcgatgc ggtagaagcc gaggtcgacg acgatgtgat
     1261 ggtggaggag atcatcgtac gaactcgacc aggccgaatt ggcccagacg agcgcgacaa
     1321 tggccgcggc taacaggacg agaccgccgg ccgcctcggt cgcgacgaac tcctgcagtg
     1381 gctcgacgag ccggttcagc gcggggcgtg tccgtcgtgg cgatcgatcc atcggcctat
     1441 ctcggtctca ggaacgagcg ccgacagggg ttgaatgatg cgcccatgac gacgaccccc
     1501 gttgcacccg tcgcggcgcc cacgcatcgc tctctcaccg agacgctccc accgtatcgc
     1561 gtgatcctcc acaacgacga cgtcaacgac atgctgcatg tcgtgcgtgc gctgctgtcg
     1621 agcatccccg agctaacgcc agaacgggcc attgaggtca tgctgcgcgc gcaccagttc
     1681 ggcgaagccg aggccattca gtgtcccctc gaacgcgccg agttgtaccg cgaccgtctc
     1741 cagtctcacg gcctgaccgc cacgatcgag cgggcctgat cgaggtcatc gacccgctcg
     1801 gaggggactg agcccgagcg cgagacggcg ttccgctgcc gcgcgccaca ctcgtggatc
     1861 gacctggagg aaccggccta cggctgcggt gctcaattcc agcgcgtcgg cggcgtcgct
     1921 gatccgccat gcgtgctcgt cgaggacatc gaacagcagt gcgacgctgc taaagtcgac
     1981 cggacggagt ctggatggag gatcggcggt caacgagcgc aggacgcgct gctccccggc
     2041 ggtcgcgtcg gccttggggg cacggagcgc gatcgcgatc gtcagtcgca gacggcggag
     2101 cgctcgggct cggttctcgt gctgcgaacg cgactcgacg gcctcggact gcagtcccgt
     2161 cggccgatgc cgcaagcgga cggccgagtc ggtgcggttt ctcttctgtc cgccgggccc
     2221 gctcgcgcgg aagcggtcga actcgcactg agccagtagc tctgcgtcgc tcaactgcag
     2281 gtacgcccgc cagccggtca tgagaccgac ggtagcacgc tcgcgggctg actttcgcac
     2341 tctcgaaggc gcgcctattc ttggtgcttc actgcctggt ggaccgaggt gagcgatgta
     2401 tcgagcacag atcgtgggag cgacggggta cggcggactc ggcattctgg aactgctgct
     2461 gcagcatcca aacttcgagg tcgcatcgct ggtcgcccgt gacgatgccg gccgccgcat
     2521 cgatgaggtc tatccgcacc tgatgggacg ttgctcgctc acggttgaag cgccggatag
     2581 cggggtcatt ggaaccgact gcgatgtggt gatcttcgca acgcctgacc gcgtctcgat
     2641 ggggttcgcg gcgcagcttg ccgacgccgg gcgccgcttc atcgactatt caggtgactt
     2701 tcgcttcggc actaccgagc agtacgccga gtacgcggcc gcccaccctt cgatcgccga
     2761 tcccgcgcac ggcgcggcgg atttgctcgg gcgggccgcc tacggcatcc ccgaactctt
     2821 tggcgatcgg gtcacggcgg cgccaatcgt cgggaatccg ggctgcttcg ccgccgggat
     2881 gatcatcggc ctcgcgccgc tattcgcgga ccgcatggtt gagggcaatg aagtgatcgt
     2941 cgatgcgctg accggatcct cgggcgcagg caagaagccc gcgccgttgc agcacttctc
     3001 gcacctcaac gacaacgtgg tcccgtaccg cgtgttcgcg caccagcacg tgaccgaaac
     3061 gtcgcagacg ctcggcgcgc tgtcgggcag ccccgcgacg gtccatttcg tgccgcacct
     3121 gttgggtgtg acccgcggca tcctcagcac gatgcacgtg gtgctggccc gcccggccag
     3181 caaacaggag ctgctcgagc ggtaccgcga gttctacgac ggccagccgt tcgtgcgcgt
     3241 gctgccgacg ccgccgacgt tgaaggggac ggccggctcc aactactgcg atatctcggt
     3301 ggaggtttct ccggacggac ggcgggcggt cgtcatgtcg tcgatcgata acctgttgaa
     3361 ggggcagagc ggaactgcga tgcagaacgt caacctgatg ttcggcctcg acccgcgcgc
     3421 cggtctcgac cgtccgccgc tgtatcccta gcggggctcc cgttaactcg ccgaggcgac
     3481 gaggtcccag agcgattcgg cgaggaacgg tccgggtgcg aactgattgc tcctcgtcgt
     3541 cagcagcgag agcaccacgg cccagacgat gaagccggcg aggttcgcaa agatcacctc
     3601 gccgccccgc acgccggtca cccgttcgac cgccgcctga gttagcagca cgcagcccgc
     3661 gatcgcgaag agtccgatcc cgaacgagac gaccgggacg accatcagga agccgaacgc
     3721 gagtggggcg gcggcaagcc ccgccgcgcg caagagcggc tcgatctgca cgatcgtgcc
     3781 cgcaaaccgc tgcatcaggg cgacgacgat gaggagccac acgccccaga gaccaagcga
     3841 ggcgaccgaa ccaaagataa cgctcttgac gaagacggag tcgctgccgc gcacatcgcc
     3901 caggccgctg aggacccacc acaaccaccc cccgaggccg agcaatacgg tcgatgctac
     3961 ggcgaccgag aggcaggcga cggtcgaccg atcgtccttc gccacttcgt ccaggacatc
     4021 gagatccagg cgcgcgagtc ggcgcaggcg gtccagggtg agggtgagct ggccgggcag
     4081 ttgagggacc tttcgcagag cccggaaatt ctacaaccca ccgctcctta acgcaggggc
     4141 cgccgggagg ggacgcccgg gcggcggggg aagcgttcgt cgagccgccc gacacgcctc
     4201 accatataca cacgctgggg cgggacatct tcagacagtg gcagggccag ggaaccctcg
     4261 gcctgcccgc caagcgcagc gatggcccca gcggcagctt cgagctcaga cgcaccacgg
     4321 ctccctttgg gcgctgcgag gatgccgccc tcacgcagca gcgggagcgc gtactccacg
     4381 aggatcggca gcgcggcgag ggccctcgcg acgaccaggt cgaagccggc gcggaggacg
     4441 gggtcgtggc cagcctcctc ggcacgtgcc tggatgacgg ccactccatc gaggtgaagc
     4501 gcgtcaacga cggtttcgag gaagcggcag cggcgactac tcgactcgat cagggtcagt
     4561 tggagcgtgg gatcgacgat gcgcatcggg aggcctggga agccggcgcc ggtccccagg
     4621 tccgctatct tcccgccctc gtcgtgcagg aggcctccgc cgcgcagcgc gacgaggagg
     4681 gcaaccgact caacgaaatg gcgctgctga attgtgagcg gatcggagat cgacgtcagc
     4741 ccggcccgct cgcgccactc gagcagcagg gtcaagtagc gttcgaagcg gtcgagcgcc
     4801 acatcgtcga gctgcaggcc gagtcggaac gcctcggaac ggaggaactc gagggaggaa
     4861 ggcgtggttg acacggacag ccgccaatct tctagactgc ttgtaacacc acgcctgccc
     4921 tggcacacca cacacctcaa gagcggaggg ccgcccctga tggcggaagc gatcaccacg
     4981 accgaagcag ccgcgcactt cctgaatagc ctggaaccgg agaaggcgca ggaagagcga
     5041 atccaggtcg accacttcgt cgagaagatc ggcccggagc gcgaggtggg gtcactgacg
     5101 ggcgccgaca ttgacgcgta cgtggcggct gagcacgagc gggtaggcag ctcatcgcac
     5161 cttgagcccc tgcgtggctt cctcgcttac tgctcgcgga tggcattcac gcaccagaac
     5221 ctggtgccga acctcggcct gagcggcggc gctcgaggag acgcggcagt cgaggagctg
     5281 ggcggcaagg cgtactacgt gaccattgag ggccttgcgg cactcgagcg cgagctcgaa
     5341 gagctgaagg gacgccggcc cgaaatcgct gagcaactcc gcgccgcgat ggccgacaag
     5401 gactttcgtg agaacgcacc gctggatgca gcgcgagatg cacaggcgca cctcgaggcg
     5461 cgtatccgcg cgatcgaaga ccagctgcgg cacgcggtga tcatcgaccc cgaccagaag
     5521 ggcggtcgcg ccaacgtcgg ttccacggtg cgcgtgatga acctgaagat gaaccgtgag
     5581 cagacgttcc acctcgtgag tccaaacgaa gtggatccgt ccaacgggaa gatctccgtg
     5641 gagtcgccgg tcggtaaggc ggtgatcaac cacgccgtcg gcgatgaggt gaccgtcaat
     5701 gctccctccg gggaacttca gctgcgtgtc ctcgaggtca ttgggtaagc gcccagcctt
     5761 cagttcagga acgtaacgag tcgtctcata tcgcggtcta tgcttgatgc atggaccgaa
     5821 cgaaggcggt cacccgagcc cgattcctca ccctcatcgg cggcggggcg ctcctcggct
     5881 acgggggtgc tcgcgtgtgg ggtcgggcga ttaaggggtg gcggtacaac accgtcgaga
     5941 agccggtgcc gaacttcgac cgtgaagagt accggctggt cgttgacggc atggtcgagc
     6001 gcccgctgac ccttacctat agcgagttgc gcgcgttacc cgcgatccgg caggtgagcg
     6061 acttccactg tgtcgagggc tggggggtcg acgacgtgca gtgggacggc gtccggctgc
     6121 agaccatcat cgaccttgtc cggccgacct cgaccgccaa gt
//
LOCUS       DAORPG010000030         6131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_661_length_6131_cov_3.895984, whole genome shotgun sequence.
ACCESSION   DAORPG010000030 DAORPG010000000
VERSION     DAORPG010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_661_length_6131_cov_3.895984"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PLX85_01300"
     CDS             complement(<1..750)
                     /locus_tag="PLX85_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRC61843.1"
                     /translation="MNTAEILMIAGSMVPDRVALSDPKGSVTYSDLQSRVNKLANALQ
                     GLGVGKGANVGIMAVNSNQFVELYYATASVGATYVPLNFRAKTEELQYMLDASDVNVL
                     FVSERYFGILEQIKGSLPKIQKVYTLDFTADGHETYESLLASGEDSMVFADVDDDDAA
                     LVIYTSGTTALPKGVVLTHKTLTAYVVNTQNPVDPSLDQEVTLVAVPFFHVAGATTML
                     GSLWSGRKLAILPQFDPTGWLDMVEREGVTHA"
     gene            complement(810..1166)
                     /locus_tag="PLX85_01305"
     CDS             complement(810..1166)
                     /locus_tag="PLX85_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61844.1"
                     /translation="MWLWPSGDGQIDIGRIEDYRPGSVVYRSTDGFFVAAQPDGSVLA
                     LSDIDPHNPPGRRSCRVTFRPDLGVTGETGRFFDGCTGSLYDIGGRALSGDGLDLKRL
                     PVQRHEDGSIEVKPAR"
     gene            complement(1395..1844)
                     /locus_tag="PLX85_01310"
     CDS             complement(1395..1844)
                     /locus_tag="PLX85_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61845.1"
                     /translation="MVGLALVAVFGIGGVVWGGLFAGTHIEVQQLDAGPVSQFAIGQV
                     VAYPNVNVYLVGIDDGRIRAVDGIVKESGCAVRWDPADERTRAANAGHGVGSYTDPCS
                     GDVWTNAGNAFSGAKTPLRTFQVNYETNDEGVQHVWVEVIGNRDSRP"
     gene            complement(1959..2705)
                     /locus_tag="PLX85_01315"
     CDS             complement(1959..2705)
                     /locus_tag="PLX85_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRC61846.1"
                     /translation="MTASGSDDVAPFVIAEREGDVVWLTLNRPSRLNALHLEMRDELW
                     LQLCLLRDDPSVRVAVLRGAGDRAFSAGADITEFGTAPSYVDAREARTDRDLWGLMST
                     LPVPLVAAVHGYAYGAGLEMSLYCDIRIASEDARFAVPEVTLGYIPSAGGSQTLARHV
                     PPTFALRMATTGEPITAREAYDLGLVHEVVPPTDLDAAARRWADLLATRPPGAIRATK
                     RAIIEGLDLPLDQGLALERRLAAGLRAGPR"
     gene            complement(2712..2951)
                     /locus_tag="PLX85_01320"
     CDS             complement(2712..2951)
                     /locus_tag="PLX85_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61847.1"
                     /translation="MKVEFTAEEVQAMVNYVLDQLGAIEELDRKDRATLRRWRGDDLR
                     LGSPTMQLLTDRVNAELQRIHEAAHVSAITKPDWL"
     gene            complement(2948..3214)
                     /locus_tag="PLX85_01325"
     CDS             complement(2948..3214)
                     /locus_tag="PLX85_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61848.1"
                     /translation="MNVILDLDEVHAVLARVTGRVIDDAGLSEAGCEAIRRWRLDHDH
                     GTQALDDFVVGFNEALGNHIDDSTSRRLRLRGGIVVSLKDTVKR"
     gene            complement(3276..4031)
                     /locus_tag="PLX85_01330"
     CDS             complement(3276..4031)
                     /locus_tag="PLX85_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRC61849.1"
                     /translation="MPLTSLSIEDYVATLTLYPAADGVSRDFVDALADSCEEVAGARG
                     RAYAVVVRCVPEFPSRWSPEALAEPVLNGVRPLGAAFDALASLPQPTIAAVRGAAHSA
                     GLELALACDMRVAANSATFAAPETGLGMMPLGGATQRLPRAVGRSQALRLLLSGLEID
                     AVEARRIGLLVEVVSEGGEVDAAVRLARTIASRAPIATRFAKEAVWRGLEQPLQQGLR
                     TELDLTVILQTTADRAEGVRAFVEGRTPRFTGS"
     gene            4187..4657
                     /locus_tag="PLX85_01335"
     CDS             4187..4657
                     /locus_tag="PLX85_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005147259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRC61850.1"
                     /translation="MASWGTIATAEPKFAHAVQSVFDSHKHKVLATLRRDGSPRLSGI
                     EATFAAGELWLGMMPNSFKAHDLLRDPRMALHSATVDVELLQGDAKLSGRAIEATGPH
                     AWDVLNLEAEIPEESHLFRIDVSEVSLTRIGEPRDHLVIESWSEARGLRSVKRY"
     gene            4725..5654
                     /gene="ispH"
                     /locus_tag="PLX85_01340"
     CDS             4725..5654
                     /gene="ispH"
                     /locus_tag="PLX85_01340"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014359464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRC61851.1"
                     /translation="MPHTLLLAEPRGFCAGVVRAIDVLDAVLDREPAPVYAFHEIVHN
                     RHVVDGFRQRGAIFVDSLEEVPQGSRIVFSAHGVSPAVVEEAERRQLRVIDATCPLVT
                     KVHLETRKAARDGFEILLVGHRGHDEVVGTLGEAPERTSIVDSAEDVRALSERTKPVF
                     VVTQTTLSVDDTAGVIDEIRSRYPEAQVRNDICYATTNRQLAVRTIAERSDLVLVVGS
                     INSSNSVRLQQVAASTGTPAYRIDGIEEIDPAWYDGVEVVGLTAGASVPDELLDPIIE
                     DLKRRGVTRVEPVVVAEETVVFQPPSGLDPGRA"
     gene            complement(5665..>6131)
                     /locus_tag="PLX85_01345"
     CDS             complement(5665..>6131)
                     /locus_tag="PLX85_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61852.1"
                     /translation="IAGSTTTDFGAPGAIPLADAEPASPAEAERLVDLVRAAWTVLDE
                     VAASAPASLAKGPRGGGRDRDKMLQHVLSAEASYARYLGVRHREPAFDDLAAVADVRT
                     AVAGALSDRLTSAPAEGQRWPARYAARRVAWHVLDHAWEMEDRGGLDPRRSG"
BASE COUNT          981 a         1983 c         2224 g          943 t
ORIGIN      
        1 ggcgtgcgtg accccctcgc gctcgaccat gtcgagccag ccagtggggt cgaactgcgg
       61 caggatggcg agcttgcggc ccgaccagag cgagccaagc atcgtggtgg cgccggcgac
      121 gtggaagaag ggcaccgcga cgagcgtgac ttcttggtcg aggctcgggt cgaccgggtt
      181 ctgggtgttg acgacgtacg cggtcagcgt cttgtgggtg aggacgacgc ccttcggcag
      241 ggcggtcgtg ccggaggtgt agatgacgag ggcggcgtca tcgtcgtcta cgtccgcgaa
      301 caccatggag tcctcaccgg acgcgagcag cgactcgtag gtctcgtgcc cgtcggcggt
      361 gaagtcgagg gtgtagacct tctgaatctt gggaagactg cccttgatct gctcgaggat
      421 gccgaagtag cgctccgaaa cgaagagcac gttcacatcg gaggcgtcca gcatgtactg
      481 gagctcctcc gtcttggcgc ggaagttcag cgggacgtag gtcgcgccca ccgaggccgt
      541 ggcgtagtag agctcgacga actggttcga gttgacggcc atgatgccga cgttggcgcc
      601 cttgccgacg ccgaggccct ggagggcgtt cgccagcttg ttcactcgcg actgcaggtc
      661 gctgtaggtg acggagccct tggggtccga cagcgcgacg cggtcgggaa ccatgctgcc
      721 ggcgatcatc aggatttctg cggtgttcaa gtttcgtccc ccctactccc ggcagcgccg
      781 gcggtttgct ctgctgactc ttccgatcgt taccgtgcgg gcttgacctc gatgctgccg
      841 tcctcgtgtc gttggacggg cagccgcttt agatccagtc cgtcacctga caacgctcga
      901 cctccgatgt cgtacaggga gccggtgcaa ccatcgaaga agcgcccggt ctcgcccgtc
      961 acccccaagt ccggccggaa cgtcacccgg cagctacgcc gtcccggcgg attatgcgga
     1021 tcgatgtcgg agagcgcgag taccgagcca tccggctggg ctgcgacgaa gaaaccatcc
     1081 gtgctgcggt agacgacgct gcccgggcgg tagtcctcga tcctcccgat atcgatctgg
     1141 ccgtctcccg aaggccatag ccacatcacc acgacgacga gcgcggcccc gagcgcaacc
     1201 agaccgatgg caatcaggca gccggggcgt acgcccgtcg gccctgccgg ccggtcgtca
     1261 gtctcggtta cggcgtcacc ctcctcgatg gccgccgcat ccccgcgccg aagcgtcgct
     1321 catcatagac aacggttatt ctgggcgcca tacgtgcatc ttgactggat acatatagac
     1381 gcgagggggc cgcgtcaagg gcgagagtcc cggttcccga tcacttcaac ccaaacgtgt
     1441 tggacgccct cgtcgttcgt ctcgtaattg acctggaagg tgcgcagcgg cgtcttcgct
     1501 ccggagaagg cattgccggc gttggtccag acgtcgccgg agcagggatc ggtatacgaa
     1561 cccacgccat ggccggcgtt cgccgcccgg gtccgctcgt cggccggatc ccaacggacg
     1621 gcgcagcccg actccttcac gatgccgtcc accgcgcgga tgcgcccgtc gtcgatgccg
     1681 acgaggtaga cgttgacgtt cgggtaggcg acgacctgcc cgatggcgaa ctgactcact
     1741 ggcccggcgt cgagctgctg cacctcgata tgcgtgccgg caaacaggcc gccccagacg
     1801 acgccgccga tgccgaagac ggcgaccagc gcaagaccga ccaccgtgag gcggccgcgc
     1861 gcgtcccagc gccggaagcg ttgcccgatc cgagcgagca gcgggggcgc ggcgggacga
     1921 gaggccgaat cgcgagcgat ggtgtggcgc tcctcgggtt agcggggccc agcacgcagc
     1981 ccggccgcga ggcggcgctc gagcgcgagg ccctgatcga gtggcagatc gagtccttcg
     2041 atgatggcac gtttcgtcgc gcggatcgcc cccggcgggc gggtcgcgag cagatcagcc
     2101 cagcgccggg cggccgcatc gagatcggtg ggcggcacga cctcgtgcac caggccgagg
     2161 tcgtaggctt cccgggcggt gattggctcg ccggtcgtcg ccatgcggag agcgaacgtg
     2221 ggggggacgt ggcgggccag agtctggctg ccgcccgcag aggggatgta gccgagggtt
     2281 acctcgggga cggcgaagcg agcgtcctcc gacgcgatac ggatgtcgca gtagagggac
     2341 atttccaggc cggcgccgta cgcatagccg tgcaccgcgg cgacgagcgg tacagggagc
     2401 gtcgacatca gaccccagag gtcgcggtcc gtgcgtgcct cgcgcgcgtc gacgtacgac
     2461 ggggcagtgc cgaactcggt gatatctgca ccggccgaaa aggcacggtc gcccgcgcct
     2521 ctcaggaccg ccactcggac cgacgggtcg tctcgcagca ggcagagctg caaccagagc
     2581 tcatcccgca tctcgaggtg cagggcgttc agcctcgagg ggcggttgag cgtgagccag
     2641 acgacgtcgc cctcgcgctc ggcgatgacg aagggggcga cgtcgtcgga cccgctcgcg
     2701 gtcatgcctg actagagcca gtccggcttg gtgatggccg agacgtgagc cgcctcgtgg
     2761 atgcgttgca gttcggcgtt cactctgtcg gtaagcagct gcatagtcgg cgagccgagg
     2821 cggagatcgt cgccccgcca gcggcgcaga gtagcgcgat ccttgcggtc gagctcctcg
     2881 atggcgccga gctggtcgag gacgtagttg accatggcct ggacttcttc ggccgtgaac
     2941 tcgactttca tcgcttcacc gtgtccttga gcgagaccac gatgccaccg cgcaggcgga
     3001 gcctgcggct ggtgctgtcg tcgatgtggt tgcccagcgc ctcattgaag cccacgacga
     3061 agtcgtcgag ggcctgcgtc ccgtggtcgt gatcgagacg ccagcgccgg atggcctcgc
     3121 agcccgcctc ggagaggccg gcgtcgtcga tgacgcggcc cgtgacacgg gcgagtacgg
     3181 cgtgcacttc gtccagatcg aggatgacgt tcaagcgttc cccctttctc gtttcctatc
     3241 ggactttcag ctgaatcgcc cgcagccggc gggaatcagc ttcccgtgaa gcgtggcgta
     3301 cggccctcca cgaacgcgcg cacaccctcg gcacggtcgg ccgtcgtctg caggatcacg
     3361 gtgaggtcga gctcagtgcg caggccctgc tgcagcggtt gctcgagccc tcgccagacg
     3421 gcctctttcg cgaagcgagt cgcgatcggg gcgcgcgagg cgatcgtacg agcgagccgc
     3481 acggcggcgt cgacctctcc accttctgat acgacctcga cgagcaggcc gattcgtcga
     3541 gcctcgacgg cgtcgatttc gaggcccgag agcagcaggc gcagcgcctg ggagcggccg
     3601 acggcacgcg gcaggcgctg ggtggcgccg ccgagcggca tcatgccgag gccggtctct
     3661 ggagcggcga aggtggcgga gttggcggcg actcgcatgt cgcaggcaag cgccagctcg
     3721 aggccagcgg aatgcgccgc gccacggacc gcggcgatgg tgggctgcgg cagactcgcg
     3781 agcgcatcga aggccgctcc gagtggccgg acgccgttca gcacgggctc cgcaagcgcc
     3841 tcgggagacc aacgcgaagg aaactcgggg acacagcgga cgaccacggc gtaggcgcgt
     3901 ccgcgagcgc cggcgacctc ctcgcaggag tcggccagcg catcgacgaa gtcgcggctc
     3961 acaccgtccg cggcgggata gagcgtgagg gtggcgacgt agtcttcgat gctgagcgag
     4021 gtgagcggca cgtgcggcct cggtccccgg ggaaggctcg tcggttgagc ggtgggcatc
     4081 gtacgggcct ccagcgaggc gggtcaacgc ggactggagg cgcatcctgc ggtgcgccgc
     4141 ccgacctcgt acgctcgtca gcaggagttg gaaggagcgc gtgacgatgg cgagctgggg
     4201 aacgatcgcg acggccgagc cgaagttcgc gcacgcggtg cagagcgtgt tcgacagcca
     4261 caagcacaag gtgctggcga cgctcaggag ggatgggtcg cctcgactga gcggcatcga
     4321 ggcgacgttc gccgccggtg aactctggct gggaatgatg ccgaattcgt tcaaggcgca
     4381 cgacctcctg cgcgaccctc ggatggcgct gcacagcgcg acggtcgacg tggagttgct
     4441 gcagggcgac gcgaagctgt ccgggcgcgc catcgaggcg acgggcccgc acgcgtggga
     4501 cgtgctgaac ctcgaggcgg agatcccaga agaatcgcac ctgtttcgca tcgatgtgtc
     4561 cgaggtatca ctgacgcgga tcggcgagcc gcgcgaccac ctcgtcatcg agtcgtggag
     4621 cgaggcgcgc gggctgcgca gcgtgaagcg gtattgaggt cgattcgcac gcccgaaggt
     4681 tcttcaatcg ggccgtctcg atacaatgga agcccggagg tggtatgccc cacaccctgc
     4741 tgctcgccga gccgcgcggg ttttgcgcgg gcgtcgtgcg cgcgatcgat gtgctcgatg
     4801 cggtgctgga ccgcgaaccc gcgcccgtct acgcgttcca cgagatcgtg cacaaccggc
     4861 acgtggtcga tgggttccgc cagcgcggag caatcttcgt cgacagcctg gaggaggtgc
     4921 cgcaagggtc gcgcatcgtg ttcagcgccc acggcgtgtc gccggccgtc gtcgaggagg
     4981 ctgaacgtcg ccaacttcgc gtgatcgacg cgacctgccc gctggtgacg aaggtgcacc
     5041 tggagacgcg gaaagcggcg cgggacggct ttgagatcct gctcgtgggg caccgcggtc
     5101 acgacgaggt cgttgggacg ctcggcgagg cgccggaacg cacctcgatc gtggactcgg
     5161 ccgaggacgt gcgcgcgctg agtgagcgaa cgaagccggt gttcgtggta acgcagacga
     5221 ccctctcggt cgacgacacg gcgggggtca tcgacgagat ccgctcgcgg taccccgagg
     5281 cccaggtgcg caacgacatc tgctacgcca ccaccaaccg gcagctggcg gtgcggacga
     5341 tcgcggagcg gtccgacctc gtgctcgtgg tggggtcgat caattcgagc aactcggtgc
     5401 ggctccagca ggtggccgcg agcacgggga cgccggcgta tcgcatcgac gggatcgagg
     5461 agatcgaccc ggcctggtac gacggggtcg aggtcgtcgg tctgacagcg ggggcgtcgg
     5521 tgccggacga gctgctcgac ccgatcatcg aggacctcaa gcggcggggc gtgacgcggg
     5581 tggagccggt cgtggtggcg gaggagaccg tggtcttcca gccgccgagc gggctcgacc
     5641 ccgggcgggc gtagcctcgc ggcgctaacc ggagcggcgg ggatcgaggc cgccgcggtc
     5701 ttccatctcc caggcgtgat ccaggacatg ccaggcgacg cggcgcgcgg cgtagcgggc
     5761 cggccagcgt tgtccctcgg cgggcgccga ggtcaggcgg tcgctgagcg ccccggcgac
     5821 cgccgtgcgt acgtcggcga cggccgcgag gtcgtcgaag gctggctcac gatggcgcac
     5881 gccgaggtag cgcgcgtagc tcgcctcagc gctcaagacg tgctgcagca tcttgtcgcg
     5941 gtcgcggccg ccgcctcgcg gtcccttcgc gagtgacgca ggcgcgctgg ccgcaacctc
     6001 atcgaggacc gtccaggctg ctcgcacgag gtccaccagg cgctccgcct cggcgggcga
     6061 cgccggttcg gcatccgcga gcgggatagc gcccggtgcg ccgaaatcgg tcgtggtcga
     6121 acccgcgata c
//
LOCUS       DAORPG010000031         6029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_694_length_6029_cov_3.629394, whole genome shotgun sequence.
ACCESSION   DAORPG010000031 DAORPG010000000
VERSION     DAORPG010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_694_length_6029_cov_3.629394"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLX85_01350"
     CDS             <1..406
                     /locus_tag="PLX85_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61853.1"
                     /translation="TATPPAPPKADAATPAAVQGSGSGSTQVAPALPADSITSTPVAE
                     ERVAAPSIAPSTGSLGAGIGTDSPSGRVIVPAPAANDDWGQAAVRVSVTSREGIDSVR
                     ASEFGLGALALGLGGLTGWFWYLRRREAAANV"
     gene            419..1159
                     /locus_tag="PLX85_01355"
     CDS             419..1159
                     /locus_tag="PLX85_01355"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRC61854.1"
                     /translation="MRRSGAWIAIAMLGLGALALAACSSETRVAVDQQQQTGIAVSGT
                     GRVTVVPDIALVNLGVEVTRSSVADARSAAATAMDAVRASLKRSGVDDKDVATSYFSI
                     QPQYGSSEPLDKSGTPRIAGYTVVNQLAVKVRRIDDLSKVVDSAVAAGGDATRVQNIA
                     FTVDQPAQFEADARQKAVDDAHARAEQLAKLAGVNLGHARAVSESTAGVAATDLKLAV
                     PATGAGSTPFSPGQTEVSLTVNVVYDIE"
     gene            1253..1834
                     /gene="dcd"
                     /locus_tag="PLX85_01360"
     CDS             1253..1834
                     /gene="dcd"
                     /locus_tag="PLX85_01360"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HRC61855.1"
                     /translation="MILSDHSIAEALASGRLVIDPIGEDALQPASIDIRLDRQFRVFR
                     QHRRAYIDVRESVDDLTDVEEIEDDEPFVLHPGEFVLGSTLERVRLANDIVARVEGKS
                     SLGRLGLLVHATAGYVDPGWDGHLTMELSNVSNLPVRLYYGMKIGQLSFADLTTAADR
                     PYGDPRLHSKYQGQTGPTASRGYLDYESHTRPR"
     gene            1848..2957
                     /gene="ribD"
                     /locus_tag="PLX85_01365"
     CDS             1848..2957
                     /gene="ribD"
                     /locus_tag="PLX85_01365"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRC61856.1"
                     /translation="MPPSEFMRLALEEAALALGHASPNPAVGAVLVRDGEIVGRGHTQ
                     PPGAAHAEVMALRDAGERARGATAYVTLEPCGHQGRTPPCTRALVDAMVGRVVYAIED
                     PDPLVRGVGHRQLVEAGIAVEVGDGAEESGRLLEGYLKHRRTGMPFVIAKFAASLDGH
                     IAAASGDSRWVSGPETAAWMHRQRATLDAIVVGSRTVLVDDPQLTARPESATAATHQP
                     LRVVVDSSGRIPASARVLQPPPATLVATTEASAEEWRAAVRRAGAEVLTLPAHDGHVD
                     LDALLGVLGGRGVLTALFEGGGTLLGSLFDARLVDRVQAVIAPLIIGAAEAPSAVSGR
                     GAYRMADAPRLHAMTVERFGDDTMISGLPVWPDTD"
     gene            2966..3562
                     /locus_tag="PLX85_01370"
     CDS             2966..3562
                     /locus_tag="PLX85_01370"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRC61857.1"
                     /translation="MFTGIVEEVGTVQSAEGGVLVIGCRDIVEGTRLGDSIAVNGVDL
                     TVREMTAESMTFDVMPETFRRSNLRDAHSGDLVNLERSVRPNDRLSGHIVRGVVEATC
                     TLEGFTPEGEALIARFRPPAEYLRYIVMKGPVCVDGVSLTVMGRDETTFSVSLVQYTQ
                     EHTNLTRRQPGASINLETDIIARYVEQLLAERAAAPSS"
     gene            3586..4833
                     /locus_tag="PLX85_01375"
     CDS             3586..4833
                     /locus_tag="PLX85_01375"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HRC61858.1"
                     /translation="MDSPPRPDAELEPGLVPIEEAIEEFRQGRPVIIIDDKDRENEGD
                     VCIPAEFCTAEMVNFMAMHARGLICVAMTGDRLDDLGLPLMTSRNSSPLGTAFTVSVE
                     AREGVTTGISAADRARTIEVLIHPDSTSDDLTRPGHTFPLRARDGGVLVRAGQTEASI
                     DLAKLAGRYPAAVICEVMAADGTMARLPDLIEFAAEHNLKIVSVNQIIAHRLRNERLV
                     ERVADSLMPTRYGEFRAIAYRTLIDTREHVAFVMGDVTPDEPVLARVHDQCVTGDVFG
                     SLRCDCGEQLDKALRLVAEAGRGVVVYMDQEGRGIGLHNKIRAYHLQDGGLDTVEANE
                     ALGFPADKREYGIGMQILLDLGIRKMRLMTNNPIKRAGLEGFGLEVVERVPIEVTPNP
                     HNVRYLRTKREKMGHLLDADGLG"
     gene            4833..5309
                     /gene="ribH"
                     /locus_tag="PLX85_01380"
     CDS             4833..5309
                     /gene="ribH"
                     /locus_tag="PLX85_01380"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRC61859.1"
                     /translation="MRVIEGPLDGSALRVAVVVSRFNEMITEKLLKGATDTALEHGVP
                     EDCIDVVWVPGAYELPMAARRLAATGRYHAIACTGAVIRGQTPHFDFVAGEAARGIMD
                     VTRDFPIPVTFGVITSDNLEQAQARSGGAVGNKGREAMLAAIEMATLFPLIDRAHS"
     gene            5324..5680
                     /locus_tag="PLX85_01385"
     CDS             5324..5680
                     /locus_tag="PLX85_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61860.1"
                     /translation="MSPTSGTPSGGEPIDRTAGEAIRLAHRALDRFPEVVRRHKFVAG
                     GAVISSSLIALAGVAITRRIRAGQTPEEAVANVTAEEVQGLRVIEPEPPAESAEAAEQ
                     PDEPESDGAEAKRQLA"
     gene            5702..>6029
                     /locus_tag="PLX85_01390"
     CDS             5702..>6029
                     /locus_tag="PLX85_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRC61861.1"
                     /translation="MTIPSPIPRRIAHFDLDTFFVAVERLKDPTLVGQPVLVGGRGPR
                     SVVATASYEARVFGCHSAQPMTQALRLCPQAIVVPPDFASYRTASEGFHAVLREASPL
                     VESVGLD"
BASE COUNT          937 a         2170 c         2052 g          870 t
ORIGIN      
        1 gacggcgacg ccgccagcgc ccccgaaggc cgacgcggcc acgcccgcgg ccgtccaggg
       61 gagtggctcc ggctcaacgc aggtcgcacc ggcgctgcct gccgattcca tcacgtccac
      121 accggtcgcc gaggagcgcg tcgcggcgcc ctcgatcgcg ccgagcactg gcagcctcgg
      181 cgcgggaatt gggacagaca gcccgagcgg ccgagtcatc gttcccgcgc cagcggccaa
      241 cgacgactgg ggccaggctg cggtacgcgt ctccgtcacg agccgcgaag gcatcgattc
      301 ggtgcgcgcg agcgagttcg gcctcggggc gctggcgctt ggcctgggtg gcctcacggg
      361 ttggttctgg tatctgcgcc gtcgcgaggc ggcggcgaac gtctgagtgg agggcgagat
      421 gcgacgaagt ggcgcctgga tagcgatcgc gatgcttggc ctcggcgccc tcgcgctggc
      481 ggcgtgttcc agtgaaaccc gggtcgcggt cgaccagcag caacagaccg gcatcgccgt
      541 cagcggcacg ggccgtgtga cggtcgtgcc ggacattgcg ctcgtgaacc ttggcgtcga
      601 ggtgacgcgg tcgagcgtcg cggacgcccg gagtgccgcc gccaccgcca tggacgcggt
      661 gcgcgcctcc ctgaagcgga gcggcgtcga tgacaaggac gtcgcgacct cctacttcag
      721 catccagccg cagtacgggt cgtccgagcc gctcgacaag agcgggacgc cgcgcattgc
      781 gggctatacc gtcgtgaacc agctcgccgt gaaggtccgg cgcatcgacg acctgtcgaa
      841 ggtggtcgac tcggcggttg cggccggtgg cgacgcgact cgcgtccaga acatcgcctt
      901 tacggtggac cagcccgcgc agttcgaggc cgacgcccgc cagaaagcgg tggacgacgc
      961 gcacgcacgc gccgagcaac tcgcgaaact cgccggcgtg aacctcggcc atgcccgcgc
     1021 cgtctcagaa agcacggcgg gcgtggcggc gaccgatctg aagctcgcgg tgccggccac
     1081 cggcgccggg tccaccccgt tcagccccgg ccagaccgag gtgagcctca cggtgaacgt
     1141 cgtctacgac atcgagtagc cggaacggcg gaggttggcc gccacgggag cgacgcgata
     1201 ggatgaccgc agaggcgctg cgcgcctcgc tccccgtcga ggtggacctg agttgatcct
     1261 gagcgaccat tccatcgccg aagcgctcgc ttccgggcgg ctggtgatcg acccgatcgg
     1321 cgaggacgcc ctgcagcccg cctccatcga tatccggctg gaccgccagt tccgcgtctt
     1381 ccgccagcat cgccgcgcct acatcgatgt gcgcgagtcc gttgacgacc tgaccgacgt
     1441 cgaggagatc gaggacgacg agccgttcgt cctgcacccg ggtgaattcg tcctcggctc
     1501 cacgctcgag cgggtccgcc tcgccaacga catcgtcgcg cgcgtggagg ggaagtcgtc
     1561 gctcggccgc ctcgggctgc tcgtccatgc gaccgctggt tatgtcgacc cagggtggga
     1621 cggacatctg acgatggagc tctcgaacgt ctcgaacctg ccggtgcggc tctattacgg
     1681 catgaagatc ggccagctct cgttcgctga cctgaccacg gcggccgacc gcccgtacgg
     1741 ggatccgcgg ctccactcga agtaccaggg ccagacgggc cccaccgcga gccgcggcta
     1801 cctcgactac gaatcccaca cgcgtccgcg ctgagagccc tgcacccatg cccccatccg
     1861 agttcatgcg cctggctctc gaggaggcgg cgctcgccct cggacacgcc tcgcccaacc
     1921 cggccgtcgg cgcggtcctc gtgcgagatg gcgagatcgt cggacggggt cacacgcagc
     1981 cgccgggagc ggcccacgcg gaggtcatgg cgctgcgcga cgccggcgaa cgtgcccgtg
     2041 gcgcgaccgc gtacgtgacg ctcgagccgt gcggacacca gggacggacg ccgccctgca
     2101 cccgagccct cgtcgacgcc atggtcggcc gcgtcgtgta tgccatcgag gatccggacc
     2161 cactcgtgcg cggagtcggc caccggcagt tggtcgaggc cggcatcgcc gtcgaggtgg
     2221 gggacggcgc cgaggagtcg ggtcgcctcc tcgaggggta cctcaaacac cggcgcacgg
     2281 gtatgccgtt cgtgatcgcg aaattcgccg cctcgctcga cgggcacatc gcggcagcct
     2341 ctggcgactc aaggtgggtt tcgggcccgg agacggccgc ctggatgcac cggcagcgcg
     2401 ccacgctcga cgcgatcgtc gtgggttcac ggacggtgct cgtcgacgat cctcagctca
     2461 ccgcgcgccc cgaaagcgcc acggcggcca cccatcaacc cctgcgcgtg gtcgtcgact
     2521 ccagcggccg catccccgcc tcggcgcgcg tcctgcagcc gccgcccgcg acgctagtcg
     2581 caacgaccga ggcctccgcg gaggaatggc gggccgcggt tcgtcgagcc ggcgcggagg
     2641 tgctcacgct cccggcgcac gacggacacg tcgacctcga tgcgctcctc ggcgtgctcg
     2701 gcgggcgtgg cgtgctgacg gccctcttcg agggcggcgg cacgctgctc ggctcgctct
     2761 ttgatgctcg tctggtggac cgcgtccagg cggtgattgc gcccctcatc atcggggctg
     2821 ccgaggcgcc ctcagcggtc tccggccgtg gggcgtatcg tatggcggac gcgccacgcc
     2881 tccatgcaat gacggtggag cgcttcggcg acgacacgat gatcagtggc ctgcccgtct
     2941 ggcccgacac ggactaggag tcctcgtgtt cacaggcatc gtcgaggagg ttggcacggt
     3001 gcagagcgcc gagggtggcg tgctggtcat cgggtgccgc gacatcgtcg aagggaccag
     3061 gctcggcgac tcgatcgccg tcaacggcgt cgacctcaca gtccgcgaga tgacagccga
     3121 gtcgatgacg ttcgatgtca tgcccgagac gttccgtcgc tccaacctgc gcgatgccca
     3181 ttcgggcgac ctggtcaacc tcgagcggtc cgtacgcccc aacgaccgcc tgtccggcca
     3241 catcgtccgg ggcgtcgtcg aagcgacctg cacactcgaa gggttcacgc cggagggcga
     3301 ggcgctcatc gcgcgcttcc gtccaccggc cgagtacctt cgctacatcg tgatgaaggg
     3361 gcccgtgtgc gtggacggcg tgtcgctcac cgtcatgggc cgtgacgaga cgacgttctc
     3421 cgtctcgctc gtccagtaca cccaggaaca tacgaacctc acgaggcggc agccgggtgc
     3481 tagcatcaac ctcgagacgg acatcattgc ccgctacgtc gagcaactgc tcgccgagcg
     3541 cgcggccgca ccatcctcgt aacgtctcag ggcaggagag accagatgga cagcccacca
     3601 cgtccagacg ccgagttgga gcccggcctc gtccccattg aggaggcgat cgaggagttc
     3661 cggcagggcc gtcccgtcat catcattgac gacaaggacc gcgagaacga aggcgacgtc
     3721 tgcatccccg cggagttctg caccgccgag atggtcaact tcatggcgat gcatgctcgc
     3781 ggactgatct gcgtggcgat gaccggcgac cgcctcgatg acctgggcct gccgctcatg
     3841 accagccgca acagctcgcc actcgggacc gccttcaccg tcagcgtcga ggcccgcgag
     3901 ggcgtcacga ccggtatttc agcagccgac cgggcgcgca ccatcgaggt gctgatccac
     3961 cccgactcga catccgacga cctcactcgg ccgggccaca ccttcccgtt gcgcgcccgc
     4021 gacggcggcg tgctggtccg ggccggccag accgaggcct cgatcgacct cgcgaagctc
     4081 gccgggcgct accccgccgc cgtcatctgt gaggtgatgg cggccgacgg cacgatggcg
     4141 cggcttcccg acctcatcga gttcgcagcc gaacacaacc tgaagatcgt cagcgtcaac
     4201 cagatcatcg cgcaccgcct gcgcaacgaa cgcctggtcg agcgggtcgc cgacagcctc
     4261 atgccgacgc gctacggcga gttccgtgcc atcgcgtacc ggacgctcat cgacacccgc
     4321 gagcacgtcg ccttcgtgat gggagacgtg acgccggacg agcccgtgct cgcccgcgtg
     4381 cacgaccagt gcgtgacggg agacgtattc ggctccctgc gctgcgactg cggagagcag
     4441 ctcgacaaag cgcttcgcct cgttgccgag gccgggcgcg gcgtcgtcgt ctacatggac
     4501 caggaaggcc gcgggatcgg cctgcacaac aagatccgcg cctaccacct gcaggacggc
     4561 ggcctggaca cggtcgaggc gaacgaggcg ctcggcttcc ccgccgacaa gcgcgaatac
     4621 ggcatcggca tgcagatcct gctcgacctc ggcatccgca agatgcgcct gatgacgaac
     4681 aacccgatca agcgcgccgg tctcgagggc tttggcctcg aagtcgtcga gcgcgtgccg
     4741 atcgaggtca cgccgaaccc gcacaacgtg cgttacctgc gcaccaagcg cgagaagatg
     4801 ggccacctgc tcgacgcgga cgggctcggc tagtgcgcgt catcgagggc ccgctcgacg
     4861 ggagcgcgtt gcgcgtcgcc gtggtcgtgt cgcgcttcaa cgagatgatc acggagaagt
     4921 tgctcaaggg cgccacggac accgccctcg agcacggcgt cccggaagac tgcatcgatg
     4981 tcgtctgggt gccgggcgcc tacgagctgc cgatggccgc gcgccgcctc gccgcgaccg
     5041 ggcgctacca cgccatcgcc tgcaccggcg cggtgatccg ggggcagacc ccacacttcg
     5101 acttcgtggc cggcgaggcc gcccgcggca tcatggacgt gacgcgcgac tttccgatcc
     5161 cggtgacgtt cggcgtcatc acctccgaca acctggagca ggcgcaggcc cgctcgggcg
     5221 gcgccgtcgg caacaagggc cgcgaggcca tgcttgccgc gatcgagatg gcgacgctct
     5281 tcccgctcat cgaccgcgcg cactcgtagc agaggaggac ggcatgagcc cgaccagtgg
     5341 gacccccagc ggaggcgagc ccatcgaccg cacggccggc gaggcgatcc gcctggcgca
     5401 tcgcgcgctc gaccggttcc cggaggtcgt ccgccgccac aagttcgtcg cgggcggcgc
     5461 cgtcatctcc tcgtcgctga tcgccctggc cggcgtggcc atcacccgtc gcatccgcgc
     5521 gggccagacg cccgaggagg ccgtcgcgaa cgtgacggcc gaggaagtgc aggggctgcg
     5581 cgtgatcgag cccgaaccac ccgccgagag cgcggaggct gccgagcagc cggacgagcc
     5641 cgagtcggat ggagccgagg cgaagcgcca gctcgcctga cgtccgcccg atgaggtcct
     5701 cgtgaccatc ccgtccccga tcccccgccg tatcgcgcac ttcgacctcg acacgttctt
     5761 cgtggcggtc gagcgcctga aggacccgac gctggtcggc cagcccgtgc tcgtcggcgg
     5821 gcgagggccg cgctccgtcg tcgcgaccgc ctcctacgag gcacgcgtct tcggttgcca
     5881 ctccgctcag ccgatgaccc aggccctccg cctctgcccg caggcgatcg tcgtgccgcc
     5941 ggacttcgcg agttatcgga cggcgtccga agggttccac gccgtcctgc gcgaagcctc
     6001 gccgctcgtg gagtcggtcg gcctcgacg
//
LOCUS       DAORPG010000032         5992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_702_length_5992_cov_3.930773, whole genome shotgun sequence.
ACCESSION   DAORPG010000032 DAORPG010000000
VERSION     DAORPG010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_702_length_5992_cov_3.930773"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..763
                     /locus_tag="PLX85_01395"
     CDS             104..763
                     /locus_tag="PLX85_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61862.1"
                     /translation="MTRLALASLVAAVAVLALACSSNDKASTPTATPTAVATTTATTA
                     TAGTTAAASPPATRIPSEFTGLPAADTVIAAVLTGDLDRLVALAQLRQIACGPQTGVG
                     SPPPCPAGQTAGTPVPVFPVGTCEGEWRSETSLRASLQPLLNGPELYAVYGMPSQWQP
                     LMGDAQYVAVFSRNAPGQSRLGAGVVIAGGRIAGVWYGCGSFVDQIVPAGTSTLLGPR
                     R"
     gene            917..1192
                     /locus_tag="PLX85_01400"
     CDS             917..1192
                     /locus_tag="PLX85_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HRC61863.1"
                     /translation="MTYVITQPCIDTLDQSCVDVCPVDCIHFDEGVDRKLYIDPDECI
                     DCGACEPACPVSAIFAEDDVPADQAIFKEIDALWYQDKDAARAKIPA"
     gene            1301..2245
                     /locus_tag="PLX85_01405"
     CDS             1301..2245
                     /locus_tag="PLX85_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC61864.1"
                     /translation="MTIFETRALTKRYGSVAALDDFSIEVQPGVIGLVGANGAGKSTL
                     VKLLLGLIEPTAGAASVLGYDIRREGSHLRQYVGYMPEHDCLPLDMSAVEFVAHMCQM
                     SGLPRAAARERTAETLRHVGLFEERYRAMSGYSTGMRQRAKLAQALVHDPSIVFLDEP
                     TNGLDPGGRDEMLDLIRRTGHGLGISIVMATHLLSEIERVCDQLIVIDAGRLQHYGPL
                     AEFTEATQFLTVEVTADPEPVAAALRRRGLTCAVEGHHISVRVDDGSAPYDLIRDTVV
                     DLRVGLVRMSRRRHTLEDLFSVRQAQASEPVTAREAAS"
     gene            2245..3141
                     /locus_tag="PLX85_01410"
     CDS             2245..3141
                     /locus_tag="PLX85_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61865.1"
                     /translation="MVSTPGRAAPSNIYDLGYRHYEGLRLGRRHAMFVLYLESLRGAF
                     GLGRGAAAKVAPAALIFIAAIPALIQLLVSALFPGEAEVFAHHDYYRVISFVLGLYCA
                     AVAPDIAGRDQRNRSLTLYFSRAIERTDYAIAKLAAMASAMLLITLGPQLLLLVANGL
                     VAEDFSTHLRDNWHLLFPIAGSALVGSALIASMGIAIASQTSRRAFATVGIIVAFIVP
                     TAVARILVVGIDTSATHYGVFVSPLDVLGGLSSWMFRQPTPRGGAVAVAGFEGWTYLV
                     VALVVTAVASLLVLRRYWSVRA"
     gene            3138..4079
                     /locus_tag="PLX85_01415"
     CDS             3138..4079
                     /locus_tag="PLX85_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC61866.1"
                     /translation="MTAATSPVDAAPAIEVSETSRWYGNVVAVNDISFTVGPGITGLL
                     GPNGAGKSTLLHMLAGFLRPSSGTVRILGAPAWQNPGLYQQVGIVQERESVYDGLTGY
                     EFVRLSARLHHLPDVEAATRLAIDRVQMETAQGRKTGGYSKGMKQRIKIAAAIVHDPP
                     VLLLDEPFNGTDPAQRLQMMDLLRRMAAEGRTILFSSHILEEVERLAERVLVIVSGRL
                     AAAGDFRAIRRLMTDRPHTFAIVSSDNRALASALLADDSVTGAQIDGERITVRADDFE
                     RFTVHAPRIARSAGVTITEVDTLDESLESVFEYLVQR"
     gene            4076..4822
                     /locus_tag="PLX85_01420"
     CDS             4076..4822
                     /locus_tag="PLX85_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRC61867.1"
                     /translation="MIGLIFTLTLGQLLRQRRVLLLGLLAAIPVAITLLFRFTSSRAD
                     TGLPEFVASMLDALVVSIGLPLTALVLGTAALGQEIEDGTAFYLLSKPIARWQIVVAK
                     LMAAWVATSIFVTVSTLGSAAPLIGERGEDMLVPAFVIAGIVGALGYTTLFLALSIRF
                     NHALVIGLSYVFLWEGLASSLFKIDGVRFLSLRAYTLGIAGAISEAPSTVFSARLDGP
                     SASVLATILIVGAFIYAVRRLERYELSERV"
     gene            complement(4838..5644)
                     /locus_tag="PLX85_01425"
     CDS             complement(4838..5644)
                     /locus_tag="PLX85_01425"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HRC61868.1"
                     /translation="MTEARRPFRLLHTSDVHLGTFDFDRSGRWDERREQSEDRFRRVV
                     DVGIAERVDVFVIAGDFFDNARVSEETLRFAAEQIARIEVPTVIVPGNHDHVGKNSVY
                     DRIDLTSIAPNLTITRSVEGETVAFEGLDLELWARSHLEHDPDFSPFIDAPGRGDAPW
                     HIAVGHGHYWHPRSGDHPSFHIREEHFTSLDYDYVALGHWEQQTRIAAGDLLAAYSGA
                     PDGHASILGESGRVLVVDFQREGTVRLTSHPLDEGARILSHDEIPFLQGS"
     gene            complement(5652..>5992)
                     /locus_tag="PLX85_01430"
     CDS             complement(5652..>5992)
                     /locus_tag="PLX85_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRC61869.1"
                     /translation="IVLHLSPLVAPVTVAILPLSRNEQLTPTAREVYDLLRPHFRTQY
                     DDAQSIGRRYRRQDEIGTPLCVTVDFETIEDRAVTIRERDSMSQVRVPIDGLRAAIEE
                     RIDALRRAEG"
BASE COUNT          888 a         2218 c         1886 g         1000 t
ORIGIN      
        1 tgatggaacg gctcgaccgg gccatggccg acgcagcggc aggcggaccg ccgctggtgg
       61 aagccatggc aacgctgacg cccgacggga ggaacggccg ccgatgaccc gactcgcact
      121 cgcctcgctc gtcgccgccg ttgccgtgct cgcgctcgcc tgctcgagca acgacaaggc
      181 ctcgacgcca accgccaccc caacggcggt cgctacgacg accgccacaa cggccacggc
      241 gggcacaaca gccgccgcct cgccgccggc cacgcgcatc ccctcggaat tcaccggcct
      301 gcccgccgcg gacaccgtca tcgccgccgt tctcaccggc gaccttgacc gcctcgtcgc
      361 actcgcgcag ctgcgccaga tcgcctgcgg cccgcaaacg ggcgtgggct cgccgccgcc
      421 gtgccccgca ggccagaccg ccggcacgcc cgtgcccgtc ttcccggtag ggacctgcga
      481 gggcgaatgg cgctccgaga cgtccttgcg cgcctcgctc cagccgcttc tgaacggccc
      541 ggagctctat gccgtatacg gcatgccctc ccagtggcag ccgttgatgg gcgacgcgca
      601 gtacgtggcc gtcttcagcc gcaacgcgcc gggccagagc cgccttgggg ccggcgtcgt
      661 catcgctggc ggccgcatcg cgggcgtgtg gtacggatgc gggtcattcg tcgaccagat
      721 cgtgccggcc ggcacgtcga cgctcctcgg accacgtcgg taaccgcagg tcgtaaggcg
      781 gacctcgggt aaagattcgt gatacgaggc gaattgcgaa cgtggtgctt cgctaatcgc
      841 ttgtcgcgcg cccggcgcgt gcctagaatc gcgccatcga gttgatcctg cggcagcagg
      901 tggaagggac ccgccagtga cctacgtcat cacgcagccg tgtatcgaca cgctcgacca
      961 gtcgtgcgtc gacgtctgcc ccgtggactg catccacttc gacgagggcg tcgaccgcaa
     1021 gctgtacatc gaccccgacg agtgcatcga ctgcggcgcc tgcgagcccg cctgccccgt
     1081 ctccgccatc ttcgccgagg atgacgtccc cgccgaccag gcgatcttca aggagatcga
     1141 cgccctctgg taccaggaca aggacgccgc gagggccaag atccccgcgt agtctcctcc
     1201 gaccccgaat cggaccgagc gagggcgccc acgtggcgcc ctcgtcattt cgcgcgagca
     1261 tctactggtt tcgcctccct ccgcggtcta cgctcgatcg gtgacgatct ttgagaccag
     1321 ggccctcacc aagcgctacg gatcggttgc cgccctcgat gacttcagca tcgaggtcca
     1381 acctggcgtg atcggattgg tcggcgccaa cggcgccggc aagagcacgc tcgtgaagct
     1441 gctgctcggc ctcatcgagc cgaccgccgg cgctgcctct gtgctcggct acgacatccg
     1501 gcgcgagggc agtcacctgc gccagtacgt cggctatatg cccgagcacg actgcctccc
     1561 gctcgacatg tccgccgtcg agttcgtcgc ccatatgtgc cagatgagcg gccttccccg
     1621 cgccgccgcg agggagcgca cggcggagac ccttcggcac gtcggtcttt tcgaggagcg
     1681 ttaccgagcg atgtcgggtt actccaccgg catgcggcag cgcgcgaagc tcgcgcaggc
     1741 gctcgtccac gatccgagca tcgtctttct cgatgagccc acgaacggcc tcgaccccgg
     1801 cggacgcgac gagatgctcg acctcattcg acgcaccggg cacggcctcg gcatcagcat
     1861 cgtcatggcg acgcacctgc tgagcgagat cgaacgggtc tgcgaccagc tcatcgtcat
     1921 cgacgccggc cgcctccagc actacgggcc acttgcggag ttcaccgagg cgacgcagtt
     1981 cctcaccgtc gaggtgaccg ccgatccgga gcccgtcgcg gcggcgctcc gtcgccgcgg
     2041 tctcacctgc gccgtcgaag gccaccacat ttccgtgcgc gtggacgacg gctccgcgcc
     2101 ctacgacctg atccgcgaca cggtcgtcga cctccgggtg gggctggtgc ggatgagccg
     2161 ccgccggcac accctcgagg acctcttcag cgtccggcag gcccaggcgt ccgagccggt
     2221 gaccgcgcgc gaggcggcct cctgatggtg agcacgcccg ggcgggccgc gccaagcaac
     2281 atctacgacc tcggctaccg ccattacgag gggctgcggc tcggccgccg tcacgcgatg
     2341 ttcgtgctct acctcgagag cctccgcggc gcgttcggcc tcggccgcgg ggccgccgcg
     2401 aaggtcgcgc ccgccgcgtt gatcttcatc gccgctattc ccgcgttgat ccagttgctc
     2461 gtcagcgcgc tcttcccggg cgaggccgag gtctttgccc accacgatta ctaccgcgtg
     2521 atctcgttcg tgctcggcct gtactgcgcc gccgtcgccc cggacatcgc gggccgcgac
     2581 cagcgcaacc gctccctcac gctctacttc tcgagggcca tcgaacgcac cgattacgcg
     2641 atcgcgaagc tcgccgccat ggccagcgcc atgctgctga ttacactcgg cccccagctc
     2701 ttactcctgg tggcgaacgg cctcgtcgcc gaggacttca gcacccacct gcgggataac
     2761 tggcacctcc tcttcccgat cgccggctcg gcgctcgtcg gctccgcgct gatcgcctcg
     2821 atggggatcg ccattgcctc gcagacgtcc cggcgcgcgt tcgccacggt gggcatcatc
     2881 gtggcgttca tcgtgccaac agccgtcgcg cgcatcctgg tcgtcgggat cgacaccagc
     2941 gccacgcact acggcgtgtt cgtcagcccc ctcgacgtcc tcggtggcct ttcctcctgg
     3001 atgttccgcc agcccacacc gaggggcggc gcagtcgccg tcgcgggctt cgaaggctgg
     3061 acgtacctcg tcgttgcgct cgtcgtcacg gcggtcgcat ccctgctcgt cctgcggcgc
     3121 tactggtcgg tgcgggcgtg accgccgcga cttccccagt cgacgccgcc cccgccatcg
     3181 aggtcagcga aacctcgcgc tggtacggca acgttgtggc ggtgaacgac atctcgttca
     3241 ccgtgggccc cgggatcacc gggttgctcg gtcccaacgg ggcgggaaaa tccacgctgc
     3301 tccacatgct ggccgggttt ctgcgcccct cctcgggcac ggtccgcatc ctcggcgctc
     3361 ccgcctggca gaacccgggc ctctaccagc aggttggcat cgtccaggaa cgtgagtccg
     3421 tctacgacgg cctgacgggc tacgagtttg tccggctgag cgcgcgcctg catcacctcc
     3481 ccgacgtcga ggcggccaca cgcctcgcga tcgaccgggt gcagatggag accgcgcagg
     3541 gacgcaagac cggcggctac tcaaaaggga tgaagcagcg catcaagatc gccgccgcca
     3601 tcgtccacga ccctcccgtg ctgctcctcg atgagccgtt caacggcacc gaccccgcgc
     3661 agcgtctcca gatgatggac ctcctccgcc gcatggcggc cgagggacgc accattctgt
     3721 tctcgtctca catcctcgag gaggtcgaac ggctggccga gcgcgtgctc gtcatcgtct
     3781 cagggcgtct cgccgcggcg ggggacttcc gcgccatccg ccgtctgatg acggaccgcc
     3841 cgcatacctt cgccatcgtg tcgagcgaca accgcgcgct cgcgagcgcg ctgctcgccg
     3901 acgacagcgt gaccggcgcg cagatcgacg gcgagcgcat caccgtgcgc gccgacgatt
     3961 tcgagcgctt cacggtccac gcacctcgca tcgcgcgctc ggccggcgtc acgatcaccg
     4021 aggtcgacac tctcgacgag tcgctcgaaa gcgtctttga gtacctggtg caacgatgat
     4081 tggcctcatc ttcacgctca cgctcgggca gttgctgcgt caacggcggg tgctgctgct
     4141 cggcctgctc gccgcgatcc cggtcgccat cacgctgctc ttccgcttca ccagcagccg
     4201 cgcggacacc gggcttcccg aattcgtcgc gagcatgctc gacgcgctgg tcgtgagcat
     4261 cggtctgccg ctcacggcgc tggtgctggg tacggccgcc ctcggccagg agatcgagga
     4321 cgggacggcc ttctacctcc tgagcaagcc aatcgcgcgc tggcagatcg tcgtcgcgaa
     4381 gctgatggcc gcctgggtcg ccacctcgat cttcgttacg gtgtcgacgc tcgggtcggc
     4441 cgcgccgctc atcggcgagc gtggcgagga catgctggtg cccgcgttcg taatcgccgg
     4501 aatcgtcggt gcgctcggct atacgaccct attcctggcc ctcagcatcc gcttcaacca
     4561 cgccctcgtg atcgggctga gctacgtgtt cctgtgggag ggactcgctt cgagtctctt
     4621 caagatcgat ggcgtgcgtt tcctcagcct gcgcgcctat accctcggca tcgccggcgc
     4681 aatctccgag gcgccctcga ccgtcttctc agcgcgtctc gatgggccgt ccgcctcagt
     4741 cctggcgacg atcctcatcg tgggcgcctt catctacgcc gtccgtcgcc tcgagcgtta
     4801 cgagttgtcc gagcgcgtct agacgcttcg cgcgccccta cgacccctgc aggaacggga
     4861 tctcgtcgtg cgacaggatc cgcgcgccct cgtcgagggg atgcgaggtc agccgcaccg
     4921 tgccctcgcg ctggaaatcg accacgagga cacgaccgct ttcaccgagg atgctcgcat
     4981 gcccgtcggg tgcacccgaa tacgccgcga gcaggtcgcc ggccgcgatc cgcgtctgct
     5041 gctcccagtg ccctagcgcc acgtagtcgt agtcgaggct cgtgaaatgc tcctcgcgga
     5101 tgtggaacga cgggtggtcg cccgagcgcg ggtgccagta atgcccgtgc cccaccgcga
     5161 tgtgccaggg cgcatcgccg cgcccggggg cgtcgatgaa cggcgagaag tccgggtcat
     5221 gctcgaggtg gctccgcgcc cacagctcca ggtcgagccc ctcgaacgcg accgtctcgc
     5281 cctcgaccga acgcgtgatc gtcaggttcg gcgcgatcga ggtgaggtcg atgcggtcgt
     5341 acaccgagtt cttgcccacg tggtcgtggt ttcccggcac gatcacggtc ggcacctcga
     5401 tgcgcgcgat ctgctccgcg gcgaaacgca gtgtctcctc gctcacccgc gcattgtcga
     5461 agaagtcgcc cgcgatgacg aagacatcga cccgttccgc gatcccgacg tccaccacgc
     5521 gccggaagcg gtcctcggac tgctcgcgac gctcgtccca ccgtccgctg cggtcgaagt
     5581 cgaacgtccc gagatggacg tccgaggtgt gcagcagacg gaacgggcgt cgcgcctcgg
     5641 tcatcgtccg atcatccctc ggccctccgc agggcatcga tgcgctcctc gatcgccgcg
     5701 cgcagcccgt cgatcggcac ccgcacctgg ctcatcgagt cgcgctcacg aatcgtcacc
     5761 gcacggtcct cgatcgtctc gaagtcgacc gtgacgcaca gcggcgtccc gatctcgtcc
     5821 tgacgccgat agcgccgccc gatcgactga gcgtcatcgt actgcgtgcg gaaatgcggt
     5881 cgcaggaggt cgtacacctc gcgcgccgtt ggcgtcagtt gctcattgcg cgagagcggc
     5941 aggatcgcca ccgtcaccgg cgccacgagg ggcgacagat gaaggacgat ac
//
LOCUS       DAORPG010000033         5945 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_714_length_5945_cov_3.390323, whole genome shotgun sequence.
ACCESSION   DAORPG010000033 DAORPG010000000
VERSION     DAORPG010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_714_length_5945_cov_3.390323"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PLX85_01435"
     CDS             <1..684
                     /locus_tag="PLX85_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC61870.1"
                     /translation="VHDFDGTRADKTHGLLELQRDYIRRGFNVLAFDLRGRGESGGVR
                     DYLGAQERKDLPAAVEYARSRAPMLPVVIHGFGLGGALAVVAVADGSVEADAVVADST
                     FTSVRAYLRRRWAVVPWPLFQLACYFARRLYQADADAIAPLAAMRATSTLTPMLFIHG
                     ERDRLISVTHTINIAASSLNARTSVYRVPRAGHCTGYLRGPEAYVRHCLDFVDGALPA
                     RRISVAAAG"
     gene            706..1719
                     /locus_tag="PLX85_01440"
     CDS             706..1719
                     /locus_tag="PLX85_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005581764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HRC61871.1"
                     /translation="MPLRDGITDVPGIRVGHWTDRRAATGCTAVLCSAGAVGGADIRG
                     AAPGTRETDLLRSENLVEEVHAVLLTGGSAFGLDAASGVVRYLREQESGLRFGEFIIP
                     IVPAAVLFDLNVGRESWPNADAGYLAANRARGGRVVEGSVGAGTGATVAKFAGPDRRL
                     KAGLGTASEAFGKGLVVGAIVAVNAVGLVRDPTTGETVAGPRGSEGEFLDAEAFLRGL
                     GSGSSDGDEDPPAVGSNTTLAVVATNAMLTKTQANRLATVCHDGFARAIWPVHTRGDG
                     DAVFTLATGERDLEGPAYLALEAFATRAVERAIVRGVQCARGLARVPSASEWLGAAPS
                     ATH"
     gene            1954..2361
                     /locus_tag="PLX85_01445"
     CDS             1954..2361
                     /locus_tag="PLX85_01445"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013288959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRC61872.1"
                     /translation="MRMMLHGKIHRATVTQADVDYIGSIAIDETLMDAAGIIEWEQVD
                     VLDITNGARLTTYAIPAPAGSGMICPNGAAAHLVHPGDLVIIVAYEGVATADLASHRP
                     NIVHVDAQNRIVDLARSDAELTQTIAAREGASR"
     gene            2358..3272
                     /gene="panB"
                     /locus_tag="PLX85_01450"
     CDS             2358..3272
                     /gene="panB"
                     /locus_tag="PLX85_01450"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRC61873.1"
                     /translation="MKRVSVTDLQKMRDDGRRITMLTAYDYHTARLADEAGIPILLVG
                     DSLGMVVLGYESTLPVTVDDMIHHGKAVVRGAQRAHVVVDMPFGSYQGGWEDAMRNAV
                     RIMQETGAGSVKLEGGVRSAELVRRLVDAGIPVMGHVGLTPQSVNAFGGWKIQGKTPR
                     DGVRVMADAKAIEEAGAYAVVLETVPAMLGAKITQQLRVPTIGIGAGPYCSGQVQVVH
                     DLLGLDELKPKHARRFLQGGDLLRSAFATYREAVESGAFPTAAESHLLDEQVVRRIER
                     MASYSFPTNMQHEGIDLEGALAESEAEG"
     gene            3281..4120
                     /gene="panC"
                     /locus_tag="PLX85_01455"
     CDS             3281..4120
                     /gene="panC"
                     /locus_tag="PLX85_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRC61874.1"
                     /translation="MQVVRTVAEFREARRRLPAPVGFFPTLGYMHEGHISLVERARRE
                     CASVVASIFVNPTQFGPNEDLSRYPRDEARDLAMLEGAGVDLVILPSVEEMYPAGDVT
                     RVRVPGLSEVLEGERRPGHFEGVTTVVAKLFGITEADFAYFGQKDAQQLLIIERMVRD
                     LHMPVTVVRCPTVREADGLACSSRNVYLSPEERAQAVALWAGLRAAEAACGAGERDTD
                     RLRAVCREQIEARPLASIDYLSLADEQTLEELHGLVERPALMSLMVSFGNTHLIDNIT
                     LHP"
     gene            4206..4958
                     /locus_tag="PLX85_01460"
     CDS             4206..4958
                     /locus_tag="PLX85_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRC61875.1"
                     /translation="MSGHSKWSSIKHKKGALDAKRGQLFTKLARDITMAARGGGSGDP
                     SMNAPLRLAIARAKDNNMPNDNIERAIQRGTGGGDTDTLIEITYEGYGPGGTAVIVEA
                     VTDNRNRTVAEVRAAFSRGGGNLGESGAVAWQFDTRGVITVEAKGTDPDEVQLTAIEA
                     GALDISVDEDEVEVLTEPADLEAVREALTSSGLKVAQAEISRVPRTLISLDEKAAIQT
                     LKLLERLDDLDDVSRVYSNADFPDEVLAAADA"
     gene            4969..5469
                     /gene="ruvC"
                     /locus_tag="PLX85_01465"
     CDS             4969..5469
                     /gene="ruvC"
                     /locus_tag="PLX85_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRC61876.1"
                     /translation="MRRVLGIDPGLATTGYGVLEGDGVHARVIAAGALRTRARDSRAA
                     RLASLFEALTGLLEEHRPQDMALEQHFVANNVRSAMAIGEARAAAMIAAARARVEVYE
                     YPPATVKESVCGFGSAGKEQVQAMVRMHLGLAELPEPLDVSDALAVALTRLAGYRMDA
                     LLAASK"
     gene            5466..>5945
                     /locus_tag="PLX85_01470"
     CDS             5466..>5945
                     /locus_tag="PLX85_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HRC61877.1"
                     /translation="MIVALRGALTRWDEERSALWIEAGGVTYEVAMPAFAGDWVGGHA
                     VGDEVQVFTYEYASERQPAPTLFGFPRLAEREFFRKFIEVPDIGPAKAVRALTRPVSD
                     IARWVEAEDARALRQLPGIGDRLAQTIIAHLRGKLVQEALLQDRDGHTPVAAPDIRAD
                     "
BASE COUNT          951 a         1955 c         2094 g          945 t
ORIGIN      
        1 gtccacgact tcgacggaac gcgggccgac aaaacccacg gcctgctcga actgcagcgc
       61 gactacatcc gccgcggctt caacgtcctc gcattcgacc tgcgcggacg cggtgagtcg
      121 ggcggcgtcc gtgattacct cggcgcccag gagcgtaagg acctgcctgc ggctgtggag
      181 tacgcccgat cgcgggcgcc gatgctgccg gtcgtgatcc acggcttcgg gctcggagga
      241 gcactggccg tcgtcgcggt tgcggacggg agcgtcgagg cggatgcggt cgtcgccgac
      301 tcaacgttta cgtcggtgcg ggcctacctg cgccggcgct gggcggtggt gccgtggccg
      361 ctgttccagc tcgcgtgcta cttcgcgcgg cggctgtatc aggccgacgc cgatgcgatc
      421 gcgccgctcg cagcgatgcg cgccacgagc acgttgaccc cgatgctctt catccacggt
      481 gaacgagacc gactgatctc ggtaacacac accatcaaca tcgccgcctc ctcgctgaac
      541 gctcgcacga gcgtctaccg cgtgccgcgc gccggccact gcactggcta cctgcgcggt
      601 cccgaggcat acgtccgcca ttgcctcgac ttcgtcgacg gcgcgttgcc ggcgcgccgc
      661 atcagcgtgg cggcggcggg ctagcccgct ccgcgacgac cttcaatgcc gctgcgggac
      721 ggcatcaccg acgtccccgg gatccgcgtg ggccactgga ctgaccggcg cgctgcgacc
      781 gggtgcaccg ccgtcctgtg ctcggcgggc gcagtgggcg gggccgatat caggggcgcc
      841 gcgccgggca cgcgcgagac ggacctgttg cggtccgaga acctggtcga agaagtgcat
      901 gccgtcctgc tcaccggcgg gtcggctttt ggtctcgatg cggccagcgg tgtcgtgcgg
      961 tacttgcgcg agcaggagtc cgggctgcga ttcggcgagt tcatcatccc gatcgtccct
     1021 gcggcggtgc tgttcgacct gaacgtcggc cgtgagtcat ggccgaacgc cgatgccggc
     1081 tacctcgccg cgaaccgcgc tcgcggtggg cgggtagtag aaggttcggt gggcgcgggg
     1141 accggggcga ccgtcgcgaa gttcgcggga cccgaccgcc gcctgaaggc aggccttgga
     1201 accgcgagtg aagcgttcgg taagggactc gtcgtcggcg cgatcgtggc cgtgaatgcg
     1261 gtaggtctcg ttcgcgaccc cacgacgggc gagaccgtcg ccggcccgcg aggcagcgaa
     1321 ggcgaattcc tggatgccga agcgtttctt cgcggactcg ggtccgggtc ctcagatggc
     1381 gacgaggacc cgccggccgt gggctcaaat acgacgctcg cggtggttgc gacgaatgcg
     1441 atgctcacca agacgcaggc gaatcgtctc gccacggtgt gtcatgacgg gttcgcccgt
     1501 gcgatctggc ccgttcatac ccgcggtgac ggcgatgcag tgttcaccct cgcaaccggc
     1561 gagcgcgacc tggaggggcc ggcgtacctg gcgctggagg cgttcgccac tcgcgcagtc
     1621 gaacgcgcga tcgtgcgcgg agtccagtgc gcgaggggcc tcgcgcgggt gccgtcagcc
     1681 tccgagtggc ttggcgccgc gccgagcgcg actcattgac aggcataatc aggtacctat
     1741 actgaaggcg cccggtcgac cgcacggcgg ccaggggacg gtcagagacg gaaacgagcc
     1801 cgcagaaacc gcttggatcg agagaccgag acggagggta ggtatggttg attgcgttca
     1861 tgcacctcgg ctttgccggg gtgttgctgt atctgaggca atgggcccgc ggccgatgat
     1921 gcctcggatg atgctgtcag ggggtgagca ccgatgcgga tgatgctcca cggaaagatc
     1981 caccgcgcca ccgtcaccca ggcggacgtg gactacatcg gctcgatcgc catcgacgag
     2041 acgttgatgg acgccgccgg catcatcgag tgggagcagg tggacgtcct cgacatcacg
     2101 aacggcgcga ggctgacgac ttacgctatc ccggccccgg cggggtccgg catgatttgc
     2161 ccgaatggag ccgcggcgca tctggtgcac cccggtgacc tcgtgatcat cgtcgcctac
     2221 gagggcgtgg ccacggcaga tctcgcctcg caccgcccga acatcgtgca cgtcgatgca
     2281 cagaaccgca ttgtcgacct ggcccgcagc gacgccgagc tgacgcagac catcgctgca
     2341 cgggaaggag cgtcgcgatg aagcgagtgt ccgttaccga cctgcagaag atgcgcgacg
     2401 acggccgtcg catcaccatg ctgaccgcct acgactatca caccgcgcgc ctcgcggacg
     2461 aggcgggcat cccgattctc ctcgtgggcg actcactcgg gatggtggtc ctcggctacg
     2521 agtcgacgct gccagtcacc gtcgacgaca tgattcatca cggcaaagcc gtcgtccgtg
     2581 gcgcccagcg cgctcacgtc gtcgtcgaca tgccgttcgg ttcgtaccag ggcggctggg
     2641 aagacgcgat gcgcaatgcc gtgcgcatca tgcaggagac gggcgccggc tccgtgaagc
     2701 tcgagggcgg cgtccgttcc gccgagttgg tgcgacggct cgtggatgcc gggattcccg
     2761 tcatgggtca cgtcgggctg acgccccagt cggtgaacgc gtttggcggc tggaagatcc
     2821 agggcaagac cccacgcgat ggcgtacgcg tcatggccga cgccaaggcc atcgaggagg
     2881 cgggcgccta cgcggtggtg ctcgaaaccg taccggcgat gctcggcgcg aagattacac
     2941 agcagctgcg ggtgcccacg atcggcatcg gtgcggggcc gtactgcagc ggccaggtgc
     3001 aggtcgtcca cgacctcctc ggcctcgacg agctgaagcc caagcatgcc cggcgctttc
     3061 tgcagggcgg cgacctgctg cgctcggcct tcgccaccta tcgcgaggcg gttgagtcgg
     3121 gcgccttccc gacggcggcg gagtcccacc tgctcgacga gcaggtggtg cgccggatcg
     3181 agcggatggc gagctactcg ttcccgacga acatgcagca cgagggcatt gacctcgaag
     3241 gcgcgctcgc cgagagcgaa gccgagggct agcagggacg gtgcaggtcg tccggacggt
     3301 cgccgagttc cgggaggcgc ggcgccgcct ccctgcgccg gtgggattct ttcccacgct
     3361 cggctacatg cacgaggggc atatctcgct ggttgagcgc gctcgacgtg aatgcgcgag
     3421 cgtcgtcgcc tccatcttcg tcaatccgac ccagttcgga ccaaacgagg acctcagtcg
     3481 ctatccgcgg gacgaggcac gcgacctcgc gatgctcgag ggcgccggcg tcgacctcgt
     3541 gatcctgccg tcggtcgaag agatgtaccc cgcgggcgac gtgacgcgag tccgagtccc
     3601 gggactgagc gaggtgttgg aaggggagcg ccgtcccgga cacttcgagg gcgtgacaac
     3661 cgttgtcgcc aagctgttcg gcattacgga ggctgacttc gcctacttcg gccagaagga
     3721 cgcgcagcaa ctgttgatca tcgagcggat ggtgcgcgat ttgcacatgc cggtgactgt
     3781 cgtgcgctgc ccaacggtcc gcgaggcgga tggtcttgcc tgctcgtctc gcaacgtata
     3841 cctctcgccc gaggaacggg cgcaggctgt cgccctctgg gcgggcctcc gcgccgctga
     3901 agcggcctgc ggggccgggg agcgcgacac ggatcggctc cgggcagtct gccgcgaaca
     3961 gatcgaggcg cgtccgctcg catcgattga ctacctctcg ctcgccgacg agcagacgct
     4021 cgaagagctc cacggcctcg tcgaacggcc cgcgctgatg tcgctgatgg tgtcgttcgg
     4081 gaatacgcac ctgatcgaca acatcacgct tcatccgtaa gcccctgcct gagcggcggc
     4141 ctcgcgcgtt cgttccgcac gtcgacgtcc gctggtagga tgaaatcgca gtcaggagag
     4201 cgcgcgtgtc cggtcattcc aagtggtctt ccatcaagca caagaagggc gcgctggacg
     4261 cgaagcgcgg ccagctgttc acgaaactcg cccgcgacat cacgatggcg gcgcgcggcg
     4321 gcggcagcgg tgacccctcg atgaatgcgc cgctgcgtct tgcgatcgcg cgtgccaaag
     4381 acaacaacat gcccaacgac aacatcgagc gcgcgatcca gcgcggtacc ggtggtggcg
     4441 acacggacac gctcatcgag atcacgtacg agggctacgg ccccggcggc acggccgtca
     4501 tcgtcgaggc cgtgaccgac aaccggaacc gcacggtcgc tgaggtacgc gcggcgttct
     4561 cacgaggcgg tggcaacctc ggcgagagcg gcgccgtcgc gtggcagttc gatacgcgtg
     4621 gcgtgatcac cgtcgaggcg aaggggaccg accccgatga ggtgcagctg acggccatcg
     4681 aagccggcgc cctcgacatc tcggtcgacg aggacgaggt tgaggtgctc actgagccgg
     4741 cggatctcga ggcggtacgc gaggcactga cgtcgtctgg cctcaaggtc gcacaggcgg
     4801 aaatctcgcg cgtgccgcgc acgttgatct cgctcgacga gaaggccgcg atccagacgc
     4861 tgaagctcct cgagcggctc gacgacctcg atgacgtgtc gcgcgtctat agcaatgcgg
     4921 acttccctga cgaggtgctg gccgcggcgg acgcctagga tggcgccggt gcgccgggtg
     4981 ctgggcattg accccggcct ggcgacgacc ggctacggag tcctggaggg ggacggcgtg
     5041 cacgcccgcg tgatcgcggc gggtgccctc cgcaccaggg cgcgggacag ccgtgccgcc
     5101 cgtctcgcct cgctgttcga ggccctcacg ggactcctcg aggaacaccg accgcaggat
     5161 atggcgctcg aacagcactt cgtcgcaaac aacgtgcgct ccgcgatggc aattggcgag
     5221 gcgcgggcgg cggcaatgat cgccgcggcg cgtgcccgtg tcgaggtgta cgagtatccc
     5281 ccggccaccg taaaagagag cgtctgcggg ttcgggagcg ccggcaagga acaggtgcag
     5341 gccatggtcc gcatgcacct tgggctcgct gagctgccgg agccgctcga cgtgtcggac
     5401 gccctcgccg tcgccctcac gcggctcgcg ggttatcgga tggacgcgtt gctggcggcc
     5461 tcgaagtgat cgtcgcgctg cgcggcgcac tcacgcgttg ggacgaagag cgttccgcgc
     5521 tctggataga ggccggcggc gtgacgtacg aggtcgcgat gccggccttc gccggcgact
     5581 gggtgggcgg tcacgcggtt ggcgacgagg tgcaggtctt cacctacgag tacgcaagcg
     5641 agcggcagcc cgctcccacg ctctttggct tcccgcgcct tgcggagcgc gagttctttc
     5701 gcaagttcat cgaggtgccg gatattggcc cggcgaaggc cgtccgtgcg ttgacgcgac
     5761 cggtctctga catcgcgcgc tgggtggagg ccgaggacgc gcgcgcactc cgtcagttgc
     5821 ccggtattgg cgaccgactg gcacagacca tcatcgcgca cctccgcggc aagcttgtgc
     5881 aggaagcgct gctgcaggac cgcgatggcc acacacccgt cgccgcgccc gacatacgtg
     5941 cggat
//
LOCUS       DAORPG010000034         5884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_733_length_5884_cov_3.336250, whole genome shotgun sequence.
ACCESSION   DAORPG010000034 DAORPG010000000
VERSION     DAORPG010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_733_length_5884_cov_3.336250"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLX85_01475"
     CDS             <1..374
                     /locus_tag="PLX85_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate transporter"
                     /protein_id="HRC61878.1"
                     /translation="AAESGVMLHLAIPAVDAGPVAAYCRYPIHGPAFDAAWDALGDNT
                     LQDDGAIEASPLFAAIRAAGVARESPLLVATLQAFADGRLVVRDGQVFDSAGQPAAAA
                     DLTAEVEAIVASSADAPGIPR"
     gene            475..729
                     /locus_tag="PLX85_01480"
     CDS             475..729
                     /locus_tag="PLX85_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61879.1"
                     /translation="MSAPPVLVPLTSRLPLRRDGPLLPSPLRALPVAGGIAGVALAGL
                     ATEYVLRAAAVHVIETLIRRPAPKRAVVTEVTVIERFRRD"
     gene            732..2231
                     /gene="cmk"
                     /locus_tag="PLX85_01485"
     CDS             732..2231
                     /gene="cmk"
                     /locus_tag="PLX85_01485"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRC61880.1"
                     /translation="MTRERLQKILARAGHGSRRSAESLIAEGRVEIDGKRAALGDQAD
                     PAFQRITVDGRPLEVRAPSGTWMLHKPVGYVVTASDERGRPTVYTLVPPEAASTRYVG
                     RLDIDTSGLLLLTTDGELAHRLTHPRYEVWKTYEAEVVSTPDARSLDLLRNGVPLDDG
                     VTAPARVRVLRHGPRPLVELQLREGRKREVRRMLAAVGAPVRRLRRVAFGDVRLGDLR
                     AGAMRRLTPQEEHGLRRLVGMPVEVDADEGGSLSSSGPLAADPAARERPPISTDSLAR
                     SIAIDGPTASGKSVVGRALAEHLGLGFVDTGLMYRACTLAVLERGIDPNDAAAVTALV
                     RGLDLDLQWPDPSRPRATLDGVDVTDRLRQPEIERNVSLISRIAEVRDELVQRQRRLA
                     SRSPIVMAGRDIGTRVLTEARTKVFLEASTEIRARRRMGEELDAGRNTTFERVLDETR
                     TRDELDSTGKRAIRRDQAAPDAIVIDTDMLGIDEVVEVCLNAYRDANGT"
     gene            2228..2941
                     /locus_tag="PLX85_01490"
     CDS             2228..2941
                     /locus_tag="PLX85_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRC61881.1"
                     /translation="MKSLLYRLVSGWIYGFLTGLTRVVVYTFARWTVEGAEHIPTTGP
                     VVLVSNHLHLLDPPLLAACSKRRVHPMAKRELFETPLVGWVLWPYGAFPVRRFSADMG
                     ALRAARGYLRNGHVVLMFPEGTRSKDARLHPALPGAAMVALLADAPVVPVAITGTESI
                     RVPGTLFRWMRRDRLRVHVRFGEPFHLEFESAEARHAERATDLIMRRIAALLPDEYRG
                     AYGPESEGQIVFARQPQDA"
     gene            3031..4875
                     /gene="glmS"
                     /locus_tag="PLX85_01495"
     CDS             3031..4875
                     /gene="glmS"
                     /locus_tag="PLX85_01495"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRC61882.1"
                     /translation="MCGIIGYTGQRPAGQIILDGLRRLEYRGYDSAGIALFDANHELY
                     VARAAGKIDNLISKIEGSLPPAVSGIGHTRWATHGAATDLNAHPHLDPAGRVAVVHNG
                     IIENFEALRRPFVERGLAFSSDTDTEVLAFLIADEIQAGVDLARAVQNVVAQTQGAAA
                     VVAICCDEPGRIVAARVGNAGGIVIGHGDGERFVASDLAALLPHTRRVTFLEPGEVAD
                     IRPDEVTYYDTAGHVIAKPVETQPWDPVAAVKGRYKHFMLKEIHEQPDTALDSVRGRY
                     LTAPHRVEVEDLPFTAEQVRDFNRVVLIGMGTSMHAAMVGRHYLERYARLPAEVDNAA
                     EFRYRSPVLDPRTLVISVAQSGETIDTLAAMDEARRAGCPQITICNTPGAQTTRIADA
                     TVYMRAGPEIAVASTKTLVGSMIALHALGLHLGRLRGALDAETSRRQVEGALHLPAAI
                     GETLLLEPRVAELAARYAAYEDFLFLGRGLSYPMALEGALKLKEVSYIHAEGYAAGEM
                     KHGPIALIDERMPTVAIALRDEVRDKMRSNIEQVKARRGRVIAIVSADDVSLDGIADD
                     VLRLPEVEPDLSPVLTAVVMQLLSYEIAVERGADVDQPRNLAKTVTVE"
     gene            4950..5177
                     /locus_tag="PLX85_01500"
     CDS             4950..5177
                     /locus_tag="PLX85_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61883.1"
                     /translation="MPARHGHDEHCRELHRALDRAKAMLRTAPIRETLGGDPHAAVAE
                     LRSEREASTTAEIARIEQALREHGCTLSRIN"
     gene            complement(5184..5879)
                     /locus_tag="PLX85_01505"
     CDS             complement(5184..5879)
                     /locus_tag="PLX85_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61884.1"
                     /translation="MPSHAARLTARPATGRTSIESGVTRVQQSNGRVGYLYVPPDHLE
                     PCPLMVLFHGAAQFAARSLEFLQEPASAAGAALLIPQSLGRTWDIIEGGFGPDVQALD
                     EALSWAFGVLDVDPTHLGSAGFSDGGSYALSVGLVNGDLFSHVVGWSPGFVSAPVRHG
                     RPPVFISHGTRDPVLPIDHCSRRIVAQLRTEAYQLDYREFDGLHILPPEIADEAFAWF
                     LRGRGESGVAPVE"
BASE COUNT          937 a         2149 c         1930 g          868 t
ORIGIN      
        1 gcgctgccga gagcggagtg atgctccacc tggccatacc ggcggtggat gcgggccccg
       61 tggccgccta ctgtcgctac ccgattcacg gccccgcctt cgacgcagca tgggacgcgc
      121 tgggcgataa cacgctgcag gacgatggcg cgatcgaggc aagtccgctc ttcgccgcga
      181 tccgagccgc cggcgtcgcg cgtgagtcgc cgctcctggt ggccaccctc caggcgttcg
      241 cggacggacg tctcgtcgtg cgtgacgggc aggtattcga ttcggccggg cagccggccg
      301 cggcagcgga cctcacggcg gaggtggaag ccatcgtcgc cagcagcgct gacgctcccg
      361 ggatcccgcg ctgatcgggc gtgcggaacc cgatactcgg gtcaccgagg tcgctgctgg
      421 ctgagtgcgg taccatcgtg ccagtgtcgc ccgcatgaac tcgacaggcc ccacatgtcc
      481 gcccctccgg tcctggtccc gctgacctcg aggctaccgc tgcgtcgcga cgggccgctg
      541 cttccctcac cgctgcgcgc acttcccgtc gccggaggca tcgccggtgt cgcactggcc
      601 ggcctcgcca ccgagtacgt cctgcgggcg gcggccgtcc acgtgatcga aacgctgatc
      661 cggcgccctg ccccgaagcg ggccgtggtc accgaggtga ccgtcatcga gcgcttccgc
      721 cgcgactgaa catgacgcgc gagcgcctgc aaaagatcct cgctcgagcc ggacacggca
      781 gccggcggag cgccgaatcc ctgatcgccg agggccgcgt cgagatcgac ggcaagcgcg
      841 ccgcactcgg tgaccaggcg gacccggcgt tccagcgcat cacagtcgac ggccgcccgc
      901 tcgaagtccg cgcgccgtcc gggacatgga tgctgcacaa gcccgtgggt tacgtcgtca
      961 ccgcatccga cgagcgcggc cggcccaccg tctacaccct cgtccccccc gaggcggcca
     1021 gcacgcgtta cgtcggccgg cttgacatcg acacctccgg gttgctgctc ctgaccaccg
     1081 acggcgagct cgcacatcgg ctcacgcacc cccggtacga ggtgtggaag acgtacgagg
     1141 cggaggtcgt ctcgaccccc gatgcgagga gcctcgacct gctccgcaac ggcgtcccgc
     1201 tcgatgacgg ggtaaccgcg ccggcccgcg tccgcgtgct tcgtcacggc cctcgaccgc
     1261 tggtcgagct gcagctgcgc gaaggccgca aacgagaggt gcgtcggatg ctcgcggcag
     1321 tcggcgcccc ggtccgtcgg ctgcgccgtg tggccttcgg cgacgtgcgg ctcggcgacc
     1381 tgcgggccgg agcgatgcga cgactgacgc ctcaggagga gcatggactc cgccgcctcg
     1441 tcggcatgcc cgtcgaggtg gacgctgatg aaggaggctc cctatcatcg tcaggcccgc
     1501 tcgccgctga ccccgctgcc agagagaggc ccccgatctc caccgattcg ctcgcccgca
     1561 gcatcgccat cgacggaccg acggcttcgg gcaagagcgt cgtcggccgc gcgctcgccg
     1621 agcacctcgg cctcggcttt gtcgacaccg ggctcatgta ccgtgcctgc accctggccg
     1681 tcctcgagcg cggcatcgac ccgaacgatg cggccgccgt caccgcgctc gtccgtgggc
     1741 tcgacctcga cctgcagtgg cccgacccct cccggcctcg tgcgacgctc gacggcgtcg
     1801 acgtaaccga ccgcctgcgt cagcccgaga tcgaacgcaa cgtctcgctc atctcgcgca
     1861 tcgccgaggt gcgcgacgag ctcgtgcaac gccagcggcg gctcgcctct cgatcgccca
     1921 tcgtgatggc aggccgcgac atcggcacgc gagtcctcac cgaagcgcgt accaaggtct
     1981 tcctcgaggc ttcgaccgag attcgggcgc gccgtcgcat gggcgaggag ctcgatgcgg
     2041 gccgtaacac gaccttcgag cgcgtgctgg acgaaacgcg tacgcgcgac gagctcgatt
     2101 ccacgggcaa gcgcgcgatc cggcgcgatc aggcggcgcc tgatgccatc gtgatcgata
     2161 ccgacatgct cggcatcgac gaggtcgtcg aggtctgcct gaacgcttac cgcgacgcca
     2221 acggtacgtg aagtcgctgc tctaccgtct ggtctccggc tggatctacg gctttctgac
     2281 cggcctcacc cgcgtggtcg tctacacctt cgcccgttgg accgtcgagg gcgctgagca
     2341 catcccgacg accggccccg tcgtactcgt ctcgaaccac ctgcatctcc tcgatccgcc
     2401 gttgctcgct gcctgcagca agcgtcgcgt gcaccccatg gccaagcgcg aactgttcga
     2461 gacgcccctc gtgggctggg tgctgtggcc ctacggcgca ttccccgtgc ggcgcttctc
     2521 ggcggacatg ggcgccctgc gcgccgcgcg tggctacctg cgcaacggcc acgtcgtcct
     2581 catgttcccc gaggggacgc gctcgaagga tgctcgcctc catccggcgc tgccgggcgc
     2641 cgccatggtg gcattgcttg cggatgcgcc ggtcgtgcct gtcgccatca cgggcaccga
     2701 gtccatccgc gtacccggca cgctcttccg ctggatgcgc cgcgaccggc tgcgcgtcca
     2761 cgtgcggttc ggcgagccgt tccacctcga attcgagagc gccgaggcgc gccatgccga
     2821 gcgcgccacc gacctgatca tgcgccgcat cgccgccctg ctaccggatg aataccgcgg
     2881 ggcatacggg cccgagagcg aagggcagat cgtcttcgca cgccagccgc aagacgccta
     2941 gcccgcaagc ccacgcggac gaggaacgcg aatcgtccgc cggtcgttcc agacgtacgg
     3001 taacctagag gcgcggggcg gagagagcgt atgtgcggaa tcatcggcta caccggccag
     3061 cgccccgctg gccagatcat cctcgatggc ctgcggcgac tcgaataccg cggctacgat
     3121 tccgccggca tcgcgttgtt cgacgcaaac cacgagctat acgtggcccg cgcggcgggc
     3181 aagatcgaca acctgatcag caagatcgag ggatccctcc cgccagcggt ctctggcatc
     3241 ggccacacgc gctgggccac ccacggcgcg gccacggacc tgaacgcgca cccccacctc
     3301 gatcccgccg gccgcgtcgc ggtcgtccac aacgggatca tcgagaactt cgaggcgcta
     3361 cggcgaccct tcgtcgagcg gggcctcgca ttctcctcgg acaccgacac cgaggtgctc
     3421 gcgtttctga tcgccgatga aatccaggcc ggagtcgacc tcgctcgcgc cgtgcagaac
     3481 gtcgtcgcgc agacccaggg cgcggcggcc gtcgttgcca tctgttgcga cgagccgggg
     3541 cgcatcgtcg cggcccgcgt gggcaatgcc ggaggcatcg tcatcggtca cggggacggc
     3601 gaacgctttg tcgcctcaga cctcgccgcg ctgctcccgc atactcggcg ggtcaccttc
     3661 ctcgaaccgg gtgaggtggc cgacatccgc cccgatgaag tgacgtacta cgacaccgcc
     3721 gggcacgtga tcgcgaagcc ggtcgagacg caaccatggg atccggtcgc agccgtcaag
     3781 ggtcgctaca agcacttcat gctcaaggaa atccacgagc agcccgacac tgcgctcgat
     3841 tccgtgcgcg ggcggtacct gaccgccccc caccgcgtcg aggtggagga cctgccgttc
     3901 accgccgagc aggtacgcga cttcaaccgc gtcgtgctga tcggcatggg cacatcgatg
     3961 catgcggcga tggtgggcag gcattacctc gaacgctacg cgcgcctccc cgccgaggtc
     4021 gacaacgccg ccgagttccg ctaccgcagc ccggtcctcg acccccgcac gctggtgatc
     4081 tcggtcgcac agtcgggcga gaccatcgac acgctcgccg ccatggacga ggcgcggcgc
     4141 gccggctgtc cgcagatcac gatctgcaac acgccgggcg cccagacgac acggatcgcg
     4201 gacgccacgg tgtacatgcg cgctggccca gaaatcgccg tggccagcac gaagacgctc
     4261 gtcggctcga tgatcgcgct gcatgcgctc ggactgcacc tcggccggct gcgtggcgcc
     4321 ctcgatgcgg aaacctcgcg gcgacaggtc gagggggccc tccatttgcc ggcggccatc
     4381 ggcgagacgc tgctcctcga gccacgcgtg gctgaactcg cggcccgcta cgccgcctac
     4441 gaggacttcc tcttcctcgg ccgcggactg tcgtacccca tggcgctcga gggagcgctc
     4501 aagctgaagg aagtctccta cattcacgcc gagggatatg cggccggcga gatgaaacac
     4561 ggcccaatcg ccctcatcga cgagcgaatg cccacggttg ccatcgcctt gcgcgatgag
     4621 gtccgcgaca agatgcgctc caacatcgag caggtgaagg cgcgacgggg acgcgtcatc
     4681 gccatcgtct cggcagacga tgtgagcctc gacggtatcg ccgacgacgt gctgcggctc
     4741 ccggaggtcg agcccgatct cagcccggtg ctcacggcgg tcgtgatgca gttgctctcg
     4801 tacgagatcg cggtcgagcg cggcgcggat gtcgatcagc cgaggaacct cgccaagacc
     4861 gtcaccgtcg aatagctggc aggttcctcc caggcgccgc gagcgcgttc cccatcgtgt
     4921 taacatatgt aatgcgaagg gaggcgagta tgcccgcccg acatggacac gacgagcatt
     4981 gccgcgagtt gcatcgagcc ctcgaccgcg ccaaggccat gctccggacc gccccgatcc
     5041 gcgaaaccct gggcggcgat ccgcacgccg cggtcgccga gctgcgctcg gagcgcgagg
     5101 caagcacgac ggcggaaatc gcccgcatcg aacaggctct gcgtgagcac ggttgcacgc
     5161 tcagccgcat caactgaccc ggctcactcg acgggcgcga cgccggactc gccgcgcccg
     5221 cggaggaacc aggcgaacgc ctcgtctgca atctcaggcg gcaggatgtg taggccgtca
     5281 aattcccggt agtcgagttg atatgcctcg gtccgcaact gcgccacgat tcggcggctg
     5341 cagtggtcaa tgggcagcac tgggtcacgc gtcccgtgtg agatgaacac gggcggccgc
     5401 ccgtgccgca ccggcgccga tacgaaccct ggcgaccagc cgacgacgtg gctgaagagg
     5461 tcgccgttca cgaggcctac cgagagcgcg taggacccgc cgtccgagaa ccccgctgac
     5521 ccgaggtgcg tcggatccac gtccagcacg ccgaatgccc aggacagcgc ttcgtcgagc
     5581 gcctgcacgt ccggtccgaa gccgccctcg atgatgtccc aggtgcgacc gagcgactgc
     5641 gggatgagca gcgccgcgcc cgcggcactc gccggttcct gcaggaactc gaggctccgc
     5701 gccgcgaact gcgcggcgcc gtgaaacagc accatcagcg ggcacggctc gaggtggtcc
     5761 ggggggacgt acaggtagcc gacgcgtccg ttcgactgct gcacgcgcgt cacgcccgac
     5821 tcaatcgagg tacgcccagt cgcgggtcgg gccgtcagcc tcgcggcgtg cgacggcacg
     5881 accg
//
LOCUS       DAORPG010000035         5807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_756_length_5807_cov_3.374305, whole genome shotgun sequence.
ACCESSION   DAORPG010000035 DAORPG010000000
VERSION     DAORPG010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_756_length_5807_cov_3.374305"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="PLX85_01510"
     CDS             complement(<1..924)
                     /locus_tag="PLX85_01510"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC61885.1"
                     /translation="MSQLHGPALVTGSSGFVGGHLVARLVAEGCEVSGLDWVPPRTPR
                     PSEMRDFRVDIRDADAVRRAVLEARPEVVYHLAAQASVSVSMREPEVDIETNVLGSVH
                     LMRAAIEAGTKRFVFVSTGGALFGEPAVVPVTEEMPAAPESVYGASKFAAEQYLRLLA
                     GPGGIELSVVRPGNIYGPAQDPHGEAGVVAIFALRMLANTEATIFGDGSQRRDYVYVE
                     DVVEAALRAATDQPATCLIGTGIGTSTQQIFEHLARLSGYERAPIYGPERSGDIQRIM
                     LDASRAREVWGWQPQTDLETGLAATVRWFRET"
     gene            complement(987..2345)
                     /gene="mgtE"
                     /locus_tag="PLX85_01515"
     CDS             complement(987..2345)
                     /gene="mgtE"
                     /locus_tag="PLX85_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRC61886.1"
                     /translation="MQELEQAPAEASETFDALLADGRLEEAVAFARELHPADRAMLLA
                     TDDEDARRLLLSRLSPPDVGSALPYVERLYREDLLRELTPVEVADVLRSVPDDIATDV
                     IQELPEEVAEAALTALPLSLRRQIGLLLDYGTETAGGRMTGQRIAVGPDRTVREVIEF
                     LRSLRPDTDQPFYVYITDPANRLVGVLNLRWLITAAPETRVGSVMTDEVLSVRADADQ
                     EEAARLLKRYNLLALPVVDDAGRLLGTVTADDLLDVLEEEATEDMYRIVGVGTEEDLR
                     GVRTSVRNRLPWLGVNLLTALLAGFIVSLFEDTIARLAVIAAFMPMVAGMGGNAGIQT
                     VTVVVRSLALGRITPRDAWSVLRHELVVGLLIGLAIGLPVGVVGWAVNGSPALGLIVG
                     LAMCANITNGMVTGVFIPLLLHRLGQDPAISAGIWMTTFTDVIGFLLLLGLATLFISQ
                     LT"
     gene            2473..2967
                     /locus_tag="PLX85_01520"
     CDS             2473..2967
                     /locus_tag="PLX85_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRC61887.1"
                     /translation="MTSTAIDQRLYRDVIGRFATGVTVITFRTDHVTRGMTANAVASL
                     SLDPTLLIACIDKRTSAHAALAQADGFAVNILAEDQLEISRLFARPGLEGMGDVEYRT
                     AVTGAPILDGVLAWFDCAVHERFDGGDHTIFVGRVLELSLERPEADPLLFYAGGYRTL
                     GDSL"
     gene            3062..3138
                     /locus_tag="PLX85_01525"
     tRNA            3062..3138
                     /locus_tag="PLX85_01525"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3096..3098,aa:His,seq:gtg)
     gene            3223..4473
                     /locus_tag="PLX85_01530"
     CDS             3223..4473
                     /locus_tag="PLX85_01530"
                     /inference="COORDINATES: protein motif:HMM:NF036497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRC61888.1"
                     /translation="MYRWSLLLAAVAAAVLVVACGGGGGDDDATKISDRVLRLGEGVG
                     TEVEIFPGKVPDNLKDLLNPDKTSKDDPVELTPLPNAKLVGSARVTRVDGLHTFFLMY
                     EVKQDEPAVSEAARGLFDETPWQIVGGQSSDGVTAYRFQSTRSSDLVGTVVVQPLAST
                     ETFEVVISRGGKEKKLTLHRHAFTPVLGAELDEREGGVVVSRLGAGEGSNAGLQEGDR
                     VVKIGGKSIDNLASVSDALRQLGDGKDPVTSVMYVMQISPANPIVSPYVLPDPRPIPR
                     SFGNVAPYLVLDRTIPIAVQWSVEAQGSTYQFVLLSEATLTDVADNYRNVLKRQNLAI
                     TSDAAQGTGTTIEFSTPDNSMQGSVEVDSFVQDDHYTQISITVQAEPGFSNATRPQTT
                     PTPRATATATATAGAATATPTRTP"
     gene            4639..5052
                     /locus_tag="PLX85_01535"
     CDS             4639..5052
                     /locus_tag="PLX85_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61889.1"
                     /translation="MRVEKPCDFGGGLLRHACGREAISECVYCGHPFCERHGERGPDF
                     TDACARKRCSAKLRDVVAHQEWRERVRYANATSVCAHEECLERMHHQCSRCRLLFCSE
                     HVTQVTVVYNDVQPPKKDFALACFHCKDRRKLWNR"
     gene            complement(5075..5797)
                     /locus_tag="PLX85_01540"
     CDS             complement(5075..5797)
                     /locus_tag="PLX85_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRC61890.1"
                     /translation="MLRGRTVHLVRGGPLGRLEPTGETFPLSKVTLLAPIQPGKVVAI
                     GLNYRSHLEARVVPHDEPTHPEPFLKTPSSVIGPGQAIVLPADAGRVDEEGEVVVVIG
                     KRARNLRESEVDAHVLGYTAGNDVSAREWQRADLQWWRAKSSDTFTAVGPWIATDLDP
                     ERIPLTVRLNGEVVQQADTSQLIHSIRKCVAFVTSAMTLEPGDLVYTGTPGTTAELHA
                     GDTVEVEVGGVGVLTNPVAAAP"
BASE COUNT          928 a         1978 c         2050 g          851 t
ORIGIN      
        1 cgtctcgcgg aaccagcgga cggtggcggc aagccccgtc tcgaggtcag tctggggctg
       61 ccaaccccac acttcgcggg cgcgcgaggc atcgagcatg atgcgctgga tgtcgcccga
      121 ccgctccggg ccgtagatcg gggcgcgttc gtagccgctc aggcgcgcga ggtgttcgaa
      181 gatctgctgc gtgctcgtgc cgatgcccgt gccgatcagg caggtcgctg gctggtccgt
      241 cgcggcgcgc aaggccgcct cgacgacgtc ctctacatag acgtagtcgc gacgctggct
      301 gccatcgccg aagatcgtgg cctccgtgtt ggcgagcatg cgcagcgcga agatggccac
      361 gacacccgcc tcgccgtgtg ggtcctgcgc cgggccgtaa atgttgccgg gccggacgac
      421 ggacagctcg atgccgcccg ggcccgcgag tagccgcaga tactgctcgg cggcgaactt
      481 cgaggcgccg tagaccgatt ccggcgcggc cggcatttcc tcggtcacgg gcaccacggc
      541 gggctcgccg aagagcgcgc cgccggtgga gacgaagacg aagcgcttcg tccccgcctc
      601 gatggcggcg cgcatgaggt ggacgctgcc gagcacgttc gtctcgatgt cgacctcggg
      661 ctcgcgcatg gagaccgaga ccgaggcctg cgcggcgagg tggtacacga cctcaggacg
      721 cgcctcaagg accgcccgac gcaccgcgtc ggcgtcgcgg atgtcgacac ggaagtcgcg
      781 catctcagac ggccgaggcg tgcgcggcgg cacccagtcg aggccgctga cctcgcaccc
      841 ctcggcgacg aggcgggcga cgaggtgacc gccgacaaat ccggaagagc cagtaaccag
      901 tgcgggacca tgaagttggg acatgggccc atgttcgagt ccgcctcgga cgagagcaac
      961 cgagccggcg cgagcgcggg ggccggtcag gtgagctgcg agatgaagag cgtcgcgagt
     1021 ccgaggagca gcaggaaccc gatcacatcg gtgaaggtcg tcatccagat acccgcggag
     1081 atggccgggt cctggccgag gcggtgcagc aggagcggga tgaaaacgcc cgtcaccatg
     1141 ccgttggtga tgttcgcaca catcgccagg cccacgatga ggccgagcgc gggactgccg
     1201 ttcacggccc agcccaccac gccgaccggc agcccgatgg cgaggccaat cagcaagccg
     1261 acgaccagct cgtggcgcag cacggaccag gcgtcgcgcg gagtaatacg cccgagggcg
     1321 agcgaccgca cgacgacggt cacggtctgg atgcccgcgt tgccgcccat gcccgcgacc
     1381 atcggcatga aggccgcgat cacggcgaga cgcgcgatgg tgtcctcgaa cagggagacg
     1441 atgaagccgg cgagcagcgc ggtgagcagg ttcacgccga gccacggcag ccggttgcgg
     1501 accgaggtgc gcaccccgcg caggtcctcc tcggtgccca cgccgacgat gcggtacatg
     1561 tcctcggtcg cttcctcctc gaggacatcg aggaggtcgt ccgcggtgac ggtgccgagc
     1621 aggcggccgg cgtcgtcgac gaccgggagc gcgagcaggt tgtagcgctt gagcaggcgg
     1681 gcggcctcct cctggtcggc atcggcgcgg actgacagca cctcgtcggt catgacgctg
     1741 ccaacgcgcg tctcgggggc ggccgtaatc agccagcgga ggttcagcac gccgacgagg
     1801 cgattcgcgg ggtcggtgat gtagacgtag aacggctgat ccgtgtccgg gcgcagcgag
     1861 cgcaggaact cgatcacctc gcggaccgtg cggtccgggc cgaccgcgat gcgctggccc
     1921 gtcatgcggc cgcccgcggt ctccgtgccg taatcgagga gcaggccgat ctgccgccgc
     1981 agcgagagcg gcagcgccgt cagtgcggcc tccgcgacct cctcgggcag ctcctggatg
     2041 acgtccgtgg cgatgtcgtc cggcacggag cgcaggacgt cggccacctc gaccggcgtc
     2101 agctcgcgaa ggaggtcctc gcggtacagc cgctcgacgt acggcagcgc gctcccgaca
     2161 tcgggcggcg agagccggct gaggagcagc cggcgtgcgt cctcgtcgtc ggtggcgagc
     2221 agcatcgcgc ggtcggcggg gtgcagctca cgcgcaaagg cgacggcctc ctcgaggcgg
     2281 ccgtcggcga gcagcgcgtc aaacgtctcg gacgcctcgg cgggcgcctg ttccagttcc
     2341 tgcacgtccg aattgtcaca tgcccgcgct cacacccgga gcggcagcca tgacgaactg
     2401 cgcgtgttat ccgggtgtag gatgagattc agcccgcacg atcgtgcgga gtacgtcgag
     2461 gcgagggacg gcatgacgag cacggccatc gaccagcgcc tgtatcgcga cgtgatcgga
     2521 cgcttcgcca ccggcgtcac cgtgatcacg ttccggacgg atcacgtcac gcgcgggatg
     2581 accgcgaacg ccgttgcgtc gctatcgctg gacccgacgc tgctcatcgc gtgcatcgac
     2641 aagcggacct cggcgcacgc ggcgctggca caggcggacg ggttcgcggt gaacatcctg
     2701 gccgaggatc agctcgagat ctctcgcctg ttcgcgcgac cgggcctcga gggcatgggc
     2761 gatgtcgagt accgcacggc cgtgaccggg gcgcccatcc tcgatggggt gctggcatgg
     2821 ttcgactgcg ccgtgcacga gcgcttcgac ggtggtgacc acacgatctt cgtcggccgg
     2881 gtgctggagc tctcactcga gcgacccgag gccgacccgc tgctgttcta cgcgggcggc
     2941 taccgcacgc tcggcgactc gctgtagctc ctcgttgtgc atgggacggg gcgttggtgc
     3001 gcgcacgggc gaggatgcta cactcgccga gtgcggccgt gcgggccgca tgcttgtcgt
     3061 ggtgggtgtc gcctagcggt ctaaggcgcc aggttgtggc cctggagagc gagggttcaa
     3121 atcccttcac ccaccccaca tcctccctcg accgtccgca tacgccgcca gcgaccgccc
     3181 tgccgaaccc gactcgctcc cacgagcctc gaggggtccg ccatgtaccg atggtccttg
     3241 ctcctggcag ccgtggctgc cgccgtgctc gtggttgcct gcggcggcgg agggggtgac
     3301 gacgacgcga cgaagatctc ggaccgcgtg ctgcgcctcg gcgagggcgt cggcacggag
     3361 gtcgaaatct tcccggggaa ggtccccgac aacctcaagg acttgctgaa cccggacaag
     3421 acctcgaagg acgacccggt cgagctgacg ccgctcccga acgcgaagct ggtgggatcg
     3481 gcgcgcgtga cccgcgttga cggcctgcac acgttctttc tgatgtacga ggtgaagcag
     3541 gacgagcccg cggtctccga ggccgcgcgc ggcctgttcg atgagacgcc ctggcagatc
     3601 gtgggtggcc agtcgagcga tggcgtgacg gcgtatcgct tccagagcac gcgttcgagc
     3661 gacctggtgg ggacggtcgt cgtgcagccg ctcgccagca ccgagacgtt cgaggtcgtg
     3721 atctcgcggg gcgggaaaga gaagaagctg accctgcacc ggcacgcctt cacgccggtg
     3781 ctgggggcgg agctcgacga gcgcgagggc ggggtcgtgg tgtcccgcct cggcgcgggc
     3841 gagggctcga acgccggcct gcaggaaggc gaccgcgtcg tcaagatcgg tggcaagtcg
     3901 atcgacaacc tggcctccgt gagcgacgcg ttgcgccagc tcggcgacgg caaggacccc
     3961 gtgacgagcg tgatgtacgt gatgcagatc tcgccggcga acccgatcgt gtcgccctac
     4021 gtgctgcccg acccgcgtcc catcccgcgc tccttcggga acgtcgcgcc gtacctcgtc
     4081 ctcgaccgca ccatcccgat cgcggtgcag tggtccgtgg aagcccaggg gtcgacgtac
     4141 cagttcgtgc tgctgagcga ggccacgctg acggatgtcg cggacaacta ccgcaacgtg
     4201 ctgaagcggc agaacctcgc catcacttcg gacgccgcgc agggcacggg cacgacgatc
     4261 gagttcagca cgccagacaa ctccatgcag gggtcagtcg aggtggattc gttcgtccag
     4321 gacgaccact acacgcaaat ctcgatcacg gtccaggccg agcccggctt ctcgaacgcg
     4381 acgcgtccgc agaccacgcc gacgccgagg gccacagcga ccgcgacggc caccgccggc
     4441 gccgccaccg ctactccgac ccggacgccg taacgctcca tccgtggatg cccgaaacgc
     4501 ctcgggcatc cacggccagc gcctcgacgc accgcgtggc cgatcgcgtc cgtgtcggcg
     4561 gacgatcagg ggcccctgcc gccgtgcgac aatgggagtc tctggggtcg ctttcgctca
     4621 cgagtaaggg atgcctgatt gcgcgtcgag aagccgtgcg acttcggcgg cgggctcctg
     4681 cgtcacgcct gcgggcgcga ggctatctcg gagtgcgtgt actgcggcca tccgttctgc
     4741 gagcggcacg gcgagcgtgg ccccgacttc accgatgcct gtgcacgcaa gcgttgctcg
     4801 gcgaagctgc gagacgtggt cgcccaccag gaatggcgtg agcgcgtgcg ctacgcgaat
     4861 gcgacgagcg tgtgcgcgca cgaggaatgc ctcgagcgaa tgcaccatca gtgctcacgc
     4921 tgccggctcc tcttctgctc ggagcacgtg acgcaggtca ccgtcgtcta caacgacgtg
     4981 cagccgccga agaaagactt cgcactcgcc tgtttccact gcaaggaccg gcggaagctc
     5041 tggaaccgat agcgcgcgca gcacgctcga tccgtcaggg cgcggctgcc accggattcg
     5101 tgagcacgcc gacgccgccg acttccacct cgaccgtgtc gcccgcgtgc agctcagcgg
     5161 tcgtgcccgg cgtcccggtg tagaccaggt cacccggttc aagcgtcatc gcgctcgtga
     5221 cgaacgccac gcactttcgg atcgagtgaa tgagctgtga ggtgtcggcc tgctgcacga
     5281 cctcgccgtt cagccgcacg gtcagcggga tacgctcggg gtcgaggtcc gtggcgatcc
     5341 acggcccgac ggccgtgaac gtgtccgagg acttcgcccg ccaccactgc agatcggcgc
     5401 gctgccactc gcgcgcggag acatcgttgc cggcggtgta gccgaggacg tgcgcatcta
     5461 cctcggactc tcgcaggttg cgcgcccgct tcccgatcac caccaccacc tcgccctctt
     5521 catcgacgcg gccggcgtcg gcagggagga cgatcgcctg gccgggcccg atgaccgagc
     5581 tcggcgtctt caggaacggc tcggggtgcg tgggctcgtc gtgcggcacg acccgcgcct
     5641 cgaggtggct gcgatagttg aggccgatcg cgacgacctt gcccggctgg atgggcgcga
     5701 gcaacgtcac tttcgagagc gggaacgtct cgcccgtcgg ctcgaggcgg ccgagcgggc
     5761 caccgcgcac gagatgcacc gtccgtccgc ggagcaccgc ggtgctc
//
LOCUS       DAORPG010000036         5739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_782_length_5739_cov_3.948804, whole genome shotgun sequence.
ACCESSION   DAORPG010000036 DAORPG010000000
VERSION     DAORPG010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_782_length_5739_cov_3.948804"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PLX85_01545"
     CDS             <1..629
                     /locus_tag="PLX85_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRC61891.1"
                     /translation="RTFMDAEMRRRRADPADDLLTRLEVARATGELSDSEAFLYSLIL
                     LVAGNETTTNLLGMLLMHLARDPELFEELKADRTLVPRAVEETARWGSPVQWVTRVAT
                     APYEVGGTVIPAGGKVVLFYASANRDPRKFSDPDRLDIHRDTTGHLAFGHGLHFCLGA
                     HLARLETVTAVNHLLDEVDGLELAGPVRWGTTPSLQGPASLPVRIRRQ"
     gene            689..1099
                     /locus_tag="PLX85_01550"
     CDS             689..1099
                     /locus_tag="PLX85_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRC61892.1"
                     /translation="MPSIPTAGSIAIADLTGDAVVRVPAGATVAEVAKVIADKQVGAV
                     VVGDDARPTALVTERDVVKVVASGKDPAATRAADVASTRLLWCEADETVDQAAIRMTD
                     HSIRHLLVERDGGLVGIVSARDLLGVYATDADQV"
     gene            complement(1090..5718)
                     /locus_tag="PLX85_01555"
     CDS             complement(1090..5718)
                     /locus_tag="PLX85_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRC61893.1"
                     /translation="MGELGEDGRLSYLLYFDGDDFTSAYQELDARYYAGEGAAFAELG
                     RAQSAFVAAMDSLDVEAARQLCEPEFRWSSPTGALAEPLRTIDEVISWWRDRAGQVDS
                     LRNWTSAIAWVSPDVAVSIGEARGISRDGADYAWSGIFVATFRDGLFDSVYGFEPEDE
                     EVAFEYAESQAEQRRSRLAVANASSRALGEAFAALQADNPSAVASLFSAEVVYEDRRP
                     LAGALETGVDYLNEVVPALLSQYDNFETHILAVRGDRLCLAWSRWSDESGNEATNLHL
                     TELGEDGLITRLMYFVGDDFWSAYRELERRYYAGEGAPYAVGGRAAADWVIAISNGDI
                     EGVRRASHPDFRWYATPSALKDSERTVDDMFRWWQERGRQVSSQRHWVPALVWLSPNC
                     AVSRGEIAAVGPDGEQYDWNLIIVTECRDGLVLAAREFDNEDSAFAHAESVVTPKPSR
                     LSMMNAPSRMAVRAVAALRAGDLDSLVEAYADDYIHADHRRLSGEPITDRASIRNAWE
                     RIIAPYNRFEIDVIAVRGERLHLARSRWSDESGNQSIGLVVSETNESGQIAFQDRFDE
                     DDFAAAYAELERRYYAGEGAPYAEGGAVLTHMVLAENRGDLDGAFKAYLRPGLRIENR
                     SRSLFPSRTAEELRRGVEELGDMVTSYRVWSPVMCWLSNRWVVARHEREADGKKFSWS
                     RLYVGEISDGLFTTLCEFDVDDEDAAFAYAEERIRAVPSRLAVRNRAGDAGEAMMAAM
                     RAHDANRVVEPYAGEFVYDDRRRLSGEPISSVDAMRTAAERVFRQYTRFENRMLAVRG
                     DRLALGWSLWSDEAGNEATHLHLVGVDEDGLINYHARFEADDFDSAYRELESRYYAGE
                     GADFAGCGLVLAAFLRAADALDVEAARLMCAPGFAVFAPPTALTAQERSLDEFFQWQR
                     ERSGQVSSVKYALSAAHWLSPACGVFLADVQARGTDTENYGWARIYVAEFSGGLLASI
                     RQFEDEGAAFAYAEERIRVPASRLVVSNRASDVGLLILAGMRAHDLEGTVKHWADEFV
                     YDDRRRYSGDVLTDRTGMRSAMQRIFRQYSRFESRVLAVRGSYLNLASHCWSDESGNQ
                     SSSLILAEIDGDDHIVYEARFDEDDFNGAFEELERRYYSGEGAAFAEYGNAASQLMTA
                     ENSGDYDKLFNELAAPGLRVESRAGTIFPERTPAELRDSVELFHAMVASARTWYSVVR
                     WLSPSCAVVRQEREAIGEEGEHYEWSRIYVAEFERGLYTGICDFDVEFEAQAFAYAEE
                     RLRAAQSPLLLSNRASEAGTALTAALAAADVDAALALYSDQFLYADRRRIGGALVHDF
                     SSVQKSIEAFCLVYSQAEEVTLAVRGNKFALGSVRIADDRGNFIHNLEVLEVGEDDRI
                     VFHATYDLDDFDSAYRELETRYYAGEGAASAECGLFAAEYVATMNRGDFDTMFNELSS
                     PGFRFENRGRSPFPDRSARELRASFEDLYARLASVRSWFSSLYWVAPTTSIGRLEREG
                     LGHDGEHYAWTRILVNQYREGRLASTCEFDADDDAAALAYAEELAAQT"
BASE COUNT         1030 a         1896 c         1811 g         1002 t
ORIGIN      
        1 tgcgcacgtt catggacgcc gaaatgcgac ggcgccgggc tgacccggcc gacgatctgt
       61 tgacgcgcct ggaggttgcc cgggcaaccg gcgaactgtc cgacagcgag gcgttcctct
      121 actcgctgat cctgctggta gccggaaacg agacgaccac aaacctgctc ggcatgctgc
      181 tgatgcacct ggcccgggac cccgaactct tcgaggagct caaggccgac cgcaccctgg
      241 tgccgcgcgc ggtggaggag accgcgaggt ggggctcgcc ggtgcagtgg gtcacccggg
      301 tggcgaccgc gccctacgag gtcggcggga cagtgatccc cgcgggcggc aaggtggtgc
      361 tgttctacgc atcggccaat cgcgatccgc gcaagttctc cgacccggac cggttggaca
      421 tccaccgcga caccaccggc cacctggcgt tcggccacgg cctacatttc tgcctcggcg
      481 cacacctggc tcggctggag acggtaaccg ccgtcaacca cctgctcgac gaggtcgacg
      541 gcctggaact ggccggaccg gtgcgatggg ggacgacgcc gtcgctgcag ggacccgcgt
      601 cgttgccggt tcggatccgg cggcagtgag gtcagcctga cttcaggcag gtcagggcgg
      661 ggatgagagc atgtccggca tgactgagat gccctccatc ccgacggccg gctcgatcgc
      721 catcgccgac ctcaccggcg acgcggtagt tcgcgtgccc gccggcgcca ccgtcgccga
      781 ggtcgccaag gtgatcgccg acaagcaggt gggcgcggtc gtggtcggtg acgacgcccg
      841 gcccaccgcg ctggtgaccg agcgtgacgt ggtcaaggtg gtggcgtccg gcaaggatcc
      901 cgccgcgacg cgggccgccg acgtggccag cacgcgcctg ctgtggtgtg aggccgacga
      961 gacggtcgac caggcggcga tccggatgac tgaccattcg atccggcacc tgctggtcga
     1021 gcgggatggg ggattggtgg gcatcgtgtc cgcgcgcgac ctgctcgggg tgtacgccac
     1081 cgacgccgat caggtctgag ccgcgagttc ctcggcgtag gcgagggcgg cggcgtcgtc
     1141 gtcggcgtcg aattcgcatg tcgatgcgag ccggccttcg cggtattggt tgacgagaat
     1201 ccgcgtccag gcgtagtgct cgccgtcgtg cccaagcccc tcgcgttcca ggcggccaat
     1261 agaggtggtc ggcgccaccc agtagagact cgagaaccac gaccgcaccg aggcgagccg
     1321 tgcgtagaga tcctcgaaac tggcccgaag ttcgcgtgcc gaacgatccg ggaaggggga
     1381 tcgcccccgg ttctcaaagc ggaagccggg gctgctcagc tcgttgaaca tggtgtcgaa
     1441 gtcgccgcgg ttcatcgttg ccacgtactc ggcggcgaat agaccgcact ccgcagacgc
     1501 cgcgccttcg cccgcataat agcgggtttc gagttcacgg taggcagagt cgaagtcatc
     1561 taggtcgtag gtcgcatgaa aaacgatgcg gtcatcttct cccacctcga gaacctccag
     1621 attgtggatg aagttgccgc ggtcatcagc gatacgaacc gaacccagtg cgaacttgtt
     1681 gccgcggacc gctagcgtga cctcctctgc ctgggagtac accaagcaga aggcctcgat
     1741 gctcttctgc accgaggaaa agtcatggac cagcgcccca ccgatccgcc gacgatcggc
     1801 atagaggaac tgatcggagt aaagggctaa cgctgcatcg acgtccgccg ccgccagcgc
     1861 ggcggtgagt gccgttcccg cttcactggc ccgattactc agcaggagcg ggctttgcgc
     1921 ggcacgcagc cgctcttcgg catacgcgaa ggcctgcgcc tcgaactcga cgtcgaaatc
     1981 acagatcccg gtgtagaggc cgcgctcgaa ctcggcgacg tagatacggc tccactcata
     2041 gtgctcgccc tcctcgccga ttgcttcgcg ttcctgacgc acgacggcac agctggggga
     2101 caaccagcgg acaaccgaat accaggtgcg tgcggaggcc accatcgcgt ggaaaagctc
     2161 gacgctgtcg cgcagttccg ccggcgttcg ctcaggaaaa atcgtgcccg cgcggctctc
     2221 gacccgcagt cccggagctg ccagctcatt gaagagcttg tcgtagtcgc cgctgttttc
     2281 ggctgtcatc agctggctcg ccgcgttgcc atactccgcg aaggctgcac cctctccgga
     2341 gtagtagcgg cgttcgagtt cttcgaaggc gccgttgaag tcgtcctcgt cgaagcgggc
     2401 ctcgtagaca atgtggtcgt cgccatcgat ctcagccaag atgaggcttg acgattggtt
     2461 tccgctctcg tctgaccaac aatgacttgc caggttgaga taagaaccgc ggactgcgag
     2521 cacccgactc tcgaaccggc tgtattgcct gaatatccgc tgcatcgccg agcgcatgcc
     2581 ggtgcgatcg gtcagcacgt cgccgctata cctccgtcga tcgtcgtata cgaactcgtc
     2641 tgcccagtgc ttaacagttc cctcgaggtc gtgcgcgcgc atacccgcaa ggatgaggag
     2701 gcccacatcg ctcgcacggt tgctaaccac aagcctgctg gcaggaacgc ggatccgctc
     2761 ctcggcgtag gcgaaggccg cgccctcgtc ttcgaactga cggatggatg ccagcagacc
     2821 accgctgaac tcagcgacat atattcgtgc ccaaccgtag ttctcggtgt cggtcccgcg
     2881 tgcctgaacg tcagccagga agacgccgca tgcgggcgac agccagtggg ccgctgagag
     2941 ggcgtacttg accgacgaga cttgaccgga acgttcgcgc tgccattgga agaactcatc
     3001 gagtgatcgc tcctgcgcgg tcaacgcggt gggcggggca aataccgcga agcccggcgc
     3061 gcacatcaat cgagcggctt cgacgtcgag ggcatccgct gctctgagaa acgcggcgag
     3121 gaccaagccg caccctgcga agtcggcgcc ctcgccggcg tagtagcggc tttccagttc
     3181 ccgataggcg ctgtcgaaat cgtcggcttc gaagcgggcg tgatagttga tcaggccgtc
     3241 ctcgtccacg ccgaccagat gcaggtgcgt ggcctcgttc ccggcctcat ccgaccagag
     3301 gctccagccg agagccaggc gatcaccgcg caccgcgagc atgcgattct cgaaccgcgt
     3361 gtactgccgg aagactcgct cagcggcggt gcgcatcgca tcgacgctgg agatgggctc
     3421 gccactgagt cggcgccggt catcatagac gaattcgcca gcgtatggtt cgacgactcg
     3481 gttggcatcg tgcgctcgca tcgcggccat catggcctca cccgcatcac cggcacggtt
     3541 gcgtaccgcc agtcggctgg ggaccgcgcg gatccgctcc tcggcgtagg cgaaggccgc
     3601 gtcctcgtcg tcgacatcga attcgcacaa cgttgtgaac aacccgtcgc tgatctcgcc
     3661 gacatatagc ctcgaccagg aaaacttctt cccgtcggcc tcgcgttcat gtcgcgccac
     3721 cacccagcgg ttggatagcc agcacatcac cggactccac acccggtatg acgtcaccat
     3781 gtcgccgagt tcctctacgc cccggcgaag ttcctcggcg gtgcggctcg gaaaaagtga
     3841 acgcgagcga ttctcgatcc gaagacccgg acggaggtag gccttgaacg cgccatccag
     3901 atcaccgcga ttctctgcta ggaccatgtg ggtcagcacg gcgccccctt ccgcgtaggg
     3961 tgcgccctct ccggcgtaat agcgtcgttc gagttcggcg taggcggcgg cgaagtcgtc
     4021 ctcgtcgaag cggtcctgga atgcgatctg cccactctcg ttggtttccg agacgacaag
     4081 tccgatggac tgattcccgg attcatccga ccatcggctg cgcgcaagat gcagtcgctc
     4141 gccccgaacc gcaatgacgt cgatctcgaa ccggttgtag ggagcaataa ttcgctccca
     4201 tgcgtttcgg atgctggcgc gatcagtgat cggttcgccg ctgagccggc ggtggtcggc
     4261 gtgaatgtag tcgtccgcgt aagcctcgac gaggctgtcg aggtcacccg cgcggagtgc
     4321 ggcgacggcc cgcaccgcca tcctggacgg ggcgttcatc atcgagagtc gagacggctt
     4381 cggcgtgacg accgattcgg catgggcgaa cgccgagtcc tcattgtcga actcccgcgc
     4441 tgccagcacg agtccatcgc ggcactcggt cacgatgatc aagttccagt cgtactgctc
     4501 tccgtctgga ccgacggccg caatctcacc gcggctgaca gcgcagttcg gcgatagcca
     4561 caccaacgcg ggcacccagt ggcgctggga cgatacttgg cgtcctcgct cctgccacca
     4621 tcggaacatg tcgtcgactg tgcgttctga gtctttgagc gccgacggcg tcgcgtacca
     4681 acggaagtcg gggtgggaag cgcggcgaac gccctcgatg tcaccgttgc tgatcgcaat
     4741 cacccagtcg gcggccgccc gtccaccgac cgcgtacggt gcgccctcac cggcgtagta
     4801 gcggcgctcg agttcgcgat aggcgctcca gaaatcgtcc cccacgaagt acatcaggcg
     4861 cgtgatgagc ccatcctcgc cgagttcggt cagatgcaga ttggttgcct cgttcccgga
     4921 ctcgtccgac caccgactcc acgcgaggca tagccggtcg ccacggacag cgagaatgtg
     4981 ggtctcgaag ttgtcgtatt ggctcagtag tgccggaaca acctcattca agtaatcgac
     5041 gcccgtttcc agcgcaccgg cgagtggacg cctgtcttcg taaacgacct cggctgaaaa
     5101 tagactggcg acggcgctgg gattgtcggc ctgcaacgca gcaaatgcct cgcctagcgc
     5161 ccggcttgac gcgttcgcta ccgcaagccg actgcgtcgc tgttctgcct gcgattccgc
     5221 gtactcgaag gcgacttcct cgtcttcggg ttcgaatccg tacaccgaat cgaagagccc
     5281 atcgcggaag gtggcgacga agattcctga ccaggcatag tcagcaccgt cgcggctgat
     5341 cccacgcgcc tcgccgatgg agactgctac gtctggcgac acccacgcga tcgccgacgt
     5401 ccagttccgc agcgagtcaa cctgtccggc acggtcccgc caccatgaga tcacctcatc
     5461 gatcgtgcgc aacggctcag ccaacgcccc ggtcggcgac gaccaacgga attcgggctc
     5521 acacagctgc cgggcagcct cgacatccaa gctgtccatt gccgcgacga aggcggactg
     5581 cgcgcggccc agttctgcga aggccgcacc ctcgccggca tagtaacgcg cgtccaattc
     5641 ttggtatgca gaggtgaagt cgtccccgtc gaagtacagc agatagctga gtcgcccgtc
     5701 ctcaccgagt tcgcccacgt gaagattggc cgccgtgtt
//
LOCUS       DAORPG010000037         5715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_789_length_5715_cov_3.090283, whole genome shotgun sequence.
ACCESSION   DAORPG010000037 DAORPG010000000
VERSION     DAORPG010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_789_length_5715_cov_3.090283"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..741
                     /locus_tag="PLX85_01560"
     CDS             121..741
                     /locus_tag="PLX85_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRC61894.1"
                     /translation="MSTRRSYQSRLRAERARETRLAIRRSARELFEEQGFTATTVAQI
                     AARAGVAVPTVYAAFESKSGIVREMMDELEELAGGEDAGERVLAEEDPERQLRTFVHF
                     VRTLFERGAPVLRAAMQVRDDEDVQRMAAVGNGRRLEGCRLLIARWGELGALATDVDA
                     GRAAEGMWLLTSAEQYFLGTDTLGWSPDEYETWLAETLSRLLLRRG"
     gene            complement(753..1868)
                     /locus_tag="PLX85_01565"
     CDS             complement(753..1868)
                     /locus_tag="PLX85_01565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC61895.1"
                     /translation="MLDEPALRTLGLRFAERDAKDLDAYPVANVDDLKAAALLGAPFA
                     PSLGGHGWTAAEAARAIELLASYSPSTALIWAMPLGFAGIYSMDVEVVPPEARTAWSA
                     QVDEVTARYRAGQLYAACNSERGAGGSVAATQTTARRGADGVFRLTGDKILATSGSYA
                     DVFFSTAKVTPQDLPGAGVVEFFLVPTRTPGVEVLTDWDGFGMRSTESHTVRYQDAPA
                     EGILGFPDFLARIQPLEYWFNLFAAIPLGCARGMLRALSTSTPAAPALRVRLNDARMR
                     YEALRAYLLETAAAWRPAGGPEYAARVLRTKTYVGQEATALCATLFALGGGRHYRRND
                     PLARLLADSFAGTALRPPLPLALDAMLDQFGSSEEYA"
     gene            1941..2447
                     /locus_tag="PLX85_01570"
     CDS             1941..2447
                     /locus_tag="PLX85_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRC61896.1"
                     /translation="MGVVERHFTVSGFVSQGGCTALHWHRLGLWLPPGGHIEENEDPI
                     QAVLREVHEETGMRAEIVPTSGRYGYAQPRQLPPPVTIGVYDIEEPGRPHQHIDLIYF
                     TRPRAGESLELPSDGNGWLWVSEDELRTESELERPDGAGSAVIPEDVRQLGLDAIAAV
                     RALEADAA"
     gene            2447..3772
                     /gene="serS"
                     /locus_tag="PLX85_01575"
     CDS             2447..3772
                     /gene="serS"
                     /locus_tag="PLX85_01575"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRC61897.1"
                     /translation="MLSIQTIREQTDAVRQACTDRQMDVPIDEILRADSEHRTLLGEV
                     EALRAERNRASKAIGAAKDAAERQQMIEAQRAAGSELDALEGRLREADATLTSLLLQV
                     PNLFDERVPLGGESDAVVVIEGDGRAGTEQAVEPTRRAADDPAPPEDASRRPHWEIGE
                     ALGLIDFERGAKVAGSRFYILKGDAARLQRALIAWMIDVHREQGYREVYVPFVVKEEM
                     LVGTGQLPKFAETMYHDVEDDLWLVPTAEVPVTNMYRDEIVAASALPIRHVAYTPSFR
                     RERMSAGRDVRGIKRGHQFDKVEMVQFTEEGASWDALEQLVQDALAIVRALGFRYRVI
                     RLASADITFASAMTYDIEVWAPGSREWLEVSSVSNFLDFQARRANLRYRDAEGRVRHL
                     NTLNGSGLALPRVLATVLEQYERPEGIEVPAPLRPYLGGLELIEAQPAF"
     gene            complement(3783..5045)
                     /gene="murA"
                     /locus_tag="PLX85_01580"
     CDS             complement(3783..5045)
                     /gene="murA"
                     /locus_tag="PLX85_01580"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRC61898.1"
                     /translation="MTTKTATRLVIRGGRPLRGRVTVSGSKNASLYALAAGLLTAEPV
                     TLRRVPAIADIGEMAEVLRSLGADVTIEDETVHIEAREVNGRVPPADQVAALRASFLV
                     MGPLLARFGEAACPPPGGDVIGVRPLDVHLAGFNALGADVRREGQEFVARATRLRGTR
                     IFLDYPSVLGTVNVLFAATLAEGTTTIINAAAEPEVAMAVEMLNLMGAKIAGGGTNVI
                     TVDGVEVLHGVDFTVIPDRIEAGTYLLAGVATGGDVEVIGATPDHLDSLITKLREAQV
                     LVEPCEGGLRAARGDRFQAVQLQAVPYPGFATDLHAPMAAALTQASGVSIIHERVFEN
                     RLLYVGELRSMGARITTGGQAVMIEGPTPLFGTSVRALDVRAGAAVVLAGLCAEGETT
                     IRDITHIDRGYAHLVERLTALGADVSRE"
     gene            5129..5380
                     /locus_tag="PLX85_01585"
     CDS             5129..5380
                     /locus_tag="PLX85_01585"
                     /inference="COORDINATES: protein motif:HMM:NF021452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="HRC61899.1"
                     /translation="MSKVVLGKEDREDAVRRLRAYFERERGEELGELGATLLFEFLAE
                     EIAPHFYNRGLADAQSLVARFTDSLDADLEAAKVFPPRA"
     gene            complement(5396..>5715)
                     /locus_tag="PLX85_01590"
     CDS             complement(5396..>5715)
                     /locus_tag="PLX85_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRC61900.1"
                     /translation="KIVYSTTLEAPLTARTRTERTFDAEAVRRLKAESSADLAIGGPE
                     LAAHAFRAGLIDECHLVLHPVAVGAGKPALPRDFRLDLELLHERRFNCGVVDLHYRVR
                     RPA"
BASE COUNT          918 a         1691 c         2296 g          810 t
ORIGIN      
        1 gccacgatgc cgagtacaaa cacgaccgga ccgacggccg cgatgaagcc aaggattctc
       61 accattttat atctcctcca tatattcatt agagtttcat atacttgtta agctggcgac
      121 gtgtcaacgc gtcgctccta ccagtcacgg ctccgggcag aacgtgcccg agagacccgt
      181 ctcgccattc ggcgcagcgc ccgagagctg ttcgaggaac aggggtttac ggcgacgacg
      241 gtcgctcaga tcgctgcgcg tgcgggcgtc gcggtcccca ccgtgtacgc ggccttcgag
      301 tcaaaaagcg ggatcgtccg ggagatgatg gacgagctgg aggagctggc cgggggcgag
      361 gatgccggcg agcgtgtgct ggccgaagag gatccggaac gccagctacg cacattcgtg
      421 cacttcgtac gcacgctgtt cgagcgcggc gcgcccgtcc tgcgggcggc gatgcaggtg
      481 cgggacgacg aggacgtgca acgcatggcg gcggtaggga acggacgccg gctcgagggg
      541 tgccgcctcc tcatcgcgcg ctggggggag ctgggcgcgc ttgcgacgga tgtcgacgcc
      601 ggacgggcgg ccgagggaat gtggctgctg acgagcgcgg agcagtattt cctcggcacg
      661 gacacactcg ggtggtcgcc agacgaatac gaaacctggt tagcggagac gctgtcgcgg
      721 ttgctgctgc ggcgaggctg acgccgtcgg cctcaggcgt attcctcgga ggagccgaac
      781 tggtcaagca tggcgtcgag ggcgaggggg agcgggggac gaagggcggt gcccgcgaag
      841 gagtcggcga gcaggcgggc gagggggtcg ttgcggcggt agtgacggcc gccgccgagg
      901 gcgaaaaggg tggcacagag cgcggtcgct tcctggccca cgtaggtctt ggtgcggagg
      961 acgcgggcgg catactcggg gccgccggct ggacgccagg cggcggcggt ctcaaggagg
     1021 taggcgcgca aggcctcgta acgcatgcgg gcgtcgttca gccggacgcg gagcgcgggg
     1081 gcagcgggcg tagacgtcga aagggcgcgc agcatgcctc gggcacagcc gagggggatg
     1141 gccgcgaaga gattgaacca gtactcgagg ggttggatgc gcgcgaggaa gtcgggaaag
     1201 ccgaggatgc cctcggcggg agcgtcctgg tagcggaccg tgtggctctc ggtcgaccgc
     1261 atgccgaagc cgtcccagtc ggtgagcacc tcgacgcccg gagtgcgcgt cgggacgagg
     1321 aagaactcga cgacgcccgc gccgggaagg tcctgcggcg tgacctttgc cgtggagaag
     1381 aagacatcgg cataggagcc ggaggtggcg aggatcttgt cgccggtgag gcggaagacg
     1441 ccgtccgcgc ctcggcgggc cgtggtctgg gtggcggcga ccgaaccgcc cgcgccccgc
     1501 tcggagttac aggcggcgta gagctggccg gcgcggtatc gcgcggtgac ctcgtcgacc
     1561 tgagccgacc aggcggtgcg cgcctcggga ggcacgactt cgacgtccat ggagtagata
     1621 ccggcgaagc cgaggggcat ggcccagatg agggcggtcg agggggaata ggaggcgagc
     1681 agctcgatgg cgcgcgccgc ctccgccgcc gtccagccgt gtccgccgag ggaaggcgcg
     1741 aagggtgcgc cgaggagggc ggcggctttc aggtcgtcga cgttggcgac cggataggcg
     1801 tcgaggtcct tggcgtcccg ctcggcgaag cggaggccca gggtgcgcaa tgcgggttcg
     1861 tcgagcatgg caggctcccc tcggatgagt gatcttagcg tttcgttgcc gcctcgatgg
     1921 ggcgctgcta cgctcggccg atgggcgtgg tggagcggca cttcacggtc agcgggttcg
     1981 tgagccaggg tgggtgcacg gcgctgcact ggcaccgcct cgggctgtgg ctgcccccgg
     2041 gaggccacat cgaggagaac gaggacccga tccaggcggt gctgcgcgag gtgcacgagg
     2101 agacggggat gcgcgcggag atcgtgccaa cctcggggcg ctatgggtat gcgcaacccc
     2161 ggcaactgcc gccgccggtg accatcggcg tgtacgacat cgaggagccc gggaggcccc
     2221 atcagcatat cgacctgatc tacttcacgc gtccacgagc cggggagtcg ctcgaactcc
     2281 cctcggacgg caacggatgg ctgtgggtgt ccgaggacga gctgcggacg gagtccgaac
     2341 tcgaacggcc ggacggcgct gggtccgcgg tcatcccgga ggacgtgcgt cagcttggcc
     2401 tcgacgcgat cgcggcggtg cgggcgctgg aagcggacgc tgcctagtgc tctcgataca
     2461 gacgatccgc gagcagaccg acgcggtgcg acaggcgtgc acggaccggc agatggacgt
     2521 gccgatcgac gagatcctgc gcgcggacag cgagcaccgg acactcctcg gcgaagtgga
     2581 ggcgctgcgg gccgagcgca accgcgcgag caaagcgatc ggagcagcga aggacgcggc
     2641 agagcgccag caaatgatcg aggcgcaacg ggcggccggg agcgagctcg acgccctcga
     2701 ggggcggctg cgggaggcgg acgcgacgct cacctcgctg ctgctgcagg tgcccaacct
     2761 cttcgacgag cgagtgccgc tcggcggcga gtcggacgcg gtcgttgtga tcgaagggga
     2821 cgggcgcgcg ggcacagaac aggccgtcga gccgactcgg cgcgcggcgg acgacccggc
     2881 gccccccgag gacgcctcac gacgtccgca ctgggagatc ggcgaggccc tggggctgat
     2941 cgacttcgag cgcggggcga aagtcgcggg ctcgcgcttc tacatcctca agggcgacgc
     3001 cgcgcggctc cagcgcgcgt tgattgcctg gatgatcgac gtgcatcgcg agcaggggta
     3061 ccgcgaggtg tacgtcccct tcgtggtgaa ggaggagatg ctcgtcggca ccgggcagct
     3121 gccgaagttc gccgagacga tgtaccacga cgtcgaggac gacctgtggc tcgtgccgac
     3181 ggccgaggtc ccggtgacga acatgtaccg cgacgagatc gtggcagcct cggcgctgcc
     3241 gatccggcac gtggcgtaca cgccctcgtt ccggcgggag cggatgagcg cgggccgcga
     3301 cgtgcgcggc atcaagcgcg gccaccagtt cgacaaggtc gagatggtgc agttcaccga
     3361 agaaggcgcg tcgtgggacg ccctcgagca gctggtgcag gacgcgctcg cgatcgtgcg
     3421 ggcgctgggg ttccggtacc gggtgatccg gctggcctcg gcggacatca cgttcgcctc
     3481 ggcgatgacc tatgacatcg aggtgtgggc gcccgggtcg cgggagtggc tcgaagtgtc
     3541 gtcggtgtcg aacttcctcg acttccaggc gcggcgggcg aacctgcgct accgggacgc
     3601 cgaggggcgg gtgcggcacc tcaacacgct gaacggctcc gggctggcat tgccgcgcgt
     3661 cctggcgacc gtgctggagc agtacgaacg gccggagggc atcgaggtgc ctgcgccgtt
     3721 gcggccgtac ctcggcgggc tcgagctgat cgaggcgcag cccgcgttct agaccgaccg
     3781 acctattccc tcgagacgtc ggcgccgagg gcggtgaggc gctcaacgag gtgggcgtag
     3841 ccgcggtcaa tgtgggtgat gtcgcggatg gtcgtctcgc cctcggcgca gaggccggcg
     3901 aggacgacgg cggcgccggc gcggacatcg agggcccgga ccgaggtgcc gaagagcggc
     3961 gttgggcctt cgatcatgac ggcctggcca cctgtcgtga tgcgcgcgcc catggagcga
     4021 agctcgccga cgtagagcag gcggttctcg aaaacacgct catggatgat cgagacgccg
     4081 ctagcctgcg tgagggcagc ggccattggc gcgtggaggt ccgtggcgaa gcccgggtag
     4141 gggacggcct ggagctgcac ggcctggaag cggtcgccac gcgcggcgcg caggccgcct
     4201 tcgcagggtt cgacgagcac ctgggcctct cggagcttgg tgatgaggct gtcgaggtgg
     4261 tccggcgtcg cgccgatgac ctcgacgtca ccgccggtgg cgacgcccgc gagcaagtag
     4321 gtgcccgcct cgatgcggtc ggggatgacg gtgaagtcga cgccgtggag cacttcgacg
     4381 ccgtcgacgg tgatgacgtt cgtgccgccg ccagcgatct tcgcgcccat caggttgagc
     4441 atctcgacgg ccatggcgac ctcgggctca gcggcggcat tgatgatggt ggtggtgccc
     4501 tcggcgaggg tggcggcgaa gaggacgttc accgtgccga ggacgctggg gtagtcgagg
     4561 aagatgcggg tgccgcggag gcgggtagcg cgagcgacga actcctggcc ttcgcggcgg
     4621 acgtcggcgc cgagggcgtt gaagccggcg agatggacat cgagggggcg gacgccgatg
     4681 acgtcgccgc cggggggcgg gcaggcggcc tcgccgaagc gggcgagcag tgggcccatg
     4741 acgaggaaag aggcgcgcag ggcggccacc tggtcagcgg gcggaacgcg gccgttgacc
     4801 tcacgggcct cgatgtggac ggtctcgtcc tcgatggtga cgtccgcgcc gagcgagcgc
     4861 aggacctcgg ccatctcgcc gatgtccgcg atggcgggga cgcggcgcag ggtgacgggc
     4921 tcagcggtga ggaggccggc ggcgagggcg tagagggagg cgttcttgga gccggagacg
     4981 gtgacgcgcc ctcgaagggg gcgtccgccg cggatgacga ggcgtgtggc ggtcttggtc
     5041 gtcacgaggc gaggataacg gacgaggcga gatatggggg cgggtggatg ctcgaatgcc
     5101 gcctcgcgta ttcggggagg aggtgacgat gtccaaggtg gtgctcggca aagaagaccg
     5161 cgaggacgca gtgcgccggc tgcgcgcgta cttcgagcgg gagcgaggcg aggagctggg
     5221 tgagctcggc gcgacgttgc tgttcgagtt cctcgcggag gagatcgcgc cgcactttta
     5281 caacagggga ctggctgatg cgcagtcact cgtagcgcgg ttcacggact cactcgacgc
     5341 ggacctcgag gcggcgaaag tgtttccgcc ccgggcgtag ttggccctcg gcggttcacg
     5401 cgggccggcg gacgcggtag tggaggtcca cgacgccgca gttgaaccgg cgctcgtgga
     5461 ggagttcgag gtcgaggcgg aagtccctcg gaagggcggg tttaccagca ccgacggcga
     5521 cggggtgcag cacgaggtgg cattcatcga tgaggccggc ccggaaggcg tgggcggcca
     5581 gctcggggcc gccgatggcg aggtcggcgg atgactcggc tttgaggcgg cggacggcct
     5641 cggcgtcgaa agtgcgttcg gtgcgggtgc gggcggtgag gggcgcctcg agggtggtgg
     5701 agtagacgat cttgt
//
LOCUS       DAORPG010000038         5712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_790_length_5712_cov_3.602263, whole genome shotgun sequence.
ACCESSION   DAORPG010000038 DAORPG010000000
VERSION     DAORPG010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_790_length_5712_cov_3.602263"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="PLX85_01595"
     CDS             complement(<1..709)
                     /locus_tag="PLX85_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRC61901.1"
                     /translation="MASTFYTLQGANRRNSLLLMLSVAAILGLLGFAIGYGSTGQLSA
                     AIVVTTIAVIVAGFLSLISYYQGDSIVLAASKARQVTEADAPQLLNIVRELSIAANLP
                     MPKVYEIDDSAPNAFATGRDPQHASIAVTTGLLEKLNREELQGVIGHELGHVGNYDIR
                     FALLVGVLVGSIALMADVFLRWTWWSGFGGRGRSRDRDGGGAGQAIIFVIAIVLAILA
                     PIFARMVQLAVSRQREYL"
     gene            complement(731..1297)
                     /locus_tag="PLX85_01600"
     CDS             complement(731..1297)
                     /locus_tag="PLX85_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRC61902.1"
                     /translation="MSLIVILVIVAVVVVVALILIGIYNGLVRKRLRVDEAWAQIDVQ
                     LKRRHDLIPNLVNAVKDYMGFEQEVLTQVTQARANAIAAGAQGPQQAAAAENQLTGAL
                     RSLFAVVENYPDLKANQNVLSLQEELTTTENQISFSRQHYNATVYEYNTSTATFPNAI
                     LAGMFGFRPREFFDAEPEAMEVPTVSLR"
     gene            complement(1356..2270)
                     /locus_tag="PLX85_01605"
     CDS             complement(1356..2270)
                     /locus_tag="PLX85_01605"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRC61903.1"
                     /translation="MKLRRGALAWTASPVLAVSVLIAVYGALGGRVHPEPSEAGVAVV
                     ASEAAATEAPTPTGTVPPALAVVTAPSPTPTSSPPIATPTATPSPSRVERSGSARVLD
                     RGPATRPFVALTFDAGSDAGYTAQILDVLHAQGVTASFGITGRWAEQNPELLRRIVRE
                     GHHLINHTYDHDSLTGLSTNAAPMTRTQRWSELDRTADAVRQIAGVDIGPYFRPPYGD
                     YDASVNADVYARGYDYNVMWTVDSLGWRGISADEIVARCLANAQPGAIYIFHVGSASQ
                     DGNALDRIITGLRRGGYGMGSVPQLLGS"
     gene            complement(2267..3196)
                     /locus_tag="PLX85_01610"
     CDS             complement(2267..3196)
                     /locus_tag="PLX85_01610"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRC61904.1"
                     /translation="MVAGRASLWLALAVAFPLIGIACSRGGASSVVATLAAAPATRTS
                     PAAAAAITATPTPTSTPTKRPPTVVIDPGHGADEIGAAANSIVEKESNLEMALRVERL
                     LSAQGVRVILTRRADVHANSGPPVTGYTATRLDLQARIDLANAEQADLFVSIHSNGST
                     NAADRGIESYYNSSRPFVAQNQALASALLRNVIAELRAGGFDVRERGALDDACLRAFQ
                     GRCFPLFVLGPGRETTRDEAIRRGGDPAALGFAPGQDSITSRATQMPGALIELLFITN
                     PLDAAILQTDAAREAMERGIVRGIMERLPEVAR"
     gene            3319..4101
                     /locus_tag="PLX85_01615"
     CDS             3319..4101
                     /locus_tag="PLX85_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61905.1"
                     /translation="MPLDRVARRFLDRHRLARLASSAAEGVPAILPVYFAIDPAVEDV
                     LYAVPAPPVRVPVLAASPFEAALLVDDLDEVRGRTTGTLLRGAAAPCANEAEFAAAMS
                     ALATRYPDWHVAPAEGVPLLRLRIQHAETWGRLEAPAEAGSDTAGLQLVRTVERIAAP
                     HITTEHRTGVSSGGVARLRRAGDDMVEEPVESFDEAAMPDEGGRMLRYPLDHLDDGLY
                     VADSVGPANTLRRTFFEVIDGRVGRLFDDRRRALRELRHLDR"
     gene            4209..4925
                     /locus_tag="PLX85_01620"
     CDS             4209..4925
                     /locus_tag="PLX85_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HRC61906.1"
                     /translation="MPNEQSSVWIRLLAGALAVVVLSFIVSQIASGEVIPPLLIQAVI
                     FTALAYAVWRWQRRRWVLIVAIVLTVLGLAGGIPFMVEDLAHPESAWVFVPSSVVIIG
                     LLVAIVSGGAALFRRSEAIARPLAIGAGALAVVLVVVGVVATLGVSDDARADGDTVIV
                     AKKASYPSTVTVRAGDVGLYIENKDLIRHTFVIETADVKVELPGSASRHLAVALKPGA
                     YDFRCDVPGHDSMKGTLEVK"
     gene            complement(4982..5374)
                     /locus_tag="PLX85_01625"
     CDS             complement(4982..5374)
                     /locus_tag="PLX85_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007428165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC61907.1"
                     /translation="MPRVVHFEIHADDVERAIGFYRAVFAWEFPEWMPGEYWGVMTAA
                     EGSSEPGINGGLMRRQGSAPTSGQAVNSYVCTVQVEDIDGCLAAVTAAGGATVVAKHA
                     LTGLAWQAYCTDTEGNIFGVHQPDESAA"
BASE COUNT          842 a         2165 c         1773 g          932 t
ORIGIN      
        1 cgaggtactc ccgctggcgg ctcaccgcca gctgcaccat ccgcgcaaag atcggtgcga
       61 ggatcgcgag gacgatcgcg atcacgaaga tgatcgcctg gcccgcgccg ccgccatcgc
      121 ggtcccgtga tcgcccccgg cccccgaacc cgctccacca cgtccagcgc aggaagacat
      181 ccgccatcaa cgcgatgctg cccacgagca ccccgacgag gagcgcgaag cggatgtcgt
      241 agttcccgac gtgacccagt tcgtgcccga tcacgccctg cagctcctcg cggttcagct
      301 tctcgaggag cccggtcgtc acggcgatcg aggcgtgctg cggatcgcgt cccgtcgcga
      361 acgcgttcgg cgcgctgtca tcgatctcgt acaccttagg catcggcagg ttcgccgcga
      421 tcgacagttc gcgcacgatg ttcagcaact gcggcgcgtc cgcctcggtc acctgccgcg
      481 ccttcgaggc ggccagcacg atcgagtcgc cctggtagta ggagatgagc gacaggaacc
      541 ccgcgacgat gaccgcgatc gtcgtcacga cgatcgcggc ggacagctgc ccggtcgatc
      601 cgtagccgat cgcgaagcca agcaacccga ggatcgccgc cacggacagc atgagcagca
      661 gcgaattccg tcggttcgcc ccctgcagtg tgtagaaggt gctggccacg gccgccccga
      721 ccgccgccgc ttagcggagg ctgaccgtcg ggacctccat cgcctccggc tccgcatcga
      781 agaactcgcg cgggcggaac ccgaacatcc ccgcgaggat cgcgttcggg aacgtggcgg
      841 tcgacgtgtt gtactcgtac accgtcgcgt tgtagtgctg ccgcgagaac gagatctggt
      901 tctccgtcgt cgtcagctcc tcctgcagcg acagcacgtt ctggttcgcc ttcaggtccg
      961 ggtagttctc gaccaccgcg aacagggagc gcagcgcgcc ggtcagctgg ttctctgctg
     1021 ccgccgcctg ctgaggcccc tgcgcccccg ccgcgatcgc gttcgcgcgt gcctgcgtca
     1081 cctgggtgag cacttcctgc tcgaacccca tgtagtcctt gaccgcgttc acgaggttcg
     1141 ggatcaagtc gtgccggcgc ttcaactgca cgtcgatctg cgcccacgcc tcgtccaccc
     1201 gcagccgctt gcggaccagc ccgttgtaga tgccgatgag gatcagcgcc acgaccacga
     1261 cgaccgcgac gatcaccagg atgacgatca ggctcataac gcctcctccg aggagtcccc
     1321 tatcttccga gtatacgtcc gccccacccg cgcacctagc tgccgagcag ctgcgggacc
     1381 gaccccatcc catatccgcc ccgtcgtagc cccgtgatga tgcggtcgag agcgttccca
     1441 tcctgcgagg ccgaaccgac gtgaaagatg tagatcgccc ccggctgtgc gttcgccagg
     1501 caccgcgcca cgatctcatc cgccgagatg ccccgccagc ccagtgagtc gaccgtccac
     1561 atgacgttgt agtcgtagcc gcgcgcatat acgtcggcgt tgacggaggc gtcgtagtct
     1621 ccgtacggcg ggcggaagta cggcccgatg tcgacccccg cgatctgccg caccgcgtcc
     1681 gccgtccggt ccagttcgct ccagcgctgc gtgcgcgtca tcggcgcggc gttcgtcgag
     1741 agtcccgtga gcgagtcatg gtcgtacgtg tgattgatga ggtgatgtcc ctcgcgcacg
     1801 atgcgccgga gcaattccgg gttctgctca gcccatctcc ccgtgatccc gaagctggcg
     1861 gtcaccccct gcgcgtgcag gacatcgagg atctgcgccg tatacccagc gtccgaccca
     1921 gcgtcgaacg tcagtgccac aaaggggcgt gttgctggcc ccctgtccag cacccgcgct
     1981 gatcccgagc gttccactct cgagggcgaa ggtgtcgcgg tcggagtcgc gatcggcggc
     2041 gaactggtgg gcgttggcga tggcgccgtc accaccgcga gcgctggcgg aacggtcccc
     2101 gtcggcgtcg gcgcctcggt cgcggccgcc tcgctcgcaa caacggccac gccagcctcc
     2161 gagggctcgg ggtgcaccct cccgccaagg gcgccgtaca ccgcgatcag cactgagacc
     2221 gccaggacgg gcgaggccgt ccatgccagc gcgccccgcc gcagcttcaa cgtgccacct
     2281 ccggcaatcg ctccatgatc ccgcgcacga tgcctcgttc catcgcctct cgtgccgcgt
     2341 ccgtctgcag gatcgcggcg tcgagggggt tcgtaatgaa caacagctcg atcaatgcgc
     2401 ccggcatctg ggttgcgcgg ctcgtgatcg agtcttgccc cggcgcgaac ccgagcgcag
     2461 ccgggtcgcc gccacgccgg atcgcctcgt cgcgtgtcgt ctcgcgcccg ggtccaagca
     2521 cgaacagcgg gaagcaacgg ccctgaaacg cccgcaggca cgcatcgtcc agcgccccgc
     2581 gttcgcgcac gtcgaatccg cccgcccgca actccgcgat cacgttccgc agcaaggcgg
     2641 acgccagcgc ctggttctgc gccacgaacg gacgcgacga gttgtagtac gactcgatcc
     2701 ctcggtccgc cgcgttagtt gacccgttcg agtgaatcga gacgaacagg tcggcctgct
     2761 ccgcgttcgc caggtcgatg cgcgcctgca ggtcgagccg cgtcgccgtg tagcccgtga
     2821 ccggtggtcc gctgttggcg tgcacgtccg cgcgcctcgt gaggatgacc cgcaccccct
     2881 gcgccgagag caaccgctcc acccgtagcg ccatctcgag gttcgactcc ttctccacga
     2941 tgctgttggc cgctgccccg atctcgtccg ccccgtgacc agggtcgatc accacggtcg
     3001 gcggccgctt cgtcggggtc gaggttggcg taggagtcgc ggtaatggcg gccgccgccg
     3061 ccggcgaggt gcgggtcgca ggtgccgcgg caagcgtcgc gaccacactc gacgcgcctc
     3121 cccgtgaaca tgcgatgcca ataagtggga acgcgaccgc gagggcgagc cacaggctgg
     3181 cccgtcccgc gaccatgaac gaatggatcg ctcgaggttg ccccacaccc gcaacgcgca
     3241 ctgcaaacca tggtagaccg ctgcgggagc gtcgcaagcg gactgcggta agccacggcg
     3301 gccctctaca atccgcccat gcccctcgac cgcgtcgccc gtcgctttct cgatcgtcac
     3361 cgcctggccc gcctcgcctc cagcgccgcc gagggcgtcc cggcgatcct gcccgtgtac
     3421 ttcgccatcg accccgcggt cgaggacgtg ctgtacgcgg tccctgcgcc gccggtccga
     3481 gtgccagtgc tcgccgcgtc gcccttcgag gccgccctcc tcgtcgacga cctcgacgag
     3541 gtccgtggtc gcaccaccgg cacgctcctg cgaggcgcgg ccgccccctg cgccaacgaa
     3601 gccgagttcg ccgccgcgat gtccgcgctg gccacccgct accccgactg gcacgtcgcc
     3661 cccgccgagg gggttccact tcttcggctt cgcatccagc acgccgagac ctggggccgc
     3721 ctcgaggcgc ctgccgaggc tggctccgac actgccgggc tccaactcgt ccgcactgtc
     3781 gagcgcatcg ccgcccccca catcaccacc gagcaccgca ccggcgtcag ctccggtggc
     3841 gtcgctcgcc tccggcgcgc cggcgatgac atggtggagg agcccgttga gtcgttcgat
     3901 gaagccgcca tgcctgacga gggggggcgc atgctccgct accccctcga ccacctcgac
     3961 gacggcctct acgtcgcgga cagcgtcggc cccgccaaca cgctccgccg caccttcttc
     4021 gaggtcatcg acggccgcgt cggccgactg ttcgacgacc gtcgccgcgc tctccgcgag
     4081 ctccgccatc tcgatcgctg acccggctcg ctggtgaacg agacctgacc tggctggccc
     4141 cgctgcacat tcgcatgcgg ttgtaatcgg cctctcgtaa tatcccaatg caacgagagg
     4201 tggtcggagt gcccaacgag caatcatccg tgtggattcg gctgctggcc ggcgccctgg
     4261 cggtcgtcgt tctgagcttc atcgtgtctc agatcgccag tggcgaggtg atcccgcccc
     4321 tgctcatcca ggcggtcatc ttcaccgcgc tcgcttacgc ggtatggcgc tggcagcgcc
     4381 ggcgctgggt gctgatcgtt gccatcgtcc tcacggtcct tggcctcgcc ggaggcatcc
     4441 cgttcatggt cgaagatctc gctcaccccg agtcggcctg ggtgttcgtg ccatccagcg
     4501 tggtcatcat cggcctcctc gtcgcgatcg tctcgggcgg cgctgccctg ttccgccgct
     4561 ccgaggcaat cgcgcgcccg ctggccatcg gggcgggggc cctggcggtc gtgctggtcg
     4621 tagtcggtgt cgtcgccacg ctgggcgtga gcgacgatgc ccgggcagac ggcgacacgg
     4681 tgatcgtcgc gaagaaggcc tcgtatccat cgaccgtcac ggtcagggct ggcgacgtag
     4741 gcctctacat cgaaaacaag gacctcatcc ggcacacctt cgtcatcgaa actgccgatg
     4801 tgaaagtcga gctgccgggt tcggccagcc ggcacctagc ggtcgcgctc aagcccggcg
     4861 cctacgactt ccgctgcgat gtgcccggtc atgattcgat gaagggcacc ctggaggtga
     4921 agtagcgcgt actcgtcgca tcgaggagcg cgcgcgactg cgcgcccctc gatgcgtagg
     4981 atcacgccgc cgactcgtcc ggctggtgca cgccgaagat gtttccctct gtgtcggtgc
     5041 agtacgcctg ccacgccagc cccgtcagcg cgtgcttcgc caccacggtc gcgccacccg
     5101 ccgccgtcac ggccgcgagg cagccatcga tgtcctccac ctgcaccgtg cagacgtacg
     5161 agttcaccgc ctggcccgag gtcggcgcgg acccctgccg ccgcatcaac ccgccattga
     5221 tccccggctc cgaggaccct tccgccgccg tcatcacccc ccagtactcg cccggcatcc
     5281 actccgggaa ctcccacgcg aataccgccc ggtagaaccc aatcgcccgc tccacgtcat
     5341 cggcgtggat ctcgaaatgc accacccgag gcaccagcac tgctcctctc cggccgcgca
     5401 ccgcttgcgc cgccccctcg agcctaccct gcgctccgcc ccttcgtacc cctcgatgtc
     5461 actcgctcgc gctccgggca tccgaggcgc accggccgcc gctggccctg cgcggcgcgc
     5521 aagcgctgtt cctcgtgccg ctgggcgtgt tccagctcac cgcggtcacc gtcttcacgg
     5581 tcgccgacca cgtccacgga atcgagctgc tcgtcagcgc ctgggccgcc gtcatggccg
     5641 tggccggcat cgccctcgga ctccgactcg ctcgactgca cggcccgcgc gtccgagggc
     5701 tcgcgttcgg cc
//
LOCUS       DAORPG010000039         5664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_802_length_5664_cov_3.287930, whole genome shotgun sequence.
ACCESSION   DAORPG010000039 DAORPG010000000
VERSION     DAORPG010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_802_length_5664_cov_3.287930"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2177
                     /locus_tag="PLX85_01630"
     CDS             <1..2177
                     /locus_tag="PLX85_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HRC61908.1"
                     /translation="VRGDVVALAAGDIVPADGVLVGGDEVTLDLSMITGETVPVGRTP
                     EVASAEPEAHVSEVPAIAPAGAAVVTGSATALVWATGPTSTLGQIASLVESVQRGEST
                     LERQVRALSWTTAVIAVLTGTGTLALATAFTDTSFVSALTFATGVIVALVPEGMLPTL
                     SVALAIGARRMAERGAAVRRLAAVEIVGSATVICTDKTGTLTENVLSVLGVDGVDGQA
                     VRASTRAGLAAGLCNNARIEGDGRFTGDPIDVALAEWMRVGGVDLEALRARSPRLSET
                     PFDAHLRLMTTTHLVDGHATEFIKGAPEAVLATLETEPTPALRAALDAAAEAGERVLL
                     LASRPEGSPARIEGLVRFFDPPRHEVPGAIAACRAAGIGVVMLTGDHPATARFVASQV
                     GLEGDGGLPVIEGATLDRMGDQAVMRVLRGNAIFARIHPEQKLRIVRLLRQSGAVVVV
                     TGDGINDAPALRAADVGVAMGRRGTEVAKQAADIVLADDNFATLVAAIEEGRSLKRNI
                     RRFVSYVFTSNVAEVMPFLCYVFLQVPLPLAVIQALAIDVGTDLAPALALGAEPPAAA
                     TMQSAPELPGRPLMTRRLAMRTFLVFGLIEAALGLAAYLAFYAHAGWRPFADLDDLLV
                     HEASTLTFLGIVGGQVGCLFAQRDGSLRRRLSFRSNRLLGIGLASELLLAPVLVYVPG
                     LNQTFQMAPVPAPWLLAIPVCAIAFLGFDLLRRSIEGRAEEG"
     gene            2274..2747
                     /locus_tag="PLX85_01635"
     CDS             2274..2747
                     /locus_tag="PLX85_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRC61909.1"
                     /translation="MYRKILMTHDGSELASEAVAHVVELARLGQSEVLLLRVTDTPGQ
                     TLTRLGGGWAAAGEVAAEAAVEETQRHDTEAVAQLSDLREQLHARGVERVSIEVVEGA
                     PGPAIIEVAAQQGCDLIVMATHGRSGLGRAVLGSVADHVIRHSSIPVLLVRPASA"
     gene            complement(2744..3229)
                     /locus_tag="PLX85_01640"
     CDS             complement(2744..3229)
                     /locus_tag="PLX85_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRC61910.1"
                     /translation="MYRRIMLTLDGSELSRSAIPQAIALVQGSSTEVVVFQAIDTVAA
                     LRNRFIGESFEFTGGRPEAIAELAESQHFMQRDRAHEELEAAAEQLRAVGAAVTTEVG
                     EGVPGNAILDAADRHDVDAIVMATRGHGGLGRDVVGSVAEYVLRHAGNRALILAGSRS
                     G"
     gene            complement(3320..3514)
                     /locus_tag="PLX85_01645"
     CDS             complement(3320..3514)
                     /locus_tag="PLX85_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61911.1"
                     /translation="MGDFLWGFWNGLTAWPLLIAHAFHWWERFPVYNVGRDSGWYQFG
                     FLIGAGSLFAGPARGSRRRR"
     gene            complement(3478..4164)
                     /locus_tag="PLX85_01650"
     CDS             complement(3478..4164)
                     /locus_tag="PLX85_01650"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HRC61912.1"
                     /translation="MDLCKIDFKREARELYAPPRHPVLVDVPRFKFLMVDGCGPATGS
                     PDFEAAVGLLYTASYSLKFSLKRTLGLDWIVAPLEGLWSSGGSPAFDPGRPADWTWTL
                     MIRQPEQVTAAHVEVVRAQVAAKARGSLLERLRLEDLAEGRCAQVMHIGPYSTEAPAV
                     DALKAFIAAQCLAPAGRHHEIYLSDPNRTAPERIKTILRQPVCARENVWAPSEGEQAW
                     AISSGVSGTA"
     gene            complement(4258..5283)
                     /locus_tag="PLX85_01655"
     CDS             complement(4258..5283)
                     /locus_tag="PLX85_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HRC61913.1"
                     /translation="MDSVLLQALVDLAPDGILLVHPSGRIVFVNGQAERLFGYQADEL
                     LGRTVDELVPARFRATHHQHQAAYHAHPVARPMGTGLELFAERSDGSELPVEISLGSI
                     PTEEGVYVTAIVRDMTERRALEEERRQLVAQAELQRDRERIARDLHDGVMQSIYAVGL
                     SLIETRTTLEAAAPEAVPALDEAVGELRQVIDDIRRYVMGLSLERLDSDVTTLLREVI
                     AETHAGSDLEVALDVADDLPALTDALTQAVYHLAREALTNVRKHSGAHHASVALTTAA
                     DGVLLEVRDDGCGFDTGAAVERGHMGLRNMRDRVASLGGSIELRSEPGSGTAVLVHVP
                     LEAPASA"
     gene            5437..>5664
                     /locus_tag="PLX85_01660"
     CDS             5437..>5664
                     /locus_tag="PLX85_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate CoA-transferase"
                     /protein_id="HRC61914.1"
                     /translation="MSASEALEAVQSGQRVYLHGGAATPTPLLFALADRARRLSGVET
                     VSLHLDGPAPHVAPELAGHLRHNALFIGANVR"
BASE COUNT          833 a         1897 c         2073 g          861 t
ORIGIN      
        1 tcgtgcgcgg cgacgtggtg gcgctggcgg cgggggacat cgtgccggcc gacggcgtgc
       61 ttgtcggggg cgacgaagtg acactcgacc tctcgatgat cacgggcgag accgtcccgg
      121 tcgggcggac gcctgaagtc gcctccgcgg agcccgaggc gcacgtgagc gaggtgccgg
      181 cgatcgcgcc agcgggcgct gccgtcgtca ccgggtcagc aaccgcgctg gtttgggcga
      241 ccgggccaac gagcaccctc gggcagatcg cgtcgctcgt cgaaagtgtg cagcgaggcg
      301 agtcgaccct cgagcggcag gtgcgcgcgt tgtcctggac gaccgccgtg atcgccgtgc
      361 tcaccgggac ggggacgctt gccctcgcca cagcgttcac ggacacgtca ttcgtgtcgg
      421 cgctcacctt tgcgacgggc gtcatcgtgg cgctcgtgcc ggagggcatg ctgcccaccc
      481 tgagtgtggc gcttgcgatc ggcgcgcggc ggatggccga acgcggcgcc gcggtacgtc
      541 ggctcgcggc ggttgagatc gtcgggtccg cgacggtcat ctgcacggac aagaccggca
      601 ccctgacgga gaacgtcctg tccgtcctcg gcgtggacgg ggtcgatggg caggcggtcc
      661 gcgccagcac ccgcgcgggc ctggcggccg gcctgtgcaa caacgcacgc atcgagggcg
      721 atggacgctt caccggcgac ccgatcgacg tcgcgctggc cgaatggatg agggtgggcg
      781 gcgtggacct cgaagcgctg cgtgcgcgat cgccgcggct cagcgagacg cccttcgatg
      841 ctcacctccg cttgatgacg accacccatc tcgtcgacgg gcacgccacc gagttcatca
      901 agggcgcgcc cgaggcggtg ctggccacac tggagaccga acccaccccg gcgcttcggg
      961 ccgcgctcga cgcggccgcc gaggcgggcg agcgcgtgct cctcctggcg agccggccgg
     1021 agggctctcc cgcacgcatc gaggggttag tgcgcttctt tgacccaccg cgccacgaag
     1081 tgccgggagc gatcgcggcc tgccgggccg caggcatcgg cgtggtgatg ctcacgggag
     1141 accatccggc gaccgcgcgc tttgtggcct cgcaggtcgg gctcgagggc gacggaggcc
     1201 ttcccgtgat cgagggcgcg accctcgacc ggatgggcga ccaggcggtg atgcgtgtgc
     1261 tgcgcgggaa cgcgatcttc gcgcgcattc atccggagca gaagttgcgc atcgtcagac
     1321 tgctgcgtca gtcgggcgca gttgtggtgg tgacgggaga cggcatcaac gacgcccccg
     1381 cgctgcgcgc cgccgacgtc ggcgtcgcca tgggccggcg aggcaccgag gtggccaagc
     1441 aggcggcgga catcgtgctc gccgacgaca acttcgccac cctcgtcgcg gcgatcgagg
     1501 aggggcgctc cctgaagcgg aacatccggc gcttcgtgag ctacgtgttc acgagcaacg
     1561 tcgccgaggt gatgccgttc ctctgctacg tcttcctgca ggtgccgctg ccgctggcag
     1621 tgatccaggc gctcgccatc gacgtgggga cggacctcgc accggcgctg gcgctcggtg
     1681 cggaaccacc ggcggcagcc accatgcagt cggcgccgga gttgccgggc cggcccctga
     1741 tgacgcggcg gctcgcgatg cgaaccttcc tcgtgtttgg cctcatcgag gcggccctcg
     1801 ggctggccgc ctacctcgcg ttctacgcgc acgctggctg gcgcccgttc gcggacctcg
     1861 atgacctgct cgtgcacgag gcgtccacgc tgaccttcct tggcatcgtg ggcggacagg
     1921 tcggatgcct gttcgcacaa cgggacggct cgttgcggcg gcgcctgtcg ttccgctcga
     1981 accggctgct cgggatcggg ctcgcctcgg agctgctgct ggcccccgtg cttgtctacg
     2041 tccccggcct caaccagacg ttccagatgg cgccagtgcc ggcgccatgg ctgctcgcta
     2101 tccccgtctg cgctatcgcg tttctcggat tcgatctcct gcgccggtcc atcgaggggc
     2161 gcgccgagga gggatagcgc gggacgcatc ccgagggccg ggggacgttc ggcggtgcga
     2221 gccgctcggt cgagcgcgat gatggcaact gcggacggga gaggagtgag tgcatgtacc
     2281 ggaagatcct gatgacccac gacggctcag agctggcgtc cgaggcggtc gctcatgtgg
     2341 tggagctcgc ccgcctcggg cagtccgagg tcctcttgct gcgcgtcacg gacacgccgg
     2401 gacagaccct gacgcgcctc ggcggcgggt gggcagcggc tggtgaggtc gcggccgagg
     2461 cggcggtcga agagacgcag cgacacgaca cggaggccgt ggcgcagctg tcggatctac
     2521 gcgaacagct gcacgctcgc ggcgtcgagc gcgtgtcgat cgaagtcgtc gagggagcgc
     2581 ccggacccgc gatcatcgag gtcgccgcac agcaggggtg tgacctcatc gtgatggcga
     2641 cgcacgggcg atccggactc ggccgagcgg tgctgggatc ggtggcggac cacgtgatcc
     2701 gccactcctc gatcccggtg ctgctggtgc ggccggcctc cgcctagccg gagcgcgaac
     2761 ccgcgaggat cagcgcgcgg ttgcccgcgt ggcggagcac gtactcggcg accgacccga
     2821 cgacgtcgcg gccaaggccg ccgtgcccgc gagtcgccat cacgatggcg tccacgtcgt
     2881 ggcggtcggc cgcgtcgagg atggcgttgc caggcacccc ttcaccgacc tcggtcgtga
     2941 cggcggcgcc gacggcgcgg agttgctcag cggcggcttc gagttcctcg tgggcgcggt
     3001 cacgctgcat gaaatgctgg ctctcggcga gttcggcgat cgcctcgggg cggccgcctg
     3061 tgaactcaaa cgactcgcca atgaagcggt tacgcaacgc cgcgaccgtg tcgatcgcct
     3121 ggaacacgac gacctcggtg ctcgagccct ggacgagcgc gatggcctgc gggatcgctg
     3181 aacgggacag ctcggaacca tccagcgtca gcatgatgcg tcgatacatc cgatcctcct
     3241 tcgactgcaa cgagatcgtg cagccctcgg cagcggccgg gcaggtgcat tcgtcatgcc
     3301 tcgaggatga cgaacggcgt cagcggcggc ggcgggagcc acgggcgggg ccggcgaata
     3361 acgaaccggc gccaatcagg aagccgaact ggtaccagcc cgagtcgcgg ccgacgttgt
     3421 agaccgggaa ccgttcccac cagtggaagg cgtgcgcaat gagcagcggc caggccgtca
     3481 ggccgttcca gaaaccccag aggaaatcgc ccatgcctgc tctccttccg acggcgccca
     3541 cacgttctca cgtgcgcaaa caggctgccg aaggatcgtc tttatgcgct cgggggcggt
     3601 gcggttgggg tcactcaggt agatctcgtg gtggcggccg gccggcgcca ggcactgcgc
     3661 tgcgatgaag gccttcaggg cgtcgaccgc cggggcctcc gttgagtacg gcccgatgtg
     3721 catcacctgg gcacagcggc cctcggccag gtcttcgagg cggagccgtt cgagcagcga
     3781 gccgcgcgcc ttcgccgcaa cctgcgcccg cacgacctcg acgtgcgcgg ccgttacctg
     3841 ctcgggctgc cggatcatca acgtccaggt ccagtccgcg gggcggccgg ggtcgaaggc
     3901 cgggctgccg ccggatgacc agagcccctc gaggggggcg acgatccagt cgaggccaag
     3961 cgtgcgcttc aggctgaact tcaggctgta cgaggctgtg tagaggagac ccacggccgc
     4021 ctcgaagtcg ggtgagcctg tagcggggcc gcaaccgtcg accatgagga acttgaacct
     4081 cgggacgtcg accagtaccg ggtgacgcgg cggcgcatac agctctctgg cctcgcgctt
     4141 gaagtcgatc ttgcagagat ccacgttggc ctcctctgcg cgcgcgatca gggtgcgcgc
     4201 ccgcgtcggg cgcgggaggt gccataggtc acgagggaat cgggactcgg cgggcggtca
     4261 ggcggaggcg ggcgcctcga ggggcacgtg gacgagcacg gcggtgccgg agcccggttc
     4321 gctccggagc tcgatcgacc cgcccaacga cgcgacacgg tcgcgcatgt tgcgcaggcc
     4381 catgtgcccc cgctcgacgg ccgcgccggt atcgaacccg cagccatcgt cgcgcacctc
     4441 gagcaggacg ccatccgcag cggtggtgag cgcgacgctg gcgtggtggg cgcccgagtg
     4501 tttgcgcacg ttcgtcagcg cctcgcgggc gaggtgatag acggcctggg tcaacgcgtc
     4561 ggtcagagcc gggaggtcgt cggcaacgtc cagggcgacc tcgaggtcag atccggcgtg
     4621 ggtttccgcg atcacctcgc gcagcagcgt agtgacgtcc gaatcgagcc gttcgagcga
     4681 cagccccatg acgtagcggc ggatgtcatc gatgacctgg cgcagctccc caaccgcctc
     4741 gtcaagagcg gggacggcct cgggcgcggc ggcctcgagc gtcgtgcgcg tttcgatgag
     4801 gctgaggcca acggcataga tcgattgcat gacgccgtcg tgcaggtctc gggcgatgcg
     4861 ttcgcggtcc cgctgcagtt cggcctgggc cacgagctga cggcgttcct cctcgagcgc
     4921 ccgacgctca gtcatgtccc gcacgatcgc ggtgacgtag acgccttctt cagtaggaat
     4981 ggacccgagg ctaatctcga cgggcagttc gctgccgtcg ctgcgctcgg cgaacagctc
     5041 gaggccggtg cccatcggcc tcgcgacggg gtgcgcatga taggccgcct ggtgctggtg
     5101 atgcgtagcg cggaaacgcg ccggcaccag ctcgtcgacc gtgcggccga ggagttcgtc
     5161 cgcctggtag ccgaagagac gctcagcctg gccgttcacg aacacgatcc ggccgcttgg
     5221 gtggacgagg aggatgccgt ccggggcgag gtcaaccagg gcctgaagca ggacgctatc
     5281 cacgatggcg gttcccggtg tgcagttgcc cgatggtgct ctgccacgag tgtaccggct
     5341 gccgacggca agcacccggc gtctccgcgg cggctcgtgt agggtgtgcg cggagagctt
     5401 gccccgccga ggaaaggcca ccgtgccatg aggtccgtgt ccgcgagcga agcactggag
     5461 gcggtgcaga gtggccagcg cgtgtatttg cacggcggcg ccgcgacgcc cacaccgctg
     5521 ctattcgcgc tcgcggaccg cgctcgtcgt ctcagcggag ttgagaccgt ttcgctgcac
     5581 ctcgacggtc ccgcgccgca cgtggcaccg gaactggcgg ggcacctgcg acacaacgcg
     5641 ctgttcatcg gcgcgaacgt ccgg
//
LOCUS       DAORPG010000040         5620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_818_length_5620_cov_3.397844, whole genome shotgun sequence.
ACCESSION   DAORPG010000040 DAORPG010000000
VERSION     DAORPG010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_818_length_5620_cov_3.397844"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..748
                     /locus_tag="PLX85_01665"
     CDS             5..748
                     /locus_tag="PLX85_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HRC61915.1"
                     /translation="MLGLPAGIRACLFDLDGVLTDTASVHAAAWKATFDTLLRVRAQA
                     TGDAFVPFDAVEDYRRYVDGRPRLDGVRTFLAARGITLPEGSTTDGPSAATVAGVGTR
                     KNELVLAALQAHAVPAYEGSIAYVRAARDAGLRCAVVSSSANTRPVLASAGIETLFDV
                     YVDGLVAAERRLRGKPAPDTFLAAAEALGVPAAQAAVFEDALVGVQAGRAGAFGYVVG
                     VDRAGQADALREHGADIVVRDLRELLGAT"
     gene            745..3123
                     /locus_tag="PLX85_01670"
     CDS             745..3123
                     /locus_tag="PLX85_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HRC61916.1"
                     /translation="MITHECFTSDPWCIRECGLDLEVLAQTESIFALSNGHIGLRGNL
                     DEGEPVGIPGTYLNGFYELRPLPYAEGGYGYPESGQALINVTNGKLLRLLVDDEPFDI
                     RYGTLHNHERVLDLRAGTLQREVEWESPAGQRVKVSSTRLVSFTQRAVAAISFEVEAV
                     GAAARVVVQSELVANEPLAAPTGDPRVAEALRSPLVAEDDAARDPGRVVLVHHTAGSG
                     LRMAAAMDHLVEAPQKVDVSAETSPDIGRVTVTTVLAPGERLRIVKFLAYGWSGVRSR
                     QALRDQVAAALTAASYTGWEGLLEEQRAYLDAFWDDALIEVEGDPTVEQAARFALFQM
                     LQASARAERRAIPAKGLTGQGYDGHAFWDSETFVLPVLIYTAPEVAAHELRWRHSTLP
                     IARQRARALGLAGAAFPWRTIAGAECSGYWPAGTAAFHINADIAAAVVWYMEATEDEA
                     FMREAGLELLVETARLWRSLGHHDRTGQFRISGVTGPDEYSALQNNNVYTNLMAQQNL
                     SAAADAVQHHEAEAKALGVDQEEAASWRDAARDMFIPYDPELGVHPQADGYTTLEVWD
                     FEATRPDQYPLFLHFPYFDLYRKQVLKQADLVLAMQLRGDAFTFQEKQRNFAYYEPLT
                     VRDSSLSAATQGVLAAEVGQLDLAYDYLRETALLDLGDLHDNTRNGLHLAALAGVWSL
                     LVAGCGGMRCQQGQIGFAPRVPPGLVRLAFRVRFRGRHLQVEVTSGEARYELRSGPPL
                     DLLHHGQRFTLSERTPTVLAIPPLPPMARPSQPKGREPLHVRPGPEGTSYPS"
     gene            complement(3111..3533)
                     /locus_tag="PLX85_01675"
     CDS             complement(3111..3533)
                     /locus_tag="PLX85_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRC61917.1"
                     /translation="MYEKLLYPVDGSDLSLVALPHVAEIARASGATVTVLQAVEIVTE
                     ADAITVPERRGEADESIHRIVASLRDQGVTVAAPRVIEGDAGRSIVEAADADAADLVI
                     MATHGRSGLRRLALGSVADYVVGHASCPVLLIRPPQDG"
     gene            complement(3577..4575)
                     /locus_tag="PLX85_01680"
     CDS             complement(3577..4575)
                     /locus_tag="PLX85_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HRC61918.1"
                     /translation="MLAQLLRRPRSIDLAPLTIEAIEAPAPGPGELRLRLEACAVCRT
                     DLQIVEGDLALHRAPLVPGHQAVGIVEAIGPGVEGWAAGDRAGVGWLGDVCGACEYCT
                     TGRENLCESATFTGWDRDGGYAQAMLVRAGFALRLPAALEAEALAPLLCGGVIGYRSL
                     RVSGIGPGQRLGLYGFGASASLAIQVAVHMGCEVFVVTRSTEEQRRARALGAAWAGTL
                     DEQPPARLHAAVTFAPVGEVVLAALRALAPGGTVAINAIHLDRVPEFSYDWLWRERSL
                     RSVANFTRADAREFVALAAEIPLRTETQPFALAEANEALRALRQGELRGTAVLMIE"
     gene            4655..5317
                     /locus_tag="PLX85_01685"
     CDS             4655..5317
                     /locus_tag="PLX85_01685"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRC61919.1"
                     /translation="MTAVIFDLDGTLVDSVYQHVVAWQAGLRAAGIELAVWRIHRRIG
                     MSGGLLAHALLREAGRDLDDATLRRVQDVHREEYLARVGSIRPLPGAVELLGYLTSEA
                     VPWTVATSGRLTTARPVLDLLPAPGTGLLVTRDDVSRAKPDPDLFIEAARRLGQPVED
                     SVIVGDSVWDLLAARRAHALGVGLLSGGYGRDELQAAGAYRVYDDPADLLDHLDEVGI
                     RR"
     gene            complement(5328..>5620)
                     /locus_tag="PLX85_01690"
     CDS             complement(5328..>5620)
                     /locus_tag="PLX85_01690"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC61920.1"
                     /translation="NADEQGATSRTFVQAYKAAYGAAPQLFAAQGYSSVYLLLDAAKR
                     ASAPTPAALRDALAATRDLDTPLGRLSMSDKREATHPPVTQQYRNGQLVPLP"
BASE COUNT          810 a         2029 c         1969 g          812 t
ORIGIN      
        1 aatcgtgctc ggccttcccg cgggcatccg cgcctgcctc tttgacctcg acggcgtgct
       61 gacggacacg gcttcggtgc acgccgctgc ctggaaggcc actttcgaca cgttgctgcg
      121 ggttcgcgct caggcgacgg gcgacgcctt cgtcccattc gacgccgtcg aggattaccg
      181 acgctacgtg gacggccgtc ctcggctcga tggggtgcgc acgttcctgg ccgctcgcgg
      241 catcacgctg cccgaaggca gcacgaccga tggtccttcg gccgcgacag tggccggcgt
      301 cggcacacgc aagaacgaac tcgtgctggc cgcgctccag gcccacgccg tccccgccta
      361 cgaggggtcc atcgcgtacg tccgcgccgc gcgcgatgcc gggctgcgct gcgcggtcgt
      421 ctcttcgagc gccaacacga ggccggtgct ggcgtccgcc ggcatcgaga ccttgttcga
      481 cgtgtacgtc gatggcctcg tcgctgccga acgccgcctg cgtggcaagc ccgcgccgga
      541 cacgttcctc gccgcggcgg aagccctcgg ggtgccggcc gcacaggccg ccgtcttcga
      601 agacgccctg gtgggtgtcc aggcggggcg cgccggagcg tttggatacg tcgtcggggt
      661 agatcgcgca ggacaggcgg acgctctacg cgagcatgga gctgatatcg tggtacgcga
      721 cctgagggag ctgctgggcg cgacatgatc acgcacgagt gcttcacgtc cgacccgtgg
      781 tgtatccgcg agtgtggcct cgaccttgaa gtgctcgcgc agaccgagtc gatcttcgcg
      841 ctctcaaacg ggcacatcgg cctccgcggc aacctcgatg agggcgagcc agtcggcatc
      901 cccggcacct atctgaacgg cttctacgag ctgcgcccgc ttccgtacgc tgagggcggc
      961 tacggctatc ccgagtcggg ccaggccctg atcaacgtca ccaacgggaa gctgctgcgg
     1021 ctgctggtcg acgatgagcc gttcgacatc cgctatggca cgctccacaa ccacgagcgc
     1081 gtcctcgacc tgcgggccgg cacactgcag cgcgaggtcg aatgggagtc gcccgcggga
     1141 cagcgggtga aggtatcgtc gacacggctg gtgtcgttca cgcagcgcgc ggtcgccgcc
     1201 atcagcttcg aggtggaagc ggtcggcgcg gcggcgcgcg tcgtcgtgca gtcggaactc
     1261 gtggcgaacg agccgctcgc ggcgccgacc ggcgacccgc gcgtggccga agcattgcgc
     1321 tcacccctcg tcgccgagga cgatgccgcc cgcgatccgg ggcgagtcgt cctcgtacac
     1381 cacactgccg gaagcggtct gcgcatggcc gccgcgatgg accacctcgt ggaggccccg
     1441 cagaaggtag atgtttcggc ggagacctcg cccgacatcg ggcgggtcac ggtcaccacg
     1501 gtgctggcgc ccggagagcg gctgcgcatc gtgaagttcc tggcctacgg ctggtcgggc
     1561 gtgcgctctc gacaggcgct gcgcgaccag gtcgccgcgg cgctgaccgc ggcgagctac
     1621 acgggctggg aaggcctgct cgaggagcag cgagcctacc tcgatgcctt ctgggacgat
     1681 gcgctcatcg aggtggaagg cgacccgact gtcgaacagg ccgctcgctt cgcgctcttc
     1741 cagatgctgc aggccagcgc ccgggcggag cggcgcgcca tcccggcgaa gggcctgacc
     1801 gggcagggct acgacgggca cgcattctgg gacagcgaga cgttcgtgct gcccgtcctg
     1861 atctacaccg cacccgaggt cgcggcgcat gagctgcgct ggcgccactc gacgttgccg
     1921 atcgcgcggc agcgcgcccg cgcgctcggg ctcgcggggg ccgccttccc ctggcggacc
     1981 atcgcgggcg ccgagtgctc gggatactgg ccggccggga ccgcggcgtt ccacatcaac
     2041 gcggacatcg cggcggccgt cgtctggtac atggaggcga ccgaggacga ggcgttcatg
     2101 cgcgaggcgg gcctcgagct gctggtggag acggcgcgcc tctggcgctc gctcggccac
     2161 cacgaccgca ccgggcagtt ccgcatctcg ggcgtgaccg gcccggacga gtacagcgcc
     2221 ctgcagaaca acaacgtcta cacgaatctc atggcccagc agaacctctc ggcggcggcg
     2281 gacgccgtgc agcaccacga ggcggaggcg aaggcgctcg gcgtcgacca ggaggaggcg
     2341 gcgtcgtggc gtgacgcggc gcgcgacatg ttcatcccct acgaccccga gctcggggtg
     2401 catccgcagg ctgatggata caccaccctc gaggtctggg acttcgaggc gacgcgcccg
     2461 gaccagtacc cgctgttcct gcacttcccg tacttcgacc tctaccggaa gcaggtcctg
     2521 aagcaggccg acctcgtcct ggcgatgcag ctccgcggcg atgccttcac cttccaggag
     2581 aagcagcgca acttcgcgta ctacgaaccg ctgacggtgc gcgactcctc cctgtccgcc
     2641 gccacgcaag gcgtgctcgc ggcggaggtg gggcagctcg acctggccta cgactacctg
     2701 cgggagacgg ccctgctcga ccttggcgac ctgcacgaca acacccggaa cggcctgcac
     2761 ctcgcggcgc tcgcgggcgt ctggtcactg ctcgtagcgg ggtgcggcgg catgcgctgc
     2821 cagcaaggcc agatcgggtt cgccccgcgc gtgccgccgg gcctcgtgcg cctcgcgttc
     2881 cgcgtccgct tccggggacg tcacctgcag gtggaggtga cctcgggaga ggcgcgctac
     2941 gagctgcggt ccgggccacc gctcgatctg ctgcaccacg gtcagcgctt cacgctgagc
     3001 gagcggaccc cgacggtgct cgcgattccg ccgctgccgc cgatggcgcg gccgagccag
     3061 ccgaagggcc gcgagccgct ccacgtgcgg cccggccccg aggggacgag ctacccgtcc
     3121 tgaggcggcc ggatgagcag caccgggcac gatgcgtgcc caaccacgta gtccgcgacc
     3181 gagccaagcg cgagcctgcg cagcccggat cgcccgtgcg tcgccatgat cacgaggtcc
     3241 gctgcatcgg cgtccgcggc ctcgacgatc gagcgcccgg cgtcaccttc gatcacacgc
     3301 ggtgcagcga ccgtcacccc ctggtcccgc aggctcgcca cgatgcggtg aatggactcg
     3361 tctgcctcgc ctcggcgctc cggcaccgtg atcgcgtcgg cctcggtcac gatctcgacg
     3421 gcctgcaaca ctgtcacggt cgcgcccgag gcgcgcgcga tctccgcgac atgtggcaac
     3481 gcgacgaggg acaggtccga gccgtcgacc gggtagagca gcttctcgta catcgcttcc
     3541 ccgcatccca tccggacgac cgaggtgacc gcccgcctac tcgatcataa gcacggccgt
     3601 gccgcgcagc tcaccctgcc gcagcgctcg cagcgcctcg ttcgcttcag ccagcgcgaa
     3661 cggctgcgtc tcggtccgca gcgggatctc cgctgcgagc gcgacgaatt cgcgcgcgtc
     3721 tgcccgcgtg aagttcgcca cgctgcgcaa actgcgctct cgccacagcc agtcgtacga
     3781 gaactctggc acgcggtcga ggtggatggc gttgatcgcc accgtgcccc cgggcgcgag
     3841 cgcgcgcaac gcggcaagga cgacctcccc aaccggggcg aaggtcaccg ccgcgtgcag
     3901 tcttgccgga ggttgctcgt cgagggtgcc ggcccatgca gcgccgaggg cacgcgctcg
     3961 ccgttgctcc tcggtggagc gcgtcacgac gaagacctcg catcccatgt gcacggccac
     4021 ctggatcgcc agcgacgcgg acgcgccgaa gccgtacagc cccagccgct ggcccggtcc
     4081 gattcccgac acccgcagcg agcgatagcc gatgacgccg ccgcacagca acggcgccag
     4141 cgcttcggct tcgagggcgg ccggcaggcg gagcgcgaag ccggcccgca cgagcatggc
     4201 ctgcgcataa ccgccgtcgc ggtcccatcc cgtgaaggtc gcggactcac agaggttctc
     4261 gcggccggtg gtgcagtact cacaggcgcc gcagacgtcg cccagccatc cgacgccggc
     4321 acggtcaccc gccgcccagc cctccacgcc cgggccaatc gcctcgacga tgcctaccgc
     4381 ctggtggccg ggcaccagcg gcgctcgatg cagcgcgagg tcgccctcaa cgatctgcag
     4441 gtcagtgcgg cagaccgcgc acgcctcgag gcgcaggcgt agctctccgg ggccgggcgc
     4501 cggcgcttcg atcgcctcga tcgtgagtgg cgcgaggtcg atcgaacgcg ggcgtctcag
     4561 cagctgagcc agcatcgttg tcggcctcct cggaccgcag ggtacctcgg gtgcgcgcgg
     4621 cagaccggga tcggccgggc gtatcgtgga agcgatgaca gcggtgatct tcgacctcga
     4681 tgggacactg gtcgacagcg tgtatcagca cgtggtcgcc tggcaggccg gcctgcgcgc
     4741 ggccggcatc gagcttgccg tctggcgcat tcaccggcgg atcggcatga gcggtggcct
     4801 cctcgcccac gccctcctcc gcgaggcggg acgcgacctc gatgacgcga cgctgcggcg
     4861 ggtgcaagac gtgcatcgcg aggagtacct cgcgcgcgtt ggctccatcc gccccctccc
     4921 cggcgcggtc gaactgctgg ggtacctgac ctcggaggcg gtgccctgga cggtcgccac
     4981 gagcggccgc ctgacgacgg cccgcccagt cctcgacctg ctgccggcgc ccgggaccgg
     5041 cctgctggtg acgcgggacg atgtgtctcg cgccaagcca gaccccgacc tcttcatcga
     5101 ggcagcgcgc cgcctcggac agcccgtcga ggacagcgtg atcgtggggg acagcgtctg
     5161 ggacctgctg gcggcgcggc gggcgcacgc gctcggggtc gggctgctct cagggggcta
     5221 cggtcgcgac gagctgcaag cagcgggcgc ctaccgcgtc tatgacgacc cggcggacct
     5281 cctcgaccac ctggacgagg tcggcatccg ccgctgagcc acgggcccta cgggagcggc
     5341 acgagctggc cgttgcggta ctgctgcgtc acgggcgggt gggtcgcctc gcgcttgtct
     5401 gacatcgaca gacgaccgag cggcgtgtcg aggtcgcgcg tagcggcgag ggcgtcccgc
     5461 agcgccgcgg gtgtcggcgc gctcgcgcgc tttgctgcgt cgaggagcaa gtacacactg
     5521 ctgtagccct gcgcggcgaa cagctgcggc gcggcgccgt aggcggcttt gtaggcctgc
     5581 acgaaggtgc ggctcgtggc cccctgctca tcggcgttcc
//
LOCUS       DAORPG010000041         5604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_824_length_5604_cov_3.257704, whole genome shotgun sequence.
ACCESSION   DAORPG010000041 DAORPG010000000
VERSION     DAORPG010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_824_length_5604_cov_3.257704"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..589)
                     /gene="liaF"
                     /locus_tag="PLX85_01695"
     CDS             complement(26..589)
                     /gene="liaF"
                     /locus_tag="PLX85_01695"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HRC61921.1"
                     /translation="MSWRAKDVLPLSIAAYLVALGGLYLLDSLGIHSYGVAGSIGVAV
                     GLAFIWAGILAFIAAWRVRRFARRLRRAIGHVRSDLAGWAVDDAVVSTVLGDISLDLR
                     QADLPEGETQLTLLCWLGTIQVRVPDDVGLDVTAQTMVGSVEVLGRREEGFVRDIHVR
                     SEAYEGQPKRLQMRLSTFVGELLVVHG"
     gene            complement(603..764)
                     /locus_tag="PLX85_01700"
     CDS             complement(603..764)
                     /locus_tag="PLX85_01700"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HRC61922.1"
                     /translation="MAVGVVLVVIGTIFLLEALGVLDVGINELWPVILIAIGLVIVYE
                     RMRRYYRRR"
     gene            1089..1817
                     /locus_tag="PLX85_01705"
     CDS             1089..1817
                     /locus_tag="PLX85_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC61923.1"
                     /translation="MTNNASATTDSIIQVDGLVKNYDTGAVQVQALRGVSLSVRRGEM
                     VAVMGPSGCGKTTLLNCLSGIDDVTSGRVLIEGTDISTLDDNTKTSYRARRMGFIFQF
                     YNLLPVLTAVENVELPLLVSGTSPKESRQRAIEALRRVGLEEWAQHKPGQLSGGQRQR
                     VTIARALVNRPAIVWADEPTGDLDSATANDIMGLMQQLNREQTLTFVIVTHDPSIAAR
                     CHRIIHMQDGRIEREVVNQEARVG"
     gene            1810..5226
                     /locus_tag="PLX85_01710"
     CDS             1810..5226
                     /locus_tag="PLX85_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRC61924.1"
                     /translation="MDKLFGIQLNSIMVALLILLAVVFAVIVWIGIRRPLLVRMGLRN
                     AIRRPAQTALIVVGLMLSTLIISAAFSTGDTVGYSITNLVYGSLQGVDYLVGFDREKN
                     GVEREQAYLTTSFLDSVRQQFGNDPDVDGITPLLVEQLPILNESQRLSQPQGAFIGVD
                     PGSVASFHALRDLDDNEVSADALTGNRTYISDRLSSQVNARAGDTLTVFVENKPTTFE
                     VIGVVRDTSITSRQAFDGGSGSEPPGGMIVNLQTAQQILDRSGRVSYVGVSTAGGTRD
                     ALALRSDRVEQKLDDFIDANPDSRAKVIASKHDLVSLAEIIGSVFVTFFIVFGLFSIA
                     AGIMLIFLIFIMLAAERRSEMGMARAVGMSRLNLTESFLAEGMAYNIGSAAVGAVLGL
                     GVSALLVFILARTFNDSGFNITFHFNPRAFVIAYSLGVVLTFVTVTFSAYRAANLNIV
                     RAIRDIDEPQMLRGPSRSISSLLLSAVGVLWYLAWLGLVIVLALGAAALFFLGLSTYG
                     LGLLAAGALVGLFWFGARLVNVRSRRSVGQWVLLVLWAVAFNVIALLTWVLLKTRAWA
                     GHYRNAGGWALWMLIGGVVLTYLGGWVWHQQFAYTGGTTLAVLAVAMLAVYFGTPSRP
                     AFTIASIALVWYWLLPLPFSLFTDDPLEHFGPLEGLTKLLGLPQPESDGNIEMFFVSG
                     ICITASATLLVIFNADALLAIVSAFGRLLGGIAPALKTAIAYPLASKFRTGITLAMFG
                     LVVFSLVVMATLNSNFTQLFAGDEAKAGFDVMVEGNPSNRIPDLRVALRDAGYQGPAL
                     AGVGTQLVASTFGAVVQRDAAPKQDILPISVVGDDDEFLRIAKLPMKYRAAGYETDEA
                     IIQALRNDPTVAVVDDSLISGTNDFGGPASSDQFDLSGVIKAEGFDPVAVTVKNPRGG
                     DDLHLKVIGTLEPQVTGILQQLAGLHVNRQNVDRLFGGGDAETFFVKLEGSPSKRQSV
                     DAANAIESALLERGVQASSIQERIDDSSSQSTAFQYLFEGFMGLGLVVGIAALGVIAF
                     RTVVERRQQIGMLRAIGYSRRLVALSFFFESSFIALAGVAMGLLLGGALSYNLLTSPT
                     FTSGATIEFHFPWIRILVICAIAYGASALMTLIPARAASRVAVAEALRYE"
     gene            5294..>5604
                     /locus_tag="PLX85_01715"
     CDS             5294..>5604
                     /locus_tag="PLX85_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61925.1"
                     /translation="MSNPLTRAAAAALLVLTLVLSLNSSASAQSAGEIGGNVPQNGGF
                     AIVVWGGGTPAALVQAATAKGCSPASIWITAQGQFVPYVVGAPDFVNDGFVGRFTGGN
                     MP"
BASE COUNT          942 a         1872 c         1781 g         1009 t
ORIGIN      
        1 cccgctccgc gcctgacgcc ggcccttacc cgtggacgac gagcaactcg cccacgaacg
       61 tcgagagccg catctgcagc cgcttcggct gtccctcgta cgcctcgctg cggacgtgga
      121 tgtcacgcac gaacccctct tcgcggcgac cgagtacctc gactgacccg accatcgttt
      181 gcgccgtgac gtcgaggccc acgtcgtccg gcacgcgtac ctggatcgtg ccgagccagc
      241 acagcagcgt gagctgtgtc tcaccctcgg gcaggtccgc ttgccgcagg tcgagcgaga
      301 tgtcaccgag cactgtggag acgacggcgt cgtcgaccgc ccagcccgcg aggtctgagc
      361 gtacgtggcc aatggcccgg cgcagccgcc gcgcaaagcg ccgcacacgc cacgcggcga
      421 tgaaggcaag aatccccgcc cagatgaacg cgaggccgac ggcgaccccg atcgagcccg
      481 cgacgccata ggagtggatg ccgaggctgt cgagcagata gagaccgccg agcgcgacca
      541 ggtatgcggc gatcgagagc ggaaggacat ccttggcgcg ccagctcatg gggcctgact
      601 gctcatcgtc tgcggtagta gcgccgcatc cgctcgtaca cgatgacgag gccgatggcg
      661 atcaggatca cgggccacag ctcgttgatc cccacgtcga ggactcccag cgcctcgagc
      721 aggaagatcg tcccgatgac cacgagcacc acaccgacgg ccacggagcc cccccgagac
      781 caccaacact agattccgcc gcgagtcccc ctctggagag accaccactt ccgcgcccgg
      841 acgcgccgca agcctcgcgg ccgctttcgc tccgatcccg gtggcgaaat acctctgccg
      901 ggggacgacg cgggacagcg ccctcatcat tgtatggtta tgagcctctg tgtaccttgg
      961 aagcgtcgct cgccgtgccc cgctggagca cgtcttcatt ccgttcccgc tcggatcgct
     1021 cgatcttcac tcgctccgag atctccgagc cgcactccac atcgacgctc gcaacgaacg
     1081 ggatcccgat gaccaacaac gctagcgcca ccacggattc gatcatccag gtcgatggcc
     1141 tggtgaaaaa ctacgacacc ggcgcagtcc aggtgcaggc tctacgaggc gtctcgctct
     1201 ccgtccggcg gggcgagatg gtcgccgtga tggggccctc cggctgcggc aaaacgacgc
     1261 tgctcaactg cctgtccggc atcgacgacg ttacgagcgg gcgcgtgctc atcgagggca
     1321 ccgacatcag cacgctcgat gacaacacga agacttcgta ccgcgcgcgg cgaatgggat
     1381 tcatcttcca gttctacaac ctgctgccgg tactcacggc ggtcgagaac gtcgagttgc
     1441 cgctgctggt ctcgggtacg tctccgaaag agtcgcgtca gcgcgcgatc gaagccctgc
     1501 gtcgggtggg cctcgaggag tgggcccagc acaagcccgg acagctctcg ggaggtcagc
     1561 ggcagcgcgt gacgatcgcg cgagccctcg taaaccgtcc ggccatcgtc tgggcggacg
     1621 agcccacggg agacctcgac tcggcgaccg ccaacgacat catggggctt atgcagcagc
     1681 tgaaccgcga gcagaccctg accttcgtca tcgtgacgca cgatcccagt atcgccgcgc
     1741 ggtgtcaccg catcatccat atgcaggacg gacggatcga gcgcgaggtc gtcaaccagg
     1801 aggcgcgcgt tggataagct ctttgggatc caactcaaca gcatcatggt ggcgctgctg
     1861 atcctgctcg ccgtcgtctt cgcggtcatc gtctggatcg gcatccggcg accgctgctc
     1921 gtacgcatgg ggctgcgcaa tgcgattcga cgtcccgcac agacggcctt gatcgtcgtt
     1981 gggctgatgc tttcaaccct gatcatcagt gccgccttct caacagggga caccgtcggc
     2041 tactcgatca ccaacctcgt gtacggctca ctgcagggtg tcgactacct cgttggcttc
     2101 gaccgggaga agaacggcgt cgagcgcgaa caggcctacc tcaccaccag ctttctcgac
     2161 tcagtccgtc agcaattcgg caacgacccc gacgtcgacg gcatcacccc cttgctggtc
     2221 gagcagctgc cgatcctgaa cgagtcgcag cgtctctcgc agccacaggg cgccttcatc
     2281 ggcgtcgacc ctggctcggt tgcgagcttc catgctctcc gcgacctcga cgacaacgag
     2341 gtgagcgccg acgccctcac ggggaatcgg acgtacatca gcgaccgcct ctcgagccag
     2401 gtaaatgcgc gggcgggcga cacactcacc gtcttcgtcg agaacaagcc cacgacgttc
     2461 gaagtgatcg gggtcgtcag agacacctcg atcacgtcac ggcaggcgtt cgacggtgga
     2521 agcgggtcgg agccgccggg cggcatgatc gtgaacctcc agacggcgca gcagatcctc
     2581 gatcgcagcg gtcgcgtctc ctacgtgggc gtctccaccg ctggcggaac tcgcgatgcc
     2641 ctcgcgttgc gctccgaccg agtcgagcag aagctcgacg attttatcga cgcgaatcca
     2701 gacagccggg cgaaggtgat cgcgtcgaag cacgacctgg tcagcctggc cgagatcatc
     2761 gggtccgtct tcgtcacctt cttcatcgtg ttcgggctct tctcgattgc cgccggaatc
     2821 atgctgatct tcctgatctt catcatgctg gccgccgagc gacgctcgga aatggggatg
     2881 gcccgcgcgg tagggatgag ccggctgaat ctgaccgaga gcttcctcgc cgaagggatg
     2941 gcctacaaca tcggatcggc ggctgtggga gccgtgctcg gtctgggcgt gtccgcactg
     3001 ctggtgttca tcctcgcgag gaccttcaac gactccggtt tcaacatcac cttccacttc
     3061 aacccgcgcg cgttcgtcat cgcatacagc ctcggcgtcg tcctcacctt cgtgacggtc
     3121 acgttttccg cctaccgggc ggcgaacctg aacatcgtgc gcgcgatccg cgacatcgat
     3181 gaaccgcaga tgctgcgcgg ccccagtcgc tccatctcga gcctcctgtt gtcggccgtc
     3241 ggcgtgctct ggtacctcgc gtggctgggg ctcgtcatcg tgctcgcgct tggcgccgct
     3301 gcactcttct tcctggggct gagcacctac ggcctgggac tcctcgcggc gggcgcactc
     3361 gtcggtctgt tctggttcgg agcccggctc gtgaacgtgc gaagccgacg atcagtgggg
     3421 cagtgggtgc tgctcgtgct gtgggcggtg gcattcaacg tgattgcgct cctgacctgg
     3481 gtactgctca aaacccgcgc ctgggcgggg cactaccgca acgcgggagg ctgggccctc
     3541 tggatgctca tcggcggagt cgtgctgact tacctcgggg ggtgggtctg gcaccagcaa
     3601 ttcgcgtaca cgggcgggac caccctcgct gtgctcgcgg tcgcgatgct cgccgtctac
     3661 ttcgggacgc catcgcggcc ggcgttcacc atcgcgagca tcgcgctggt gtggtactgg
     3721 ctgttgccgc tgccgttctc gctcttcacg gatgaccccc tcgagcactt tgggccgctc
     3781 gaagggctga cgaagctgct ggggttgccg cagcccgagt ccgacggcaa catcgagatg
     3841 ttcttcgtct cggggatctg catcacggca tcggccacgc tgctcgtcat cttcaacgcg
     3901 gacgcgctgc tggcgatcgt gtccgcgttc ggccggttgt tgggaggcat cgcgcccgcg
     3961 ctgaagacgg ccatcgccta cccactcgcc tcgaagttcc gcaccgggat cacgctggcg
     4021 atgttcggcc tcgtcgtatt cagcctcgtg gtgatggcca ctctcaactc gaacttcact
     4081 cagttgttcg cgggcgacga ggccaaggct ggattcgacg tgatggtcga aggcaatccg
     4141 tcgaaccgga ttcccgattt gcgggtggcg ctgcgcgacg ccggctacca gggaccggcc
     4201 ctggccggtg tggggacaca gttggtcgcc tcgacgttcg gcgccgtggt acagcgggat
     4261 gcggcgccga agcaggacat ccttcccatc tcggtcgtgg gcgacgatga cgaattcctg
     4321 cgcatcgcca agctcccgat gaaataccga gccgcggggt atgagaccga cgaggcgatc
     4381 atccaggcac tccgcaacga cccaacggta gccgtggtcg atgacagcct gatcagcggg
     4441 acgaacgact tcggggggcc tgcctcatcc gaccagttcg acctgtcggg cgtcatcaag
     4501 gctgagggat tcgatccggt cgccgtcacg gtgaagaacc cccgaggtgg agatgacctg
     4561 cacctgaagg tgatcggaac gctcgagcca caggtgaccg ggatcctcca gcagctcgcg
     4621 gggttgcatg tgaaccggca gaacgtcgac cgcctgttcg gcggcggcga cgcggagacg
     4681 ttcttcgtga agctcgaggg gagcccctcg aagcggcagt cagtggatgc cgcgaatgcc
     4741 atcgagtcgg cgttgctgga gcgaggcgtc caggccagct ccattcagga gcgcatcgac
     4801 gactcgtcct cccagtcgac tgccttccag tacctgttcg aggggttcat ggggctcggc
     4861 cttgtggtcg gcatcgccgc cctcggcgtc atcgccttcc ggacggtcgt cgagcggcgg
     4921 cagcagatcg ggatgctgcg cgcgatcggc tactcgcgtc gcctcgtcgc actttcgttc
     4981 ttcttcgagt catcgttcat cgcgctcgcg ggggtggcga tgggactgct gctcggggga
     5041 gcactgtcgt acaacctgct gacgagtcca acgtttacca gcggcgcgac catcgagttc
     5101 catttcccgt ggattcgaat cctcgtcatc tgcgccattg catacggcgc gagtgcgctg
     5161 atgaccctga tccccgcccg agccgcgtct cgggtcgcgg tcgcggaggc gttgcgctac
     5221 gaataacacg aaacggtatg tacgccttag cgaaaacgcg ctaccctacc gctcgctgat
     5281 gagaggagca cttatgtcga accccctcac gcgggcagcg gcggcggcat tgctggtact
     5341 caccctggtg ttgagcctca actcgagcgc gtccgcgcag tcggccggtg agatcggcgg
     5401 gaacgtcccg cagaacggcg gctttgcgat cgtggtctgg ggcggcggga cgccggcggc
     5461 gctggtgcag gcggcgaccg caaagggctg ttctccggcc tcgatctgga tcaccgcgca
     5521 agggcaattc gtcccgtacg tcgtgggcgc ccccgacttc gtgaatgacg gcttcgtggg
     5581 ccgcttcaca ggcgggaaca tgcc
//
LOCUS       DAORPG010000042         5565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_843_length_5565_cov_3.448094, whole genome shotgun sequence.
ACCESSION   DAORPG010000042 DAORPG010000000
VERSION     DAORPG010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_843_length_5565_cov_3.448094"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /gene="glgX"
                     /locus_tag="PLX85_01720"
     CDS             <1..1393
                     /gene="glgX"
                     /locus_tag="PLX85_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HRC61926.1"
                     /translation="IAPGNQVLEFKQMVRALHAAGIEVILDVVYNHTAEGNHLGPMLS
                     FKGIDNAAFYRLVADDPRYYMDYTGTGNTLNMRNPFVLQLIMDSLRYWVTEMHVDGFR
                     FDLAATLARELHDVDRLSAFFDLVQQDPIVSQVKLIAEPWDVGDGGYQVGNFPPQWAE
                     WNGKYRDTVRDFWRGEDQTLADFANRFTGSSDLYQNSRRYPSASVNFVTAHDGFTLRD
                     LVSYNEKHNEANGEENRDGESHNRSWNCGVEGDTDDPEVLGLRARQQRNFLATLFLSQ
                     GIPMLLHGDEAGRTQSGNNNAYCQDNQTSWFDWSAVDESLLEFTRRLIAFRRDHPVFH
                     RRRWFQGRPIRGVGHDIAWYKPGGGEMTDEDWDTGFAKSLLVYLNGEGIAAPDEWGEH
                     VHDSSFLVCFNAHHEDIDFTLPDVEPGLRWTEALSTLPDAAEPAPTPAASAAPITVAP
                     RSILVLIGVPAEA"
     gene            1771..3570
                     /locus_tag="PLX85_01725"
     CDS             1771..3570
                     /locus_tag="PLX85_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRC61927.1"
                     /translation="MSDFVSPEARLFAIIAVAIALLVSERIRADLVGVLIVVALWAAR
                     LLNPDEALAGFSSEPAIAVAAVFVLGAGLLETGVSDAAGRLIARWSGPGLLRPLALLM
                     GSVGLLSAFTHHVTTTASMVPVALDLSKERDIPPSRLLMPISFAASLGTTITIIGAPA
                     FLIASDALQGAGEPPLGVFSVAPLGLALLAAGVLFMLLTGRWLLPDRRGVEDPSAGYR
                     LDGYLTEIEVQPGSPLLDKSAEDLATDRRYEFEVVGRIRDGSRVPGRFAEGDLQEGDV
                     LLVRTSPEHMLAVGEERGIELGPTSQYERASVPRDADEEDVEERLVQVVVAAASDFAG
                     RTIAQVDFRQRFGVLVLGLWRQRGWLADQLAHVRLQPGDVLVVQGDEDALARLSADRG
                     VLMLVPFQGRARVLRKRWLALAILLGAIALAIAGAPLQMAMLVGAAAMVLTRCVTPGQ
                     AYRAIDQRVFVFIAGAIPLGAAMEKTGAARTIAELLEQPLSGATPFIALLAIFVAVAL
                     VTQFMSDAATTALFAPIALGLAGAIGVSPTACVVTVAVGSVASSITPLGHHGNLLVYG
                     PGGYRFSDFVRAGTPLTLLVGVIAAAGTPLLWP"
     gene            3687..4376
                     /locus_tag="PLX85_01730"
     CDS             3687..4376
                     /locus_tag="PLX85_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRC61928.1"
                     /translation="MARSGDTTTDLVHRVPLLASLSEEDIRSLAPIAGRRSFRHGHTI
                     FREGEPGDSLYILIRGSVFITMLSSDGAESILAVLRGGDCLGELSLLDGFPRSATARA
                     AEDVDALVVTRDHFQSWLADHHAASLALLRTLSMRIRRTNAALADRNALDLWQRLAKQ
                     LLLLADDQPRTSASDGPDLRVTQQQLAEMLGVTREAVNKTLREFAQAGWIELRRGLVR
                     ICDAASLAEQL"
     gene            complement(4776..>5565)
                     /locus_tag="PLX85_01735"
     CDS             complement(4776..>5565)
                     /locus_tag="PLX85_01735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HRC61929.1"
                     /translation="EPAVVGELIAELNERFPRHPAIDSYLVATGRLPDWTDVAAVGRG
                     QALFERESAAVMTVLGFGALPESYCNAAVARELNRHSTLDVDPLRRVRRTAEFVSSVM
                     SPGGLGDGADGRRAIQRVRLLHAALRLSGEPGTRPESPSGEARLNQEDLAATLLTLSW
                     MTAIRLPRVGVQVDARTLYDYAHSWNVAGSLLGIDERLLPASADAAAELAEAIRRRRY
                     QATPEGAALANALLDIEEVWAPAGDLRALVRQLLGHELAAMIAV"
BASE COUNT          819 a         2191 c         1665 g          890 t
ORIGIN      
        1 catcgcgccc ggcaaccagg tcctcgagtt caagcagatg gtgcgcgctc tgcacgccgc
       61 cggcatcgag gtcatcctcg acgtcgtcta caaccacacc gccgagggca accacctcgg
      121 cccgatgctt tccttcaagg gcatcgacaa tgccgccttc taccgcctcg tcgccgacga
      181 cccgcgctac tacatggact acaccggcac cggcaacacg ctgaacatgc gcaacccctt
      241 cgtgctccag ctcatcatgg acagcctccg ctactgggtg accgagatgc acgtcgacgg
      301 cttccgcttc gacctcgcgg cgaccctcgc tcgcgagctc cacgacgtcg accgcctctc
      361 tgccttcttc gacctcgtcc agcaggaccc gatcgtgagc caggtcaagc tcatcgccga
      421 gccatgggat gtcggcgacg gcggctacca ggtcggcaac ttcccgcccc agtgggccga
      481 atggaacggc aagtaccgcg acacggtccg cgacttctgg cgaggcgagg accagaccct
      541 cgccgacttc gccaatcgct tcaccggcag ctccgacctc taccagaaca gccgccgcta
      601 cccctccgcc agcgtcaact tcgtcaccgc ccacgacggc ttcacactcc gcgacctcgt
      661 gtcctacaac gagaaacaca acgaggccaa cggcgaggag aatcgtgacg gcgagtcgca
      721 caaccgctca tggaactgcg gcgtcgaggg cgacacggac gaccccgagg tgctcggcct
      781 ccgcgcacgc cagcagcgca acttcctcgc cacgctcttc ctctcccagg gcattccgat
      841 gctcctccac ggcgacgagg ccggtcgcac ccagtccggc aacaacaacg cctattgcca
      901 ggacaaccag acctcgtggt tcgactggtc cgccgtcgat gagtccctgc tcgagttcac
      961 ccgccgctta atcgccttcc gccgcgatca tcccgtcttc caccgtcgcc gctggttcca
     1021 gggccgtccc attcgaggcg tcggccatga catcgcctgg tacaaacctg gtggcggcga
     1081 gatgaccgac gaggactggg acaccggctt cgccaagtcg ttgctcgttt acctcaatgg
     1141 cgaaggcatc gccgcccccg acgagtgggg cgagcacgtc cacgactcca gcttcctcgt
     1201 ctgcttcaac gcccaccacg aggacatcga cttcaccctc ccggacgtcg agcccggcct
     1261 ccgctggacc gaggcgttga gcacgctgcc agacgccgcc gaacccgccc ccacgcccgc
     1321 cgcttcggcc gcgcccatca ccgtcgcccc ccgcagcatc ctcgtgctca ttggcgtccc
     1381 tgccgaggcc tgagcatcgg cgcgctcgcc tcgccggcgc ggcgtctgca attcctgcgg
     1441 cgggcgctgt tcctcgtgcg caacgccttt gcccgtagcc tccgaagcaa gcccacgcag
     1501 gaggagacat gaacgacaac gaccagcaga agcagctaaa gatggccggc ggcgccggcc
     1561 ggcatggcga ccggcggctt cctcggctgg tcgatgggca tgatgagcgg ctccatgatc
     1621 ggattcgtcg ccggcctctg gaccggcctc atgattcgca agggcacgaa gcacgggcaa
     1681 cggccacacg atgggcgagc gcgtcatcgg cactcacggt ctcgggcgcg ccccggtcgc
     1741 acccatgggg gatgaacgcg ccgcgctgac gtgagtgact tcgtttctcc cgaggcgaga
     1801 ctattcgcca tcatcgcggt cgcgatcgcg ctgctcgtca gcgagcgcat tcgcgcggac
     1861 cttgttgggg tcctcatcgt ggtcgccctc tgggccgccc gccttctcaa ccccgacgag
     1921 gcgctcgccg gcttcagcag cgagccggcc atcgctgtcg ccgccgtgtt cgtcctcggc
     1981 gcgggcctcc tcgagaccgg cgtctccgat gccgctggcc gcctgatcgc ccgctggtca
     2041 ggccccgggt tgcttcgccc gctcgccttg ctgatgggct cggtcggcct cctctccgcc
     2101 ttcacacacc acgtcacgac taccgccagc atggtccccg tcgcgctcga cctctcgaag
     2161 gaacgcgaca tcccgccctc gcgcctgctg atgccgatct cgttcgccgc ctcgctcggc
     2221 accaccatca ccatcatcgg cgcccccgcc ttcctcatcg cgagcgacgc gctccagggc
     2281 gccggcgaac ctccgctcgg tgtcttctcc gtcgcccccc tcggcctcgc gctcctcgcc
     2341 gccggcgtgc tcttcatgct cctcaccggc cgctggctgc tccccgaccg ccgaggcgtc
     2401 gaagacccgt ccgccggcta ccgcctcgac ggctacctca ccgagatcga ggtccagccc
     2461 ggctcgccgc tcctcgacaa gtctgccgag gatctcgcca ccgaccgccg ctacgaattc
     2521 gaggtcgtcg gccgcatccg agacggctcg cgcgtccccg gccgcttcgc cgagggtgac
     2581 ctccaggaag gcgatgtcct cctcgtccgc acgtccccgg agcacatgct cgccgtcggc
     2641 gaggagcgcg ggatcgagct cggccccacg agccagtacg agcgcgcttc ggtccccaga
     2701 gacgccgacg aagaggacgt cgaggaacgc cttgtccagg tcgtcgtcgc cgccgcctcg
     2761 gacttcgctg gccgcaccat cgcccaggtc gacttccgtc agcgcttcgg tgtcctcgtc
     2821 ctcggtctct ggcggcagcg cggctggctc gcggatcagc tcgcccacgt ccggctgcag
     2881 cccggcgacg tcctcgtcgt ccagggcgac gaggacgccc tcgcgcgcct ctcggccgac
     2941 cgtggcgtcc tgatgctcgt tcccttccag ggccgcgccc gggtgctccg caagcgctgg
     3001 ctcgcgctcg ctatcctgct cggcgcaatc gccctcgcca tcgccggtgc cccgctgcag
     3061 atggcgatgc tcgtcggagc ggccgcgatg gtcctcaccc gttgcgtgac ccccggccag
     3121 gcctaccgtg ccatcgacca gcgcgtcttc gtcttcatcg cgggagcgat cccgctcggc
     3181 gccgcgatgg agaagaccgg cgcagcccgc acgatcgccg aactcctgga gcagccgctc
     3241 tctggcgcga cgcccttcat cgcgctcctc gcgatcttcg tcgccgtcgc cctcgtcacc
     3301 cagttcatgt cggacgccgc cacgaccgcg ctctttgccc ccatcgccct gggcctggcc
     3361 ggcgccatcg gcgtctcccc caccgcctgc gtcgtcacgg tcgccgttgg ctccgtcgcc
     3421 tcctcgatca ccccgctcgg tcaccacggc aacctgctcg tctatggccc cggcggctac
     3481 cgcttcagcg acttcgtccg cgccggcacg ccgctgaccc ttctggtcgg cgtcatcgcc
     3541 gccgccggca cccccctcct ctggccctga cccaccctca tatccgcgcc tgaccgcccg
     3601 acgagagggt gtacgatgcg ctccggccgc tcagacgtcg gctcgccgac gagccgcccg
     3661 aaggcgcgcg aagcgagaca cccgctatgg cccggtccgg cgacaccacc accgatctcg
     3721 tccaccgcgt gcccctgctc gcgagtctct ccgaggagga catccgctcc ctcgctccca
     3781 tcgccggccg ccgttcgttc cgtcacggcc acacgatctt ccgcgaagga gaaccggggg
     3841 attccctcta catcctgatc cgagggagtg tcttcatcac gatgctctcg agcgacgggg
     3901 cggagagcat cctcgcggtg ctacgcggcg gcgattgcct tggcgaactc tcgttactgg
     3961 acggcttccc ccgctcggct accgctcgcg ccgccgagga cgtcgacgct ctcgtcgtga
     4021 cccgcgacca cttccaatcg tggctcgccg atcaccacgc cgcctcgctc gcgctgcttc
     4081 ggacgctgag catgcgcatt cgccggacga acgccgcatt ggccgatcgc aacgcgctcg
     4141 acctctggca gcggctcgcc aagcagctcc tcctgctcgc cgatgaccag ccccgcacgt
     4201 cagcctccga cggccccgac ctccgcgtga cgcagcagca gctcgcggag atgctcggag
     4261 tcacccgcga ggccgtgaac aagacgctgc gcgaattcgc acaggccggc tggatcgaac
     4321 tccgacgagg gctcgtcagg atctgtgacg ccgcgagcct cgcggaacag ttgtagccgt
     4381 ccatcgcacc tgggcgggcg ccgtcgctag acaagcggaa tcaggaagct gcccctcgcc
     4441 tccgcggtgc ggcgcgccgc atcgagggcg cgtaggaact ggtcctgccg gatcgcccgc
     4501 tcgagcactc cagcgccgtc ggccgcgaaa cgagagtcgt gtgtggttgc agcgagttcc
     4561 gctcggcttc gggatgccgt cccactgccg gtcctgactt cgatcatgag tgcctccagc
     4621 cccgctggca gcccgaggag actgcccgtt ggctggagcg tgtatcgagc gtcgaaccgg
     4681 ttccatgaac tggcgcacag atcgccggac tcgcatcgat tcgtccctgc agcccccgcc
     4741 actccggtgc ggcgacaacc cccgggaggc cgtgctcaca cagcgatcat cgcagccagc
     4801 tcgtgaccga ggagctgcct caccagcgcc ctcaagtcgc cggcgggcgc ccagacctct
     4861 tcgatgtcca gaagcgcatt ggccagggct gctccctcgg gtgtcgcctg gtaccgccgc
     4921 cgccggatcg cctccgcgag ctctgcggcg gcgtccgccg acgcgggaag caggcgctcg
     4981 tcgatgccca gcagcgagcc ggcgacgttc cagctgtgcg cgtagtcata gagcgtccgc
     5041 gcgtcgacct ggacccccac ccgaggcaac cggatcgccg tcatccatga gagcgtgaga
     5101 agcgtggccg ccaggtcctc ctggttgagg cgcgcctccc ccgagggcga ttcggggcga
     5161 gtgcccggct ctccactcag gcgcagcgcg gcgtgtaaca gccgcactcg ctggatcgcg
     5221 cgacgcccgt ccgcaccgtc gcccaggccg cccgggctca tcaccgaact gacgaattcg
     5281 gcggtgcgcc gaactcggcg cagcggatcg acatcaagcg tcgagtgtcg gtttagttcc
     5341 cgtgccacgg ccgcgttgca gtacgactcc ggaagcgccc cgaagccgag caccgtcatc
     5401 accgccgccg actcccgttc gaacaacgcc tgccctcgtc cgactgcggc gacatccgtc
     5461 cagtcgggga ggcggcccgt cgcgacgagg taggaatcga tcgccgggtg cctcggaaac
     5521 cgctcgttga gttcggcaat gagttcgccg accacggccg gctcc
//
LOCUS       DAORPG010000043         5526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_859_length_5526_cov_3.530068, whole genome shotgun sequence.
ACCESSION   DAORPG010000043 DAORPG010000000
VERSION     DAORPG010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_859_length_5526_cov_3.530068"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /gene="murF"
                     /locus_tag="PLX85_01740"
     CDS             complement(<1..1133)
                     /gene="murF"
                     /locus_tag="PLX85_01740"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRC61930.1"
                     /translation="MSASPILDANFVAASLAGVITSHAGPNARFSRAVIDSREARQGD
                     LFVALPGEHADGHTYAADAVRRGASGALLARPLELGADAATFVVPDVLGALQRLAVAW
                     RNELSGLEVVGVTGNVGKSTTKLITAALLESRYRVQAETHNYNNEIGVPLCLLDLRPD
                     TERAVIEMGMYTTGEIAVLCTWARPRIGIVLNVGPVHLERAGSLDAIAQAKRELPEAL
                     PAEGHAILNIDDERVRAMAPFTRARVWTVGTAPDAAVRGDAIESHGAEGFEFTLHYRD
                     ASRRLHVPLAGAHLVTNVLAGVATALADGIGLDEVCEAVEDLDVPTRVVVRRSERGFT
                     VLDDTYNASPAATLAALELLSETPGRRIALLGDMLELGEVSAE"
     gene            complement(1130..2872)
                     /locus_tag="PLX85_01745"
     CDS             complement(1130..2872)
                     /locus_tag="PLX85_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRC61931.1"
                     /translation="MPESRPNHLTWRLLLLAGLVSIALVALVFRLATIQIVDHQQYAD
                     EAAATHAGVADLPAPRGAILDSSGFPLATSEDTWDVYIDRFLWRDAAKASAAANSLGV
                     ALGRDAQALLKLGTEGNNGDVVVQRQLPYEAGVQLRSQGLWGVRLLPSSKRVYPEGGL
                     ASSIVGFVGIEGTGLWGVESDFDGVLHGKPGLIGSERDALGRPISFGGRTERDPVAGG
                     DVTLTIDRFIQSIAEQRLKEAVSKYNARGGSIVVMDPNTGAILAMASVPSVAQSGINL
                     DDPKLLDEVRNRAITDLYEPGSVIKTLTTATAIDLGRVTPESTYVDTGEVRIGDATIK
                     NWDFSANGPTTVRQYLQKSLNTGSVWLSGLIGAKDFYRYLSAFGLGEPTHIGLSGEAD
                     GLIRQPSDDAWYPVDLATNSYGQGFAATPIQVLTAVNVFANGGRLMRPYIVSQVANNS
                     DVRHFDPVEVRRPVSAATAQTMARLMYDVVEGVDYHGARVPGYDVAGKTGTTLVSIPT
                     GYDLNSTIASFAGFIPYQQPRVSILVKIDQPSGGLNLGGQVAAPVFATVAADIMKYLR
                     VPATRPAELQVTAR"
     gene            complement(2907..3359)
                     /locus_tag="PLX85_01750"
     CDS             complement(2907..3359)
                     /locus_tag="PLX85_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61932.1"
                     /translation="MATLPQFRPQAGQQTTPRFGRPRHIPGTLLAGLALLILAGVGLL
                     QVLQTSRAATAGYELRSLENEQQTLSAEVRLLEAQIAQQTRVEQIRRTAVDRLGMVPP
                     QQTVHIAVGVTAPSSMPLPERYVVQPQHVETPPLSIWERFLRRIPGFN"
     gene            complement(3359..4318)
                     /gene="rsmH"
                     /locus_tag="PLX85_01755"
     CDS             complement(3359..4318)
                     /gene="rsmH"
                     /locus_tag="PLX85_01755"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRC61933.1"
                     /translation="MSHPPYEPRHEPVLLHETLAALDVRPGGSYIDATLGLGGHAEAI
                     LEAAQPDGRLLGIDRDPAAIEVAGDRLARFGEDAVLVEGNYEQIADIAEDQGFSGVDG
                     VLFDLGVSSMQLDAPGRGFSFQRDEPLDMRMGPDARLSAADIVNTYDEAELADLIWKY
                     GEERRSRAIARSIVARRPIDTTGALVNAIEQAVGRGRERQIHPATLTFQALRIAVNGE
                     LSSLEDALEGARGLLAGVGSRLVVISFHSLEDRIVKRYMIKESSTCVCPPRTPVCICG
                     KTSRLRLSSKRAVQASEGEIARNPRARSARLRVAESVVERRAA"
     gene            complement(4315..4758)
                     /locus_tag="PLX85_01760"
     CDS             complement(4315..4758)
                     /locus_tag="PLX85_01760"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRC61934.1"
                     /translation="MFFYGTYEHAIDERGRIAIPSGYRPQFGGGGVVRVGAEGCVELY
                     TQEGFEAEAERRLSSEESTRELSARRTRRSFLAGAFPVELDKQGRILLPQPIRSMAHL
                     NGRASIVGCGDYMEIWERDSWNAEQAAFSAAEAGSTVGAGEGTDQ"
     gene            4901..5392
                     /locus_tag="PLX85_01765"
     CDS             4901..5392
                     /locus_tag="PLX85_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HRC61935.1"
                     /translation="MDEVVRAAVLTISDAGSRGERVDTAGPALVRMLDDAGFVVAETA
                     ICPDEPAEIAALLRRWSDAEAYALILTTGGTGLSPRDRTPEATAEVLDYRVDGMAEAM
                     RAAGLASTPRAMLSRGLAGVRGRTLIVNLPGSERGATENLTTVLPVLRHAADLLRGDT
                     AHA"
BASE COUNT          877 a         1945 c         1775 g          929 t
ORIGIN      
        1 tcttccgccg atacctcacc cagttccaac atgtcgccga gcagcgcgat ccgccgcccg
       61 ggcgtctccg acagcaactc cagcgctgcc agggtcgctg ccgggctcgc gttgtaggtg
      121 tcgtcgagga ccgtgaatcc gcgctcgctc cggcgcacca ccacacgcgt cggcacgtcg
      181 aggtcctcca cggcttcgca cacctcgtcc agtccgatcc cgtcggcgag ggcggtggct
      241 actccagcaa ggacattggt cacgaggtgc gcgccggcga gcgggacatg caaccgccgc
      301 gaggcgtcgc ggtagtgcag ggtgaactcg aaaccctcgg cgccatgact ctcgatcgcg
      361 tcgccgcgca ctgcggcgtc tggcgccgtg ccgacggtcc acacgcgcgc cctcgtaaac
      421 ggggccatgg cacgcacgcg ctcgtcgtcg atgttgagga tggcgtggcc ttcggccggc
      481 agagcctcag gcagctcccg cttcgcttgg gcgatcgcat cgagcgaccc cgcgcgttcg
      541 aggtgcaccg ggcccacgtt gagcacgatc ccgatgcggg gccgcgccca cgtgcagagc
      601 accgcgatct ctcccgtcgt gtacatgccc atttcgatca cggcgcgttc ggtatcgggc
      661 cgcaggtcaa gcaggcacag cggcacgcca atctcattgt tgtagttgtg cgtttccgcc
      721 tgcacgcgat accggctttc cagcagggcg gcggtgatca gcttcgtcgt cgacttccct
      781 acattcccgg tcacgccgac cacttcgagg cccgagagct cgtttcgcca ggcgacggca
      841 agccgttgca gcgcgccgag cacgtccggg accacgaagg tcgcggcgtc ggcgccgagc
      901 tcaagcggcc gagcgagcag cgcgccgctg gcgccacgcc gcacggcgtc cgcggcgtac
      961 gtgtgtccgt cggcatgctc cccgggcaga gcgacgaaca ggtcgccctg cctggcctcg
     1021 cgcgaatcga tcactgctcg gctgaatcgc gcgtttggcc ccgcatgact ggtgatgacg
     1081 ccggcgaggg aggcggccac gaaattggcg tcgaggatgg gcgaagcgct catcgcgcag
     1141 tcacctggag ttcggccggg cgcgtggccg ggacccgtag gtacttcatg atgtcagctg
     1201 cgacggtcgc aaataccgga gccgccacct ggccgccgag gttcaaccca ccgctcggct
     1261 ggtcgatctt cacgaggatg gacaccctcg gctgctggta cgggatgaag cccgcgaacg
     1321 aggcgatcgt gctgttgaga tcgtaccccg tcgggatgga cacgagtgtc gtcccggtct
     1381 tgcccgccac gtcgtatccc ggcacccgcg ccccgtggta gtcgacgccc tcgaccacgt
     1441 cgtacatgag acgcgccatc gtttgagcgg tggccgccga gacgggccgt cgtacctcga
     1501 ccgggtcgaa atggcgcacg tcactgttgt tggccacctg cgacacgatg tacggccgca
     1561 tcaggcggcc accgttcgcg aacacgttca cggcggtcag gacctggatc ggcgtcgcgg
     1621 cgaacccctg tccgtaggag ttcgttgcga ggtccacggg ataccaggca tcgtcgcttg
     1681 gctgccggat cagcccatcc gcctctcccg agagtccgat gtgggtcggt tcgcccagtc
     1741 cgaacgcgct caggtagcgg tagaagtcct tcgctccgat aagtccggac agccagacag
     1801 agccggtgtt cagcgacttc tgcaggtatt ggcgcaccgt cgtcggcccg ttggccgaga
     1861 agtcccagtt cttgatcgtg gcgtcgccaa tgcgcacctc acccgtgtcg acgtatgtgg
     1921 actcgggagt cacccggccg agatcgatcg cggtcgcggt ggtcagcgtc ttgatcaccg
     1981 aacccggctc gtagaggtcc gtgatcgccc ggttgcgcac ctcgtcgagc aacttcgggt
     2041 catcgaggtt gatcccagat tgagcgacag acggcaccga cgccatggcg aggatggctc
     2101 cagtgttcgg gtccatcact acgatgctgc ccccgcgcgc gttgtacttc gagaccgctt
     2161 ccttgagccg ttgctcggcg atcgactgga tgaagcggtc gatggtcagc gttacatcgc
     2221 caccggccac cgggtcgcgc tccgtgcgcc caccgaacga gattggccgc ccaagagcgt
     2281 cgcgctccga cccgatcagc ccgggctttc catgcaacac cccgtcgaag tcggactcca
     2341 ccccccacag gcccgtgcct tcgatcccga cgaatcccac gatggagctg gcgaggccgc
     2401 cttctggata tacccgcttc gaggagggga gcagacgaac gccccagagg ccctgcgatc
     2461 gcagttgcac accagcctca taggggagct gccgttggac gacgacgtcg ccattgttcc
     2521 cttcggtccc gagcttcaat agcgcctgcg catcgcgtcc cagcgccaca ccaaggctgt
     2581 tggcggcggc ggaagccttc gccgcgtccc gccataggaa gcggtcgatg tagacgtccc
     2641 acgtgtcctc cgaggtggcg agtgggaacc cggatgaatc gaggatcgca ccccggggcg
     2701 ccgggaggtc tgcaaccccc gcgtgtgtgg ccgccgcctc gtccgcgtac tgctggtggt
     2761 cgacgatctg gatcgtggca agccggaaca cgagtgcgac cagcgcgatg ctgaccagcc
     2821 cggcgaggag gagcaggcgc caggtcaggt ggttaggcct cgactcgggc aagcgggtct
     2881 tctcctcgcc tccggccgcg cggccgctag ttgaaccccg gaatcctccg taggaagcgt
     2941 tcccagatcg acagcggcgg cgtctcgacg tgctgtggtt gcacgacgta gcgctccggc
     3001 aacggcatcg aggacggtgc tgtgacaccg accgcgatat gcaccgtctg ctgtggcggg
     3061 accatcccca gtcggtcgac tgcggttcgg cggatctgct cgacacgcgt ctgttgggcg
     3121 atctgcgcct ccagcaggcg gacctccgcg ctcagcgtct gctgctcatt ctcgagcgat
     3181 cgcaactcgt agccggcggt cgccgcccgg ctcgtctgta gcacctgcaa cagccccacg
     3241 cccgcgagga tcagcagtgc cagcccggcc agcagtgtgc ccgggatgtg gcgcggccga
     3301 ccgaagcgag gcgtggtctg ttgcccggcc tgcggacgga actgcggcag cgtcgccatc
     3361 aggcggccct ccgctcgacg actgactcag ccacacgaag gcgagcagaa cgggcgcgcg
     3421 gattacgcgc gatctcgccc tcgctcgcct gcacggcccg cttgctgctc agacgcaatc
     3481 tcgatgtctt tccacatata cacactggcg tgcgcggtgg acatacgcaa gtgcttgact
     3541 ccttaatcat atatcgtttc acaatacgat cctcgagcga gtggaacgaa atcaccacca
     3601 ggcgggagcc aacgccagcg agcaggccgc gggcgccttc aagcgcgtcc tcgagcgagc
     3661 tgagctcgcc gttgacggct atccgcaggg cctggaacgt cagagtggcg gggtggattt
     3721 gacgttccct gccccgtccc acggcctgct cgattgcgtt gaccaatgct ccggtcgtgt
     3781 cgatcgggcg gcgagccacg atggacctcg cgatcgcccg gctgcggcgc tcctctccgt
     3841 atttccagat caggtcggcg agctcggcct cgtcgtaggt atttacgatg tccgccgcgg
     3901 acaggcgcgc atccgggccc atccgcatgt ccagcggctc atcccgctgg aatgagaagc
     3961 ctcgtccggg cgcgtccagc tgcatcgacg acacgccgag gtcaaacagc acgccgtcaa
     4021 ccccgctgaa cccctggtct tccgcgatgt cggcgatctg ctcgtagttc ccttcgacga
     4081 gcaccgcgtc ctcgccaaac cgcgccagtc ggtcgccagc cacctcgatc gccgccgggt
     4141 cacgatcgat gccaagcagc cggccgtccg gctgcgcggc ctcgaggatc gcctctgcgt
     4201 ggcccccgag acccagtgtg gcatcgatgt agctcccacc gggccggaca tcgagagcgg
     4261 cgagcgtttc gtggagcaag accggctcgt gcctgggctc gtacggggga tggctcattg
     4321 gtccgtccct tcgcctgcgc ccacggtcga tccggcctcg gcagcgctga acgcggcctg
     4381 ttccgcgttc cagctgtcgc gctcccagat ctccatatag tcgccacagc ccacgatcga
     4441 cgctcgcccg ttcagatgcg ccatcgaccg aatcggctgc ggcagcagga tgcggccttg
     4501 cttgtccagc tccaccggga acgcccccgc gaggaagctg cgtcgcgttc gcctggcact
     4561 gagctcgcga gtgctctctt cgctgctgag acggcgctcc gcctccgcct cgaatccttc
     4621 ttgcgtgtac agctcgacgc agccctcggc ccccacgcgc acgactccac cgccgccaaa
     4681 ttgcggacgg tagcccgagg ggatggcaat ccgcccgcgc tcgtcgatcg cgtgctcgta
     4741 ggtaccgtag aagaacaccc acgccgcctt gctgcagggc ggggcggaga cagattcccc
     4801 acttttcccc acctgcatcc cgaggctacc acaacgcccc agatcgcggc aatgcttcgc
     4861 ctcgaactgc tgctaacatc gacttgagat gggagacgct atggacgagg tcgtgcgcgc
     4921 ggccgtgctc accatcagcg acgccggctc acgaggcgag cgagtcgata cggctggccc
     4981 cgcgctggtc cgaatgctcg atgacgccgg ctttgtggtc gctgagaccg ccatctgtcc
     5041 ggacgagccg gccgagatcg ccgcgctcct gcgccgttgg tccgatgccg aggcgtacgc
     5101 gctcatcctc accaccggag gcaccggcct gagcccgcgc gaccgcacgc cagaggcgac
     5161 cgccgaggtg ctcgactacc gggtcgatgg catggcagag gcaatgcgag cggccgggct
     5221 cgcctcgaca ccccgtgcga tgctttcccg cggactcgcg ggcgtccgtg gtcggacgct
     5281 tatcgtgaac ctgcccggaa gcgaacgcgg cgccaccgag aacctgacca cggtcttacc
     5341 cgtgctccgt cacgccgccg acctgctccg aggtgatacc gcgcacgcct agggacgacg
     5401 cgtcggccca ctcggtcagc gccgccgtta gcgtccctgg aagtcgggtt cgcgccgttc
     5461 gaggaacgag ttcacgccct cgacgccgtc ttccgagcgg aagaggtgct gcacgaactg
     5521 ccccgt
//
LOCUS       DAORPG010000044         5484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_872_length_5484_cov_3.813962, whole genome shotgun sequence.
ACCESSION   DAORPG010000044 DAORPG010000000
VERSION     DAORPG010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_872_length_5484_cov_3.813962"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PLX85_01770"
     CDS             <1..592
                     /locus_tag="PLX85_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRC61936.1"
                     /translation="GLEGFRGLPHRLQRVREASKRTGEAPPVIGKQRVRDGRTGEYFD
                     QPVTVGYIYMLKLVHLVEDKIHARSTGPYSLITQQPLGGKAQFGGQRFGEMEVWALEA
                     YGAAHILQEMLTVKSDDVVGRVKAYEAIVKGENTMEPGVPESFKVLVKELQSLGLSVE
                     ILNEDEEQVAFSEDYIEAIPSLGINLSGREAEDVRA"
     gene            579..4946
                     /gene="rpoC"
                     /locus_tag="PLX85_01775"
     CDS             579..4946
                     /gene="rpoC"
                     /locus_tag="PLX85_01775"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRC61937.1"
                     /translation="MYVLEVNDFNAIRLSLASPAQIRSWSYGEVTKPETINYRTLKPE
                     KDGLFCEKIFGPTKDFECYCGKYKRVRYKGIVCDKCGVEVARSKVRRERMGHVSLAAP
                     VAHIWFSKGVPSRLGLLLDIPPRTLERVLYFAQYVITEVNEEARAHALELLQQEIDEE
                     VSRRQGETSSRIAVREESLDQEIAEISRRRDAALEEADQELQRDIDTVMSEARALDED
                     LQGRHGEKLRARLTFRDQTLAQRGDEIGSETSAALHELAREQVASVEERIANKKADHQ
                     LMADAAIQQKRDQAFQELEPLRQQVSDVRTQVRKEYDPLVKQLEKLRDPIAADQLVVL
                     TEQEHRDLEDRFGLVFRSGMGAESILSILQRLDLDALRQRLQEEVQSTSGQRRKKATK
                     RLRVVESLRKSGNKPEWMIIQELPVLPPDLRPMVQLDGGRFATSDLNDLYRRVINRNN
                     RLKRLLNLGAPEIIIRNEKRMLQEAVDSLIDNGRRGRAVSGSGNHKLKSLSDLLKGKQ
                     GRFRQNLLGKRVDYSGRSVIVVGPDLRLHQCGLPKKMALELFKPFIMHALVRNGLAHN
                     IKSAKRIVERARPEVWDVLEEVIRDRPVLLNRAPTLHRLGIQAFEPVLVEGSAIQLHP
                     LVCFAYNADFDGDQMAVHVPLSYEAVAEARQVMLSTRNLLSPSDGEPVVAPTLDMVLG
                     CYYMTYDSPRNESQQRRAFGSVADVRMAYDLGYSRIHDRIRLRMDGQLVETTLGRVLF
                     NEIVPAGIGFQNALMNKKALRELVSHIHTTFGPEATVEFVDAMKDIGFRYATQSGISI
                     AVDDIRVPSTKPALMAEADQRVDDMEEQYQLGLVTENEKYQAAIDIWTHTTNRIQEAL
                     EQTLDPEGPLSMMSTSGAKGNIAQIRQMAGMRGLMTAPSGRIIDLPIRSSFREGLSVL
                     EYFISTHGARKGLADTALRTADSGYLTRRMIDVAQDLIVLVEDCTAPDEAIPGIWLRE
                     RAEAGILASLQERVTGRWAASPVADPQTGEIIVEANHEIREEAAQRIIDSQVEQVHVR
                     SPLTCQAKRGVCAMCYGRSLATGQLVERGVAVGIIAAQSIGEPGTQLTMRTFHTGGVA
                     SAADITSGLPRVEEIFEARVPRGRATISEIDGIVQVIRSGDTRMIAVSNTESFAEEYE
                     LPDELELLVDEGDMVDTAQVLAQPGEGADGSLPMVPVKAAIAGRVEFVRGNPRRRRPT
                     ALRIVSEETDQREYQIEASARVRVESGEYVRAGQQLTEGALDPQDVLSILGPEAVQIY
                     LVDEVQRVYRSQGVNINDRHIEVIVRQMLRKVRVEEAGDTDLLPSELLDRHAYEDMNA
                     RLVAEGGEPATAVPVLLGVTRASLSTDSFLAAASFQETTRVLTDAAISGATDHLRGLK
                     ENVIIGKLIPARAPITVERPTPIAEISMPESLLLPFIFDFERPEPLLPGDEESLFELL
                     QQGPGALAGAGAGYVAEPD"
     gene            complement(5038..5217)
                     /locus_tag="PLX85_01780"
     CDS             complement(5038..5217)
                     /locus_tag="PLX85_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61938.1"
                     /translation="MVLLVLWGLFLALVAYSFVFWRSGWPGVLIMTVVLAVTLPTNAW
                     WIRRRVRQIRSSPDG"
BASE COUNT          937 a         1671 c         2079 g          797 t
ORIGIN      
        1 cgggctcgag ggcttccgcg gcttgcccca tcggctgcag cgggtgcgcg aggccagcaa
       61 gcgcacgggc gaagcgccgc cggtgatcgg caagcagcgc gtgcgtgacg ggcggacggg
      121 cgagtacttc gaccagccgg tgaccgtggg ctacatctac atgctcaagc tggtccacct
      181 ggtggaggac aagatccacg cccgctcgac gggcccttac tcgctgatta cgcagcagcc
      241 cctgggcggc aaggcgcagt tcggcggcca gcgcttcggc gagatggagg tgtgggcgct
      301 cgaggcgtac ggcgcggcgc acatcctcca ggagatgctc acggtgaagt cggacgacgt
      361 ggtcggacgc gtgaaggcgt acgaggcgat cgtcaagggc gagaacacca tggagcccgg
      421 cgtgccggag tcgttcaagg tgctcgtcaa ggagctgcag tcgctcggcc tgagcgtcga
      481 gattctgaac gaggacgagg agcaggtagc cttctcggag gactacatcg aggcgatccc
      541 gtcgctcggc atcaacctgt caggacgcga agcggaggat gtacgtgctt gaggtcaacg
      601 atttcaatgc cattcgcctg tcgcttgcct cgccggcaca gatccgatcg tggtcgtacg
      661 gcgaagtcac gaagccggag acgatcaact accgcacgct gaagccggag aaggacgggc
      721 tcttctgcga gaagatcttc ggtccgacga aggacttcga gtgctactgc gggaagtaca
      781 agcgggtccg ctacaaggga atcgtgtgcg acaagtgcgg cgttgaagtc gcacggagca
      841 aggtgcgccg cgagcggatg gggcacgtga gtctcgccgc gccggtggcg cacatctggt
      901 tctcgaaggg cgtgccgagc cgcctcggtc tgctgctgga cattccgccg cggacgctgg
      961 agcgcgtgct gtacttcgcg cagtacgtga tcacggaagt gaacgaggag gcgcgggcgc
     1021 acgcgctcga gctgctgcag caggagatcg acgaggaagt cagccggcgg cagggagaga
     1081 cctcgagccg gatcgccgtg cgcgaggagt cgctcgatca ggagatcgcg gagatctcgc
     1141 gacggcggga cgcggccctc gaagaggcgg accaggagct gcagcgcgac atcgacaccg
     1201 tgatgtccga ggcgcgggcg ctggacgagg acctgcaggg acgccacggc gagaagctgc
     1261 gggcgcgcct gacgttccgc gaccagacgc tggcgcagcg cggcgacgag atcgggagtg
     1321 agacctcggc ggcgctgcac gagctggctc gcgagcaggt ggcgagcgtc gaagagcgca
     1381 tcgcgaacaa gaaggccgac caccagctga tggcggacgc cgccatccag cagaagcgcg
     1441 accaggcgtt ccaggagctg gagccgctgc ggcagcaggt gtcggacgtg cgcacgcagg
     1501 tgcgcaagga gtacgacccg ctggtgaagc agctggagaa gctgcgcgac ccgatcgcgg
     1561 ccgaccagct ggtggtgctg accgagcagg agcaccgtga cctcgaggac cgcttcggcc
     1621 tcgtgttccg gtcggggatg ggcgcggagt cgatcctctc gatcctgcag cgactggacc
     1681 tcgacgcgct gcgtcagcgg ctgcaggaag aggtgcagtc gacctcgggg cagcggcgca
     1741 agaaggcgac gaagcggctg cgcgtcgtgg agtcgctgcg gaagtcgggc aacaagccgg
     1801 agtggatgat catccaggag ctgccggtgc tgccgccgga cctgcgcccg atggtgcagc
     1861 tcgacggtgg ccggttcgcg acctcggacc tgaacgacct gtaccggcgc gtgatcaacc
     1921 gcaacaaccg gttgaagcgc ctgctgaacc tcggcgcgcc ggagatcatc atccgcaacg
     1981 agaagcggat gctgcaggag gcagtcgact cgctgatcga caacgggcgg cgtggccgcg
     2041 cggtgagcgg gtcgggcaac cacaagctga agtcgctgtc tgacctgttg aagggcaagc
     2101 aggggcggtt ccgccagaac ctgctcggca agcgcgtcga ctactcgggg cgctccgtga
     2161 tcgtggtggg gccggacctg cggctgcacc agtgcgggct gccgaagaag atggcgctcg
     2221 agctgttcaa gccgttcatc atgcacgccc tcgtgcggaa cgggctggcg cacaacatta
     2281 agagtgcaaa gcggatcgtg gagcgggcgc gtcccgaggt gtgggacgtc ctcgaagagg
     2341 tgatccgcga ccggccggtg ctcctgaacc gtgcgccgac gctgcaccgc ctcggcatcc
     2401 aggccttcga gccggtgctg gtcgaggggt cagcgattca gctgcacccg ctcgtctgct
     2461 tcgcgtacaa cgcggacttc gacggtgacc agatggccgt gcacgtgccg ctgtcctacg
     2521 aggcggtggc cgaggcgcga caggtgatgc tgtcgacgcg caacctgctg tcgccctcgg
     2581 acggcgagcc ggtggtggcg ccgacgctgg acatggtgct cggctgctac tacatgacgt
     2641 acgactcgcc tcggaacgag tcgcagcagc gtcgcgcgtt cggctcggtg gcggacgtgc
     2701 gcatggccta cgacctgggc tactcacgca tccacgaccg catccgcctg cggatggacg
     2761 gtcaactggt ggagacgacg ctggggcgcg tcctgttcaa cgagatcgtc ccggcgggca
     2821 tcgggttcca gaacgcgctg atgaacaaga aggcgctgcg cgagctcgtg tcgcacatcc
     2881 acacgacgtt cgggccggaa gcgacggtcg aattcgtgga cgcgatgaag gacatcgggt
     2941 tccggtacgc gacgcagtcg ggcatctcga tcgcggtgga cgacatccgc gtgccctcga
     3001 cgaagcccgc gctgatggcg gaagcggacc agcgcgtcga cgacatggag gagcagtacc
     3061 agctcggcct cgtgacggag aacgagaagt accaggccgc catcgacatc tggacgcaca
     3121 cgacgaaccg tatccaggag gcgttggagc agacgctcga cccggagggt ccgctgtcga
     3181 tgatgtcgac gtcgggcgcg aaggggaaca tcgcgcagat ccgccagatg gcgggtatgc
     3241 gcggcctgat gaccgccccc tcggggcgga tcatcgacct gccgatccgc tcctcgttcc
     3301 gcgaggggct gtcggtgctc gagtacttca tctcgacgca cggcgcgcgc aagggcctcg
     3361 cggacacggc gctgcgcacg gcggactcgg gctacctgac gcggcgcatg atcgacgtgg
     3421 cgcaggacct gatcgtcctc gtcgaggact gcaccgcacc ggacgaggcg atcccgggca
     3481 tctggctgcg tgagcgggcg gaggcgggca tcctcgcgtc gctgcaggag cgcgtgacgg
     3541 gccgctgggc ggcctcgccg gtcgcggacc cgcagacggg cgagatcatc gtcgaggcga
     3601 accacgagat ccgcgaagag gcggcgcagc gcatcatcga ctcgcaggtg gagcaggtgc
     3661 acgtgcggtc gccgctgacg tgccaggcga agcgcggcgt gtgcgcgatg tgctacgggc
     3721 ggtcgctggc gacgggccag ctcgtcgagc gcggcgtggc ggtgggcatc atcgcggcgc
     3781 agtcgatcgg tgagccgggc acgcagctga cgatgcgtac gttccacacg ggtggtgtcg
     3841 cctcggcggc ggacatcacc tcgggtctgc cgcgcgtgga ggagatcttc gaggcgcgcg
     3901 tgccccgcgg gcgcgcgacg atctcggaga tcgacggcat cgtgcaggtc atccgctcgg
     3961 gtgacacgcg catgatcgcg gtctcgaaca cggagtcgtt cgcggaggag tacgagctgc
     4021 cggatgagct cgagctgctc gtggacgagg gcgacatggt ggacaccgca caggtgctcg
     4081 cccagcccgg cgagggcgcg gacggctcgc tgccgatggt gcccgtgaag gcggcgatcg
     4141 cgggccgcgt ggagttcgtg cgtgggaacc cgcggcgacg ccggccgacg gcgctgcgga
     4201 tcgtctcgga agagacggac cagcgggagt accagattga ggcgtcggcg cgggtgcgcg
     4261 tcgagtctgg cgagtacgta cgggcggggc agcagctcac cgaaggtgcg ctggacccgc
     4321 aggacgtgct gtcgatcctc ggtccggagg cggtgcagat ttacctcgtt gacgaggtgc
     4381 agcgtgtgta tcgctcacag ggcgtgaaca tcaacgaccg tcacatcgag gtgatcgtgc
     4441 ggcagatgct gcggaaggtg cgcgtggagg aggcgggcga cacggacctg ctgccctccg
     4501 agctgctgga ccggcacgcg tacgaggaca tgaacgcgcg cctggtggcg gagggcggcg
     4561 agccggcgac ggcggtgccg gtgctcctcg gcgtgacgcg cgcctcgttg agcacggact
     4621 ccttcctcgc ggcggcgtcg ttccaggaga cgacccgggt cctgacggac gcggcgatct
     4681 cgggggcgac ggaccacctg cgggggctga aggagaacgt gatcatcggc aagctgatcc
     4741 cggcacgagc gccgatcacg gtcgagcggc cgacgccgat cgcggagatc tcgatgccgg
     4801 agtcgctgct gctgccgttc atcttcgact tcgagcggcc ggagccgttg ctgccgggcg
     4861 acgaggagtc gttgttcgag ctgttgcagc aggggccggg cgcgctggcc ggcgcgggcg
     4921 cggggtacgt ggccgagccc gactagcgcc tcgacaggtc cgagtgcagt gcgaagcccc
     4981 ggcgatgagc cggggcttcg ttgtggggtt ggtggggttg ggtcggtggg ccgggcgtca
     5041 gccgtccggt gacgatcgga tctgtcgcac tcgtctccgg atccaccagg cgttcgtcgg
     5101 gagcgttacg gcgaggacga ctgtcataat cagcaccccg ggccaccctg agcgccagaa
     5161 gacaaaggag tacgcgacca gcgccaggaa caggccccag aggacgagga gaaccaccgc
     5221 ccgtctgctg aggcgtcgcg ggggtcgctc tcgataccgg tccacaacgg cagtctggcg
     5281 acgccgggcg gatatcgcaa gatgccattg cggcgcttcc ggtcattcct cggcggctgg
     5341 tgtatcgtga cgctcgcacc aagggaggcg tcgatggagt ggcgtgacga cgaggagcag
     5401 gcggcgttcc ggagcgaggt cgcgcggttc atcgaggcga acctgccgcc gctgtaccgg
     5461 caggaggcgg gcgacgcgga cgcc
//
LOCUS       DAORPG010000045         5439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_888_length_5439_cov_2.852155, whole genome shotgun sequence.
ACCESSION   DAORPG010000045 DAORPG010000000
VERSION     DAORPG010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_888_length_5439_cov_2.852155"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="PLX85_01785"
     CDS             complement(<1..801)
                     /locus_tag="PLX85_01785"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRC61939.1"
                     /translation="MNASWTRRRTTWPWAIPALALLGPFLLWPLGAMVWRAVTPDGSF
                     SLGPLGDVVGDGYYWKRLGFTAGQAAASTALSVLVGLPAAYVFARLRFPLRGFLLALL
                     TVPFVLPTLVVAIAFQQLVGPGGWLNDVLEAMGLDPIRPLGTLGIILAAHVFYNVSIV
                     IRLVAAVWRNLDPRTEEAARLLGAGPLAAFRAVTLPALTPAILSAAALVFTFTFTSFG
                     VVLILGAGNPDLDTLEVVVYRLATRLVDLPAAALVSTIQIGATITALTL"
     gene            complement(798..1844)
                     /locus_tag="PLX85_01790"
     CDS             complement(798..1844)
                     /locus_tag="PLX85_01790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HRC61940.1"
                     /translation="MRTIRTLIILVCFASVGLLVLSCGDDDGGQPTLVVLTHSSFDID
                     RELVARFEQEHHVTVDIRKGGDANEVVNRALLNAGNPEGDVLYGIDNLTLPRTVGKGL
                     FEEYTSTLRDRIPSDVRAGLGDSREVTPIDYGYVALNFDRAKGTAPSRLEDLTNAAWR
                     GKLVVEDPTTSSPGLQFLATTVAQFGETGSYTWKDYWRDLRANDVLVVDGWDAAYNTY
                     FSRNGGDRPVVVSYTTSPAAEVFFSEGKLSEPPTSNVIPAGTKLFRQVEAAGVLRGTK
                     QPELAGQFIDFLLSDDVQAHIPETMFVYPVMPGLATPDWWQWAKVDVQPAALSVDGPT
                     IERWVREWTDIMRR"
     regulatory      complement(1964..2064)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(2108..3700)
                     /locus_tag="PLX85_01795"
     CDS             complement(2108..3700)
                     /locus_tag="PLX85_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC61941.1"
                     /translation="MSDDTLPPYVRGLLEPSAYPEPPASVELAQTHISYVFLAGDVVY
                     KTKKPVDFGFISQLTPEAREQYCHDEVRLNSRLAPDVYLGVVPVVRRADGRFAVESEG
                     EVVEWAVKMRRLPDDRTLDRILAGEGPPPRLAERLVQTLIAFHEAAAVVPNDPEYAGG
                     PGERKWWSREYGEAAGFIGDTWRAEDAEATKAFFDETIEAQQALFDQRLADGRVVEGH
                     GDLHAKHVYLLEGGEQVAIVDCIEFTDWFHFRYLDVGYDIAFLAMDLEARGFPELGDE
                     VAGRYIAAACDETMGVLQPLHRAFRAFVRGKVESIGAHATEISTEERERLWASAAAYF
                     RLAASFGGRRAAPCVVVMSGLPATGKSTVAATLAGRIGAAYLSSDATRKQLAGLNPRE
                     RAAAEFRSGLYNAEMTERTYEELRTRAHVHLEAGRPVVIDAMHGRASERAAAIEMARA
                     HGVPALIVELRLDDEAARARIEGREDDPLRTSDATWEVYERQRGQFEAVGTSEGPHLV
                     LDATLPPAASARAIAERLPARS"
     gene            complement(3755..5326)
                     /locus_tag="PLX85_01800"
     CDS             complement(3755..5326)
                     /locus_tag="PLX85_01800"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRC61942.1"
                     /translation="MPEDIAFRPAAEGGDASIVPVRRRPVGPRTDRVLYAGIKQYVTR
                     SAESEPGVEAEEHEGPPEPPPTTADETGEGVDEEATSKRRRRGRRGGRRHRHAEGVEG
                     EAASEADSDEAEDASEDDEEPTYEDAVAVQAEERERHAPPPIEVDPASFPPAFVALGL
                     NERSLAAVTNFGFTTPSPIQEQAIPLLLSGRDVVGIAQTGTGKTLAFGLPMVERLDAG
                     HRAVQGLVLVPTRELAQQVLEVLQDLGRYYGLEAVGLLGGRSLDRDFRALERQPQIVV
                     GTPGRVIDHLHRQTLSLNAVRYAVLDEADQMLDIGFLPDITRILTRTPRARQTALFSA
                     TMPHSILRLVRRYMEEPETVTIAPEQTTVETVEQVYFEVAQRDKIRGLRELIERELRG
                     RTLVFSRTRRGVDYLRDQLSDAGIRVGALHGDMDQRKRDHVVQQFRSGQLDILVATNV
                     AARGLDIPEITHVLNYDVPQNPEEYVHRIGRTGRAGRTGKAITFVCERDVAEFDTLIA
                     AFGDRLQKEHLELYA"
BASE COUNT          814 a         2084 c         1722 g          819 t
ORIGIN      
        1 cagcgtgagc gccgtaatcg tcgccccgat ctggatcgtg gacaccagcg ccgcggccgg
       61 caggtccacc aggcgggtcg ccagccggta gaccaccacc tcgagcgtat cgaggtcagg
      121 gttccccgct ccgaggatga ggaccacccc gaacgacgtg aacgtgaagg tgaacacgag
      181 cgccgccgcc gagaggatcg cgggcgtcaa cgcgggcagc gtcaccgcgc ggaacgccgc
      241 cagtggcccc gcacccagca gccgggccgc ctcctccgtc cgcggatcga ggttgcgcca
      301 caccgccgcg accagccgga tcacgatcga gacgttatag aacacgtggg cggccaggat
      361 gatgccgagc gtgccgagcg gccgtatcgg atcgagcccc atcgcctcga ggacgtcgtt
      421 gagccagccg cccggaccga ccagctgctg gaacgcgatc gccacgacca gcgtcggcag
      481 cacgaacggc acggttagca acgcaagcag gaacccccgc agcgggaaac gcaaccgggc
      541 gaacacatac gctgccggca gccctacgag cacagacagc gccgtcgagg cggcagcctg
      601 gccggccgtg aatcccagcc gtttccagta gtacccgtcc cccaccacgt cgccgagcgg
      661 cccgagagag aagctgccgt caggcgtgac cgcccgccac accatcgctc ccagcggcca
      721 cagcaggaac ggcccgagca gcgccagcgc cgggatcgcc cacggccacg tcgtccgccg
      781 ccgcgtccag ctcgcgttca tcggcgcatg atgtccgtcc actcacgcac ccagcgctcg
      841 atcgtcggcc catcgacgct gagcgccgcg ggttgcacgt cgaccttcgc ccactgccac
      901 cagtcaggcg tcgcaaggcc gggcatcacg gggtacacga acatcgtctc cggaatgtgc
      961 gcctgcacgt cgtccgagag caagaagtcg atgaactgac ctgccagctc gggctgcttc
     1021 gtgccacgca gcacgcccgc cgcctcaacc tgccggaaca gcttcgtccc tgccgggatc
     1081 acgttcgagg tcggcggctc gctcagcttc ccttcgctga agaacacctc cgcagccggg
     1141 cttgtcgtgt acgacacgac caccggtcgg tcgccgccgt tgcggctgaa gtacgtgttg
     1201 tacgccgcgt cccagccgtc gaccacgagc acgtcgttcg cgcgcaggtc gcgccagtag
     1261 tccttccacg tgtacgaccc ggtctcaccg aactgcgcca ccgttgtcgc gaggaactgc
     1321 aggcccggcg acgaggtcgt cgggtcctcg accacgagct ttccacgcca cgccgcgttc
     1381 gtgaggtcct ccagacggct cggtgccgtc cccttcgccc gatcgaagtt cagcgcgacg
     1441 tacccgtagt cgatcggcgt cacctcccgc gaatccccga gccccgcccg gacgtccgag
     1501 gggatgcggt cgcgcagcgt cgaggtgtac tcctcaaaga gccccttccc gaccgttcgc
     1561 ggcagggtca ggttgtcgat cccgtacagc acgtcgccct cggggttccc cgcgttgagc
     1621 agtgcgcggt tcacgacctc gttggcgtcg ccgcccttgc ggatgtcgac ggtaacgtgg
     1681 tgctcctgct cgaaccgcgc gaccagctcg cggtcgatgt cgaacgagct gtgggtcaac
     1741 accaccagcg tgggttgccc gccgtcgtcg tctccgcagc tgagcaccag cagcccgacg
     1801 ctcgcgaagc agacgagaat gatgagcgta cgaatggtcc gcatttcggg gttcctccct
     1861 gatctgcgag accgctgtcc ccggtggctc ggccgcgcct cgagggcgga cgccggcaac
     1921 aaaaagccag cgtccgagca cctcgaaaac gctggcctga tgttcggaga ccgaaccgca
     1981 ttcctacgca cggatgaccg tgatcaggtt cgaggggttg gtggtcgccc acctctcagc
     2041 ccgaaggcac ccccagcgga tctctgcgct gtgactgctg ccgagggtac cgcgctcgcg
     2101 ctcgccgtca agaccgcgcc ggcagccgct ccgcgatcgc ccgcgccgac gccgccggtg
     2161 gcagcgtcgc atcgagcacg aggtgcgggc cttcgctcgt ccccaccgcc tcgaactgtc
     2221 cgcgctggcg ctcgtacacc tcccacgtcg cgtccgaggt gcgcagcggg tcgtcctcgc
     2281 ggccctcgat ccgcgcccgc gccgcctcgt cgtcgagccg caactccacg atcagcgccg
     2341 gcacgccatg ggctcgcgcc atctctatcg ccgctgcccg ctccgaggcc cggccatgca
     2401 tcgcgtcgat caccaccggc cgccccgcct cgaggtgtac gtgcgcacgc gttcgcagct
     2461 cctcgtacgt gcgctccgtc atctccgcgt tgtacagccc gctccgaaac tccgctgcgg
     2521 cccgttcccg cgggttcagc cccgccagct gcttacgcgt cgcgtccgag gacaggtacg
     2581 cggccccgat ccggccggcg agcgtcgccg ccaccgtcga cttcccggtc gccggcagcc
     2641 cgctcatgac cacgacgcac ggcgccgccc ggcgcccgcc gaacgacgcc gcgaggcgga
     2701 agtacgccgc cgccgaggcc cacagccgct cccgctcctc agtcgagatt tccgtggcat
     2761 gcgccccgat cgactcgacc ttcccgcgca cgaacgcgcg gaacgcccgg tgcaacggtt
     2821 gcagcacacc catcgtctcg tcgcacgccg ccgcgatgta ccgccccgcg acctcgtccc
     2881 cgagctccgg gaacccccgc gcctcgaggt ccatcgcgag gaacgcgatg tcgtacccca
     2941 cgtcgaggta ccggaagtgg aaccagtccg tgaattcgat gcagtccacg atcgcgacct
     3001 gctcgccgcc ctcgaggagg tagacgtgct tcgcgtgcag gtcgccgtgc ccctcgacga
     3061 cgcgcccgtc ggcgagccgc tggtcgaaca gcgcctgctg cgcctcgatc gtctcgtcga
     3121 agaacgcctt cgtcgcctcg gcatcctcgg cacgccaggt gtcaccgatg aacccggctg
     3181 cctccccgta ctcgcgcgac caccatttcc gttcacccgg cccgcccgcg tactcggggt
     3241 cgttcggcac cactgcggcg gcctcgtgaa acgcgatcag cgtttgcacg agccgctccg
     3301 cgagccgagg cggcggtccc tcccctgcca ggatgcggtc gagcgtgcgg tcgtccggca
     3361 ggcggcgcat cttcaccgcc cactccacga cctcgccctc cgactccacc gcgaaccgcc
     3421 cgtccgcgcg ccgcaccaca ggcaccaccc cgaggtacac atcgggagcc agccgcgagt
     3481 tcagacgtac ctcgtcgtgg cagtactgct cgcgcgcctc gggcgtcagc tgactgatga
     3541 acccgaagtc gaccggcttc ttggtcttgt agaccacgtc gcccgccagg aagacatacg
     3601 agatgtgcgt ctgcgccagc tccaccgagg ccggtggctc ggggtacgcg gacggctcga
     3661 ggaggccgcg gacatacggg ggcagcgtgt cgtcgctcac ctcgcgacgg taggcacgcc
     3721 gcgaggggga gtcaatcgcc ggagctggat cgccctatgc gtatagctcg aggtgttcct
     3781 tctgcagccg gtccccgaac gccgcaatca acgtgtcgaa ctccgccacg tcacgttcgc
     3841 acacgaaggt gatcgccttg cccgtccgcc ccgcccggcc cgtccggccg atgcggtgga
     3901 cgtactcctc ggggttctgt ggcacgtcgt agttgagcac gtgcgtgatc tcggggatat
     3961 cgagcccgcg cgcggccacg ttcgtcgcca cgaggatgtc gagctgcccg ctgcggaact
     4021 gctgcacgac gtggtcgcgc ttccgctggt ccatgtcccc gtgcagcgcg ccgacccgaa
     4081 tccccgcgtc cgagagctgg tcgcgcaggt agtccacccc gcgccgggtc cgcgagaaga
     4141 cgagcgtccg cccgcgcagc tcgcgttcaa tcagctcgcg cagcccacgg atcttgtcgc
     4201 gctgagcgac ctcgaagtac acctgctcga ccgtttcgac ggtcgtctgc tccggcgcga
     4261 tcgtgaccgt ctcgggctcc tccatgtacc gccgcacgag gcgcaggatg gagtgtggca
     4321 tcgtcgccga gaacagcgcc gtctgccgtg cccgcggcgt gcgcgtgagg atgcgcgtga
     4381 tgtccggcag gaacccgatg tccagcatct ggtccgcttc atcgaggacc gcgtaacgca
     4441 ctgcgttcag tgacagcgtc tgccggtgca ggtggtcgat cactcgcccg ggcgtcccca
     4501 ccacgatctg cggctgccgc tccagcgctc ggaagtcccg gtcgagtgac cggcccccga
     4561 gcagtcccac ggcctcgagg ccgtagtacc ggccgaggtc ttgcagcacc tcgagtacct
     4621 gctgtgccag ctcgcgtgtc ggcaccagca ccagcccctg caccgcgcga tgccccgcgt
     4681 cgagccgctc gaccatcggc aacccgaaag cgagcgtctt tccggtcccc gtctgcgcga
     4741 tgcccacgac gtcccgcccg ctcaggagca acgggatcgc ctgctcctgg atcgggctcg
     4801 gcgtcgtaaa gccaaagttc gtgaccgccg cgagcgatcg ctcgttcaac ccgagcgcga
     4861 cgaacgcggg cgggaacgag gcagggtcga cctcgatcgg aggaggagca tgccgctccc
     4921 gctcctccgc ctgcacggcg acggcatcct cgtacgtcgg ctcttcgtcg tcctcggagg
     4981 cgtcctccgc ctcgtccgag tccgcttccg aagcggcttc gccctcgacg ccttccgcgt
     5041 gccggtgtcg ccggccccca cgccggccac gccggcggcg cttcgaggtg gcttcctcgt
     5101 cgacgccctc gccggtctca tcggcggtgg tgggcggtgg ctcgggcggc ccttcgtgct
     5161 cttcggcttc cacacccggc tcggactccg ccgagcgcgt tacgtactgc ttgatccccg
     5221 cgtacagcac gcggtcggtg cgcggcccca ccggccggcg ccgcaccggc acgatcgagg
     5281 cgtccccgcc ctcggccgcc ggacggaacg cgatgtcctc cggcaacggc gcatcgagcg
     5341 aggacaacgc gggtcgccgc caccgcccgt ttcgaggagc ttccgaacgg cccccggatc
     5401 gagcgggcgc gggcgctgtg gcgccgttgc cattcccgt
//
LOCUS       DAORPG010000046         5433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_892_length_5433_cov_3.554295, whole genome shotgun sequence.
ACCESSION   DAORPG010000046 DAORPG010000000
VERSION     DAORPG010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_892_length_5433_cov_3.554295"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2509)
                     /locus_tag="PLX85_01805"
     CDS             complement(<1..2509)
                     /locus_tag="PLX85_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015149701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HRC61943.1"
                     /translation="MGAPGLPQAQGLYDPIHEHDSCGVGFIVHLKGKRSHQIVRDGLT
                     ALENLNHRGACGCEVNTGDGAGILIQVPDEFFRHECAHLGIRLPEAGHYGVGLLFASR
                     NEHARHEAMNLFASIVEEEGQHLLGWRDVPTDAKAADVGPSALAVEPAMLHVFIGRGA
                     DLPDDDAFERRLYVIRKRFEKAIERSDVHDAPYFYFPSLSSRTLVYKGMLTATQLSSY
                     FPDLADPRMVSALAMFHSRFSTNTFPSWKLAHPYRMISHNGEINTLRGNRNWMAAREA
                     LFDSPLFDEVGKIAPVIDEAGSDTASLDNALELLFQSGRPLAHSMMMLIPEAWSGHES
                     MPAEKQAFYEYHSTVMEPWDGPASVAFTDGRSIGAVLDRNGLRPSRFYVTKDDLVIMA
                     SEVGVLPIEPERVLTKGRLQPGKMFLVSLDEGRIIDDTELKMGLASQRPYADWLNEHL
                     HPLSTLPAGEAAPGLGGDDLLKHQMAFGYTIEDLKYILGPMVTNSEEAIGSMGTDTPL
                     AVLSNRAQPLYNYFKQLFAQVTNPPLDAIREELVTSVDTLIGPEGNLLDTGLSDIHLV
                     KLESPFIDNEDLARIKALADDPRFRTRELSTLFSVEDGPRGMEEAIEALFERADEAIE
                     AGSKILVLSDRGVDTEQAAIPALLAVSGLHNHLVRQKKRTQVGLVLETGEPREVHHFA
                     LLVGYGAGAVNPYLALDSLSRLQEDGYVDPALDVHEAHKHYLKALKKGVVKVMSKMGI
                     STVQSYRGAQIFEAIGLSQEFVDRYFTKTVSRIEGVGLEQIAEETLTHHEHAFPRRDG
                     GLHELNWGGQYQWRRDGEFHLFNPDTVFRLQHATRTGR"
     gene            2781..3686
                     /locus_tag="PLX85_01810"
     CDS             2781..3686
                     /locus_tag="PLX85_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRC61944.1"
                     /translation="MKIVWATDGSAGSEGALPYLNGLFNRRDNEVIVTAVAPAPLLSA
                     ARPDPSMLLWHLVPGYRDRVASEVTDLVVRQVGLLAKSRATVSSAVRLGSAPPELILL
                     ARDERADLIITGARGHTPASEILLGSVSQQVATNADCSVLVVRGRKRPARVLLAYDGS
                     VDAEAGLTLLSSLAPTKGMEAVVTTVAEPVMPPLDAPPGWAPDTVAEADRWRKTSARR
                     HAQAAVRRLRAAGWTASTHIGEGHPGSAILQAAREMAADLVVIGARGIHSPDEETRGM
                     GGIPRQVLERAASSVLISRAPRTEG"
     gene            complement(3690..4943)
                     /locus_tag="PLX85_01815"
     CDS             complement(3690..4943)
                     /locus_tag="PLX85_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRC61945.1"
                     /translation="MTTESPRPQLNAGPLHGLRVLDLSWVLAGPYCTMTLCDLGAEVV
                     KCERPPFGDVARTTGPLIDGESGYFFSVNRGKKSIAIDLKQDEGKALFKRLVREFDIL
                     VENFRPGTLDDLGVGYEVLSQEHPGLIYVAISGFGQTGPMREQPALDVVVQGAGGIMS
                     VTGEPGGPPIKPGVSLGDITGGLYGAIGALAAVHERERSGKGQLVDVSMLDCQVAILE
                     NAFLRYFLTGEPPEPLGTRHSWAVPFQAFPTADGYFVLALAWGVPNQWALLCSELDRV
                     DLIDDPRFATSTARAAHHAELEPILNEEFRKRPGSHWIERLTQYGIPCGPLNSIPEVT
                     RMPQLEAREMFVPVQHRRLGTLPLVNSPIKLSRTPAGIRGSSPDMGEHSRAVLHDRLG
                     LSDEEIDALAAREVVWEERPPVDLG"
     gene            complement(5041..5331)
                     /locus_tag="PLX85_01820"
     CDS             complement(5041..5331)
                     /locus_tag="PLX85_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61946.1"
                     /translation="MAVSTELQHGGQLMRNVHLNPLVRSHGSLRVVGSRGPAIRQFAS
                     RQTHDRPLLVAGSLLVLGLLPVAAYASRRAFGRPQPDAAELLPRNQEWYPWE"
BASE COUNT          884 a         1702 c         1936 g          911 t
ORIGIN      
        1 cgcggcccgt gcgcgtggcg tgctggaggc ggaacacggt gtcggggttg aagaggtgga
       61 actcaccgtc acggcgccac tggtactggc cgccccagtt gagctcgtgg aggccgccgt
      121 cgcgcctcgg gaaggcatgc tcgtggtggg tcagcgtctc ctcggcgatc tgctcgaggc
      181 cgacgccttc gatgcgtgac accgtcttcg taaagtagcg gtcgacgaac tcctgggaga
      241 ggccgatcgc ctcgaagatc tgcgccccgc ggtaagactg gacggtggag atgcccatct
      301 tggacatcac cttcacgacg cccttcttga gcgccttcag gtagtgcttg tgcgcctcgt
      361 ggacgtcgag ggcggggtcg acgtagccgt cctcctgcag ccgggacagg ctgtcgaggg
      421 cgaggtacgg gttgaccgcg ccggcgccgt agccaacgag gagcgcaaag tggtgcacct
      481 cgcggggctc gccggtttcg aggacgaggc cgacctgcgt gcgcttcttc tgacgcacga
      541 ggtggttatg gaggccggac accgcgagca gcgcgggaat cgccgcctgc tccgtgtcga
      601 cgccgcggtc ggatagcaca aggatcttcg agccggcctc gatggcctcg tcggcgcgtt
      661 cgaagagtgc ctcgatagcc tcttccatcc cgcgcgggcc gtcttcgacc gagaagagcg
      721 tggacagctc gcgcgtgcgg aagcgcgggt catcggcgag cgctttgatc ctcgcgaggt
      781 cctcgttgtc gatgaagggc gactcgagct tcacgaggtg gatgtctgag aggcccgtgt
      841 cgaggaggtt gccctcgggg ccgatcaggg tatcgaccga ggtgaccagc tcctcgcgga
      901 tggcgtcgag cggcgggtta gtgacctggg cgaagagctg cttgaagtag ttgtagagcg
      961 gctgcgcgcg attcgagagc acggcgaggg gcgtgtcggt gcccatcgag ccgatggctt
     1021 cctcggagtt cgtcaccatg gggccgagga tgtacttgag gtcctcgatg gtgtagccga
     1081 aggccatctg atgtttgagg aggtcgtcgc cgccaagacc gggtgccgcc tcgcccgcag
     1141 ggagcgtgga cagcgggtgc aggtgctcgt tgagccagtc agcgtacggc cgctgcgatg
     1201 cgaggcccat tttgagctcg gtgtcatcga taatgcggcc ctcgtcaagg ctgacgagga
     1261 acatcttgcc cggctgcagg cggcccttgg tgagcacacg ctcaggttcg atgggcagga
     1321 cgccgacctc ggatgccatg atcacgaggt cgtccttcgt cacgtagaag cgagacggac
     1381 gaaggccgtt gcggtcgagg acggcgccga tggagcggcc gtccgtgaag gcgacggagg
     1441 cagggccgtc ccatggctcc atcaccgtac tgtggtactc gtagaacgcc tgcttctcgg
     1501 cgggcatgga ctcgtggcca ctccacgcct cggggatgag catcatcatc gagtgcgcga
     1561 gcggacgccc gctctggaag agcagttcga gggcgttgtc gagggaggcg gtgtcgctgc
     1621 ccgcctcgtc gatgaccggc gcgatcttgc caacctcatc gaagagcgga gagtcgaaca
     1681 aggcctcacg ggcggccatc cagttgcggt tgccgcgaag ggtattgatc tcaccgttgt
     1741 gggagatcat tcggtacggg tgagcgagct tccagctcgg gaaggtgttc gtgctgaaac
     1801 gggagtggaa catcgcgagg gcgctgacca tgcgcgggtc ggcaaggtcc ggaaagtagc
     1861 tcgagagctg tgtcgcggtg agcatgcctt tgtagacgag ggtgcgcgaa gaaaggctcg
     1921 ggaagtagaa gtacggcgcg tcgtggacgt cagaacgctc gatggctttc tcgaagcgct
     1981 tgcggatgac gtagaggcgg cgctcgaagg catcgtcatc aggaaggtcc gcgccgcggc
     2041 caatgaagac gtgcagcatc gcgggctcga cggcgagcgc gctcgggccg acgtcggcag
     2101 ccttcgcgtc ggtcgggacg tcgcgccagc cgagcaggtg ctgcccctct tcttcgacga
     2161 tcgaggcgaa gaggttcatg gcctcgtggc gagcgtgctc gttcctcgag gcgaacaaga
     2221 ggccgacgcc gtagtggccc gcctccggga ggcgaatgcc gaggtgcgca cactcgtggc
     2281 ggaagaactc gtcggggacc tggatgagga tgcccgcgcc gtcgccggtg ttgacctcgc
     2341 agccgcaggc gccacgatgg ttgaggtttt cgagcgcggt gaggccgtcg cggacgatct
     2401 ggtgcgaccg cttacccttg aggtggacga tgaacccaac cccgcagctg tcgtgctcgt
     2461 ggatcgggtc gtacaggccc tgagcctgtg gcaatccggg cgcgcccatc tcgctccctc
     2521 cccctgccac cgatgacgac tccgctgcgg tatcggcgcc gcttcgccga tgtaccgctg
     2581 tcctcggtgg ccgcgttgac ggttcagatc gtcgatgtgg agttcatcgc cgagaagtga
     2641 acgacgatca tacgcgatcc tgattcccct ggggatcagg atgagcggtc atattgcgcg
     2701 tttcgtcaca aactcgcaag agggggaacg gcggcggccc ctctctcgcc cgttcgagga
     2761 ttctccctat agtggacgcg atgaagatcg tgtgggcgac ggatggatcg gccggctcgg
     2821 agggcgcgtt gccctatctg aacggcctct tcaatcgcag ggacaacgag gtcatcgtga
     2881 ctgcggtggc gccggcgccg ctgctgtccg ccgcccgacc cgacccctcg atgctgctct
     2941 ggcacctggt gccgggctac cgcgaccgtg tcgccagcga ggtaacggac ctcgtggtgc
     3001 gccaggtcgg gctgctcgcc aagtcgcgcg ccaccgtcag cagcgcggta cgcctcggat
     3061 cggctcctcc ggagctcatc ctcctggctc gcgacgagcg cgccgacctc atcattactg
     3121 gcgcgcgggg ccacacgccg gcgtccgaaa tccttctggg gtctgtctcg cagcaggtgg
     3181 cgacaaacgc ggactgctcg gtgttggtcg tacgtggccg caaacggccc gcgcgtgtgt
     3241 tgcttgcata cgacgggtct gtcgacgccg aggcaggcct cacactgctg tcgtccctcg
     3301 cgccaacgaa gggcatggaa gcggtcgtta cgaccgtggc cgagccggtc atgccacccc
     3361 tcgacgcgcc gccgggatgg gcaccagata ccgtcgcgga ggccgaccgc tggcgcaaga
     3421 cgagcgcccg gcgccacgcg caggcggcgg tacgccggct acgggcggct ggttggacgg
     3481 ctagcacgca tatcggcgag gggcatcccg gcagtgccat cctccaggca gctcgcgaaa
     3541 tggccgccga cctggtggtc atcggggcgc gaggcatcca ctcgcctgat gaggagacgc
     3601 gcggcatggg agggatcccc cgccaggtcc tcgagcgggc cgcgagctcg gtcctcattt
     3661 cccgcgcgcc gcggaccgag gggtagcgct cagccgaggt cgaccggcgg ccgctcctcc
     3721 cagaccacct cgcgtgccgc gagcgcatca atttcctcgt cactgagtcc cagccggtcg
     3781 tgcaggaccg cccggctgtg ctcgcccatg tcgggcgagc tgccccgtat gcccgcggga
     3841 gtgcgcgaga gcttgatcgg tgagttcacc agcggcaggg tcccgagtcg ccgatgctgt
     3901 acaggaacga acatctcgcg ggcctccaac tggggcatgc gggtgacctc gggaatcgag
     3961 ttcagcggac cgcaggggat gccgtattgc gtcagccgct caatccagtg ggagcccggc
     4021 cgcttgcgga actcctcgtt gaggatgggc tcaagctcgg cgtggtgcgc ggcgcgcgct
     4081 gtgctcgtgg cgaagcgcgg gtcgtcaatg aggtccacac ggtcgagctc ggagcagagc
     4141 agcgcccatt ggttgggcac accccaggca agcgcgagga caaagtagcc gtctgccgtc
     4201 ggaaacgcct ggaacggcac ggcccatgag tgcctggtgc cgaggggctc aggcggctcg
     4261 ccagtgagga agtaacggag aaaggcgttc tcgaggatcg caacctggca gtcgagcatc
     4321 gagacgtcga cgagttggcc cttccccgaa cgttcgcgct cgtggacggc ggcgagcgcg
     4381 ccgatggcgc cgtagagacc accggtaatg tcccccagcg agaccccggg cttgatcgga
     4441 ggaccgccag gctcgccggt cacggacatg atgccgcccg cgccctgcac gacgacgtcg
     4501 agcgccggct gctcacgcat cggaccggtc tggccgaaac cggagatcgc cacgtagatc
     4561 agtccggggt gctcctgcga gagcacctcg tagcccacgc cgaggtcatc gagggtgccg
     4621 ggccgaaagt tctcgaccag gatgtcgaac tcgcgaacga ggcgcttgaa gagcgctttg
     4681 ccctcatcct gcttgaggtc gatggcgatg ctcttcttgc cccggtttac gctgaagaag
     4741 tagccggact ctccgtcgat cagggggccc gtcgtcctcg cgacatcgcc gaagggcggc
     4801 cgctcgcact tcaccacctc ggcgccgagg tcacacagtg tcatggtgca gtacggaccg
     4861 gcgaggaccc atgacaggtc gaggacgcgg aggccgtgca acgggccggc gttcagctgc
     4921 gggcgcggag attcggtcgt catggcgtaa tccgtctctg tgttctcgtg cggtgatggg
     4981 cagcataaag gccgccggct tgcaccggcg gcctcgtgca ttgcggacag gcgtcccggg
     5041 ctactcccag gggtaccact cctggttcct cggcagcagt tcggcggcgt ctggctgcgg
     5101 gcggccgaac gcacgcctcg aggcgtaggc ggccaccgga agcaggccga gcaccaacaa
     5161 ggagccggcc accagcagcg gtcggtcgtg cgtctgacgc gaggcgaact ggcggatcgc
     5221 cgggccgcgc gagccgacca cgcgcagcga cccgtggcta cgtacgaggg gattcaggtg
     5281 cacgtttcgc atgagctgcc ctccatgctg cagttccgtg gatacagcca tgatagcgca
     5341 ccggcgccgc ggatcccttg aagcgcatgg cgatccttgg cccgacacgc ccgggaagcg
     5401 tcgccgacgc attgacaagc ttcttcgcga agc
//
LOCUS       DAORPG010000047         5395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_908_length_5395_cov_3.352996, whole genome shotgun sequence.
ACCESSION   DAORPG010000047 DAORPG010000000
VERSION     DAORPG010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_908_length_5395_cov_3.352996"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PLX85_01825"
     CDS             <1..478
                     /locus_tag="PLX85_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61947.1"
                     /translation="VALRAITTSHMPPIVSTPPVEAEREAPPPPFVPPDAADAPPALR
                     EIRGKWGGIREAVRKRSHKAGALLNTACDIKSFDGDSVELGFRFPTHVQKAQEDPKVM
                     QAIREAVAEAVGHPVQVVPVVWDAMQQAAAPPPQAQSRGGHLLEEALRYGAVPVDN"
     gene            491..1111
                     /gene="recR"
                     /locus_tag="PLX85_01830"
     CDS             491..1111
                     /gene="recR"
                     /locus_tag="PLX85_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRC61948.1"
                     /translation="MQDPWPSSTSPIQRLVEAFHRLPGIGPKSAQRLAYYILRAPEEE
                     ARALAEALLEVKERVVLCSRCQDITDQDPCRICLDTSRDRTSICVVEEPLDLRAIERT
                     RGYHGLFHVLHGVISPADGVGPEDLKIAELLARLRDVDHDATEVIVATNPNLEGEATA
                     MYLGRLLTPLGIRVTRLARGLPAGADLEYADDVTLGRALEFRQVVE"
     gene            1114..1326
                     /locus_tag="PLX85_01835"
     CDS             1114..1326
                     /locus_tag="PLX85_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61949.1"
                     /translation="MKLWSGLGKWWWVAFVVTWVVVTVVLNNTGTSDTASVRVAGVVA
                     AIVAALVWLAARMLGGRPQGRGPGPS"
     gene            1329..2150
                     /gene="recO"
                     /locus_tag="PLX85_01840"
     CDS             1329..2150
                     /gene="recO"
                     /locus_tag="PLX85_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRC61950.1"
                     /translation="MSARPPRVTRTEALVLRHRRLGEADRIVTLLTPARGKVDAVAKG
                     TLRPKSRLAGHLEPLIQVEVLLAHGRNLDIVTQAQMIEGFPALHADLERLSTAMYLAE
                     LADRMTVEHAEAHGIYELLLASLVRLSRGDGVHLVTRTFEMELLATTGFRPEWRDCIG
                     CGDAVAAERATWSPLGGGVVCPRCRERHNDALAIDATVLKVLRAIQAGPYEEAGRIRL
                     NSELAGGLERVMHTLVRAVSERALTSAQFVADARRLAAAGVRGDAYTRNDLTEGD"
     gene            2153..2536
                     /locus_tag="PLX85_01845"
     CDS             2153..2536
                     /locus_tag="PLX85_01845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRC61951.1"
                     /translation="MPLYEYYCTNCNGIFELLRPISQATDPQPCVVCDSESKRIMPTE
                     FAAFVMRDGMPRRIPDQGLSWGLEGQLKNPLTSSEAKKEDIVPEERKRRASTVLSEDE
                     KVASKPEIRVRPRKKKSTAKAKSAK"
     gene            complement(2543..3520)
                     /locus_tag="PLX85_01850"
     CDS             complement(2543..3520)
                     /locus_tag="PLX85_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRC61952.1"
                     /translation="MTTRTDVTAIYGPVAGDLPRVDQMLTSLQHVEFPWLRSMLEATL
                     TGGGKRLRPAIALLAGRLGDYDLDKLVPLAASVELLHTATLIHDDVIDAAQERRGKPT
                     AAALFNNASSVMLGDYMFAHAAEFVARTGNIRVIRNFADTLRVMANGELTQDMSAYEY
                     SADVQRYLDRIYGKTASLFATAAEGGAIVTGAPEEFVAPMRSFGEHLGMAFQIVDDIL
                     DFSATSEELGKPAGSDLLSGTLTLPTLLYMERYPDDNPVRRAFDGTRRRANLARAIDE
                     IRDSDILEESMKTAERFALHARDALERIPSGDPRTTLDGLVDYVLSRRS"
     gene            complement(3527..4318)
                     /locus_tag="PLX85_01855"
     CDS             complement(3527..4318)
                     /locus_tag="PLX85_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRC61953.1"
                     /translation="MVDAHAHTYSRQFDDDRDATFQRAWDAGLVAVVEVGGDVDSSRS
                     ALALSDADPRVHAVAGIHPHEAQHLDTEREELRALVGTGRFVGVGEIGFDFHYNHSPR
                     EAQFAAFLWQLDLAREAELPVVIHCREADDDGFAVLSEWARRVGRYLGPEREIGMLHC
                     FAGNAELAQRYIELGFLISIPGPVTYKNNDRGQLVARSVPLTAMLVETDAPYLTPNPH
                     RGTRNEPAYVLETIRMVAQLRGCTPEEVATATAQNAGRLFGFRIE"
     gene            complement(4327..>5395)
                     /locus_tag="PLX85_01860"
     CDS             complement(4327..>5395)
                     /locus_tag="PLX85_01860"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRC61954.1"
                     /translation="REWVERQEHWRPAVRNFTLGLLREGLHDRGITRDLDWGIPVPVE
                     GFEGKRIYVWFEAVIGYLSASIEWASRNGTPEAWRDFWEDPSARTYYFQGKDNVPFHT
                     TIWPAMLMGRGGLNLPYDVPANQYVTMSGAKASSSRNWAVWMPDYVARHDPDPLRYML
                     SAIMPETSDSDFTWAEYVRRNNDELVATWGNLVNRVLTFTRRNFDARVPEPRAMLAPE
                     SQAILDAVDAAFEEVGQHIEAVRLRAALQAAMSVAQQTNRYLDERAPWSAIKQDREAA
                     AETLYTAINVISGLASLFQPFMPFTSPVAWSLGGNQGDIEGAGWRRSPVPAGTSLPEP
                     SPLFKKLDDALVDEEEARLGR"
BASE COUNT          868 a         1748 c         1896 g          883 t
ORIGIN      
        1 cgtcgcgttg cgcgcgatca ccacctcgca catgccgccg atcgtctcga cgccaccggt
       61 tgaggcggag cgcgaggcgc ccccgccgcc attcgtccca cctgacgccg cggacgcgcc
      121 tccggccctg cgggagattc gcggcaaatg gggcggcatc cgcgaggcag tgcggaagcg
      181 ttcgcacaag gcgggcgctc tgctgaacac cgcgtgtgac atcaagagct tcgacgggga
      241 cagcgtagag ctcgggttcc gcttcccgac gcacgtacag aaggcgcagg aagacccgaa
      301 ggtcatgcag gcgatccggg aggcggtggc cgaggcggtt gggcatccgg tgcaggtggt
      361 acccgttgtc tgggacgcca tgcagcaggc tgccgcgccg ccgccgcagg ctcagtcacg
      421 aggcggccat ctcctcgagg aggcgctgcg gtacggcgcc gtcccggtcg acaactagcg
      481 gacgcggccg ttgcaggacc cctggccatc ctcgacctct ccgattcaac gcctcgtcga
      541 ggcgtttcac cgtctgcccg ggatcggtcc gaagtcggcg caacgtctgg cgtattacat
      601 cctgcgtgcg cccgaggaag aagcgcgtgc gctagcggag gcgctcctcg aggtgaaaga
      661 gcgggtcgtc ctgtgctcgc gctgccagga cattaccgac caggatccgt gccggatttg
      721 ccttgacacg tctcgcgacc gcacctcgat ctgcgtggtg gaggaaccgc tcgacctgcg
      781 ggcgatcgag cgcacgcggg gctaccacgg gctgtttcac gtgctccacg gcgtgatctc
      841 gcccgctgac ggcgtcggcc ccgaggacct taagatcgcc gagttgctcg cgcggctgcg
      901 agacgtcgac catgacgcga ccgaggtgat cgtggcgacg aacccgaacc tcgaaggcga
      961 agcgacggca atgtacctgg gacggctgct cacgccgctc gggatccgcg tcacccggct
     1021 tgcgcgaggc ctgcccgccg gggccgacct cgaatacgct gacgacgtga cgcttgggcg
     1081 agcgctcgag ttccggcagg tggtggagta gcggtgaagc tgtggagcgg cctcgggaag
     1141 tggtggtggg tcgccttcgt ggtcacgtgg gtcgtcgtca ccgtcgtgct aaacaacacc
     1201 ggcacgagtg acacggcgtc cgtccgggtc gcgggcgtcg tggcggcaat cgtggcggca
     1261 ctcgtctggc tggccgctcg catgctcggc ggccgtccgc agggccgcgg ccctggcccg
     1321 agctagcgat gtccgcgcgt ccgcctcggg tcacccgaac ggaggcgctg gtattgcgcc
     1381 accgacgcct gggagaagct gaccgtatcg tgacgctgct cacaccggct cggggcaagg
     1441 tcgacgcggt cgcgaagggg acgctgcggc caaagagccg ccttgcgggg cacctggagc
     1501 cgcttattca ggtcgaagtg ctgctggcgc acgggcggaa tctcgacatc gtgacgcagg
     1561 cgcaaatgat cgaggggttt cccgcgctgc atgcggatct ggagcggttg agcaccgcga
     1621 tgtaccttgc cgagctggcc gaccggatga cggtcgaaca tgccgaagcg cacggtatct
     1681 acgaactgct gctggcctcg ctcgtacgac tcagccgggg cgacggagtg cacctcgtga
     1741 cccgcacctt cgagatggag ctgctcgcga cgaccggctt ccgcccggag tggcgggact
     1801 gcatcggttg cggcgatgca gttgccgccg aacgtgcgac gtggtcacca ctcggcggcg
     1861 gcgtcgtttg ccctcgctgc cgcgagcgcc ataacgatgc tctggccatc gatgcgaccg
     1921 tactcaaggt cctgcgcgct atccaggcgg gaccgtacga ggaagccggt cggattcgcc
     1981 tcaactcgga gttggcgggc ggcctcgagc gcgtgatgca cacgctcgtg cgcgcggtga
     2041 gcgagcgggc gctgacgagt gcccagttcg tggccgacgc acgccggctg gctgctgccg
     2101 gcgtccgagg ggacgcctat actcgaaatg acctgacaga gggggactga ccgtgccgct
     2161 atacgagtac tactgcacaa actgcaatgg catcttcgag ctgttgcgcc cgatcagcca
     2221 ggcgacggac ccgcagccat gtgtggtctg cgactccgag tcgaagcgca tcatgcccac
     2281 cgagttcgcc gcgtttgtca tgcgcgacgg aatgccccgc cgtatcccgg accaggggct
     2341 gtcgtggggc ctcgaggggc agctgaagaa cccactcacc tcgagcgagg caaagaagga
     2401 agacatcgtc ccggaggagc gcaagcggcg agcgagcacg gtgctcagcg aggacgaaaa
     2461 ggtggcgtca aagcccgaaa ttcgcgtccg gccgcgcaag aagaagtcga cggcgaaagc
     2521 aaagtcggct aagtagaacc gactaggagc ggcggctcag cacgtagtcg acgaggccgt
     2581 cgagggtcgt cctcggatca ccgctcggga tccgctccag tgcgtcgcgg gcgtgcaggg
     2641 cgaagcgctc cgccgttttc atcgactctt caaggatgtc ggaatcgcgg atctcgtcga
     2701 tggctcgcgc cagattcgcg cgccgtcggg tgccgtcgaa tgcgcggcgg acggggttgt
     2761 cgtccgggta ccgctccatg tagagcagcg tcgggagtgt cagggtgcct gacagcaggt
     2821 cggaacccgc cggcttgccg agctcctcgg acgtcgcact gaaatcgagg atgtcgtcca
     2881 caatctggaa cgccatcccg aggtgttcgc caaagctgcg cataggcgcc acgaactcct
     2941 cgggcgcccc ggtcacgatg gcaccgcctt cggcggcggt cgcgaagagc gaggcggtct
     3001 tgccatagat gcggtcgagg taccgctgga cgtcggcgga gtactcgtag gcgctcatgt
     3061 cctgcgtgag ctcgccgttg gccatgacgc gcagggtgtc ggcgaagttg cggatgaccc
     3121 ggatgttccc ggtccgggcc acgaactcgg ccgcgtgggc gaacatgtag tcgccgagca
     3181 tgacgctcga ggcgttgttg aacagcgccg cggcggtcgg cttccctcgg cgttcctggg
     3241 cggcgtcgat cacgtcgtcg tgaatcaggg tggcggtgtg cagcagctcg acgctggcgg
     3301 cgagcgggac gagcttgtcg aggtcgtagt ccccgaggcg ccccgccagc agcgcgatcg
     3361 cggggcggag ccgcttcccg ccgcccgtca gcgtcgcttc gagcatgctg cgcaaccacg
     3421 ggaactccac gtgttggagc gaggtaagca tctggtcgac acgtgggaga tcccccgcga
     3481 ccggaccata gattgctgtc acgtcggttc tcgtggtcat gcgctgctac tcgatgcgga
     3541 agccgaagag gcggcccgcg ttctgcgcgg ttgcggtcgc gacctcctcc ggcgtgcatc
     3601 cgcggagctg tgcgaccata cgaatcgtct cgaggacgta cgcaggctcg ttgcgcgtgc
     3661 cgcgatgcgg gttcggggtg aggtagggcg cgtccgtctc taccagcata gctgttaacg
     3721 gcaccgaacg ggcgacgagc tggccccggt cgttgttctt gtacgtgact ggcccgggaa
     3781 tggagatcag gaagcccagc tcgatgtagc gctgcgccag ctcggcgttg cccgcgaagc
     3841 agtgcagcat tccgatctcg cgctcgggcc cgaggtagcg tccgacacgc cgcgcccact
     3901 cgctgaggac cgcgaagccg tcgtcgtccg cctcgcggca atggatcacg acgggcagct
     3961 ccgcctcgcg cgccaggtcg agctgccaca ggaacgcggc gaactgcgcc tctcgcggcg
     4021 aatggttgta gtggaaatcg aagccgatct cccccacgcc cacgaatcgg ccggtgccaa
     4081 cgagcgcgcg cagctcctcg cgctcggtat cgaggtgctg cgcctcgtgg ggatggatcc
     4141 cggcgacggc atggacccga ggatcagcgt cgctgagggc gagcgcgctg cggctgctgt
     4201 cgacgtcgcc gccgacctcg acgaccgcga cgaggcccgc gtcccaggcg cgctgaaacg
     4261 tcgcgtcccg gtcatcgtcg aactgacggg agtaggtgtg agcgtgcgcg tcgaccaccg
     4321 gagcgttcac cggccgagcc gcgcttcttc ctcgtcgacg agggcgtcat ccagtttctt
     4381 gaacagaggg gatggctccg ggagcgatgt gcccgccggg actggcgacc gccgccaccc
     4441 cgcgccctcg atgtcgccct gattgccccc gaggctccac gcgacggggg aggtgaatgg
     4501 catgaagggc tggaagaggc tcgcgagtcc gctgatgacg ttgatggcgg tgtacagcgt
     4561 ttctgctgcc gcctcgcgat cctgcttgat ggcgctccag ggcgcccgct cgtccaggta
     4621 gcggttcgtc tgctgcgcga ccgacatcgc ggcttgcagt gcggcacgca gccgcacggc
     4681 ctcgatgtgt tggcccacct cctcgaacgc ggcgtcgacc gcatcgagga tcgcctgcga
     4741 ctccggggcc agcatcgctc gcggttcggg cacgcgagcg tcaaagttgc gccttgtgaa
     4801 cgtgagtaca cggttcacga ggttgcccca ggtggcgacg agctcgtcgt tgttgcggcg
     4861 aacgtattcg gcccaggtga agtcggagtc ggacgtctcc ggcatgatcg ccgacagcat
     4921 gtaccgcagc ggatcgggat cgtgacgggc gacatagtcc ggcatccaga cagcccagtt
     4981 gcggctcgat gatgccttgg cgcccgacat cgtgacgtac tggttggccg gcacgtcgta
     5041 cgggagattc aggccgccgc gtcccatcag cattgccggc cagatcgtgg tgtggaacgg
     5101 cacgttgtcc ttcccctgga agtaatacgt gcgcgccgac gggtcctccc agaagtcgcg
     5161 ccacgcctcg ggggtgccat tcctcgaagc ccactcgatc gaggcggaga gatagccgat
     5221 cacggcctca aaccaaacgt agatacgctt gccctcgaag ccctcgacgg gcacggggat
     5281 gccccagtcg aggtcgcgcg tgatcccgcg gtcgtgcagg ccttctcgga gcagcccaag
     5341 cgtgaagttg cggacggccg gtcgccagtg ctcttgccgc tcgacccatt cgcgc
//
LOCUS       DAORPG010000048         5368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_921_length_5368_cov_3.552230, whole genome shotgun sequence.
ACCESSION   DAORPG010000048 DAORPG010000000
VERSION     DAORPG010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_921_length_5368_cov_3.552230"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="PLX85_01865"
     CDS             complement(<1..883)
                     /locus_tag="PLX85_01865"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRC61955.1"
                     /translation="MSPQAFRRAAVVGTTAWGTTLAILLARNGLDVTLLARTEAEATA
                     LEAAREHERRLPGERFPSALVVTADVRSLTRAELACFVVPAQTMADAVRAVGAHVAPD
                     ATVLSASKGIEIESCRRMSEILATELPGRAVAALSGPNLSREVAAGLPGTTVIASVDG
                     PLEALRGAFHRPTFRVYTSSDLVGVELGGALKNIVAIAAGMVDALGYGDNAKAAVMTR
                     GLAEMTRLGVAAGADPLTFQGLAGVGDLIATSYSRLSRNRRLGERLAQGASFRDVLEE
                     SGETAEGAATAPAALRLA"
     gene            complement(880..1506)
                     /gene="plsY"
                     /locus_tag="PLX85_01870"
     CDS             complement(880..1506)
                     /gene="plsY"
                     /locus_tag="PLX85_01870"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRC61956.1"
                     /translation="MSVIVVAVVGYLLGSVQSGLLVGRVLRGIDVREYGSGKTGFTNT
                     LRTVGLAGALVVVALDILKGAAPVLIGRLVFDDSLASAAGGVGAVVGHTWPVFAGFRG
                     GRGVATSFGAFAAVAPVAALLTLVIGGVVLGTTRYVSLMSITAMLAGFALLTVFVLAG
                     WTDPEYELFGVAVTLSVELNHLGNFKRLLRGTEPKIGQGGVRRTPGRA"
     gene            complement(1503..2849)
                     /gene="der"
                     /locus_tag="PLX85_01875"
     CDS             complement(1503..2849)
                     /gene="der"
                     /locus_tag="PLX85_01875"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRC61957.1"
                     /translation="MTDTANAAPLVAIIGRPNVGKSALFNRLTRTRRALVEEVAGTTR
                     DRIYGWFDWRGRHVRVVDTGGVEGPEADPFSALIREQVLEAIDEADAIVLVVDAAEGV
                     TAADEAIVDLLRRSTKPVLLVGNKADRREARLNLVDLQALGLGDPILISAYHGQGVAD
                     VMDQVLDVVPEANAPEREERLRIAVVGRPNVGKSSLVNAILGEPRVIVSDVPGTTRDA
                     IDTPFEFEGAPLLLVDTAGIRRRGKIERGVERHSVQRAEHAIDRADVVFLVIDQGEPS
                     VAQDTHIAGYVTEQGKGLVIVVNKWDLAEDRGDRHGFARSLDHRYHFVPWAPVQFTSA
                     LTGEGIRDLLELAVHIDQVRRRRVQTSELNLVVHRAMAEHGPPSVHGKRLKVMYATQA
                     ETEPPTFVFFVNDPRLLHFSYRRFLENRLRAAFGFEGTAIRMVFRRRSEDRHEVSA"
     gene            complement(2846..4120)
                     /locus_tag="PLX85_01880"
     CDS             complement(2846..4120)
                     /locus_tag="PLX85_01880"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HRC61958.1"
                     /translation="MAWFSSLGWEEARAIARDLWRRTRANFRRSPGLAGFSLLIGVVL
                     WLIVTDVENPTRIDTFPAQIPVEAVNVGPDLAVANASLPSVQVRVSAPQDAWDRLTVA
                     NFRAFVNLNDLDAREQLVPVQVEVSGVSRARVVDVTPGRIQVNLEPLETRQVPVTTRV
                     VGVPPRGYEPGRIEPDRSTVEVAGPQSLVSRVSAVVASVNVTGLTVGLAQNVTLVPVV
                     EGGGEIRGVTVRPETIRVSVAVIQSQLTRTLPVTAEVTGQPAAGYRVSGVKVTPTTVT
                     IEGRIDVLQALDSLALPGVDVGGQTTDVKTTVRPTLPEGVTIVPSGATVAVEVTITPV
                     AGSIALTLAPEVTNVRQGLLARVNPGSVSVIIDGPLPRLNTLPAGAVRATVDASNLGA
                     GTSDLRVNVVAPDGVFVRQVQPGSVSVTLAPP"
     gene            complement(4120..4959)
                     /gene="cdaA"
                     /locus_tag="PLX85_01885"
     CDS             complement(4120..4959)
                     /gene="cdaA"
                     /locus_tag="PLX85_01885"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRC61959.1"
                     /translation="MPDFSGAFGDAADRLTPSAVIDILIVSVSVYWLLLLIRGTTAMT
                     VLRGAVVLLGGAFLLSRALDLLVVNWLLRNSVTGIVIAMAIVFQPEIRRALERLGRTG
                     LRSSLRREERLHTVDTVVRAASRLARQGRGALIVLERETGLQEVIDTGVPLGADLSAE
                     LLGTIFVANSPLHDGAAIIRLDRVVAAGCTLPLSETPLPAEYGMRHRAALGITERTDA
                     VVVVVSEERGEVSLASNGRMLPDLDEVRLGRQLYRLFDLDGLGPEPLRLEGSGDNPER
                     QAS"
     gene            complement(5002..>5368)
                     /locus_tag="PLX85_01890"
     CDS             complement(5002..>5368)
                     /locus_tag="PLX85_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007305101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRC61960.1"
                     /translation="TEADIGRASELPGGGSVLQTDDAVAAVRGAAALYTDVWASMGEE
                     HLLAERIEVFRDYQLNGELLAAAPADALVMHDLPAHRGEEITDEAIESPRSIVFDQAE
                     NRLHAQQALLMLLMGRSPQ"
BASE COUNT          908 a         1939 c         1768 g          753 t
ORIGIN      
        1 ccgcgagccg cagcgcggcc ggggcggtcg cggcgccctc ggcggtctcg cccgactcct
       61 cgaggacatc ccggaacgaa gcgccctgcg cgaggcgctc gccgagccgt cggtttcgcg
      121 acagtcggct gtacgaagtg gcgatcaggt cgccgacgcc ggccagcccc tggaaggtca
      181 gcgggtcggc gcccgcggcg acgccgagac gcgtcatctc ggccagaccg cgcgtcatca
      241 ccgctgcctt cgcgttgtcg ccgtagccaa gcgcatcgac catgccggca gcaatcgcga
      301 cgatgttctt caatgcaccc ccgagctcaa ctccgacgag gtcactggag gtgtacaccc
      361 ggaacgtcgg gcggtgaaag gcgccacgca gagcctcgag cggcccgtca accgaggcga
      421 tgacggtcgt tcccggcagg cccgccgcca cttcgcgcga caggttgggt ccagagagcg
      481 cggcgacggc acggccgggg agttccgtcg ccagaatctc ggacatccga cggcacgact
      541 cgatctcgat acccttcgat gcgctcaaca cggtcgcgtc gggtgcgacg tgcgcaccga
      601 ccgcccggac ggcatcggcc atcgtctgcg ccggcaccac gaagcaggcc agttccgctc
      661 gcgtcagtga gcgcacatcc gcggtgacga ccagcgctga ggggaaacgc tcgccgggca
      721 gccgccgctc gtgctcacgc gcagcttcga gagcagtagc ttcggcctcg gtccgggcga
      781 gcagcgtgac gtcgaggccg ttgcgagcca gcaggatcgc gagcgtcgtg ccccaggccg
      841 tggtgccgac aaccgccgcc cggcggaacg cctgcgggct catgcccgcc cgggcgtccg
      901 ccggacgccg ccctgtccga tcttcggctc ggtaccgcgg agcagccgct tgaagttccc
      961 gaggtgattc agctccaccg acaacgtcac cgccacgccg aacagctcgt actcagggtc
     1021 ggtccagccg gcgaggacaa acacagtcag tagcgcgaag cccgcgagca tcgccgtgat
     1081 cgacatcagc gacacgtagc gcgtcgtacc gaggacgacc ccgccgatca ccagggtcag
     1141 gagcgccgcc accggtgcga ccgccgcgaa cgcgccgaac gaggtcgcga ctccccggcc
     1201 acctcggaag cccgcgaaca ccggccacgt gtgcccgact acggcgccaa ccccgccggc
     1261 tgccgaggcc agcgagtcat cgaagacgag ccgcccgatc agcaccgggg ccgccccctt
     1321 caggatgtcg agggcgacca caaccagggc gccggcgaga cccaccgtgc gcaatgtgtt
     1381 cgtgaatccg gtcttcccgg agccgtactc gcgcacatcg atgccacgta gcacacggcc
     1441 gacgaggagc ccggactgca ccgaacccag caagtacccg acgacggcga ccacgatcac
     1501 gctcacgcag acacctcgtg gcggtcctcc gagcgccgtc ggaacaccat gcggatcgcg
     1561 gtcccctcga acccgaacgc cgcccgcagc cgattctcca ggaagcggcg ataggagaag
     1621 tgcagcagcc gggggtcgtt gacgaagaat acgaacgtcg gcggctccgt ctccgcctgc
     1681 gtcgcataca tcaccttgag tcgcttgccg tggaccgagg gcgggccgtg ctcggccatt
     1741 gcccgatgca cgaccaggtt caactcggat gtctgcacgc gccgccggcg cacctggtcg
     1801 atgtgtaccg ccaactccaa caggtcgcgg atgccttctc ccgtcagcgc ggaggtgaac
     1861 tgcaccggcg cccagggcac gaagtgatag cggtggtcga ggctcctggc gaatccgtga
     1921 cgatcacccc ggtcctccgc cagatcccac ttgttcacga cgatcacgag gcctttgccc
     1981 tgctcggtca cgtaccccgc gatgtgcgtg tcctgcgcga ccgacggctc cccctggtcg
     2041 atcacgagga acacgacgtc cgcacggtcg atcgcgtgct ccgctcgttg cacggaatgg
     2101 cgctcgacgc cgcgctcgat cttgcctcgc cgccggatgc cggctgtgtc aacgaggagc
     2161 agcggcgccc cttcgaactc gaagggcgtg tcgatcgcat cgcgggtcgt gcccggcacg
     2221 tcggacacga tgacccgcgg ctcaccaagg atggcattca cgagcgacga cttgcctacg
     2281 ttgggtcggc ccacgaccgc gatccgcagt cgctcctccc gctccggggc gttcgcctcc
     2341 ggcaccacgt ccaggacctg gtccatgacg tccgcgacgc cttggccgtg gtacgccgaa
     2401 atcaggatcg ggtcccccaa cccgagcgcc tggaggtcga cgaggttcaa gcgcgcctcg
     2461 cgacggtcgg ccttgtttcc gacgaggagg accggcttcg tcgagcggcg caacaggtcg
     2521 acgatggcct cgtcggcggc tgtcacgccc tcggcggcat cgacgaccag cacgatggcg
     2581 tccgcctcgt cgatcgcctc gagcacctgt tcgcggatga gcgccgaaaa gggatcggcc
     2641 tcgggacctt cgacgccgcc cgtgtcgacg acacgcacgt ggcggccgcg ccagtcgaac
     2701 cagccgtaga tccggtcgcg tgtcgtgccc gccacctctt cgaccagggc gcgacgagtc
     2761 ctcgtcaggc ggttgaacag cgcggacttg cccacgttcg ggcgcccgat gattgcgacg
     2821 aggggagcgg cattggccgt atcggtcatg gtggagccag cgtaacggag acactcccgg
     2881 gctgcacctg gcgcacgaat acaccatcgg gcgcgacgac gttcacccgc aggtcgctgg
     2941 tccccgcacc gaggttcgat gcatcgaccg tcgcgcggac cgcgcctgcc ggcagcgtat
     3001 tcagacgcgg aagcggaccg tcgatgatca ccgagacgct tcccggattc actcgcgcga
     3061 gcaacccctg ccgcacgttg gtcacctcag gcgcgagcgt gagcgcgatc gagcccgcga
     3121 ccggcgttat cgtcacctcg acagcgacgg tcgccccgga aggtacgatt gtgacgcctt
     3181 ccggcagtgt gggccgcacg gtcgtcttga cgtcggtcgt ctgacccccg acatcgaccc
     3241 ccggcagggc gagggagtcg agcgcctgca gcacatcgat acgtccctcg atcgtcacgg
     3301 tcgtcggcgt caccttgacc cccgagactc ggtagcccgc ggctggctgc cccgtaacct
     3361 cggcggtgac cggtagcgtt cgtgtgagct gggactggat cacggccact gagacccgaa
     3421 tcgtctccgg ccgcaccgtc acgcctcgga tctccccgcc gccctcgacc acgggaacga
     3481 gcgtgacgtt ctgcgccagt cccaccgtca atccggtgac gttcaccgag gcgacgacgg
     3541 cactcacgcg cgaaaccagc gattgcgggc cggcgacctc gaccgtgctg cggtccggct
     3601 caattcgtcc cggctcgtat ccccgcggcg gcacgccgac gacccgcgtg gtgacgggca
     3661 cctgccgcgt ctcgagcggc tcaaggttga cctggatgcg gcccggggtg acgtcgacga
     3721 cgcgagcacg gctcacaccg ctcacctcga cctgcaccgg cacgagttgt tctcgcgcat
     3781 cgaggtcgtt gaggttcacg aaggcccgga aattcgccac ggtgagccgg tcccaggcgt
     3841 cctgcggcgc cgatacgcgt acctgcactg acggcaacga ggcgttcgcg accgcgaggt
     3901 ccgggccgac attgaccgct tcgacaggaa tctgcgccgg aaaggtatcg attcgggtcg
     3961 ggttctcgac gtcggtcacg atgagccaga gcacgacgcc gatgagcagg gagaagcccg
     4021 cgaggcctgg actgcgccgg aagttcgcgc gtgtgcgccg ccagaggtcg cgcgcaatcg
     4081 cccgcgcctc ctcccaccca agcgaggaga accacgccac tacgacgcct gccgttcggg
     4141 gttgtcgccg ctgccctcga ggcggagcgg ctccggaccg aggccatcga ggtcgaacag
     4201 gcggtacagc tggcggccga gccggacctc atcaaggtcg gggagcatgc gcccattcga
     4261 tgcgagcgag acctcgccgc gctcctcaga gacgacgacc acaacggcat ccgtccgctc
     4321 ggtgatgccg agcgcggccc ggtggcgcat cccgtactcg gcggggagtg gggtctcgga
     4381 cagcggcagg gtgcaacccg cggcgacgac gcggtcgagg cgaatgatcg ccgcaccgtc
     4441 gtgtaacgga gaattcgcga cgaaaatcgt gccgaggagt tcggcggaca ggtccgcgcc
     4501 cagcggcacc ccggtgtcga tcacctcctg cagcccggtc tcacgctcga gcacgatcag
     4561 cgccccccgg ccctgccttg cgaggcggct cgccgcccgc acgacggtat caaccgtgtg
     4621 cagccgctcc tcgcggcgga gtgaagagcg aagtccggtg cgccccagcc gctcgagggc
     4681 ccggcggatc tccggctgga agacgatagc catcgcgatc acgatgcccg tgaccgagtt
     4741 gcgcagcagc cagttcacga ccagcaagtc gagcgctcgg ctgagcagga aggcgccccc
     4801 gaggagcacc acggcgcccc gcagcaccgt catagccgtc gtgccccgga tgagcagcag
     4861 cagccagtac accgagaccg agacgatgag gatgtcgata acggccgaag gtgtcagtcg
     4921 gtcggcggcg tcaccgaagg caccggagaa atcgggcatc cgtgacgcct tccgcggggc
     4981 ccgcaccggc gcccgcccga attactgggg ggagcgtccc atcaagagca tcaacaacgc
     5041 ctgctgcgca tgcaaacggt tctccgcctg gtcgaacacg atcgagcgcg gagactcgat
     5101 cgcctcatcc gtgatctcct cgccgcggtg ggccggcagg tcgtgcatca cgagggcgtc
     5161 cgcgggcgcg gccgcgagca actccccgtt gagctggtag tcacggaaga cctcgatgcg
     5221 ctccgccagc aggtgttcct cgcccatgga tgcccagaca tccgtataca gggccgccgc
     5281 tccgcgaacg gccgcgacgg catcatcggt ctgcagtaca gatccgccac ccggcagctc
     5341 ggacgcccgc ccgatgtccg cctccgta
//
LOCUS       DAORPG010000049         5308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_945_length_5308_cov_4.050828, whole genome shotgun sequence.
ACCESSION   DAORPG010000049 DAORPG010000000
VERSION     DAORPG010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_945_length_5308_cov_4.050828"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..700)
                     /locus_tag="PLX85_01895"
     CDS             complement(86..700)
                     /locus_tag="PLX85_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61961.1"
                     /translation="MTLLLLPVAWLIVLVVNIAPAFMPPTWAVLATFHIATPELPLLP
                     LTVGGAAASAFGRSRLALLSRTTSRFLPAREVRNAVALGALFERHQRLGLLLLFAYCL
                     GPLPSNWLFIGAGLARIRLRVVAVTFFLSRALADTFWVWGAGRASASLATTFERYLTS
                     WQAILLQLAAMATVVLLLRLPWTRWLPIETAETAEVPRTDVRST"
     gene            complement(848..1144)
                     /locus_tag="PLX85_01900"
     CDS             complement(848..1144)
                     /locus_tag="PLX85_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61962.1"
                     /translation="MPLKITHRKEQEVPAPSASGKANEELYQLKMEMSKLAQGMVLEV
                     DAGSTKAIRGTKGLITRASKQLGRPFVHWHQGTKVFAKPAEAPRRRGRPRKVQP"
     gene            1688..2656
                     /locus_tag="PLX85_01905"
     CDS             1688..2656
                     /locus_tag="PLX85_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61963.1"
                     /translation="MPGGPRMTESSWMGGPVRVLSYLVTLPERALRAVFAGVGGTIHE
                     TAQLMLPRFVRRSRLYEATAKNALRIAVELVGDVPRDDRQPTEGDSARRVAVKKAAGN
                     VVEFGSIAAFGFSPLWLLAGAADVMNGSRVFLRALEDELAASGLLADGTHFGSVDQLI
                     GALEGTTGTTARAIDLPPLELSELKRSLAELRESVQTLPSPTDLMRLFNGLVATAAME
                     QRPLLEVSTGIGLAFLTSAKNVGREHVWAPYREDWEPLRNEGFASYARRVARPYRDAV
                     VSHFDGNRVTVTERLPRASSRLAAGGWKRLRGLRPRRGDASGRGAA"
     gene            2653..3717
                     /locus_tag="PLX85_01910"
     CDS             2653..3717
                     /locus_tag="PLX85_01910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013918998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRC61964.1"
                     /translation="MSDGLPLRLGILDQSPIRRGGTPREALLATIELATAADRLGYSR
                     YWVAEHHNTPTLASASPELLIPLIASKTERIRVGSGGVMLTHYSSLKVAETFRMLEAL
                     FPGRIDMGLGRAPGSDRRTAAALQHGPGALPLEAYPEQVRDVLRYMLDEVPADHPFHG
                     IRAVPAGPSMPAPWLLGSAYDSARFAAEQGLPFSFAHFISPMGGTKVVREYREQFRPS
                     ALCPEPLVNVGVAALCAETDADAHRIGLSRHLMRLRREQGRASGGVPSIEEALLTDFT
                     EPEIDYIRYQQSLTFEGRPQRVAAGLRELAEEYEADELLVVTITHDYADRLRSYELLA
                     EAMGLAPPGEAVSTQLQGGA"
     gene            complement(3719..4192)
                     /locus_tag="PLX85_01915"
     CDS             complement(3719..4192)
                     /locus_tag="PLX85_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61965.1"
                     /translation="MIDGGYDIDLEAVNQNVEEAEVVTFYFPMLRRTLLVDMRCGPLE
                     GPLICVVAMVDSSAQRYESLRRLRPQLPRPESLTMIPWAHSIGALEETGVWDRVTGRL
                     LECGAADVLVDAGRCLRELRILELNELRLAIRGPEYRTVWGRAGVGDGAEPPAAV"
     gene            complement(4189..>5308)
                     /gene="tyrS"
                     /locus_tag="PLX85_01920"
     CDS             complement(4189..>5308)
                     /gene="tyrS"
                     /locus_tag="PLX85_01920"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRC61966.1"
                     /translation="LRARLEEDRPLSVYCGYDPTSVDLTLGHTLTMRKLRQFQDFGHN
                     VTFLIGNFTGLVGDASDKDKRRPMLTPEQLAANAATYEKQAGRILDLSRTTIKYNADW
                     LGALTFADVVRLCSHFTVAEFLKRDNFAKRMERGDAIHLHEFMYAIMQAYDAVALKTD
                     VQVGGTEQLFNLMAGRKLQEEFDQRPQIVLMVPILVGTDGHQRMSKSTGNYIGVDEAP
                     NDMYGKVMSLPDVAMRDYFTLLTDLPADGVESQLAGPPIEAKKRLAYEITALMYDLEA
                     AAAAQTYFESTFQRGETPEEMQEFTIDGEAEARLDRVLRTAGLAASAAEVRRLVAQGA
                     VRVNGNAVTDFAFVLHGGDEVRVGRHRFLRMVEAGANT"
BASE COUNT          856 a         1611 c         1945 g          896 t
ORIGIN      
        1 gtgtggctga ctcctgagat cgtgtggtgg ggtgacggcg caccgcccgc gccatttgcg
       61 ggtgcacccg agggctgaac gcgtctcagg tcgaacggac gtcagtgcgg ggtacctccg
      121 cggtctctgc ggtctcgatg ggcaaccaac gggtccaggg caggcgcagg agcagcacga
      181 cggtggccat cgctgcgagc tgcaggagga tcgcctgcca gctggtgagg taacgctcga
      241 aagtcgtggc gagcgaggcg gaggcgcggc cggcgcccca gacccagaag gtgtccgcca
      301 gagcgcggct caagaagaag gtcacggcca cgacgcgcag ccggatgcgc gcgagtccgg
      361 ctccgatgaa cagccagttg ctggggagcg gcccgaggca gtaggcgaac aggagcagca
      421 agccgaggcg ttgatgacgc tcgaagagcg cgccgagcgc gaccgcgttt cggacctcgc
      481 gggcgggcag gaacctcgat gtggtccggc tgagcagagc gagacgtgaa cgaccgaacg
      541 cggaggcggc ggcgccaccg acggtgagtg ggaggagcgg gagctcgggc gtggcgatgt
      601 ggaaggtggc gaggaccgcc caggttggtg gcatgaaggc cggggcgatg ttcacgacga
      661 ggacgatgag ccacgcgacc gggagcagca gcagcgtcat cggcaccgtg cccgctcgag
      721 tctgcgcgtt cgagcccgcg gcgtgtcaga tagcgaaggc ggaacgccgt ctcacgcgag
      781 caggccggtt tcgccggcct gctcgcgtga ttgtttcagt ggtgggctcg atcgagccga
      841 tgtgcgccta gggctgcact ttccgagggc gaccgcggcg acgaggcgcc tcggcgggct
      901 tggcgaagac tttggtgccc tggtgccagt ggacgaaggg gcgcccgagc tgcttgctgg
      961 cgcgggtgat gaggcccttg gtgcctcgga tggccttggt gcttcccgca tccacttcga
     1021 ggaccatgcc ctgcgccagc ttggacattt ccatcttcag ctgatacagc tcctcgttgg
     1081 ctttcccgct tgcagagggg gcggggactt cctgctcttt gcggtgagtg atcttcagcg
     1141 gcatggtaac ccttctcttg agattgccca caaggtttac acccggaggc gtgaaccgca
     1201 caagtcagag accgctagat tagcgccaga tattcatcaa tttatggtcg ggttcgtttc
     1261 ttcgatgacg acgggacagg tcggaagttc cccggcgagc agtccgcgcg agtatgatca
     1321 aggacggacg gatgcgatga cgaaggtgtg cgaggaagcg gcgaatgcct gatgtccgga
     1381 ttgtcgcgaa ggcgatgaac gacctggcgg atgactggca ctgggtaacg cttgtggagg
     1441 cgaccgagct ggaacctcgg gtgttccgcg ctctgcaggt gcggactcgg tgcggactga
     1501 tgatctcggc tactccgcgt gtagtgcaat tgttcgacat gggtgcgccg gatactccga
     1561 cctgtcctcg ttgcctggat gacgggctga acccggcgct ccgcgcggcg atttctgagg
     1621 tgatcgagtc ttctgagcgc atctccgagc ctgtgggcct ggccgaacga acagggggga
     1681 gcgcccgatg ccgggcggcc ctcggatgac tgagtcgagc tggatgggcg gtcccgtccg
     1741 cgtgctgagc tacctcgtca cgttgccgga gcgggcgctg cgcgcggtgt ttgccggcgt
     1801 gggcgggacc attcacgaga cagcgcagct gatgttgccc cggttcgtgc ggcggtcgcg
     1861 gctctacgaa gcgaccgcga agaacgcgct ccgaatcgcc gtcgaactcg tcggcgatgt
     1921 cccccgtgat gaccgccagc cgacggaggg tgacagcgca cgccgggttg cggtgaagaa
     1981 ggcggccggg aacgtggtcg aattcgggtc gatcgcggcg ttcggattct cgccgttgtg
     2041 gctgctcgcg ggtgcggcag atgtgatgaa cgggtcgcga gtattcctgc gcgctctgga
     2101 ggatgaactg gcggcgagcg ggctgctggc cgacgggacg cacttcgggt cagtggacca
     2161 gctgatcggc gcactcgaag ggacgacagg gacgacggca cgtgcgatcg acctgccgcc
     2221 gctggagctt tctgagttga aacggtcgct tgcggagctg cgggaaagcg tgcagacgct
     2281 cccgtcgccg acggacttga tgaggctgtt caacggactg gtcgccacgg cggcgatgga
     2341 gcaacgcccg ctcctcgagg tgtccaccgg catcggactc gcgtttctga cgtcggcgaa
     2401 aaacgtgggc cgcgaacatg tgtgggcgcc gtaccgcgag gactgggaac cactgcggaa
     2461 cgagggtttc gcctcgtacg cgcgtcgggt ggcacggccc tatcgtgatg ccgtggtgtc
     2521 acattttgac ggcaaccgag tcacggttac ggaacgcctg ccgcgtgcca gctctcgcct
     2581 cgcagcgggc gggtggaagc gtctgcgcgg cctgcggccg cggcgtggcg acgcgtccgg
     2641 ccgaggtgcg gcatgagcga cgggctgccc ctacgcctcg ggattctcga ccagtcgccg
     2701 atccggcgcg gggggacgcc tcgcgaggcg ttgctcgcga cgatcgagtt ggcgacggcg
     2761 gccgaccgcc tgggttactc gcgatactgg gtggcggagc accacaacac gccgacgctt
     2821 gcgagcgcgt cgccggagct gctgatcccg ctgatcgcct cgaagacgga acgcatccgc
     2881 gtcggttcgg gcggcgtgat gctgacccac tacagcagcc tgaaggtcgc agagacgttt
     2941 cggatgttgg aggccctgtt tccgggccgc atcgacatgg ggctggggcg ggcacccggc
     3001 agcgaccggc gaacggcagc ggcgttgcag cacgggccgg gagcgttgcc tctggaggcg
     3061 tatcccgagc aggtgcgtga cgtgctgcga tacatgctcg acgaggtgcc ggcggaccac
     3121 ccgttccacg gaattcgggc ggtgcccgcg gggccctcga tgccggcgcc ctggttgctc
     3181 ggttcggcgt acgacagcgc tcgctttgct gccgagcagg gattgccgtt ttcgttcgcg
     3241 cacttcatca gcccgatggg cgggacgaag gtcgtgcgcg agtaccgtga gcagttccgg
     3301 ccctcggcac tgtgcccgga gccgctggtg aacgtgggag tcgcggcgct gtgcgccgag
     3361 acggacgcgg acgctcaccg aatcgggctg agccgccacc tcatgcgcct gcgccgcgag
     3421 cagggccgtg cctcgggggg cgtcccgagc atcgaggagg ccctgctaac tgatttcacc
     3481 gagccggaga tcgactacat ccggtaccag cagagcctga cgttcgaggg cagaccacag
     3541 cgtgtggccg ccgggttacg cgagctggcc gaggagtacg aagcggacga gctgttggtg
     3601 gtaacaatca cgcacgacta cgcggaccgg ctgcggtcgt acgagttgct cgccgaagcg
     3661 atggggctcg ctcctccggg tgaggccgtg tcgacccagc tgcaaggcgg tgcctgagtc
     3721 agacggcggc gggcggctca gcgccgtcgc cgacgccggc gcggccccag acggtgcggt
     3781 attcggggcc gcggatggcc agccggagtt cgttcagctc gaggatgcgc agctcgcgga
     3841 gacagcgacc agcatcgacg aggacgtcgg cggctccgca ttcgagcagg cgacccgtga
     3901 cgcgatccca gacgcctgtc tcctcgagag ctccgatact gtgcgcccag ggaatcatcg
     3961 tgagggactc ggggcgtggg agctgcgggc ggagccgtcg cagcgactcg tagcgctggg
     4021 cggagctatc gaccatggcg acgacacaaa taagcgggcc ttcgaggggc ccgcagcgca
     4081 tgtccaccag cagcgtgcgg cgaagcatcg ggaagtagaa ggtgactacc tccgcctcct
     4141 cgacgttctg gttcaccgcc tcaaggtcga tgtcatatcc gccgtcgatc acgtgtttgc
     4201 ccccgcctcg accatgcgca ggaagcgatg gcgtcccacg cgtacctcgt cccccccgtg
     4261 gagcacgaaa gcgaagtcgg tgacggcatt gccgttcacg cgcaccgctc cctgcgccac
     4321 cagccgacgc acctccgccg cactggcggc gaggcccgca gtccgtagca cgcgatcgag
     4381 gcgggcttcg gcctcgccgt cgatcgtgaa ctcctgcatc tcttccggcg tttcgccacg
     4441 ctggaaggtc gactcgaagt aggtctgggc tgccgcagca gcctcgaggt cgtacatcaa
     4501 ggcggtgatc tcgtacgcga gccgcttctt cgcctcgatg ggtggccccg ccaactgcga
     4561 ctctacccca tcggcgggga gatcggtgag cagcgtgaag tagtcgcgca tggcgacatc
     4621 cggcagggac atgaccttgc cgtacatatc gttcggcgct tcgtcgacac cgatatagtt
     4681 gccggtcgac ttcgacatcc gctggtgccc atcggtgccc acgaggatgg gcaccatcag
     4741 gacgatctgt ggacgctggt cgaactcctc ctggagcttg cggccggcca tgaggttgaa
     4801 cagctgctcg gtgccgccga cctggacgtc cgttttgagg gcgaccgcat cgtaggcctg
     4861 catgatcgcg tacatgaact cgtgcaggtg gatggcgtcg ccccgctcca tccgcttcgc
     4921 gaagttgtcg cgcttcagga actcagccac ggtgaagtgg gagcagaggc ggacgacgtc
     4981 ggcgaacgtc agggcgccga gccaatcggc gttgtacttg atcgtcgtgc gggagaggtc
     5041 aaggatgcgg ccggcctgct tctcgtaggt ggcggcgttg gcggcgagct gctcgggcgt
     5101 gagcatcggc cgccgcttgt ccttgtctga ggcgtcgccg acgaggccgg tgaagttccc
     5161 gatgaggaac gtgacgttgt ggccgaagtc ctggaactgg cgcagcttgc gcatggtcag
     5221 ggtgtggcca agggtgaggt cgaccgaggt ggggtcgtac ccgcagtaga cgctcagcgg
     5281 ccgatcttcc tcgaggcgag cgcggagc
//
LOCUS       DAORPG010000050         5301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_952_length_5301_cov_3.577202, whole genome shotgun sequence.
ACCESSION   DAORPG010000050 DAORPG010000000
VERSION     DAORPG010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_952_length_5301_cov_3.577202"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            342..416
                     /locus_tag="PLX85_01925"
     tRNA            342..416
                     /locus_tag="PLX85_01925"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:375..377,aa:Arg,seq:ccg)
     gene            515..1000
                     /locus_tag="PLX85_01930"
     CDS             515..1000
                     /locus_tag="PLX85_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61967.1"
                     /translation="MNRYDESAYAARWMLVIVALTMIVCLASAFVVLARDAPWWVPVQ
                     LVAVGVILGFVEFLFMRLRIEVTEARVRFRFGPFGPTLKRSEIRSAEVVPYRWVAFGG
                     WGIRIGRVAGRFMRAYSVPFLRTGVAIETNAGARYYVSSRRPELFAAAILAGRSREGS
                     A"
     gene            997..1830
                     /locus_tag="PLX85_01935"
     CDS             997..1830
                     /locus_tag="PLX85_01935"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRC61968.1"
                     /translation="MSDERRRQQRRERQRRRTTSRPPLQQAPAGPMPLPGVFGWVQRN
                     GRLFALLGVIVLLISLGGRFLTSNRSNNSSSATTTPTPKASATPTASPTATPTFGPTG
                     TPSADGVYRTYTSAPPMQINENGKYEALIRTEKGDIRIELLPQSAPEYVNNFVFLARN
                     HFYDGLTFHRVVAGFAAQAGDPLGNNQGGPGYTLDPELNSIPFDEGIISMAGGQQGVS
                     GSQFFITMSPQPALKDNGFSAFGRVIEGIDVVKSLTVRDPGKPAQPPGDRILSVQITE
                     R"
     gene            complement(1848..2066)
                     /locus_tag="PLX85_01940"
     CDS             complement(1848..2066)
                     /locus_tag="PLX85_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61969.1"
                     /translation="MPEGVPAYILVVRIATILGMAFSIAIGLLLLIGGFVLPSLVAFA
                     LFVPSIATMVYAEKRAALAQTNARQRPS"
     gene            complement(2111..2187)
                     /locus_tag="PLX85_01945"
     tRNA            complement(2111..2187)
                     /locus_tag="PLX85_01945"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2151..2153),aa:Pro,seq:tgg)
     gene            complement(2249..2440)
                     /locus_tag="PLX85_01950"
     CDS             complement(2249..2440)
                     /locus_tag="PLX85_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61970.1"
                     /translation="MTDVRLWLGRTLALVVALATGAVWFVAASRFVYRVGPHEAAVAL
                     GLLTGIAVALTMWRWTVTR"
     gene            complement(2433..3371)
                     /locus_tag="PLX85_01955"
     CDS             complement(2433..3371)
                     /locus_tag="PLX85_01955"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HRC61971.1"
                     /translation="MAHVDLSVTLAAGQKQELRLRNPVMVASGTFSNGIEFAKRFDVD
                     ELGAIVSKGTTLRPRRGNPVPRTVETPSGMINSIGFQNVGVGTLVSEVAPVWARWQAP
                     VLVNIMGETLAEYGQLAQRLDGVPGVAGLEVNISCPNVEAGGLEFGQDPLLAADVTRE
                     VRRHTALPMSVKVTPGVTDLRPIVAAVSDAGADAITVMNTVPAMAIDVRARRPVLATA
                     FGGLSGPAVKPIALRLVYLAASVTNLPILAAGGVSSGLDAVEFLMAGASAIQVGTATF
                     RDPQAPWKVLAELREWCEREGVSSIGEIVGVARRRD"
     gene            complement(3495..3746)
                     /locus_tag="PLX85_01960"
     CDS             complement(3495..3746)
                     /locus_tag="PLX85_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61972.1"
                     /translation="MTSEARERTTPVSNCVHHWIIEAPSGRESVGRCKRCNATKNFAN
                     STESVMWEQTNTLRNPLRSGVRISKPSEILLSDEMGEDA"
     gene            4102..5142
                     /locus_tag="PLX85_01965"
     CDS             4102..5142
                     /locus_tag="PLX85_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015201971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRC61973.1"
                     /translation="MADQQTVLAALQSVRDPELHRDIVSLNMVRDLEVEGSRVAFNLV
                     LTTPACPLRETIDRDVRAALAVLPDITEVDIHWDAEVRGGPMREGGPKPVEGVKNVIA
                     VGSNKGGVGKSTVSVNLAVALAQLGARVGLLDADITGPNIPTMMGIEAGGLAEGTGGL
                     HTIEAHGVRCISIGFVLPPGTPVVWRGPMIGSGVRQLLHDVDWGELDYLIVDLPPGTS
                     DASMSLAQEAEIAGVVIVSMPQKVSVEDATKAVGMFDRMGVPIFGIVENMSSDIFGRG
                     GAQEAADELGIEFLGEIELDAGVRQGADTGVPIVAGAPDSPQARAFRHLAEQVAARCS
                     VLRWSTDIGAVA"
BASE COUNT          891 a         1830 c         1709 g          871 t
ORIGIN      
        1 caacgcgacc gcctcgcgca gggtgatccc gcggcggacg ccccgcacgg cggcctcggc
       61 ggtcacttcc gcgacgcgca gcccgctctc gtccgcgagc gcgacggcgc gactacgtag
      121 ctcaggctgc tcagccagtc gttgctcgag cgcaagcgcc ggcacggtaa gacacgcgta
      181 cagcacttca accccccgct acggcggtag ggtagcacac tagttctagt gccctgaggg
      241 tacggcggca tcacggacac cgaggcgaag agcggaggac gctgctacta tgaatccgcg
      301 gctgagatcg cgatcgcgaa ttcgtggcca gcagaatcgt tgccctcgta gctcagtgga
      361 tagagcaccg gcctccggag ccgggggcgc aggttcgagt cctgccgagg gtaccagtct
      421 cccccttacg tctttgggct ggaaaacagc atctccatgt gacacgctgg atctcgatgc
      481 cgttgcaagg cgctcccgcc ggggactcac cggcttgaac cgctacgacg aatccgccta
      541 cgccgcgcgc tggatgctgg tcatcgtggc gctcaccatg atcgtgtgcc tcgccagcgc
      601 gttcgtcgtc ctggcacggg atgctccgtg gtgggtgcca gtgcagctcg tcgccgtagg
      661 cgtgatcctc ggcttcgtcg agttcctgtt tatgcgactc cgtatcgaag tgaccgaggc
      721 gcgagtgcgc ttccgcttcg gcccgtttgg ccccaccctg aagcggtcgg agatccgatc
      781 cgccgaggtc gtgccctacc gctgggtggc ctttgggggc tggggcatcc gcattggccg
      841 cgtcgccgga cgcttcatgc gcgcctactc cgtgccattc ctgcgcacgg gagtcgcgat
      901 tgagacaaac gccggcgcgc gttactacgt atcgagtcga cggcctgagt tgttcgcagc
      961 cgcgatcctc gcaggtcgat cccgggaagg ttccgcatga gcgacgaacg caggcgacag
     1021 cagcgacgcg agcgacagcg tcgacggacc acgagccgcc cgccgctgca gcaggcgccc
     1081 gcggggccga tgccgctgcc cggcgtcttc ggctgggtgc aacgcaacgg acgcctgttc
     1141 gcgttgctcg gcgtgatcgt cctcctgatt agcctcggtg gacgtttcct gaccagcaac
     1201 cgctcgaaca acagctcgag cgccaccacg acccctactc cgaaggcaag cgccacgccc
     1261 acggccagcc ccaccgccac gccgaccttc ggtcccaccg gcaccccctc ggccgacggc
     1321 gtctaccgca cctacacctc ggcgcccccg atgcagatca acgagaacgg caagtacgaa
     1381 gcgttgatcc gcacggagaa gggcgacatt cgcatcgagc tgcttccgca atcggcgccg
     1441 gagtacgtga ataacttcgt gttcctggct cgcaaccact tctacgacgg gctgaccttc
     1501 caccgggtcg tagccgggtt cgcggcccag gccggagacc cgctcggcaa taaccagggt
     1561 ggtccgggct ataccctcga cccagagctg aacagcatcc cgttcgatga aggcatcatc
     1621 tccatggccg gtggtcagca gggtgtaagc gggtcgcagt tcttcatcac catgagtccc
     1681 cagccggcac tgaaggacaa cggcttcagc gccttcgggc gggtcatcga gggcatcgat
     1741 gtagtgaagt cgttgactgt ccgcgaccct ggcaagcccg ctcagccccc cggtgaccgc
     1801 atcctgtcgg tgcagatcac cgagcgctga cgagggatca acgcgggcta gctcggccgc
     1861 tggcgcgcgt tcgtctgcgc cagcgcggcg cgcttttcgg cgtaaaccat cgtcgcaatc
     1921 gagggcacga acagcgcgaa cgcgaccaac gacggcagca cgaatccgcc aatgaggagc
     1981 agtagcccga tggcgatcga aaacgccatc ccgaggatcg tcgcgatccg tacgacgagg
     2041 atgtaagccg gcacgccctc gggcatcgtg ttcctttgtg agcaacgggc cgctgatcgc
     2101 ccggggacgg tggtcggggt gcacagattc gaactgtggg cctctgcgtc ccaaacgcag
     2161 cgctctacca ggctgagcta caccccgctc ctccgacgct acgcgcgacc gcgtcgcagc
     2221 gtcaaactcg caacgcgtct tctcgaggtc agcgtgtgac cgtccatcgc cacatggtga
     2281 gtgcgaccgc gatgccggtg agcagcccga gggccaccgc cgcctcgtgc gggccgacgc
     2341 gatagacgaa ccgcgaggcg gcgacgaacc atacggcgcc ggtcgccagc gccacgacca
     2401 acgcgagtgt gcgcccgagc cacaaccgca cgtcagtcac gccgtcgcgc cacgccgaca
     2461 atctcaccga tggatgagac accctcgcgc tcgcaccact cgcggagttc ggcgagtacc
     2521 ttccagggcg cttgcgggtc gcggaacgta gccgtcccca cctgtatcgc ggacgcgccg
     2581 gccatcagga actcgaccgc gtcgaggccc gacgacacgc caccagccgc cagaatcggg
     2641 agattggtca ccgacgccgc gaggtacacg agccgtagcg caatcggctt cactgcgggg
     2701 ccggaaagcc ctccgaacgc cgtcgccagg acggggcgac gtgcgcgaac atcgattgcc
     2761 atcgcaggca cggtgttcat cacggtaatc gcgtccgcgc cagcatccga gacggccgcg
     2821 acgatcgggc gcaggtcggt gacgcccggc gttaccttta cgctcatcgg cagtgccgta
     2881 tggcgacgca cctcacgcgt gacgtcggca gccagcaatg gatcttgtcc gaactccagc
     2941 ccaccggctt cgacgttcgg gcacgagatg ttcacctcga ggcccgccac gcccggcacg
     3001 ccgtccagtc gctgtgcgag ctgcccgtac tcggccaggg tctcgcccat gatgttcacg
     3061 aggacaggcg cctgccagcg cgcccatacc ggcgcgacct cgctcacgag cgtgccgacg
     3121 ccgacgttct ggaagccgat cgagttgatc atcccgctcg gcgtctcgac ggtgcgaggg
     3181 actgggttcc cccgccgcgg ccgcagcgtc gtccccttcg agacaatcgc gccgagctcg
     3241 tcgacatcga agcgcttcgc gaactcgatg ccgttcgaga acgtccccga ggccaccatg
     3301 accgggttcc tgagccgcag ctcctgcttc tgcccggctg cgagggtgac agaaaggtcg
     3361 acgtgcgcca tcagtgggac caccgtcgcg tcatgccccg atgatacgtc gtccgcgtgg
     3421 aacgggccgg gagtcgaaac gaaggaggcg ctcccatcgg gagcgcctcc tcaaatgatc
     3481 gccgcgcggg ggagctaggc gtcctcgccc atctcgtcgg agagcagaat ctcagagggc
     3541 ttcgagatcc gtacgccgct gcggagcggg ttgcgcagcg tgttggtctg ctcccacatg
     3601 acactctcgg tcgagtttgc gaagttcttg gtggcgttgc accgcttgca gcgccccacg
     3661 ctttcgcgcc cgctgggagc ctcaatgatc cagtggtgca cgcagttgct caccggagtg
     3721 gtgcgctcgc gtgcttcgct cgtcataatt acacctcctg cagcgatccg cgctccccgc
     3781 gtaggccgac cgctgtgctc tcctcgtcgt ctgggtctcg tcgagccgtt gcccttgaac
     3841 ccacttccga cacgtgtcac tctaccgggg actttgtgtc aggtcaagca ttttttcatt
     3901 aaatagttgt aaagtgagat aagagtccga gcggctgtcc gaagaggcgg gtatactggc
     3961 tctcggtggc cacaactcca agatgttccc ggcgcgcgcc gtccgagcgc gactccgccc
     4021 tcgaagtgct gagacctata gaacgccggg gagcctggtt tggttccctc atactcgtcg
     4081 tgcggtcatt acggaggaca catggctgat cagcagaccg ttctggccgc gttgcagagc
     4141 gttcgcgacc ctgaactcca ccgtgacatc gtttcgctga acatggttcg agacctcgag
     4201 gtggagggca gccgagtcgc cttcaacctc gttctcacca ccccggcctg cccgttgcgc
     4261 gagaccatcg accgcgatgt ccgtgcggcg ctcgcggtcc tgcccgacat cacggaggtg
     4321 gacatccact gggacgccga agtgcgcggc ggcccgatgc gtgagggtgg ccccaagccg
     4381 gtcgagggcg tgaaaaacgt catcgccgtc gggtccaaca agggcggcgt cggcaagtcg
     4441 accgtctccg tgaacctggc ggtcgcgctc gcgcagctcg gcgcgcgggt cgggcttctc
     4501 gacgccgaca tcacgggtcc gaacatcccc acgatgatgg gcatcgaggc cggcggcctc
     4561 gctgagggca cgggtggcct ccacaccatc gaggcacatg gtgtccgctg catctccatc
     4621 gggttcgtcc tgccgccggg gacgccagtc gtctggcgag gccccatgat cggctccggc
     4681 gtccgccagc tgctccacga cgtcgactgg ggcgagctcg actacctgat cgtcgacctc
     4741 ccgccgggca cctcggacgc ctcgatgtcg ctggcgcagg aggccgagat cgccggcgtc
     4801 gtcatcgtct cgatgccgca gaaagtgtcc gtcgaggacg ccacgaaggc cgtcggcatg
     4861 ttcgaccgca tgggcgtgcc catcttcggc atcgtcgaga acatgtcgtc ggacatcttc
     4921 ggccgcggtg gcgcgcagga agcggccgac gagctgggca tcgagttcct cggcgagatt
     4981 gagctggacg ccggcgtccg ccagggcgcc gacaccggcg tccccatcgt cgctggcgcg
     5041 cccgattcgc cgcaggcgcg agccttccgt cacctcgccg agcaggtggc ggctcgctgc
     5101 tcggtcttgc gctggtccac cgacatcggc gcggtggcgt aaccaggaag aaggacgagc
     5161 gagtggtcga cgaacaggct cagggccagg tagacgatcg aggcgaggac ggacgccgtc
     5221 gccgaggctc acgaggcggc cgcggccgtg gctctcgtag cgagactgaa gccccgcgcg
     5281 ccgcgacgcc gcagctcgac g
//
LOCUS       DAORPG010000051         5278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_959_length_5278_cov_3.535707, whole genome shotgun sequence.
ACCESSION   DAORPG010000051 DAORPG010000000
VERSION     DAORPG010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_959_length_5278_cov_3.535707"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="PLX85_01970"
     CDS             complement(<1..1195)
                     /locus_tag="PLX85_01970"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRC61974.1"
                     /translation="MLPMDDVRIVDLTQYAMGPFATRVLADYGADVIKIERPGGDPAR
                     QLGPFPKDVPHIERSGAFLALNTSKRSVVVDLKRPEGRDLVLGLVERAEIVVESFAPG
                     TLDHLGLGYDRLRAVNPRIVLTSISNFGQTGPYRDWKGTDLTLYAMGGPMISQGDPSR
                     EPLKTGGRAPGYQVGYAAALASAVGLTAAERRGHGEHLDVSAFEVLAHSIDSRLGRLL
                     GYQYNGRLVGRAGSGLGVGSGTYPCLDGFVLITSGAPRLPLTIRMIGREDLLDQPEWA
                     TLEARSHPDRVPEFDAYLLMWTLEHTKEEIRAACQQYGVLGGPLNTTADLFVDRNFAA
                     RGFFQTIDHPETGPLTYPGFQARFNTEPPQPPRKRAPLLGEHTVEVIRDVLDMQPADI
                     DRLRGA"
     gene            1481..1765
                     /locus_tag="PLX85_01975"
     CDS             1481..1765
                     /locus_tag="PLX85_01975"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HRC61975.1"
                     /translation="MSVYFVIQEEIHDPEALVEYREKARNSPHGGKLLAMDDAPIVLE
                     GNWHGGRALIIEFDDEAAFRAWYESPAYQEALKIRLAATDSRSALLHGMP"
     gene            complement(1906..2145)
                     /locus_tag="PLX85_01980"
     CDS             complement(1906..2145)
                     /locus_tag="PLX85_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61976.1"
                     /translation="MPDDSPPGGAPANEEFIADPTGHLLAIIDDADAAVAAERDLDGS
                     GIEGVRIFRGSRSADAIRRCRTVSLRSSIVMARVG"
     gene            complement(2169..2633)
                     /locus_tag="PLX85_01985"
     CDS             complement(2169..2633)
                     /locus_tag="PLX85_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61977.1"
                     /translation="MDNQWADPEGFVERPPAVRYRIRTPRGKVEEHRTVPTQDPHIFD
                     RWFHALAPQDQLFADRYADLLQQAASYFYASDGVYRPLEGIPDVPVSDEEVRERIRRI
                     DGGENSREATRYRGSAEARYPFGDLPFDQQRALIEQIEFLKREWEFELLGVD"
     gene            complement(2682..3167)
                     /locus_tag="PLX85_01990"
     CDS             complement(2682..3167)
                     /locus_tag="PLX85_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61978.1"
                     /translation="MEDELNRVQLGAVLWLAPDERQWLVQTLSLPRSREAREQLSGES
                     DPQAAIARRFRAVLMALFLDGADEEIPLALTADELWLLDVHLMQYDLRDSRLPSGRLL
                     AEFARKLWTTLTEVHRSELPRSLQKGRNDARENGDTDEGADAIAEVEALLRPAEDAGA
                     G"
     gene            complement(3241..3438)
                     /locus_tag="PLX85_01995"
     CDS             complement(3241..3438)
                     /locus_tag="PLX85_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015129841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HRC61979.1"
                     /translation="MPEVNPINVQKFLGGIDYPVDKQQLVEHAQEQQADDEVRHALER
                     PPMDRFNSPNDLSEAIGKLRP"
     gene            complement(3573..4769)
                     /locus_tag="PLX85_02000"
     CDS             complement(3573..4769)
                     /locus_tag="PLX85_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRC61980.1"
                     /translation="MLGVSAVVGAAWFAGLTSADAHDGTLAAAATLPGLPAVSNMTLA
                     DVTPAAELASNPPSRPAATVPSATPSPAAAELPSWVTAALAATQPAPSQSAATPGAPT
                     ATLAPEGSASDQRQTALQNQATPTPTASPTPAAPPPLYQTYTVRAGDTVSGIAARSGV
                     RTQDIINNNVGVISAGSLLLVGARLQIPTVPGVLHNISVGETLADIAEHYGVTVDDIV
                     GFPGNRIDDPSNVLEGTQILVVNGTPPVVQAAPAAATPEATPSAEAASTPEPTPVAPP
                     KPVYQFIWPLVGNITSYFGPAHPLGIDISAAFQPVAASASGKVVFVGGDPCCSYGYYI
                     DIQHADGYMTRYGHLSKFLVSLGQQVSQGDTIAISGNTGYSTGAHLHFEIRKNGAVRN
                     PLDFLP"
BASE COUNT          837 a         1683 c         1888 g          870 t
ORIGIN      
        1 cggcaccgcg cagccggtcg atatcagccg gttgcatatc gagcacgtcg cggatcacct
       61 cgacggtgtg ttcgccgagc agcggcgccc gtttgcgagg cggttgcggc ggctccgtgt
      121 tgaaccgcgc ctggaatccc gggtaggtga gcggacccgt ttctggatgg tcgatcgtct
      181 ggaagaagcc gcgcgccgcg aagttacggt cgacgaagag gtccgcggtc gtgttcagcg
      241 gcccgccgag gacgccgtac tgctggcacg cagcgcggat ttcttccttg gtgtgctcga
      301 gcgtccacat cagcaggtag gcgtcgaact ccgggacacg gtccgggtgg gaacgtgcct
      361 ccagcgtcgc ccattccggc tgatcgagaa gatcctcgcg tccgatcatg cggatggtga
      421 gcggcaatcg cggcgcgccg ctcgtgatga ggacgaagcc gtcgaggcag gggtaggtac
      481 ccgagccgac cccgagtcca ctgccagccc gcccaacgag acgcccgttg tactggtagc
      541 cgagcagccg gcctaggcgc gagtcgatcg agtgcgcgag cacctcgaaa gctgagacat
      601 cgaggtgttc gccgtgcccg cgccgctccg cggcggtgag gccgacggcg cttgcgagcg
      661 ccgcggcgta gcccacctga tagcccggcg cgcggccacc tgtcttgagc ggctctcgac
      721 tcggatcgcc ctgcgagatc atcggaccac ccatggcgta cagcgtgaga tcggtgccct
      781 tccaatcgcg atagggcccg gtctgcccga agttgctgat cgacgtgagg acgattcggg
      841 ggttcaccgc ccgcagccgg tcgtacccga ggccgaggtg gtcgagcgtc ccgggcgcga
      901 agctctccac gacgatttcg gcgcgctcga caaggccaag cacgaggtct cggccctccg
      961 gtcgcttgag gtcgacgacc accgaccgct tcgaggtgtt caaggccagg aacgcgccgg
     1021 agcgctcgat atgcggtacg tctttcggaa acggccccaa ctgccgtgcg gggtcgccgc
     1081 caggccgctc gatcttgatg acgtcggcac cgtagtcggc gaggacccga gtcgcgaatg
     1141 ggcccatcgc gtactgcgtc aggtcgacaa tccggacatc gtccattgga agcatggggt
     1201 cacctcgggc ggatgataac atccgagatg agcgatcaca ccactcgtcc agggcacccg
     1261 tgactttccg gcccgcagat cccagaatgc ggcagcaagc cgccgccgtc cgacgaggtg
     1321 ccccgcgatg ctcgtcttca tcctcttcgc cgccgtggcg ttcgccgtcg tggcgggcgg
     1381 agtcatgcgg cgccgccgtg tccgccggag ggtgcggcgg cgcatgttct ggcgtcggcg
     1441 cttctagccg agcgagattc gatcagaaag gggacgggcg atgtccgtgt acttcgtcat
     1501 ccaggaagaa atccacgacc cagaggcgct ggtcgagtac cgcgagaagg cccgcaactc
     1561 cccgcatgga ggcaagttgc tcgcaatgga cgacgcgccg atcgtcctcg agggcaactg
     1621 gcacggtggg cgcgcgctga ttatcgagtt cgacgatgag gcggcgttcc gggcctggta
     1681 cgaatcgccc gcctaccagg aggcgctgaa gatccgcctc gcggcgaccg attccaggtc
     1741 tgcgctactg cacggcatgc cctgaccgca agcgtgcgat cccgccgtcg cccgcgagcg
     1801 ggcggtcgga cgtgaagagg aggggagcgc gaccgctccc ctccttattc gtcgctttcg
     1861 cgggctatgg ccgaatggtc tcgaccaccg tcgttccgaa atggttcatc ccacgcgcgc
     1921 catgacgatc gaggatctca acgacacggt cctgcacctc ctgatcgcgt ccgcacttcg
     1981 actcccgcga aagatgcgga cgccctcgat gcccgacccg tcgaggtcgc gctccgcagc
     2041 caccgccgcg tcggcatcgt cgatgatggc caggaggtgg ccagtcgggt cggcgataaa
     2101 ctcctcgttg gcgggtgcgc cgcccggcgg tgagtcgtct ggcatcgtga tctcccccca
     2161 gttgcggttc agtcgacgcc caggagctcg aactcccatt cgcgtttcag gaactcgatc
     2221 tgctcgatga gcgcgcgctg ttgatcgaag ggcaggtcgc cgaacgggta gcgggcctcc
     2281 gccgaaccgc ggtagcgagt cgcctcgcgg ctgttctcgc caccgtcgat gcggcggatg
     2341 cgctcgcgga cctcttcgtc gctcacgggg acgtccggga tgccctcgag ggggcggtag
     2401 acaccgtccg aggcgtagaa gtagctcgcc gcctgctgca agaggtcggc atagcggtcc
     2461 gcgaacagct gatcctgcgg ggcgagcgca tggaaccagc gatcgaagat gtgcgggtcc
     2521 tgcgtcggga cggtgcgatg ctcctcgacc ttgccgcgcg gcgtccggat gcgatagcgc
     2581 acggccggcg gccgctcgac gaatccttca gggtcagccc actggttgtc caaggctaca
     2641 ttccttcgaa tccggcctcg cgcttctgct gagggcacca cctagccggc tcccgcgtct
     2701 tctgcgggtc gaagtagggc ctcgacctcg gcgattgcgt cggcaccctc gtcggtgtcg
     2761 ccgttctctc gggcatcgtt ccgtcctttc tgtagcgagc gtggcaactc gctccggtgc
     2821 acctcggtga gggtcgtcca gagcttgcgt gcgaactcag cgaggaggcg gccgctcggc
     2881 agcctgctgt cgcgcaggtc gtactgcatg aggtgcacgt cgagcagcca cagctcgtcg
     2941 gcggtcagcg cgagcgggat ctcctcgtcc gcgccatcga ggaagagggc catgagcacg
     3001 gcgcgaaagc ggcgggcgat ggcggcctgg ggatcggact cccccgacag ctgctcgcgc
     3061 gcttcccgcg agcgaggcaa gctgagcgtc tggacgagcc actgccgctc gtccggagcc
     3121 agccagagca ccgctcccag ttgcaccctg ttcagctcgt cttccatgtc atcacgctag
     3181 tccggccggt atctcactca cacaaataac cgcgggcacg ccaacacagc cggcgtgcga
     3241 tcagggccgg agcttgccga tcgcctcgct cagatcattc gggctgttga agcgatccat
     3301 cggcgggcgc tcgagggcgt gacgcacctc gtcatccgcc tgctgctcct gcgcgtgctc
     3361 caccaactgc tgcttgtcca cggggtagtc gatcccgccg aggaacttct gcacgttaat
     3421 cggattgact tctggcaagg gatgctcctt ccagcctcga taggggtcgc caccgtgcag
     3481 gcggcgcccg ccgatgctag ggagcgcgac gtttccgccg agttgcggag aggcaggcga
     3541 tgtgtcgggt cgctcgcagc cgagcgatcc gcttacggca ggaagtcgag cgggttgcgc
     3601 actgccccgt tcttgcggat ctcaaagtgg aggtgggcgc cggtcgagta gccggtgttt
     3661 ccactgatcg caatcgtgtc gccctggctc acctgctggc ccagtgagac gaggaacttt
     3721 gagaggtggc cgtagcgggt catatagcca tcggcatgct ggatatcgat gtagtagcca
     3781 tacgaacaac aggggtcccc accgacaaag acgaccttac cggaggcgct ggcggcaacc
     3841 ggctggaagg cagcggagat atcgatgccc agcggatgag cgggaccgaa gtaggacgtg
     3901 atgttgccaa cgagcggcca gatgaactga tagaccggct tcggcggcgc aaccggcgtg
     3961 ggttcgggcg tggaggcagc ctccgcgctc ggcgtggcct cgggggtggc ggcggcgggg
     4021 gcggcctgga cgaccggggg ggtgccgttc acgacgagga tctgggtgcc ctcgaggacg
     4081 ttgctcgggt cgtcgatgcg gttgccgggg aagccgacga tgtcgtcgac ggtgacgcca
     4141 tagtgctcag cgatgtccgc gagcgtctcg cccacgctga tgttgtggag cacgccgggg
     4201 acggtgggaa tctgcagccg tgcgcccacg agcaagagcg aacccgccga gatgacgccg
     4261 acgttgttat tgatgatgtc ctgcgtacgc accccggagc gggcggcgat gccactgacc
     4321 gtgtcaccgg ctcgcacggt gtaggtctgg tagagcgggg gcggggcggc cggagtagga
     4381 ctcgcagtcg gggtaggggt ggcctggttc tgaagcgcgg tctggcgctg atcggaggcg
     4441 gagccctcgg gggcgagggt ggcggtgggc gcaccgggag tcgccgcgga ctgactcggc
     4501 gcgggttgcg tggcggcgag ggccgcggtg acccaggagg ggagctcggc cgcggcggga
     4561 gacggggtgg cgctcgggac ggtagcggcc ggacggctcg gcgggttcga ggcgagctcg
     4621 gcggcgggag ttacatcggc caaggtcata ttggagacgg cgggcaggcc gggcaacgtg
     4681 gcagcggcgg ccagcgtgcc atcgtgggcg tcagcggagg tgagcccagc gaaccaggcc
     4741 gccccgacga cggcgctcac tccaagcaca aggatggcga ggaacgcatg cgtgtggacg
     4801 cgcggccctt gccgctcaga gtcggcggcg aacttcctcg cgcgcaggag actgcggtat
     4861 tcccgtcctc gaggcgcggg aaggggcctc gggacgcggt cgccgcgatc gggtccagga
     4921 ataggttgcc cccaaccaga ccccgccgcc ggaaatagta acgtgtcctc acgatagggc
     4981 gcacctcgaa gttcggctgg tgaccacgat tgtgaaatca gtcccgaaat gtttcacgtg
     5041 aaacatctgt tcttggcgcg caactgcgcc tcgtgtgcgg gtaccatggg ccgcggcggg
     5101 gtactgggcc ccttacgggc ggacgcgcat acggcgcggc tcggaggtac ctcgtggcac
     5161 tcgcggaaca ccttcccctg ctcggtggac cgaccctgcg cgagcgacgg ctcacgcgtc
     5221 gggaggtgct ggccggcctg ggcggggcgg gggcggcggc agcgctgatc gggccagg
//
LOCUS       DAORPG010000052         5267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_961_length_5267_cov_3.478127, whole genome shotgun sequence.
ACCESSION   DAORPG010000052 DAORPG010000000
VERSION     DAORPG010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_961_length_5267_cov_3.478127"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PLX85_02005"
     CDS             complement(<1..487)
                     /locus_tag="PLX85_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRC61981.1"
                     /translation="MMAEALLGVWTTQIPFARREITGEVRRSPSLPPGRTLLAVDLSA
                     DGSAPAYATGSAAAGAEDAIVGRLVEAARRGDAEAFGGLYDRFQPEILRYLTYQLRDR
                     DTAEDLTQQAFLNAWRAIPRYEQRSVPVRAWLYRIARNQLLDHLKRRRRVTVDVDLVN
                     PA"
     gene            617..2044
                     /locus_tag="PLX85_02010"
     CDS             617..2044
                     /locus_tag="PLX85_02010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC61982.1"
                     /translation="MSLPPQFDEPAFRGLRRIDAALDRGQTRVLRVLWFMLPAGSVAR
                     RPQFQALLASRFLSDVAIQALLYGSLIATVRQGGTEVHAALLGIAYLLPGVVLALYGG
                     ALADALPKRLALVAAYLAMGLLCFVITGTFGTGFRSLLLVIFSVRALHQLSQPSEASA
                     VPLVATREELASATSFLSFASSAGEVAGKALLAPVVVRVWGVDPVIVLAGCLFVLSSS
                     RALKLETRSLAGGPKRNPSQIMAVRRQFSSRVALQYLVAQPGVLWMLLLAAMASTVNV
                     VLGVLGPQYVQSVLDVDPSNAVYVFAPAPIGLLLALALAPILIESLGERVVAGVGFGL
                     VAVAGTSLGLVQPLSSHAGWLLVLDIPGVNSRVEFAAMLSVLLGAGMTLAAAAAQTFV
                     TRNVPLEIQGRTNAPLGMLKDGLAIPSLLLLGSVAGVVGVRSVITVAPGILLVAALFV
                     DRYSARWRAATGENRILGRGPAASR"
     gene            2131..3138
                     /locus_tag="PLX85_02015"
     CDS             2131..3138
                     /locus_tag="PLX85_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRC61983.1"
                     /translation="MRFASARVLVPLALILVTLIAVVPHISASERGPSTYIVQPGDTL
                     SEIADRFGTTVEAVVALNGLDDAGRIVAGQSLRLTSGGSDGAPSSRVYRVQPGDTLWE
                     IAQAQGVSVPDLARANGMSITDVLRTGMELHIPSTAPIGTAPADRIHVVSAGETLSSI
                     ADDFAVTVDSLRSANGLESDLIRVGQTLRVPQQALPQLSKQTAAALHEAASESGVDPY
                     LLLALSLMESGWQNHVVSSTGAVGLMQLMPETAEWTVDHLATGADHWDGSLEDNARVG
                     AAYFGHLLFIEGGDVEGALASYYQGWASYKQYGMYEETRDYVDDVLALEARLRARGTS
                     G"
     gene            3261..3602
                     /locus_tag="PLX85_02020"
     CDS             3261..3602
                     /locus_tag="PLX85_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61984.1"
                     /translation="MDGEAQLPPFVFHGTPNAYTNYGCRCDACREAHSLYRSERRARD
                     PAKYTTYMREYMRKYRARKRQEREALCANCGEQPSAVIAEDGKRYCLECAERLEGIGA
                     VRPIPKPAATE"
     gene            3692..3919
                     /locus_tag="PLX85_02025"
     CDS             3692..3919
                     /locus_tag="PLX85_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61985.1"
                     /translation="MFGEGGVRVVGGRVVLLSAVALGSFVYALLLVAQPGALDSEADS
                     DAPDWRVLAKGGGAFGLAVVLMFVAILMIDV"
     gene            complement(3992..>5267)
                     /gene="groEL"
                     /locus_tag="PLX85_02030"
     CDS             complement(3992..>5267)
                     /gene="groEL"
                     /locus_tag="PLX85_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRC61986.1"
                     /translation="PMALKRGIETAARTISEQISRNSTKVTTREQMAQVAAISAASPE
                     IGSLIGDVMEATGKDGVITVEESRGINTEVEYVEGMQFDRGYVSPYFVTNPERMEAVI
                     DEPYIFVTDQKLSSVNDILPLLEKQLQVSKDIVIIGEDIEGEALATLAVNRLRGTISC
                     VAVKAPGFGDRRKENISDIAALTGATVISPELGRSLDSAEPADLGRARRVVVTKEETT
                     IIEGRGDKKRIDEQVSMIKAQLDAATSDWEREKLQERLGRLSGSVAVIKVGAATEVEL
                     TEKKHRVEDALSATRAAVEEGIVPGGGVAFINALGALDKVGLEGDEGTGVRILRRALE
                     EPLRRIAANAGEDGSVIVRQVQSLKRGEGYDAAKGRYGSMMEFGIIDPAKVTKAALDN
                     AVSIAGMVLTTNCLVTDLKEEQSAGAAAAGMY"
BASE COUNT          771 a         1697 c         1822 g          977 t
ORIGIN      
        1 cggccggatt cacgaggtca acatcgacgg taacgcgccg ccggcgcttg aggtggtcga
       61 gcagctgatt gcgggcgatg cgatagagcc acgcccgaac gggtacgctt cgctgttcgt
      121 agcgggggat ggcgcgccag gcgttcagga atgcctgctg cgtcagatcc tccgcggtat
      181 cgcggtctcg gagctggtag gtgaggtaac ggaggatctc cggctggaag cggtcgtaga
      241 gcccgccgaa cgcctcggca tcgcctcggc gtgcggcctc gaccaggcgc ccgacgattg
      301 cgtcctcggc gcctgcagcg gcactccctg ttgcgtacgc cggggccgac ccgtctgcac
      361 tcagatccac ggcaagcagc gtacgaccgg gcgggagcga gggggagcgg cggacttcac
      421 cggtaatctc tcgtcgtgca aacgggatct gggtggtcca tacgccgagc agtgcctccg
      481 ccatcatcgc ctcgatcgtg gattgcgggc caaccggcgg cgtcccagcg cgcctcactc
      541 catgtaacga ccgcgcggac gattcgttcg tccgggctcg aatgcctggc gggctgcttc
      601 cgctacgatg ctgtccatgt cgttgccgcc gcagttcgac gaaccggcgt ttcggggcct
      661 gcgccggatc gacgccgccc tggaccgggg acagacccgt gtacttcgag tgctgtggtt
      721 catgctgccg gccggctcgg tcgcccggcg gccgcaattt caggccctgc ttgcttcccg
      781 gttcctgagc gacgtcgcca tccaggccct gctctacggc tcgctgatcg ctacggtgcg
      841 ccagggcggc accgaggtgc acgcggcgtt gctgggcatc gcgtacttac tgcccggcgt
      901 ggtgcttgcg ctgtacggag gggcactcgc cgacgctctc cccaagcgac tggcgctcgt
      961 cgcggcctac ctcgcgatgg ggctgctctg ctttgtgatc acgggcacgt tcggcaccgg
     1021 gtttcgctca ctcctgctgg tgatcttttc agtgcgcgcg ctgcaccagc tatcgcagcc
     1081 gtcggaggcc tcggcggtcc cgctggtcgc cacgcgcgag gagcttgcct cggcgacgtc
     1141 atttctctcg ttcgcatcat cagcgggcga ggtcgccggg aaggcactcc tcgcgccggt
     1201 ggtcgtccgc gtctggggtg tcgacccggt catcgtgctc gcgggttgcc tgttcgtcct
     1261 gtcctcgtca cgagccctaa agctcgagac gaggtcgctc gcgggcgggc cgaagcggaa
     1321 cccctcgcag atcatggcgg tgcgccggca gttctcgagc cgggtggccc tgcagtacct
     1381 cgtggcgcag cccggcgtgt tgtggatgtt gctgctcgca gcgatggcaa gcacggtcaa
     1441 cgtggtactg ggtgtcctcg gcccgcagta cgtgcaatcc gtcctcgacg tcgacccgtc
     1501 aaacgcggtc tacgtcttcg ctccagcgcc catcgggctg ctgctggcgc ttgccctcgc
     1561 gccgatcctg attgagtcgc tgggcgaacg tgtggtcgca ggagttggct ttggtctggt
     1621 cgccgttgcg gggacgtccc tgggtctggt ccagccactt tcgagccacg ccggatggct
     1681 cctcgtcctc gacatccccg gggtcaacag ccgggtcgag ttcgcggcga tgctctcggt
     1741 actcctcggc gcgggtatga cgctggcagc cgccgcggcg cagacgttcg tgacccgaaa
     1801 tgtaccgctc gaaatccagg ggagaacgaa tgcgcccctc ggcatgttga aggacgggct
     1861 ggcgatcccg agcctgctgc tactcggttc ggttgccggg gttgtggggg tgaggtcggt
     1921 gatcaccgtc gcgcccggca tcctgctcgt cgccgcgctc ttcgtggacc ggtattccgc
     1981 gcgctggcgc gcggcgacgg gggagaatcg catcctcggc cgcgggcctg cggcatcccg
     2041 gtaggggttg ccgaggcccg cggggtccgt gctacgctac ctgcggcggg tattggatgg
     2101 accttgcgca ctccccactc tgcccgctcg atgcggttcg catcggcgcg tgtcctcgtc
     2161 ccgcttgcac tcatcctcgt tacgctgatt gcggttgtgc cgcacatctc ggcgtctgag
     2221 cgcggccctt cgacgtacat cgttcagcca ggagacacac tgtccgagat cgcggaccgt
     2281 ttcggcacga ccgtcgaggc cgtggttgcg ctgaacggcc tcgatgacgc gggccggatc
     2341 gtcgccggtc agtcgttgcg gctgacctcc ggaggctcgg acggcgcgcc atcgagccgc
     2401 gtatatcgcg tgcaaccggg cgatacgttg tgggagatcg ctcaggcaca gggtgtatcg
     2461 gtcccggacc tcgccagagc gaacggcatg agcatcaccg acgtcctgcg gaccggcatg
     2521 gagctgcaca tcccatcgac ggcgccaata ggcactgcgc cggcggatcg aatccacgtg
     2581 gtgtcggcgg gtgagactct gtcgagcatc gcagacgact tcgcggttac tgtcgacagc
     2641 ttgcgctccg cgaacggcct tgagagcgac ctgatccggg tcggccagac cctgcgcgtc
     2701 cctcagcagg cgctgccgca gctctcgaag cagacggccg ccgcgctgca cgaggccgca
     2761 tccgagtcgg gcgtcgaccc gtacctcctg ctcgcgcttt cgctgatgga gagcgggtgg
     2821 caaaaccacg tcgtcagcag caccggagcg gtcgggctga tgcagctcat gccagagacg
     2881 gcggagtgga cggtcgacca cctcgccact ggtgcggatc actgggacgg gagcctcgag
     2941 gacaacgcgc gcgtgggtgc ggcctacttc ggccacctgt tgttcatcga gggcggcgac
     3001 gtcgaagggg cgctggcctc gtactaccag ggctgggcca gctacaagca gtacgggatg
     3061 tacgaggaga cgcgcgacta tgtggacgac gtactcgcgc tcgaggctcg tctgcgtgcc
     3121 cgggggacga gcggatgacc gcggcacgca gtcgcgggta ggcgacaaga cgtaacacct
     3181 gtacgatgtg aagacgctct ttcgtttccc agtgcctcaa caagcgcgcc tcatgtacgt
     3241 ctctttttgg gaggctagcc atggacggcg aggcacagtt gccgcccttc gtgttccacg
     3301 gaactccgaa cgcatatacc aactacggct gccgatgcga cgcatgccgc gaggctcata
     3361 gcctgtatcg cagcgagcgt cgcgcacgcg atccagccaa atacacgacc tatatgcggg
     3421 agtacatgcg gaagtaccgg gcacgcaagc gccaggagcg cgaggcattg tgcgccaact
     3481 gcggtgaaca gccgtcggcg gtcatcgccg aagatggcaa gcgctattgc ctcgagtgcg
     3541 cagagcggct cgagggcatc ggcgcggtgc ggccgattcc caagccggcg gccaccgagt
     3601 aagcacgaac ccaggggaat gcgaacaggg ggaggtgaga cctccccctg ttttcgtgac
     3661 gcccgcggac gttcactcga ggcgcatact gttgttcggc gaaggggggg tgcgggtggt
     3721 cggtggacga gtcgtgctgc tcagcgccgt cgcgctcgga tcattcgtgt atgcgctgct
     3781 attggtcgct cagcccggtg cgctggatag cgaagccgat tcggacgctc cggactggcg
     3841 agtgctcgcc aaaggaggcg gcgcattcgg cctggccgtc gtgctgatgt tcgtcgcgat
     3901 cctgatgatc gacgtatagg tccagcacat cccccaaacg tgaagaaggc cggcatacgc
     3961 cggccttctt catcatcctg tgtacccgcc tctagtacat gccggcggcc gcggcaccag
     4021 cgctctgctc ctccttgagg tccgtgacga ggcagttcgt cgtgaggacc atgccggcga
     4081 tggagaccgc gttgtcgagc gccgccttcg tgaccttggc ggggtcgatg atgccgaact
     4141 ccatcatgct gccgtagcgg cccttcgcgg cgtcgtagcc ttcaccgcgc ttcaggctct
     4201 gcacctggcg gacgatgacc gagccatcct cgccggcgtt cgcggcgatg cggcgaagtg
     4261 gctcttcgag ggcccggcgg aggatgcgga cacccgtacc ctcgtcaccc tcgaggccca
     4321 ccttgtcgag cgcgccgagc gcgttgatga atgcgacgcc accaccggga acgatgccct
     4381 cctcaacggc ggctcgggtg gcggagaggg cgtcctcaac gcggtgcttc ttctcggtca
     4441 gttcgacctc ggtggcggcg ccgaccttga tgacggcgac cgagccggag aggcggccga
     4501 ggcgctcctg gagcttctcg cgctcccagt cgctggtggc ggcgtcgagc tgggccttga
     4561 tcatcgagac ctgctcgtcg atgcgcttct tgtcgccgcg gccctcgatg atcgtcgtct
     4621 cttccttggt gacgacgacg cggcgggcgc ggccgaggtc ggccggctcg gcggagtcga
     4681 gcgagcgtcc cagctcaggg ctgatgaccg tggcgccggt gagggcggca atgtcggaga
     4741 tgttctcctt gcggcggtca ccgaagccgg gcgccttcac ggcgacgcag ctgatggtgc
     4801 cgcggaggcg gttgaccgcg agcgtcgcga gcgcttcgcc ttcgatgtcc tcgccgatga
     4861 tgacgatgtc cttggaaacc tggagctgct tttcgagcag cggaaggatg tcgttcacgg
     4921 aggagagctt ctggtccgtc acgaagatgt agggctcgtc gatgacggct tccatccgct
     4981 ccgggttggt gacgaagtac ggcgagacgt agccgcggtc gaactgcatg ccctcgacat
     5041 actcgacttc ggtgttgatg ccgcgcgact cctcgacggt gatgacgccg tccttgccgg
     5101 tcgcctccat gacatcacca atcagcgacc cgatttcggg cgaggcggca gagatggcgg
     5161 ctacctgcgc catctgttcg cgcgtggtga ccttggtgga gttgcgggag atctgctcgc
     5221 tgatggtgcg tgcggcggtc tcgatgccgc gcttgagggc catgggg
//
LOCUS       DAORPG010000053         5232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_980_length_5232_cov_3.454703, whole genome shotgun sequence.
ACCESSION   DAORPG010000053 DAORPG010000000
VERSION     DAORPG010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_980_length_5232_cov_3.454703"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PLX85_02035"
     CDS             complement(<1..469)
                     /locus_tag="PLX85_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61987.1"
                     /translation="MALPRRRVLMVPVTGIAVAVAVAILLVNRDERPRDEGTPAPAST
                     SLLFAEFGTNADRVFRAEARDPSVRSLVATIPHAPEWGINAAARPAGDLVAFTVLPAT
                     ARPERDTPAELRVLDLADGRGCHVVGLAIHRGFDVAGVGDLGNWLRRQRPGLFD"
     gene            646..1389
                     /locus_tag="PLX85_02040"
     CDS             646..1389
                     /locus_tag="PLX85_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRC61988.1"
                     /translation="MLLRYLSVLGEAPSAFLILIGAFLISMLLGLAFHEFCHGLIANS
                     LGDPTPKRYGRLTLDPRAHLDPVGSLLILFVGFGWARPVPVNPYNTKNPQASMVAIAA
                     AGPLSNLLVAGLAGIPIKLGMVPFFHPFVNPGDAAFWARIWAQSPDNLIGLFLGTIVL
                     LNVILAVFNFVPIAPLDGFRVLVGLLPRDLSREVGKYEAWGPGVLLILFMLPFLTNGQ
                     ISPLFDIMAPPIEFFLHLFAGDSGGLRVA"
     gene            1379..1897
                     /locus_tag="PLX85_02045"
     CDS             1379..1897
                     /locus_tag="PLX85_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRC61989.1"
                     /translation="MWHSAAYRSGQFFRGFRWALRPDEVSRVCGLLNERERLLFSAMQ
                     GRDRRHSMDMVRWLERRGAAKGTPPSADLLAAALLHDVGKGSLIVWDRVAFVLLGACS
                     IRLRRSLERERGWRWQQALWRLEHHAGLGAELLEGSGTRPRVVELVRRHKDPGADGDT
                     ELSALMAADGAC"
     gene            1956..2984
                     /locus_tag="PLX85_02050"
     CDS             1956..2984
                     /locus_tag="PLX85_02050"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRC61990.1"
                     /translation="MPRVAILGTGLIGGSIGLRLKAARPELEVVGFDSFREHSQAAER
                     GKAIDRVAHSPREAVTGAGLVIFAAPILAIRDLMQEVAPALEPGVIATDTGSTKAEVM
                     RWARELLPAEASFVGGHPMAGKTEAGPSAAEATLFEGARWAIVPSSEAAEDAVDVVTG
                     LALAMGARPMFLDAEEHDAYVAAISHLPLMVATALFRLTRDSEAWPELSMLAAGGFRD
                     TSRLAGTDPAMAHDIAVTNRTQLIHWIERYIETLRSLQRQVADVEGEQELFRVFAQTN
                     LEHGAFMSGAIGRKEIDEAHWSAVPETSLMDLLLGGTMAERAREMTRRSEERLAEAER
                     RAKAGGRG"
     gene            2984..4315
                     /gene="aroA"
                     /locus_tag="PLX85_02055"
     CDS             2984..4315
                     /gene="aroA"
                     /locus_tag="PLX85_02055"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRC61991.1"
                     /translation="MSDEMRVRRPARLRGTIAIPGDKSVTHRAIMFNSIAAGDARIDG
                     FLDAADTRSTLECMRALGAEIEDLGGGVLVVHGRGRGGLHEADNVLDCGNSGTTMRLL
                     TGLVSGLPMLSVLTGDDSLRRRPMARVVRPLAQLGAEITARAAGTLPPVVVQGGPLRG
                     GQRVDTAVASAQVKSALLLAGLAADGPTTVSEPSASRDHTERMLAAMGAGIATVEDGG
                     RHVVTLTPPATDLVAVDVTVPGDISTAAVWMVLASLHPDAELTLMNVGINPTRTGLLD
                     ILWGMGADITLIEERVTGGEPVADLRVRSARLKGIEVGGELVPRAIDELPLVALAGAL
                     AEGETLIRDAEELRVKESDRVASTAAVLRAFGAEVEERPDGMLVRGGATLHAGSAESR
                     GDHRLAMLGAAAGVLAGGVSTIVGGGAVVVSYPEFWEELLRLQAAEGAVSR"
     gene            4326..4586
                     /locus_tag="PLX85_02060"
     CDS             4326..4586
                     /locus_tag="PLX85_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HRC61992.1"
                     /translation="MTTELIHVGFENHLALNRVVAVATPASAPVKRMIQRGKEERHII
                     DLTSGRRTKAVVVLDNDWVALLAITPETFAGRVGALRNVDGS"
     gene            4589..5218
                     /gene="gmk"
                     /locus_tag="PLX85_02065"
     CDS             4589..5218
                     /gene="gmk"
                     /locus_tag="PLX85_02065"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRC61993.1"
                     /translation="MAEVPPHLVVVISGPSGVGKDTLVDRLLAEHPEFSRPVTMTTRA
                     PRPGEVDGRDYVFVTPEEFHRRIDAGELLEHAFIYGNLYGLPRTQLEQALERGDAVVR
                     VDVQGVASLRKLLPAAVYIMLVPDSFEHLERRLRGRGEAHDDADLERRVAASREELTR
                     TDLFDHVVENVEGDLDATIAAVIRIVADERRRVQASRHDSGCTPVGAAR"
BASE COUNT          756 a         1794 c         1870 g          812 t
ORIGIN      
        1 tgtcgaacag gcccgggcgc tggcgtcgga gccagttccc caggtctccg acgccagcga
       61 cgtcgaagcc ccggtggatc gccagcccga cgacatggca gcccctgcca tcggcgaggt
      121 cgaggacgcg cagctcggcg ggggtatcgc gctcggggcg tgccgtcgcc ggcagcaccg
      181 tgaaggcgac caggtcgccc gccgggcggg cggcggcgtt gatgccccat tccggcgcat
      241 gcgggatggt cgcgacgagc gaccggaccg aggggtcacg cgcctccgcg cggaacaccc
      301 gatccgcgtt cgtgccgaac tccgcgaaca gcagcgaggt gctggcgggg gcgggggtgc
      361 cctcgtcgcg gggacgttca tctcgattca cgaggaggat ggcaacggcg accgcgacgg
      421 cgatgcccgt caccggaacc atgaggacgc gacggcgggg cagggccatg tacgctcacc
      481 atgcaaacga ggggttccga aaccccctgc gtagcgagcc ccgccagctc gataccgcct
      541 gctgagtggc ccggggcgcg cgctggggtt gagcgcaccg agtgggatca ccataggaac
      601 gcccgtcgag gtgtgtcaaa ccaacggcgg acgtatcctg atcgggtgct gctgcgctac
      661 ctgtctgtgc tcggggaagc gccgtcggcg tttctcattc tcatcggcgc attcctcatc
      721 tcgatgctgt tggggctcgc gtttcacgag ttctgccacg gcctgatcgc gaacagcctc
      781 ggcgatccga ccccgaagcg gtatggccgc ctcactctcg acccacgcgc gcacctcgat
      841 ccggtggggt cgttgctgat cctctttgtc gggttcggct gggcgcgtcc cgtgccggtg
      901 aacccgtaca acacaaagaa cccgcaggcc agcatggtcg cgatcgcggc ggcgggaccg
      961 ctgtcgaacc tgctcgtcgc gggactcgcg ggaatcccaa tcaagctcgg gatggtgccg
     1021 ttcttccacc cgttcgtgaa cccgggtgac gcggcgttct gggcgcgtat ctgggcgcag
     1081 tccccggaca acctgatcgg gctcttcctc ggcacgatcg tgctgttgaa cgtgatcctc
     1141 gccgtcttca acttcgtgcc tatcgcgccc ctcgacgggt tccgggtgct ggtcggcctg
     1201 ctgccgcggg acctgagccg ggaggtcggg aagtacgagg cgtggggacc gggcgtgctg
     1261 ctgatcctgt tcatgctccc attcctcacg aacgggcaga tcagccccct gttcgacatc
     1321 atggcgccgc cgatcgagtt cttcctgcac ctcttcgccg gcgacagcgg cggactccgt
     1381 gtggcatagc gcggcatacc gctcgggtca gttcttccga ggcttccgct gggcgctgcg
     1441 gcccgacgag gtgtcccgcg tctgcggact cctcaatgag cgtgagcgcc tgttgttttc
     1501 cgccatgcag ggccgcgacc gccgtcactc gatggacatg gtgcgctggc tcgagcggcg
     1561 cggtgcggcg aagggcactc caccctcggc cgacctgctg gcggcggcgc tgctgcatga
     1621 cgtcggaaag ggctcactca tcgtgtggga ccgcgtcgcg ttcgtcctcc tcggggcgtg
     1681 ctcgatccgt ctccgccgct cactcgagcg tgagcgaggc tggcgctggc agcaggcgct
     1741 atggcggctg gagcaccatg cgggccttgg tgcggagctg ctcgagggga gcgggacgcg
     1801 tcctcgcgtc gtcgagctgg tgcgacgcca caaagacccc ggggcggacg gcgacaccga
     1861 gctgagtgcc ctgatggccg ccgacggcgc gtgctagcgc cgccgagcac ctcggcctcg
     1921 gtacactgat cgaatacatg ggagaggggt gagtcatgcc tcgggtcgcg atcctcggca
     1981 ctggcctcat cggcggctcg atcggtctgc gtctgaaagc agcgcgcccc gagctcgagg
     2041 tcgtcggctt cgactcgttc cgcgagcact cgcaggcggc tgagcgcggc aaggccatcg
     2101 accgcgtcgc gcattcgccg cgcgaggccg tgacgggcgc gggcctggtg atcttcgccg
     2161 caccgatcct cgcgatccgc gacctgatgc aggaggtcgc gcccgcgctg gagccggggg
     2221 tgatcgcgac cgacaccgga tcgacgaagg ctgaggtcat gcgctgggcg cgcgaactgc
     2281 tgccggccga ggcctcgttc gtgggcggcc atcccatggc ggggaagacc gaagccgggc
     2341 cctccgccgc ggaggcgacc ctcttcgagg gcgctcgctg ggccatcgtg ccttccagcg
     2401 aggccgccga ggacgcggtc gacgtagtca cggggctcgc gctcgccatg ggcgcccgcc
     2461 cgatgttcct cgatgccgag gagcatgacg cctacgtcgc cgcgatctct cacctgccgc
     2521 tgatggtcgc gacggcgctg ttccggctca cgcgggactc cgaggcctgg ccggagctgt
     2581 cgatgctcgc cgccggaggc ttccgggaca cgtcacgcct cgcgggcacc gacccggcga
     2641 tggcccacga tattgccgtc accaaccgca cccagctaat ccactggatc gaacgctaca
     2701 tcgagacgct gcgcagtctg caacggcagg tcgcggacgt cgagggcgag caggagctgt
     2761 tccgtgtctt cgcgcagacg aacctggagc acggcgcgtt catgagcggt gcgatcggcc
     2821 gcaaggagat cgatgaggcg cactggtcgg ccgtgccgga gacgagcctg atggacctgc
     2881 tcctcggcgg gacgatggcg gagcgcgccc gcgagatgac gcgccgctcc gaggagcgcc
     2941 tcgcggaagc ggagcggcgc gcgaaggcgg gaggccgcgg ctgatgtccg atgagatgcg
     3001 agtgcgccgc cctgcccgcc tgcgcggcac catcgccatt cccggcgata agtccgtcac
     3061 ccaccgggcc atcatgttca actcgatcgc ggccggggac gcccgcatcg acggcttcct
     3121 cgatgcggcg gacacgcggt cgacgctcga atgcatgcgc gcgctgggag cggagatcga
     3181 ggacctcggg ggcggggtgc tggtcgtgca cggccgcggc cgcggcgggc tgcacgaggc
     3241 ggacaacgtc ctcgactgcg ggaactccgg cacgacgatg cgcctgctca cagggctcgt
     3301 ctcgggcctg ccgatgctct cggtgctcac tggcgacgac tcgctgcgcc gccgtccgat
     3361 ggcgcgcgtc gtccgtccgc tggcgcaact gggcgccgag atcaccgcac gggcggcggg
     3421 taccctgccg cccgtggtcg tccagggagg gccgctccga ggtggccagc gcgtcgatac
     3481 cgcggtcgca tcggcgcagg tgaagtccgc gctgctgctc gcgggcctgg cggcagacgg
     3541 ccccacgacc gtgtctgagc cctcggcatc acgtgaccac acggagcgca tgctcgcggc
     3601 catgggcgcg ggcatcgcga cggtcgagga tggcggccgg cacgtagtca ccctgacccc
     3661 gccggcgact gatctggtgg cggtggacgt tacggtgccc ggcgacatca gcacggcggc
     3721 cgtgtggatg gtgctcgcct cgctgcatcc cgacgcggag ctgacgctca tgaacgtggg
     3781 gatcaacccc acgcgcaccg gactcctcga cattctgtgg gggatggggg ccgacatcac
     3841 cctgatcgag gaacgcgtca ccggcgggga gccggtcgcc gacctccgag tgcgttcggc
     3901 gcggctgaag ggcatcgagg tgggcggcga actcgtgccg cgcgccatcg acgagcttcc
     3961 gctcgtcgcg ctggccgggg ccctggccga gggcgagacg ctgatccgcg acgccgagga
     4021 gctgcgagtc aaggagtcgg accgcgttgc ctcgacagcc gcggtgctgc gcgccttcgg
     4081 cgcagaggtc gaggagcgtc ccgacggcat gcttgtccgg ggcggcgcga ccctccacgc
     4141 gggcagcgca gagtcgcgcg gcgaccatcg cctggcgatg ctgggagcgg ccgcgggcgt
     4201 cctcgcaggc ggcgtctcca cgatcgttgg tggaggggct gttgtggtgt cgtatcctga
     4261 attctgggag gaattactac gtcttcaggc agcggagggt gccgtatctc gttgaggaac
     4321 agccaatgac caccgaactg atccacgtcg gattcgagaa ccatctcgca ctgaaccgcg
     4381 tcgtcgcggt tgccacgccc gcatccgccc ccgtgaagcg catgatccag cgcggcaagg
     4441 aggagcggca catcatcgac ctgacgagcg gccgccgcac caaggccgtt gtcgtgctcg
     4501 acaacgactg ggtcgcgctt ctggcgatca cgccggagac cttcgccggc cgcgtgggcg
     4561 ccctgcgcaa cgtcgacggt agctgagctt ggccgaggtc ccaccgcacc tcgtggtcgt
     4621 catctccggg ccctccgggg tcgggaagga cacgctcgtc gaccgcctgc tcgcggaaca
     4681 ccctgagttc tcccgccccg tcaccatgac gacccgggcg ccccgtccgg gcgaggtgga
     4741 cgggcgcgac tacgtcttcg tgactcccga ggagttccac cgccgtatcg atgcgggcga
     4801 actccttgag cacgcgttca tctacggaaa cctgtacggg ttgccgcgca cccagctgga
     4861 acaggccctc gagcgtggcg acgcggtcgt tcgggtggac gtgcagggcg tcgcctcgct
     4921 gcggaagctg ctgcccgcgg cggtctacat catgctcgtg cccgacagct tcgaacacct
     4981 cgaacgccgt ctgcgcgggc gcggcgaggc acacgacgat gccgacctcg aacgccgcgt
     5041 cgccgcttca cgcgaggagc tcacgcggac cgacctcttc gaccacgtcg tcgagaacgt
     5101 cgagggagac ctcgatgcca ccatcgccgc cgtcatccga atcgtcgccg acgaacggcg
     5161 gcgcgtgcag gcgtcccgtc acgattcggg ctgcacgccg gtcggcgcgg cgcggtagcc
     5221 ggcggtcgag ag
//
LOCUS       DAORPG010000054         5217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_986_length_5217_cov_3.843665, whole genome shotgun sequence.
ACCESSION   DAORPG010000054 DAORPG010000000
VERSION     DAORPG010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_986_length_5217_cov_3.843665"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1902)
                     /locus_tag="PLX85_02070"
     CDS             complement(<1..1902)
                     /locus_tag="PLX85_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61994.1"
                     /translation="MSIVEELMARHDPALRGHGPAVPEEFAVALRSMEPILSPEDLEA
                     WAAAGVDLAGRSLRSWEAAVEYFRVGPRIANQLPFNLVMYCAETAGRITEGSPPVAAA
                     FLRAAPEVIPLLPLERGDSVGIMGEEIPLRHAGQGEIGRWARMGERLHRGNWKSVALA
                     SLFYELTPRLLDPLRLHLVEELVEVIDLLSDRSYQLATICLESSPELFAGLRPADREP
                     FLRFARAVTRASWADTRLYFERGPQLIQGVEQDDRETFLDIAAKVVTAGGRQGFPHFV
                     DAATALATVPTAEHQVLIALATHIAKGSPVAAMSLLSSAPSVLTRLTTDQLGQWAEPG
                     SRLLFEEGNIEGAEAYFRLESVRAEQALAALSARVELTTVGSLLRMYAKALTGEQVAI
                     RSAEELVDKGIGWVTESVATTEGTSIYLPPFVGSFPDQAANFQCYKVFTTHQTGRIEF
                     GSFAFRFGEPGVHTPATVRDREAQLPARERPVITSMERFYDLFEDRDLIAALFTIVED
                     TRIDAYVAREYGGIRVWLQRLQAFEAERRPDVRTMALRAAFCENLLRSSLGRPDRMLW
                     PRELSDLLRQGLNALKIVEQPGALVQDSAEVAAALYDLVTLIPNLLNAFAESDWVEPD
                     EDMLTMYPSM"
     gene            complement(1899..2501)
                     /locus_tag="PLX85_02075"
     CDS             complement(1899..2501)
                     /locus_tag="PLX85_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRC61995.1"
                     /translation="MRLGIVSDIHCNIASLDMALSRMGHVDEVLCAGDAIFQFRFSNE
                     VIGRLKEIGAHVVLGNHEEVVLSRGGQRVREQPDVDQDLLKWLSERPKRIDTVIDGKR
                     ITMFHGSHRDCGEYVYPHNAVMKEFGNLGADFVIHGHTHCAMTHQVNGTLVINPGSAG
                     QPRDPWNGFRSSYAILDTQSGEVTFDVYDDPVHKPNSVTS"
     gene            complement(2527..3384)
                     /locus_tag="PLX85_02080"
     CDS             complement(2527..3384)
                     /locus_tag="PLX85_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HRC61996.1"
                     /translation="MAEQAFKQMQIEDFRLTSEPYYLPIADEVQLFETAYDQNVPVLL
                     KGPTGCGKTRFVEYMSWRLHGHLGSRDDEGVPLITVACHEDLTASDLVGRYLLEGEET
                     RWIDGPITRAVKVGAICYLDEIVEARKDTTVLIHPLTDYRRLLPIDKRGDLLEAAPGF
                     LLVLSYNPGYQSALKDLKHSTRQRFIAIEFDYPPRDQEAEIIRTESGCSQEMANELAK
                     LAEKVRNLKEHGLAEGVSTRLLVYAGKLIASGIAPRRACQSAVVWGLTDDLEVQRSLE
                     EVVTSIFAD"
     gene            complement(3399..4070)
                     /gene="coaE"
                     /locus_tag="PLX85_02085"
     CDS             complement(3399..4070)
                     /gene="coaE"
                     /locus_tag="PLX85_02085"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRC61997.1"
                     /translation="MTVIGLTGGIASGKSTAASLLKGYGAIVIDADRVGHRVYEPGTS
                     GFQAVVNEFGHDLVAADGTINRQLLGGKVFGDPAQMKRLTDIVWPEIRKLAAEEIADL
                     KKRDPASTIVLEAAVMIEANWMDLADEVWVVTVSPEVARERLMARNGLTEAQAQARID
                     SQLSNKERLAYADVKVDNSSTLEQFEQRVEREWKKLLKRVDEANAANAKAGAADKGTK
                     RAARK"
     gene            complement(4266..>5217)
                     /locus_tag="PLX85_02090"
     CDS             complement(4266..>5217)
                     /locus_tag="PLX85_02090"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRC61998.1"
                     /translation="GELADGKLSPVTAELLGAAPRVADGGKVSVTLLGSAAAGAAADA
                     FAAGADRACVSSDSGYDDFRSDQWLSAVEAAVDQTSAGVVLIAQSGVGRDLGPRLAFR
                     RNTAVAMDCLRVENVDGSLRATRSAYGGNAHATYSFATSPAVVTVRAKSQDPIEPRAG
                     ASGETVELPAAGASRTEITGREAAVSEGLQIQDAPIVVSGGRGLGAPEGFQVIEELAA
                     AFGSDKAAVGASRAACDLGWYPPSQQVGLTGKIVSPDLYVAVAISGASQHMAGCSGSK
                     TIVAINRDPEANIFKSARFGIVGDYKAILPELISAIKALD"
BASE COUNT          811 a         1619 c         1952 g          835 t
ORIGIN      
        1 catcgagggg tacatcgtca gcatgtcttc gtcgggttcg acccagtcgg actcagcgaa
       61 ggcattgagg aggttcggga tgagcgtgac gaggtcgtag agcgcggcag ccacctcggc
      121 ggagtcctgc acgagggcgc ccggctgctc cacgatcttg agggcgttca ggccctggcg
      181 gaggaggtcg ctcagctcgc ggggccagag catacggtcg gggcggccga gggaggagcg
      241 caggaggttc tcgcagaagg ccgcgcgcag agccatcgtg cggacgtcgg ggcggcgctc
      301 ggcctcgaag gcctggaggc gctgcagcca gacgcggatg ccgccgtact cgcgggcgac
      361 gtaggcgtcg atgcgcgtgt cctcgacgat ggtgaacagg gcggcgatca ggtcgcggtc
      421 ctcgaagagg tcgtagaagc gctccatcga ggtgatgacg ggtcgctcgc gggccgggag
      481 ctgcgcctcg cggtcgcgca cggtggcggg cgtgtgcacg ccgggctcgc cgaagcggaa
      541 ggcgaacgag ccgaactcaa tgcggccggt ctggtgggtc gtgaagacct tgtagcactg
      601 gaagttggcc gcctggtcgg ggaaggagcc cacgaacgga ggcaggtaga tcgaggtgcc
      661 ctcggtggtc gcgacggact ccgtgaccca gccgatgccc ttatcgacga gttcctcggc
      721 ggagcgaatg gcgacctgct cgccggtgag ggccttggca tacatgcgga gcagcgagcc
      781 gaccgtggtc agttcgacgc gcgcggagag cgccgcgagc gcctgctcgg cgcgcaccga
      841 ctcgaggcgg aagtacgcct cggcgccctc gatgttgcct tcctcgaaga ggaggcggct
      901 gccgggctcg gcccactgcc caagctggtc ggtcgtgagg cgggtgagca cggacggcgc
      961 cgaggagagc agggacatcg cggcgaccgg gctgcccttc gcgatatgcg tggcgagtgc
     1021 aatcaggacc tggtgctcgg cggtggggac ggtcgcgagg gccgtcgcgg catcgacgaa
     1081 gtgggggaag ccctggcggc cgcccgcagt gacgaccttg gcggcgatat cgaggaacgt
     1141 ctcgcggtcg tcctgctcga cgccctggat gagctgcggg ccgcgctcga agtagaggcg
     1201 ggtgtcagcc caggaggcgc gcgtcacggc gcgggcgaaa cgcaggaagg gctcgcgatc
     1261 cgccggacgc aggccggcga acaactcggg cgagctttcg aggcagatgg tcgcgagctg
     1321 gtaggaacgg tccgagagca ggtcgatgac ctcgacgagc tcctccacga ggtggaggcg
     1381 gagcgggtcg agcaacctcg gcgtcagctc gtaaaacagc gaggccagcg cgacggactt
     1441 ccagttcccg cggtgcaggc gctcgcccat gcgagcccag cgaccgatct cgccctggcc
     1501 ggcgtggcgg agcgggatct cctcgcccat gatgccgacg ctgtcgccgc gctccagcgg
     1561 gagcagcggg atgacctcgg gggcggcgcg gaggaacgcg gcggcgacgg gcggtgagcc
     1621 ctccgtaatg cggcctgcgg tctcggcgca gtacatgacg agattgaagg gcagctggtt
     1681 cgcgatacga gggccgacgc ggaagtactc gacggcggcc tcccaagagc gcaatgagcg
     1741 gccggcgagg tcgacgccgg cagcggccca tgcctcgaga tcctcagggg agaggatcgg
     1801 ctccatggag cggagggcga ccgcgaactc ctcgggcacg gcggggccat gtccgcggag
     1861 ggcggggtcg tggcgggcca tcagttcttc gacaatgctc atgaggtcac gctattcggc
     1921 ttgtgcaccg ggtcatcgta cacgtcgaac gtcacctcgc cactctgcgt gtcgaggatg
     1981 gcgtaggagg aacggaagcc gttccaggga tcgcgcggct ggcccgcgga gcccgggttg
     2041 atgacgaggg tgccgttcac ctggtgggtc atcgcgcaat gggtgtggcc gtggatgacg
     2101 aagtcggcac cgaggttgcc gaactccttc atcacggcgt tgtgcgggta gacgtattcg
     2161 ccgcagtcgc ggtgcgagcc gtggaacatc gtgatgcgct tgccatcgat gacggtgtcg
     2221 atgcgcttcg gccgctcgga gagccacttg aggaggtcct ggtcgacgtc gggctgctcg
     2281 cggacgcgct gaccgccgcg gctgaggacg acttcctcgt ggttgccgag cacgacgtgg
     2341 gcgccgatct ccttgaggcg accgatcacc tcgttggaga agcggaactg gaagatggcg
     2401 tcgccggcgc acagcacttc gtcgacatgg cccatccggg agagggccat gtcgagcgaa
     2461 gcgatgttgc agtggatgtc agagacgatt ccgagtcgca ccggacctcc cgtccgtgtc
     2521 cgcccgtcag tcggcgaaga tcgaggtcac gacctcctcg agggagcgct ggacctcgag
     2581 gtcgtcggtc aggccccaca cgactgcgga ctggcacgca cggcgcggcg cgatgccact
     2641 ggcgatcagt ttgccggcgt acaccagcag acgggtggag acgccctcgg cgaggccgtg
     2701 ctccttcagg ttgcggacct tctccgcgag cttggccagc tcattggcca tctcctggga
     2761 gcagcccgac tccgtgcgga tgatctccgc ctcctggtca cgcggcgggt agtcgaactc
     2821 gatggcaatg aagcgctgcc gggtcgagtg cttgaggtct ttcagcgccg actggtagcc
     2881 ggggttgtag gacaacacca gcaggaagcc cggagccgcc tcgaggaggt cgccgcgctt
     2941 gtcgatcggc agcaggcgcc ggtagtcggt gagggggtgg atgaggacgg tggtgtcctt
     3001 gcgcgcctcg acgatctcgt cgaggtagca gatggcgcca accttgacgg cccgggtgat
     3061 ggggccgtcg atccagcgcg tctcctcgcc ctcgaggagg tagcggccga cgaggtcgga
     3121 ggccgtgagg tcctcgtgac aggcgacggt gatcaggggc acgccctcgt cgtcgcggct
     3181 gccgaggtgg ccatgcaggc gccacgacat gtactcgacg aagcgagtct tgccgcagcc
     3241 cgtggggccc ttgaggagca ccggcacgtt ctggtcgtag gccgtctcga acagctggac
     3301 ttcgtccgca atggggaggt agtacggctc actggtgagc cggaagtcct cgatctgcat
     3361 ctgcttgaag gcctgttcgg ccatcggttg agtcgtggtc acttgcgcgc cgcccgcttc
     3421 gttcccttgt ccgccgcacc tgctttggcg ttggcggcgt tggcttcatc gacccgcttg
     3481 agcagcttct tccactcgcg ctcgacgcgc tgttcgaact gctcgagggt gctggaattg
     3541 tcgaccttca cgtcggcgta ggccaaacgc tccttgtttg acagctgcga gtcgatgcgg
     3601 gcctgtgcct gcgcctcggt cagtccgttg cgcgccatca ggcgctcgcg ggcgacctcg
     3661 gggctgaccg tgacgaccca cacctcgtcg gcgaggtcca tccagttggc ctcgatcatg
     3721 accgcagcct cgaggacgat cgtcgaggcg gggtcgcgct tcttcaggtc cgcgatctcc
     3781 tcggcggcga gcttgcggat ctcgggccag acgatgtcgg tgagccgctt catctgggcc
     3841 ggatcgccga agaccttgcc gccgagcagc tgtcgattaa tcgtaccgtc cgcggcaacg
     3901 aggtcgtgcc cgaactcgtt gaccacggcc tgaaagccgc tggtccccgg ctcgtagacg
     3961 cggtgcccga cgcggtcggc gtcgatgaca atggcgccat agcccttgag caaactcgcg
     4021 gcagtagact tcccggaggc gatccctccg gtcagaccaa taacggtcat gtaaggctcc
     4081 gtaggtaccc gaaaaccagc gttcggctgt agcaatcccg attgtattgg caccccagta
     4141 cgggtgcaag gtgaagcggg actgagccaa ctccggttta tgtcactcgt tgacctcgga
     4201 ccgctgatac gcgagagggc cggtgcgacc ggccctctcg gtgcttgcgg tctgtttccg
     4261 cggggctagt cgagcgcctt gatggcggag atcagctccg ggaggatcgc cttgtagtcg
     4321 cccacgatgc cgaagcgggc ggacttgaag atgttcgctt ccgggtcgcg gttgatggcg
     4381 acgatggtct tcgagccgct gcaccccgcc atgtgctggc tggcgccgga gatggcgacg
     4441 gccacgtaga ggtcggggct gacgatcttg ccggtgagcc cgacctgctg gctcggcggg
     4501 taccagccga ggtcgcacgc ggcgcgtgag gcgccgacag cagccttgtc ggagccgaag
     4561 gcggcggcga gctcctcgat gacctggaag ccctcgggcg cgccgaggcc acggccacca
     4621 gagacgacaa tcggggcgtc ctggatctgg aggccttcgc tgaccgcggc ctcgcggccg
     4681 gtgatctccg tgcgggacgc acccgcggcc ggcaactcga ccgtctcacc cgaggcgccg
     4741 gctcgcggct cgatcgggtc ctgcgacttg gcgcggacgg tcacgaccgc cggcgaggtc
     4801 gcaaaggagt aggtggcgtg ggcgttccca ccgtaggcgg aacgagtcgc gcgcagactg
     4861 ccgtcgacgt tttcgacacg gaggcagtcc atcgcgaccg cggtgttgcg gcggaaggcg
     4921 aggcgagggc cgaggtcgcg gccgacgccc gactgcgcga tgagcacgac gccggcgctc
     4981 gtctggtcga cggcggcctc gacggcggag agccactggt cggagcggaa gtcgtcgtac
     5041 ccggagtcgc tggagacgca ggcgcggtcc gcacccgcgg cgaaggcgtc agcggcggcc
     5101 ccggccgcgg cgctgccgag gagcgtgacg ctgaccttgc cgccgtcggc gacgcggggg
     5161 gctgcgccga gcagttcggc ggtgacgggc gagagcttgc cgtcggcgag ctcgccg
//
LOCUS       DAORPG010000055         5212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_989_length_5212_cov_3.762653, whole genome shotgun sequence.
ACCESSION   DAORPG010000055 DAORPG010000000
VERSION     DAORPG010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_989_length_5212_cov_3.762653"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..1151
                     /locus_tag="PLX85_02095"
     CDS             240..1151
                     /locus_tag="PLX85_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC61999.1"
                     /translation="MFDRIHHAGLAVADLVTAKAVFADALGLRVDTTRSPYPEGNKQR
                     GADPTDILDIPIGNAEIELNAAPRDGTPAGGTHRFVEARGGVGALHHICLHSDNVAED
                     VAHLRASGITQIAASPEQLGSDEPWTQVAFFHPKDCLGVLLEIWTPDNHRVGDRYQGA
                     GVFTRLHHIGVVTNDLEAARHLWCNVMGFRVDPFRSPVNQGGRFVESDGVRVLNIPIG
                     RDGGEIVAVLPQRAGSGTARFLERYGGRSNGTMHHIALATRDVRAAADFVRDRGLEIV
                     GDIDEDFAWIHPEKAGGVLIQIVRETR"
     gene            complement(1175..2464)
                     /locus_tag="PLX85_02100"
     CDS             complement(1175..2464)
                     /locus_tag="PLX85_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62000.1"
                     /translation="MRPSGNLDGSGPLYRPVSRRGFLEAATTIAVAAPLLAHPIIPGV
                     RNLDMDGDPRLRAEARLPLEGFLEHYAALIRERRIRIGCSFSPESLGVDGPRVGERGK
                     ARALRVLQAAVEDLGMADIRLGLRWSNLAPNGADLTTFYAPYLDYCFRNASVRTVALD
                     FGPLKTFRWPEIHVPQPVLDGLDSVPGDTAEIRPRMAVAQRGLEHLARAVAFVGQEYD
                     GAKPVTISLNEPFHGFGRFSWTMSEAYLLEVLDVVFGSGHFADAGVLVNSAQGLDLHR
                     IAAFFQQLVRQRPELRGRLTSGFDIYPFLPPMSHFPVLKQLLGNIRAHKRDWSQQVGP
                     NLEWAHDPEAGYRIEVTEAQAEPFGDHPLVGNSLPHFQHVLAECFDKILDPEQDESLI
                     RLFGIEYQLRLQLSGRAAPDNLAILELTRRINELGAR"
     gene            2609..3079
                     /locus_tag="PLX85_02105"
     CDS             2609..3079
                     /locus_tag="PLX85_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62001.1"
                     /translation="MQQYTDRLRQRYVHEPVQWGTLAGLLLMVIGVFGPWATVSIKVL
                     GISASTNGMDHKGDIVFIIALAAAAAIVAYAFLNVRIEERMLMWGLVAAALIIDLLVL
                     LDFLDIMGTDGVGIGWGLWLTVVGAAAFTAGAVVPMWSEIRAKASDMTERGQGA"
     gene            3279..4511
                     /locus_tag="PLX85_02110"
     CDS             3279..4511
                     /locus_tag="PLX85_02110"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006541776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRC62002.1"
                     /translation="MKALERIRITDFSGVLAGAGASKILAAFGAEVIRVENPSNLGAW
                     DVVRGSPPYIDERRGHNLGGGFNNHNAGKLGVTLNLQTEKGRAILPRLIAISDVVAEN
                     FAAGVMERLGFGYDRLREIKPDIIYLSNCGFGASGPYVSFKTWGPIVQAVSGLTHISG
                     LPGLPPAGWGYSYMDHTGAYYGAIAMLAALHHRNRTGEGQWIDLSMTEAAASLTGTAI
                     LDWTVNGRPARRDGYPDANHSDSPPMAPHGIYPARDEDHWVAIACRDDADWQAIAEEI
                     AELWVHDARFATLEGRLANQPALDELMSRWTRLFDEDEVAERLQAAGVPAAPVQRPEQ
                     RIDQDPNTAAWGLWPEVKHTEMGHVRVEGMAAHLSKTDWSLDRAAPCLGEHNREVYGG
                     LLGMSDDEIDQLQAEGVI"
     gene            4508..>5212
                     /locus_tag="PLX85_02115"
     CDS             4508..>5212
                     /locus_tag="PLX85_02115"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRC62003.1"
                     /translation="MTGPLTDLRVIEYCDEAGAWAGKLFADMGADVVKVEPPGGSRVR
                     GYPPFLGDEPHPDRSLAFWHNNTSKRAITLDLEQEAGREVFRRLAAGAAIVLEDQSPG
                     QMAELGLDYPDLSALNPALIMVSITPYGRSGPKSDWVATDLTVLAGGPEWSNGYDDHS
                     LPPVRGLGNQGYQTACHWAVATALVAVLHRDWTGEGQHVDVNMHAGANVTTEASSYTW
                     LVARQTVQRQTGRHATV"
BASE COUNT          864 a         1900 c         1657 g          791 t
ORIGIN      
        1 gccgccagct cccgccactc cacgaacgag ctcgcgagcg gcccgccgtg ttgcagtcgt
       61 tcgatcgccg tcagtaccgt catgtgttcc tcgccatcgt tagaacacat gaactatagg
      121 tacgtacgtc tgttcttgtc acgctccctg cgacgcacgc tcgacagctt cgagggccgc
      181 tggtacatct ctgccatcac gaatcgcgtg gcaccacgcg tcgagtcagg agcagcacct
      241 tgttcgatcg gattcaccac gccgggctcg ccgtcgccga cctcgtcacc gccaaggccg
      301 tcttcgccga cgcgctcggt ttgcgcgtcg acaccacccg ctcgccctac cccgagggca
      361 acaagcagcg cggcgccgac cccaccgaca tcctcgacat cccgatcggc aacgccgaga
      421 tcgagctgaa cgccgccccg cgcgatggca cgcccgctgg cggcacccac cgcttcgtcg
      481 aggcgcgcgg tggcgtcggt gccctgcacc acatctgcct gcactccgac aacgtcgccg
      541 aggacgttgc gcacctccgc gcctcgggca tcactcagat cgccgcgtcc cctgagcagc
      601 tcggctccga cgagccgtgg acgcaggtcg cgttcttcca cccaaaggac tgcctcggcg
      661 tcctcctcga gatctggacg ccggacaacc accgcgtcgg cgaccgctat cagggcgcgg
      721 gcgttttcac ccgcctccac cacatcggag tcgtcacgaa cgacctcgag gccgcgcgcc
      781 acctctggtg caacgtcatg ggcttccgcg tcgacccgtt ccgctcaccg gtcaaccagg
      841 gcggtcgctt cgtggagtcc gatggggtac gcgtcctcaa catcccgatc ggtcgcgacg
      901 gcggcgagat cgttgccgtg ctcccccagc gcgccggcag cggcaccgcc cgcttcctcg
      961 aacgctacgg cggacgcagc aacggcacga tgcaccacat cgccctcgcg acccgtgacg
     1021 tgcgcgccgc cgccgacttc gtccgcgacc gcggcctcga gatcgtcggc gacatcgacg
     1081 aggactttgc ctggattcac ccggagaagg ccggcggcgt cctcatccag atcgtccgcg
     1141 agacgcggta gtggcggtga gcgaaggctc ggctctacct cgccccgagc tcgttgatcc
     1201 gccgcgtcaa ctcgaggatc gcgaggttgt ccggcgcggc gcgcccggat agctgcagtc
     1261 gcagttggta ctcgatgcca aagagtcgga tcagcgattc gtcctgctct gggtcgagaa
     1321 tcttgtcgaa gcactccgcg aggacgtgct ggaaatgcgg caacgagtta ccgaccagcg
     1381 ggtggtcgcc gaatggctct gcctgggctt ccgtcacttc gatgcgatag ccggcctcgg
     1441 gatcgtgcgc ccattcgagg ttcggcccaa cctgctgtga ccagtcgcgc ttgtgggcac
     1501 ggatattccc aagcagttgc ttgaggacgg ggaagtgaga catcggaggc aggaacggat
     1561 agatgtcgaa gccgctcgtg agccgtccgc gcagctccgg ccgctgacgc acgagctgct
     1621 gaaagaacgc ggcaatgcga tgcaggtcaa gcccctgcgc cgaattcacc agcacgcccg
     1681 catcggcgaa atggcccgag ccgaacacga cgtcgagcac ctcgaggaga tacgcctcac
     1741 tcatcgtcca gctgaagcgg ccgaacccgt gaaacggctc gttgaggctg atcgtcaccg
     1801 gcttcgcgcc gtcgtactcc tgccccacga acgccaccgc gcgtgcgagg tgctcgaggc
     1861 cccgctgcgc gaccgccatt cgcggccgga tctccgccgt atccccgggc accgagtcga
     1921 gaccgtcgag gaccggctgg ggtacgtgga tctccggcca gcggaacgtc ttcagcggcc
     1981 cgaagtccag cgcgacggta cgcaccgagg cattccggaa gcagtagtcg aggtacggcg
     2041 catagaacgt cgttaggtcg gcgccgttcg gcgcgaggtt gctccaccgc agcccgaggc
     2101 gaatgtcggc catgccgagg tcttcgacgg ccgcctgcag cacgcgcagc gcgcgtgcct
     2161 tgccgcgttc gccgacccgt ggcccgtcga ccccgagcga ttcgggggag aaggagcagc
     2221 cgatgcggat ccgtcgctcg cggatcaacg ccgcgtaatg ctcgaggaac ccttcgaggg
     2281 gcagtcgtgc ctcagcccgc aggcggggat cgccgtccat atcgagattc cgaactccag
     2341 ggatgatcgg gtgtgccagc aacggcgcgg cgaccgcgat cgtggtcgct gcctcgagaa
     2401 agccgcgccg cgacacaggc cggtagagag ggccgctgcc gtcgaggttg ccggatggtc
     2461 gcacggtgat accagctgac taaggagcgg ctggcgaggt caatactcgc ctcctaagga
     2521 taggcggtcg gccctcacaa cagggcatca cgtctgtaca atccgcccat acagcggcgg
     2581 gcatggagcg atgccagagg aggcctcgat gcagcagtac acagaccggc ttcgtcagcg
     2641 ctacgtgcat gagcccgtgc aatggggcac gctcgcgggc ctgctcctca tggtcatcgg
     2701 cgtcttcggg ccctgggcca ccgtttcgat caaggtgctc ggaatcagcg cctcgacgaa
     2761 cggaatggat cacaagggcg acatcgtctt catcatcgcg ctggcggccg ccgccgccat
     2821 cgtcgcctac gcgttcctca acgtccgcat cgaggagcgc atgttgatgt gggggctcgt
     2881 cgccgccgca ctgatcatcg atctcctcgt cctgctcgat ttcctcgaca tcatgggcac
     2941 ggacggcgtt ggcatcggct ggggcctgtg gctcacggtg gtcggggctg cggcgttcac
     3001 cgccggcgcg gtcgtgccga tgtggagcga gatccgcgcc aaggccagcg acatgaccga
     3061 gcgcggtcag ggcgcctagc caggccgccg ggagttcctc cggagcccgt ccggcggacg
     3121 ggctccaccg tcgtccccgt cgaagacacc cgtctcgaga gcgcacgcgt ggccgtcctc
     3181 gacgtccgcc gtgatcgccc gcgaggcgag tacggcgccg cgcaccatcg cggcgcgttc
     3241 ggtatgctgc cgcccacatc gatgctcggg aggcaaccgt gaaggcactc gaacgcatcc
     3301 gcatcactga cttctcggga gtcctcgccg gggccggcgc gagcaaaatc ctcgcggcct
     3361 tcggcgccga ggtcatccgt gtcgagaacc cctccaacct cggggcgtgg gacgtcgtcc
     3421 gcggctcgcc gccctacatc gacgagcgcc gaggacacaa cctcggcggc ggcttcaaca
     3481 accacaacgc cggcaagctc ggcgtcaccc tcaaccttca gaccgagaag gggcgcgcga
     3541 tccttccgcg cctcatcgcg atctccgatg tcgtcgccga gaacttcgcc gccggcgtca
     3601 tggaacgcct tggcttcggt tacgaccgtc tgcgcgagat caagccggac atcatctacc
     3661 tctcgaactg cggcttcggc gcctccggcc cctacgtgtc gttcaagacc tggggcccga
     3721 tcgtgcaggc ggtgagcggc ctcacgcaca tctcgggcct tccgggcctg ccgcccgccg
     3781 gttggggcta ctcgtacatg gatcacacgg gcgcctacta cggcgccatc gcgatgctcg
     3841 cggcgctcca ccaccgcaac cgcaccggcg agggccagtg gatcgacctt tcgatgaccg
     3901 aggcggcggc gagcctcacg ggcacggcca tcctggactg gaccgtcaac ggtcgccccg
     3961 cccgccgcga cggatacccg gacgccaacc attcggactc cccgcccatg gccccgcacg
     4021 gcatctaccc cgcacgtgac gaggaccact gggtcgccat cgcctgccgc gacgatgccg
     4081 actggcaggc catcgcagaa gagatcgccg agctctgggt gcatgacgcg cgtttcgcca
     4141 ccctcgaggg ccggctcgcc aatcagcccg cgctcgacga gctcatgtcc cgctggacgc
     4201 ggctcttcga cgaagacgag gtcgccgaac gcctccaggc cgcgggcgtc cccgccgccc
     4261 cggtgcagcg ccccgagcag cgcatcgacc aggaccccaa caccgcggcg tggggcctct
     4321 ggcccgaggt caaacacacc gagatgggcc atgtccgcgt cgagggcatg gccgcccacc
     4381 tctcgaaaac cgactggtcg ctggaccgtg ccgcgccctg cctcggagag cacaaccgcg
     4441 aggtctacgg cgggctgctc ggaatgagcg acgacgagat cgaccagctg caggcggagg
     4501 gggtgatatg accgggccgc tgaccgacct ccgcgtcatc gagtactgcg acgaggccgg
     4561 cgcctgggcg ggcaagttgt tcgccgacat gggcgccgat gtcgtaaagg tcgaaccgcc
     4621 cgggggcagc cgcgtccgcg gctacccgcc gttcctcggc gacgagcccc accccgaccg
     4681 cagcctcgcc ttctggcaca acaacaccag caagcgcgcc atcaccctgg atctcgagca
     4741 ggaagccggc cgcgaggtct tccgccgcct ggcagccggc gcggcaatcg tcctcgaaga
     4801 tcagtcgccc ggccagatgg ccgagctcgg cctcgactac cccgacctgt cggcgctcaa
     4861 ccccgccctc atcatggtct cgatcacccc ttacggccgc agcggtccca agtcggactg
     4921 ggtggcgacc gatctcaccg tcctcgccgg cggcccggaa tggtcgaacg gctacgacga
     4981 ccactcactg ccgcccgtcc gagggcttgg caaccagggt taccagaccg cctgtcactg
     5041 ggccgtagcc accgccctgg tcgcggtcct ccaccgcgac tggaccggcg aaggccagca
     5101 cgtcgacgtg aacatgcacg ccggcgcgaa cgtaactacc gaggcctcct cgtacacctg
     5161 gctcgtggcg cgtcagaccg tgcagcgcca gaccggccgc cacgccaccg tc
//
LOCUS       DAORPG010000056         5151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1014_length_5151_cov_3.287284, whole genome shotgun sequence.
ACCESSION   DAORPG010000056 DAORPG010000000
VERSION     DAORPG010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1014_length_5151_cov_3.287284"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PLX85_02120"
     CDS             <1..528
                     /locus_tag="PLX85_02120"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRC62004.1"
                     /translation="LVRLRAGADSHAVADRLIAATDGQFDVEVLSDTVAALRDSWRPL
                     LLGLNAVLFAVAGVNLTSNLLLGVRERRRELAILKTVGFTPRQLVLSVLSGGAGIALL
                     ATLLGIPLGLVATRLMFDALSSTAGIGTGIGALPGVRWLALLVPIAVGTTALASLFPA
                     TTAARIRVAEALRYE"
     gene            complement(570..1346)
                     /locus_tag="PLX85_02125"
     CDS             complement(570..1346)
                     /locus_tag="PLX85_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRC62005.1"
                     /translation="MAELRVEISDYIAVVTMDNPPVNAFSMDWNFQETFDELGDRDDV
                     RVAILTGAGDRIFCAGADVRRAAANLDAGPPRPGEGTTSSRRSRENFYVVTECAVPVI
                     GAINGPALGAGLALAASCDYLIASERAVFGLPEIDVGLLGGARHFMRLFGNQQTRRAN
                     FTGIRLDAQEAYRLGAVIKVVPPEQLMEVALEEARIIASKMPVGIKMAKAALNMIEDM
                     DLRNGYRFEQTQTAILVKTEDAQEAKRAFAEKRPPVFRGR"
     gene            1491..1574
                     /locus_tag="PLX85_02130"
     tRNA            1491..1574
                     /locus_tag="PLX85_02130"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1524..1526,aa:Leu,seq:taa)
     gene            complement(1606..2922)
                     /locus_tag="PLX85_02135"
     CDS             complement(1606..2922)
                     /locus_tag="PLX85_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRC62006.1"
                     /translation="MPAPRRQPAQPSLFPDPLAEPRADAPLAARMRPRDLDEFVGQER
                     LLGPGRPLRLLIERDEVPSLILWGPPGSGKTTLASVIARRTRRHFTRLSAVSSGVADV
                     RQAVTDAEQRLRANGVGTILFIDEIHRFNRGQQDALLPHVEDGTVTLIGATTENPSFA
                     VVAPLLSRSRVVRLEALGSEEIARIVARALDDRERGLGDARLSIDDDALAAIAAFAAG
                     DARKALAVIEVAAGLAEVDAAGRRVITPATVEAAAQQRTLHYDRAGDAHYDTISAYIK
                     SVRGSDVDASLYWLARMLEAGEDPLFVVRRMVILASEDVGLADPAALQLAVAAQQAVH
                     FLGMPEAALPLAEVTVYLARAPKSNSAYAAYQRALADVEGSRNDPVPLHLRNAVTGLM
                     RNLGYGAGYQYAHDYAGHVPPAQTHRPPSVEGHRYYEPGTLGDESR"
     gene            complement(3002..3205)
                     /locus_tag="PLX85_02140"
     CDS             complement(3002..3205)
                     /locus_tag="PLX85_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62007.1"
                     /translation="MKWYFDPEQRLYWSLGDGDDEIFLRQDFVQGNEMAAIMSGVSLD
                     GIEHAEVKPVPRLPADHEVRGAS"
     gene            3294..4121
                     /locus_tag="PLX85_02145"
     CDS             3294..4121
                     /locus_tag="PLX85_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005992133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRC62008.1"
                     /translation="MLEYAALAVLGVVVGAFGTLVGAGGGFLLVPVLLFLYPDRDADT
                     ITAMSLLVVLANAASGSAAYAWQRRVDFHSGGWFALATLPGSVAGALLVGFVPRQAFD
                     IVFALVLAGVGAFLLTPRRGVTAVRDPLTGPGVVRRMMRDRDGHTYVYAYRRWQGLAI
                     SLGVGFVSSLLGIGGGIVHVPAMAIVLHFPVHIAAATSHFVLAVTALEATGTHVLAGS
                     LRGEPMAQALAIALGAVGGAQVGARLSTRVHGPLIIRALGAALVLVALRLGAAAIWA"
     gene            complement(4197..>5151)
                     /locus_tag="PLX85_02150"
     CDS             complement(4197..>5151)
                     /locus_tag="PLX85_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC62009.1"
                     /translation="KLHLDGMVYKIMPDGTARTAAFRAGQVEYAYALAGKLSDVKNIV
                     GTNPNVQVFMVNPTAGGYGFGMNVSNPKFKDERVRRAISLSQDHDTTIQLVFEGYGVS
                     APDQPFTFLFNKMPSYKAGEIGPWVKATGDPAQAKQLLDAAGLKDFTINATYYTYGQY
                     DADRPQVLTDQMRKAGITLNAKRVEYTEFNSAWTTAKLEEATTSGWGAPGFDADNYFY
                     NQLYSKSPGNRHQINDPQIDEWAQQQRTELDPAKRKDIQMKIWNRVWMDQMYRIPQAA
                     GYTYEVMQPWLRAWRGGGGPVGTSSYFYDWGEQLIDIWLDK"
BASE COUNT          722 a         1725 c         1799 g          905 t
ORIGIN      
        1 ctcgtacggt tgcgcgcggg agccgacagc cacgcggtgg ccgaccggct gatcgcggcg
       61 accgatggcc agttcgatgt cgaggtgttg tctgacacgg tggctgcatt gcgcgatagc
      121 tggcgtccgt tgctgctggg cctcaacgcc gtcctcttcg ccgtggccgg cgtgaacctg
      181 acttcgaacc tcctgctcgg cgtccgtgaa cggcggcggg agctggcgat cctgaagacg
      241 gtcgggttca cgccccgcca gctggtgctg tctgtcctct cgggtggcgc aggcatcgcg
      301 ctgctcgcga cgctgctcgg catcccgctc ggactcgtcg ccacccggct catgttcgac
      361 gcgctgtcga gcaccgccgg gatcggcacc ggcatcggcg cactcccggg ggtgcgatgg
      421 ctggcgctgc tcgtcccaat cgccgtgggc acgaccgcgc tcgccagcct gttccctgcc
      481 accaccgcgg cgcgcattcg ggtcgccgag gcgttgcgtt acgaatgaac gaagaggcct
      541 ccctcgcggg aggcctcttt ctgacggggt cagcggccgc gaaacacggg cggacgcttt
      601 tcggcgaatg cccgcttggc ttcctgcgcg tcctcggtct taacgaggat cgccgtctgg
      661 gtctgttcga agcggtagcc gttgcggagg tccatgtcct cgatcatgtt gagggcggcc
      721 ttggccatct tgatgccgac cggcatcttc gaggcaatga tgcgtgcctc ctcgagggcc
      781 acttccatca gctgctccgg tggcacgact ttgatgacgg cgccgagccg gtaggcctcc
      841 tgagcgtcga ggcgaatgcc cgtgaagttc gcacggcgag tctgctggtt gccgaatagc
      901 cgcatgaagt gccgggcgcc gccgaggagc ccgacatcga tctccggcag gccgaagacg
      961 gctcgctccg aggcgatcag gtagtcgcag gacgccgcga gtgcgagacc cgcgcccagg
     1021 gcgggcccgt tgatcgcgcc gatgaccggc acggcgcact ccgtcacgac gtagaagttc
     1081 tcgcgcgagc gccgtgagct ggtcgtcccc tcgcccggcc gcggtggacc ggcgtcgagg
     1141 ttcgcggcgg cccgccgcac gtccgcgccg gcgcagaaga tgcggtctcc ggcgcccgtg
     1201 agaatggcca cgcgcacatc gtcgcggtcg cccagctcgt cgaaggtctc ctggaagttc
     1261 cagtccatgc tgaaggcatt caccgggggg ttatccatcg tgaccaccgc gatgtagtca
     1321 ctgatctcga cgcgtagctc ggccatgtcg tctccctacc tcgtcgtccc cgacgggcat
     1381 cgatcgacgg cattaaacac cctgcaggca agcgccgtcg cctcgcgagc ggcgagagga
     1441 gcagctaccc tcgtgtgata caggcgaccg cgtcgatggt cgcgtcgagc gccggggtgg
     1501 cggaacggca gacgcagcgg acttaaactc cgcggccgca aggcgtgagg gttcgagtcc
     1561 ctcccccggt accagagcca gcgtcctatt gctcctcgcg aggctctaac ggctctcgtc
     1621 ccccagtgtg ccgggttcgt agtagcggtg cccttccacg gacggcgggc ggtgtgtctg
     1681 agcgggcggc acgtggccgg cgtagtcgtg ggcgtactga taaccggccc cgtagccgag
     1741 gttgcgcatg aggccggtca ccgcgttcct cagatggagc ggcacgggat cgttgcgcga
     1801 cccttcgacg tcagcaagcg cgcgctggta ggcggcgtac gcggagttcg acttcggagc
     1861 gcgggccagg tacacggtca cctccgcgag cggaagcgcc gcttccggca ttccgaggaa
     1921 atgcacggcc tgttgcgccg caaccgccag ctgcaacgcg gcgggatcgg cgagcccgac
     1981 atcctcggac gccaggatga ccatgcgccg gaccacgaag agcgggtcct cgcctgcctc
     2041 gagcatgcgc gcgagccagt acagcgacgc gtcgacgtcc gaaccacgga ccgacttgat
     2101 gtaggccgaa atcgtgtcgt aatgcgcgtc gcccgcgcgg tcgtagtgca gggtgcgttg
     2161 ctgcgccgct gcttcgacgg tcgctggcgt gatgacgcgt cgtccggccg catccacctc
     2221 cgccaggccg gccgcaacct cgatcaccgc cagcgccttg cgcgcatcgc cggctgcgaa
     2281 ggcggcgatg gctgcgagcg cgtcatcgtc gatcgacaag cgcgcgtcac ccaggccgcg
     2341 ctcgcggtcg tcgagggcgc gcgcgacgat gcgagcgatc tcctccgagc ccagcgcctc
     2401 gaggcgcacg acccggctgc gggagagcag cggtgcgacg accgcgaacg atgggttctc
     2461 ggtcgtggcg ccgatgaggg tgacggtccc gtcctcgaca tgggggagca gcgcatcctg
     2521 ctgaccacgg ttgaagcggt ggatctcgtc gatgaacagg atggtgccga caccatttgc
     2581 acgcaggcgc tgttcggcgt cggtcaccgc ctggcggacg tcggcgacgc ccgacgagac
     2641 cgcagacagc cgtgtgaagt gccgccgggt gcgccgcgcg atcaccgagg cgagcgtggt
     2701 cttgccggac ccaggaggcc cccacaggat cagcgatggc acctcgtcgc gctcgatgag
     2761 caaccgcagc ggccggccgg gcccgagcag gcgttcctgt cccacgaact catccaggtc
     2821 gcgcggacgc atccgagcgg cgaggggcgc atccgcccgt ggttccgcga gcgggtcagg
     2881 gaacagggat ggttgcgctg gctggcgacg tggcgcgggc acgagccgag tgtaggcgat
     2941 ccccgaacac aagcgtgccc ccggtttctc gggggcacgt gcggcgtgac tgcctcgcag
     3001 gctagctggc gccgcgtacc tcgtggtcgg cggggaggcg cgggactggc ttcacttcgg
     3061 cgtgctcgat gccgtcgagg ctgacgccgg acatgatggc cgccatctca ttgccctgga
     3121 cgaagtcctg acgaaggaag atttcgtcgt cgccatcgcc cagagaccag tacagacgct
     3181 gttcgggatc gaagtaccat ttcatggccg cgtactatac cacctgagcg aattcctcgt
     3241 gcgctgcgac acgaacggca gccgcggctc ggcccgaatg acgggaggcc ggcttgcttg
     3301 agtacgccgc gctcgcggtc ctgggcgtgg tggtgggagc attcggcacg ctcgtgggcg
     3361 ccggtggcgg gttcctgctc gtcccggtgt tgctgtttct ctaccccgac cgcgacgccg
     3421 acacgattac cgccatgtcg ctactcgtgg tgctcgcgaa cgcggcctcg ggttcggccg
     3481 cgtatgcctg gcaacggcgg gtcgatttcc acagcggtgg ttggttcgcg ctggcgacgc
     3541 tgccgggttc cgtagcgggc gccctccttg tcgggtttgt cccgcggcag gcgttcgaca
     3601 tcgtgttcgc gctcgtcctg gcgggggtgg gagccttcct gctcacccct cgtcgaggcg
     3661 tgaccgcggt gcgggacccg ctcaccggac cgggggtcgt gcgcaggatg atgcgcgacc
     3721 gcgacggcca cacgtacgtc tacgcctacc ggcgctggca ggggttggcc atcagcctcg
     3781 gcgtcggctt cgtctccagc ctgctcggca tcggcggcgg catcgtgcac gtcccggcga
     3841 tggcgatcgt gctgcacttc ccggtgcaca tcgcggcggc cacgagccac ttcgtgctgg
     3901 cagtaacggc cctcgaggcg accgggacgc acgtccttgc gggttcgctc cgtggcgagc
     3961 cgatggcgca ggcgcttgcg atcgcactcg gcgccgtggg cggggcacag gtcggcgccc
     4021 ggctctcaac gcgcgtgcac ggcccgctga tcatccgcgc gctcggcgcg gcgctcgtcc
     4081 tcgtagcgct ccgtctcggt gccgcggcga tctgggcgtg aggatgcaaa acgccctcgg
     4141 actttcgtcc gagggcgctc ggtctggccg ctggagcgac cggatacgcg gggtggttac
     4201 ttgtcgagcc agatatcgat gagctgctca ccccagtcgt agaagtagct gctcgtaccg
     4261 acgggaccgc cgccgccacg ccaggcgcgc agccagggct gcatcacctc gtacgtgtat
     4321 cccgcggcct gcgggatgcg gtacatctgg tccatccaga cgcggttcca gatcttcatc
     4381 tggatgtcct tgcgcttggc cgggtccagt tcggtgcgct gctgctgcgc ccactcgtcg
     4441 atctgcgggt cgttgatctg gtgtcggtta cccggcgact tcgagtagag ctggttgtag
     4501 aagtagttgt cggcgtcgaa gccgggggcg ccccagccgc tcgtcgtcgc ctcttcgagc
     4561 ttcgcggtcg tccacgcgga gttgaactcc gtgtactcca cacgcttggc gttcagcgtg
     4621 atgccggcct tgcgcatctg gtccgtcagg acctgcgggc ggtcggcgtc gtactgaccg
     4681 tacgtgtagt acgtcgcgtt gatcgtgaag tccttgaggc cagcagcgtc gaggagctgc
     4741 ttcgcctgcg ccgggtcgcc ggtcgccttc acccacggcc cgatctcgcc ggccttgtag
     4801 ctcggcatct tgttgaacag gaaggtgaac ggctggtcgg gcgcggacac gccgtagccc
     4861 tcgaacacca gctggatcgt ggtgtcgtga tcctgcgaga gcgagatcgc ccgccgcaca
     4921 cgctcgtcct tgaacttcgg gttcgagaca ttcatgccga agccgtagcc gccggccgtc
     4981 gggttgacca tgaacacctg cacgttgggg ttcgtgccca cgatgttctt gacgtcgctc
     5041 agcttcccgg cgagcgcgta ggcgtactcc acctgcccgg cgcggaaggc cgccgtgcgc
     5101 gccgtgccgt ccggcatgat cttgtagacc atgccatcga ggtgcagctt g
//
LOCUS       DAORPG010000057         5077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1047_length_5077_cov_3.642971, whole genome shotgun sequence.
ACCESSION   DAORPG010000057 DAORPG010000000
VERSION     DAORPG010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1047_length_5077_cov_3.642971"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PLX85_02155"
     CDS             complement(<1..291)
                     /locus_tag="PLX85_02155"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842484.1"
                     /note="regulates pyrimidine biosynthesis by binding to the
                     mRNA of the pyr genes, also has been shown to have uracil
                     phosphoribosyltransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase"
                     /protein_id="HRC62010.1"
                     /translation="MPELSERTHGIVLDEEGVRRTLTRLAHEVVERNGGLRDLVLVGI
                     QTRGVTLAERIAGIIESFEGERPPVGALDITLHRDDLAARGLDARTRPTALPN"
     gene            complement(481..1386)
                     /locus_tag="PLX85_02160"
     CDS             complement(481..1386)
                     /locus_tag="PLX85_02160"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HRC62011.1"
                     /translation="MSDLRIESHPELRRPILIAAWSGWNDAGESATGAIRFIRRRWRE
                     TPFASIDCDPFYDFTQNRPKVRLENGERVLDWPANEFVGHRREGDGPDVILFQGAEPH
                     LSWRAYSDALLELCKTFDVSAIVTLGALLAEVSHARPVHVQGSSEDPELRRMLGLPGP
                     TVSRYEGPTGIVGVFNNLAKEAGLPTASMWASVPHYVNASPNPKGTLALLEQLNRNLR
                     LDLRLHDLEVFVARFDAQVAEEIAKNPEMVDYAKRIEEHQDAEEAELLGQAPEPDDSS
                     EELPDAQSMVDELERFLREQRGGAS"
     gene            complement(1449..1700)
                     /locus_tag="PLX85_02165"
     CDS             complement(1449..1700)
                     /locus_tag="PLX85_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62012.1"
                     /translation="MADHDHQMTEVPPELLRSIGTAVGRVLGHWPGGTNWDVLGCVEC
                     GFAQVRFHNGAKLAARVQVRGWNLFLDPPAPATPAGTTE"
     gene            complement(1896..2351)
                     /locus_tag="PLX85_02170"
     CDS             complement(1896..2351)
                     /locus_tag="PLX85_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62013.1"
                     /translation="MDRRGPAGPFRVPVEPCTSPRRLTDMRVLLIAAAVLLLVACGDE
                     EGARVTSELAPIESVEVRVAESFPPQYFLDVTSGLPSGCASFDRYEVVRNGDTVEVSV
                     WNRIEIPKNGACTAIYGTAKHAITLGPDFQPGRTYTVQVNAVTKTFVGQ"
     gene            complement(2372..3394)
                     /gene="gap"
                     /locus_tag="PLX85_02175"
     CDS             complement(2372..3394)
                     /gene="gap"
                     /locus_tag="PLX85_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRC62014.1"
                     /translation="MANKLRVGINGLGRTGRQTVRAWWELHRDDWELVAINGRSDVAM
                     HTHLLRFDSDYGRFPAEIEDGPDSFSIGGREIKVFREDDPAQIDWNSVGADIVIESTG
                     EFESREMASKHLRGSVKKVIISAPGKDEDWTVIMGVNHLDYDPARHHVISNGSCTTNC
                     VVPMVKVLHDAFGVRRGFMTTIHAYTRDQNLVDAKHRDLRRARAAALNIIPTSTGAAR
                     SISKIIPELEGKMNGVAYRVPTPTVSVVDLVTDLAQPASKGAINEAFLTAAEGPMKGF
                     LYFEKQELVSTDFRGHPASAIFDSPSTMVLDNDMVKTMGWYDNEYGYACRITDLVAYL
                     GERGLS"
     gene            complement(3599..4510)
                     /locus_tag="PLX85_02180"
     CDS             complement(3599..4510)
                     /locus_tag="PLX85_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62015.1"
                     /translation="MSDGHAGHDEELPILDPPQRVSGMRVPDGWTLPGKPSGLSAAAE
                     DAWRQTGFLLAEDLRLIETDLDLQVRLVNAGYTPSARNMRMAGFASLWSRAFSCTSDA
                     VGLIRRGGYQSALPLLRQAVEHVAAQTQLGSELDEFRVWAHRAYARHPESRAEEVGLG
                     HYFGGEAIAGDDELRLIYRGASDLGRPNFGPTALFVANEASHARYPLNFADEAFHLGW
                     AQLLLGWALRTGVRQLHFGMHAREYFPAPDPLRERIVAHVRDAEAVLSDAGRCRLEEY
                     VDEDGRRRHLLVQFRRMPSDAAKRVLL"
     gene            complement(4507..>5077)
                     /locus_tag="PLX85_02185"
     CDS             complement(4507..>5077)
                     /locus_tag="PLX85_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRC62016.1"
                     /translation="ALDPATTELYREGQYHLEREGRVVDSAALTELWEEWANDYPIVS
                     IEDGMAEDDWDGWAGLRQRIGDRVQLVGDDLLVTNIERLARGIDERAANAILIKLNQI
                     GTLTETFEAIRMAHAAGWSTIISHRSGETEDTTIAHLAVAAGSGQIKTGAPARSDRNA
                     KYNELLRIEEQLGGGALYAGSTAFRKYGR"
BASE COUNT          765 a         1807 c         1645 g          860 t
ORIGIN      
        1 gttcgggagc gcggtcggcc gcgtgcgcgc gtcgagcccg cgcgccgcga ggtcgtcgcg
       61 gtgcagcgtg atatccagcg cgcccacggg cgggcgctca ccttcgaagc tctcgatgat
      121 gccggcgatg cgctcggcga gtgtgacgcc ccgcgtctgg atgcccacca gcacgaggtc
      181 gcggaggccc ccgttgcgct cgaccacctc gtgcgcgagc cgcgtcagtg tgcgtcgcac
      241 gccttcctca tcgagcacga tcccgtgcgt ccgctcggac agctcaggca tgaaaaaccc
      301 tccgaggaaa cctggagggt ggtgtcggaa atgcgcgcgt gaagtctcga tgcgagccga
      361 ggtgatgcat tctgcgtcct ttccggcctc acggggccgg tcttaaagac cgcgctgggg
      421 tcgcgtgaaa cagcctacca ccgaggcgtt tcctcacgca aagggtgtgt cggtgtgggc
      481 ttacgaagcc ccgccgcgct gctcccgcag gaagcgttcg agttcgtcga ccatcgactg
      541 tgcgtcgggc aactcttccg aggagtcatc cggctcaggc gcctggccga gcaactcggc
      601 ctcttcggcg tcctggtgct cctcgatccg cttggcgtag tcgaccatct cggggttctt
      661 cgcgatttcc tccgccacct gggcgtcgaa gcgcgcgacg aacacttcga ggtcgtgcag
      721 gcgcaggtcc agtcgcaggt tgcggttcag ctgttcgagg agcgcgagcg tccccttcgg
      781 gttcggcgac gcgttcacgt agtgcggcac gcttgcccac atcgaggcgg tcggcagtcc
      841 ggcctccttc gccaggttgt tgaagactcc gacgatgcct gtcgggccct cgtaccgcga
      901 caccgtcggc cccggcagtc cgagcatccg gcgcagctcc ggatcctccg aggagccctg
      961 cacgtgcacg ggccgcgcgt ggctgacctc cgcaagcagc gcccccagcg tgacaatggc
     1021 ggagacgtcg aacgtcttgc agagttcgag cagcgcatcg gagtacgcgc gccacgagag
     1081 gtgtggctcc gcgccctgga acaggatgac gtccggcccg tcgccttcgc ggcggtggcc
     1141 aacgaactcg ttggcgggcc agtcgaggac acgctcgcca ttctcgaggc ggactttcgg
     1201 gcggttctgg gtgaagtcgt agaacgggtc gcagtcgatg ctcgcgaacg gcgtctcgcg
     1261 ccagcggcgg cggatgaagc ggatcgcccc tgtcgccgat tcgcccgcgt cgttccaacc
     1321 agaccacgcc gcgatcaaga tcgggcgccg cagttctggg tggctctcaa tccgcaggtc
     1381 gctcatggct caagcatagt cccatcaccg aggaaacaca ccccgacacc cgcgcccctc
     1441 gacgccgctc attcggtcgt cccggccggc gtcgccggtg ccggtggatc gaggaacagg
     1501 ttccagccgc gcacctggac gcgggctgcg agcttcgcgc cgttgtggaa ccgcacctgc
     1561 gcaaacccgc actccacgca cccgaggacg tcccagttcg tcccgccggg ccagtgcccg
     1621 agcacgcgcc cgaccgctgt cccgatgctg cggaggagct ccggcggcac ctcggtcatc
     1681 tggtggtcgt ggtctgccat ggctcgctcc ttcgatcagg ttcctgaaca ttgaacgttc
     1741 gcgccggact tgccatcggc cggcgccggc ctcgcgtggc tcaactcacg aaccagctgt
     1801 ggccaggatt atgcgcttcg ccaataacgg ccgcgatccc gagaagtaat gacggaagcc
     1861 gcgccgtctc ctacctcgag ggccgctcgt gctggctact ggccgacgaa cgtcttcgtc
     1921 accgcgttca cctgcacggt gtacgtgcgc cctggctgga agtccggccc cagcgtgatc
     1981 gcgtgcttcg ccgtcccata gatcgcggtg caggcgccgt tcttcggtat ctcgatgcgg
     2041 ttccagacgc tcacctcgac ggtgtcgcca ttgcgcacaa cctcgtagcg gtcgaacgag
     2101 gcacacccgc tcggcaaccc cgacgtgaca tcgaggaagt actgcggcgg gaacgactcc
     2161 gcgacgcgca cctcgaccga ctcgatcggc gcaagttcac tcgtcacgcg cgcgccttcc
     2221 tcgtcaccgc acgcgacgag gagcagcacg gccgcggcga tgagcagcac ccgcatgtcg
     2281 gtgagacgcc ggggcgaggt gcagggttcc accgggacgc gaaaggggcc cgccggcccc
     2341 cttcggtcca tgcttcccat cggccgtatg gctacgacag cccgcgctcc cccaggtatg
     2401 cgaccaggtc ggtgatgcgg caggcgtacc cgtactcgtt gtcgtaccag cccatcgtct
     2461 tcaccatgtc gttgtccagc accatggtcg acggcgagtc gaagatcgcc gacgccgggt
     2521 gcccgcggaa gtccgtcgaa accagctcct gcttctcgaa gtagaggaat cccttcatcg
     2581 ggccctcggc cgccgtcagg aacgcttcgt tgatcgcgcc cttcgaggcc ggctgcgcca
     2641 ggtccgtcac caggtcgacg accgacaccg tgggcgtcgg cacgcggtac gccacgccgt
     2701 tcatcttccc ctcgagctcc gggatgatct tgctgatgga gcgcgccgcg cccgtcgagg
     2761 tcggaatgat gttcagcgcc gccgcccgtg cccgccgcag gtcccggtgc ttcgcgtcca
     2821 cgaggttctg gtcgcgggta taagcatgaa tggtcgtcat gaagccgcgc cggaccccaa
     2881 aggcgtcgtg caacaccttc accatcggca cgacacagtt cgtcgtgcac gagccgttcg
     2941 agatgacgtg atgccgggct gggtcgtagt cgaggtggtt cacgcccatg atgacggtcc
     3001 agtcctcgtc tttgccgggc gcggagatga tcaccttctt cacgctgcca cgcaggtgct
     3061 tcgaggccat ctcgcgcgac tcgaattcac ccgtcgactc gatgacgatg tccgcgccga
     3121 ccgagttcca gtcgatctgc gccggatcgt cctcccggaa caccttgatt tcgcgcccgc
     3181 cgatcgagaa gctgtccggc ccgtcctcga tctctgccgg gaagcgcccg tagtcgctgt
     3241 cgaaacgcag caggtgtgtg tgcatcgcga cgtcgctgcg accgttgatc gcgaccagtt
     3301 cccagtcgtc gcgatgcagc tcccaccagg cgcggaccgt ctgacggccg gtccggccga
     3361 gtccgttgat gccgactcgc agcttgttcg ccatcgtcct cgtgtctcct cgtgatactg
     3421 ccgcggggag tgcgggcggg tgctgactga cgcgcgtgct cacgcgaatt gtaacgctgc
     3481 cgtgtaccgc ccctgagcgc aagagaccca agtacggccg cccatgaaga caacatcgac
     3541 gacggatctc gggacgagtc tgggggccga tcggcacgcc ttggtgctgc gccggtccct
     3601 acaacagcac tcgtttcgcc gcgtccgaag gcatgcggcg gaactgcacc agtaagtgcc
     3661 gccgccggcc gtcctcgtct acatactcct cgaggcgaca gcgccccgcg tcgctgagca
     3721 ccgcctcggc gtcgcgcaca tgcgccacga tgcgctcccg gagcgggtcg ggcgccggga
     3781 agtactcgcg ggcgtgcatc ccgaagtgca gttgcctcac gccggtgcgc agcgcccagc
     3841 cgagcagcag ctgcgcccac ccgaggtgga acgcctcgtc ggcgaagttc agcgggtagc
     3901 gcgcgtggga cgcctcgttt gccacgaaca gcgccgtcgg cccgaagttc ggccgcccga
     3961 ggtcggacgc gccgcggtag atcaggcgca actcgtcgtc gcccgcaatc gcctcaccgc
     4021 cgaagtagtg cccaaggccc acctcctcgg cacggctctc cgggtggcgg gcgtacgctc
     4081 ggtgcgccca tacgcggaac tcatcgagct ccgagccgag ctgcgtctgc gccgccacgt
     4141 gctcgactgc ctgccgcagc agcggcagtg cgctctggta cccgccgcgc cggatcaacc
     4201 caaccgcgtc cgaggtgcac gagaacgccc ggctccacag cgaggcgaac cccgccatac
     4261 gcatgttccg cgcgctcggc gtgtatccgg cgttcacgag gcgtacctgc agatcgaggt
     4321 ccgtctcaat cagccgcagg tcctccgcaa gcaagaagcc ggtctgccgc caggcgtcct
     4381 cggcggccgc gctgaggccc gatggtttgc caggcagcgt ccagccgtcg ggcacacgca
     4441 tgcccgacac tcgctgcggc gggtcgagga tcggcagctc ctcgtcgtgg cccgcgtggc
     4501 cgtcgctcat cggccgtact tgcgaaaggc cgttgaccct gcgtacagcg cgccgccacc
     4561 gagctgctcc tcgatgcgca gcagctcgtt gtacttcgcg ttacggtcgg agcgcgccgg
     4621 cgcgcccgtc ttgatctgcc cggacccggc ggccaccgcg aggtgcgcga tggtcgtgtc
     4681 ctcggtctcc ccggaccggt gcgagatgat cgtcgaccag ccggcagcgt gcgccatgcg
     4741 gatcgcctcg aaggtctcag tgagcgtgcc gatctggttg agcttgatca ggatcgcatt
     4801 cgcggcccgc tcatcgatgc cgcgcgccag ccgctcaatg ttcgtgacga ggaggtcgtc
     4861 gcccacgagc tgcacccggt caccgatgcg ctggcgcagg ccggcccagc cgtcccaatc
     4921 gtcctccgcc atgccgtcct cgatcgacac gatcgggtag tcgttcgccc actcctccca
     4981 cagctcggtc agcgctgccg agtcgacgac acggccctcc cgttcgaggt ggtactggcc
     5041 ctcccggtac agctcggtgg tcgccgggtc gagcgcg
//
LOCUS       DAORPG010000058         5077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1048_length_5077_cov_2.968140, whole genome shotgun sequence.
ACCESSION   DAORPG010000058 DAORPG010000000
VERSION     DAORPG010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1048_length_5077_cov_2.968140"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PLX85_02190"
     CDS             <1..581
                     /locus_tag="PLX85_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC62017.1"
                     /translation="PIISMGWEHATNETFYEDVRIPAENVVGEVNRGWYVAMTLLDFE
                     RSNISGAITQRRNLTDLISYLKTDVGAERSQGRATLVRDKVADRWIESEVMFNFSFRI
                     ASMQASGVIPNYEASTSKIWNSELHQRSSNTGMKAFGLYANLWDRDDARTPLDARFTQ
                     DYVHSVVFSIFGGSNEIQRNIIATRGLGLPRS"
     gene            819..2042
                     /locus_tag="PLX85_02195"
     CDS             819..2042
                     /locus_tag="PLX85_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC62018.1"
                     /translation="MDWSDSEEQATFRKEVRDFINSKLPEYYKRRESRPAIGLEENWE
                     QDYVHGDAAAKQAATEWNAALTEKGWVAPHWPKEYGGAGMTTMEQFVFASEMAQAQAP
                     QPGGQGLSLLGPTLLVHGTEEQKAKYLAPTIRSEIRWAQGFSEPGAGSDLAGLQARAT
                     RDGDEWVINGQKMWTSTAHKTNWIFGLFRTDPDAPKHRGISFLIMDQTTPGISVRPII
                     SMGWEHATNETFYEDVRIPAENVVGEVNRGWYVGMTLLDFERSNISGAITQRRNLTDL
                     IKYLKTGDGVERSQGRANLVRDKIADRWIESEVMFNFSFRIASMQAAGVIPNYEASTS
                     KIWNSELHQRVCNTGMKAFGLYANVWDRDDARTTLDARFTQDYVHSVVFTIFAGSNEI
                     QRNIIATRGLGLPRS"
     gene            2503..3231
                     /gene="rplI"
                     /locus_tag="PLX85_02200"
     CDS             2503..3231
                     /gene="rplI"
                     /locus_tag="PLX85_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRC62019.1"
                     /translation="MGRHGRRPHYSWCPGSDTPRGSSVVKVLFLETVEGSGTRGEVKT
                     VANGYARNYLLPRGYAAPATPAILKRAAKLAAEEEQRQLSLDEQARRLVGKLDGQTLV
                     CAVRVGEQGRLYGSVTNADIAEKAGEILGEELDRRRVLLPEVIRQVGLYSVPLRLSRN
                     VTAEVQVAVVDVDAPEGVEAAVEALLAVPEEPEEMATGADDAASAADTAADGVSEQGQ
                     SSADSATSEAAGSAADQPEDSAAS"
     gene            3241..4662
                     /gene="dnaB"
                     /locus_tag="PLX85_02205"
     CDS             3241..4662
                     /gene="dnaB"
                     /locus_tag="PLX85_02205"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRC62020.1"
                     /translation="MVSTSRPAPLRPDSRPAAGLPPHDISAEEAVIAALLLDEDAYAR
                     VLPIVRPEDFFREQHAWAYEACQALADRGEAITIPTLAHELDRAGHLDALGGEPALVE
                     IAGRHFTAVGVEAHARIVARDALYRRLIQAAGQIAQLAYEGGPDATRVLAAAEGLLLG
                     IRNAEAAGDFTRLRDLLDQFLEDPGESADGLMVSAVRSGFMDLDSLLGGFKRGDLVIV
                     AARTGVGKSSFLLNLSRNVAVGQHGTVAFFALEMAGEQLAMRLLSTEADVDSTRLRLG
                     MHTEAEEARIMHAIGTLAETHIFIDDSAVLTVPEIRAKCRRLQSEYGLDLVIVDYLQL
                     LHGSGRADTRANEVGAISRGLKELARELGVPVLAAAQLSRAVETRTPHIPMLSDLRES
                     GSIEQDADIVMFIYREDMYTTREEWEEQHPGDPGGQHPTGIAQFIVAKHRNGPTGTVA
                     VRFVNRTASFQDLILRELPSYER"
     gene            4666..>5077
                     /locus_tag="PLX85_02210"
     CDS             4666..>5077
                     /locus_tag="PLX85_02210"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HRC62021.1"
                     /translation="MLPHHIQGFRRLADVVLDAFNELRGEGSTHALGTGLSELDSRIG
                     GFRRGDLVVVSGPTGAGKSSFVRSVVTDAILRSDAAIRFFSPEASAEILFLRAVSALA
                     GVDSSRIDLGDLRGTESSRVDEALGVLRQSRMRVH"
BASE COUNT          885 a         1899 c         1499 g          794 t
ORIGIN      
        1 gccccatcat ctcgatgggc tgggagcacg ccaccaacga gacgttctac gaggatgtcc
       61 gcatcccggc cgagaatgtc gtcggcgagg tcaaccgtgg ctggtacgtc gcgatgaccc
      121 tgctcgactt cgagcgttcg aacatcagcg gcgccatcac ccagcgccgc aacctcacgg
      181 acctcatcag ctacctcaag accgatgtcg gcgccgagcg ctcgcagggc cgcgccacgc
      241 tggtccgcga caaggtcgcc gaccgctgga tcgagtccga ggtgatgttc aacttctcgt
      301 tccgcatcgc ctcgatgcag gcctccggcg tgatcccgaa ctacgaggcg tccacctcaa
      361 agatctggaa ctccgagctg caccagcgct catcgaacac cggcatgaag gccttcggcc
      421 tctatgcgaa cctctgggac cgcgatgacg ctcgcacgcc gctcgacgcc cgcttcaccc
      481 aggactacgt gcacagcgtc gtcttctcca tcttcggcgg ctctaacgag atccagcgca
      541 acatcatcgc cacccgcggt ctcggcctgc cgaggagcta gccgcgttca gcgaccccat
      601 ggaccgaccc aagagcggtc cgaccgatcc cggtcgggcc gctctgcgtt tcactactcg
      661 gccccacgcg gccatcacga agcgccatcg atgccgtaaa atgcggctgc ctcgggggcg
      721 tcgccgcccg ccgatcgcat cgcagacgcc ggaaccgcgc ctcccgcccc ggcgacttcg
      781 cagctcgcag gagccgcacg gctcagaaag gaacttcgat ggactggtcc gacagcgaag
      841 agcaggcgac gttccgcaag gaagtccgcg acttcatcaa ctccaagctt cccgagtact
      901 acaagcgccg tgagagccgg cccgccatcg gtctcgagga gaactgggag caggactacg
      961 tccacggcga cgccgccgcc aagcaggccg cgaccgagtg gaacgccgcc ctcaccgaga
     1021 agggctgggt cgccccccac tggccgaagg aatacggcgg cgcgggcatg accaccatgg
     1081 agcagttcgt cttcgcctcc gagatggcgc aggcgcaggc gccgcagccc ggtggccagg
     1141 gtctctcgct cctcggcccc accctcctcg tgcacggcac cgaagagcaa aaggcgaagt
     1201 acctcgcccc caccatccgc agcgagatcc gctgggcaca gggcttctcc gagccgggtg
     1261 ccggctccga cctcgccggc ctccaggcgc gcgccacccg cgacggcgac gagtgggtca
     1321 tcaacggtca gaagatgtgg acctcgaccg cccacaagac caactggatc ttcggcctct
     1381 tccgcaccga cccggatgcc ccgaagcacc gcggcatcag cttcctgatc atggaccaga
     1441 cgactcccgg catctccgtc cgccccatca tctcgatggg ctgggagcac gccaccaacg
     1501 agaccttcta cgaggacgtg cgcatccccg ccgaaaacgt cgttggcgag gtcaaccgcg
     1561 gctggtacgt gggcatgacg ctcctcgact tcgaacgttc gaacatcagc ggcgcgatca
     1621 cccagcgccg caacctcacc gacctcatca agtacctgaa gacgggcgat ggcgtggagc
     1681 gctcgcaggg ccgcgccaac ctcgtgcgcg acaagatcgc ggaccgctgg attgagtccg
     1741 aggtcatgtt caacttctcg ttccgcatcg cctccatgca ggctgcgggc gtgatcccga
     1801 actacgaggc ctccacttcg aagatctgga actccgagct ccaccagcgc gtgtgcaaca
     1861 ccggcatgaa ggccttcggc ctctacgcca acgtctggga ccgcgacgac gcccgcacga
     1921 ccctggacgc ccgcttcacg caggactacg tccacagcgt cgtcttcacc atcttcgccg
     1981 gctccaacga gatccagcgc aacatcatcg ccacccgcgg cctcggcctc ccgaggtcct
     2041 agcgtcgccc cgagagcaga ccacgcagca ggcccggccg gatcattccg gccgggcctg
     2101 ctgcttacat tgaaccttga ctgcctgacg cgtcacccga cgcaggtccc ggaacggcga
     2161 tacttcccca accaacccgc gggggatgca cggggggcgg cctccccgcc aacgttgggg
     2221 caccaccaac cattcgaacg agcgacatcc gacgtcccac gacgagctct gtcgcccgga
     2281 tcgcctacgc tgccaatcga cagaacaaac gtttgacaac aggcgcacac aagttctacc
     2341 ctgctcctca caacagcaca tagcgcgagg aaccagccac cgccgacccc gattgcaccc
     2401 cacgacgcag cgcgggtaac ctgtgagtcc gtttcacaag actatccacg cctcgtccga
     2461 gtctcccacc gagaacttct ggacaacgtc gttggtaact gcatgggtcg gcacggccgg
     2521 cgcccgcact atagctggtg tcccggctcc gacacgccga gagggtcatc cgtcgtgaag
     2581 gtgctatttc tggagactgt cgagggttca ggcactcggg gcgaggtgaa aaccgtcgcc
     2641 aacgggtacg cccggaacta cctgttgcct cgcggctatg ccgcgccggc aacgccagcc
     2701 atcctcaagc gggctgcgaa gctcgccgcc gaggaagagc aacggcagct gtctctggac
     2761 gagcaggctc gcaggcttgt cggcaagctt gatggccaga cgctcgtgtg cgccgttcgc
     2821 gtcggagagc agggacgcct gtacggttcc gtcacaaatg ctgacatcgc cgagaaggct
     2881 ggggagattc ttggcgagga actggaccgg cgtcgcgtac tgctgcccga ggtgatccgt
     2941 caggtcgggc tgtactccgt accgcttcgg ctctcacgta atgtgaccgc cgaagtgcag
     3001 gtagccgtcg tggatgtcga tgcgccggaa ggcgtggagg cagccgtcga agctctgctg
     3061 gctgtgccag aggagcccga agagatggcc accggtgcgg atgatgcagc ctcggccgcc
     3121 gacacggccg ccgatggtgt gtcggagcag ggccaatcct ccgctgattc ggccacctcc
     3181 gaggctgctg gctccgccgc agaccagccg gaggattcgg ccgcctccta acccgccctg
     3241 atggtcagca ccagccggcc ggctccactc cggccggata gccgtcccgc cgctggcctc
     3301 ccgccgcacg acatctccgc cgaggaagcg gtcatcgccg cgctcctcct cgacgaggac
     3361 gcctacgccc gcgtcctccc catcgttcgg cccgaggact tcttccgcga gcagcacgcc
     3421 tgggcctacg aggcctgcca ggccctcgcc gaccgcggcg aggccatcac catccccacc
     3481 ctcgcccacg agctcgaccg cgccggccac ctcgatgccc tcggcggcga gcccgccctc
     3541 gtcgagatcg ccggccgtca cttcaccgcc gtcggcgtcg aggcccacgc acgcatcgtt
     3601 gcccgcgacg cgctctaccg tcgcctcatc caggccgccg gccagatcgc ccagcttgcc
     3661 tacgagggcg gccccgacgc cacccgcgtc ctcgccgccg ccgagggcct cctcctcggc
     3721 atccgcaacg ccgaggccgc cggggacttc acccgtctcc gcgacctcct cgaccagttc
     3781 ctcgaggatc cgggcgagtc cgccgacggc ctcatggtct ccgccgtccg ctccggcttc
     3841 atggatctcg attcgctcct cggcggcttc aagcgcggcg acctcgtcat cgttgctgcc
     3901 cgcaccggtg tcgggaagtc gtcgttcctg ctgaaccttt cgaggaacgt cgccgtcggc
     3961 cagcacggca ccgtggcgtt cttcgccctc gagatggccg gcgagcagct cgccatgcgc
     4021 ctgctctcca ccgaggccga tgtcgactcc actcgcctac gcctcggcat gcacaccgag
     4081 gccgaggagg cgcgcatcat gcacgccatt ggcaccctcg cggagactca catcttcatc
     4141 gacgactccg ccgtcctcac cgtccccgag atccgcgcca agtgccgccg tctccagtcc
     4201 gagtacggcc tcgatctcgt catcgtcgac tacttgcagc tgctccacgg cagcggccgc
     4261 gccgacacgc gcgctaacga ggtaggcgcc atctcccgag ggctcaagga gctcgcccgc
     4321 gagctcggcg tccccgtcct cgccgccgcc cagctctccc gcgccgtcga gacgcgcacc
     4381 ccgcacatcc cgatgctctc cgacctccgc gagtccggct ccatcgagca ggacgccgac
     4441 atcgtgatgt tcatctaccg cgaggacatg tacaccaccc gcgaggaatg ggaagagcag
     4501 caccccggcg acccgggcgg ccagcacccc accggcatcg cccagttcat cgtcgcaaag
     4561 caccgcaacg gccccaccgg taccgtcgcc gtccgcttcg tgaaccgcac cgcgtctttc
     4621 caggacctca tcctccgcga acttccgtcg tacgagaggt aagtcgtgct acctcaccac
     4681 atccaaggct tccgccgact tgccgacgtg gttctggacg cgttcaacga actacgaggt
     4741 gaaggatcaa ctcacgcact cggcaccggg ttatcggagc tggactcacg gatcggcggc
     4801 ttccgacgcg gagacctagt cgtcgtcagt ggtccgaccg gggcagggaa gtcgtcgttc
     4861 gtgcggtcag tcgtaaccga cgcgattctt cgatccgatg ctgccatcag gttcttcagc
     4921 ccggaggcat ccgccgagat tctcttcctg cgagctgtct cggctttggc cggtgtcgat
     4981 tcgtcgcgga tcgacttggg tgacctccgc gggacggagt cgagtcgcgt cgacgaagca
     5041 ctgggggtct tgcgccaatc gcggatgcgc gtgcacc
//
LOCUS       DAORPG010000059         5045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1062_length_5045_cov_3.815431, whole genome shotgun sequence.
ACCESSION   DAORPG010000059 DAORPG010000000
VERSION     DAORPG010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1062_length_5045_cov_3.815431"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PLX85_02215"
     CDS             <1..494
                     /locus_tag="PLX85_02215"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HRC62022.1"
                     /translation="TSSSAASAAAGAAAPTRGGGRLVVPRIGVDAPIVTLGLRADGTM
                     PAPSGPLEVASYAFAAPPGEDGNFVLAGHVDFANYGPAVFYRLRELRAGDTFLVRLDD
                     GTTFEYRVAWVAAYDEATAPVGEIVGPTTSATATLITCAGTFSAAAHAYDQRLVVRAD
                     LVV"
     gene            633..842
                     /locus_tag="PLX85_02220"
     CDS             633..842
                     /locus_tag="PLX85_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62023.1"
                     /translation="MPKLELPSFVSHGTSNAYTNYGCRCDPCRAAHSLYRSERRARDP
                     QKYTDYMRDYMRKYRARKKAAAEAS"
     gene            complement(936..1490)
                     /locus_tag="PLX85_02225"
     CDS             complement(936..1490)
                     /locus_tag="PLX85_02225"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRC62024.1"
                     /translation="MRIGLISDTHIPSSGEAPPDEVIEAFRGVDLILHAGHAYTAQCI
                     EWLERIAPVKSAESWLQGISESPMRTSTLQVFEFEGHRVGMTHELILKSLGDEVLPGA
                     IDRYYPKNESIAQALEVIFGQPVDIVVFGYTHQAMLEEHDGVLLVNPGSPNLVRQEIG
                     LGTVAILEVTPGARAAHIIDLATL"
     gene            complement(1531..1881)
                     /locus_tag="PLX85_02230"
     CDS             complement(1531..1881)
                     /locus_tag="PLX85_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRC62025.1"
                     /translation="MAILISFDIDGTLEFGDPPGGITLEMVKRAQDLGFLIGSCSDRF
                     PSAQKSLWEARDIQVDFVAAKHMLPDIRQRFEADIYVHIGDRDLDRQLAEQAGFQFLW
                     DHEGEAEPWLEWVK"
     gene            complement(1987..3318)
                     /locus_tag="PLX85_02235"
     CDS             complement(1987..3318)
                     /locus_tag="PLX85_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRC62026.1"
                     /translation="MASHPMTPNSWHRNREGLGIWHGRGKVAATGVGTSPTARRWDEK
                     PETSMGALTLIAIRKAIEDAGISPSEIDGIVITPESTTGVSNDPNTPPPEGWDQVFKM
                     VDYPQAGITRGDIDWILVNMPELTNVQFKMEGRGCMSVSFTTAAEAVARGLTHTCLVI
                     RGWHNLAGRYYVGQGAAAGDTLAGPQKYSGLWGAPACAGTALIFEEYCNKYGKSHDMM
                     APFIVEQRKNGLMNPDGFYSQHRPEMITVEDYLSGRWIAKPANIYDNDLPIQAALAVI
                     MTTAERAKNHRPKPAYVLTHAQTRPESQGVTPTLESWERLSDKMGRMLFEGAGIGPDD
                     LDFENMYDGFTLFHQFHLEGLRFGANGEKGRGYALDLYQEDISITGPNPVSPSGGNNG
                     NGRTRFWNHRDSILQVQGRSPSQISKPANIGVSGSHMPHMCDSLIWSSSPD"
     gene            complement(3366..3776)
                     /locus_tag="PLX85_02240"
     CDS             complement(3366..3776)
                     /locus_tag="PLX85_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRC62027.1"
                     /translation="MPKQSPIPDELDKPFYDACNEERLVVQYCPACSRYQFPPRNACG
                     GCLNPGIQWKQVSGRGRIQSRVVVYDTPVSVLIPDQPFNVATIALEEEPEIIMLSHLP
                     GTPVEQVPIGAQVEVIFETTPATGQKIPEWRVVS"
     gene            complement(4168..>5045)
                     /locus_tag="PLX85_02245"
     CDS             complement(4168..>5045)
                     /locus_tag="PLX85_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRC62028.1"
                     /translation="PMVMAQSAWDLQQFSKGRINIGLGSQVKGHNIRRFGGTWSPPAR
                     RMEEYVTMMRAIWRSWQFGERPEFVGEDYRYLLMTPAFNPGPIDYPFPKISIASVGPK
                     MAEVAGAVGDGLRPHGFMTEKYMRQTVLPALEKGARRAGRSLADIEVSVGGFSAFGET
                     QAEVEQAIDALRRPISFYGSTRTYHVVFQAHGLEALGMRLHELSLRGEWDKMLDAVTF
                     EHAAEMANACTYDDLPQYARQHFEYATRLRLPFGDDHAAGRYGGSSDAEGSAPSMSRE
                     RLDWLIKELHTVGAE"
BASE COUNT          768 a         1553 c         1838 g          886 t
ORIGIN      
        1 tgacctcgtc gagtgccgcg agtgcggccg cgggcgccgc ggcgcccacc agaggcggcg
       61 gacggctggt ggtacctcgg atcggggtgg atgcgccgat cgtgacgctg ggtctgcgtg
      121 ccgacggaac gatgcccgct ccttcggggc ccctggaggt ggcgtcgtat gccttcgcgg
      181 cgccgccggg cgaggacggg aacttcgtgc tcgcggggca tgtggacttc gcgaactacg
      241 ggccggcggt gttctaccgg ctgcgcgagc tgcgcgcagg cgatacgttc ctcgtgcggc
      301 tcgacgacgg cacgacgttc gagtatcgcg tggcgtgggt cgcggcctac gacgaagcga
      361 ccgcgccggt cggggagatc gtcggcccga caacttcggc gacggcgacg ctgatcacgt
      421 gcgctggcac gttcagcgcg gccgcgcatg cctacgacca gcgtctggtc gtgcgagcgg
      481 acctcgtcgt atgactcgac ccgttcgcac ggctgcgacg ccatgccgct caggcaccat
      541 gtcgatcgct gcggctccgc ctgtcgggca gcagtcgatg cttaaactca gcgcgcctcg
      601 aaccgaccgg tcatggttag agagaggtgg ctgtgcccaa gctcgaattg ccctcgttcg
      661 tttcacacgg cacgtcaaac gcgtacacga actacggctg tcgttgcgac ccgtgccggg
      721 cggcgcactc gctgtaccgg agtgagcggc gggcgcgcga ccctcagaaa tacacggact
      781 acatgcgcga ctacatgcgg aagtaccgcg cgcggaagaa ggcagctgcc gaggcctcgt
      841 gatgggcagg tggccggctc agcgacccgg cgggccgagg tgttcacctc gcccgccatg
      901 ggagctggct tgtcgccggt cttgaagacg cccgactaga gcgtggcgag gtcgatgatg
      961 tgagccgcgc gcgcgcccgg cgtcacctcg aggatggcca cggtaccgag gccgatctcc
     1021 tgacggacga ggttcgggct gccggggttc acgagcagca cgccgtcgtg ctcctcgagc
     1081 atcgcctggt gggtgtagcc gaagacgacg atgtcgaccg gctgtccaaa gattacctcg
     1141 agcgcctgcg cgatcgactc attcttcggg tagtagcggt cgatggcgcc ggggagcacc
     1201 tcgtcaccga ggctcttcag gatgagttcg tgggtcatac cgaccctgtg gccctcgaac
     1261 tcgaagacct ggagggtgct ggtacgcatt ggtgactcgc tgatgccctg gagccaggac
     1321 tcggctgact tcacgggcgc gatgcgctcg agccactcga tgcactgagc tgtgtaggcg
     1381 tgaccggcgt gcaggatgag atcgacgcct cgaaacgcct cgatgacctc gtcggggggc
     1441 gcctcgccgc tcgacgggat gtgggtgtca gagatcaggc cgatgcgcat gcgggccccc
     1501 gatggcgtta caagcttggg tgcgtcgagg ctacttgacc cattcgagcc agggctcggc
     1561 ctcaccctcg tggtcccaga ggaactggaa gccggcctgc tccgccagct ggcggtcgag
     1621 gtcgcggtcg ccgatgtgca cgtagatatc ggcctcgaag cgctgacgga tatcgggaag
     1681 catgtgcttg gcggcgacga agtcgacctg gatgtcgcgg gcctcccaga gtgacttctg
     1741 ggcggagggg aagcggtcgg agcagctgcc gatgaggaag ccgaggtcct gagcgcgctt
     1801 caccatctcg agggtgatcc cgccgggggg gtcgccgaac tcgagcgtgc cgtcgatgtc
     1861 gaagctgatg aggatagcca agctcatgtc cttcgcgaga gatgcgtagc tggtgatgcc
     1921 ggaagccggg gcctcctcgc gatggcgaga gggccccggc ccggattggt cgatcaggcg
     1981 tatcgcctag tcggggctgg agctccagat cagggagtca cacatgtgcg gcatgtgcga
     2041 gcccgagacg ccgatgttgg ccggcttcga gatctgggac ggcgaccgcc cctggacctg
     2101 caggatgctg tcgcggtggt tccagaatcg cgtgcgcccg ttgccgttgt tgccgccgct
     2161 cggcgaaacg gggttcgggc ccgtgatgct gatgtcctcc tggtagaggt cgagcgcgta
     2221 gccacggccc ttttcgccgt tggcaccgaa gcgcaggccc tccaggtgga actggtggaa
     2281 cagcgtgaag ccgtcgtaca tgttctcgaa gtcgaggtca tcgggaccga tgccagcgcc
     2341 ctcgaagagc atgcggccca tcttgtcgct gagccgctcc cacgactcga gggtgggcgt
     2401 gacgccctgg ctctcgggac gggtctgggc gtgggtcagg acgtaggccg gcttcggccg
     2461 gtggttcttg gcccgctcgg cggtggtcat gatgaccgcg agggcagcct gaatcgggag
     2521 gtcgttgtcg tagatgttgg ccggcttggc gatccagcgg ccggagaggt agtcctcgac
     2581 cgtgatcatc tcggggcggt gctggctgta gaacccgtcc gggttcatga ggccgttctt
     2641 gcgctgctcc acgatgaacg gggccatcat gtcgtggctc ttgccgtact tgttgcagta
     2701 ctcctcaaag atcaacgccg taccggcgca cgccggagcg ccccagaggc ccgagtactt
     2761 ctgcgggccg gcgagggtgt caccggcagc cgcgccctga ccgacgtagt agcgcccggc
     2821 gaggttgtgc cagccgcgga tgacgaggca ggtgtgcgtc agcccacgcg cgacggcctc
     2881 ggccgccgtg gtgaaggaga cggacatgca gccgcgaccc tccatcttga actgcacgtt
     2941 ggtcagttca ggcatgttga cgaggatcca gtcgatgtcg ccgcgggtga tgccggcctg
     3001 cgggtagtcg accatcttga agacctggtc ccagccttcc gggggcgggg tgttggggtc
     3061 gttgctgacg ccggtcgtgg actcaggcgt gatgacgatg ccgtcgatct cggaggggga
     3121 gatgcccgcg tcctcgatcg ccttgcggat ggcgatgagg gtgagggcgc ccatgctggt
     3181 ctcgggcttc tcgtcccagc ggcgggcggt gggcgaggtg ccgacacccg tcgcggcgac
     3241 cttgccgcgg ccgtgccaga taccgagacc ttcgcggttc cggtgccagc tgttgggggt
     3301 catcggatgt gatgccatgg gtcaggtgtc ctttcgcgtg tccctggcgg cgtgacgtcc
     3361 gccggctagc tgacgaccct ccactcgggg atcttctggc cggtggcggg ggtggtctcg
     3421 aagatcacct cgacctgcgc accgatcggg acctgttcaa ccggagtgcc gggaaggtgg
     3481 gagagcatga tgatctcggg ctcttcctcc agggcgatgg tggcgacgtt gaagggctgg
     3541 tccgggatga gcacggaaac cggggtgtcg tagacgacca cgcggctctg gatccggccg
     3601 cgcccgctca cctgcttcca ctggatgccg gggttgaggc agccaccgca ggcgtttcgc
     3661 ggcgggaact ggtaccggct gcaggcgggg cagtactgga cgacgaggcg ttcctcgttg
     3721 caggcgtcgt agaacggctt gtccagctcg tcaggaatcg gtgactgttt cggcaattcg
     3781 attctccaaa ctatcgtcga cgggtgagca ggatgtgatc tcccagtcag cctccttcca
     3841 accggacgtc gccatctcct gtgcactgga cgcggtgcga gcgggccgcg aagagcgggc
     3901 gcgctcgcca tcggagatgt tgtacacccg tcgagcctga aacggggcgg tcgtcgtgcg
     3961 tcgcttcgac gaaaacgtcc tcgcggggct cctgacgggt gcggcacctc cgttgctggc
     4021 tcgatcggca ggctcgccgg cgaccggcca gtcgcctcgc ggggagcctc ggcggaagca
     4081 gccgtccaat aacggcggta atcttacctc agagacggaa ggcgcggcag ccctcgcaag
     4141 ggccgaatgc tcctccgtct ccgggcgtca ttccgctccg accgtgtgga gctctttgat
     4201 cagccagtcg agtcgctcgc ggctcatcga cggggccgac ccttcggcgt ccgaggagcc
     4261 gccgtagcga ccggcggcgt gatcgtcgcc gaaggggagc cggaggcggg tggcgtactc
     4321 gaagtgctgg cgggcgtact gcggcagatc gtcgtaggtg caggcattgg ccatctcggc
     4381 agcgtgctcg aaggtgacgg cgtcgagcat cttgtcccac tcgcctcgca gcgagagttc
     4441 gtgcaggcgc atcccgagcg cctcgagccc gtgggcctgg aatacgacgt ggtaggtacg
     4501 ggtcgagccg tagaaggaga tcggacggcg gagggcgtcg atcgcctgct cgacctcggc
     4561 ctgggtctcg ccaaaggcgc tgaacccgcc gaccgagacc tcgatgtcgg caagggagcg
     4621 cccggcgcgc cgcgcacctt tctcgagggc gggcaggacg gtctgccgca tgtacttctc
     4681 ggtcatgaag ccgtggggcc ggagcccgtc gccgacggcg ccggcgacct cggccatctt
     4741 cgggccgacg ctggcgatcg atatcttcgg gaacgggtaa tcaatcgggc cggggttgaa
     4801 ggcgggggtc atgagcaggt agcggtagtc ctcgccaacg aactcggggc gctcgccgaa
     4861 ctgccaggag cgccagatgg cccgcatcat cgtgacgtac tcttccatgc ggcgcgcggg
     4921 gggcgaccag gtgccgccga agcgccggat gttgtggccc ttgacctgcg aaccgaggcc
     4981 gatgttgatg cggcccttgc tgaactgctg caggtcccac gcggactgcg ccatgaccat
     5041 cgggg
//
LOCUS       DAORPG010000060         5027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1074_length_5027_cov_3.311947, whole genome shotgun sequence.
ACCESSION   DAORPG010000060 DAORPG010000000
VERSION     DAORPG010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1074_length_5027_cov_3.311947"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /gene="coxB"
                     /locus_tag="PLX85_02250"
     CDS             complement(<1..927)
                     /gene="coxB"
                     /locus_tag="PLX85_02250"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRC62029.1"
                     /translation="MALLVACDAPQSTTEIAGNNNDKLWEPYRVVLVLAAIVFVLVLV
                     LTLGFSIWFRERPGRPARQIHGNTRLEVIWTLIPVAIVAMIAIPTFDALFKIDKDPPA
                     DAMKIEAIGHQWWFEFRYLDDKGETVLTTANELHLPEGKPVTIALRSVDVIHSFWVPA
                     ISGKVDMIPGHDNNISFTATKARPEPYLGQCAELCGLSHANMRFRVFVQAPADFDNWM
                     SDQKKDSTSATGDLASEGEQLFAQSACIGCHTVRGTSAQGKVGPDLTHVGSRTTVAAG
                     TLENNAQNLARWIRNSADVKPGSKMPPQGASAG"
     gene            1175..1738
                     /locus_tag="PLX85_02255"
     CDS             1175..1738
                     /locus_tag="PLX85_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRC62030.1"
                     /translation="MRMTSSPLGRTFRNQPVMLVAVAVVLIALAGITYAVATGVGEHT
                     PGQPAVGDAPPFSIAAFDGSSFDLAAHADGPVLLYFWASWCVPCRSEAPLIQRLWPEY
                     QAKGYTFVGMNIWDTDSDARKFARDFELTFPVVRDPENKVYLDYGVETLPMAFLIRPG
                     LQVERRYVGQLEEGDLRSMLDHLREPS"
     gene            1735..2172
                     /locus_tag="PLX85_02260"
     CDS             1735..2172
                     /locus_tag="PLX85_02260"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HRC62031.1"
                     /translation="MSQRLRTIFSSRRARLIAILVVATGAISVLAVSAAGSSMSYYVT
                     PDEFAKQIDVQDKRWRVGGRVVEGTIVEENGRPVSFVIQSEDGLQQMNISYDGIKPNL
                     FGPRAFVIVEGVADTPGHLIASSVIIKHESEFVTGTDQASTSK"
     gene            2169..4172
                     /locus_tag="PLX85_02265"
     CDS             2169..4172
                     /locus_tag="PLX85_02265"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HRC62032.1"
                     /translation="MNVLATVGAGALITALATSLFSCVAATVGLRARRPGLTVLARRA
                     WMTTAAFTSLAFGTLVIALLRDDFSLAYVASVSSREMETPMKWAALWSSQSGSLLYWT
                     WVMSILMAVFAWVTLPRIPWGAPHATALGSGVLAAFIAALVFLASPFEVSRITPDDGI
                     GLNPLLVDKGMLIHPPFLLGGLVSTSIPFVLGGAALLSARIDNAWLRSVRHWALLSWL
                     VLSIGNFLGGWWAYSVLGWGGYWGWDPVENSAMLPLLPMTAFLHSMMVQERRGMLKLW
                     NLALVAAAFGLAVFGTFNVRSGLVESVHSFAKSDVGSYFLVLLGIVVVLSIVLLVWRG
                     PYLKPDHDFDSLLSRESGLIVNNYLLTAIALIVLGGTLFPVFSELFDNSRITVGPPFY
                     NRVVGPLLIGLVVLAAIGTVLPWRQAAPGLLLRRFRTPVAVLVVSLVILGVLGMHDPF
                     ALAGTGSAIVLLYVTAREYFLGARGARRARGADPLSSFFGLFSRDRRRYGGYLVHIGF
                     GVMAIAIIGSNVYQEQVRTTVAPGGSFEAGGRTLTYEGLRERTGTSNGVETEIVAPVE
                     ISKDGKQVGYLEPGRRLFKNFPQQPMGIVAIDSSLRDDLYVFVQGWDSNGVAEFHVFV
                     NPLIIWLWIGGAIYVVGGLIAFAPVGVRAPEPVRLPSPAVQHS"
     gene            4193..4663
                     /locus_tag="PLX85_02270"
     CDS             4193..4663
                     /locus_tag="PLX85_02270"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HRC62033.1"
                     /translation="MLLRAVLVVVMVLSFGAALHGRAGAQTAADERALHIERQLLCPQ
                     CTNLRLDVCETQICIDMRAVIRERIDQGQSDGQIIDYFTGRYGDRVLADVPREGFNLW
                     LFAWVGGSLLLVAALGGYVLLRLRRTATPAPVRSDLSDDRWLDEQLAHDLDRSG"
     gene            4669..4950
                     /locus_tag="PLX85_02275"
     CDS             4669..4950
                     /locus_tag="PLX85_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62034.1"
                     /translation="MEWTVLIVLGVVAALLIALPAGRRAAGEARFDQEAGLLAERRQL
                     LAELHEVDEDAAAGRISADDRMAARRSLGPRLRAVTEALRSYGADEDRT"
BASE COUNT          768 a         1524 c         1783 g          952 t
ORIGIN      
        1 gccggccgaa gcgccctgcg ggggcatctt cgagcccggc ttcacgtccg cggagttgcg
       61 aatccagcgg gcgaggttct gcgcgttgtt ctcgagggtg ccggccgcga ccgtcgtgcg
      121 cgagccgaca tgcgtcagat cggggccgac cttgccctgc gcgctcgtgc ctcgcacggt
      181 gtggcacccg atgcatgcgc tctgtgcgaa gagctgctca ccctcggagg cgaggtcgcc
      241 cgtggcgctg gtcgagtcct tcttctggtc cgacatccag ttgtcgaagt ccgccggcgc
      301 ctgcacgaag acgcggaagc gcatgttggc gtgcgagagg ccgcagagct cggcgcactg
      361 accgagatag ggttccggcc tcgccttggt ggccgtgaac gagatgttgt tgtcgtggcc
      421 ggggatcatg tcgaccttgc cggagatggc gggcacccag aaggagtgga tgacgtcgac
      481 tgagcggagc gcgatggtga cgggcttgcc ctcggggagg tgcagctcgt tcgcggtggt
      541 gaggacggtt tcgcccttgt catcgaggta gcggaactcg aaccaccact ggtggccgat
      601 cgcctcgatc ttcatggcgt ccgccggggg atccttgtcg atcttgaaga gcgcatcgaa
      661 cgtcgggata gcgatcatgg cgacgatcgc gacggggatg agggtccaga tgacctcgag
      721 acgagtgttt ccgtggatct ggcgcgcggg ccgcccgggg cgctcgcgga accagatgct
      781 gaagccaagc gtgagcacga gtacgagcac gaacacgatg gccgcgagca ccaggacgac
      841 ccggtagggt tcccagagct tgtcgttgtt gttgccggcg atctccgtgg tgctctgcgg
      901 cgcgtcacag gcgacgagca gcgccaccag gagaacgagt gcgagaagcg gtaggagccg
      961 tctgccgcgg agagaggtca gaccgagtcg agtcgcgcgc aacaatgtcc cccttgctgg
     1021 cgtacggcac ggccccgcgt ggcgaatggg aaagcgggcc gcagtagcag taccatcggc
     1081 gcattatctg ggtcaagttg gaaacgggat agggcaacca cagagcgagg ttctgcgcac
     1141 gcgttgattg ggtccacccg agggtctagc ctcgatgcgc atgacttcct cgccgcttgg
     1201 acggacgttc aggaaccagc cggtgatgct ggtcgccgtg gcggtcgtgc tgatcgctct
     1261 cgctggcatc acgtacgcag tcgccacggg ggtgggcgag cacacgccgg gccaaccggc
     1321 ggtcggagac gcgcccccct tctcgatagc ggcgttcgac gggagttcct tcgacctcgc
     1381 ggcgcacgcc gatgggcccg tgctccttta cttctgggct tcgtggtgcg tcccctgccg
     1441 gagcgaggca ccgctcatcc agcggctgtg gccggagtac caggcgaagg ggtacacgtt
     1501 cgtcggcatg aacatctggg atacggacag cgacgctcga aagtttgcgc gggatttcga
     1561 gttaacattc ccagtggtgc gcgaccccga gaataaggtg tacctcgact acggcgtgga
     1621 gacgctgccg atggcgttcc tgattcggcc ggggctgcag gtggagcgcc ggtacgtggg
     1681 ccaactcgag gagggcgacc tgcgcagcat gctcgaccac ctgcgggagc catcgtgagc
     1741 caacgactgc ggacgatctt ctcgagccgg cgagcgcgcc tgatcgcgat cctcgtggtc
     1801 gcgaccgggg cgatcagcgt actcgcggtg tcggcggcgg gttcgtcgat gtcgtactac
     1861 gtgacgcctg acgagttcgc gaagcagatc gatgtgcagg acaagcggtg gcgagtcggc
     1921 ggccgggtcg tcgaggggac gatcgtcgag gagaacgggc gaccggtgtc cttcgtgatt
     1981 cagagcgaag atggcctcca acagatgaac atctcgtatg acgggatcaa gccaaaccta
     2041 tttgggccgc gtgcgttcgt gatcgtcgag ggggtggcgg acacccccgg ccatctgatc
     2101 gcctcatcgg tgatcatcaa acatgagtca gaattcgtca cgggcaccga ccaggcttcg
     2161 acatcgaaat gaacgtgctt gcgacggtcg gggccggcgc gctgatcacg gcgctcgcga
     2221 cctcgctgtt ctcctgcgtg gccgcgacgg tcggcctgcg tgcacgtcga cccggcctta
     2281 cggtgctcgc gcgtcgcgcg tggatgacca cggcggcgtt tacgtcactc gcgtttggga
     2341 cgctcgtgat cgcgctgctg agagacgact tctcccttgc ttatgtcgca tccgtctcct
     2401 cacgggagat ggagacgcca atgaagtggg cggccctgtg gagttcgcag tccgggtcac
     2461 tgctgtactg gacctgggtg atgtcgatcc tgatggcggt gttcgcgtgg gtgaccctgc
     2521 cgcgcattcc atggggtgca ccgcacgcga ccgcgctcgg gagcggcgtg ctggcggctt
     2581 tcattgcggc gctcgtgttc ctcgcatcgc catttgaggt gagccggatc acgcccgacg
     2641 acggcatcgg gctgaacccc ttgctcgtgg acaaagggat gctgatccac ccgccgttcc
     2701 tgcttggcgg cctggtcagt acgtcgatcc cgttcgtgct tggcggtgcg gctctcctgt
     2761 ccgcgcgcat cgacaacgca tggttgcggt cggtgcgcca ctgggcactg ctttcctggc
     2821 tcgtgctgag catcggcaac ttcctcggtg ggtggtgggc atactcggtg ctcggatggg
     2881 gcggctactg gggctgggac ccggtcgaga actcggcgat gttgccgctg ctgccaatga
     2941 cggcgttcct gcactcgatg atggtgcaag agcggcgggg gatgctgaag ctgtggaacc
     3001 tcgcgctcgt tgcggcagcc ttcgggctgg cggtattcgg caccttcaac gtacggagcg
     3061 gactcgtcga atccgtgcac tcgttcgcga agtcggacgt cgggtcgtac ttcctcgtgc
     3121 tgctgggcat cgtggtggtg ctgtcgatcg tgctgctggt gtggcggggg ccgtatctga
     3181 agcccgacca cgactttgat tcgctgctct cgagggagag cgggctgatc gtgaacaact
     3241 acctgctgac agcaatcgcg ctcatcgttc tgggcgggac gctgttcccg gtgttctcgg
     3301 agctattcga caactcgcgc atcacggtcg ggccgccctt ctacaaccgc gttgtcgggc
     3361 ccctgttgat cggactggtg gtgctcgcgg ctatcggcac cgtgctgccg tggcggcagg
     3421 cggctcccgg gctcctgctg cggcggttcc gaaccccggt ggccgtcctc gtcgtttccc
     3481 tcgtcatcct cggggtgctc gggatgcacg atccgttcgc actagccgga acgggttcgg
     3541 caatcgtgtt gctctacgtc acagctcgcg agtacttcct cggcgcacga ggggcgaggc
     3601 gcgcacgggg agcggaccct ctgtcgtcgt tcttcggtct gttctcacgc gaccggcgcc
     3661 gttacggcgg ttacctggtg cacatcggct tcggggtgat ggccatcgcg atcatcgggt
     3721 cgaacgtgta ccaggagcag gtgcgcacga cggtggcgcc cggcggatcg ttcgaggcgg
     3781 gtgggcggac gctcacatat gaggggctgc gcgagcgcac ggggacctcg aatggggtcg
     3841 agacagaaat cgtggcgccg gtcgagatca gcaaggacgg gaagcaggtc gggtacctcg
     3901 agccgggccg acggctgttc aagaacttcc ctcagcagcc gatggggatc gtcgcgatcg
     3961 acagctcgct gcgcgacgac ctgtacgtat tcgtgcaggg atgggacagc aacggggtgg
     4021 ctgagttcca cgtgtttgtg aatccgctca tcatctggct ttggatcggc ggcgccatct
     4081 acgtcgtcgg cggcttgatc gcgtttgctc cggttggggt ccgggcgccc gagccagtgc
     4141 gcctgccgag ccccgccgta cagcactcgt gactcgcatc gctcggcgtt tcatgctcct
     4201 tcgagccgtg ctcgtggtcg tgatggtgct ttcgttcggc gcggcgctcc acgggcgcgc
     4261 gggtgcgcag accgcggcag atgaacgcgc actacacatc gaacgccagt tgctgtgtcc
     4321 gcagtgcacg aacctgcggc tcgacgtgtg cgaaacacag atctgcatcg acatgcgcgc
     4381 cgtgatccgc gagcggatcg accagggcca gtccgacggc cagatcatcg actacttcac
     4441 gggccgatat ggcgaccggg tactcgcgga cgtacctcgc gaggggttca acctgtggct
     4501 gttcgcctgg gtcggcggat cgctgctgct ggtcgccgcc ctcggaggat atgtattgct
     4561 ccgcttgcgc cgcacggcga cgcctgcccc ggtgcggagt gacctatccg acgaccgttg
     4621 gctcgatgag cagctcgcgc acgacctcga tcggagcggg tagccgccgt ggaatggacc
     4681 gtgttgatcg tgctcggagt cgtcgcggcg ctgttgatcg cgctcccggc cgggcgacgc
     4741 gccgccggcg aggcgaggtt cgaccaagag gccggactgc tcgctgaacg acgacagctg
     4801 ctcgccgaac tgcacgaagt agacgaggac gccgcagccg ggcgcatctc ggcggacgac
     4861 cggatggccg cacgacgttc actgggacct cggttacgag cggtgacaga agcgctacgt
     4921 tcgtatggcg ccgatgagga ccggacatga ggtggaccgg agccgtggcg tgctccgcgc
     4981 ttctcctctt ggctaccgcg ttcgccagtc cggccctggt agagggg
//
LOCUS       DAORPG010000061         5023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1078_length_5023_cov_3.357488, whole genome shotgun sequence.
ACCESSION   DAORPG010000061 DAORPG010000000
VERSION     DAORPG010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1078_length_5023_cov_3.357488"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1103)
                     /locus_tag="PLX85_02280"
     CDS             complement(114..1103)
                     /locus_tag="PLX85_02280"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HRC62035.1"
                     /translation="MPVILLPPDVAVRLRPELGRIPELEVVTPPEEPGAFLAPDVLDR
                     VDAAFFNLGSGPSGTRRILGAARRATNLRWIHLGHSGVDDPVFEELIGRGVVVTNSAG
                     ANAEPIAQSALAGLLALNRGLPGWLDAQRRHAWEPDPDDLPSEQLPRELRGQTMVIFG
                     LGSIGRYVAQFARAFGIRVIGVRRTPATPEDGVDAWAPPARLDEVLPEADILVLTAPL
                     TSETRGTFDARRLDRLPAGAVVINVARGELVDEAALAERLASGRLGGAYLDVFVTEPL
                     PAESPLWELPNVLISPHDSARSAGTQGRVDAIFLEELGRWLRGEDSPRRARLD"
     gene            1255..2553
                     /locus_tag="PLX85_02285"
     CDS             1255..2553
                     /locus_tag="PLX85_02285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC62036.1"
                     /translation="MAVTVLRRLRSSSEHRFYGWNIVAAGAVNNFLTAGLAVWGFGVY
                     VSPLREEFGWSTAAIAAGFSIRSFQQGFLAPLAGIIVDRFGPRQVAVVGSLLMVAGLL
                     LFASTQSLWMYYLASVLMAMGQSASSFTAFSTAVMRWFARKRGRAMGTMHAGNGAGYF
                     LVPGVAFIVAVGGWREALVISAVLVLVIALPLALVLRNSPSDLGLAPDGDAPLPRADS
                     TATVNAAGLSGSSLGEAMHTSTFWLIALAQACGGASVQGWVVHTIPHLENVGFSLGAA
                     TMIGVGYAVCQLIFRPATGLIADRVGRKRIFVISFLLQSVGMLAFASLSSERFWLLPV
                     YYATFAAGQAAWVVLQAAIVADYFGPRRFATINGVVNTIQMPVGVISPIAAGYVFDHA
                     GSYAPVFIVYSGMSIVAAGSILLIRRQTWGARAETLPAAA"
     gene            complement(2557..3492)
                     /locus_tag="PLX85_02290"
     CDS             complement(2557..3492)
                     /locus_tag="PLX85_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="jacalin-like lectin"
                     /protein_id="HRC62037.1"
                     /translation="MDISVAGPSGGSGGEVFSDVSSVAPGVSLAAVELRAGDVVDAIR
                     SVYRHGTEEVPAPWHGGSGGELHRLELRAGEFITEIAGTYGEAPGHGHVVRSLSLQTN
                     LRPRRFRVGAGGGIPFRYEVGVDLELVGFAGASGAVLDAVGPLLRKRARRRGEALLLG
                     GPTGGEGGRAFSDAAFLGRSHGITVSRVTVRADRFLDAIAVDYVDADGALVGRRRHGG
                     DGGEEHQLTLRRGEYITAVSGRFGTLVDRIVIDTNRRKAVLAAGANFRPLEPRTPFGA
                     DYRYEAPPGYEIAGFLGRSATFVDALGVILRPRPA"
     gene            complement(3599..4054)
                     /locus_tag="PLX85_02295"
     CDS             complement(3599..4054)
                     /locus_tag="PLX85_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62038.1"
                     /translation="MSKLGLVAAAVVVAAIGIGIGVTRSAEAAVSGPAFYVDGVLYRT
                     VGTPTDLSHTGAPDSSYDVIYNLGGVQPYNVAEAAPGDRDFNGGRWQVHRVAFSNYAG
                     AIADAAVDLNGNGVLDSDTEVLSAIAHGYAGDLGVVKEFVCTVVRIPGH"
     gene            complement(4286..>5023)
                     /locus_tag="PLX85_02300"
     CDS             complement(4286..>5023)
                     /locus_tag="PLX85_02300"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HRC62039.1"
                     /translation="LSGRTAIEGEGALLDQRALGGRQGRLALAYLTLERRPVPREELA
                     AVLWPGEIPESWDAALSAVVSKLRAALRKVGLAASGIRSELGCYELVLPISTWVDVNE
                     AIDSLHAAEAADRSGSWRDIWGTAGVAYQITQRVFLPGEEGPWVERQRRLLGSIHLRA
                     TEFMAQVSLRNGEPAIAIALAEELLAVEPFRETGYQILMRAHMQLGNRAEALQAYERC
                     KRLLAEELGADPSPETQSVHLELLTTR"
BASE COUNT          752 a         1838 c         1661 g          772 t
ORIGIN      
        1 ggcctttcgg tcaccgcgtt cgccgtcgcc ctgctcgtca ccgtccccgc cctgcgcgag
       61 ctggcccgcc cctcggcggt cccgagccgc gcgtaaggcc tcgggcaggc gactcagtcg
      121 aggcgggctc gccgagggga gtcttcgccg cgcagccagc gccccaactc ttcgaggaag
      181 atggcatcca cacgaccctg cgtgccggct gaccgcgccg agtcgtgtgg actgatcagg
      241 acgttcggca gctcccagag cggcgattcc gccggaagcg gctccgtgac gaagacgtcg
      301 aggtacgcgc cgcccaaccg cccggacgcg aggcgctccg ccagggcggc ttcgtccacc
      361 agctcgccgc gcgcgacgtt gatcaccacg gcgcccgcgg gtaggcggtc gagccgcctc
      421 gcgtcgaacg taccgcgcgt ctcggacgtg agcggcgccg tcagcacgag gatgtccgct
      481 tccggcagca cttcgtcgag tcgcgcgggc ggcgcccagg cgtcgactcc gtcctcgggc
      541 gtggcggggg tgcggcgcac gccgatcacc cggatgccga atgcccgcgc gaactgcgcg
      601 acgtagcgac cgatgctccc gaggccaaag atgaccatcg tctgaccgcg cagctcgcgg
      661 ggcagctgtt ccgacgggag gtcgtcgggg tcaggctccc aggcgtgtcg ccgctgggcg
      721 tccaaccacc ccggcaggcc acggttcaac gccagcagcc cggcgagagc gctctgggcg
      781 atcggctccg cgttcgcgcc cgccgagttc gtgaccacga cgccccggcc tatgagctcc
      841 tcgaagaccg ggtcgtcgac tcccgagtgc ccgaggtgga tccagcgcag gttcgtcgcc
      901 cgtcgcgcgg cgccgaggat ccgccgcgtg cccgagggac cggacccgag gttgaagaac
      961 gcagcgtcga cacggtcgag cacgtccggc gcaaggaagg cgcctggttc ctccggcggg
     1021 gtgacaacct cgagctccgg aatgcgcccc agctcgggac gcagccgcac ggcgacgtct
     1081 ggcggcagca ggatgacagg cactcgagga gtctagcgcc ctcggaccgt cctgcgtcac
     1141 gacctcgcgc gggcctatga tttcgcaccg agggaacgtt ccgttagact aaaccaaacg
     1201 taccgcggcg cattccggcg gcgcactgct gaactgctga aaaggacgcc gcccgtggct
     1261 gtgaccgtgc tgcgcagact gcgctcgagt tccgagcacc gcttctacgg ctggaacatc
     1321 gtcgcggccg gtgcggtcaa caacttcctc acggccggcc tcgcggtctg gggcttcggc
     1381 gtgtacgtga gccccctgcg cgaggagttc ggctggagca ctgccgcgat cgcggccgga
     1441 ttctcgatcc gtagcttcca gcagggcttc ctcgctcccc tcgccggcat catcgttgac
     1501 cgcttcgggc cgcggcaggt cgcggtcgtc ggatccctac tgatggtcgc cggactgctg
     1561 ttgttcgcaa gcacgcagtc gctctggatg tactacctcg cgagcgtgct catggccatg
     1621 ggccagagcg caagctcgtt caccgcgttc tcgacggccg tcatgcgctg gttcgcccgc
     1681 aagcgaggcc gcgcgatggg cacgatgcac gcgggcaacg gcgccggcta cttcctcgtc
     1741 cccggagtcg cgttcatcgt ggccgtgggt ggctggcgtg aggcgctcgt catcagcgcc
     1801 gtgctcgtgc tggtgatcgc gcttcccctc gcgctggtgc tgcgcaacag cccgtcggac
     1861 ctcggactcg cccccgacgg cgacgccccc ctgccgcgcg ccgactccac ggcgacggtg
     1921 aacgccgccg ggctcagcgg ctcctcgctg ggcgaggcca tgcacacctc gacgttctgg
     1981 ctgatcgccc tcgcgcaggc ctgcggcggc gcaagcgtcc agggctgggt ggtgcacacc
     2041 atcccgcacc tcgagaacgt cggcttctcc ctcggggccg cgacgatgat cggcgtgggg
     2101 tacgcggtat gccagctgat cttccgcccg gcgaccggcc tgatcgcgga ccgggtcggc
     2161 cgtaagcgta tcttcgtcat ctccttcctg ctgcagtcgg tgggcatgct cgccttcgcc
     2221 tcgctgtcca gcgagcgctt ctggctgttg cccgtctact acgcaacctt cgccgcgggc
     2281 caggcagcct gggtcgtgct ccaggccgcc atcgtcgccg actacttcgg accgaggcga
     2341 ttcgccacca tcaacggcgt cgtgaacacg attcagatgc ccgtgggggt gatctcaccg
     2401 atcgcggccg gctacgtgtt cgaccatgcg ggctcgtacg ccccggtgtt catcgtctac
     2461 tccggcatga gcatcgtcgc ggcaggcagc atcctcctca tccgtcgcca gacctggggc
     2521 gcgcgcgccg aaacgctgcc ggccgccgcc tgaccgctac gccggccggg gtcggaggat
     2581 cacgccgagc gcatcgacga acgtggcgga gcgaccgagg aaaccggcga tctcgtaccc
     2641 cggcggcgcc tcgtaacgat agtcggcgcc aaacggcgta cgcggctcga gcggccgaaa
     2701 gttcgctccg gccgcgagga ctgcctttcg gcggttggta tcgatcacga tacggtcgac
     2761 cagcgtcccg aatcgccccg ataccgccgt gatgtactcg ccacgacgga gcgtgagctg
     2821 gtgttcctcg cccccgtcgc ccccgtgacg ccgtcgtccc acgagggcgc cgtcggcgtc
     2881 cacgtagtcc accgcgatcg catcgaggaa ccggtcggcg cgtacggtga cgcgagaaac
     2941 cgtgatgccg tgggaccgcc cgaggaacgc tgcgtcactg aaggcgcgtc cgccctcgcc
     3001 gcccgtcggg ccgccgagca gcagcgcctc acctcgtcgc cgcgcacgct ttcgcagcaa
     3061 cggcccgacc gcgtcgagga ccgcgccgct cgccccggcg aaacccacaa gctcgaggtc
     3121 gacgccgacc tcgtagcgga acgggatacc cccgcccgcg cccacccgaa accgtcgcgg
     3181 tcgcaggttc gtctgcaggc tcagcgaccg cacgacgtgc ccgtggcctg gcgcctcgcc
     3241 gtaggtgccc gcgatctccg tgatgaactc gcccgcgcgt aactcaagcc ggtgcagctc
     3301 gccgccggat ccgccatgcc atggcgccgg aacctcctca gtcccgtggc gatagacaga
     3361 gcggatcgca tcgacaacgt cgccagcacg cagctcgacc gccgcaagcg acacgccggg
     3421 cgccacgctc gagacgtccg agaagacctc accccccgac ccaccggacg gccccgcgac
     3481 tgagatgtcc attgcgcccc tcccgacgat gcgtgccagc gaaacagtga agcgccgcga
     3541 gcatagcgcg aggggacgct gacggatcga tccgcccgcg gccccctcac gtccggtgtc
     3601 agtgcccggg aatgcgtacg accgtgcaga cgaactcctt gaccacgccg aggtcgcccg
     3661 cgtagccgtg cgcaatcgcc gacagcactt cggtgtccga gtcgagcacg ccgttgccgt
     3721 tcaggtcgac cgccgcgtca gcgatcgctc ccgcgtagtt cgagaacgcc acgcggtgga
     3781 cctgccagcg accgccgttg aagtcgcggt cgccgggcgc cgcctcggca acgttgtacg
     3841 gctgcacccc gccgaggttg tagatcacgt cgtacgagct gtccggagct cccgtgtgcg
     3901 agaggtcagt gggcgtcccg accgtgcgat acagcacgcc gtcaacatag aaggccgggc
     3961 cgctcacggc cgcctccgct gacctcgtga ctccgatccc gatcccaatt gcggcgacga
     4021 ccacggcggc cgccaccagt ccaagcttgc tcatctgtct cctcctccgg aaccgacgaa
     4081 tgcgggtcgt ggccatcgac tgaggagcag cctagcgagg ggttcttacg attcgatgac
     4141 tcgcgcggtg ggcgccagct ggggaatcgc acagcggttc acgctcaccg tcgggggggc
     4201 gaggcggtgg attcggcgaa ctccggaccc gctcatttgt cgtggccgga gcgagctcct
     4261 ggccggattc gagacacgac agcggtcacc tggtcgtgag caactcgaga tgcacggact
     4321 gtgtctcggg ggatggatcg gcgcccagtt cctcggccag gagccgcttg cagcgctcgt
     4381 aggcctggag cgcctcggct cggtttccca gctgcatgtg cgctcgcatg aggatctggt
     4441 atcccgtttc gcggaacggc tctaccgcga gcaactcctc ggcgagtgcg atcgcgattg
     4501 cgggctcgcc gttgcgcagc gagacctgag ccatgaactc agtcgctcgc aaatggatcg
     4561 agcccaggag ccggcgctgc cgctcgaccc acggaccctc ctctcccggc aggaatacgc
     4621 gctgggtgat ctgatacgcg acgccggccg tcccccagat gtcgcgccat gaccccgagc
     4681 ggtctgcggc ctctgccgcg tgcagcgagt cgatcgcttc attcacatcg acccaggtcg
     4741 aaatgggcaa tacgagctcg tagcatccga gctcggagcg tatcccgctc gcagctaacc
     4801 ccacctttcg gagcgcggcg cgcaacttgc tcacgacggc gctgagcgcg gcgtcccacg
     4861 actccgggat ctctcccggc cagagcaccg cggccagctc ttccctcggt acgggccgcc
     4921 gttcgagcgt caggtaggcc agcgcaaggc ggccctggcg gccacccaac gcacgctggt
     4981 caaggagggc gccctcgccc tcgatcgccg tgcgtccgga cag
//
LOCUS       DAORPG010000062         4947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1113_length_4947_cov_3.274530, whole genome shotgun sequence.
ACCESSION   DAORPG010000062 DAORPG010000000
VERSION     DAORPG010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1113_length_4947_cov_3.274530"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLX85_02305"
     CDS             complement(<1..191)
                     /locus_tag="PLX85_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRC62040.1"
                     /translation="MAGGALEGIVVLDLTQGVAGPYATKYFSDYGAEVIKVERPGEGD
                     PARRLGPFPGDEPHLERSG"
     gene            349..747
                     /locus_tag="PLX85_02310"
     CDS             349..747
                     /locus_tag="PLX85_02310"
                     /inference="COORDINATES: protein motif:HMM:NF015862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HRC62041.1"
                     /translation="MPLTPDVAQRMIRASQDRARELGVAVSTAIVDADGRLFAFGRME
                     GAHWISVEVSQAKAFTGALLRRDGPDLQQMQPAVLSALSNLQGRAVMAMGSVTTLREN
                     DVVIAGIGCSGATDEQDGECAHAARDAYAG"
     gene            772..1719
                     /locus_tag="PLX85_02315"
                     /pseudo
     CDS             772..1719
                     /locus_tag="PLX85_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
     gene            1733..2560
                     /locus_tag="PLX85_02320"
     CDS             1733..2560
                     /locus_tag="PLX85_02320"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HRC62042.1"
                     /translation="MAESWDLLVSTDWLSEHLEGAGRDFVLIDAGEAVAYRRAHIPGA
                     VGVPHPYLKARDDRNHVMPPAEFEELAASLGVSNDTPVVIYDDNASLHAARVWWVFDY
                     YGHRDLRVVDGGFNAWLAEGRPLTSQVPRPAHGTFAARPQEAAVCLVDDLKAAVDGPA
                     APQLWDLRSDGEWTGEELRGNQRGGHVPGAKHLEWRGLMQGPPARRFRPREEIEAMLL
                     EAGIDPTAPTVTYCELGIRAAFGAFVLRLLGNGTARNYDGSMNEWANRPDTPLTLEV"
     gene            2673..3998
                     /locus_tag="PLX85_02325"
     CDS             2673..3998
                     /locus_tag="PLX85_02325"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC62043.1"
                     /translation="MEEQTIAPPARTSDAAEARGPRFYYGYWLIFAAAVGQFATVGSM
                     NYVPGVFLKPMTDEFGWNRAEFTLALTLGQFVLAFAGFFIGAYVDRHGGRRPMLVGAT
                     VLVLALLGHTQIHALWQWILLNGLLVTAGGAMAGNLVVNVTLSKWFVERRGRAIAIAS
                     MGVSFAGIIYPPATTAAVSAWGWRGAWAALAVASAVLLYPVAMLMRRAPEDYGLHPDG
                     RSSADVARGAGAVAQTEFATSLTRRQAIATPAFYLTVFAFGFGGLAIIVPVIQGIPFM
                     TDAGYSRGVASFMIVLTSIPSMLTKPFWGYLVDKAPANRLAAIGFVLNAVAILGIVLS
                     VNAHATAIVYASWLLLGFGWGGMIPLQEVVWATFFGRRYLGAVRSAGLPFSLLVSAGG
                     PLLVSFYFDVAGDYNGALLAVAVLGVVAAILVNLPRNPRRDMVVQPATA"
     gene            4132..>4947
                     /locus_tag="PLX85_02330"
     CDS             4132..>4947
                     /locus_tag="PLX85_02330"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC62044.1"
                     /translation="MAASRQAHHLNEQPLPGRHVVFALLSASMLAYIMQFGMVSVSIG
                     RLTTDLNAPLRWSGWVLTMFMIGQVIAMPVVGRISERVGAKNVFAIGFGLFGLASLAC
                     AVAPNVYLLIAARALQGAVGGGLQPAGTSLIGHVYGGGRMRAIGYYSSLMPFGAVLGP
                     VVGGLIVDHFGWRWTFGFNVPLGLAVAVVAFLMLPDAGRRPQSGRIDSLGMGLLALAV
                     TSLIFALTELGRRQGSPDPVLVGASLAISVAALAILVRHERRTASPIIDLDLLS"
BASE COUNT          696 a         1808 c         1643 g          800 t
ORIGIN      
        1 atcccgctgc gctcaaggtg cggctcgtca cccggaaacg ggcccagacg ccgcgcagga
       61 tcgccctcgc cgggccgctc caccttgatc acctcggcgc cgtagtcgga gaagtacttc
      121 gtcgcgtacg gcccggcgac cccctgcgtc aggtcgagca cgacgatgcc ctcgagtgct
      181 cccccggcca tgccgcctcc acatcgcgtc ccgcagtgtg cgcgacatca taacggctgt
      241 agtcccgcct cgcagcgccg ccgcggccac gcgacgaaga ccgcgccggg gcggcgaggt
      301 gatataactg ggccgccacc accccgaagc acgcccgagg agcctcgtat gcccctgacc
      361 cccgacgtcg cccagcggat gatccgcgcg agccaggaca gagcgcggga gctcggcgtc
      421 gccgtctcca cggccatcgt cgacgccgac ggccgcctgt tcgccttcgg gcgtatggaa
      481 ggcgcgcact ggatctccgt cgaggtgtcg caggcgaagg cgttcaccgg ggcgctgctg
      541 cgtcgggatg gtcccgacct gcagcagatg cagccggcgg tgctctcggc gctctcgaac
      601 ctgcagggcc gcgcggtcat ggcgatgggc agtgttacga ccctgcgcga gaacgatgtc
      661 gtgatcgccg gcatcggctg cagtggcgcc accgacgaac aggatggcga gtgcgcccac
      721 gcggcgcgtg acgcctacgc gggttagcac cggacgagga gacaggacga cagcatggcc
      781 ggacgagtcg gcatcctcat caatgcgttc aacgccgccg aggcggtgca gcgcatccgc
      841 gccgcggaag acctcggcat ccccaacgcc tggatgacga cgggcggcac cgcgccggac
      901 gcgctcacgg tgttcgccgg cgccgcgacc gtcaccgatc gcatcgtcat ggggtcctgc
      961 atcgtgccga cgtggctgcg ccaccccgtc gtgctggcgc agcagacgat cacgatcgaa
     1021 tcgctggcgc caggccgctt ccgcctcggc atcggcccga gtcacgagcc cgcgatgcgt
     1081 gccttcggcg tcgagtggcg caagccgctc accgagctga aggagtacct gatcacgctg
     1141 cgcgccctcc tccacgaggg ctccgtgagc ttcaaaggtg agcacgtgac catcgaggcg
     1201 cgcctggcac agccggtgca gaccccgctc ctcgcctcag cgctccgccc cggatcgttc
     1261 gaggcgtgcg gcgaactggc cgacggtgcc atctcctggc agtgcccggc cggctacctc
     1321 attgagcagg gcctgccggc gctccgtcgc ggagccgatg cggcaggccg cccggtcccg
     1381 ccgctgatcg cccacacccc catcgcgatc accgaggatc gcgaggcggt caaggcggcc
     1441 gccgcgcgcc agatctcgaa ctacagcaac atccccttct accgcgcgat gtatgagcag
     1501 gccggtgtcc cccaggagat gggcgaggtc ggtgacgacc tgatcgacgc gctcgtggtg
     1561 tgggggaacg aagaccgcgt ggtcgagcgc atcaagcaac tgctcgccga tggcatgggc
     1621 gaggtcctgg cgatgccgct cgtggtgggc ggcgaccgcg acgcctccct cgaccggtcc
     1681 ttccacgcgg tggcgcgcgc cgccgccgaa acagcctgac gaggagtccg cgatggccga
     1741 gagctgggac ctgctcgtct ccaccgactg gctcagcgag cacctcgagg gcgccgggcg
     1801 cgacttcgta ctcatcgacg ccggggaggc ggtggcatac cggcgggcgc acattccggg
     1861 cgcggtcggc gtcccgcacc cgtatctcaa agcgcgcgat gatcgcaacc acgtgatgcc
     1921 gcccgcggag ttcgaggagc tggcggcctc gctcggcgtc tcgaacgaca ccccggtcgt
     1981 catctacgac gacaacgcct cgctgcatgc cgctcgcgtc tggtgggtat tcgactacta
     2041 cggtcatcgc gacctccgtg tcgtcgacgg aggattcaac gcctggctgg ccgagggtcg
     2101 tccgctcact tcgcaggtcc cgaggccggc gcacggcacg ttcgccgcca ggccgcagga
     2161 ggctgccgta tgcctcgtcg atgacctcaa ggcggccgta gacggccccg cggcaccgca
     2221 gttgtgggac ctgcgcagcg acggcgaatg gaccggcgag gagctgcgcg ggaaccagcg
     2281 cggcggccat gtgccgggcg cgaagcacct cgagtggcgc ggcctgatgc agggcccgcc
     2341 cgcacgtcgc ttccggccgc gcgaggaaat cgaggcgatg ctgctggaag ccggcatcga
     2401 cccgacggcg ccgaccgtca cctactgcga actcggtatc cgcgcggcct tcggcgcctt
     2461 cgtgctgcgg ctgctcggga atggcacggc ccgcaactac gatggctcga tgaacgagtg
     2521 ggcgaaccgc ccggacacac cgctcacgct cgaggtctga ccggccacac acaccgtcgc
     2581 gcgcctgagc gcgagccggg tcataattcg cgtcgcccga tcaaacagcg cacatcagcc
     2641 acaggggttc gtactcccgg cgaaggacgc acgtggaaga acagacgatc gccccgcccg
     2701 cacgcacctc ggacgccgcc gaggcgcgcg gcccgcggtt ctactacggc tactggctga
     2761 tcttcgcggc cgccgtcggc cagttcgcaa ccgtcggctc aatgaactac gtccccggcg
     2821 tcttcctcaa gccgatgacc gacgagttcg gctggaaccg ggccgagttc acgctcgccc
     2881 tcaccctcgg gcagttcgtc ctcgcgttcg ccggcttctt catcggcgcc tacgtggacc
     2941 gacacggtgg gcgccgtccg atgctggtcg gcgccacggt gctggtcctg gccctgctgg
     3001 gacacacgca aattcacgcg ctctggcagt ggatcttgct caacggtctg ctcgtgaccg
     3061 ccggtggcgc gatggccggc aacctcgtcg tcaacgtcac cctgtcgaag tggttcgtcg
     3121 aacggcgcgg gcgcgccatc gccatcgcct cgatgggcgt gtcctttgcc ggcatcatct
     3181 atccccccgc gaccaccgcg gccgtcagcg cctggggctg gcgaggcgcc tgggcggcgc
     3241 tcgccgtcgc ctcggcggtc ctgctctacc cggtcgcgat gctcatgcgc cgggcccccg
     3301 aggactacgg cctccacccg gacggccgca gttcagcaga cgtggcccgc ggcgccgggg
     3361 cggtcgcgca gacggagttc gcgacgtcgc tgacccggcg ccaggccatc gcgacgccgg
     3421 cgttctacct gaccgtcttc gcgttcggct tcggcgggct cgcgatcatc gtgccggtga
     3481 tccagggcat ccccttcatg accgacgccg gatacagccg cggcgtcgcc tccttcatga
     3541 tcgtgctcac ctcgatcccg tcgatgctca ccaagccctt ctggggctac ctcgtcgaca
     3601 aggcgccggc gaaccggctg gccgcgatcg ggttcgtact caacgccgtg gcgatcctcg
     3661 gcatcgtgct gagcgtgaac gcgcacgcga cggcgatcgt gtacgcctcc tggctgctgc
     3721 tcggcttcgg ctggggcggc atgatcccgc tgcaggaggt cgtctgggcg accttcttcg
     3781 gccgccgcta cctgggcgcc gtgcgcagcg ccgggctgcc gttctcgctg ctggtctcag
     3841 ccggcggccc gctcctggtg tccttctact tcgacgtcgc cggcgactac aacggcgcgc
     3901 tgctcgccgt cgccgtcctg ggcgtcgtgg cggcgatcct ggtcaacctg ccccgcaatc
     3961 cgaggcgcga catggtggta cagcccgcca cggcctgatc tccatacgac gaatgtggtc
     4021 atcggcggat ccgatctggt cgcctgcggt atcgttcagt taggctgaag tatcgttcgt
     4081 gcccgtcgag gtgccccgca tcgcgccctc cagcgcgtgg aaagcgatga catggccgcg
     4141 tcccgtcagg cgcatcacct caacgaacag ccgctccccg gccgccacgt cgtcttcgcc
     4201 ctcctctccg cctcgatgct ggcctacatc atgcagttcg gcatggtgtc cgtttccatc
     4261 gggcggctca ccaccgacct gaacgcaccc ctgcgctgga gcggctgggt gctgaccatg
     4321 ttcatgatcg gccaggtgat cgcgatgccg gtcgtgggcc gcatctcaga gcgcgtgggc
     4381 gccaagaacg tgttcgccat cggattcggg ctcttcgggc tcgcgtcgct cgcctgcgcg
     4441 gtcgccccca acgtctacct cctgatcgcg gcgagggcgt tgcagggagc cgtcggaggt
     4501 ggactgcagc cggccggcac cagcctcatc ggccacgttt acggcggcgg ccgcatgcgc
     4561 gcgatcggct attactcgtc attgatgcca ttcggcgccg tgctcggccc ggtcgtcggt
     4621 ggcctcatcg tcgaccattt cggctggcgt tggacgtttg gcttcaacgt cccgttgggc
     4681 ctcgccgtcg cggtcgttgc attcctgatg ctgcccgatg ccggtcgccg gccgcagagt
     4741 gggcgtatcg actcactcgg gatgggcctg ctcgctctgg ccgtgacctc cctcatcttc
     4801 gcgttgaccg aactcgggcg acgccagggg tcgcccgacc cggtcctcgt gggggcctct
     4861 ctcgcgatct ccgtggccgc cctcgcgatc ctggtccgcc acgagcgccg cacggcgtcg
     4921 cccatcatcg acctcgacct gctctcg
//
LOCUS       DAORPG010000063         4905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1129_length_4905_cov_3.604948, whole genome shotgun sequence.
ACCESSION   DAORPG010000063 DAORPG010000000
VERSION     DAORPG010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1129_length_4905_cov_3.604948"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PLX85_02335"
     CDS             <1..471
                     /locus_tag="PLX85_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRC62045.1"
                     /translation="TLEAAARDLAAAGAHVSEVAPPRLEEASAITEGYWARLQSTSFS
                     GWRPAFAPTRDAEAVDRLLFEWERFTRDLLGFMRDFDVLLSPVAARPAPPLEGWGSEQ
                     YVYTLPYSLTRQPAAVVPFGRSSEGLPIGVQLAARPWEDHVALAAAIALEEALG"
     gene            complement(521..1453)
                     /locus_tag="PLX85_02340"
     CDS             complement(521..1453)
                     /locus_tag="PLX85_02340"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRC62046.1"
                     /translation="MPEYENVIYETLDDGAIARIWLNRPEFRNAQSFPLLDDLNDAFL
                     TAEMDDKVKVVILAGKGTSFSSGHDMGTPGQRFEESRFGATEGTHLRWNREWERFFFY
                     QQRWRDLRKTTVAQVHGPCIAAGMMLAWVCDLIVCADDTIFAEVVGPRLNMCGVEWVS
                     HPWEFGTRRAKEMMLLGDFIDAQEAHRLGMVNRIFAADRLEEETLHYARRIARLPMAT
                     ALMIKETINQTQDIQGYTNALKASFSIHQMNHAHWAEAGPMRPAALPSAAGQIRPGIQ
                     GLGFEPLRPFDVTSPGYNDDLDRIPAARETPANA"
     gene            1614..2228
                     /locus_tag="PLX85_02345"
     CDS             1614..2228
                     /locus_tag="PLX85_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRC62047.1"
                     /translation="MRIGVVSDTHVPEAGPDLPPEAYEALAGSDLILHCGDLHSLVVL
                     DRLEHIAPVISARGNGDTLHPWHRRPGVPEDPRVSEVHLLELEGFQLGITHDLETVEG
                     RPEEYADDLIRRVFGQRVDIALCGHTHIPLGWGLSTGTTILNPGSPTMPYGYPRILGT
                     VGLLTLTPGRFEFQALDLYTGDFHIEIAGPGAAPLQKGPRPKHI"
     gene            2320..3351
                     /locus_tag="PLX85_02350"
     CDS             2320..3351
                     /locus_tag="PLX85_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRC62048.1"
                     /translation="MTDYEMYELGDWPLESGEVLPDARLAYKTYGNLAPDRDNVVLFP
                     TAYGQRHTDCEPFFQEGLALDPQRWFIVVPNMFGNGLSSSPSNTRPPHDRGRFPRMTI
                     DDMVRAQHRLLTEHLGIERLALVTGTSMGALQTFHWGAHHPEMVERILPTCGAARCSV
                     HNYVFLEGVKAALTCDPAFKDGDYEERPERGLRAMGRVWAGWALSQAFYRERRYLELG
                     FADLEDYLVRAWEEPRLDRDANDMLSMIWTWQHADIAASPRFNGDFAKALAAIEAEAI
                     VMPGSTDLYFPPEDNAYEVARMPHAELRPIPSIFGHGAGGRSGRSPEDEAFVDAALKE
                     LLARPGRLR"
     gene            complement(3389..3829)
                     /locus_tag="PLX85_02355"
     CDS             complement(3389..3829)
                     /locus_tag="PLX85_02355"
                     /EC_number="3.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF020622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A2"
                     /protein_id="HRC62049.1"
                     /translation="MARVRPMLLAAAVAVLVLTSSTLTHHTATAATQPGVTEATRPTA
                     TETHHCGTDTIQVPDRGAYFDFADACANHDVCYATGGSEFDRYLCDRAFLSDMTASCN
                     AMWPVSSIWDTAQLRSRRLCYGVAYTYYLGVRLGGSAYFNYIPI"
     gene            4088..>4905
                     /locus_tag="PLX85_02360"
     CDS             4088..>4905
                     /locus_tag="PLX85_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62050.1"
                     /translation="MGALPLDLTLLVLLAVAALALLIFLLTGHPLVNIGGEEIGIVER
                     RYLGTPLPEGRVVAFPGQIGIRARVLAPGLHVMIPYLYRVMKEPMVEVGEDEVGLVES
                     IDGEPLQPGHIFGRRVEGHDGFQDGEVFLRNGGQKGPQVDVLPPGKYRVNTYLFRIRV
                     EPALIIPPNEVGVVTARDGGHIEAGRLLAAHVDGHNSFQDGETFLAAGGQRGPQIDVM
                     LPGRYRVNSDLFDVNVAPATMVEASKVGMVTARDGVPLPAGEVVAASVSGHNDY"
BASE COUNT          744 a         1821 c         1533 g          807 t
ORIGIN      
        1 accctcgagg cggccgcacg cgacctcgcc gccgccggcg cgcacgtcag cgaggtcgcg
       61 ccgccccgcc tcgaggaagc ctccgccatc accgaggggt actgggcgcg cctccagtcc
      121 acgtcgttct ccggctggcg tcccgccttc gcgcccactc gcgacgccga ggccgtcgac
      181 cgcctgctct tcgagtggga gcgcttcact cgagacctgc tcggcttcat gcgcgatttc
      241 gacgtgttgc tgtcgccggt tgccgctagg ccggcgccgc ccctcgaggg ctggggcagc
      301 gagcagtacg tctacacgct cccctacagc ctcacgaggc agcccgcggc cgtggttccc
      361 ttcggccgct cttccgaggg cctccccatc ggagtccagc tcgcggcgcg cccgtgggag
      421 gatcacgtcg cactcgccgc cgcgatcgcg ttggaggaag ccctcggcta gaaaccggcg
      481 gagggcgggc gagtgcttcg cccgccctcg tcgctaccac ttacgcgttc gcgggcgtct
      541 cccgcgccgc cgggatgcgg tcgaggtcgt cgttgtaccc cggcgaggtg acgtcgaacg
      601 gccgcagggg ctcgaagccc aggccctgga tgccgggccg gatctggccg gccgccgacg
      661 gcagcgccgc gggccgcatc ggccccgcct ccgcccagtg cgcgtggttc atctggtgga
      721 tcgagaacga tgccttgagg gcgttcgtgt acccctggat gtcctgcgtc tggttgatcg
      781 tctccttgat catcagcgcc gttgccatcg gaagccgcgc gatccgccgc gcgtagtgca
      841 gcgtctcttc ctcgagccgg tcggccgcga agatgcggtt gaccatcccg aggcggtgcg
      901 cctcctgtgc gtcgatgaag tcaccgagca gcatcatctc cttcgcgcgc cgcgtcccga
      961 actcccacgg atgggacacc cactcgacgc cgcacatgtt cagccggggc cccacgacct
     1021 cggcgaagat cgtgtcgtcg gcgcacacga tcaggtcgca cacccacgcc agcatcatgc
     1081 ccgccgcgat gcacgggcca tgcacctgcg ccaccgtcgt cttgcgcagg tcccgccacc
     1141 gctgctggta gaagaagaag cgttcccact ctcgattcca gcgcaggtgc gtgccctcgg
     1201 tcgccccgaa gcgcgactcc tcgaagcgct gcccgggcgt ccccatgtcg tgcccggagg
     1261 agaacgaggt ccccttcccc gcgaggatga cgaccttcac cttgtcgtcc atctcggccg
     1321 tcaggaacgc gtcgttcagg tcgtccagga gcgggaagct ctgcgcgttg cggaactcgg
     1381 gccggttcag ccagatccgc gcgatggcgc cgtcatccag cgtctcgtaa atgacgttct
     1441 cgtactcagg catgcttggg tcccccttcg gacacctgcg gccgacggcc tcgtttgcgc
     1501 cgcgttgtac cgccggcccc aaggactgtc aacgagcgct gccgtccgat gtgccgcatc
     1561 tcgcttccac gggtacgatc gtgcctacgc ctcggaggag gcgaggaggt gacatgcgca
     1621 tcggcgtcgt ctcagacacc cacgtccccg aggcgggccc ggatctccca cccgaggcgt
     1681 acgaggcgct cgccggcagc gatctcatcc tgcactgcgg cgacctgcac agccttgtgg
     1741 tcctcgaccg cctcgagcac atcgcccccg tcatctccgc ccgcggcaac ggagacaccc
     1801 tccacccctg gcaccgccgc cccggtgtcc ccgaggaccc tcgcgtctcc gaggtgcacc
     1861 tcctcgagct cgagggcttc cagctcggca tcacccatga cctcgagacc gtggaaggcc
     1921 gccccgagga gtacgcggac gacctcatcc gccgcgtctt cgggcaacgc gtggacatcg
     1981 cgctctgcgg ccacacccac atcccgctcg gctggggcct ttcgaccggc acgacgatcc
     2041 tcaaccccgg cagcccgacg atgccctacg gctacccgcg catcctcggc acggtgggcc
     2101 tgctcacact cacccccggc cggttcgagt tccaggcgct cgacctctac acgggcgact
     2161 tccacatcga gatcgccggg ccgggggccg cgcccctcca gaagggcccg aggccaaagc
     2221 acatctgagg ctccccgcgc cgctcctgcc cgtgacgacg tctgtgttag attgcctcgg
     2281 gcccggcggc agctgccggc gtttcgaggg aggcagcgcg tgaccgatta cgagatgtac
     2341 gagctcggcg actggcccct cgagtccggc gaggtcctgc ccgacgctcg cctcgcctac
     2401 aagacctacg gcaacctcgc gccggaccgc gacaacgtcg tcctcttccc gaccgcctac
     2461 ggtcagcgtc acaccgattg cgaaccgttt ttccaggagg gtctcgccct cgacccgcag
     2521 cgctggttca tcgtcgtccc gaacatgttc ggtaacggcc tctcctcctc gccgagcaac
     2581 acccggccgc cccacgaccg cggccgcttc ccccgcatga ccatcgacga catggtgcgc
     2641 gcccagcatc gactcctcac cgagcacctc ggcatcgagc gcctcgcgct cgtgaccggc
     2701 acctcgatgg gcgcgctcca gacgttccac tggggtgccc accaccccga gatggtcgag
     2761 cgcatcctcc cgacgtgcgg cgccgcgcgc tgctccgtcc ataactacgt cttcctggag
     2821 ggtgtgaagg ccgcgctgac ctgcgacccc gccttcaagg acggcgacta cgaggagcgc
     2881 ccggagcgcg ggctgcgcgc gatgggccgc gtctgggcgg gctgggcgct gagccaggcc
     2941 ttctatcgcg aacgccgcta cctggagctc ggcttcgccg atctcgagga ctacctcgtc
     3001 cgtgcctggg aggagcctcg cctcgaccgc gacgccaacg acatgctctc aatgatctgg
     3061 acctggcagc acgccgacat cgccgccagc ccccgcttca atggcgactt cgcgaaggcg
     3121 ctcgcagcca tcgaggcgga ggcgatcgtg atgcccggca gcacggacct ctacttcccg
     3181 cccgaagaca acgcctacga ggtcgcgcgc atgccgcacg cggagctgcg cccgatcccg
     3241 tccatcttcg gccacggcgc cgggggccgc agtgggcgca gcccggagga cgaggcgttc
     3301 gtcgacgccg ccctcaagga actcctcgca cgtcccggac gattgcgatg atccgcggat
     3361 cagtccgcgg actgcctgcc cgctctactc aaatcgggat gtagttgaag tacgcgctgc
     3421 cgccgaggcg cacacccagg taataggtgt acgcaactcc gtagcagaga cgccggctgc
     3481 gcaattgcgc tgtgtcccag atgctcgaca ccggccacat cgcgttacat gacgcggtca
     3541 tgtccgagag gaaggcacgg tcgcacaggt accggtcgaa ctcgctgccg cccgtggcgt
     3601 agcacacatc gtggttcgcg catgcgtcag cgaagtcgaa ataggcccct cgatccggga
     3661 cctggatcgt gtccgttccg cagtggtgag tctcggtggc ggtcggacga gtcgcctcag
     3721 tgacgccagg ctgcgtcgca gccgtcgccg tatgatgcgt cagcgtgctc gacgtcagga
     3781 caagcaccgc gacggccgcc gccagcaaca ttggtcgaac gcgagccatc tgtcaccgcc
     3841 tttacgttcc ctgaactcgc tcacccgccg gccagcgggc aggcccgtct acggcgtctc
     3901 ctgaggagcg tcgccgtttg cgccgcatcg tactcccggt tgtcgtcgct gaacacgtcc
     3961 gcgggtcatg tttcgtcgca gaatcaacgc gcgataacga tcggtgccgt acgccttaca
     4021 agagcgtaga ttcgtgatat cgagctgctc gatttgcgca ggctcgagac gtgcgaaagg
     4081 cgatgacatg ggcgcgctac cactcgatct cacgctgctg gtgctgttgg ccgtcgccgc
     4141 gctggcactc ctcatcttcc tcctcaccgg ccaccccttg gtcaacatcg gcggcgagga
     4201 gatcggcatc gtcgagcgcc gctacctcgg cacgcccctc cccgagggcc gagtcgtcgc
     4261 gttcccggga cagatcggca ttcgtgcccg cgtcctcgcg ccggggctgc acgtcatgat
     4321 cccgtatctc taccgcgtca tgaaggaacc gatggtcgag gtcggcgagg acgaggtagg
     4381 cctcgttgaa tccatcgacg gagagccgct ccagcccggc cacatcttcg gccggcgtgt
     4441 cgaaggtcac gatggattcc aggatggcga ggtgttcctc cgcaatggag gccagaaggg
     4501 cccccaggtc gacgtgctgc cgccgggcaa gtaccgcgtg aacacatacc tctttcgcat
     4561 ccgcgtcgag cccgcgctca tcattccgcc gaacgaggtg ggagtggtca ccgctcgcga
     4621 cgggggccat atcgaagccg gccggctgct tgcggcccac gtcgacggtc ataactcctt
     4681 ccaggatggc gagacgttcc tcgctgctgg cgggcagcgc ggcccacaga tcgacgtcat
     4741 gctgcccggc cgctatcgcg tgaactcgga cctcttcgac gtcaacgtcg cccccgcgac
     4801 catggtcgag gccagcaagg tcggcatggt caccgctcgc gacggtgtcc cgctgccggc
     4861 cggtgaggtc gtcgccgcca gcgtctccgg ccacaacgat tacca
//
LOCUS       DAORPG010000064         4830 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1163_length_4830_cov_3.528168, whole genome shotgun sequence.
ACCESSION   DAORPG010000064 DAORPG010000000
VERSION     DAORPG010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1163_length_4830_cov_3.528168"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..528
                     /locus_tag="PLX85_02365"
     CDS             88..528
                     /locus_tag="PLX85_02365"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRC62051.1"
                     /translation="MREIRTEVAIAAPLEAVWSVVSDFGAYAEWNPFLPAFEGRPVAN
                     TNGRLTAAIPGSKPRTTPVTILEADAPRRLAWRGQLLGSWLFSGTHFLELTPSDGGTR
                     VVNREEFRGLLAPLLLRQLGRGLPAGYEAMNAALKARVERAGRA"
     gene            complement(538..3045)
                     /locus_tag="PLX85_02370"
     CDS             complement(538..3045)
                     /locus_tag="PLX85_02370"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRC62052.1"
                     /translation="MTTMPLEGVRVIDLTRYTAGPFCTRILGDYGADVIKIEAPEGDP
                     GRQLPPFVKDEPGTERSGTFLFLNTNKRSVILDLTTAEGRERLLDLVRTAQVVVENFA
                     PGTMERLGLGYDILAEANPRIVLTSLSNFGQEGPYRDWLATDLTVYAMAGPMLATGNI
                     EHEPVRVAGRMATYQTAYMAALATVVAVQAAELRGRGEHVDVSMLEALTNNVDMRLTQ
                     LQAYQYSGRIGTRSGLASLVGSGVFPCADGYVQLTGGLQGRLILTMRMIGCEELMERP
                     EWQAPGAPTDPERIEEFLAYLLPWTLSHTKREIRDACELYGVLGAPLNTVADMLTDPN
                     FVAREFFQTIDHPSTGPAQYPGWHFRMHTDEGPMPPRRRAPLLGEHTDEVLAELSPRP
                     TTPATGEDRLGDRLPLEGLRVIDFTVVWAGPYATMHLADWGAEVIRIESRQHLAPNTR
                     GQLAHMTRAMVANNPAAASWYPDGEPGADPWNRNALFNGHGRGKKSMTIDLSRPEGQE
                     QFERLVAQADALIENNLPPNIEKQGITWERLSKINPRLVLVRVPAFGLEGPYRAYRTL
                     GNHMESIAGHPVIRAYPDLSLEYAPTGVPADAASGIGGATALLLGIRYRDRTGKGLMM
                     ELATAENMVGLLGEFVMDYTINGREWEHMGNDHWWLAPHNVYPCQGDDTWVTIAARND
                     EEWRALCGVMLRPDLASDPRFIDNANRHAHRRELDRIIGAWTVNADARWIMHRLQAAG
                     VPAGVVNTEPGILADPQHRARDFFQEIEHPSTGRQLHPRRAWRASRTPERPMRHPPRL
                     GEDNEYVYRELLGFSEERYQEWVASGHIGMAYEDSVP"
     gene            complement(3211..>4830)
                     /locus_tag="PLX85_02375"
     CDS             complement(3211..>4830)
                     /locus_tag="PLX85_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC62053.1"
                     /translation="TAAATGTAAATGPGRLVKDADMPYPYEFPDPAGTAKKGGSFVHG
                     SNFQFTPADPTVNLTGGTLIQFAPVYDRLLGYKMGPTADTTKVDVIPGLAESWERSPD
                     GLAITFKLRANAKWQNRPPLNGRPFVAEDVKYAYERMKASGAAASFFVGVKSIDAVDP
                     RTVKVSLDQPLVDWLTVTPPRREAPIFPKETVDAGTIQKTIVGTAAFIVDKQEDQRIT
                     FVKNPDYWGPAPNVDGGEVRVIVDLNARIAALRAGQLDHAGNIASSYRDTKSIADTIP
                     NVQVQMSRVTSGGHGFLMNPDNPKFKDERVRRALSLAVNRDEMIQIVYGGYGRVHGLI
                     PWLFAFEQEPKGDQLGKWMKYDPAQSKQLLDAAGVKDLAINEVHYDYLPTWKQDGELL
                     VDQYRKSGITLNLRTDEYTAYTSVLTGRKIEEATITGWGVSGTTPDPYVYDQLYSKSP
                     QNRWLINDPVIDDLSQKQRVELDPAKRRALIKQIYDRDMDMVYRFAFPGGNGFYLMQP
                     WVRNMRPFGALGSSSDVWDTGIQMKDLWIDK"
BASE COUNT          662 a         1614 c         1687 g          867 t
ORIGIN      
        1 gatggtggtc gcgatgggtc tcgtggcgct gacgctgccg accgtgcgca accttgacta
       61 gccgcgggcc ggtggagtcc cgccgtcatg cgtgagatcc gcaccgaagt ggccatcgcc
      121 gctcctctcg aggcagtctg gagcgtcgtc tccgatttcg gagcctacgc agaatggaat
      181 cccttcctgc cggcattcga gggccgtcca gtcgcgaaca cgaacgggcg gctcactgcc
      241 gccattcccg gcagcaagcc ccggacgact cccgtcacga tcctcgaggc cgacgcgccg
      301 cgacgcctcg cctggcgcgg gcagttgctc gggtcttggc tcttctcggg gacgcatttc
      361 ctcgagctca cgccgagcga cggcgggact cgcgtcgtca accgcgagga gttccgcggc
      421 ctgctcgcgc cgctgttgtt gcgccagctc ggccgcggcc tgcccgcggg ctacgaggcg
      481 atgaacgccg ccttgaaggc gcgtgtcgag cgcgccggcc gtgcctgacc cggctcgcta
      541 cggcacgctg tcctcgtagg ccatgccgat gtgccccgac gccacccact cctggtagcg
      601 ctcctcgctg aacccgagca actcgcggta gacgtactcg ttgtcctcgc cgagccgagg
      661 ggggtgtcgc atcgggcgct cgggcgtgcg gctggcccgc caggcgcgcc gcgggtgcag
      721 ttgacggccg gtcgatgggt gctcgatctc ctggaagaag tcacgcgcgc gatgctgggg
      781 atcggcgagg atgccgggct cggtgttcac cacgccggcc ggtacgccgg cggcctgcag
      841 gcggtgcatg atccaccgcg cgtccgcgtt gaccgtccag gcgccgatga tgcggtcgag
      901 ctcgcggcgg tgtgcgtggc ggttcgcgtt gtcgatgaag cgcgggtcgc tcgcgaggtc
      961 ggggcgcaac atgacgccgc acagggcgcg ccattcctcg tcattccgcg cggcgatcgt
     1021 cacccacgtg tcgtcgccct ggcacgggta cacgttgtgg ggggccagcc accagtggtc
     1081 gttgcccatg tgctcccatt cgcggccgtt gatcgtgtag tccatgacga actcgccgag
     1141 gagccccacc atgttctcgg ccgtcgccag ctccatcatc aggcccttgc cagtccgatc
     1201 ccggtagcgg atcccgagca gcagcgccgt cgcgccgccg atgccagacg cagcgtccgc
     1261 cggtacgccc gtgggcgcgt actcgaggga caggtcgggg tacgcccgaa tcacgggatg
     1321 cccggcgatc gactccatgt ggttgccgag cgtgcggtag gcgcggtacg gcccctcgag
     1381 accgaacgcc ggcacccgca cgaggacgag gcgcgggttg atcttcgaga gccgctccca
     1441 ggtgatgccc tgcttctcga tgttgggcgg caggttgttc tcgatgaggg cgtctgcctg
     1501 cgcgacgagg cgttcgaact gctcctggcc ctcggggcgg ctgaggtcga tggtcatcga
     1561 cttcttgcct cggccatgcc cgttgaagag cgcgttgcgg ttccacgggt cggccccggg
     1621 ctcgccgtcc gggtaccagc tcgcggccgc cgggttgttc gcgaccatcg cccgcgtcat
     1681 gtgcgcgagc tgaccgcgcg tgttcggcgc gaggtgctgt cgtgattcga tgcgaatgac
     1741 ctcggcgccc cagtcggcga ggtgcatcgt cgcgtaaggc ccggcccaga cgacggtgaa
     1801 atcgatcaca cgcaacccct caaggggcaa ccggtctcca aggcggtcct cgcccgtggc
     1861 aggagtcgtt gggcgggggc tgagttccgc cagcacctcg tccgtgtgct cgccgagcag
     1921 cggcgcacgc cgtcgaggcg gcatggggcc ctcgtccgtg tgcattcgga agtgccagcc
     1981 ggggtactgc gcgggtcccg tcgaggggtg gtcgatggtc tggaagaact cgcgggcgac
     2041 gaagttcggg tcggtaagca tgtcggccac ggtgttgagc ggcgcgccga ggacgccgta
     2101 cagctcgcag gcgtctcgga tttcccgctt cgtgtgcgag agcgtccagg gcagcaggta
     2161 cgccaggaac tcctcgatgc gctccgggtc agtcggcgcg ccgggggcct gccactcggg
     2221 gcgctccatc agctcttcgc agccaatcat gcgcatcgtc aggatcagcc ggccctgcaa
     2281 cccgccggtg agctgcacat aaccgtccgc gcagggaaag acgccgctgc cgaccagcga
     2341 ggccagcccc gagcgcgtgc cgatgcgccc cgagtactgg tacgcctgca gttgggtgag
     2401 ccgcatgtcg acgttgttcg tcagtgcctc gagcatgctc acgtcgacgt gctcgccgcg
     2461 gcctcgcagc tcggccgcct gcaccgcgac caccgtcgcg agcgccgcca tgtaagccgt
     2521 ctggtacgtc gccatccggc cggcgactcg gacgggttcg tgctcgatgt tgcccgtggc
     2581 cagcatgggg ccggccatcg cgtagacggt gaggtcggtc gcgagccagt cgcggtacgg
     2641 gccttcctgc ccgaagttgc tcagcgaggt gagcacgatg cgcgggttcg cctccgccag
     2701 gatgtcgtag cccaggccca ggcgctccat cgtgccgggg gcgaagttct ccaccacgac
     2761 ctgcgccgtg cgcacgaggt cgagcagtcg ctcgcgcccc tcggcggtcg tcagatcgag
     2821 gatcacgctg cgcttgttcg tgttcaggaa gaggaaggtg ccgctccgct cggtgccggg
     2881 ctcgtccttg acgaagggcg ggagctggcg tccgggatcg ccttcgggcg cctcgatctt
     2941 gatgacgtcc gcgccatagt caccgaggat gcgcgtgcag aacggtcccg ctgtgtaccg
     3001 cgtgagatcg atcacgcgca cgccctcgag aggcatcgtc gtcatgtatt gccccgtgtc
     3061 actgccggcg cctcaatcga ggcccccgca ccctgtgtac tcgtccgggc gcgggtggga
     3121 caagtggcgg cgcgcggggc gcgagacgcg tcgaggaacg aacttggccg gcgacgggcc
     3181 ggccaagttc gtgggaacgc gagttgtccg ctacttgtcg atccagaggt ccttcatctg
     3241 gatgcccgtg tcccagacat cgctgctgct gcccagcgcg ccgaagggac gcatgttccg
     3301 cacccacggc tgcatgaggt agaagccgtt cccgccgggg aacgcgaagc ggtacaccat
     3361 gtccatgtcg cggtcgtaga tctgtttgat cagcgcgcgc cgcttggccg ggtcgagctc
     3421 cacccgctgc ttctgcgaca ggtcgtcaat gacggggtca ttgatgagcc agcggttctg
     3481 cggcgacttc gagtagagct ggtcgtagac gtaggggtcg ggcgtcgtcc cgctcacgcc
     3541 ccagcccgtg atggtcgcct cttcgatctt gcggcccgtg agcaccgagg tgtacgcggt
     3601 gtactcgtcg gtgcgcaggt tgagggtgat gccggacttg cggtactggt cgacgagcag
     3661 ctcgccgtcc tgtttccagg ttggcaggta gtcgtagtgc acctcgttga tcgcgaggtc
     3721 tttcacaccc gcggcgtcga ggagctgctt cgactgcgcc gggtcgtact tcatccactt
     3781 gccgagctgg tcgccctttg gttcctgctc gaacgcgaac aaccagggga tgaggccgtg
     3841 tacgcggccg tagcccccat acacgatctg aatcatctcg tcccggttca cggcgaggga
     3901 cagcgctcgc cggacgcgct cgtccttgaa cttcgggttg tccgggttca tcaggaagcc
     3961 atggccacct gaggtgacgc ggctcatctg cacctgcacg ttcgggatcg tgtccgcgat
     4021 cgacttcgtg tcgcggtagg agctggcgat gttgccggcg tggtcgagct ggcccgctcg
     4081 caacgccgcg atgcgggcat tgaggtcgac gattacgcgt acctcgccgc cgtcgacgtt
     4141 gggcgcaggc ccccagtagt cggggttctt cacgaaggtg atgcgctggt cttcctgctt
     4201 gtccacgatg aacgccgcgg tgccgacgat cgtcttctgg atcgtcccgg cgtcgaccgt
     4261 ctccttcggg aagattggcg cctctcgacg cggcggcgtg acggtcagcc agtccaccag
     4321 cggctggtcg agcgacacct tcacggtgcg cgggtcgacc gcgtcgatgc tcttgacgcc
     4381 cacgaagaag ctcgcggccg cgccgctcgc cttcatgcgc tcgtaggcgt acttcacgtc
     4441 ctcggccacg aaagggcggc cgttcagcgg cggccggttc tgccacttcg cgttcgcgcg
     4501 cagcttgaag gtgatggcga gcccgtccgg cgaacgctcc caggactccg cgaggccagg
     4561 gatcacatcg actttcgtcg tgtctgccgt cggccccatc ttgtagccga gcagtcggtc
     4621 gtacacgggc gcgaactgga tcagcgtgcc gccggtgagg ttgaccgtcg ggtctgccgg
     4681 cgtgaactgg aagttgcttc cgtggacgaa gctgccgccc ttcttcgcgg tcccggcggg
     4741 gtccggaaac tcgtacgggt acggcatgtc ggcgtccttg acgaggcggc ccgggccggt
     4801 ggccgccgcg gtccccgtcg ccgcggcagt
//
LOCUS       DAORPG010000065         4821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1169_length_4821_cov_3.296055, whole genome shotgun sequence.
ACCESSION   DAORPG010000065 DAORPG010000000
VERSION     DAORPG010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1169_length_4821_cov_3.296055"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(338..1078)
                     /locus_tag="PLX85_02380"
     CDS             complement(338..1078)
                     /locus_tag="PLX85_02380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC62054.1"
                     /translation="MTAAEQPQVVVVTGAGAGIGRAIALEFGRSGARVAVAARTRSDI
                     EAVAAEIAGAGGTALPAAIDVKDPGSVEATLRDIRRALGPIDVLVNNAGAPGPRAFTQ
                     DVTPAEFLEVIQTNLWGAFLCTKFVLPAMVAGRRGCIINMTGGGAASDRPLRGGIAYA
                     SSKAGVEGFTRNLALEVSALGIAVNAIQPGRVDTRGFPAATITSGAGNLVPPDHAARL
                     AVWLTTDEGRQLSGQTIDAVEWDRARGA"
     gene            complement(1358..1433)
                     /locus_tag="PLX85_02385"
     tRNA            complement(1358..1433)
                     /locus_tag="PLX85_02385"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1398..1400),aa:Arg,seq:tcg)
     gene            complement(1584..2708)
                     /locus_tag="PLX85_02390"
     CDS             complement(1584..2708)
                     /locus_tag="PLX85_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HRC62055.1"
                     /translation="MTGSHERPDADALLRKLEREETAATRGRLRIYLGAAPGVGKTYA
                     MLHEGKRRAQRGTDVVVGFAETYGRPLTVDALTGLEVIPRRKVDYRGVTLEEMDVEAL
                     IARHPQVALVDELAHTNAPGSKHEKRWQDVQDIQAAGITVISTLNIQHLESLNDVVAG
                     ITGIHVRETVPDSIIDDADEIELVDVSAQALRSRMQHGNIYPPEQAQRALDGFFRESN
                     LTALRELVLRRVADQVEHELDEYMHEHALDSWEATERVLVLLDNIEGSEVAVRRAWRL
                     ASAFDGELFVAYPAASIRQQGMTRMLEVATDLNATVREIPGDHPAAELGDLIRTQKVS
                     HLVLVANPRRTLLGLRRKDLIDDMLEQTPNVSIHLVSQAS"
     gene            complement(2785..3648)
                     /locus_tag="PLX85_02395"
     CDS             complement(2785..3648)
                     /locus_tag="PLX85_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HRC62056.1"
                     /translation="MISDNGTTAAGKSRENGQLEPETTEAVAILKRWFVSRWSGLLAV
                     SIAAAVFTAIFTSFQDERRLPDVAMLYLAVTLVSAATWGYAVGAFSALLTNLFLNYFF
                     IPPLHVFNVADRGNAGGLVLFFLVAALGASLVSRMRDVARHLQTACEENLTLSELIHA
                     VAASAPGAEPSAFCSSAARRMGAARVELIEHRDGWVVLAGSARAGQYLDERESAAIAQ
                     LADRLSSGQRSTLKRIDDRFVVPLPGYTAPAVLVISGMDGALRSEDVDRFLRTVADQA
                     TIAIRLGRRAA"
     gene            complement(3687..4283)
                     /gene="kdpC"
                     /locus_tag="PLX85_02400"
     CDS             complement(3687..4283)
                     /gene="kdpC"
                     /locus_tag="PLX85_02400"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HRC62057.1"
                     /translation="MTAKALTQELRPLLGITVTMLLLTTAVYPLAVTGVAQTLFHRQA
                     NGSIVEVNGSPVGSTLIGQTFEGDQYFHGRPSAAGDGYDASASGGSNLGPTSQKLIDR
                     VTEDAAAFRQSNSLEAQATLPSDAVTASGSGLDPHVSPATARLQVERVAHARRVTEDQ
                     VRELVDEFTEGRQLVLIGEPRVNVLKLNIALDERFPLP"
     gene            complement(4295..>4821)
                     /locus_tag="PLX85_02405"
     CDS             complement(4295..>4821)
                     /locus_tag="PLX85_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRC62058.1"
                     /translation="QGRLVAMTGDGTNDAPALAQADVGVAMNSGTQAAKEAGNMVDLD
                     SDPTKLIEIVAIGKQLLMTRGALTTFSIANDVAKYFAILPAAFAVTYPALDALNVMHL
                     HSPESAILSAVIFNALIIVALVPLALRGVAYRAIGAASLLRRNALIYGVGGIIAPFVG
                     IKAIDLLLTAVNLA"
BASE COUNT          772 a         1498 c         1709 g          842 t
ORIGIN      
        1 gttcgcgagg cccctggagc aggcgggagc gatgcccctg gccgttgcgg ccggcgacgg
       61 gggcggcgcg gcagcgtctt cgtaacgacc gattcgtttt cgagggccac tacgaaacga
      121 aggaaacgaa tcgcggattc tgaatctgcg tctggttcgt ttcgttcgtt tcattcgttt
      181 tcggggtgtt cttcagccac gggcggcccg ggaactgccg gggtctcgtg gggattcgga
      241 tgacgggcgg cgtgaagcgc atgcggcgat ggtaccgagg gccgcgtcga tcacctcgga
      301 cgggatggca tcgagcttcg gtcccgcagg gtggcggtca ggcgccgcgg gctcgatccc
      361 actcgacggc gtcgatggtc tgaccgctga gctggcggcc ctcgtcggtg gtgagccaga
      421 ccgcgaggcg tgcggcgtga tcgggcggga cgaggttgcc ggcgccgctt gtgatggtgg
      481 cggcgggaaa gccgcgtgtg tcgacgcggc cgggctggat agcgttgacg gcgatgccga
      541 gggcggagac ttcgagggcg aggttgcgcg tgaacccttc gacgccggcc ttggaggagg
      601 cataggcgat gccgccgcgg aggggccggt cagaggccgc gccgccgccc gtcatgttga
      661 tgatgcagcc gcgccggccg gcgaccatgg cggggaggac gaacttggtg cagaggaagg
      721 cgccccagag gttggtctgg atgacctcga ggaactcggc gggggtgacg tcctgggtga
      781 aggcgcgcgg gcccggcgcg cccgcgttgt tcacgaggac gtcgattgga ccgagtgcgc
      841 ggcggatgtc gcggagcgtg gcctcgacgg agccgggatc cttcacgtcg atcgccgccg
      901 gaagtgcagt gccgcccgcg ccggcgatct cggcggctac cgcctcgatg tcggagcgcg
      961 tgcgggcggc gacagcgacg cgcgcaccac tgcggccgaa ctcgagggcg atagcccgcc
     1021 cgatgccggc gcccgccccc gtgaccacga ccacctgagg ctgttctgcg gctgtcatct
     1081 cgtgcccctc gatgtctgtg cgttcatcga gagcactgta gcgggcctac agcccgcccg
     1141 ggagctgcgc gggaggggag gtcgatgagg caggtcgggt gccggtcagc ttcattgggc
     1201 cgagggtgtg tacccgggga ggacgcggcg acctcgacga tctgctcgac gaaggcgtgc
     1261 gtgggtagtc aggacgagca tcctcgtcgt ttcacaccgc acgcaggttt ggtcacggag
     1321 cggcgggact gcacggtgtc cgccgaggca ggggggctgg cgcgccggga gggattcgaa
     1381 cccccgaccc tcagttccga agactgatgc tctatccact gagctaccgg cgcgagagac
     1441 agatttcaga ttcgaacgta cgcgacgcgg gacgccttgt gccgatcata gtcgaggcga
     1501 cgaagtcgcg cagcgcaggg ctggtgggtc cggcacccag cgcgcctcgg gctgtaacgg
     1561 gtcgctggcg gtgcgtctta aagctacgat gcctggctca cgaggtggat actcacgttc
     1621 ggcgtctgct ccagcatgtc gtcgatgagg tccttcctgc ggagacccag gagcgtccga
     1681 cgcgggttcg cgacgagcac gaggtgactc actttctggg tccggatgag atcgcccagc
     1741 tctgccgccg ggtggtcccc ggggatttcc ctgaccgtgg cgttgaggtc cgtcgccacc
     1801 tcaagcatgc gcgtcatccc ctgctggcgg atggacgccg ccgggtaggc gacgaacagt
     1861 tctccgtcga aggcgcttgc caggcgccac gcacggcgaa ccgctacttc cgagccttcg
     1921 atgttgtcga gcagcacgag cacccgttcc gtcgcctccc agctatcgag cgcgtgttcg
     1981 tgcatgtact cgtcgagctc gtgttcgacc tggtcggcga cgcgtcgaag gaccagctcc
     2041 cgcaatgcgg tcaaattgct ttctcgaaag aagccatcga gggcccgctg ggcttgctcg
     2101 ggcgggtaga tgttgccgtg ctgcatgcgg gagcggagcg cctgcgccga gacatcgacg
     2161 agttcgatct cgtcagcatc atcgatgatg gaatcgggta ccgtttcgcg gacgtggatg
     2221 ccggtgatgc cagctacgac atcgttcagg ctttcgaggt gctggatgtt gagggtcgag
     2281 atgaccgtga taccggccgc ctgaatatcc tgtacgtcct gccatcgttt ctcgtgtttc
     2341 gaaccgggcg cattcgtgtg ggcgagctcg tcaacgagcg cgacttgcgg gtggcgcgcg
     2401 atgagcgcct cgacgtccat ctcctcgagc gtcacgcccc gatagtcgac tttccgccgt
     2461 ggaatcacct cgaggccggt gagtgcgtcc acggtcagtg gccgaccgta agtctccgca
     2521 aagccaacga cgacgtccgt gccccgctgc gcccgccgct tgccctcgtg cagcatcgcg
     2581 taggtcttgc cgacgccggg cgcggcgccg aggtagatgc gaagccgacc gcgggttgct
     2641 gcggtctcct cgcgttcgag tttcctcagc agcgcgtccg cgtctgggcg ttcgtgggag
     2701 ccagtcatgg tgtttctggt gcgatcgacc cgcgccgcgc ggcgccaacg cccgtgatgt
     2761 cccgatccgc cgggagggag cggatcacgc tgcgcgcctt ccgagtcgga tcgcaatcgt
     2821 tgcctggtcc gcgacggtgc gcaggaagcg gtcgacgtcc tcagatctga gcgccccgtc
     2881 catccctgag atcaccagca cggcaggcgc agtgtaaccg ggcaacggga caacgaagcg
     2941 atcgtcgatc cgcttgaggg tcgaccgttg gcctgagctg agccgatctg cgagttgagc
     3001 gatagcggcc gattcgcgct catcgaggta ctgtcccgcc cgggcgcttc ccgcgagcac
     3061 cacccagccg tctcgatgct cgatgagttc cactcgggcg gctcccatac gtcgagcggc
     3121 cgaggagcag aacgcggagg gttccgcgcc gggtgctgag gcggccactg catgaatgag
     3181 ctccgacagg gtcaggttct cctcgcacgc ggtttggagg tgtcgcgcga cgtctcgcat
     3241 tcgggacacg agggacgcgc ccagcgcggc caccagaaag aagaggacaa gcccgcccgc
     3301 gttgccccgg tcagcgacgt tgaacacatg caacggtgga atgaagaagt agttgaggaa
     3361 gaggtttgtc aaaagcgcgc tgaacgcgcc caccgcgtag ccccacgtcg ccgcggagac
     3421 cagcgtgacc gccaggtaca gcatggctac atcgggaagg cgtcgctcat cctggaacga
     3481 cgtaaagatg gctgtgaaga cagctgccgc gatgcttacg gccagcaacc ctgaccagcg
     3541 actaacgaac cagcgcttga ggatcgcgac cgcctcggtc gtctctggtt ccaactgacc
     3601 attctcacga ctcttccccg cggcggtagt cccattgtcc gagatcatga cgcctccaat
     3661 tccgttgggc gttctgcttc gaggtgctac gggagtggga accgctcatc gagggcaatg
     3721 ttgagtttca gcacgttcac gcgaggctcg ccgatgagca ccagttggcg accctcggtg
     3781 aactcgtcca cgagttcgcg gacctggtct tcggtcaccc gtcgggcgtg cgccacacgc
     3841 tcaacctgga ggcgcgccgt ggcagggctg acgtggggat ccagtccgct gcccgacgca
     3901 gtgaccgcgt cggatggcag ggttgcctgc gcctccaggc tgttcgactg ccggaaggcc
     3961 gccgcatcct cggtcactcg atcgatgagc ttctggctcg tggggccgag gttcgacccg
     4021 ccggacgcgc tcgcatcgta gccgtcaccc gcggccgacg gacgcccatg gaagtactgg
     4081 tctccctcga acgtctggcc gatcagcgtg gagccgaccg gggacccatt cacctcgaca
     4141 atgctgccgt tggcctgccg gtggaacagg gtctgagcga cgcccgtgac ggccaggggg
     4201 tacacggcgg tggtgagcaa cagcatggtg acggtgatgc cgagcagggg cctgagctcc
     4261 tgggtgagag ccttggcagt catgatgtcc tccgctacgc caggttcacg gcggtaagca
     4321 gcaggtcaat cgctttgatc ccgacgaagg gagcgatgat ccctccaacg ccgtagatca
     4381 aggcgttccg cctcaagagg gacgccgcgc cgatggcgcg gtaggcgacg ccgcggagcg
     4441 caagcggtac gagagcgacg atgatgagcg cgttgaagat caccgccgag aggatcgcgc
     4501 tttcgggcga gtgcagatgc atcacgttca gggcgtcgag ggcggggtag gtcacggcga
     4561 aggcggcagg gaggatggcg aagtacttcg cgacgtcatt ggcgatgctg aaagtggtca
     4621 gcgcgccgcg tgtcatgagc agctgctttc caatcgcgac gatctcgatg agcttggtcg
     4681 ggtcgctgtc gaggtccacc atgttgcccg cctctttggc ggcctgggtc ccgctgttca
     4741 tggcgacgcc cacgtccgcc tgggccagcg ccggggcgtc attcgtccca tcgcccgtca
     4801 tggcgaccaa acggccctgc g
//
LOCUS       DAORPG010000066         4788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1192_length_4788_cov_3.159096, whole genome shotgun sequence.
ACCESSION   DAORPG010000066 DAORPG010000000
VERSION     DAORPG010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1192_length_4788_cov_3.159096"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1377)
                     /locus_tag="PLX85_02410"
     CDS             complement(<1..1377)
                     /locus_tag="PLX85_02410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC62059.1"
                     /translation="MRFPIPLTQARPSVALAAGSVDGAARVAGPSERVVVVPVYDERA
                     TVIGVLDDVVALGFERLLIVDDGSTDGSSELLDEWAVAHPSARVIHLPENRGKAAALR
                     AAWDVLRRDLERGDLGPGTAVIGIDADRQHDLRQVGALVDRLETLGVDAVIAQRDLSY
                     HRPFKRFGNHVMAAIGSVLGGGRLHDVESGFRVVRLGPLLHATEFYRGRRYSEASELA
                     VVLKRLGYRVDNDYVVQVPVARSRTHLSDAASHIVAMTGAWFRVVCWSTVPQSLRWRV
                     NVALAAVLLIVFTGFLGMLLTHPVFLGDDSAQSYGHVWFIAKRLYAGEWLPLHMASLE
                     SGRAYTLPYGALPWIPTALLRPLLGDWAVTASMGVGVALLFLGIWRWLPRTATPLMTA
                     LVLLNWQLWNGILQFQLLTIWALAFACLAAAEYDRSRPRRGTALAFAAFLAHPLMGGC
                     ALAVTSLAA"
     gene            1721..2143
                     /locus_tag="PLX85_02415"
     CDS             1721..2143
                     /locus_tag="PLX85_02415"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRC62060.1"
                     /translation="MPIDLTDFAEAIDNARADGTPCIVATQGSEGVPDIGIKGSMMVF
                     DREHLAFWERALGQNFANLQATPGIAVIYFNRERGKLIRAFGHVELHADGPVREQIMS
                     RTIEAELNMDPERKGVGVLIRVDRFVEAFSRTTQLRDE"
     gene            2172..2531
                     /locus_tag="PLX85_02420"
     CDS             2172..2531
                     /locus_tag="PLX85_02420"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRC62061.1"
                     /translation="MTRNPDVDRWLEERAHPLDVAMRRARDIILATDDRITESIKWNT
                     PTFEFHGNIVSFNPSKKLVSLMFHRGAEIPGHHPRLEGDGKLVRTMRLASLEDVEAAR
                     PELEAAIRAWCDWKAGA"
     gene            2566..3144
                     /locus_tag="PLX85_02425"
     CDS             2566..3144
                     /locus_tag="PLX85_02425"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HRC62062.1"
                     /translation="MRIGLISDTHIPEARPELWPQVFEAFRGVEAILHAGDIHDVGVI
                     DQLERIAPTYAARGNGDDGSGGRPVQPDDPRLREVWEIELGGLRIGMLHVAPIPAYGL
                     YTLERALDRHFGHMDLDVLVYGDTHVEAIDTLHGILCVNPGSPTFPRNHTTRLGTIGF
                     LDVVDGRPEASIWQLTEHGHEPYDWSTWRNPW"
     gene            3214..3648
                     /locus_tag="PLX85_02430"
     CDS             3214..3648
                     /locus_tag="PLX85_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRC62063.1"
                     /translation="MSCEGDTAEELRHTGRRFTVQRLKVAAALRHCGGHRTAEEIHDL
                     LAAQDPHASMPLSTVYRTLATLKEARLVAEVDNGGPAAYEWVAEQPHHHLICQRCEAD
                     LDLDPRLLADLAGRIEAETGFEAYLDHLAIQGRCAHCRDHGA"
     gene            3645..4703
                     /locus_tag="PLX85_02435"
     CDS             3645..4703
                     /locus_tag="PLX85_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HRC62064.1"
                     /translation="MRSRRLVALAVTMGAAVALLLTACGSKSDEAVGDGLRGVQSSFE
                     GNDGRLKVATTVAPLTSIVLNVGGDRIRIHQLIPDGVDSHTFEPKPSDAKVLSRADLL
                     IMNGAHLEGTTEKIAKQNLPHPEKIYYLADNTLQGDDERSGFLYDFSFPRSEGDPNPH
                     LWMNPLYALRYAELTAQWLGENDPDNRDYYQQNLARFADVLKRIDAAIQQDQASVPQQ
                     NRRLLTYHDSWAYWARRYGWTVIGAAQPSDFKEPSAQEVGNLVAQIKKEGVPAVFGSE
                     VFPSKTLESIARETGAKFEDRLRDDEPPGAAGDAQHTYVGMLVEDMRIMLTALGGNAD
                     ETSKIPVANTFERGRSSS"
BASE COUNT          826 a         1842 c         1476 g          644 t
ORIGIN      
        1 cgccgcgagc gaggtgaccg cgagcgcgca gccccccatc agcggatggg cgaggaacgc
       61 cgcgaacgcc agcgcggtgc cgcggcgtgg ccggctgcgg tcgtactccg ccgccgccag
      121 gcacgcgaac gccagcgccc agatcgtgag cagctgaaac tgcaggatgc cgttccacaa
      181 ctgccagttc agcaacacca gcgcggtcat cagcggcgtc gcggtccgcg gcaaccagcg
      241 ccagatcccg aggaacaaca gcgcgacgcc gacccccatg ctcgccgtca ccgcccagtc
      301 gccgagcagc ggccgcaata gcgccgtcgg gatccacggc agcgccccgt acggcaacgt
      361 gtacgcgcgg cccgattcga ggcttgccat gtgcagcggc agccactcac ccgcgtagag
      421 acgcttggcg ataaaccaca cgtgcccgta ggactgcgcg ctgtcgtccc ccaggaacac
      481 cgggtgcgtg agcagcatgc cgaggaaccc ggtgaacacg atcagcagca ccgccgccag
      541 cgccacgttg acgcgccagc gcagcgactg cggcaccgtg ctccagcaca ccacccggaa
      601 ccacgccccg gtcatcgcga cgatgtgcga tgccgcgtcc gaaagatgcg tccgcgatcg
      661 cgccaccggc acctgcacga cgtagtcgtt gtcgactcga tacccgagcc gcttcagcac
      721 taccgccagc tcgctcgcct cgctgtaccg ccgcccccga tagaactcgg tcgcgtgcag
      781 cagcggcccg aggcgcacga ctcgaaaccc gctctccacg tcgtgcagcc gcccgccccc
      841 cagcacgctg ccgatcgccg ccatcacgtg gttcccgaac cgcttgaacg gccggtggta
      901 cgagaggtcg cgctgcgcga tcaccgcgtc gacgcccagc gtctcgaggc ggtcgaccag
      961 cgcccctacc tgtcgcaggt catgctgccg gtccgcatcg atcccgatca ccgctgtgcc
     1021 cgggccgagg tcgccccgct cgaggtcgcg ccgcaacacg tcccaggccg cgcgcaacgc
     1081 cgccgccttg ccgcggtttt cgggcaggtg gatcacccgc gccgagggat gcgccacggc
     1141 ccactcgtcg agcagctccg acgacccgtc ggtcgacccg tcgtccacga tgagcagccg
     1201 ctcgaacccc agcgccacga catcgtcgag caccccgatg accgtggccc gctcgtcgta
     1261 cacggggacg accacaacgc gctccgacgg accagcgacg cgcgcggcac catcgacact
     1321 ccccgccgcg agcgccacgg acggacgtgc ttgcgtcagc gggatgggaa acctcatcgc
     1381 agctcggtgt aaacaggcgc ctgcgtctgg ggcaatgtcc ggaccgcctc gccccactca
     1441 ccattctggg cacccaccgc ccgatgctcc acctcgcatg cggcggaacg cctccggtta
     1501 gtggccttgg gagggggact acggtcagat cgggaagccg gtacaagagg cgcgtactgg
     1561 cgaccacatg gaaggccggc ctaccggtgg ccggccttcc gcgcgcccgg cgcgggctcg
     1621 gcgacggcgc tgcggcgaga tgcacggtgt tagactcgcg ggcggctcgc gatctcgcgc
     1681 gctcacctta cgcggcagca cgtttcgagg gaggacttgc atgcccatcg atctcaccga
     1741 cttcgccgag gccatcgaca acgcccgcgc cgacggcacc ccctgcatcg tcgccacgca
     1801 gggctccgag ggcgtcccgg acattggcat caagggctcg atgatggtct tcgaccggga
     1861 gcacctcgcg ttctgggagc gcgccctcgg ccagaacttc gccaacctcc aggccacccc
     1921 cggcatcgcc gtcatctact tcaaccgcga gcgcggcaag ctgattcgcg ccttcggcca
     1981 tgtcgagctg cacgccgatg gccccgtccg cgagcagatc atgtcccgca ccatcgaggc
     2041 cgaactgaac atggacccgg aacgcaaggg cgtcggggtg ctcatccgcg tcgaccgctt
     2101 cgtcgaagcg ttcagccgga cgacccaact tcgcgacgaa tgagctagtc ggccgcacga
     2161 ggggatccag gttgacgcgc aaccctgacg tcgatcgctg gctcgaggag cgtgcgcacc
     2221 cgctcgatgt cgccatgcgt cgcgcccgcg acatcatcct cgcgaccgac gaccgtatca
     2281 ccgagtcgat caagtggaac acgccgacct tcgagttcca cggcaacatc gtgagcttca
     2341 acccctcgaa gaagctcgtc agcctgatgt tccaccgcgg cgcggagatc cccggccacc
     2401 acccgcgcct cgagggcgac ggcaagctcg tccgcacgat gcgcctcgca agcctcgagg
     2461 atgtcgaggc ggcccgcccc gagctcgagg cggcaatccg cgcctggtgc gactggaagg
     2521 ctggcgcctg atccggccgc cgtccgctcc cgctacactc cccgcatgcg catcggcctg
     2581 atctccgaca cccacatccc tgaggctcgg cccgagctct ggccccaggt cttcgaggcg
     2641 ttccgcggcg tcgaggccat cctccacgcc ggtgacatcc acgacgtcgg cgtcatcgac
     2701 cagctcgagc gcatcgcccc cacctacgcc gcgcgcggca acggcgacga cggctccggc
     2761 ggccgccccg tccagccgga tgacccccgc ctccgcgagg tgtgggagat cgagctcggt
     2821 gggctccgca tcggcatgct ccacgtcgcg ccgatccccg cctacggcct ttacaccctc
     2881 gagcgcgccc tggaccgcca cttcggccac atggacctgg atgtcctcgt ctacggcgac
     2941 acccacgtcg aggccatcga caccctccac ggcatcctct gcgtgaatcc cggctcgccg
     3001 acgttccccc gcaaccacac gacgcgcctc ggcacgatcg gcttcctcga cgtggtggac
     3061 ggccgtcccg aggcctccat ctggcagctc accgagcatg gccacgagcc atacgactgg
     3121 tcgacctggc gcaatccctg gtaacccacg cgagcccaca acccgttcgc ctcgcgctac
     3181 acttgcgaat attcgcaaga gggagcgcgc gcgatgagtt gtgagggcga caccgccgag
     3241 gagctccgcc acaccgggcg ccgctttacc gtccagcgcc tcaaggtcgc cgccgccctc
     3301 cggcactgcg gcggacaccg caccgccgag gagatccatg acctcctggc cgcgcaggac
     3361 ccgcacgcct ccatgccgct ctcgacggtc taccgcacgc tggcgacgct gaaggaggcc
     3421 cgcctcgtcg ctgaggtgga caacggcggc cccgccgcct acgagtgggt cgccgagcaa
     3481 ccgcaccacc acttgatctg ccagcgatgc gaagccgacc tcgacctcga cccgcgcttg
     3541 ctcgccgacc tcgccggtcg tatcgaggcc gagaccggct tcgaggccta cctcgaccac
     3601 ctcgccatcc agggccgctg cgcgcactgc cgggaccacg gcgcatgagg tcccgacggc
     3661 tcgtcgcgct cgccgtgacg atgggggcgg cggtcgcgct gctgctcacc gcctgcggct
     3721 caaagagcga cgaagccgtc ggcgacggcc tccgaggcgt ccagagcagc ttcgagggca
     3781 atgacggacg cctgaaagtc gccaccacgg tggcgccgct gaccagcatc gtcctgaacg
     3841 tcggcggcga ccgcatccgc atccatcagc tcatccccga tggcgtcgac tcccacacct
     3901 tcgagccgaa gccctcggac gcgaaggtcc tctcccgcgc cgacctcctg atcatgaacg
     3961 gcgcgcacct cgaggggacc accgagaaga tcgcgaagca gaacctcccc cacccggaga
     4021 agatttacta cctcgccgat aacaccctgc agggcgacga cgagcgctcg ggtttcctct
     4081 acgacttcag cttcccccgc agcgagggcg acccgaaccc acacctctgg atgaacccgc
     4141 tgtacgcgct ccgctacgcg gaactcacgg cgcagtggct cggtgagaac gaccccgaca
     4201 accgcgacta ctaccagcag aacctcgcga ggttcgctga cgtcctgaag cgcatcgacg
     4261 ccgccatcca gcaggatcag gcgtccgtgc ctcagcagaa ccgcaggctg ctgacctacc
     4321 acgactcctg ggcctactgg gcgaggcggt acgggtggac ggtgatcggt gcggcgcagc
     4381 cctccgactt caaggagccg agcgcgcagg aggtcggcaa cctcgtggcc cagatcaaga
     4441 aggaaggcgt ccccgccgtc ttcggcagcg aagtcttccc cagcaagacc ctggagtcga
     4501 tcgcccgcga gaccggggcg aagttcgagg accgcctgcg cgacgacgag cccccggggg
     4561 cggccggcga cgcgcagcac acatatgtcg gcatgctggt cgaggacatg cggatcatgc
     4621 tcaccgccct cggaggcaac gccgacgaga cctcgaaaat cccggtcgcc aacactttcg
     4681 agcgaggccg gtcctcgtca tgagtgcggt catcgacctc cgcgacgtga cctgcggcta
     4741 cagcggccgc cccgtcctgc aaggcgtcaa cctgaccgtc gaggaggg
//
LOCUS       DAORPG010000067         4786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1193_length_4786_cov_3.834919, whole genome shotgun sequence.
ACCESSION   DAORPG010000067 DAORPG010000000
VERSION     DAORPG010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1193_length_4786_cov_3.834919"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /gene="dnaA"
                     /locus_tag="PLX85_02440"
     CDS             <1..1144
                     /gene="dnaA"
                     /locus_tag="PLX85_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018936430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRC62065.1"
                     /translation="LADGGLPVSIEVGSREVPSPAPAAPTLPTASGQPRGAPALVGVP
                     LNANFTFASFIEGKSNHFAKAAAQQVAENPGSAYNPLFIYGGVGLGKTHLMHAIGHLI
                     RERKPDARIAYVHSERFVSDMVRALQHNMINEFKATYRSLHALLIDDIQFFAGKDRSQ
                     EEFFHTFNSLIEGQHQVILTCDRYPKEVEGLEERLKSRFGWGLTVAIEPPDLETCVAI
                     LLGKAQLAGVELPEDVAFFIAKCIRSNVRELEGALRRVIANARFLGRPITLEFAREAL
                     KDLLQLKARLITVENIQKTVADYYKVRIADLLSKRRSRSVARPRQVAMALAKELTTHS
                     LPEIGDAFGGRDHTTVMHACKRIRELRESEPRMQDDFAILLRTLTG"
     gene            1346..2443
                     /gene="dnaN"
                     /locus_tag="PLX85_02445"
     CDS             1346..2443
                     /gene="dnaN"
                     /locus_tag="PLX85_02445"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018918394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRC62066.1"
                     /translation="MKLSATRETLLGPLQSVMGVVERRQTMPVLANVLLSARGEKLTV
                     TGTDLEVELVAAAAVSIQQPGDVTVPGRKLLEIMRTLPEKSTVSLVREAERVVVKAGR
                     SRFTLSSLPASEFPVIDDINARQGIRVARAAFRRLIEKTHFAMAQQDVRYYLNGTLLE
                     ASGKTLRAVATDGHRLSLAETELAEAIEANQQVIVPRKGILELQRILSGDGELELSIG
                     SNHVRAQVGDIRFTSKLIDGKFPEYGRVIPSKPPHVCTAARDVLRQALQRTSILSNEK
                     YRGVRLAFAANQLTIQAHNPEQEEAEDQVEVGYQGAELEIGFNVTYLLDALAAIDVDT
                     VEIALTDSNSSCLMRAPGDLQVKFVVMPMRL"
     gene            2462..3517
                     /gene="recF"
                     /locus_tag="PLX85_02450"
     CDS             2462..3517
                     /gene="recF"
                     /locus_tag="PLX85_02450"
                     /inference="COORDINATES: protein motif:HMM:NF000819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HRC62067.1"
                     /translation="MSLQTLELDNLRCIERAELELHPRLNLVTGPNGSGKTSLLEAVH
                     LLGRGRSFRTRLTERLVRFGSDWLNVVGRVEDPLVGTVGVHFHRETGLRARMAGRDVR
                     SLGELSVALPVMILDPGIHRLVEEGPSYRRRWLDWGVFHVEPAFAAAWASYTQSLRQR
                     NAALRQGADPTAWEPELARAGELITECRRRTLEQLEAHWVPVAQRLLGLPISLTFYRG
                     WSSERSLAQGLELGRARDVETGGTGTGPHRFDVWLRIEGRAAREVLSRGQQKLLGACM
                     ALAITRLVTGIRGKPPVLLLDDPAAELDATRTQLLIEEIRATSGQLLVTALHAEDLRF
                     GTPERTFHVEQGRVTTL"
     gene            3545..>4786
                     /locus_tag="PLX85_02455"
     CDS             3545..>4786
                     /locus_tag="PLX85_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC62068.1"
                     /translation="MTNEEVYDSSKIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFE
                     VVDNAIDEALAGHCDRICVTIHADESVTVSDNGRGIPVDIHPEEGRSAAEVIMTVLHA
                     GGKFDDSSYKVSGGLHGVGVSVVNALSDTLLLTICRDGRMHRQEYRLGEPQAPLAEVG
                     ETSERGTVVRFKPSAQIFTNIQFVYDTLAKRLRELSFLNSGVRIELVDEREDKHDVFQ
                     HEGGLRAFVQHLGRGRTAIHDSVFWFRAQEGNIGVEVAMQWNDSYQESMYCYTNNIPQ
                     KDGGTHLSGFRAALTRTLNDYIEKEMAAKKEKIATTGDDAREGLTAILSVKVPDPKFS
                     SQTKDKLVSSEVKGVVETAVANRLSEFLLENPAQAKAVVAKIIDAARAREAARKAREM
                     TRRKGALDVAGLPGKLADCQEK"
BASE COUNT          858 a         1591 c         1609 g          728 t
ORIGIN      
        1 gctcgccgac ggcggcctgc cggtcagcat cgaggtgggc tcgcgcgaag tgccctcgcc
       61 cgccccggcg gcgccaacgc tgcccacggc cagcggccag ccgcgcggcg cgcctgccct
      121 cgtcggcgtg ccgctcaacg cgaacttcac cttcgcgtcc ttcatcgagg gcaagagcaa
      181 ccacttcgcc aaggccgcgg cgcaacaggt ggccgagaac ccgggttcgg cctacaaccc
      241 gctgttcatc tacggcggcg tcggcctcgg caagacgcac ctgatgcacg cgatcgggca
      301 cctgatccgc gagcgcaagc ccgacgcgcg catcgcctac gtgcattccg agcgcttcgt
      361 ctccgacatg gtgcgcgcgc tccagcacaa catgatcaac gaattcaagg cgacctaccg
      421 ctcgctccac gcgctcctga tcgacgacat ccagttcttc gccggcaagg accgctcgca
      481 ggaagagttc ttccacacct tcaactcgct gatcgaaggc cagcaccagg tgatcctcac
      541 ctgcgaccgc tacccgaagg aggtcgaggg cctggaagaa cgcctgaaat cgcgcttcgg
      601 ctggggcctc acggtcgcga tcgagccgcc cgacctcgag acctgcgtgg cgatcctgct
      661 cggcaaggca cagctcgccg gcgtcgagct gcccgaggac gtggccttct tcatcgccaa
      721 gtgcatccgc tcgaacgtgc gcgagctcga gggcgcattg cgccgcgtga tcgcgaacgc
      781 ccgcttcctc ggccggccga tcacgctgga attcgcgcgc gaggcgctca aggacctgct
      841 gcagctgaag gcccgcctga tcaccgtcga gaacatccag aagacggtcg cggactacta
      901 caaggtccgc atcgccgacc tgctttccaa gcgccgcagc cgttcggtcg cgcggccgcg
      961 ccaggtggcg atggcgctcg cgaaggaact gacgacgcac agcctgcccg agatcggcga
     1021 cgccttcggc gggcgcgacc acacgacggt catgcacgcg tgcaagcgca tccgcgagct
     1081 gcgcgagagc gagccgcgga tgcaggacga cttcgcgatc ctgttgagaa ccctgaccgg
     1141 ctgaggtgga agaagctgtg ggttcttttg tggacaatac tgtccacagc cggcccacag
     1201 cctgcacccg cgcaacccgc cgccggccgg tgatggcgcc gaccgcggat tttccgggcg
     1261 cggggcctgc aaggagcggt tgtccccgat ccccacaggc ctaataatcg taactagtct
     1321 taattcccaa gaagaagatc cagatatgaa actctccgcg acgcgtgaaa ccctgctcgg
     1381 acccctgcag agcgtgatgg gcgtggtcga gcgccggcag acgatgccgg tcctggcaaa
     1441 cgtgctgctg agcgcgcgcg gcgagaagct gacggtcacg ggcaccgacc tcgaggtgga
     1501 actggtcgcg gcggcggcgg tgagcatcca gcagccgggc gacgtgacgg tgccgggccg
     1561 caaattgctg gagatcatgc gcacgctccc ggagaagtcg acggtctcgc tggtgcgcga
     1621 ggccgagcgc gtggtcgtga aggccggccg cagccgcttc acgctctcga gcctgccggc
     1681 gagtgaattc cctgtcatcg acgacatcaa cgcgcgccag ggcatccgcg tcgctcgtgc
     1741 ggccttccgc cggctgatcg agaagacgca tttcgcgatg gcgcagcagg acgtgcgcta
     1801 ctacctcaac ggcacgctgc tcgaggccag cggcaagacg ctgcgcgcgg tggcgaccga
     1861 tggccaccgg ctgtcgctgg ccgagaccga actggccgag gcgatcgagg cgaaccagca
     1921 ggtcatcgtg ccgcgcaagg gcatcctgga gctgcagcgg atcctcagcg gcgacggcga
     1981 gctcgagctc agcatcggat cgaaccatgt tcgtgcgcag gtcggcgaca ttcgcttcac
     2041 ttcgaagctc attgacggca agttcccgga atatggccgc gtgatcccgt ccaagccccc
     2101 gcatgtgtgc actgcagcac gagatgtact gcgccaggcc ctgcagcgca cgtcgatcct
     2161 ctcgaatgag aaataccgcg gcgttcgcct cgcgttcgct gccaatcagc tcacgatcca
     2221 ggcacataac cccgagcagg aagaagccga agaccaggtc gaggtcggtt accagggtgc
     2281 cgagctcgaa atcggcttca acgtgaccta cctgctcgat gccctggcgg cgatcgacgt
     2341 cgatacggtc gagatcgcgc tgaccgattc gaactccagc tgcctcatgc gggcgccggg
     2401 cgacctgcag gtcaagttcg tcgtcatgcc gatgaggctc tagggctacg accagggctc
     2461 cgtgagcctc cagacgctcg agctcgacaa cctccgctgc atcgagcgag ccgagctcga
     2521 gctgcatccg cggctcaatc tcgtgaccgg gccaaacggc tcgggcaaga ccagcctgct
     2581 cgaggccgtg catctgctcg ggcgcggacg gtcgttccgc acgcgcctga ccgagcgcct
     2641 ggtgcggttc gggagcgact ggctcaacgt agtcggccgc gtcgaagacc cgctggtcgg
     2701 cacggtgggt gtgcatttcc accgcgagac ggggctccgg gcgcggatgg cgggccgtga
     2761 cgtgcgctcg ctgggcgagt tgtcggtcgc gctacccgtc atgatcctgg atcccggcat
     2821 ccatcgcctg gtggaggagg ggccgtcgta ccggcgccgc tggctggact ggggagtgtt
     2881 ccacgtggaa cctgccttcg ctgccgcctg ggccagctat acccagtccc tgcggcagcg
     2941 gaatgcggcc ctccggcagg gggctgaccc cacggcctgg gagccggagc tggcgcgcgc
     3001 gggcgagctg atcacggaat gccggcgccg gacgctggag cagctggagg ctcattgggt
     3061 gccggtggcc cagcgcctgc tggggctacc catctcgctg acgttctacc gcggctggtc
     3121 ttccgagcgc tccctggccc agggcctgga actggggcgc gcccgcgatg tcgagactgg
     3181 cggtaccggc acgggaccgc atcgcttcga cgtctggctg cgcattgagg gtcgggcggc
     3241 gagggaggtg ctgtcgcgag gccagcagaa gctgctgggg gcctgcatgg cgttggcgat
     3301 cacgcgcctg gtcacgggca tccggggcaa gccgccggtg ctgctgctgg acgatccggc
     3361 ggctgaactc gacgccacgc gcacgcagct gttgatcgag gaaatcaggg cgacgagcgg
     3421 gcagttactg gtcacggccc tgcacgcgga ggacctgagg ttcgggacgc cggagcgtac
     3481 gtttcacgtg gaacaaggtc gcgttaccac gctataataa cagtatgcgt tccggggcga
     3541 tcgaatgacc aacgaagaag tctacgattc cagtaaaatc aaagtcttaa agggtcttga
     3601 tgcggtgcgc aaaagaccgg gcatgtacat cggtgacacc gatgacggca gcggcctgca
     3661 ccacatggtc ttcgaggtcg tcgacaacgc gatcgacgag gcgctcgcgg ggcattgcga
     3721 ccgcatctgc gtgacgatcc acgcggacga atcggtcacg gtcagcgaca acggccgcgg
     3781 cattccggtc gacatccatc ctgaagaagg gcgctccgcg gccgaggtca tcatgaccgt
     3841 gctgcacgcc ggcggaaaat tcgacgacag ttcctacaag gtttcgggcg gactgcatgg
     3901 cgtcggcgtc tcggtcgtca acgcgctttc cgacacgctg ctgctgacga tctgccgcga
     3961 cgggcgcatg caccgccagg aataccgcct gggcgagccg caggcgccgc tcgccgaggt
     4021 cggcgagacg agcgagcgcg gcacggtcgt gcgcttcaag cccagcgcgc agatcttcac
     4081 caacatccag ttcgtctacg acacgctggc caagcgcctg cgcgagctgt ccttcctgaa
     4141 ttcgggcgtc cgcatcgagc tcgtcgacga gcgcgaggac aagcacgacg tgttccagca
     4201 cgaggggggc ctgcgcgcct tcgtgcagca cctgggccgc ggccgcaccg cgatccacga
     4261 cagcgtgttc tggttccgcg cgcaggaagg caacatcggc gtcgaggtcg cgatgcagtg
     4321 gaacgattcc taccaggaat cgatgtactg ctacacgaac aacatcccgc agaaggacgg
     4381 cggcacgcac ctgtcgggct tccgcgccgc gctgacgcgc acgctgaacg actacatcga
     4441 gaaggagatg gcggcgaaga aggaaaagat cgccacgacc ggcgatgacg cccgcgaggg
     4501 cctcacggcg atcctgtcgg tcaaggtgcc cgacccgaag ttctcctcgc agaccaagga
     4561 caagctggtt tcatccgagg tcaagggcgt ggtcgagacg gccgtcgcca accggctgag
     4621 cgagttcctg ctcgagaacc cggcgcaggc gaaggccgtg gtcgcgaaga tcatcgacgc
     4681 ggcgcgggcg cgcgaggccg cgcgcaaggc gcgcgagatg acgcggcgca agggcgcgct
     4741 cgacgtcgcg gggctccccg gaaagctcgc cgactgccag gaaaaa
//
LOCUS       DAORPG010000068         4766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1204_length_4766_cov_3.490979, whole genome shotgun sequence.
ACCESSION   DAORPG010000068 DAORPG010000000
VERSION     DAORPG010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1204_length_4766_cov_3.490979"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="PLX85_02460"
     CDS             complement(<1..1371)
                     /locus_tag="PLX85_02460"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC62069.1"
                     /translation="MAFSAVGYERDGGAMLAAARSEGMRRLLRGVYFGYWVVATLFVA
                     EIAVTGISFYSFSLFIREWQHDEDLRWSLTAINASFWVGLPLAFFTPLIGRGIDTLGP
                     RRMMLFGVPLVGLAFIVESMMTQLWQLWLGQGLLVVGQSAAFVGTGKLIGMWFERDRG
                     RMMGIALAGNNAGGIIMGRAVPRLFDAFGWRWTLGGAGIIVTLLNLVLIWLFIRDHPR
                     DVLEASDGRPARASEAAVARSMLANDTRSGLRWPGLGGLLAAPFVLTLRTFREVWTKG
                     MASWTFWLLTLTSVTSFVSIYAVLNQLGKHLELVGIDKTTTGTAITILGACGLLGKLI
                     FGYLSERIPSRVAFGTVCLVQVVGIAVLLGVRSPDQTALLWVFAVIYGVGFGAVGAVQ
                     PLVVADSFGLLAFGVVFGSLQLLLRGVQSAIPFFVGRSVDATGSYEAAFVVTMVALCL
                     GAVSAMF"
     gene            complement(1427..3196)
                     /locus_tag="PLX85_02465"
     CDS             complement(1427..3196)
                     /locus_tag="PLX85_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC62070.1"
                     /translation="MSDNPFEERASLQGRLARRSVLRGGGLLIGGVAAAALIGCGSDD
                     EEQAPPSATSSAAASTAPGKGELIRDNALPYPYQFPEPNKVPKAGGTMVLGVNSDVSI
                     MDPQKTTASGTGRIVNLVYNRLLGYNRGVRLHPFKFAIEGELAKSWEQSPDGLVYTFK
                     LQDGVKWQNIAPLNGRPFVADDVKFAFDRYKSGGISTSYWVGVQTIEAPDPKTVKITL
                     KKPLADFLTPLGSAYQTIHPKELVDSGEIEKKAVGTGPMILKDAIVGQKVTFEKNPEY
                     WEGKVLLDGVEFRLQVDTPARLAAFRSGQTDFGDILVTNITDAKALESTNQGVQINMS
                     SVVLASGFAMNLSNPKFQDVRVRRAISLGIDRQALANLVYPGIGKILPMHPWIFHLDK
                     EPTTDSFGQWHQFNPNDATKLLAAAGASNLELQNKYYPYSQAYEQTNDVMVDQLRKVG
                     VNLVGGKVEYTEYNSQWTGRKLPEVSTGGWVTIGFDADNYYYNCMHSSAAGNRWNLND
                     PKVDDLASRQQSEVNPQKRKDLLKQIYEYDLDQAYHPIMATGVGFYAYQPWLHGIRFG
                     GAMGTSNNYQDIGDMIMDAWIDK"
     gene            complement(3331..4650)
                     /locus_tag="PLX85_02470"
     CDS             complement(3331..4650)
                     /locus_tag="PLX85_02470"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRC62071.1"
                     /translation="MPSPADERDQPLAGVRILDLTVVWAGPYATMHLADWGAEIIRIE
                     SLHHFASFTRGTLARPSQALVDTSSTSGMGYPEDRAGEHPWNRSPVFNHHGRNKRSMT
                     LDLTRPEGQEVFERLVRESDGMIENNLPPHAEELGVTWDRLSKINPRFILMRVPGFGV
                     EGPYRDYRTFGNHMEAIAGHPVIRAYPDLGLDYAPASVPADAASGVGGALAFTLALRQ
                     RERTGKGVYVELATAENYVPFLSEFVLDYSMNGRVWEQMGNDHWTVAPHNAYPCQGMD
                     RWVTIACTSEDDWRALCGLMGRPGLATDERFADMPSRHANRRDLDAEISAWTREHDAH
                     WVTQRLQALGVAAGVVLHESELLRDRHLDHRGYWQTIEHPETGVQQNVGRLWRASDTP
                     HPPARHAPMLGQDNDYVYRELLGFSDEEYRAFIESGHIGTEYDASVP"
BASE COUNT          815 a         1658 c         1497 g          796 t
ORIGIN      
        1 gaacatcgcc gacaccgcgc cgaggcacag cgccaccatc gtgaccacga acgccgcctc
       61 atagcttccc gtcgcatcca ccgaccgccc gacgaagaac gggatcgccg actgcacccc
      121 acgcagcagc aactgcaacg acccgaacac caccccgaac gccagcaacc cgaacgagtc
      181 cgcgaccacg agcggctgca cggcgcccac cgccccgaac cccacgccgt agatcaccgc
      241 gaacacccag agcagtgccg tctggtccgg actccgcacg ccgaggagca ccgcgatccc
      301 cacgacctgc acgaggcaca ccgtcccgaa cgccacccgc gacgggatgc gttccgagag
      361 gtacccgaag atcagcttcc ccagcagccc acacgccccg aggatcgtga tcgccgttcc
      421 cgtcgtcgtc ttgtcgatcc ccaccagctc caggtgcttc cccagctggt tcagcaccgc
      481 gtaaatcgac acgaacgacg tgacggaggt cagcgtcagc agccagaacg tccagctcgc
      541 catccccttg gtccagactt cgcggaaggt ccgcagcgtc agcacgaacg gcgccgccag
      601 cagcccgccg aggcccggcc agcgcaggcc gctcctcgta tcgttcgcca gcatcgaacg
      661 tgcgaccgcc gcctccgatg cgcgcgccgg ccggccgtcg ctcgcctcca gcacatctcg
      721 ggggtgatcc cggatgaaca accagatcag cacgaggttc aggagcgtga cgatgatgcc
      781 cgcaccaccg agcgtccacc gccacccgaa cgcgtcgaac agccgcggca ccgcacggcc
      841 catgatgatg ccgccagcgt tgttccccgc gagcgcgatg cccatcattc gcccacggtc
      901 acgctcgaac cacatcccga tcagcttgcc cgtgccgaca aacgcggctg actgccccac
      961 gacgagaagg ccctgcccga gccacagctg ccacaactgc gtcatcatcg actcgacgat
     1021 gaaagccagc ccgaccagcg gcacgccgaa cagcatcatc cgccgaggcc cgagcgtgtc
     1081 gatgccacgg ccgatcagcg gcgtgaagaa cgccagcggc agtcccaccc agaacgaggc
     1141 gttgatcgcc gtcaggctcc agcggaggtc ctcgtcgtgt tgccactcac ggatgaagag
     1201 gctgaacgag tagaaactga taccggtgac cgcgatctcg gccacgaaca gcgtggcgac
     1261 gacccagtac ccgaagtaca ccccgcgcaa gagccgcctc attccctccg accgcgccgc
     1321 cgccagcata gcgccgccat cacgctcgta gccgacagcg gagaacgcca caaacgagac
     1381 gggcggcgca aatgccgccg cccgcctctc agcagacctc gcgcggctac ttgtcgatcc
     1441 aggcgtccat gatcatgtcg ccgatgtcct ggtagttgtt cgaggtgccc atcgccccac
     1501 caaagcgaat cccatgtagc cacggctggt aggcgtagaa gcccacgccc gttgccatga
     1561 tcgggtggta cgcctggtcg aggtcgtact cgtagatctg cttgagcaga tcctttcgct
     1621 tctgcgggtt cacctcggac tgctgccgtg aggccagatc gtcgaccttc gggtcgttga
     1681 ggttccagcg gttgcccgcc gccgatgagt gcatgcagtt gtaatagtag ttgtcggcgt
     1741 cgaagccaat cgtgacccag ccgccggtgg acacctcggg caacttgcgc ccggtccact
     1801 gcgagttgta ctcggtgtac tccacctttc cgccgacgag gttcacgccg accttccgca
     1861 gctggtcgac catcacatcg ttcgtctgct cgtaggcctg ggagtacggg tagtacttgt
     1921 tctgcagctc gaggttgctc gcgcccgcgg ccgccaacaa cttggtcgcg tcgttggggt
     1981 tgaactgatg ccactggccg aagctgtccg tcgtcggctc tttatcgagg tggaagatcc
     2041 acgggtgcat gggcaggatc ttcccgatgc ccgggtagac caggttcgcc agggcttgcc
     2101 ggtcgatgcc gagcgagatc gcgcgccgca cgcgcacatc ctggaacttc gggttcgaca
     2161 ggttcatcgc gaacccgctt gcgagcacga ccgagctcat attgatctgc acgccctggt
     2221 tcgtcgactc gagcgccttc gcgtcggtga tgttcgtgac caggatgtcg ccaaagtcgg
     2281 tctgcccgga gcggaacgcc gcgaggcggg cgggcgtgtc cacctgcagg cggaactcga
     2341 cgccatcgag taggaccttg ccctcccagt actcggggtt cttctcgaag gtgactttct
     2401 gcccgacgat cgcgtctttc aggatcatcg gtccggtgcc gaccgccttc ttctcgatct
     2461 cgcccgagtc gaccagctct ttcgggtgga tcgtctggta cgcggagccg agcggcgtga
     2521 ggaagtctgc cagcggcttt ttcagcgtga tcttgacggt cttggggtcg ggcgcctcga
     2581 tcgtctggac tccgacccag tacgaggtgc tgatgccgcc ggacttgtag cgatcgaacg
     2641 cgaacttgac gtcatccgcg acgaacggcc gcccgttgag gggggcgatg ttctgccact
     2701 tcacgccatc ctgcagcttg aacgtgtaca cgaggccgtc cggcgactgc tcccacgact
     2761 tcgcgagctc gccctcgatc gcgaacttga acgggtgcag gcgtacgcct cggttgtagc
     2821 cgagcagccg gttgtagacg aggttcacga tgcgcccggt gccgctcgcg gtcgtcttct
     2881 gaggatccat gatcgagacg tccgagttga cgcccaggac catggtgccg ccggccttcg
     2941 ggaccttgtt cggctcaggg aactggtacg ggtacggaag cgcgttgtca cggatgagct
     3001 cgcccttgcc gggcgccgtc gaggcggccg cggaagacgt agcagacggc ggagcctgct
     3061 cctcgtcgtc gctgccgcat ccgatcaggg cggccgcggc gacgccgccg atgagcaggc
     3121 cgccgccgcg gagcacgctc cgtcgtgcga gtctcccctg gagtgaggcg cgttcttcaa
     3181 agggattgtc tgacacggtt gctggatacc tcctctgcgt cgtacgaagt gcggcaatct
     3241 cgccgcccgc cctacacgca gacgtgatac cacatccgtt aagacgataa caatcggagg
     3301 tgcacatcga ggccgtcgcg gtcccggccg tcagggaacc gaagcgtcgt actccgtgcc
     3361 gatgtggccc gactcgatga acgcccggta ctcctcgtcg ctgaagccaa ggagctcgcg
     3421 gtacacgtaa tcgttatcct ggccgagcat cggtgcatgg cgggccggcg gatgcggagt
     3481 gtcgctcgct cgccataggc gccccacgtt ctgctgcaca ccggtctcag gatgctcgat
     3541 cgtctgccaa taccctcgat ggtcgaggtg gcggtcgcgc agcagctccg actcgtggag
     3601 cacgacgcct gccgcaacgc ccagtgcctg caaccgctgg gtgacccagt gcgcgtcgtg
     3661 ctcgcgcgtc catgcggaga tttccgcgtc cagatctcgg cgatttgcgt gcctcgaggg
     3721 catgtccgcg aagcgctcgt cggtggcgag gccggggcgg cccatcagtc cgcagagcgc
     3781 gcgccagtcg tcctcgctcg tgcatgcgat cgtgacccag cggtccatcc cctggcacgg
     3841 gtaagcgttg tgcggggcca ccgtccagtg gtcgttgccc atctgctccc acacgcggcc
     3901 gttcatggag tagtcgagga cgaactcgga caggaacggc acgtagttct cagccgtcgc
     3961 cagctcgacg tacacgcctt tccccgtgcg ttcccgctgc cgcagcgcga gcgtgaaggc
     4021 gagcgccccg ccgacacccg aggcggcgtc ggccggcacg ctggccggcg cgtagtccag
     4081 gccgaggtcg gggtacgcgc ggatgaccgg gtgtcccgca atcgcctcca tgtggttccc
     4141 gaaggtgcgg tagtcccgat atgggccttc gaccccgaag ccgggcacgc gcatgaggat
     4201 gaaccgcggg ttgatcttcg acagccggtc ccacgtcacg ccgagttcct cggcgtgcgg
     4261 cggcaggttg ttctcaatca tcccgtccga ctcgcggacg aggcgctcga acacctcctg
     4321 cccctcaggc cgggtcaggt cgagggtcat tgaacgcttg ttgcgcccgt ggtggttgaa
     4381 gacgggcgac cggttccagg ggtgctcgcc ggcgcggtct tccggatacc ccatgccgct
     4441 ggtcgaggag gtgtcgacca gtgcctgcga cggccgcgcc agcgtgccac gcgtgaagct
     4501 ggcgaagtgg tgcaacgact cgatgcggat gatctctgca ccccagtccg cgaggtgcat
     4561 ggtggcatag ggccccgccc acaccaccgt gaggtcgaga atgcgcaccc cggcgagagg
     4621 ctggtcacgc tcgtcagctg gcgacggcat cgcggacctc ctcgcgcgtc tggatcacgc
     4681 cggcaccgcg cagccggtcg atatcagcgg gttgcatacc gagcacgtcg cgaagcacct
     4741 cgacggtgtg ttcgccgagc agcggc
//
LOCUS       DAORPG010000069         4738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1220_length_4738_cov_3.306641, whole genome shotgun sequence.
ACCESSION   DAORPG010000069 DAORPG010000000
VERSION     DAORPG010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1220_length_4738_cov_3.306641"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PLX85_02475"
     CDS             complement(<1..492)
                     /locus_tag="PLX85_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRC62072.1"
                     /translation="MSFTSRVFQVDTLDLGDGKQEQIVKGGRHLFPLLPKAFEGISQI
                     GVIGWGSQGPAQAQNLRDSLAGTAIKVAVGLRPGSSSMAEARDAGFSEEDGTLGEMFE
                     VVRNSDLVLLLISDAAQAELFEQVFANLKQGATIGLSHGFLLGHMKNVGKSFPAHVNA
                     IAVC"
     gene            complement(729..929)
                     /locus_tag="PLX85_02480"
     CDS             complement(729..929)
                     /locus_tag="PLX85_02480"
                     /inference="COORDINATES: protein motif:HMM:NF022716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3072 domain-containing protein"
                     /protein_id="HRC62073.1"
                     /translation="MASNPAGRPMTEAQREYLKLLSEQAEEPFDDSLDEEAATRRIEE
                     LREVTGKGVHDRDPDEPVERPE"
     gene            complement(1033..1932)
                     /gene="dapA"
                     /locus_tag="PLX85_02485"
     CDS             complement(1033..1932)
                     /gene="dapA"
                     /locus_tag="PLX85_02485"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRC62074.1"
                     /translation="MTEIGRLLTAMVTPFRADGSVDYEQAGELAKALLASGSDGIVLT
                     GTTGETPTLSIEEQVEVWRSVRRAVGPDAMLVGGASTNSTSESLEMVTQGVDAGMSAL
                     MFTVPYYNKPPQEGLFRHFRTLAEATPLPCILYNIPGRTSLNMTAETTLRLAREVPNI
                     VGIKEASGDMEQIGIILDGAPAGFKVWAGDDSAAVNIVERGGWGVISVASHLVGRQIS
                     EMLAAAAAGQIEEARRLEAGLMPLFNVLFIETNPIPVKFALNHVGFRVGGLRLPMIEA
                     SEAAQAKVSAELAKHQVDLPIPV"
     gene            complement(2069..3088)
                     /locus_tag="PLX85_02490"
     CDS             complement(2069..3088)
                     /locus_tag="PLX85_02490"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRC62075.1"
                     /translation="MSREPNVAVIGASGAVGDVFLRVAEERNFPVGNLKLLATARSAG
                     KKLRFRGEEITVEETTAEALKGADLVFCSATSAASREWAPVITAGGGIMIDDGSAFRM
                     QANVPLVVPEINGDDLEWHERIVSIPNCTTTPLVMALHAMRQVAALKRVTVATYQAVS
                     GTGVAAIQELRAQLEAYGRGEPMPAPEVYPQQIALNVIPQVDVFGEDGYTAEEAKMRN
                     ETRKILHEPELPFSATCVRVPTIIGHAEAVQLEFQSAAALDDLREALRQQPGMVLMDE
                     PSKSVYPTPLNTAGDDRAFVGRLRIDPSVEHGVAFWCVTDNLRKGAATNAIQIAEELI
                     RRGKV"
     gene            complement(3151..3279)
                     /locus_tag="PLX85_02495"
     CDS             complement(3151..3279)
                     /locus_tag="PLX85_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62076.1"
                     /translation="MHMYDVPTMQQTTGHELIANAALRAPQGGTPALVTTTTKKQS"
     gene            3430..4191
                     /locus_tag="PLX85_02500"
     CDS             3430..4191
                     /locus_tag="PLX85_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013020501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC62077.1"
                     /translation="MSWFEHGEARVYYEESGVGEPLLLLPGWAGSIDELAHVRAALEG
                     SYRVIAADLPGSGKSAPQPRTFTPRYLEEDANTFLALLGELGVASAHLVGFSDGGEYA
                     LLMAATRPEVARSVVTWGAAGSLGTNTQMADAMTALIDDPIPPMAGFADFLKAAYGED
                     NARIMTRSAGQAFRAIMEKGGDVSRSRAGRIKAPALLITGEHDFLATPDLVADMAAAI
                     PKATFVQAEGASHPVHHEQPEWLMKTVVDWLRERS"
     gene            complement(4205..>4738)
                     /locus_tag="PLX85_02505"
     CDS             complement(4205..>4738)
                     /locus_tag="PLX85_02505"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRC62078.1"
                     /translation="AIQGTAEALPFLDRSFDACLAVLTVHHWSDGAAGLKELRRVARD
                     RIVILTHDFRGIDLPWLVRDYFPGIVVQDRERFAPIEEVVADLGGARVEAVPIPADCA
                     DGFMLAHWRQPERYLDASVRAAMSGFLAIPEHEVETGVARLRADLDSGEWRRRNAALL
                     ELDAYDGGYRLIVAELR"
BASE COUNT          754 a         1705 c         1515 g          764 t
ORIGIN      
        1 gcacacggcg atcgcgttca catgcgccgg gaatgacttg ccgacgttct tcatgtgccc
       61 gagcaagaac ccgtgcgaca gaccgatcgt cgcgccctgc ttcaggttcg cgaacacctg
      121 ctcgaacagc tccgcctgtg ccgcgtccga gatgagcagc agcacgaggt cggagttccg
      181 caccacctcg aacatctcgc cgagggtgcc gtcctcttcg gagaacccgg catcgcgcgc
      241 ctcggccatc gaggacgacc cgggtcgcag tccgacggcg accttgatag cagtgccggc
      301 gagtgaatcg cgcaggttct gcgcctgggc cggcccctgc gagccccacc cgatcacgcc
      361 aatctggctg atgccctcga acgccttcgg gagcagcggg aacaggtgtc ggccgccctt
      421 cacgatctgc tcctgcttgc cgtcgccgag atccagcgtg tcgacctgga agacgcgtga
      481 agtgaaggac atcgaggaac cccctgcgta caccgttcga gtgacgttcg atgaacaccg
      541 gggcgtcgct acgcactcac ctcgaggcgg accgcgcgct ccggcgacca ctcacgttag
      601 cgggacacgc gtcctcagtc gacatgcagg gggaatcgac tgccgcccgg acgcgaacgg
      661 cagcgagaga acggcttcgg gccgggcctc gagaggaggg ctcgagcgtc cggcccgagg
      721 cctcggggct attccggtcg ctccaccggc tcatccgggt cgcggtcgtg cacgcccttc
      781 cccgtcacct cgcgcagctc ctcgatccgc cgcgtcgcgg cctcctcgtc gaggctgtcg
      841 tcgaagggtt cctctgcctg ctcgctgagc agcttcaggt actcgcgctg ggcctccgtc
      901 atcggacggc ccgccggatt actggccatc gcagacctcc tcgctcactc gccccgtcag
      961 gctagtgagc gggaggtcac gtctgaggaa cgaggcggcc ctgcgggctc tcggcctcgt
     1021 tacgtctcac gactagaccg ggattggcag gtcgacctgg tgcttcgcca gctccgcgga
     1081 caccttcgcc tgtgccgctt ccgaggcctc gatcatcggc aaccgcagcc cgcccacgcg
     1141 gaacccgacg tggttgagcg caaacttcac agggatcggg ttcgtctcga tgaacaggac
     1201 gttgaacagt ggcatgagcc cggcttcgag gcggcgcgcc tcctcgattt gcccggcggc
     1261 ggctgcagcc agcatctcgc tgatctgccg tccgacgagg tggctcgcga ccgagatcac
     1321 accccacccg ccgcgctcga cgatgttcac ggccgcgctg tcgtcgccag cccacacctt
     1381 gaaccccgcg ggcgcgccat cgaggatgat cccgatctgc tccatgtccc ctgaggcttc
     1441 cttgatcccg acgatgttgg gcacctcgcg agccagccgc agcgtggtct ctgccgtcat
     1501 gttcaatgag gtgcggccgg gaatgttgta gaggatgcac ggcagtggcg tcgcctcggc
     1561 cagcgtccgg aagtgccgga acagcccctc ctgcggcggc ttgttgtagt acggcaccgt
     1621 gaacatgagc gcgctcatcc cggcgtccac gccctgcgtc accatctcga gcgactctga
     1681 agtcgagttc gtcgaggcgc cgccgacaag catcgcgtcc gggcccacgg cgcggcggac
     1741 ggaccgccac acctcgacct gctcctcgat gctcagggtc ggcgtctcgc cggtcgtgcc
     1801 ggtgagcacg atgccgtccg aacccgaggc gagcagcgcc tttgcgagct cgccggcctg
     1861 ctcatagtcc acagacccgt ccgcgcggaa cggagtcacc atcgccgtca gcagccgccc
     1921 gatctcagtc atcgcacata cctccccccg gaagacgggg tgttcgccgt cccacgggcg
     1981 gcgcggtcag aacgcaggaa tggtaccgcg cccgacgcaa ccccggggcg gctgatccga
     2041 ggagcccccg ccgcctttgc ggcgagggct agaccttgcc tcgccggatc agttcctcgg
     2101 cgatctggat ggcgttcgtg gccgcgccct tgcgcaggtt gtccgtcacg caccagaacg
     2161 cgacgccgtg ctctaccgac gggtcgatgc gcagccgccc aacgaacgcc cggtcgtcgc
     2221 ccgccgtgtt cagcggtgtc gggtagacgg acttcgaggg ctcatccatc agcaccatcc
     2281 ccggctgctg ccgtagcgcc tcgcgcaggt catcgagggc cgctgcgctc tggaactcca
     2341 actgcactgc ctcggcgtgc ccgatgatcg tcggaacgcg cacacacgtc gccgagaacg
     2401 gcagctccgg ctcgtggagg atcttgcgcg tctcgttgcg catctttgcc tcttccgccg
     2461 tgtagccatc ctcgccgaag acgtccacct gcgggatgac gttgagcgcg atctgctgcg
     2521 ggtacacctc gggcgccggc atcggctcgc cacgcccata cgcctcgagc tgtgcgcgca
     2581 gctcctggat cgccgcgacg cccgtgcccg acaccgcctg gtacgtggcc acagtcacgc
     2641 gcttcagcgc cgcgacctgc cgcatcgcgt gcagcgccat caccagcggc gtcgtcgtgc
     2701 agttcgggat cgacacgatc cgctcgtgcc attcgaggtc gtcgccgttg atctccggca
     2761 ccaccagcgg cacgttcgcc tgcatccgga acgccgaccc gtcgtcgatc atgatcccgc
     2821 ccccggcggt gatcaccggg gcccactccc tcgaggccgc cgaggtcgct gaacaaaaga
     2881 cgaggtccgc gcccttgagc gcctccgctg tcgtctcctc gaccgtgatc tcctcgccac
     2941 ggaaacgcag cttcttcccc gcggagcgag cggtcgcgag gagcttcagg ttgccaacgg
     3001 ggaagttgcg ctcctcggcg acgcggagga acacgtcccc cactgcgccg gaggcgccga
     3061 tcacagcgac gttcggttct cgagacacgg ggacaccctc ctgccggccc ctgcgtcgag
     3121 gagcagggca cctcgcacag ggggtgtgta ctagctctgc ttcttggtgg tggtcgtgac
     3181 gagcgcgggc gtgccgccct gcggcgctcg aagcgcagcg ttggcgatca gttcatggcc
     3241 ggtcgtctgc tgcatagtcg gcacatcata catatgcacg acgaaacagc gtaggggcgc
     3301 tgcgagcggc ccggtgccga cgaaagaaac tcgtcagaac tcgcctccac ccgaagcgta
     3361 tgctcccgtc tgcctgccct acctatcggt tggcgaggga agcggggcgg catagaggag
     3421 gcgaccgcaa tgtcctggtt cgagcacggt gaggcgcgcg tgtactacga ggagtccgga
     3481 gtcggcgagc ccttgctcct gctgcccggc tgggccggca gcatcgacga actggcccat
     3541 gtccgcgcag ccctcgaagg cagttatcgc gtgatcgcgg ccgaccttcc gggctctgga
     3601 aagtccgctc ctcagccccg gaccttcacc ccccggtacc tcgaggaaga tgcgaacaca
     3661 ttcctcgccc tgctgggcga gcttggtgta gcctccgccc acctcgtcgg cttcagtgac
     3721 ggcggcgagt atgcgttgct catggcggcg actcgacccg aagtcgcccg gtccgtggtg
     3781 acctggggcg cggcggggtc actcgggacc aacacgcaga tggcggacgc catgaccgcg
     3841 ctgatcgacg atccgatccc gccgatggct ggcttcgccg atttcctgaa ggcggcctac
     3901 ggcgaggaca acgcccgcat catgacccgc agcgctggac aggcgttccg agccatcatg
     3961 gagaagggcg gcgacgtgag ccgctcccgc gccggacgca tcaaggctcc ggcgctgctc
     4021 atcaccggcg agcacgactt cctcgcgaca cccgacctgg tcgccgatat ggctgccgcg
     4081 atccccaaag cgaccttcgt ccaggcggag ggtgccagcc accccgtcca ccacgagcag
     4141 cccgagtggc tcatgaagac cgtggtcgac tggctccgcg aacggtcgtg acctcgcgcg
     4201 gcggctatct cagctcggcg acgatcagcc ggtatccgcc gtcgtacgca tcgagctcca
     4261 gcagcgcggc gtttcgccgc cgccactccc cgctgtcgag gtcggcgcgg agccgcgcca
     4321 caccggtctc gacctcgtgt tcaggaatgg cgagaaagcc cgacatcgcc gcgcgtacgg
     4381 acgcatcgag gtagcgctcc ggctgccgcc aatgagccag catgaaaccg tccgcacagt
     4441 cagcggggat cggcacggct tctacgcgcg ctccgccgag gtcggcaacg acttcctcga
     4501 tcggcgcgaa ccgctcccga tcctgcacga cgatgccggg gaagtagtcg cgcacgagcc
     4561 agggcaagtc gatgccccgg aaatcgtgcg tcaggatcac gatgcgatcg cgtgccaccc
     4621 ggcgcagctc cttgaggcca gcagctccat ccgaccagtg gtgcacggtc agcacggcca
     4681 ggcaggcgtc gaagctccga tcgaggaacg gcagcgcttc cgccgtaccc tgaatggc
//
LOCUS       DAORPG010000070         4653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1270_length_4653_cov_3.544802, whole genome shotgun sequence.
ACCESSION   DAORPG010000070 DAORPG010000000
VERSION     DAORPG010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1270_length_4653_cov_3.544802"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..1029
                     /locus_tag="PLX85_02510"
     CDS             202..1029
                     /locus_tag="PLX85_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62079.1"
                     /translation="MARLYLRFDEASLDLDHSDEWEAVLFPLGRIANVADARNVDYDA
                     RDLRSEAPAGASYAFSDAPLGESSFFRDLERDLKDQVTRAQRMEVQVNRKLKLAARAG
                     ETPEEFAKRCDQAGQNAADAEAAKLRDRYDAKADRIRDAINRAEDRVDVLKTDTSTRR
                     TSEILGAAGDLLGAFLGGRHTTRSMASGAGRILRGASSRRGQSARTSQRLDVAESQLQ
                     DRQEDLDELEAELAREIAEINDRWADVARDVETVEVPLEKTDVSVQEVALAWIPVGN"
     gene            1110..1442
                     /locus_tag="PLX85_02515"
     CDS             1110..1442
                     /locus_tag="PLX85_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HRC62080.1"
                     /translation="MQVPFELTTASNGQFRFSLRTAEGDTLLNSETYVQKASAQNGIE
                     SVQSNSGNEARYDRLTASDGRAYFNLKAGNGQVIGTSPMFATEAARDAAIAATMTEGS
                     STRIDDKT"
     gene            1535..2290
                     /locus_tag="PLX85_02520"
     CDS             1535..2290
                     /locus_tag="PLX85_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC62081.1"
                     /translation="MADFEGRVAVVTGAAHGIGRATALAFGRAGASVVVADHGDAGEA
                     VAEEVRASGAEAVFVRVDVARAPEVAALMEATEARFGRIDCLFNNAGIEGQQAPTAEA
                     TLENWDRVIAVNLGGVFYGMKYAIPALLRAGGGAIVNNASVAGLVGFAGASAYTASKG
                     GVVQLTRTAALEYAAQGIRVNCVCPGVIDTPMVERVIQGSPAVRDAMASMQPLGRMGT
                     PEEIAQTVLFLSSDAASFITGGVFPVDGGYVAR"
     gene            complement(2335..3192)
                     /locus_tag="PLX85_02525"
     CDS             complement(2335..3192)
                     /locus_tag="PLX85_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRC62082.1"
                     /translation="MTSDRYGSNVSAPIERVPLPDWPHKELLYETIDNGRIALITMNR
                     PERMNSSDPDMGFRWYDAWTRFANDDEQWVAIVTGAGDRAFSAGQDLKIRAELEEQGG
                     DAPYQRRAPNVTPIGEKLGCWKPTIAAINGFAIAGGWATAQNCTFRIADEHAEMNIAE
                     ARWNQAAGFVAWLPRLMNTSHALEVCIMGDRRISAQRAYEMGFVNKVVPKGECVNEAV
                     DWARTICRMAPRTVRNFHQMVYQCFNMTIPEAQAFASALEFNLRGMEDSLEGPKAFAE
                     KRPAVFKNR"
     gene            3344..3712
                     /locus_tag="PLX85_02530"
     CDS             3344..3712
                     /locus_tag="PLX85_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRC62083.1"
                     /translation="MTDPLSLTFAALADPTRRAILARLTEGDAPLKELAAPFAMSLPA
                     VSKHLKVLERAGLIERGRDAQWRPAHLRGDALRQVDEWVEPYRRYWQTRLGYLDRYLE
                     ELQASAAATPGGEEGNDDRG"
     gene            3699..4190
                     /locus_tag="PLX85_02535"
     CDS             3699..4190
                     /locus_tag="PLX85_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRC62084.1"
                     /translation="MTAGDTVLRLPSDVEIVVMRQFRAPVALVFATWTQPEHLRRWFG
                     RDGWSLPVCEIDLRPGGAWRYVHLGPDGMELGLSGEYLEVDPPHRLVSTERFDEPYFE
                     AMGGGTVNTITFDEDDGGITTMTLRVLYASKEARDGALQTGMEEGMAEGFRRLDAHLA
                     GLA"
     gene            4215..>4653
                     /locus_tag="PLX85_02540"
     CDS             4215..>4653
                     /locus_tag="PLX85_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018383127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC62085.1"
                     /translation="MQKIHPMLWFNTQAEEAAEFYTSLFPDSRITDVARFGDGSPGTA
                     GTVMTVSFELAGQQLTALNGGPEFNFTEAISLYVDCADQAEVDRLWERLTDGGEPGQC
                     GWLKDRFGVSWQVIPSALGELMGDPDPERTGRVVQAMLQMTKID"
BASE COUNT          781 a         1480 c         1676 g          716 t
ORIGIN      
        1 cccgccgcga acgtgagcgc accctccagc acggccactg ccgccccggc ggcagtacct
       61 caaccgacag ctgccgcgtc agacgcgagc ctcgtcccgc caaccgtcgc cgctggcgtc
      121 gcatcgtatt atgtcgattc ggccgcgccg tggttggcag aggtcggcgg acggcctggt
      181 acccgactcg aacctgcgat cgtggcacgg ttgtacctgc ggttcgatga ggcgtcgctc
      241 gacctcgacc actcggacga gtgggaagcg gtgctgttcc cgctgggccg gatcgcgaac
      301 gtggcggacg cgcgaaacgt ggactacgac gcccgcgatc tacgcagcga agcgcccgcg
      361 ggcgcgtcgt acgcgttctc ggacgcaccg ctcggcgagt ccagcttctt ccgtgacctc
      421 gaacgcgatc tcaaggacca ggtcacgagg gctcagcgga tggaggtgca ggtcaaccgc
      481 aaattgaagc tggcggcgcg cgcgggagag acgccagagg agttcgcgaa gcggtgcgac
      541 caggccggac agaatgccgc cgacgctgag gctgcgaagc tgcgggaccg ctacgacgca
      601 aaggcagacc gcatccgaga cgccatcaac cgggctgagg accgcgtcga cgtcctgaag
      661 acggacactt cgacacggcg gacctcggag atcctcgggg cggccggtga cctgctcggc
      721 gcgttcctcg ggggccggca cacgacgcgg tcgatggcga gcggggcggg gcgcatcttg
      781 cgcggggcgt cttcgcggcg gggccagagc gcgcgcacct cgcaacggct ggacgtggcg
      841 gagtcacagc tgcaggaccg ccaggaggat ctcgacgagc tggaggccga gctcgcgcga
      901 gagatcgccg agatcaacga ccgctgggca gacgtggcgc gcgacgtcga gaccgtcgag
      961 gtgccactcg agaagaccga cgtgtccgtg caggaggtcg cgctggcctg gatcccggtc
     1021 ggcaactaga cggcgccgcg gttccccggc ctctggtgag cccgcgagaa atgtgtcagc
     1081 atcgacgagc gggggcgagg aacaggagga tccaggtgcc attcgaactc acgaccgcga
     1141 gcaacgggca gtttcgcttc tcgctgcgaa ccgccgaggg cgacacgctc ttgaacagcg
     1201 agacgtacgt ccagaaggcg tccgcgcaga acgggatcga gtccgtgcag tcgaactcgg
     1261 gcaacgaggc gcgctacgac cgcctgacgg ccagcgatgg gcgcgcctat ttcaacctga
     1321 aggcgggcaa tggccaggtg atcggcacga gtccgatgtt cgcgaccgag gcggcgcgcg
     1381 acgccgctat cgccgcgacg atgaccgagg gctcgtcgac ccgtatcgac gacaagacgt
     1441 agccgcaggt gtcctcgtcg agcctcgagc cccgccgcct gccagtactg aggcgaggcg
     1501 gcggggacag cccgaacggc gaagaggtgt gctaatggcc gacttcgagg gacgcgtcgc
     1561 cgtcgtgacc ggggcggcgc atggcattgg gcgcgcgacc gcgctggcct ttgggcgcgc
     1621 gggagcgtcg gtcgtggttg cggaccacgg ggacgccggc gaagccgttg ccgaggaggt
     1681 tcgggcgagc ggggcggagg cggtgttcgt gcgcgtcgac gttgcgcgcg cgccggaagt
     1741 agccgcgttg atggaggcaa ccgaggcgcg cttcggccgc atcgactgcc tcttcaacaa
     1801 cgcgggaatc gaaggccagc aggcgccgac ggccgaggcg acgctcgaga actgggaccg
     1861 agtgatcgcg gtgaacctcg gcggcgtgtt ctatggcatg aaatacgcga tccccgcgct
     1921 gctgcgcgcc ggcggcgggg cgatcgtgaa caacgcctcg gtggccggac tcgtcggatt
     1981 cgccggcgct tccgcctaca ccgcctcgaa aggcggagtc gtccagctca cgcgaacggc
     2041 ggcgctcgaa tacgcggccc agggtatccg cgtgaactgc gtctgcccgg gcgtgatcga
     2101 tacgccgatg gttgagcggg tgatccaggg cagcccggcg gtccgtgacg cgatggcctc
     2161 gatgcagccc ctcgggcgta tgggaacgcc cgaggagatc gcgcagaccg tgctgtttct
     2221 gtcctcggac gccgcctcgt tcatcacggg cggtgtcttc cccgtcgatg gaggctacgt
     2281 cgcgcgctga cgcctgcgga gcgggcgaca cgcgcccgcc ccgcgggcga ggactcagcg
     2341 gttcttgaac accgcgggcc gcttctcggc gaaggccttg gggccctcga gcgagtcctc
     2401 catcccccgg aggttgaact caagggcgct cgcgaaggcc tgcgcctcgg ggatggtcat
     2461 gttgaagcac tggtagacca tctggtggaa gttgcgcacg gtgcgaggcg ccatgcggca
     2521 gatggtgcgt gcccagtcga cggcctcgtt cacacactcg cccttcggga cgaccttgtt
     2581 gacgaagccc atttcgtacg cgcgctgcgc gctgatgcga cggtcgccca tgatgcacac
     2641 ctcgagggca tgactcgtat tcatgaggcg gggcagccag gcgacgaagc ccgcagcctg
     2701 gttccagcgc gcctcggcga tattcatctc ggcatgctcg tccgcgatgc ggaacgtgca
     2761 gttctgtgcc gtagcccagc caccggcgat ggcgaagccg ttgatggcgg cgatcgtcgg
     2821 tttccagcag ccaagcttct cgccgatggg ggtgacgttc ggggcgcgac gctgataggg
     2881 cgcgtcgccg ccctgctcct cgagttccgc gcggatcttg agatcctgcc cggcggaaaa
     2941 ggcgcggtca cccgcgccgg tcacgatggc gacccattgc tcatcgtcgt tggcgaatcg
     3001 cgtccaggcg tcgtaccagc ggaagcccat gtcggggtcg ctcgagttca tccgctccgg
     3061 ccggttcatc gtgatgaggg cgatacggcc gttgtcgatg gtctcgtaga gcagctcctt
     3121 gtggggccag tctggcagcg ggacacgctc gatcggcgct gagacattcg aaccgtagcg
     3181 gtcgctggtc atgcaggtgc tccttccgta tcaaccaagt acctcgaata acaaccgcgt
     3241 atcgtgctgc agtgtagcgg agccgaaggg gccctcgcag gcttgacggg ccaggaatgt
     3301 tcacgtatac aaccttcagg ttaattaaca gataggttca tccgtgaccg acccgctcag
     3361 cctcacgttc gccgcccttg ccgacccgac gcggcgggcg atcctcgcga gactcaccga
     3421 gggcgacgcg ccgctgaagg agctggccgc gccatttgcg atgagcctgc cggcggtgtc
     3481 caagcacctc aaagtcctcg aacgcgcggg cctgatcgag cgcgggcgcg acgcacagtg
     3541 gcgtccggcc cacctgcggg gcgacgcgct gcgccaggtt gatgagtggg tcgagcccta
     3601 ccggcggtac tggcagacgc gacttggata cctggacaga tacctcgagg aactgcaggc
     3661 cagcgcggcg gctacgccgg gcggcgagga ggggaacgat gaccgcgggt gacaccgtgc
     3721 tgcgtctgcc ctcggacgtg gagatcgtgg tgatgcggca gtttcgagcg ccggtggcgc
     3781 tcgtattcgc aacgtggacg caaccggagc acctgcgtcg ctggttcggc cgtgacggct
     3841 ggtccttacc ggtctgcgag atcgacttgc ggcccggcgg cgcgtggcgg tacgtccacc
     3901 tggggccaga cggcatggaa ctcggactca gcggcgagta cctcgaggtc gacccgcccc
     3961 atcgactggt gtcgaccgag cgcttcgacg agccgtactt cgaggccatg ggcggcggca
     4021 cggtgaacac gatcaccttc gacgaggacg acggcgggat cacgacgatg acgctccgcg
     4081 tcttgtacgc ctcgaaagaa gcgcgcgacg gggcgctgca aaccggcatg gaggagggga
     4141 tggcggaagg gttccgccgc ctcgacgcgc atctcgcggg cctcgcgtag gccgctcgcg
     4201 aggaaggaga cacgatgcag aagattcacc cgatgctgtg gttcaacaca caggcggagg
     4261 aggcagcgga gttctacacc tcgctgttcc ccgactcgcg gatcacggat gtcgctcggt
     4321 tcggtgacgg cagcccgggg acggcgggca ccgtgatgac ggtgtcattt gagctcgcgg
     4381 ggcaacagct gacggcactc aacggcggac cggagttcaa cttcaccgag gcgatctcgc
     4441 tgtacgtcga ctgtgcagat caggcggaag tcgaccggct gtgggagcgc ctcaccgatg
     4501 gtggcgagcc gggccaatgt ggctggctga aggaccgctt cggcgtctcg tggcaggtca
     4561 ttccctcggc gctcggggag ctgatggggg accccgaccc tgagcgcacc ggccgcgtgg
     4621 tgcaggcgat gttgcagatg accaagatcg atg
//
LOCUS       DAORPG010000071         4629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1286_length_4629_cov_3.782903, whole genome shotgun sequence.
ACCESSION   DAORPG010000071 DAORPG010000000
VERSION     DAORPG010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1286_length_4629_cov_3.782903"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="PLX85_02545"
     CDS             complement(<1..1175)
                     /locus_tag="PLX85_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRC62086.1"
                     /translation="MSGRPTAGVEEEAFARGLGLGDFTLLVIGAIVGDGIYVLAAMGA
                     ASLGPAQLVAWLVAGVLAGFIILAFIQCAAIHPQVGGSYSYARHAFGPFVGFLAGWSL
                     LAGEWVALSAFPQAFFNYFHALTGAPESASLLVKLALVGLVTGVNLVGVRQSATTNDA
                     LTVAKLLPLGVLVVLAGLFALRHPSAASGHLHPFAPLGMGQLGSAVLPIFWAYAGFEL
                     AVLPAGEVRTPGRTLPRGLGIGLAIATGFYLLSTLAVVIAAPWEMTATSTHPVANAME
                     GVLAALGGPASAGFRFVSLGALVSIGGVFVVFMLGLSRLTYALAQDGLLPSMFGRLHG
                     RYRTPWANLLVQAVWALVFSTLFDLRSLLSTAVLFLSITYLLTALSAMRLVRRHPEQR
                     "
     gene            1283..2146
                     /locus_tag="PLX85_02550"
     CDS             1283..2146
                     /locus_tag="PLX85_02550"
                     /inference="COORDINATES: protein motif:HMM:NF015596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62087.1"
                     /translation="MPFSRLSAKRLWLPVLALALTALVVAACGGDDDEDSEYATSTAA
                     ASTATTAASATTPAASGSTVNIAVKTIDSGSYLAGPEGKALYVFTKDAAGKSNCTGQC
                     LQAWPPLTLKAGESVKADAAAKGKFDSIDTPSGKQVTYNGAPLYYYTPDVTASDVKGH
                     LVGNVWFLARPDTASNFVVSVRSDGSPKTPYLIGQTGMTLYTFANDTPNTSNCTGQCI
                     TNWPALLVADGQDPTAGPGAGGQLGVFVRDDGKRQVTYKGQPLYYWQGDKKPTDTTGD
                     GVGNVWKIAIP"
     gene            complement(2419..3498)
                     /locus_tag="PLX85_02555"
     CDS             complement(2419..3498)
                     /locus_tag="PLX85_02555"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HRC62088.1"
                     /translation="MINLELTSELDRSEVYEQLDVSGMRRLMAELPEQCRGAWEAGAR
                     WEIPATFFKPRRVVILGMGGSAIGADVAATLALRMGSVPLQIVRGYTLPHVDEETLVV
                     AASYSGDTEETLEAFQAALGTPGMRMALTSGGRLGRLAGQLGYAVLRYQYDGPPRAAI
                     GWGIFPLLAVLERLGALDVTAESVGTALRELAQASRDWGVETPYAENAAKQIAEALHG
                     RVPVIVGPDFFEVAARRWAGQMSENAKQWAFHGALPEVNHNLIVGFGKPGIAREALHV
                     LFLDSPLLHPRSRLRVRLTGEALDGAGIQHDELLIGGDEPLDAMLRGTYLGDWVSLYL
                     AMLNGVDPTPIEPIDQLKAKMARHG"
     gene            complement(3495..>4629)
                     /locus_tag="PLX85_02560"
     CDS             complement(3495..>4629)
                     /locus_tag="PLX85_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HRC62089.1"
                     /translation="ITASHNPARWNGFKIKQHYGGSAPPAVTKAIEESLDGIAAAGGV
                     QSMALGEAESRGLVERFDPRPTYLAALREFVDTQAIRQAGLNILVDSMFGAAMGYTME
                     LLAGDATEVHELHDARNPIFPGMRAPEPIAVNLAEQLGRIAQDGYDVGLSTDGDGDRL
                     GFADEHGRFITQLQTFALLAHYLVTGRGERGPIVRSVTMTRMVDRLGELYGCPVYETP
                     VGFVHLGPKMMENNALLAGEESGGYAFRGHIPERDGVLSGLLLLEAMVKTGKRPSELL
                     AALYAEVGPHEYDRVDITLRGDERDAIRARVAAAEPSTIAGLPVVSRDTVDGFRFVLE
                     GGWWLLMRFSGTEPLLRIYAEMPSSEQVQQALRAGQELAGISL"
BASE COUNT          740 a         1878 c         1365 g          646 t
ORIGIN      
        1 cgctgctccg gatgccgccg cacgaggcgc atggccgaca gtgcggtcag caggtaggta
       61 atgctgagga acagcaccgc ggtcgaaagc aggctccgca ggtcaaacag tgtgctgaac
      121 accagcgccc acaccgcctg caccagcaga ttggcccatg gtgtccggta ccgcccgtgc
      181 agccgcccga acatcgaggg caacaggccg tcctgcgcca gcgcgtacgt cagccgtgag
      241 agtccgagca tgaacaccac gaacaccccg ccgatcgaga ccagcgctcc gaggctcacg
      301 aagcgaaacc cggctgacgc cggtcccccg agcgctgcca gcaccccctc catcgcgttc
      361 gccaccgggt gtgtcgaggt cgccgtcatc tcccacggcg ccgcgatcac caccgccagt
      421 gtcgacagca ggtagaaccc cgtcgcgatc gccagcccga tgccgagccc tcgaggcagc
      481 gtccgccccg gcgttcgcac ctcgcccgcc ggcagcaccg ccagctcgaa ccccgcgtat
      541 gcccagaaga tcggcagcac cgccgatccc agttgcccca tccccagcgg cgcgaacgga
      601 tgcaggtgcc ccgaggccgc cgatggatgt cgcagcgcga agagtccggc cagcaccacg
      661 agcaccccga gcggcagcag cttcgccact gtcagcgcgt cgtttgtcgt cgcgctctgc
      721 cgcaccccga cgaggttgac ccccgtcacg aggcccacca gcgccagttt gacgagcagc
      781 gacgcgctct cgggcgcacc cgtcagcgcg tggaagtagt tgaagaacgc ctgcggaaac
      841 gccgacagcg ccacccactc ccccgccagc agcgaccacc ccgcgaggaa ccccacgaac
      901 ggtccaaagg catgccgcgc atagctgtag gacccaccga cctgcgggtg gatggccgcg
      961 cactggatga acgccaggat gatgaaccct gcgaggacgc ccgcaacgag ccaggccacc
     1021 agctgcgccg gcccgagcga cgccgccccc atcgccgcca gcacgtagat gccgtcgccg
     1081 acgatcgcgc cgatcaccag cagcgtgaag tcgcccagcc ccaggccgcg cgcaaacgcc
     1141 tcctcctcca cgcctgccgt cggtctcccg ctcatgccgg catcatccga catcgccggg
     1201 ccgcgggcct acctccgtca gtgatccgcg cgtcgtgccg gcgcgagtga tcttcgtgac
     1261 cgcttccgaa ggaggaatcc cgatgccctt ctcccgcctg tctgcgaaac ggctctggct
     1321 ccccgtgctc gccctcgccc tgacggccct cgtagtggcc gcctgcggcg gtgacgatga
     1381 cgaggacagc gagtacgcca cctcgaccgc cgccgcctcc accgccacga cggccgcctc
     1441 ggccaccacc ccggccgcgt ccggctccac ggtgaacatc gccgtgaaga ccatcgacag
     1501 cggctcgtac ctcgccggcc ccgagggcaa ggccctctac gtcttcacca aggacgccgc
     1561 aggcaagtcc aactgcaccg gtcagtgcct ccaggcctgg ccgccgctca ctctcaaggc
     1621 cggcgagagc gtgaaggccg acgccgccgc gaagggcaag ttcgactcca tcgacacgcc
     1681 cagcggcaag caggtcacct acaacggcgc cccgctgtac tactacaccc cggacgtcac
     1741 cgcctcggac gttaagggcc atctcgttgg caacgtctgg ttcctcgcga ggcccgacac
     1801 cgcctccaac ttcgtcgtca gcgtccgcag cgacggctcg ccgaagaccc cctacctcat
     1861 tggccagacc ggcatgacgc tctacacctt cgcgaacgac accccgaaca cgagcaactg
     1921 caccggccag tgcatcacca actggcccgc cctcctcgtc gccgatggtc aggacccgac
     1981 cgccggcccc ggcgctggcg gccagctcgg cgtcttcgtc cgcgacgatg gcaagcgcca
     2041 ggtcacctac aagggccagc cgctctacta ctggcagggc gacaagaagc cgaccgacac
     2101 caccggcgac ggtgtcggca acgtctggaa gatcgccatc ccctgacggg cgccaccgcc
     2161 cccgcgcgtc cccgcgcgag gcggcacgca caacgaaggg ccgggcagcc atgcccggcc
     2221 cttcccgatc cctctacagt cgttcaatgc tcaacgccgc tcgattcgat cgcgctgccg
     2281 ccgcgccccc cggccgcgcc aaccccgggg agcgccggga cttccagtcc gtcgcgttct
     2341 tgcccgtgcc ccgctgaagt cgagaggctc atctcgtagt tccgggcgtc agttccttcg
     2401 aggaggcccg cgcggtgtct atccgtgccg cgccatcttt gccttcagct ggtcgatcgg
     2461 ctcgatcggc gtcggatcca ccccgtttag catcgccagg tagagcgaca cccagtcacc
     2521 gaggtacgtc ccccgcagca tcgcgtccag cggctcatcg cccccgatca gcagctcatc
     2581 gtgctggatg ccggccccgt cgagcgcctc gcccgtcagc cgcacccgca accggctgcg
     2641 agggtgcagc aacgggctgt cgaggaacag gacgtgcagc gcctcgcgtg cgatccccgg
     2701 cttgccgaag cccacgatca ggttgtggtt gacctcgggc agcgcgccgt ggaacgccca
     2761 ctgcttcgcg ttctcgctca tctgcccggc ccaccgccgc gccgccacct cgaagaaatc
     2821 cggccccacg atcaccggca cacgcccgtg cagcgcctcc gcgatctgtt tcgccgcgtt
     2881 ctcggcgtac ggcgtctcca cgccccagtc ccgcgaggcc tgcgccagct cgcgcagcgc
     2941 cgtgccgaca ctctccgccg tcacgtccag cgctccgagt cgttcgagca cagccagcag
     3001 cgggaagatg ccccacccga tcgccgcccg tggcggcccg tcgtactggt agcgcagcac
     3061 ggcatacccg agctgccccg ccagccgccc gaggcggccc ccgctggtca gcgccattcg
     3121 catgcccggc gtccccagcg cggcctggaa cgcctccagc gtctcctccg tgtcgccgga
     3181 gtacgaggcg gcgacgacta gggtctcttc gtcgacgtgc ggcagcgtgt acccgcgcac
     3241 gatttgcagc ggcacggatc ccatgcgcag cgccagcgtc gccgccacgt cagcgccgat
     3301 cgccgacccg cccatcccga ggatcaccac ccgtcgcggc ttgaagaacg tcgccgggat
     3361 ctcccagcgc gccccggcct cccacgcgcc gcggcactgc tccggcagtt ccgccatcag
     3421 gcgtcgcatc cccgacacgt cgagctgctc gtacacctcg ctccggtcga gctcgctcgt
     3481 cagctcgagg ttgatcacag gctgatgccc gccagctcct ggcccgcccg cagcgcctgc
     3541 tgcacctgct ccgacgaggg catctccgcg tagatgcgca acaacggctc cgtcccgctg
     3601 aatcgcatca gcagccacca cccgccctcg agcacgaatc ggaacccgtc cacggtgtcg
     3661 cgcgacacca ccggcagccc cgcgatcgtg ctcggctccg cggccgccac ccgcgcccgg
     3721 atcgcgtcgc gctcgtcgcc gcgcagggtg atgtccactc ggtcgtactc gtgcggcccg
     3781 acctccgcgt acagcgcggc cagcaactcc gagggccgct tgcccgtctt caccatcgcc
     3841 tcgagcagca acaaccccga aagcacgccg tcacgttccg ggatgtgccc gcggaatgcg
     3901 tacccgcctg actcctcgcc cgcgagcagc gcgttgttct ccatcatctt cggacccagg
     3961 tgcacgaacc ccaccggtgt ctcgtacacc gggcagccgt acagctcgcc caggcggtcc
     4021 accatgcgcg tcatcgtcac cgaccgcacg atcggcccgc gctcgccccg gcccgtcacg
     4081 aggtagtgcg cgagcaacgc gaacgtctgc aactgcgtga tgaaccggcc gtgttcgtcc
     4141 gcaaacccca gccggtcgcc gtcgccgtcc gtcgacagcc ccacgtcgta cccgtcctgc
     4201 gcgatccgac ccagttgctc tgcgaggttc accgcgatcg gctcgggcgc tcgcatcccc
     4261 gggaagatcg ggttccgcgc gtcgtgcagc tcgtgcacct ccgtcgcgtc tcccgcgagc
     4321 agctccatcg tgtagcccat cgccgcgcca aacatcgagt ccacgaggat gttcaggccg
     4381 gcctggcgga tcgcctgcgt gtccacgaac tctcgcagcg ctgcgaggta cgtcggccgc
     4441 gggtcgaatc gctccaccag cccgcgcgac tccgcctccc ccagtgccat cgactgcacg
     4501 ccgccggccg cggcgatgcc gtccagcgac tcttcgatcg ccttcgtcac cgccggcggc
     4561 gcagaccccc cgtagtgctg cttgatcttg aacccgttcc agcgcgccgg gttgtggctc
     4621 gccgtgatc
//
LOCUS       DAORPG010000072         4617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1290_length_4617_cov_3.479833, whole genome shotgun sequence.
ACCESSION   DAORPG010000072 DAORPG010000000
VERSION     DAORPG010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1290_length_4617_cov_3.479833"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            306..1172
                     /gene="lepB"
                     /locus_tag="PLX85_02565"
     CDS             306..1172
                     /gene="lepB"
                     /locus_tag="PLX85_02565"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRC62090.1"
                     /translation="MESGDTPAESPSGAARAEASGGMTFSTLSRYPGSGAGEAALPAS
                     SVARTPWSPTQDGAAEHDAFGQEPPSEPPASSVWPWGMHGLIETIEVLALALLMFLLV
                     RSVAQNFVVDGGSMEPTFHNGEMLIVNKLAYKNFNLSWLPFTDKDDWRPFGAPQSGDV
                     VVFKFPQDQNRDFIKRVIAIPGQTVEVRGGKVYLDGIQQNEPYLDQAPTYDYGPVTVP
                     KGQLFVLGDNRNNSYDSHSWGLLDQSLLIGRAEFRYWPFGRIGRIDHGGLSAEAGVTI
                     TQGASSSSSTAR"
     gene            1151..1717
                     /gene="pyrE"
                     /locus_tag="PLX85_02570"
     CDS             1151..1717
                     /gene="pyrE"
                     /locus_tag="PLX85_02570"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRC62091.1"
                     /translation="MVVDSEVTAALEDAGAVLEGHFQLNSGRHSDRYLEKFNLLQWPR
                     YTEMVCAKIAEATRGLAPETVAGPTTGGIILAYEVARQLGLRGIFAERAAEGGRAFQR
                     DFRLRAGERVLVVDDVLTSGASVRDTLDAVAATGADCVGVAVLVDRSGGAVRFGELPF
                     FAATEFALQTYEPGDCPLCRDGVPLTVT"
     gene            complement(1660..2499)
                     /locus_tag="PLX85_02575"
     CDS             complement(1660..2499)
                     /locus_tag="PLX85_02575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HRC62092.1"
                     /translation="MARSDVRSGLFEDDAAIRRVHGEAVLLLGGGRALLMQIAHPLVA
                     RAVSEHSSYQHDRWGRLLRTVRPMYAIIFGTADQAEAAATRVNRLHESVRGDGYRAMD
                     PRLLAWVLATLIDTSLLTYERFVRSLAPAEVAAYYQDARAAGMLLGVPRNALPGDIAG
                     FRRYVDDQVASIEVTDAARAIARDLFEPLPGTGPAMVLMRQLTAALLPPRLREAYGLR
                     SGPAQRAALEVTGAINRAASAVLPPRLRRPPGFLLPPNGHTRGQVTVSGTPSRQSGQS
                     PGS"
     gene            complement(2504..2992)
                     /locus_tag="PLX85_02580"
     CDS             complement(2504..2992)
                     /locus_tag="PLX85_02580"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HRC62093.1"
                     /translation="MLKRWADAVRRNHALEHATVAVLLARHGPRRLAGRASVDGFLIA
                     SDVDSEELTSCAREALERLQAGQASLAVSPLCGTNIAVSGLMAAAAATAALAKGKERD
                     GRFANAFMAAMVAVVAAQPVGRLVQEHLTTRPDLDDVEVIDTKPVFGGLRKVRTRSVI
                     RA"
     gene            complement(3051..4382)
                     /locus_tag="PLX85_02585"
     CDS             complement(3051..4382)
                     /locus_tag="PLX85_02585"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62094.1"
                     /translation="MANRVLLLLGLIACCLALAGRAPFRATHAEACAFLRTPAAYESD
                     QLRANYATAMDAASVNGLFAGDPYFGLPAVKVGTRASRVDGAARVPGTLLRALGWVES
                     NTAMAARSVNFDSVGPALVSFDCGYGIMQVTSGMTSPLGTDNQPTANQARVATHYLYN
                     IARGATILADKWNQAPDSRPIAGTDTNSDPSIVENWYYAVWSYNGFTGPGATQSNHPL
                     DPRFSATRERWKCDGTQSRTKYPYQEVVWGCMASPPTRNGQQLWSPLAASLPNLSDPR
                     FSGPLQLSNFVAPFSSMDIPTAQPSHSQAAPTVPGDFVSRILGSPVLSVPNTSIQLGL
                     DGGAAAAKATVTVENHGSGILVWTATPSANWIVIDPPAGVALGPEVPCGAGCSRSATF
                     TVTLNSAALPGAMTAGHITISAANGNSPPVEVSINAEADFELPAPGTSKAY"
     gene            complement(4375..4551)
                     /locus_tag="PLX85_02590"
     CDS             complement(4375..4551)
                     /locus_tag="PLX85_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62095.1"
                     /translation="MPIDWSTDGQYLAVRSFDGMTTQDPGEESLVIVATSGGRMTVSA
                     ANEVLFLGWVASSG"
BASE COUNT          698 a         1561 c         1657 g          701 t
ORIGIN      
        1 ctgctcgtcg ccgttcacgt agacggcgta cggcggctgc gcgccgcgcg ggaggccgtg
       61 gccgatgtcg cggcgcagcg catcgagttg ctcggggcgg cgtttcgcgc ctgaactcag
      121 cgtccagaga cgctacacgg ggcgctgcta tactcgatcc aatgtcagac acaccaccgg
      181 ccgagacggg gtccggagcc gaccccgccg aggatccggc cactctctca ggtttctcag
      241 gcgtttcgag cgagccccac cccccggaat cagcctccac ggaacccacg ccgcagcccg
      301 cggccgtgga gagcggtgac acgccggcgg agtctccgag cggcgccgcc cgcgctgaag
      361 cctcgggcgg aatgacgttc tccacgctgt cgcgctaccc ggggagcggc gcgggtgagg
      421 cggcgctccc cgcctcgagc gtcgcccgga cgccctggtc gccgactcag gacggggccg
      481 ccgagcacga tgcattcggg caagagccgc cgtcagagcc gcctgcctcc tccgtctggc
      541 cgtggggcat gcacgggctg atcgagacca tcgaggtcct cgcgctcgcg ctcctgatgt
      601 tccttctcgt gcgctccgtg gcgcagaact tcgtggtcga tggcggctcg atggagccga
      661 ccttccacaa cggcgagatg ctcatcgtga acaagctcgc gtataagaat ttcaatctct
      721 cctggctccc gttcaccgac aaagacgact ggcgaccgtt tggcgcgccg cagtcgggcg
      781 acgtcgtggt cttcaagttc ccgcaggatc agaaccgcga cttcatcaag cgcgtgatcg
      841 cgatccccgg ccagacggtc gaggtgcgcg ggggcaaggt gtacctcgac gggattcagc
      901 agaacgagcc gtatctcgac caggcgccca cgtatgacta tggaccggtc acagtgccaa
      961 agggccagct cttcgtgctg ggagacaacc gcaacaactc gtacgattcg cactcgtggg
     1021 gcctgcttga ccagtccctg ctcatcggac gagcggagtt ccgctactgg ccgttcggcc
     1081 gcatcggccg gatcgaccac gggggcctga gcgccgaggc gggagtcacc atcacgcagg
     1141 gagcttcatc atcgtcgtcg acagcgaggt aacggccgcc ctcgaggacg ccggcgcggt
     1201 cctcgaaggc cacttccagc tcaactccgg acgccactcc gaccgctacc tggagaagtt
     1261 caatctcctc cagtggccgc ggtacacgga gatggtgtgc gccaagatcg ccgaagccac
     1321 ccggggactc gcgccggaga cggtggcggg gccaaccacg ggcggcatca tccttgcgta
     1381 tgaagtggcc cgccagctcg ggctgcgcgg catcttcgcc gagcgagccg ccgagggtgg
     1441 gcgcgccttc cagcgtgact tccggctgcg cgccggggag cgtgtgctcg tcgttgacga
     1501 cgtgctcacg agcggggctt ccgtgcgcga cacgctcgac gcggtggccg cgactggagc
     1561 ggactgcgtc ggcgtcgcgg tgttggtcga ccgctccggt ggagccgtcc ggttcggcga
     1621 gctgccgttc ttcgccgcga ccgagtttgc ccttcagacc tatgagccgg gcgactgtcc
     1681 gctctgccgc gacggcgtgc cgctgaccgt cacctgaccc ctcgtgtgtc cgttcggcgg
     1741 taggagaaac cccggcggac ggcgcaggcg gggtgggagc acggcgctcg cggcgcggtt
     1801 gatcgcgcct gtgacctcga gggccgctcg ctgcgcgggg cctgagcgga gcccgtacgc
     1861 ctcccgcagc cgtgggggga gcagcgccgc tgtgagctgg cgcatcagga ccatcgcggg
     1921 gcccgtcccg ggcagtggtt cgaacaggtc acgggcgatc gcccgcgccg cgtccgtcac
     1981 ctcgatgctc gccacctggt cgtccacgta acgccgaaag cccgcgatat cgccgggaag
     2041 ggcattgcga ggtacgccaa gcagcatgcc ggcggcccgg gcgtcctggt agtaggcagc
     2101 cacctcggcc ggcgccaggg aacggacgaa gcgctcgtac gtcaacagcg aggtatcgat
     2161 cagcgtggcg agcacccagg cgaggagccg cggatccatg gcgcggtacc cgtcgccgcg
     2221 cactgattcg tgcagacggt tgacgcgcgt ggccgccgcc tcggcctgat cggccgtacc
     2281 gaagataatc gcgtacatcg gacggacggt gcgcagcagg cggccccagc ggtcgtgctg
     2341 gtagctgctg tgctcgctca ccgcgcgggc cacgaggggg tgcgcgatct gcatgagcag
     2401 cgcccggccg ccgccgagga gcaacaccgc ttcaccgtga acccggcgga tggccgcgtc
     2461 gtcctcgaag agaccggaac gcacatctga acgcgccacg gtgtcaggct cgaatgactg
     2521 acctcgtgcg caccttgcgc aggccgccaa acaccggctt cgtgtcgatg acctcgacgt
     2581 cgtcgaggtc ggggcgggtg gtcaggtgtt cctgcacgag gcggcccacg ggttgcgcgg
     2641 cgaccacggc gaccatcgcg gccatgaacg cgttggcgaa ccgaccgtca cgctccttgc
     2701 ccttcgcgag cgcggcggtg gcggccgccg ccgccatcag ccccgagacc gcgatgttcg
     2761 tgccgcagag cggcgaaact gcgaggctcg cctgtcccgc ctgcagccgc tccagcgcct
     2821 cgcgagcaca cgaggtgagc tcctccgagt cgacgtcgct ggcgatcaag aagccgtcga
     2881 cgctcgcgcg gccggcgagc cgcctcgggc catggcgcgc gaggagcacc gcgactgtgg
     2941 cgtgctcgag tgcgtggttc cgacggaccg cgtccgccca gcgtttcaac atgacgcccc
     3001 cgcttcggtg tgcacgctca gtgtaggcct gccgcgagcg gcaggcggcg tcagtacgcc
     3061 ttgctcgtgc ccggggcggg caattcgaag tcggcctcag cgttgatgct cacctcgacg
     3121 ggcggcgagt tgccgttcgc cgcgctgatt gtgatgtgcc ccgcggtcat cgcccccggc
     3181 agcgcggccg agttgagcgt gaccgtgaag gtggcgctgc ggctgcaacc ggcgccgcag
     3241 ggcacctcgg ggccgagggc cacgccggcg ggcgggtcga tgacgatcca gttcgccgag
     3301 ggggtggccg tccagacgag gatcccgctg ccgtggtttt cgaccgtgac ggttgccttc
     3361 gcggccgcgg caccgccatc gaggccgagt tgaatcgagg tattcggcac cgagagcaca
     3421 ggcgagccaa ggatgcggct gacgaagtcg ccgggaacgg tgggcgccgc ctggctgtga
     3481 gacggctgcg cggtcgggat gtccatcgaa gagaacgggg cgacgaagtt gctcaactgg
     3541 agggggccgg agaagcgtgg atcggacaga ttcggcagtg aagcggcgag cgggctccac
     3601 agctgctgtc cgttgcgggt cggtggcgag gccatgcagc cccagacaac ctcctgatac
     3661 gggtacttgg tgcgcgactg cgtgccatcg cacttccacc gctcccgcgt ggcgctgaag
     3721 cgtgggtcga gcgggtgatt ggactgcgtc gcgcccgggc cggtgaagcc gttgtagctc
     3781 cacaccgcgt agtaccaatt ctcgacgatc gacgggtcgg agttcgtgtc ggtgccggcg
     3841 atcgggcggg agtcgggggc ctggttccac ttgtcggcga ggatcgtcgc gcctcgggcg
     3901 atgttgtaga ggtagtgcgt cgcgacccgg gcctggttcg ccgtcggctg gttgtcggtg
     3961 cccagcgggg aggtcatgcc gctggtcacc tgcatgatgc cgtagccgca gtcgaacgat
     4021 acgagggcgg gtccgacgga atcgaagttc accgaacgcg ccgccatcgc cgtgtttgac
     4081 tccacccagc cgagggctcg cagcagggtg ccgggcacgc gcgcggcgcc gtcgacgcgg
     4141 ctcgcgcggg tgccgacctt caccgccggc aggccgaaat atgggtcgcc cgcaaacagc
     4201 ccgttcaccg aggcggcgtc catcgcggtg gcgtaattgg cgcgcaactg gtcggactcg
     4261 taggccgcgg gcgtgcgcag gaacgcgcac gcctcggcgt gggtcgcgcg gaacggcgcc
     4321 cggcctgcca gcgcgaggca gcaggcgatg aggcccagga gtagcaggac gcggttagcc
     4381 actgctggcc acccaaccga ggaagagcac ctcgttggcc gcgctcacgg tcatacggcc
     4441 tccgctcgtg gcgacgatta cgagtgactc ctcgccgggg tcctgcgtgg tcatgccgtc
     4501 gaatgagcgc acggcgaggt actgcccgtc cgtgctccag tcgatcggca cgtcgtagcc
     4561 tcgcgccggc ggggcgagcg ccgagcggca atggacctac accccgaccg cgacggg
//
LOCUS       DAORPG010000073         4616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1291_length_4616_cov_3.192721, whole genome shotgun sequence.
ACCESSION   DAORPG010000073 DAORPG010000000
VERSION     DAORPG010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1291_length_4616_cov_3.192721"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1340
                     /locus_tag="PLX85_02595"
     CDS             <1..1340
                     /locus_tag="PLX85_02595"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC62096.1"
                     /translation="IRDVTAAATTTADEQQARVFYGWWIVAGAIVGYFVSVAAGAGSA
                     GIFLRPIVDDLGWSVGSFTLAITGTNAVSGLAGFFIGPLVDKHGARPLMFVGTGVLSG
                     SLLLISRVQAPWQFFLLQSVGVGIGISLVGGLVLNITMSKWFVLRRGWAIALGSTGIS
                     FASILIPLTLTRVVDNFGWRDGYVVLAIGAAILSVPVALVMRRQPEDYGLMPDGRRSD
                     GVGTSQADHDAITLDRLNSYTRREAVRTRALWLLTLSFGCFGGGAFGVLIHGIPFVTE
                     AGFTRSEAAFAASVAGMANLIAKFFWGYTLARFHVRTLWVGSFSGMIVGVGLMLLARE
                     TGQLPLMLFGFFVWGLGFGGGVPLGEFIWAKYFGRVHIGAVRSIGMPIGIAFGAAGPL
                     AVSTLFDATGAYTWAWLMLASVYVAGAVAVIVSREPPPKVTSLADLPLAAAEA"
     gene            1453..1866
                     /locus_tag="PLX85_02600"
     CDS             1453..1866
                     /locus_tag="PLX85_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62097.1"
                     /translation="MLGNKVWEGTGSTTGNRVLPGDDFRYVKLEISFEGMGKVLGTDA
                     SNMGTLTVFERVPGQMFAQGQGILMTADGQSAIWNGQGVGHPTGEGMGVSFRYGVAFQ
                     ADPNGSLAALNGSFFVGEYESAADGSWTDALWEWK"
     gene            1972..3537
                     /locus_tag="PLX85_02605"
     CDS             1972..3537
                     /locus_tag="PLX85_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC62098.1"
                     /translation="MTGHTPLFSQVSRLLNEADRRNREERGLSRRRFLKRGALSLGAA
                     ATVAALPSGVFAKSQPRITIVGAGIAGLSAANELQHAGLTATIYEGSNRIGGRMWSGH
                     DIMGPGLTTEIGGEFIDSGHREMLAYARAFNLPLIDVESPSEQALVKAFYFDGQVKTA
                     EQVIEAFRPVADVIARDQREVAFTDYTNYNQRALELDNTPLSDYLVQVGATGFLRELL
                     DVAYLTEYGGETNVQSALNLVFLIGTKTNQGFEEFGVSDQRYKVVGGNQRITDALASQ
                     VQQGGSPIRLGERLVAVREQRGGGRKTFVCTFASAGGTHEVTTDILLLTTPFSVLRDI
                     DLDVPLVPRVRHYIDECGYGTNSKLVLGFNRRYWRDSGFSGLFFTDLALQSGWDSSQL
                     QPGDLASLTIFTGGNQGIAAGAGSLQSQRNAALPRVEQIFPGANANKNDRAFRFIWPT
                     YEWSRGSYTSFTPGQYTAFTGSAQPTVIGDYPEPSGQLFFAGEHLNFDFQGYMEGGAV
                     SGKEAARQIARVA"
     gene            3855..>4616
                     /locus_tag="PLX85_02610"
     CDS             3855..>4616
                     /locus_tag="PLX85_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HRC62099.1"
                     /translation="MNEGIPLRVWPGRPFPLGASFDGAGTNFALFSEVAERVELCLVD
                     DQGRETRVDLPEVTSLCWHGYLPDVYPGQRYGFRVHGPWDPATGHRCNPAKLLLDPYA
                     RAIEGSIEWNPALYPYNLGAEDDSPNLEDSAPFVPKAVVANPFFDWGNDRPPSVPKHN
                     TVIYEVHVRGFTSQHPDLPEEIRGTYAALASPPVVAYLQQLGVTTVELLPVHEFVHDH
                     ALIERGLRNYWGYNSIGFFAPHHEYARGIAPGNQVL"
BASE COUNT          722 a         1744 c         1347 g          803 t
ORIGIN      
        1 gaatccgcga cgtgaccgca gctgccacca cgaccgcaga cgaacagcag gcccgcgtgt
       61 tctacggctg gtggatcgtc gccggcgcga tcgtcggcta cttcgtctcc gtcgccgcgg
      121 gcgccggctc cgccggcatc ttcctccgcc cgattgtcga cgacctcggt tggagcgttg
      181 gctccttcac cctcgccatc accggcacca acgcggtcag cggcctggcc ggcttcttca
      241 tcggtccact ggtcgataag catggcgccc gcccgttgat gttcgtcggt actggcgtcc
      301 tctcaggctc attgctcctc atctcgaggg tccaggcgcc ctggcagttc ttcctcctcc
      361 agtcagtcgg agtcgggatc ggcatctccc tcgtcggcgg cctcgtcctg aacatcacca
      421 tgtcgaagtg gttcgtcctc cgccgaggct gggccatcgc cctcggctcc accggcatct
      481 ccttcgccag catcctcatt ccgctcacgt tgacacgcgt cgtcgacaac ttcggctggc
      541 gcgatggcta tgtcgtcctc gcgatcggcg ccgccatcct ctccgttccc gtcgccctcg
      601 tcatgcgtcg ccagcccgag gactacggcc tcatgccgga tggccgccgc agcgacggcg
      661 tcggcacctc gcaggctgac cacgacgcca ttaccctcga ccgcctgaac tcctacaccc
      721 gccgcgaggc ggtgcgcacc cgcgcgctct ggctcctcac actctccttc ggctgcttcg
      781 gcggcggcgc cttcggcgtc ctcatccacg gcatcccctt cgtcaccgag gcaggcttca
      841 cccgcagcga ggccgccttc gccgcctccg tcgcgggcat ggccaacctc atcgccaagt
      901 tcttctgggg ctacaccctc gcgaggttcc acgtccgcac gctctgggtc ggtagcttca
      961 gcggcatgat cgtcggggtc ggcctcatgc tcctcgctcg cgagaccggc cagctcccac
     1021 tcatgttgtt tgggttcttc gtctggggcc ttggcttcgg cggaggcgtc cccctcggcg
     1081 agttcatctg ggcgaagtac ttcggtcgcg tccacatcgg cgccgtgcgc agcatcggca
     1141 tgcccatcgg catcgccttc ggcgcggccg gcccactcgc cgtctcgacg ctcttcgacg
     1201 cgaccggcgc ctacacctgg gcctggctca tgctcgcctc cgtctacgtc gccggcgccg
     1261 tcgccgtgat cgtttcccgc gagccgcccc caaaggtcac ctcgcttgcc gacctcccgc
     1321 tcgcggccgc cgaggcctga gcctcgcacc agacttcgtc agattcgtag cctctccctc
     1381 gatcggccgg ccgtatcatg cgcgcacgcg cgaggggttc tcgcgccaac aagtgcgcaa
     1441 aggagtggaa ccatgctagg caacaaggtg tgggaaggca cgggaagcac caccggcaac
     1501 cgcgtgctcc ccggtgacga ctttcgctac gtcaaactcg agatctcctt cgaggggatg
     1561 ggcaaggtgc tcgggacgga cgccagcaac atgggcaccc tgaccgtctt cgaacgtgtc
     1621 cccggtcaga tgttcgccca gggccagggc atcctcatga ccgcggatgg ccagagcgcg
     1681 atctggaacg gccagggcgt tggccacccg accggcgagg gcatgggcgt ctcgttccgc
     1741 tacggagtcg ccttccaggc ggacccgaac ggatcccttg ccgccctgaa cggctccttc
     1801 ttcgtcggtg agtacgagtc tgccgcggat ggcagctgga ccgacgccct ctgggagtgg
     1861 aagtaggtag gggccacgag agacctcgat cgcaggatcg aggtctctcc ttcaaccggg
     1921 accaaccgtt agtccctagg tcgaaccttc actacgtgtt atcaatgcac gatgacagga
     1981 cacacccccc tcttctccca ggtatcccgc cttctgaacg aggctgaccg ccgcaaccgt
     2041 gaggaacgag gcctcagtcg ccgccggttc ctcaagcgtg gcgcactcag cctcggagca
     2101 gccgccacgg tcgcggccct tccatccgga gtcttcgcca aatcccagcc gaggatcacc
     2161 atcgtcggtg ctggcatcgc cggcctgagc gcggccaacg aactgcagca cgccggcctt
     2221 acggcgacca tctacgaagg ttcgaaccgc atcggcggcc gcatgtggag cggtcacgac
     2281 atcatggggc cgggcctcac caccgagatc ggcggtgagt tcatcgactc cggccaccgg
     2341 gaaatgctcg cctacgctcg ggcgttcaat ctccccctga tcgacgtcga atcccccagc
     2401 gagcaggccc tggtcaaggc gttctacttc gacggccagg tcaagaccgc ggagcaggtc
     2461 atcgaggcct tccggccggt cgccgatgtg atcgcacgag accagcgcga ggtggccttc
     2521 accgactaca cgaactacaa ccagcgcgcc ctcgaactcg acaacacccc gctctccgat
     2581 tacctcgtgc aggttggggc caccggcttc ctgcgcgaac tgctcgacgt cgcctacctc
     2641 accgaatacg gcggggagac gaatgtgcag tccgccctca acctcgtctt cctgattggc
     2701 acgaagacga accagggctt cgaggagttc ggtgtctccg accagcgcta caaggtcgtc
     2761 ggcggcaacc agcggatcac ggacgccctc gcttcccagg tccaacaggg gggctccccg
     2821 atccgtctcg gcgagcgcct cgttgccgtc cgtgagcagc gtggtggcgg acgcaagacc
     2881 ttcgtctgca ccttcgcctc ggccggcggc acgcacgagg tcacgacgga catcctgctg
     2941 ctgaccacgc cgttcagcgt cctccgcgac atcgacctcg acgtccccct tgtcccgcgg
     3001 gtccgtcact acattgatga gtgcggttac ggcaccaact ccaaactcgt gctcggcttc
     3061 aaccggcgct actggcgcga ctccggcttc agcggcctgt tctttacgga cctcgccctg
     3121 cagtccggct gggatagttc gcaactgcag cccggcgacc tcgccagcct caccatcttc
     3181 accgggggca accagggtat cgccgcgggc gccggttccc tgcagtcgca gcgcaatgcc
     3241 gccctccccc gggtcgagca gatcttcccc ggcgccaacg cgaacaagaa cgaccgcgcc
     3301 ttccgcttca tctggcccac ctacgagtgg agccgcggca gttacacgtc gttcacgccc
     3361 ggtcagtaca ctgccttcac cggcagcgcc cagcctacgg tcatcggtga ctaccccgag
     3421 ccgagcggcc agctcttctt cgccggcgag cacctcaact tcgacttcca gggctacatg
     3481 gaaggcggcg ccgtctccgg caaggaagcc gcccgccaga tcgcgcgagt cgcctgagaa
     3541 ttctgcgtgt ggccggcgcg cacctcgcgc cggccacggt gggcggccca aacagcatgg
     3601 cgaacggggt ggagagcccg cgaggcgcct ccctctgccg ggccctccga ggcgcgagca
     3661 ccggaggcgc gtggcgctgt gctcacacgt aacgtaatag ctcagtcaag cattgcccca
     3721 gcgtaaggtt ccggatcggc ccggcgaaga cagcgggcgg cttcgccgcc gcattgcgcc
     3781 cgctcgtcgg gagcgggact ggtcgcctcc cctaccatcg aagtcagtcc cacataccct
     3841 gatgaggagg ctcagtgaac gaggggatac cgcttcgcgt ctggcccggc cggcccttcc
     3901 ccctcggcgc ctccttcgat ggcgccggca ccaacttcgc gcttttttcg gaggtcgccg
     3961 agcgcgtcga gctctgcctc gttgacgatc agggccgcga gacccgcgtc gatctccccg
     4021 aggtgaccag cctttgctgg cacggctacc tccctgacgt ctaccccggc cagcgctacg
     4081 gcttccgcgt tcacggcccg tgggacccgg ccactggtca ccgctgcaac cccgccaagc
     4141 tcctgctcga cccctacgcc cgcgccatcg agggctccat cgaatggaac ccagccctct
     4201 acccctacaa cctgggcgcc gaggacgact cgccgaacct cgaggacagc gcccccttcg
     4261 tcccgaaggc cgtcgtcgcc aaccccttct tcgactgggg caacgaccgc cccccctccg
     4321 tcccgaagca caacaccgtc atctacgaag tccacgtcag gggcttcacc agccagcatc
     4381 ccgatctccc cgaagagatc cgcggcacct atgccgcgct cgccagtccg ccggttgtcg
     4441 cctacctcca acagcttggt gtgacgaccg tcgagttgct ccctgtccac gagttcgtgc
     4501 acgaccacgc cctgatcgag cgtggactcc gcaactactg gggctacaac tccatcggct
     4561 tcttcgcacc gcaccacgag tacgcccgcg gcatcgcgcc cggcaaccag gtcctc
//
LOCUS       DAORPG010000074         4602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1296_length_4602_cov_3.391907, whole genome shotgun sequence.
ACCESSION   DAORPG010000074 DAORPG010000000
VERSION     DAORPG010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1296_length_4602_cov_3.391907"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PLX85_02615"
     CDS             <1..634
                     /locus_tag="PLX85_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HRC62100.1"
                     /translation="AASWVSTSPSSFGPLPLPASVTSTVNVNVPSGAAAGTYTYDLVA
                     LADGLDVGHVALTLNVTAGGGGGVPGGTPTPDNQKRNRGGATTSGPDVQITGSASTGS
                     PAPGAQYRYTYQVKNNSGSTATSVLLEDSLPTDVTVVGVTTTGGVCSAAGVQVACNLG
                     SLAVGGQQTVVVTVQAPAAPGPVTNTATVSLAEVDTKPANNSLTIAVTVK"
     gene            complement(853..1389)
                     /locus_tag="PLX85_02620"
     CDS             complement(853..1389)
                     /locus_tag="PLX85_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRC62101.1"
                     /translation="MDEARAQLLERVIAEVSANGLADRSLREIAAAVGSSHRMLHYHF
                     GSRAGLVAAVVEAVEGAQRELLVELSREITDPVELSRELWLRTSTPELLPFVRLFFEC
                     VGLTGGQGLTDPWLMLGQEIGERIGVAVDMDEFRLGVAVTRGLLIDVLAAGDATEATR
                     SFERFLELWAASRIDAEA"
     gene            1465..1881
                     /locus_tag="PLX85_02625"
     CDS             1465..1881
                     /locus_tag="PLX85_02625"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRC62102.1"
                     /translation="MRIEHTIEIAAPADRVWELTLDVEKWPELTPTITSVKRLDGGPM
                     AVGSQARIKQPAQGTRVWTVTDLEPKRHFVWSTKVMGSRMTGGHHLSQGATGTTNRLT
                     VDIEGPFAPVLGVLLRRPILNAITKENQGFKRAAEA"
     gene            complement(2016..2156)
                     /locus_tag="PLX85_02630"
     CDS             complement(2016..2156)
                     /locus_tag="PLX85_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62103.1"
                     /translation="MRLFGKKQHSAAVQAPLCLHLAMTARWDSVADMGDESNVTSFVR
                     AT"
     gene            2516..4249
                     /locus_tag="PLX85_02635"
     CDS             2516..4249
                     /locus_tag="PLX85_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC62104.1"
                     /translation="MTDNQLISRKTLLRTGALGGAGLLAAALLGCGSDDEGSPAASSS
                     ATAAATAEPGVIRDPNLPYPFQAPDPAGAPKTGGTMRYGTTYAISIFDPAKTSAGGTL
                     MVTNFVYNRLLGFKRGVDMDPFRLDLIPELASSWERTPDGLTYTFKLVSNAKFHNVAP
                     VNGRTLVADDVKFSYDRYAAGGPQQSYFANVASVDAVNPTTLKITMKRPVADFIMPLA
                     GRYIPIIPKELVDDGSIEKKAIGTGPMLLKEAIDGQRAYFERNPEYFQRPVLLDAFEY
                     KIIPAPAARLAAFRSRQIDYGYPFVDNITDAKNLEKTTPDVRFFMSAPLNTTFTVAFN
                     LDLPKYKDDRVRQGLALAVDHNTIINVVLDKLGLALPAIPWPFVFDKLPTGDQLGRFM
                     KFDPAEGKRLLDAAGAAGLEINSNYYQYTSTNSRVSDLLVDQLRAVGVTLKNQQADYT
                     EFNSQWVGAKIADATTSGWGTAGFDADNWFYNQIHSKSPGNRWRIKDAQSDTWAEQQQ
                     VELDPAKRREIHRKLWDYHNEKQYRLSTPVGYGFTPLQPWVRGVRGGNWGNSLSYDDG
                     AIVGAGAWLDK"
     gene            complement(4374..>4602)
                     /locus_tag="PLX85_02640"
     CDS             complement(4374..>4602)
                     /locus_tag="PLX85_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62105.1"
                     /translation="DVTLVGATQIGPSFGAGGGSFTASGVAPYRGFSTSWAFEGNVIA
                     GRGLSGTLTIGGDGRLPGGRAIAYRLDGTRR"
BASE COUNT          804 a         1819 c         1311 g          668 t
ORIGIN      
        1 cgctgcttcg tgggtctcga cctcaccgtc gtcgttcggc ccgctcccgc ttccggcttc
       61 ggtcacctcg acggtgaatg tgaacgtccc gtccggagcg gcggccggca cctacaccta
      121 cgacctcgtc gcactcgcgg acggcctcga cgtcggacac gtcgcgctca cgctgaacgt
      181 cacggctggt ggtggcggtg gagtccccgg cggcacgccc acgccggaca accagaagcg
      241 gaaccggggt ggcgcgacca ccagtggccc tgacgtccag atcaccggct ccgccagcac
      301 cggctctccg gccccgggtg cacagtaccg ctacacctac caggtcaaga acaacagcgg
      361 atcgacggcc acctcggtcc tgctcgagga cagcctgccc accgacgtca ccgtggtcgg
      421 cgtgacgacg accggcggcg tctgctcggc ggccggcgtg caggtcgcct gtaacctcgg
      481 ctccctcgcg gtcggcggac agcaaaccgt cgtcgtcacg gtgcaggccc cggctgctcc
      541 cggtccggtg actaacaccg ccaccgtctc gctggccgag gtcgacacca agcctgcgaa
      601 caactcgctc accatcgcgg tgacggtgaa gtaagtccgc caacggacac cgagaggggg
      661 acgccctatt gggcgtccct cctcccactc tcgccctgga tccctccggc gcctgtcccc
      721 gtatctcggc cgacgaccgc gcctggctcc ggtcggacgc cccgatcctc gccaacgagc
      781 ccgtgcgccg gtgccgcccg gtccgaggtc acacaacttc cagaaaagtg ccccgaggga
      841 cacggggcgg cgctacgcct ccgcgtcgat ccgcgatgcc gcccacaatt cgaggaaccg
      901 ctcaaacgac cgcgtcgcct cagttgcgtc gccagccgcc agtacgtcga tcagcagtcc
      961 ccgcgtcacc gccacaccga ggcgaaactc gtccatgtcg accgccacgc cgattcgttc
     1021 gccaatctcc tgtcccagca tcaaccacgg gtcggtgagc ccctgcccgc ccgtcagccc
     1081 cacgcactcg aaaaacagcc gcacgaaggg caagagctcg ggcgtcgagg tccgtagcca
     1141 caactcgcgt gacaactcca ccgggtcagt gatctcccgc gacagctcga ccaacaactc
     1201 gcgttgcgcc ccctccaccg cctcgaccac cgccgcgacg aggcccgcac gcgacccgaa
     1261 atggtagtgc agcatccggt gactcgagcc cacagccgcg gcaatctctc gcaggctgcg
     1321 gtccgccagc ccgttagcgc tcacctccgc gatcacccgc tccagcagtt gtgcccgcgc
     1381 ctcatccatc agggcctccc ttgcgcgctg agtgtaccat ttggtacagt cgtcgcacac
     1441 ccgccacccg agaggaggaa cgccatgagg atcgaacaca caatcgaaat cgccgcgcct
     1501 gccgaccgcg tctgggagct caccctcgat gtcgagaagt ggcccgagct gacgcccacc
     1561 atcaccagtg tcaaacgcct cgacggcggc ccgatggccg tcggctcaca ggcccgcatc
     1621 aagcagccgg cccagggcac ccgcgtctgg accgtcaccg acctcgagcc aaaacgccac
     1681 tttgtctggt ccacgaaggt catgggcagc cgcatgaccg gcggccacca cctgtcccag
     1741 ggcgccacgg gcacgacgaa ccgcctgacg gtggacatcg agggcccgtt cgcccccgtc
     1801 ctcggcgtcc tcctacgtcg acccatcctg aacgccatca cgaaggagaa ccagggcttc
     1861 aagcgcgccg ccgaggcctg atcgccgtcg gcctgcagct gcagcgtgaa agggccgcga
     1921 gtcgcggccc cttcacgcta ggcgaggtcg cgtcgaatct gttcggcctg cgcgtcccgg
     1981 attcgagcgg cctccgctgg agcgaactcc gcgcctcacg tcgcgcgcac gaacgaagtc
     2041 acgttcgact cgtcgcccat gtccgccacg ctatcccagc gcgccgtcat cgcgaggtgc
     2101 aagcacagtg gagcctgcac cgccgccgaa tgctgcttct tgccaaagag tcgcatcgag
     2161 gtgctcctct tgccgcccct cggcgatcgt ccgtgcgaga ggttgcacgc gctcaacagg
     2221 agcgcctcgc atatgccggt gccacgcgaa cgcgctaaga ctgagcgaac cgccagtccg
     2281 ccatgacctg cggacatgcg gggagacgtg ggcgaggccc gttcgtcgtc tccgtggccc
     2341 gcgcgatcca gcttccttgc cggacacccg gctccccccg acgagtgatc ggctccgcgg
     2401 caccggcgct cagtcggtcg ggcgcccagg gggcctggta aagcattgcc ccacttaatc
     2461 gttcgtaata ggcttggccc gcccccacga tgaccgaacg acgaaggtgg taaacgtgac
     2521 cgacaaccag ctcatcagcc gcaagacgct gctgcgcacc ggcgccctcg gaggagcagg
     2581 cctcctcgcc gctgcgctgc tcggctgcgg cagtgatgac gagggctccc ccgccgcctc
     2641 gagcagcgcg accgccgcgg ccaccgcgga gccgggagtc atccgcgacc ccaacctccc
     2701 ctaccccttc caggcccccg acccggccgg cgcgcccaag accggcggca cgatgcgcta
     2761 tggaacgacc tacgccatct cgatcttcga ccctgcgaag acgtccgccg gcggcaccct
     2821 gatggtcacc aacttcgtct acaaccgcct ccttggcttc aagcgcggcg tcgatatgga
     2881 cccctttcgt ctcgacctca tcccagagct cgcctcgagc tgggaacgca ccccggacgg
     2941 actcacctac accttcaagc tcgtctcgaa cgcgaagttc cataacgttg cgcccgtcaa
     3001 cggccgcacc ctcgtcgccg acgacgtcaa gttctcctat gaccgctacg ccgcgggcgg
     3061 cccccagcag tcctacttcg cgaacgtcgc ctcggtcgac gccgtgaacc ccaccaccct
     3121 caagatcacg atgaagcgcc ccgtcgccga cttcatcatg cccctcgcag gacgctacat
     3181 ccccatcatc ccgaaggagc tcgtcgacga cggcagcatc gagaagaagg ccatcggcac
     3241 cggcccgatg ctcctcaagg aagccatcga cggccagcgc gcctacttcg agcgcaatcc
     3301 cgagtacttc cagcgccccg tcctgctcga cgccttcgag tacaagatca tccccgcccc
     3361 cgcggcgcgc ctcgccgcct tccgctcccg ccagatcgac tacggctacc ccttcgtcga
     3421 caacatcacc gatgcgaaga acctggagaa gacgacgccc gacgtccgct tcttcatgtc
     3481 cgctcccctg aacaccacct tcacggtcgc cttcaacctc gacctcccga agtacaagga
     3541 cgaccgcgtc cgccagggcc tcgccctcgc cgtcgaccac aacacgatca tcaacgtcgt
     3601 gctcgacaag ctcggcctcg ccctccctgc gatcccctgg cccttcgtct tcgacaagct
     3661 ccccaccggc gaccagctcg gccgcttcat gaagttcgac cctgccgagg gcaagcggct
     3721 cctcgacgcc gctggtgcgg ccggcctcga gatcaactcc aactactacc agtacacgtc
     3781 gaccaacagc cgcgtctccg acctcctggt cgaccagctg cgagccgtcg gcgtcaccct
     3841 gaagaaccag caggccgact acaccgagtt caactcgcag tgggtcggcg ccaagatcgc
     3901 ggacgccacc accagcggct ggggtaccgc cggcttcgac gccgacaact ggttctacaa
     3961 ccagatccac tcgaagtcgc cgggcaaccg ctggcgcatc aaggacgccc agagcgacac
     4021 ctgggcggag cagcagcagg tcgagctcga ccccgcgaag cgccgcgaga tccaccgcaa
     4081 gctctgggac taccacaacg agaagcagta ccgcctctcg acccccgtcg gctacggctt
     4141 caccccgctg cagccctggg tccgaggcgt gcgcggcggc aactggggca actcgctctc
     4201 ctacgacgac ggtgccatcg tcggcgctgg cgcctggctc gataagtagc ccctcacgcc
     4261 gctgtccgtc accagcctgg agcccgccat acggcgggct ccgtcgcctg cggtgagcgc
     4321 tcgccatcac cgggctgcca tcgaacgcga gagacgttcg gaggcgcttg cccctatcgc
     4381 ctggtcccgt cgaggcgata cgcgatcgcc cggccgccgg gcaatcgccc gtcgccgcca
     4441 atagtgagcg tgccgctcag gccgcgtcct gcgatcacgt tcccctcgaa cgcccagctc
     4501 gtcgagaacc cccggtacgg cgcgacgccc gacgccgtaa acgagccgcc tcccgccccg
     4561 aacgatggcc cgatttgagt cgcgcccacg agcgtcacgt cc
//
LOCUS       DAORPG010000075         4583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1310_length_4583_cov_2.625442, whole genome shotgun sequence.
ACCESSION   DAORPG010000075 DAORPG010000000
VERSION     DAORPG010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1310_length_4583_cov_2.625442"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..>4583
                     /locus_tag="PLX85_02645"
     CDS             15..>4583
                     /locus_tag="PLX85_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRC62106.1"
                     /translation="MPEGVRFDAAWLPDNVTTVVLPAGDDLGGWAGLRRVAQVTSIEE
                     ARAAQAAGADGLIAKGSESGGRIGDETAFVLLQSLVQELSLPIWVQGGIGEYTAAACV
                     AAGAAGVVLDSQLALLEECVLPDEVRRAVAAMDGSETVVVHGHRVYSRPGLHVTELGA
                     DELIQRLGGGSLGASVLPAGADAALAAGLARRYGSTARLITGLERRIDAHLALAARAS
                     VLDETSAFARRHGTGAAIAQGPMTRVSDHPDFAIAVSEAGGLPFLALAVMSGADSRRL
                     LTEAASKLAGKTWGVGILGFISPELQEQQLSAIEEVRPPVALIAGGRPEQAAKLEALG
                     TATYLHVPSPGLLDLFLRQGARRFVFEGQECGGHIGPRSSFVLWEQQLVRLMEFEHPE
                     ELSVLFAGGIHDARSAAAVSVLAAPLAAKGASVGVLMGTAYLFTNELVSTGALQPLFQ
                     QTAIDCDRTVLLETAPGHVTRCADTEYVRAFEAERERLVAAGLPKDEVWSALEDLNLG
                     RLRIASKGIVRQGSELVTVDEATQRREGMVMLGQVAALERGRQSIRELHADVAERSFD
                     LVRDAAGRSTDSGRPRDEDIAIVGMSVILPGASDVPTYWSNIVNGVNSVTEVPPDRWD
                     PAIFYDSDSKTGEMTPSKWGGFIEPVEFDPLAYGIPPRSLAAIDPAQLLAPEASQRAL
                     RDAGYDQRAFNRELASVIFGTDGGSELSAGYSFRPHWRQYVGEIPAELDEVLPSLTED
                     SFPGILGNVVAGRIANRLDLGGVNYTVDAACASSLAAVDLALKELRGGTSDLVICGAV
                     DFHSGIVDYLSFSSVHALSPSGQCKTFDATADGIALGEGVAAVILKRLSDAERDGDRI
                     YAVIKGVAGGSDGKSLGLTAPRQEGQVRTLRRVYERSGVSPAAVGLVEAHGTGTVVGD
                     RTELETLNEVFRHAGAAPESCALGSVKSQIGHTKCAAGLAGIIKAALALHYQVLPPTL
                     NVETPNPGWDPTSPFAINTEPRPWPGPERFAGVSAFGFGGTNFHAVLGSYDASGTPES
                     SPGWPAELFLIRGVDQADARRTAETLRAAVSGQDGWRLADLARTVSTMPGAVQFAFVA
                     ADLDELRAKLADFGAGVFERPEQPLGVASEVAFLFPGQGSQKPGMLADLFVHFPSLQR
                     YLELGERWRHIMIPPPAWSPQDAAMQKQVLTDTRNAQPALGIAGLATYDLLSGLGIEP
                     SMLGGHSYGELVALCAAGAFSPRDLLALSEARAAAIAECIPDGGDAGSMAAAMSDLET
                     LRGLLDGMPGVVIANQNAPDQNVLSGATDAVNAARKRLREAGVRTVELPVSRAFHSPI
                     VAAAATRFEQVVAPVRMETPRRTVYSNTTAEPYPEEPTAIKQRLVEHIPAPVRFMHEI
                     LAMYEAGARIFVEAGPGQTLTGLVGRILGERPHVAIPVDQGSSTLTSFLGALAQLAVH
                     GVEFEVERLFEGRDAAVVDLAQPPAGPKPTSWMVNGHLARPVTGDAPAHGMKPVTRPI
                     AGLAATTRSEGAAAPLPEAPAGLPQRTAP"
BASE COUNT          711 a         1486 c         1686 g          700 t
ORIGIN      
        1 aattcggggt gcgtgtgccc gagggcgttc gcttcgacgc ggcatggctc cccgacaacg
       61 tcacgacggt cgtactgcct gccggagacg acctcggagg atgggcaggc ctgcggcggg
      121 tcgcccaggt cacctcgatc gaggaggcgc gagcggccca ggctgcgggt gcagacggcc
      181 tcattgcgaa ggggagcgaa tccggcgggc gcattgggga tgagacggcg tttgtgctgc
      241 tgcagtcact cgtgcaggag ctgtcgctgc cgatctgggt gcagggcggc atcggcgagt
      301 acacggcagc ggcctgtgtc gcagccggcg ccgccggggt cgtcctcgat tcgcagctgg
      361 cgctgctcga ggagtgcgtg ttgccggacg aggtacggcg cgccgttgcg gccatggacg
      421 gcagcgagac ggtcgtcgtc cacggccatc gggtgtacag ccgtccgggc ctccacgtca
      481 cggagctggg cgcagacgaa ctgatccaac gcctcggtgg cggaagcctt ggagcaagcg
      541 tgctgccggc gggcgcggac gcggcgttgg ccgcagggct ggcccgtcgc tacggaagca
      601 cggctcggct gatcaccggc ctcgaacggc gcatcgatgc acacctggcg ctggccgcgc
      661 gggcttcagt gctcgacgag acctcggcgt tcgcgcgacg gcacgggacc ggagccgcga
      721 tcgcacaggg tccgatgacg cgcgtgagcg accacccgga ctttgccatc gcggtgagcg
      781 aagccggcgg gctcccgttc ctcgcgctgg cggtgatgtc cggggcggat tcgcgacgtc
      841 ttctcactga agcggcctcc aagctcgcag ggaagacgtg gggggttggc atcctcgggt
      901 tcatctcgcc ggagctacag gagcagcagc tgagcgcgat cgaggaggtg cgaccgccgg
      961 ttgcactgat cgcggggggg cgcccggagc aggcggcgaa gctggaagcg ctgggcaccg
     1021 cgacgtactt acatgtgccg tcgcccgggc tgctcgacct ctttctccgg cagggcgctc
     1081 gacggttcgt attcgagggg caggaatgcg ggggtcatat cggcccccgc agcagcttcg
     1141 tgctctggga gcagcaactg gtccgactga tggagttcga gcatcccgag gagctctcgg
     1201 tcctgtttgc gggaggcatc cacgacgccc gctcggcagc ggccgtctcc gtgcttgcgg
     1261 cgccgctggc ggcgaagggc gcgagtgtcg gggtgctgat ggggacggcg tacctcttca
     1321 ctaacgaact ggtctcgacg ggcgcgctgc aaccactgtt ccagcagacc gcgattgact
     1381 gcgaccgaac ggtgctcctc gagacggcgc cggggcacgt gacgcgctgc gcggacacgg
     1441 aatatgtgcg cgcgttcgag gctgagcgcg aacggctggt cgccgccggc ctgccaaagg
     1501 atgaggtctg gagcgcgctc gaggatctga atctcggccg gctccgtatc gcctcaaagg
     1561 ggatcgtgcg tcaggggtcg gaactggtca cggtggatga ggcaacgcaa cggcgcgaag
     1621 gcatggtgat gctcggccag gtcgccgccc tcgagcgcgg cagacagtcg attcgcgaac
     1681 tgcacgccga cgtggcggaa cggtcgtttg acctcgtgcg cgacgccgct ggccggtcga
     1741 cggacagcgg caggccgcgt gacgaggaca tcgcgatcgt cggcatgtcg gtgatcctgc
     1801 cgggcgcctc cgatgttccg acgtactggt cgaacatcgt caacggggtg aacagcgtga
     1861 cggaggtccc gccggaccgc tgggacccgg cgatcttcta cgattccgat tcgaagaccg
     1921 gagagatgac gccctcgaag tggggcgggt tcatcgagcc ggtggaattc gacccgctcg
     1981 cgtatgggat cccaccgcgg tcactggcgg ccatcgaccc ggcccaactg ctcgcccccg
     2041 aggcgagcca gcgagcgctg cgggatgcgg gctacgacca gcgagccttc aaccgcgagc
     2101 ttgcgtcggt catcttcggc acggacggcg gcagcgagct ctccgccggg tacagcttcc
     2161 gtccgcactg gcgccagtac gtgggcgaga taccggcgga actcgacgag gtgctgccca
     2221 gtctcacgga ggactcgttc cccggcatcc tcgggaacgt ggtggccggg cggatcgcaa
     2281 accggctgga cctcggcggg gtgaactaca ccgtggacgc cgcgtgtgcg tcttcgctgg
     2341 ccgcggtcga ccttgcgctc aaggagctgc gcggcgggac gagtgacctc gtgatctgcg
     2401 gcgcggtgga cttccacagc ggaatcgtcg actacctctc cttctcgagc gtgcacgcac
     2461 tctcgccgag cgggcagtgc aagacgtttg atgccacggc tgacgggatc gcactgggcg
     2521 aaggtgtcgc ggcggtcatc ctcaagcgcc tgtcggacgc tgagcgtgat ggcgaccgga
     2581 tctatgccgt gatcaagggc gtggcgggag gcagcgacgg gaagagcctc gggctgacgg
     2641 cgccccggca agagggccag gtgcggacgc tgcggcgcgt gtacgagcgg tcaggcgtgt
     2701 cgccggctgc ggtcgggctg gtcgaggcgc acggcacggg caccgtggtt ggggaccgta
     2761 ccgagctcga aaccctgaac gaagtcttcc ggcacgcggg tgcggccccg gagagctgcg
     2821 cgctcggctc agtgaagtca cagatcggcc acaccaagtg cgcagcggga ctcgcgggca
     2881 tcatcaaggc agcgctcgcg ctgcattacc aggtgttgcc tccgacattg aacgtcgaga
     2941 cgccgaaccc gggttgggac cccacgagcc cattcgccat caacacagag ccgcggccat
     3001 ggcctggccc ggagcggttt gcgggcgtga gcgccttcgg gttcggcggg acgaacttcc
     3061 acgcggtcct cggcagctac gacgccagcg gtacccccga aagctcgccc ggctggccag
     3121 cggaactgtt cctgatccga ggagtggacc aagcagacgc acgccgcacc gccgagaccc
     3181 tgcgagccgc ggtctcggga caggatggct ggcggctggc ggaccttgcc aggaccgtct
     3241 caacgatgcc gggggccgtg cagttcgcgt tcgtcgccgc tgacctcgac gagctgcggg
     3301 ccaaattggc cgacttcggc gcgggggtct tcgagcgacc agagcagccg ctcggggtcg
     3361 cgtcagaggt ggcgttcctg ttcccgggcc agggaagcca gaagccggga atgctggcgg
     3421 acctcttcgt ccatttcccc tcgctgcaac gctacctgga gctcggggag cgctggcgtc
     3481 acatcatgat cccgccaccg gcatggtcgc cgcaggatgc ggcgatgcag aagcaggtgc
     3541 tcacggatac gcgcaacgcg cagcccgcac tcggcatcgc cggactcgcg acgtatgacc
     3601 tgctctcggg cctcggcatc gagccgtcga tgctcggagg gcacagctat ggcgaactgg
     3661 tcgccctgtg cgcggcgggc gcgttctcgc cgcgggacct gctggcgctc agcgaggctc
     3721 gtgcggcggc catcgccgag tgcattcctg acggcggaga cgccggcagc atggcggccg
     3781 ccatgtctga cctcgaaacg ctgcgagggc tcctcgacgg catgccgggt gtggtgatcg
     3841 cgaaccagaa cgcgcccgac cagaacgtgc tctccggggc caccgacgcg gtcaacgccg
     3901 ctcggaagcg gcttcgcgag gccggcgtgc ggacggttga actgccggtc agcagggcct
     3961 tccatagccc gatcgtggcg gcggcggcca cgcgcttcga gcaggtggtc gcgcccgtgc
     4021 ggatggagac gccacgccgc accgtgtatt caaacacgac cgccgagccg tatccggagg
     4081 aaccgacggc catcaagcaa cggctggtcg agcacatccc ggcgcctgtg cgcttcatgc
     4141 acgagatcct cgcgatgtac gaggcggggg cgcgcatctt cgtcgaggcg ggcccgggcc
     4201 agacgctgac cgggctcgta ggacgcattc tgggcgagcg tccgcatgtc gcgattcccg
     4261 tcgaccaggg tagttcgacg ctgacttcct tcctcggcgc gctggcgcaa ctcgccgtgc
     4321 atggggttga gttcgaggtc gagcggctgt ttgaaggtcg ggacgcggcc gtcgtggacc
     4381 tggcacaacc gccggcggga ccgaagccga cttcgtggat ggtgaatggc cacctcgcgc
     4441 ggccagtcac gggtgatgcg ccagctcacg ggatgaagcc agtcacccgc ccgatcgcag
     4501 gcctggccgc gaccactcga tccgaaggcg cggcggcgcc gctgcccgaa gcgccggccg
     4561 gcttgccgca acgcacggcg ccg
//
LOCUS       DAORPG010000076         4578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1313_length_4578_cov_3.242759, whole genome shotgun sequence.
ACCESSION   DAORPG010000076 DAORPG010000000
VERSION     DAORPG010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1313_length_4578_cov_3.242759"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..469)
                     /locus_tag="PLX85_02650"
     CDS             complement(80..469)
                     /locus_tag="PLX85_02650"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HRC62107.1"
                     /translation="MRRVMLMVALASAMAFLGTGAVRTEAAPPFHCNQVNMHPNGLPN
                     GQVGFAYSSTLSLTPASNLLPASVTNAPLLPPGLSFTPGPSSNQVTLSGTATVAGTYT
                     FTVELGATYVYGTICKVSQTYTVTIAP"
     gene            complement(542..1474)
                     /locus_tag="PLX85_02655"
     CDS             complement(542..1474)
                     /locus_tag="PLX85_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRC62108.1"
                     /translation="MSARPAPLERRPVTAGAALFTLGLAVLFGANPTAIKFGLRYLHP
                     LQIGSARFLLAGCALLAWALVSRRRVLPARSEVLPLVLLGALFVATIATTNVGQDHTS
                     VAHTVVILTTFPIWTAILSHLFVSGDRIGRAQVVGIVISYAGVIVTFGPGLAKGGSQL
                     HGDLLIFVSSFLLGGSQVLISILSQRIELAKIVLTQAFTAGILLFLVSVPIEGTDYEF
                     AWPLVWSLLYQGVLIGGFAFIANAWLLKTYPPSRISVIYSSQPLFGILSSWVVLGEDL
                     HSEVIFGVLLVSLGILIVQEVWSVFLRDRGRVTA"
     gene            complement(1484..2491)
                     /locus_tag="PLX85_02660"
     CDS             complement(1484..2491)
                     /locus_tag="PLX85_02660"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HRC62109.1"
                     /translation="MRDLIAAGPVRLAPMASYTNAPYRLVARECGAGFSTNEEIDAAA
                     LLGMGMSKAAVWTAIDPGEGPVAMQLLGNSEETLVPAAVRLVEAGASVIDINMGCPAK
                     KAVTKGKGAALMRDVPRTARILGALRRELTALDIPLTIKIRGGWDDTCLNAVEVARMA
                     EDVGVDAITVHPRTRSQQYAGRAPWEVIADVVRAVRIPVTGNGDVRSLAEAQRMMAAT
                     GCLGVMVGRGALGRPWMFDPEHEARPVGERRAHEAAARRRHLSLIEAMLPPREALVHT
                     RRQLSLYTTGRVGAGVVRGRIFEATSIAEAGAIFEAFEAKAAVGQTPDEGAVPLAGVT
                     D"
     gene            complement(2579..3295)
                     /locus_tag="PLX85_02665"
     CDS             complement(2579..3295)
                     /locus_tag="PLX85_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRC62110.1"
                     /translation="MFARVLVPLDESPHAERALSYAQSILNQHGGQLTLLTVVHTPPA
                     ANPSDPIARLQAERPERARQYLLERAEVLRGAGLNVETKVIEGGDTAEHIVELATTEQ
                     SEVIVMSTHGLGASGRYALGSVALKVLMTAPCPVFMARIQEAWDISPDGQITTEAEAD
                     VGRAETGEERWLAERLAEVDAEIARLDARAPALSDQVANASHASHEQAKWTTLWDLRQ
                     RALLLEERESILRARQALTA"
     gene            complement(3410..>4578)
                     /locus_tag="PLX85_02670"
     CDS             complement(3410..>4578)
                     /locus_tag="PLX85_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62111.1"
                     /translation="AVVLRDCIAKGVGGVGMFTSGFAETDTEEGRAQQQRITEMAREA
                     KLPLIGPNCMGLYHPGLGVRFMENQPAGFEGDVTFLSQSGGHAVDFSMAAHASGVPVR
                     MAISFGNGVILENADYLEYFALDEATRYIAMYVEGLRDGPRFFAALREAARRKPVVLW
                     KGGLTDAGGRATSSHTASLAGAAEVWDAVFRQTGAIPVTSVLEMVDVLKGLRLWPRFS
                     GNGVGLTGGSGGQSVAMADVFSRAGMRVPALSEASRERLASWFSLVGASFNNPVDMGS
                     NRSEMDTILDVLAEDPSIDSLVMQVRPGSGDAADQERLDGQLAALKRLRERSGKPVSA
                     LTSSSMPLEDGPAVADLDGRLREIGVAAYPTYERAAIAMRKVLNYYEFRDSLEG"
BASE COUNT          712 a         1803 c         1379 g          684 t
ORIGIN      
        1 ctcggaggcc gcccccgagg acggagagct ggcaccgctc acctgacctc ggttactcac
       61 tggcggtgga cgcccgccgt cagggggcga tcgtcaccgt gtaggtctgg ctgaccttgc
      121 agatcgtccc gtacacgtag gtcgcgccca gctcgaccgt gaaggtgtac gtaccggcga
      181 ccgttgccgt ccccgaaaga gtgacctggt tgctgctcgg gccaggggtg aacgagagtc
      241 caggcggaag cagcggagcg ttcgtcacgc tcgccggcaa cagattgctc gccggagtga
      301 gcgacagcgt cgaggaatac gcgaacccga cctgcccgtt tggcagccca ttcgggtgca
      361 tgttgacctg gttgcagtgg aacggcggcg cggcctcggt cctcaccgcc cccgtcccga
      421 ggaatgccat cgcgctcgcg agcgccacca tcagcatcac ccgtcgcatc gatgtcccct
      481 ctctccgctc ggcacactct aacccgggtc atgcgaggcg gcattcgcct cgccgcttgc
      541 gctacgccgt cacccgcccc cggtcgcgca ggaatacaga ccacacctcc tgcacgatca
      601 ggattcccag gctcacgagg aggacaccga agatcacttc ggaatgcagg tcctcgccga
      661 gcacgaccca gctgctgagg atgccgaaca gcggctggct gctgtagatc accgagatgc
      721 gcgagggcgg gtacgtcttc agcagccatg cattcgcgat gaacgcgaac ccgccgatga
      781 gcaccccctg gtacagcagc gaccacacca gcggccacgc gaactcgtag tccgtcccct
      841 cgatcggcac gctgacgagg aacaacagga tccccgccgt gaacgcctgc gtcagcacga
      901 tcttcgccag ctcgatccgc tgcgacagga tcgagatcag cacctggctc ccgccgagga
      961 ggaacgacga cacgaagatc agcaggtcgc cgtgcagctg gctaccgccc ttcgccagcc
     1021 ccggcccgaa cgtcacgatg acccccgcgt agctgattac gatccccacc acctgcgccc
     1081 gcccgatgcg gtcgccgctc acgaataggt gcgacaggat cgccgtccag atcgggaacg
     1141 tcgtgaggat cacgacggta tgcgcgacgc tcgtgtggtc ctgccccacg ttcgtcgtcg
     1201 cgattgtcgc cacaaacaac gcgccgagaa gcacgagcgg cagcacctcg ctccgcgccg
     1261 ggagcacccg ccgtctcgaa acgagcgccc acgccagcaa ggcacaccca gcgaggagga
     1321 agcgcgccga cccaatctgc agcggatgga ggtagcgcaa cccgaacttg atcgccgtcg
     1381 ggttcgcccc gaacagcacc gccagcccaa gcgtgaacag cgccgctccg gccgtcaccg
     1441 gccgccgctc gaggggcgcg ggtcgcgcac tcatccgagg agtctagtcg gtcacccccg
     1501 cgagcggcac cgcgccctca tccggcgtct ggccaacggc cgccttcgcc tcgaacgctt
     1561 cgaagatcgc acccgcctcc gcgatcgagg tcgcctcgaa gatccgaccc cgcacgactc
     1621 cggcccccac gcgcccggtc gtgtacagcg acaactgccg tcgcgtgtgc accagcgcct
     1681 cccgaggcgg cagcatcgcc tcgatgagtg acaggtgacg ccgtctggcc gccgcctcgt
     1741 gcgcgcgccg ctccccgacg gggcgagcct cgtgctccgg gtcgaacatc cacggccgcc
     1801 cgagcgcccc tcgccccacc atcactccga ggcagcccgt ggccgccatc atccgttgcg
     1861 cctccgccag cgagcggacg tcgccgttcc ccgtcaccgg gatgcgcacc gcccgcacaa
     1921 cgtccgcgat cacctcccac ggtgcccgcc ccgcgtactg ctgcgacctc gttcggggat
     1981 gcacggtgat ggcgtccacg cccacgtcct cggccattcg cgccacctcg acggcgttca
     2041 ggcacgtgtc gtcccatccg ccgcggatct tgatcgtcag cggtatatcg agggcggtca
     2101 gctcacgccg caacgccccg aggatgcgcg ccgtccgggg cacgtcgcgc atgagcgccg
     2161 cacccttgcc cttcgtcacg gccttcttgg ccgggcaccc catgttgatg tcgatgacgc
     2221 tggcccccgc ctcgacgagc cgcaccgcgg ccggcaccag cgtctcctcc gagttgccca
     2281 gcagctgcat cgccaccggg ccctcgcccg ggtcaatcgc cgtccacacc gccgctttgc
     2341 tcatgcccat cccgagcagc gccgccgcgt cgatctcctc gttggtcgag aaccccgccc
     2401 cgcactcgcg cgcgacgagg cgatacggcg cgttggtgta gctcgccatc ggcgcgagcc
     2461 gcaccggccc cgccgcaatc aggtcgcgca tggacagcat cgaacgagta tcgaggcacg
     2521 ccccgtctcg gacgagcgcg tatggcgcat tggtgccggg cgagggcgac cccgagtgct
     2581 acgccgtgag cgcctgccgc gcccgcagga tcgactcgcg ctcctccagc agcagcgccc
     2641 gctgtcgcag gtcccacagc gtcgtccatt tcgcctgctc gtgcgatgcg tgcgaagcgt
     2701 tcgccacctg gtcgctcagc gcgggtgccc gggcgtccag ccgagcgatc tcggcatcca
     2761 cctccgccag ccgctcggcg agccagcgct cctcgccggt ctcggcccgc ccgacgtcgg
     2821 cctcggcctc ggtggtgatc tgcccgtcag gcgagatgtc ccatgcctcc tggatccgag
     2881 ccatgaacac cgggcatggc gcggtcatca gcaccttgag cgccacgctt ccgagggcat
     2941 accgcccgct cgcgcccagg ccgtgcgtgc tcatcacgat cacctcggac tgctccgtgg
     3001 tcgccagctc cacgatatgt tccgcagtgt cgccaccctc gatgaccttc gtctcgacgt
     3061 tgagcccggc gccgcgcagc acctcggcgc gctcgagcag gtactgtcgg gcgcgctccg
     3121 gccgttccgc ctgcagccgc gcgatcgggt cactcggatt cgcggccggt ggcgtgtgta
     3181 ccaccgtcag cagcgtcagc tgcccaccgt gttggttgag aatgctctgg gcatagctga
     3241 gcgcacgctc cgcgtgcggc gactcatcga gcggtaccag gacgcgagca aacatctgca
     3301 actcctcgat cctcggcggt ggggcgtagt ctcgcacgtg agcgccgcca ctcgcagtgt
     3361 ccaacggccc gtcacgggag ggcccttcgg ccccgccgct gccggggact cacccctcga
     3421 ggctgtccct aaactcgtag tagttcagca ccttccgcat ggcgatggcc gcccgctcgt
     3481 acgtcgggta cgccgctact ccgatctccc gtagccgccc atcgaggtcc gcgaccgccg
     3541 gcccgtcctc cagcggcatc gacgacgaag tgagcgcgct gaccggtttc ccgcttcgct
     3601 cccgcagccg cttgagcgcc gccaactgcc catcgagacg ttcctggtct gccgcgtccc
     3661 ccgacccggg ccgcacctgc atcaccagcg aatcgatgct ggggtcctcc gccagcacat
     3721 cgaggatcgt gtccatctcg ctgcggttcg accccatgtc caccgggttg ttgaagctcg
     3781 cccccacgag gctgaaccag ctcgcgaggc gctcccgtga cgcctcggac agcgccggca
     3841 cccgcatccc cgcccgcgag aacacgtccg ccatcgcaac cgactgcccg cccgatccgc
     3901 ccgtgagccc cacgccgttc ccgctgaagc gcggccacag ccgcaggccc ttaagcacgt
     3961 ccaccatctc gagcaccgag gtcaccggga tcgcgcccgt ctgccggaac acggcgtccc
     4021 agacctcggc cgcccccgcc agcgaggcgg tgtgtgacga ggtcgctcgc ccgcccgcgt
     4081 ccgtcagccc gcccttccac agcaccaccg gcttccgccg cgccgcctcc cgcagcgccg
     4141 cgaagaatcg aggcccgtcg cgcagcccct ccacatacat cgcgatatag cgggtcgcct
     4201 cgtccagcgc gaagtactcg aggtagtccg cgttctcgag gatcaccccg ttcccaaagc
     4261 tgatcgccat ccgcaccggc acccctgagg cgtgcgcggc catcgagaag tccacggcgt
     4321 gcccgccgct ctgcgacagg aacgtgacgt cgccctcgaa tcccgccggc tggttctcca
     4381 tgaaccgcac gccgaggccc gggtggtaca gccccatgca gttcggcccg atcagcggca
     4441 gcttcgcctc gcgcgccatc tccgtgatcc gctgctgctg cgcgcgcccc tcctcggtgt
     4501 ccgtctccgc gaaccccgag gtgaacatcc ccaccccgcc cacgcctttc gcgatgcagt
     4561 cacgcagtac tactgccg
//
LOCUS       DAORPG010000077         4570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1321_length_4570_cov_3.830343, whole genome shotgun sequence.
ACCESSION   DAORPG010000077 DAORPG010000000
VERSION     DAORPG010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1321_length_4570_cov_3.830343"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PLX85_02675"
     CDS             <1..535
                     /locus_tag="PLX85_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRC62112.1"
                     /translation="LLRHDPGWSERAQALSSKVRDVLEFVADQPPGDGPGPIDADVTV
                     QDACHLAHAQRIREAPRAILRSIPGLRVREMHTPDRCCGAAGLYTLVQRDMSQTVLDA
                     KLDDIRATGASVVATSNPGCTLQLEGGLHRAGGDLRVRHVIELLDEAYREGDRQSEAT
                     RQSELSRDSLNSNASNS"
     gene            complement(466..2058)
                     /locus_tag="PLX85_02680"
     CDS             complement(466..2058)
                     /locus_tag="PLX85_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC62113.1"
                     /translation="MSVSRLYGRRELLRLGGASALGMLLAACGNGGLLGDQPKAPSQK
                     DRAGTLRIAPGSGTASPAGPAGLVYSTLVAFDPRRNRLSGDLARSAEVGDNLAITFKL
                     RDNIRFHPDSKNLAAALTAQDVQHDFQERSADREFLFANVIDRVEAPDIETVVLRLRA
                     PFNLLFDYLSDPLTASVRSQTRYQAVNARLGSGPFVPTQRDASGLAMAAHPLFYRAHQ
                     PLLEGLLVADTPVDRDLDAAFVAGDLDVRVHPRDAQPGAAGTRAGATIAKRPSRRMRG
                     LGLSLFPQKSGPAGGNVRFVAAFQDVRVRRAISLTLDRAAIAKVDDGYLSGPIGPAHA
                     GDALSEADLLKHPLYQRDVAEAKKLLDAAGKAGLEFGVEGPNTTTMRNIAQIVDLNLR
                     EAGLAPRVNLVPPDSWAPLFFAGDFEAALFELEDLRTPDIGLRLHLTGGLTGNFSLWG
                     YSDPVYDTALRPVFTKLNPRERGDEARKAQMVLLDQVPAMFPLSAPPEYASLAAEVRG
                     YEFDAFEFNESRLSSLWRVASD"
     gene            2164..2718
                     /locus_tag="PLX85_02685"
     CDS             2164..2718
                     /locus_tag="PLX85_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62114.1"
                     /translation="MDERNEVQRPRSRLKLLASLLLALFAGLVLVGACLSLVVSSTPR
                     HTLEVPTGELSAGAPRFYAQPSFGADANGRTYGVWVLVPDRGATRAFASRAAGSDCSV
                     SWRPEVAVGGVTGVFQDACDNSSYDADGRLLAGPALRGLDRFDVEVTASRVVVDLENA
                     HAGTCRRDPAPGVPCSPEPTATAR"
     gene            complement(2745..3098)
                     /locus_tag="PLX85_02690"
     CDS             complement(2745..3098)
                     /locus_tag="PLX85_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019666336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HRC62115.1"
                     /translation="MADTDTTTDTTETSEGLPTVEELWEVVRQIQDPELRMSIVDLGL
                     VYHVDIDDEGLVTIDLTLTSPGCPVGPMIQGQAYHMLTQIDGVEDVEVNLVWEPPWDP
                     RTMASEEVKMMLGIW"
     gene            complement(3098..3565)
                     /locus_tag="PLX85_02695"
     CDS             complement(3098..3565)
                     /locus_tag="PLX85_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HRC62116.1"
                     /translation="MPASDLEDLYRDILLDHYRNPRNRGRLGAASCSADGANPLCGDQ
                     ITLDVTLDGDRIADIAFDGNGCSISQASASMMTEYVKGRSVEEAHDGIQAFQRMMVEG
                     EPLGEEYGDIEALVGVAKFPVRVKCASLAWKTLEQALSSESARVPVTTDERSQ"
     gene            complement(3552..>4570)
                     /locus_tag="PLX85_02700"
     CDS             complement(3552..>4570)
                     /locus_tag="PLX85_02700"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HRC62117.1"
                     /translation="KVRAFINGYHTAEIVFTRNTTESINLVAHSYGRTFLQPGDEVVI
                     SELEHHSNIVPWQMLRDERGIVLRYIPMFDDGTLDLAEARRLIGPRTKLLAITQMSNA
                     LGTIVPLEELIELAHRNGAVVLVDGAQSVPHLPTDVRALGADFLAFSAHKMLGPTGVG
                     VLWGRRELLEAMPPFLGGGDMILTVSMEQSTWNELPYKFEAGTPNIADVVAFGKALDY
                     LQSFGMDQIRRHERELLEYAVTKLVDLPGITLYGPTDLDQRGGVVSFGLEGVHPHDIG
                     QVLDTHGVAIRTGHHCAQPVMTHLHIPATARASFYMYNTTDEVDALAEAITEAARFFG
                     HARV"
BASE COUNT          704 a         1503 c         1625 g          738 t
ORIGIN      
        1 gctgctcagg cacgaccccg gctggagcga acgagcgcag gccttgtcct cgaaggtccg
       61 agacgtcctc gagttcgtcg cggatcagcc ccccggggac ggccctgggc ccatcgacgc
      121 cgacgtgacc gtgcaggacg cctgccacct cgcgcacgcg cagcggatcc gcgaggcgcc
      181 acgcgcgatc ctgcgctcca tcccggggct gcgcgtgcgc gaaatgcaca cccccgaccg
      241 ctgctgcggc gcggccgggc tgtacacgct ggtgcagcgc gacatgtcgc agacggtcct
      301 cgacgcgaag ctcgacgaca tccgcgcgac cggggcgagc gtcgtcgcga cctcgaaccc
      361 cggttgcacg ctgcagctcg agggcgggct gcaccgcgct ggcggcgacc tgcgtgtgcg
      421 acacgtcatc gagttgctcg atgaagcgta tcgtgagggc gaccgtcagt ccgaggcgac
      481 gcgccagagc gagctgagcc gcgactcgtt gaactcgaac gcatcgaact cgtagccgcg
      541 aacctcggcg gccagtgacg cgtattccgg cggcgccgaa agcgggaaca tcgccggcac
      601 ctgatcgagc agcaccatct gtgctttgcg cgcttcgtcg ccccgctcgc gcgggttgag
      661 cttcgtgaac acggggcgca acgcggtgtc gtacacggga tcggagtacc cccagagcga
      721 gaagttgccg gtcagcccgc ccgtcaggtg cagccgcagg ccgatatcgg gcgtccgcag
      781 gtcctccagc tcgaacagcg ccgcctcgaa gtcgccagcg aagaacagcg gggcccaact
      841 atcgggcggc accaggttga cgcgcggtgc gaggcccgcc tcacgcaggt tcaggtcgac
      901 gatctgggcg atgttccgca tcgtggtggt gttgggcccc tcgacgccaa actcgagccc
      961 cgctttgccg gccgcgtcga gcagcttctt cgcctcggcc acatcgcgct ggtagagcgg
     1021 atgcttgagc agatccgcct cggaaagtgc atcgccggcg tgggcgggcc cgatgggtcc
     1081 gctgagatag ccgtcatcga ccttcgcgat ggccgcgcgg tcgagggtca gcgagatcgc
     1141 gcggcggaca cggacatcct ggaatgccgc gacgaaacgc acgttgcccc ctgcggggcc
     1201 cgacttctgc gggaacagcg acagccccag gccacgcatg cgccttgacg gtcgcttggc
     1261 gatggtagct ccggcccggg tgccggccgc gccgggctga gcatcgcgag gatgcacgcg
     1321 cacgtcgaga tcgccggcca cgaacgccgc gtcgaggtcg cggtcgaccg gggtgtcagc
     1381 tacgagcaga ccctcgagca gcggctgatg cgctcggtaa aacagcggat gggccgccat
     1441 cgcgagaccg gaggcatctc gttgcgtggg cacgaagggg ccgctcccga gcctcgcgtt
     1501 gaccgcctgg tagcgcgtct gcgaccgcac gcttgccgtg agcgggtcgg ataggtagtc
     1561 gaacaggagg ttgaacgggg ctcgtagccg gagcaccacc gtctcgatgt cgggcgcctc
     1621 gacgcggtcg atcacgttcg cgaacaggaa ctcgcggtcg gcggaccgct cctggaagtc
     1681 gtgctgcacg tcctgggccg tcagcgccgc cgcgaggttc ttgctgtccg ggtggaagcg
     1741 gatgttgtcc ctcaacttga aggtgatcgc gaggttgtca cccacctcgg cgctgcgggc
     1801 caggtctccg gaaaggcggt tcctcctcgg gtcgaaggcc acgagcgtcg agtacacgag
     1861 gcccgcaggc cccgctggcg aggccgtccc gctccccggc gcgatccgca gcgtgcccgc
     1921 ccggtccttc tgcgatgggg cttttggctg gtcgcccagc aggccgccgt ttccgcaggc
     1981 cgcgagcaac atgccgagcg ccgaagcccc tccgaggcgc agcaactcgc ggcggccgta
     2041 gagccgggat acggacatcg actcgatcgt cccgttcgcg ctgctggggg gcgggaggtc
     2101 atagtacgga cgggagcaga cgcccgctag ccgctgtgcg tggtcgcggg agacactggc
     2161 ctcgtggatg aacgcaacga ggtgcagcgg ccacgcagca ggttgaagct gcttgccagc
     2221 ctgctgctcg cactcttcgc cgggctggtg ctggtcggcg cctgcctgtc gctcgtggta
     2281 tcgagcacgc cgcgacacac gcttgaagtg ccgacgggcg agctgtcggc tggagcacca
     2341 cgcttctacg cgcagccgag cttcggggcg gacgcgaacg gccgcaccta tggcgtgtgg
     2401 gtgctggtgc ccgatcgggg cgcgacacgc gccttcgctt cgcgcgccgc cggtagtgat
     2461 tgctccgtca gctggcgtcc tgaggtggcg gttggcgggg tgaccggggt gttccaggac
     2521 gcgtgcgaca acagcagcta tgacgccgat ggaaggctgc tggctggacc ggccctgcgc
     2581 gggctcgatc gcttcgacgt cgaggtcacg gcatcgaggg tcgtcgtgga cctcgagaac
     2641 gcgcacgccg gaacctgccg ccgggatccg gcgccgggcg ttccttgctc accggaacca
     2701 acggcaacag cccgctgagg tggaccgggt cgtgggtctg agcgctacca gatccccagc
     2761 atcatcttga cctcctcgga ggccatcgtg cgcgggtccc agggcggctc ccagacgagg
     2821 tttacctcga cgtcctcgac gccgtctatc tgcgtgagca tgtggtaggc ctgaccctgg
     2881 atcatcggac cgacgggaca gcccggcgag gtgagcgtca ggtcgatcgt gaccaggccc
     2941 tcatcgtcga tgtccacgtg gtagacgagg cccaggtcga cgatgctcat gcggagctcg
     3001 gggtcctgga tctggcggac gacctcccag agctcctcga cggtgggcag cccctcggac
     3061 gtctcggtcg tgtccgtggt cgtgtcggtg tcagccatca ctggctcctt tcgtccgtcg
     3121 tcaccgggac gcgggcggat tcgctcgaca gtgcctgttc gagggtcttc caggcgagcg
     3181 aggcgcactt gacgcgcacc gggaacttcg cgacgcccac gagcgcctcg atgtcgccgt
     3241 actcctcgcc gagcggctcg ccctcgacca tcatgcgctg gaacgcctgg atgccgtcgt
     3301 gcgcctcctc gacgctgcgg cccttcacgt actcggtcat catcgaggcc gatgcctgcg
     3361 agatggagca gccgttgcca tcgaaggcga tgtccgcgat gcggtcgccg tcgagagtga
     3421 cgtcgagggt gatctggtcg ccgcacagcg ggttggcgcc gtcagcgctg cacgaggcgg
     3481 cgccaaggcg tccgcgattc ctcggattgc ggtagtggtc gaggaggatg tcccggtaga
     3541 ggtcctcgag gtcagacgcg ggcatggccg aagaacctcg ctgcttccgt gattgcctcg
     3601 gcgagggcat cgacctcgtc ggtggtgttg tacatgtaga aggacgcgcg ggccgtcgcg
     3661 ggaatgtgca agtgcgtcat caccggctgg gcacagtggt ggccggtgcg gatggcgacg
     3721 ccgtgcgtat cgagcacctg accgatgtcg tgcggatgca cgccttcgag gccgaacgag
     3781 acgacgccgc cccgctgatc gaggtccgtc gggccgtaca gcgtgatgcc gggcaggtcg
     3841 accagcttcg tgacggcgta ctcgagcagc tcgcgttcgt gccggcggat ctggtccatg
     3901 ccgaacgact gcaggtagtc gagcgccttg ccgaacgcca cgacgtccgc gatgttgggc
     3961 gtgcccgcct cgaacttgta gggcagctcg ttccaggtcg actgctccat ggagacggtg
     4021 aggatcatgt cgccaccgcc gaggaagggc ggcatcgcct cgaggagttc gcgccggccc
     4081 cagagcacgc cgaccccggt cggaccgagc atcttgtgcg cggagaaggc aaggaagtcg
     4141 gcgcccaggg ctcgcacgtc cgtcggaagg tggggcacgc tctgcgcacc atccacgagc
     4201 acgaccgcgc cattgcggtg cgccagctcg atcagctcct cgagcggcac gatcgtgccg
     4261 agagcgttcg acatctgggt gatggcgagc agcttcgtgc gagggccgat gagccgacgc
     4321 gcctcggcaa ggtcgagtgt gccgtcgtcg aacatcggaa tgtagcggag cacgatgccg
     4381 cgctcatcgc gcagcatctg ccagggcacg atgtttgagt ggtgctcgag ctccgagatc
     4441 acgacctcgt cgcccggttg caggaacgtg cgcccgtacg agtgcgcgac gaggttgatc
     4501 gactcggtcg tgtttcgcgt gaagacgatc tcggcagtgt ggtagccgtt gatgaaggcg
     4561 cgcaccttct
//
LOCUS       DAORPG010000078         4565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1327_length_4565_cov_3.592018, whole genome shotgun sequence.
ACCESSION   DAORPG010000078 DAORPG010000000
VERSION     DAORPG010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1327_length_4565_cov_3.592018"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PLX85_02705"
     CDS             complement(<1..609)
                     /locus_tag="PLX85_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62118.1"
                     /translation="MTHPHAPRPTDLVALISFDGEVFENQAVTREALGKPSSPPHALA
                     ATIQRWLGRGRHVWIDVRGRQIHGIATARPLGSDEAWQIDTLVDASQGEGGVVDGLLA
                     QATEGAVEAGVSRLLLRTRDDAPARDEAQRAGFMHALDEDLYVLDRPARPPHDATSDG
                     VREVEEADLHGLFQLYSRTLPASARQLLGMTFDEWLGARERRW"
     gene            complement(606..1208)
                     /gene="pdxT"
                     /locus_tag="PLX85_02710"
     CDS             complement(606..1208)
                     /gene="pdxT"
                     /locus_tag="PLX85_02710"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007535705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HRC62119.1"
                     /translation="MRPRVGVLAMQGDFREHVERLHALGVEACEVRTEAELNEVDALI
                     IPGGESTTIARLLIAFDLLDPLRARIAAGLPVWGTCAGAIMLAKRVPGLDRPPIGVMD
                     VTVDRNAFGRQVDSFEADLDIAGIEGPPIRAVFIRAPVIREPGPAVEVLAALPDGRIV
                     ACREGRLLATSFHPELTDDSRLHEWFVRLAEEAMASRAPA"
     gene            complement(1240..2169)
                     /gene="pdxS"
                     /locus_tag="PLX85_02715"
     CDS             complement(1240..2169)
                     /gene="pdxS"
                     /locus_tag="PLX85_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HRC62120.1"
                     /translation="MAGSNGNTNNGHGAEQGETATWTVKAGLAQMLKGGVIMDVVTAE
                     HARIAEEAGACAVMALERVPSDIRAAGGVARMSRVELIEEICDSVTIPVMAKARIGHF
                     VEAQILQEIGVDYIDESEVLTPADDQHHIDKHPFNVPFVCGCRDLGEALRRIGEGAAM
                     IRTKGEAGTGDIVEAVRHMRALWSGIRRVQAARDDELAALAKEYQSPLHLIQEVKRLG
                     RLPVVNFSAGGVATPADAALMMQLGADGVFVGSGIFKSDDPARMASAIVRAVTHYNDP
                     TMLASVSRNIGEPMRGRSVASMPVEEQLAGRGN"
     gene            2273..2677
                     /locus_tag="PLX85_02720"
     CDS             2273..2677
                     /locus_tag="PLX85_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRC62121.1"
                     /translation="MNVVIMGCGRVGSTLAARLDRESWHVTIVDTDPYSFRRLPSDFR
                     GEKVVGSGYEDHILVQAGIREADAFLALTQGDNRNLLAAQIAKHIFGVRTVVARVYDP
                     FRGEIFSHLGLRTFSPTNIGAEMAYDALTQQD"
     gene            2691..3377
                     /locus_tag="PLX85_02725"
     CDS             2691..3377
                     /locus_tag="PLX85_02725"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRC62122.1"
                     /translation="MYIIVVGAGKVGHGLGVELIEGGLHEVVLVERDRNRAAELREEL
                     GEAVVHGDGTEVVFLESVGARRADLLIAVTADDGANLVACQVAKHWFHVQRTIARVNN
                     PRNERLFHLLGIDSTISAPGAILAQIEVDLPEHTLIPLMRLHGSGLQLVDLHVLEGSE
                     AAGKTIRDLPLPTETVISLVVTAEGKPQIPTAETVLQPGDEVIAVIRSETEPELRRLV
                     SDAAHARVAD"
     gene            3382..4212
                     /gene="pheA"
                     /locus_tag="PLX85_02730"
     CDS             3382..4212
                     /gene="pheA"
                     /locus_tag="PLX85_02730"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRC62123.1"
                     /translation="MRLAHLGPRGTYTEAAALAFAPDAELMPVATVTAAVAAVEAGQA
                     DAAVCAIENAIDGSINETIDLLLREDGHLHVRGEVVLAIHHALVGPPGLDVSAATVVY
                     SHPSALGQCRRRLAEFAPNARAVAALSTAAAIESSMKETGALAVGNRLSAELYQATIY
                     AEDIADEPGNETRFVVLGHEDAARTGNDKTSIAFTTHHDRPGSLVEVLGIFSRRGINL
                     TRIESRPTRRALGTYVFLVDLLGHRDDPPVAEAIEEARGVTLWMRVLGSYPRWLGTRA
                     "
     gene            4205..>4565
                     /locus_tag="PLX85_02735"
     CDS             4205..>4565
                     /locus_tag="PLX85_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HRC62124.1"
                     /translation="MPEIPDLEAIRGFLEPRLRENPVVGVDLTVPWLVRTGAEGVQGL
                     VGHEFVTLRRYGKFLLFATDDEQLLVVNPMLTGRFQWAEASEKKRAGTCLTLRFEDGH
                     ELRYADARRMGRWYLVPV"
BASE COUNT          693 a         1532 c         1627 g          713 t
ORIGIN      
        1 ccagcggcgc tcgcgcgccc ccagccattc gtcgaaggtc atgccgagca actggcgcgc
       61 gctcgcgggc agcgtgcggc tatacagctg gaagaggccg tgcaggtccg cctcttcgac
      121 ctcgcggaca ccatcggagg tggcgtcgtg cggcggacgg gcggggcggt cgaggacgta
      181 caaatcctcg tcaagggcat gcatgaagcc agcgcgctgt gcctcgtcgc gtgcgggtgc
      241 gtcgtcgcgg gtgcgcagga gcagacggct cacccccgcc tcgacggcgc cttcggtcgc
      301 ctgcgccagc aggccgtcga ccacgccgcc ttcaccctgg ctggcatcga ccagcgtgtc
      361 gatctgccac gcctcgtccg agccgagggg ccgcgcggtg gcgatgccat ggatctgacg
      421 gccgcggaca tcgatccaca cgtggcgacc gcgcccgagc cagcgctgaa tcgtggcggc
      481 gagggcatgc ggtggtgacg agggcttgcc cagtgcctcg cgggtcacgg cctggttctc
      541 gaacacctca ccgtcgaacg agatcagcgc gacgaggtcg gtcggtcgag gggcatgagg
      601 atgggtcacg cgggcgccct gctggccatc gcctcctcgg caaggcggac gaaccattcg
      661 tggaggcggg agtcgtcggt gagctccggg tggaacgagg tggcgaggag gcggccctcg
      721 cggcaggcga cgatgcgacc gtcgggaagg gcagccagca cctcgacggc ggggccgggc
      781 tcgcggatga ccggggcgcg gataaagacg gcgcggatcg gtgggccctc gatgcctgcg
      841 atgtcgaggt cggcctcgaa cgagtcgacc tggcggccga aggcgttgcg gtcgacggtc
      901 acgtccatca ctccgatggg cggacggtcg agtccgggga cccgctttgc gagcatgatc
      961 gcgccggcgc acgtgcccca tacggggagc cccgccgcga tgcgggcgcg cagcgggtcg
     1021 agaaggtcga aggcgatgag gaggcgggcg atggtcgtgc tttcgccgcc ggggatgatc
     1081 agcgcgtcga cctcgttgag ctcggcctcg gtccgtacct cgcaagcctc gacgccgagg
     1141 gcatgcagcc gctccacgtg ttcgcggaaa tcgccctgca tcgcgaggac gccaacgcgg
     1201 ggacgcacga ggttcgtcgg gcctccttcg tgtggagtgt cagttgcccc ggccggcgag
     1261 ttgttcctcg accggcatag aggcgacgga gcggccgcgc atcggctcgc cgatgttgcg
     1321 cgacacgctc gcgagcatcg tggggtcgtt gtagtgcgtg acggcgcgga cgatggcgga
     1381 ggccatgcgc gcggggtcgt ccgacttgaa gatgccggag ccgacgaaca cgccgtcggc
     1441 gccgagctgc atcatcaacg ccgcgtcggc gggtgtggcg acgccaccgg cggagaagtt
     1501 cacgaccggc aaccggccga ggcgcttcac ttcctggatg aggtgcagcg gggactggta
     1561 ctccttggcg agcgcggcga gctcatcgtc gcgggcggcc tgcacgcggc gaatgccgct
     1621 ccagagtgcg cgcatgtggc gtacggcctc gacgatgtcg ccggtgccgg cctcaccctt
     1681 ggtgcggatc atcgcggcgc cctcgccgat gcggcgcagc gcctcgccga ggtcccggca
     1741 gccgcagacg aacggcacgt tgaatgggtg cttgtcgatg tggtgctggt catcggcggg
     1801 cgtcaggacc tcggactcgt cgatgtagtc gacgccgatc tcctggagga tctgtgcctc
     1861 cacgaagtgg ccgatacgcg ccttcgccat gaccgggatc gtgacggagt cgcagatttc
     1921 ctcgatgagc tcaacccggc tcatgcgggc gacgccgccc gcggcgcgaa tgtcgctcgg
     1981 gacgcgctcg agggccatga cggcgcaggc gccggcctcc tcagcgatgc gggcgtgctc
     2041 ggcggtgacc acgtccatga tcacgccgcc cttgagcatc tgcgcgaggc cggcctttac
     2101 ggtccaggtc gcggtttcgc cctgctccgc accgtgcccg ttgttcgtgt tgccgttgct
     2161 gcccgccata gcgctccccg cgtccacatt cggtccgtgg cgttattgta ccccgccgct
     2221 cgcggggccg ctgccagtac cgcgcgcgcg aacggtggca taccatcacg gcatgaacgt
     2281 ggtgatcatg ggttgcggac gcgtcggatc gacactggcc gcgcggctcg accgcgagtc
     2341 gtggcacgtc accatcgtcg acacggaccc gtactcgttc cgccgtctgc cctcggactt
     2401 tcgaggtgag aaggtcgtcg ggtcgggcta cgaggatcac atcctggtgc aggccggcat
     2461 ccgcgaagcc gacgccttcc tcgcgctgac gcagggcgac aaccgcaacc tgttggctgc
     2521 ccagatcgcg aagcacatct tcggcgtgcg caccgtggtc gcgcgcgtgt atgacccttt
     2581 ccggggtgaa atcttcagcc acctcggact gcggacgttc agccccacca acatcggcgc
     2641 cgaaatggcg tacgacgcac tgacccagca ggactagccg gagccgcaac gtgtacatca
     2701 tcgtcgttgg cgccgggaaa gtcgggcatg ggctcggggt cgaactgatc gaaggcggac
     2761 tgcacgaggt cgtgctcgtc gagcgcgacc gcaatcgggc tgcggagctg cgcgaagagc
     2821 tgggcgaggc cgtcgtgcac ggcgatggga ccgaggtggt attcctcgag agcgtgggcg
     2881 cgaggcgggc agatctgctg atcgcggtga ctgccgacga cggcgcgaac ctcgtggcgt
     2941 gccaggtggc aaagcactgg ttccacgtcc agcgcacgat cgcgcgcgtg aacaacccgc
     3001 gcaacgagcg gctgtttcac ctcctcggta tcgactccac gatctccgca ccgggcgcca
     3061 tcctcgcgca gatcgaagtg gacctgccgg agcacaccct catcccgctg atgcgtctgc
     3121 acggctcggg cctgcagctc gtcgacctcc acgtcctcga gggctccgag gcggcgggca
     3181 aaacgattcg cgatctgccg ctgccgaccg agacggtcat ctcgctcgtc gtgaccgccg
     3241 aggggaagcc gcagatcccg accgccgaga ccgtgctgca acccggcgac gaggtgatcg
     3301 ccgtcatccg atcggaaacg gagccggagc tgcgacgact cgtcagcgat gccgcgcacg
     3361 cgcgagtcgc ggactagccc gatgcgcctc gcgcacctcg ggccccgcgg cacgtacacc
     3421 gaggccgctg cccttgcctt tgcgccggat gccgagctga tgccagtggc gaccgtgacc
     3481 gcggcggtcg cggcagtcga agcgggacag gcggacgccg ccgtctgcgc catcgagaac
     3541 gcgatcgacg gcagcatcaa cgagacgatc gacctgctgc tgcgcgagga cggccacctg
     3601 cacgtccgcg gcgaggtagt gctcgcgatc catcacgccc tggtcggccc gcccgggctc
     3661 gacgtgtcgg cggcaaccgt cgtgtactca catccctcgg cgctcgggca atgtcgtcga
     3721 cggctggccg agttcgcgcc gaacgctcgc gcggtcgccg cgctctcgac ggccgctgcc
     3781 atcgagtcct cgatgaagga gaccggcgcg cttgcggtgg gaaaccggct gtcggcggag
     3841 ctctatcagg ccaccatcta cgccgaggac atcgcggatg aaccgggcaa tgagacccgg
     3901 ttcgtcgtgc tcgggcacga ggacgccgcg cgcacgggga acgacaagac ctcgatcgcg
     3961 ttcaccacgc accacgaccg ccccggctcg ctcgttgagg tgcttggcat cttctcgagg
     4021 cggggcatta acctcacccg aatcgagtcc cgtccgacgc gccgcgccct ggggacgtac
     4081 gtgttccttg tcgacctact gggccatcgg gacgacccgc cggtcgccga ggcgattgag
     4141 gaggcacgcg gcgtgacgct ctggatgcgc gtgctcgggt cgtatccgcg ctggctcggg
     4201 acgcgtgcct gagatccctg acctcgaagc gatccgcgga ttcctcgagc cgcggctgcg
     4261 cgagaacccg gttgttggcg tcgacctaac ggtgccctgg ctcgtgcgca ctggtgcaga
     4321 gggcgtccag gggctcgtgg gtcatgagtt cgtgacactg cgccgctacg ggaagttcct
     4381 gctgttcgcg accgacgacg agcagctcct cgtggtgaac ccgatgctga ccggtcgctt
     4441 ccagtgggcc gaggcgagcg agaagaaacg tgccggcact tgcctgacgc tacgctttga
     4501 ggacggtcat gaactccgct acgcggacgc ccgccggatg ggccgctggt acctcgtgcc
     4561 cgtcg
//
LOCUS       DAORPG010000079         4534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1355_length_4534_cov_3.400089, whole genome shotgun sequence.
ACCESSION   DAORPG010000079 DAORPG010000000
VERSION     DAORPG010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1355_length_4534_cov_3.400089"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PLX85_02740"
     CDS             complement(<1..648)
                     /locus_tag="PLX85_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62125.1"
                     /translation="MSSTDERVEQRTRISEAARALLEAAPWDEIASRTLLVLVRPPDV
                     EWAATSAPPLLWLILDGAEARALRPELRNPLVRDGSTLVHVRATDAAPAMQLAAMTSE
                     AVGRHLEGVTRRSLEMKWLARYSEALHDPLHRFDSLRGMAARLPADALERVARPLYVQ
                     AAQSLEALAGAPMAAEGADMTKTTHGKVRGRTIELTEDLGLQDGQDVRVQVTPVPT"
     gene            complement(645..1151)
                     /locus_tag="PLX85_02745"
     CDS             complement(645..1151)
                     /locus_tag="PLX85_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRC62126.1"
                     /translation="MSATVLVIYDSREGLVAELARAVCAGVDSVDGVELRARTVDEAS
                     PEELIEADGLILGSPNWTGITGKLKEWMDRSGDLWESGELAGKPGGAFTAGWSPHGGL
                     EATLLQLLHLILGHGMVFVGLPWSERMRRSGSYYGATAHASVTNDDREQAHALGRRVA
                     EFAVRLQS"
     gene            complement(1155..1415)
                     /locus_tag="PLX85_02750"
     CDS             complement(1155..1415)
                     /locus_tag="PLX85_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62127.1"
                     /translation="MNERQQLRFALAALAAGVAWGYFRHRGGKHPITHAVLQGIEWFV
                     AYGGAVALISGLRERLQEVEPEIEERITHFRQVVTEHTGTDG"
     gene            1613..2494
                     /locus_tag="PLX85_02755"
     CDS             1613..2494
                     /locus_tag="PLX85_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HRC62128.1"
                     /translation="MQDVTYRRPATLQEAVQLLKDNGPTARVLAGGTDIIVMARERRR
                     DTSMFVDIKAIPELTGIRFDASEGLTLGAATPLYQIYRNETIQRLYPCLVDSASLIGG
                     TAVQGRASFGGNLCNAGPAADSIPSLIVLEGKARIVGPNGERTVAVEDFCTAPGRNVL
                     EAGEILVSLSFPAPQPNSGARFLRFIPRNEMDIAVVNAATSLRFEGNNVVAARIAIGA
                     VAPTPLFVPAAGAALVGKPLTDESIAAAASASRDAATPIDDMRGSVRQRKHLSYVLTE
                     RTIRDAAHRARGEALEH"
     gene            2498..2971
                     /locus_tag="PLX85_02760"
     CDS             2498..2971
                     /locus_tag="PLX85_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRC62129.1"
                     /translation="MSNRIIINTVINGEPTEFLADERDSLLEALRERIGLTGVKEGCN
                     NGNCGACAVIMDGRLVNSCLVLAAETEGANITTVEGLAKGGELTPLQQTFLEDAALQC
                     GICTPGFLVAATALLEQNPHPSEHEIRYWLAGNLCRCTGYDKIIRAVQHAAERSA"
     gene            3089..>4534
                     /locus_tag="PLX85_02765"
     CDS             3089..>4534
                     /locus_tag="PLX85_02765"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRC62130.1"
                     /translation="MVATEPYKIVGTRPVRPDGVDKVTGRAAYGSDIKLPGMLYARVK
                     RSPHAHAIIKKIDASKALALPGVKAVVTRADFREAGPGLIDIGEGPPVNLKFMLDNVC
                     AGDKVVYKGHAVASVAATDLHTAEDALNLIEVEYEVLPPVMNVRDAMLESAPLIHGNE
                     LTGGLTAADMGKRPSNIANRVEFEMGDVDAGFAQSDIVIEREFETAMYHQGYIEPHNA
                     TALWNKDGDITIWTSTQGTFSVRTASAMILGVPVSSIKVVPMEIGGGFGGKIPVYLEP
                     HAALLSKKLGGVPVKMWMTREEVLEATGPTSGTYSRVKIGAKNDGTLMAANVHLAFEA
                     GGFPGSPVGAGAMCALGPYSIPNAFVEGFDVVVNRPKVAAYRGPGAPASEYAVESVID
                     EIAERIGMDPMDMRLKNAAVEGTRRPDGATMGVIGNVQVMEAIKDSPHYKSELEGENR
                     GRGVAMGFWFNAGMESAAYATVVSDGTVALVT"
BASE COUNT          741 a         1761 c         1350 g          682 t
ORIGIN      
        1 ggtcggcacg ggggtcacct ggacgcgcac gtcctgcccg tcctgtaagc ccaggtcctc
       61 ggtcaactcg atcgtccggc cgcggacttt gccgtgcgtc gtcttggtca tgtcggcgcc
      121 ctccgccgcc atcggcgccc ccgcgagggc ctcgagcgac tgcgccgcct gcacgtacag
      181 cggccgcgcc actcgctcca gcgcatccgc cggcagccgt gctgccatcc cccgcaagga
      241 gtcgaaacgg tgcagcgggt cgtgcaacgc ctcgctgtac cgcgcgagcc acttcatctc
      301 gagcgatcgc cgcgtcaccc cttcgaggtg tcgcccgacc gcctccgagg tcatcgccgc
      361 cagctgcatc gcgggggcgg catcggtcgc gcgcacgtgc accagcgtcg acccatcgcg
      421 caccagcgga ttccgcaact cgggccgcag cgcccgcgcc tcggccccat cgaggatcaa
      481 ccacagcagc ggcggcgcgc tcgtcgcggc ccattccacg tcgggcggtc gcacgagcac
      541 cagcaacgtc cgcgaggcga tctcgtccca cggcgcggcc tcgaggagcg cgcgtgccgc
      601 ctcactgatc cgagtccgct gctccacccg ctcgtcggtc gagctcacga ctgcagcctg
      661 acggcgaact ccgccacccg ccgtccgagg gcgtgggcct gctcgcggtc gtcattcgtc
      721 acactcgcgt gcgccgtcgc cccgtagtac gagccagacc gccgcatccg ttccgaccag
      781 ggcagcccca cgaacaccat cccgtgcccg aggatcaggt gcagcagctg cagcagggtg
      841 gcctccagcc ccccgtgcgg cgaccagcct gcggtgaacg cgccgcccgg cttccccgcc
      901 agctcacccg attcccacag gtctccgctg cggtccatcc actccttcag cttcccggta
      961 atgcccgtcc agttcggcga cccgaggatc aacccgtccg cctcgatcag ctcctctgga
     1021 ctcgcctcgt cgaccgtccg cgctcgcaat tcgacaccgt cgaccgagtc cacgcccgcg
     1081 cacacggcgc gcgccagctc ggctacgagc ccctctcggc tgtcgtagat cacaaggacc
     1141 gtggctgaca tggtctagcc gtcggtgccg gtgtgctcgg tcaccacctg ccggaagtgc
     1201 gtgatccgct cctcgatctc cggctccacc tcctgcagcc gctcccgcaa ccccgagatc
     1261 agcgccaccg ctcccccgta agccacgaac cattcgatgc cctgcagcac ggcgtgcgtg
     1321 atcgggtgct tcccgccgcg atggcggaag tatccccagg ccaccccggc ggcgagcgcc
     1381 gcgagggcaa atcgcagctg ttgtcgttcg ttcacccgga ctcctcggct ccatcgtacg
     1441 ggtgccgcgc accccccgta cagcgcgacg cgacggcgcg tcggacagga cgaacgtcag
     1501 tagcggacga tgtcccaacg tggcagacta cgcgcgtcgt actacacgtg cctgataggt
     1561 gtatgctgcg cggcggcccg cagggccgtg cagcacgaga aggagaggcg atttgcagga
     1621 cgttacatac cgtcggcccg ccaccttgca agaggcggtg caactgctca aggataacgg
     1681 ccctacggct cgtgtcctcg cgggcggcac ggacatcatc gtcatggccc gggagcggcg
     1741 ccgtgacacc agcatgttcg ttgacatcaa ggccatcccg gagctgacgg gcattcgctt
     1801 cgacgcgagc gaaggcctca ccctcggcgc cgccacgcct ctctaccaga tctaccgcaa
     1861 cgagacgatt cagcggctct acccctgcct cgtcgactcc gcctcgctca tcggcggcac
     1921 cgccgtccag gggcgcgcca gcttcggtgg caacctctgc aacgccggtc cggccgccga
     1981 cagcatcccc tcgctcatcg tcctcgaggg caaggcgcgc atcgtcgggc cgaacggtga
     2041 acgcaccgtc gccgtcgagg acttctgcac cgctccaggc cgcaacgtcc tcgaggccgg
     2101 tgagatcctc gtctcgctga gcttcccggc tccgcagccc aacagcggcg ctcgcttcct
     2161 ccgcttcatc ccccgcaacg agatggacat cgcggtcgtc aacgccgcca cgtccctccg
     2221 cttcgagggc aacaacgtcg tcgccgctcg catcgccatc ggcgccgtcg cgcccacgcc
     2281 gctcttcgtc cccgccgctg gcgccgctct cgtgggcaag ccgcttacgg acgagtccat
     2341 cgccgccgcc gccagcgcat cgcgcgacgc cgcgacgccc atcgacgaca tgcgcggttc
     2401 cgtccggcag cgcaagcacc tctcgtacgt cctcaccgag cggaccatcc gcgacgcggc
     2461 tcaccgcgct cgtggcgaag cgttggagca ctaagccttg agcaaccgca tcatcatcaa
     2521 caccgtcatc aacggggagc cgaccgagtt cctcgctgac gagcgcgaca gcctcctcga
     2581 ggcgctgcgt gagcgcatcg gactcaccgg cgtcaaagag ggatgcaaca atggcaactg
     2641 cggcgcctgc gccgtgatca tggacgggcg cctggtcaac tcctgcctcg tcctcgccgc
     2701 cgagaccgag ggcgccaaca tcaccaccgt cgagggcctc gcaaagggtg gtgagctcac
     2761 ccccctgcag cagaccttcc tcgaggatgc cgccctccag tgcggtatct gcactcccgg
     2821 cttcctcgtc gccgccaccg cgctcctcga gcagaacccc caccccagcg agcacgagat
     2881 ccgctactgg ctcgccggca acctctgccg ctgcaccggc tacgacaaga tcatccgcgc
     2941 cgtccagcac gccgcggagc gctccgccta gccgcgcata tacatacgtg gttcgctgga
     3001 tacggtacgt cgaataaacg caccgtatcc agtagaatgt cggccaacca cccggcagtc
     3061 gctcacttcg acgttaggag actcctgcat ggtcgcgact gagccataca agattgtcgg
     3121 cacccgcccc gtccgccccg atggggtgga caaggtcacc ggacgtgccg cctacggctc
     3181 tgacatcaag ctccctggca tgctctacgc gcgcgtcaag cgcagcccgc atgcccacgc
     3241 catcatcaag aagatcgacg cctcgaaggc cctcgccctc ccgggcgtca aggccgtcgt
     3301 cacccgcgcc gacttccgcg aggctggccc cggactcatc gacatcggtg agggcccccc
     3361 ggtcaacctc aagttcatgc tcgacaacgt ctgcgctggc gacaaggtcg tctacaaggg
     3421 ccacgcggta gcgtccgtcg cggccaccga cctccacacc gccgaggacg ccctcaacct
     3481 catcgaggtt gagtacgagg tgctcccccc ggtcatgaac gtccgcgacg cgatgctcga
     3541 gtccgcgccg ctcatccacg gcaacgagct caccggtggc ctcaccgccg ctgacatggg
     3601 caagcgcccc tcgaacatcg ccaaccgcgt cgagttcgag atgggcgacg tcgacgccgg
     3661 cttcgctcag tccgacatcg tcatcgagcg cgagttcgag accgcgatgt accaccaggg
     3721 ctacatcgag ccgcacaacg ccaccgccct ctggaacaag gatggtgaca tcaccatctg
     3781 gaccagcacc cagggcacct tctcggtgcg cacggccagc gccatgatcc tcggggtccc
     3841 cgtgtcgtcc atcaaagtcg tcccgatgga gatcggtggc ggcttcggcg gcaagatccc
     3901 ggtctacctc gagccgcacg cggcgctcct ttcgaagaag ctcggcggcg tcccggtcaa
     3961 gatgtggatg acccgcgagg aagtcctcga ggccaccggc ccgacctccg gcacctacag
     4021 ccgcgtcaag atcggcgcca agaacgacgg caccctcatg gccgccaacg tccacctcgc
     4081 cttcgaggcg ggcggcttcc cgggctcccc ggtcggcgcc ggcgccatgt gcgccctcgg
     4141 cccctactcg atcccgaacg ccttcgtcga ggggttcgac gtggtcgtga accggccgaa
     4201 ggtcgccgcc taccgcggtc ccggcgcgcc ggcgtccgag tacgccgttg aatcggtcat
     4261 cgacgaaatc gccgagcgca tcggcatgga cccgatggac atgcggctca agaacgctgc
     4321 cgtcgagggc acccgtcgcc cggacggcgc caccatgggc gtcatcggca acgtccaggt
     4381 catggaagcc atcaaggaca gcccgcacta caagtcggag ctcgagggtg agaaccgcgg
     4441 ccgtggtgtc gccatgggct tctggttcaa cgccggcatg gagtccgccg cctacgcgac
     4501 cgtcgtctcc gacggcaccg tcgccctggt cacc
//
LOCUS       DAORPG010000080         4518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1360_length_4518_cov_3.668833, whole genome shotgun sequence.
ACCESSION   DAORPG010000080 DAORPG010000000
VERSION     DAORPG010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1360_length_4518_cov_3.668833"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="PLX85_02770"
     CDS             <1..565
                     /locus_tag="PLX85_02770"
                     /inference="COORDINATES: protein motif:HMM:NF022747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HRC62131.1"
                     /translation="FGGFLTLGAVAIALVVVAAIFVLNRGGDDSSQTSTDVSADGLMG
                     SAVAATDPKTDRQHISDPLQLQIPKGEPPTRGPHFAVPQNTGVYKQAVPDGNAIHALE
                     HGIVWISYNPSKVDADTIKKLEDLGKQYAVDLIVSPRPDNDRAIDIVSWGQILVLDKF
                     DRGTLEKFITTNRNRAPEPFVRTTTSM"
     gene            614..916
                     /locus_tag="PLX85_02775"
     CDS             614..916
                     /locus_tag="PLX85_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62132.1"
                     /translation="MPSELAVAFDEMTVLTEGGVDVFLLNMDEMEDVPPYYIDVAGRR
                     FSYSSTTFPTKGHSAQLPRFIQEQEAAGNLVVLGERKNRYIAYVHDPNAVEDEEGE"
     gene            complement(993..2486)
                     /locus_tag="PLX85_02780"
     CDS             complement(993..2486)
                     /locus_tag="PLX85_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62133.1"
                     /translation="MRRGWANIAIAAVLTVTAVAVLMAGRTPRGAEAATTLYVTSSAD
                     SGAGTLRAAITSANSDSSVNLIVLKNWLPVTLFTSVEYTGTQALTIQGDQPLVYRTIQ
                     GSLTVPGDPNSEPLDCGMFKSTGGADITFSQVTIQNSWCDALNIVPVVGDSEITITFN
                     KVNLRNNFGSGMFLHEIEAAEATVRLNVTSSQVVGNGRCRGSMGCAFGGIELQGLGEG
                     EVVVQLAGTWFNGNGGDGMSIGWDAPEVTTLPSPALDVDISVTASNTTFNDNGGTVTY
                     AAALGEGSRGRASTASHQGGEGLGVFGSGLGTGDVTLALTNVQANRNGGSGIRVFRGS
                     DIDVGDVRFTANTLTASNNGGAGIQLEDEGDGDVIVSLTLVSAWFNQGPGVDERESGE
                     GGVDLEARNSSVGFNHLDGFRLEESGDGDFSARFNGTVVSGNAGDGLDMEEHDSGAAS
                     AAVQSSSIFGNGESAIEFEAHDEFGTISIGVSFIVGGVDLDHVNQLF"
     gene            complement(2674..4131)
                     /locus_tag="PLX85_02785"
     CDS             complement(2674..4131)
                     /locus_tag="PLX85_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62134.1"
                     /translation="MNRGAWTAAVIAVLGVVMGGVLLSSQRASTAEAATAFVTSSADS
                     GPGTFRAAIEAANASSSVNLIVLTNTLPVQLTSQVTYTGNQDLVIQGNSVLSNRVIQG
                     DGYGGNAVAEGCDALFRSSGGARNLTFQSVTFQNTYCSAVVIYASDSSQTTRTVTLTK
                     TIIQHADGYGLEMYEGGSPAATSWVLTMTNSHVEDTALCFGDGVAVYEHGWGGIRATI
                     TGSSSSNNGGTGLDLEEEDDGDISLTATMSSFNGNGEVDPESVSTAGADDDSTLVGAL
                     ADSGPTAQCCDYDGINVDENDLGRVTIVLQTVVQASGNAGHGINVHENYDGDISFTAS
                     GVIANDNCNDGIHVREYGNGSLSAQLVNVTTAENWDDGTDIDENGNGNLRVQATASSA
                     NSNGDDGYDLDEGCDGSATFVFTVVSANNNDDDGIDLSEEQNGAFNATLAGSFVQGNG
                     DYGIEAYAENETGLLNWGGSIVLGGIYASGVTVIP"
BASE COUNT          739 a         1713 c         1286 g          780 t
ORIGIN      
        1 cttcggcggc ttcctcacgc tcggcgcggt cgccatcgcc ctcgtcgtcg tggcggcgat
       61 cttcgtcctc aaccgcggcg gcgacgactc ctcccagacc tccacggacg tctccgcgga
      121 cggcctcatg ggctccgccg tcgcggccac cgaccccaag accgaccgcc agcacatcag
      181 cgacccgctg cagctccaga tcccgaaggg tgagccaccc acgcgaggcc cgcacttcgc
      241 cgtgccccag aacaccggcg tctacaagca ggccgtcccg gacggcaacg ccatccacgc
      301 cctcgagcac ggcatcgtct ggatcagcta caacccctcc aaggtcgacg ccgacaccat
      361 caagaagctc gaagacctcg gcaagcaata cgccgtcgac ctgatcgtct ccccccggcc
      421 cgacaacgac cgtgccatcg acatcgtctc ctggggccag atcctcgtcc tcgacaaatt
      481 cgacaggggc accctcgaga agttcatcac caccaaccgc aaccgcgcac cagagccgtt
      541 cgtgcgtacc accacgagca tgtagtctcg cgctgacgag gcagcatcca cccgaggagg
      601 aggacgaccc atcatgccca gcgaactcgc cgtcgccttc gacgaaatga ccgtcctcac
      661 cgagggcggc gtcgatgtct tcctcctcaa catggacgag atggaagacg tcccccccta
      721 ctacatcgac gtcgccggac gccgcttctc ctactcctcc accaccttcc ctaccaaggg
      781 acacagcgcc cagctccccc gcttcatcca ggagcaggaa gccgcgggca acctcgtcgt
      841 cctcggcgag cgcaagaacc gctacatcgc ctacgtccac gaccccaacg ccgtcgagga
      901 cgaagagggc gagtagccgc cacttccggt ccaagaacgc agaaggggcg ccatgcggcg
      961 cccctttatg ctgcctgatg tcccggcccc cgttagaaca actggttcac gtggtccagg
     1021 tcgacgcctc cgacgatgaa cgagaccccg atcgagatcg tcccgaactc gtcatgcgcc
     1081 tcgaactcga tcgccgactc gccgtttccg aagatcgagg acgactgcac cgctgcgctc
     1141 gccgcgccgc tgtcgtgctc ctccatgtcg agcccgtcgc ccgcgtttcc gctcaccacg
     1201 gtcccgttga accgggctga gaagtcccca tcgccggact cctcaaggcg gaacccgtcg
     1261 agatggttga agcccaccga ggaattcctg gcctcgaggt caacgccgcc ttcgccgctc
     1321 tcacgttcgt ccaccccggg cccctggttg aaccacgcgg acaccaacgt gagcgacaca
     1381 atgacgtctc cgtccccctc gtcctcgagc tggattccgg ccccgccatt gttcgacgcg
     1441 gtgagggtgt tcgcggtgaa ccgcacgtca ccgacgtcga tgtccgaacc tcggaacacc
     1501 cggatcccac tgccgccgtt ccgattcgcc tgcacgtttg tcagcgccag cgtcacgtcg
     1561 cccgtcccga gcccgctccc gaacacgccg aggccctcgc ccccctgatg cgacgccgtc
     1621 gaggcacgcc cgcggctccc ctcgcccaga gcggcggcgt acgtaaccgt ccccccgttg
     1681 tcgttgaacg tcgtgttgct cgcggtcacg ctgatgtcta cgtccagcgc cggactcggc
     1741 agcgtcgtca cctccggcgc gtcccacccg atggacatgc cgtccccgcc gttcccgttg
     1801 aaccacgtcc cggcgagctg caccacgacc tcgccctcgc cgagcccctg gagctcgatg
     1861 ccgccgaagg cgcaccccat cgaccctcgg caccgcccgt tccccaccac ctgcgacgag
     1921 gtgacattga gccgtacggt cgcctcggcc gcctcgatct cgtgcaggaa cattccggaa
     1981 ccgaagttgt tccgcaggtt caccttgttg aaggtgatcg tgatctcgct atcgcccacc
     2041 accggcacga tgttgagggc gtcgcaccac gagttctgga tcgtcacctg gctgaaggtg
     2101 atgtccgcac cgcccgtgct cttgaacatc ccgcagtcca gcggttccga gttcgggtcg
     2161 cccgggacag tcagcgaccc ctggatcgtg cggtatacga gcggctgatc gccctggatc
     2221 gtcagcgcct gcgtccccgt gtactccacc gaggtgaaaa gtgtgaccgg cagccagttc
     2281 ttcagcacga tcaggttcac gctcgaatca ctgttggccg acgtgatcgc ggcccgcagc
     2341 gtccccgccc ccgagtccgc cgaggacgtc acgtacagcg tcgtcgccgc ctccgccccg
     2401 cgaggcgtgc ggccggccat cagcaccgcg actgcggtga cggtgagcac ggccgcgatc
     2461 gcgatgtttg cccatcctcg tctcatcgat gctctccctc ggcacgtgac ctcgccgtcg
     2521 cggtttcgtg ctcgttcata cgcgccccgg cgggcgctcg tcaaacaata ggcaggcgct
     2581 aggcgcagct gcgggaccgc ctgcgagccc gcgtcacgcg aaaaaaggag gggcgcacga
     2641 gcgcccctcc tgattcagcg gcctacccgc cagttacgga atcacggtca cgcccgacgc
     2701 atagatgccg ccgaggacga tcgacccgcc ccagttcagc agccccgtct cgttctcggc
     2761 gtatgcctcg atcccgtagt cgccattccc ctgcacgaag gaaccggcaa gcgtcgcgtt
     2821 gaacgccccg ttctgctcct ccgacaggtc aatgccgtcg tcgtcgttgt tgttggccga
     2881 cacgaccgtg aagacgaagg ttgcgctccc atcgcagccc tcatcgaggt cgtacccgtc
     2941 gtcgccattc gaattggcgg acgatgcggt cgcctgcact cgcaggttcc cgttgccgtt
     3001 ctcgtcgatg tccgtcccgt cgtcccagtt ctccgccgtc gtgacattca ccagctgcgc
     3061 cgacagcgac ccgtttccgt actcacgcac gtggatcccg tcgttgcagt tgtcgttcgc
     3121 gatcacgccc gacgcggtga acgagatgtc gccgtcgtag ttctcgtgca cgttgatgcc
     3181 gtggcccgcg ttgccgctgg cttgcacgac cgtctggagc acgatcgtca ccctgccgag
     3241 gtcgttctcg tcgacgttga tgccgtcgta gtcgcagcac tgcgcggtcg gcccggaatc
     3301 ggccagcgcg cccacgagcg tggaatcgtc atcggcgccg gcggtggaca cggattccgg
     3361 gtccacttcg ccgttcccgt tgaagctgct catggtcgcc gtgaggctga tgtcgccatc
     3421 gtcctcttct tcgaggtcga ggcctgtccc gccgttgttg ctcgacgaag acccggtaat
     3481 ggtggcccgt attccgcccc agccatgctc gtacacggcg acgccgtcac cgaagcacag
     3541 cgcggtgtcc tccacgtgcg agttcgtcat ggtcagcacc cacgaggtcg cggcggggct
     3601 gccaccctcg tacatctcga ggccgtaccc atcggcgtgc tgaatgatcg tcttcgtcag
     3661 cgtcacggtc cgcgtcgtct gagacgagtc actcgcgtag atcacgaccg cactgcagta
     3721 cgtgttctgg aacgtgaccg actggaacgt gaggttcctg gcgcctccgc tgctccggaa
     3781 gagcgcatcg cacccctccg cgaccgcgtt cccgccatag ccgtcgcctt ggatcacgcg
     3841 gttcgacagc acgctgttgc cctggatcac caggtcctgg ttgccggtgt acgtcacctg
     3901 gctggtcagt tggaccggca gggtgttcgt cagcacaatc aagttgacgc tcgagctggc
     3961 attggcggcc tcgatggccg cccggaacgt tcccggcccg gagtccgccg aactggtgac
     4021 aaacgccgtc gccgcctccg cggtggaggc gcgctgcgaa ctgagcagga cgcctcccat
     4081 caccactccc aggaccgcga tcaccgccgc ggtccacgcc cctctgttca tgcgatccct
     4141 cctgacgccg tcagacggcg aatcgcctcc cgcctcagga ggcgcatacg gccgttgtat
     4201 ccacttactg tcgccgagtc aatggcggag catccgcgag attagaggtg caccggctgc
     4261 ctttcgcccc ctcggtcccc atgacgccac gatccgagga gccaacctcc gaaaacgctc
     4321 aggacccctc ctcccgcgac aaggccctcg tcccatcccc taacgacgcc gggtaccacc
     4381 gacccgtccc gtcccggcac gcggaagggg cgccccgcgg cgcccctcca ttgccccccc
     4441 tcgtccggat cagggcacga ggttcacgtt cgtcaggttg aacggcccga gcagcggcga
     4501 ccctgtacgg tccagcat
//
LOCUS       DAORPG010000081         4492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1365_length_4492_cov_3.639621, whole genome shotgun sequence.
ACCESSION   DAORPG010000081 DAORPG010000000
VERSION     DAORPG010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1365_length_4492_cov_3.639621"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /gene="eno"
                     /locus_tag="PLX85_02790"
     CDS             complement(<1..792)
                     /gene="eno"
                     /locus_tag="PLX85_02790"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013555172.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRC62135.1"
                     /translation="MDIEDIRAREILDSRGNPTVEVDVILEDGSMGRAAAPSGASTGA
                     YEAIELRDGDPERYKGRGVLQAVEHVQDQLSDAVLGLDATNQAEVDAALIEADGTPNK
                     ARLGANAILAVSLAVAKAAAESTGQPLYRYLGGADARLLPVPMCNLLNGGAHAAGSTD
                     FQEFMVVPLGATTFSEGLRWVSEIYHSLRGLLHEQGYATTVGDEGGFAPKLAANREAL
                     DLLMRAIELAGRRPGSEIAIALDPATTELYREGQYHLEREGRVVDS"
     gene            933..1190
                     /locus_tag="PLX85_02795"
     CDS             933..1190
                     /locus_tag="PLX85_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62136.1"
                     /translation="MPAADRFEVRLDGERRRKLAEVAAVYGTSASEAVRVIIDQAYDR
                     ITRDRRRLAATALASMAVEDVPDEEELARQLSDARATTGLS"
     gene            1162..1614
                     /locus_tag="PLX85_02800"
     CDS             1162..1614
                     /locus_tag="PLX85_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRC62137.1"
                     /translation="MRVRPLAFLDTNIPIYAAGRPHPLKAPAVRVLTLVAEYPEAFIT
                     DVEVVQELLHRYTALRLWDAGRAVVAQFLVLMEGRVEAVLVDDVRRAAELADEPRDVA
                     ARDLLHAAVMRRLGAEAIVSTDKDFDRIRDVTRLPPAAVDEWSPAFTG"
     gene            complement(1638..2762)
                     /gene="xerD"
                     /locus_tag="PLX85_02805"
     CDS             complement(1638..2762)
                     /gene="xerD"
                     /locus_tag="PLX85_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRC62138.1"
                     /translation="MKRRMLTRREWLKPSHYVSSQAGSIRAEESVSGDIMLGNRVDSD
                     RMTGCILRRPLIGYVKSTVTEGGSAFVVEQRIEAFLHYMSAERGASKNTIDAYRNDLT
                     RFDDFATQRRGTGVAFEAKDIERDLVTDYILWLKQQTYAPATVARKVAAVKSFCAFLL
                     DHGDLTSNPTAAIDSPRAPKPVPRPMTTADVDALLREPQRYDTAEAIRDGAMLELMYA
                     TGMRVTELVSLNVDSMHFLPRPAFVRCLGKGAKERTIPVYEQAVAAVERYFDEARPQL
                     LKDRPQVALFVNRRGERLTRQGFWLILKGYAKSAGIEAHVTPHTLRHSFATHMLRGGA
                     SIRDVQELLGHANVSTTQVYTQLADAHLREVYEQVHPRAH"
     gene            2781..3320
                     /locus_tag="PLX85_02810"
     CDS             2781..3320
                     /locus_tag="PLX85_02810"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRC62139.1"
                     /translation="MRPVRDSDLPDLLRWRQDPEVVEFWDVAPETIEEAREEFLEADI
                     VPLWRFVIEWQGRGVGSVEYSHPYADTDYLWTAGIDIFIGEPAARNRGVGTEAVRTLL
                     QYLFEVKHLHRVTIDPEVGNARAIRSYEKAGFRFDGVLRHNDRFENGRYVDTHFMSII
                     EDEWPAAKNRWEAEVASDD"
     gene            complement(3309..3974)
                     /locus_tag="PLX85_02815"
     CDS             complement(3309..3974)
                     /locus_tag="PLX85_02815"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRC62140.1"
                     /translation="MTAERRDAAIEEMVRLVGRVVRDERVLDAMREVPRDEFVPERVR
                     RYAFDDQALPIGEGQTISQPLIVGMMTEAARLDGHEHVLEVGTGSGYQAAILARLVRD
                     VVSVEIVGSLREQAARTLARLGVTNVTVLPAGEELGAPAFAPYDAILVTAVAPSIPPT
                     LMGQLRVGGRMVLPVGKHADDQELLVVTRTAAGIETTSLGGVRFVPLRGAGGFEEDDE
                     SVV"
     gene            complement(3971..>4492)
                     /locus_tag="PLX85_02820"
     CDS             complement(3971..>4492)
                     /locus_tag="PLX85_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRC62141.1"
                     /translation="LAASRRRRREATVTQAETAEVDAAEEERTAWDIVPDLVIIDGGK
                     GQLGAALDVMRDLELADVPVCGLAKREEEVFVKDVDEPIVLPRDSQALYLIQRVRDEA
                     HRFAITYHRKLRGQSAQRSVLDSIPGVGPRRKRALLRKFGSVRALQEASVEEIATTVG
                     FTRRLAETVKRSI"
BASE COUNT          700 a         1603 c         1431 g          758 t
ORIGIN      
        1 cgagtcgacg acacggccct cccgttcgag gtggtactgg ccctcccggt acagttcggt
       61 ggtcgccggg tcaagcgcga tcgcgatctc gctgcccggt cgccgtccgg ccagctcgat
      121 ggcgcgcatg agcaggtcaa gggcttcacg gttcgccgcc agcttcggag cgaagccgcc
      181 ctcgtcgccc acagtggtcg cgtacccctg ctcgtgcagc aacccgcgca acgagtggta
      241 gatctccgag acccaacgca acccttccga gaaggtcgtg gcgccgaggg gtacgaccat
      301 gaattcctgg aaatcggtgg agcccgccgc gtgcgcacca ccgttgagca ggttgcacat
      361 cggcacgggc agcaggcgcg cgtccgcccc gccgaggtat cggtacaggg gctgcccggt
      421 tgattcggcc gcggccttcg cgacggccag cgacaccgcg aggatcgcgt tcgcgcccag
      481 gcgggccttg ttcggcgtcc cgtccgcctc aatgagcgcc gcgtccacct cggcctggtt
      541 cgtcgcatcg aggcccagca cggcgtcgga gagctgatcc tggacgtgct caacggcctg
      601 cagcacgcca cggcccttgt agcgctccgg gtccccgtcc cgcagttcga tcgcctcgta
      661 cgcgccggtg ctcgcgccgc tcggcgccgc cgcccgcccc atcgacccat cctcgaggat
      721 cacgtcgacc tccacggtcg ggttgccgcg agaatccagg atctcgcgcg ctcggatgtc
      781 ctcgatgtcc atgcgaaatg ctcactttca cgcggcgaca gctatgcacc gatgatcggc
      841 tggcgcgacg cggcggacaa ccttgatggc ccccgttatc cgttctccgt acgaatcgta
      901 ccgtacactg catatcgtac aggaggtgcc gtatgccggc tgccgatcgg ttcgaggtcc
      961 gtctggacgg cgagcgccgg cgtaagctgg ccgaagtcgc ggctgtgtat ggcacgagcg
     1021 cgtccgaggc cgtgcgagtg atcatcgacc aggcgtacga ccgcatcacc cgtgatcggc
     1081 gtcgcctcgc tgccacagcc ctcgcctcga tggccgtcga ggacgtcccg gacgaggagg
     1141 agctggcgcg tcagctcagc gatgcgcgtg cgaccactgg cctttcttga cacgaatatt
     1201 ccgatctacg ccgcggggcg ccctcatcct ctgaaggcgc ccgcggttcg cgtcctcacc
     1261 ctggtcgcgg agtaccccga ggcgttcatc acagacgtcg aggtcgtgca ggagctgctg
     1321 catcgctaca ccgcgctgag gctctgggac gcgggtcgcg ccgtagtggc ccagttcctg
     1381 gtgctcatgg aagggcgcgt cgaggccgtc ctcgtcgacg acgtccgccg cgccgccgag
     1441 ctcgccgatg agcctcgcga tgtcgccgcg cgcgacctcc tgcacgcggc cgttatgcgc
     1501 cgcctgggcg cggaagcgat cgtgagcacc gacaaggact tcgaccgcat tcgagatgtg
     1561 acccgcctgc cgccagcggc ggtagacgag tggagtccgg cgttcaccgg gtaagcggct
     1621 cccccggctg ccctccgtca gtgcgcccgg ggatgcacct gctcgtacac ctcgcggaga
     1681 tgcgcatcgg cgagctgcgt gtacacctgc gtcgtcgaca cgttcgcgtg gccgaggagt
     1741 tcttggacgt cccgaatcga ggcgccacct cgcagcatgt gcgtggcgaa cgaatggcgc
     1801 agcgtatgcg gcgttacgtg cgcctcgatg cccgccgact tcgcgtatcc cttgaggatg
     1861 agccagaacc cttggcgtgt gagccgctca ccgcggcggt tcacaaatag cgcgacctgt
     1921 ggccggtcct tcaacagttg cgggcgggcc tcgtcgaagt aacgctccac cgccgcgacc
     1981 gcctgctcgt acaccgggat tgtgcgctcc ttggctcctt tgccaaggca ccgcacgaac
     2041 gccgggcgcg ggaggaagtg catgctgtcg acgttcagcg acaccagctc cgtcacccgc
     2101 atccccgtcg cgtacatcaa ttcgagcatc gcgccgtcgc ggatggcctc cgccgtgtcg
     2161 taccgctgcg gctcccgcag caatgcatcg acgtccgcgg tcgtcatcgg gcgcgggacc
     2221 ggcttcggcg cgcgcggcga gtcgatcgcc gcggtggggt tcgaggtgag gtcgccgtgg
     2281 tcgagcagga acgcgcagaa cgacttcacc gccgctacct tgcgggccac cgtcgccggc
     2341 gcgtacgtct gctgcttcag ccagaggatg tagtccgtaa cgaggtcgcg ttcgatgtcc
     2401 ttcgcctcga aggcgacgcc cgtcccgcgg cgctgggtcg caaaatcgtc gaaccgggtg
     2461 aggtcgttac ggtacgcgtc gatcgtgttc ttagaagcgc cgcgctccgc cgacatgtaa
     2521 tgtaggaacg cttcgatgcg ttgctccacg acgaacgcgc tcccgccctc agtcactgtc
     2581 gactttacat agccaatcag cggacgccgc agtatacaac ccgtcattct gtcactgtcc
     2641 actcgattac cgagcataat gtcgcctgaa accgactcct cggctcggat cgagcctgcc
     2701 tgcgaggaaa cataatgact cggcttcagc cactcccgcc gggtgagcat ccgcctcttc
     2761 atggtacgca cggcgtctcc ttgcgccccg tccgcgactc ggacctgcct gacctcctcc
     2821 gctggcgtca ggatcccgaa gtcgtggagt tttgggatgt cgcgcccgag accatcgagg
     2881 aagcgcgcga ggagttcctc gaggcggaca tcgtcccgtt atggcgcttc gtcatcgaat
     2941 ggcaggggcg aggcgtcggc agcgtcgagt acagccaccc gtacgctgat accgactacc
     3001 tctggaccgc gggcatcgac atcttcattg gcgagcccgc cgcccggaac cgcggtgtgg
     3061 gcaccgaggc ggtgcgtacg ctgctccagt acctgttcga ggtgaagcac ctgcatcgcg
     3121 tcacaatcga ccctgaggtc ggcaacgccc gtgccatccg ttcctacgag aaggctggtt
     3181 tccgcttcga cggcgtgctg cgacataacg accgcttcga gaacggtcgc tacgtggaca
     3241 ctcacttcat gtccatcatc gaggacgagt ggccggccgc gaagaaccgc tgggaggctg
     3301 aggtcgcctc agacgactga ctcgtcgtcc tcctcgaagc caccggcgcc acgcagcggc
     3361 acgaaccgca ccccgcccaa cgaagtcgtc tcgatgccag cggccgtccg cgtgaccacg
     3421 aggagctctt ggtcgtcggc atgtttgccg acgggcaaca ccatgcgccc accgacgcgg
     3481 agttgcccca tgagcgtcgg gggaatcgag ggcgcgacag ccgtcacgag gatggcgtcg
     3541 tagggcgcga acgcgggcgc gccgagctcc tcacctgccg ggagcaccgt cacattcgtg
     3601 acgccgaggc gtgccagcgt ccgcgcggcc tgctcgcgca acgatcccac gatctccacg
     3661 ctcacgacgt cacgcacgag tcgcgcgaga atcgccgcct ggtaccccga gcccgtcccg
     3721 acctccagca cgtgctcgtg gccgtcgagg cgcgcggcct ccgtcatcat ccccacgatc
     3781 agcggctgcg agatggtctg accctcgccg atcgggagcg cctggtcatc gaaggcgtac
     3841 cgccgcacac gctccggtac aaactcatca cgaggcacct ctcgcatcgc gtcgaggacg
     3901 cgctcgtcgc gcacgacccg gccgacgaga cggaccatct cctcgatggc tgcgtctcgt
     3961 cgctccgccg tcatatcgac cgtttgacgg tctccgcgag acggcgcgtg aagcccaccg
     4021 tcgtggcgat ctcctccacg ctcgcctcct gcagcgcccg cactgagccg aacttccgca
     4081 agagcgcgcg cttacgccgc ggcccgacgc ctggaatcga gtcgaggacg gagcgctgag
     4141 ccgactgccc gcgcagcttg cggtggtacg tgatcgcgaa gcggtgagcc tcgtcccgta
     4201 cgcgctggat caggtacagc gcctgcgagt cgcgaggcag cacgatcggc tcgtcgacgt
     4261 ccttgacgaa cacctcctct tcacgtttcg cgaggccgca caccggaacg tccgccagct
     4321 cgaggtcgcg catgacgtcg agcgccgccc ccagctgccc cttcccgccg tcgatgatga
     4381 cgaggtccgg gacgatgtcc cacgccgtcc gctcctcttc cgccgcgtcc acttcggccg
     4441 tctcggcctg cgtcacggtc gcctcgcgcc gccggcggcg gcttgcggcc ag
//
LOCUS       DAORPG010000082         4429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1420_length_4429_cov_3.310242, whole genome shotgun sequence.
ACCESSION   DAORPG010000082 DAORPG010000000
VERSION     DAORPG010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1420_length_4429_cov_3.310242"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..901
                     /locus_tag="PLX85_02825"
     CDS             131..901
                     /locus_tag="PLX85_02825"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC62142.1"
                     /translation="MLALHSVSKRFHTRRQDTLALDRVTLEVRPREFICIVGPSGCGK
                     STLLNIAAGIDRDYEGQALFEGAPIRGAARERAVVFQEPALFPWLNVRANVEFGLKLR
                     GVPKRQRREVVDRQLALVDLSKFERAYIHELSGGMKQRAQLARALAVEPHVLLMDEPF
                     AALDAQTRDDLQLELQSVWARIGTTVLFVTHNVREAVILGDRVVVMSASPGRIKRIID
                     VPLERPRTPDDHAVADLAAQIRLELQNTAEDLEPRYAI"
     gene            891..1679
                     /locus_tag="PLX85_02830"
     CDS             891..1679
                     /locus_tag="PLX85_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC62143.1"
                     /translation="MPSSRFLPSSELLWKLLRILAFYALLVVAWEAVAKAEIWSPFLF
                     PAPEKVFDALRNNIESGVLFEALRGTMKRLLVGYGISFVIGMALGLFIASWKWADETV
                     GGIVLGLQSLPSIVWLPMAVLWFGLSERGIIFVVLMGSTFSIALSARAGIRALPPLLA
                     RAAGTLGASRLQMYRYVVLPAMIPAMIQGMKQGWSFAWRSLMAAELLLSVGIGHQLQI
                     GRDLNNISMVFAIMLVIVAVGVAVGLLFSRAEAWVHERWGFAAG"
     gene            1872..3653
                     /locus_tag="PLX85_02835"
     CDS             1872..3653
                     /locus_tag="PLX85_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC62144.1"
                     /translation="MSDPIETQEGRWSMNRRALLRGGSLAFAGVAAAALIGCGSDDEK
                     SSGSASSTAPAGTGTAAASNVDQAPGLPYPFNFKEPNKTAKDGGTMTVGVSWDVSTFD
                     QAKSAAGGTITVPNIVADRLIGFKSGVNYDRLKLELKPELATSWERSPDGLIYTFKIR
                     PNVKWQNVAPVNGRAFVAADAAYAYERYRKEGVHTAIWSDVDKIEATDASTLKITMKK
                     PLVDFLNNLAGRYQTIFPKESVDDGSIEKKPIGTGPMIFQDAKAQQQVTLTKNPDYWQ
                     GKVHLDGWVFKIMPDIAARTAAFRAGQLDYGYGLAAKLSEAQNIEKSMTGLQMQITGG
                     ESGGYGFGMNVSNPKFKDERVRRAIALAQDHQTSLQLIYEGLGVALPDQYWTFVFDTQ
                     PDFKTGGLGQWTKASGDPAQAKQLLEAAGAANLTIDATYYTYAGYDATRPEVLTDQFR
                     RAGITLNAKKVEYTEFNSQWVGAKLAEATTSGWGAPGFDADNYFYNQLYSTSPGNRHQ
                     IKDPQIDQWAEQQRVELDPAKRKDLHKKIWDRVWTDQMYRIPQAGGYAFELYQPWLRG
                     FRSGGPRGTSSYFYDWGEQVIDMWLDK"
     gene            3970..>4429
                     /locus_tag="PLX85_02840"
     CDS             3970..>4429
                     /locus_tag="PLX85_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRC62145.1"
                     /translation="MDSVWRSRGMRDFGPDAMRRFRRVERAFLDVTASRGYAEIRTPA
                     IEPIHLFTATGSLSPQLLDRVYSFLDWDGWSGERVVLRPDSTVPAARWYETNWRDASP
                     ARLSYVQPVYRFEPGDAEREQWQCGVELFGLEALEADGELLRLAADFLAAL"
BASE COUNT          733 a         1613 c         1340 g          743 t
ORIGIN      
        1 cgaggacagc gccgatcccg ccgtcgaggc ggccgtcgcc cctcctcctg caccacgggc
       61 gacggcggcc ccgacggtgt cgctcccggc tacaacgacg cccgtttcac ccgtggaggg
      121 cgagcagcct gtgctcgccc tccacagcgt ctccaagcgc ttccacacac gacgccagga
      181 cacgctggca ctcgaccgcg tgaccctcga ggttcgccct cgcgagttca tctgcatcgt
      241 cggcccgtcc gggtgcggca agtccacgct gctgaacatc gccgccggca tcgaccgcga
      301 ctacgaaggc caggccctct tcgagggcgc ccccatccgc ggcgccgcac gcgagcgtgc
      361 cgtggtgttc caggagcccg cgctgttccc gtggctgaac gtccgcgcca atgtcgagtt
      421 cggactcaag ctgcgcggcg tcccgaagcg ccagcgccgc gaggtcgtcg accgtcagct
      481 cgccctcgtc gacctctcga agttcgagcg cgcgtacatc catgagcttt cgggcggcat
      541 gaaacaacgg gcccagctcg cccgtgcgct cgcggtggag ccgcatgtcc tactgatgga
      601 cgagccgttc gcggcgctcg acgcccagac gcgcgacgac ctccagctcg agctgcagtc
      661 cgtgtgggca cgcattggca ccaccgtcct cttcgtcacc cacaacgtgc gcgaggcggt
      721 gatcctcggg gaccgcgtcg tggtcatgtc cgcgagcccg gggcgcatca agcgcatcat
      781 cgatgttccg ctcgaacgcc cacgtacccc ggacgaccac gccgtcgccg acctcgccgc
      841 ccagatccgt ctcgagctcc agaacaccgc cgaggacctg gagccccgct atgccatcta
      901 gtcgcttcct gccgtcgtcc gaattgctgt ggaagctgct gcgcatcctc gcgttctatg
      961 cgctgctggt cgtggcctgg gaagccgtcg cgaaagccga gatctggtcg cccttcctct
     1021 tcccggcccc cgagaaagtc ttcgatgccc tgcggaacaa catcgaaagc ggtgtcctct
     1081 tcgaggcact gcgagggacg atgaagcgac tgttggttgg ctatggaatc tcgtttgtga
     1141 ttggcatggc cctcggcttg ttcatcgctt cctggaagtg ggccgacgag acggtcggcg
     1201 gcatcgtcct cggcctgcag tcgctgccga gcattgtctg gttgcccatg gccgtcctct
     1261 ggttcggcct cagcgagcgc ggcatcatct tcgtcgtcct catgggctcc acgttctcta
     1321 tcgccctctc cgcacgcgcg ggcatccgcg ccctcccgcc gctgctcgcg cgcgctgccg
     1381 gcaccctggg agcctcgcgt ttgcagatgt accgctacgt cgtgctgccc gcgatgattc
     1441 ccgccatgat tcaggggatg aagcagggct ggtcattcgc ctggcgctcc ctcatggcgg
     1501 ccgagctcct cctcagcgtc ggcatcgggc accagctcca gatcggccgc gacctgaaca
     1561 acatcagcat ggtcttcgcg atcatgctgg tcatcgtcgc cgtgggcgtc gccgtcggcc
     1621 tcctcttcag ccgggccgag gcttgggtgc acgaacgctg gggcttcgct gccgggtgac
     1681 cctccgccga ggaggccggc ccgcccagac gcgcggtgac catgacattt ctgttcgtaa
     1741 tcaccgcggt gacgcgccga tttcattccc ttcgtaatcg aaaatggggc cactttcgat
     1801 cgttatttga gcgtatcccc aaccctctat tacgttctct gcacggcaaa cgtatccaga
     1861 ggggagacta catgtcagat ccaatcgaga cgcaggaggg gcgctggtcg atgaaccgcc
     1921 gtgccctcct ccgaggtggt tcgctcgcct tcgcgggcgt cgccgccgcc gccctcatcg
     1981 gctgtggcag cgacgacgag aagtccagcg gcagcgcctc gagcaccgcg ccggccggga
     2041 ccggcaccgc tgccgcctcc aatgtcgacc aggctccggg gctgccttac cccttcaact
     2101 tcaaggagcc gaacaagacc gccaaggacg gcggcaccat gaccgtgggc gtcagctggg
     2161 acgtctcgac gttcgaccag gccaagtccg ccgccggcgg caccatcacg gtcccgaaca
     2221 tcgttgccga ccggctcatc ggctttaaga gcggcgtcaa ctacgaccgc ctgaagcttg
     2281 agctcaagcc ggagctcgcg acctcctggg agcgcagccc ggacggcctc atctacacct
     2341 tcaagattcg cccgaacgtg aagtggcaga acgtcgcccc ggtcaacggc cgtgctttcg
     2401 ttgccgccga cgccgcttac gcgtacgagc gctaccgcaa ggaaggcgtc cacaccgcca
     2461 tttggtcgga cgtcgacaag atcgaggcca ccgacgcctc caccctgaag atcacgatga
     2521 agaagccgct cgtcgacttc ctcaacaacc tcgccggccg ctaccagacc atcttcccca
     2581 aggagtccgt cgacgacggc tccatcgaga agaagcccat cggcaccggc ccgatgatct
     2641 tccaggacgc caaggcgcag cagcaggtca ccctgaccaa gaaccctgac tactggcagg
     2701 gcaaggtgca cctcgacggc tgggtcttca agatcatgcc ggacatcgca gcccgcaccg
     2761 ccgccttccg cgccggtcag ctcgactacg ggtacggtct cgccgcgaag ctcagcgagg
     2821 cgcagaacat cgagaagtcg atgaccggcc tgcagatgca gatcaccggt ggtgagtccg
     2881 gcggctacgg cttcggcatg aacgtgtcga accccaagtt caaggacgag cgcgtgcgcc
     2941 gcgctatcgc cctggcgcag gaccaccaga cgtcgctcca gctcatctac gagggcctcg
     3001 gcgttgcgct gcccgaccag tactggacct tcgtcttcga cacccagccc gacttcaaga
     3061 caggcggcct cggccagtgg accaaggcct cgggcgaccc cgcccaggcg aagcagctcc
     3121 ttgaggctgc cggtgccgcg aacctgacga tcgacgccac gtactacacg tacgccggct
     3181 acgacgccac ccggcccgag gtgctcaccg accagttccg ccgcgcgggt atcaccctca
     3241 acgccaagaa ggtcgagtac accgagttca actcgcagtg ggtcggcgcc aagctcgccg
     3301 aggccaccac gagtggctgg ggcgccccgg gcttcgacgc ggacaactac ttctacaacc
     3361 agctgtactc cacgtcgccc ggcaaccgcc accagatcaa ggacccgcag atcgaccagt
     3421 gggccgagca gcagcgggtc gagctcgacc cggccaagcg caaggatctc cacaagaaga
     3481 tctgggaccg cgtctggacc gaccagatgt accgcatccc ccaggcgggt gggtacgcct
     3541 tcgagctcta ccagccgtgg ctccgcggct tccgctccgg cggtccccgc ggtacctcca
     3601 gctacttcta cgactggggc gagcaggtca tcgacatgtg gctcgacaag tagtcgagtc
     3661 gcgctagcga ccggtgtggt ccgcctcgtg ccgcttgatc gtggcacgag gcggacctac
     3721 cgttttttcg aggagtgttc gatgacgcac gccgcctccc gcacccgcgc ccatgcctgc
     3781 tcggccatgc cgctgcgagc tcgtctgcgt cgttagactc ccgtcccgaa cgctcgatga
     3841 cacgccgcgg cagctcccgc ggcgttttgc tgtaacccac ctgcaccgga tcgaggaacg
     3901 cgaccgttga gcaccgtaga atcccccgag aacacatcgg ctctcgccag ggacgcgcga
     3961 ggagacagca tggacagtgt ttggcgtagc cgcggcatgc gtgacttcgg cccggacgcc
     4021 atgcgccgct tccgccgcgt tgagcgcgcc ttcctcgatg tcaccgcgag ccgcggctat
     4081 gccgagatcc gcacgcccgc tatcgaaccg atccatctgt tcacggccac cgggtcgctc
     4141 tcaccgcagc tcctcgaccg cgtgtactca ttcctcgact gggacggctg gagtggcgaa
     4201 cgcgtcgtcc tgcggcccga cagcaccgtc cccgccgccc gctggtatga gacgaattgg
     4261 cgcgatgcct cgccggcccg tctctcctac gtccagcccg tctaccgctt cgagcccgga
     4321 gacgccgagc gcgagcagtg gcaatgcggc gtcgagctgt tcggactcga ggcgctcgag
     4381 gcggacggcg aactgttgcg gcttgccgcc gacttcctcg ccgcgctcg
//
LOCUS       DAORPG010000083         4412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1430_length_4412_cov_3.309387, whole genome shotgun sequence.
ACCESSION   DAORPG010000083 DAORPG010000000
VERSION     DAORPG010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1430_length_4412_cov_3.309387"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLX85_02845"
     CDS             <1..184
                     /locus_tag="PLX85_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62146.1"
                     /translation="DAIGALGAASPAELQQVLEALDVRRRLEFALPLLEQRLKAVHRE
                     AAAAAAARWAAPGSAN"
     gene            243..334
                     /locus_tag="PLX85_02850"
     tRNA            243..334
                     /locus_tag="PLX85_02850"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:277..279,aa:Ser,seq:gct)
     gene            353..1648
                     /gene="miaB"
                     /locus_tag="PLX85_02855"
     CDS             353..1648
                     /gene="miaB"
                     /locus_tag="PLX85_02855"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRC62147.1"
                     /translation="MPTYHIWTLGCQMNVADSLKLASGLDRIGWSESPDEGSADLFVI
                     NTCSVRQHAEDRAYGRLHQLRQQRQRGEQFQIAVMGCMVGPKTDDLQRRFPWVDVWAR
                     PQEYDPILTAAGVARDASEGEFWTETYGRPSGPTAYVPVVHGCDKFCTYCIVPLRRGR
                     ERSRPAAEVLDEVRYLAAHGVREVTLLGQTVEAYGHDLDEGADLATLFEGIEAIDGIV
                     RTRFLTSYPKDMTDRIIDAVADLPKVCEHFNIPVQSGDDAMLERMRRGYTVAEYEERV
                     EYIRKRMPHASLATDIIVGCPSETDEEFANTVALLERIQFDVIHVAAYSPRPGTFAYR
                     KQVDDVPREVKRERLHVIEELHARSSSAINRQALGRQVEVLVEREQDGRSTGRTRGGQ
                     LVHFAGTGLVGQLVDVAVDEVTAWSLHGRLAGDLTLAVV"
     gene            1665..2300
                     /locus_tag="PLX85_02860"
     CDS             1665..2300
                     /locus_tag="PLX85_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRC62148.1"
                     /translation="MTPPVVATIVVIFAMIDGSLQVLLVHRSAEPERGRWALPGGRWD
                     GSMTLEEAAGRKLVEETGASDLYLEQLFTASNLDATQPSVAVAYFALVEQRRVRLRSS
                     EEWPPAWHRVDALPALAFRNNEVIAQAVTRVRAKLEYTNLAYGLLPERFTLRQLQTTY
                     EAILGERLDRRNFRKRMLSSGLIAPTGELERAGAHRPASLYRFTSREPVFL"
     gene            complement(2302..2787)
                     /locus_tag="PLX85_02865"
     CDS             complement(2302..2787)
                     /locus_tag="PLX85_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRC62149.1"
                     /translation="MEQNEPSALIKETAVLFGRSTRLVDPMRLRMWEEQGITFPQLRI
                     LFRVRMHPDIDLRTLADGLAISPSAASQQVDKLVERGLLWRTEDPQDRRRLRLQLTPQ
                     AEEAVGEYSRTVTEYISAILARLSQKDLKELHRLLGTLEELNSPPPPLPSSVSGRAVA
                     S"
     gene            2876..3826
                     /locus_tag="PLX85_02870"
     CDS             2876..3826
                     /locus_tag="PLX85_02870"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC62150.1"
                     /translation="MQTPVEAVEAPQAVGEGARRLRLPPAFASLRYRNYRLLWFGQLV
                     SSSGDWMDQVALNWLVVSQLYDGREAALPVAALNACRLVPILVFTPIGGVLADRLERR
                     RLMFTTQAAAMVMAFVLATLVATGLVEFWMVLAIALGRGVMLSFNMPARQSLISELVP
                     REALTNAVALNSATLNLTRVAGPLIAAIFFLTPFGIAGAFYFNGLTFLAVLAGLAIME
                     IPRQPARARQGMLSELAGGFAYINRSVVLRTLVILATVPMVFGMPYQTMSAVVSRDVL
                     GTGGEGFGLLTAATGVGAVCGALTVASLASFTWLERLRSR"
     gene            4211..>4412
                     /locus_tag="PLX85_02875"
     CDS             4211..>4412
                     /locus_tag="PLX85_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62151.1"
                     /translation="MICAVEREDGAIWASGYGCQGKAIASAAFGRLSGDLTERAEHPT
                     FRGIVAAEGGQVQEHHADQVSES"
BASE COUNT          647 a         1463 c         1583 g          719 t
ORIGIN      
        1 cgacgcgatc ggggcgctgg gcgcggcgag ccccgccgag ctgcaacagg tccttgaagc
       61 cctcgatgtc cgccgccgcc tcgagttcgc gctgccgttg ctcgagcagc ggctgaaggc
      121 ggtgcaccgg gaggcggctg cggcagcggc agcgcgatgg gcagcgccgg gaagcgccaa
      181 ctaaggcgtc gattggctcg ccttgcccgg cacggcggcg caacatagga tcgatgtccg
      241 ctggaggggt cgcatagtgg cctagtgcgg gcgcctgcta agcgcttact cggggtttcc
      301 cgggtcgtgg gttcgaatcc caccccctcc gccagcgtcc gaggagtcct cgatgcccac
      361 gtaccacatc tggacgctcg gctgccagat gaacgtcgcc gactcgctca agctcgcctc
      421 cggcctcgac cggatcggct ggagcgagag cccggacgag ggctcggccg acctcttcgt
      481 catcaacacc tgctccgtgc gccagcacgc tgaggaccgc gcgtacggcc ggctgcacca
      541 gctgcggcag cagcggcaac gtggcgagca attccagatc gcggtcatgg ggtgcatggt
      601 cggcccgaag accgacgacc tgcagcgccg cttcccgtgg gtcgacgtgt gggcgcgccc
      661 gcaggagtac gacccgatcc tgactgccgc cggggtggct cgcgacgcct cggaaggtga
      721 gttctggaca gagacgtacg ggcggccgtc cggcccgacc gcctacgtgc cggtcgtcca
      781 tggctgcgac aaattctgca cctactgcat cgtgccgctc cgtcgcggcc gcgaacgctc
      841 gcggcccgcc gccgaggtgc tcgacgaggt gcgctacctc gccgcgcacg gcgtgcgcga
      901 ggtcacactg ctcgggcaga cggtcgaggc gtacgggcac gacctggatg agggcgccga
      961 cctcgcgacg ttgttcgagg ggatcgaggc gatcgacggc atcgtgcgga cgcggttcct
     1021 cacctcgtat ccgaaggaca tgacggaccg catcatcgat gccgtcgcgg acctgcccaa
     1081 ggtctgtgag cacttcaaca tcccggtgca gtctggcgac gacgcgatgc tcgagcgtat
     1141 gcgccgcggc tacacggtcg cggagtacga ggagcgcgtc gagtacatcc gcaagcggat
     1201 gccgcacgcc tcgctggcga cggacatcat cgtcgggtgc ccaagcgaga cagatgagga
     1261 gttcgcgaac accgtcgcgc tgctcgagcg gatccagttc gacgtgatcc acgtggcggc
     1321 gtactcaccc cggccgggca ctttcgccta ccgcaagcag gtcgacgatg tgccacgcga
     1381 ggtgaagcgc gagcggctgc acgtcatcga ggagctgcac gcgcgttcct caagcgcgat
     1441 caaccggcag gccctcggcc gccaggtgga agtgctcgtg gagcgcgagc aggacggccg
     1501 ctcgaccggc cgcacgcgtg gggggcagct cgtgcacttc gcggggacgg gtctcgtggg
     1561 gcagctcgtc gacgtggccg tcgacgaggt cacagcctgg tctctgcacg gccggctggc
     1621 gggcgacctc acgctggcgg tcgtctagcc ggggcctcca cctcgtgacc cctcccgtcg
     1681 tcgcgaccat cgtcgtcatc ttcgcgatga tcgacggctc attgcaggtg ctgctggtgc
     1741 atcggagcgc cgagccggag cgcggacggt gggcgctgcc gggcggacgc tgggacggca
     1801 gcatgaccct tgaggaggcg gcggggcgca agctcgtcga ggagacgggc gcgagcgacc
     1861 tctacctcga gcagctgttc accgcttcga acctggacgc cacgcagccg agcgtcgcgg
     1921 tcgcctactt cgcgctggtg gaacagcgac gcgtccggct gcgctcctcg gaggagtggc
     1981 cgccggcatg gcaccgggtg gacgccctgc cggcactcgc gtttcgcaac aacgaggtga
     2041 tcgcgcaggc cgtgacgcgg gtgcgggcga agctggagta cacgaacctc gcctacgggc
     2101 tgctcccgga gcgctttacg ctccgtcagc tgcagacgac gtacgaggcg atcctcggcg
     2161 agcgcctcga ccggcggaac ttccgcaaac ggatgctctc atcgggactg attgcgccga
     2221 cgggcgagct ggaacgggcc ggcgcgcacc ggcccgcgtc gttataccgc ttcacgagcc
     2281 gggagccggt cttcctctag ttcagctcgc gacggcgcgg ccggacacgc tcgaggggag
     2341 cggaggcggc gggctgttca actcctcgag cgtgccgagc aagcggtgca gctccttgag
     2401 gtccttctgg ctgaggcggg cgaggatcgc ggagatgtac tcggtcacgg tacgcgagta
     2461 ctcgcccacg gcttcctcgg cctgcggagt gagctgcagg cgaagacgcc ggcggtcctg
     2521 ggggtcctcg gtgcgccaga gcaggccgcg ctcgacgagc ttgtcgacct gctggctggc
     2581 ggcggacgga ctgatcgcga gcccgtccgc gagcgtgcgg aggtcgatgt ccgggtgcat
     2641 gcgcacccgg aacaggatcc gcagctgcgg gaatgtgatg ccctgctcct cccacatgcg
     2701 caggcgcatg gggtcgacga ggcgggtcga gcgaccgaag aggacggccg tttccttgat
     2761 gagggcgctt ggttcgttct gttccatcgc cgcgcatggt accagcgaac cgcccgagtt
     2821 cctagcactc gaccagatag tttagtattt cttcagtagc taaacaggcg ggacgatgca
     2881 gactccagtc gaggccgtcg aggcacctca ggcagtaggc gagggcgcgc ggcgcctgcg
     2941 cctgccgccg gcctttgcct cgctgcgcta tcgcaattac cggctgctgt ggttcggtca
     3001 gctcgtttcg agttcgggcg actggatgga ccaggtcgcg ctgaactggc tggtcgtgag
     3061 ccagctgtac gacggccgcg aggcggccct gcccgtcgcc gcactcaacg cctgccgcct
     3121 ggtgccgatc ctcgtgttca cgccgattgg cggcgtgctg gcggaccgcc tcgagcggcg
     3181 gcggttgatg ttcacgacgc aggccgccgc gatggtgatg gcgttcgtgc tggcgaccct
     3241 ggtggcgacc ggactcgtcg agttctggat ggtgctcgcg atcgccctcg ggcggggcgt
     3301 catgctctcc ttcaacatgc cggcgcggca gtcgctgatc tcggagctcg tgcctcgcga
     3361 ggcgctgacg aacgcggtcg cgctcaactc ggcgacgctg aacctgacgc gcgtggcggg
     3421 cccgctgatt gcggcgatct tcttcctcac gccgttcggc atcgccggtg cgttctactt
     3481 caacggcctg accttcctcg cggtgctcgc cggcctcgca atcatggaga tcccgaggca
     3541 acctgcccgc gctcgccagg ggatgctcag cgagctggcg ggcgggttcg cctacatcaa
     3601 ccgctcggtc gtgctgcgca cgctggtcat cctcgccacg gtcccgatgg tattcggcat
     3661 gccgtaccag acgatgtccg cggtggtgtc gcgcgatgtc ctcgggaccg gcggcgaggg
     3721 gttcggtctg ctcacggcgg ccacgggggt gggcgccgtc tgcggcgcgc tcaccgtggc
     3781 gtcgctcgcg tccttcacct ggctcgagcg gttgcgctcg aggtaggccg ccgcgacggc
     3841 attggcgatc ttggcggcga gatgcggatc ggccgaggac atatcgatgg tgatcagccg
     3901 ggtcttgccc acctgactga cgcgtagccg ctgctcgatg gcattgagaa cgcgctcgcc
     3961 ctgctcgagg gacgccggat ccccgatcaa gcgcgtcagg atgctgccaa tgagcccgcg
     4021 gtcctgcagg acgccgttga actcggggac ccgctcgagc tgcaggtcgg cggcgacctt
     4081 ggcgagcaga tcgctggagt ggatgacctc gacctggctc gcgacctcgc catcctcgat
     4141 cttgggagct tcagaggtgg gccccgcatc gcccttcggt ctcaggtagg ccgagggggc
     4201 gggctccagg atgatctgcg ccgtcgagcg ggaggatggc gccatctggg ccagcggcta
     4261 tggctgccag ggcaaggcca tcgcctcggc ggccttcggt cgcctgagtg gcgatctgac
     4321 cgagcgcgcc gaacacccga cgttccgcgg gatcgtggcg gccgaaggcg gtcaggttca
     4381 ggagcaccac gccgatcagg taagcgaaag cg
//
LOCUS       DAORPG010000084         4407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1433_length_4407_cov_3.453585, whole genome shotgun sequence.
ACCESSION   DAORPG010000084 DAORPG010000000
VERSION     DAORPG010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1433_length_4407_cov_3.453585"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..91)
                     /locus_tag="PLX85_02880"
     CDS             complement(<1..91)
                     /locus_tag="PLX85_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRC62152.1"
                     /translation="MQNDALDAAFAALADPTRRAILARLAKGSA"
     gene            complement(216..1646)
                     /locus_tag="PLX85_02885"
     CDS             complement(216..1646)
                     /locus_tag="PLX85_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HRC62153.1"
                     /translation="MSNRYLLFGIVSLGMLLASMQGSMIGVAFTDIGEGLHAPDRWVG
                     WVIAIFTLAQAVSMPTAGKLSDELGRRTVFVSGVSIFLAASVVCAASPNVYVLIAARA
                     VQGLAGGSLLPSAYGVIGDAFDAESRARAIGMLSSVFPIGGILGPNLGGVIVDHLGWR
                     WTFLVNLPLGVVVVLTALLLMPGATNRQRRPIDFGGVALLSVTVTGIIYALTELSQTK
                     GGAHPLVVGSSLAVALAAGFLFLRHEARTAEPILELQLLKRREFAFMNTLNFFYGLVI
                     FGLFSFIPVYAQDAYNMSRSASGLLLTPRAIAMIVASSLGALLLPRTGYRKPIVVGML
                     GMAVTLLIMSRGIHDPSVGGVEVSNFAYLAALAGMSGFFFGVSGPAANNAAIELAPDR
                     IAAITGLRGMFRSVGGSIGTSLVVLVMSHAATREQGLEEAFIGLAVAAALVSLLVVGI
                     PDRAGTRVSVRSAPAGAASPGAAGDS"
     gene            complement(1822..2961)
                     /locus_tag="PLX85_02890"
     CDS             complement(1822..2961)
                     /locus_tag="PLX85_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HRC62154.1"
                     /translation="MPDTSYAWQEGWESAWQPLMDLVGEDFSEGETQPAIDVVEKTSI
                     RRYLEPLEFGCPLHFDEAAAKASGYKGILAPYSGISQTWTDQGQWNPGDPTNYPNADP
                     NFNSVRRQAAAAGRPVPQPDTTAGFATDIEIEYFEPVCVGDHLTYSGRKLISVVPRET
                     SVGRGAFTVWQREVHNQRGELVAQLRNGGYGYVPKPRDPGAAASTSAPAAAGPSGPIM
                     DPAPAPHAAGITNREVDWGQQRYWEDVNEGDEVPPVIFTMTVFRLVVEAGANRDFNQI
                     HHNTPVTQAQGAPDMYANNVFIQGMWERCVREYIGLQGRFKKTGPFRMRIFNNVGETV
                     VTKGTVKRKWVEGGEHLVELEIVSEHSRGISVGPGPVVVTLPSRG"
     gene            3099..3596
                     /gene="purE"
                     /locus_tag="PLX85_02895"
     CDS             3099..3596
                     /gene="purE"
                     /locus_tag="PLX85_02895"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016328988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRC62155.1"
                     /translation="MEQPLAGIIMGSKSDWETMSHAAETLDSLGVPYEVRVVSAHRTP
                     DLLFEYAASAEGRGLRVIIAGAGGAAHLPGMTAAKTTLPVLGVPVESRALKGMDSLLS
                     IVQMPAGIPVATLAIGRAGAVNAALLAAAILGLTHPEIKLAVHRYRAQQTQSVLEQPD
                     PRADG"
     gene            3596..>4407
                     /locus_tag="PLX85_02900"
     CDS             3596..>4407
                     /locus_tag="PLX85_02900"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HRC62156.1"
                     /translation="MVGVLGGGQLGQMLALAGIPLGLRFRFFEPAEESCVDGIGERIR
                     AGYDDLDALARFADGLDVVTYEFENVPVEAVEFLGTLVPHVYPPVGALAVAQDRLHEK
                     TFLERQGVPCPPFAPVNSDAELREAAGRIGLPGVLKTRRFGYDGKGQVVLRSEADLEG
                     AWGVIGGAPAILERFVPFEREASILAVRGRDGAIACYPLVENRHRDGILRLSVAPAPH
                     LEAGLQAQAEAHARAVIEALDYVGVLAIEFFVEGGRLLANEMAPRVHNSGHWT"
BASE COUNT          694 a         1317 c         1755 g          641 t
ORIGIN      
        1 tggctgagcc cttggcaagg cgggcgagga tcgcgcgcct cgtcgggtcg gcgagcgcag
       61 cgaatgcggc atcgagcgcg tcgttttgca tcgccatata cgcgccgacc ggccagggtc
      121 tgcggcttcg gtggacggct ggatgacgag agagggggcc ggatggcccc ctctctgttg
      181 cgccgcgaag agcggtaacc cctggggggc gggggttagc tatcgccggc ggcgccggga
      241 gaggccgcgc ccgcgggggc ggagcggacc gagacgcgag tgccagcgcg gtcggggata
      301 ccgaccacga gcagcgagac gagggccgcc gcgactgcca gcccgatgaa ggcctcctcg
      361 aggccctgtt cgcgcgtggc ggcgtggctc atgacgagca cgacgaggga ggtgccgatg
      421 gagccgccga cggagcggaa catgccgcgc aggccggtaa tggccgcgat gcggtcgggc
      481 gccagctcga tggcagcgtt attggcggcc gggccggaga cgccgaagaa gaagccggac
      541 atgcccgcga gcgccgcgag gtaggcgaag ttggagacct cgacaccgcc gaccgagggg
      601 tcgtggatgc cgcgcgacat gatcagcagg gtgacggcca tgccgagcat gccgacgacg
      661 atcggcttgc ggtagcccgt cctcggcagc agaagcgcgc cgagcgagga cgcgacgatc
      721 atcgcgatgg cgcgcggggt gaggaggagg ccgctcgcgc tgcgagacat gttgtatgcg
      781 tcctgcgcgt acacggggat gaaggagaag aggccgaaaa tgacgaggcc gtagaagaag
      841 ttcagcgtgt tcatgaacgc gaactcgcgg cgcttgagga gctgcagctc gaggatcggc
      901 tcggcggtcc tcgcctcgtg gcggaggaag aggaagccgg cggcaagggc gacggcgagc
      961 gaggaaccga cgacgagcgg gtgtgcgccg cccttcgtct gcgacagctc ggtcagggcg
     1021 tagatgatgc cggtgacggt cacggagagc agcgcgacgc ctccgaagtc gatcggccgg
     1081 cgctggcggt tcgtggcacc gggcatgagc aacagcgcgg tgaggacgac cacgacgccg
     1141 agcggcaggt tgacgaggaa ggtccagcgc cagccgaggt ggtcaacgat cacgccgccc
     1201 aggttggggc cgaggatgcc cccgatgggg aacaccgagg agagcatacc gatagcccga
     1261 gcgcggctct cggcgtcgaa ggcgtcgccg atcacgccgt aggcggaggg cagcaggctg
     1321 ccgccggcga ggccctggac agccctggcg gcgatcagga cgtagacgtt gggagaggcg
     1381 gcgcagacga cggaagcggc caggaagatg ctcacgccgc tgacgaagac ggtgcggcgt
     1441 ccgagctcgt cgctgagttt gccggcggtc ggcatggaga cggcctgcgc gagggtgaag
     1501 atggcgatga cccaaccgac ccagcggtcg ggggcgtgga ggccctcgcc gatgtccgtg
     1561 aaggcgacgc cgatcatcga gccctgcatc gaggcgagca gcatgccgag gctgacgatg
     1621 ccgaagagga ggtagcggtt gctcatgcgt gcctcgatgg gtgcgagcgt gcgcccgctg
     1681 gcgtagagag aatatacgcc tgactgaact attcagcaca ctgcaaccgg cgaggagcgg
     1741 cgaacgactt cggcgttggg ggaagagaag aggcggactc acggcccgcc tcttcgcgta
     1801 cgtaacggct ggccggcgga gctagccgcg tgaaggcagt gtgacgacga cggggccggg
     1861 gccgaccgag atgccgcgtg agtgctccga gacgatctcg agctcgacga ggtgctcgcc
     1921 gccttcgacc cacttgcgct tcacggtgcc cttcgtgacg acggtctcgc cgacgttgtt
     1981 gaagatgcgc atgcggaagg ggccggtctt cttgaagcgg ccctggaggc cgatgtactc
     2041 gcggacgcag cgctcccaca tgccctggat gaagacgttg ttggcgtaca tgtccggggc
     2101 gccctgggcc tgggtaaccg gggtgttgtg gtggatctgg ttgaagtcgc ggttggcgcc
     2161 ggcctccacc acgaggcgga agacggtcat tgtgaagatg accggaggca cctcgtcgcc
     2221 ttcgttgacg tcctcccagt agcgctgctg gccccagtcg acttcgcggt tcgtgatgcc
     2281 ggcggcgtgc ggggcaggcg ccggatccat gatcggaccc gaggggcccg cggcggcggg
     2341 agcgctcgtc gaggcagcgg cgccggggtc gcggggcttc gggacgtagc cgtagccgcc
     2401 attccggagc tgggccacga gctcgcctcg ctggttgtgg acctcgcgct gccagacggt
     2461 gaaggcgccg cggccgacgc tggtctcgcg cgggacgacg gagatcagct tgcgaccgct
     2521 gtaggtgagg tggtcgccga cgcagacggg ctcgaagtac tcgatctcga tgtccgtggc
     2581 gaagccggcg gtggtgtcgg gctggggtac ggggcggccg gccgctgcgg cctggcggcg
     2641 gacgctgttg aagttcgggt cggcgttcgg gtagttcgtc gggtcgccgg gattccactg
     2701 gccctggtca gtccaggtct gggagatgcc gctgtacggg gccaggatgc ccttgtagcc
     2761 gctggccttc gcggcggctt catcgaagtg cagcgggcag ccgaactcga gcggctcgag
     2821 atagcggcgg atcgaggtct tctcgacgac gtcgatggcg ggctgggtct cgccctcgga
     2881 gaagtcctcg ccgacgaggt ccatcagcgg ctgccaggcg gactcccagc cctcctgcca
     2941 ggcgtacgaa gtatcgggca ttactctctc tcccctcgaa acgtgcttgt gcgggcattt
     3001 cacccgcgcg cgggtggcgc gtcaatgggc gcggagcggc cggcctcggt aagatggggc
     3061 ctcggcgaac acgggccatt tcaaggtgtg gagacggcat ggagcagccc ctggccggca
     3121 tcatcatggg gtcgaagtcg gactgggaga cgatgtcgca cgcggcggag acgctggaca
     3181 gcctcggcgt gccgtacgag gtgcgcgtgg tgtcggcgca ccggacgccg gacctgctgt
     3241 tcgaatacgc ggcctcggcc gaggggcgcg ggctgcgggt gatcatcgcg ggggcggggg
     3301 gcgcggcgca cctgcccggc atgacggccg caaagacgac gctgccagtc ctgggcgtgc
     3361 cggtggagtc gcgggcgctg aaggggatgg actccctgct ctcgatcgtc cagatgccgg
     3421 cggggattcc ggtggcgacg ctggcgatcg ggcgggcggg agcggtcaac gcggctctgc
     3481 tggcggcggc gatcctcggg ctcacgcacc cggagatcaa gctcgcggtg caccggtacc
     3541 gagcgcagca gacgcagagc gtgctcgagc agccggaccc gcgagccgac gggtgatcgt
     3601 cggcgtcctc ggcgggggcc agctggggca gatgctggcg ctcgcgggca ttccgctggg
     3661 gctgcggttc cgcttctttg agccggccga ggagtcatgc gtcgacggca tcggcgaacg
     3721 gatacgggcg gggtacgacg acctcgatgc gctggcgcgg ttcgccgacg gcctcgatgt
     3781 cgtgacgtat gagttcgaga acgtgccggt cgaggcagtc gagttcctcg ggacgctcgt
     3841 cccgcacgtg tatccgccgg tgggcgcgct ggccgtcgcg caggaccgac tgcacgaaaa
     3901 gacgttcctc gagcgacagg gggtgccgtg cccaccgttc gcgccggtga actcggacgc
     3961 agagctgcgc gaggcggcgg gacgcatcgg gctaccggga gtactgaaga cgcggcggtt
     4021 cgggtacgac ggcaagggcc aggtggtgct gcgctcggag gcggacctcg agggggcatg
     4081 gggtgtcatc ggcggcgcgc cggcgatcct cgagcgattc gtgccgttcg agcgggaggc
     4141 gtcgatcctc gcggtgcgag ggcgggacgg cgccattgcc tgttacccac tggtggagaa
     4201 caggcatcgc gatggcatcc tgcgactgtc ggtggcgccc gcgccgcacc tcgaggcggg
     4261 gctgcaggcg caggctgagg cgcacgcgcg ggcggtgatc gaggcgctgg actacgtcgg
     4321 cgtgctggcg atcgagttct tcgtcgaagg cggacggctg ctcgcgaatg agatggcgcc
     4381 gcgagtgcac aactcggggc actggac
//
LOCUS       DAORPG010000085         4398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1441_length_4398_cov_3.678333, whole genome shotgun sequence.
ACCESSION   DAORPG010000085 DAORPG010000000
VERSION     DAORPG010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1441_length_4398_cov_3.678333"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PLX85_02905"
     CDS             complement(<1..349)
                     /locus_tag="PLX85_02905"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012408573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC62157.1"
                     /translation="MNEARRGGEYERWLGGRDLHSAVIRWVMSAPGQWAVNSPLMKLP
                     ENLALRREQRWLDVGCGRGALLRLVDQRVGFDTPPTGVDFSMEALRLARRDARKHGHA
                     VRLACGTATTLPFR"
     gene            complement(583..1047)
                     /locus_tag="PLX85_02910"
     CDS             complement(583..1047)
                     /locus_tag="PLX85_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62158.1"
                     /translation="MGRSAIEPAGGRGLFVEFDTDEPCPFGFLGDPTVVLFFISWAFS
                     MRFGGMHELAQAAMHMQRRHKVDLKPLLTYADRDAESEDDRRELERAWQEATPLAASA
                     RAVAMALESDDAELRRLTAGYEELAPRLRELAAICDWAASRGAGVRLSFWLE"
     gene            complement(1064..3001)
                     /gene="gyrB"
                     /locus_tag="PLX85_02915"
     CDS             complement(1064..3001)
                     /gene="gyrB"
                     /locus_tag="PLX85_02915"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRC62159.1"
                     /translation="MTTTARRADGTNAQNAEYTAANIQVLEGLEAVRRRPGMYIGSTD
                     QRGLHHLIYEIVDNAVDEAMAGFCDRVEVLIQPDGSVSIQDNGRGIPVDRHEKTGLSA
                     LETVLTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSEELTAEVAKDGALYRQSYQRG
                     APTGSLEKVRGEKPEQNGTRISFKPDSEIFETLDYDFATLAARFREMAYLTKGLWIQF
                     IDLRGDGQEQNFYFDSGIEAYVRHVNRGRNPLHADPIVIQGERDNISIEVALQYNGSF
                     SESVHSFANCINTIDGGSHMTGFRSALTRALNDYARRAKLLKDDDPNLSGDDVREGLS
                     AVISVKLPEPQFEGQTKTRLGSPEVKGLVDSLVAASLNTYLDENPAVGRRIIEKALTA
                     SRAREAARKARDLVQRKGVLEGSMLPGKLADCSESNPEYSEIYLVEGESAGGSAKSAR
                     DRRTQAILPLRGKILNVEKARLDKMLAHEEIRVLITALGTGFGDDFDIAKLRYGKVII
                     MTDADVDGAHIRTLLLTFFYRFMPELIDHGHLYIAQPPLYSAQQGRNVTWLYSDAERE
                     AFFSVERKNVNMQRYKGLGEMNPDQLWTTTMDPATRSLLKVEVNDAERADDLFTKLMG
                     DEVPPRKSFIMTHALSATLDV"
     gene            3284..3724
                     /locus_tag="PLX85_02920"
     CDS             3284..3724
                     /locus_tag="PLX85_02920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmO family methyltransferase"
                     /protein_id="HRC62160.1"
                     /translation="MTEDIRLTSIGHVANGEHDVTRQDWSRVRSEVRLREGLGAALLG
                     LRDYSHVIVLGWLDQIPVELRSRLQAYPAGDQRLPLQGSLALRGGGRPNPISFTVCQL
                     RGIEGDTLLVEGLDLVHGTPVLDVKPYIAYYDAVPKAKLPKWAG"
     gene            4015..4269
                     /locus_tag="PLX85_02925"
     CDS             4015..4269
                     /locus_tag="PLX85_02925"
                     /inference="COORDINATES: protein motif:HMM:NF021560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HRC62161.1"
                     /translation="MYIAYDEQGDLLHVEFRSRTDSGYLREVWGDRFVEYDDAGPLGI
                     EFLHASAGVDLDGLPEAEAIAAALRSLATLLGRAGQPARA"
BASE COUNT          655 a         1586 c         1381 g          776 t
ORIGIN      
        1 tccggaacgg cagagtcgtc gcggtcccgc acgccaggcg taccgcgtgg ccgtgcttgc
       61 gcgcgtcgcg cctcgccagg cgcaatgcct ccatcgagaa gtcgactccc gttggcggtg
      121 tgtcaaaccc gacccgctgg tcgaccagcc ggaggagtgc cccgcgcccg cacccaacgt
      181 cgagccagcg ctgctcacgt ctcagcgcga ggttttcggg cagcttcatc agcgggctgt
      241 tcactgccca ctgccccgga gcgctcatca cccagcggat taccgccgaa tgcagatcgc
      301 gtcctccgag ccaccgctcg tactcgcccc cgcgccgcgc ctcgttcacc ccgccatcgt
      361 gtcacgcggt gccagtgcgc acaaaggaag gccagaagac ggtcgactga ctagcgcggg
      421 ccgcgcggcg gcaatatcgg gatcccatcc gaggggaatc gacagtcgtg cgtgtacacg
      481 gtcaggcctc ctgcggaccg tgtcgtcgcc cagaccaacg catcgggggc tccttcagcg
      541 ggcgaaagag ccgactgtgg cagcggagtt ctccggttca gctcattcca gccagaacga
      601 cagccgcacc cccgcgcccc tcgaagccgc ccagtcgcat atcgccgcca actcacgtag
      661 ccgcggcgcg agctcctcgt agcccgcggt cagtcgccgc agctcggcat cgtccgattc
      721 gagggccatc gccaccgcgc gggcagacgc cgcgaggggc gtagcctcct gccaggcgcg
      781 ttcgagctcg cggcggtcgt cctccgactc cgcgtcgcga tccgcgtacg tgagcagcgg
      841 ctttagatcg accttgtgcc ggcgctgcat gtgcatcgct gcctgcgcca gctcatgcat
      901 cccgccgaag cgcatgctga acgcccacga gatgaagaac agcaccaccg tcgggtcgcc
      961 gaggaaccca aacgggcacg gctcgtccgt atcgaactcg acgaacaggc cgcgccctcc
     1021 ggcgggctcg atcgcggagc ggcccaaagg gggcatcgag gcgttagacg tccagcgttg
     1081 ccgacagcgc gtgcgtcatg atgaacgact tccgaggcgg gacctcgtcg cccatgagct
     1141 tcgtgaacag gtcgtccgcg cgttccgcgt cgttcacttc gaccttgagc agcgagcgtg
     1201 tggcgggatc catcgtcgtg gtccacagct ggtcggggtt catctcgccc agacccttgt
     1261 agcgctgcat gttcacgttc ttgcgctcga cagagaagaa cgcctcgcgt tccgcatcgc
     1321 tgtacagcca ggtcacgttg cggccctgct gcgcgctgta gagcggcggc tgcgcgatgt
     1381 acaggtgacc gtggtcgatc agctccggca tgaagcggta gaagaacgtc agcagcagcg
     1441 tacgaatgtg tgcgccgtcc acgtcggcgt ccgtcatgat gatgaccttg ccatagcgca
     1501 gcttcgcgat gtcgaagtcg tcgccgaagc ccgtcccgag cgccgtgatc agcacccgga
     1561 tctcctcgtg cgccagcatc ttgtcgaggc gtgccttctc gacgttcagg atcttgccgc
     1621 gcagcggcag gatcgcctgc gtgcgccggt cgcgtgcact cttcgccgag ccgccggccg
     1681 actcgccctc gacgaggtag atctccgagt actccggatt cgattccgag cagtccgcga
     1741 gcttgcccgg cagcatggag ccctcgagga cgcccttgcg ctgcaccagg tcgcgcgcct
     1801 tgcgcgccgc ctcccgcgca cgcgaggccg tcaacgcctt ctcgatgatg cgccggccca
     1861 ccgccgggtt ctcgtccagg tacgtgttca ggctcgcggc caccagtgag tcgacgagcc
     1921 ccttcacctc gggactgccg aggcgggtct tcgtctgacc ctcgaactgc ggctccggca
     1981 gcttcacgct gatgacggcc gacaacccct cgcgcacgtc gtcgcccgag aggttcgggt
     2041 cgtcgtcctt gagcagcttt gcccgccgcg cgtagtcatt cagggcgcgc gtgagcgccg
     2101 agcgaaagcc cgtcatgtgg ctgccgccgt cgatcgtgtt gatgcaattc gcgaacgagt
     2161 gcaccgactc gctgaacgag ccgttgtact gcagggccac ctcgatcgag atgttgtccc
     2221 gctcgccctg gatcacgatc gggtccgcgt gcagcgggtt acggccgcgg ttcacgtgcc
     2281 gcacgtacgc ctcgatgccg gagtcgaagt agaagttctg ctcctggccg tcgccccgca
     2341 ggtcgatgaa ctggatccac aggcccttgg tcaggtacgc catctcgcgg aaccgcgccg
     2401 cgagggtcgc gaagtcgtag tcgagcgtct cgaagatctc cgagtccggc ttgaacgaga
     2461 tccgtgtccc gttctgctcc ggcttctcgc cgcgcacctt ctcgaggctg cccgtgggcg
     2521 ccccgcgctg gtacgactgg cggtagagcg cgccgtcttt ggcgacctca gcggtcagtt
     2581 cctcggacag tgcgttcacc accgaggcgc ctacgccgtg gaggccaccg gacaccttgt
     2641 acccgccgcc cccgaacttg ccgccggcgt gcagcaccgt cagcaccgtt tcgagggcgg
     2701 agagccccgt cttctcgtgc cggtcgaccg ggatgccgcg cccgttgtcc tggatgctca
     2761 cactgccatc gggctggatg aggacctcga cacggtcgca aaagccggcc attgcctcgt
     2821 cgacggcgtt gtcgacgatc tcgtagatca ggtggtgcag gccgcgctga tcggtcgagc
     2881 cgatgtacat gccgggccgc cggcgcaccg cctcgagacc ctccagcacc tggatattgg
     2941 ccgcggtgta ctcggcgttc tgcgcgttcg tgccatccgc cctgcgtgcg gtcgtcgtca
     3001 tgcgtcctcg ctcaaccgtc tcacacatct ggtcgccggc atcgcaccgg tgcccctgca
     3061 tcgcgacgag ggagagtcgc gaccatggcc gggccgacgc cccgcgacgc ccctgccacg
     3121 ttcctcattc taccacagac gccccctccg ccccgccgcg ctccgaggcg tcagcgcgcc
     3181 cgtcgggaca tcgctcaatc ggctcaacgc gcgctcattc cggtgtatcc gtgcctgcgg
     3241 tgtcgctcgt ggcgaggcct gccggccccc tacaatgcgt ccgatgaccg aggacattcg
     3301 gctcacctcc atcggccacg tcgccaacgg cgaacacgat gtcactcgcc aggactggtc
     3361 gcgtgtccgc agcgaagtcc gcctccgcga gggcctgggt gccgcgctcc tcggtctgcg
     3421 cgactactcg cacgtcatcg tccttggctg gctcgaccag atccccgtgg agctgcgcag
     3481 ccgcctgcag gcctaccccg cgggtgacca gcgactgccg ctccagggct ccctcgccct
     3541 ccgcggcggt ggccgcccca acccgatctc cttcacggtc tgccagctgc gcggcatcga
     3601 gggcgacacg cttctcgtcg aaggcctcga cctcgtccac ggtacccccg tcctcgacgt
     3661 gaagccctat atcgcctact acgacgccgt cccgaaggcg aagctcccga agtgggcagg
     3721 gtgatgcgtc tcctccggcc gccccgtcga ccgccccgtc gaccgatatt taccgccctc
     3781 ctcccggcgg cttccttgaa cgtcatcctg gggctcgtgg ccattcgtca cgacgatctg
     3841 gcgatgggcg tcctcttctt gttgataggg gtccagttcg tgggattcgc ggtctggttc
     3901 gtcgtcctga ggaggcgatt gagcccttac gcaacagggg gtgagtgacc gagacttcgg
     3961 cgactccact catcgtgcga gacgccaggc gctcaggtcc atgaggagca gccgatgtac
     4021 atcgcctacg acgagcaggg cgacctcctt cacgtggagt tccggtcgcg caccgattcc
     4081 gggtatttgc gcgaggtctg gggcgaccgc ttcgtcgagt acgacgacgc cggtccgctc
     4141 ggcatcgagt ttctgcacgc tagtgcaggc gtcgacctcg acgggttgcc cgaagcagaa
     4201 gcgatcgctg ccgccctgcg ttcactggcg acgttgctcg gcagagccgg acagcctgcc
     4261 cgcgcttgac cccctgttgt tccgccctcg gcctgaggtg atgcgatcgc agctacagcg
     4321 cctggatccg ccattgcggg gtccgcagca gcactgaccc catcgggttc tgctccgggc
     4381 tgaacaccag ttgcgacc
//
LOCUS       DAORPG010000086         4396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1444_length_4396_cov_3.153421, whole genome shotgun sequence.
ACCESSION   DAORPG010000086 DAORPG010000000
VERSION     DAORPG010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1444_length_4396_cov_3.153421"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2377
                     /gene="mfd"
                     /locus_tag="PLX85_02930"
     CDS             <1..2377
                     /gene="mfd"
                     /locus_tag="PLX85_02930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRC62162.1"
                     /translation="AASDTLRELRRGDRVVTVTQQAQRYREVLADAEVQAGVVDAVGT
                     PLGSGEFALVQGALPEGWQVATPQGVVSLTTDRELFGFVKRRRTLRRAGSASQRSRFL
                     AEVAPGDFVVHADHGIARFAGIIRRAVGDEERDYLELRYAGDDRLYVPIDQVDRVTRY
                     VGPSEHIPRLTRLGTQEWNHARARVREAVTLVAADLLRLYAARQLLHGHAFREDTPWQ
                     LEMEAAFPFEETEDQLRTIAEVKADMESPRPMDRLICGDVGFGKTEVAVRAAFKAVMD
                     GMQVAVLVPTTVLAQQHERTFRERLAAFPVRVEMLSRFRSDHEAREIIAALKAGEIDI
                     VIGTHRLLQPSVEFANLGLVIIDEEQRFGVAHKERLKRMRLEVDVLSLSATPIPRTLH
                     MSLTGIRDMSSIESAPVDRHPVQTFVAEGDPALVREAILRELDRGGQIYVVHNRVQSI
                     DLLADRLRELVPQARVVVGHGQMSEVLLERVMERFADAEYDILVCTTIIESGIDIPNV
                     NTLIIDNADQLGLAQLYQLRGRVGRSTQQAYAYLLHSRERILSEVAQQRLATIFEATE
                     LGSGFQVALRDLEIRGAGNLLGAEQSGQIASVGFDLYTQMLAEAVEEMKAKTSTAIRE
                     EIPVAQPVAAPVRAARAVKIDLPVSAFIPESYVEEIEARLALYQRIAGLTSLPETEAL
                     RAETADRLGPLPEALEQLFALVRIRLAAGAADVASVRLEEGEVVVTSRDDRPFGPRRL
                     PSLPRAVRVGRTQLRLPKALLGERWLDGIEALLRLVATGTAAAEREAAGVA"
     gene            complement(2463..2717)
                     /locus_tag="PLX85_02935"
     CDS             complement(2463..2717)
                     /locus_tag="PLX85_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62163.1"
                     /translation="MNVQRKIDPDAATPKVFMILVGLALVGFGVSQWRNPATSNERSL
                     WGGGDRFNSVEARRTAGMVTIVFGIVFVVAMAFALITDSN"
     gene            2945..3673
                     /locus_tag="PLX85_02940"
     CDS             2945..3673
                     /locus_tag="PLX85_02940"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRC62164.1"
                     /translation="MNHSRVEAVLFDFDGTLATYRSHLGEYIAAAAEFGVTVTAEALA
                     ATLDDSWRQWQTPEGVDHSTSSGSEAQYNAEVRSRLHIARLAAAGATGDLEGIAARIC
                     ELECAPEAFEVYADTPPALERLQAAGVRVGIVSNHVWRLPEVVEALGLGSAVDFVITS
                     ARVGYRKPHPAIYRIALEAAGCAAEAALFVGDSISHDVEGPRAAGMRAVLIDRRGRYA
                     DGEAIRTLAELEAPGLALPSAERA"
     gene            3670..4143
                     /locus_tag="PLX85_02945"
     CDS             3670..4143
                     /locus_tag="PLX85_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC62165.1"
                     /translation="MKAQAVRVALTPIDERELAEFLAWFDQYRHELEPFDPAGPDPHP
                     LSRYWDALRRDPESQELLWIEADGVRAGFLLARGFEDWPATEDTVVDIAECFVAPEFR
                     RRGVGRAAVEAVLERERARGTVLVEASILPGNADALAFWAALGFAPRAIRTARQP"
     gene            4180..>4396
                     /locus_tag="PLX85_02950"
     CDS             4180..>4396
                     /locus_tag="PLX85_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62166.1"
                     /translation="MIWDVHCHFPRNWDPSVEVEADELLERRADALRSAGVTRASLLC
                     GGRFGLPYEESIAMARRHEDLFVPSAMV"
BASE COUNT          643 a         1359 c         1742 g          652 t
ORIGIN      
        1 ggcagcctcg gatacgctgc gcgagctgcg gcgcggcgac cgcgtggtca cggtcacaca
       61 gcaggcgcag cggtatcggg aggtgctggc ggacgccgag gtgcaggcgg gggtagtcga
      121 cgcggtcggg acgccgctcg gcagcgggga gttcgcgctc gtgcaggggg cgctgcccga
      181 ggggtggcag gtggcgacgc cgcagggggt ggtgtctctg accaccgacc gcgagctgtt
      241 cggcttcgtg aagcggcggc ggacgctgcg gcgcgccggt tcggcctcgc agcgctcgcg
      301 cttcctcgcg gaggtggcgc cgggcgactt cgtggtgcac gccgaccatg gcatcgctcg
      361 gttcgcgggc atcatccggc gcgcggtggg ggacgaggag cgggactacc tcgaactgcg
      421 gtacgcgggc gacgaccggc tgtacgtgcc gatcgaccag gtggaccggg tcacgaggta
      481 cgtggggccc tcggagcaca tcccgcggct gacgcggctg gggacgcagg agtggaacca
      541 tgcgcgagcg cgcgtgcgcg aggcggtcac gctcgtggcg gcggacctgc tgcggctgta
      601 cgccgcgcgg cagctgctgc acggccatgc gtttcgcgag gacacgccct ggcaactcga
      661 gatggaggct gcgttcccct tcgaggagac cgaggaccag ctgcgcacga tcgcggaggt
      721 gaaggccgac atggagtcgc cgcggccgat ggaccgcctc atctgcggcg acgtcggctt
      781 cgggaagacc gaggtggcgg tgcgagccgc gttcaaggcg gtgatggacg ggatgcaggt
      841 ggcggtcctc gtgccgacga ccgtgctggc acagcagcat gagcggacct tccgcgagcg
      901 actggcggcg ttccccgtgc gggtcgagat gctttcgcga ttccgctcgg accacgaggc
      961 gcgggagatc atcgccgcgc tgaaggcagg cgagatcgac atcgtgatcg gtacgcaccg
     1021 gttgctgcag ccgagcgtgg agttcgcgaa cctcgggctg gtgatcatcg acgaggagca
     1081 gcgctttggc gtggcgcaca aggagcggct gaagcgcatg cgcctcgagg tggacgtgtt
     1141 gtcgctctcg gcgacgccga ttcctcggac gctgcacatg tcgctgacgg gcatccgcga
     1201 catgtcgagc atcgagtcgg cgccggtgga ccggcatccg gtgcagacgt tcgtcgcgga
     1261 gggggacccg gcgctcgtgc gcgaggcgat cctgcgcgag ctcgaccggg gcgggcagat
     1321 ctacgtcgtg cacaaccggg tgcagtcgat tgacctgctc gcggatcgcc tgcgggagct
     1381 ggtgccacag gcgcgcgtgg tggtgggcca cgggcagatg tcggaggtgc tcctcgagcg
     1441 ggtgatggag cgtttcgcgg acgccgagta cgacatcctc gtctgcacga cgatcatcga
     1501 gtcgggcatc gatatcccga acgtgaacac gctgatcatc gacaacgccg accagctcgg
     1561 cctcgcccag ctctaccagt tgcgagggcg tgtcgggcgc tcgacgcagc aggcgtatgc
     1621 gtacctgctg cactcccgcg agcgcatcct gagcgaggtg gcacagcagc ggctggcgac
     1681 catcttcgag gcgaccgagc ttggctcggg cttccaggtg gcgctgcggg acctggagat
     1741 ccggggcgcg ggcaacctgc tgggggcgga gcagagcggg cagatcgcct cggtggggtt
     1801 cgacctgtac acgcagatgc tggcggaggc ggtcgaggag atgaaggcaa agacctcgac
     1861 ggccatccgc gaggagatcc ccgtggcgca gccggtggcg gcgccggtgc gggcggcccg
     1921 ggcggtgaag atcgacctgc cggtgtcggc gttcatcccc gagtcgtacg tcgaggagat
     1981 cgaggcgcgg ctggcgctgt accagcggat cgcgggcctc acctcgctgc ccgagacgga
     2041 ggcgctgcgc gccgagacgg cggaccgcct cgggccgttg ccggaggcgc tggagcagct
     2101 gttcgcactc gtgcgaatcc ggctggcggc cggggcggcc gacgtggcct cggtgcggct
     2161 ggaggagggc gaggtggtgg tcacatcgcg cgacgaccgc cccttcggcc ctcggcggct
     2221 gccgtcgctg cctcgcgccg tgcgggtggg gcgaacacag ctgcggctgc caaaggcgct
     2281 ccttggcgag cgctggctgg acgggatcga ggcgttgttg cgcctggtgg cgacggggac
     2341 cgcggcggcg gaacgggagg ccgcgggggt ggcgtagatc gcgcgcctgg cggcgccagg
     2401 gcgtgtacca gatccccctt gatgccacca ctctagagtg acggcggaag cccggcggta
     2461 ggtcagtttg aatccgtgat cagggcgaac gccatcgcga ccacgaagac gataccgaag
     2521 acgatggtga ccatccccgc agttcgtctc gcctcgacgc tgttgaaccg gtctccccca
     2581 ccccacaacg aacgctcgtt ggaggttgca gggttccgcc actgggatac gccgaatccg
     2641 acgagagcta acccgacaag aatcatgaag actttggggg tggcggcgtc aggatcgatc
     2701 ttcctctgta cgttcatggg ccgatcgtct cgccagccgg cgtctattag aacgacactc
     2761 ggcgggtgcc tggtcgctca cgcaacgaaa agtcccgcca cgtgaacggc gacagccgat
     2821 ggaggagcgg gaagctcgaa ttcgccaagt gcgatgccga tgccccaccc aagcataacg
     2881 tccgcccatc cagggaggtt cctccctcgt ggcaacggga cgcgcgagct atcgtttagg
     2941 cacgatgaac cattcgcgtg ttgaggccgt gctgttcgac ttcgacggga cgctggcgac
     3001 gtaccgatcg cacctcgggg agtacatcgc ggcggcggcc gagttcggcg tgacggtgac
     3061 ggcggaggca ctcgcggcga cgctcgacga ctcctggcgg cagtggcaga cgccggaggg
     3121 cgttgaccac tcgacgtcgt ccgggtcgga ggcgcagtac aacgcggagg tgcgctcgcg
     3181 gctgcatatc gcgcggctgg ccgcggcggg ggccacgggc gacctcgaag ggatcgcggc
     3241 tcgcatctgc gagctggagt gcgcaccgga ggcgttcgag gtgtacgcag acacgccgcc
     3301 ggccctcgaa cggctgcagg ccgccggggt acgcgttggc atcgtgtcga atcatgtctg
     3361 gcggctgccg gaggtcgtcg aggcgctcgg cctggggagc gccgtggact tcgtgatcac
     3421 gagcgcgcga gtggggtacc gcaagccgca cccggcgatc taccgcatcg ccctcgaggc
     3481 ggcggggtgc gcggcggagg cggcgctgtt cgtcggcgac agcatctcgc atgacgtcga
     3541 ggggccgcgg gcggcgggga tgcgtgcggt gctgatcgac cgccgggggc ggtatgcgga
     3601 cggtgaggcg atccggacgc tcgcggagct cgaggcgccg gggttggcgt tgccgagcgc
     3661 ggagcgcgca tgaaggcaca ggcggtgcgg gtggcgctga cgccgatcga cgagcgcgag
     3721 ctggccgagt tcctcgcgtg gttcgaccag taccggcacg aactcgagcc gttcgacccg
     3781 gccgggccgg acccgcaccc gctgtcgcgc tactgggacg cgctgcggcg cgacccggag
     3841 tcgcaggagc tgctgtggat cgaggcggac ggggtgcgcg cgggcttcct gctcgcgcgg
     3901 ggcttcgagg actggccggc gacggaggac accgtggtgg acatcgcgga gtgcttcgtg
     3961 gcgccggagt tccggcggcg aggcgtgggc cgcgcggccg tcgaggcggt gctggagcga
     4021 gaacgggcgc gtggcacggt gctcgtggag gcctcgatcc tgccggggaa cgcggacgcg
     4081 ctggcgttct gggcggcgct gggattcgcg cctcgggcga ttcggacggc gaggcagccg
     4141 taaggtagga tcgcgcgagg tgcttcgaag ggaccgcacg tgatctggga cgtccactgc
     4201 catttcccgc gcaactggga tccgtccgtc gaggtcgagg ccgacgaact cctcgagcgg
     4261 cgggctgacg cgctgcggtc ggcgggcgtg acgcgcgcct cgctgctgtg cggcgggcga
     4321 ttcgggctgc cctacgagga gtcgatcgcg atggcgcggc ggcacgagga cctgttcgtg
     4381 ccgagcgcga tggtgg
//
LOCUS       DAORPG010000087         4389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1451_length_4389_cov_3.676050, whole genome shotgun sequence.
ACCESSION   DAORPG010000087 DAORPG010000000
VERSION     DAORPG010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1451_length_4389_cov_3.676050"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="PLX85_02955"
     CDS             complement(<1..813)
                     /locus_tag="PLX85_02955"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRC62167.1"
                     /translation="MTMAGALPEILHSERLDERRFRFGSVDAEGRDVVFGGQLLAQMI
                     TASGALAGGKEVKTIHAVFVRPGTITQPIDIEVTPVNAGRTFASDSVTVWQGDRLCAR
                     GQVLLHAPEPDLIRSELEMPDEPAPEEGTPSRAFVFPGAEMRVVRGTGDSAPGPAELS
                     FWTRYPDAPDDQVASQAILSWATNGLLIGTAMKPHGLNLADAHRTISTGVISNTLTFH
                     DAFNAREWLLLTHDIPYAGRGRVYGRGHVFTREGHIVASFVQDSMVRGIAAGS"
     gene            complement(931..2322)
                     /locus_tag="PLX85_02960"
     CDS             complement(931..2322)
                     /locus_tag="PLX85_02960"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRC62168.1"
                     /translation="MVTIERTVGIDPESIAALKAVIEGEVITAEDASYGSVRQLWNAA
                     FQKFPAVIVRAATTEDVARAVHFANERQAAIAVRSGGHNLAGLSSNDDGVVIDLSQMQ
                     EIDIDPDAQRAWVQPGVTSEVLAPATLPHGLVLTTGDAPTVGIGGLTLGGGIGWFVRK
                     YGLTIDHLLDVEVVTADGRVIVANEQQHADLFWAVRGGGGNFGVVTRYHFRLDPTGLV
                     LAGMIALPMTAEAVRGYVDVATAAPEELSTQLILAPAPPAPFVPADMVGTPIAAVVVC
                     WSGDPEQGRKVLAGFSRLAGGPLFEFVDVMPYNALFEFLREPTRPGLYSSTRSSFMRT
                     VEDGLIEALIAEVPPAMSIAQIRVLGGAMARVPAHATAFAHRDKPYMLMLAGVDLDPQ
                     GAADAAAWVERTWTRAEAWADGVYVNFLADRDGPARVHEAYPSATYQRLADVKAKYDP
                     ENRLRGNQNVRPS"
     gene            2532..2993
                     /locus_tag="PLX85_02965"
     CDS             2532..2993
                     /locus_tag="PLX85_02965"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRC62169.1"
                     /translation="MYVVSDELRGLIESGVAVSIATADAAGHPHLNTAWGPRIGSDGT
                     TATVFIDRARSATTTADLHSTRWIAMTIGDPISYRSIQLKGTQLGVSEASEEDREWVR
                     LHREAFMVSIALIDDPPALGRNMWTEDVIRIDFQIEQAFDQTPGPNAGQPL"
     gene            2990..4339
                     /locus_tag="PLX85_02970"
     CDS             2990..4339
                     /locus_tag="PLX85_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRC62170.1"
                     /translation="MTATQEHVRLRDIPTCFEGIIPSTIATVDSDGTPNVTYLSIVHR
                     IDDDHVALSRQFFKKTDHNTIVNPLAQVGLIEPETGRMFLLDLEYTHTESDGPLFERM
                     RTHLDAVAAQHGMSNVFKLKGVDVCRVLGCTMAPCDFPEPVPPRTPDLETAEAFSRAV
                     AQADDLDELLQATLQACQDLMGFTHAFVMLLDESGERLYTVASAGYSTSGAGSEVALG
                     EGIIGLAAARRQSVRVTNMARELAYSRAVQASSSDEGSGLDSIPLPGLPSVQSQLVTP
                     MLAHRELVGVLVLQSERPGAFQATDEWLMGILASQVALAMEALVGLEPSSNAAASEGE
                     PRPVQVKYYPDDESIFLDNEYLIRGIAGGILWRLLREYQEKRRVEFSNREIRLDQTLA
                     LPDLKDNLEARLILLRKRLDERCGSVVRIEKTGRGRFRLVVSQPLALVTADGSGVDR"
BASE COUNT          729 a         1467 c         1502 g          691 t
ORIGIN      
        1 cgagcccgcg gcgatgcctc ggaccatgct gtcctgcacg aacgaggcga cgatatgacc
       61 ctcgcgggtg aagacgtgac cgcgcccata gacgcggcca cggccggcat acgggatgtc
      121 gtgcgtcagc aggagccact cgcgagcgtt gaaggcgtcg tgaaaggtga gcgtgttgct
      181 gatgacgccg gtcgagatcg tgcgatgtgc gtcagcaagg ttgaggccat ggggcttcat
      241 ggcggtgccg atcaggaggc cgttcgtggc ccaggagagg atcgcctggc tggcgacctg
      301 gtcgtccggg gcgtcggggt agcgagtcca gaacgacagc tccgccgggc ccggggcgct
      361 atcgcctgtg cctcggacca cccgcatctc cgcccccggg aagacgaagg cgcgggacgg
      421 agtcccctct tcgggggctg gctcgtcagg catctcgagc tcgctgcgga tgaggtccgg
      481 ctcaggcgcg tgcaacagga cctgaccgcg cgcgcagaga cggtcgccct gccatacggt
      541 cacggagtcg ctcgcgaacg tgcgaccggc gttgaccggc gttacctcga tgtcgatggg
      601 ctgcgtgatc gtgccgggcc ggacgaacac ggcgtggatc gtcttcactt ccttgccgcc
      661 cgcgagcgcg ccagaagcgg taatcatctg cgcgaggagc tgaccgccga agacgacgtc
      721 gcggccctcg gcatccacgc tgccgaaccg aaagcggcgc tcatccagcc gctccgaatg
      781 cagaatctcg ggtagcgcgc ctgccatcgt catcgccgtc tcccctcggg ccgccgttgt
      841 tcgcgcgcat gatagaccca tgaggagtgc cgatcgtggg acatggagcg ggcccctctc
      901 ggcgcccgct ccatgtggag gacggccgcc ctaggacggg cggacgttct ggttgccccg
      961 gaggcggttt tccgggtcgt acttcgcctt cacatcggca agccgctggt aggtcgcgga
     1021 cgggtacgcc tcgtggacgc gggcggggcc atcgcggtcc gcgaggaagt tcacgtagac
     1081 cccgtccgcc catgcctcgg cgcgcgtcca ggtgcgctcg acccacgcag ccgcgtcggc
     1141 cgcgccctgc ggatcgaggt cgacgcccgc gagcatgagc atgtacggct tgtcgcggtg
     1201 tgcgaaggcc gtcgcgtggg ccgggacccg cgccatggcg ccgccgagga cgcggatctg
     1261 ggcgatcgac atcgcgggcg gcacctctgc gatcagcgcc tcgatgagcc cgtcctcgac
     1321 agtgcgcatg aacgaggaac gcgtcgagct gtacagccct gggcgcgtgg gctcgcgcag
     1381 gaactcgaac agcgcgttgt acggcatcac atcgacgaac tcaaacagcg gaccgcccgc
     1441 gagccgagag aagcccgcca gcaccttgcg accctgctcg gggtcacccg accagcagac
     1501 gacaacggca gcgatcggcg tgccgaccat gtccgcgggc acgaatgggg cgggcggcgc
     1561 gggcgcgagg atgagctgtg tgctcagctc ctcgggagcg gccgtcgcga cgtccacgta
     1621 gccgcgcact gcctcggccg tcatcggcag cgcgatcatc cccgcgagca cgaggccggt
     1681 ggggtcgagc cggaagtggt aacgagtgac gacgccgaag ttgcctcctc cgccgcgcac
     1741 cgcccagaac aggtcggcgt gttgctgctc gtttgcgacg atgacgcggc cgtccgcagt
     1801 gaccacctcg acgtccagca ggtggtcgat ggtcagtccg tacttgcgga caaaccagcc
     1861 gatgccgccg ccgagtgtca ggccgccgat gcccaccgtc ggagcgtcac ccgtcgtcag
     1921 gacaagaccg tgcggcagcg ttgcgggggc gagcacctcg gaggtgacgc cgggctgcac
     1981 ccaggcccgc tgggcgtcgg ggtcgatgtc gatttcctgc atctgcgaga ggtcgatgac
     2041 gacgccgtcg tcgttggaac tcagtccggc gaggttgtga cctccgctgc ggacagcgat
     2101 agcagcctgc cgctcgttgg cgaagtggac ggcacgcgcg acatcctcag tcgtggcagc
     2161 gcggacgatg actgccggga acttctggaa cgccgcgttc cagagctgcc gaaccgaccc
     2221 gtacgaggcg tcctcggcgg tgatgacctc gccttcgatg acggccttga gcgctgcgat
     2281 gctctcggga tcgatgccaa cagttcgttc gatggtgacc atggtgatgc ccctccttcg
     2341 ggtccgcgct gtgcgcgcgt ttgctgtacg accagcatcc cggtgcgggc gatcggcggt
     2401 tcctaaatgg tcgctagccc ttcctaaacg cacgctaaac ggatcggata agattcctaa
     2461 gctctgcctc ggggccgcgg cgcgcctcga aacctccgtt gtccggccgg cgtcttggtg
     2521 gagagagaga gatgtacgtc gtcagcgacg agctgcgggg actgatcgaa tcaggggttg
     2581 cggtgtccat cgcgaccgcg gacgccgccg gtcacccgca cctgaacacc gcatgggggc
     2641 cgcgtatcgg atccgatggc accaccgcga cggtcttcat cgaccgcgcg cggagcgcga
     2701 cgaccaccgc agacctccac agcaccaggt ggatcgcgat gacgatcggt gacccgatct
     2761 cgtaccgctc gatccagctc aaaggtacgc agctcggcgt gtccgaggcg agcgaggaag
     2821 accgcgagtg ggtgcgcctg catcgcgagg cgttcatggt ttcgatcgcg ctgatcgacg
     2881 atccgccggc gctcggccga aacatgtgga ccgaggacgt gatccgcatc gactttcaga
     2941 tcgagcaggc attcgaccag acgccggggc cgaacgcagg gcagccactg tgaccgcgac
     3001 gcaggagcat gtgcggctgc gagacatccc cacctgcttc gagggcatca ttccttcgac
     3061 gatcgccacg gtcgactcgg atggcacccc gaacgtcacg tacctgtcga tcgtgcaccg
     3121 gatcgacgac gaccatgtcg cgctctcacg ccagttcttc aagaagacgg accacaacac
     3181 gatcgtcaac ccgctggcgc aggtcgggct catcgaaccc gagacgggac gcatgttcct
     3241 cctcgacctg gagtacacgc acacggaaag cgacggcccg ctcttcgagc ggatgcgtac
     3301 gcacctcgat gccgtggcgg cgcagcacgg catgtccaac gtcttcaagc tcaagggcgt
     3361 cgacgtctgc cgggtgcttg gctgcacgat ggccccgtgc gacttccccg agcccgtacc
     3421 gccgcgcacg ccggacctgg agacggccga agcattttcg cgggcggtcg cccaggcgga
     3481 cgacctcgac gagttgctgc aggcgacact acaggcctgc caggatttga tggggttcac
     3541 gcacgccttc gtcatgctgc tcgacgagag cggagagcgg ctctacacgg tcgcgagcgc
     3601 cggctacagc acctcaggcg ccggctccga ggtcgcgctg ggcgagggaa ttatcgggct
     3661 ggccgcggcg cgcaggcagt ccgtgcgggt cacgaacatg gctcgcgagc tcgcgtactc
     3721 gcgcgcggtg caggcctcat ccagcgacga gggttcgggg ctcgactcca tcccgctgcc
     3781 gggactcccg tccgtacaga gccagctggt tacaccgatg ctcgcgcacc gcgagctcgt
     3841 cggggtactg gtgctgcaga gtgaacggcc cggcgcgttt caggcgacag acgaatggct
     3901 catgggcatc ctcgcaagcc aggtcgccct cgcgatggaa gctcttgtcg gcctcgagcc
     3961 atcctcgaac gccgccgcat ccgaaggcga accgcggccc gtgcaggtca agtactaccc
     4021 cgacgacgag agcatcttcc tcgacaacga gtacctgatt cgagggattg ccggcggcat
     4081 cctgtggcgg ctgctgcgcg agtaccagga gaagcggcgc gttgagttca gcaaccggga
     4141 gatccggctc gaccagacgc tggcgctgcc cgacctcaag gacaacctcg aggcgcgtct
     4201 catcctgctg cgcaagcgtc tcgatgagcg ctgcggtagc gtcgttcgca tcgagaagac
     4261 cggacgcggc cgcttccgcc tcgtcgtgtc acagccgctc gcgctcgtga cggcggacgg
     4321 ctccggcgtc gatcgctgac cggcggaaca acgagcccgc cttgcggcgg gctctgtcgt
     4381 agctcttcc
//
LOCUS       DAORPG010000088         4377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1458_length_4377_cov_3.379917, whole genome shotgun sequence.
ACCESSION   DAORPG010000088 DAORPG010000000
VERSION     DAORPG010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1458_length_4377_cov_3.379917"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /gene="clpX"
                     /locus_tag="PLX85_02975"
     CDS             <1..975
                     /gene="clpX"
                     /locus_tag="PLX85_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015009728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRC62171.1"
                     /translation="QADIDLEKSNILLIGPTGTGKTAFARTLARILDVPFSIADATAL
                     TEAGYVGEDVENILLHLIQAAEFDIQKAERGIVYIDEIDKIARKTDNPSITRDVSGEG
                     VQQALLKIIEGTVASVPPQGGRKHPHQDFIQINTSNILFIVGGAFEGLDKIIGRRTGE
                     GVRSLGFGVTEHPTAIDRAQLLRHVTHDDLLQFGLIPEFVGRLPVVSTLDPLDVDTMV
                     AIFTQPKNAVAKQFQRLFAIDDVELVFTDDALRAIAEAALSQKTGARGLRTVVEDILL
                     DVMFEMPSRAEIRKCVVSADCVTRNRRPLLLTEGGQALDADEDIPESA"
     gene            1056..1553
                     /locus_tag="PLX85_02980"
     CDS             1056..1553
                     /locus_tag="PLX85_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRC62172.1"
                     /translation="MTDPAAATSPSRVAVRATIHDAEAIHRLVNFWAARGDMLPRTMG
                     EVYENIRDFFVVREDGEAVACGALHITWADIAELKSVAVAEDRQSGGYGSLVVRACIE
                     EGRALGLDRVFALTYRPGFFERLGFQQADVMSLPRKVWNECYRCPKFPGCNEIAMTLE
                     LKPPA"
     gene            1550..2104
                     /locus_tag="PLX85_02985"
     CDS             1550..2104
                     /locus_tag="PLX85_02985"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRC62173.1"
                     /translation="MSDFEVLSAELVHTGHVFEVERASVRLPDGAVVERSLIKHPGAV
                     ALVAIDDQGRWLLVRQYRIGVGETLLEIPAGTRDDGEAPEVTAQRELREETGYAAESL
                     ERLGGTWMVPGYCDEYIHYFLASGLRPDPLDQDADEHLSEPIPMTYDEVLTAVENGEI
                     QDAKTLAAVLLYARRQHRLRPPAD"
     gene            2190..2963
                     /locus_tag="PLX85_02990"
     CDS             2190..2963
                     /locus_tag="PLX85_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HRC62174.1"
                     /translation="MAQASQSMARQRRYGVTQRKDRWWVEPALIGGGFFLFVIYTTVS
                     ALLGDNWKFEIGPYLSPFFEPLIHPGWLPDWLSPAILILWAPLSFRTTCYYYRRAYYR
                     SYFLSPPACAVAEPAKRYSGESKFPLVLQNAHRFALYVALIFVVFLWYGALRSYRFEG
                     EWGIGVGSVILTANAFALMMYTVGCHSFRHLVGGSLDCYSCTSFTKLRHRTWSLVTVA
                     NEHHRLWAWVSLIGVGVTDLYIHLAANGVITDLHTWSSF"
     gene            3001..>4377
                     /locus_tag="PLX85_02995"
     CDS             3001..>4377
                     /locus_tag="PLX85_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HRC62175.1"
                     /translation="MADYETHEYDVLVIGAGGAGLRAAIESSALGARTGLVCKSLLGK
                     AHTVMAEGGLAAALGNVYPQDNWRVHFRDTMRGGKMLNNWRMAQIHAQEAPDRVRELE
                     EWGALFDRTPDGRILQRDFGGHRYARLAHVGDRTGLEMIRTLQQHAVHRGIDVYMECT
                     ITQLLKDGDRISGAFGYWRENGRFVVFRAKAVVLATGGLCKVFAINSNSWEGTGDGHA
                     LATWAGADMIDMECVQFHPTGMVWPLSVRGILVTEGVRGDGGTLRNINGERFMFNYIP
                     PMFAAETADNEEEADRWYNDKVNARRTPDLLPRDEVARAINAEVKAGRGSPHGGVFLD
                     IASRRDASYIQRRLPSMYHQFIQLADVDITRDAMEVGPTAHYVMGGVRVDAESTASTV
                     PGLFAAGEVAGGMHGSNRLGGNSLSDLIVFGRRAGMGAAEYASSLSSLPALNSSEVDA
                     AAAEALAPF"
BASE COUNT          709 a         1582 c         1375 g          711 t
ORIGIN      
        1 caggcggaca tcgacctcga gaagtcgaac atcctcctca tcgggcccac cggcaccggc
       61 aagaccgcct tcgcgaggac gctcgcccgc atcctcgatg tgcccttctc gatcgcggat
      121 gcaaccgcgc tcaccgaggc cggctacgtc ggcgaggacg tcgagaacat cctcctgcac
      181 ctcatccagg cagccgagtt cgacatccag aaggccgagc gcggcatcgt ttacatcgac
      241 gagatcgaca agatcgcgcg caagaccgac aacccctcga ttacccgaga cgtgtctggc
      301 gagggcgtgc agcaggcgct cctcaagatc atcgagggca ccgtcgcgag cgtcccgcct
      361 cagggtggcc gcaagcaccc gcaccaggac ttcatccaga tcaacacctc gaacatcctc
      421 ttcatcgtgg gtggggcatt cgagggcctc gacaagatca tcggccgccg caccggggag
      481 ggcgtacgct cgctcggctt cggcgtgacc gagcacccca cggcgatcga tcgtgcccag
      541 ctgttgcgac acgtcaccca cgacgacctg ctgcagttcg gcctcatccc cgagttcgtc
      601 gggcgcctcc ctgtcgtctc gacactcgac ccgctcgatg tggacacgat ggtcgcgatc
      661 ttcacgcagc ccaagaacgc cgtcgcgaag cagttccagc gtctgttcgc catcgatgac
      721 gtcgaactcg tcttcaccga cgatgccctc cgggcgattg ccgaggcggc cctgagccag
      781 aagaccggag cgcgagggct gcgcacggtc gtcgaggaca tcctgctcga cgtgatgttc
      841 gagatgccgt cacgggccga gatccgcaag tgcgtcgtct ccgccgactg cgtcacgagg
      901 aaccgccgtc cgctgctcct caccgagggt ggccaggctc tcgatgcgga cgaggacatc
      961 ccggagtcgg cctagtcctc cactcacgac cgcaccgccc ccagtgggga tgtccggtcg
     1021 cctatcctta atcccgtgag catcggattg cctcgatgac cgaccccgct gcagccacct
     1081 ccccctcccg cgtcgccgtc cgcgcgacga ttcacgacgc cgaggctatc catcgcctcg
     1141 tcaacttctg ggctgcgcgc ggcgacatgc tcccgcgcac gatgggtgag gtctacgaga
     1201 acatccgcga cttcttcgtg gtccgggagg acggcgaggc cgtcgcctgt ggagcgctcc
     1261 acatcacctg ggccgacatc gcggagctga agtcggtcgc ggtcgccgag gaccgccagt
     1321 ccggcggata cggcagcctg gtcgtacgcg cctgcatcga ggagggtcgt gcgctcggcc
     1381 ttgaccgggt gttcgcgctg acgtaccgac cgggcttctt cgagcgtctc ggcttccagc
     1441 aggccgacgt catgtcgctg ccgcgcaagg tctggaacga gtgctatcgc tgcccgaagt
     1501 tcccgggctg caatgagatc gcgatgaccc tcgagttgaa gccgcccgcg tgagcgattt
     1561 tgaggtcctc tccgccgagc tcgtacatac gggccacgtg ttcgaggtgg agcgcgcctc
     1621 ggtccggctg ccggacggtg ctgtcgtcga acgcagcctg atcaagcacc cgggcgcggt
     1681 cgccctcgtg gcgatcgacg accagggccg ctggctgctc gtacggcagt accgcattgg
     1741 ggtcggcgag acgctcctcg aaatcccggc gggcacccgc gacgacggcg aagcgccgga
     1801 ggtcacggcc cagcgcgagc tgcgcgagga gacaggctac gccgccgagt ccctcgaacg
     1861 ccttggcggc acgtggatgg tgcccggcta ctgcgacgag tacatccact acttcctcgc
     1921 cagcggcctg cgcccggacc ccctcgatca ggatgccgat gagcacctgt ccgaaccgat
     1981 tccgatgacc tacgacgagg tcctgacagc cgtggagaat ggcgagattc aggacgcgaa
     2041 gaccctcgcg gcagtgctcc tgtacgcccg ccgtcagcat cggctacgac ccccggcaga
     2101 ctgacttgac cgtgtacggc gcgaggggaa tagtcacctg agcgcattgc gggccgtggt
     2161 aagcggccac cggtagaaag cacgcgggta tggcacaggc atcccagtcg atggcgcgcc
     2221 agcgacggta cggcgtcacg cagcgcaagg accgctggtg ggtcgagccc gcgttgatcg
     2281 gcggcgggtt cttcctgttc gtcatctaca cgaccgtctc ggcgctcctc ggcgacaact
     2341 ggaagttcga gatcggcccc tacctctcgc ccttcttcga gccgctgatc cacccgggat
     2401 ggctgccgga ttggctgtcg ccggcgatcc tcatcctgtg ggctccgctc tccttccgca
     2461 ccacctgcta ctactaccgg cgggcctact accgctcgta cttcctgagc ccgccggctt
     2521 gcgccgttgc agagcccgcg aagcgctact cgggtgaatc gaagttcccg ctcgtcctgc
     2581 agaacgcgca ccgcttcgct ctctacgtgg cgctcatctt cgtggtcttc ctctggtacg
     2641 gcgcgctgcg ctcgtatcgc ttcgagggcg aatggggcat cggcgtgggt tcggtcatcc
     2701 tgacggcgaa cgccttcgcg ctcatgatgt acacggtcgg ctgccactcc ttccgccacc
     2761 tcgttggcgg cagcctcgac tgctactcct gcacaagctt cacgaagttg cgccaccgca
     2821 catggagcct cgtgaccgtc gccaacgagc atcaccgcct ctgggcgtgg gtcagcctca
     2881 tcggcgtcgg cgtcaccgac ctgtacatcc acctggccgc caacggcgtc atcaccgacc
     2941 tgcacacctg gagcagcttc taacgcgcgt cccgccggga cgtcggatcg agggggaaga
     3001 atggccgact acgagacgca cgagtacgac gtactcgtga tcggcgccgg gggcgccggc
     3061 ctccgcgcgg cgatcgagtc gtccgcgctg ggcgcgcgca ccggcctcgt ctgtaagtcg
     3121 ctgctcggta aggcgcacac cgtgatggcg gagggcggcc tcgccgctgc cctcggcaac
     3181 gtctacccgc aggacaactg gcgcgtccac ttccgcgaca ccatgcgcgg cggcaagatg
     3241 ctcaacaact ggcgcatggc ccagatccac gcccaggaag cccccgaccg cgtgcgcgag
     3301 ctcgaggagt ggggcgccct cttcgaccgc acccccgacg gacgcatcct tcagcgcgac
     3361 ttcggtggcc accgctacgc gcggctcgcc cacgtcggcg accgcaccgg cctcgagatg
     3421 atccgcaccc tccagcagca cgccgtccac cgcggcatcg acgtgtacat ggagtgcacg
     3481 atcacgcagc tgctcaagga cggcgaccgc atcagcggcg ccttcggcta ctggcgcgag
     3541 aacggccggt tcgtcgtctt tcgcgccaag gccgtcgtcc tcgcgacggg tggcctctgc
     3601 aaggtgttcg ccatcaactc gaactcgtgg gagggcaccg gcgacgggca cgccctcgcg
     3661 acctgggccg gcgccgacat gattgacatg gagtgcgtcc agttccaccc cacgggaatg
     3721 gtctggcccc tcagcgtccg gggcatcctc gtgaccgagg gcgtccgggg cgacggcggc
     3781 acgctccgca acatcaacgg cgagcgcttc atgttcaact acatcccgcc gatgttcgcg
     3841 gccgagaccg cggacaacga ggaggaagcg gaccgctggt ataacgacaa ggtcaacgca
     3901 cggcgcaccc ccgacctcct gcctcgtgac gaggtggcgc gagccatcaa cgccgaggtg
     3961 aaggcggggc gcggcagccc ccacggcggc gtcttcctcg acatcgcctc ccgtcgcgac
     4021 gccagctaca tccagcgtcg cctcccgagc atgtaccacc agttcatcca gctcgccgac
     4081 gtcgacatca cccgcgacgc catggaggtc ggccctaccg ctcactatgt gatgggcggc
     4141 gtgcgggtcg acgccgagag cacggcttcg accgtccccg gcctgttcgc cgctggcgag
     4201 gtcgccggcg gcatgcacgg ctcgaaccgc ctgggtggga actcgctctc cgacctcatc
     4261 gtcttcggcc gtcgcgccgg catgggcgcc gccgagtacg ccagctcgct gtcctcgctg
     4321 cctgcgctca actcctcgga agtcgacgcc gcggctgccg aggccctcgc gcccttc
//
LOCUS       DAORPG010000089         4354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1474_length_4354_cov_3.335659, whole genome shotgun sequence.
ACCESSION   DAORPG010000089 DAORPG010000000
VERSION     DAORPG010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1474_length_4354_cov_3.335659"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="PLX85_03000"
     CDS             complement(<1..952)
                     /locus_tag="PLX85_03000"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRC62176.1"
                     /translation="MDNYRFVILGGGMVAGYAARELVERGIGRGELAIVSSDDAMPYE
                     RPPLSKGFLRGDEDEASLFINDRAFYEEHGVALHLETTVEHLDSSGRKLLLRGGGEVG
                     FERLLVATGARVRTLDAPGAGLVGIYYLRGLDDSKAIRAAAQESRRAVVLGGSFIGME
                     VAAVLSQLGLETTLAFPDDRVWKRFFTAEMSAFFERYYEDRGVLLARGERAVRLHGEE
                     RVEAVEFESGRRLPADLVVAGLGVTPAVEVLDGAGLAIDGGVVVNEYLETNVPGVLAA
                     GDVANFLDVRSGTRRRLEHWDNAVEQGKHAARVLLGQREPF"
     gene            1049..2263
                     /gene="hemW"
                     /locus_tag="PLX85_03005"
     CDS             1049..2263
                     /gene="hemW"
                     /locus_tag="PLX85_03005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRC62177.1"
                     /translation="MTTSPRSDLALYLHIPFCTAKCGYCDFNSYAGLEHLVPDYTPAL
                     VRELELWAPAAREFQVRSVFFGGGTPSLSSIEDLEAIVRGIRAHYAVAQDSEWTLEAN
                     PGELTLEHLRGLRAIGLNRLSIGVQSLHDDELQLLDRVHDAARAIEAVEAAREAGFDN
                     LNLDLIFGLIGQPLERWQQTLRRAVALRPEHLSCYALTVEPGTALYYQVAKGQLEPPD
                     PDVVADQYEWTRSFLAEQGYRHYELSNWALPGRECRHNLVYWHAEPYLGVGAGAHSFF
                     AGQRFANVDAPNRYVELVNASYEERQATGGGQLRQIHGGETPDEALLLSDAMILGLRL
                     MEGVSTKAFEARFGVTPEAVFGAAIERHLATGLLERAGDRLRLTSRGQLLANEVFVDL
                     LPEEAAGVSTTG"
     gene            2273..2554
                     /locus_tag="PLX85_03010"
     CDS             2273..2554
                     /locus_tag="PLX85_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62178.1"
                     /translation="MPVDVYRCAHCGREVLVRILAPMTPAEIARRIEEERRPAASGLP
                     GQPAATFRLRDDIWLHVSAERTEVPREVLPEQCPACNRSGTLQPRGTID"
     gene            2968..3600
                     /locus_tag="PLX85_03015"
     CDS             2968..3600
                     /locus_tag="PLX85_03015"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRC62179.1"
                     /translation="MAGAVAFLPLGPTDSAAGSQRGEQLAAATSIEAMRSVFTTSAEA
                     AAESDLNPTPNASPTETAVVPAAFAAVAPPALEGGVGPGLPSATPAELVGQALVASAL
                     QQATPSATAARTAAPTTASSQARSNISLPGIEEALAQSPWPRELWPTVERIIVCESSG
                     NPGAVSPAGYQGLMQVAPWLHGAVPADPVAQLSQAYDVYLKQGWGAWGCY"
     gene            complement(3638..>4354)
                     /locus_tag="PLX85_03020"
     CDS             complement(3638..>4354)
                     /locus_tag="PLX85_03020"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HRC62180.1"
                     /translation="LAVAGGELSSAFVATRPPGHHATHSASMGFCLFNGIAVAAAAAL
                     RSGIERLAIVDWDVHHGNGTQAIFNADPRVLYVSTHASPFYPGTGDIGEIGVGEARGT
                     KVNIPLPHGTGDRGFVDAYERVAVPMLERFEPQLVLVSSGWDSHYRDPLGTLQVSTAA
                     YTRAAAAVFDAARRLCGGKVVVVLEGGYDTHALAWCAGSLVQLLLGEEPTPDPDPTTP
                     PSGPDVAPLIEAVRGAFGLR"
BASE COUNT          694 a         1566 c         1444 g          650 t
ORIGIN      
        1 cgaacggctc gcgctgcccg agcagcactc gcgccgcgtg cttgccctgc tcgaccgcgt
       61 tgtcccagtg ctccaggcgg cgccgcgtgc cggagcggac atcgaggaaa ttggcgacgt
      121 cccccgccgc gaggacgccc gggacgttcg tctcgaggta ctcgttcacc accactccgc
      181 cgtcgatcgc gaggccggcg ccatcgagca cctccacagc cggggtgacg ccgaggcccg
      241 caaccaccag gtccgcaggg agccggcgtc ctgactcgaa ctccaccgcc tcgacccgct
      301 cctcgccgtg cagtcgcact gcgcgttctc cgcgggccag cagcacgccg cgatcctcgt
      361 agtaccgctc gaagaaggct gacatctcgg cggtgaagaa ccgtttccag acgcggtcgt
      421 cggggaaggc aagcgtggtc tcgaggccga gctgggagag gacggcggcg acctccatgc
      481 cgatgaagct cccgccgagg acgacggcgc gccgactctc ctgagccgca gctcggatcg
      541 cctttgagtc gtcgaggcca cgcaggtagt aaatgccaac gagacccgcg ccgggtgcgt
      601 ccagcgtgcg gacgcgagcg ccggtggcga cgagcagccg ttcgaatccc acctcgccac
      661 cgccgcggag caacagcttc cgcccggagc tgtcgagatg ttcgaccgtc gtctcgagat
      721 gcagcgcgac cccgtgctcc tcgtagaacg cgcggtcgtt gatgaagagg ctggcctcat
      781 cctcgtcgcc gcgcaggaag cccttcgaga gcggcggccg ctcgtagggc atcgcgtcgt
      841 cggacgacac gatggccaac tctccacggc cgatgccccg ctcgaccagc tcccgggccg
      901 cgtagcccgc caccatgccg ccaccgagga tcacgaagcg atagttgtcc atggcacacc
      961 tctacccttc gagcgtatgc ccgcccacaa ccgcgcccgg gatgtcgccg tggtggctcg
     1021 cgcccggcgc cgcctgtacc ctgaaggggt gacgacctcc ccccgctcgg acctcgcgct
     1081 ctacctgcat atccccttct gcaccgcgaa atgcggctac tgcgacttca actcgtatgc
     1141 cggcctcgag cacctggtgc ccgattacac gcccgcgctc gtgcgtgaac tcgagttgtg
     1201 ggcaccggcc gcgcgcgagt tccaggtgcg cagcgtgttc ttcggcgggg gcacgccgtc
     1261 gctgtcctcg atcgaggatc tcgaggcgat cgtgcggggc atccgggcgc actacgcggt
     1321 ggctcaggac agcgagtgga cgctcgaggc aaacccgggc gagctgacgc tggagcacct
     1381 gcgcgggtta cgggcgatcg gcctcaaccg gctgtcgatc ggcgtgcagt cgctccacga
     1441 cgatgagctg cagctgctcg atcgcgtcca cgacgccgcg cgggccatcg aggcggtcga
     1501 ggcggcccgc gaagccggat tcgacaacct caacctcgac ctgatcttcg gccttatcgg
     1561 gcagccgctc gagcgctggc agcagacgct gcgtcgcgcg gtcgcgctgc ggcctgagca
     1621 cctgtcttgc tacgcgctga cggtggagcc gggcacggcg ctgtactacc aggtggcgaa
     1681 gggccagctt gaaccgcccg accccgacgt cgtcgccgac cagtacgaat ggacgcgcag
     1741 cttcctcgcg gagcagggct accgccacta cgagctgtcg aactgggcgc tgccgggccg
     1801 cgaatgccgg cacaacctcg tctactggca cgccgagccg tacctgggcg tgggcgccgg
     1861 cgcccactcg ttcttcgcgg gccagcgctt cgccaacgtg gacgcgccca accgctacgt
     1921 ggagctcgtc aacgcgtcgt acgaggagcg gcaggcaacc ggcggcgggc agctgcggca
     1981 gatccacggg ggagagaccc cggacgaagc cctgttgctc tccgacgcca tgattctcgg
     2041 cctccgcctc atggagggcg tctcaacgaa ggccttcgag gcccgatttg gcgtgacgcc
     2101 tgaggccgtc ttcggcgccg ctattgagcg tcacctcgcg accggcctcc tcgagcgggc
     2161 aggtgaccgc ctccgcctga cctcccgcgg ccagctcctc gcgaacgagg tctttgtgga
     2221 tttgctgccc gaagaagccg cgggggtatc cacgaccggc tagactcgaa cgatgccggt
     2281 cgatgtttac cgttgcgcgc actgcggacg agaagtcctg gtccgcatcc tggcgccgat
     2341 gacacccgcc gaaatcgcgc ggcgcatcga ggaggaacgg cgcccggccg cgagcgggct
     2401 gccgggccag cccgccgcga ccttccggct gcgtgacgat atctggctcc acgtctcagc
     2461 agagcgcact gaggtgcctc gcgaggtcct tcccgagcag tgccccgcgt gcaaccgaag
     2521 cggcacgctg cagcctcgcg gcaccatcga ctgaaccgtc cgcgtccacc aaacggcctc
     2581 tgaatgacag gtttgagtag atttctcacg ccatacgtga tattgcgccc gttgtccgta
     2641 taaacaaagc atctgacttc taaaagaccg tacaggagcg tacaggcgca ctttcgcgag
     2701 tacattccag cgccacgtga ggctgcctcc ggttctgccc ggatccctca ggctctccca
     2761 gaacacgtcg gcgagaggcc gaacgaggcc cggcgaattg atcacataac atcgcgatgc
     2821 tcgcgatcgg gccgaatttt cctgcgtttt gggggttcca tcgtcccgcg cggttggcca
     2881 tagtacgccc tcgtggcggg ggtactgaca gcgacactgg cgcgaagcgc gcgccacagc
     2941 cttggtgtta ccgggctggc cctgcttgtg gcgggcgcag tcgccttcct tcccctcggc
     3001 ccgaccgaca gtgccgccgg cagccagcgc ggtgagcagc tcgcggcggc gacttcgatc
     3061 gaggcgatgc gcagcgtctt caccacctca gccgaggcgg ccgccgagtc cgatctcaat
     3121 cccacgccga acgcgagccc gaccgaaacc gcggtcgtgc cggcagcctt cgcggccgtc
     3181 gcgccgcccg cactcgaggg tggcgtggga ccgggactcc cctcggcgac acccgccgaa
     3241 ttggtgggcc aagccctcgt cgcctcggcg ctgcagcagg cgacgccgag cgccaccgcg
     3301 gcccgcaccg ccgctcccac cacggccagc tcgcaggcgc gatcgaacat ctcgctcccc
     3361 ggcatcgagg aggcactggc gcagagcccg tggccacgcg aactgtggcc cacggtggag
     3421 cggatcattg tctgcgagtc gagcgggaac ccgggcgccg tcagtccggc cggctaccag
     3481 gggctgatgc aggtggcgcc gtggctgcac ggcgcggtcc cggcggaccc ggtcgcacag
     3541 ctcagccagg cctacgacgt gtacctcaag caaggctggg gcgcctgggg ctgctactag
     3601 cgccccggtc cgacgagtca gactcgccgg acgttcccta ccgcagtccg aacgctccgc
     3661 gcacggcctc gatcagcggg gcaacatcgg gtcccgacgg cggcgtggtg ggatcggggt
     3721 caggagtcgg ttcctcgccc agcaggagtt gcaccaggct gccggcgcac cacgcgagtg
     3781 catgcgtgtc atagccacct tcgagcacga ccacgacctt gccgccacac agccggcgcg
     3841 ccgcatcgaa gacggccgcc gccgctcgcg tgtaggcggc ggtcgatacc tgaagcgtcc
     3901 ccagcgggtc gcggtaatgc gagtcccacc cgctcgatac cagcacgagc tgcggctcga
     3961 agcgctccag catggggaca gccacgcgct cataggcgtc gacgaaccct cgatcacccg
     4021 tgccgtgcgg cagcgggatg ttcaccttcg tgccgcgagc ctcgcccacg ccgatctcgc
     4081 caatgtctcc cgtcccgggg tagaacggcg aggcgtgcgt gctgacgtac agcacgcgcg
     4141 ggtcggcgtt gaagatggcc tgcgtgccgt ttccgtggtg cacgtcccag tccacgatgg
     4201 cgagccgctc gatgcctgag cgaagcgcgg ccgcggcggc aacggcgatg ccgttgaaga
     4261 ggcagaaacc catcgaggcg ctgtgggtgg cgtgatggcc cggcggacgc gtggcaacaa
     4321 aggcgctgga cagttcaccg cccgcgaccg ccag
//
LOCUS       DAORPG010000090         4342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1486_length_4342_cov_3.522743, whole genome shotgun sequence.
ACCESSION   DAORPG010000090 DAORPG010000000
VERSION     DAORPG010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1486_length_4342_cov_3.522743"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..486)
                     /locus_tag="PLX85_03025"
     CDS             complement(124..486)
                     /locus_tag="PLX85_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HRC62181.1"
                     /translation="MRLGRLSMFLACVAVVAGLAACGGGGDDEAAIAAATVTSGARTT
                     SIHNNKFETPMRVAVGSTVTWVNHDGVGHNVIADDNSFKSGKLDEGDSFSHEFATAGR
                     FSYVCTFHPGMKGVIQVE"
     gene            complement(624..1028)
                     /locus_tag="PLX85_03030"
     CDS             complement(624..1028)
                     /locus_tag="PLX85_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62182.1"
                     /translation="MGRRLVGILLALGGAVGGAFAGRYVARMRQGHAEPEYVVEAKPE
                     PSMGGLRPRDVIPGLIAALRVHDRPWSYLHIPGWLAAFAVNFGLGAMGRDFGPLGGSL
                     PGGGWNWGRPQARWTEARVVSEPAEPTDSFTQ"
     gene            1192..1890
                     /locus_tag="PLX85_03035"
     CDS             1192..1890
                     /locus_tag="PLX85_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006099572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HRC62183.1"
                     /translation="MGTGFERKLVAEFVGTFALIFMGAGSIIVFGGGQADIVAIALAH
                     GLAIALMVSALGHVSGGVFNPAVAIGLWATRRLDTVTTIAYIAAQLVGAAAAAFALKS
                     LFPDALGEAANWGTPLLSSETDFTQGVLIEAILTFFLMTAIFGTALDDRGPKLGGFGI
                     GLIITMDILAGGPLTGAAMNPARTFGPALANGIWDDHLVYWIGPIIGAVVAALLYHYL
                     FLDREAEAAAAKAS"
     gene            1897..2271
                     /locus_tag="PLX85_03040"
     CDS             1897..2271
                     /locus_tag="PLX85_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HRC62184.1"
                     /translation="MYTTIAVATGGALGSVARYGVTRWLASLIGQNLWGTFLVNVTGA
                     FALGLLVGLTEERVSLDPVLRTGIAVGVLGGYTTFSTLMYESMREIEGRALWQSGLNL
                     GGSVAIGLAAVALGLAAGRSAA"
     gene            complement(2439..2882)
                     /gene="dut"
                     /locus_tag="PLX85_03045"
     CDS             complement(2439..2882)
                     /gene="dut"
                     /locus_tag="PLX85_03045"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRC62185.1"
                     /translation="MEGPFELQVKLLRPGARLPARQSDLASGFDLHACLDGGTLEVGG
                     MPVLVPTGVAIAAPPGTDVQIRPRSGLSSRGVMSTFGTIDADYRGELFVTMYCLPSVG
                     SYVIEDGERIAQLVVSRLAEVRWIVVQALDETSRGASGHGSTGRG"
     gene            complement(2953..3471)
                     /locus_tag="PLX85_03050"
     CDS             complement(2953..3471)
                     /locus_tag="PLX85_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRC62186.1"
                     /translation="MSYKIIETCIGCTACVKRCPTSAISGVRDNLHMIDPSLCIDCGA
                     CGVVCPPEAILDDMGDICRTFPRAQAPVAKTIEENCIGSGCELCINICPFDALSLQDS
                     PHNKDFYGMATVDPRKCTGCRLCENSCGWSAIYIDPPREMLKRKIFPVEILTPKKGRG
                     LQRARERQAAEA"
     gene            complement(3631..>4342)
                     /locus_tag="PLX85_03055"
     CDS             complement(3631..>4342)
                     /locus_tag="PLX85_03055"
                     /inference="COORDINATES: protein motif:HMM:NF019095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HRC62187.1"
                     /translation="WMSEGNMLGRNRRNALSAAAALTLALAALAPAIVSGQTAQYSVQ
                     LTGASEVPATTSTATGTFSATLNEAAKTLQWTLNVPTITNATMAHIHAGAAGTNGGVV
                     VTLFSPQSPAGSVSASGTAGPNDLAGPFAGNWDGFVAALKSGGLYVNVHTSANPGGEI
                     RGQVQAPGQGTPTATATASATATSTASSTATASPVAPKTGQAGLVQESSNSGAAIIFV
                     AVAVALVAGSRLLTSRDR"
BASE COUNT          666 a         1532 c         1443 g          701 t
ORIGIN      
        1 ggaatcgagc cgagcaggta cgcaacgatc aggacgagcg cgagcgtcac tccagcctcc
       61 gggcctcgcg tcgagcatac ggttccccgc gagaggccga caagcgcgct ggcagccgcg
      121 ccgttactcg acctggatga cgcccttcat gccgggatgg aacgtgcaga cgtacgaaaa
      181 gcgtcctgcc gtggcgaact catggctgaa gctgtcgccc tcgtcgagct tgcccgactt
      241 gaaggagttg tcgtccgcga tcacgttatg cccgacgccg tcgtggttca cccaggtcac
      301 cgtcgagccg accgcgacgc gcatcggcgt ctcgaacttg ttgttgtgga tcgaggtggt
      361 gcgcgcgccg ctcgtcaccg tcgccgccgc gatcgccgcc tcgtcatcgc cgccaccgcc
      421 gcaggccgcc agcccggcga cgaccgccac gcaggcgagg aacatcgaga gccgtcccag
      481 tcgcatggga accacgcctc ctcggaacac cgcaccgggg aaatgagctg cgcctcgatc
      541 gtagagcgtg gcccgcctac ccgcctcccg aggacaaacg aaatgcgcct cgggcgaggc
      601 gtccgccagg cgtgcaggag tgactactgc gtgaagctgt cggttggctc cgcgggctcc
      661 gacacgacgc gtgcttcggt ccagcgggcc tgcggacggc cccagttcca gccaccgccc
      721 ggcaacgacc ctccgagcgg gccgaagtcg cgccccatcg ccccgagtcc gaagttgacc
      781 gcgaaggcag ccagccaacc ggggatgtgc aggtaggacc acggccggtc gtgcacgcgc
      841 agcgcggcga tcaggcctgg aatgacatcg cggggtcgca acccacccat cgagggctcg
      901 ggtttcgcct cgacgacgta ctccggctcc gcgtgtccct gccgcatgcg ggcgacgtag
      961 cgccccgcga aggcgcctcc gacagcgcca ccgagggcca gcaggatccc gaccagacgt
     1021 ctgcccatcg cgctgctcct tcgatttcac cgtagccgtc tcgtcaacgg gtggtcaacg
     1081 aaacctcccg aattcgccgc agaaaaatga gcaggcgcgt tgacttttca gccgatcttt
     1141 gcttgaatgc tccctaccga gcagcagcta ctgcagcgag gaagtgcggc gatgggtact
     1201 gggttcgagc gcaagctggt tgcggagttc gtcggcacct tcgccctgat cttcatgggc
     1261 gcgggctcca tcatcgtctt cggtggcggt caggccgaca tcgtcgccat cgcgctcgcc
     1321 cacggcctgg ccatcgcgct catggtgtcc gccctcggcc acgtctccgg cggcgtcttc
     1381 aaccccgccg tcgcgatcgg cctctgggcc acgaggcggc tcgacaccgt cacgacgatc
     1441 gcttacatcg cggcgcaact ggtgggggcc gcggcggccg ctttcgccct caagagcctc
     1501 ttcccggacg cgctcgggga agccgccaac tggggcacgc cgctcctgag cagcgagacc
     1561 gacttcaccc agggcgtcct catcgaggcc atcctgacgt tcttcctgat gaccgcgatc
     1621 ttcgggaccg cgctggacga tcgaggtccg aagctgggcg gcttcgggat cggcctcatc
     1681 atcaccatgg acatcctcgc gggcggcccg ctaacgggcg ccgcgatgaa cccggcgcgt
     1741 accttcgggc ctgcgctcgc gaacggcatc tgggacgacc atctggtcta ctggatcggc
     1801 cccatcatcg gcgccgtcgt cgccgccctc ctgtaccact acctcttcct cgaccgcgag
     1861 gccgaggcgg cagcggcgaa ggcgagctga cgggcgatgt acacgaccat tgcggtcgcc
     1921 acgggtgggg ccttgggctc ggtcgcgcgc tacggtgtga cgcgctggct cgccagcctg
     1981 atcggccaga acctctgggg cacgttcctt gtgaacgtca cgggcgcctt cgcgcttgga
     2041 ctcctggtcg ggctgaccga ggagcgcgtg tcgctcgacc cggtcctgcg tacgggcatc
     2101 gccgtaggcg tgctgggtgg ctacacgacc ttctccacgc tgatgtacga gtcgatgcgt
     2161 gagatcgagg gtcgcgcgct gtggcagtcg ggcctcaacc tcggcggcag cgtggcgatc
     2221 ggtctcgcgg ccgtggccct cggcctcgcc gccggacggt ctgcggcatg aaggaggatt
     2281 cgaggtagta cgacgccggc gcctcaaggc gcgcgtcgac cacagacgcc agcatctgcc
     2341 gattaccccc gccgcacggc agatgttggc tagagagcgg gccccagtgg cccgctctcg
     2401 ctttgtccgc cgccgggtgt gggcgccgat acgcggcctc agccgcgccc cgtcgaaccg
     2461 tgtccgctcg cgccccgcga ggtctcgtcg agcgcttgga cgacgatcca gcgcacctcc
     2521 gcgagccggc tgacgacgag ctgggcgatc cgctcgccat cctcgatcac ataactgccg
     2581 accgagggca ggcagtacat cgtgacgaac agctcgccgc ggtagtcggc gtcgatcgtg
     2641 ccgaaggtgc tcatcacgcc ccgcgaggaa agcccggaac gagggcggat ctgcacgtcc
     2701 gtgccgggcg gagcggcgat ggcgacgccg gtcgggacga gcaccggcat gccgcccacc
     2761 tcgagcgtcc cgccgtcgag gcaggcatgc aggtcgaagc cgctggcgag gtcgctctgc
     2821 cgcgcgggga gcctggctcc cgggcggagg agcttcacct gcagctcgaa ggggccttcc
     2881 atgcggcagc cctcgtgaca agaagaaagc ccgccgaggc gggctctctg cgctcgatac
     2941 gaatgaccga ggctacgcct cggcggcctg ccgttcgcga gcacgctgga gtccgcggcc
     3001 cttcttcggc gtcaggatct cgaccgggaa gatcttccgc ttcagcatct cccgaggcgg
     3061 gtcgatgtag atcgcggacc agccgcacga gttctcgcac agccggcagc ccgtgcactt
     3121 gcgcgggtca accgtggcca tgccgtagaa gtccttgttg tgcggcgaat cctgtagcga
     3181 cagcgcgtcg aacgggcaga tgttgatgca cagctcacat ccggagccaa tgcagttctc
     3241 ctcgatcgtc ttcgccacgg gcgcctgcgc gcgcgggaac gtgcggcaga tgtcgcccat
     3301 gtcatcgagg atggcctcgg gcgggcagac gacgccacac gcgccgcagt cgatgcagag
     3361 gctcgggtca atcatgtgca ggttgtcgcg gacgccgctg atcgcgctcg tcgggcaccg
     3421 cttcacgcac gccgtacagc cgatgcacgt ctcgatgatc ttatacgaca acggtttcac
     3481 ccctgtcagg catcggtgcc tattcgtgct cgactctagc accgccccag atcggcatca
     3541 acagacaccg cccgaagacg attcgccaac agtctactgc ggaaatgagt gcggggcgac
     3601 ctgcctgaca gtcgccccgc acggatgccg ctagcggtcg cgactcgtca gcagccggct
     3661 gcccgccacc agtgccaccg ccaccgcgac gaagatgatc gcggccccgg agttcgagga
     3721 ctcttgcacc aggcccgcct gccccgtctt cggggccacc ggcgacgcgg tcgcggtgct
     3781 cgacgcagtc gaggtcgccg tggcgctcgc cgtagctgta gcggtcggcg tcccctggcc
     3841 tggcgcctgc acctgtccgc ggatctcgcc gcccgggttg gcgctcgtgt gcacgttgac
     3901 gtacagcccg cccgacttca gcgccgcgac gaatccgtcc cagttccccg cgaacggccc
     3961 cgcgaggtca ttcggtcccg cggtgccgga agccgagacc gatccggccg gactttgcgg
     4021 ggagaacagc gtgaccacga cgccgccgtt tgtgcccgcg gcgccggcgt ggatgtgcgc
     4081 cattgttgcg ttcgtgatcg tcggcacgtt cagcgtccac tgaagcgtct tggcggcctc
     4141 gttcagcgtt gccgagaagg tgcccgtcgc ggtgctggtg gtggccggga cctcgctggc
     4201 gccggtcagt tgcactgagt actgcgccgt ctgtccgctg acgatggcag gtgcgagcgc
     4261 cgcgagcgcc agggtgagcg cggccgccgc gctcagcgcg ttgcggcgat ttcgtccaag
     4321 catgttgccc tccgacatcc ac
//
LOCUS       DAORPG010000091         4324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1499_length_4324_cov_3.760131, whole genome shotgun sequence.
ACCESSION   DAORPG010000091 DAORPG010000000
VERSION     DAORPG010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1499_length_4324_cov_3.760131"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PLX85_03060"
     CDS             complement(<1..274)
                     /locus_tag="PLX85_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HRC62188.1"
                     /translation="MTAPSRTNDLAYRVALHRVPRMGGVRFALLEATFSNLEDAWHAD
                     AGALHAAGLDVRTAQAITRAHAETDPFAELERLHLAGVDALAPDDPR"
     gene            complement(347..1642)
                     /locus_tag="PLX85_03065"
     CDS             complement(347..1642)
                     /locus_tag="PLX85_03065"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HRC62189.1"
                     /translation="MPLTATIVWLWLRWRWLRIQRHFFGEARTAEATHRFHLSAARSV
                     ARLAVRQQGLIIKTCQFLGSRADILMEEYVEVLSMLQDAVPPRPWADMRPVVEGDLGA
                     PVQQVFAEFDTNAVAAASLAQVYRARTRDGHDVAVKVQYPGIERIVHWDLQTIRFLAN
                     VWSRLETVIDFRPVVEEMERNAPEEVDFVHEGHAAEEIAAMLSTRDDVVVPRIYWQWS
                     GRRVLTMDYLDGTKITDVEALRAAGIDTTQVADSLIDLYSVMVLRRGMFHADPHPGNL
                     LVLPAATPGGPARIGLIDFGLTKRLPEEFRQQVVVLTSAIVAQHRQQVTEAMEGMGFR
                     TRVYDEDTYYALGEAFLGDVLRSGKAYADAALFAEVNVRLGRILRANPLVEVPGDVIL
                     IARVMGLLSGIGRQLDSKTDLIDALIPYLEEETAGEAAS"
     gene            1811..3013
                     /locus_tag="PLX85_03070"
     CDS             1811..3013
                     /locus_tag="PLX85_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRC62190.1"
                     /translation="MAIFNPFLPSFAKNPYPAYASLRASEPVHFSPTLQAWIVTSFAD
                     CERVLREPETFANNPLLATSPMARQFQQQRREFPLGEVPTVLNSDAPVHTRLRGIVSR
                     AFTPRVIEGMRERIEEITSELLDAAPVGEPWDLVEGLALPLPVIVIAELLGVPASDRE
                     LFKRWSTAIADTATNVFSTPESVESTKRASADLIEYLGRVVEERRRSPREDVITALVQ
                     AEDEGSHLTRDELLAFTVVLLIAGHETTINLIGNGTLALLDEPEQARLLHEQPDLIGD
                     AVEEFLRFDSPVQGLVRVATRDVEVGGRAVHRGDALIVMIGAANRDPAHFVEPDRLDV
                     RREDKRHLAFGLGPHFCLGAPLARLEAQVAFRALLTRFASVELAAPPERGGTFVLRGL
                     RTLPVRVN"
     gene            3044..3400
                     /locus_tag="PLX85_03075"
     CDS             3044..3400
                     /locus_tag="PLX85_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011252066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HRC62191.1"
                     /translation="MTDYDPGNIFARILRNEVPSARVYEDDEFVAFKDIAPKAPVHIL
                     VIPRHEGTTAPSALLESDAGWMGRMVVAATKIARAEGLDERGYRLVMNSGPDAHQEVQ
                     HIHLHILGGKSLDSMA"
     gene            3441..3517
                     /locus_tag="PLX85_03080"
     tRNA            3441..3517
                     /locus_tag="PLX85_03080"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3475..3477,aa:Ile,seq:cat)
     gene            3561..4154
                     /locus_tag="PLX85_03085"
     CDS             3561..4154
                     /locus_tag="PLX85_03085"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRC62192.1"
                     /translation="MRDLIRDVVSRHEPILVDPTKDHMPAAVLLLLHERDGVEHLLFQ
                     VRSQHVEFHKGEISLPGGGRDPEDESLLMTALRETEEEIGVHRSHVEVFGRLDDVVTR
                     TNFAMSPYVGAITAETPYPFRIASIEMEMLLEVPVPYLLSGEGWEYMTTPIGEMRNYR
                     HGDHLIFGATARVVSQLVDKLIEAQGGSLSGAQAALR"
     gene            4166..>4324
                     /locus_tag="PLX85_03090"
     CDS             4166..>4324
                     /locus_tag="PLX85_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRC62193.1"
                     /translation="MRHGESEGNVQRIIQGQMDFPLTAAGEVQAEAAGRRLASSGAVA
                     LYTSPLRRA"
BASE COUNT          742 a         1388 c         1474 g          720 t
ORIGIN      
        1 agcgaggatc gtcgggggcg agcgcgtcga ctccggccag atgcagtcgc tctagctcgg
       61 cgaacggatc ggtttcggca tgggcgcgcg tgatggcctg cgcggtgcgt acgtcgaggc
      121 ccgcagcatg gagggcacct gcgtcggcgt gccaggcgtc ctcgagattc gagaaggtcg
      181 cctccagcag cgcgaagcga acgcccccca tgcgcggcac gcgatggagc gcgacgcggt
      241 acgccagatc atttgttcgt gatggagcgg tcacgggctc cagattaaca agcatgggca
      301 agaaatacgc aactgtgatg acgcttcggg ccggagatgg gtgcggctac gaagccgctt
      361 cgcctgccgt ttcttcctcg aggtacggga tgagtgcgtc gatgaggtcc gtcttcgagt
      421 cgagctgccg tccgatgccg gagagcagcc ccatgacccg tgcgatgagg atcacgtcgc
      481 ccggcacctc gacgagcgga ttggccctca ggatgcgtcc gaggcggacg ttgacctcgg
      541 cgaatagcgc tgcgtccgca tacgccttgc cggagcgcag cacatcgccg aggaacgcct
      601 cgccgagtgc gtagtaagtg tcttcgtcat acacgcgcgt acggaacccc atgccttcca
      661 tggcctcggt aacctgctgc cggtgctgcg cgacgatggc gctggtcagg acgaccacct
      721 gctggcggaa ctcctctggc agtcgtttgg tgaggccgaa gtcaatcagg ccgatgcgcg
      781 cgggaccgcc cggagtggcc gccggcagca cgagcaggtt tccggggtgt ggatcggcgt
      841 ggaacatccc acgacgcagc accatcacgc tgtagaggtc gatcagtgaa tcggctacct
      901 gagtggtgtc aatgccggcg gctcgtagcg cttcgacgtc agtgatcttc gtgccgtcga
      961 ggtagtccat cgtcagcacg cgacggccgg accactgcca gtaaatgcgc ggcacgacga
     1021 cgtcatcgcg agtggatagc atcgcggcga tctcctcagc ggcgtggccc tcgtggacga
     1081 agtcgacctc ctcgggggcg ttacgctcca tctcctcgac gacaggccgg aagtcgatga
     1141 cggtctccag ccgcgaccag acgttggcga ggaagcggat cgtctgcagg tcccagtgca
     1201 cgatgcgttc gatgccgggg tactgcacct tcaccgccac gtcgtggccg tcgcgcgtgc
     1261 gcgcgcgata gacctgcgcg agggatgccg cggcgaccgc gttcgtatcg aactcggcga
     1321 acacctgctg tacgggggcg ccgagatcac cctcgacgac aggccgcatg tcggcccacg
     1381 gacggggcgg gaccgcgtcc tgcagcattg agagcacctc gacgtactcc tccatgagga
     1441 tgtccgcgcg gctgccgagg aactgacagg tcttgatgat gaggccctgc tggcgcaccg
     1501 ccaggcgcgc gaccgatctc gcggcactga ggtgaaaacg gtgcgtcgcc tcagccgtgc
     1561 gcgcctcgcc gaagaagtgg cgctggatcc gtaaccagcg ccagcgcaac cacagccaga
     1621 cgatcgttgc ggtcagcggc acgaaccgtc gccatcggcc tgccggtgcg cggacgtaac
     1681 ggtgctcggg tcggacggtc acgaggaagg gggctcccgc cggatgtgcg ttccatccag
     1741 cgtacggggg ggacgatggc ctcgcaacgt gcgcgccgac tcgcgaggca cgcggtacga
     1801 tcgagtggct atggccatct tcaacccctt tctgccgtca ttcgcgaaga acccgtatcc
     1861 ggcgtacgcc tccctgcggg cgtcggagcc cgtccatttc agccccacgc tgcaggcctg
     1921 gatcgtgacc tcgttcgctg actgcgaacg cgtgctgcgc gagccggaga cgttcgccaa
     1981 caacccgttg ctggcgacca gtccgatggc gcggcagttc cagcagcaac ggcgtgagtt
     2041 cccgctcggt gaagtgccga ccgtactcaa ctccgacgcg cccgtgcata cgcggctgcg
     2101 cgggatcgtg agccgcgcgt tcacaccgcg cgtgatcgag gggatgcgcg agaggatcga
     2161 ggagatcacg tccgagcttc tggacgcagc acccgtcggc gagccatggg accttgttga
     2221 ggggctcgcg ctgccgttgc cggtcatcgt catcgcggaa ttgttgggcg tcccggcgtc
     2281 cgaccgtgag ctgttcaaac ggtggtcgac cgcgatcgcc gacaccgcga cgaatgtgtt
     2341 cagcacgccg gagtcggtcg aatccacgaa acgcgcttcg gcggatctca tcgagtacct
     2401 cggtcgcgtc gtggaagagc gccgccgttc acctcgcgag gacgtgatca ccgccctcgt
     2461 tcaggcggaa gacgagggca gtcatctcac ccgggacgag ctgctcgcgt tcaccgtcgt
     2521 gctgctcatc gcgggccacg aaacgacgat caacctgatc ggaaacggca cgctcgcact
     2581 cctcgacgag ccagaacagg cgcggctgct gcacgagcag cccgacctga tcggcgacgc
     2641 ggtcgaggag ttcctccgct tcgacagccc ggtccagggc ctcgtacgcg tcgccacccg
     2701 ggacgtggag gtgggtggcc gtgccgtcca ccgaggcgat gcgctcattg tcatgatcgg
     2761 cgcggcgaat cgagatccgg cgcacttcgt ggagcccgat cgcctcgatg tccggcgcga
     2821 ggacaagcgg cacctggcgt ttggactggg gccgcacttc tgtctcggcg ccccgctcgc
     2881 gcggctcgag gcgcaggttg cattccgggc gctgctgacg cgcttcgcct cggtagagct
     2941 ggccgctccg ccggagcgcg gcggcacgtt cgtactgcgg gggctgcgca cgcttcccgt
     3001 ccgtgtcaac tgacggtcga tcgagtaact gaagagcagg accatgacgg actacgaccc
     3061 cggaaacatc ttcgcgcgca tcctgcgcaa tgaggtgccg agcgcgcgcg tttacgaaga
     3121 cgacgagttt gtggcgttca aggacattgc cccaaaggcc cccgtacaca tcctggtgat
     3181 cccccggcac gaaggcacga cggcgccctc ggcgttgctc gagagcgacg cgggctggat
     3241 ggggcgaatg gtcgtggccg cgacgaagat cgctcgcgcc gagggcctcg acgagcgtgg
     3301 gtaccgcctt gtgatgaaca gcgggccgga cgcgcatcag gaagttcaac acatccatct
     3361 gcacatcctc ggaggcaagt cgctcgattc gatggcatga gccgcacacg gcgtggctat
     3421 actcgacggc gaccagcgga gggcccgtag ctcagcggcc tagagcagtc ggctcataac
     3481 cgatcggtcc caggttcgaa tcctggcggg cctaccacgt ctgagtctgt gtgcgatgcc
     3541 gcagccatcg aggtgatttc atgcgcgatc tgatccgtga tgtcgtaagc cgccacgaac
     3601 ccatcctcgt cgatccgacg aaggatcaca tgccggcggc ggtgctgctg ttgctgcatg
     3661 agcgagacgg cgtcgagcac ctgctgttcc aggtccggag ccaacatgtc gagttccaca
     3721 agggcgagat cagcctgccc ggcggcgggc gcgacccgga agatgagtcg ctgctgatga
     3781 cggcgctgcg agaaaccgaa gaggagatcg gggtgcatcg ctcgcacgtc gaggtcttcg
     3841 gacgcctcga cgacgtggtg acccgcacca acttcgcgat gtcgccgtac gtgggcgcga
     3901 tcacggcgga gacgccgtac ccgttccgca tcgcgagcat cgagatggag atgctgctgg
     3961 aggtgccggt gccgtacctg ttgagtggcg aagggtggga gtacatgacc accccgatcg
     4021 gtgagatgcg caactaccgt cacggcgatc acctcatctt cggcgcaacc gcgcgggtgg
     4081 tctcgcagct ggtcgataag ctcatcgagg cccagggtgg ttcactctcg ggcgcgcagg
     4141 ctgcccttcg ttgacggtca tctacgtgcg gcacggcgaa tccgagggca acgtccaacg
     4201 gatcatccag gggcaaatgg atttccccct cacagctgcc ggtgaggtgc aggccgaggc
     4261 tgcgggacgg cgtctcgcct cgagcggggc ggtcgcgctc tacacgagcc cgctgcgtcg
     4321 cgct
//
LOCUS       DAORPG010000092         4321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1506_length_4321_cov_3.552039, whole genome shotgun sequence.
ACCESSION   DAORPG010000092 DAORPG010000000
VERSION     DAORPG010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1506_length_4321_cov_3.552039"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="PLX85_03095"
     CDS             <1..903
                     /locus_tag="PLX85_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRC62194.1"
                     /translation="VSAAMFHLFTHAFFKALLFLGSGSVIHATERQEVEYLGGLRKKQ
                     PITAATFIIGSLALAGIIPLAGFWSKDEILHTSLNEGSLPVYVVLATTAVLTAIYTTR
                     LVLLTFFGEPRDHHAYDHAHESPRIMTMPLLILSVLAVVAGFVVFTPVGKALGFSGGI
                     TELVFVEEPEKFHFDGAFALGATLSALFGIGIGLAYWWGRAERAERARSWAPELHALL
                     VNRYYMDDLYQAIINRVVLGAAAVIALFDKRVVNESGIDGGAQSVSWFGFRLKFLQTG
                     KIPNYALAMAVGVVALALVAFRTS"
     gene            920..2410
                     /locus_tag="PLX85_03100"
     CDS             920..2410
                     /locus_tag="PLX85_03100"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRC62195.1"
                     /translation="MSSNDGYLLLALFLIPLGTAVLLMFVPSRERGAIIALTGAASFV
                     LFVLSVYLFSRFDYEGAQFQGVKAWTWMENIGFLGEKGIQLKVGLDGIAAPMVLLTGI
                     ITMAGTWVSWKIQHRTKDFFILLYVLVAGVYGTFVMLDLFFFFLLYETAVLPMYLLIA
                     VWGSTRKEYGAMKLMMMLVAASILIFTGIFAIFSEAGLASFDLTRLFGAHYDPTMQKI
                     FFPMIAIGCGTLGGMFPFHAWSPDGHAAAPTAVSMLHAGVLMKLGAFGIIRLGFQILP
                     EGAAFWAPGLITVGITAALYGAISALRQTDLKLMSGFSSVSHMGYVFMGLGTLNVVGM
                     SGAVLQMFAHGVMTALFFLLIGGLYDQSHNRDIPNFSGMAKQMPIWSIFFIIGGMASL
                     GLPGLSGFVAELHIFVGTFRAYPIVGGLAVLTAAITATYLLRMFSQAFFGEMNPRWSG
                     LKEITWTERAGATVLVLSILALGLWPSPWVDRISTTLAHTIPGVSL"
     gene            2407..3900
                     /locus_tag="PLX85_03105"
     CDS             2407..3900
                     /locus_tag="PLX85_03105"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRC62196.1"
                     /translation="MSDLNFDYSLLIPEFILAGTAGVVLLADAFRRELRVSRSVLPGI
                     AAVGALAAGATSLLFVNEHDNFASLVAVDNFTTFFRVLFAATLFVVVLGSYEFVSREI
                     RHQGEFYAMLVLMTLGATFLAAAQELLTAYIGIELLSFSAYVAVSLQKHDPRSGEAGL
                     KYVLLGGVASAILLYGLSLIYGSAGSTSYAEIGARLADGTEDVHLPLLLGLVMLIAGL
                     GFKASAVPFHMWTPDAYEGAPLPVTAMLSTVSKAAAFALFLRFFSGPLLPVVDDWRWM
                     VAVIALLTMVVGNLIAVQQHNIKRLLAYSSIGQVGFMLMAITTVSDASASALLLHMGG
                     YLITNLAVFMAVIAFYNHTGTEEISGFRGLAETNPYLALVITAGLFSLSGMPLLAGFT
                     TKFILFQAAADGGYLWLVSIAVVMSTVSLYYYLMVVRQMYVYEPAGQPDADSATVHDE
                     RRELRWRVSPASYVTTGALLLAIVFVGIYATPLLTAADRAVAVLFPA"
     gene            complement(3978..>4321)
                     /locus_tag="PLX85_03110"
     CDS             complement(3978..>4321)
                     /locus_tag="PLX85_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HRC62197.1"
                     /translation="IEYRAPDAACNPYLVFAGILAAGLAGIEAGDQLPPPLEQDVREL
                     SPADLESRGIVALPASLNEAIRAFERSDLMRQTLGDHIVDSLVKNKRHEWAEYRAQVS
                     QFELDRYFGTL"
BASE COUNT          599 a         1478 c         1381 g          863 t
ORIGIN      
        1 gtctccgccg cgatgttcca cctgttcacc cacgccttct tcaaggcgct gctcttcctc
       61 ggctctggct ccgtcatcca cgcgaccgag cgccaggaag tcgagtacct cggcgggctg
      121 cgcaagaagc agccgatcac cgccgcgacg ttcatcattg gctcgctcgc gctggccggc
      181 atcattccgc tggccggctt ctggtcgaag gacgaaatcc tgcacaccag cctgaacgag
      241 ggcagtctgc ccgtgtacgt cgtgcttgcg acgacggctg tgctgacggc gatctacacc
      301 acgcgcctcg tgctgctcac cttcttcggc gagccgcgtg accaccacgc ctacgaccac
      361 gctcacgagt cgccgcgcat catgacgatg ccgctgctca tcctctcggt gctcgcggtc
      421 gtggccggct tcgtcgtgtt cacgccggtc ggcaaggcgc tgggcttcag cggtggcatc
      481 acggagctgg tcttcgtcga ggagccggag aagttccact tcgatggagc gttcgcgctg
      541 ggcgcgacgc tgtccgcgct gttcggcatc ggcatcggcc tcgcctactg gtggggtcgc
      601 gcggagcgcg ccgagcgcgc ccgcagctgg gctcccgagc tgcacgcgct gctggtcaac
      661 cgctactaca tggatgacct ctaccaggcg atcatcaacc gggtggtgct gggcgccgcc
      721 gcggtcatcg cgctcttcga caagcgcgtc gtgaacgaat ccggtatcga cggcggcgcg
      781 cagagcgtca gctggttcgg cttccgtttg aagttcctgc agaccggcaa gatcccgaac
      841 tacgccctcg cgatggcggt gggtgtggtc gcgctcgcgc tggttgcctt caggacgtcg
      901 taacggcgag gagaggcgat tgtccagtaa cgacggctac ctgctgctag ccctgttcct
      961 catcccgctg gggacggccg tgctgctgat gttcgtgccc tcgcgggagc gtggggcgat
     1021 catcgcgctc accggtgcgg ccagcttcgt cctcttcgtc ctgtccgtgt acctcttctc
     1081 tcgcttcgac tacgaggggg cccagttcca gggcgtcaag gcctggacct ggatggagaa
     1141 catcggcttc ctcggcgaga aaggtatcca gctcaaggtc ggtctcgacg gcatcgccgc
     1201 cccgatggtc ctgctcacgg gcatcatcac catggccggc acgtgggtct cctggaagat
     1261 tcagcaccgc accaaagact tcttcatcct cctctacgtc ctggtcgcgg gcgtctacgg
     1321 caccttcgtg atgctcgacc tgttcttctt cttcctgctg tacgagacgg ccgtcctgcc
     1381 gatgtacctg ctcatcgccg tctggggctc gacgcgcaag gaatacggcg cgatgaagct
     1441 catgatgatg ctcgtcgccg cctcgattct gatcttcacc ggcatcttcg ccatcttcag
     1501 cgaggccggc ctggcgtcgt tcgacctcac tcgcctcttc ggcgcccact acgacccgac
     1561 gatgcagaag atcttcttcc cgatgatcgc catcggctgc ggcaccctgg gcggcatgtt
     1621 cccgttccac gcctggtcgc cggacggcca cgcggcggcg ccaacggcgg tgtcgatgct
     1681 gcacgcgggc gtgctgatga agctcggcgc cttcggtatc atccgcctcg gcttccagat
     1741 ccttcccgag ggggcggcct tctgggcgcc cggcctgatc accgtgggca tcaccgccgc
     1801 gctgtacggc gcgatttcgg cgctgcggca gacggacctc aagctgatgt ccggcttctc
     1861 ttcggtgtcc cacatggggt acgtgttcat gggcctcggc acactgaatg tggtgggcat
     1921 gagcggcgct gtgctgcaga tgttcgctca tggtgtgatg acggccctct tcttcctcct
     1981 catcggtggc ctttacgacc agtcgcacaa ccgcgacatc ccgaacttct cgggcatggc
     2041 caagcagatg ccgatctggt cgatcttctt catcatcgga ggcatggcct cgctgggcct
     2101 gcccgggctc tccgggttcg tcgccgagct gcacatcttc gttggcacct tccgggcgta
     2161 cccgatcgtc ggcggcctgg cggtgctgac ggcggccatc acggcgacct acctgctgcg
     2221 catgttctcg caggcgttct tcggtgagat gaacccgcgc tggtcgggtc tgaaggaaat
     2281 cacgtggacc gaacgggccg gcgcgaccgt gctggtgctc tcgatcctgg cgctcggcct
     2341 ctggccgtcg ccgtgggttg accgcatctc gacgacgctg gctcatacga ttccgggggt
     2401 atcgctgtga gcgacctcaa cttcgattac tcgctgctga tccccgagtt catcctcgcg
     2461 ggaacggccg gggtggtgct gctggccgac gccttccgcc gcgagctgcg cgtaagccgc
     2521 tcggtgctgc cgggcatcgc ggccgtcggc gcactggctg ccggagcgac cagcctcctg
     2581 ttcgtgaacg agcacgacaa cttcgcgagc ctggtcgccg tcgacaactt cacgacgttc
     2641 ttccgcgtgc tgttcgccgc cacgctgttc gtggtggtgc tcggctccta cgagtttgtc
     2701 tcgagggaga tccggcatca gggcgagttc tacgcgatgc tcgtgctgat gacgctgggc
     2761 gcgacgttcc tcgcggcggc gcaggagctg ctcacggcct acatcggcat cgagttgctc
     2821 tcgttctccg cgtatgtggc cgtctccctg cagaagcatg acccgaggtc cggcgaggcc
     2881 ggcctgaagt acgtgctgct cggcggcgtg gcctcggcca tcctgctgta cggactcagc
     2941 ctcatctacg gctccgccgg ctccacgagc tacgccgaga tcggcgcgcg gctcgcggac
     3001 ggcaccgagg acgtccatct gccgctgctg ctcggcctcg tgatgctgat cgcgggcctc
     3061 ggcttcaagg cctcggcggt cccgttccac atgtggacgc ccgacgccta cgagggcgcg
     3121 ccgctccccg tcactgccat gctctcgacc gtctcgaagg cggccgcctt cgcgctcttc
     3181 ctgcgcttct tctccggccc gctgctgccg gtcgtcgacg actggcgctg gatggtcgcg
     3241 gtgatcgccc tgctgacgat ggtggtcggc aacctcatcg ccgtccagca gcacaacatc
     3301 aagcgcctgc tggcctactc ctcgatcggt caggtcgggt tcatgctcat ggccatcacc
     3361 acggtgtcgg atgcctccgc gtccgcgctg ctgctgcaca tgggcggcta cctgatcacg
     3421 aacctcgcgg tgtttatggc cgtgattgcc ttctacaacc acacaggcac cgaggagatc
     3481 agcggcttcc gaggtctggc ggagacgaac ccctacctcg cgctggtcat cacggcgggc
     3541 ctcttctcgc tcagtggcat gccactgctg gccggcttca cgacgaagtt catcctcttc
     3601 caggcggcgg ccgatggcgg ttacctctgg ctggtgagca tcgccgtcgt catgagcacc
     3661 gtgtcgctct actactactt gatggtcgtg cggcagatgt acgtgtacga accggccggg
     3721 cagcccgacg cggactcggc cacggtgcac gacgagcggc gcgagctgcg ctggcgcgtg
     3781 tcgcccgcct cgtacgtcac gaccggtgcg ctgctcctcg ccatcgtgtt cgtcggcatc
     3841 tacgcgacgc cgctgctcac cgcggccgac cgcgcggtag cggtgctctt cccggcctag
     3901 caacgaggac aatcgagaca tcacggaaga gccgccccgg tgggggcggc tcttctgttt
     3961 ctgtgtgcga ggcggggcta cagcgtaccg aagtagcggt cgagctcgaa ctgcgagacc
     4021 tgggcgcggt attcggccca ctcgtggcgc ttgttcttca ccagcgagtc aacgatgtgg
     4081 tcaccgagcg tctgccgcat gaggtccgag cgctcgaacg cgcgaatcgc ctcgttgagc
     4141 gaggcgggca gtgccacgat gccacgcgac tcgaggtcag cggggctgag ctcgcgcaca
     4201 tcctgctcga ggggcggcgg gagctgatcc ccggcctcga tcccggcgag cccggcagcg
     4261 aggatgcccg cgaacacgag gtacgggttg cacgccgcgt ccggcgcccg gtattcgatg
     4321 c
//
LOCUS       DAORPG010000093         4312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1513_length_4312_cov_4.244538, whole genome shotgun sequence.
ACCESSION   DAORPG010000093 DAORPG010000000
VERSION     DAORPG010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1513_length_4312_cov_4.244538"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLX85_03115"
     CDS             <1..217
                     /locus_tag="PLX85_03115"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106157.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRC62198.1"
                     /translation="YRAVTDTEALAAFQALSRLEGIIPALESSHAIAQVLKLQGALRA
                     DDLVLVNLSGRGDKDMGTVAAALGVTL"
     gene            289..1026
                     /locus_tag="PLX85_03120"
     CDS             289..1026
                     /locus_tag="PLX85_03120"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRC62199.1"
                     /translation="MFAEPPELTLPPPRPLRPRIWRARDILGGLGMVVAGFLIVVVGL
                     GVYVGATGDDNTDTPGVVATLFFELLIGATVLFLASRRRLGFRDLGFVLPRRWGFLGL
                     VWAGAYGILVAYQIVLVLLERAGLDVSRLSEGNPLPVEGDSVPLLVVLGIAVVLVAPV
                     SEELFFRGLLFRGMRGYWRLLPSMALSGLVFGAFHGNLSVFLPFAFIGALFAWGFEES
                     DSLLTPILTHMLVNGVSFALSVAGAGN"
     gene            1069..1887
                     /gene="trpA"
                     /locus_tag="PLX85_03125"
     CDS             1069..1887
                     /gene="trpA"
                     /locus_tag="PLX85_03125"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRC62200.1"
                     /translation="MTDRLAEMFARTRAEGRPAVIPFVPGGWPEPDATLEIVRAAVEG
                     GADAMEIGVPFSDPIGDGVTNQIAYQQALEQGVTPPAILDAVRAIRAAGIRIPLLLMG
                     YCNPLFAYGLDRFVHDAAEAGVDGFIVVDMPPDEAGEFEPLVRAAGLHMVYLLAPTST
                     AERIALVAEHASGFIYCVSVTGVTGTRSQLSDELPAFLARVRQQTDVPLAVGFGISTR
                     EHVESVGQIADAAVIGSAFVQAVTSAPRAERPRVVRAYVEGITGRGNGHTPGNG"
     gene            2003..2389
                     /gene="aroH"
                     /locus_tag="PLX85_03130"
     CDS             2003..2389
                     /gene="aroH"
                     /locus_tag="PLX85_03130"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRC62201.1"
                     /translation="MLVRGFRGATTADANTREAILDATAELLDALIRANDIQHDHVAS
                     IIFTTTPDLNAEFPAVAARAAGWTDVALMCGHEMGVPGSLARCVRILMHVNTELGLDQ
                     VKHVYLRGAKALRPDLAASEPTEVTA"
     gene            2386..3426
                     /gene="aroF"
                     /locus_tag="PLX85_03135"
     CDS             2386..3426
                     /gene="aroF"
                     /locus_tag="PLX85_03135"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRC62202.1"
                     /translation="MIVVMSTTRTPEQQAAVITRIQDLGLRAQEISGVERSVIAVLGQ
                     VYPELKDELSTLDGVEEVLRVSKPYKLSSREVRPNDTVVELSHGVRIGGGRPVVMAGP
                     CSIETEEQLWAAARGVKEAGAHILRGGAFKPRSSPYAFQGLGVPGLKLLRQCGDEFGM
                     PVITELLSVRDVAAVAEYSDILQIGARNMQNFVLLEEAGKTGKPVLLKRALSGTIEEW
                     LLASEYILNQGNPNVILCERGIRTFETATRNTLDLNAIALVKRLSHLPVISDPSHGTG
                     KWYLVRPMAMASIAVGADGLIIEVHPNPDQALSDGAQSLTPANFAELMREGSALAASI
                     GRPFADVPVAVA"
     gene            3440..4267
                     /locus_tag="PLX85_03140"
     CDS             3440..4267
                     /locus_tag="PLX85_03140"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HRC62203.1"
                     /translation="MPDEQDAIEAAAAQPSEAAPGGFVLQLPVFEGPLDLLLTLLEDR
                     QLDITEVSLLAVTEQYLAHLREAERLNLDAFADFIGIGARLLLLKSRALLPRDPEAEP
                     SAAEDEADPEALLAALQEYRRFREAAEHLRELEAEGRTVFRRSAAPPEIPPTTGLDGT
                     TLNSLMDVLREVLERLPPEKPIPRGLPRESVRLADRVRALVDLLDRDGSTSFRSLITS
                     ARTRLAVIVEFLAVLELVKARYLEARQSEAFGDIDLVKVLGGAPPSAEEIAQDFTGL"
BASE COUNT          615 a         1639 c         1364 g          694 t
ORIGIN      
        1 gtaccgcgcc gtgacggaca ccgaggcgct cgccgccttc caggcgctct cgcgactcga
       61 gggcatcatc cccgccctcg agtcgtcaca cgccatcgcc caggtgctga agctgcaggg
      121 agcgctgcgc gccgacgacc tcgtactggt gaacctcagc ggccgcggcg acaaggacat
      181 gggcaccgtc gccgccgccc tcggcgtgac cctctagggc ggactcctcc accgggcctc
      241 tcgcgaggcg acacgcgccg ccgtccttgc tcgtgatacg ctcgatgcgt gttcgcggag
      301 ccgcctgaac tcacactgcc tccgccccgc cccctccgac cgcgcatctg gcgagcccgc
      361 gacattctcg gcgggctggg catggtcgtc gccggcttcc tcatcgtcgt ggtcggcctt
      421 ggcgtctatg tgggggccac gggagacgac aacaccgaca cgcccggcgt cgtcgccacc
      481 ctcttcttcg agctgctcat tggcgccacc gtcctgttcc tcgcctccag gcgacgcctc
      541 gggttccgcg acctcggctt cgtgctgccg cgccgctggg gcttccttgg cctcgtctgg
      601 gccggcgcct acggcatcct cgtggcctat cagatcgtcc tcgtcctgct cgaacgcgcc
      661 ggactcgacg tctcgaggct gagcgagggc aacccgttgc cggtcgaagg cgacagcgtc
      721 ccgctgctcg tcgtgctggg catcgccgtc gtcctcgtcg cgccggtcag tgaggagctc
      781 ttcttccgcg ggctactctt ccgaggaatg cgtggctact ggcggctgct cccctcgatg
      841 gcgctcagcg gcctcgtctt cggcgccttc cacggcaacc tcagcgtctt cctgccgttc
      901 gccttcatcg gcgccctctt cgcctggggc ttcgaggagt ctgactcgct gctcacgccc
      961 atcctcacgc acatgctcgt gaacggcgtc tcgttcgcgc tcagcgtcgc gggggcaggc
     1021 aactaggctg cctcgcatcc caatccgcgc gctgctaccc tcgcctgcat gaccgaccgc
     1081 ctcgccgaga tgttcgcgcg tacccgcgcc gagggccgcc ccgccgtcat ccccttcgtc
     1141 cccggtggct ggcccgagcc ggacgccacc cttgagatcg tgcgcgccgc ggtcgagggc
     1201 ggggcggacg cgatggagat cggcgtcccc ttcagcgacc ccatcggcga cggtgtcacg
     1261 aaccagatcg cctaccagca ggccctcgag cagggggtga cgccccccgc gatcctcgat
     1321 gccgtgcggg ctattcgcgc cgccggcatt cgcatcccgt tacttcttat gggatactgc
     1381 aacccgctgt tcgcctacgg gctggacaga ttcgtgcacg acgcggccga ggcgggcgtc
     1441 gatggcttca tcgtcgtcga catgcccccg gacgaggcgg gcgaattcga gcccctcgtc
     1501 cgcgccgcgg gcttgcacat ggtgtacctg ctcgccccca cctcgaccgc cgagcgcatc
     1561 gcgctcgtcg ccgagcacgc aagcgggttc atctactgcg tgagcgtcac gggcgtgacg
     1621 ggcacgcgca gtcagctctc cgacgagctg ccggcgttcc tcgcgagggt ccgccagcag
     1681 accgacgtac cgctggcggt cggcttcggc atctcgacgc gcgaacacgt cgagtccgtg
     1741 ggccagatcg ctgacgcggc cgtgatcggg agcgctttcg tgcaggcggt gacttcggcg
     1801 ccgcgagcag aacgcccgag ggtcgtgcgg gcgtacgtgg aggggattac cggtcgtggg
     1861 aatggccaca ctccgggcaa cggctgaccg tcgcgtcaca cgcgacgtct gcgcgcgccc
     1921 gtggccgacc gcggccgcgt cgtttggggc gggctcacca ggacgccagc tccacgcaca
     1981 ctgaacatcg gagccccgcc ccatgctcgt ccgaggtttt cgaggcgcta ccaccgccga
     2041 tgccaacacc cgcgaggcca tcctcgacgc aaccgctgag ttgctcgatg cgctgatccg
     2101 cgcgaatgac atccagcacg accacgtcgc ctcgatcatc ttcaccacca cgcccgacct
     2161 gaacgccgag ttcccggccg tcgccgcgcg cgccgccggc tggaccgacg tcgcgctcat
     2221 gtgcggccac gagatgggcg ttccgggcag tctcgcccgt tgcgtgcgca tcctgatgca
     2281 cgtcaacacc gagctggggc tcgaccaggt caaacacgtc tacctgcgag gtgcgaaggc
     2341 gctccgcccc gacctcgccg ccagcgaacc cacggaggta acagcatgat cgtcgtgatg
     2401 agcaccaccc gcacgcccga gcagcaggcg gccgtgatca cccgtatcca ggacctcggc
     2461 ctgcgtgccc aggaaatctc cggcgtcgag cgcagcgtca tcgccgtcct cggccaggtc
     2521 tacccggagc tcaaagacga gctctccacc ctcgacggcg tcgaggaagt cctgcgcgtc
     2581 tcgaagccgt acaagctctc gagccgcgag gtgcgcccca acgacaccgt cgtcgagctg
     2641 tcgcacggcg tccgcattgg cggcggccgt ccggtcgtca tggcgggccc ctgctcgatt
     2701 gagaccgagg agcaactctg ggccgcagcc cgaggcgtga aggaggccgg ggcgcacatc
     2761 ctgcgcgggg gcgccttcaa gccgcgctcc tcgccctacg ccttccaggg ccttggtgtg
     2821 cccggcctga agctgctccg ccagtgcggc gatgagttcg ggatgccggt catcacggag
     2881 ctgctctccg tccgcgatgt cgcggccgtc gccgagtact cggacatcct ccagatcggc
     2941 gcgcgcaaca tgcagaactt cgtcctcctc gaggaggccg ggaagacggg caagcccgtc
     3001 ctgctgaagc gcgctctctc cgggacgatc gaggagtggc tcctcgccag cgagtacatc
     3061 ctcaaccagg gcaacccgaa cgtgatcctc tgcgagcgcg gcatccgcac cttcgagacc
     3121 gcaacccgca atacgctcga cctgaacgcg atcgcgctcg tgaagcggct gagccacctc
     3181 cccgtcatct ccgacccgtc ccacggcacg ggcaagtggt acctcgtccg ccccatggcg
     3241 atggcgagca tcgccgtcgg cgccgatggc ctcatcatcg aggtgcaccc caaccccgac
     3301 caggcgctct cggacggtgc ccagtcgctc accccggcca acttcgccga gctgatgcgg
     3361 gaggggtcgg cgctcgccgc ctccatcggc cgccccttcg ccgacgtacc ggtggccgtc
     3421 gcctagcggc gggccatccg tgcccgacga gcaggacgcc atcgaagccg ccgcggcgca
     3481 gccctccgag gctgcacccg gcggcttcgt gttgcagctc cccgtcttcg aggggccgct
     3541 cgacctgttg ctgaccctgc tcgaggaccg ccaactcgac atcaccgagg tctcgctcct
     3601 cgccgtcaca gaacagtacc tggcgcacct ccgcgaggcg gagcgactca acctcgatgc
     3661 cttcgctgac ttcatcggca tcggcgcccg cctgctgctg ctgaagtcgc gcgcgctgct
     3721 cccgcgcgac cccgaggccg aaccctccgc cgccgaggac gaggcagacc ccgaggccct
     3781 gctcgccgcg ctccaggagt accggcgctt ccgcgaagcg gccgagcatc tgcgcgaatt
     3841 ggaagccgag ggccgcaccg tcttccgccg cagcgcagcg ccgccggaga tcccaccgac
     3901 gaccggcctc gatggcacga cgctgaactc gttgatggac gtgctgcggg aggtattgga
     3961 gcggctgcca ccggagaaac caatccctcg aggcttgccg cgcgagtccg tccgtctcgc
     4021 cgaccgcgtg cgggccttgg tcgacctgct cgaccgggac gggagtactt ccttccgctc
     4081 gctcatcacc tcggcccgca cccgcctcgc cgtgatcgtc gagttcctgg ccgtgctcga
     4141 actcgtcaag gcgcgctacc tcgaggcgcg tcagtccgag gcgtttggcg acatcgatct
     4201 cgtgaaggtc ctcggcggcg cgccaccctc cgccgaggag atcgcccaag actttaccgg
     4261 cctgtaggcc cgccgccacg tttgaacgcg cgattcgcgc ggtgctacga tc
//
LOCUS       DAORPG010000094         4274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1547_length_4274_cov_3.517895, whole genome shotgun sequence.
ACCESSION   DAORPG010000094 DAORPG010000000
VERSION     DAORPG010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1547_length_4274_cov_3.517895"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PLX85_03145"
     CDS             complement(<1..603)
                     /locus_tag="PLX85_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRC62204.1"
                     /translation="MTTNIQAREAVGEYKWGFHDEEQPLFIAEKGLNEDVIRGMSKMK
                     GEPEWMLEQRLEALATFRQLHDPTWAGSPELLQAIDFDNIHYYVRATDRDATKWEDVP
                     EYIKDTFDKLGIPEAERQFLAGAGAQYDSEVVYHNIREDLKKQGVVFLGMDQGLAEHE
                     DLVREYFGTVVPAGDNRYAALNSAVWSGGSFIYVPPGVHVD"
     gene            complement(600..1391)
                     /gene="sufC"
                     /locus_tag="PLX85_03150"
     CDS             complement(600..1391)
                     /gene="sufC"
                     /locus_tag="PLX85_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014123956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRC62205.1"
                     /translation="MSEPILEVRGLQVSIADKPEIHIVKGVDLTVGRGEVHALMGPNG
                     SGKSTLASTIAGSPSYSVDAGQILFKGEDVTEWSADKRARNGLFLSFQYPTPIPGVTM
                     VNLLRAALQARRGQEVPAREFLVELRSTLGQLKMDESFARRYVNDGFSGGEKKRAEIL
                     QLGMLKPDLAILDETDSGLDVDALRTVAEGVNALRTAEMGILLITHYERILNYIQPDF
                     VHVLVNGRIVRSGDASLAKQIETTGYDPILQELGLSREKEEAAAR"
     gene            complement(1416..2114)
                     /locus_tag="PLX85_03155"
     CDS             complement(1416..2114)
                     /locus_tag="PLX85_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HRC62206.1"
                     /translation="MERTRDEVVRILHDRGPSSVAEVAEAIGVSSGSVRRHMDIMIAE
                     GLVEMRLERQPRGRPVTRYFLSEAGEERSAAAHYARLLDRLYPALTRLPADAVSGHNG
                     VWVLEQVFNGVAEDIARAYAPRISASNLDDRVAQVADVLRAEGILNDVVDEGERIRLR
                     NIGCPYRSTAEGTHVACAADRRTIELLLGESVEQVATIVEGSPTCEYLVVRNHQEQDE
                     DVDVLEAHPELVRN"
     gene            2345..2515
                     /locus_tag="PLX85_03160"
     CDS             2345..2515
                     /locus_tag="PLX85_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62207.1"
                     /translation="MSHHGRPGHESAPKNRRESDRDEEQQGETVEDKGGSGAPQGGQI
                     DGRQGDQQGLKR"
     gene            complement(2528..2686)
                     /locus_tag="PLX85_03165"
     CDS             complement(2528..2686)
                     /locus_tag="PLX85_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62208.1"
                     /translation="MAKQGQHQPSKTSKTEQTREKKAAEGGSTRSRRPRSGRSGSDSN
                     ASSGTRGH"
     gene            complement(2769..3869)
                     /locus_tag="PLX85_03170"
     CDS             complement(2769..3869)
                     /locus_tag="PLX85_03170"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRC62209.1"
                     /translation="MSADGIARVESLFEQQLAVGWHPGAQLAVFRDGVLALELTGGLA
                     APGRRLTRDDPMLLFSATKPIAAACVHLLHERGRLSYDTPVASFWPQFAHGGKRAATV
                     RHVLTHQGGFPQLAYDFDWGRVDDWAYVTAQTASISAAWKPGAAVGYHPITYGWALGE
                     VVRRIDGRLPREFMRDEVFAPLGIADSISLGLSGARVADRVPVYAMSEETRHDPTGSL
                     GATSRIVEVFNTEAMARAQAPAVNAYGTARALARFYAALLPSAPGRPLLRLATIEEST
                     REQVETARDRTQDVPKRYGLGFYVSGLRGDPFDYHEGPGVFGHAGQQSSVGYADPRYG
                     LAVAYITNGLQAPGIVTRRMAEMAAALREACG"
     gene            complement(3902..>4274)
                     /locus_tag="PLX85_03175"
     CDS             complement(3902..>4274)
                     /locus_tag="PLX85_03175"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRC62210.1"
                     /translation="NAIGTAEALAHFYAMLERGGALGGRRILEAGTVAEATRVHAEAV
                     ADRTSGLPSSYGLGFMAGGIWEPYCTPGVFGHGGQQCAVAYADPSRGLALAYVTNGLQ
                     DPANYMRRIEDVVAAARAACE"
BASE COUNT          667 a         1470 c         1433 g          704 t
ORIGIN      
        1 gtcgacgtgc acgcccggcg gcacgtagat gaacgagcca cccgaccaca cggccgagtt
       61 gagcgccgcg tagcggttat cgcccgccgg aacgaccgtc ccgaagtact cccgcacgag
      121 gtcctcgtgc tcggcgaggc cctggtccat gcccaggaag accacgccct gcttcttcag
      181 gtcctcgcgg atgttgtggt agaccacctc ggagtcgtac tgggcgccgg cgcccgcgag
      241 gaactggcgc tccgcctcgg ggatgccgag cttgtcgaag gtgtccttga tgtactccgg
      301 gacatcctcc cacttcgtgg cgtcacggtc ggtggcgcgc acgtagtagt ggatgttgtc
      361 gaagtcgatg gcctgcagca gctcagggct gccggcccag gtcgggtcgt gcagctggcg
      421 gaaggtcgcg agcgcctcga ggcgctgctc gagcatccac tcgggctcac ccttcatctt
      481 cgacatacca cggatgacgt cttcgttgag gcccttctcg gcgatgaaga gcggctgctc
      541 ctcgtcgtgg aagccccact tgtattcgcc gaccgcttcg cgggcctgga tgttcgtcgt
      601 catcgggcgg ccgcctcttc cttctcacgg ctgagaccga gctcctgcag gatcgggtcg
      661 tagccggtcg tctcgatctg cttcgcgagg ctcgcgtcgc cggagcggac gatgcgcccg
      721 ttcacgagca cgtgcacgaa gtcaggctgg atgtagttca ggatccgctc gtagtgggtg
      781 atgagcagaa tgcccatctc cgccgtacgc agggcgttca cgccctcggc gactgtgcgg
      841 agcgcgtcca cgtcgaggcc ggagtcggtc tcatcgagga tggcgaggtc cggcttcagc
      901 atgccgagct ggaggatctc ggcgcgcttc ttctcgccgc cggagaaccc gtcgttcacg
      961 tagcggcgag cgaaggactc gtccatcttc agctgcccga gggtggaccg cagctcgaca
     1021 aggaactcgc gggccgggac ctcctggcca cgccgcgcct ggagggccgc gcgcagcagg
     1081 ttcaccatcg tcacgccggg gatcggcgtc gggtactgga aggagaggaa gaggccgttg
     1141 cgggctcgct tatcggcgct ccattcggtg acgtcctcgc ccttgaagag aatctggccg
     1201 gcgtcgacgg agtacgacgg gctgcctgcg atcgtgctcg cgagcgtgct cttgccggag
     1261 ccatttggcc ccatgagcgc gtgcacttcg ccacggccga ccgtgaggtc gacgcccttg
     1321 acgatatgga tctccggctt gtccgcgatg ctgacctgca ggccgcgcac ctcgaggatg
     1381 ggttcactca tcggctcggt tgcccttccg ttcgactagt tccgcaccag ttcggggtgc
     1441 gcctcgagga cgtccacgtc ctcgtcctgt tcctggtggt tgcgaacaac gaggtactcg
     1501 caggtcggcg agccctcgac gatcgttgcg acctgttcca ccgactcgcc cagcagcagc
     1561 tcgatcgtcc gccggtccgc cgcgcatgcg acatgcgtgc cctccgcggt cgagcggtac
     1621 ggacatccga tgttgcgcag ccggatgcgc tccccctcgt cgacgacatc gttgaggatg
     1681 ccttcggcac ggaggacgtc cgccacctgc gccacgcggt catcgaggtt gctcgcgctg
     1741 atccgaggcg cgtacgcgcg ggcgatatcc tccgcgacac cgttgaacac ctgctccagc
     1801 acccagacgc cgttgtggcc cgacaccgcg tcggcgggga gcctcgtcag cgcgggatag
     1861 agccggtcca ggagtcgcgc gtagtgcgcg gcagccgaac gctcctcgcc cgcctcgctc
     1921 aggaaatacc tcgtgacggg gcgccctcgc ggctgccgct cgaggcgcat ctcgacgagg
     1981 ccctcggcga tcatgatgtc catgtgccgc cgcaccgagc ccgatgacac gccaatcgcc
     2041 tcggcgactt ccgccaccga ggaggggcca cggtcatgca ggatccgtac gacttcgtcg
     2101 cgagttcgtt ccaccgcagt gctcgcagtc tgccgggggg atcgccaggc cctcggcggt
     2161 gtcgctggct tcgcaggtcg agtccagtgt ctgcccgcgc cgagcctatg tcaatgattc
     2221 tttcaattaa ttgcgaaaaa gtcccggaaa gagcccccgg gcgcgatcgg gggcttgccg
     2281 gcgcgcgctt gctgcgcgag gcagaatggt tcgaacgcag gaaacatgat gaggagagtt
     2341 agttatgtcg catcacggga gacccggaca cgaatccgcg ccgaagaacc gccgcgaatc
     2401 cgaccgcgac gaggaacaac agggcgaaac ggtcgaggac aagggcggct caggtgcgcc
     2461 ccagggcggt cagatcgacg gccgccaggg cgaccagcag gggctcaagc gctagaccga
     2521 ccggccgtca gtgaccacgc gtcccgctgc tcgcgtttga gtcgctgcca ctgcggccgg
     2581 aacgaggtcg gcggctgcgc gtgctgccac cttcggcagc cttcttttcg cgtgtctgct
     2641 cggtcttgct cgtcttgctc ggttgatgct gaccctgctt cgccatgacg cacctcctgg
     2701 tgactatcgt cctagtctag tgaacccgtg atccatggca gggggcgaag ggcctcggat
     2761 cgaggtgact agccgcaggc ctcgcgcagg gcggccgcca tctcggccat gcgccgggtc
     2821 acgatgccgg gcgcctgcaa cccgttggtg atgtacgcca ccgccaaccc gtagcggggg
     2881 tcggcgtagc cgacggatga ctgctgcccg gcatgtccga acacccccgg cccctcgtgg
     2941 tagtcgaacg ggtcgccccg cagaccgctg acgtagaacc cgaggccata gcgcttcggg
     3001 acgtcctgcg tccggtcgcg agccgtctca acctgctccc tcgtggactc ctcgatggtc
     3061 gcaaggcgta gcagcggtcg accgggagca ctgggcagca gtgcggcgta gaagcgcgcg
     3121 agcgcgcgcg ccgtgccgta cgcgttcacc gccggcgctt gtgccctcgc catcgcctcc
     3181 gtgttgaaca cctcaacgat tcgtgaagtt gcgccgagcg atcccgtcgg gtcatgccgc
     3241 gtctcctccg acatcgcgta caccggcacc cggtcggcaa cgcgcgcacc ggagagaccg
     3301 agcgagatcg agtcggcaat cccgaggggc gcgaacacct cgtcgcgcat gaactctcgt
     3361 ggcaggcggc cgtcgatgcg gcgcaccacc tctcccaacg cccagccgta ggtgatcggg
     3421 tggtatccca cggcggcgcc gggcttccag gccgcggaga tggacgccgt ctgcgccgtc
     3481 acatacgccc agtcgtcgac gcggccccag tcgaaatcgt aagcgagctg tgggaagcct
     3541 ccctggtgag tcagcacgtg gcgcaccgtc gcggcgcgct tgccgccgtg cgcgaactgg
     3601 ggccagaacg aggccacggg ggtgtcatag gacagccgtc cgcgttcatg cagcaggtgg
     3661 acgcaggcgg cggcgatcgg cttcgtcgcc gagaacaaca gcatcgggtc gtcgcgtgtc
     3721 aggcggcggc cgggagcagc gaggccgccc gtgagttcga gggccagcac gccgtcgcgg
     3781 aacaccgcga gctgcgcccc cggatgccat ccaaccgcga gctgctgctc gaacagcgac
     3841 tccactcgcg cgatcccatc cgcgctcatc ccgcgggcgg aaggcgacgc cggagcggac
     3901 gtcattcgca agccgcccgc gccgcggcca caacgtcctc gatgcggcgc atgtagttcg
     3961 cggggtcctg cagcccgttc gtcacgtacg ccagggcgag accacgggac gggtcggcat
     4021 aagcaacggc gcactgctgc ccgccatgac cgaagacgcc cggcgtgcaa tacggttccc
     4081 aaataccgcc tgccatgaag cccaggccgt acgaggaggg caatccgctg gtgcggtcgg
     4141 ccacggcctc ggcgtgcacc cgggtcgcct cggcgaccgt acccgcttcg aggatgcgcc
     4201 tcccgccgag cgcgcccccg cgctcgagca tcgcgtagaa gtgcgccaac gcctcggccg
     4261 tcccgatggc gttc
//
LOCUS       DAORPG010000095         4270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1550_length_4270_cov_4.003796, whole genome shotgun sequence.
ACCESSION   DAORPG010000095 DAORPG010000000
VERSION     DAORPG010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1550_length_4270_cov_4.003796"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PLX85_03180"
     CDS             <1..246
                     /locus_tag="PLX85_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HRC62211.1"
                     /translation="SATEAEVAYDDPLYQRLRAWRLQRAREDGVPAYTLFSDRTAREL
                     ASRRPADEDALRSVWGMGDARVREFGRDLLAVITAED"
     gene            326..661
                     /locus_tag="PLX85_03185"
     CDS             326..661
                     /locus_tag="PLX85_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HRC62212.1"
                     /translation="MTLTMTVTESAAERLLAITAEHGITEEQGLRLFTRGGGCACSGP
                     SFGMAIDNAESGDSVFTLSGIRFIVDDVSADSLEGASIDWVDDVMRSGFTIEAPNAQT
                     GGGCGCGGH"
     gene            796..1680
                     /locus_tag="PLX85_03190"
     CDS             796..1680
                     /locus_tag="PLX85_03190"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HRC62213.1"
                     /translation="MHERLRLLGEVRPLRNPVLIAAITGWTDTNGAALSALEYLVEQW
                     DATPIADIDPEPFYDFTVQRPRTRLAGDDRVIDWPRNRFFTASPPGADHDFLLFGGIE
                     PHLRWRTFTEVVTSLMEAVGATTSVTLGVQPGAVPHTRALPVTLSASDNDFETQFGLK
                     IPGSRYEGPTGIISVLNLTFRARGWRNASLWAIAPHYLNTGPNPNAGISLVKVIDRGY
                     GTSTPLAGLEERSEEFAQQVQEAMEQSDEASTYIQTLEEQFDANAPETDESGGELPTS
                     DELLGDLERFLRQQREDR"
     gene            1749..1824
                     /locus_tag="PLX85_03195"
     tRNA            1749..1824
                     /locus_tag="PLX85_03195"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1782..1784,aa:Trp,seq:cca)
     gene            1835..1921
                     /locus_tag="PLX85_03200"
     tRNA            1835..1921
                     /locus_tag="PLX85_03200"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1869..1871,aa:Leu,seq:gag)
     gene            1953..2027
                     /locus_tag="PLX85_03205"
     tRNA            1953..2027
                     /locus_tag="PLX85_03205"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1985..1987,aa:Gly,seq:gcc)
     gene            2030..2103
                     /locus_tag="PLX85_03210"
     tRNA            2030..2103
                     /locus_tag="PLX85_03210"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2062..2064,aa:Cys,seq:gca)
     gene            2345..3574
                     /locus_tag="PLX85_03215"
     CDS             2345..3574
                     /locus_tag="PLX85_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC62214.1"
                     /translation="MDWSDTLEQAAFRAQVRSFIEQHLPDYYRKNYAEGHHVGPFSEN
                     FGGDRKSDDPVRRGSADDWARSLADRRWVAPHWPVEYGGAGMTAMEQFIFKQEMAAAG
                     APAVGGGGVQMLGPAMLVHGSEDQKREYLPKILSGEVGWAQGFSEPGAGSDLGSLQTR
                     AARDGDEYVVNGQKIWTSGAHDADWIFMLARTDPDAPKHRGISMLLFDIHTPGVSVRP
                     LISAGWQHDLNETFFEDVRVPAGQILGEENRGWYVAMTLLDFERSNIAGAVEARKELD
                     EVLDYVKTPAGRDIVQDRLDLTRHELAQRYIESEVLFNFSFRIISMQHQGMVPNTEAS
                     VSKIFGADLAQSVPRSVMKAFGPYANIWDREDSYAPLAASFTQLYVHTIPRSIMGGSS
                     EIQRGVIATRGLGLPRG"
     gene            3702..>4270
                     /locus_tag="PLX85_03220"
     CDS             3702..>4270
                     /locus_tag="PLX85_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008092590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HRC62215.1"
                     /translation="MASALFTPLALRDVEVRNRLWVSPMCQYSCDTDGLATDWHLVHL
                     GSRAVGGAGLVMAEATAVTPEGRISPYDLGIWSDAHVEALRPVTRFISAQGAVPAIQL
                     AHAGRKASTKRPWDGLGPVAPDEGGWETVGPSPIAYTEGYPVPRELSRDEIGGVVDAF
                     AQGARRAVEAGFRAIELHEAHGYLVAQFLS"
BASE COUNT          705 a         1524 c         1368 g          673 t
ORIGIN      
        1 agcgcgacgg aggccgaagt cgcctatgac gaccctctct atcagcgcct gcgcgcctgg
       61 cgactccagc gggcacgcga ggacggcgtc cccgcttaca ccctcttcag cgaccgcacg
      121 gcccgtgagc tcgcctcgcg ccgccccgcg gatgaggacg cactacgttc cgtctggggg
      181 atgggcgacg cccgcgtccg cgagttcggg cgggatcttc tggcggtcat caccgccgag
      241 gattgaccgc cgaatgtggt tgcggggact tgcccggaaa gcgcatgcta ggggtacgcc
      301 agcaacgcac aggcaggagt gagccatgac cctgaccatg accgtcaccg agagcgccgc
      361 cgaacgcctg ctcgccatta ccgccgagca cggcattacc gaggagcagg gactccgcct
      421 cttcacccgc ggcggcggct gcgcctgcag cggtccctcc tttggcatgg ccatcgacaa
      481 cgccgagagc ggcgacagcg tgttcaccct ctccggcatc cgcttcatcg tcgacgatgt
      541 ctcggccgac agcctcgagg gcgcctcgat cgactgggtc gacgacgtca tgcgctccgg
      601 cttcaccatc gaggccccga acgcgcagac tggcggtgga tgcggctgcg gaggccactg
      661 agcccgtccg caccactcat tggatcgggc gcgttcgagt gaacgcaccg caccggcccc
      721 ggcggcaagt ccggggccgg agtcgtgtct ttgccgctcg agcctgcccg ttcgcggact
      781 gctgaggtga ccttgatgca cgaacgtctc cgtctactcg gcgaagtgcg gcccctccgc
      841 aacccggtcc tgatcgctgc catcaccggc tggaccgaca ccaacggagc cgcgctctcc
      901 gccctcgagt acctcgtcga gcagtgggat gccacgccca tcgccgacat cgaccccgag
      961 ccgttctacg acttcaccgt gcagcgccca cgaacgaggc tcgccggcga cgaccgcgtc
     1021 atcgactggc cgcggaaccg cttctttacg gccagccctc ccggcgccga ccacgacttc
     1081 ctgctcttcg gcggtatcga gcctcacctg cgctggcgca cgttcaccga ggtggtgaca
     1141 agcctgatgg aggcggtcgg cgccacgacg agcgtcacgc tgggcgtgca gccgggtgcg
     1201 gtgccgcaca cgcgcgcgct gccggtcacc ctctcagcgt ccgacaacga ctttgagacg
     1261 cagttcggcc tcaagatccc cggcagccgc tacgagggcc ccacaggcat catcagcgtg
     1321 ctcaatctca ccttccgtgc gcgtggctgg cgcaatgcca gcctctgggc gatcgcacct
     1381 cactacctca acaccggccc aaacccgaac gcaggcatct ccctcgtaaa ggtcatcgac
     1441 cgcggctacg gcacctccac gccactcgcc ggcctcgagg agcgcagcga ggagttcgcg
     1501 cagcaggtgc aggaagcgat ggagcaatcc gacgaagcct cgacgtacat ccagacgctc
     1561 gaggagcagt ttgacgccaa cgctcccgag accgacgagt caggaggcga gcttcccact
     1621 agcgacgagc tgcttggcga cctcgaacgc ttccttcgcc aacagcgaga ggaccgctag
     1681 ccccggaggg tcgctcgaac agccgccttc accctcacgc gtggcgttgc tacaatcgga
     1741 agccgcgcag gagtgtagct cagttggcag agcggcggtc tccaaaaccg taggccgggg
     1801 gttcgaatcc ctccactcct gccatcgttg cagtgccgag gtggtggaat aggcagacac
     1861 gccgtcttga gggggcggtg ctcgcaaggg cgtatgagtt caagtctcat cctcggcacc
     1921 aacaacaccg ccggttcgcc ggcaacaggt cagcggaagt agctcagtgg tagagcgcct
     1981 ccttgccaag gaggaggtcg cgagttcgac cctcgtcttc cgctccaggg gccacgtagc
     2041 caagtggcaa ggcagaggtc tgcaaaacct ccatcatggg ttcgattccc atcgtggcct
     2101 ccaagtcccc acctccccct cctcgacgct ctggtcggca tgcctcccgg actccgtccg
     2161 cggctgatcc tggccgccgg cgccgcggca ggtctttgac cgactgagct cgcgcccgct
     2221 gcgcctgcag ccaggcctcc ccacgtttga gctctggatc gaggacagca cgctccattc
     2281 gtccaccata tccgttatcg tggtgcccgc gacggcgcgg cgggcgccgt gaggaggtag
     2341 ccgcatggac tggtccgaca ctctcgagca agccgcattc cgggcgcagg tgcgatcctt
     2401 catcgagcag cacctccccg actactaccg caagaactac gccgaggggc atcacgtcgg
     2461 gccgttctcg gaaaacttcg ggggcgaccg caagtccgac gaccccgtgc ggcgagggtc
     2521 ggccgacgac tgggcgcgtt cgctcgccga ccgccggtgg gtggcgccgc actggccagt
     2581 tgagtacggc ggcgcgggca tgacggccat ggaacagttc atcttcaaac aggaaatggc
     2641 cgccgccggc gcgcccgccg tgggtggcgg cggcgtccag atgctcggcc ccgccatgct
     2701 cgtccacggc tccgaggatc agaagcgcga atacctcccc aagatccttt ccggtgaggt
     2761 cggctgggcg cagggcttct cggagccggg agcaggctcc gacctcgggt cgctgcagac
     2821 ccgtgcggcc cgcgatggcg acgagtacgt cgtcaacggg cagaagatct ggacctcggg
     2881 cgcgcacgat gcggactgga tcttcatgct cgcccgtacc gaccctgacg cgccgaagca
     2941 tcgcgggatt tcgatgctgc tcttcgacat ccacaccccg ggcgtctcgg tgcggccact
     3001 catctcggcc gggtggcagc acgacctcaa cgagaccttc ttcgaggacg tccgcgtccc
     3061 cgccggacag atcctcggcg aggagaaccg cggctggtac gtggcgatga cgctgctcga
     3121 cttcgagcgc tcgaacatcg cgggggcggt cgaagcacgc aaggaactcg acgaggtgct
     3181 cgactacgtg aagacgcccg ccggccgcga catcgtgcag gaccgcctcg acctaacccg
     3241 ccacgagctg gcccagcgct acatcgagtc cgaggtgctg ttcaacttct ccttccgcat
     3301 catctcgatg cagcaccagg gcatggtccc gaacaccgag gcgtccgtct ccaagatctt
     3361 cggcgccgac ctcgcgcagt ccgtcccgcg gtcggtgatg aaggccttcg gcccgtacgc
     3421 gaacatctgg gaccgcgagg actcgtacgc ccccctcgcc gcctcgttca cgcagctcta
     3481 cgtgcacacc atcccgcgct cgatcatggg cggctcctcc gagatccagc gcggcgtcat
     3541 cgcaactcgc gggctcggac tgccgcgcgg ctgatccggc gacgcgtctc atcacccgga
     3601 gggcagggct gtgccccgcc ctctcgcgtc gtggtcgtgc gtacacacgc gcatgccata
     3661 ctcaggcgaa ccgtacgaat ccgagatacg aggagcagtc gatggcgtcc gctctcttca
     3721 ccccgctggc actgcgcgat gtcgaagtcc ggaaccggct ctgggtgtcc cccatgtgcc
     3781 agtactcttg cgacaccgat ggactcgcga cggactggca tctcgtgcac ctcgggtcac
     3841 gggccgtcgg cggcgcggga ctggtgatgg ccgaggcgac tgcggtgaca ccggagggac
     3901 gcatctctcc gtacgacctc gggatctggt ccgacgcgca tgtcgaggcg ttgaggccgg
     3961 tcacgcggtt catttcggcg cagggagccg tccccgcgat ccagcttgcg cacgcgggcc
     4021 ggaaagcctc gacgaaacgg ccctgggacg gcctcgggcc ggtggctccc gacgagggtg
     4081 gatgggagac cgtcggccca agcccgatcg cctacaccga gggctatccc gtgcctcgcg
     4141 agctttcgcg cgacgagatc gggggcgtgg tggacgcctt cgcgcaggga gcacgccgcg
     4201 ccgtcgaggc gggcttccgc gcgatcgagc tgcacgaggc gcacggctac ctcgtggcgc
     4261 agttcctctc
//
LOCUS       DAORPG010000096         4270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1551_length_4270_cov_3.813998, whole genome shotgun sequence.
ACCESSION   DAORPG010000096 DAORPG010000000
VERSION     DAORPG010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1551_length_4270_cov_3.813998"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PLX85_03225"
     CDS             <1..709
                     /locus_tag="PLX85_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HRC62216.1"
                     /translation="DEVLAEGLAPGDLEALLSQRRRWAVGALQVLRREAPLTARGLSV
                     GQRLMYLETVLRYFSGFATATLAIVTALALTFGWWPVDSYDASLLARAIPLALANEVL
                     LLTAMRGPGAVRERQLSIALWPVWIEAACTALAPARAPATPSFEVTPKDGATRRASLK
                     LLRWQLGAAALLIGSVLVAGIRLAAGVEDDLNAVVVHLAWAAYLLGGLAMLPAAWAYR
                     RNAVPSSAPALEREVPR"
     gene            complement(706..1548)
                     /gene="arsM"
                     /locus_tag="PLX85_03230"
     CDS             complement(706..1548)
                     /gene="arsM"
                     /locus_tag="PLX85_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HRC62217.1"
                     /translation="MTTDAETIRDAVRGAYGERAREVLSGAPGDRAGGVASKLYAEAD
                     SLPAGVVSYGCGNPVAIAQLQAGETVLDLGSGAGLDCFLAARQVGPSGRVIGVDMTDD
                     MLTLAETNRARLLAAGDHVANVEFRRGLIEAIPLDDAAVDVIISNCVINLSTDKDAVF
                     SEAFRVLRPGGRFQVSDVVLLREISNEERADLDLWSGCKSGALHLDDYRSRLAAAGFE
                     DVEIAVEANPTDAWASALINARRPGGGAPRRGWQFSGPTIELLAPAGLDTADCCSLDD
                     PSCC"
     gene            1683..2591
                     /locus_tag="PLX85_03235"
     CDS             1683..2591
                     /locus_tag="PLX85_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HRC62218.1"
                     /translation="MTGLVRLAGLCAVFATMLLALSCGGGTESQSDVVATTVQIGALT
                     REVAGDRLTVHILVGPGVDPHDFEASANDLRQISGAKLVLRNGIGLDDFLDRAMKSGG
                     ARQVVTVTDGITVRRAADSDGGRGDEDPHVWHNPLNAKVMVDDIVRALSDAYPEDAAT
                     FRANGDSYKATLDETDAEIRRLIDTIPPANRKMVTNHDAFGYFIERYGLTYVGAVIPG
                     LSTQGEPSSKEIATLEDTIRRESVKAIFAESSVDPKVAREIARDTGVRIVDDLYGDSL
                     GDPGTEAATLDGMLLANARKIADALR"
     gene            2588..3331
                     /locus_tag="PLX85_03240"
     CDS             2588..3331
                     /locus_tag="PLX85_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HRC62219.1"
                     /translation="MSQQALANGPLRLDHVAVGYDSTFVLSDVSFEVQPGDVVGVIGP
                     NGGGKSTLLKAIARVLPLRAGHVTLAGRGIEEYAGRIAFVPQREEVNWDFPVTALDVV
                     LMGRYRGAGWLRRPSRADRERAAEALERLGLGGMGGRHISQFSGGQQQRIFLARALVQ
                     DPLVVLLDEPFTGIDAANRAVFHEAIAAFARQQVIVLMATHDLEEMAATCSYVCCVNQ
                     RLVAFGPTRYTYTPEVIRETFAGRLAVFS"
     gene            3328..4227
                     /locus_tag="PLX85_03245"
     CDS             3328..4227
                     /locus_tag="PLX85_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRC62220.1"
                     /translation="MIGWVTDPLQYPFIQRALIEIAIVGALCGLLGCFVVLRGLAFIG
                     DALAHAVFPGVVLSYLAGRSILIGAFVFGGLTALGIGVLSRSRRVSEDTAIGVIFAAF
                     FALGVVLLSRQTGFRRDLGSLLFGSILGVSTQDVVVSALIAAVVVVVLFALMKEFTLV
                     AFDVTLARSLGYPVFLLDLVLLLLVSATIVVSLQTVGNILILALIVTPPATARLLTDR
                     LGRMLAISATTGVASGVAGLYISYHAGTAAGGTIVLTATACFLVALVLAPNHGLLSAR
                     LQRRRGRHHVHHYHPAGEDTGPA"
BASE COUNT          633 a         1508 c         1400 g          729 t
ORIGIN      
        1 cgacgaagtg ctcgcagaag gccttgcgcc gggcgacctc gaggcgctcc tttcgcagcg
       61 ccggcgatgg gcggtcggcg cactccaggt cctgcgccgg gaagccccct tgaccgcgcg
      121 agggctcagc gttggccagc ggttgatgta cctcgagacg gtgctgcgct acttctccgg
      181 attcgcaacc gccacgcttg cgatcgtcac ggcgctggcg ctcacattcg gctggtggcc
      241 ggtcgactcc tacgacgcga gtctcctggc gcgggcgatt ccgcttgcgc tcgctaacga
      301 ggtgttgctg ctgacggcga tgcgcgggcc cggtgccgtc cgtgagcggc agctctcgat
      361 cgccctctgg ccggtctgga tcgaggccgc atgcaccgcg ctcgcaccgg cgcgtgcccc
      421 tgctacgcca tcattcgagg tgactccaaa ggatggcgca acgcgccggg catcgctgaa
      481 gctcctgcgc tggcagctcg gcgcggctgc gctgctcatc gggagcgtcc tggtcgcggg
      541 gatccggctg gccgcgggcg tcgaggacga cttgaacgcc gtggtcgtgc acctcgcctg
      601 ggcggcttac ctgctcggtg ggctggctat gctgcccgcc gcgtgggctt atcggcgcaa
      661 cgccgtgccg tcgtcagcac cggcactcga gcgagaggtg ccccgctagc agcaggatgg
      721 gtcgtcgagg ctgcagcagt ccgccgtgtc gaggcccgcc ggcgcaagca actcgatcgt
      781 cggcccgctg aactgccacc cccgccgagg ggcgccccct cccgggcggc gagcgttaat
      841 gagcgcgctc gcccaggcgt ccgtcgggtt ggcttccacg gcgatctcga catcctcgaa
      901 ccccgcagcg gcaaggcgag accggtaatc gtcgaggtgg agcgccccgg acttgcaacc
      961 agaccacagg tcgaggtccg cgcgctcttc gttgctgatc tcgcgcagca gtacgacatc
     1021 ggacacctgg aagcggccgc ccggccgcag cacgcggaat gcctcgctga agacggcgtc
     1081 cttgtctgtc gacagattga tcacgcagtt cgaaatgatt acgtcgaccg ctgcgtcatc
     1141 gagcggaatg gcctcgatca gtccacgccg gaactcgacg ttcgcaacgt gatcgcccgc
     1201 agccagcagc cgcgcccggt tcgtttcggc gagcgtcagc atgtcgtcgg tcatgtcgac
     1261 gccgatcacg cgcccgctcg gccccacctg ccgcgctgca aggaagcagt cgaggcccgc
     1321 gccggaaccg aggtcgagca cggtctcacc cgcctgtagt tgagcgatcg cgacgggatt
     1381 cccgcagccg tacgacacca cgcctgccgg caacgagtcc gcctccgcgt acagcttcga
     1441 cgccacacca ccggcacgat cccccggcgc tcccgacagc acctcgcgtg ctcgctcgcc
     1501 gtaggcgccg cgtacagcgt cccgaatcgt ctcggcatcg gtggtcatga ccgtctccct
     1561 ccgcgttgtc ttcgcatggt actcgctcgt gccgccaccg agggtggccc cgaccagcgc
     1621 ttattgacac tttgtctcat ctcatctata ggtaccaggt atcaattggg aaggtgtgca
     1681 ccgtgacagg cctggtccgc ctcgccggcc tctgcgccgt cttcgcgacg atgctccttg
     1741 ccctcagctg cgggggcggg acggagagcc agagcgacgt cgtggcgacg acggtgcaga
     1801 tcggtgcact gaccagggag gtcgccggcg accgcctcac cgtgcacatc ctcgtcgggc
     1861 cgggcgtcga cccgcacgac ttcgaggcaa gcgcgaatga cctgcggcag atctcagggg
     1921 ccaagctcgt gctgcgcaac ggcattggtc tcgatgactt cctcgaccgc gcgatgaaga
     1981 gcggaggcgc gaggcaggtg gtgacggtca ccgatggcat cacggtgcgc cgggctgccg
     2041 actccgatgg aggccggggt gacgaagacc cccacgtctg gcacaacccc ctcaacgcga
     2101 aagtgatggt cgacgacatc gtgcgcgcgc tctcagatgc ctatcccgaa gatgccgcga
     2161 cattccgcgc aaacggggac tcctacaagg caacgctcga tgagacggac gcggaaatcc
     2221 gtcgcctcat cgacacgatc ccgccggcga atcgcaagat ggtcacgaac cacgacgcct
     2281 tcggctactt catcgagcga tacggcctga cctatgtcgg cgccgtgatc ccgggcctct
     2341 ccacccaggg cgaaccgtcc tccaaagaga tcgccaccct cgaggacacc atccgccgcg
     2401 aaagcgtgaa ggcgatcttc gccgaaagtt cggtcgaccc gaaggtcgcc cgcgagattg
     2461 cgcgcgacac cggcgtacgc atcgtcgacg acctctatgg cgactcgctg ggagacccgg
     2521 gcacggaggc cgcgacgctg gacggcatgt tgctggcaaa cgcccgcaag atcgcggacg
     2581 cgctgcgatg agtcagcaag cgctcgccaa tggcccgctc cgcctcgacc acgtagccgt
     2641 cggctacgac tcgacgttcg tgctttcgga cgtgagtttc gaggtccaac caggcgacgt
     2701 ggtcggtgtg atcgggccga atggcggcgg aaagagcacg ctgctcaagg ccatcgcgcg
     2761 cgtccttccg ctgcgcgccg ggcacgtcac gctcgccggc cgcggtatcg aggagtacgc
     2821 ggggcgcatc gccttcgtgc cgcagcggga ggaagtcaac tgggactttc cagtgaccgc
     2881 gctggacgtc gtcctgatgg gtcgctatcg aggcgcgggc tggctacggc gtccctcacg
     2941 agcggaccgc gagcgtgcgg ccgaggcgct ggagcgcctg ggcctcgggg gaatgggtgg
     3001 ccgccacatc agccagttct caggcggcca gcagcagcgc atctttctcg cgcgagcgct
     3061 cgtgcaggat ccactcgtcg tactcctcga tgagccgttc accggcattg acgccgccaa
     3121 ccgcgccgtc ttccacgagg caatcgccgc cttcgctcgc cagcaggtga tcgtgttgat
     3181 ggccacgcac gacctcgagg aaatggcggc cacctgctcg tacgtctgct gcgtgaacca
     3241 gcggctggtc gcgttcggac ccacgaggta cacctacacc cccgaggtca tcagggagac
     3301 cttcgcgggc cggttggcgg tgttctcgtg atcggctggg tgaccgaccc gctgcagtac
     3361 ccgttcatcc agcgggcgct gatcgagatc gcgatcgtgg gtgcgctctg cggcctgctg
     3421 ggctgcttcg tcgtcttgcg tggcctcgcg ttcatcggcg atgcgctggc gcacgccgtg
     3481 ttcccgggcg tcgtgctctc ctacctggcc ggacgcagca tcctgatcgg cgcgttcgtc
     3541 ttcggggggc tgaccgccct cggcatcggc gtgctctcgc ggagccgtcg cgtcagcgag
     3601 gacacggcca tcggcgtcat ttttgccgcc ttctttgcgc tcggcgtcgt cctgctcagc
     3661 cgccagaccg ggttccgtcg cgaccttggc tcgttgctgt ttggcagcat cctgggcgta
     3721 tccacgcagg acgtcgtggt ctcggcgctg atcgccgccg tcgtcgtggt cgtgctcttc
     3781 gcgctgatga aggagttcac cctcgtcgcg ttcgacgtca cgctcgcgag gtcgctcggc
     3841 taccccgtgt tcctgctcga cctcgtgctg ctgctgctcg tgtcggccac gatcgtcgtg
     3901 agccttcaga cggtcggcaa catcctcatc ctcgcgttga tcgtgacccc gcccgccacg
     3961 gctcggctgc tcaccgaccg cctcggccgc atgctcgcca tcagcgcgac gactggggtc
     4021 gcgagtggcg tcgccggtct ctacatctcg taccacgccg ggaccgctgc cggcggcacg
     4081 atcgtgctga cggccactgc ctgcttcctc gtggccctgg tgctcgcgcc caaccacgga
     4141 ctgctcagtg cgcggctcca gcggcgacgc gggcgtcacc acgtccatca ctaccacccg
     4201 gccggcgagg acaccggccc cgcctgacga cggcgtcggt gacgccgttc cagcgcacga
     4261 gaccgccacg
//
LOCUS       DAORPG010000097         4259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1555_length_4259_cov_3.638915, whole genome shotgun sequence.
ACCESSION   DAORPG010000097 DAORPG010000000
VERSION     DAORPG010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1555_length_4259_cov_3.638915"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PLX85_03250"
     CDS             <1..496
                     /locus_tag="PLX85_03250"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC62221.1"
                     /translation="RDYEWRRDPELARFDAARPLTMSYRAFVSSMSEDLRFTSAYRRS
                     FAIEELDAGTHIGNVMYYGYDPAAREAELGITIGDRDYWSRGYGRDAVSVLVRYLFEE
                     RDLARVYLHTLSWNYRAQAAFRHAGFHHVRAVHRDGHDFEQMEIRPEDLLDGASGDGL
                     AGGQ"
     gene            607..978
                     /locus_tag="PLX85_03255"
     CDS             607..978
                     /locus_tag="PLX85_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRC62222.1"
                     /translation="MPEQFGRVGVLLMFALLFPAIPLIASYAFYRFRIRPEHHDPIKD
                     ATYECGVESEGSAWGQFHARYYLFALGFVIFDVEVVFLYPWAVVHGGLGFTALFEAVL
                     FIGILALGLGYAWRRRALEWR"
     gene            979..1494
                     /locus_tag="PLX85_03260"
     CDS             979..1494
                     /locus_tag="PLX85_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRC62223.1"
                     /translation="MPRGPRQLEGHTVAGRLRRSLPQAVVAETDEWVEVAPDAIVDVV
                     RWLHDDPDLDAAQLSNLCATDRYDHFEVVYHLQSLDQNHQIVVKARVGEHERASLPSV
                     YPVYKGALLQEREVYDLMGVRFEGHPDLRRMFLWEGYPGFPLRKDFLQITGHHPGLPH
                     FPFEEAGRQER"
     gene            1491..2594
                     /locus_tag="PLX85_03265"
     CDS             1491..2594
                     /locus_tag="PLX85_03265"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRC62224.1"
                     /translation="MTTASEPYVLNIGPQHPSTHGVFRLRVVMDGERIQDIDMVVGYL
                     HRSMEKLAEERTYTQNIPFTDRTDYLAAMSNNLGYCLAAERLAGIEVPDRASYLRVIF
                     AELQRLASHSMANGTFINDAGAWQTPLMYMFREREKILDLFEMTCGARLTTNYMRIGG
                     VAFDPPAEFFPAVNALIDELPDRIAEYTELLVDNEILLARSRGVGVVSAEDAINGSLV
                     GPVLRASGVPWDLRKAEAYCAYGDFDFEIPVGHVGDCYDRFMVRLEEMRQSVRIIRQA
                     LDGLPSGPHKTEIPLALRPAPGEAYARVEGPRGELGFYVVSDGGPAPFRFHIRAPSLI
                     NLSLLREMAVGTSLPDAIVTLGSLDIVVGEIDR"
     gene            2594..4015
                     /gene="nuoH"
                     /locus_tag="PLX85_03270"
     CDS             2594..4015
                     /gene="nuoH"
                     /locus_tag="PLX85_03270"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRC62225.1"
                     /translation="MRSAWLDPVREPTRRLSPQVLAPLAVLLVAFLALNVAIAVLIVS
                     GRLDGHWYDFRDLGNAVQALREWLDDRTADQPGWLSDLLGDHWLSYIVTALIGAIGIL
                     SFVGVVLLVLIWVERRLIGRIQVRRGPNRVGPFGLLQPFADAIKLIQKETLIPRGADR
                     FMFYLPPVLVFIPGLLVWAPVPWAPHMSYLDINVGVLYLIAISSLSTLAIFMAGWSSN
                     NHYALLGAMRTVAMMISYEIPLSIALLSVVLFTGSMQLSEIVQWQADHNMWLGVLMPL
                     ALFTFFFSSTAELNRTPNDIAEAESEIVAGFHTEYSGMKFGLFYAVELGNAVAVAAFV
                     GTFFLGGWSLFGLEEWVPPYLVFALKIAAGYFVLVWLRGTLPRFRLDQLMMFAWKYLI
                     PISLLQVILVAFERSIFEHWDVPGVIPLALFTIVNAVFAVRIVRFWARFLGYRPERET
                     LLAPTLTRSVGGLQAAQRMRTVR"
     gene            4015..>4259
                     /locus_tag="PLX85_03275"
     CDS             4015..>4259
                     /locus_tag="PLX85_03275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRC62226.1"
                     /translation="MESTGIVLGFWLLSGVTVASALLVFLSRNLLHAVLALVLSFLGM
                     AGLFVTLSADFIAVAQVLIYAGAISVLLVFAVMLTPLA"
BASE COUNT          602 a         1393 c         1394 g          870 t
ORIGIN      
        1 ccgcgactac gaatggcggc gcgatcccga gctcgcccgt ttcgacgcgg cgcgtccgct
       61 caccatgtcg tatcgcgcgt tcgtctcctc gatgtccgag gacctgcggt tcacgagcgc
      121 ctaccggcgc tcgtttgcca tcgaggagct cgatgcgggg acgcacatcg gcaacgtcat
      181 gtactacggc tacgatcccg cggcgcgcga agcggaactc ggtatcacca tcggggaccg
      241 cgactactgg tcgcgcggct acggacggga cgccgtgagc gtcctcgtgc gctatctctt
      301 cgaggaacgc gacctggccc gcgtgtactt gcataccctc tcctggaact accgcgctca
      361 agcggcgttc aggcatgccg ggttccacca cgtccgggcg gtgcaccgcg acggccacga
      421 cttcgagcag atggagatcc ggccagagga tctcctcgat ggggcctcgg gcgacggctt
      481 ggcgggtgga cagtagcccc tcgggcgtat tcgcttcgtt caattgcgcc catctggggc
      541 gcgtgctaca acgaatccgg ccctcgtcgg ggcgccagcc tgtgctgctg agggagccgg
      601 cgcgtgattc cagagcagtt cggacgcgtt ggcgtcttgc tcatgttcgc gcttctcttc
      661 cccgcgatcc cgctgatcgc gtcgtacgcc ttctaccgct ttcgcatccg tcccgagcac
      721 catgacccca tcaaggacgc cacctacgag tgcggcgtcg agagtgaagg cagcgcctgg
      781 gggcagttcc acgcgcgata ttacctgttc gcccttgggt tcgtaatttt cgacgttgaa
      841 gttgtgttcc tctatccgtg ggcggtcgtc catggcgggc tcggattcac tgccctgttc
      901 gaggcggtct tgttcatcgg catccttgct ctgggcctcg ggtacgcctg gcgtcgccgc
      961 gcgctggagt ggcgctaatt gccccgcggg ccgcgtcagc tcgagggcca cacggtggcc
     1021 ggtcggctcc ggcggtcgct gccgcaggcg gtggttgccg agaccgacga gtgggtcgaa
     1081 gtcgcccccg acgccatcgt cgatgtagtg cgctggctgc acgatgaccc tgacctcgat
     1141 gccgcgcagt tgagcaacct ctgcgccacc gaccgctatg accatttcga ggtggtctat
     1201 cacctgcagt cgctcgacca gaatcaccag atcgtcgtga aggcgcgggt tggcgagcat
     1261 gaacgcgcgt cgctgccgag cgtgtacccg gtgtacaagg gggcgctgct gcaggagcgc
     1321 gaggtgtacg acctcatggg cgtccgcttc gagggccacc ccgacctgcg acggatgttc
     1381 ctgtgggaag gctaccccgg gttcccgttg cgcaaagact tcttgcagat cacgggccat
     1441 catccaggcc tgccccactt cccattcgaa gaggcaggac ggcaggagcg atgacgaccg
     1501 cgagtgagcc ctacgtcctg aacatcggtc cgcagcatcc ctcgacgcac ggcgtgttcc
     1561 gcctgcgcgt cgtgatggac ggcgagcgga ttcaggacat cgacatggtg gtgggctacc
     1621 tgcaccgctc gatggagaag ctcgctgagg agcggacgta cacgcagaac atcccgttca
     1681 ccgaccgcac ggactacctc gcggcgatgt ccaacaatct gggatactgc ctcgcggccg
     1741 agcggctggc gggcatcgag gtgcccgacc gcgcttccta cctgcgggtg atcttcgcgg
     1801 agttgcagcg cctcgcgagc cactccatgg cgaacggcac gtttatcaac gacgccggcg
     1861 cgtggcagac gccgctcatg tacatgtttc gcgagcgcga gaagatcctc gacctcttcg
     1921 agatgacctg tggcgcccgg ctgacgacga attacatgcg catcgggggc gtcgcgtttg
     1981 acccgcccgc cgagttcttt cccgcggtca acgcgctgat cgacgagctg cctgaccgta
     2041 tcgcggagta caccgaactg cttgtcgaca acgagatcct gctggctcgc tcgcgcggcg
     2101 tgggcgtcgt gtctgccgag gacgccatca acggctcgct ggtcgggccg gtgctgcgcg
     2161 cttcgggtgt gccgtgggat ctccggaaag ccgaggcgta ctgcgcctac ggagacttcg
     2221 acttcgagat cccggtcgga catgtgggtg attgctacga ccgcttcatg gtgcgtctcg
     2281 aggagatgcg ccagtcggtc cgcatcattc ggcaggcgct cgatggtctg ccgtccgggc
     2341 cgcacaagac cgagataccg ctcgccctgc gccccgcccc gggagaggcg tacgcccggg
     2401 tcgaaggtcc ccgaggggag ctgggcttct acgtcgtgtc cgacggcggc ccggcgccct
     2461 tccgcttcca catacgggct ccatcgctca ttaatctgtc gttgctccgt gagatggccg
     2521 tcggtacttc gctgccggac gccattgtca cgctcggatc gcttgacatt gtggtcgggg
     2581 agatcgaccg atgatgcgca gcgcgtggct tgacccggtg cgcgagccga cgcggcgtct
     2641 gtcgccgcag gtgctcgcgc ctctcgcggt cctgcttgtc gccttcctgg cgctgaacgt
     2701 tgccatcgcg gtcctgatcg tcagtggacg cctcgacggt cactggtacg acttccgcga
     2761 cctgggcaat gccgtccagg cgctgcgaga gtggctcgac gaccgcaccg ccgaccagcc
     2821 agggtggctg tccgacctgc tgggtgacca ctggctgtcg tacatcgtca ccgcgctcat
     2881 cggcgccatc ggcatcctgt ctttcgtggg cgtcgtgctc ctcgtgctca tctgggttga
     2941 gcggcggctc atcgggcgca tccaggtgcg ccgaggcccg aaccgggtgg ggccgttcgg
     3001 cctgcttcag ccgttcgccg acgcgatcaa gctgatccag aaggaaacgc tgatcccgcg
     3061 cggcgcggac cgcttcatgt tctatctgcc gccggttctg gtgttcatac ccggcctgct
     3121 ggtctgggcg cccgtgccct gggcgcccca catgtcgtac ctcgacatca acgtcggcgt
     3181 gctttacctg attgcgatca gttcgctgtc gacgctcgcc atcttcatgg cgggctggtc
     3241 gtcgaataac cactacgcgt tgctgggcgc gatgcgcacg gtcgcgatga tgatctcgta
     3301 cgagatcccg ctgagcatcg cgcttctgtc ggtggtgctc ttcaccggct cgatgcagct
     3361 gtccgagatc gtgcaatggc aggcggacca caacatgtgg cttggcgtgc tgatgccgct
     3421 cgccctgttc acgttcttct tctcctcgac cgccgaactg aaccggacgc cgaatgacat
     3481 cgccgaggct gagtcggaga tcgtagccgg gttccacacc gagtactccg ggatgaagtt
     3541 cggcctcttc tacgcggtcg agctggggaa cgcggtcgcg gtggcggcgt tcgtggggac
     3601 gtttttcctc ggcggctggt cgctgttcgg cctcgaggag tgggtgccgc cgtatctggt
     3661 ctttgctctg aagatcgcgg cggggtactt cgtgctggtc tggctgcgcg gtacgctgcc
     3721 gcgcttccga ctggaccagc tgatgatgtt cgcctggaag tacctgatcc cgatttcgct
     3781 gttgcaggtc atcctcgtag cgttcgagcg ctcgattttc gagcactggg acgtgccggg
     3841 cgtcatcccg ttggccctgt tcacgatcgt caatgcggtc ttcgccgtgc ggatcgtccg
     3901 cttctgggcg cgcttcctcg gctaccggcc ggagcgcgag acgctcctcg cgccgacgct
     3961 tacgaggagc gtgggcggtc tgcaggccgc gcaacgaatg cggacggtgc gctagtggag
     4021 tcgaccggga ttgtcctcgg cttctggctg ttgagcggcg tcaccgtcgc cagcgcgttg
     4081 ctggtcttcc tgtcgcgcaa cctgctgcac gccgtgcttg ccctcgtgct gagtttcctc
     4141 ggcatggccg ggttgttcgt cacactgtcc gctgacttca tcgcggtagc gcaggtgctg
     4201 atctacgccg gcgccatctc ggtgctctta gtcttcgccg tgatgctcac accgctggc
//
LOCUS       DAORPG010000098         4242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1568_length_4242_cov_3.407929, whole genome shotgun sequence.
ACCESSION   DAORPG010000098 DAORPG010000000
VERSION     DAORPG010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1568_length_4242_cov_3.407929"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLX85_03280"
     CDS             complement(<1..222)
                     /locus_tag="PLX85_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRC62227.1"
                     /translation="MAGPLAGIRVIDLSSGQAAGLATMVLADFGADVVKVEPSAGDPA
                     RAEPAAPMWLRGKRSLTLDLATAEGRDRLH"
     gene            complement(311..697)
                     /locus_tag="PLX85_03285"
     CDS             complement(311..697)
                     /locus_tag="PLX85_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62228.1"
                     /translation="MIELAARLTVEPAEATVPGDFTARCELHNVGDEEAAINAAPLSS
                     PSLALEVVDAAGELVLLPPPPVPPAVPPVERLGVGEVRVAEFPGFLPAWTPPGRYRVR
                     FRYLGSGPGLFAGLIVSTAVEFTLRA"
     gene            complement(700..1863)
                     /locus_tag="PLX85_03290"
     CDS             complement(700..1863)
                     /locus_tag="PLX85_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62229.1"
                     /translation="MPQLEAAIRVEKPEEVVPGEFTLTVVFTNRSSTPASLNTHQAEH
                     PALVLDVRDASDRPVLLPPPSAPDAADLAPGEPIVPGGSLSLTYVGFLDRGLPPGEYR
                     VRYFGEFPALGGSRDDPLRSDWQTFTVRAQRDLPPGLEVPGLKPGPEGPEEKPLLPLP
                     RLLGPVWLYVVRLRCWVWCLLLRWVFLRRCDRVLQQEIDQARSETISNAPPGSEAWNG
                     TYGWHARFLLIADEPNCRVTARVRVRLVGTITPAQRTAWEAAIEAAWNHRFKLCCDCC
                     CPSGMDIVCDIEFVNSGEHQVVNVGTSTTNMSHWGAADTIDVSHEFGHMLGALDEYFT
                     VNGVNYGAGRQPGGAIMNNPANPPAARHYDLVRAGAAALLGHSCSTADVGASC"
     gene            complement(2026..>4242)
                     /locus_tag="PLX85_03295"
     CDS             complement(2026..>4242)
                     /locus_tag="PLX85_03295"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRC62230.1"
                     /translation="AVAGGAVRHPWLTVLLWVMLLGAAFVSAGSLGDVLSDNNNVSGS
                     SEAARAQRLIDGPLADPNAPGAHEYVLVEAQGHASADSMRQTVASVAEQLRGTANVRA
                     VHTYLDGEAGFITDDGQVAMLIVDINGDSNEAKTLAEPVVATVASADTAADGYRITTV
                     GEGSVNAEFEDLAKQTLERGEMVGLGIGLVILLVVFGAAVAAGLPVLLALASIVLALG
                     TTALVGRALDLSTFVTNIITMIGLAVGIDYSLFIVQRFREERSLGLARDEAIRTAAAT
                     ATRAVFVSGIAVVIALSGLLIMPDATFRAFGIGAILVVIAAVLAALTLLPAVLRLLGD
                     RVNWLTLPVIGRRRSPENEGGVWGFTTRLVTARPVFSVIVAGGLLLGAASAVGWMQLG
                     SNGISSLPETSNGRHAFEVLTSRFDSAAVITADLVIQARDVHDPAVQSAIERFEGLAA
                     ADSFYGAPQLRTDAAGDLALLSLPVLDDPASAQARDALDRVRDDYIPQAFSGSGATVL
                     VGGATATEVDSVAEIRHYTPIVFGVVLGFSFLLLTMVFRSIVVPLKAVAMNLLSVGAA
                     YGMIVLVFQKGIGAERLGFHQMPVIESWLPLFLFSILFGLSMDYHVFLLSRIKERYDE
                     TGDNRHAVEHGLRSTGSIITGAALIMVSVFGGFALGDLAMFQQMGFGLAVAVIVDATI
                     VRSVLVPATMELLGDWNWYFPGWLAWLPNLEIEGRRPAAQSTRTPAVLAEAGDGGA"
BASE COUNT          701 a         1336 c         1626 g          579 t
ORIGIN      
        1 gtggaggcgg tcgcgcccct cggcggtggc aaggtcgagt gtgaggctgc gcttgcctcg
       61 gagccacatg ggggccgcgg gctcggcgcg ggcggggtcg ccagcggacg gctcgacttt
      121 gacgacgtct gcgccgaagt cggcgagcac catggtggcg aggcctgcgg cctggccgct
      181 gctgaggtcg attacgcgga ttcccgcgag tggtccggcc atgtgcgttc gccctccctc
      241 ggtgcgcacc acagcggcgg ggtacagggg gcgagcatag cgcctcgcga cgcggcgcga
      301 cgcccgcccg tcaggcccga agcgtgaact cgacggcggt ggatacgatg aggcccgcga
      361 agaggccggg gccgctgccg aggtagcgga agcgtacccg gtagcggccc ggcggcgtcc
      421 aggcgggaag gaagccgggg aactcggcga cgcgcacctc gcccacacca aggcgctcga
      481 ccgggggcac cgccggtggc accggcggtg gcggcaggag cacgagctcg ccggccgcgt
      541 cgaccacctc gagggcgagg gagggtgacg agagcggcgc cgcgttgatg gcggcttcct
      601 cgtcgccgac gttgtggagc tcgcagcgcg cggtgaagtc gccggggacg gtggcctcgg
      661 cgggttcgac cgtcaggcgc gcggcaagct cgatcaccgt cagcagctcg caccaacgtc
      721 ggcggtggag cagctgtggc ccagcagagc ggccgcgccg gcgcgaacca ggtcgtaatg
      781 cctcgcagca ggcggattcg cagggttgtt catgatggcg cccccgggct gccgacctgc
      841 cccatagttg actccgttta ccgtgaagta ctcgtcgagc gcgccgagca tgtgaccgaa
      901 ctcatgtgac acatcgatgg tatcggcggc accccagtgg ctcatgtttg tcgtcgatgt
      961 gccgacgttg acgacctggt gctcgcccga gttcacgaac tcgatgtcac agacgatgtc
     1021 catgccggag gggcagcagc agtcgcagca gagtttgaag cgatggttcc aggcagcctc
     1081 gatcgcggct tcccacgcgg tgcgctgggc cggggtgatc gtgccgacga ggcggacccg
     1141 gacgcgcgcc gtgacgcggc agttcggctc atcggcgatc agcaggaaac gggcgtgcca
     1201 tccgtaggtg ccgttccagg cctcggagcc gggcggggcg ttcgagatgg tctccgagcg
     1261 cgcctggtca atctcctgct ggaggacgcg atcgcagcgg cgcaggaaca cccagcggag
     1321 gagcaggcac cagacccagc agcgcagccg tacgacgtag agccacaccg ggccaagcag
     1381 ccgcgggagt gggaggaggg gtttctcctc gggaccttcg gggccgggtt tcaggccggg
     1441 gacctcgagg cccgggggca ggtcccgttg tgcgcggacg gtgaacgtct gccaatcgct
     1501 gcgcaggggg tcgtcgcgcg agccgccgag ggcgggaaac tcgccgaagt agcgcactcg
     1561 atattcgccg ggcggcaagc cgcggtcgag gaatccgacg taggtgaggg agagactccc
     1621 accggggacg atgggctcac ccggagcgag gtcggctgcg tccggcgcgg agggcggggg
     1681 cagcaggacc gggcggtcgc tcgcgtcgcg gacatcgagg acgagcgcgg gatgttcggc
     1741 ctgatgcgta ttgagggaag cgggagtcga gctccggttg gtgaagacca ccgtcagcgt
     1801 gaactcgccg ggtacgacct cctctggctt ctcgacacgg atcgcggctt ccagctgcgg
     1861 cattgtgacc tccctgcccg ctttcgcctc gaaacgctgc cccttcgacg taccccgtag
     1921 gcggtgagga tacgcttttg catggccggc ggtgcggggg atgcgcgcga aggagccggc
     1981 ggaatgccgg ctccttcgga tgtggtgcgg gaagcttcag cgtcactagg cgccgccgtc
     2041 gcccgcctcg gcgaggaccg ccggagtgcg tgtcgactgc gccgccggcc gccgcccctc
     2101 gatctcgagg ttggggagcc aggcgagcca gccggggaag taccagttcc agtcaccgag
     2161 cagctccatc gtggccggga cgagcaccga gcggacgatg gtggcatcga cgatgacagc
     2221 gacggcgagt ccgaagccca tctgctggaa catcgccagg tcgccgaggg cgaagccgcc
     2281 gaagacggag accatgatca gcgcggcacc ggtgatgatg gagcccgtcg agcggaggcc
     2341 gtgctcaact gcgtggcggt tgtcgccggt ctcgtcgtag cgctccttga tccggctgag
     2401 caggaagacg tggtagtcca tcgagaggcc gaacaggatg gagaagagga acaggggcag
     2461 ccacgactcg atgacgggca tctggtggaa tccgaggcgc tcggcgccga tgcccttctg
     2521 gaagaccagg acgatcatgc cgtaggcggc accgacggag agcaggttca tcgcgacggc
     2581 cttcagcggg accacgatcg agcggaagac catcgtgagg agcaggaagc tgaagccgag
     2641 cacgacgccg aagacgatcg gggtgtagtg gcggatctcg gcgaccgagt ccacctcggt
     2701 ggcggtggcg ccgccgacga ggacggtcgc gcctgaacca ctgaacgcct gcgggatgta
     2761 gtcgtcgcgg acgcggtcga gggcgtcgcg agcctgggcg ctcgccgggt cgtcgagcac
     2821 ggggaggctc aggagcgcga ggtcaccggc ggcatccgtc ctgagttgcg gcgcgccgta
     2881 gaacgagtca gcggccgcga gcccctcgaa gcgctcgatg gcggactgca ccgcggggtc
     2941 gtggacatcg cgggcctgga tgacgaggtc agccgtgatg acggcggcgc tgtcgaagcg
     3001 gctggtcagc acctcgaagg cgtggcggcc gttgctggtc tcgggcaggg agctgatgcc
     3061 gttgctcccg agctgcatcc agcccaccgc ggaggcggcg ccgagcaaca gaccgccggc
     3121 gacaatgacg ctgaacaccg ggcgggcggt gacgaggcgg gtcgtgaagc cccaaacgcc
     3181 gccttcgttc tcgggggagc gccgacggcc aatcaccggc agggtcagcc agttcacgcg
     3241 gtcaccgagc agccgcagga cggccggcag cagcgtgagg gcggcgagga ccgcggcaat
     3301 gacaacgagg atggcaccga tgccgaaggc acggaaggtg gcgtccggca tgatgagcag
     3361 gccgctcagg gcgatcacca ccgcgatgcc cgagacgaag acggcacgag tcgcggtggc
     3421 cgcggcagtg cgaatcgcct cgtcgcgggc cagaccgagc gagcgctcct cgcggaagcg
     3481 ctggacgatg aagagcgagt agtcgatgcc gacagcgagg ccgatcatcg tgatgatgtt
     3541 ggtcacgaag gtggagaggt cgagcgcccg gccaaccagg gcggtcgtgc cgagggcgag
     3601 gacgatggag gcgagcgcca gcaggacggg gagtccggcg gcgaccgccg cgccgaagac
     3661 gaccagcaga atcaccaggc cgatcccgag gccaaccatc tcaccgcgtt cgagggtctg
     3721 cttcgcgagg tcctcgaact ccgcgttcac gctgccctca ccgaccgtgg tgatgcggta
     3781 gccgtcggcg gcggtgtcgg cggaggcgac ggtggccacg acgggctcgg ccagcgtctt
     3841 cgcctcgttg gagtctccgt tgatgtcgac gatgagcatg gccacctggc cgtcgtcggt
     3901 gatgaagcct gcctcaccat cgaggtaggt gtggaccgcg cggacgttcg cggtgccgcg
     3961 cagctgttcc gccacgctcg ccacggtctg ccgcatcgaa tcggcgctcg cgtggccctg
     4021 tgcctcgacg aggacgtact cgtgcgcgcc cggggcgttc gggtcggcga gcgggccgtc
     4081 gatgaggcgc tgcgcgcgag ccgcctcgga cgagccagac acattgttgt tgtcgctgag
     4141 gacgtcgccc aggctgccgg cggagacaaa cgccgcgccc agcaacatga cccacagcag
     4201 gacggtgagc cagggatggc ggaccgctcc gccggcgacc gc
//
LOCUS       DAORPG010000099         4227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1579_length_4227_cov_2.915868, whole genome shotgun sequence.
ACCESSION   DAORPG010000099 DAORPG010000000
VERSION     DAORPG010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1579_length_4227_cov_2.915868"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PLX85_03300"
     CDS             complement(<1..225)
                     /locus_tag="PLX85_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62231.1"
                     /translation="MTRLLLAALALLASLLQVGALPALFFNASAAPLLPVALLAAWGA
                     LRGPEEIWPALLVAPLPLALLSEQRLGWFLL"
     gene            complement(222..1055)
                     /gene="mreC"
                     /locus_tag="PLX85_03305"
     CDS             complement(222..1055)
                     /gene="mreC"
                     /locus_tag="PLX85_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRC62232.1"
                     /translation="MVRTSNAARLTAFFLLGLVLLALSPLSVSDRIEERGGALLAPAT
                     SVLHRAVRPLSDVLLHAGQIEELSQENADLQQQVARLEAEAAALREHQGAVEAATALR
                     AAVGDEMQQVTASVVVRDPAPGREVLVVDRGRSDGVNVGQPVLGPGATLVGSVVEVQD
                     HRARVRLLSDSSSTVAAVIQQSRVGAALAGSDDRALHLEFVSIDAQVAPGDLIVSSPL
                     GGLLPAGLLIGRVTSRQARPQDLFATIEVEPLTDYARLEHVLIMTGFVPAPGTADEAK
                     P"
     gene            complement(1170..2234)
                     /locus_tag="PLX85_03310"
     CDS             complement(1170..2234)
                     /locus_tag="PLX85_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRC62233.1"
                     /translation="MAINPLASFLGMFSHDIGIDLGTANTLVTVRGRGILIDEPSVVA
                     IDRVSKRVLAIGEEAKRMVGRTPSSIVAVRPLRDGVISDFAVTERMLQHFIRSVHERF
                     GLGIPKPRVVVGIPSGATEVEKRAVHDAALAAGARSAYLIEEPMAAAIGAGLPVMDAA
                     GSMIVDIGGGTTEVAVIALGGIVHSTSIRAAGDAMDQALITYARQMHNLLIGERSAEQ
                     IKIEAGSAYPLDDEGTVDLRGRDLATGLPRAVELSTVEVRDALAPVTNEIIRAVRATI
                     ETTPPELIADLMLHGIAMAGGGSLLRGLDRRIATETRFPVYVAEDPLRAVVRGCGEAL
                     EEAETLQKVQAAVVTRKPPR"
     gene            complement(2369..3493)
                     /locus_tag="PLX85_03315"
     CDS             complement(2369..3493)
                     /locus_tag="PLX85_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HRC62234.1"
                     /translation="MATESEIKRFQGYLQDEINGVYYYRTLSALEQDEQLSGIYRKMA
                     QTEERHLQLWRDELAKAGVPPATETPSRSARLLMWFARRLGPDVVLPVIKAKETGADV
                     VYSAEPRAAEELLPADERQHARIFVAISGRSGLPGSSIGRVESRHRALGGGNALRASV
                     LGANDGLTSNLALVMGVAGASPGHATVVLAGVAGLLAGAFSMALGEWISVTSSREAAE
                     AQIAAEREELERMPEAERDELALIYQAKGLPEAQANELAAHIMADRESALGVLAREEL
                     GIVPEELGSPWTAAMASFVLFAIGALLPVLPFFFTSGVAGIVISAALAGMGLFILGAA
                     ITLLTGRSPWFSGMRQLVLGLVIATITFAIGSAIGGATGI"
     gene            complement(3496..>4227)
                     /locus_tag="PLX85_03320"
     CDS             complement(3496..>4227)
                     /locus_tag="PLX85_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRC62235.1"
                     /translation="PSRLARSIEHWASRGAMDIEGLGEQLAARLVRLELVRSLADIYE
                     LPARREELLQLGGIGPKIVENLFARIEESKTRPLRRLLVALGIRHVGGETATALAVHF
                     GSMDALRAASLEDLSAIDGIGPIVAASVYDYLHDEEYAALLDRLQTLGLRMTDETAAR
                     GGPLENETIVVTGSLERWSRDRIEALIKQLGGKVGSSVTKKTTMLVAGEGGGSKRDKA
                     EATGTPILDEEQFLAVLRERGWSGD"
BASE COUNT          647 a         1357 c         1646 g          577 t
ORIGIN      
        1 gagcaggaac cagccaagcc gctgctcgga caggagcgcg agcggcagtg gcgcgacgag
       61 gagcgcgggc cagatctcct cggggcctcg cagcgcgccc caggccgcca ggagcgcgac
      121 cgggagcagc ggagccgcgg acgcgttgaa gaagagcgcc ggcagcgcgc ccacctgcag
      181 caacgaagcg agcagggcga gcgcggccag caggaggcgg gtcacggctt cgcctcgtcg
      241 gctgtgccgg gagcggggac gaagccggtc atgatcagga cgtgctcaag acgggcgtag
      301 tcggtcagcg gctcgacctc gatcgtggcg aacaggtcct gcgggcgtgc ctggcgggag
      361 gtgacgcggc cgatcaggag gcccgccggc agcagtccgc cgagcggcga actgacgatg
      421 aggtcgccgg gagcgacctg cgcgtcgatc gagacgaact cgaggtggag cgcgcggtca
      481 tcggaacccg cgagggccgc gccgacccgt gactgctgga tcacagccgc caccgtggac
      541 gaggagtcgg agagcagccg cacccgcgcg cgatgatcct gcacttcgac cacgctcccg
      601 acgagggtcg cgcccggccc caggaccggc tgaccgacgt tcacgccgtc ggagcggccg
      661 cgatcgacca cgagcacctc gcggcccggc gctggatcgc gcacgacgac cgaggccgtt
      721 acctgctgca tctcatcgcc gaccgctgcc cggagtgcgg tcgcggcctc gacagcgccc
      781 tggtgttcgc gcagcgcggc tgcctcggcc tcgagtcgtg cgacctgttg ctgcaggtct
      841 gcgttctcct ggctgagttc ctcgatctgg ccagcgtgga gcagcacgtc cgacagcggg
      901 cgcaccgcgc ggtgcagcac cgacgttgcg ggcgcgagca gcgcgccgcc gcgttcctcg
      961 atgcggtcgg aaaccgagag cggcgagagg gcgagcagca ccagcccgag gaggaagaag
     1021 gcggtcaggc gcgcggcgtt gctggtgcgg accaacggag gttccacctc gaagggcgtg
     1081 acgcaacggc gcgtcctgcg ggggagcgaa ttggagagag ccgtgtgggg agtcgcagcc
     1141 gagccggtgg ggataccggc agggctatgt tagcgcggcg gcttgcgggt tactaccgcg
     1201 gcctgaacct tctgcagcgt ttctgcctcc tcgagcgcct ccccgcagcc tcggacgacg
     1261 gcgcgcagcg ggtcttcggc cacatacacc gggaagcgcg tctcagtggc gatgcggcgg
     1321 tcgaggccgc ggaggagcga gccaccgccg gccatggcga tgccatgcag catgaggtcg
     1381 gcgatcagct cgggaggcgt cgtctcgatg gtggcgcgca cggcgcggat gatctcgttg
     1441 gtgaccggcg cgagggcgtc gcgcacctcg acagtggaga gctcgacggc acgcggcagg
     1501 ccggtggcga ggtcgcggcc ccggaggtca acggtgccct cgtcgtcgag cgggtaggcc
     1561 gagcccgcct cgatcttgat ttgctcggcg ctgcgctcgc cgatgagcag gttgtgcatc
     1621 tgtcgcgcgt aggtgatgag cgcctggtcc atagcgtcgc ccgccgcgcg gatcgacgtg
     1681 gagtgcacga tgccgccgag ggcgatgacg gcgacctcgg tcgtgccacc cccgatatcg
     1741 acgatcatcg agccggccgc gtccatgacg ggcaggcccg cgccgatggc ggcggccatg
     1801 ggctcctcga tgaggtaggc ggagcgggcg ccggcggcga gggcggcgtc gtggacggcg
     1861 cgcttctcga cctcagtggc cccggagggg atgccgacga cgacgcgcgg cttgggaatg
     1921 ccgaggccga agcgctcgtg tacggagcgg atgaagtgct ggagcatgcg ctcggtgacg
     1981 gcgaagtcgg agatcacgcc gtcgcgcagt gggcggacgg cgacgatgct cgagggcgtg
     2041 cggccgacca tgcgcttcgc ctcctcgccg atggcgagga cacgcttcga gacgcgatcg
     2101 atggcgacca cggagggctc gtcaatcagg atcccgcgac cgcggaccgt gacgagcgtg
     2161 ttcgcggtgc cgaggtcgat gccgatgtcg tgcgagaaca tcccgaggaa ggacgcaagg
     2221 ggattgatcg ccacagcagt gccgttctgg ggaggtcggc ccggggatgg ggggagtgcc
     2281 cggagcgccg cgcaccggag gcagggcgag tatacggagg gcgggggcgg cgggacaagg
     2341 cgaggagcct cggacgccgc gaggctgcct agatcccggt cgcgccgccg atggccgagc
     2401 cgatggcgaa cgtgatcgtc gcgatcacga ggccgaggac gagctggcgc atgccggaga
     2461 accaggggct gcggccggtg agcagcgtga tcgcggcgcc gaggatgaag aggcccatgc
     2521 cggcgagcgc tgcgctgatg acgatgccag cgacgccgct ggtgaagaag aacggcagca
     2581 cggggagcag cgcaccgatg gcgaacagca cgaacgaggc catggcggct gtccaggggg
     2641 agcccagctc ctcagggacg atgcccaact cctcgcgggc gaggacgccg agggcggact
     2701 cgcggtcggc catgatgtga gcggccagtt cgttcgcctg cgcctcgggg aggcccttgg
     2761 cctggtaaat aagcgcgagc tcgtcgcgct cggcctcagg catgcgctcg agctcctcgc
     2821 gctcggcggc gatctgcgcc tcggcggctt cgcgcgagga cgtcacggag atccactcgc
     2881 cgagggccat cgagaaggcg ccggcgagga ggccggcgac gccggcgagc accactgtcg
     2941 cgtggccggg gctggcgccg gcgacgccca tcaccagggc caggttcgag gtcagcccgt
     3001 cgttcgcgcc gagcacggag gcgcggagcg cattgccccc gccgagggcg cggtgtcgcg
     3061 actcgacccg gccaatggag gagccgggga ggcccgagcg cccggagatg gcgacgaaga
     3121 tgcgggcgtg ttgccgctcg tcggccggga gcaactcctc ggcggcgcgt ggctcggcag
     3181 agtagacgac gtcggcgccg gtctcttttg ccttgatgac gggcaagacg acgtcggggc
     3241 cgaggcggcg cgcgaaccac atcaggaggc gcgcgctcct cgatggcgtc tcggtggcgg
     3301 gcgggacgcc cgccttcgcc agctcgtcac gccagagctg gaggtggcgc tcctcggtct
     3361 gggccatctt gcggtagatg ccgctgagct gctcgtcctg ctcgagcgcc gaaagggtcc
     3421 ggtagtagta gacgccgttg atctcgtcct ggaggtagcc ctggaagcgc ttgatttcgg
     3481 actcggttgc caccgtcagt ctccgctcca gccgcgctcg cgcagcaccg cgaggaactg
     3541 ctcctcgtcg aggatggggg tgccggtggc ctcggccttg tcgcgcttgg agccgccacc
     3601 ctcgcccgcc accagcatcg tcgtcttctt cgtcacgctc gacccgactt taccgcccag
     3661 ctgcttgatg agggcctcga tgcggtcgcg ggaccagcgt tcgagtgagc cggtgacgac
     3721 gatcgtctca ttttcgagcg gaccgccacg ggcggctgtc tcgtccgtca tgcggaggcc
     3781 aagcgtctgc agacggtcga ggagcgcggc gtattcctcg tcgtggaggt agtcgtagac
     3841 ggaggcggcg acgatcgggc cgatgccatc gatggcgctc agatcctcga gcgaggcggc
     3901 gcgcagggcg tccatgctgc cgaaatgtac ggcgagggcg gtggcggtct cgccgccgac
     3961 gtggcggatg ccgagagcga cgaggaggcg gcgcaagggg cgcgtcttgc tctcctcgat
     4021 gcgggcgaac aggttctcga cgatctttgg gccaatgccg ccgagttgca gcagctcttc
     4081 ccggcgggcg ggcagctcgt agatgtcggc gaggctgcgg accagctcga ggcggaccag
     4141 gcgggcggcg agctgctcgc cgaggccctc gatatccatt gcgcctcgcg aggcccagtg
     4201 ctcgatcgag cgtgcgaggc gggaggg
//
LOCUS       DAORPG010000100         4149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1647_length_4149_cov_2.622863, whole genome shotgun sequence.
ACCESSION   DAORPG010000100 DAORPG010000000
VERSION     DAORPG010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1647_length_4149_cov_2.622863"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            405..1229
                     /locus_tag="PLX85_03325"
     CDS             405..1229
                     /locus_tag="PLX85_03325"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRC62236.1"
                     /translation="MLPTDVRPVDARSILTPASGFIRAYRYTLNPYSGCRFACEYCYA
                     RAFAPTEEQRRTWGEWASAKRNAARLIARACADGTLRSGDAVYMSSATDPYQPAEQRL
                     RLTRSILEAMIEWGVQPRLTVQTRSPIAARDIDLFRRFERIRVNITVTTDSERVRLRY
                     EPSAPSIRARLRTARALAQAGVPIGISVSPMLPMEDLEGFARAIAALEAAEYVSQYMQ
                     PPTRVFAAGTRPETLRKAREDGWTPHEYERARAVLQRVLGPRRPLLEGDEGFAPVP"
     gene            complement(1235..2434)
                     /locus_tag="PLX85_03330"
     CDS             complement(1235..2434)
                     /locus_tag="PLX85_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRC62237.1"
                     /translation="MESDVPRLLAKFESGALRRPTAARPNVVDLAGAVWSAAGVPGLD
                     LTPAARTIEAQLGGREHLVFVLADGLGMQLLESMPSATYLWTHLDGTLDTVFPSTTAV
                     ALTTFATAAWPAAHGVVGHYVHLDGVGVVTPLLGATRSDGRPVPEANGDGRLFLVPSL
                     ASRVPRPALSVLPAAVVNSPFTAYATAAPRVGYRSLNEAADLAIDFIQAAREPTFTYL
                     YVSRIDETVHELGPLRLEAVGAVGDLAHVMARLAAAVGARARIVLTADHGHLAVRPSS
                     RRVIRGVDDLAGMLAGGPTGDARTVFFHLQPGALEAFADRFRERFGVAYCLLTPDEVE
                     DLHLLGPAPLAAETKRRLGDAVAISFGDDTLEVRPKGAPADRRLAWPSHHSGLTAAEV
                     RVPLVIV"
     gene            2503..>4149
                     /locus_tag="PLX85_03335"
     CDS             2503..>4149
                     /locus_tag="PLX85_03335"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62238.1"
                     /translation="MQTSTQGTDGAAAGINPGVLREQLGAAYERYSDLEAERHAALLS
                     RLGGGAALAMAVDALADGRWSLAICCADAVGTLSVIAGLCTAYGLDIEAGDVFTVDTP
                     SSSEAAVHVDPRTGRLRRVAEASAGPRRRILDVFEVRCGATDRELWRRFEDDLGGLIM
                     LLASGQGEQARDLVIDRVSEVLRATRSEDAPLAAIQVEVDNGASPTSTVLRIRSLDAP
                     GFLFEFTNALALLRVNIERAEVRTVAGETQDTFWVTEAAGGKLPAGGRSDQLRVAAAL
                     IKQFTLLLPRSPNPAQALRQFSALTRQLLARPDWTSEVRDLESGEVLRTLAELMGVSE
                     FLWEDFLRIQHENLFPVLGDMPGLDAARPAPELRRLLRERLDGGGDLEGRRRALNAFK
                     DREMFRIDLRHISGRTDFSGFADELSALAEVVVEAAVGLAEESVAERYGRPRNTDGQP
                     CGWVTCALGKFGGRELGFASDIELLFVYEGEGSTDGREPVANAEYFDACVQELLRTIV
                     TRSEGIFEIDLRLRPHGNAGSLATSLEGFRRYYSADGEAQQ"
BASE COUNT          652 a         1243 c         1647 g          607 t
ORIGIN      
        1 ggcgccagac gcgggtgacc cgtgggaggg ggacgtccat cttcatgaac gcgggaagcg
       61 tgtcagagtt cgaccgcaca gtcgtgaagc cggcttcctg cagttcttga acgtaggccc
      121 tgcaggtatg ccaccgaggc atcggccgtg agtcgatcga ttcgttcgag agtggtgggc
      181 cacgatgagt gttgcaaggc cacatggccc gcaatcgggt gactctcatt tgtcggtgga
      241 aaaccacggt ggagcgtcat gagatgacgt tgccctcgta ggtactttcc gcgaagtcgg
      301 acgtgacacc gggctgctct cgcgtgcaat gacattccgc cgttggagtt tggagggcag
      361 aacacgacat tcgttgtgtg cggagcgtgc aaccatcacc cgacatgctt cctaccgacg
      421 tgcggcctgt agatgcgcgg tcgatcctga cgccggcgtc ggggttcatt cgggcctacc
      481 ggtacacgct taatccgtat tcggggtgtc ggttcgcgtg tgagtactgc tatgcacggg
      541 cgttcgcgcc caccgaggag caacggcgca cgtggggcga gtgggcctcg gcgaagcgga
      601 acgcggcgcg gctgattgcg cgtgcgtgcg cggacgggac gctgcggtcg ggtgacgcgg
      661 tgtacatgtc gagcgcgacc gacccatacc agccggcgga gcagcggctg cggctgacgc
      721 gttcgatcct cgaggcgatg atcgagtggg gggtgcagcc gcggttgacg gtgcagactc
      781 gcagcccgat cgcggcgcgg gacatcgacc tgttccgacg gttcgagcgg atccgcgtga
      841 acatcacggt gacgacggac tcggagcggg tgcggctgcg gtacgagcca agcgcgccct
      901 cgattcgggc gcggctgcgg acggcgcgag cgcttgcgca ggcgggggtg ccgatcggga
      961 tctcggtgag cccgatgctg ccaatggagg acctcgaagg gttcgcgcgc gcgatcgcag
     1021 cactggaggc ggcggagtat gtgtcgcagt acatgcagcc gccgacccgg gtgttcgcgg
     1081 ccgggacgcg gccggagacg ctgcggaagg cgcgtgagga cggctggacg ccccacgagt
     1141 acgagcgggc gcgcgcggtc ctccagcggg tgctggggcc tcgacggccg ttgctcgagg
     1201 gtgacgaggg gttcgcgccc gtgccgtaag gtcgttacac gatcacgaga gggacacgca
     1261 cctcggcggc cgtgaggccg gagtggtgcg agggccaggc gaggcgacgg tcggcaggcg
     1321 cgccttttgg gcgcacttcg agcgtgtcat cgccgaagga gatggcaacg gcgtcgccga
     1381 ggcggcgttt cgtctcggcg gcgaggggag ccgggccgag caggtgaagg tcctcgacct
     1441 cgtcgggagt cagcaggcaa taggcaacgc cgaagcgctc gcgaaagcgg tccgcgaacg
     1501 cctcgagggc gccgggctgc aggtggaaga agacggtgcg ggcgtcgccg gtggggccac
     1561 ccgcgagcat gccggcgagg tcgtccacgc ctcgaatcac acggcgcgag gacgggcgca
     1621 cggcaaggtg gccgtggtcg gcggtcagca cgatgcgtgc gcgcgcacca acggcggccg
     1681 cgaggcgggc catgacgtgc gcgagatcgc cgaccgcgcc gaccgcctcg aggcgcaggg
     1741 ggccgagctc atggacggtc tcatcgatgc gggagacgta caggtacgtg aacgtgggtt
     1801 cgcgggccgc ctggatgaag tcgatcgcga ggtcggcggc ctcgttcaga ctgcgatagc
     1861 cgacgcgcgg cgctgcggtg gcgtaggcgg tgaacgggct gttcacgacg gccgcgggaa
     1921 gcacagagag cgccgggcgg ggcaccctcg aggcgaggga ggggacgagg aagaggcggc
     1981 cgtcgccgtt cgcctcgggg acgggacggc cgtcggagcg agtcgcgccg agcaacgggg
     2041 taacgacgcc gacgccatcg aggtggacgt agtggccgac gacgccgtgg gcggcgggcc
     2101 aggcggccgt ggcgaacgtg gtcagcgcga cggcggtggt ggaggggaat acggtgtcga
     2161 gcgtgccgtc gagatgggtc cagaggtagg tggcggacgg catcgactcg aggagctgca
     2221 taccgaggcc gtcggcgagg acgaacacga ggtgttcgcg gccaccgagt tgcgcctcga
     2281 tggtacgggc agcgggagtg aggtcgaggc cggggacgcc ggcggcggac cacacagcgc
     2341 cggccaggtc gacgacgtta ggacgcgcgg cggtgggtcg gcgcagcgcg ccgctttcga
     2401 acttcgcgag caggcgagga acgtcgcttt ccacacgtga gtgtgtgccg acggaggcgt
     2461 gctggtgcaa ccgtgcgtgt gcccagcgct acgatagcgt cgatgcagac ctcgacgcag
     2521 gggactgatg gagccgctgc gggtatcaac ccgggggtgc ttcgcgagca gctcggggcg
     2581 gcgtacgagc ggtacagcga cctcgaggcg gagcgacatg cagccctgct gagtcgcctc
     2641 ggtggcggtg cggcgctggc gatggcggtg gacgcgctgg cggatggccg ctggtcgctc
     2701 gccatctgct gcgcggatgc agtgggcaca ctctcggtga tcgcgggact gtgcacggcg
     2761 tacggcctcg atatcgaggc gggagacgta ttcaccgtcg acaccccgag cagctcggag
     2821 gcggctgtgc atgtcgaccc gcggaccggg cggctgcgac gagtggccga ggcgagcgcc
     2881 ggaccgcgcc gccgcatcct cgacgtgttc gaggtccgct gcggggccac tgaccgtgag
     2941 ctgtggcggc gattcgagga cgacctcggc ggcttgatca tgctgctcgc ctcgggccag
     3001 ggggagcaag cgcgcgacct cgtgatcgac cgcgtgagcg aggtgttgcg ggcgacgcgg
     3061 tcggaggacg cgcccctcgc ggccatccaa gtcgaggtgg acaacggggc gtcgccaacc
     3121 tcgacggtgc tgcggatccg gtcgctcgac gcgccgggat tcctgttcga gttcacgaac
     3181 gcgctcgccc tgctgcgcgt caacatcgag cgggccgagg tgcggacggt cgcgggcgag
     3241 acgcaggaca cgttctgggt gaccgaggca gccggcggca agctgccggc agggggccgc
     3301 tccgaccagt tgcgcgtggc ggcggcactg atcaagcagt tcacgttgct gctgcctcgg
     3361 tcgccgaacc cggcccaggc gttgcgccag ttcagcgcgc tcacgcggca gctcctcgcg
     3421 cggccggact ggacctcgga agtgcgcgac ctcgaatcgg gcgaggtgtt gcggacgctc
     3481 gccgagctga tgggcgtgtc ggagttcctg tgggaggact tcctgcgcat ccagcacgag
     3541 aacctcttcc cggtgctggg cgacatgccg ggactcgatg ccgcacggcc agccccggaa
     3601 ctgcgccggc ttttgcgaga gcgcctcgat ggcgggggcg acctggaggg gcggcgacga
     3661 gcactgaacg cattcaagga ccgcgagatg ttccgcatcg acctgcggca catcagcggg
     3721 cggacagact tcagcggctt cgctgacgag ctgtccgcgc tcgccgaggt cgtcgtcgag
     3781 gccgcggtcg ggctcgcgga ggagtccgtt gcggagcggt atggccgacc tcgaaacaca
     3841 gacgggcagc catgcggatg ggttacatgc gcgctcggga agttcggggg acgggagctc
     3901 ggattcgcgt ccgacatcga gctgctgttc gtgtacgagg gcgaggggag cacggacggg
     3961 cgcgagccgg tcgcgaacgc cgagtacttc gatgcctgcg tgcaggagtt gctgcgcacc
     4021 atcgtgaccc ggagcgaggg gatcttcgaa atcgacttgc ggctgcggcc gcacggcaac
     4081 gcgggatcgc tcgcgacgag cctcgagggg ttccgccgct actactcggc ggacggcgag
     4141 gcacagcag
//
LOCUS       DAORPG010000101         4136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1655_length_4136_cov_3.297476, whole genome shotgun sequence.
ACCESSION   DAORPG010000101 DAORPG010000000
VERSION     DAORPG010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1655_length_4136_cov_3.297476"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2098
                     /locus_tag="PLX85_03340"
     CDS             <1..2098
                     /locus_tag="PLX85_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HRC62239.1"
                     /translation="DGVRTPMQWTADRNAGFSTADSARLYLPVISDPQYGYQAVSVEA
                     QERSPSSLLNWMRRMIALRRQRRRVFGSGSLHFLHPENRHILAYLRHHEGEVVLVVAN
                     LSRFVQPVSLDLGAYAGCQPIEVIGGGVFPVITEQPYFLSLGPHGFYWFDLVPQSEPI
                     VITSEPSGGVEVTVDGDWDSLFRGEALRALEEEVLPNALPPRRWFGGKDRAIASVRVA
                     DAIPLTAGAAPSWLALADVTFEGGAPEAETYALPLAVAAGQAARRVRDESAASVLCSV
                     SGPRGRGLLYDGLAAPEVVRHLLGLLRDGRPIRGRQGQLRAEPARAFEELFDALDTRP
                     TVQSMSGEQSNSSVRIGEQVVLKLLRRVTPGPHPEVEAGRHFERVGFERSPRLAGTFE
                     YTPRNGTPSTVAVAHELVWNQGDGWSYTTAELARFLGGVDSPPETSDEPLDPNYVESA
                     RVLGQRTAEMHLALADTRGLETFRPEPFEADDVETVAARIARRLRGLAPALARLSGLA
                     PADAEAAAALRAAGLRRGGWRYHLAPAEGGLGAKIRCHGDYHLGQVLWALNDFHIVDF
                     EGEVGLSLDERRAKASPLTDVAGMLRSFDYAATFSLRERTREMPTTDALVAALEPTAA
                     RWVREASAAFLDTYRSVVEGAGLFPPEPDQFEALLRLHLLDKALHELEYELANRPEWV
                     TIPLRAAAALIEARAG"
     gene            2095..4026
                     /gene="glgB"
                     /locus_tag="PLX85_03345"
     CDS             2095..4026
                     /gene="glgB"
                     /locus_tag="PLX85_03345"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HRC62240.1"
                     /translation="MTVPPSFPAEWLTTYDLHLLAEGTHLEAFEKLGAHPFTVDGATG
                     TAFAVWAPNARGVSVIGDFNGWHNDAHPLRLRPEAGVWEGFVAGVYPGDLYKFAVLQS
                     DGSTVQKADPFAFYSEQRPRSASIVWDLDRYAWGDSQWIEKRPAAQAREAPIAVYEVH
                     LGSWARVPEEGDRWLTYRELAPRLAEYVRDLGYTHVELLPITEHPFDASWGYQPLGYF
                     APTSRFGTPDDFMAFVDTLHQAGIGVLLDWVPGHFPTDAHGLARFDGTYLYEHADPLL
                     RDHPDWGTSTFNLGRREVANFLIASALFWIRRYHVDGLRVDAVASMLYRDYSRREGEW
                     IPHPEGGRNDLDAVRFLQRFNETVYTQQPGVITVAEESTAWPLVSRPVSAGGLGFGFK
                     WNMGWMHDTLEFMARDPVHRGHHLDNLTFGLLYAFNEDFVLVYSHDEVVHGKGSMLAK
                     MPGDDWQKFANLRLLYGYQYGHPGKKLLFMGSEFGQWSEWNEAGSLEWDLLQYERHRG
                     LQQWVRALNELYRTDPALYELDGEGEGFSWIDCTDSTHAVVSFLRKSKGGARAVLVVC
                     NFTPVPREGYRIGSPLPGDWEVLLNSDETRWGGSGYSVPPRFQAGAHPMHGYPQSLDL
                     TLPPLGLLVLQPVAQGDRG"
BASE COUNT          601 a         1498 c         1374 g          663 t
ORIGIN      
        1 cgacggagtc cgcacgccca tgcagtggac cgccgaccgc aacgccggct tctctaccgc
       61 ggattccgcc cggctctacc tgcccgtgat ctcggacccg cagtacggct accaggctgt
      121 gagcgtcgag gcgcaggagc gcagcccctc gtcgctgctc aactggatgc gtcggatgat
      181 cgcgctgcgg cgccagcgcc gtcgcgtctt cggttccggc tcgctgcact tcctgcaccc
      241 cgagaaccgc cacatcctcg cctacctccg ccaccacgag ggcgaagtcg tgctcgtcgt
      301 cgcgaacctg tcgcgcttcg tgcagcccgt ctccctggac ctcggtgcct atgccgggtg
      361 ccagccgatc gaggtgatcg gtggcggcgt gttcccggtg atcacggagc agccgtattt
      421 cctgtcgctc ggcccgcacg gcttctactg gttcgacctc gtcccccagt cagagcccat
      481 cgtgatcaca tccgagccct cgggcggcgt cgaggtcacc gtagacggcg actgggactc
      541 gctcttccga ggcgaggctc tgcgggccct cgaggaggag gtgctcccga acgccctccc
      601 gccgcggcgc tggtttggcg gcaaggaccg cgctatcgcg tccgttcgcg tcgcggacgc
      661 catcccgctc acggccggcg ccgccccctc ctggctggcg cttgcggacg tgaccttcga
      721 gggcggcgcg cccgaagccg agacctacgc gctgccgctc gcggtggctg ccgggcaagc
      781 cgcacgccgg gtgcgcgacg agtcggcggc gagcgtgctg tgctccgttt ctggcccacg
      841 cggccgaggc ctcctgtatg acggcctcgc ggcgcccgag gtcgtgcgtc acctgcttgg
      901 cctgctccgc gacggccgcc ctatacgagg ccgtcagggc cagctgcgcg cggagccggc
      961 ccgggcgttc gaggagctct tcgacgcgct cgacacgcgc cccaccgtgc agtcgatgtc
     1021 gggcgagcag agcaacagtt cggtgcgcat cggcgagcag gtcgtcctga agctgttgcg
     1081 ccgcgtgacg cctggtccgc accccgaggt cgaagctggc cggcacttcg agcgcgtagg
     1141 gttcgagcgg agccctcggc tggccggaac cttcgaatac accccgcgta acgggacacc
     1201 atcgaccgtc gccgtcgcgc atgagctcgt gtggaaccag ggcgatggtt ggagctacac
     1261 cacggccgaa ctggcgcgct tcctcggcgg cgtcgactcc ccgcccgaga catcggacga
     1321 gcccctggac ccaaactacg tcgagtccgc ccgcgtcctc ggccaacgca cggcggagat
     1381 gcacctcgcg ctcgccgaca cgcgcggcct cgaaacgttt cggcccgagc cgttcgaggc
     1441 ggacgacgtg gagacggtgg cggcgcgtat cgcgcgacgc ctgcgtgggc tcgcaccagc
     1501 gctggcccgg ctgtcgggcc tggctcccgc ggatgcggag gccgccgcgg cgctgcgagc
     1561 cgccgggcta cgtcgcggtg gctggcgata ccatctggcc ccggccgaag ggggactcgg
     1621 cgcgaagatc cgttgccacg gcgactacca cctcggccag gtgctgtggg cgctgaacga
     1681 cttccacatc gtcgacttcg agggcgaggt ggggctgtcg ttggacgagc gacgcgccaa
     1741 ggcctcgccc ctgacggacg tcgccggcat gctccgctcc ttcgactacg ccgcgacgtt
     1801 ctcgctgcgc gagcggacgc gcgagatgcc gacgaccgac gccctcgtgg cggcgctcga
     1861 acccaccgcc gcccgctggg tgcgcgaagc gagcgcggcc ttcctcgaca cctaccgtag
     1921 tgtggtcgaa ggagcaggac tcttcccgcc ggagccggac cagttcgagg cgttgctgcg
     1981 gctgcacctg ctggacaaag cgctgcacga actggagtac gagctggcga accgccccga
     2041 atgggtcacc atcccgctgc gtgccgcggc cgcgttgatc gaggcgcggg ccggatgacc
     2101 gtccccccgt ccttcccggc cgaatggctc accacctacg acctgcatct gctggctgaa
     2161 ggcacccacc tcgaggcgtt cgagaagctc ggggctcatc ccttcacggt ggacggtgcg
     2221 accggcacgg cgtttgccgt ctgggcgccg aatgcgcgcg gcgtctcggt catcggcgac
     2281 ttcaacggct ggcacaacga tgcccatccg ctccgcctcc gacccgaggc gggcgtgtgg
     2341 gagggcttcg tggccggcgt ctaccccggc gacctctata agttcgccgt gctgcagtcc
     2401 gacggctcga ccgtgcagaa ggccgacccg ttcgcgttct actcggagca gcgcccgcgc
     2461 agtgcctcca tcgtgtggga cctggaccgc tacgcctggg gcgacagcca gtggatcgag
     2521 aagcggccgg ccgcccaggc gcgagaagcg cccatcgcgg tctacgaggt gcacctcggt
     2581 tcgtgggcac gcgtccccga ggagggcgac cgctggctca cctaccgcga actcgcgccg
     2641 cgcctcgccg agtacgtccg ggatctgggg tacacccacg tcgagctgct gccgatcacg
     2701 gagcacccct tcgacgcctc gtggggctac cagccgctcg gttacttcgc cccgacgagc
     2761 cgcttcggca cgccggacga cttcatggcc ttcgtcgaca cgttgcacca ggcggggatc
     2821 ggcgtcctcc tcgactgggt gccgggccac ttcccaaccg acgcccatgg cctcgcgcgc
     2881 ttcgatggca cctatctgta cgagcatgcc gacccgctcc tgcgcgacca ccccgactgg
     2941 gggacgtcga ccttcaacct cggacgccgc gaggtggcca acttcctgat cgccagcgcc
     3001 ctcttctgga tacgccgcta ccacgtcgac ggcctgcgcg tcgacgccgt cgcctccatg
     3061 ctctaccgcg actactcgcg gcgcgagggc gagtggatcc cccaccccga gggcggccgc
     3121 aacgacctcg acgccgtgcg cttccttcag cgttttaacg aaacggtgta cacgcagcag
     3181 cctggcgtga tcaccgtggc tgaggagtcc acggcctggc cgctcgtctc acggccagtg
     3241 agcgcgggcg ggctcggctt cggattcaag tggaacatgg ggtggatgca cgacaccctc
     3301 gaattcatgg cgcgcgatcc cgtgcatcgt ggccatcacc tggacaacct cacgttcggc
     3361 ctgctgtacg ccttcaacga ggactttgtg ctcgtctact cccacgacga ggtggtgcac
     3421 ggcaagggct cgatgctcgc gaagatgccg ggcgacgact ggcagaagtt cgcgaacctg
     3481 cggttgctct acggctacca gtacgggcat cccggcaaga agctgctctt catgggctcc
     3541 gagttcgggc aatggtccga atggaacgag gccggcagtc tcgagtggga cctgctgcaa
     3601 tacgagcgcc atcgaggcct gcagcagtgg gtgcgcgccc tgaacgagct gtaccgcacc
     3661 gaccccgccc tctacgagct cgacggcgag ggcgaaggct tctcgtggat cgactgcacc
     3721 gacagcaccc atgccgtcgt gagcttcctc cgcaagtcaa agggcggcgc gagggccgtc
     3781 ctggtcgtgt gcaacttcac ccccgtcccc cgcgagggct accgcatcgg gagcccgctg
     3841 cccggggact gggaggtgct gctgaacagc gacgaaacgc gttggggcgg cagcggctac
     3901 agcgtccccc cgaggtttca ggcaggggcg caccccatgc acggctatcc gcaatccctc
     3961 gatctcacgt tgccgccact cgggctactt gtgttgcagc cggtcgcgca gggcgaccgc
     4021 ggatgaccac gctgagcctg gtggtgcacg gccaccaacc gcccggcaac ttcgattccg
     4081 tctttcgacg tggcgtggcc cagtgctacc ttccgttctt cgagctgctc gaggag
//
LOCUS       DAORPG010000102         4089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1698_length_4089_cov_3.111800, whole genome shotgun sequence.
ACCESSION   DAORPG010000102 DAORPG010000000
VERSION     DAORPG010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1698_length_4089_cov_3.111800"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..1175)
                     /locus_tag="PLX85_03350"
     CDS             complement(42..1175)
                     /locus_tag="PLX85_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC62241.1"
                     /translation="MDTNTLILGAGFGGLELASRLSDAFGPDAGVTLIDQNEGFVFGF
                     SKFELMFGRATLDEVMHRYAELVKPGVAFRRERILSIDPAARRVVTDRETYEAQTLVV
                     ALGAEYDPGATPGFVEGGHEFYSVPGAIALHDVLLGFEGGAVVIGVLGQPFKCPPAPC
                     EAAMLLEEWLTKRGQRERSSVTLVHPWGTPVPPSPDGSVAIRQRFEERGITFLGDRTI
                     THIDPAAKTVHIADGSTVPYDLFLGIPIHRVPAVVVESGLAEDGWIPVDKTNLATRFP
                     GVYALGDVTSAPVPKAGVFAESAARALASYLIDQAKQTADFRPFDGSGSCYVEFGGGL
                     VGRVDADFLTGPQPRGPFVAPSEELAREKAGFATTRDRRWFRA"
     gene            1322..3856
                     /locus_tag="PLX85_03355"
     CDS             1322..3856
                     /locus_tag="PLX85_03355"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRC62242.1"
                     /translation="MLDTMPLEGVRVLDLTRHTVGPFATRLLGDYGADVIKIEAPGGD
                     PARMLPPFAGDEPGLERSGTFLFLNTNKRSVVLDLKSEDGRERFLQLARGADAVVENF
                     RPGTMERLGLGYEVLAGLNPRIVVTSISNFGQDGPYRDWEGTDLTIYAMGGPMISTGD
                     VDHEPLKVAGRWAGYQAGFVAALATAVGLWGARLRGEGEHLDVSILEALTHNIDGRLI
                     SLLQYQYTGRPAVRRPRAAGAGNGVFPCADGWCLLTGGGLANMSAFMRMIGQEQLLES
                     PEWRDPGAFADPDRIEEFNAYVLGWSVMKTKAEVRDACEQFGVMGAPLNTVADMLTDK
                     SLVYRKFFEEIDHPSTGPVQYPGYHFRLHGEHGPEMPPRRRAPLLGEHTDEVLAELEQ
                     ATAAVVAPRAPSSARRDEAGYRPPLEGIRILDLTLVLAGPFATMHLADWGAEVIRIES
                     LQHPQPFTRGMPFVRPTLDMVRRQMVPPYFPDDDPGERAWNKPANFTHHARGKKSMTL
                     DLSRPEGQEVFERLVAQADGVIENNLPPHIEKLGITWERLSKINPRLVLLRQPAFGLD
                     GPYRAYRTFGNHMEAIAAHPVIRAYPDLSLDYAPQGIPADAASGIAGAYAFLMGLHQR
                     ERTGKGVMLELASAENIIPFMGEFVMDYTLNGRSWEHMGNDHFFLAPHNVYRCADGDR
                     WVAIVARNEDEWRRLCEVMRRPELANDPRFASMEARYANRRELDAAITEWTVLRDASW
                     VMQRLQAEGIAAGVVMNDRDVLDDPQHRAREFFVEIEGPDTGRRQYVRRAWRASKTPW
                     REMRHPVLLGEDNEYMYRQLLGFSDEEYRRFEELGHIGTEYPAGIP"
BASE COUNT          663 a         1250 c         1597 g          579 t
ORIGIN      
        1 ggcgccggag ttgaagccgg cgcagctctt gttactcgga cctaggctcg gaaccaacgt
       61 cggtcgcgag tggtggcgaa gccggccttt tcgcgggcga gttcctcgga cggggcgacg
      121 aaggggcctc ggggctgggg gcccgtgagg aagtcggcgt cgacgcgccc gacgaggccg
      181 ccgccgaact cgacgtagca ggagcctgag ccgtcgaagg ggcggaagtc ggcggtctgc
      241 ttcgcctggt cgatgaggta gctggccagg gcgcgggcgg cgctttcggc gaacactccc
      301 gccttgggta cgggcgcgct ggtgacatcg ccgagggcgt agacgccggg gaagcgggtc
      361 gcgaggttcg tcttgtcgac ggggatccag ccgtcctcgg cgaggcccga ctcaacgacg
      421 acggcgggca cgcggtggat gggaatgccc aggaagaggt cgtatggaac cgtgctgccg
      481 tcggcgatgt ggactgtctt cgcggccgga tcgatgtggg tgatggtgcg gtcgccgagg
      541 aacgtgatgc cgcgttcctc gaagcgctgc cggatggcca cggagccgtc cggcgagggc
      601 ggaacagggg tgccccaggg gtggacgagc gtgacgctcg agcgttcgcg ctggcctctc
      661 ttggtgagcc attcctcgag gagcatggcg gcctcgcagg gggcgggcgg gcacttgaag
      721 ggctgcccga ggacgccgat gaccaccgcc ccgccctcga agccgagcag aacgtcgtgg
      781 agggcgatcg cgccggggac ggagtagaac tcgtggccgc cctcgacgaa accgggcgtg
      841 gcgccggggt cgtactcggc accgagggcg acgacgaggg tttgtgcctc gtaggtctcg
      901 cggtcggtga ccacgcggcg ggccgcaggg tcgatggaga ggatgcgctc ccgccggaag
      961 gcgacgcccg gcttcaccag ctcggcgtag cggtgcatca cctcatcgag ggtcgcacgg
     1021 ccaaacatga gctcgaactt cgagaagccg aagacgaagc cctcgttctg gtcgatcagc
     1081 gtgacgccag catcggggcc aaacgcgtcg gagaggcgag aggcgagctc gaggccgccg
     1141 aagccggcgc ccaggatcag tgtgttggtg tccatcatcg ctcctcgccg cctccaggtc
     1201 cgtgtcccga ccagtatctg cgctctccgg gacaagggct ccttcggggc tcatgacagc
     1261 catgctgccc gctcctacac tgccgccaac atctcagggc tccgccgcga ggaggccagc
     1321 catgctcgac accatgccgc tcgaaggggt gcgagtcctc gacctgaccc ggcacaccgt
     1381 gggaccgttc gcgacccggt tgctcgggga ctacggggcg gacgtgatca agatcgaggc
     1441 gcccgggggc gatccggcgc ggatgttgcc gccgttcgcg ggggatgagc cggggctcga
     1501 gcggagcggg acgttcctgt tcctgaacac gaacaagcgg agcgtggtgc tcgacctgaa
     1561 gagcgaagac gggcgggagc ggttcctgca gctcgctcgg ggcgcggacg cagtcgtcga
     1621 gaacttccga ccggggacga tggagcggct ggggctgggg tacgaggtgc tggcggggct
     1681 gaaccctcgg atcgttgtca cctcgatcag caacttcgga caggacgggc cgtaccgaga
     1741 ctgggagggg acagacctca cgatctacgc gatgggcggg ccgatgatct cgacggggga
     1801 cgtcgaccac gagccgctga aggtcgcggg gcggtgggcg gggtaccagg cggggttcgt
     1861 ggcggcgctg gcgaccgcgg tcggactgtg gggggcgcgg ctgcgcggcg agggcgagca
     1921 cctcgacgtg agcatcctgg aggcgctcac gcacaacatc gatgggcggc tgatctcgct
     1981 gctgcagtat cagtacacgg gacggccggc cgtacgacgg cctcgggcgg cgggggcggg
     2041 gaacggggtg ttcccgtgcg ccgacgggtg gtgcctgctc accggcggcg ggctggcgaa
     2101 tatgtccgcg ttcatgcgga tgatcgggca ggagcagctc ctcgaatcgc ccgagtggcg
     2161 ggacccgggc gcgttcgcgg acccggaccg gatcgaagag ttcaacgcgt acgtcctcgg
     2221 atggtcggtc atgaagacga aggccgaggt gcgggacgcg tgcgagcagt tcggggtgat
     2281 gggcgcgccg ctgaacacgg tcgccgacat gctcacggac aagtcgctcg tctaccggaa
     2341 gttcttcgag gagatcgacc acccctcgac ggggccggtg cagtacccgg gttaccactt
     2401 ccggctgcac ggcgagcacg ggccggagat gccgcctcga cggcgggcgc cgctgctggg
     2461 ggagcacacg gacgaggtgc tggcggagct ggagcaggcg acggcggcgg ttgtcgcgcc
     2521 tcgggccccg tcttcggcga ggcgggacga agcagggtac cggccgccac tggaggggat
     2581 ccggatcctc gacctcacgc tggtcctcgc gggaccgttc gcgacgatgc acctcgcgga
     2641 ctggggtgcg gaggtcatcc gcattgagtc gctgcaacac ccacagccgt tcacgcgggg
     2701 gatgccgttc gtgcggccga cgctcgacat ggtgcggcgg cagatggtgc cgccgtattt
     2761 cccggacgac gacccgggcg agcgggcgtg gaacaagccg gcgaacttca cgcaccacgc
     2821 gcgcggcaag aagtcgatga cgctcgacct gtcgcggccg gaggggcagg aggtgttcga
     2881 gcggctggtg gcacaggcgg acggggtgat tgagaacaac ctgccgcctc acatcgagaa
     2941 gctggggatc acctgggagc ggctctcgaa gatcaacccg cggctggtgc tgctgcggca
     3001 gccggcgttt ggcctcgacg gaccctaccg ggcgtaccgg acgttcggca accacatgga
     3061 ggcgatcgcg gcgcacccgg tgatccgggc gtaccccgac ctgtcgctgg actacgcgcc
     3121 gcaggggatc cccgccgacg cggcctcggg gatcgcgggg gcgtacgcgt ttctcatggg
     3181 gctgcatcag cgagagcgca ccgggaaggg ggtgatgctg gagctggcgt cggcggagaa
     3241 catcatccca ttcatgggcg agttcgtgat ggattacacc ctcaacgggc gctcatggga
     3301 gcacatgggg aacgaccatt tcttcctcgc gccgcacaac gtgtaccgct gcgcggacgg
     3361 ggaccgctgg gtcgccatcg tggcgcggaa cgaggacgaa tggcggcggc tgtgcgaggt
     3421 gatgcggcgg cccgagctgg caaacgaccc gcggttcgcc tcgatggagg cgcggtacgc
     3481 gaaccggcgc gaactcgacg cggcgatcac ggagtggacg gtgctccgcg acgcgagctg
     3541 ggtcatgcag cgcctgcagg cggagggaat cgcggcggga gtggtgatga acgaccgaga
     3601 cgtcctcgat gacccgcagc atcgagcgcg cgagttcttc gtggagatcg aggggccgga
     3661 cacggggcgg cgccagtacg tacggcgcgc atggcgggcg agcaagacgc cgtggcgcga
     3721 gatgcggcac ccggtgctgc tcggtgaaga caacgagtat atgtaccgac aactgctggg
     3781 cttcagcgac gaggagtacc gacgcttcga ggagctaggg cacatcggga cagagtaccc
     3841 ggccggcatc ccctaacggg cgcaccggcc ggaggcgcgt tcgtcggcga tggggcgcgc
     3901 tgatacgatc ggcgttccga ggaacaccca gggcagtgcc ccggtaccgg caggagccaa
     3961 cgtgcacgag cgatccgtcc gcgacgcctc gtgacggcca cgtcctcgat cgagcacagc
     4021 gggaagcggg cggcggagcc cgtcgagacg ccgctgctgg agcggctgcg ggcgcactgg
     4081 ccgctgggg
//
LOCUS       DAORPG010000103         4076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1706_length_4076_cov_3.211142, whole genome shotgun sequence.
ACCESSION   DAORPG010000103 DAORPG010000000
VERSION     DAORPG010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1706_length_4076_cov_3.211142"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PLX85_03360"
     CDS             <1..561
                     /locus_tag="PLX85_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRC62243.1"
                     /translation="DAMFGEMLVEARNLLLDWVGLDKPVVTAVRGYALGVGATLALLG
                     DVVLAAPSARFGDTHVSNQGVTAGDGGALLWPLLAGLQQARYYLLTGELVPAEEAYRL
                     GLVFRVVEDEALLDEAVAMAERLSAGAPMAVRSTKTAINRYLSLIANEILPYSIAAEV
                     ACFKSEDHLEAMAAFAERRAGRYLGR"
     gene            complement(591..899)
                     /locus_tag="PLX85_03365"
     CDS             complement(591..899)
                     /locus_tag="PLX85_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62244.1"
                     /translation="MPYTTIEVISYHLAVFSQGATIALCDAKGATFATAYVRPEAETL
                     TENHQDGTGMYRLYYHHSSLAALVDMLRNERPVYLHYWFEGGGNCHLATSLEPVGEAE
                     "
     gene            complement(1081..1857)
                     /locus_tag="PLX85_03370"
     CDS             complement(1081..1857)
                     /locus_tag="PLX85_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRC62245.1"
                     /translation="MPDLLYEKKQNYAIFTYNRPERLNALGGTLMEELHVALQDFIGD
                     PDMRAAIVTGAGRAFSAGADLKAMAERNASGQRQIALGHRGLDHSFPWVDVPKPVIAA
                     VNGLAVGGGMETAMSCDIRIASTDAYFGLFEVKRGIMAGYGIHHLMRLIPASHAYYTV
                     FTADRISPQQALQWGFVTEVVEPDRLIPRAEEIAAMIAENAPLAEAGSKAIMRSWRTY
                     GIDDSLQLNTWVSKVVIDSEDAKEGPLAFTEKRAPVWKGR"
     gene            complement(2057..2713)
                     /locus_tag="PLX85_03375"
     CDS             complement(2057..2713)
                     /locus_tag="PLX85_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62246.1"
                     /translation="MSEPPVYELVNGPDPCDICVSIAGFYPVPPDVPIHLGCECTVRT
                     LDAEGAEDVTYEVRNVEWDEEEWTEEVQGAISNCGNPQQSIDFSWGAEQTTPLEHFDP
                     GVQAAAEAAGWQRPEPQGITGHVDIAADCTLEFTFVARHVSFLFKGELWRVRLSEDPI
                     AGVVTEEEQVDEVGGIFEAMIGGELSGYTVEPCAGEDDYFQSPEESPWMEDDPREIEV
                     "
     gene            complement(2727..3737)
                     /locus_tag="PLX85_03380"
     CDS             complement(2727..3737)
                     /locus_tag="PLX85_03380"
                     /inference="COORDINATES: protein motif:HMM:NF025589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRC62247.1"
                     /translation="MTRTLLLRPLEDVLPLNATQAAALRAAGVVNVATMFERGPRRLQ
                     RVLPDVGPARLGDWYCLALLQRVPEVEAAFAAALLAAGIRTLASLAAAPADAVEAALA
                     GDATSQTPTRDEVTGFQEAANRLAATGMVAGQVVSPFGAPIEDASVRIGGENTTTNAS
                     GWFALNRVPAGVARLNIELPGGTDRVTGRPIEVAAGRFARLVQARVPATPRPLAATRR
                     DEATGSLVVSRAGLTRHLVDRPLTEFPDGAPFLVREIEAERARLLSLRRVQVGRTLLV
                     ERARVDAARLPAGAAIDSVVLLHAGALVLASQTRAELERAKLDAWRAAHPVTRRRRIA
                     IL"
     gene            complement(3849..>4076)
                     /locus_tag="PLX85_03385"
     CDS             complement(3849..>4076)
                     /locus_tag="PLX85_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62248.1"
                     /translation="PSVVFASPVAGVAVVVLAPTVGLASSAVGLTVATRVSTAVASGC
                     PPPPEHASMDRPATTANHPIIEETRMRPSLP"
BASE COUNT          584 a         1185 c         1659 g          648 t
ORIGIN      
        1 gacgcgatgt tcggcgagat gctcgtggag gcgcggaacc tactgctgga ctgggtgggg
       61 ctggacaagc cggtggtgac ggcggtgcgc gggtatgcgc tgggggtcgg tgcgacgctg
      121 gcgctgctcg gcgatgtggt gctggcagcg ccgagtgcgc gtttcgggga cacgcatgtg
      181 tcgaaccagg gagtgacggc gggcgatggc ggggcgctgt tgtggcccct gttggccggg
      241 ctgcagcagg cgcggtacta cctgctgacg ggcgagctgg tgccggcgga ggaggcgtat
      301 cgcctcggac tggtgtttcg ggtggtggag gacgaagcgc tcctcgatga ggcagtggcg
      361 atggcggagc ggctgtcggc gggggcaccg atggcggtgc ggtcgacgaa gactgcgatc
      421 aaccggtacc tgagcctgat cgcgaatgag atcctgccgt actcgatcgc ggcggaggtg
      481 gcatgcttca agagcgagga ccacctcgag gcgatggcgg cgttcgcgga gcggcgggcg
      541 gggcgttacc tcgggaggta ggcgggcccc accgacgtcg cgggaccggg ttactcggcc
      601 tcgcccacgg gttcgaggct ggtggcgagg tggcagttgc cgccgccctc gaaccagtag
      661 tgcaggtaaa ccgggcgttc gttgcggagc atgtcgacga gcgccgccag tgatgagtgg
      721 tggtaataaa ggcggtacat ccccgtcccg tcctggtggt tctcggtcag cgtctcggct
      781 tcggggcgga cgtaggcggt ggcgaaggtc gcgcccttcg cgtcgcagag agcgatggtg
      841 gctccctggg aaaagacggc gaggtggtag ctgatcacct cgatggtcgt gtagggcatt
      901 ggtgctccga tctcagcggc tgccggcgcg ctgggcgtgc gacgagagtg cacaggcagg
      961 ctggaggccg ccaaggggcg aaggtcagtc acgggggatg aggaacggcg ccgggggaca
     1021 caaagcagcc gaggcggtga gcctcggctg cttcggcgtg acctcgaagg aagttggagc
     1081 ttagcggccc ttccagacgg gggcgcgctt ctcggtgaag gcgagggggc cctccttggc
     1141 gtcctcggag tcgatgacga ccttgctgac ccaggtgttg agctgcaggg agtcatcgat
     1201 gccgtaggtg cgccacgacc gcatgatggc cttggagccg gcctcggcga gcggggcatt
     1261 ctcggcgatc atggcggcga tttcctcggc gcgcgggatc aggcggtcgg gctcgacgac
     1321 ctcggtgacg aagccccact ggagggcctg ctgcggcgag atgcggtccg cggtgaagac
     1381 ggtgtagtag gcgtgggaag cggggatgag ccgcatgagg tggtggatgc cgtagccggc
     1441 catgatgccg cgcttgacct cgaagaggcc gaagtaggca tcggtggagg cgatgcggat
     1501 gtcgcaggac atggcggtct ccatgccgcc gccgacggcg aggccgttaa ccgccgcgat
     1561 gacgggcttc gggacgtcca cccaggggaa gctgtggtca aggccgcggt ggccgagggc
     1621 gatctggcgc tggccgctgg cattgcgctc ggccatcgcc ttgaggtccg cgccggcgga
     1681 gaaggcgcgg ccggcgccgg taacgatggc ggcgcgcatg tcggggtcgc cgatgaagtc
     1741 ctggagggcg acgtgcagct cctccatgag ggtaccgccg agggcgttga gccgctcggg
     1801 gcggttgtaa gtgaagatcg cgtagttttg cttcttttcg tacagcaggt ctggcatggg
     1861 ttcccctctt tgcctgtgct cgtagggcgg ccacgcggct gcccgcctgc gtgtttctaa
     1921 cgttccgcgg gggcgggtgt caatgcgtct cgtgcgcctc ggggagaggc gaggttgggg
     1981 ccggggtagc ggtcggagcg gccagcgttt gcgacgggtg gctcctcgga gcggtgctcg
     2041 ggtgaggggc ctgctttcag acctcgattt cgcgggggtc gtcttccatc cagggggatt
     2101 cctcggggga ttggaagtag tcgtcctcgc cggcgcaggg ctcgacggtg tagccgctga
     2161 gttcgccgcc gatcatggct tcgaagatgc cgcctacctc gtcgacctgt tcttcctcgg
     2221 tgacgacgcc cgcaatgggg tcctcggaga gtcggacgcg ccagagttcg cctttgaaga
     2281 ggaagctgac atgacgggcg acgaaggtga actcgagcgt gcagtcggcg gcgatgtcga
     2341 cgtgcccggt gatgccctgg ggttcggggc gttgccagcc ggcagcctcg gccgccgcct
     2401 ggacgccggg gtcgaagtgc tcgagggggg ttgtctgctc ggcgccccag ctgaagtcga
     2461 tcgattgctg tggattgccg cagttcgaga tcgcgccctg gacctcctct gtccattcct
     2521 cctcgtccca ttcgacgttg cgcacctcgt aggtgacgtc ttcggcgccc tcggcatcga
     2581 gcgtccgcac ggtgcattcg cagccgaggt ggatgggcac gtcgggcggg acggggtaga
     2641 agccggcaat ggatacgcag atgtcgcaag gatcggggcc gttgacgagt tcgtagacgg
     2701 ggggctctga catcgatgac ccgcctctag aggattgcga tgcgccggcg gcgcgtgacg
     2761 gggtgggcgg cgcgccaggc gtcgagcttc gcgcgctcga gctccgctcg tgtctgcgag
     2821 gcgagcacca gggctccggc gtggaggagt acgacgctgt caatggcggc cccagccggc
     2881 aggcgcgcgg cgtctacgcg agcgcgttcg accaagaggg tgcggccgac ctggacgcgg
     2941 cgcaggctga gcaggcgggc gcgctccgcc tcgatctcac gaacgaggaa cggcgcgccg
     3001 tcggggaact ctgtcagcgg gcggtcgaca aggtggcggg tcaggcccgc cctggacacg
     3061 accagactgc ccgttgcctc gtcgcgacga gtggcagcca ggggacgagg ggtggcgggc
     3121 actctggcct gcacgaggcg ggcgaagcgg ccggcggcga cctcgattgg tcggccggtg
     3181 acgcggtcgg tcccgccggg gagttcgatg ttcaggcggg caacgcctgc cgggacgcgg
     3241 ttcagggcga accagccact ggcgttggtg gtcgtgttct cgccaccgat gcggaccgag
     3301 gcgtcctcga tcggcgcgcc gaagggcgag acgacctggc cggcgaccat cccggtagcg
     3361 gcgaggcggt tcgcggcctc ctggaagccg gtgacctcgt cgcgggtggg ggtctgcgat
     3421 gtggcgtcgc cggcgagtgc cgcctcgacc gcgtcggcgg gagccgcggc cagcgaagcg
     3481 agggtgcgta tgccggcggc gaggagcgcg gcggcgaagg cggcttcgac ctcggggacg
     3541 cgctggagga gcgccaggca gtaccagtcg ccgaggcgag ccggcccgac gtccggcagc
     3601 acccgctgga ggcggcgcgg gccacgctcg aacatcgtgg cgacgttcac gacgccggcc
     3661 gcgcgcaggg cagcggcctg ggtggcgttg agcggcagga catcctcgag cggacggagg
     3721 aggagggtac gggtcacggt acctccgagg cgcggagtga ggaccgttgg gtgcggggag
     3781 cgtaggcggg agcgaccgag gcggtcaact cgcggcgggt cacgtacact cgcgccgtcg
     3841 agcaggggtt acggcagaga cggacgcatg cgggtttcct cgattatcgg gtggttggcg
     3901 gtagtcgcgg gcctgtccat gctcgcgtgc tcgggcgggg gcgggcagcc ggaggccacc
     3961 gccgtcgaga cacgcgtggc gacggtcagt ccgacggcgg aactcgccag cccgaccgtg
     4021 ggtgcgagca ccacaacggc cacacccgcg acgggcgagg cgaacacgac cgaggg
//
LOCUS       DAORPG010000104         4075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1707_length_4075_cov_3.958209, whole genome shotgun sequence.
ACCESSION   DAORPG010000104 DAORPG010000000
VERSION     DAORPG010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1707_length_4075_cov_3.958209"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PLX85_03390"
     CDS             complement(<1..474)
                     /locus_tag="PLX85_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HRC62249.1"
                     /translation="MTQNTMTQSAMTQYIPALSDVRAMAGRGNIVPIYREVRADLETP
                     VSAYLKVARGSYSFLLESVEGGERVGRYSFIGTEPYRVISREFEDGDPLDEVEAELGR
                     FKSVDVPGLPRFHGGAVGYLGYEVVRHFEPRVPAPDRDVLGVPESWLMFTDHLLVF"
     gene            complement(642..1700)
                     /gene="trpS"
                     /locus_tag="PLX85_03395"
     CDS             complement(642..1700)
                     /gene="trpS"
                     /locus_tag="PLX85_03395"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRC62250.1"
                     /translation="MVFFVPVRGRQSEEYVARQRILTGMRPTGPLHLGHYVGALENWL
                     ELQHEYDCYFLIADYQALGDHLDEIERIRRSVIDVAVDWLSVGLDPERSPFVVQGYVP
                     EHAELTLYFSMLTPKSWLDRNPTLKSEMQQLEDSHETITMGFFNYPLSQAADILLPKA
                     NLVPVGEDQLPHIELTREIARRFNRQYGEIFPEPQAKVGRVPRLLGLDGQAKMSKSLD
                     NAIYLSDDAKTVQKKVRRMVTDITGQNPRLSPTDPGIVENNPVFLYHDVFNPDVEEVK
                     DLKERYEQGKVSDGEVKDKLVAALNAFLDPIRERRAQILERPGYAAEVLEEGSRRERV
                     IAQETIAEVREAMRLVYT"
     gene            2215..3093
                     /locus_tag="PLX85_03400"
     CDS             2215..3093
                     /locus_tag="PLX85_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62251.1"
                     /translation="MTRNQLLTVFAGLVMAIAGFALSSDSASAIAASQANVGYGKCWQ
                     NGSGNCYHWARTSQGLTLRVESDVTGGWSTYFNRAIADWTFAIDAPVTAGTELVPFSM
                     VGQNSYKTSASCGGITGLTRVCNKAYGLNGWLGLASIWVAADGEHITAGTVKLNDSYF
                     GTAAYDTPAWRNLVSCQELAHTLGLAHQDETFGNANLGSCMDYTNDPSTNQYPNDDDR
                     KTLANMYAHGDSTSTVSAIGSGSAKNGAGQGLPDEDALPPNAKPSQGDVFVKRLPDGG
                     TLITHVFWVERGSPGR"
     gene            complement(3103..3570)
                     /locus_tag="PLX85_03405"
     CDS             complement(3103..3570)
                     /locus_tag="PLX85_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRC62252.1"
                     /translation="MATTTEERTGIRSATLLGVGIAFAAAASYGSSQVLTRQGVSDLA
                     PPLVGSFIALFWGTLGFLVISLRSLRGVRGDLKTGGLYFAAAGAFSATGVILMFQALS
                     RGEVVVISPVLATNPLFTLVLAAVFLRGVERITAPTVIGAVLVVTGVAVLSIA"
     gene            complement(3574..>4075)
                     /locus_tag="PLX85_03410"
     CDS             complement(3574..>4075)
                     /locus_tag="PLX85_03410"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRC62253.1"
                     /translation="CSHRVCQSLPLAGTRRGIIEAGDAMFAIVLALVAAACWGLSAVL
                     VRTGLRYLTPTVGTLISLVSALALTAVLVVALQLPELLDVSLQAVAMFALIGILNFPM
                     GRFLNYLAMSHLGVGRSTPLLASAPLFAVLGAVVFTGEELHLATVAGIGLIIGGLYVT
                     LTAKTG"
BASE COUNT          698 a         1299 c         1449 g          629 t
ORIGIN      
        1 gaacacgagc aggtggtcgg tgaacatgag ccacgactcg ggcacgccga gcacgtcgcg
       61 gtcgggagcg ggcacgcgcg gttcaaagtg acgcaccacc tcgtagccga ggtagccgac
      121 ggccccaccg tggaaacgcg gcaggcctgg tacgtcgacc gacttgaagc ggccgagctc
      181 tgcctcgacc tcgtcgaggg ggtcgccatc ctcgaactcc cgcgagatga cgcggtacgg
      241 ctcggtgccg atgaaggagt agcgccccac gcgctcgcca ccctcgacgg actcgagcag
      301 gaacgagtac gagccgcggg cgaccttcag gtaggcggac acgggcgtct cgaggtcggc
      361 gcgcacctcg cggtagatgg ggacgatgtt gccgcgtccg gccatggcgc ggacgtcgga
      421 gagagccggg atgtactgag tcatcgcgct ctgggtcatc gtgttctggg tcatcgggag
      481 cctcgacttt cggtcggaac ggacatggat ggatggccgg cggagctgcg ccagcaccgg
      541 ccgtgccagg accagcgtcc gagcgatgcc gtgtgcgtgc gcgctgccag cggcctgcgt
      601 cccatgcctc gacgctcctt tcgctcgccc gggcgagctg cctacgtgta cacgaggcgc
      661 atggcctcgc ggacttcggc gatcgtttcc tgcgcgatca cgcgctcgcg gcggctgccc
      721 tcctcgagca cctcagcggc gtagccgggg cgctcgagga tctgggcgcg tcgctcgcgg
      781 atcgggtcga ggaaggcatt cagagcggcg acgagcttgt ccttcacctc gccgtcgctc
      841 actttgccct gctcgtaacg ctccttgagg tccttcacct cctcgacgtc cgggttgaag
      901 acgtcgtggt acaggaagac cgggttgttt tcgacgatgc cggggtcggt ggggctgagg
      961 cgcgggttct gccccgtaat gtcggtgacc atccggcgga ccttcttctg gaccgtcttc
     1021 gcgtcgtccg agaggtagat ggcgttgtcc aggcttttgc tcatcttggc ctggccatcc
     1081 aggccgagca ggcgcgggac gcggccgacc ttcgcctggg gctcggggaa gatctcgccg
     1141 tactgccggt tgaagcggcg cgcgatctct ctcgtcagct cgatgtgagg gagctgatct
     1201 tcgcccaccg ggacgaggtt cgccttcggc agcaggatgt ccgcggcctg gctcagcggg
     1261 tagttgaaga agcccatcgt gatggtttcg tgggaatcct cgagctgctg catctcgctc
     1321 ttgagcgtcg gattgcggtc cagccagctc ttcggggtga gcatgctgaa gtacagcgtc
     1381 agctccgcgt gctccgggac gtagccctgc acgacgaacg gcgaccgctc ggggtcgaga
     1441 ccgacggaga gccagtcgac ggcgacgtcg atgacggagc gccggatgcg ctcgatctca
     1501 tcgaggtggt cgccgagcgc ctggtagtcg gcgatcagga agtagcagtc gtactcgtgc
     1561 tgcaactcga gccagttctc gagcgcgccg acgtagtgac cgaggtggag cggccccgtc
     1621 ggccgcatcc ccgtcagaat tcgctgtcgc gccacgtact cctccgactg ccgcccgcga
     1681 acgggcacaa aaaagaccat cgcctggggc gatggtcctc gcggtgccac ccaagtgccg
     1741 cgcgggactc gatggccacg cgcgcttatt caaggcacgg ccgccgccga agcggagcga
     1801 tgccctgggc ctggataacg gcgcccacac cgccggcgcc tactggccgg cttctcgagt
     1861 tgcctcgatg gccggcggtt cgggccggga ctcccgggtc cattcaaccg ttgctggacg
     1921 ggtaccgggc tcgcaccttg ccccggctct ctgcacccac ggtgtgggaa accccgctgg
     1981 acggcctact agtcccgttc ggcgtctgta cgtattcgta acgacgttgc ttcaacggta
     2041 gggcgaggcg gcgagcgctg tcaacgtcgc gtaaggcggc gccggggcgt tagtgcgctt
     2101 ccgcccggcg gactggctgc taggctgaaa cggcgctcac ccgatcgagg attgaacgag
     2161 cggatcaggc tgttgcgaag tagggagcag cctgactgcc cgaggaggtt cgaggtgact
     2221 cgaaatcagc tattgacggt gttcgcgggc ctcgtgatgg cgatcgccgg gttcgcgttg
     2281 tcctcggatt cggccagcgc gatcgccgcg agccaggcca acgtgggata cgggaagtgc
     2341 tggcagaacg ggtcgggcaa ctgctaccac tgggcgcgca cctcacaggg gctgacgttg
     2401 cgggtggaaa gcgacgtgac gggcggctgg agcacgtact tcaaccgggc catcgcggac
     2461 tggaccttcg ccatcgacgc gccggtaacc gcgggcaccg agctggtccc gttctcgatg
     2521 gtcgggcaga acagctacaa gacgagcgcc agctgtggcg gcattacggg tctgacgagg
     2581 gtctgcaaca aggcctacgg cctgaacggc tggctcggcc tggcctcgat ctgggtggcg
     2641 gctgacggcg agcacatcac ggcaggcacc gtgaagctga acgactcgta cttcgggacg
     2701 gcggcgtacg acacgccggc gtggcgcaac ctggtgtcct gccaggaact ggcgcacacc
     2761 ctcgggctgg cgcaccagga tgagacgttc gggaacgcga acctcggttc gtgcatggac
     2821 tacacgaacg acccctcgac gaaccagtac ccgaacgacg acgaccgcaa gacgctcgcg
     2881 aatatgtatg cgcacggcga cagcacgagc acggtgagcg cgatcggcag cgggagcgcc
     2941 aagaacggcg ctggtcaggg cctaccggac gaggacgcgc tgccgccgaa cgcgaagccg
     3001 tcacagggtg acgtgttcgt gaagcggctg ccggacggtg gcacgctgat cacgcacgtt
     3061 ttctgggtgg agcgtggcag tccgggccgg tagacctcgc gctcaggcga tgctgagcac
     3121 ggccacgcca gtgaccacga gcacggcgcc gatcacggtc ggcgccgtga tgcgttccac
     3181 gccgcgcagg aagaccgccg cgaggacgag cgtaaacagc gggttggtgg ccaacaccgg
     3241 ggagatcacg acgacctcgc cacgactcag ggcctggaac atgaggatca cgcccgtggc
     3301 cgagaaggcc ccggcggccg cgaagtagag tccgcccgtc ttcaggtcgc cccgtacgcc
     3361 ccgcagggag cggagcgaga tgacgaggaa ccccagcgtc ccccagaaca gcgcgatgaa
     3421 cgagccgacc agcggcggcg cgaggtcgga cacgccctgg cgggtgagca cctgggatga
     3481 gccgtacgaa gccgcggcag cgaaggcgat gccgaccccg agcagggtcg cactacggat
     3541 tccggtgcgt tcctcggtgg tggtcgccat gtctcagcct gtctttgcgg tcagggtgac
     3601 gtagaggccg ccgatgatga gcccgatgcc ggcgacggtc gccaggtgca actcctcgcc
     3661 ggtaaagacc accgctccga gcaccgcgaa cagcggtgcg gaggcgagca acggcgtcga
     3721 acggccgacg ccgaggtggc tcatcgccag gtagttcagg aagcggccca tggggaagtt
     3781 caggatgccg atgagcgcga acatcgcgac ggcctgcagg gagacgtcga ggagttccgg
     3841 cagttgcagc gcgacgacca gcaccgccgt gagcgccagc gccgacacga gcgagatgag
     3901 ggtacccacc gtcggggtca ggtagcggag gccggtgcgc acgagcaccg cgctgaggcc
     3961 ccagcaggcc gctgcgacca gggcgaggac gatcgcaaac atggcgtctc ctgcctcgat
     4021 aatgccgcgc cgggtcccgg ccaaggggag gctctggcat actcgatgcg aacac
//
LOCUS       DAORPG010000105         4074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1709_length_4074_cov_3.148047, whole genome shotgun sequence.
ACCESSION   DAORPG010000105 DAORPG010000000
VERSION     DAORPG010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1709_length_4074_cov_3.148047"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="PLX85_03415"
     CDS             complement(<1..671)
                     /locus_tag="PLX85_03415"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRC62254.1"
                     /translation="MAKIKVANPVVELDGDEMTRIIWGFIKERLILPYLDIDLRYYDL
                     SIQSRDATNDQITVDAAEAIKQYGVGVKCATITPDEARVEEFGLKEMWKSPNGTIRNI
                     LGGTVFREPIICRNVPRLVPGWTDPIVIGRHAYGDQYRATDFVVPGPGKLTVTFTPQD
                     GSPPIQRDVFDFPGGGIALTMYNLDESIRGFARSSFNYGLARGWPGYLSTKNTILKAY
                     DGRFK"
     gene            complement(719..2266)
                     /locus_tag="PLX85_03420"
     CDS             complement(719..2266)
                     /locus_tag="PLX85_03420"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014556696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRC62255.1"
                     /translation="MSFEEKLEYLAALKQRASLGGGQDRIDAQHQRGKLTARERIAML
                     VDPGTFEEIDALAVEWNENESDGERFYGDAVVTGWGRVDGRPVFVFAQDFTLFGGSLS
                     EVMGEKISKMMDHAASTGAPVIGLNDSGGARIQEGVGSLAGYGDIFFRNVRTSGVIPQ
                     ISVIMGPCAGGAVYSPAITDFIFMVEGTSQMYITGPDVIQSVTGEKTTFEELGGAMSH
                     AAKSGVAQFSFADEESCLAEVKRLLSFLPSNNMEDPPVVDTGDDPTRRLDDILNIVPD
                     EETLPYDVRDIIERLVDNGDFMEVHQYFAQNIVVGLGRIGGRSVGIVANQPMYLGGVL
                     DIDSSRKAARFVRFCDSFNIPIVTLVDVPGYLPGIAQEYGGIISHGAKVLYAYAEATV
                     PKVTVITRKAFGGAYVAMGSKHLGVDINYAWPGAAIAVMGGGQAVNIINRREIQAAAD
                     PDATRADLVADYKQRLEHPYIAAARGYVDDVIEPRDTRAKIAHALEMLRHKSEALPAK
                     KHGNIPL"
     gene            complement(2349..3011)
                     /locus_tag="PLX85_03425"
     CDS             complement(2349..3011)
                     /locus_tag="PLX85_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRC62256.1"
                     /translation="MRRLFALTAAALLTFVVACGGGSSPKQADDSNAPVASATPYAVL
                     PNVILVTGNGSGGGTSGNTGGTNGGGAGTSGGATNEVKYVVQSGDTLLALANRYGTSV
                     EAIMTRNNIASAADLRAGQEIVIPSGSSSVATPTATPARTATPASTATAVATPRPTGT
                     ATATGTATGGQRYTVQSGDLAGSIAARFGVTLDQLAAANNRTVQSLDQLNVGDVLIIP
                     GR"
     gene            complement(3058..>4074)
                     /locus_tag="PLX85_03430"
     CDS             complement(3058..>4074)
                     /locus_tag="PLX85_03430"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HRC62257.1"
                     /translation="EYIQQQLQAAGYRVTLEEFDVQATLGGNATLTVGGGASVETAPL
                     SGSANGSVSGQVVPAGLGRASDLAAAGVSGKIALLDRGEITFAEKVRNAQAAGATAVI
                     IANNEAGMFRGDLGGEPVRIPVVSVARESRDAVAAGGAEVHIEVQTNRVSGRSQNVVA
                     KPSDAPCTSYLGAHYDSVPAGPGANDNASGTSLLIELARTRRVDGVCIVAFGGEEVGL
                     FGSKAFVRQHDVSTAAFMMNFDMVAKATRPTFIGDEALTKIAAQVAGERGVTARSIAS
                     FGPGTSSDHASFQAAGVPVLMFYAGDDEHIHTAQDNLDNVSEGDLAKLLDVAVAVLDR
                     LLAG"
BASE COUNT          590 a         1386 c         1369 g          729 t
ORIGIN      
        1 tccttgaaac gcccgtcgta ggccttgagg atggtgttct tcgtcgacag gtaccccggc
       61 cagccgcgcg cgagcccgta gttgaaggac gagcgcgcga acccgcggat cgattcgtcg
      121 aggttgtaca tcgtgagcgc gatgccgccg cccgggaagt cgaagacgtc ccgctggatc
      181 ggtgggctgc cgtcctgcgg cgtaaacgtc acggtcagct tcccggggcc aggcaccacg
      241 aagtccgtgg cgcggtactg gtcgccgtag gcgtgacgtc cgatgacgat cgggtcggtc
      301 cagcccggaa ccagccgggg cacgttgcgg cagatgatcg gctcgcgaaa gaccgtcccg
      361 ccgaggatgt tgcggatggt cccgttgggg gacttccaca tctccttgag gccgaactcc
      421 tcgacgcgcg cctcgtcggg cgtgatggtg gcgcacttca caccgacgcc gtactgcttg
      481 atcgcctcgg ccgcgtcgac cgtgatctgg tcgtttgtgg cgtcgcggct ctggatgctg
      541 aggtcgtagt agcggaggtc gatatcgagg taggggagga tcagccgctc tttgatgaat
      601 ccccagatga tccgggtcat ctcgtcgccg tcgagttcga cgaccgggtt cgcgactttg
      661 atcttcgcca tgctgctacc ttcgattccc gtcccccgca cgaaggcgcg agggcgtcct
      721 acagcggaat gttcccgtgc ttcttcgccg gcaacgcctc cgacttgtgt cgcagcatct
      781 cgagggcgtg cgcgatcttg gctcgcgtgt cacgcggctc gatcacgtcg tccacatagc
      841 ctcgggccgc cgcgatgtac gggtgctcga gccgctgctt gtagtcggcg acgaggtcgg
      901 cgcgcgtcgc gtccgggtcg gccgcggcct ggatctcccg gcggttgatg atgttcaccg
      961 cctggccgcc gcccatgacc gcaatcgccg cccctggcca ggcgtagttg atatcgacgc
     1021 cgaggtgctt cgaacccatc gcgacgtacg cgccgccgaa cgccttgcgc gtgatcacgg
     1081 tgaccttcgg caccgtcgcc tcggcgtagg cgtagagcac cttggcgccg tgcgagatga
     1141 tcccgccgta ctcctgcgcg atgccgggga ggtagcccgg cacatcgacc agcgtcacga
     1201 tcgggatgtt gaacgagtcg cagaagcgca cgaagcgtgc cgccttgcgc gacgagtcga
     1261 tgtcgagcac gccgccgagg tacatcggct ggttcgcgac gatcccgacg cttcgcccac
     1321 cgatgcggcc gagcccgacc acgatgttct gcgcgaagta ctggtggact tccatgaagt
     1381 ccccgttgtc gaccaggcgc tcgatgatgt cgcgcacgtc gtacggcaac gtctcttcgt
     1441 ccggcacgat gttgagaatg tcgtcgagcc tgcgagtggg gtcgtcgccc gtgtcgacga
     1501 ccggcgggtc ctccatgttg ttcgacggca ggaacgagag cagccgcttc acctcggcga
     1561 ggcaggattc ctcgtcggcg aacgagaact gcgcgacgcc cgacttcgcg gcgtgggaca
     1621 tcgcgccacc gagctcctcg aacgtggtct tttcaccggt gacgctctga atgacgtccg
     1681 gcccggtgat gtacatctgc gaggtgccct cgaccatgaa gatgaagtcg gtgatcgccg
     1741 gcgagtagac ggcgccgccc gcgcagggcc ccatgatcac gctgatctgg ggaatgaccc
     1801 ccgaggtgcg cacgttgcgg aagaagatgt cgccgtagcc ggcgagcgag cccacgcctt
     1861 cctggatgcg cgccccgccg gagtcgttca agccgatcac gggagcaccg gtgctcgccg
     1921 cgtggtccat catcttcgaa atcttctcgc ccatgacttc ggacagggag ccgccgaaca
     1981 gcgtgaagtc ctgggcgaag acgaagaccg gccgtccatc gacccggccc cagccggtga
     2041 cgacggcgtc gccgtagaag cgctcgccgt cgctctcgtt ctcgttccat tcgaccgcga
     2101 gtgcgtcgat ttcctcgaag gtgccggggt cgacgagcat cgcgatgcgc tcgcgtgcgg
     2161 tcagcttgcc tcgctggtgc tgcgcatcga tccggtcctg gcccccgccg agcgaggctc
     2221 gctgcttcag cgccgccagg tactcgagct tctcttcgaa cgacatctag gctcactctc
     2281 cggtgtccag agtgtgtgga cgatctcggg agagtatcgg ccggatcgcc ccacggcgcc
     2341 agcgcgcgct aacggccggg gatgatcagg acgtcgccga cgttcagctg gtcgaggctc
     2401 tgtacggtcc ggttgttcgc ggctgcgagc tggtccagcg tcacgccaaa gcgagccgcg
     2461 atcgagcccg cgaggtcgcc gctctgcacg gtgtaccgct gcccgcccgt ggcggtaccg
     2521 gttgctgtgg cggtgccggt tggccggggc gtggcgacag cagtggccgt cgaggcggga
     2581 gtcgcggtgc gcgccggggt ggccgttggc gtcgcgaccg agctcgatcc gctcgggatg
     2641 acgatctcct gtccggcgcg gaggtcggcc gcgctcgcga tgttattgcg ggtcatgatc
     2701 gcttcgaccg aggtgccgta gcggtttgcg agggccagca gggtgtcccc ggactgcacg
     2761 acgtacttca cctcgttggt ggcgccgccg cttgtcccgg ccccgccccc gttggtgccg
     2821 ccggtattac cgctcgtgcc gccgccgctt ccgtttcccg tgacgaggat cacgttcggc
     2881 agcaccgcat atggcgtcgc gctggcgacg ggcgcgttcg agtcgtccgc ctgcttcggg
     2941 ctgctgccgc ccccgcaggc gaccacgaac gtcaggaggg cggcggcggt cagggcgaag
     3001 agtcgtcgca tgggcgtccg tatcgagggg gaagctgcgg tcatcgtagc cgagcgctca
     3061 tcccgcgagc aatcggtcga gcaccgcgac cgccacgtcg agcagcttcg ccagatcccc
     3121 ttcggagacg ttgtcgaggt tgtcctgtgc cgtgtggatg tgctcatcgt cacccgcgta
     3181 gaacatcagc accggcacgc ctgccgcctg gaacgacgca tggtccgagg acgtcccggg
     3241 gccgaacgag gcaatcgaac gcgccgtgac gcctcgctcg cccgcaacct gcgccgcgat
     3301 cttggtgagc gcctcgtcgc cgatgaacgt gggtcgcgtc gccttcgcga ccatgtcgaa
     3361 gttcatcatg aaggcggcgg tcgatacgtc gtgctgtcgt acgaacgcct tcgagccaaa
     3421 gaggcccacc tcctcgccgc cgaacgccac gatgcagacg ccatccaccc ggcgcgtccg
     3481 tgccagctcg atcagcagtg acgtgcccga ggcgttgtcg ttcgcgccgg ggcccgccgg
     3541 caccgagtcg tagtgtgccc cgaggtagga ggtgcagggc gcgtcgctcg gctttgcaac
     3601 gacgttctgc gagcgtccgc tcacgcggtt cgtctgcacc tcgatatgga cctcggcccc
     3661 tcccgcggcc accgcatcgc gcgactcgcg cgcgacgctc acgacgggta ttcgcaccgg
     3721 ctcaccgccg aggtcgccgc ggaacatccc cgcctcattg ttcgcgatga tgaccgcggt
     3781 cgcgccggcc gcctgcgcgt tccgcacctt ctccgcgaac gtgatctcgc cccgatcgag
     3841 caacgcgatc ttgccgctca ctccggccgc cgcgagatcg ctcgcgcgcc cgaggccggc
     3901 gggtacgact tgcccgctga cgctgccatt cgccgacccg ctcagtggcg ccgtctcgac
     3961 gctcgcgcca ccgccgacgg tcaatgtggc gtttccgccc agggtggcct ggacgtcgaa
     4021 ctcctcgagg gtgacccgat aaccggccgc ctgcagctgc tgctggatgt actc
//
LOCUS       DAORPG010000106         4073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1710_length_4073_cov_3.156297, whole genome shotgun sequence.
ACCESSION   DAORPG010000106 DAORPG010000000
VERSION     DAORPG010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1710_length_4073_cov_3.156297"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /gene="argH"
                     /locus_tag="PLX85_03435"
     CDS             <1..1002
                     /gene="argH"
                     /locus_tag="PLX85_03435"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRC62258.1"
                     /translation="TARLRALRRALLDLAVREAETVISGYTHLQRAQPVLLAHHLLAY
                     EAMFARDSERFEQVRQRTNRLPLGAGALAGVTYPIDREWVAQELGFDSVIPNSLDAVS
                     DRDFVVEIHAASALAMAHLSRLSEEVVLWSTAEFGLIQLPDAFATGSSIMPQKKNPDV
                     AEIVRGKSARAIGNLVQSLTLLKGLPLSYNRDLQEDKEPLFDTVDTLLSSLAVTAAML
                     PALHFDAERGRAMAVGQFALATDVADYLAKRGVPFREAHAAVGALVAKCERDGRRLEE
                     LTLDEYRAAHPAFEADVLAIDLDAALRARSATGGTAPEAVAHERASARARLDAEAAS"
     gene            999..1673
                     /gene="rpe"
                     /locus_tag="PLX85_03440"
     CDS             999..1673
                     /gene="rpe"
                     /locus_tag="PLX85_03440"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRC62259.1"
                     /translation="MTSVRRALIAPSILTADFGRLAEQVRAAEEGGCDLFHLDVMDGH
                     FVPQLSFGPSVVGAVRAATTLPLEVHMMVERPHEHFESFAREGAQRLVFHYEAAGDPA
                     EHLELAHRLGCEAGIAINPETPVEAIVPWLAALDEVVVMLIKPGRGGQEMMPEHLEKV
                     RDLRARVGGLGREFPIEVDGGVKSHNATTCVEAGATILVAGSAVYNERETPQQALAAL
                     RSALGD"
     gene            1673..2422
                     /locus_tag="PLX85_03445"
     CDS             1673..2422
                     /locus_tag="PLX85_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009882543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRC62260.1"
                     /translation="MPSGAEAVAQADELERELRRLGRPERAASERRYLKSDLEFIGVD
                     AAGMRSVLRAYLQARDRLDHDALVELVGALWDRPIHERRALAVSLLERQRRLLGAEDL
                     PLLERLLRDALTWAYVDALAVHVVGWLVERHPELSATLDRWAADPDFWIRRSALLALL
                     PPLRRGTGDFERFARYADAMLEEREFFIRKAIGWVLREASKSQPELVYAWVAPRTHRT
                     SGVTIREAVKYLPKAQRAALLAAYTEKRPTA"
     gene            complement(2485..2706)
                     /locus_tag="PLX85_03450"
     CDS             complement(2485..2706)
                     /locus_tag="PLX85_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL28 family ribosomal protein"
                     /protein_id="HRC62261.1"
                     /translation="MPSGTCDFCNKTVAFGRNIRHQHSGRWERKAPRTNRQFRPNVQS
                     RRMLLADGRRQRMNICTRCLRTQMKLAVS"
     gene            2816..>4073
                     /locus_tag="PLX85_03455"
     CDS             2816..>4073
                     /locus_tag="PLX85_03455"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HRC62262.1"
                     /translation="MNTTDATSLSTSALRDALHTAARWLQRNRDAINAINVYPVPDGD
                     TGTNMLLTFRAALDAGDRAGAVPAGEYMAAFSRGALLGARGNSGVILSQMFRGAALAL
                     EGVEVVDGDALRRALSTAATTAYEAVSAPVEGTMLTVMREAASGAAGDDLVTVLRTAG
                     EAVRASVARTPELLPRLREAGVVDSGGLGIAVILAGLRMAVLGEALPDPPATPQGAVE
                     LSAVAHDGHGYCTEYLVERRDGVPLDRGAMLTALESTGGDSILVVGDPDALHVHLHLE
                     DPGPGLSVGVHFGLLRDIKIDNMQLQHEAWSAAHRAGAVDGRSSDRALGLVAAATGPG
                     IAAAFRELGALPLLDGAKPTPATFLEAARSTGSKRVFLLPNHVDAIMAAEQAAREEPE
                     LVRVIPARSIPAGIAAAVAYSPDGDAA"
BASE COUNT          603 a         1397 c         1442 g          631 t
ORIGIN      
        1 accgcgcgat tgcgcgcgct gcgccgcgcg ctcctcgacc tcgcggtccg cgaggcggaa
       61 accgtgatct cgggctacac gcacctgcag cgcgcgcagc cggtcctgct cgcacaccac
      121 ctgctcgcct acgaggcgat gttcgcgcgc gacagcgagc ggttcgaaca ggtccggcag
      181 cgcacgaacc ggctgccgct gggggcgggc gccctggcgg gcgtcacgta ccccatcgat
      241 cgagagtggg tggcgcagga gctgggattc gactcagtca tcccgaactc cctcgatgcg
      301 gtctcggacc gcgacttcgt ggtcgagatc cacgcggcgt cggctctcgc gatggctcac
      361 ctgtcgaggc tgagcgagga ggtcgtgctg tggtcgaccg ccgagttcgg tctcattcag
      421 ttgcccgacg cgtttgcgac cgggtccagc atcatgccgc agaagaagaa cccggacgtc
      481 gccgagatcg tgcgaggcaa gagcgcccgc gccatcggca acctcgtcca gtcgctcacc
      541 ctgttgaagg gcctgccgct ctcctacaac cgcgacctgc aggaggacaa agagccgctc
      601 ttcgacacgg tcgacacgct gctttcctcg ctcgccgtga ccgcggccat gctgccggcg
      661 ctgcacttcg acgctgagcg aggacgtgcg atggccgtcg gccagttcgc gctggccacg
      721 gacgtggccg actatctggc gaagcgcggc gtgccgttcc gcgaggcgca cgcggccgtc
      781 ggcgctcttg tcgcgaagtg cgagcgcgac ggccgccggc tggaagagtt gacgctcgac
      841 gagtaccgcg cggcgcaccc ggcattcgaa gcggatgtcc tcgcgatcga cctcgatgcc
      901 gcactgcgag ctcgttccgc gaccggcggt accgcaccag aggccgtggc gcatgaacgc
      961 gcgtcggcgc gagcgcgact cgatgccgag gccgcctcgt gacgagtgtc cgccgcgcgc
     1021 tgatcgcgcc ctcgattctg accgccgact tcggccgact cgccgagcag gtgcgggcgg
     1081 ccgaggaggg cggctgcgat ctcttccacc tcgacgtgat ggacgggcat ttcgtgccgc
     1141 agctgagctt tgggccgtcg gtggtcgggg cggtgcgagc ggcgaccacg ttgccgctcg
     1201 aggtgcacat gatggtcgaa cgcccgcacg agcacttcga gtcgttcgct cgcgagggcg
     1261 cgcagcggct ggtctttcac tacgaggccg caggcgatcc cgctgagcac ctcgagctcg
     1321 cgcaccgcct cggttgtgaa gccggcatcg ccatcaatcc tgagacgccg gtggaagcga
     1381 tcgttccatg gctcgccgcg ctcgatgagg tcgtcgtgat gctgatcaag cccgggcgcg
     1441 gaggccagga gatgatgccg gagcacctgg agaaggtgcg cgacctgcgg gctcgcgttg
     1501 ggggcctcgg acgcgagttc cccatcgagg tcgatggggg cgtgaagtca cataacgcca
     1561 cgacgtgcgt cgaggccggt gcgacgatcc tcgtcgcagg gtcggccgtc tacaacgagc
     1621 gcgagactcc acagcaggcg ctcgccgcgt tgcgttccgc gctgggcgac tagtgccctc
     1681 cggggcggag gcggttgcgc aggccgatga gctggagcgc gagctgcggc ggctgggccg
     1741 cccggagcgc gccgcgagcg aacgccggta cctcaagagc gacctggaat tcattggcgt
     1801 cgacgcggcc gggatgcgtt cggtgctgcg tgcctacctg caggctcgcg accgtctcga
     1861 ccacgacgcg ctcgtcgaac tggtcggcgc actctgggat cgtcccatcc acgagcgtcg
     1921 cgcgctggcc gtgagcctgc tcgaacgtca gaggcgcctg cttggagccg aggatctgcc
     1981 gctgctcgaa cgactgctgc gcgacgcgct cacctgggcg tacgtggacg cgctggccgt
     2041 acacgttgtg ggctggctgg tcgagcgcca tcccgagctg tccgcgaccc tcgatcgctg
     2101 ggctgcggac cctgacttct ggatccggcg ctccgcgctg ctcgcgctgc tgccacccct
     2161 gcgccgcggc acgggcgact tcgaacgttt cgcccgctac gcggacgcga tgctcgagga
     2221 gcgcgagttc ttcattcgca aggcgatcgg ctgggtactt cgggaggcct caaagtcgca
     2281 gccggagctg gtgtacgcct gggtcgcgcc gaggacacac cgtacctcgg gcgtcacgat
     2341 ccgggaggcc gtgaagtacc tgccgaaggc gcagcgggcg gcgctcctcg cggcgtatac
     2401 cgagaagcgg cccacggctt gaggtagatg cgaaacgaaa aacgccccgc ggagcggggc
     2461 gtcatccttg gtctgccgcg tctcctagga gacggcgagc ttcatctggg tgcgcaggca
     2521 gcgcgtgcag atgttcatgc gctggcggcg accgtcagcg aggagcatgc ggcgcgactg
     2581 cacgttcggc cggaactgac ggttcgtgcg cggagccttc cgttcccagc gaccggagtg
     2641 ctggtggcgg atgttgcggc cgaaggcgac cgtcttgttg cagaaatcac acgtccctga
     2701 gggcatacga accgtcccca aacggatgca ggcgcatccg gctgcttcgg atagagtagt
     2761 agcggtaagc ctagcatcgc cgcgcgccct gcgtccaacc cgaacgagcg agcagttgaa
     2821 cacgaccgac gccacatcgc tctcgacgag cgcgctgcga gatgcgttgc acactgcagc
     2881 ccgctggcta cagcggaacc gcgacgcgat caacgcgatc aacgtgtacc cggtgcccga
     2941 tggcgacacc ggtacgaaca tgctgctcac cttccgggcc gccctcgacg ccggagaccg
     3001 cgccggcgcg gttcccgcag gcgagtacat ggccgccttc tcgcgcggag cgctgctggg
     3061 cgcgcgcggc aacagcggcg tcatcctgtc ccagatgttc cgcggcgctg cgttggccct
     3121 cgaaggagtc gaggtcgtcg acggagatgc gcttcgacgt gcgctgagca cagcggcaac
     3181 gacagcctac gaggccgtga gcgcgcccgt cgaggggacg atgctgaccg tgatgcgcga
     3241 ggctgcgtcc ggcgcggcag gcgatgacct cgtcacggtg ctgcggacgg cgggggaggc
     3301 ggttcgggcg agcgtcgctc gcacgcccga gctgctgccg cgactgcgcg aagccggcgt
     3361 ggtcgactcg ggcgggctgg gcatcgccgt catcctcgcg ggtctgcgta tggccgtcct
     3421 cggtgaagcg ctgccggacc cacccgcgac cccgcagggc gcggtcgagc tctcggccgt
     3481 cgctcacgat gggcacgggt actgcaccga atacctggtc gagcggcggg acggcgtacc
     3541 gctcgaccgc ggcgcgatgc tgacggcgct cgaatcgacc ggtggcgact cgattctcgt
     3601 cgtcggcgac cccgatgcgc tgcacgtgca tctacacctc gaggaccccg gacctggact
     3661 ctcggtcggc gtccatttcg gactgctccg cgacatcaag atcgacaaca tgcaactgca
     3721 gcacgaggcg tggtcggccg cgcatcgtgc cggtgcggtg gatggacgct cctcggatcg
     3781 cgcgctcggc ctggtggcag ctgccaccgg ccccggtatc gccgccgcct tccgcgagct
     3841 cggcgctcta ccgctgctgg acggcgccaa accgaccccc gcgacgttcc tcgaggccgc
     3901 acggtcgacc gggagcaagc gtgtctttct gctgccgaac cacgtggacg cgatcatggc
     3961 cgccgagcag gccgcccgcg aggagccgga gctcgtgcgg gtgatcccgg cgcgctcgat
     4021 ccccgcgggc atcgccgccg ccgtcgctta ttcgccggac ggcgacgccg ccg
//
LOCUS       DAORPG010000107         4059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1721_length_4059_cov_3.387363, whole genome shotgun sequence.
ACCESSION   DAORPG010000107 DAORPG010000000
VERSION     DAORPG010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1721_length_4059_cov_3.387363"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..417
                     /locus_tag="PLX85_03460"
     CDS             91..417
                     /locus_tag="PLX85_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HRC62263.1"
                     /translation="MSHAEQASEVSEQHGLTVRQYLMIGAALTVITIIELAVSYSDLG
                     SAMVPILLALSAVKFVVVVAYFMHLRFDSALFTRMFVGSFLLALAILVALVSLFWADS
                     HTLPLK"
     gene            complement(528..1481)
                     /locus_tag="PLX85_03465"
     CDS             complement(528..1481)
                     /locus_tag="PLX85_03465"
                     /inference="COORDINATES: protein motif:HMM:NF021214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HRC62264.1"
                     /translation="MDADLLGQLVSTGTAAHDTVREGHSVWTAWTLDLPVILPACIVG
                     FFYARGLRRWTVRHRQHPWWKVASYYLGLGSLVLAIVSPIDSIGAHHFFMHMIQHEII
                     MLISIPLLLLGAPTTPVLRGMPKWLRLGVVAGLAADPIVRMVWRFLTQPLIALIVSTG
                     VLVAWHLIPGWYDAAVRNANIHYIQHLSFAGTAFLFWWNVIDPAPLRASMGYLLRMVY
                     ILVAATAQSVIAAMITLSDRTLYDVYVTARPIYDMSPHSDQELGGLIMWVPGQMVHLI
                     VIGALFAVWAAQSERRQREIEQQQAEAEANASLGAAALHDA"
     gene            1568..3289
                     /locus_tag="PLX85_03470"
     CDS             1568..3289
                     /locus_tag="PLX85_03470"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRC62265.1"
                     /translation="MQTLRVALAQINTTVGDFEGNAAKIVAAAGDAAGHGADLVAFPE
                     LALAGYPPEDLLIRSAFIEDAAGALDSLAARAKGLPPLIVGCTEFDGQLYNAAAIIHA
                     GRVIGTYRKHFLPNYGVFDEYRYFQPGDHAPLFLIAGVEVGITICEDLWFPGGPMREL
                     ALAGARVVVNINASPFHGGKPHFRERMVATRASDNTVAIAWVNQVGGQDELVFDGNSA
                     VFGPDGEVMARAASMDEDLLLVDIPVGQVVQRQLHDVRLRRERRASDRPTPIVVTQQP
                     SPRQRPPLPAYRLQRPDNVAEVYQALVVGTRDYIRKSGFQDAIVALSGGIDSAIVATI
                     AVDALGADHVRGVSLPSRYSSEGSIADAQALAENLGIRLAAMPIEAIHQATLDALARE
                     FRGTEPGTAEENVQSRIRGMLTMALSNKFGSIVLTTGNKSEYACGYATLYGDMAGGFA
                     VIKDVPKTLVYELANYRNSVSEVIPRNSIEKPPSAELRPGQLDTDSLPPYEVLDPIIE
                     AYVEDDMPFEDIVALGFDEATVRRVIGMINRNEYKRRQAAPGVKITPRAFGRDRRLPL
                     ASRYQPY"
     gene            complement(3283..3666)
                     /locus_tag="PLX85_03475"
     CDS             complement(3283..3666)
                     /locus_tag="PLX85_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62266.1"
                     /translation="MTSNRRTHYALRLDAEACGTTLGTLAWVMGALEILKDRGHATGD
                     ERWHELSDQLMPRLERVVQTIAGARMAVAAEAEQEAEVEDAYGRVRDAFERWIEVTAA
                     DTVEAALGFRHQLHDHDGEADAPAQ"
     gene            complement(3663..>4059)
                     /locus_tag="PLX85_03480"
     CDS             complement(3663..>4059)
                     /locus_tag="PLX85_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62267.1"
                     /translation="AVTKEEAKQRWLRGVPEAAALSGSRVVRGAHSRAGALPAFPEVP
                     PVAALPSETVEQAAGLLADAAALVDPEALVLYALPQHLDALVPLTQRVLDEVAPGVRV
                     LRAGLGHQAAVIGALRIAGTIAFEGHRKP"
BASE COUNT          671 a         1410 c         1301 g          677 t
ORIGIN      
        1 tctactggca cttcgtcgac atcgtctgga tcgtgatctt cacggtcgtc tacctcattc
       61 ccacctagcg agcagagcgg gagcacaccg atgagccacg cagaacaggc gtccgaggtc
      121 tccgagcagc acgggctgac cgtccgtcag tacctgatga tcggcgccgc cctcacggtg
      181 atcacgatca tcgagttggc cgtctcttac agtgacctcg gcagcgcgat ggtccccatc
      241 ctcctcgcgc tctccgcggt gaagttcgtg gtggttgtgg cgtactttat gcatctgcgc
      301 ttcgactccg cgctcttcac gcgaatgttc gtgggcagct tcctcctggc actcgcgatt
      361 ctcgtcgcgc tggtcagcct gttctgggcc gacagccaca cgttgccgct gaagtagccg
      421 aaccggccag ggaacgcacc gaagagccgc tggcattgcc agcggctctt cgtctgccgt
      481 gtccgaaccc gaggaatagg ggaggcgcgt taccgatcgg aggccgacta ggcgtcgtgg
      541 agcgcggcgg cacccagcga agcgttcgcc tccgcctccg cctgttgctg ctcaatctcc
      601 cgctgccgcc gctccgactg cgccgcccac accgcgaaca gcgcgccgat gacgatgaga
      661 tgcaccatct ggcccggcac ccacatgatc aggccgccca gttcctggtc ggagtgcggc
      721 gacatgtcgt aaatcggtcg agccgtcaca tacacgtcgt acaacgtgcg gtcggagagc
      781 gtgatcatcg ccgcgatgac ggactgcgcc gtcgccgcga cgaggatgta caccatccgc
      841 aacaggtacc ccatcgaggc gcgcagcggc gccggatcga tcacgttcca ccagaacagg
      901 aacgcggtcc ccgcgaacga cagatgctgg atgtagtgga tgttggcgtt ccgcaccgcg
      961 gcgtcatacc aaccggggat caggtgccac gccacgagga cgcccgtgga cacaatgagt
     1021 gcaatgagcg gctgggtcag gaaccgccac accatccgta cgattgggtc agcggcgagg
     1081 cccgccacga cgccgaggcg cagccatttt ggcatccctc ggaggacggg agtcgtcggc
     1141 gcgccgagca gcagtagcgg gatcgagatc agcatgatga tctcgtgctg aatcatgtgc
     1201 atgaagaagt ggtgcgcgcc gatgctgtcg atcggcgaca cgatggccag gactaggctc
     1261 cccagtccga ggtagtagga cgcgaccttc caccagggat gctgccggtg cctgaccgtc
     1321 cagcgacgca gcccgcgcgc gtagaagaac ccgacgatgc atgctgggag tatgaccgga
     1381 agatcgaggg tccacgcggt ccagaccgaa tggccttcgc gcaccgtatc gtgcgcggcc
     1441 gttcccgtgc tgacgagctg gcccagcagg tcggcgtcca tgtgcacctc cgtgaggcag
     1501 tcgttccagt atgcggcggc tccggggtga ccgatatact ggctgctcgt gggaagcgac
     1561 ggtcgtaatg cagacgctgc gtgtggcgct cgcccagatc aatacgacgg tgggcgattt
     1621 cgagggcaac gccgcgaaga tcgtcgcggc cgccggggat gcggccgggc acggcgctga
     1681 tctcgttgca ttcccggagc tggccctggc cggctacccg cccgaggacc tgctcatccg
     1741 gtcggccttc atcgaggacg ccgccggggc gctcgactcc ctcgccgctc gcgcaaaggg
     1801 actacccccg ctcatcgtcg ggtgcaccga gtttgacggc cagctgtaca acgccgctgc
     1861 catcatccat gcgggccgcg tcatcgggac gtaccgcaag cactttctcc ccaattacgg
     1921 cgtcttcgac gagtaccgtt actttcagcc gggcgaccac gcgcccctct tcctgatcgc
     1981 gggagtcgag gtcggcatca cgatctgcga ggacctctgg ttccccggtg gccccatgcg
     2041 cgagcttgcg ctcgccggcg cgcgtgtggt cgtcaacatc aacgcctccc cgttccacgg
     2101 cggaaagccc catttccgcg agcgaatggt cgccacgcgc gcctcggaca acaccgtcgc
     2161 aatcgcctgg gtgaatcagg tgggagggca ggacgagttg gtcttcgacg gcaactcggc
     2221 ggtcttcggg cccgacggtg aggtcatggc tcgcgccgcc tcgatggacg aggatctcct
     2281 gctcgtcgat atccccgtcg gtcaggtcgt gcaacgccag ctgcacgacg ttcgtctgcg
     2341 ccgcgagcga cgcgcctcgg accggccgac acccatcgtc gtgacgcagc agccatcccc
     2401 gcggcagcgc ccgcccttgc cggcctaccg gctccagcgc ccggataacg tcgccgaggt
     2461 gtaccaggcg ctcgttgtcg ggacccgcga ctacattcgg aagtctggct tccaggacgc
     2521 catcgtcgcg ctctccggcg gcatcgactc ggcgatcgtg gcgaccatcg cggtggatgc
     2581 cctcggcgcc gatcacgtgc ggggcgtctc actgccatcg cgctactcct cggaaggctc
     2641 catcgcggat gcgcaggccc tcgccgagaa ccttggcatt cgtcttgcgg cgatgccgat
     2701 cgaggcgatc caccaggcca ccctcgacgc gctcgctcgg gagtttcgag gcacggaacc
     2761 gggcaccgct gaggagaacg tgcagtcgcg aattcgcggc atgctcacga tggcgctctc
     2821 gaacaagttc ggctcgatcg tgctcaccac cggcaacaaa tccgagtatg cctgcggcta
     2881 cgccaccctc tacggcgata tggcgggcgg cttcgcggtc atcaaggacg tcccgaagac
     2941 cctggtttac gagctggcca actaccgcaa cagcgtgagc gaggtcatcc cgcgcaactc
     3001 gatcgagaaa ccgccctcag ccgaactgcg accgggccaa ctcgacaccg actcgctgcc
     3061 accctacgag gtcctcgacc cgatcatcga ggcctacgtc gaagacgaca tgccgttcga
     3121 ggacatcgtg gcgctcggtt ttgacgaagc gaccgtccgc cgcgtcatcg ggatgatcaa
     3181 ccgcaacgag tacaagcgcc gccaggccgc gccgggcgtc aagatcacgc cacgggcctt
     3241 cggtcgcgat cgacggctgc ccctcgcttc gcgctatcag ccttactgag ccggagcatc
     3301 cgcttcaccg tcgtgatcgt gtagctggtg tcgaaacccc agcgccgcct cgacggtgtc
     3361 tgcggccgtg acctcgatcc agcgctcgaa ggcgtcccgg acgcgcccgt acgcgtcttc
     3421 gacctcagcc tcctgctcgg cttcggcggc gaccgccatg cgcgcgcccg cgatcgtctg
     3481 tacgacccgc tcgaggcgcg gcatcagctg gtcggacagc tcgtgccagc gctcatcgcc
     3541 tgtcgcgtgg ccgcggtcct tgaggatctc gagggcgccc atcacccagg ccagtgtccc
     3601 gagcgtcgtg ccacacgcct cggcgtcgag gcgcagcgcg tagtgcgttc ttcggttgga
     3661 ggtcatggct tccgatgtcc ttcaaaggcg atcgtccccg caatacggag ggcgccgatt
     3721 accgcggcct ggtgacccag tccggcgcgc aggacgcgca ccccgggagc gacctcgtcg
     3781 agcacacgct gagtgagtgg aacgagcgcg tcgaggtgct gggggagtgc gtacagtacg
     3841 agcgcctcgg ggtcgacgag cgccgctgcg tccgccagga gcccggctgc ctgctccacc
     3901 gtttccgagg ggagggctgc gactggtggc acctccggaa acgccggcag cgcgcccgcg
     3961 cgggagtgcg caccacgcac gacccggcta ccggacaggg cggcggcctc cggcacgccg
     4021 cgcagccagc gctgcttcgc ctcttccttc gtcaccgcg
//
LOCUS       DAORPG010000108         4000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1766_length_4000_cov_3.365019, whole genome shotgun sequence.
ACCESSION   DAORPG010000108 DAORPG010000000
VERSION     DAORPG010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1766_length_4000_cov_3.365019"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PLX85_03485"
     CDS             complement(<1..501)
                     /locus_tag="PLX85_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRC62268.1"
                     /translation="MTQDAANAPQMPTRRFRHLLLGVVRTQRLRVLEAFVCAILTVEF
                     VIGLQKGQQILIDEAFPGANHPGDVDVLAYVMGGLAVGLIAASLAALRQNYLTAYLSG
                     RAARDLRVRMFERLQYLRYGSFERLPHGDISARLTADLAVVEWALATVLGQGMQLALT
                     LVAATAT"
     gene            complement(723..1673)
                     /locus_tag="PLX85_03490"
     CDS             complement(723..1673)
                     /locus_tag="PLX85_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62269.1"
                     /translation="MAYEVAYTEATSGGGAALYLADGEAREVRRVSGEDGVARQPAWS
                     PDGQQVAYAGCGRPEQERCSLYRADIDGGTPKGLGEGAWPQWSPDGQRLAFVKARGAG
                     DELFVTDSMRDAPRLKTAGSDTDAGGGGVMQAGVSSWSPDGRELAVTRRLEGGEGSEV
                     LLVRADGLRELRVGGDLEAATFAGWSPDGTSMLLFGREHGAGGGLFLMAGDGTGMRRL
                     ASVDAADAARAAWSPNGRQVAMTWGDGLAVVDVADGTTRQLADGECLGAGGLEWGSDA
                     SIWFTRGCDARGRALVELDVASRAERTFSASVIEFALRPR"
     gene            complement(1754..3391)
                     /locus_tag="PLX85_03495"
     CDS             complement(1754..3391)
                     /locus_tag="PLX85_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62270.1"
                     /translation="MLTSKAGSEAVEALRRPLHAGVLARSEVVVAVALVAGLVVRLYY
                     LVPAGGFPLNDGGLFYSMARDLQAHDFITPARTSYNLDAIPYAYPPLMVYAIALLDRF
                     TPIDLITSLRFLPALASLATIPVFYALARKLLPGTSASAAAIAFAVLVEGFHWHIMGG
                     GITRAFGLLFALLAIQQAVRLFIERDGRGLLPVIGWASAAQLTHPNLVAPIAIGLIVL
                     LHGRSRPALKQAAIVAGSVLVLTAWWWGALVLRDGAGTFLALRASRFSEYFYHDGYPW
                     RALLDWSFTTQPVLNWPAALALIGAAALIARGAYLVPLWLVAEFVLEPTQAHNFLPAP
                     VALLVGAGVGLVVLPALTRPWAFEEPEAPAAWRRWARPRWGLGSRIATTGFVTVLGVI
                     ALSSALAKPELPALRSLSGDERANLEWIARETPADARFLVVSGQPWWADGLSEWFPAL
                     TGRHSDYTAQGYEWLGVEFPNRIYWHDRLQGCGGEDASCLDDVVVRSQSSYDYVYVRT
                     NCCGELRDAILSSTRFRVVRQNEAGIIAVVVRQALAS"
     gene            complement(3541..>4000)
                     /locus_tag="PLX85_03500"
     CDS             complement(3541..>4000)
                     /locus_tag="PLX85_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HRC62271.1"
                     /translation="FAAVLFVAAARSGGGGSAKDDGVGSAGEPAPSVTSTKEATTPSR
                     TPTGTPTAAGTRTPTSTPAPPNAATLAVQARQSANVRVTVDGRIDYDGALRAGETRTW
                     AGSSNVELWTDNGKNVSVMVNGFDLGALSTAVGHPEWNTVDWSWAAGWHP"
BASE COUNT          637 a         1559 c         1233 g          571 t
ORIGIN      
        1 cgtcgccgtc gccgccacga gtgtgagcgc cagctgcatc ccctgcccca gcaccgttgc
       61 cagcgcccac tcgacgaccg caaggtcagc ggtaagacgc gccgaaatat cgccgtgcgg
      121 gagacgttcg aaactgccgt agcgcaggta ctgcaagcgc tcgaacatcc gcacccggag
      181 gtcgcgcgcg gcccgtccgg acaggtacgc cgtgaggtag ttctgccgga gcgcggcgag
      241 agaggcggca atcagaccga ccgccaggcc tcccattacg tatgccagca cgtccacgtc
      301 tcccgggtga ttcgctcccg gaaacgcctc atcgatcaga atctgctgcc ccttttggag
      361 gccaataacg aattcgacgg taagaatggc acacacgaat gcctcgagta cgcgcaggcg
      421 ctgggtgcgc accacgccca gcagcaggtg ccgaaaacgc cttgtcggca tctggggcgc
      481 gttcgctgcg tcctgggtca tggcccgcct cggtggcttt ccatgtgcat tcggagtgtg
      541 cggcggttca gacggtcctg gatcatcgac acactcgacc gctggtgacc tggagccgcc
      601 ggacgaggat agcactggcc tctgattcgc cggccggtgg tatccacggt cgcggccccg
      661 gcgcgcggtg gccaaccacg cggccctcgc gtcgtccgcg ccgaggtggc tgggctcgat
      721 gactatcgcg gccgcagcgc gaactcgatc acggacgccg agaacgtccg ctccgctcgg
      781 ctcgccacgt ccagctccac gagcgctcgc ccgcgggcat cgcagccgcg tgtgaaccag
      841 atcgaggcgt cactgcccca ctcgaggccg ccggcgccga ggcactctcc gtcggcgagc
      901 tgccgtgtcg tgccgtcggc cacgtccacc acagccagcc catcccccca ggtcatggcg
      961 acctgtcgcc cattgggcga ccacgctgcc cgtgccgcat cggccgcatc aacgctcgcc
     1021 agccgccgca tcccggtccc gtcgcccgcc atgaggaaca gcccgccgcc ggccccatgc
     1081 tcgcgaccga acagcagcat cgacgtgcca tccggcgacc acccggcgaa cgtcgccgcc
     1141 tcgaggtcgc ctccgacgcg cagctcccgg agcccatcgg cccgcacaag gagcacctcg
     1201 ctgccctccc cgccctcgag acgccgggtc acggccagct cccggccgtc cggggaccag
     1261 ctgctgacac ccgcctgcat cacgccgccg ccgcccgcat cggtgtcaga ccccgccgtc
     1321 ttcagccgcg gagcatcccg catgctgtcg gtgacaaaca gctcgtcccc ggcgccgcgc
     1381 gctttcacga acgcgagccg ctggccgtcc ggtgaccact gcggccacgc tccctcaccg
     1441 agtcccttcg gtgttccgcc gtcgatgtcc gccctgtaca gcgagcaccg ctcctgctcc
     1501 ggcctcccac atcccgcgta cgccacctgc tgtccgtcgg gtgaccatgc cggctgccgc
     1561 gcgacgccgt cctcaccact gacccgccgg acctccctgg cctcgccatc cgcgaggtac
     1621 agcgctgccc ctcccccgct cgtcgcctcg gtataggcga cctcgtacgc cacacggggc
     1681 gcgtgcgccc cggaaccgca cgccgcggcc acgagtgaga gtccagtcag gacagccgcg
     1741 gccaaccccc gagtcacgac gccagcgcct gcctcactac cactgcaatg atccctgcct
     1801 cgttctgtcg cacgactcgg aagcgcgtcg agctcagaat ggcatcgcgc agctccccgc
     1861 agcagttggt ccgtacgtag acgtagtcgt aggagctctg cgatcgcacg accacgtcat
     1921 cgaggcagct tgcgtcttca ccgccgcatc cctggagccg gtcgtgccag tagatgcggt
     1981 ttgggaactc cacacccagc cactcatacc cttgcgccgt gtagtcgctg tgccggcccg
     2041 tcagcgccgg gaaccattcc gacagcccgt ctgcccacca cggctgcccc gacacgacga
     2101 ggaagcgagc gtccgccgga gtctcccgcg cgatccattc gaggttcgcc cgctcgtcgc
     2161 cgctcaggct gcgcaacgcc ggcagctcag gcttcgctag tgccgaggac agcgcgatca
     2221 ccccgagcac cgtcacgaat ccggtcgtcg cgatcctcga cccgagcccc catctgggcc
     2281 gtgcccatcg tcgccaggcc gccggcgcct ccggttcctc gaacgcccag gggcgtgtca
     2341 aagccgggag caccacgagc ccgacgccgg cccccacgag caacgccacg ggtgccggca
     2401 gaaaattgtg tgcctgcgtc ggctccaaga cgaactcggc caccagccac agcgggacga
     2461 ggtacgcgcc ccgcgcgatc aacgccgccg ccccgatcag cgccagcgcg gcgggccagt
     2521 tcagcaccgg ctgcgtcgta aacgaccagt ccagcagcgc ccgccacggg taaccatcgt
     2581 ggtagaagta ctccgagaac cggctcgccc ggagtgcgag gaacgtgccc gcgccgtccc
     2641 gcaggacgag cgccccccac caccacgccg tcaacaccag cacgcttccc gccacgatcg
     2701 ccgcctgctt gagcgcgggc cgggaccgtc cgtgcagcag cacgatgagc ccgatggcga
     2761 tcggcgcgac gaggttcggg tgggtcagct gagcggcgct cgcccagccg atcaccggca
     2821 gcaaccctcg cccgtcacgt tcgatgaaca ggcggaccgc ctgctgaatc gcgaggagcg
     2881 cgaagagcag cccgaacgcc cgcgtgatgc ccccacccat gatgtgccag tggaagcctt
     2941 ccaccagcac cgcgaaggcg atcgccgccg cgctcgccga ggtcccgggc aacaacttcc
     3001 gtgcgagcgc atagaacacc gggatcgtcg ccaggctcgc gagcgccggc aggaagcgca
     3061 ggctcgtaat caggtcgatc ggtgtgaacc ggtcgagcag tgcgatcgcg tacaccatca
     3121 acggcgggta cgcgtacggg atcgcgtcga ggttgtacga ggtcctcgcg ggtgtgatga
     3181 agtcatgcgc ctgcaggtcg cgggccatcg agtagaacag gccgccatca ttcagcggga
     3241 atccccccgc cggcacgagg tagtacaggc gtacgaccag tcccgccacc agcgcgaccg
     3301 cgacgaccac ttccgagcgc gcgagcacgc ctgcatgcag tggccggcgc agcgcctcga
     3361 cggcctcgct gccggccttc gatgtcagca tcctgccctt actcctggcc aactctcgcc
     3421 gcgtgcggcg gcaacccgtc cggtcgtgcg cgcggcccgc tcattagcat ggcccgtgac
     3481 gcaagccaac gctgcgccat gctccctcgc ggtcgcccgt gctggcgcgt gcggtcgagg
     3541 tcagggatgc cagcccgctg cccagctcca gtcaaccgta ttccactccg ggtgccccac
     3601 tgccgtcgac agcgcgccga ggtcaaagcc gttcaccatc accgacacgt tcttcccatt
     3661 gtccgtccag agctcgacat tgctgctccc cgcccacgtt cgcgtctccc ccgcacgcaa
     3721 cgctccgtcg tagtcgatgc gcccgtccac cgtcacccgc acgttcgcgg actgccgagc
     3781 ctgtaccgcg agcgtcgcgg cgtttggagg cgcgggcgtc gaggttggcg tgcgtgtacc
     3841 cgcggcggtg ggggtccccg tcggagtgcg cgacggcgtc gtcgcctctt tcgtggacgt
     3901 caccgatggc gcaggctcgc ccgccgagcc gaccccatcg tcctttgccg atccgccgcc
     3961 cccggagcgc gcggccgcaa cgaacaggac tgctgcgaac
//
LOCUS       DAORPG010000109         3978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1786_length_3978_cov_3.570227, whole genome shotgun sequence.
ACCESSION   DAORPG010000109 DAORPG010000000
VERSION     DAORPG010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1786_length_3978_cov_3.570227"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /gene="cofD"
                     /locus_tag="PLX85_03505"
     CDS             complement(<1..747)
                     /gene="cofD"
                     /locus_tag="PLX85_03505"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HRC62272.1"
                     /translation="MRITVLAGGVGAARFLDGLIRVTDPAEVTVICNVSDDFAWHGLF
                     VCPDIDTVVYTLAGLEGDQGWGVRGDTRAALDELTALGEDPWFLIGDRDLATHVWRTE
                     RLRTGHPLSEVTRDLAAARGLACSLLPVTDDPHPTVVLTEDGPLGFQDYFVRRRASDA
                     VRGFRFPGAHAAQPAPGVLDALTTADVVVFAPSNPFVSIAPILEVPGVREALAATAAR
                     RIAVTPIVGGAAIKGPAASMLASLGHAVSAL"
     gene            886..1605
                     /locus_tag="PLX85_03510"
     CDS             886..1605
                     /locus_tag="PLX85_03510"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HRC62273.1"
                     /translation="MSTLSEDIERRLREQAGLYVIVEEQDGALVLSGLVDTEDDRAAA
                     LDVVGEMAPGYEVDDDIEVSAGIPEQTTAGRLMEGGAGMFADAEADLDMEAEAIEPGD
                     FTDQPIEEYGDYAAGPSSSFEEDEASDGDEVFVPPTDPVGTDREVIGGLSTTSMDEVD
                     VEPSSDGSLGDEAIRDAILRELREDAMTTDLQIEVTVLEGLVTLTGTVPSIDSAESAE
                     EVAARVPGVVEVDEELDVEEL"
     gene            1589..2176
                     /locus_tag="PLX85_03515"
     CDS             1589..2176
                     /locus_tag="PLX85_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HRC62274.1"
                     /translation="MSRSSDGLRLWTVGHSTRSFEELCAVLESPEVGPIEVLADIRTA
                     PRSRRHPHFNLEALAEELPRRGLEYVHLKRLGGFRRPRPDSQNGAWRNESFRGYADYL
                     ETAEFASALDELFAIARRARTAVMCSEGVPWRCHRSLLADALRARGVDVTHLLSATKA
                     QGHALTSFAVVEGERVTYPAAEEPVEGEADVSAAV"
     gene            2157..2456
                     /locus_tag="PLX85_03520"
     CDS             2157..2456
                     /locus_tag="PLX85_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62275.1"
                     /translation="MFQRQYEDDADVVTESGELVARGYARLVLERSDEGASWRGTFRV
                     TEPVEPPHLEGPQRLRLRGGAQGEAEFVPTEDVQGEVAGRAGTFFEVTGAGPAPF"
     gene            complement(2475..>3978)
                     /locus_tag="PLX85_03525"
     CDS             complement(2475..>3978)
                     /locus_tag="PLX85_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62276.1"
                     /translation="LPRAERSVSRPRLGGASIALAICGLLLSAVYAVATARIPLARYF
                     PEAGITVDFVKMLGPGWERPTIEYVDVVLITFALYLGALAAVWRSHTDIPRWLLFGMP
                     AAFALILLSMYPPTAVDMFHYQASSRVLWVHGENPLTVAPGNFPYPIGYSWAGQPAPY
                     GPLWPILAAPAVLFTGDHYLLGLYGFKLIAAASYLGCAVMIWRIIAHTRPGEETLAVV
                     LFAWNPFVVLRVVGNGHNDLVMMLFLLLALDLALRRHWNYAFVAFACSALVKYVSLLL
                     GPPLLIYLLAHAEGTPRQKLGVAAQALGLAALTVIVVFAPLWDGLATFDTVRGEADKV
                     ITSTPLLIGLLLSGPPEDPQTVERARTVLRLVFLCLYFPLVWQARRSFDHLAVASFTI
                     LFLYILVPTGWFRPWYMLWPVTLAALRPRSWLAAVLLAITFACCFPDLIEQYRNNWTF
                     LAEYKKAVAAPIVVAFGPPALVWLAGVAWKRGWMLGASRAPIEASGRAST"
BASE COUNT          664 a         1121 c         1689 g          504 t
ORIGIN      
        1 gagggcggag accgcgtggc cgagggaggc gagcatcgag gccgcggggc ccttgatggc
       61 ggcgccgccg acgatggggg tgaccgcgat gcggcgggcg gccgtggccg cgagtgcttc
      121 gcggacgccg ggcacctcga ggatgggggc gatggagacg aaggggttgc tgggagcgaa
      181 gacgacgaca tccgcggtcg tgagggcgtc gaggacacca ggcgcgggct gcgcggcgtg
      241 cgcgccgggg aagcgaaagc cgcggacggc atcggaggcg cggcggcgga cgaagtagtc
      301 ctggaagccg agcgggccgt cctcggtgag gacgacggtc gggtgggggt cgtccgtgac
      361 ggggagcagc gagcaggcga ggccgcgggc cgcggcgagg tcccgggtga cctcgctcag
      421 ggggtggccg gtgcggaggc gctcggtgcg ccagacgtgc gtggccaggt cgcggtcgcc
      481 aatcaggaac caggggtcct cgccgagtgc ggtcagctcg tcgagggcgg cgcgcgtgtc
      541 gcctcggacg ccccatccct ggtcgccctc gaggccggcg agtgtgtaga cgaccgtgtc
      601 gatgtcaggg cagacgaaca ggccgtgcca ggcgaagtca tcgctgacgt tgcagatgac
      661 ggtgacctcg gcggggtcgg tcacgcggat gaggccatcg aggaagcggg cggccccgac
      721 gccgccggcg agcacggtga tacgcatggc ggcgatcata gcgggctttg acctcggaac
      781 gggaaccgcg gggcgagcgg cctttggccc ctcgtgacgg gacgaagggc cctcggcggc
      841 ctcgatacag tggatccaga ggcctgaagg gtggaggagg taggcgtgag cacgttgagc
      901 gaggacatcg agcggcggtt gcgggaacag gcggggctgt acgtgatcgt ggaggagcag
      961 gacggcgcgc tcgtgctgag cggactcgtc gacacggagg atgaccgtgc agctgccctc
     1021 gatgtcgtgg gggagatggc cccgggctat gaggtcgacg acgacatcga ggtgagcgcc
     1081 gggattccgg agcagacgac tgcggggcgg ctgatggaag gcggcgccgg gatgttcgcg
     1141 gacgcggagg ccgacctcga tatggaggcg gaggcgatcg agccgggcga cttcaccgac
     1201 caaccgatcg aggagtacgg ggactacgcg gcgggccctt cgtcctcgtt cgaggaggac
     1261 gaggcgtcgg atggcgacga ggtgttcgtg ccgcccacgg acccggtggg caccgaccgc
     1321 gaggtcatcg gtgggctgtc gacgacctcg atggatgagg tcgacgtcga gccgtcctcg
     1381 gacgggtcgc tgggcgacga agcgatccgc gacgcgatcc tgcgcgagct gcgcgaggac
     1441 gcgatgacga cggacctgca gatcgaagtg accgtgctcg aagggctggt gacgctcaca
     1501 ggcacggtgc cctcgatcga ctcggcggag agcgccgagg aggttgccgc gcgggtgccg
     1561 ggcgtggtgg aggtggacga ggagctggat gtcgaggagc tctgacgggc tgcgcctctg
     1621 gaccgtcggg cattcgacgc ggagcttcga ggagctctgc gcggtcctcg agagccccga
     1681 ggtggggccg atcgaagtgc tggcggacat ccggacggcg ccgcggtcgc gacgacatcc
     1741 gcacttcaac ctcgaggcgc tggccgagga gctgccgcga cgcgggctgg agtacgtgca
     1801 cctgaagcgc ctcggcggat tccggcggcc gcgccccgat tcgcagaacg gtgcctggcg
     1861 gaacgagagc ttccgcggct atgccgatta cctcgagacg gccgagttcg cgtcggcgct
     1921 cgacgagttg tttgcgatcg cccggcgtgc gcggacggcc gtgatgtgct cggaaggggt
     1981 gccgtggcga tgccatcgct cgctacttgc ggacgcgctc agggcgcgtg gcgtcgacgt
     2041 gacgcacctg ttgtcggcga cgaaggcgca ggggcacgcg ctgacgtcgt tcgcggtggt
     2101 cgagggggag cgggtgacgt acccggcggc ggaggaacca gtcgaggggg aggccgatgt
     2161 ttcagcggca gtatgaggac gacgcggatg tcgtgacgga gtcgggcgaa ctcgtcgcgc
     2221 ggggctacgc acgactcgtg ctcgagcgta gcgacgaggg cgcgagctgg cgcgggacgt
     2281 ttcgggtcac ggaacccgtc gagccgccgc acctcgaggg gccgcaacgg ctgcggttgc
     2341 ggggtggcgc gcagggcgag gcggagttcg tgccgaccga ggacgtgcag ggcgaggtag
     2401 cgggacgggc ggggacgttc ttcgaggtga cgggggcggg gccggcgccg ttctgacgtg
     2461 ctcgcctcgg cgggtcaggt cgaggctcgg ccgctcgcct cgatcggtgc ccggctcgcg
     2521 ccgagcatcc agccgcgctt ccaggcaaca cccgcgagcc agaccagggc gggcggcccg
     2581 aaggcgacga cgatcggagc ggcgacggcc ttcttgtact cggcgaggaa cgtccagttg
     2641 ttgcggtact gctcgatgag gtcggggaag cagcaggcga aggtgatggc gaggaggacc
     2701 gcggccagcc agctcctcgg gcggagggcg gcgagggtga ccggccagag catgtaccag
     2761 gggcggaacc agccggtggg aaccaggatg tagaggaaga ggatcgtgaa gctcgcgacg
     2821 gcgaggtgat cgaaactgcg gcgggcctgc cagaccaagg ggaagtagag acagaggaag
     2881 acgaggcgca gcaccgtgcg ggcgcgctcg acggtctgcg ggtcctcggg ggggccggag
     2941 agcaggaggc cgatgagcag gggcgtcgag gtgatgacct tgtccgcctc acctcggacg
     3001 gtgtcgaagg tggcgaggcc gtcccagagc ggggcgaaga cgacgatcac ggtcagtgcg
     3061 gcgaggccga gggcctgggc ggcgacaccg agcttctgac gcggcgtgcc ctcggcgtgc
     3121 gcgagcaggt agatgaggag cggcggaccg aggagcaggc tgacgtactt cacgagggcg
     3181 gagcaggcga aggcgacgaa ggcgtagttc cagtggcggc ggagggccag atcgagggcc
     3241 agcaagagga acagcatcat gacgaggtcg ttgtggccgt tgcccacgac gcggaggacg
     3301 acgaacgggt tccaggcgaa gaggacgacc gcgagcgtct cctcgccagg gcgggtgtgg
     3361 gcaatgatgc gccagatcat gacggcgcag ccgaggtagc tcgcggcggc gatcagcttg
     3421 aagccgtaga ggccgagcag gtagtggtcg ccggtgaaga gcacggcggg ggcggcgagg
     3481 atcggccaga gcggaccgta cggggcaggc tggccggccc agctgtagcc gatggggtag
     3541 ggaaagttcc cgggggcgac ggtcagcggg ttctcgccgt gcacccagag cacgcgtgac
     3601 gaggcctggt aatggaacat gtcgaccgct gtcggcgggt acatcgagag gaggatcagg
     3661 gcgaaggcgg ccggcatgcc gaagaggagc cagcggggga tgtccgtgtg cgaccgccag
     3721 acggccgcga gcgcgccgag gtagagggca aaggtgatga gcacgacgtc gacgtactcg
     3781 atggtggggc gctcccagcc gggcccgagc atcttgacga agtcgacggt gatgccggcc
     3841 tcggggaagt agcgcgcgag ggggatgcgc gccgttgcga cggcgtagac ggcggaaagc
     3901 agcaggccac agattgcgag ggcgatggag gccccgccga ggcggggccg actaacgctg
     3961 cgttcggccc gcgggagg
//
LOCUS       DAORPG010000110         3952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1812_length_3952_cov_3.796510, whole genome shotgun sequence.
ACCESSION   DAORPG010000110 DAORPG010000000
VERSION     DAORPG010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1812_length_3952_cov_3.796510"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PLX85_03530"
     CDS             <1..800
                     /locus_tag="PLX85_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62277.1"
                     /translation="AGGGGEEVAPASTTAAPPTPAPTAELALPAGPRQPNDLVYVAVN
                     DPPQDQLVLVQYAAGLARDFFFRQLGFYLDGPLTINVANLPALELHGATFLREGDRIS
                     TVTVNVGQATWEMEAPFERAKVVAHEYFHVLQNWLRGSTDTAADPLFLVEGAAEYAGY
                     ATVIDAGLTSWNDLRHGLEERLRNDRVTLPALDRLSDDGPLALSGYQLSALAIDRLVG
                     AAGVQPLARYALLRSREQPPTAFELVFRRSLPDFYAEIAGWRQSSGL"
     gene            complement(876..2276)
                     /gene="fumC"
                     /locus_tag="PLX85_03535"
     CDS             complement(876..2276)
                     /gene="fumC"
                     /locus_tag="PLX85_03535"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HRC62278.1"
                     /translation="MTTTSASTRTETDSFGPIEVDSSVYWGAQTQRSLQNFKIGGERF
                     PRPLIRALAIIKHASATVNQDLGKLDATRADAIREAAQEVIDGKLDDHFPLVVWQTGS
                     GTQTNMNANEVISNRAIEILGGALGSKDPVHPNDHVNMSQSSNDVFPGAMAVATAEQV
                     VHLTLPGLRYLRDALQAKSTEFDSIVKIGRTHLMDATPLTLGQEFSGYVQQVSYGIAR
                     IEGVLPRLYELALGGTAVGTGLNTHPEYAERVAAEISRLTGLPFVTAPNKFESLAAHD
                     AQVELSGQLKTVAASLMKIANDIRFLGSGPRAGIAELMLPENEPGSSIMPGKVNPTQS
                     EALTMVCAQVMGNDVAVNIGGATGHFELNVFKPVIVYNTLQSLRLIGDASRSFTDNMV
                     AGIKPNMSRIEDLMRSSLMLVTALSPHIGYDKAAKVAHQAHHEGTTLKEAALALGYVT
                     SEQFDEWVRPEDMIHP"
     gene            complement(2295..3230)
                     /gene="mdh"
                     /locus_tag="PLX85_03540"
     CDS             complement(2295..3230)
                     /gene="mdh"
                     /locus_tag="PLX85_03540"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRC62279.1"
                     /translation="MRKKVTFVGAGMTGSAMAQMMAARGYADVVVIDIVDGLPQGKAL
                     DIAESAPWVGFSTSIVGTNDWTRTANSDVVVVTSGVPRKPGMTREDLLGTNANIVRSV
                     VKNAVQHSPDAVFIIFANPMDAMCHVALEASGLPKERVIGQGGMLDSSRYRTFIAMET
                     GMSVRDVSAWVLGGHTEATMVPIVSNATVGGVPLTQLLAPKKVQAVVERTKRGGAEIV
                     ELLKTGSAFIAPAAATIEMVDAVLLDQRRVLPCATLLTGQWGVRNGYVGAPVLVGEGG
                     IQKVYAPPLADDEVAAIKAAGVAVRDLVKATPPAA"
     gene            complement(3327..>3952)
                     /locus_tag="PLX85_03545"
     CDS             complement(3327..>3952)
                     /locus_tag="PLX85_03545"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006726112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRC62280.1"
                     /translation="GLARGWPVYLSTKNTILKAYDGRFKDLFQEVFDAEFKDRFNEAG
                     ISYEHRLIDDMVAQALKSSGRFVWAAKNYDGDVQSDVVAQGFGSLGLMTSVLLTPDGQ
                     TVEAEAAHGTVTRHYREHQKGNPTSTNPIASIFAWTRGLNHRANLDGNPDLEFFASVL
                     ERVCIETVEAGFMTKDLAILIGPEQPWLTTQEFLAKLDERLQAAISA"
BASE COUNT          594 a         1381 c         1283 g          694 t
ORIGIN      
        1 aggccggcgg tggcggcgag gaggtggctc cagcgagcac gaccgccgcg cctccgacgc
       61 cggcgcccac ggcggagctg gcattgccgg ccggcccccg acagccaaac gacctcgtgt
      121 acgtggccgt gaatgaccca ccccaggatc aattggtcct cgtgcagtac gcggccggcc
      181 tcgcgcgtga cttcttcttt cgccagctcg gcttctatct cgatgggccg ctcactatca
      241 acgtcgccaa cctcccggcg cttgagctgc acggcgccac cttcctccgt gaaggagacc
      301 gtatcagcac ggtcaccgtg aacgttgggc aggcgacctg ggagatggaa gcgccttttg
      361 agcgagccaa ggtcgtcgct cacgagtact tccacgtcct gcagaactgg ctgcgcggca
      421 gcaccgacac cgccgccgac cctctcttcc tcgtcgaagg agccgctgag tacgccggct
      481 atgccaccgt catcgacgct ggcctgacca gctggaacga cctgcgccac ggcctggagg
      541 aacgtctgcg taacgaccgc gtcacgctcc ccgcgctcga ccgcctgagc gacgacgggc
      601 cgctcgcgtt gagtggctac caactatctg cgcttgcgat cgaccgcctc gtcggtgccg
      661 ccggcgtgca gccgctggcg aggtacgcac tcctgcgatc gcgggagcag cctcccactg
      721 cgttcgagct ggtgtttcgc cgcagcctgc cggacttcta tgcggagatc gccggctggc
      781 ggcagagcag cgggctctga cagaggggga cagaccgaac agcggcgccc atgggcgccg
      841 ctgttgccgt tcaacgagtc ccccgccagg agcgcttagg ggtgaatcat gtcctcggga
      901 cgcacccact cgtcgaactg ttcggaagtg acataaccga gggccaatgc cgcttccttt
      961 agggttgtgc cttcgtggtg cgcctggtgg gccaccttgg ccgccttatc gtagccgatg
     1021 tgcgggctga gtgcagtgac cagcatcagc gaggaccgca tcagatcctc gatgcggctc
     1081 atgttaggtt tgatgcccgc caccatgttg tcggtgaacg agcgggaggc gtccccgatg
     1141 agccgaagac tctgcagcgt gttgtagacg atcacgggct tgaagacgtt cagctcaaag
     1201 tggccggtgg cgccgccgat gttcacggcg acgtcgttcc ccatcacctg cgcgcagacc
     1261 atggtcagcg cctcggactg cgtggggttc accttgcccg gcatgatcga ggagcctggc
     1321 tcgttctccg gcagcatcag ctcggcgatg ccggcacgcg gcccggagcc gaggaagcgg
     1381 atgtcgttcg cgatcttcat gagcgaggcc gcgaccgtct tcagctgccc ggacagctcg
     1441 acctgcgcat cgtgcgccgc gagcgactcg aacttgttcg gcgccgtaac gaacggcagg
     1501 cccgtgagcc ggctgatctc cgccgccacg cgctccgcgt actcgggatg cgtattgagt
     1561 cctgtcccca cggcggtacc gccgagggcc agctcgtaga gccgaggcag cacgccctcg
     1621 atgcgcgcga tgccgtagct cacctgctgc acataccccg agaactcttg cccgagcgtg
     1681 aggggcgtcg cgtccatcag gtgcgtgcgg ccgatcttca cgatcgagtc gaactccgtg
     1741 gacttcgcct gtagcgcgtc gcggaggtag cgcagccccg gcagggtcag gtgcacgacc
     1801 tgctccgccg tcgcgaccgc cattgccccg gggaacacat cgttcgagga ctgcgacatg
     1861 ttcacgtggt cgttcgggtg caccgggtcc ttggacccga gggcgccgcc gaggatctcg
     1921 atcgcacggt tcgagatcac ctcgttggcg ttcatgttcg tttgtgtccc ggagcccgtc
     1981 tgccagacga cgagcgggaa gtggtcgtcg agcttgccgt cgatcacttc ctgcgccgcc
     2041 tcgcggattg catccgcacg ggtcgcgtcg agcttgccga ggtcctggtt caccgtcgcc
     2101 gaggcgtgct tgatgatcgc gagcgcccgg atcagcgggc gcgggaaccg ctcgccgccg
     2161 atcttgaagt tctggagcga gcgctgggtc tgcgctcccc agtacacgct cgagtcgacc
     2221 tcgatcggcc caaagctgtc ggtctccgtg cgcgtgctcg cgctggtcgt tgtcatcctg
     2281 tccctcgttc cggactaggc cgccggcggc gtggccttca ccaggtcgcg caccgcgaca
     2341 ccggccgcct tgatcgccgc gacttcgtcg tcggccagcg gcggcgcgta caccttctgg
     2401 atgccgcctt cacccacgag gaccggcgcg ccgacgtagc cgttgcgcac gccccactgg
     2461 cccgtgagca gcgtcgcgca cggcaggaca cgccgctggt cgagcagcac cgcgtcgacc
     2521 atctcgatgg tggccgcggc cggcgcgatg aacgcgctgc cggtcttcag cagctccacg
     2581 atctctgcgc cgccgcgctt cgtgcgctcg acgacggcct gcaccttctt cggcgccagg
     2641 agctgggtca gcggcacgcc gcccacggtc gcgttcgaaa cgatcgggac catcgttgcc
     2701 tcggtgtgtc cgccgaggac ccacgcgctc acgtcgcgca ccgacatccc ggtctccatc
     2761 gcgatgaagg tccggtagcg cgaactgtcg agcatgccgc cctggccgat gacgcgctcc
     2821 ttcggcagtc cgctcgcctc gagggccacg tggcacatcg cgtccatggg gttcgcgaag
     2881 atgatgaaca ccgcgtcggg ggagtgctgt acggcgttct tcacgacgct gcgcacgatg
     2941 ttggcgttcg tccccagcag gtcctcgcgc gtcatgcccg gcttgcgcgg cacgcccgag
     3001 gtcacgacga cgacgtccga gttcgccgta cgggtccagt cgttcgtgcc cacgatcgag
     3061 gttgagaacc cgacccacgg cgccgactcg gcgatgtcca gcgccttacc ctgtggcagc
     3121 ccgtcgacga tgtcgatcac gacgacgtcg gcgtagccac gggccgccat catctgggcc
     3181 atcgcgctgc cggtcatccc ggcgcccaca aacgtcacct tcttacgcag agcgcatctc
     3241 ctccacttcg tcccgaagga cgcccttcgc tcgggcggcg cccggcgatg tcatgccgaa
     3301 tcacaaccgg ccgcgcgagg gacgcgctag gccgaaatcg ctgcctgtag ccgctcgtcg
     3361 agcttcgcga ggaactcctg cgtcgtcagc cacggctgct ccgggccgat caggatcgcc
     3421 aggtccttcg tcatgaatcc cgcttcgacc gtctcgatgc agacgcgctc caggacgctc
     3481 gcgaagaact cgaggtcggg gttgccgtcg aggttcgcgc gatggttgag cccgcgcgtc
     3541 caggcgaaga tcgaggcgat cgggttcgtg gaggtagggt tgcccttctg gtgctcccgg
     3601 tagtgccgcg tgaccgtgcc atgcgccgcc tcggcctcga cggtctgccc gtccggcgtc
     3661 agcaacaccg aggtcatgag ccccagcgat ccgaagccct gcgccacgac gtccgactgc
     3721 acgtcgccgt cgtagttctt ggcggcccac acgaagcgcc cgctcgactt gagggcctgc
     3781 gccaccatgt cgtcgatcag ccggtgctcg taggagatgc ccgcctcgtt gaagcggtcc
     3841 ttgaactcgg cgtcgaacac ctcctggaag aggtccttga aacgcccgtc gtaggccttg
     3901 aggatggtgt tcttcgtcga caggtacacc ggccagccgc gcgcgagccc gt
//
LOCUS       DAORPG010000111         3951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1813_length_3951_cov_2.352669, whole genome shotgun sequence.
ACCESSION   DAORPG010000111 DAORPG010000000
VERSION     DAORPG010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1813_length_3951_cov_2.352669"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PLX85_03550"
     CDS             complement(<1..775)
                     /locus_tag="PLX85_03550"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC62281.1"
                     /translation="MDWSDSPQQAEFRASARSFISERLPDYYRGRSRVHGGEDDWQQD
                     FANGTDEAKAAAKRWAEALAERGWGAPQWPKEYGGGGLSALEQYILAEEMAQQNAPVI
                     GGAGVTHIGPTLLVHGTDEQRKQFMGPTLRGEILWAQGFSEPGAGSDLGSLQCRAVRD
                     GDEYVINGQKLWTSAGHKANWIFGLFRTDPEAPKHRGISFLLLDARTEGVSVRPIISM
                     AYEHATNETFYENVRVPAGQLVGEENRGWYVGMTLLDFER"
     gene            937..2223
                     /locus_tag="PLX85_03555"
     CDS             937..2223
                     /locus_tag="PLX85_03555"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRC62282.1"
                     /translation="MLESRRRRSTKAAPVAPAEDVRSRGLMARLERTLAERVGVPRRI
                     LRQAGVVADVTIALALFADAMVALATSSPGVEFGTREPDAFAYLLGAVMTLPLAVRRR
                     WPVEVLGVALAGHIAFNLRDYAGVNVDFFGPIVAMYTVAAHRPRRVSVPAAAVVLLGV
                     FIADPPFDEPLGNFVATVLIIAGIWLFGDAARVRRIYGERLAAQAEALREARMELAEQ
                     AVTQERLRIARELHDVVAHHMSAIVVQSALAQDRLEVDPPAARRAIGQVESMGRGALR
                     EMRQILGVLRQAGEDRGALDPSPSLGDLERLYARARDGGLNVSAAIEGDLSGLPPALE
                     LAAYRIVQESLTNALKYAPGSRVDVHLARDAHQVTVEVVDDGPAPSSPPTTAGGGQGL
                     IGMRERVALLAGNFEAGRTPSGGFRVRATLPLDPPS"
     gene            2220..2876
                     /locus_tag="PLX85_03560"
     CDS             2220..2876
                     /locus_tag="PLX85_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007462555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC62283.1"
                     /translation="MTTVLIVDDQDLIREGFRGLIDVEPDLAVIGAAADGDEGVRLAR
                     ELRPDVVVMDIRMPIMDGLEATRRIRGDASLQDTHVLILTTFHLDEYVFAALRAGASG
                     FLLKDAPIAELRNAIRVVASGEALLAPSVTRTLIEEFIRRPDPPAHLPAAAFSELTAR
                     EVEIAGLVAEGLSNAEIAERLVLSHATVKTHISRILAKLDLRDRVQLVIAAFEAGLTA
                     "
     gene            2952..3530
                     /locus_tag="PLX85_03565"
     CDS             2952..3530
                     /locus_tag="PLX85_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004928482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRC62284.1"
                     /translation="MSATYTFDVFATLDGYGSYGPEGDWGGYWGKQGPELLQHRLAQY
                     EQAQRMVFGANTFRDFARMLGSSADLSDVDAWGLRMRDLPATVLSTTLAGPFDWPNAT
                     VVSGDPVEVVGRLKQESDLPLRSHGSLTLNWALMAAGLVDVVQVTVFPVISGRSGVDR
                     IFEGAADFDLELIESRTLDGRTQELLYRPSLH"
BASE COUNT          538 a         1531 c         1238 g          644 t
ORIGIN      
        1 accgctcgaa gtcgagcagt gtcatgccga cgtaccagcc gcggttctcc tcgcccacca
       61 gctgtcccgc gggcacgcgc acgttctcgt agaacgtctc gttggtcgcg tgctcgtacg
      121 ccattgagat gatcgggcgt acgctgacgc cctcggtgcg cgcgtccagc agcaggaacg
      181 agatgccgcg gtgcttcggc gcctccgggt ccgtgcggaa caacccgaag atccagttcg
      241 ccttgtgccc cgccgaggtc cacagcttct ggccgttgat cacgtattcg tcgccgtcgc
      301 gcaccgctcg acactgcagc gatccgaggt ccgagcccgc tcccggctcc gaaaacccct
      361 gcgcccacag gatctcgccc cgcagcgtcg gtcccatgaa ctgcttccgt tgttcgtccg
      421 tcccgtggac caggagcgtc gggccgatgt gcgtcacgcc cgctccgccg atcaccggcg
      481 cgttctgctg cgccatctcc tccgccagga tgtactgctc gagggctgac aggccgccac
      541 cgccgtactc ctttggccac tgcggcgcgc cccagccgcg ttccgccagc gcctctgccc
      601 agcgcttggc cgccgccttc gcctcatccg tcccgttcgc gaagtcctgc tgccagtcgt
      661 cctcgccgcc atgcactcgg ctgcggcccc ggtagtagtc tggcagccgt tcgctgatga
      721 acgagcgcgc cgaggcgcgg aattccgcct gctggggcga gtcgctccag tccatgatct
      781 cctccagccg tttccacctg cgcgattacg tgtggcgtga tgcccgcgcc gattctagct
      841 ccggcaggca agcacgcaca cgtacatctt cagatgtatg cccggacaca ccttgcgagg
      901 ggcgcacacc ggccccgcct gggccatgat gacgccatgc tcgaaagccg acgacgtcga
      961 tcgaccaagg cggcccccgt cgcacccgcc gaggacgtgc gttcgcgggg cttgatggcc
     1021 cgcctggaac gcacgctcgc tgagcgcgtg ggcgtgccac gccgcatcct ccggcaggcg
     1081 ggtgtcgtcg cggacgtgac catcgccctc gccctcttcg cggacgccat ggtcgcgctc
     1141 gccacgtcct cgcccggcgt cgagttcggc acgcgcgagc cggacgcctt cgcctacctg
     1201 ctcggcgccg tgatgaccct gccgttggcc gtccgtcggc gctggcccgt cgaggtcctc
     1261 ggcgtcgcgc tcgcggggca catcgccttc aacctccgcg actacgccgg cgtgaacgtg
     1321 gacttcttcg gtccaatcgt cgcgatgtac acggtcgctg ctcaccgccc ccggcgcgtc
     1381 tccgtcccgg cggccgccgt cgtgctgctc ggcgtgttca tcgccgatcc gccgttcgac
     1441 gaaccgctgg gtaacttcgt cgcaacggtg ctgatcatcg cgggcatctg gctcttcggc
     1501 gacgcggctc gcgtccgccg catctacggc gagcgcctcg ctgcccaggc cgaggcgctc
     1561 cgcgaggcgc gcatggagct cgcggaacag gccgtgaccc aggagcgcct acgcatcgcc
     1621 cgtgagctgc acgacgtcgt cgcccatcac atgagcgcca tcgtcgtcca gtccgccctc
     1681 gcgcaggacc gcctcgaggt cgatccgccc gccgctcgtc gcgccatcgg acaggtggag
     1741 tcgatgggcc gcggcgccct tcgcgagatg cgccaaatcc tcggcgtgct gcgtcaggct
     1801 ggcgaagatc gcggcgcgct cgacccctcg ccctccctcg gcgacctcga acggctctac
     1861 gcccgcgccc gcgacggcgg cctcaacgtc tcggctgcca tcgagggcga cctcagcggc
     1921 ctgccgccgg cactcgagct cgctgcctac cgcatcgtgc aggagtcgct cacaaacgcg
     1981 ctcaagtacg cccccggcag ccgcgtcgac gtccatctcg cccgcgatgc ccaccaggtc
     2041 acggtcgagg tcgtcgatga cggtccggcc ccgtcctcgc cgccgaccac ggctggcggc
     2101 gggcagggcc tcatcggcat gcgcgagcgc gtcgccctgc tggccggcaa cttcgaggcc
     2161 ggccggactc cctccggcgg cttccgcgtg cgggccacgc tgcccctgga tccgccctcg
     2221 tgaccaccgt cctcatcgtc gatgaccagg acctcatccg ggagggtttc cgtggtctca
     2281 tcgatgtcga gcccgatctc gccgtcatcg gcgctgccgc tgatggcgac gaaggcgtcc
     2341 gccttgcgcg cgagctgcgc ccggacgtcg tggtcatgga catccgcatg cccatcatgg
     2401 acggtctcga ggcgacacgc cgcatccgtg gcgacgcctc gctgcaggac acgcacgtcc
     2461 tcatcctgac cacgttccac ctcgacgagt acgtcttcgc cgccctccgc gccggcgcca
     2521 gcggcttcct gctcaaagac gcccctatcg ccgagctgcg caacgccatt cgcgtggtcg
     2581 cctccggcga ggcgctgctc gccccctccg tcacccgcac cctgatcgag gagttcatcc
     2641 gccgcccgga tccacccgct cacctccccg ccgccgcctt cagcgaactg accgctcgcg
     2701 aggtcgagat cgcggggctc gtcgcggaag gcctctcgaa cgccgaaatc gccgagcggc
     2761 tcgtcctcag tcatgccacc gtgaagaccc acatcagccg catcctcgcc aagctcgatc
     2821 tacgcgaccg cgtgcagctc gtcatcgccg ccttcgaggc aggcctcacc gcctagccgc
     2881 ccgaactgcc gcgggccttg gcaaatggtg tgaaaatggg gccgaggtcc ggacgacacg
     2941 ccggagacga catgagcgct acctacacgt tcgatgtctt cgccaccctc gacggctatg
     3001 ggtcgtacgg ccccgagggc gactggggcg gctactgggg taagcagggc cccgagctac
     3061 tgcagcaccg cctcgcgcag tacgagcagg cgcagcgaat ggtcttcggc gcgaacacct
     3121 tccgcgactt cgcgcgcatg ctcggctcga gcgccgatct ctcggacgtg gacgcgtggg
     3181 gcctgcgaat gcgggatctg cccgcgaccg tcctctcgac gacactcgcc ggccccttcg
     3241 attggccgaa cgccacggtc gtcagcggcg accccgtcga ggtcgtcggt cggctcaagc
     3301 aggaatcaga tctcccgctg cgctctcacg gcagcctcac gctgaactgg gcgctgatgg
     3361 ccgccgggct cgtcgacgtg gtgcaggtga ccgtctttcc cgtgatcagt ggccgctccg
     3421 gcgtcgaccg catcttcgag ggggcagccg acttcgacct cgagctcatc gagagccgga
     3481 ccctggacgg tcgcacacag gagctgctgt accgcccctc tctccactga cgcccgggcg
     3541 ggctgcgcgc cgcgcccatc cggcgtatct gccgacagcg gccccgcctg ccgtccccgc
     3601 gcgacataac tagatcgccg ccgctcctcg tacctcgttc ggatgacccg ctactccaac
     3661 cgccggccga cgccccggcg cttccgccta cgacctccgc tggcgcgcga agtgaccccc
     3721 tccgagggac gcgggcaccc acggtcgcgc gggatgctcc tcccgagacc gcagcgatag
     3781 cgggacggtc tcgcgcagag cccggcatca agaagcgggc agaggaggaa agcgatgaga
     3841 cggttcctgt ctactcgggc ggtcgccggc ggagcggtcc gccatccctg gctcaccgtc
     3901 ctgctgtggg tcgtgctgct gggcgcggcg tttgtctccg ccggcagcct g
//
LOCUS       DAORPG010000112         3889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1890_length_3889_cov_3.351069, whole genome shotgun sequence.
ACCESSION   DAORPG010000112 DAORPG010000000
VERSION     DAORPG010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1890_length_3889_cov_3.351069"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..361)
                     /gene="secG"
                     /locus_tag="PLX85_03570"
     CDS             complement(137..361)
                     /gene="secG"
                     /locus_tag="PLX85_03570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRC62285.1"
                     /translation="MELKDFLSFAQILISSVLIVLVLLQVKGTGFGAALGGQDSTFRT
                     RRGMQRSLHRMTIVLVGIFLLFSAWSVAAS"
     gene            complement(401..1729)
                     /locus_tag="PLX85_03575"
     CDS             complement(401..1729)
                     /locus_tag="PLX85_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HRC62286.1"
                     /translation="MSRSNITKWLYVGLQIKRWLLLLLFGFVVMALGVAYLLREVYLN
                     YPFPAWAATVTLQFIPRWLRGLLFISTASGITMYAAWRLNRSIVSAVLPVERQAQIFD
                     QIYAQRMQGRGPKIVAIGGGTGLSTMLRGLKQYTSNLTAVVTVADDGGSSGLLRRDMR
                     VIPPGDIRNCIAALADAEPLVTKLFQYRFDKDAGEGIAGHSFGNLFIVAMSEVTGNME
                     VAIRETSRVLAVRGQILPSTLADVTLSAVLEDGTTVRGESNIPHAGSPVASVFIEPAS
                     APVNPEVVRALLDADLIVIGPGSLYTSVLPNLLVRGLGEALLASRAHKVYIPNVATQH
                     GETDGYTAADHYAAIRRHLRSDRVVDVVLANSNLPAEPLPDSWQSRPVPAPGDADYGG
                     ARLVLADLVAPEHRYRHHPEQLAAAVMRQYYERDLRVQAASAVEPASIRS"
     gene            complement(1819..3309)
                     /gene="gatB"
                     /locus_tag="PLX85_03580"
     CDS             complement(1819..3309)
                     /gene="gatB"
                     /locus_tag="PLX85_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRC62287.1"
                     /translation="MTTAVATEYEVVVGLEVHVQLKTQSKMFCGCAADYFDVEPNTRV
                     CPVCLGMPGMLPVINRAAVEYTVLTGLALGCAIPQHSKFDRKNYPYPDLMKGYQISQY
                     DQPLCVGGGLEIEVDGVPHYVRLERIHLEEDTARLLHREDAHGEGYSLLDVNRSGVPL
                     MEIVSRPDIRSAAQAGAFLRKLRQTLRYLEVSDADMEKGSFRCDANVSIRPRGSETLG
                     AKVEVKNMNSFRAVMRAIESEVERQTEVLTSGGVVVQETRGYIDATGTTVSQRSKEEA
                     HDYRYFPEPDLPPLDITPAEVEELQRRLPELPDARKQRFEAEFGLTPSEAALLTETRA
                     EADSYEEAVALACPHGVDKGRARAVAHWFIGDVARLRHQEGGEVELSATPLTSAHIAE
                     LVTLVEGGTITLTTAKEVLEIAFESGSRPSAIVEERGLAQVRDEGEIDRIAREVIEAN
                     PKPVADYAAGKAAALQFLVGQVMRQTRGRADANSVADVLRRHLDAG"
     gene            complement(3447..3617)
                     /locus_tag="PLX85_03585"
     CDS             complement(3447..3617)
                     /locus_tag="PLX85_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62288.1"
                     /translation="MTMRTCFVTGQEFDDEKTGFKWMHDGKWYYFFDIGARSRFVGNP
                     QKYLDEAAAKAS"
BASE COUNT          633 a         1336 c         1262 g          658 t
ORIGIN      
        1 cggcgaccac ctcgatgtcc gcggaggcca gaagcaggcg cagggtcggc aatgcgtagt
       61 cgggcgaccc gaaaaagacg acacgcaatc ggcggccgcc gatgcgagga ccagaggtca
      121 cgtcaggcct cgtgcgctag gaggccgcga cgctccatgc cgaaaagagg aggaatatcc
      181 ccaccagcac gatcgtcatg cggtgcagcg agcgctgcat gccgcggcgc gtgcggaagg
      241 tcgagtcctg gccgccgagc gcggccccga agccggtgcc tttgacctgc agcagcacga
      301 gcacgatgag gacgctcgag atgaggattt gcgcgaagct gagaaagtcc ttgagttcca
      361 tagaccacca aggctatgcg ccaccgtcgc cgcgggcaag ttacgaccgg atcgaggccg
      421 gttccaccgc cgaggcggcc tgcacgcgaa ggtcccgttc gtaatactgg cgcatcacgg
      481 ccgctgccag ctgctccggg tggtgccggt atcggtgctc gggcgccacg aggtcggcga
      541 gcacgagccg ggcgccgccg tagtcggcgt caccgggtgc gggcaccggc ctcgactgcc
      601 acgagtcggg cagcggctcc gccggcaggt tgctgttcgc cagcaccacg tcgacgacgc
      661 ggtccgaccg caggtggcgg cggatcgccg cgtagtgatc ggccgccgtg tagccgtccg
      721 tctcgccgtg ctgcgtggcg acgttcggga tgtacacctt gtgcgcccgc gaggcgagca
      781 gcgcctcccc taggccacgc acgagcagat tcggcagtac cgaggtgtag agggatcccg
      841 gtccgatcac gatcagatcg gcgtcgagca gcgcgcgcac gacttcgggg ttcaccggcg
      901 cggaggccgg ttcgatgaat acgctcgcaa ctggcgagcc ggcatgcggg atgttcgact
      961 cgccgcgcac cgttgttccg tcctcgagga cggccgacag cgtgacgtcg gcgagtgtgg
     1021 aaggcaggat ctggccgcgc accgcgagca cccgcgaggt ctcccggatg gccacttcca
     1081 tgttcccggt cacctcggac atcgcgacga tgaacaggtt gccgaacgag tggcccgcga
     1141 tgccctcgcc ggcgtccttg tcgaagcggt actggaatag tttcgtcacg aggggttcgg
     1201 cgtcggccag cgctgcgatg cagttacgga tgtcccccgg cggaatcacg cgcatgtcgc
     1261 gacgcagcag gccgctcgat ccgccgtcgt cggctaccgt gacgaccgca gtcaggttgc
     1321 tcgtgtactg cttgaggccg cgcagcatcg tcgaaaggcc agtcccgcct ccgatcgcga
     1381 cgatcttcgg gccacgcccc tgcatccgct gcgcgtagat ctggtcgaaa atctgcgcct
     1441 ggcgttcaac cggcagcacc gccgacacga tcgatcgatt cagccgccag gccgcataca
     1501 tcgtgatccc cgaggcggtc gaaatgaaca gcaggccgcg caaccagcgg gggatgaact
     1561 gcagcgtgac cgtcgcggcc cacgccggga acggatagtt caggtacacc tcgcgcagca
     1621 ggtatgccac gccgagggcc atgacgacga agccgaatag cagcagcagc agccagcgct
     1681 tgatctgcag gccgacgtac agccacttgg tgatattgct gcgactcatg gacagcctcg
     1741 accgctgccc cgccgctgac cggtatacgt gccgcgctct gcgctcgtca acctgctaca
     1801 cccggacgta atgtccactc agccggcgtc gaggtggcga cgcagcacgt cggcgacgct
     1861 gtttgcgtcg gctcgccctc gcgtctgccg catgacctgg ccaacgagga actgcagcgc
     1921 cgcagccttt ccggccgcgt agtccgccac cggcttcggg tttgcctcga tgacttcgcg
     1981 cgcaatccgg tcgatctcgc cttcgtcacg tacctgcgcc agcccgcgct cctcgacgat
     2041 cgccgagggc cgggagccgc tctcgaacgc gatctcgagc acttccttcg cggtcgtcag
     2101 cgtaatcgtg ccaccctcta caagtgttac gagctcagcg atatgcgcgg atgtgagcgg
     2161 ggttgccgac aattcgacct ctccaccctc ctggtgccgc aaccgcgcca cgtctccgat
     2221 gaaccagtgc gcgacggccc gcgctcggcc cttgtccacg ccgtggggac acgccagggc
     2281 taccgcctct tcgtacgaat ccgcctcggc acgcgtctcc gtcagcagcg cggcctccga
     2341 gggcgtgagc ccgaactccg cctcgaaccg ctgcttgcgc gcgtctggca gctccggcaa
     2401 ccgccgctgt agctcctcga cctccgccgg cgtgatgtcg agcggaggca gatccggttc
     2461 cgggaagtag cggtagtcgt gcgcctcttc cttcgagcgc tgcgacaccg tcgtgccggt
     2521 cgcgtcgatg tacccacgcg tctcctgcac aacgacgccg cccgaggtga gcacctcggt
     2581 ctggcgctcc acttccgact cgatcgcgcg catcaccgcg cggaacgagt tcatgttctt
     2641 gacctcgacc ttcgcgccga gcgtctccga gccacgcgga cgaatcgaca cgttggcgtc
     2701 gcagcggaag gagcccttct ccatgtccgc gtccgacacc tcgaggtagc gcagcgtctg
     2761 ccgcagcttg cgcagaaatg ccccggcctg tgccgccgag cggatgtcag gcctcgacac
     2821 gatctccatc agcggcacgc cggagcggtt cacgtcgagc agggaatacc cctcgccgtg
     2881 cgcgtcttcg cggtggagga ggcgggccgt gtcctcctcg aggtggatgc gctccaaccg
     2941 gacataatgt gggacgccgt cgacctcaat ctcgagaccg ccacccacgc agagcggctg
     3001 gtcgtactgc gagatctggt agcccttcat gaggtcgggg taggggtagt tcttgcggtc
     3061 gaacttcgag tgctggggga tcgcgcagcc gagggcgagg cccgtgagga ccgtgtactc
     3121 caccgccgca cggttgatga cggggagcat ccccggcatt ccgaggcata cggggcacac
     3181 ccgcgtgttc ggctcgacat cgaagtaatc cgcggcgcag ccgcagaaca tcttcgactg
     3241 ggtcttgagc tgcacgtgga cctcgaggcc gacgacgacc tcgtattcgg tggcgactgc
     3301 ggtggtcata gcatccctgt ccgttgctgc accgattgta gcagcggact cgggtggccc
     3361 tcggagaccc tcggggggcc ggtaggggaa cctgcggcga ggagacagcc ctggccatct
     3421 cctcgccgcg tctggtgcgg gccggactag gacgctttcg ccgcggcctc gtcgaggtac
     3481 ttctgcgggt taccgacgaa gcggctccgc gccccgatgt cgaagaagta gtaccacttg
     3541 ccgtcgtgca tccacttgaa gccggtcttc tcgtcgtcga actcttgccc ggtcacgaag
     3601 cacgtgcgca tcgtcatgct ggttgcctcc ctgcgtctcc gatcgcgccc tgttcccggc
     3661 ctctcggccg cgagtgctcc gtattatatt cgtggaacgc aacgtttgtt atcgaaccag
     3721 tcccccgccc cgccgtcggc tgcgcctgct cacaaccggt cgaggcgcta gagtggtgcg
     3781 actatgctcg tctcgctcaa gtggcttcgc gactacgtcg acctgcctgc tgacctcgac
     3841 atcgatgacc tcgcgacccg cctcaccatg gcctcctccg aggtcgaag
//
LOCUS       DAORPG010000113         3859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1939_length_3859_cov_3.851472, whole genome shotgun sequence.
ACCESSION   DAORPG010000113 DAORPG010000000
VERSION     DAORPG010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1939_length_3859_cov_3.851472"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..511
                     /locus_tag="PLX85_03590"
     CDS             50..511
                     /locus_tag="PLX85_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019940806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRC62289.1"
                     /translation="MLPGVIQVPADLVIAASRKSSLWPMTFGLACCAIEMIATYMSHY
                     DFDRFGIVPWPSPRQSDVMIVAGTVTKKMAPAVKLLYEQMPNPKWVISMGACATNGGP
                     YTRYDRVVQGVDKLIPVDVYVPGCPPRPEALIDAFLALQEKIMEERAATGR"
     gene            508..1083
                     /locus_tag="PLX85_03595"
     CDS             508..1083
                     /locus_tag="PLX85_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRC62290.1"
                     /translation="MTTDDGAQQGSESGAAASAALDGTIWPEVDRALAGVTATAEPSV
                     LDVIVWIPRDEIVRGLRALKHDANLKFDYLRSLTCVDNEEEGMDVVYHLYSIEHGYNL
                     TVKAHLPVDDLNLDSATPVWRAANWYEREAAEMFGVRFRGHPDPRTLLMPEDMTDTFP
                     LRKDHPLAEIEVLQGEALAFPSGAGGGGEEE"
     gene            1083..2213
                     /locus_tag="PLX85_03600"
     CDS             1083..2213
                     /locus_tag="PLX85_03600"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRC62291.1"
                     /translation="MATQVHPDHEFDVQDLMINMGPQHPSTHGVFRMVLVVDGEIVRD
                     VVPYIGYMHRGGEKLAETMDYRQGIGWMDRTEYLAQFNAELSYVMAVEALAELQVPDR
                     AQWIRMILCELNRILSHFMFTGAFGTDVGVFGTAFTYSFREREVIQDLFEEITGDRMM
                     YAYFRPGGLAWEVPDNFVPRVREVLKSVRQGIKDMNGLLTDNEVFRARTVGVGAISAA
                     DATDWGCTGPVLRSTGVPLDLRRTEPYLKYDEIDFDVITGTTGDVFERFTCRIGEMWE
                     SIRIVEQCLERLEPGPIMPDRMPRMLRTPPGEIYMRTEAPRGEYGIYLVSKGGNRPHR
                     LKIRSSCFANLQALRDMTIGSYVADAVVILGSLDIVLGEVDR"
     gene            2210..3343
                     /gene="nuoH"
                     /locus_tag="PLX85_03605"
     CDS             2210..3343
                     /gene="nuoH"
                     /locus_tag="PLX85_03605"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRC62292.1"
                     /translation="MNNELFGKDINVWIDAFIRVNIITIMMTVVVMGLIYLERKAIAR
                     FQMRLGPTRTGPMGLLQSVADALKLVAKEDVRPRNADPWIFELAPYFVFVPVFLMFVA
                     IPFAVSWEVRVLDLGLFYVLAVSSVNIVGWVMAGWGSDNRYAMLGALRAAAQSISYEL
                     PLVLSMIAIAMVANSLNLSEIVAGQGRIPNIILQPLPFVIFYIAMLAELNRTPFDIPV
                     GESEMVGGPYVEYSGIRWSFFFLAEYASLFILALLASAVFLGGWAWPLGEDWGRWWQF
                     ILTGVKAGAMIFSVFWVRSTLPRLRIDQLMTFSWKVLLPLSFIQIMVNGAILVYEPEK
                     EVGQTLLGVTSVFGVFFLAWIINRSVTRPRPRPVVAARGEAVP"
     gene            3340..3810
                     /locus_tag="PLX85_03610"
     CDS             3340..3810
                     /locus_tag="PLX85_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRC62293.1"
                     /translation="MKGLLKSMGVTLSAFTRKPVTREYPETHKQLPERDRAFPILLWD
                     HGMNEPFCTGCHACERACPVECMTVLMKDNPNHKSQGGESSRRKIIDKFWIDYGRCMR
                     CNICVEVCNFDAIAMNNTWQGHEKSVYDRSDLVQELPQLLAVHRSGELKPWVVP"
BASE COUNT          636 a         1267 c         1237 g          719 t
ORIGIN      
        1 gcgcctgacg ccggtcgagt accgaccggg ccgcgcgctc tacaagacgg tgctccccgg
       61 cgtcatccag gtgcccgccg acctcgtcat cgcggcctcg aggaaatcct cgctgtggcc
      121 gatgaccttc ggcctcgcct gctgcgcgat cgagatgatc gcgacctaca tgtcgcatta
      181 cgacttcgac cgcttcggca tcgtgccgtg gccgtcgcct cggcagtcgg acgtcatgat
      241 tgtcgccggt acggtgacga agaagatggc acccgcggtg aagctgctct acgagcagat
      301 gccgaacccc aagtgggtca tctcgatggg cgcgtgcgcc acgaacggtg ggccctacac
      361 ccgttacgac cgcgtcgtac agggcgtcga caagctcatc ccggtggacg tctacgttcc
      421 gggctgcccc ccgcgaccgg aggcgctcat cgacgccttc ctcgcactcc aggagaagat
      481 catggaggaa cgggcggcga ccggccgatg accaccgacg acggcgcgca gcagggttcc
      541 gagtccggcg ccgcggccag cgcggccctc gacggcacga tctggccgga ggttgaccgc
      601 gccctagcgg gcgttacggc cacggcggag ccgagcgtcc tcgacgtgat cgtctggatc
      661 ccgcgtgacg aaatcgtccg cggactgcgc gcgctcaagc acgacgcgaa cctgaagttc
      721 gactacctgc gctccctgac gtgcgtcgat aacgaggagg aggggatgga cgtcgtctat
      781 cacctctact ccatcgagca cggctacaac ctcaccgtca aagcgcacct gccggtggac
      841 gacctcaacc tcgacagcgc gactcccgtg tggcgcgccg cgaactggta tgagcgcgag
      901 gccgccgaga tgttcggcgt ccgcttccgc ggccatccgg acccgcgcac gctcctgatg
      961 cccgaggaca tgaccgacac gttccccctg cgcaaggatc atccgctcgc ggagatcgag
     1021 gtgctgcagg gcgaggcgct cgcgttcccg tccggcgccg gtggtggcgg ggaggaagag
     1081 tagtggcgac gcaggtccat cccgaccacg agttcgacgt ccaggacctc atgatcaaca
     1141 tggggccgca acacccctcg acgcacgggg tgttccgcat ggtgctcgtc gtggacggcg
     1201 agatcgtgcg cgacgtcgtg ccgtacatcg gctatatgca ccgaggcggc gagaagctcg
     1261 ccgagaccat ggactaccgc cagggcatcg gctggatgga ccgcaccgag tatctggcgc
     1321 agttcaatgc cgagctctcc tacgtgatgg ctgtcgaggc gcttgcggag ctgcaggtgc
     1381 ccgaccgggc gcagtggatc cggatgatcc tctgcgagct gaaccgcatc ctgagccact
     1441 tcatgttcac cggtgcgttc ggcacggacg tcggtgtctt cggcacggcc ttcacgtatt
     1501 cgttccggga acgcgaagtg atccaggacc tcttcgagga gatcaccggc gaccggatga
     1561 tgtacgccta cttccggcca ggcggtcttg cctgggaggt tcccgacaac ttcgtgccgc
     1621 gcgtacgcga ggtgctgaag tcggtccggc aaggcatcaa ggacatgaat ggcctgctga
     1681 ccgacaacga ggtgttccgc gcgcgcaccg tcggtgtggg tgcgatctcg gcggcggacg
     1741 cgaccgactg gggctgcacc ggtccggtgc tgcggtcgac gggcgtgccg ctcgacctgc
     1801 gccgcaccga gccgtacctc aagtacgacg agatcgactt cgatgtcatt accggcacga
     1861 ccggcgacgt cttcgagcgc ttcacgtgcc gcattggcga gatgtgggag tcaatccgca
     1921 tcgtcgagca gtgcctcgag cggctcgaac ccggcccgat catgccggat cgcatgccgc
     1981 gcatgctgcg cacgccgccg ggtgagatct acatgcgcac cgaggcccct cgtggcgagt
     2041 acggcattta cctcgtgagc aagggcggca accgccccca ccgcctcaag atccgctcgt
     2101 cctgcttcgc caacctgcag gcgctgaggg atatgaccat cggttcttat gtcgcagacg
     2161 ccgtggtgat cctcggctcg ctcgacatcg tactgggcga ggtcgaccgg tgaataacga
     2221 gctcttcggc aaggacatca acgtctggat cgatgcgttc atccgtgtga acatcatcac
     2281 aatcatgatg accgtggtcg tcatggggct gatttacctc gaacgcaagg cgatcgcgcg
     2341 cttccagatg cgactcgggc cgacgcgcac gggcccgatg ggtctgctcc agtcggtggc
     2401 tgatgcgctc aaactggtgg cgaaggaaga cgtacgtcct cgtaacgctg acccctggat
     2461 cttcgagctc gccccctact tcgtcttcgt ccccgtcttc ctcatgttcg tcgccattcc
     2521 cttcgcggtg agctgggaag tgcgtgtcct tgacctcggc ctcttctacg tgctcgcggt
     2581 ttcatccgtg aacatcgtcg ggtgggtcat ggccggctgg ggctcggaca accgctacgc
     2641 catgctgggc gccctgcgcg ccgcggccca gtcgatttcg tacgagctgc ccctggtgct
     2701 ctcgatgatt gccatcgcga tggtggcgaa ctcgctgaac ctgagcgaga ttgtcgccgg
     2761 ccagggccgc atcccgaaca tcatcctgca gccgctgccc ttcgtgatct tctacatcgc
     2821 gatgctggct gagctgaacc gcaccccctt cgacatcccg gttggtgagt cggagatggt
     2881 gggtggtccc tacgtcgagt acagcggcat ccgctggtcg ttcttcttcc ttgcggaata
     2941 cgcctcgctg ttcatcctgg cgctgctcgc ctctgcggtg ttcctcggcg gttgggcgtg
     3001 gccactgggc gaggactggg gccgctggtg gcagttcatc ctcacggggg tgaaggccgg
     3061 ggccatgatc ttctccgtgt tctgggtgcg ctcgacgttg cctcgtcttc gtatcgacca
     3121 gctgatgacc ttctcctgga aggtgctgct cccgctgtcg ttcatccaga tcatggtgaa
     3181 cggcgccatc ctcgtgtacg agcccgagaa ggaagtcggc cagaccctgc tcggcgtgac
     3241 gagcgtgttc ggcgtcttct tcctggcctg gatcatcaac cgcagcgtca cccgaccgcg
     3301 cccgcggccg gtggtcgccg ctcgaggtga ggcggtccca tgaaaggcct gctgaagagc
     3361 atgggtgtga cgctctccgc gttcacccgc aagcccgtca cccgcgagta cccggagact
     3421 cacaagcagt tgccggagcg cgaccgcgcg ttccccatcc tgctctggga tcacggcatg
     3481 aacgagccgt tctgcacggg ctgccacgcc tgtgagcgag cgtgtcccgt tgagtgcatg
     3541 accgtcctga tgaaggacaa cccgaaccac aagtcgcagg gcggcgagtc ctcgcggcgc
     3601 aagatcatcg acaagttctg gatcgactat ggtcgctgca tgcgctgcaa catctgcgtc
     3661 gaggtctgca acttcgacgc gatcgcgatg aacaacacgt ggcagggtca cgagaagtcg
     3721 gtctatgacc gcagcgacct ggtccaggaa ttgccgcagc tgctcgcggt gcaccgcagc
     3781 ggcgagttga agccgtgggt ggtcccctag tgatgcagcg aattgccctt ccctcgcaga
     3841 tcgaccgcgt cattgtcgg
//
LOCUS       DAORPG010000114         3849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1951_length_3849_cov_3.290722, whole genome shotgun sequence.
ACCESSION   DAORPG010000114 DAORPG010000000
VERSION     DAORPG010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1951_length_3849_cov_3.290722"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="PLX85_03615"
     CDS             complement(<1..1308)
                     /locus_tag="PLX85_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC62294.1"
                     /translation="MLDSDREHPLRLTDRFGRRDLVRGGALAGAGLAAAVLIGCGGDD
                     DNEPSGASTSPGATGTSAPTAFSGITTAKGRQIPYNFAEPAKAPKSGGTMRQAYTFDP
                     GQLDPTASQAGGTLTAVNAAYDRLINVNGRADADPTKINQLEPGLASKWETSPDGLTY
                     TFSLQQNVKFQNLAPLNGRLFVAEDARFALNRYKTEGVNQQFFTQVDTITAPDTKTLV
                     IKMKRPQPDFIFPLSTAYTTIHSHELVDDGSIKSKAIGTGPMIVDKWQSGTGGAFVRN
                     PDYWAGPTYLDRFELPYILDPAANTAAFRASQVDWGITIGTEDDFENVLGTNPNIQYI
                     TAPVISATFAIAFNNELPMWKDERIRRAVSLTVDRPTLSQLVYKALGKSVPVPDWRFF
                     WDQEPTFESGHLGKWWKTDIAQAKQLLQAAGQENFSFEMVYYNY"
     gene            complement(1446..1817)
                     /locus_tag="PLX85_03620"
     CDS             complement(1446..1817)
                     /locus_tag="PLX85_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62295.1"
                     /translation="MVKQAVARPFVMLVAAGVIVLAGVAVAGAASVWPLKSHQSAGPV
                     ASNLNTASQKSVGTTGTFVDVTDNPVSLPSEKTIYPPRTGNAGLATPHQVPNTAVPPL
                     LAATAILLLAARFATRHHLSR"
     gene            complement(1944..2876)
                     /locus_tag="PLX85_03625"
     CDS             complement(1944..2876)
                     /locus_tag="PLX85_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRC62296.1"
                     /translation="MPDCTVMTWNVENLFPVGHLSGAASVDVYQRKLRYLAKTIRDVG
                     PDVIGLQEVGDPTCLADLQAALGGRYPHAAISSRPDIRGIRVAVLSASPLTDVRQLFE
                     FTPEALPRVPTADGKTVATMGRGALQVVVEVSGRRLRVVTAHLKSKLLSYPEGRRFPK
                     DEHERARGAGFALLRRAAEAVALRVHLNTLMADEALPTVLVGDFNDGPSAITTDLLQG
                     PPDTSMRRPDKGDLVRLNNLAGWLPGKHAFSRMFREEPELIDHILVSQDILRGFRQVD
                     SLVDGVRSITEDVESRRNAVRPDHAPLFARFVLP"
     gene            complement(2950..3480)
                     /locus_tag="PLX85_03630"
     CDS             complement(2950..3480)
                     /locus_tag="PLX85_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62297.1"
                     /translation="MQRLLHLASARRAMLPLLLAVALLISLAAARVAEAQFLPAVLYG
                     GSLKAGQKVEALVDGAVCASTTASPKGEWVMQVSPDSPCKPKPGAAISFKVDGAAATS
                     NPAATWESGGIPKGSVQTGYTLTVGGGSSGASGGSSSESGDSGSNPTLFIVVGVVLLA
                     VVGGAGWFVYQRRSTN"
     gene            3706..>3849
                     /locus_tag="PLX85_03635"
     CDS             3706..>3849
                     /locus_tag="PLX85_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRC62298.1"
                     /translation="MARVEKNIEVAVPVRTAYNQWTQFEQFPRFMEGVKEVRQLDDRH
                     LHWR"
BASE COUNT          600 a         1169 c         1418 g          662 t
ORIGIN      
        1 gtagttgtaa tagaccatct cgaaggagaa gttttcctgc cctgcggcct gcaggagttg
       61 cttggcctgc gcgatgtccg tcttccacca cttgccgagg tgtcccgact cgaaggtggg
      121 ttcctggtcc cagaagaagc gccagtcggg aaccgggacg gacttcccca gcgccttgta
      181 gaccagctgg ctgagggtgg gccggtcgac cgtcagcgag accgcgcggc gaatccgctc
      241 gtccttccac atcggtagct cgttgttgaa tgcgatcgcg aacgtcgcgc tgatcacggg
      301 cgcggtgatg tactgaatgt tcggattcgt tccgagcacg ttctcgaaat cgtcctcggt
      361 accgatggtg atgccccagt cgacctggct cgcgcggaac gcggccgtgt tcgcggcggg
      421 gtcgaggatg tagggcagct cgaagcggtc gaggtaggtc gggcccgccc agtagtccgg
      481 gttgcggacg aatgcgccgc ccgtccccga ctgccacttg tcgacgatca tggggccggt
      541 gccgatggcc ttgctcttga tgctgccgtc gtcgaccagc tcgtgactgt ggatcgtcgt
      601 gtaggcggtg gacaacggga agatgaagtc cggctggggg cgtttcatct tgatgaccag
      661 cgtcttcgtg tcgggggccg tgatggtgtc gacctgggtg aagaactgct ggttcacgcc
      721 ctcggtcttg taacggttca gggcgaagcg ggcgtcctcc gcgacgaaga ggcggccgtt
      781 cagcggggcg aggttctgga acttcacatt ctgctggagg gagaacgtgt aggtgagccc
      841 gtccgggctg gtctcccact tcgacgcgag tccgggctcg agctggttga tctttgtcgg
      901 gtccgcgtct gcccggccgt tcacgttgat cagccggtca tatgcggcgt tgacggcggt
      961 gagggtgcct ccagcctgcg aggcggtcgg gtcaagctgg cccgggtcga aggtgtaagc
     1021 ctgccgcatc gtcccgcccg acttgggcgc tttcgccggc tcggcgaagt tgtaggggat
     1081 ctggcggccc ttcgcggtgg tgatgccgct gaacgcggtg ggcgcgctcg tgccggtagc
     1141 gccgggggac gtcgaggcgc cggagggctc gttgtcgtcg tcgccaccgc agccgatcag
     1201 gacggcggcg gccaggcccg cgccggcgag cgcgccacca cgcacgaggt cgcgacgccc
     1261 gaatcgatcg gtcagacgca gcgggtgttc tcgatcgctg tccagcaagg tatccctcct
     1321 cgtgaggcat gtgcctcgac tgcgggccgc cggcggcggt ccgggaggtc actataacgt
     1381 atgcatgcgc atcgtttgtg atatgtgcgg ccgtggagta cgcccaaggg tccgagggcg
     1441 gccggctacc tcgagaggtg gtgacgggtg gcgaaccgag ccgccagcag gaggatggcc
     1501 gtcgcggcga gcagcggcgg aactgccgtg ttggggacct ggtggggggt cgcgaggccg
     1561 gcgttaccgg tgcgtggcgg gtagatcgtc ttctcgctcg ggagcgagac cgggttgtcg
     1621 gtcacgtcca cgaacgtgcc ggtcgtaccg accgacttct gggaagccgt gttcaagttc
     1681 gaggcgacgg ggcccgcgga ctggtgcgat ttcagcggcc agacggaagc tgccccggcg
     1741 acggcgacgc ctgccagcac gatgacgccg gccgcgacca gcatgacgaa cggccgcgcc
     1801 actgcctgct tgaccaccgg cctgcctgct tcctcggagc gctgcccgca acccctctac
     1861 ccagtaatag cagaaaccag atgggatgtg ggctccagtt cctcgccctg acgcgccggc
     1921 gaagaggagg ggcggtgtgg cggtcagggc aacacgaagc gggcgaatag cggagcgtga
     1981 tcggggcgga cggcgttgcg ccggctctcg acgtcctcgg tgatcgaacg gacgccgtcg
     2041 acgaggctgt cgacctggcg aaagcctcgc aggatgtcct ggctgaccag gatgtggtcg
     2101 atcagctcgg gttcctcgcg gaacatgcgg ctgaacgcgt gcttcccggg gagccagccc
     2161 gcgaggttgt tcaaccgcac gaggtcgcct ttgtccggtc tccgcatcga ggtatcgggg
     2221 ggcccctgga gcaggtcagt ggtgatcgcg ctcgggccgt cgttgaagtc gcccaccagg
     2281 acggtcggca acgcttcgtc agccatgagt gtgttgaggt ggacgcggag ggcgacggcc
     2341 tcagcggctc gacgaaggag ggcgaagccc gcaccacggg cacgctcgtg ttcgtccttc
     2401 gggaaacggc ggccttccgg gtacgacagc agcttcgact tcaaatgggc cgtgacgaca
     2461 cggagacggc ggcccgacac ctcgacgacg acctggagcg cgccgcgccc catggtggcg
     2521 actgtcttcc cgtcggcggt ggggacgcga ggcagcgcct ccggagtgaa ctcaaacaac
     2581 tgtcggacgt cggtcagtgg actggcggac agcaccgcga cccgaatgcc tcggatgtcc
     2641 gggcggctgg agatggcggc gtggggataa cggcccccga gagcagcctg cagatcagcg
     2701 agacaggtgg ggtcgccgac ttcctgtagc ccgatgacat cggggccgac gtctcgaatc
     2761 gtcttcgcga gatacctcag cttgcgctgg tatacgtcca cagaggcggc gccggaaaga
     2821 tggcccacgg ggaagaggtt ttcaacattc caggtcatca cggtgcagtc gggcatggcg
     2881 gacctcccgt ccgagcagtt cgctcggcgg tagggtacgc ctcgaggcgg cggcgcgggc
     2941 ttgtttctgt tagttcgtcg agcggcgctg gtacacgaac cagccagccc cgccgacgac
     3001 ggcgaggagc accacaccta cgacgatgaa gagcgtggga ttgcttccgc tgtcgccgct
     3061 ctcggaggag gagccgccgc ttgccccgct cgagccgcca ccaacggtca gcgtgtaccc
     3121 cgtctgcacg ctgcccttgg ggatgccacc cgattcccag gtagcggcgg ggttggaagt
     3181 cgcggcggca ccgtcgacct tgaagctgat ggccgcgccg ggcttcggct tgcacggcga
     3241 gtcggggctg acctgcatca cccactcgcc cttcggcgag gcggtcgtcg aggcacatac
     3301 cgcgccgtcg accagggcct cgaccttctg accggctttg aggctgccgc cgtacaagac
     3361 cgcggggagg aactgggcct cggcgacgcg ggcggcggca agggagatca gcagcgcgac
     3421 ggcgagcagc aggggcagca tcgcgcggcg cgcagacgcc aggtgcagca agcgttgcat
     3481 cggttcctcc tcgtgccgtg tcgctcgggc gccggctggc gcgtcgaata ttgtgcacgt
     3541 cgcgcgtggt gtcgtcgcgg ggaatcacga tcgtgagggg gcatgggcgg ggcgggcgat
     3601 gcgggtagtc acgcctctga aagccggggc ggacgcccgc ggctacggct gcaagcgcgc
     3661 cttccctacg ctcgtacgtg gagtgggtag gggaaggttt gatccgtggc gagggtcgag
     3721 aagaacatcg aggtcgcggt gccggtccga actgcgtaca accagtggac gcagttcgag
     3781 cagtttccgc ggttcatgga gggtgtcaag gaagtccggc agcttgatga ccgccacctg
     3841 cactggcgg
//
LOCUS       DAORPG010000115         3827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1978_length_3827_cov_4.250530, whole genome shotgun sequence.
ACCESSION   DAORPG010000115 DAORPG010000000
VERSION     DAORPG010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1978_length_3827_cov_4.250530"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PLX85_03640"
     CDS             complement(<1..681)
                     /locus_tag="PLX85_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRC62299.1"
                     /translation="MAGARFTIAAPANGYNASLKVSTFSLPGLAASIPTDTVGAPVTP
                     PAVAVTVVPAAEQPPVVVSAEPVSTPESVRQVSQAVVEGTAVPAAAAPVRASRTQEPV
                     LKYYQYQVQPGDTVNAIASRSGIDPKYILWNNIDIISNEHVLTVGDILMIPAVEGILH
                     PVRIGDTLSGIATQYDAKVADIVAFAGNGLANPGDLKEGSIILVPGGRMVDRSTPGAP
                     TAKPTPPPQ"
     gene            complement(1059..2432)
                     /gene="rho"
                     /locus_tag="PLX85_03645"
     CDS             complement(1059..2432)
                     /gene="rho"
                     /locus_tag="PLX85_03645"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRC62300.1"
                     /translation="MARGPSRGRASSAPAPEFTGDQPPREERRNGQRGDAPTSLNIAE
                     LERRSREDLIDYAAALEIENAAALPKQELIFRILQDQAERHGNIFSGGVLAVVDDGFG
                     FLRGERLIPGPNDVYVSQSQIRRFGLRNGDYVTGQVRQPKDSEKYYGLLRVEAVNGLD
                     PEDAKRRPYFENLTPIFPNRQLTLESDSTELSQRIVDLFAPVGRGQRGLIVSPPKAGK
                     TMLLKSIAHGVVMNNPDVHLMVLLIGERPEEVTDMQRSVRGEVIASTFDEPVEDHTRV
                     AEAAIERARRLVEGGTDVVILLDSITRLARAYNLAIPTSGRTLSGGMDPIALYPPKRF
                     FGSARNTEEGGSLTIIATCLVETGSRMDDVIFEEFKGTGNMELRLDRRLAEKRVYPAI
                     DIFASSTRREDLLFDEETLRQVWLLRRMAAMVGDQGTDATERVLERLAKTKNNQEFLA
                     TLKSEVI"
     gene            complement(2564..3211)
                     /gene="fsa"
                     /locus_tag="PLX85_03650"
     CDS             complement(2564..3211)
                     /gene="fsa"
                     /locus_tag="PLX85_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HRC62301.1"
                     /translation="MRLFLDTANIDDIRRGAAMGLVDGVTTNPTLVAKEGVEYRDRVL
                     EICEAVNGPISAECISTTADALVEEARRIASWHPNVIVKVALTEQGLQAIRRLSAEGI
                     TINTTLVFSANQALLAAKAGAAYVSPFVGRLDDAGEDGMQVVRESVQIFETYHLPTQV
                     LAASIRSARSVTQAALAGSHVATVPPNIFFQMIQHPLTDVGIERFLADAAKYTPV"
     gene            complement(3257..3727)
                     /locus_tag="PLX85_03655"
     CDS             complement(3257..3727)
                     /locus_tag="PLX85_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62302.1"
                     /translation="MTWHLLHTLALFWMMVGIGAVVVPVWKAWATKDLDARALLLQEA
                     QRSEVQWLIPGMLATLFTGVAWVSSDESTYNFVRTGWLAALEIVFAIDGFIFLPLMGV
                     GLRRVRLLALQAKHQGRMTEELDEALRDNVPVVFGTLLTLSIPVMVWLAIYQPI"
BASE COUNT          619 a         1296 c         1280 g          632 t
ORIGIN      
        1 ctgaggcggc ggcgtcggct tcgccgtagg cgcgccgggc gtgctgcggt ctaccatgcg
       61 gccgcccggc acgaggatga tcgagccttc cttgaggtca ccggggttgg ccagcccgtt
      121 gccggcgaac gccacgatgt ccgccacctt cgcgtcgtac tgcgtggcga tgcccgacag
      181 cgtgtcgccg atgcgcaccg ggtgcaggat gccttcgacg gccgggatca tgaggatgtc
      241 ccccaccgtc agcacatgct cgttcgagat gatgtcgatg ttgttccaga ggatgtactt
      301 cggatcaatg ccggagcgcg acgcgatcgc gttcaccgtg tcgcctggct gaacctggta
      361 ctggtagtac ttgagcaccg gctcctgcgt gcgcgaggcc ctcacgggcg ccgcggcggc
      421 cggcaccgcc gtgccttcga cgaccgcctg ggatacctgg cggacggact caggcgtcga
      481 cacgggctca gccgagacca ccaccggggg ctgctcggct gccggcacca cggtgaccgc
      541 aaccgctggc ggcgtcaccg gagcacctac ggtgtcggtc ggaatcgagg cggcgaggcc
      601 cggcagcgag aacgtggaga ccttcagcga ggcgttgtag ccgttggcgg gcgccgcaat
      661 ggtgaagcgc gcaccagcca ccacaaccgc cagcagggcg atcgcgacgg ccgcgtgggt
      721 gatgaggcga agcgcgtagc gtctcccgtg gggacgcgtg tgcctcctgg ccctggccag
      781 cgttcgactg cgacgcgggc gctcggatcg cgcccgcacc gttccgtgat cgggaatacc
      841 gccgcccctt ttttatcgaa cctcgcgcca cccgcctcgg tgctttcgca ctcccgctcc
      901 cggcggcgca acggggggaa gtctaccacg cctcaacaag tcatcgcgac gtaatttctc
      961 cacaacgatt acgtcgggaa tgcgatggat ccgatagatc gagacataac aagatgcagg
     1021 ggcggcacgt cgaaacccga gccgcaggcc taccggagct agatgacctc ggacttcaga
     1081 gtggcgagga actcctggtt gttcttcgtc ttggccagcc gctcgagcac gcgttccgtg
     1141 gcgtccgtgc cctgatcgcc gaccatcgcc gccatgcggc gcagcagcca cacctggcgc
     1201 agtgtctcct catcgaagag caggtcctcg cggcgggtgg aggaggcgaa gatgtcgatc
     1261 gcggggtaga cgcgtttctc ggccaggcgc cggtcgagcc gaagctccat gttcccggtg
     1321 cccttgaact cctcgaagat gacgtcgtcc atgcgtgacc cggtctcgac gaggcaggtc
     1381 gcgatgatcg tcagcgatcc gccctcctcg gtgttccggg cggaaccgaa gaagcgcttc
     1441 ggcgggtaca acgcgatcgg gtccatacca ccggacagag tgcgcccgct ggtcgggatc
     1501 gcgaggttgt aggcccgcgc gaggcgcgtg atcgagtcga gcaggatcac gacatccgtg
     1561 cccccctcga cgaggcgtcg ggcacgctcg atggcggcct ccgccacgcg ggtgtggtcc
     1621 tcgaccggct cgtcgaacgt cgaggcgatc acctcgccgc gcaccgagcg ctgcatgtcc
     1681 gtgacctcct cgggccgctc accgatgagc agcaccatga ggtgcacgtc cgggttgttc
     1741 atcaccacgc cgtgcgcgat cgacttcagc agcatcgtct tgcccgcctt cggcggcgag
     1801 acgatgaggc cgcgctgccc acgcccgacg ggtgcgaaca ggtcgacaat gcgctggctg
     1861 agctcggtgc tgtcgctctc gagtgtcagc tgacggttgg ggaagatcgg ggtcaggttc
     1921 tcgaagtacg gtcgccgctt ggcgtcctcc gggtcgaggc cgttcaccgc ctcaacgcgc
     1981 agcagcccgt agtacttctc ggagtccttc ggctgccgca cctggccggt gacgtagtcg
     2041 ccattgcgca gcccgaagcg ccggatctgc gactgcgaga cgtagacgtc gttcggaccg
     2101 ggaatcagcc gctcgccgcg caggaacccg aagccgtcat cgacgaccgc gagcaccccg
     2161 cccgagaaga tgttcccgtg ccgctctgcc tggtcctgca ggatgcggaa gatcagctcc
     2221 tgcttcggca gcgcggccgc gttctcgatc tcgagcgcgg ccgcgtagtc gatcaggtct
     2281 tcgcggctgc ggcgctcgag ctccgcgatg ttgaggctgg tcggcgcgtc tccgcgctga
     2341 ccatttcgcc gctcctcgcg aggcggctga tcgcccgtga attctggtgc aggcgcgctc
     2401 gacgcgcgtc cgcgggaagg acctcgtgcc atgtggttaa gagttgccgt ctgctacgtg
     2461 tcgccgccgt ggaacctgag cggtgacttc cagcgatgtg gacgtattca gtctacagac
     2521 cacctttccg ttcagcaagc gaaccgcacg ggggcgcgca cgcctagacc ggtgtgtact
     2581 tggccgcgtc cgcaaggaac cgctcaatgc ccacgtcggt cagcgggtgt tgaatcatct
     2641 gaaagaagat gttcggcggc accgtcgcga cgtgcgagcc cgcgagggcg gcctgcgtga
     2701 cggaacgcgc gctgcggata ctcgcggcga ggacctgcgt cggcaggtgg tatgtctcga
     2761 agatctgcac cgattcgcgc acgacctgca taccgtcctc gcccgcatcg tcgaggcgcc
     2821 cgacgaacgg agagacgtag gcggcgcccg ctttcgccgc cagcagcgcc tggttcgccg
     2881 agaacaccag cgtggtgtta atcgtgatcc cctccgccga cagccggcgg atcgcctgca
     2941 ggccctgctc cgtcaacgcc accttcacga tcacgttcgg gtgccacgag gcgatccggc
     3001 gcgcctcctc gaccagcgca tccgccgtcg tggagatgca ttccgccgag atcggcccgt
     3061 tgacggcctc gcagatctcg agcacgcggt cgcggtattc cacgccctcc ttcgcgacga
     3121 gcgtggggtt cgtcgtcacc ccgtccacga ggcccatggc ggcgccccgg cggatgtcgt
     3181 cgatgtttgc agtatcaaga aacagtcgca tgtgcgtcgc ctttcgaggg gcgccggctc
     3241 atgccgcgcc cgaacgtcag atcggctggt agatcgcgag ccagaccatc accggtatag
     3301 acagtgtaag caacgtgccg aagaccacgg ggacgttatc gcgcagcgcc tcgtcaagtt
     3361 cctcggtcat ccggccctgg tgcttcgcct gcagggcgag caggcgcacg cggcgcaggc
     3421 ccacccccat cagcggcagg aaaatgaacc cgtcgatggc gaagacaatc tcgagggccg
     3481 cgagccatcc cgtgcggacg aagttgtacg tcgactcatc cgaggagacc caggccacgc
     3541 cggtgaagag tgtcgccagc atgccgggga tcaaccactg cacctcgctg cgttgcgcct
     3601 cctgcagcag cagcgcccgc gcgtcgaggt ccttcgtggc ccaggccttc cagaccggca
     3661 cgacgacggc gccgatgccg accatcatcc agaacagcgc gagcgtatgc agaaggtgcc
     3721 aggtcacgcg ctggcaaccg ctcctgtagc cgcggccgac ccggcacgct cgagcgcggc
     3781 gccgatgaag tcgcggaaca gcgggttcgg ccggttgggc cgcgagg
//
LOCUS       DAORPG010000116         3821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_1987_length_3821_cov_3.124801, whole genome shotgun sequence.
ACCESSION   DAORPG010000116 DAORPG010000000
VERSION     DAORPG010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1987_length_3821_cov_3.124801"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..483)
                     /locus_tag="PLX85_03660"
     CDS             complement(40..483)
                     /locus_tag="PLX85_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62303.1"
                     /translation="MIWNRVWRSPVSGIAVGVLIASAVFGGFNLVRAQSTSTNTAQIE
                     ACANRYTGIIRVVGHIPCTAFEYPITLNQQGPAGPSGPKGDTGPTGPTGPTGPSGPLG
                     PIGMTGPTGASGPIGSTGASGPVGVTGPSGPVGESGPTGPTGIPG"
     gene            complement(565..798)
                     /locus_tag="PLX85_03665"
     CDS             complement(565..798)
                     /locus_tag="PLX85_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HRC62304.1"
                     /translation="MAGDEGRSYRIVELVGTSPDGIEAAIDNGIKAAGEAVDSLDWFQ
                     VREIRGRIENKGVAWYQVTLGVGFRVDDASQVI"
     gene            1002..2663
                     /gene="argS"
                     /locus_tag="PLX85_03670"
     CDS             1002..2663
                     /gene="argS"
                     /locus_tag="PLX85_03670"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRC62305.1"
                     /translation="MIRDDVQRLVVEALTAAQQAGEIPLFAPPEIQVEHPARAEHGDY
                     SANLPLRIQGLARMKALEVAEALRRHVPSHPAVAEVRVAPPGFLNFYLDATWLASQVT
                     AIAAEGAAYAGSSSGAGQRLQIEYVSANPTGPVHVGNGRGAAIGSTLARVLRAAGYEV
                     EEEYYVNDAGTQVAVFARTLYARYQQLFGRTVEIPENGYPGEYMVQIAEQIRERHADR
                     FLRPVGEEASPELGELGIQLMVDQIRDDLELLRVQYDRWFSERSLQTEGGPYSEVMRI
                     LEERGHIVRREGAVWFASSQLGESKDNVLIRSDGVPTYFASDVAYHYDKFVLRGFDRV
                     IDIWGADHQGHVSRVKAAVTAVGGDGDHLDVLLYQLVTLKRGTEVVPLSKRAGEIVTL
                     RDVVSEVGADAARFFFLSPSAGSTMDFDLELATRQSDENPVYYVQYAHARISSILSRA
                     ASEELSSEGAETSLLTHDAEQALLRKLLLLPEVVEQVVSSLETHHLPAYAQSLAQSFH
                     VFYTQCRVITDDVPLTKARLALLEATRTVLARTLELMSISAPEQM"
     gene            complement(2722..>3821)
                     /locus_tag="PLX85_03675"
     CDS             complement(2722..>3821)
                     /locus_tag="PLX85_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRC62306.1"
                     /translation="ATLLGGAAGVQAADRAGAITLRTKVALGGLIGALVGLLNVFIVS
                     QLMFVSTSHDLRLLISLIAVSATISLGFSTWVARTITRRLREVTDGVRVLSLGRYETR
                     LHPVGRDEVARLAEDVNVLAGRLQEAEDARAALERDRRDFTIAISHDLRTPLASMRAM
                     AEALDDEVVTTEDEVRRYHRAMRRESERLGAMIDDLFQLSQLDAGSVQLNRRPLALDE
                     IAAEVVDAMQAQAASRGIRLTLEAHGPVPLASLDGALIERAVRNLVSNALAYTPSEGS
                     VAVLVEAAPGGVALHVRDTGQGIASEDVPRVWDRFFRAERSRTRTAEGSDGAGLGLAI
                     VRGIAEAHSGSTAVESTPGVGSTFSLSLPVA"
BASE COUNT          606 a         1196 c         1440 g          579 t
ORIGIN      
        1 gcgcgggccc tcgaacatcg agggccctcg gagtgtcgat cagccgggga taccggtcgg
       61 gccggtagga ccgctttcac ccaccgggcc gctcgggccc gtcacgccga ctggtccgct
      121 cgcgccggtg gagccgatcg ggccgctcgc acctgtgggg cccgtcatgc cgatggggcc
      181 gagcgggcca ctcggacctg tcgggcccgt gggaccggtc ggacccgtgt cgcccttcgg
      241 gcctgagggg ccggcaggac cctgctggtt caacgtgatc gggtactcga aggcagtgca
      301 ggggatgtgg ccgacgacgc ggatgatgcc cgtgtagcgg ttggcacagg cctcgatctg
      361 cgcggtgttg gtcgacgtgg actgcgcccg aacgaggttg aagccgccaa agacagccga
      421 ggcgatgagc accccgacgg cgataccaga caccggtgac cgccacacgc ggttccagat
      481 catttcttac agtctcctcg cgggatggga tcccaccgct catccgctcc aggggcgccg
      541 tggggccgcg gacggcccac gcgattagat gacctgcgaa gcgtcatcga cgcggaagcc
      601 gacaccgagc gtgacctggt accaggcaac gcccttgttt tcgatgcggc cccggatctc
      661 gcggacctgg aaccagtcga ggctgtcgac ggcctcgccg gcggccttga tgccattgtc
      721 gatggctgcc tcgatgccgt cgggcgaggt gcccaccagc tcgacgatgc gatagctgcg
      781 tccttcgtca cccgccatgt cgtattcccc ttcctcctcg ggcggtggcg cccggtgtcg
      841 atgctttctc ggagagtagc gcgtcgtgga acggccaagc tagctcgaga ggcgcgtgca
      901 caccgcggcc tgaccgtgcg cgcaggctcg cgatacactg gcggcacatc gacccctcga
      961 cggtccgcaa gcgcgggccg ttgtgatctg aggccgcata catgatccga gacgacgtgc
     1021 agcgactggt ggtggaggcg ctcacggcag cgcaacaggc gggtgagatc ccgctcttcg
     1081 caccacccga gatccaggtc gagcacccgg cgcgagcgga gcacggcgac tactcggcga
     1141 atctgccgct gcgaatccag ggactcgcgc gaatgaaagc gctcgaggtc gcggaggcgc
     1201 tgcgccgcca tgtgccctcg cacccggcgg tggcggaggt gcgggtcgcg ccgccaggtt
     1261 tcctgaactt ctacctcgac gctacgtggc tggcctcgca agtcacggcg attgcggcgg
     1321 agggcgcggc atacgcgggg agctcaagcg gcgcggggca gcgtctgcag atcgaatatg
     1381 tctcggccaa cccgacaggg ccggtgcacg tcgggaacgg gcgtggggcg gcgatcgggt
     1441 cgacgctggc gcgcgtcctg cgcgcggccg ggtacgaggt cgaggaagag tactacgtca
     1501 acgacgcggg gacgcaggtc gccgtgttcg cgcgaacact gtacgcgcgg taccagcagc
     1561 tcttcgggcg gacggtcgag atcccggaga acggctatcc gggggagtac atggtgcaga
     1621 tcgcggagca gatccgcgag cggcacgccg accgcttcct gcgacccgtg ggcgaagagg
     1681 cgagtccaga actcggcgag ctcgggatcc agttgatggt cgaccagatt cgcgacgacc
     1741 tcgagctgct gcgtgtgcag tacgaccgct ggttctcgga gcgatcactg cagacggagg
     1801 gcgggccgta ctccgaggtg atgcgcatcc tcgaggagcg cggacacatc gtgcggcgcg
     1861 agggcgcggt gtggtttgcc tcctcgcaac tgggcgagtc gaaggacaac gtgctgatcc
     1921 gctcggacgg ggtgccgacg tatttcgcct cggacgtggc ttaccactac gacaagttcg
     1981 tgctgcgcgg gttcgaccgt gtgatcgaca tctggggggc ggaccaccag ggccacgtgt
     2041 ctcgagtgaa ggcggcggtg acggcggtcg gcggggacgg cgaccacctc gacgtgctgc
     2101 tctatcagct cgtgacgctg aagcggggca ccgaggtcgt gccgctgtcg aagcgggcgg
     2161 gcgagatcgt gacgctgcgc gacgtggtgt cggaggtcgg cgcggacgcg gcgcggttct
     2221 tcttcctgtc accctcggcg ggttcgacga tggacttcga cctggagctg gcgacgcggc
     2281 agagcgacga gaaccccgtc tactacgtgc agtacgcgca cgcgcgcatc tcgagcatcc
     2341 tctcgcgggc cgcatccgag gagttgagct cggagggtgc ggagacctcg ttgctcacgc
     2401 atgacgcgga acaggcgctg ctgcggaagc tgctgctgct gccggaggtg gtggagcagg
     2461 tggtgagctc gctcgagacg catcacctac cggcatatgc gcagtctctg gcgcagtcgt
     2521 tccacgtgtt ctatacgcaa tgccgggtga tcacggacga cgtgccgctg acgaaggcgc
     2581 ggctcgcgct cctcgaggcg acgcgcacgg tgctggcgcg gacgctggag ctgatgtcga
     2641 tcagcgcgcc ggagcagatg tagcccacct gcgcgcggtc cgcgactcat cgaaaccatg
     2701 gggcgaacga ttcgggcgtc ttcaggctac ggggagactc agagagaagg tggagcccac
     2761 gccgggggtg gactccacgg cggtggaacc gctgtgcgct tcggcgatgc cacggacgat
     2821 cgcgaggccg aggcccgcac cgtctgaacc ctcggcggtg cgtgtgcggg agcgttcggc
     2881 acggaagaag cggtcccaga cgcgtggcac gtcctcagag gcgatgccct ggccagtgtc
     2941 gcggacgtgc agcgcgacgc cgccgggggc cgcctccacg aggacggcca cggagccctc
     3001 ggacggggtg taggcaaggg cgttactgac gaggttgcgg acggcacgct cgatcagggc
     3061 accatcgagc gacgcgagcg ggacggggcc gtgcgcctcg agcgtgaggc ggatgccccg
     3121 ggaggcggcc tgagcctgca tggcgtcgac cacttcggcg gcgatctcgt cgagggcgag
     3181 cgggcggcgg ttcagctgga cggagccggc atcgagctgg gagagctgga agaggtcgtc
     3241 gatcatggcg ccgaggcgtt cggactcgcg gcgcatggcg cggtggtagc ggcggacttc
     3301 gtcctccgtg gtgacgacct catcgtcgag ggcctcggcc atggcgcgca tgctcgcgag
     3361 cggcgtacgc aggtcgtgtg agatggcgat ggtgaagtca cggcggtcgc gctcgagggc
     3421 ggcgcgcgcg tcctcggcct cctggaggcg gccagcgagg acgttgacgt cctcggcaag
     3481 acgggcgacc tcgtcgcggc cgacggggtg cagacgggtc tcatagcggc caagggaaag
     3541 cacgcgcacg ccgtcggtga cctcgcgcag gcggcgcgtg atcgtcctcg cgacccaggt
     3601 actgaagccg agggagatcg tggcactcac ggcgatcagc gagatgagca ggcggaggtc
     3661 atggctcgtt gagacgaaca tgagctggct gacgatgaag acgttgagca gcccgacgag
     3721 cgcgccgatg agtccgccga gcgcgacctt ggtgcgcaag gtgatggcgc cggcgcggtc
     3781 ggcggcctgt acgcccgcgg cgcctccgag caatgtggcc g
//
LOCUS       DAORPG010000117         3805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2004_length_3805_cov_3.088000, whole genome shotgun sequence.
ACCESSION   DAORPG010000117 DAORPG010000000
VERSION     DAORPG010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2004_length_3805_cov_3.088000"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PLX85_03680"
     CDS             <1..379
                     /locus_tag="PLX85_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62307.1"
                     /translation="VTRYVVGYDQGADSYRLRVTTALPTDEMQIWVPVRFVNGVQAGA
                     GAIMGANRTLQGEQWRVVQRLDAANEGDSIVASLSGLTRANESNPFLGFPGAMAASAV
                     ALTAVIVGVVVLSRVRSGLRGLA"
     gene            376..1053
                     /locus_tag="PLX85_03685"
     CDS             376..1053
                     /locus_tag="PLX85_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC62308.1"
                     /translation="MTATFAIEVTRLSKRYGTTTVLDGVELSLVVGSRTALTGANGAG
                     KSTLLAILSTLVSPTGGQVRIAGYDLQTQAVEVRRRIGVLGHRPMLYEDLTPLENLLF
                     FAKLYGVANPRPRAEAFLEVLGLWSRRFEPVSVLSRGYHQRLAIGRALIHSPEILLLD
                     EPETGLDAEGIELLDALMLREPAFTVLAATHRLDRIAGWADSTANIERGRLVSHQVSA
                     EALAASR"
     gene            1071..1706
                     /locus_tag="PLX85_03690"
     CDS             1071..1706
                     /locus_tag="PLX85_03690"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HRC62309.1"
                     /translation="MRKDLVLLWRHRSGWLSAATFATMSVLTYSFAFDLVTDDVRPLL
                     PGVLWVTFLFAGIIACSRSFADEVEQGTFDAMLVAPIPRSSLYLGKAVSNLAALLTIE
                     VAVMLMATILFNTTLLTAELAFIVGVGTVGYVSLTTLLATLGSRVRSREVLLPVLALP
                     LLVPLLIAGVRATGGALDVPNGDGPWLLLIVVFTIWSAVGSALLFPMVVEQ"
     gene            1703..2431
                     /gene="ccsA"
                     /locus_tag="PLX85_03695"
     CDS             1703..2431
                     /gene="ccsA"
                     /locus_tag="PLX85_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRC62310.1"
                     /translation="MNSVARTATRVYGWRWIVLPPTVGVAMLAMIVGAMVVAPREAVE
                     GEVQRIFYIHVPSAIAAYLAFFIVFVSSIAVLWRRDMRFDVVARAAAGVGVLFTGLVL
                     ATGAIWGHPIWGTWWAWDARLTSTLILFLIFSGYLLARSLSDTSDEQSARYGAVFAII
                     GFLDIPIINMSVRWWRTLHPQPIVLKMPGEQALPGSMLAVLWLGMVAILLLALWFIVL
                     RSETERIATRVNELRVALDRDDEG"
     gene            2435..2665
                     /locus_tag="PLX85_03700"
     CDS             2435..2665
                     /locus_tag="PLX85_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HRC62311.1"
                     /translation="MDHLEYLFAGFAVFWAGLFVYLLWLQARLRTVSRELKQLEERLA
                     EREGGATTPIRGAQASRPQVSLRDIAPQRTTE"
     gene            2686..3648
                     /locus_tag="PLX85_03705"
     CDS             2686..3648
                     /locus_tag="PLX85_03705"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRC62312.1"
                     /translation="MNRPSNKYFALAATTVVATLILIAIGSIVRTTGSGLGCPDWPLC
                     HGQLHPPLEKTAIIEYSHRTAAAIVGVLVVATAVVTLRTRWTDLTSRWLAIASLPLLG
                     FQAWLGKETVERELPPEVVTFHLATAMILLAILTMLAGFAFLGTDRTLLTDGARGRAL
                     RVAAIGAAVTFVVLLSGAYVVGANASNACTSWPGCSEAAVPFADGAREQSIHWLHRFV
                     VAGGLAAVVSVAWALLKLEGAGMLLRRAAWLLVALYAGQILVGASNMWTDFSEASRVA
                     HLALGSAIWALMVLIAVAGRYRAGARGGARMESQPSRSEAAASV"
BASE COUNT          551 a         1201 c         1338 g          715 t
ORIGIN      
        1 cgtcactcgc tatgtcgtcg gctacgatca gggggccgac agctaccggt tgcgggtgac
       61 gaccgcgttg cccaccgatg agatgcagat ctgggtgcca gtgcgcttcg tgaacggggt
      121 gcaagccggc gccggcgcaa tcatgggagc caaccgcaca ctgcagggag agcagtggcg
      181 agtggtgcag cggctggacg ccgccaacga gggggacagc atcgtcgcat cgttgagtgg
      241 gcttacgcgc gcaaacgagt ccaatccgtt cctcggcttc ccgggcgcga tggcggcgtc
      301 cgccgtggcg ctcactgccg tgatcgtagg agtcgtggtg ctgagtcggg tgcgttcggg
      361 actccgaggg ctcgcatgac cgcgaccttc gcgatcgaag tgacccgctt gagcaagcgt
      421 tacgggacca cgaccgtcct cgacggggtc gagttaagcc ttgtggtggg atcgcggacg
      481 gcgctcacgg gagcgaacgg cgctgggaag agcaccctgc tcgccattct ttcgacgctt
      541 gtgtccccga ccggggggca ggtccgcatt gccggctatg acctgcagac gcaggccgtg
      601 gaggtgcgcc ggcggatcgg cgtcctcggg caccggccga tgctgtacga ggacctcacc
      661 cccctcgaga acctgctgtt ctttgcgaag ctgtatggcg tcgcgaaccc gcggccgcga
      721 gcggaagcgt tcctcgaagt gctaggactg tggtcgcggc gattcgagcc tgtctccgtg
      781 ctctcgcgcg ggtaccacca gcgcctcgcc atcggacgag cgctgatcca cagccccgag
      841 atcctgctgc tcgatgagcc ggaaaccggg ctcgatgcgg agggcatcga attgcttgat
      901 gcgctcatgc tgcgcgagcc ggcgttcacg gtgctggcag cgacgcaccg cctggaccgc
      961 atcgcagggt gggccgactc gacagccaac atcgaacgag ggcggctcgt ttcgcatcag
     1021 gtatccgcgg aagcgctggc cgcttcccgg tgaacggggc cctgaccgta atgcggaagg
     1081 atctcgtgct gctgtggcga caccgcagcg gctggctgtc ggcagccacg ttcgcgacga
     1141 tgagcgtgct cacctactcg ttcgcgttcg acctcgtcac cgatgacgtg cgcccgctgc
     1201 tgccgggggt gctgtgggtg acgttcctgt tcgcgggcat catcgcatgc agccgctcat
     1261 ttgccgacga agtagagcag gggacgttcg acgccatgct cgtcgctccg atccctcgga
     1321 gctcgctcta cctcgggaaa gcggtctcga atctggccgc gctactcacg atcgaggtgg
     1381 cggtcatgct gatggcgact atcctgttca atacgacact gctgactgcg gaattggcgt
     1441 tcatcgtggg cgtcgggacg gtcgggtacg tgagtctcac caccctgctc gcgacactcg
     1501 gatcgagagt acggtcgcgg gaggtgttgc tccctgtgct cgcgttaccg ctgctcgtgc
     1561 ccttgctgat cgcgggcgtg cgagcaacgg gcggagcgct cgacgtcccc aacggggacg
     1621 ggccgtggct gctccttatc gtcgtgttca cgatctggag cgcggtcggc agcgcgctgc
     1681 tattccccat ggtcgtcgaa caatgaacag cgttgcgcgc acggcaactc gtgtttacgg
     1741 ctggcgctgg atcgtgctcc cgcccacggt gggcgtcgcg atgctggcca tgatcgtcgg
     1801 cgcgatggtg gtggcgcctc gtgaggccgt cgagggcgag gtgcagcgca tcttctacat
     1861 ccacgtgccg tccgcgattg ccgcgtacct tgcgttcttc atcgtcttcg tgtcctcgat
     1921 cgcagtgctg tggcggcggg acatgcgatt cgacgtggtc gcgcgtgccg cagccggggt
     1981 gggcgtgctc ttcaccggcc tcgtgctggc gacgggggcg atctggggac atccgatctg
     2041 ggggacgtgg tgggcatggg atgcgcgcct gaccagcacc ctgatcctgt tcctgatctt
     2101 ctcggggtac ctgctggcga ggagtctctc ggatacgtcc gacgagcaat ccgcccggta
     2161 cggcgcggtg ttcgccatca tcggattcct cgatattccg atcatcaata tgtccgtgcg
     2221 ctggtggcgg acgttgcatc cccaaccgat cgtgctgaag atgccgggcg agcaggcgct
     2281 gccagggtcg atgctggccg tgctgtggct cggcatggtc gcgatcctgc tactcgcgct
     2341 ctggttcatc gtacttcgct ccgaaaccga gcgaatcgct acacgggtca atgagctgcg
     2401 tgtcgcactc gaccgcgacg acgaggggta agccatggac cacctcgagt acctgtttgc
     2461 tggcttcgcg gtcttctggg ccggactgtt cgtgtacctg ctctggctcc aggcccggtt
     2521 gcgcaccgtt tcgcgcgaac tcaagcagct ggaggagcgg ctggccgaac gcgagggcgg
     2581 ggcgacgacg cctatccgag gtgcgcaagc gtcccggccc caggtctcgc tgcgagacat
     2641 cgctccgcag cgcacaactg agtgacgtcg tagcatggtg aaaccgtgaa cagaccgtcg
     2701 aacaagtact tcgcgctggc cgcgacgacg gtcgttgcca ccctgatcct gattgcgatc
     2761 ggctcgatcg tgcgtacgac gggatcgggg ctcggctgtc cagactggcc gctgtgccac
     2821 gggcaattgc atcctcccct cgagaagacg gcgatcatcg agtacagcca ccgcacggcg
     2881 gcggcgattg tgggtgtcct ggttgtcgcg acggcggtcg tgacgctgcg cacacgctgg
     2941 acggacctca cgagccgatg gctggctatc gcctcgctgc cgctgctggg gttccaggcc
     3001 tggctcggca aagagaccgt ggaacgtgag ctaccgcccg aggtggtcac gttccacctc
     3061 gcgacggcca tgatcctgct ggcgattctc acgatgctgg ccggtttcgc attcctcggc
     3121 accgaccgga ctttgctcac ggatggggcc cgcggccggg cgttgcgtgt ggcggcgatt
     3181 ggggcggcgg tcacgtttgt cgtgctgctg agcggggcct atgtcgtcgg cgccaacgcg
     3241 agcaatgcgt gcacgagctg gccggggtgt tccgaggcgg cggtgccctt cgcggacggc
     3301 gctcgggaac agtcgatcca ctggctgcac cggttcgttg ttgcgggcgg cctcgccgcc
     3361 gtcgtttccg tcgcgtgggc gctgctcaag ctcgaggggg ccgggatgct gctcaggcgg
     3421 gcagcctggc tgctcgtcgc gctctacgca gggcagattc tggtcggggc gtccaacatg
     3481 tggaccgact tctcggaggc gtcgcgagtg gcccacctgg ccctcgggtc ggcgatctgg
     3541 gcgctgatgg tgctgatcgc ggtggccgga cgataccggg cgggggcccg tggcggcgcc
     3601 aggatggaat cgcagccgtc gcgatccgag gccgcggcga gtgtctagcg ccgccatgga
     3661 ggcgggcgac ctcgccgagg tccagccggg ggcgacgcgc agccgaatct cgcgcacggt
     3721 ccgcacgtac atcgcgctga cgaagcccca catcatctgg ctgctgctga tcaccaccgt
     3781 cccggcgatg gtgctcgccg agcag
//
LOCUS       DAORPG010000118         3801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2012_length_3801_cov_3.573412, whole genome shotgun sequence.
ACCESSION   DAORPG010000118 DAORPG010000000
VERSION     DAORPG010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2012_length_3801_cov_3.573412"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1017
                     /gene="murI"
                     /locus_tag="PLX85_03710"
     CDS             157..1017
                     /gene="murI"
                     /locus_tag="PLX85_03710"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRC62313.1"
                     /translation="MHTAVGVFDSGVGGLTVVAALRRLNPELPIRYIADGAWFPYGER
                     SPEEIEERSLLLADALIAEGCAPIVVACNTASSSALDRLRERFEVPIIGMEPPLKPAV
                     ERTRTGKVAVLATPSTARGERLARLQDAYGGGVEVTTVPMPGLADLVESGHVAGERVE
                     QLLHRTLGPLADSGIDEVALGCTHYGFLRATFDSMLDELLGTDVDVIDPAEPVARRAL
                     QQVEAHGIAVPPVERGAPVIAATTGDPARFAEAVARLRADGADLPVLDILPLPLALPA
                     PAGLPGERGR"
     gene            1014..1937
                     /gene="pyrF"
                     /locus_tag="PLX85_03715"
     CDS             1014..1937
                     /gene="pyrF"
                     /locus_tag="PLX85_03715"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRC62314.1"
                     /translation="MTAATFRAKYEAAARANESLLCVGLDPDPALMPPGITTLEFLRG
                     IIEATADLVCCYKPNAAFFEREGLPGWESLQQVIEAIPDEIPVILDAKRGDVGHTSEA
                     YARAVFDALGADAVTANPYLGGDGLEPFLAHEDRHTFVLCRTSNAGARDLQDLLVTAL
                     PSEGARSTPNLPPSRGESAVPASTPSQGEGGGGGDSDTPRPLYEHVAMLANSWNTRGN
                     VGLVVGATYPAEAARIRALCPELLFLLPGIGAQQGDVDAAVRASLDARGGGILINSSR
                     GILYAGRDAGFAAAARTEATRLRDAINAARP"
     gene            1964..2227
                     /locus_tag="PLX85_03720"
     CDS             1964..2227
                     /locus_tag="PLX85_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HRC62315.1"
                     /translation="MVLDLRVGDVLRLRRKHPCGGFDWDVVRVGADVGMRCRECGRRV
                     LLDRPTLHRRLKSVLERGVPIDPAIERALFGDLAAPERVEEGS"
     gene            2319..2837
                     /locus_tag="PLX85_03725"
     CDS             2319..2837
                     /locus_tag="PLX85_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRC62316.1"
                     /translation="MIELTIESIRVSLRNYQRVVILREKDADRYLPIWIGPAEADAIA
                     VRLQDVAVARPLTHDLLTSMIENLGGVVRAVVVNDLANDTFFARIHVEQAGQIIEVDS
                     RPSDAIALAVRTGVPIYADESVLDRAGVLLNAEGEPLEAAEGAGGITPEERAKLSAFS
                     EFIAELDLDDFD"
     gene            2906..3184
                     /locus_tag="PLX85_03730"
     CDS             2906..3184
                     /locus_tag="PLX85_03730"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRC62317.1"
                     /translation="MLESPRFQSAGRWTIWNSPAFGLTGIGFYLATCIVGLTLVGHLL
                     DNRFDTQPVLTLVFLVLGLLAGFYGAYQQLKELLAHIERQRGSKGRRG"
     gene            3181..>3801
                     /locus_tag="PLX85_03735"
     CDS             3181..>3801
                     /locus_tag="PLX85_03735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRC62318.1"
                     /translation="MKLVIAIVVIALMAVGFLFVKGPSPAIVVAPEHIFNLGPIEVTN
                     TMFTSWWVVGILCLIAVFAGPRMSVVPSGFSGVVEGALSAFYGIVVQVAGEQNARRFF
                     WVVSTIFFYVLMSNYFGLLPMNNVIGKPEAGHGEKQVIFQTTSIAGLDLAYIKVNPDE
                     CEVGDPEGCPTAAEHSAALGAPSSASVSAQPGGAPAVTAASEEAGAK"
BASE COUNT          555 a         1433 c         1168 g          645 t
ORIGIN      
        1 ggcgcggtac ggccggcacg ttccacctcc cgaggccgtc cagtcgctcg agcgtcgcct
       61 cgcagactgg gaacagcagc acccggcccc ccatccctga ggcgggttac gtggcggttg
      121 ctatagtgaa ggcgaacgat cagtaagggg ccgcccgtgc acactgccgt tggcgtcttc
      181 gactctggcg tgggcgggct caccgtcgtc gccgcgctcc gccgcctcaa cccagagctc
      241 cccatccgct acatcgccga cggcgcctgg ttcccctatg gcgagcgttc tcccgaggag
      301 atcgaggagc ggtcgctcct cctcgccgat gcgctcatcg ccgagggttg cgcgcccatc
      361 gtcgtcgcct gcaacaccgc ctcctcgtcc gcgctcgatc gtctgcgcga gcgcttcgag
      421 gtcccgatca tcggcatgga gcctccgctg aagcccgctg tcgagcgcac gaggacgggc
      481 aaggtcgctg tgctcgcgac tccttcgacc gcccgcggcg aacgcctcgc ccgcctgcag
      541 gacgcctacg gcggcggcgt cgaggtcacc accgtcccaa tgccgggcct cgctgacctg
      601 gtggaatccg gccacgtcgc gggcgaacgc gtcgagcagt tgctccaccg caccctcgga
      661 ccgcttgccg attccggcat cgacgaggtc gccctcggct gcacgcatta cggcttcctg
      721 cgcgcgacct tcgactcgat gctcgacgaa ctgctcggca ccgatgtcga cgtgatcgac
      781 cccgccgagc ccgtcgcccg ccgcgccctc cagcaggtcg aggctcacgg catcgccgta
      841 ccccctgtcg aacgaggcgc tccggtgatc gccgcgacaa ccggagatcc ggcccgcttc
      901 gccgaagccg tcgcgcgcct tcgcgccgac ggtgccgacc tcccggtcct cgatatcctg
      961 cctctgccgc tcgcgctgcc cgcgcccgcg gggctcccgg gagaacgcgg ccggtgaccg
     1021 ctgccacctt ccgcgcgaag tacgaggcgg ccgctcgcgc gaacgagtcg ctgctgtgcg
     1081 tgggcctcga ccccgacccg gccctgatgc cccccggcat cacgacactc gaattcctgc
     1141 gcggcatcat cgaggcgacc gcggacctcg tctgctgcta caagcccaat gccgccttct
     1201 tcgagcgcga gggcctgccg ggctgggagt cgctccaaca ggtcatcgag gcgattccgg
     1261 acgagattcc agtcatcctc gatgccaagc gcggcgatgt cggccacacg tccgaggcct
     1321 atgctcgcgc cgtcttcgac gccctcggcg ccgacgccgt gaccgccaac ccctacctcg
     1381 gcggcgatgg cctcgaaccc ttcctggctc acgaggatcg ccacaccttc gtgctttgtc
     1441 gcacctcgaa cgccggagcc cgcgacctcc aggacttgct ggttacggct ctcccctccg
     1501 agggtgcgcg gtcaactccg aatctccctc cgagccgggg cgaatccgcg gttcccgcct
     1561 ccaccccctc gcagggggag ggcgggggcg gcggtgattc cgacacccct cgtccgctct
     1621 acgaacacgt cgcgatgctc gccaacagct ggaacacgcg cggcaacgtc ggcctggtcg
     1681 tcggcgccac gtatcccgcc gaggcggctc gcattcgcgc gctctgcccc gagctgctgt
     1741 tcctgcttcc cggcattggc gcccagcagg gcgacgtcga cgccgcagtt cgcgccagcc
     1801 ttgatgctcg aggcggcggc atcctgatca actcctcccg gggcatcctt tacgccggcc
     1861 gcgacgccgg cttcgccgcc gccgctcgca ctgaagccac tcgcctgcgc gacgccatca
     1921 acgccgcgcg gccctgactc cgcgctgcga gtaagatcga ggcatggtcc tcgatctccg
     1981 cgtcggcgat gtcctgcgcc tccgccgtaa gcacccctgc ggcggattcg attgggacgt
     2041 cgtgcgcgtc ggggccgacg tcggcatgcg ctgccgcgaa tgtggccgac gagtgctcct
     2101 cgaccgcccc acgcttcatc gccggctgaa gtcggtcctc gagcgaggcg tccccatcga
     2161 ccccgcgatc gagcgcgcgc tcttcggcga tctcgccgcc cccgagcggg tcgaggaggg
     2221 ctcgtgagcg caacgccgcc gctcgtcgcc gtttcgttga ctgccttcga ggcccgctcc
     2281 tacactcgga tcgtgcggca ctgcaaggcg caagcctcgt gatcgaactc accatcgaga
     2341 gcattcgcgt cagcctgcgc aactatcagc gggtcgtcat ccttcgcgag aaggacgccg
     2401 accgctacct gccgatctgg atcgggcccg ccgaggcgga cgccatcgcc gtccggctcc
     2461 aggatgtcgc ggtcgcccgc cccctgaccc acgacctgct gacgagcatg atcgagaacc
     2521 tcggcggcgt ggtgcgtgct gtcgtcgtca acgatctcgc caacgacacc ttcttcgctc
     2581 gcatccatgt cgagcaggcc ggccagatca tcgaggtcga ctcgcgcccc tcagacgcga
     2641 tcgcgctggc ggtccgcacc ggcgtgccga tctacgccga cgagtccgtg ctcgaccgcg
     2701 ctggcgtcct cctcaacgcc gagggcgaac cccttgaggc agccgagggc gcgggcggca
     2761 ttacgccaga ggagcgcgcc aagctctcag ccttcagcga attcatcgcc gaactcgatc
     2821 tcgacgactt cgactaacgg gagtagtaac ccggcgatcg ctaattagtg cagtgggtca
     2881 caagtgttga tccaccgatc cgcccatgct agagtccccc cggttccaat ccgcagggag
     2941 atggaccatc tggaactcac cggcgttcgg gctgaccggc atcggcttct acctggcgac
     3001 atgtatcgtc gggctgacgc tcgtaggcca tctgctcgat aaccggtttg acacgcagcc
     3061 ggtactcacg ctcgtgttcc tggtgctcgg tctgctggct ggcttctacg gagcctacca
     3121 gcaactcaaa gagctactgg cgcacatcga gcggcaacga ggctcgaaag gccgtcgagg
     3181 atgaagctgg tcatcgccat cgtcgtcatc gccctgatgg ccgttggctt cctcttcgtc
     3241 aaaggtccct ccccggcgat cgtcgtcgcc cctgagcaca tctttaacct cggccccatc
     3301 gaggtgacga acacgatgtt cacctcgtgg tgggtggtcg gcatcctctg cctgatcgcc
     3361 gtcttcgccg ggccgcgtat gtccgtcgtg ccgtccggct tcagcggcgt ggtcgagggc
     3421 gcgctgagtg ccttctacgg catcgtcgtc caggtcgccg gcgaacagaa cgcccgccgc
     3481 ttcttctggg tcgtctcgac catcttcttc tacgtgctga tgtccaacta cttcggcctc
     3541 ctgccgatga acaacgtcat cggcaagccc gaggccggac acggcgagaa gcaggtcatc
     3601 ttccagacca cgtcgatcgc cggtctcgac ctcgcctaca tcaaggtcaa cccggacgag
     3661 tgtgaagtcg gcgacccgga gggttgcccc acggcggccg agcacagcgc cgccctcggt
     3721 gcgccgtcct ccgccagcgt ttcggctcag cccggcggcg ccccggctgt caccgccgcc
     3781 tccgaggagg ccggcgcgaa g
//
LOCUS       DAORPG010000119         3779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2039_length_3779_cov_3.136681, whole genome shotgun sequence.
ACCESSION   DAORPG010000119 DAORPG010000000
VERSION     DAORPG010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2039_length_3779_cov_3.136681"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PLX85_03740"
     CDS             <1..543
                     /locus_tag="PLX85_03740"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRC62319.1"
                     /translation="KGIADRYAAEGYLALAPDMYHGVVTDEPDEARKLVMALERDEAA
                     KEIDAAVAWLKATQGVAKVGCVGFCMGGGLTLATALRPNSGIDAAHVYYGGGMPDDAT
                     LATIRVPVMGSYGADDAGIPADRVDALRNALASSGVDHDIKLYEATGHSFFNSGHSLH
                     PQSAADSWERSKAWFAKHLA"
     gene            677..1411
                     /locus_tag="PLX85_03745"
     CDS             677..1411
                     /locus_tag="PLX85_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC62320.1"
                     /translation="MTEDSMAASAGQPIAARPPLISLVGVHKTYRTGRLEYPAVRGVD
                     LTIQQGEMVSIVGPSGSGKSTILNLVTGIDHPTAGEVHVDGQRIDQLSEEELARWRGR
                     RVGIVFQFFQLLPTLTALENAELPMDFARLWNGRERTARATHHLDLVGLGDKLHHLPG
                     ELSGGEQQRVAIARALACDPVLLVGDEPTGNLDTETAARMFDLLAGLNEGGMTVVYVT
                     HDAHLASRAPRSIVIRDGRIDDDRTS"
     gene            1408..3762
                     /locus_tag="PLX85_03750"
     CDS             1408..3762
                     /locus_tag="PLX85_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRC62321.1"
                     /translation="MSALTRKSWKDVRRRWARSLFTIATIAVAVAGLSLFSLMHLLNE
                     AMDRRVVDDRLHDVQVYVQDAGLSPAELDQLAALPGVIALDARTAYVTRLREGDRRVD
                     TLLVGVPDFAAQNVDVVHLESGSLPGASEALTDTQNARSGRFKGGAGTSIQVEDDAGR
                     LREVTISGAGNTLEYTVIATEDRAVLYAPQRLVNEIAGASGVNRLDFRLADPAQAQAA
                     LAAIQGWFAAHKPGAEFTDLPDVRSVGTWPGKENSENFATLFYVGGILALLSATALVS
                     NTVTTMVAEQRREIAVMKAIGGSRRQIGLSLFRTVWILAAIGTVIGVLLGIPFSSFLA
                     SFVGKQFLGVQTQWGYSLPVVIFSIVVGLLGPTLAAIPGLLHATRITVREGFEGALTA
                     SQRSAADRLLQRVPLPRTAQVGLRNITRRKVRTVGTTLQVGLAVGVALGFLALGQTVG
                     TVTARTWDVMHWDVIVSQRSTVALDERAVSLIRGVEGAQGGHPVLYNTVEIAGTRYEA
                     WGLPVDTALYEPDIVSGRWFDSTDATAPSVVLGRAIAAKVGLHVGDTVDVGTASGVVP
                     LQVVGLDGRLINNSTSVFIPLAEFQRLLGRDDTNAYWLLSASKENPAIDRLAASAEDT
                     LASGGYPADTEIRYVEKDANLASNRILVSVLAVMGVPIVAIGLIGLVNMMTMNVLERG
                     REIGILRCIGARGRDILRIFQTEALSVALLGWVLSIPAGWLIGWSLVRVIATMFKFGS
                     IPYSFPLWYPVLALPATLFLAYLVVIAPVRRAARVRPGDALRYE"
BASE COUNT          557 a         1446 c         1203 g          573 t
ORIGIN      
        1 aaaggcatcg ccgaccgcta cgccgccgag ggctacctcg ccctcgcgcc cgacatgtac
       61 cacggcgtcg tcaccgacga gcccgacgag gcgcgcaagc tcgtcatggc cctcgagcgc
      121 gacgaggcgg cgaaggagat cgacgcggcg gtcgcttggc tgaaggccac gcagggcgtc
      181 gcgaaggtcg ggtgcgtcgg cttctgcatg ggtggcggcc tcaccctcgc caccgccctg
      241 cggccgaatt ccggcatcga cgcagcacac gtctactacg gcggcggcat gcccgacgac
      301 gcgacgctgg ccaccatccg ggtccccgtc atgggttcct atggcgcgga cgacgccggc
      361 atccccgcag accgcgtgga cgccctccgc aacgccctcg cctcgagcgg cgtcgaccac
      421 gacatcaagc tctacgaggc aaccggccac agcttcttca atagcggcca ctcgcttcac
      481 ccgcagtcgg ccgccgactc gtgggagcgc tcgaaggcct ggttcgcgaa gcatctcgcg
      541 tgacgcagac gcagcccggg cggcgaggcg aggcgtccgg tcgacaccga acgaccggcc
      601 cgggcgatga ccttcgcccc tgtacgaggc ggctccaggc gccgatcgtg tgcggcacgg
      661 ctggagggac ggcagcatga ccgaggactc catggcagcc agtgccggcc aacccatcgc
      721 cgctcggccg ccgctgatct cgctcgtcgg cgtccacaag acctatcgca ccggccgcct
      781 cgaatacccc gccgtccgag gcgtcgacct cacgatccag cagggcgaga tggtctccat
      841 cgtcggtccc tccggcagcg gcaagtcgac catcctcaac ctggtcaccg gcatcgacca
      901 cccgacggcg ggcgaggtgc acgtcgacgg tcagcgcatc gaccagctga gcgaggagga
      961 actcgcgcgc tggcgggggc ggcgcgttgg catcgtcttc cagttctttc agctcctgcc
     1021 caccctcacc gccctcgaga acgccgagct gccgatggac ttcgcccgcc tctggaacgg
     1081 ccgggagcgc acggcgagag cgacgcatca cctcgacctc gtcggcctgg gcgacaagct
     1141 gcaccacctt ccgggcgagc tatcgggcgg cgagcagcag cgcgtcgcca tcgcgcgcgc
     1201 cctcgcctgc gaccccgtgc tgctcgtcgg cgacgagccg acaggcaacc tcgacaccga
     1261 gaccgccgcg cgcatgttcg atctcctcgc cggactgaac gagggcggca tgaccgtcgt
     1321 ctacgtgacc cacgacgccc acctcgcctc gcgcgcgccg cgttcgatcg tgatccgcga
     1381 cggccggatc gacgacgatc gcacctcatg agcgccctca cccggaagtc atggaaagac
     1441 gtccggcgtc gctgggcgcg ctcgctcttc accatcgcca cgatcgcggt ggcggtcgcc
     1501 ggcctctcgc tgttctcgct gatgcacctg ctgaacgagg cgatggatag gcgcgtcgtc
     1561 gatgaccgcc tgcacgacgt ccaggtctac gtccaggacg ccggcctcag ccccgccgag
     1621 ctcgaccagc tggcggccct tcccggcgtc atcgcgctcg atgcccgcac cgcctatgtc
     1681 acccgcctgc gcgagggcga ccgccgcgtc gacacgctcc tcgtcggcgt gcccgacttc
     1741 gcggcgcaaa acgtcgatgt cgtccacctc gagtccggct ctctgcccgg agcatccgag
     1801 gcgctgaccg acacccagaa cgcccgcagc gggcggttta agggcggcgc cggcaccagc
     1861 atccaggtcg aggacgacgc cggtcggctg cgcgaggtca cgatctcggg cgccgggaac
     1921 acgctcgagt acacggtaat cgccaccgag gaccgcgcgg tgctctacgc gccccagcgg
     1981 ctcgtcaacg agatcgccgg cgcctcgggc gtgaaccgcc tcgacttccg cctcgccgat
     2041 ccggcacagg cacaggccgc gctcgccgcg atccagggct ggttcgcggc ccacaagccc
     2101 ggcgccgagt tcaccgacct gcccgacgtc cggagcgtcg gcacctggcc cggcaaggag
     2161 aactccgaga acttcgcgac gctgttctat gtcggcggca tcctcgcgct gctctccgcg
     2221 accgcactcg tctccaacac ggtcaccacg atggtcgccg agcagcgtcg cgagatcgcg
     2281 gtgatgaagg cgatcggcgg cagccggcgg cagatcggcc tgtcgttgtt ccggacggtc
     2341 tggatcctcg cggcgatcgg taccgtcatc ggggtcctgc tgggcatccc gttcagcagc
     2401 ttcctcgcga gtttcgtcgg caagcaattc ctgggcgtgc agacgcagtg gggctactcg
     2461 ctgcccgtcg tcatcttcag catcgtcgtt ggcttgctcg gccccacgct cgccgccatc
     2521 cccggcctcc tccacgcgac gaggatcacc gtccgcgagg gcttcgaggg cgccctcacc
     2581 gcctcgcagc gctcagccgc cgaccgcctg ctgcagcggg tcccgctccc gcgcacggcg
     2641 caggtgggcc tccgcaacat cacgcgccgc aaggtgagga cggtcggcac caccttgcag
     2701 gtcgggctgg ccgtcggcgt cgcactcggc ttcctcgcgc tgggccagac cgtgggcacc
     2761 gtgacggcgc gcacgtggga cgtgatgcac tgggacgtca tcgtcagcca gcgctccacg
     2821 gtcgcgctcg atgagcgcgc ggtctcgctc atccgtggcg tcgagggcgc gcagggcggc
     2881 catcccgtcc tctataacac cgtcgagatc gccggcaccc gctacgaggc gtggggcctc
     2941 cccgtcgaca ctgcgctgta cgaaccggac atcgtctcgg gccgctggtt cgattccacc
     3001 gacgcgaccg cgccaagcgt cgtcctcggc cgcgcgatcg ccgccaaggt cggcctccac
     3061 gtgggcgaca ccgtcgatgt cggtaccgcc tcgggcgtcg tccccctgca ggtcgtcggc
     3121 ctcgacggac ggttgatcaa caacagtacg agcgtcttca ttccgctcgc cgagttccag
     3181 cgcctcctcg gccgcgacga caccaacgcc tactggctcc tgtccgccag caaggagaac
     3241 ccggcaatcg accgcctcgc ggccagcgcc gaggacacac tcgcgtccgg cggttatccg
     3301 gcggacaccg agatccgcta cgtcgagaag gacgcgaacc tcgcgtccaa ccgcatcctc
     3361 gtgagcgtcc tcgccgtcat gggcgtcccg atcgtcgcga tcggcctgat cggcctcgtg
     3421 aacatgatga cgatgaacgt cctcgagcgc gggcgggaga tcggcatcct ccgctgcatt
     3481 ggcgcccgcg gcagggacat cctgcgcatc tttcagaccg aggcgctctc ggtcgccctc
     3541 ctcggctggg tgctctccat ccccgccgga tggctgatcg gctggtcgct cgtgcgcgtc
     3601 atcgccacga tgttcaagtt tggctccatc ccctactcat tcccgctctg gtaccccgtg
     3661 ctcgcgctcc ctgcgacgtt gttcctcgcc tacctcgtcg tgatcgcacc tgtgcgccga
     3721 gccgcgcgcg tccgccccgg cgacgccctg cgctacgagt agccgccccc ctggtcgcc
//
LOCUS       DAORPG010000120         3764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2053_length_3764_cov_3.827177, whole genome shotgun sequence.
ACCESSION   DAORPG010000120 DAORPG010000000
VERSION     DAORPG010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2053_length_3764_cov_3.827177"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="PLX85_03755"
     CDS             <1..626
                     /locus_tag="PLX85_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012749617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRC62322.1"
                     /translation="RDEIWCVVDAFAQGARRAVEAGFRAIELHEAHGYLVAQFLSPFS
                     NQRGDDYGGDFEGRTRLAVEVASAVRGAIPGGIPLLVRLSASEYVEGGWDLEQTIELA
                     RRLKAVGVDLIDASSGGNLPHQRVHAHPGYQVPFARAIRERAGMATGAVGLITSATHA
                     DAIVAGGDADAVFVGREALRDPYFGLHAAGQLRSDLDWPVQYQRAKP"
     gene            771..2252
                     /locus_tag="PLX85_03760"
     CDS             771..2252
                     /locus_tag="PLX85_03760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC62323.1"
                     /translation="MTTEAVAAPAQPRADRYLIFGVLAISLLAFVMQFAMVSVSLADL
                     IKDLNAPLRWGGWVLTIFMIGQVIAMPAAGRLSERLGARTVFAGGFGLFAAASLVAAV
                     APNIYVMILARAAQGLAGGGLMPSGMSMIAEAFAEGRQRAIGLFSSILPFGAVAGPGV
                     GGLISEHLGWRWTFAFHVPVGLLVLIAAFTILPRGKRQGGQRVDFLGIAFLAIALSAL
                     IYALTELGLRDADPNMAVVAISLAVSAVTWAALARHELTTEAPLIDLDLLRRREFVAP
                     NAISFFFGFCWVGAFSLLPLYVRSAYDMGPAASGALMAPRAGLMGLTSMAASMLILRT
                     GYRKPIATGLFGLSLVLGLVSLGLHDPTILGVQVDTFWWLFGVVLLGGFFFGMANPSL
                     QNASMDIAPDRIASIAGLRGMFQTVGGTMGISISVLVASRSETTAIGLQHAYRAYSLL
                     MLFVPLFVLAIAETARTRATRTESARPAVPERPAMERAGGDGG"
     gene            2328..3107
                     /locus_tag="PLX85_03765"
     CDS             2328..3107
                     /locus_tag="PLX85_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62324.1"
                     /translation="MPAGPLQRRDAIRHAFRLGSALALAAVVGCDAQASTEPPLTSAT
                     APIASPDASQRAERATSTPARPLPTSVRRDLVPTRLMIWRVGIDAPVTAAEVAPGANG
                     QLEIEVPESGVVTANASLGGHSTNNAWVVGHSRWHRVPQLLYTLPLVQAGDRMELESV
                     DGATGARLPRLTFEVTELVVTDTETTARRVYGPTPDVPRLIVQTSARQTYDPEWILDR
                     ELVLGKSQVDLAGPMDDLSRYLLLLVIGRIEEASLRVLAGG"
     gene            3236..3490
                     /locus_tag="PLX85_03770"
     CDS             3236..3490
                     /locus_tag="PLX85_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62325.1"
                     /translation="MRRILLSGALSGALLVSLLGTASAASPKQTEPGAPGTPNCVGQT
                     MAFLAQNGPDGVHGIGGLVHAFDASVKDLKAFVQLYCAGE"
BASE COUNT          523 a         1279 c         1318 g          644 t
ORIGIN      
        1 cgcgcgacga gatctggtgc gtggtagacg ccttcgcgca gggagcacgc cgcgccgtcg
       61 aggcgggctt ccgtgcgatc gagctgcacg aggcgcacgg ctacctcgtg gcgcagttcc
      121 tctcgccatt ctcgaatcag cgcggcgacg actacggcgg cgacttcgag ggccgcaccc
      181 gactggcagt cgaggtggcc tcggcggtgc gaggagccat tcccggcggc atcccgctgc
      241 tggtgcggct ctccgcgagc gagtacgtcg agggcggctg ggacctggag cagacgatcg
      301 agctggcacg gcgactgaag gcagtcggcg tcgacctgat tgacgcctca tcaggcggga
      361 acctcccgca ccagcgggtg catgcccatc cgggctacca ggtgccattc gcccgagcca
      421 tccgggagcg cgcgggcatg gcgacggggg ccgtcggcct catcacctcg gcaacgcacg
      481 ccgacgccat cgtcgccggc ggcgacgcgg acgccgtctt cgtcgggcgg gaggcgctcc
      541 gtgacccgta cttcgggctt cacgccgcgg gccagctccg gagcgacctc gactggcccg
      601 tacagtacca gcgggcgaag ccctgaaatc ggcgcccctc gacggctcgc cgctatcaca
      661 gtggctgata cgctgtgcca gtcgagccga ccgcgcgacg accagttgaa gcgcaccagc
      721 gtcgggagcg gcgacctcac aggcgaggta cgccgtgacg aggaggcgac atgaccaccg
      781 aggccgtagc ggcgcccgcc caaccacgag cagaccgtta cctcattttc ggggtgctcg
      841 cgatctcgtt gctggcgttc gtcatgcagt tcgccatggt gtcggtgtcg ttggccgacc
      901 tgatcaagga cctcaacgca ccgctgcgct ggggcggctg ggtgctcacg attttcatga
      961 tcgggcaggt catcgccatg cccgcggccg gccggctctc tgagcggctc ggcgcgagga
     1021 cggtgttcgc cggcgggttc gggttgttcg cggccgcttc gctggtggcg gcggtcgcgc
     1081 ccaacatcta cgtcatgatc ctcgcgcgcg cggcgcaggg cctcgcgggt ggcgggctca
     1141 tgccatccgg catgagcatg atcgccgagg ctttcgcgga ggggcgccag cgcgcgatcg
     1201 gcctgttttc ctcgatcctc ccgtttggcg cggtcgccgg gccgggcgtg ggcggcctga
     1261 tcagcgagca cctcgggtgg cgctggacgt tcgcgttcca cgtaccggtc gggctgctgg
     1321 tgctcatcgc cgcgttcacg atcctgcctc gtggcaagcg gcagggcggc cagagagtgg
     1381 acttcctcgg gattgccttc ctggcgatcg cgctgtcggc gctgatctat gcgctcacgg
     1441 aactcggcct gcgcgatgct gacccgaaca tggcggtcgt cgcgatttcc ctcgcggtgt
     1501 cggcggtcac atgggccgcg ctcgcacgcc atgagctgac gaccgaggcc ccgctgatcg
     1561 acctcgacct gctgcgccgc cgcgagttcg tggcgccgaa cgcaatctcg ttcttcttcg
     1621 ggttctgctg ggtcggcgcg ttctcgctgc tgccgctcta tgtgcgcagc gcctacgaca
     1681 tgggcccggc ggcgagtggc gccctaatgg cgccgcgggc cggcctgatg gggctgacct
     1741 cgatggcggc ctcgatgttg atcctgcgca cgggctatcg gaagccaatc gcgaccggac
     1801 tgttcgggct gtcgctcgtg ctcggactcg tgtcgctcgg gctgcacgac ccgaccatcc
     1861 tcggggtgca ggtcgacacc ttctggtggc tgtttggcgt ggtactgctg ggcggcttct
     1921 tcttcgggat ggcgaacccg tccttgcaga acgcgagcat ggacatcgcg ccggaccgca
     1981 tcgcctcgat cgccggactg cgtgggatgt tccagacagt cggcgggacg atgggcatct
     2041 cgatctcagt gctggtggcc tcgaggtctg agacgacggc gatcgggttg cagcacgcct
     2101 atcgcgccta ctcgttgctg atgctcttcg tgccgctgtt cgtgctggcg atcgcggaaa
     2161 ccgcacgcac gagggcgacg aggaccgaaa gcgctcgtcc ggcggtgcct gagcggccgg
     2221 cgatggagcg tgcgggcgga gacggcggct agccgaactc gccttccggg cctccgcgtg
     2281 tatgctggaa gccggcctca ctcccctcga acggacgcct tcctcgcatg ccggctggtc
     2341 cgctgcagcg tcgagatgcg attcgccatg cgttccggct cggctccgcg ctcgcgctgg
     2401 cggccgtcgt cggctgcgac gcgcaggcct cgactgagcc gcctctgacc tcggccaccg
     2461 cccccatcgc gtctccggat gcctcgcagc gtgcggagcg cgcaacgagc acgcccgcac
     2521 gaccgctgcc gacctcggtg cgacgcgacc tggtacccac gcggctgatg atctggcgcg
     2581 tcggcatcga tgccccggtc acggcggccg aggtcgcccc gggcgcgaac ggccagctcg
     2641 aaatcgaggt gccggaatcg ggcgtggtca cggccaacgc ctcgctgggc gggcattcca
     2701 cgaacaacgc atgggtggtc gggcacagcc gctggcaccg cgtgccgcag ctgctgtaca
     2761 cgcttccact ggtgcaagca ggcgaccgca tggaactcga gagcgtggac ggcgcgacgg
     2821 gcgcgcggct gccgcggctg acgttcgagg tcacggagct cgtggtgacg gacacggaga
     2881 cgaccgctcg acgcgtgtat gggccgaccc cggatgtccc ccgcctcatc gtccagacga
     2941 gcgcgcgtca gacgtacgac ccggagtgga tcctcgaccg cgaactggtg ctggggaagt
     3001 cgcaggtcga cctcgcgggg ccgatggacg acctctcgag gtacctgttg ctcctcgtga
     3061 tcgggcggat cgaggaggct tcgctgcggg tgctggctgg aggctgaggc ctcgagagcg
     3121 gcgccgccgc gagtgtcatc actttacaga caaggaatgg cgctcctgta tcatgcgccg
     3181 gctcgtccac gcgcggaccg agtgccagcc gttccagatc tgaaaggacc acctcatgcg
     3241 tcggattctt ctttcaggcg ccttgtctgg agctctgctt gtgtcgctgc tcgggaccgc
     3301 gtccgcggcc tcgccgaagc agaccgagcc gggcgcgccg ggcacgccaa actgcgtggg
     3361 ccagacgatg gccttcctcg cacagaacgg accggacggc gtccacggta tcggcggact
     3421 tgtccatgcc ttcgatgctt ccgtgaagga cctcaaggcg ttcgtccagc tgtactgcgc
     3481 cggcgagtaa cctcacgcct cggccgctga ggccgagttg acccctgccc cggcggcctg
     3541 gcgcgtcagg ccgaccgagg cgagcgccct cgccgccacg tccttcctcg atccgcgcga
     3601 acgctcctcg atccgggcgg ccgctcgctg atgctctcgc atgtgaattc gcagaacatc
     3661 tgtttcacgt gaaacatcgt tgatggctca gacttcggtg cggtcgagct cgcggagcac
     3721 gcgggcgcgg tcaatgccga aggcggcgag catgcctttg actt
//
LOCUS       DAORPG010000121         3742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2077_length_3742_cov_3.679143, whole genome shotgun sequence.
ACCESSION   DAORPG010000121 DAORPG010000000
VERSION     DAORPG010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2077_length_3742_cov_3.679143"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PLX85_03775"
     CDS             <1..366
                     /locus_tag="PLX85_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119402.1"
                     /note="protein from Staphylococcus aureus has
                     phosphodiesterase activity against 2'-3'-cAMP and
                     2'-3'-cGMP; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRC62326.1"
                     /translation="VHCIAAHHEEVKPETTEALIVMIADAMSGSRPGARRESADQYVK
                     RLEALETIANSFDGVDQSFAIQAGREVRVIVRPDEIDDLAAARLARDVSKKIEETLQY
                     PGEIRVTVVRETRSSAVAR"
     gene            369..1145
                     /locus_tag="PLX85_03780"
     CDS             369..1145
                     /locus_tag="PLX85_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRC62327.1"
                     /translation="MRVLMVGDVVGRPGREAVSQLLPQLRASLDIDRVILNGENAAAG
                     RGLTEKTARELLSAGVDVITSGNHIFDVREFVPALDRDWPVLRPANYPLSTPGRGVCS
                     IGAFTVVSLMGRTFMPVQVDDPFRSIDALLETVSDDAAVFVDFHAEATSEKQAIAWYL
                     DGRVAAVVGTHTHVPTADARVLPGGTAMVTDLGMVGVRDSVIGDDVSAVLDRFLTSMP
                     TRLPVAEGAEVTFNSVLIDFDERTKRARSIERVDRECLLR"
     gene            1165..2025
                     /locus_tag="PLX85_03785"
     CDS             1165..2025
                     /locus_tag="PLX85_03785"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRC62328.1"
                     /translation="MSIGDFHTHSTRSDGRLSPTELVDLAAARGVRVLALSDHDTLDG
                     YEEAREAASKHPGFLLLPAVELSCDVPGTEVHMLGLCIDPLDTALLANLEQFRRGRIE
                     RAEKMVEALTRLGAPIEWSRVQEIAGEASVGRPHVAHALLERGHVQTFDEAFQRFLGR
                     NGPAYFEREKLAPADAIAMIRAAGGVAVFAHPSFTNDYEQVAADLAAQGLFGLETYYR
                     AYPPELVETLRALAVRLGLFPTGGSDFHGIDRNDERQPGEIPLPDEVVESFLERVRGL
                     GCNVPEPTPA"
     gene            2022..2543
                     /locus_tag="PLX85_03790"
     CDS             2022..2543
                     /locus_tag="PLX85_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62329.1"
                     /translation="MTTQDEVTTYCATHPDVESELRCGRCEKLICPRCLVYTPAGARC
                     RDCAQLRRPVMYELSTAHYAKAAGAALGVGAVVGVVGGLILPPDARVPLFGLFLALFA
                     GSGIGSVMAEAITRATRGKRGTAVQLIALGGILAAAVVRLLVSGQFNLAAQDVLGLFA
                     FGIAGVVAWGRLR"
     gene            complement(2553..2813)
                     /locus_tag="PLX85_03795"
     CDS             complement(2553..2813)
                     /locus_tag="PLX85_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62330.1"
                     /translation="MPRRPTTRGPLGRRSLPPSPTYGGPASSATAAASTPSGERRQRE
                     SRPARIVEREAPYLLAELRRIGMVTATCVGMLVMLVIVNHFK"
     gene            2916..2992
                     /locus_tag="PLX85_03800"
     tRNA            2916..2992
                     /locus_tag="PLX85_03800"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2950..2952,aa:Asp,seq:gtc)
     gene            3232..>3742
                     /locus_tag="PLX85_03805"
     CDS             3232..>3742
                     /locus_tag="PLX85_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRC62331.1"
                     /translation="MALIFVVLTACGGTDDEVPASGPGRLEVLCTVEESWCRVMVAAF
                     EAETGIATSYERLGSGDALAALRASKGSPKYSVWWGGPADGYVAAKAEGLLERYVAAN
                     ATSVPAASKDAEGAWTGVYVGALSFCSNRTELARLGLAAPGSWQELLSPALKGRISIA
                     HPSSSGTAYT"
BASE COUNT          559 a         1195 c         1376 g          612 t
ORIGIN      
        1 gtgcattgca tcgcggccca tcatgaggaa gtgaagcccg agacgactga agcgctgatc
       61 gtgatgatcg cagacgcgat gtcgggcagc cggccgggcg cccgccgcga atccgcggac
      121 cagtacgtga agcgcctcga ggcgttggag acgatcgcca attcgttcga cggtgtggac
      181 cagtcgttcg cgatccaggc cggccgcgaa gtgcgtgtca tcgtgcggcc cgacgagatc
      241 gacgacctcg cggcggcgcg actggcacgc gacgtttcaa agaagatcga ggagacgctg
      301 cagtacccgg gtgagatccg cgtcaccgtc gtgcgtgaga cgcggtcttc ggcggtcgcc
      361 agatagcgat gcgcgtactg atggtcggcg acgtggtcgg ccggcccggg cgcgaggccg
      421 tctcacaact gctgcctcag ctgcgggcat cgctcgacat cgaccgggtc atcctcaacg
      481 gcgagaatgc agccgccggg cgcggcctga cggaaaagac cgcccgcgaa ctgctgtcgg
      541 cgggtgtgga cgtcatcacc tcggggaatc acattttcga cgtgcgcgag ttcgtgcccg
      601 cgctcgaccg ggactggccg gtgctgcggc cggcgaatta cccgctctcg acccccgggc
      661 ggggtgtctg cagcatcggc gcgttcaccg tcgtgagcct gatggggcgt accttcatgc
      721 cggtgcaggt tgacgacccg ttccggtcga tcgacgccct gctcgagacg gtgtccgacg
      781 acgcggctgt gttcgtggac ttccatgcgg aagcgacgag cgagaagcag gccatcgcct
      841 ggtacctcga tggccgggtg gcagccgtgg tggggacgca cacgcacgtg ccgaccgccg
      901 acgcccgcgt gctgcctggc gggaccgcga tggtcaccga cctcggcatg gtaggcgtgc
      961 gcgactcggt gatcggggac gacgtgtcgg cggtgctgga ccgcttcctc acctcgatgc
     1021 cgacgcggct gccggtggcg gaaggcgccg aggtgacgtt taactccgtg ctgatcgact
     1081 tcgacgagcg gacgaagcga gcgcggagca tcgagcgggt ggaccgtgag tgcctgttgc
     1141 gatgagcgcg gcgacactgt cggcatgagc atcggcgact tccataccca ctcgacccgc
     1201 tcggacggtc ggctgtcccc caccgagctg gtagacctcg cggcggcgcg cggcgtgcgc
     1261 gtgctggcgc tgtccgacca cgacacgctc gatggctacg aggaggcccg cgaggccgcc
     1321 tcgaagcacc cgggcttcct gctgctgccc gcggtcgagc tgtcgtgcga tgtgccgggc
     1381 acggaggtgc acatgctcgg gttgtgcatc gacccgctgg acacggcgct gctggcgaac
     1441 ctcgagcagt tccggcgcgg ccggatcgaa cgggccgaga agatggtcga ggcgctcacg
     1501 cggctcggcg cgcccatcga atggtcgcgg gtacaggaga tcgccgggga ggcctcggtg
     1561 gggcggccgc acgtggcgca cgcgctcctc gagcgggggc atgtgcagac gttcgacgaa
     1621 gcattccagc gcttcctcgg gcggaacgga ccggcgtact tcgaacgcga gaagctggcg
     1681 cccgccgacg cgatcgcaat gatccgcgcg gccggaggcg tggcggtgtt cgcgcaccct
     1741 tcgttcacga acgactacga gcaggtggcc gccgacctgg ccgcacaggg gctgtttggc
     1801 ctcgagacgt attaccgagc ctatcccccg gaactcgtgg agacgctgcg cgcgctcgcc
     1861 gtccggctcg ggctgttccc cacgggtggg tccgacttcc acgggatcga ccgcaacgac
     1921 gagcggcagc cgggcgagat cccgctgccg gatgaggtgg tcgagtcatt cctcgaacgg
     1981 gtgcgaggcc tcggctgcaa tgtcccggag ccaacaccgg cgtgacgacg caagacgagg
     2041 tgacgaccta ctgcgcgacg catccggacg tcgagagcga gctgcgctgc gggcgctgcg
     2101 agaagctgat ctgccctcgc tgcctggtgt acacgcccgc cggcgcgcgg tgccgggact
     2161 gcgcgcagct tcgccgtccg gtgatgtacg agctgtcgac ggcgcactac gccaaggcgg
     2221 cgggcgctgc cctcggagta ggcgcggtcg tcggcgtggt cgggggtctc atcctgccgc
     2281 cggacgcgcg cgtcccgctg ttcgggctgt ttctcgctct gttcgcgggc tccggaatcg
     2341 gtagcgtcat ggcagaggcg atcacgcgag cgacgcgcgg caagcggggg acggcagtcc
     2401 agctcatcgc gctcggtggc atcctcgcgg ccgctgtcgt gcggctgctg gtcagcgggc
     2461 agttcaacct cgccgcacag gatgtgctag ggctgttcgc gttcggaatc gccggtgtcg
     2521 tggcgtgggg gcgtctgcgg taggctgcgc ggctacttga agtggttcac gatcacgagc
     2581 atgacgagca tgccgacgca ggtggctgtg accatgccga tgcgccgcag ctccgcgagc
     2641 aggtagggag cctcgcgctc aacgatgcgc gccggacggc tttcgcgctg cctgcgctcg
     2701 ccggaaggtg tggaggcagc cgcagttgcg ctgctcgctg gccctccata ggtcgggctg
     2761 ggcgggagcg agcggcggcc cagggggcct cgggtcgtgg gacgcctcgg catgtcttcc
     2821 tccgagggat agtgtagcga gggcccggct caggcgaaac gcgggctcgt ttgacccctc
     2881 gcgcgggtgt cgatacgatc cagttcgctc cacgcggccc cgtagtgtag cggcctaaca
     2941 cgccaccctg tcacggtgga gatcgtcggt tcgaatccga tcggggtcgc cattccttcg
     3001 cctgcctccc gagctgcggt gggatcgacc ggcagtcggc ctcgcgagga ctcccaattt
     3061 cgcgccaagc ttcatgatgc gctggctgtt gcgccatacg cggtgaccgc gttcatatgc
     3121 aataacgccc cgcggattca gttgcgttga agctaggact acaccatgcg gttgcgtgat
     3181 ggcctgtcct tcgtcgcacg agggcgcaga gcggcgttgc cagcagcaac cgtggccttg
     3241 atcttcgtcg tcctgaccgc ctgcggcggc accgacgacg aagtgccggc gagtggaccg
     3301 ggtcgcctcg aggtgttgtg taccgtcgag gagagctggt gcagggtgat ggtggcggcc
     3361 ttcgaagccg aaaccgggat cgccacaagc tacgaacggc tgggttccgg ggacgcgctg
     3421 gcggcgctcc gggcctcgaa gggtagcccg aagtacagcg tgtggtgggg cggcccggcg
     3481 gacggatatg tcgcggcgaa ggcggagggc ctcctcgagc ggtatgtcgc ggcgaacgcg
     3541 acatctgtgc cggccgcgtc gaaggacgcc gagggcgcgt ggacgggcgt gtatgtcggg
     3601 gcactgagct tctgctcgaa ccggacagag ctcgccaggc tcggactcgc agcgcccggg
     3661 tcttggcagg agttgctcag ccccgcactc aagggacgca tctcgatcgc acatccttcg
     3721 tcctcaggga ccgcgtatac gg
//
LOCUS       DAORPG010000122         3740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2080_length_3740_cov_3.708277, whole genome shotgun sequence.
ACCESSION   DAORPG010000122 DAORPG010000000
VERSION     DAORPG010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2080_length_3740_cov_3.708277"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PLX85_03810"
     CDS             complement(<1..473)
                     /locus_tag="PLX85_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC62332.1"
                     /translation="MIAVDIQKRLGDFALDVAFEAGHETVVLFGHSGSGKSVSLAAIA
                     GLLRPDSGRIEVAGRVVFDATRGVNLPPQRRNVGYVVQQLALFPHLTAAENIGYGLTR
                     TSATARRARIAELVGLLSLAGLEDRLPRQLSGGQQQRVALARALARPVDVLLLDEP"
     gene            complement(477..1319)
                     /locus_tag="PLX85_03815"
     CDS             complement(477..1319)
                     /locus_tag="PLX85_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC62333.1"
                     /translation="MTSLADRPVASEGRTSPSLATLFALPALLFGAFVLVPLAALVYR
                     AVRDEGVAGRLTDASVQTALRLSLVTSTLTLAIALLLGIPLAYLLARTQFPGKQLVDT
                     LVDLPMVLPPTVAGVALLVAFGRRGVFGSVLDRLGIELAFTTSAVVLAQLFVSAPFLV
                     RSLKAGFQAVDPTYEQVSATLGVSPLRTFWRITLRLSRPTLVGGAVLCWVRALSELGA
                     TIIFAGNFEGRTQTMPLAIISAFESSQGLAGAMALAVILLAVAFVLLVVLRLVLRGDR
                     LFEV"
     gene            complement(1407..2243)
                     /gene="modA"
                     /locus_tag="PLX85_03820"
     CDS             complement(1407..2243)
                     /gene="modA"
                     /locus_tag="PLX85_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRC62334.1"
                     /translation="MLLTMVVASLVSVGVACGGSDRGATSANGIPATSSAGATRLQGK
                     VTVFAASSLTESFKAIGEAFTNANPGVTVEFNFASSSALATQIEQAAPADVFASADQP
                     QMQRLVDDRLVDGTAEVFARNLPVVVVPAANRAGLTTPKDLAKSGIKLVLAAQDVPIG
                     NYARQIIDRLAADPAYGAAFKDAALRNLVSNEANVRAVLTKIELGEGDAGIVYTTDAL
                     VSKEKVRTIAIPEGVNVIASYPIAVLRTSKNAVAAAAFVAFVRGPEGQSLLKAGGFNA
                     AP"
     gene            complement(2312..2905)
                     /locus_tag="PLX85_03825"
     CDS             complement(2312..2905)
                     /locus_tag="PLX85_03825"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRC62335.1"
                     /translation="MHNADRVRVGIVTARRAPAPLVRDEPLSAVQYAILGLLRGQAAH
                     GYELQRSFTAGSDLAGVVHLEQPSLYAALKDLAGRGLIEGTEAREGLRPPRTVYSLSK
                     RGERLLDVWMRTPVERLRQVRLDFLLKVYFARQRGEAAVRALVDAQIATCHRYLADLE
                     ARAAELGPESFAYLVTESRTSAARSTLEWLREYRHRV"
     gene            3059..>3740
                     /locus_tag="PLX85_03830"
     CDS             3059..>3740
                     /locus_tag="PLX85_03830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC62336.1"
                     /translation="MQYVDAATRLRPAATEALDAYTSLVAANREQVERLRDTPEPEGD
                     FWARRAPRFRPGVLDVEELPGLLELAQPEDVWLDVGAGGGRFAVPLSRHVKRVIAIEP
                     SPAMRGMLADSIAAAERDNLEVVDLRWPPEPGIESPSGDVALVANVLYDCLVLGEFIA
                     ALEAHVSRRCVVIASDRAPSTPDPAVWEALYGEPLCALPGLPEFVAVLGAMKRRYEVR
                     AFGMSSPPP"
BASE COUNT          637 a         1251 c         1310 g          542 t
ORIGIN      
        1 ggctcgtcga ggagcaggac atccacgggt cgggccagcg cgcgagccag cgcgacgcgc
       61 tgctgctgac cgccggacaa ctgccgcggc agtcgatcct cgagacctgc caggctgagg
      121 aggccgacca gttccgcaat ccgggcgcgg cgggcggtcg ctgaggttcg ggtaaggccg
      181 taaccgatgt tctcggccgc agtgaggtgg ggaaacagag ccagctgctg cacgacgtag
      241 ccgacgttgc gccgctgcgg cggcaggttg acgccgcgcg tggcgtcgaa aaccacgcgg
      301 ccggcgacct cgatacgtcc ggagtccggg cgcagcagcc cggcgatcgc cgccagcgag
      361 acgctcttgc ccgaacccga gtggccgaag agcaccaccg tctcgtgccc tgcctcgaag
      421 gcgacgtcga gagcaaagtc accgaggcgc ttctggatat cgaccgcgat cacccgtcac
      481 acctcgaaga ggcgatcgcc ccgcaagacg aggcgcagca cgacgaggag cacgaaggcg
      541 accgcgagca agatgacggc gagcgccatc gcgcccgcga gtccctggct agactcaaat
      601 gcgctgatga tcgcgagcgg catcgtctgc gtccggccct cgaagttgcc ggcgaaaatg
      661 atcgtcgcgc cgagctcgct cagggcacgt acccagcaga ggaccgcgcc gcccacgagc
      721 gtgggacgcg agagccgcaa cgtgatacgc cagaacgtcc gcagtggcga cacacccagc
      781 gtggcggaca cttgctcgta cgtcgggtcg accgcctgga agcccgcctt caggctacgc
      841 acgaggaagg gcgcggagac gaacagctgc gccaggacga cggccgaggt tgtgaaggcg
      901 agctcgatgc cgaggcgatc gagcactgac ccgaacacgc cgcgccgccc gaaagcgacg
      961 agcagcgcca cgcccgcgac cgtcggcggc agcaccatgg gcaggtcgac cagcgtgtcc
     1021 accagctgct tcccggggaa ctgcgtcctc gccaggaggt aggcgagggg aatgcctagc
     1081 aggagcgcga tagcgagcgt caaggtgctc gtcaccagcg agagccggag ggcggtttgc
     1141 accgatgcgt ccgtcagccg gccggcgacc ccttcatcgc gcaccgcgcg gtacacgagc
     1201 gcagccagcg ggacgaggac gaaggcgccg aagagcaacg ccggcagcgc aaagagagtg
     1261 gccagcgagg gactcgtgcg cccctcgctg gccaccggac ggtccgccag agaggtcata
     1321 cctcagagtg ctcttcgccc gacgctcggc gcaaggggcg cctgacgggc atcggagccg
     1381 cggagcagac gaggattgcg gcgatgtcac ggggcggcat tgaagccccc tgccttcagt
     1441 aaggactgac cttcaggccc gcgcacgaac gcgacgaacg cagccgcggc cacggcgttc
     1501 ttcgatgtcc tgagcacggc gatcggatac gacgcgatca cattcacgcc ttcagggata
     1561 gcgatggtcc tgaccttctc tttcgagacc agcgcgtcgg ttgtgtagac gatgccggca
     1621 tcgccctcgc cgagttcgat cttggtgagc acggcgcgca cgttcgcctc gttcgagacg
     1681 aggttcctga gcgccgcgtc cttgaatgcc gcgccgtagg cagggtcagc cgcgagcctg
     1741 tcaatgatct ggcgcgcgta attgccgatc ggcacgtcct gggcggcgag cacgagcttg
     1801 atgccagact tcgcaaggtc cttcggcgtc gtgaggccgg cgcggttagc ggcgggaacg
     1861 acgaccacgg gcaggttgcg cgcgaagacc tcggcagtgc cgtctacgag cctgtcgtcc
     1921 accaggcgct gcatctgcgg ctgatcggcg ctggcgaaca cgtcggccgg cgccgcctgc
     1981 tcgatctgtg tggccagcgc gctcgaggag gcgaaattga actcgaccgt tacacccggg
     2041 ttggcgttcg tgaaggcctc gccaatcgct ttgaacgact cggtcaacga ggaagccgcg
     2101 aaaacggtga ctttgccctg gagccgggtg gcgcctgccg agctggtcgc cggtatcccg
     2161 ttagccgacg tggcgccgcg gtcggaacca ccgcaggcga cgccgacact gaccagggag
     2221 gcgaccacca tggtcaggag caccgctggc caccaccttc ggctgccgtt catacgcatc
     2281 ccattccgct ctgactgcga ttccgctggc cctacacgcg gtggcgatac tcacgaagcc
     2341 attcgagcgt gctgcgcgcg gcgctcgtcc gggattccgt cacgaggtag gcgaagctct
     2401 cggggccgag ctcggccgcc cgcgcctcga ggtcggcgag gtagcgatgg caggtggcga
     2461 tctgcgcgtc gacgagggcg cgtacggcgg cttcgccgcg ctgacgggcg aaatacacct
     2521 tgagcaagaa gtcgaggcgc acctggcgga gtcgctcgac cggagtccgc atccagacat
     2581 cgagcagacg ctcgccgcgc ttcgacagtg agtacaccgt gcgcggcggg cgcaggcctt
     2641 cgcgtgcctc ggtgccttcg atcagccctc ggccggcgag atccttcagc gcggcataca
     2701 aactcggctg ttcgaggtgc acgacgccgg cgaggtcgct gcccgcggtg aacgagcgct
     2761 gcaattcgta gccatgcgcg gcttggcctc gcagcagacc gaggatggcg tactggacgg
     2821 ccgagagcgg ctcgtcgcgg acgagcgggg ccggcgcacg gcgggcggtc actattccca
     2881 cccgcacgcg gtcggcgttg tgcacgggct actcattgat cgcatctgtt gtcccgatta
     2941 ctcaaagcct gagtatcatg ggcgagagta tcgttgcccg ccatcccggt gcaatggcta
     3001 ccatgtcctg ccctcgtgcc agggtcgtca cgccggtgcg cgaatgaagg agcgaacgat
     3061 gcagtacgtg gatgccgcca cgcggctgcg gcccgcggcc accgaggccc tcgacgccta
     3121 cacctccctc gtggcggcca accgcgagca ggtcgaacgc ctgcgcgata cgccggaacc
     3181 ggaaggcgat ttctgggcac gccgggctcc gcggttccgt ccgggcgtcc tcgatgtcga
     3241 ggagcttccg gggttgctcg agctcgctca gccagaggac gtgtggctcg acgtcggggc
     3301 ggggggcggc cgctttgccg tcccgttgag ccggcatgtg aaacgagtaa tcgccatcga
     3361 gccgtcgccg gcgatgcgcg gcatgctcgc cgatagcatc gcggccgccg agcgcgacaa
     3421 cctcgaggtc gtcgacctgc ggtggccacc cgaaccgggt atcgagtcgc cgagcggcga
     3481 cgtggccctc gtggcgaacg tgctctacga ctgcctggtg ctcggggagt tcatcgcggc
     3541 cctcgaagcc cacgtatcga ggcggtgcgt ggtgatcgcg tccgaccgcg ccccgagcac
     3601 gcccgacccg gcggtgtggg aagcgctcta cggcgaaccg ctctgcgcgc tgccggggct
     3661 ccccgagttc gtggcagtcc tgggcgcgat gaaacgccgc tacgaggtgc gagcctttgg
     3721 gatgtcgagc ccgcctccac
//
LOCUS       DAORPG010000123         3718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2098_length_3718_cov_4.076440, whole genome shotgun sequence.
ACCESSION   DAORPG010000123 DAORPG010000000
VERSION     DAORPG010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2098_length_3718_cov_4.076440"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PLX85_03835"
     CDS             <1..195
                     /locus_tag="PLX85_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HRC62337.1"
                     /translation="TDVVKALALGADAVLLGRPVLWGLAVEGEAGVDHVLRLLRSEFE
                     HAMLLCGAATRGDLTADLLA"
     gene            269..1399
                     /locus_tag="PLX85_03840"
     CDS             269..1399
                     /locus_tag="PLX85_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRC62338.1"
                     /translation="MQLALLIAVVLVGLLAAFTLLIAYNLVMQNGRILLRLERLESVF
                     VAPPDPAALVEDYIPNLPPGTPAPPFELPDLNGIPRRLTEWRGQRVLLVFFDPECAFS
                     RRLLPLLVGLTRDVVPGRPAPVIVSTGAIEANRRLFDASDFSGAVLLQRATEVAAAYR
                     VDGTPMSYLLDADGTTVGEVAVGVQAIMLQAGEIGSVEDAVLDDGGASPALLDAPVTR
                     RDGLTPGEAAPLFRLPSAAGGELSLLDFRGRATVIAFIDPECEACDQVTSLLESVHRE
                     HPQLGVLAISRGDPQLTRIDIARHDATFAVGLQRHWEVSRRYGIFATPAAFLLDEWGV
                     VSAEVAVGAASINQLLVSAGRAADQMEIARAQPSGDLERDDL"
     gene            1417..1716
                     /locus_tag="PLX85_03845"
     CDS             1417..1716
                     /locus_tag="PLX85_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62339.1"
                     /translation="MSKHIAGKHSRRGLFRLLGAGFLGAALAGTFARGADAGDPKAET
                     LPFFNGTLPYFNFNLPQFNHTYPKPKPPVWNGYIPPVFNGTYPPIFNGTFPPHFN"
     gene            1801..2589
                     /locus_tag="PLX85_03850"
     CDS             1801..2589
                     /locus_tag="PLX85_03850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRC62340.1"
                     /translation="MVSVVLTTRDRPHLLPIALRCFQHQSYANRELVVVDDGLDFPAD
                     VSSLPAESVRVVTVPPGTPLGVKLNHGVEAARGRLCMKMDDDDWYAPDYLATSVEALL
                     DSERLVCRPTIVFHMGFLFFELKSWRIHESTDFNAPGATLLFSKNDWRRRPFRPVPTD
                     EDAWFYRDQIRAGAQFLTIDSKETFVAIRHRGHSGTFGHTWTYQLDGSTLEQYLTSRP
                     LYHRSPEQLFPDWALDVYRGIHDELVAAEAAVSRAETDAAPVRP"
     gene            2567..3361
                     /locus_tag="PLX85_03855"
     CDS             2567..3361
                     /locus_tag="PLX85_03855"
                     /inference="COORDINATES: protein motif:HMM:NF015417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62341.1"
                     /translation="MRLPFARRQGSEASAGDRLHPRVLILTPMKDARPTLDNYVRLLR
                     RLTYPHDRISLGFLEGDSADGTFEALRVVQPQLDREFRRALIWKRDFGYRIPDGLPRW
                     DESIQFERRSILARSRNHLLFHALDDEDWVLWMDADLLDYPADVIERLLAVGKDIVHP
                     HCVLQFGGPTFDQNAWVDQGQDHMDVLRDHELVRLDAVGGTMLLVNADLHRDGLVFPP
                     FMYGLESPRVRRGRGEIETEGLAALAHDMGVECWGLPNLEILHHPF"
     gene            3456..>3718
                     /locus_tag="PLX85_03860"
     CDS             3456..>3718
                     /locus_tag="PLX85_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62342.1"
                     /translation="MRAGMWLEALAQRYRVALLVVPVFAAASPRFEAFARAHAESVTV
                     MAPVPASYRRWPRLFRRRDPDLIRVLGAQARRCVDAVRSLDGSA"
BASE COUNT          536 a         1381 c         1142 g          659 t
ORIGIN      
        1 accgacgtcg tgaaggcgct cgccctcggc gccgacgccg tcctcctcgg acgcccggtc
       61 ctctggggac tggccgtcga gggtgaggcg ggcgtcgacc acgtcctgcg cctcctgcgt
      121 tcggaattcg agcacgcgat gttgctctgt ggcgccgcaa cccggggcga cctgacggcc
      181 gatctgctgg cctgaccgct ataccttccg cgcgtttcgt ccttctgcca cactccgcac
      241 gaagcgcgcg cggtgtttgg agcatgtcgt gcagctcgcc ctgctgatcg ccgtcgtgct
      301 ggtcggcctg ctcgcggcgt tcacgctgct gatcgcctac aacctcgtca tgcagaacgg
      361 gcgcatcctg ctgcgcctcg aacggctcga gtcggtcttc gtggcgccgc ccgatcccgc
      421 ggcgctggtc gaggactaca tccccaacct gccaccggga acaccggccc cgcccttcga
      481 gttgcccgac ctcaatggca ttccccgtcg tctcactgag tggcgtggtc agcgggtgct
      541 gctcgtcttc tttgacccgg agtgcgcctt ctcgaggcgt ctgctgccgc tgctcgtcgg
      601 cctgactcgc gatgtcgtcc ccggccgccc cgcgccggtg atcgtctcga ccggggcgat
      661 cgaggcaaac cggcggctct tcgacgcgtc ggacttcagc ggagcggtgc tcctccagcg
      721 cgccaccgag gtggccgccg cctaccgggt cgatggcact cccatgagct acctgctcga
      781 tgcggatggc acgacggtcg gggaggtcgc cgtcggcgtc caggccatca tgctgcaggc
      841 gggggagatc ggctccgtcg aggatgcagt cctcgatgac gggggcgcga gccccgcgct
      901 cctcgacgcc cctgtgacgc gccgcgacgg cctcactccc ggcgaagcgg ctccgctctt
      961 tcggcttccg tcggccgcgg gaggcgagct ctcattgctc gactttcgag gccgcgcaac
     1021 agtcatcgcg ttcatcgatc cggaatgcga ggcctgcgat caggtcacgt ccctcctcga
     1081 gtcggtacac cgcgaacatc cgcaactggg cgtcctcgcg atcagccgag gcgatcccca
     1141 gctcacccga atcgacatcg caaggcacga cgccaccttc gcggtcggcc tgcagcggca
     1201 ctgggaagta tcaaggcgtt atggtatctt cgcgacgccc gcagcgttcc tgctcgacga
     1261 gtggggcgtg gtgagcgccg aggtggccgt gggagcggcg agcattaacc agctcctcgt
     1321 gtctgccgga cgcgcggccg atcagatgga gatcgcgagg gctcagccct cgggggacct
     1381 cgaaagggat gacctgtgac cgagcgattc gatgacatga gcaagcacat cgcggggaag
     1441 cactcgcgcc gagggctgtt ccgcctgctg ggcgcgggct tcctcggggc cgcccttgcc
     1501 gggacctttg cccgtggggc cgacgccggc gaccccaagg ctgagacact gcccttcttc
     1561 aacggcacgc tgccctactt caacttcaac ctgccgcagt tcaatcacac ctacccgaag
     1621 ccgaagccgc cggtctggaa cggctacatc ccacccgtct tcaacgggac gtatccgccc
     1681 atcttcaacg gtaccttccc accgcacttc aactagtccg gacacccgtc cgggctccgt
     1741 tccgtgccag tccccgggcg gcccgaggcg gtgccatcca cggcgccggc ctcgggcccc
     1801 ttggtttctg tcgtcctgac gacgcgcgac cggccgcacc tcctgccgat tgcgctccgc
     1861 tgctttcaac accagagcta cgccaaccgc gagctcgtcg tcgtcgacga cgggctggac
     1921 ttcccggccg acgtctcatc gcttcccgcc gagtcagtcc gcgtcgttac cgtcccccct
     1981 gggacaccgc tcggcgtgaa gctcaaccac ggcgtcgagg cggctcgggg tcgtctctgc
     2041 atgaagatgg acgatgacga ctggtacgca cctgactacc tcgccacgtc cgtcgaggcc
     2101 ctgctcgaca gtgagcgcct ggtctgtcgt ccaaccatcg tctttcacat gggcttcctc
     2161 ttcttcgagc tcaagtcctg gcgcattcat gaatccaccg acttcaacgc cccgggcgcc
     2221 acgctgctgt tttcgaagaa cgactggcgt cggcggccct tccgccccgt ccccacggat
     2281 gaggacgcct ggttctaccg tgaccagatc cgcgcgggcg cccagttcct caccatcgac
     2341 tcgaaggaga ccttcgtcgc catccgccat cgaggccatt ccggaacgtt cggccacacc
     2401 tggacctacc agctcgacgg cagcacgctc gagcagtacc tgacctctcg tccgctctac
     2461 caccgctccc ccgaacagct cttccccgac tgggcgctgg acgtgtatcg aggtatccac
     2521 gacgagctcg tcgcggccga agcggccgta tcgcgtgctg agaccgatgc ggctcccgtt
     2581 cgcccgtagg caaggttccg aggcgtccgc gggcgaccgc ctacaccccc gcgtcttgat
     2641 cctgacgccg atgaaggacg cacgtcccac actggacaac tacgtgcggc tcctccgacg
     2701 actcacttac ccccacgacc gcatctcgct cggcttcctc gagggcgaca gcgccgacgg
     2761 cacattcgag gcgctacgcg tcgtccagcc tcagctcgac cgggagttcc gccgcgccct
     2821 gatctggaaa cgggacttcg ggtaccgcat tcccgacggc cttccgaggt gggacgagtc
     2881 gattcagttc gagcgccgca gcatcctcgc gcgcagccgc aaccatctcc tgttccatgc
     2941 gctggacgac gaggactggg tgctctggat ggacgccgac ttgctcgact accccgcgga
     3001 cgtgatcgag cgtttgctcg cggtcgggaa ggacatcgtt cacccgcact gcgtcctgca
     3061 gttcggagga cccaccttcg accagaacgc ctgggtcgac cagggtcagg accacatgga
     3121 cgtccttcgc gaccacgaac tcgtgcgcct cgatgcggtc ggcggcacga tgctcctcgt
     3181 gaatgccgac ctgcaccgcg acggcctcgt gttcccgcca ttcatgtacg gtctcgaaag
     3241 tccgcgtgtg cgccgcggcc gaggcgagat cgagactgag ggcctggccg cgctcgcgca
     3301 tgacatgggc gtcgagtgct gggggctccc caacctggag atcctccatc acccgttctg
     3361 actcgcggcc gccgaccctc gacgaccgag gcgctcggcc gcgcctcctg tttgtcacgc
     3421 cgacattccc ggacaccggc ggcaacggcc tcgcgatgcg cgccggcatg tggctcgagg
     3481 ccctcgctca gcggtaccgg gtcgcgctcc tcgtcgtgcc ggtattcgcc gccgcgtccc
     3541 cccggtttga ggcattcgcg cgcgcgcatg ccgagtcagt gacggtcatg gcgcccgtgc
     3601 ccgcgtccta ccgccgctgg ccgcgcctct tccgccgacg cgaccccgat ctcatccgcg
     3661 ttcttggcgc gcaggcacgc cgctgcgtcg acgccgtccg ctcgctcgat ggctcggc
//
LOCUS       DAORPG010000124         3691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2125_length_3691_cov_3.916942, whole genome shotgun sequence.
ACCESSION   DAORPG010000124 DAORPG010000000
VERSION     DAORPG010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2125_length_3691_cov_3.916942"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..798)
                     /gene="leuD"
                     /locus_tag="PLX85_03865"
     CDS             complement(196..798)
                     /gene="leuD"
                     /locus_tag="PLX85_03865"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043713907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRC62343.1"
                     /translation="MQPVKDITGNAIPLNRADVDTDQIMPKQFLKRIERSGFGPFTFF
                     DWRKDPNFVLNNAAYEGAPILVAGPNFGCGSSREHAPWGLEDMGIQAIVAPSFADIFR
                     NNCAKIGLLTVVLPQEDVDYLIARAEELPAQRIKVSLAQQTVSIEGTDWARHFDVDPF
                     VKHCLLEGLDDIGLTLQHDEDIAKFESTRSPLLPTTSTAA"
     gene            complement(798..2204)
                     /gene="leuC"
                     /locus_tag="PLX85_03870"
     CDS             complement(798..2204)
                     /gene="leuC"
                     /locus_tag="PLX85_03870"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRC62344.1"
                     /translation="MTTPKTLSEKIWEQHLVRQEEGQPDLLYVDLHLVHEVTSPQAFE
                     SLRMSGRKVRRPDLTMSTVDHNIPTIGRDLPNPDPLSQQQIETLRTNCKEFGIQLFDM
                     FDLRQGIVHIIGPEQGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTL
                     LQARPKTMSIEVNGELGFGVTAKDVILAIIRKIGVDGGVGHVIEYRGDVIRNLSMEGR
                     MTICNMSIEGGARAGLVAPDETTFAYMQGRPYVPKGEDWDRAVEAWRKLPTDEGAKFD
                     TYVTLDGAAIEPHVTWGTTPAQSVPVSGRVPSPEDAATASARETAERSLTYMDLKAGT
                     RIQDIAIDRVFLGSCTNGRIEDLRAAAKVVSGKHVASTVRAMVVPGSGLVKAQAEREG
                     LDQIFKDAGFEWRDAGCSMCLGMNPDILMPGERCASTSNRNFEGRQGAGGRTHLVSPE
                     MAAAAAVAGHFVDIREVL"
     gene            complement(2225..3013)
                     /locus_tag="PLX85_03875"
     CDS             complement(2225..3013)
                     /locus_tag="PLX85_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRC62345.1"
                     /translation="MDYSRYPTLEVERNGPVLTVRMNRPESLNAINAQLHTDLSFVFA
                     DIAQDEETKAVILTGAGRAFSAGGDIQWFTRMTPDELDTLFIEARKLIVDMLEVPQPI
                     ISVLNGATIGLGATIALMCDVIFMDERARIGDPHVTMGLVAGDGSAIIWPWLIGMARA
                     KQYLMTGDLIEAAEAERIGLVNFVRPADELMPDAMAFAERLANGPTRAIRATKHSVNK
                     LLRDSVNIVLDTSLALEKESFTSEDHKEGVAAFIEKRQPNFRGR"
     gene            complement(3066..>3691)
                     /locus_tag="PLX85_03880"
     CDS             complement(3066..>3691)
                     /locus_tag="PLX85_03880"
                     /inference="COORDINATES: protein motif:HMM:NF020091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HRC62346.1"
                     /translation="TFEIMAPADIGVPVGDSIVLTKVSGRHGLRARIAALGVNLEADE
                     FERVYEAFKDLADRRDEIDDRDLEAILAEQTSVQISEQWTLDLVQVSAGDHAAPTATV
                     RLIGPDGAAHQDAAIGTGPVDAVYQAINRVMKVSNELTEFSVKSVTEGIDAQGEVSIR
                     IEAEGETYSGRAADTDILVASARAYMHALNRYLNVAARRKAELTATR"
BASE COUNT          519 a         1212 c         1309 g          651 t
ORIGIN      
        1 cacgtcccgg acttcggcac gcggacggcg agattgccga cgcggtaggc ggtcgtacac
       61 cagccctcgc cgaggcgttc agccgcggcg agatcgcggg cgcgcagaaa cggaaactcc
      121 gcccgcagac gcgagcggag ttccgaggtg agcggattcg tcgaggccgg cacgcggccg
      181 aacctcgaag cgctgctagg cggccgtcga ggtcgtcggc agcagcggcg agcgtgtcga
      241 ctcgaacttc gcgatgtcct cgtcgtgctg cagcgtcagg ccaatgtcgt cgagaccctc
      301 gaggaggcag tgcttcacga aggggtcaac gtcgaagtga cgcgcccagt cggtgccctc
      361 gatggagacg gtctgctgcg ccaggctgac cttgatgcgc tgcgcgggaa gctcctcggc
      421 gcgggcgatc aggtagtcga cgtcctcctg cgggagcacg acggtcagca ggccgatctt
      481 cgcgcagttg ttgcggaaga tgtccgcgaa ggagggagcg acgatcgcct ggatgcccat
      541 gtcctcgagg ccccagggag cgtgctcacg cgaggagccg cagccgaagt tgggccccgc
      601 gacgaggatg ggtgcgccct cgtaggcggc attgttgagg acgaagtttg gatccttgcg
      661 ccagtcgaag aaggtgaagg ggccgaaccc gctgcgctcg atgcgcttca ggaactgctt
      721 cggcatgatc tggtcggtgt ccacgtccgc gcggttgagc gggatcgcgt tgcccgtgat
      781 gtccttgacc ggctgcatta cagcacctcc cggatgtcga cgaagtggcc ggcgacggcg
      841 gcggcggccg ccatctccgg gctgacgagg tgggtgcggc cacccgcgcc ctggcgtccc
      901 tcgaagttgc ggttcgaggt ggaggcgcag cgctcgcccg gcatgaggat gtcggggttc
      961 atgcccaggc acatcgagca gcccgcgtcg cgccattcga agcccgcgtc cttgaagatc
     1021 tggtccaggc cctcgcgctc cgcctgcgcc ttcacgaggc cggagcccgg caccaccatt
     1081 gcgcgcaccg tgctcgcgac gtgcttgccg gagacgacct tcgccgccgc ccgcaggtcc
     1141 tcgatgcgcc cgttcgtgca ggagccgagg aacacgcggt cgatggcgat gtcctggatg
     1201 cgtgtgccgg ccttcaggtc catgtaggtg agcgagcgct ccgccgtctc ccgcgccgag
     1261 gcggtcgctg cgtcttccgg gctcggcacg cggccgctca ccgggacgct ctgcgcgggc
     1321 gtcgtgcccc aggtgacgtg cggctcgatg gccgccccgt cgagggtgac gtaggtgtcg
     1381 aacttcgcgc cctcgtcggt gggaagcttg cgccacgcct cgacggcgcg atcccagtcc
     1441 tcgcccttcg gcacgtaggg gcgaccctgc atgtaggcga aggtggtctc gtccggggcg
     1501 acgaggccag cgcgtgcccc gccctcgatc gacatgttgc agatcgtcat ccgtccctcc
     1561 atcgagaggt tgcggatgac gtcgccgcgg tactcgatga cgtggccgac gccgccgtcc
     1621 acgccgatct tccggatgat cgcgaggatg acgtccttcg cggtgacgcc gaagccgagc
     1681 tcgccgttca cctcgatcga catcgtcttg ggccgcgcct gcagcagcgt ctgcgtcgcc
     1741 agcacgtgct cgacctcgga agtgccgatg ccgaaggcga gcgcgccgaa cgcgccgtgc
     1801 gtcgaggtgt gcgagtcacc gcagacgatg gtcatgcccg gctgggtcag tccctgctcg
     1861 gggccgatga tgtgcacgat gccctggcgc aggtcgaaca tgtcgaagag ctggatgccg
     1921 aactctttgc agttcgtgcg cagcgtctcg atctgctgtt gcgagagcgg gtcggggttg
     1981 ggcaggtcgc gtccgatggt cgggatgttg tggtcgaccg tcgacatcgt gaggtcgggg
     2041 cgtcgcacct tgcggccgct catgcggagc gactcgaagg cctgcggcga cgtcacctcg
     2101 tggacgaggt ggaggtcgac gtagagcagg tcgggctgcc cctcctcctg gcggacgagg
     2161 tgctgctccc agatcttctc ggacagcgtc tttggagtgg tcatcgttcc tccctgttgt
     2221 ggcgtcagcg cccgcggaag ttcggctggc gcttctcgat gaacgccgcc acgccctctt
     2281 tgtggtcctc gctcgtgaag ctctcctttt cgagcgcgag cgaggtgtcg agcacgatgt
     2341 tcacgctgtc gcgcagcagc ttgttcacgg agtgcttggt cgcgcggatc gcgcgcgtgg
     2401 ggccgttggc gaggcgctcc gcgaacgcca tcgcgtcggg catcagctcg tcagccgggc
     2461 gcacgaagtt cacgaggccg atgcgctccg cctcggcagc ctcgatcagg tcgccggtca
     2521 tcaggtactg cttcgcccga gccatgccga tgagccaggg ccagatgatg gcgctgccgt
     2581 cgccggcgac gaggcccatc gtcacgtgcg gatcgccgat gcgcgcacgc tcgtccatga
     2641 agatgacgtc gcacatgagc gcgatcgtcg caccgaggcc gatcgtggcg ccgttcagca
     2701 cggagatgat cggctgcggc acctcgagca tgtcgacgat gagcttgcgc gcctcgatga
     2761 agagggtgtc gagctcatcg ggggtcatgc gcgtgaacca ctggatgtcg ccgcccgccg
     2821 agaaggcgcg gccggcgccc gtgaggatga cggccttcgt ctcttcgtcc tgcgcgatgt
     2881 ccgcgaagac gaaggagagg tcggtgtgca gctgggcgtt gatcgcgttc agcgactccg
     2941 gccggttcat ccgcaccgtg agcactggcc cgttgcgctc gacctcgagc gtcgggtacc
     3001 ggctgtagtc catcactccc cctcgtcctc gacaggtgcg cgactcattc tcgtggcgtc
     3061 cgaggttaac gcgtggcggt cagctccgcc ttgcggcgcg cggcgacgtt caggtagcgg
     3121 ttgagtgcat gcatgtaggc acgggcgctg gccacgagga tgtccgtgtc ggcggcgcgg
     3181 ccgctgtacg tctcgccctc ggcctcgatg cggatgctga cctcgccctg cgcgtcgatg
     3241 ccttcggtga ccgacttcac gctgaactcg gtcagctcgt tgctcacctt catgacgcgg
     3301 ttgatcgcct ggtagacggc gtcgacgggg ccggtgccga tggcggcatc ctggtgcgcc
     3361 gctccgtcgg gcccgatcag ccgcaccgtt gccgtcggcg cggcgtggtc gcccgccgac
     3421 acctgcacca ggtcgagcgt ccactgctcc gagatctgca ccgaggtctg ctcggccagg
     3481 atggcctcga ggtcgcggtc gtcgatctcg tcgcggcggt cggccaggtc cttgaacgcc
     3541 tcgtagaccc gctcgaactc gtccgcctcg aggttgacgc ccagcgccgc gatgcgcgcg
     3601 cgcagcccgt ggcggccgga taccttggtc agcacgatgg agtcgccgac gggcacgccg
     3661 atgtcggcgg gggccatgat ctcaaacgtc t
//
LOCUS       DAORPG010000125         3683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2139_length_3683_cov_3.587927, whole genome shotgun sequence.
ACCESSION   DAORPG010000125 DAORPG010000000
VERSION     DAORPG010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2139_length_3683_cov_3.587927"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1360)
                     /locus_tag="PLX85_03885"
     CDS             complement(56..1360)
                     /locus_tag="PLX85_03885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC62347.1"
                     /translation="MTLSPRPAALPGSRRMFYGWYITFGGALNNFLLSAISVWGFGVF
                     IRPLRDEFGWSSAVIAAGFSIRSFQQGFLAPFVGIVIDRLGPRAMVFAGTIVLAAGFL
                     LFAVTQSIPMYFAASLLIALGQSIGSFTAFTAVLMRWFSVKRGRAIGVLNAGNGAGYF
                     LVPGIAFIVSAAGWRPALVVCAALILLIGLPIAFIVRDDPADLGLNVDGAASDASLGE
                     VDGQNRDRKELTGATVAEALHMRAFYLLAFAQAIGGGAVNVWLVHAIPHLENVGFSLG
                     AATAIGVGFAICQIAFRPLGGVLGDRIGRRRLFGVGFVFVGLGMVVFSLLTSDRLWLL
                     PLYYATFAVGQAAWVVLQAATIADYFGPRRFATISGLANLMQMPVGVVAPIIAGWAFD
                     RSGTYTPVFLVFAVGPLLSALCVALAPAPTRTVSLVASESAA"
     gene            complement(1436..1900)
                     /locus_tag="PLX85_03890"
     CDS             complement(1436..1900)
                     /locus_tag="PLX85_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HRC62348.1"
                     /translation="MVYEVDFRNVSTVGLESSPVAEALAGLRANEARYFSNKYSHVFR
                     VVPAQDGAETIDWVHRILKTERDLVIASRPLEASDFQVDNLHMAYVFYENGLSINVMY
                     ALDDQGKRAVGFKLSDGMEVPEELASRFKFARQKSKLAGTIRGSFFVIKGQY"
     gene            complement(1968..3017)
                     /locus_tag="PLX85_03895"
     CDS             complement(1968..3017)
                     /locus_tag="PLX85_03895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRC62349.1"
                     /translation="MTSLGHVPFSVLDLANVPDGSDVGTALKNSIDLARHVEALGYQR
                     FWVAEHHNMPGIASSAPGVLIAHVADATSSIRVGSGGVMLPNHAPLVVAEQFGMLEAL
                     HPGRIDLGIGRAPGTDPVTAHALRRDTDPLSAEDFPQQLGELLAFFSGRFPEGHPYRR
                     ITAVPGLGNEPAIWLLGSSDFSAQLAGWLGLPFSFAHHFSARNTMPALGAYRSNFHPS
                     ETLARPHAMVAVAVICAETDERAEWLAGSSTLSFVRLRTGTPGRLPTPEEAATHVYSP
                     TEQLVLQTRSAGLIVGGPAKVREELSRLLEETDADELMITTTVHSHEERLRSYELVAD
                     IAQVRDAPRTAVGPR"
     gene            3267..>3683
                     /locus_tag="PLX85_03900"
     CDS             3267..>3683
                     /locus_tag="PLX85_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRC62350.1"
                     /translation="MDLPAFVGLATAAAAAGAVNAIAGGGTLITFPSLLAAGFSTKVA
                     NVTSTVAIWPGTVGGSFAYRRELRARRRRVVILSGPSVLGALVGSVLLLATSQRLFDA
                     IVPWLIIFAAVLLAMNARLAAFAYAHRIGANHEEHTP"
BASE COUNT          638 a         1202 c         1273 g          570 t
ORIGIN      
        1 tcggagcacg tcccttcggc gatgctggtt cttctgtggt ccccgcctcg ccctgctatg
       61 cggcggactc gctggcgacg agcgagacgg tgcgcgtcgg cgcgggggcc agtgcgacgc
      121 acagggccga gagcagtggg ccgaccgcga acaccaagaa gacgggcgtg tacgtcccgc
      181 tccggtcgaa ggcccaccct gcgatgatgg gcgcaaccac cccgaccggc atctgcatca
      241 ggttcgcgag tccgctgatg gtggcgaaac gccgcggacc gaagtagtcc gcgatcgtcg
      301 ctgcctgcag caccacccag gcggcctggc ccactgcgaa ggtcgcgtaa tacaggggca
      361 ggagccacag ccgatcactg gtgagcagcg agaagacgac catcccgagg ccgacgaaga
      421 cgaatcccac gccgaataga cggcgtcttc cgatgcggtc gccgagcacg ccgccgagcg
      481 gccggaaggc aatctgacag atcgcgaacc ccacgccgat tgcggtcgcg gctcccagcg
      541 agaagcccac attctcgagg tgcgggatcg catgtaccag ccacacgttc accgcgccgc
      601 cgccgatcgc ctgcgcaaac gcgagcaagt agaacgcccg catgtgcagc gcctcggcaa
      661 cggtggcgcc ggtcagctcc ttgcggtccc ggttctgtcc gtcgacctcg ccgagactcg
      721 cgtcggatgc cgctccgtcg acgttcagcc ctaggtcggc cgggtcgtct cgcacgatga
      781 aggcaatcgg gagcccgatg agcaggatga gcgcggcgca aacgaccaga gcggggcgcc
      841 agccggcggc gctgacgatg aacgcaattc cgggtacgag gaagtagccc gccccgttgc
      901 cggcattgag gaccccgatg gcgcggccgc gcttcaccga gaaccagcgc atcagcaccg
      961 ccgtgaacgc ggtgaacgac ccgatgcttt gcccgagcgc gatgagcagg ctcgcagcga
     1021 agtacatcgg gatgctctgc gtcactgcga agagcaggaa gcccgccgcg agcacgatgg
     1081 tcccggcgaa gaccatcgct cgcggcccaa ggcggtcgat gacgatgccg acgaacggcg
     1141 cgaggaatcc ctgctggaag ctccggattg agaagcccgc ggcgatgact gccgagctcc
     1201 agccgaactc gtcgcgcaga ggccggatga agacgccgaa tccccatacc gagattgccg
     1261 agagcaggaa gttgttgagc gcgccaccga aggtgatgta ccagccgtag aacatgcgcc
     1321 gcgatcccgg cagcgcggcg ggccgcggcg acagcgtcat tccatcgagc ccatcgatgt
     1381 ccccttccgg gcgtgaagca gccgaccgcc tcttcagaag caatccgtcg acgcgtcagt
     1441 actggccctt gatcacgaag aatgatccgc gaatggttcc cgccagtttg gacttctgcc
     1501 tcgcgaattt gaagcgcgag gccagttctt cgggtacctc catgccatcg gagagcttga
     1561 accccaccgc gcgcttcccc tggtcgtcga gtgcgtacat gacgttgatc gagaggccgt
     1621 tctcatagaa gacatacgcc atgtggaggt tgtcgacctg gaagtcgctc gcctcgagcg
     1681 gccgtgacgc gatgaccagg tcgcgttccg tcttcaggat gcgatgaacc cagtcgatcg
     1741 tctccgcccc gtcctgcgcc ggcacgacgc gaaagacgtg gctgtacttg ttgctgaagt
     1801 agcgggcctc gttggcgcgc aagccggcga gcgcctctgc tacaggcgat gactccaatc
     1861 caacggtgga gacatttctg aagtccactt cgtacaccat cgactgctcc ctttcggcgg
     1921 aaccgagcgt tccccggcgg ccgacatgcg aaggctctgc cgtcgagcta tcgaggcccg
     1981 acggcggttc tcggggcatc tcgcacttgg gcgatgtccg ccaccaactc gtacgagcgc
     2041 aaccgctcct cgtggctgtg caccgtggtc gtgatcatga gttcgtcggc gtcagtctcc
     2101 tcgagcagcc gggacagttc ctcacggacc ttcgcgggtc cgccgacgat caggccggcg
     2161 gagcgcgtct gcagcaccag ctgctcggtc gggctgtaga catgggtcgc cgcttcctcg
     2221 ggggtcggga ggcggccggg cgtaccggta cggaggcgga cgaacgagag cgtggaggag
     2281 ccggcgagcc actccgcccg ctcatcggtt tcggcgcaga tcaccgccac cgccaccatc
     2341 gcgtgggggc gcgccagggt ctcggagggg tggaagttcg aacgataggc acccagcgct
     2401 ggcatggtgt tcctggcgct gaaatggtgc gcgaacgaga acgggagtcc gagccagccg
     2461 gcgagctgcg cgctgaagtc gctggagccg aggagccaga tcgcgggctc gttgccgagg
     2521 ccggggacag cggtgatgcg gcggtagggg tgaccctccg ggaagcggcc agagaagaac
     2581 gcgagcagtt ccccgagttg ctgcgggaag tcctcggctg acagggggtc ggtgtccctg
     2641 cggagggcat gtgccgtgac ggggtcggtc ccgggtgcgc gcccgatccc gaggtcgata
     2701 cgcccggggt ggagcgcctc cagcatgcca aactgctccg caacgaccag cggcgcgtgg
     2761 ttgggcagca tgacgccgcc cgaacccact cgaatggaac tcgtggcgtc cgccacgtgt
     2821 gcgatcagca cgccgggggc agagctcgcg atgccgggca tgttgtggtg ctcagcgacc
     2881 cagaagcgct ggtacccgag tgcctcgacg tggcgcgcga ggtcgatcga gttcttgagg
     2941 gccgtgccga cgtccgagcc gtcgggcaca ttcgcgaggt cgaggacgga gaacggcacg
     3001 tgtccgagcg atgtcattgc gcgagcttac cccaagtatc gtctgcccga tctcagagta
     3061 ggacgtccac gagcggacgg ccccatgagg acggtgctgg ccggtcagcg ggcggcggag
     3121 gggtccctcg tgcgggccgg gggaacggaa gcacggtcgc cagggcattc ggtgccgatc
     3181 gcgaacgcac cgcgcagccg ggactcctcg tagtctgatg ccgagcgacg cggcctggtc
     3241 acgccgtcga gtcatgcgga ggagtgatgg accttccagc gttcgtgggg cttgcgacgg
     3301 cagccgcggc ggccggcgcg gtgaacgcca ttgccggcgg cggcacgctg atcaccttcc
     3361 cgtcactcct cgcggcaggc ttttcgacca aagtggcgaa cgtcacgagc acggttgcga
     3421 tctggccggg gacggtcggc ggatccttcg cctatcggcg cgagctccgt gcgaggcgcc
     3481 gccgagtggt catcctcagc ggccccagtg tgctgggcgc actcgttggc tcggtgttgc
     3541 tgctggcgac ctcgcagcga ctgttcgacg caatcgtgcc ctggctgatc atcttcgccg
     3601 cggtgctgct tgccatgaat gcccgcctcg cagcgttcgc atatgcgcac cggatcggag
     3661 cgaaccacga ggagcacacg ccc
//
LOCUS       DAORPG010000126         3663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2161_length_3663_cov_3.548780, whole genome shotgun sequence.
ACCESSION   DAORPG010000126 DAORPG010000000
VERSION     DAORPG010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2161_length_3663_cov_3.548780"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="PLX85_03905"
     CDS             complement(<1..821)
                     /locus_tag="PLX85_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRC62351.1"
                     /translation="MAEANERRLPLQGVRILAQAIVWAGPFGSMILSDLGADVIEVES
                     IQHLNPTRAGMRHLPPAILEGPTGSLYLNRDGSEGFWNRQAFYNFAKRGHRSVTIDLR
                     SEDGRELFLGLIREADVFMENNAANVVEHLGIDYEQLSAVNPRLIMMRFPGFGIDGPY
                     KHFKGYGATMEAVVGHSLLRGYADSDPSGTPAVYHGDPNAGAHAAFAIQAALYARERT
                     GKGQLIDLSQAEAVTHHISYDLIDYLFNGRANQHWGNRHPAMAPYGVFPAAGEDT"
     gene            complement(822..2030)
                     /locus_tag="PLX85_03910"
     CDS             complement(822..2030)
                     /locus_tag="PLX85_03910"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRC62352.1"
                     /translation="MSGALEGIRVLDLTQGIAGPYATKLFSDYGADVLKIERPGAGDV
                     SRRMGPFPKDEPHLETSGMFLELNTGKRSVTLDLKTASGQAILRRLAEDADVVVESFR
                     PGTLERLGLGPDVLETVNPKAALVRVSNFGQYGPYRDYNADDMLLYGMGGVLAVTATE
                     GREPVRIGLYAPLFLAGGVIAAFTMGAFNGSRASGVGERADISIHEILTASMDRGGPN
                     LVAYQYSGSLFFERGRASRASAMPNGVYPCADGYLHITTGPNWWDRFCRAIDRPDLIT
                     DERLLPHLLDPEWAPEIDAIIYPWLLERTKQEIMERAQAEGWVVAAINGMDDVARDPH
                     LEARGFWAHLDHPMAGNVTLPGFPFRMLGTPGEIRRAPLLGEHTSSVLAEQLGYSAED
                     IARLAQRGVI"
     gene            2231..>3663
                     /locus_tag="PLX85_03915"
     CDS             2231..>3663
                     /locus_tag="PLX85_03915"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC62353.1"
                     /translation="MSAGAPTTPAPTRNRHLIFALLIFSLLTVVMQFTMVSVSVKQLT
                     EDLNAPLRWSSWVITIFALGQVIAQPVAGRLTERMGAKMVFAGGLAIFGVASLVCAAA
                     PNVYVMIVARFIQGVAGGGVLPAGSAIIGDVYGENRAKAIGFFASLTPFASVLGPTVG
                     GLIVDTIGWRWTFGINGPLSIVACALGFALLPRGRRVDGIKVDYAGVVLIAAAATPII
                     FALTELGRRGVTPNYAIVAGAAAVGLTALAVLIWHERRTAVPVVDMDLVTRVEFLSAN
                     GLMFFFGLCWGSMTLLMPLFLQETYGFSASEAGALLAPRSVAMMASSTLFATLLMRTG
                     YRRPLAVGFLAVAALIFLMAQGFEGPNVLGLQLNNFWYVMAIVLGLGLVFGVSNPSMN
                     NAGFDIAPDRIAAIAGLRMMAQQLGSLIGIAVAVLIASRAATVSGGLTIAFSIFSVLL
                     ACSTVMVLRVPEIRTAGAPATRREPRAA"
BASE COUNT          518 a         1381 c         1128 g          636 t
ORIGIN      
        1 aaggtgtcct cgccggctgc ggggaagacg ccatacggcg ccatcgccgg gtgccggttg
       61 ccccagtgct ggttcgctcg tccgttgaac aggtagtcga tcaggtcgta cgagatgtgg
      121 tgcgtgaccg cctcggcctg tgacaggtcg atcagttggc ccttgcccgt gcgctcgcgc
      181 gcgtacagcg cggcctggat cgcgaacgcg gcgtgcgccc cggcattcgg gtcgccgtga
      241 tacaccgccg gcgtccccga cgggtccgag tcggcgtacc cgcgcagcag cgagtgtccg
      301 acgaccgcct ccatcgttgc cccgtagccc ttgaagtgtt tgtacgggcc gtcgatgccg
      361 aaacccggga accgcatcat gatcagccgc gggttcacgg ccgacagctg ctcgtaatcg
      421 atcccgaggt gctcgacaac gttcgcggcg ttgttctcca tgaacacatc cgcctctcgg
      481 atgagtccaa ggaacagctc ccgcccgtcc tcgctgcgga ggtcgatcgt cacgctccgg
      541 tgtccgcgct tcgcgaagtt gtagaaggcc tgccggttcc agaacccctc gctgccgtcc
      601 cggttgaggt acagcgaccc cgtcgggcct tcgaggatcg cgggcggcag atgccgcatg
      661 cccgcgcgcg tcgggttcaa atgctgaatc gactccacct cgatcacgtc cgcgccgagg
      721 tccgacagga tcatcgagcc aaatgggccc gcccagacga tcgcctgcgc caggatgcgc
      781 acgccctgca gcggcagccg ccgctcgttc gcctccgcca tctagatcac cccccgctgc
      841 gccagccgcg cgatgtcctc cgccgagtac cccagctgct ccgccagcac gctcgaggtg
      901 tgctcaccga gcagcggcgc gcgacggatc tcccccggcg tacccagcat ccgaaatggg
      961 aagcccggca acgtgacgtt tcctgccatc gggtggtcga ggtgcgccca gaagccgcgc
     1021 gcctccaggt gtgggtcgcg tgcgacgtcg tccatgccgt tgatcgcggc cacgacccaa
     1081 ccctcggcct gcgcgcgttc catgatctcc tgcttcgtcc gctccaggag ccacgggtag
     1141 atgatcgcgt cgatctccgg cgcccactcc gggtcgagga gatgcggcag cagccgctcg
     1201 tcggtgatca ggtcgggccg gtcgatcgcg cggcagaagc ggtcccacca attcggcccg
     1261 gtcgtgatgt gcaggtagcc atccgcgcac gggtagaccc cgttcggcat cgccgaagcc
     1321 ctcgaggcgc gcccgcgctc gaagaacagc gaccccgagt actggtaggc gacgaggttg
     1381 ggccccccgc ggtccatcga ggcggtcagg atctcgtgga tggagatgtc cgcccgctcc
     1441 cccacgcccg aggcacgcga cccgttgaac gcccccatcg tgaacgcggc gatgacgccg
     1501 ccagcgagga acagcggcgc gtacagcccg atgcgcaccg gctcgcgccc ctcggtcgcg
     1561 gtcaccgcca gcacgccgcc catgccgtac agcagcatgt cgtcggcgtt gtagtcgcgg
     1621 tacggtccgt attgcccgaa gttcgagacc cggacgagcg ccgccttcgg gttcaccgtc
     1681 tcgaggacat ccggccctag gccgagccgc tcgagggtcc ccggacggaa gctttcgacg
     1741 accacgtcgg cgtcctcggc gagccgccgc aggatcgcct gccccgacgc ggtcttgagg
     1801 tcgagcgtga cgctccgctt ccccgtgttc agctcgagga acatcccgct cgtctcgagg
     1861 tgcggctcat ccttcgggaa cggccccatc cgccttgaga cgtcacccgc cccgggccgc
     1921 tcgatcttca agacatcggc gccgtagtcc gagaacagct tcgtcgcgta cggccccgcg
     1981 atcccctgcg tcaggtcgag gacacggatg ccctcgagcg ctccgctcat gattccccct
     2041 cattcggtcg aaaacggtgc ggcgatgata aacgcaagtc gcgctcgccg caggacatcc
     2101 gccgcgcagc ttgaacgccg cgatacccta cgcgcgacga ggtggggcat tccttaagtg
     2161 aaggttgatt gaaccgcgac gcgccactac cgtcgtagtc agacgcgtcc cacgccgttc
     2221 cggagcgacc atgtcagcgg gtgcaccgac gacgccggcc ccgacccgga atcgtcacct
     2281 gattttcgcc ctcctcatct tctcgctgct gaccgtcgtc atgcagttca cgatggtctc
     2341 tgtctcggtg aagcagctca ccgaggacct gaacgcgccc ctccgctgga gctcctgggt
     2401 catcacgatc ttcgccctcg gccaggtgat cgcgcagccg gtcgccggcc gcctcaccga
     2461 gcgcatgggc gccaagatgg tctttgccgg cggcctcgcc atcttcggcg tcgcctcgct
     2521 cgtctgcgcc gccgcgccca atgtctacgt catgatcgtc gcccgcttca tccagggcgt
     2581 cgccggcggc ggcgtccttc ccgccggctc cgccatcatc ggcgacgtct acggcgagaa
     2641 ccgagcgaag gcgatcggct tcttcgcctc cctcaccccc ttcgcgagcg tcctcggccc
     2701 gaccgtcggc ggcctcatcg tcgacacgat cggctggcgc tggaccttcg gcatcaacgg
     2761 tccgctctcc atcgtcgcct gcgctcttgg cttcgccctc ctgccgcgcg gccgccgtgt
     2821 cgatggcatc aaggtggact atgccggcgt cgtgctgatc gccgccgctg cgacgccgat
     2881 catcttcgcc ctcaccgagc tcggccgccg aggtgtcacc ccgaactacg ccatcgtcgc
     2941 gggcgccgca gccgtcggac tgaccgctct cgccgtgctc atctggcacg agcgccgcac
     3001 ggcggtgccc gtcgtcgaca tggacctcgt cacccgcgtc gagttcctct ccgccaacgg
     3061 tctgatgttc ttcttcggcc tctgctgggg cagcatgacg ctcctcatgc cgctcttcct
     3121 gcaggagacc tacggcttca gcgcctccga ggccggtgcg ctcctcgctc cgcgctcggt
     3181 cgcgatgatg gcgtcatcga cactcttcgc cacgctgctc atgcgcaccg gctaccgccg
     3241 cccgctcgcg gtcggcttcc tggccgtcgc cgcgctcatc ttcctcatgg cccagggctt
     3301 cgagggccca aacgtcctcg gcctgcagct caacaacttc tggtacgtga tggcgatcgt
     3361 gctcgggctc ggcctcgtct tcggcgtctc gaacccctcg atgaacaacg ccggcttcga
     3421 tatcgccccc gaccgcatcg cggccatcgc gggactccgc atgatggcgc agcagctcgg
     3481 cagcctgatc ggcatcgccg tcgccgtact catcgcctcg agggccgcga ccgtctcggg
     3541 cggcctcacc atagccttct cgatcttctc ggtgctgctc gcctgcagca cggtcatggt
     3601 gctgcgcgtc cccgagatcc gcaccgccgg cgcgccggcc acccgtcgcg agccgcgcgc
     3661 cgc
//
LOCUS       DAORPG010000127         3632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2194_length_3632_cov_3.594632, whole genome shotgun sequence.
ACCESSION   DAORPG010000127 DAORPG010000000
VERSION     DAORPG010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2194_length_3632_cov_3.594632"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..1123)
                     /locus_tag="PLX85_03920"
     CDS             complement(188..1123)
                     /locus_tag="PLX85_03920"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HRC62354.1"
                     /translation="MPTPTAFFQGDFVPLEQAQIPVTTHAFNYGTAVFEGIRGNWNGE
                     QNELYLFRLREHIQRLRQNAKILRIGIRYSDEELIRIVTDLALRNDYREDVYIRPIAY
                     KSQSVIGPRLHNVEDDVLFYLIPLGAYLDSDSGITCTVSSWRRVDDNMIPARAKVAGL
                     YVNNSLAKTEAQQNGFDEAILLNHDGHVSEGSGENIIIVRGGKLISPPPADNILEGIT
                     LDTAFEIARSLGYETEQRTIDRTELYIADEIFMTGTAAHVTPVLSVDRVPLNEGKPGP
                     ITTAIQRHFFEAITGRLPQYASWLTPVYAGAAVTA"
     gene            complement(1238..1918)
                     /locus_tag="PLX85_03925"
     CDS             complement(1238..1918)
                     /locus_tag="PLX85_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRC62355.1"
                     /translation="MTNAERVSTVEYPDIFDAIMADDLEAIEAMLHVDPALAARQDHR
                     SPILLAAYRQRRDALEALLRREPPLDVFEAAATGALDVVRVLLAERPGRVGAYSDDGL
                     TALHLAAFFGHDPVVQELIDRGARPGAVAHNEEESAPLHLAVAGAHQSTAALLLELGA
                     AVDAQELGGVTPLHLAARRGNFDLVRLLLSHGARREVETETGKTAADLAYEFEHDALA
                     AFLRSGGG"
     gene            2012..2839
                     /gene="uppP"
                     /locus_tag="PLX85_03930"
     CDS             2012..2839
                     /gene="uppP"
                     /locus_tag="PLX85_03930"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HRC62356.1"
                     /translation="MDDYLRAAVLGLVQALTEFLPISSSGHLVLAGHILGERANSLTF
                     DVGLHVGTLIAVLAYFWREWVAIVFGGLRDILRHGPRIDRWQPHSRLGLWIVLGTLPA
                     VVLGALFDNAIEEHLRQAWLVASLLIVFALVLEVADRAAARRHDLEAVGPANALAIGL
                     AQAVALVPGVSRSGATISAARGLGFDRAVAARFSFLLSAPAVLGAATLKLTDAVRGTE
                     DVSWGPMALGALVSAVAGAAVIHWLLGYLQSHGLRPFVWYRLAAGVAVLAVAAVSTR"
     gene            2842..3552
                     /locus_tag="PLX85_03935"
     CDS             2842..3552
                     /locus_tag="PLX85_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC62357.1"
                     /translation="MSAEESGRSFLGRRGLVIGVETDAGSAVASALGTAGADLALATM
                     RADEGVLVARRLQRQLRELGRQASTYAFDVTLGQNVKVSTRQVAKELGGLDFVVSAPD
                     EPLVGPFERLTDSDLARVTTVNFYAHVYAVRAALDEFRRGGEGHALLVVRRSEDGGAA
                     YQAAQAATLSLVTTLADELRERAVTISALIVPANIDGARAGRAALELLQGDPARASGR
                     VYDLAARPEGAADHEGRP"
BASE COUNT          485 a         1289 c         1267 g          591 t
ORIGIN      
        1 catcatgctc gatgcctcgc gcgcccgcga agtgtggggt tggcagcccc agaccgacct
       61 cgagaccggg cttgccgcca ccgtccgctg gttccgcgag acgagcggct gacgggacgc
      121 cccggcagcg acgacgaaag agccgccgga aaccggcggc tctttgctct gaccgtggcg
      181 acgagggcta ggccgtgacg gcggcccccg cgtagacggg cgtcagccac gaggcgtact
      241 gaggcaggcg ccccgtgatc gcctcgaaga agtggcgctg gatcgcggtg gtaatcggac
      301 ctggctttcc ctcgttcagg ggcacgcggt cgacggagag caccggcgtc acgtgcgcgg
      361 cggtccctgt catgaagatc tcgtccgcga tgtacagctc ggtgcggtcg atcgtgcgct
      421 gctcggtctc gtagccgagc gagcgcgcga tttcgaacgc cgtgtccagc gtgatgccct
      481 caaggatgtt gtccgccggc ggcgggctga tcagcttgcc gccgcgcacg atgatgatgt
      541 tctcgcccga cccttccgag acgtgcccgt cgtggttgag caggatcgcc tcatcgaagc
      601 cgttctgctg cgcctccgtc ttcgcgagcg agttgttcac gtacaggccg gcgaccttgg
      661 cgcgcgccgg gatcatgttg tcgtccacgc gtcgccacga ggacaccgtg cacgtgatcc
      721 ccgagtccga gtcgaggtag gcgccgagcg ggatcagata gaacagcacg tcgtcctcga
      781 cattgtgcag tcgagggccg atgacgctct gcgacttgta cgcgatcggc cggatgtaca
      841 catcctcgcg gtagtcgttg cgcagggcca gatccgtgac gatgcggatc aactcctcat
      901 cgctgtagcg gatcccgatg cgcaggatct tcgcgttctg gcgcagccgc tggatgtgct
      961 cgcgcagccg gaacaggtac agctcgttct gctcgccgtt ccagttgcct cgaatgccct
     1021 cgaataccgc ggtcccgtag ttgaaggcgt gggtcgtgac cgggatctgg gcctgctcca
     1081 gcggcacgaa gtcgccctga aagaacgcgg taggtgtggg catacggacc tccgtttcga
     1141 cggcctggcc ggaattcggt acgggttgca ggccgggatg taggtgtcag cgatgatagt
     1201 gctggccgtg atacgcgagc aaggccggcg gacgcgctca ccctccgccc gagcgcagga
     1261 acgccgccag cgcgtcgtgc tcgaactcat acgcgaggtc cgcggccgtc ttccccgtct
     1321 cggtttcgac ctcgcgacgc gcgccgtgcg acagcagcag ccgcacgagg tcgaagttgc
     1381 cgcgccgtgc cgccaggtgc agcggcgtca ccccgcccag ctcctgggcg tctaccgcgg
     1441 cgccgagttc gagcagcaac gcggcggtgc tctggtgcgc tccggcgacc gcgaggtgga
     1501 gcggcgcgct ctcctcctca ttgtgggcga ccgcgccggg ccgggctccg cggtcgatca
     1561 gctcctgcac caccgggtcg tggccgaaga acgccgcgag gtgcagcgcg gtcagtccat
     1621 cgtcggaata ggccccgacg cgtccggggc gctcggccag cagcacgcgc acgacatcga
     1681 gggcgcccgt ggcggcggcc tcgaagacat cgagtggtgg ctctcgccgc agcagcgcct
     1741 cgagggcatc gcgccgctgg cgatatgccg cgagcaagat aggcgagcgg tgatcctgtc
     1801 gtgctgccag cgccgggtcg acgtgcagca tcgcctcgat cgcttcgagg tcgtccgcca
     1861 tgatcgcgtc gaagatgtcc gggtactcga ccgtggagac ccgctcggcg ttcgtcacgc
     1921 ttccctccgc cgtccgatgc tagacgagcg gacaccgaga ggcgaccgtc gcaggctggc
     1981 ccgctgcacc gccccggcgt agcctcgccg tatggatgac tacctccgcg cggcggtgct
     2041 cggtctcgtg caggcgctca cggagttcct ccccatctcg tcgtcgggtc acctcgtgct
     2101 cgcgggccac atcctcggcg agcgcgccaa ctcgctgacc ttcgacgtcg ggctccacgt
     2161 cgggactctg atcgcggtgc tggcgtattt ctggcgcgag tgggtcgcca tcgtcttcgg
     2221 cgggctccgc gacatcctgc gccatgggcc acgcatcgac cgctggcagc cccattcccg
     2281 gctcgggctc tggatcgtcc tcggcacgct gcccgccgtg gtcctcggcg cgctgttcga
     2341 caacgccatc gaggagcacc tgcgccaggc ctggctcgtg gcatcgctgc tgatcgtgtt
     2401 cgcgctcgtc ctcgaagtcg ccgaccgcgc ggcggcgcgt cgccacgatc tcgaggcggt
     2461 cgggccggcc aacgcgctcg ccattggcct cgcccaggca gtcgcgctcg tgcccggcgt
     2521 gtcgaggtcg ggcgccacca tcagcgcggc gcgcggtctc ggcttcgacc gcgctgtagc
     2581 cgcgcgcttc tccttcctgc tctcggcccc ggcggtgctc ggcgcggcca cgctcaagct
     2641 cacggacgcc gtgcgcggca ccgaggacgt gagctggggg ccgatggccc tcggcgccct
     2701 cgtctccgcc gtcgccggcg ccgcggtcat ccactggctg ctcggctacc tgcagtccca
     2761 cggcctccgg ccgttcgtgt ggtaccgcct ggccgccggc gtcgccgtgc ttgccgtggc
     2821 ggctgtgagc acccgctgaa cgtgtcagcc gaggagtcag ggcgctcgtt cctcggacgc
     2881 cgcggcctgg tgattggcgt tgagacggac gccggctccg ctgtcgcctc ggcgctcggg
     2941 acggcgggcg cggacctcgc gctcgccacg atgcgtgccg acgagggcgt gctcgtcgcg
     3001 cgccgcctgc agcgccagct acgcgaactc gggcgccagg cgagcacgta cgccttcgac
     3061 gtgacgctcg gacagaacgt gaaagtctcc acccggcagg tcgcgaagga actcggcggc
     3121 ctcgacttcg tcgtgagcgc gcccgacgaa ccgctcgtcg gcccgtttga gcgcctgacg
     3181 gactccgacc tcgcccgcgt cacgaccgtc aacttctacg cgcacgtcta tgccgtgcgc
     3241 gcggcgctcg acgaattccg gcgcggcggt gaggggcacg cgctcctcgt cgtgcgacgg
     3301 tccgaggacg gcggggcggc ctatcaggca gcgcaggcgg ccacgctgag cctcgtgacc
     3361 acgctggccg atgagctccg cgaacgggca gtcaccatca gcgcgctcat cgtgccggcc
     3421 aatatcgacg gcgcgcgcgc gggtcgtgcg gcgctcgaac tgctgcaggg cgatccggcg
     3481 cgtgccagcg gccgggtcta tgatctggcg gcgcgtcctg aaggcgcggc agaccacgaa
     3541 gggcgaccat gacctccagc acgtccgctc ggcccctcga tggcctgcac gccctcgtcg
     3601 cgggcgcggg ggggccgcac gcccgccctg cc
//
LOCUS       DAORPG010000128         3615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2217_length_3615_cov_3.765449, whole genome shotgun sequence.
ACCESSION   DAORPG010000128 DAORPG010000000
VERSION     DAORPG010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2217_length_3615_cov_3.765449"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..550)
                     /locus_tag="PLX85_03940"
     CDS             complement(83..550)
                     /locus_tag="PLX85_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HRC62358.1"
                     /translation="MTTEQPVFEYDRDIIGKQFELGSQEITRERIRRFCEVLGETNPL
                     FTDDEAAASGPYGAVIAPPGLVYSLQLGQGPDAKVVFGQATFHAGERAELHDVVRPGD
                     VVTARQQIKDVFAKTGRSGTMVFVVRRTEFTNQHGTLVAAVEQSMVHREVNRG"
     gene            complement(631..1914)
                     /locus_tag="PLX85_03945"
     CDS             complement(631..1914)
                     /locus_tag="PLX85_03945"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC62359.1"
                     /translation="MLGTRRRNGASAAAVALPAPRPAPRFGAAFESLHMRDFRFLFFS
                     TVSAGYAQWAQSIGIGWLAYQLTGGSALQLAAVSATGGVVRLVVSPFVGAALDRYNRR
                     QVLVSSTLLSALQGSLLAALVVSGYAHVLHLYIFMVLDGIFSTTNQAARQAYVYDITT
                     NETMPNAMALSAIAQNLSRISGPPLAAVMIGFMGRSSPFVVLAVLHVLAAVLTLAISR
                     RSRQAARVSIHPLRGPLEGFVYVRREHALLGLMVIGTVPALLVYPYVQLLPVFAKDVL
                     DGDAITFGLLAAAIGWGSIVGLLGLAFVASSVKRRGTIAIWGMVGYAIALIAFSQSTT
                     LLAALLLLTIAGVFHGVSLNLQQMLVQMLARDDMRGRTTSLFQMGFALMPIGVLPMGA
                     AIDRWGAAHALGAFFLVSAVVFVLMGLFWRSLREV"
     gene            complement(2089..2985)
                     /locus_tag="PLX85_03950"
     CDS             complement(2089..2985)
                     /locus_tag="PLX85_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRC62360.1"
                     /translation="MDYRFVTSETLDDGAIVRVMLNRPDFRNAQSRGLLVELDDALLR
                     AEADDRVRVIILGGAGPTFSAGHDMGTPESIAERTRGGPDEHPTVRGYGGTYAGLESR
                     MRQEWHFFFQNTLRWRNLRKVTIAQVQGKCYAAALMLAWACDLIVASEDAEFADVVGT
                     RLGMCGVEYFAHPWEFGARKTKELLLTGDSLGAEEAHRLGMVSKVFPRERLEADTLEF
                     ARRIAKLPTVTALLVKESVNQTLDNQGFYNALQACFTLHQANHSHWAEVHDDHMPIGS
                     PEDGIAHWSVAPPPAPLSMTAP"
     gene            3012..>3615
                     /locus_tag="PLX85_03955"
     CDS             3012..>3615
                     /locus_tag="PLX85_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62361.1"
                     /translation="MRAHCTPRSPALGRPCVEERPQGRVDWRRLPRDERAQLSAPTAG
                     RTLTPARAQYFALKQQHPDALLLFRMGDFYETFDADAETVAAVTGVALTSRPMGKSEG
                     RVPLAGVPYHALERYLDQLVAAGHRVAIVEQVSPPGRGLVERRVVRVVTPGTVEAGAL
                     LEERGHNWLVALAPEGPAEARWGLAACDVTTGEFVCQLVSG"
BASE COUNT          574 a         1259 c         1254 g          528 t
ORIGIN      
        1 gtacgcggcg acctgtgggg tctccgccgt ccactgctcc tcgaactcgt cacccgggtc
       61 gacctgctcg aagtagcgct gctcaaccac ggttgacctc ccggtgcacc atcgactgct
      121 ccacggccgc gaccagcgtg ccgtgctggt tggtgaactc ggtgcggcgc accacgaaga
      181 ccatcgtccc cgagcggccg gtcttggcga acacgtcctt gatctgctgg cgtgccgtga
      241 cgacgtcgcc cgggcgcacg acgtcatgca gctcggcgcg ttcgcccgcg tggaaggttg
      301 cctgcccgaa caccaccttg gcgtcggggc cctgcccgag ttgcagcgag tacacgaggc
      361 cggggggcgc gatcaccgcg ccgtacggcc cggaggccgc agcctcatcg tccgtgaaga
      421 gcgggttcgt ctcgcccagc acctcgcaga accgccggat gcgctcccgc gtgatctctt
      481 gcgagcccag ctcaaactgc ttcccgatga tgtcccggtc gtactcgaag accggttgct
      541 ccgtcgtcat cgtccctcct tttcgtcgtg gtggagcctc gccagcggcg cgggctgtgt
      601 caagcggagg cggcccccgc cgggcagccg tcagacctcg cgcaacgagc gccagaatag
      661 tcccatcagc acgaagacga ccgccgagac caggaagaag gcgccgagcg cgtgcgcggc
      721 cccccagcgg tcgatcgcgg cccccatcgg caggacgccg atcggcatca gcgcgaagcc
      781 catctggaac agactcgtcg tgcgcccacg catgtcgtcc cgcgcgagca tctgcacgag
      841 catctgctgc agattgagcg agacgccgtg gaagacgccc gcgatcgtga gcagcaacag
      901 cgccgcgagc agggtcgtcg actggctgaa ggcgatcagc gcgatggcgt agccaaccat
      961 gccccagatc gcgatcgtgc cgcggcgctt gacgctgctg gcaacgaatg cgaggccgag
     1021 caacccgacg atggagcccc agccgatggc cgccgccagc aggccgaacg tgatcgcgtc
     1081 gccgtcgagg acgtctttcg cgaacaccgg cagcagctgt acgtacgggt agacgagcag
     1141 cgccggcaca gtcccgatca ccatcaggcc gagcaacgcg tgctcccggc gcacatatac
     1201 gaagccctcg agcggcccgc gcagcgggtg gatcgacacc cgcgctgcct gacggctgcg
     1261 gcggctgatt gcgagcgtca gcaccgcggc cagcacgtgc aggacggcca gcaccacgaa
     1321 cggcgatgag cggcccatga agccgatcat gaccgccgcc agcggtggtc ccgagatgcg
     1381 cgagaggttc tgggcgatcg cgctcaacgc catcgcgttc ggcatcgtct cgttggtggt
     1441 gatgtcgtag acgtacgcct gccgcgccgc ctggttcgtc gtcgagaaga tgccgtcgag
     1501 caccatgaag atgtacaggt gcaggacgtg cgcgtacccg ctcaccacca gcgccgccag
     1561 cagggagccc tgcagcgcgc tcagcagcgt cgaactgacg agcacctgac gccggttgta
     1621 gcggtcgagt gccgccccca cgaacgggct gaccaccagc cgcacgacgc cgcccgtggc
     1681 ggagacggcc gccagctgca acgccgagcc gcccgtcagc tgatacgcca gccaaccgat
     1741 gccgatgctc tgcgcccact gcgcgtaacc cgcgctcact gtgctgaaga agaggaaccg
     1801 gaagtcgcgc atgtgcagcg actcgaaggc ggcgccgaag cgcggcgcgg gtctgggggc
     1861 ggggagggcg actgcggcgg cggaggcgcc gttgcgcctg cgggttccga gcatatgtgg
     1921 gacgcctttc gatctccgcc gggaccccgg tcggtgccga agcagcgcca ttatggcggg
     1981 tggcgccgct gtttcgctac cacgattgtt gccctgccgc caagcgccgg atgagcggac
     2041 acgacgaggc cgcgggtgat tccgcggcct cggggtatcg ggcgctcgct acggggcggt
     2101 catcgagagg ggcgccgggg gcggggcgac ggaccagtgc gcgatgccgt cctcgggcga
     2161 cccgatcggc atgtggtcgt catggacctc ggcccagtgc gaatggttgg cctggtgcag
     2221 cgtgaagcag gcctgcagcg cgttgtagaa gccctggttg tcgagggtct ggttcaccga
     2281 ctccttcacg agtagcgcgg tgacggtcgg cagcttcgcg atgcggcggg cgaactcgag
     2341 cgtgtccgcc tcgagccgtt cgcgcgggaa gactttgctc accatgccga gtcggtgcgc
     2401 ctcctcggcg cccagcgagt cgccagtaag cagcagctcc ttcgtcttgc gtgcgccgaa
     2461 ctcccaggga tgcgcgaagt actcgacccc gcacatgccg aggcgcgtgc cgacgacatc
     2521 ggcgaactcg gcatcctcgg aggcgacgat caggtcgcag gcccaggcga gcatgagcgc
     2581 ggccgcgtag cacttgccct gcacctgcgc gatcgtgacc ttgcgcaggt ttcgccagcg
     2641 cagcgtgttc tgaaagaaga agtgccattc ctggcgcatc cgcgactcga ggccagcata
     2701 ggtgccgccg tagccgcgca ccgtcgggtg ctcgtccggt ccgccgcggg tgcgctcggc
     2761 gatcgactcc ggcgtgccca tgtcatggcc ggccgagaac gtcggccccg cgccgccgag
     2821 gatgatcacg cgcacgcggt catccgcctc ggcccgcagc agcgcgtcat ccagctcgac
     2881 caacaggccg cggctctgcg cgttgcggaa gtccggacgg ttgagcatca cgcgcacgat
     2941 cgcgccgtcg tcgagcgtct ctgaggtgac aaagcggtag tccatggcat tccatccctt
     3001 cgccaagcgt catgcgagcg cattgtacgc cgcggtcgcc ggcgctcgga cggccgtgcg
     3061 ttgaggagcg tccgcaggga cgggtagact ggaggcgtct ccccagggac gagagggcgc
     3121 agctgagcgc tcccactgcc ggacgcaccc tcacgcccgc gcgggcacag tacttcgcgc
     3181 tcaaacaaca gcaccccgac gcgctgctgc tcttccgcat gggcgacttc tacgagacgt
     3241 tcgacgcgga tgccgagacc gtcgccgccg tgacgggagt ggcgctgacg tcgcgcccca
     3301 tgggcaagag cgaggggcgg gtgccgctgg ccggcgtccc ctatcacgcc ctcgagcgct
     3361 acctggacca actcgtcgcc gccggccacc gtgtcgcgat cgtggagcag gtcagcccgc
     3421 cgggccgtgg cctcgtggag cgccgcgtcg tgcgggtcgt caccccgggc acggtcgaag
     3481 ccggagcgct gctcgaggaa cgcgggcaca actggctcgt cgcactggcg ccggaagggc
     3541 ccgctgaggc gcgttggggg ctcgcggcct gcgacgtcac cacgggcgag ttcgtctgcc
     3601 agctcgtcag cgggg
//
LOCUS       DAORPG010000129         3601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2235_length_3601_cov_3.411450, whole genome shotgun sequence.
ACCESSION   DAORPG010000129 DAORPG010000000
VERSION     DAORPG010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2235_length_3601_cov_3.411450"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PLX85_03960"
     CDS             complement(<1..601)
                     /locus_tag="PLX85_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62362.1"
                     /translation="MSAIRKLLVSTATVMALVAVVSTTVISLTSPAEATKPGDDGEHK
                     VRICHRTNSVTNPYVSIEVDVAAADGSGDKGDHYSEHQGPLASSESVAQQFKDDKIEW
                     GDIIPPIEGAHDGLNWTAEGQAIWENDCNISEGTPTGTPISTPQTTPTGTPTGTATST
                     PTSTPTSTPTSTPTSTPTSTPTSQVTPTPTTTPTSTPTTT"
     gene            complement(1041..2993)
                     /locus_tag="PLX85_03965"
     CDS             complement(1041..2993)
                     /locus_tag="PLX85_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC62363.1"
                     /translation="MGMWGGGGAAGWSTGIGGQSMGPGRAAGRGSDGWDDEYLGKAYD
                     AEVVRRILPYLRPYRLQATIAFLAMVLSAAMNFAQPLIIGFAVTAGVRRDERQVVILL
                     IVLAVMAVLYWLASFAQQLAVNWLGNRLLVKLRTDMYDHMQSLSLSFYDEMEVGRMIS
                     RLTSDVTVIQDLLTSGSLTFAADIVGLGIIVIVLLVRDWQLALVTFAVVPPLVLVMVW
                     WSRHAKDAFVNVRVQVSQLYGTLAENLSGVRAVQSMSREDENSRRFDQLNRQNRAANI
                     RAAVMTAAVMPALELAVAIATAAVLIVGGLRVLNSTDADLAVFFGVLTSFTLYVGRFF
                     DPIRDLVLQYTMLQRAMAGGERVFEVLDTPPRIQDRPGALELETVEGRVDFDHVSFHY
                     VEGIPVLEDIDLHVAPGETVAFVGHTGAGKTTITSLVSRGYEVTSGAIRIDGHDLRDI
                     KRRSLTRHMGVVLQNPYLFSGTIRENIAYGDPDAPIERIQEAARAVGADDFISRLPKG
                     YDTVLQQRGQNFSVGQRQLISFARAILAAPRILVLDEATAYVDTQTEAIIQRALRELL
                     KGRTSFVIAHRLSTIREADRIVVLDRGRIAEIGTHEQLLAADGIYANLYRMTFETEQA
                     ARAAELIGEDEATARRARGELIGEPG"
     gene            complement(2993..>3601)
                     /locus_tag="PLX85_03970"
     CDS             complement(2993..>3601)
                     /locus_tag="PLX85_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC62364.1"
                     /translation="VTIESLRRNIGIVQQDVFLFIGTIRDNIAYGRPGASQEEIERAA
                     KAARIHDFIVSLPLGYDEWVGERGVTLSGGQKQRIAIARTLLLDPRILIFDDSTASVD
                     AQTEYLILQALQELMRGRTTFVIAQRLRTVMRADEIVVLDRGRIVQRGSHAQLLAQEG
                     LYRTIFDLELKDQEEALGEYRAAHQNGSGATPLPARPAEVTG"
BASE COUNT          572 a         1036 c         1390 g          603 t
ORIGIN      
        1 gagtcgtggt tggcgtcgat gtcggggtcg tggttggcgt cggggtgacc tgcgacgtgg
       61 gggtgctggt cggcgtcgaa gtcggagtgg acgttggcgt cgaagtcggc gtgctcgtcg
      121 gggtcgacgt cgcggtcccg gtcggcgtac ccgtcggcgt ggtctggggg gtgctgattg
      181 gcgtgccggt cggcgtgccc tcagagatgt tgcagtcgtt ctcccagatc gcctggccct
      241 ctgcagtcca gttcaggccg tcatgagctc cctcgatcgg ggggatgatg tcgccccact
      301 cgatcttgtc gtccttgaac tgctgagcga cggactcgct gctcgcgagc gggccctggt
      361 gctcgctgta gtggtcgccc ttgtcaccgc tgccgtcggc tgccgcgaca tcgacctcga
      421 tgctgacgta ggggttggtg acggagttgg tgcgatggca gatgcggacc ttgtgctcgc
      481 catcgtcacc cggcttggtc gcctcggcgg ggctggtgag cgagatcacc gtcgtggaga
      541 cgacggcgac gagcgccatg accgtggcag tcgagacgag gagtttcctg attgcggaca
      601 tcgtcgaacc tttccggcct cctcgggccg aagctcgccg cgggcgtgac atggcaacgc
      661 cgagcagcga ggcgcgcagt gatcaaggcg cgatgaaagg cgcaaacgag gcacaaggag
      721 tcagtcggtg tgttgacccg cacgcaaatg gcgcagcgtg cacagccggt caaccaaggc
      781 gcgaccgcga ggcgcggtca gactgagaaa cgacgtccgg gctgagggct acccgggagg
      841 ctcgtcgacg ggttcgggcg gggggaggga ggcgaccctc cagggtgagg cagcccgacc
      901 gacgcgacca gagcgaagca aacgctgacg cgaccctcga atagtacctt aacgtcgcgt
      961 tggggtgtca agcccgcatt gcgaattctg ggaggactct tacgacttaa tcggagatcg
     1021 gcgattgatt gcgaatgcga ttaaccgggt tctccgatta gctcgcctcg ggcgcgtctt
     1081 gcggtggctt cgtcttcgcc gatgagctcg gcggcgcgtg cggcctgctc ggtctcgaag
     1141 gtcatgcggt agaggttggc gtagatgccg tcggcggcaa ggagctgctc gtgcgtgccg
     1201 atctccgcga tacgcccgcg gtcgaggacc acgatgcggt ccgcctcgcg gatggtggag
     1261 aggcgatggg cgatgacgaa ggaggtccgg cccttgagca gttcgcggag ggcgcgctgg
     1321 atgatcgcct ccgtctgggt gtcgacgtag gcggtggcct catcgagcac caggatgcgg
     1381 ggggcggcga ggatggcacg cgcaaaactg atgagctgcc gctggccgac ggagaagttc
     1441 tgcccacgct gctggaggac ggtgtcgtag ccctttggca ggcgcgagat gaagtcgtcc
     1501 gcgccgaccg cccgcgcggc ttcctgaatg cgctcgatgg gggcgtccgg gtcgccgtag
     1561 gcgatgttct cccggatcgt gccgctgaaa aggtacgggt tctgcagcac gacgcccatg
     1621 tggcgcgtga gtgagcgacg cttgatgtcc cgcaggtcgt ggccatcgat gcggattgcg
     1681 ccgctggtga cctcgtagcc acggctgacg agcgaggtga tcgtggtctt gccggcgccg
     1741 gtgtgtccga cgaaggcgac ggtctcgccg ggggcgacgt ggaggtcgat gtcctcgagg
     1801 acgggaatgc cctcgacgta gtggaaggag acgtggtcga agtcgacgcg gccctcgacg
     1861 gtctcaagct cgagcgcgcc ggggcggtcc tggatgcggg gtggggtgtc gaggacctca
     1921 aagacgcgct cgccgccggc catggcgcgc tgcagcatcg tgtactggag gacgaggtcg
     1981 cgaatagggt cgaagaagcg gccgacgtag agggtgaacg aggtgaggac gccgaagaag
     2041 acggcgaggt cggcgtcggt cgagttcagg acgcggaggc cgccgacgat gaggacggcg
     2101 gcggtcgcga tggcgacggc gagctcgagg gcgggcatga cagccgcggt catgacggcg
     2161 gcgcggatgt tggcggcgcg gttctggcgg ttcagctgat cgaagcggcg gctgttctcg
     2221 tcctcgcggg acatcgactg gacggcgcgg acgccggaga ggttctcggc cagggtgccg
     2281 tagagctggc tgacctggac gcggacgttg acgaaggcgt ccttggcgtg gcgggaccac
     2341 cagaccatga cgagcacgag cgggggcacg acggcgaagg tgacgagcgc gagctgccag
     2401 tcccggacca gcagcacgat gacgatgatg ccgaggccga cgatgtcggc ggcgaaggtc
     2461 agcgagccgc tcgtcaggag gtcctggatg acggtgacgt cactggtgag ccggctgatc
     2521 atgcggccga cttccatctc gtcgtagaag ctgagcgaga ggctctgcat gtggtcgtac
     2581 atgtccgtac ggagcttcac gagcaggcgg ttgccgagcc agttgaccgc gagctgctgg
     2641 gcgaaggagg cgagccagta gaggaccgcc atcaccgcga ggacgatcag gaggatcacg
     2701 acctgccgtt cgtcgcggcg gacgccggcg gtgacggcga agccgatgat cagtggctgc
     2761 gcgaagttca tggcggctga gagcaccatg gcgaggaagg cgatcgtggc ctgcaatcga
     2821 tagggtcgca ggtaggggag gatgcggcga acgacctcgg cgtcgtaggc cttgccgaga
     2881 tactcgtcgt cccagccgtc gctgccgcga ccagcggcgc ggccgggccc catcgactgg
     2941 ccgccgatgc ctgtactcca gcccgcggcg cctccgccgc cccacattcc catcagccgg
     3001 tgacctcggc cgggcgggcg ggcaggggtg tggctccgga gccgttctgg tgagcggctc
     3061 ggtactcacc cagggcctct tcctggtcct tcaactcgag gtcaaagatc gtgcggtaga
     3121 ggccctcctg cgcgaggagc tgggcgtggc tgccgcgctg cacgatgcgg ccacgatcga
     3181 ggaccacgat ctcatcggcg cgcatcacgg tgcggaggcg ctgggcgatc acgaaggtgg
     3241 tgcggccccg cataagctcc tgcagcgcct gcaagatgag gtactccgtc tgggcgtcca
     3301 cgctcgcggt ggagtcgtcg aagatgagga tgcgcggatc gaggagcaag gtgcgggcga
     3361 tggcgatgcg ctgcttctgg cctccggaca gcgtcacgcc tcgttcgccg acccattcgt
     3421 cgtagccgag ggggaggctg acgatgaagt cgtggatgcg cgcggccttc gcggcgcgct
     3481 cgatctcctc ctgggaggcg cccgggcggc cgtaggcgat gttgtcgcgg atggtgccga
     3541 tgaagaggaa gacgtcctgc tggacgatgc cgatgttgcg gcggagggac tcgatggtca
     3601 c
//
LOCUS       DAORPG010000130         3571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2268_length_3571_cov_2.946530, whole genome shotgun sequence.
ACCESSION   DAORPG010000130 DAORPG010000000
VERSION     DAORPG010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2268_length_3571_cov_2.946530"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..171
                     /locus_tag="PLX85_03975"
                     /pseudo
     CDS             1..171
                     /locus_tag="PLX85_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(211..462)
                     /locus_tag="PLX85_03980"
                     /pseudo
     CDS             complement(211..462)
                     /locus_tag="PLX85_03980"
                     /inference="COORDINATES: protein motif:HMM:NF037039.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            624..3302
                     /gene="acnA"
                     /locus_tag="PLX85_03985"
     CDS             624..3302
                     /gene="acnA"
                     /locus_tag="PLX85_03985"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HRC62365.1"
                     /translation="MAGFESMNSFGTRATLTVGGQDHTIYRLDAAAQALGLDLARLPF
                     TLRILLENLLRGEDGSNVTADHIRAFAQWDPRAEPTDEIAFRPSRVLLQDFTGVPCIV
                     DLAAMRDAMVALGGDPKRINPLQPVDLVIDHSVQVDEFGNRLAFMHNVEREYERNRER
                     YQFLRWGQQAFQNFRVVPPGTGIVHQVNLEYLAQVVFRGDGAAYPDSLVGTDSHTTMV
                     NGLGVLGWGVGGIEAEAAMLGQPVAMLLPQVVGVRLTGRLPEGSTGTDLVLRVTELLR
                     GRNVVGKFVEFFGSGLGSLPLAARATIANMCPEYGATVGFFPVDEETLTYLRFTGRDE
                     IVPLVESYCREQHLFRTDATSDPEYSEVLELDLGSIEPSLAGPKRPQDRIALKDAKAS
                     AEQTIADEVKAGASAEPVHVGENGVAYDLTNGSVVIAAITSCTNTSNPEVMVGAGLLA
                     KKAVERGLARKPWVKTSLAPGSTVVTQYLDEAGLTPFLDQLGFNLVGYGCTTCIGNSG
                     PLPEPISNAVTEGKLAVAAVLSGNRNFEARISNQVRANYLASPPLVVAYALAGHMGVD
                     LMTEPLGTDTAGQPVYLRDIWPTTAEVQEVIQSSIRSQMFRAQYADVFTGDERWRGMD
                     VAPGELFQWDPDSTYVRRPPYFEDLAREPGEVRNIEGARVLALLGDSVTTDHISPAGD
                     IARTSPAAAYLASHGVEPRDFNSYGSRRGNHEVMMRGTFANVRLKNALVEREGNWTKH
                     FPDGEEGSIWDTAQRYQDEGVPLVVLAGREYGSGSSRDWAAKGPMLQGVRAVIAQSFE
                     RIHRSNLIGMGILPLQFPDGESAASLGLTGAEVFHIEGIEGGARPGQDVPVRAVAADG
                     AETRFTARCRIDTPVEADYYRHGGILQYVLRDLLAR"
     gene            3313..>3571
                     /locus_tag="PLX85_03990"
     CDS             3313..>3571
                     /locus_tag="PLX85_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRC62366.1"
                     /translation="MTTEQDVDTSSWKQTPDGIPIPPPSPEDVLYENRDGVGWITLNR
                     PRVLNAVNKNVTRLLAAALDQAAADEDAKAVILIGAGRAFCA"
BASE COUNT          569 a         1349 c         1140 g          513 t
ORIGIN      
        1 atgaagaccg tgcaaggtac gcacgggctg ctgtccaaca ccgaatggac aggcgttcgc
       61 ttcgccacga tcgcgcgcga gatcggcctg aagccgggtg cgcagtgggt gctggcggaa
      121 ggcgccgaca gcacggtcat gacccgttcg atcccgctcg agaagctcgt cgcggccgac
      181 ccggagcagc cagcgcagcg gtcgaggcag tcccgggaag tcgtcgccga aatactcggc
      241 tgcgagccgc ttcgcctcgt gcccgtggcg gtcatggtcg agcgaggcga ggacatccat
      301 gtaccgcagc agccgcgtgc ggaaccgctc gtcggccacc gcgttcgtga gcagccagtc
      361 ctcgatgcgc tccgccggcc ccgcgcggta ccccgcggcc cgttcgagca gggcgcggcc
      421 gagttcctgt gtccgagctt cgagggcggg atcgagtgcc atgtgtcgag gatagccgct
      481 gcgccgtccc cacaccgggc gaggtttccc gtgcgccgga ttgggctagg atacgccgag
      541 tcgaggccgc cgcccccgac cggcttcctc gctacactga caacagacga cgattgcgga
      601 cacctgagag cgggagacgc cccatggcgg gtttcgagtc aatgaatagc ttcggcacgc
      661 gtgcgaccct caccgtcggc ggccaggatc acacgatcta ccgcctcgac gccgcggcgc
      721 aggcgctcgg cctcgacctc gcgcggctac ccttcacgct gcgcatcctc ctcgagaacc
      781 tccttcgagg tgaggacggc agcaacgtca cggcggacca catccgcgcc ttcgcgcagt
      841 gggacccccg ggccgagccg accgatgaaa tcgcgttccg cccctcgcgc gtcctgctcc
      901 aggacttcac cggcgtcccc tgcatcgtcg acctcgccgc catgcgcgat gcgatggtcg
      961 ccctcggagg cgacccgaag cgcatcaacc cgctccagcc cgtggacctc gtcatcgacc
     1021 actccgtcca ggtcgacgag ttcggcaacc gcctcgcctt catgcacaac gtcgaacgcg
     1081 agtacgagcg caaccgcgag cgctaccagt tcctgcgctg gggccagcag gcgttccaga
     1141 acttccgcgt cgttccgccc ggcacgggca tcgtccacca ggtgaacctc gagtacctcg
     1201 cacaggtcgt cttccgcggc gacggggcgg cgtaccccga ctcgctggtc ggtacggact
     1261 cacacacgac gatggtcaac ggcctgggcg tcctcggctg gggcgtcggc ggcatcgagg
     1321 cggaggcggc catgctcggc cagcccgtcg cgatgctcct cccgcaggtc gtcggcgtcc
     1381 gcctcaccgg ccgcctcccg gagggctcca cgggcaccga cctggtgctg cgggtcaccg
     1441 agctgctccg cggccgcaac gtcgtcggca agttcgtcga gttcttcggc agcggcctcg
     1501 gctcgctgcc cctcgccgcc cgcgccacga tcgcgaacat gtgcccggag tatggcgcga
     1561 ccgtcggctt cttcccggtc gacgaggaga cgctcaccta cctccgcttc actggtcgcg
     1621 acgagatcgt ccccctggtc gagtcctact gccgcgagca gcacctcttc cgcaccgacg
     1681 ccacctccga ccccgagtac tccgaggtgc tcgagttgga cctcggctcg atcgagccga
     1741 gcctggccgg cccgaagcgc ccgcaggacc gcatcgccct caaggacgcc aaggcctcgg
     1801 ccgagcagac catcgccgac gaggtaaagg cgggcgcctc cgccgagccc gtccacgtcg
     1861 gcgagaatgg cgtcgcctac gacctgacga acggctcggt cgtgatcgct gccattacct
     1921 cctgcacgaa cacctcgaac cccgaggtga tggtcggcgc gggcctcctc gcgaaaaagg
     1981 ccgtcgagcg cggcctcgcg aggaagccgt gggtgaagac gagcctcgcc cccgggtcca
     2041 cggtcgtcac gcaatacctc gatgaggcgg gcctcacccc gttcctcgac cagcttggct
     2101 tcaacctcgt cggctacggc tgcacgacct gcatcggcaa ctccggcccg ctgcccgagc
     2161 cgatctcgaa cgcagtcacc gagggcaagc tcgctgtcgc agccgtcctc tccggcaacc
     2221 gcaacttcga ggcgcgcatc agcaaccagg tgcgcgcgaa ctacctcgca tcgccgccgc
     2281 tggtcgtcgc ctacgccctc gcgggccaca tgggcgtcga cctcatgacg gagccgctcg
     2341 gcaccgatac cgccggccag ccggtgtacc tccgcgacat ctggcccacc actgccgagg
     2401 tgcaggaggt cattcagtcc tcgatccgct cgcagatgtt ccgtgcgcag tacgcggacg
     2461 tcttcacggg cgacgagcgc tggcgaggca tggacgtcgc ccccggcgag ctgttccagt
     2521 gggaccccga ctccacctac gtccgccgcc cgccgtactt cgaggacctc gcgcgcgagc
     2581 ccggcgaagt gcgcaacatc gagggcgccc gggtcctcgc gctcctcggt gactccgtca
     2641 cgaccgacca catctcaccc gccggcgaca tcgctcgcac gagcccggcc gccgcgtacc
     2701 tcgcgagcca cggcgtcgag ccgcgcgact tcaactcgta cggatcgagg cgcggcaacc
     2761 acgaggtgat gatgcgcggc acgttcgcca acgtgcgcct gaagaacgcg ctcgtcgagc
     2821 gcgagggcaa ctggacgaag cacttccccg acggcgagga aggctcgatc tgggacaccg
     2881 cccagcgcta ccaggacgaa ggcgtcccgc tggtcgtcct cgctggccgc gaatacggct
     2941 ccggctcctc gcgagactgg gctgcgaagg gcccgatgct gcagggcgtc cgcgccgtca
     3001 tcgcgcagtc cttcgagcgc atccaccgca gcaacctcat cggcatgggc atcctgccgc
     3061 tccagttccc cgacggcgag tcggccgcct cgctcggcct gaccggcgcc gaggtcttcc
     3121 acatcgaggg gatcgagggc ggcgcacgcc ccggccagga tgtcccggtg cgtgccgtcg
     3181 ccgccgatgg cgcggagaca cgcttcacgg cccgctgccg catcgacaca ccggtcgagg
     3241 cggactacta ccgccacggc ggcatcctcc agtacgtgct gcgcgacctg ctcgcgcgtt
     3301 aggagcctca tcatgacgac cgagcaagac gtcgacacga gcagctggaa gcagacgcca
     3361 gacggcatcc cgatcccgcc gccctccccc gaggacgtgc tctacgaaaa ccgggacggt
     3421 gtcggctgga tcacgctgaa ccgtccgcgt gtcctgaacg ccgtcaacaa gaacgtcacg
     3481 aggctcctcg cggccgccct ggaccaggcc gcggccgacg aggacgcaaa ggcggtcatc
     3541 ctcatcggcg ccgggcgcgc cttctgcgcc g
//
LOCUS       DAORPG010000131         3567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2271_length_3567_cov_3.009966, whole genome shotgun sequence.
ACCESSION   DAORPG010000131 DAORPG010000000
VERSION     DAORPG010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2271_length_3567_cov_3.009966"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..117)
                     /locus_tag="PLX85_03995"
                     /pseudo
     CDS             complement(1..117)
                     /locus_tag="PLX85_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166474.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
     gene            149..1339
                     /locus_tag="PLX85_04000"
     CDS             149..1339
                     /locus_tag="PLX85_04000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC62367.1"
                     /translation="MASLTRDLARDFAKNEVAPRAQEIHRKDLLVPEDLIQKFAAQGF
                     FGSSIPEEYGGTGMGDLSMIIITEELSAASLAAAGSLATRPEILSKALLAGGTDEQKR
                     EWLPRLATGQTIVAVSVTEPDIGSDNAGVQTRAEPAEYNGQRGWRINGAKAWCTFSGR
                     ADVLSMLVRTDPDTSKGARGLSLLIVPKAPYYGHEWKHEQAEGGTISGTANPTPGYRG
                     MHSFTLAIDNYWVSHDNLVGGDAGIGQGFFLQMGGFAAGRLQTGGRATGVSQAALEKA
                     CQYVAQRQQFGLPLAEYQLTQYRIGRMSALISAGRQLTYAAARNFDSRGVEAPMAKLL
                     ACDVAVEVTQSAQQLHGGWGYSEEDPIARYVVDALVLPIFEGVKPILELKVIGRQLLA
                     QAAK"
     gene            1360..1770
                     /locus_tag="PLX85_04005"
     CDS             1360..1770
                     /locus_tag="PLX85_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018348354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRC62368.1"
                     /translation="MSEDAAAPKQRCLLITSDEAGESHFGDLELPLAGDSFAPPAPPL
                     FTSGRVAAERVLFFHAPVGWFGDWHPTPARQFFIALSGDLEVHVSDGEVRHVRGGDIV
                     LLEDLSGRGHTTRVVGDEPACAAVVQLGDAELPL"
     gene            complement(1767..2129)
                     /locus_tag="PLX85_04010"
     CDS             complement(1767..2129)
                     /locus_tag="PLX85_04010"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HRC62369.1"
                     /translation="MRSELLLDEMYSPSIAVQLRQRGHDVESVVERTDLRTASDEAVF
                     AVGRRETRVIVTENIGDYQREARIAVEGGAAHPGLIYTTNARFPRGDARTVGRPVLAL
                     DDLLRGGIDLTDREYWLR"
     gene            complement(2119..2517)
                     /locus_tag="PLX85_04015"
     CDS             complement(2119..2517)
                     /locus_tag="PLX85_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62370.1"
                     /translation="MPRATLQRLDEESKREHVSRSELARTFIEEALRMEAHRGIVFKP
                     GPAGRRPALAAGPDVWEIVRAWHDLGVPEPEAIPRLAEIASVPAFEIERALRYYADYP
                     HEIDAWIGRMNADTERAFEEWLRQQATAEE"
     gene            complement(2583..2942)
                     /locus_tag="PLX85_04020"
     CDS             complement(2583..2942)
                     /locus_tag="PLX85_04020"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRC62371.1"
                     /translation="MRVIKASEGTSQERTGQPIFEGQVWGRGLVEGEGHLTASIVQFA
                     VGGRTRMHTHTSDQLLYVVAGIGKVGTAEGEQLISTGDVALIPAGEAHWHGAGDSGSP
                     MSHLTVMRADSETEVLG"
     gene            3182..>3567
                     /locus_tag="PLX85_04025"
     CDS             3182..>3567
                     /locus_tag="PLX85_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62372.1"
                     /translation="MQPTHQDDSQPYHLVVGDVTGSDRAPSFRDPALGRVQVRIDGEL
                     AGHPHAHELVSGLAEQLAGHAAAGGTALHFEVDDEALRGGASVREAARLASDPFRRAE
                     AVAVEHLQSWLSARGPVARPTGEAKA"
BASE COUNT          527 a         1175 c         1296 g          569 t
ORIGIN      
        1 gaagaacgtc atgacgccgt cttccagcga ggtcgccggc cgcgcatagc cggcggcgcg
       61 cagcttctcg atgcgcccct cggtgaagta ctggtagcgc gcgcggatcg cctcgggggg
      121 cgtcaacaac atcgagctgc aggacgaggt ggcgagcctg acgcgcgacc tcgcgcgcga
      181 cttcgcgaag aacgaggtcg cgccgcgcgc gcaggagatc catcgcaagg acctcctggt
      241 gccggaggac ctgatccaga agttcgctgc acagggcttc ttcggctcct cgatcccgga
      301 ggagtacggc gggaccggca tgggcgacct ctcgatgatc atcatcacgg aggagctctc
      361 ggccgcctcg ctggccgcgg ccgggtcgct ggcgacgcgg ccggagatcc tgtcgaaggc
      421 gctgctcgcg ggcgggacgg acgagcagaa gcgggagtgg ctgccgcgcc tcgcgacggg
      481 gcagacgatc gtcgcggtgt cggtgaccga gccggacatc gggtcggaca acgcgggcgt
      541 ccagacgcgc gctgagccgg ccgagtacaa cggccagcgc ggctggcgga tcaacggcgc
      601 caaggcgtgg tgcacgttct cagggcgcgc ggacgtgctc tcgatgctgg tgaggacgga
      661 cccggacacc tcgaagggcg cccgcgggct gtcgttgctg atcgtgccga aggcgccgta
      721 ctacggccac gagtggaagc acgagcaggc ggagggcggg acgatcagcg ggaccgcgaa
      781 cccgacgccc gggtatcggg ggatgcactc gttcacgctc gccatcgaca actactgggt
      841 gtcgcacgac aacctcgtgg gtggggacgc gggcatcggc cagggcttct tcctgcagat
      901 gggcggcttc gcggcggggc gcctgcagac cgggggccga gcgaccggcg tgtcgcaggc
      961 ggcgctcgag aaggcgtgcc agtacgtggc gcagcggcag cagttcgggt tgccgctcgc
     1021 ggagtaccag ctgacccagt accgcatcgg gcggatgtcg gcgctcatca gcgccgggcg
     1081 gcagctgacg tatgcggcgg cgcgcaactt cgacagccgg ggggtggagg cgccgatggc
     1141 caagctcctc gcgtgcgacg tggccgtcga ggtgacacag tcggcgcagc agctgcacgg
     1201 tggctggggc tactcggaag aggacccgat cgcgcggtat gtcgtggacg cgctggtgct
     1261 gccgatcttc gagggcgtga agcccatcct cgagctgaag gtgatcggcc ggcagctgct
     1321 ggcgcaggcg gcgaagtagt cgggcgagca ggcgcggcga tgtcggaaga tgccgccgcg
     1381 ccgaagcaac gctgcctgct gatcacctcg gacgaggcgg gggagtcgca cttcggcgac
     1441 ctcgagttgc cgcttgcggg cgactcgttc gcgccgccgg cgcctcctct cttcacctcg
     1501 ggacgggtcg cggcggagcg ggtgctgttc ttccacgcgc cggtaggctg gttcggggac
     1561 tggcacccga ctcccgcgcg tcagttcttc atcgcgctca gtggtgacct cgaggtgcac
     1621 gtgagcgatg gcgaagtcag acacgtgcgc ggcggcgaca tcgtgctgct cgaggatctt
     1681 tcggggcggg gccacacgac ccgggtggtc ggcgacgagc ccgcgtgcgc cgcggtcgtg
     1741 cagctgggtg acgcagagtt gcctctctaa cgcagccagt actcgcggtc cgtgaggtcg
     1801 atcccgccgc ggagcaagtc atccagagcg agcaccgggc ggccgaccgt cctcgcatct
     1861 ccgcggggga agcgggcatt ggtcgtgtaa atcaggccgg ggtgcgcggc accgccctcg
     1921 acggcgatcc tggcctctcg ctggtagtcg ccaatgttct ccgtgactat tacgcgcgtc
     1981 tcacgccttc cgacggcaaa gacggcctcg tcggaggctg tccgcaagtc ggtgcgctcc
     2041 acgacgctct cgacatcgtg tcctcgctgc cgcagttgaa ccgcaatgga cggcgagtac
     2101 atttcgtcga ggaggagttc actcctcagc ggtggcctgc tgccgcaacc actcttcgaa
     2161 cgctcgttca gtgtcagcgt tcatccgacc gatccacgcg tctatctcat ggggatagtc
     2221 ggcgtagtag cgcagggcac gctcgatctc gaacgccggg actgaggcga tttccgccag
     2281 ccgcgggatt gcctcgggct ctggcactcc cagatcgtgc caggcacgga cgatctccca
     2341 gacgtctggc cccgcggcga gcgctggccg gcggcccgcc ggccctggct tgaatacgat
     2401 gccgcgatgt gcctccatac gcagcgcctc ttcgatgaag gtccgtgcca gctcggagcg
     2461 cgacacgtgc tcacgcttgc tctcttcgtc gaggcgctgg agggtggccc tggggattcg
     2521 gatcgagacc tgatcggtgg tcgtgattcc tccgtcacca gcgtatcagc gcgtgggcgc
     2581 cctcagccga gcacctcggt ctcgctgtcg gcgcgcatga cggtgaggtg tgacatcggc
     2641 gagccgctgt cgccagcacc gtgccagtgg gcctcgccgg ccgggatcag ggctacgtcg
     2701 cccgtcgaga tgagttgctc gccctcggcg gtgccgacct ttccgatgcc ggcgaccacg
     2761 tagagcagct ggtcactcgt gtgggtgtgc atccgcgtgc gtccgccgac cgcgaactgc
     2821 acgatcgagg cggtcaggtg cccctcgccc tcgacgagcc cgcggcccca cacctgtccc
     2881 tcgaagatgg gctgccccgt gcgttcctgg gatgtgccct cagacgcttt gatcactcgc
     2941 atcggttcct cctcacgctg actgtcaggg ttgctcgaga gtcccctctc gaagcgggcc
     3001 gggagacttg tcgagcgtat ccgatcgccg tctcgatgcg ccgtcgggca atcgatcctc
     3061 gctccggtga ttcccgcgtg gtccccgccg cgggcgtgcc gtactacatc cggcgcgaat
     3121 gcgcggctat agtgcacgcc acccgagggc cgctggctcg aggtcgcgga gaggcgcgga
     3181 catgcaacca acccaccagg acgacagtca gccgtaccac ctcgtggtcg gcgatgtgac
     3241 cggcagtgac cgggcgccca gctttcgcga tcccgcgctc ggcagggtgc aggtacggat
     3301 cgatggagag cttgccggtc acccacacgc gcacgagctg gtatccggtc tggcggagca
     3361 gctcgcgggg cacgcggcgg ccggcggcac ggcgctgcac ttcgaagtcg acgacgaggc
     3421 gttacggggt ggcgcgagcg tccgggaggc ggcgcgactg gcctctgacc cgttccgccg
     3481 ggccgaggca gtggccgtcg agcaccttca gagctggctg tcggcgcgcg gccctgttgc
     3541 gcgcccgacg ggtgaggcga aggccga
//
LOCUS       DAORPG010000132         3561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2281_length_3561_cov_3.258985, whole genome shotgun sequence.
ACCESSION   DAORPG010000132 DAORPG010000000
VERSION     DAORPG010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2281_length_3561_cov_3.258985"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..926)
                     /locus_tag="PLX85_04030"
     CDS             complement(132..926)
                     /locus_tag="PLX85_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC62373.1"
                     /translation="MADKPLEGKVAIVTGSSRGIGEAIARSFARNGATVVVAARTEEV
                     SDARLPGTIHSVAAAIEAEGGKAFPLVTNLRDPESIYACVDTTVKEFGRLDILVNNAA
                     ILVPGDIETVQDRHLDLMWQVDLRGPVLMCKAAVPHLRATGGGHIINISSGVAIFPGP
                     GPYENPSTGGLFYGMVKAGLERFTQSLAQDLQKDRIAANVLSLAYVIETPGNIFARND
                     PNNPRLDFGSAEWMGKAATWIAKQPAEYTGNIVFDHKIRYWLEDIP"
     gene            complement(1168..1539)
                     /locus_tag="PLX85_04035"
     CDS             complement(1168..1539)
                     /locus_tag="PLX85_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRC62374.1"
                     /translation="MTADRRERATEVRRAVLGTSVPANPPADDTAAAFSDAALDFVWG
                     TIWARDVISRRDRSIATLSMLTALNRPHELRIHVQVALNNGLMREEILEILLHASVYC
                     GFPAAVDAMRIAREVFAETAS"
     gene            complement(1616..2029)
                     /locus_tag="PLX85_04040"
     CDS             complement(1616..2029)
                     /locus_tag="PLX85_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HRC62375.1"
                     /translation="MTTAIPDKPLPFRTSWTQSFWDGTKRGEFLIQRCSNCDTPRFPP
                     KPVCSNCWSTDYREVPAAGTGTVYTFTVQYRAGVPAFANDVPYAIVLVELDEGVRVMS
                     DMVNCDAEEVHIGMPVKLVWDPPSEDLTMYKFEPA"
     gene            complement(2026..3219)
                     /locus_tag="PLX85_04045"
     CDS             complement(2026..3219)
                     /locus_tag="PLX85_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62376.1"
                     /translation="MAITASGTPAVVGTYVTEQGPLEGRTTSSIYVESVLGALADAGL
                     SPGDVDGLVGAGPGNPALWNPHGVYVDLFGTPLRFYAEAKVGAGSPGAAIALAASAIR
                     EGRAEVVLVANAAGAPDMAHAGGLGADRDSAVARMSMLGSEFEYPWGTTRIADYAMYA
                     QRHMYEYGTKPEQLAEVAVAARYHATLTPEAVMGPRGPITIDDVLASRMIASPLHLLD
                     CCLINNGGGAYVVTSEARARSLRHDPVFLLGFGENYPFNDRHYARSLSGFDTHNSAGQ
                     ALSQANLTHDDIDLAGVSDHFTINVLIELEDAGFCKKGEGGSFVEGGALQIGGRLPTN
                     TDGGYLSHSHAGGCGVFTVIEAVTQLRREAGARQVQDATFAMLRGTGGAMQASYTAVL
                     GRDIP"
BASE COUNT          525 a          999 c         1434 g          603 t
ORIGIN      
        1 cgacgtacgg tgggtcgctg atgatctact cgtccgacgg gatcatcgtg gcgttctggt
       61 tcaacgacgc gaacctgatc gaccgcgtga ggctcgcgaa cgggcggctc ctcgggccgt
      121 aggcgcctcg atcaggggat gtcttcgagc cagtagcgga tcttgtggtc gaagacgatg
      181 ttgcccgtgt actcggcggg ctgcttcgcg atccaggtgg cggctttacc catccactcg
      241 gcgctgccga agtcgaggcg ggggttgttg gggtcgttgc gggcgaagat gttgcccggc
      301 gtttcgatga cgtaggcgag cgagagcacg ttggcagcga tgcggtcctt ctggagatcc
      361 tgcgcgaggc tctgtgtgaa gcgctcgagg ccggctttga ccatgccgta gaaaagaccg
      421 ccggtgctcg ggttctcgta gggacccggg cccgggaaga ttgcgacgcc cgaggagatg
      481 ttgatgatgt ggccgccacc tgtggcgcgg aggtgaggga cggcggcctt gcacatgagg
      541 acggggcctc ggaggtcgac ctgccacatg aggtcgaggt ggcggtcctg gacggtctcg
      601 atgtcgcccg ggacgagaat ggcggcgttg ttgacgagga tgtcgaggcg accgaattcc
      661 ttcacggtgg tgtcgacgca agcgtagatg ctctcggggt cgcggaggtt agtgacgagg
      721 gggaaggcct tgccgccctc ggcctcgatg gcggcggcca cggagtggat ggtgccgggg
      781 aggcgggcgt cggagacctc ctcggtacgg gcggcgacga cgacggtggc gccgttgcgg
      841 gcgaaggagc gggcgatggc ttcgccgatg ccgcggctgg agccggtcac gatcgcgact
      901 ttgccttcga gcggtttgtc ggccacgggt gatcctcctt cgtcgagtgc gtcctcggag
      961 ggatgcatat cacggacaag gggtgcggcg ccacggcgca ggggttggat cgcggccggc
     1021 tgttgttttc ggagagttcc gcgcctctgt gctggaaagg ggctccgccc ctcgatcccc
     1081 tgctgaggca acgtgtgtcg gcaggagcgg tccgtgcctg gtatgcccga cgaggcgggt
     1141 gattcggcag agcggcttgg tgtggggtta gctcgcggtt tcggcgaaga cctcgcgggc
     1201 gatgcgcatg gcgtcgacgg cggcggggaa gccgcagtag acggaggcgt ggaggaggat
     1261 ctcgaggatt tcttcccgca tgaggccgtt gttgagggcg acctggacgt ggatgcggag
     1321 ttcgtgcggg cggttgaggg ccgtgagcat cgagagggta gcgatgctgc ggtcgcgccg
     1381 cgagatcacg tcgcgggccc agatggtgcc ccagacgaag tcgagggcgg catccgagaa
     1441 ggcggcagcc gtgtcgtcag cgggtgggtt ggcggggacg ctcgtgccga ggacggcgcg
     1501 tcggacctcg gtggctcgtt ctcggcggtc ggcggtcatg cgcgtctcct cgaaatcgcg
     1561 gcctccgtgc tggtgctcga gctaaccctc gagcaccagc acgcgggcgg gcgggtcagg
     1621 cgggctcgaa cttgtacatc gtgaggtctt ccgaaggtgg atcccagacg agcttcacgg
     1681 gcataccgat gtgcacctct tcggcgtcgc agttgaccat gtcggacatg acgcggacgc
     1741 cctcgtccag ctcgacgagg acgatggcgt aggggacgtc gttggcgaag gcggggacgc
     1801 cggcgcggta ctggaccgtg aaggtgtaga cagtgccggt gccagccgcc ggcacctccc
     1861 ggtagtcggt ggaccagcag ttggagcaga ccggcttcgg cgggaagcgc ggggtgtcgc
     1921 agtttgagca gcgctggatg aggaactcgc ctcgcttcgt gccgtcccag aaggactgcg
     1981 tccacgaggt gcggaagggg agtggcttgt cggggatcgc ggtggtcacg ggatgtccct
     2041 tcccaggact gcggtgtagg aagcctgcat ggcgccgccc gtgccgcgaa gcatggcgaa
     2101 ggtggcgtcc tgcacctggc gcgcgccggc ctcgcggcgg agctgggtga cggcctcgat
     2161 gacggtgaag acaccgcagc ccccggcgtg gctgtgcgag aggtagccac catcggtgtt
     2221 ggtggggagg cgtccgccga tttgcagcgc gcccccttcg acgaacgagc cgccctcgcc
     2281 cttcttgcag aagccggcat cctcgagctc gatgaggacg ttgatcgtga agtggtcact
     2341 gacgccggcg aggtcgatgt cgtcgtgcgt gaggttggcc tggctgagcg cctggccagc
     2401 ggagttgtgc gtgtcaaagc cgctgaggct cctggcgtag tgacggtcgt tgaaggggta
     2461 gttttcgccg aagccgagca ggaacaccgg gtcatgtcgc aggctgcggg cgcgagcctc
     2521 gctggtcacg acgtaggcac caccgccgtt gttgatcagg cagcagtcga ggaggtgcag
     2581 ggggctggcg atcatcctcg aggcgaggac gtcatcgatg gtgatggggc cgcgcggacc
     2641 catgacggct tccggggtga gggtcgcatg gtagcgagcg gcgacggcga cctcggcgag
     2701 ctgctcgggc ttcgtgccgt attcgtacat gtggcgctgg gcgtacatgg cgtagtcagc
     2761 gatgcgggtg gtgccccagg ggtactcgaa ttcgctgccc agcatggaca tcctcgcgac
     2821 ggcggagtcg cggtcggcgc cgaggccgcc ggcatgggcc atgtcgggcg cgccagcggc
     2881 gttggcgacg agtacgacct cggcacggcc ttcgcggatg gcggatgcag cgagggcgat
     2941 ggcggcaccg ggggagccgg caccgacttt ggcctcggcg tagaagcgca gaggcgtgcc
     3001 gaagaggtcg acgtagacgc cgtgggggtt ccagagcgcg gggttgccgg gaccggcgcc
     3061 cacgaggcca tcgacgtcgc cgggggagag cccggcatcg gcgagggcgc cgaggaccga
     3121 ctcgacataa atactcgagg tagtgcggcc ctcgaggggt ccctgttcgg tgacgtaggt
     3181 gccgacgacc gcgggcgtgc ctgacgctgt gatcgccatc gctccctcct ttcattggag
     3241 gcggaccgaa tgtcctcgga gcgtggggcg ggcgtcctcg gagcgggcgt catcacgtcc
     3301 gagtgtgtgg atggcggcat tgtagtaccg ggggtcgcgg gcgtgtgggc ggcgcgccgc
     3361 cgttcaacac gccgagggat ttcggatatg atgcgcgcgc ccgtgggagc cggggcagcg
     3421 ctcccacgag gaaggggcgt gagatggtcg agaagccgac ggatacgggc gggtcgcagg
     3481 cgggtcagac gcagggcagc ggcggcctcg gctacgcggg gccgggcggc gagggcggag
     3541 cgctcgccgg gacgagcggg g
//
LOCUS       DAORPG010000133         3542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2304_length_3542_cov_3.887869, whole genome shotgun sequence.
ACCESSION   DAORPG010000133 DAORPG010000000
VERSION     DAORPG010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2304_length_3542_cov_3.887869"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /gene="glmU"
                     /locus_tag="PLX85_04050"
     CDS             <1..1171
                     /gene="glmU"
                     /locus_tag="PLX85_04050"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HRC62377.1"
                     /translation="AVRAQVPGAAVAPQGEPLGTGHAALAARVAAGDASHLLILNADL
                     PLITERTVRELTQRHLQEHAVLTFLTAYLEDPTGYGRVRRQNGRIHGIVEESDADAVT
                     RGQPEVNAGLYAAEAEWLWPVLAGLEPSPRGEVYLTDAIARAVDHPQGAEAYQVVESS
                     EVQQVNTRVELARAEQIMRERIRRRVMLDGVTLVDPATTYIDAGVAIGPDTTLLPGVH
                     LLGSTTVGGGCRIGPSAVVRDSTIGDGCEVGMSTLEGSELAERVTVGPYCHLRPGTRI
                     EADVHLGNYVEVKAARVGARTRVGHFSYIGDADVGADVNIGAGTVTANYDGATKHHTT
                     IGDRAFIGSDSVLIAPVELGADARTGAGTIVTRDVPASARVVGVPGRIREPGEGV"
     gene            1196..2479
                     /locus_tag="PLX85_04055"
     CDS             1196..2479
                     /locus_tag="PLX85_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRC62378.1"
                     /translation="MDTGRLLALVLLVVSSLILVLVSAAEASVSAITRSRARHGQPGH
                     DGSLNTLLDNYIRQRQRVLRSLSVAVTVSTVITALAITVLIVDGDEFTGWRMVIAALA
                     SVLVVGFLRQTARTFALINPENTGLRLARPILAVQAVCSPLAWAGSTPAAAIARAVGR
                     DPDVPEANPADELLSVLERTDNASGRALSEERRMMRGILAMSEQTVREVMTPRPDIQA
                     LAHDAAAGDVMRIINETGFSRVPIYEESMDHIVGVVYAKDLLAYLRAGRLNQTLREIA
                     RPPYFVPETKHVDELLADMRRDKVHMAVAVDEYGGTAGIVTVEDLLEEIVGEIADEYD
                     VEDVDVQQLSEDEAIVDARLSIDDLNELFGTEIDSEDFDTVGGLILSLLGRLAVPGDE
                     VTSEEYELQLRVLSILGRRIKKVRVTRLATESAPA"
     gene            complement(2480..>3542)
                     /locus_tag="PLX85_04060"
     CDS             complement(2480..>3542)
                     /locus_tag="PLX85_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009574377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRC62379.1"
                     /translation="GRAGKLLYRGYSIHDLAEHSTFEETAYLLFYSKLPTGDELSAFE
                     ADLKASRTLPDEIFDIIHAVRHAHPMDVLRTATSALAAFDPEVNDNSIEATVRKGVRL
                     TAQVPTIVMAHEAIRNGRQPVAPNLSLSHAANFLYMLTGEVPTDDAARLMDKDFILHA
                     EHGPNASAFTARVVAGTQANLHAAIVAAIGALSGPSHGGAAENVMQMAMEIGEPENAA
                     AYVQRLRSEGKPVMGFGHRVYKAEDPRARHLREGVRRLSLEREEPKWYHILEAVQEAM
                     RPYARLGVHVNVDFFAGVIYYLVGFPPDLFVPIFAVGRIPGWTAQVVEQFENNILIRP
                     LTQYVGEMDLPYIPIEERG"
BASE COUNT          564 a         1085 c         1340 g          553 t
ORIGIN      
        1 ggcagtacgg gcgcaagtgc cgggggcggc ggtcgcgccc cagggcgagc cgctcggaac
       61 agggcacgcg gcactcgccg cgcgggtcgc ggccggcgac gcctcacacc tgctcattct
      121 gaatgcggac ctgcccctga tcactgaacg cacggtgcgc gagttgacgc agcgtcacct
      181 gcaggagcac gcggtgctca ccttcctcac cgcctacctc gaggatccga ccgggtacgg
      241 gcgtgtgcga cggcagaacg gacgtatcca cggcatcgtg gaggagtcgg acgcagatgc
      301 cgtcacccgg gggcaaccgg aggtcaacgc cggactgtac gcggcggagg ccgaatggct
      361 gtggccggtg ctcgccggcc tcgagccgag cccgcgcggc gaggtgtacc tgaccgacgc
      421 gatcgcgcgg gcggtcgacc acccgcaggg cgccgaggca tatcaggtcg tggagtcctc
      481 ggaagtgcag caggtcaaca cgcgtgtcga gctggcccgc gcggagcaaa tcatgcgcga
      541 gcgcatccgc cggcgcgtca tgctcgacgg ggtgacgctc gtcgacccgg ccacgaccta
      601 cattgacgcg ggcgtcgcga tcgggccgga cactaccctg ctccccggtg tccacctcct
      661 cggcagcacg accgtgggcg gcgggtgccg catcgggcct tccgcagtgg tgcgcgattc
      721 gacgatcggc gacggctgcg aggtcgggat gtcgaccctc gaagggtcgg agctcgcaga
      781 gcgagtcacg gtggggccgt actgccatct gcgaccgggg acacggattg aagcggacgt
      841 ccaccttggg aattatgtcg aggtaaaggc ggcgcgcgtg ggagcgcgca cacgggtggg
      901 ccatttctcg tacatcggcg acgcggacgt tggggctgac gtgaacatcg gcgccgggac
      961 cgtgacggcg aactacgacg gcgccacgaa gcaccacacg acgattggcg accgcgcctt
     1021 catcgggagc gattcggtgt tgatcgcgcc agtcgagctg ggcgccgacg cgcggaccgg
     1081 tgcggggacg atcgtcacgc gcgatgtccc ggcgtcggcg cgtgtcgtgg gggtcccggg
     1141 acggatccgc gaacccggag agggggtctg aggtccccac ggggaccgat ctcgcttgga
     1201 taccggtcga ctcctcgccc tggtgctgct cgtcgtgtcg tcgctcatcc tcgtgctggt
     1261 gtcggcggct gaggcgagcg tgagcgcgat cacgcgtagt cgtgcgcggc acggccagcc
     1321 ggggcacgac ggcagtctga acaccctgct cgacaactac attcgccagc ggcagcgagt
     1381 gttgcgctcg ctcagcgtgg cggtgaccgt cagcacggtg atcacggcgc tggcgatcac
     1441 cgtcctgatc gtcgacggcg acgagttcac gggctggcgc atggtgatcg ccgcactggc
     1501 ctcggtgcta gtggtggggt ttctgcggca gacggcgcgc accttcgcac tgatcaaccc
     1561 tgagaacacc gggctgcggc tggcgcggcc gatcctcgcc gtacaggcgg tgtgttcgcc
     1621 gctggcatgg gccgggagca cgcccgcggc ggcgatcgca cgtgcggtgg ggcgggaccc
     1681 ggacgtcccg gaggcgaacc ccgctgacga gctgctgtcc gtcctcgagc ggaccgacaa
     1741 cgcgtcggga cgtgcactgt cggaggagcg gcggatgatg cgtggcatcc tcgcgatgtc
     1801 ggagcagacc gtgcgcgagg tgatgacgcc acgcccggac atccaggcgc tggcgcacga
     1861 cgcggcggca ggcgacgtga tgcggatcat caacgagacc ggcttcagcc gcgtcccgat
     1921 ctacgaggag tcgatggacc acatcgtggg ggtggtctac gcgaaggacc tgctcgccta
     1981 cctgcgggcc gggaggctca accagactct ccgcgagatc gcgcggccgc cttacttcgt
     2041 gccggagacc aaacacgtcg acgagctgct cgcggacatg cgtcgggaca aggtgcatat
     2101 ggcggtggcc gtcgatgagt acggcggcac ggcgggcatc gtgacggtcg aggaccttct
     2161 cgaggagatc gtgggagaga tcgcggacga gtacgacgtc gaggatgtgg acgtgcagca
     2221 gctgtcggag gacgaggcga tcgtggacgc gcgcctttcg atcgacgacc tcaacgagct
     2281 attcggcacg gagatcgaca gcgaagactt cgacacggtc ggcggactga tcctgtctct
     2341 cctcggacgg ctggcggtgc cgggcgacga ggtgaccagc gaggagtacg agctccagct
     2401 gcgggtgctg tcgatcctcg ggcggcggat caagaaggta cgcgtgacac gactggcgac
     2461 ggagtcggca ccagcctgat cagccgcgct cctcgatcgg gatgtagggg agatccatct
     2521 cgcccacgta ctgggtgagc gggcggatga ggatgttgtt ctcgaactgc tccacgactt
     2581 gggcggtcca gccggggatg cggcccacgg cgaagatggg gacgaagagg tcgggcggga
     2641 agcccacgag gtagtagatc acgccggcga agaagtcgac gttcacgtgg acgccgaggc
     2701 gcgcgtacgg gcgcatcgcc tcctggaccg cttcgaggat gtggtaccac ttcggttctt
     2761 cgcgctcgag gctcaggcgt cgcacgccct cgcggagatg gcgtgctcgg gggtcctccg
     2821 ccttgtagac gcggtgaccg aagcccatca ctggcttgcc ctccgagcgg aggcgctgca
     2881 cgtaggcggc ggcgttttcg ggctcgccga tctccatcgc catctgcatg acgttctcgg
     2941 cggcgcctcc gtgcgagggg ccggagagtg caccgatggc ggcgacgatg gcggcgtgca
     3001 ggttcgcctg cgtgccggcg acaactcgcg ccgtgaacgc ggaggcgttg gggccgtgct
     3061 cggcgtggag gatgaagtct ttgtccatca ggcgcgcggc gtcgtctgtc ggaacctcgc
     3121 cggtcagcat gtacaggaag ttggcggcgt gggagaggct caagttcgga gcgaccggtt
     3181 ggcggccgtt cctgatcgcc tcgtgagcca tgacgatcgt cgggacctgc gcggtgagcc
     3241 ggacgccctt gcggacggtc gcctcgatgg agttgtcgtt cacctcgggg tcgaaggcgg
     3301 cgagtgctga ggtcgccgtg cgaaggacat ccatcgggtg ggcgtgccga acggcgtgga
     3361 tgatgtcgaa gatctcgtcg ggaagggtgc gggaggcctt gaggtcggcc tcgaaagccg
     3421 agagctcgtc gccggtgggg agcttgctgt agaagaggag gtaggccgtc tcctcgaacg
     3481 tggaatgctc ggcgaggtca tggatggagt agccgcggta gaggagcttg ccggcgcgac
     3541 cg
//
LOCUS       DAORPG010000134         3528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2322_length_3528_cov_3.323064, whole genome shotgun sequence.
ACCESSION   DAORPG010000134 DAORPG010000000
VERSION     DAORPG010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2322_length_3528_cov_3.323064"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PLX85_04065"
     CDS             complement(<1..564)
                     /locus_tag="PLX85_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62380.1"
                     /translation="MKTIDLVSIRKWLFLGSGLTALFGLILLAIPPTLKPGIEFTSGS
                     TMLIRFTNPVSQSDLRDEFTRLGHSEARIQSTGANEFLVRTKSLNVPAGSFNEVEPTA
                     TPTASATATATVGLGTVLIGKDGATGDIALRAVQGGDPCRLGIESGRVAANTQGTVLE
                     QHTNCASGTVYRVEAGGATGYIQASDTH"
     gene            complement(561..2015)
                     /gene="secD"
                     /locus_tag="PLX85_04070"
     CDS             complement(561..2015)
                     /gene="secD"
                     /locus_tag="PLX85_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRC62381.1"
                     /translation="MPTRAHLWPIAIIAVLSVAALIIVWPSQPERWFPSAIPWPAGKG
                     ISIGSFERKTMRLGLDLQGGTRLLLDASLPPGTEGDVGDAMDGTIRVLRKRVDSSGVS
                     EAEITRQGNSNISVQLPGLTPDKARDLLGRTALLQFCQQGQSPFPDVAQPCDDQGQWV
                     QAVAELDGKPVPLTSRFLKPNAFVGTDQLGNPVVDFEWNSDGAQLSKQVTQRLLGQPL
                     GIFLDNEALASPNVNGVIEDRGQITGLPLTTTKEGRTGAKDLVIQLNSGALPVQLTVL
                     QEQNVDATLGDDAVRQSVVAGEIGLLIVILFMVLYYRVPGILASVALIVYTLLSLAIF
                     KLIPVTMTLAGIGAFVLSVGMAVDANILVFERLKEEMREGRSYATAIEAGFARAWPSI
                     RDSNVTTLITTLILYVLGGGLTIPGLGAFEAPLVQGFALTLAAGVLVSMFSAIFVTRA
                     LMRLLIGTSISRQSDWLGADMRPIGEPSPAGGDG"
     gene            complement(2015..2335)
                     /gene="yajC"
                     /locus_tag="PLX85_04075"
     CDS             complement(2015..2335)
                     /gene="yajC"
                     /locus_tag="PLX85_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRC62382.1"
                     /translation="MGEFIFLALLIFLAFYFILLRPVIQQQQRRRRDISSLEIGDEVL
                     TTGGFFAVIRDIQTRDRGPLELTLEAAPGVLLRATADAVQSVSRHASEDLTEADDAGK
                     ASAN"
     gene            complement(2338..2877)
                     /gene="def"
                     /locus_tag="PLX85_04080"
     CDS             complement(2338..2877)
                     /gene="def"
                     /locus_tag="PLX85_04080"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRC62383.1"
                     /translation="MTVRPIRYLGDPVLRQPAKKVRQVDDSIRRLIVDMIESMYAAQG
                     VGLAAPQIGVPLRVVVLGMPDEEPFALINPVIVKRTGSRVLQGEGCLSVPGYRTHAIT
                     RSQKVVVRALDERGREVRMRAEDDLLAQAFEHEVDHINGFLFVDRLDSPSDLYKVDER
                     AVAARQAEPERTAAAGGDE"
     gene            2967..3359
                     /locus_tag="PLX85_04085"
     CDS             2967..3359
                     /locus_tag="PLX85_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62384.1"
                     /translation="MPRRLVRRSRSPARPGDWALVAVAKDEVTAEEWQRLLSGEGIEA
                     QVRIDDPARAGMPGRTAFPLGYTEPGLSLFSYPLYVRRQQRREARQLLGEQVEFSRSG
                     LSGQTVLGAVGVVVASLAIVILLVLRGS"
BASE COUNT          572 a         1202 c         1190 g          564 t
ORIGIN      
        1 gtgggtatcg gaggcctgga tgtagcccgt cgcgcctccg gcctccacgc ggtagacggt
       61 gccgctcgcg cagttcgtgt gctgctccag gaccgtcccc tgtgtgttgg cggccacgcg
      121 cccggactcg atgcccaggc ggcaggggtc gccgccttgc actgcacgca gcgcgatgtc
      181 gccggtggcg ccgtccttgc cgatcagcac cgtgccgagg ccgaccgttg cggtggcggt
      241 cgcggacgcc gtgggcgtcg ccgtcggctc cacctcgttg aaactgcccg cgggcacgtt
      301 gaggctcttc gtacgcacga ggaactcgtt cgcacctgtg gactggatgc gcgcctcgct
      361 gtgcccgagc ctggtgaatt cgtcacgcag gtcagactgg ctcaccgggt tggtgaaccg
      421 gatcaacatc gtgctgccgg aagtgaactc gatgcccggc ttcagcgtcg gcggaatggc
      481 gagcagaatc agcccgaaca gggccgtgag gccgctgccc aggaacaacc acttccggat
      541 cgataccagg tcgatcgtct tcatccgtcg ccccctgccg gggacggctc cccgatcggc
      601 cgcatgtcgg ctccaagcca gtcagactgg cgcgagatcg aagtcccgat gagcagacgc
      661 atcagcgccc gcgtcacgaa gatcgcggag aacatggaga cgagcacgcc ggccgccagt
      721 gtcagcgcga aaccctgcac gagcggcgcc tcgaacgcgc cgaggccagg aatagtgagc
      781 ccgccgccca ggacgtacag gatcaacgtg gtaatcagcg tcgtgacgtt ggagtcgcgg
      841 atcgacggcc acgcgcgggc gaagccagcc tcgatcgccg tcgcgtacga gcgcccctcc
      901 cgcatctcct ctttgagccg ctcgaatacg aggatgttcg cgtcgacggc catgccgacc
      961 gagagcacga acgctccgat gccggccagc gtcatcgtca ccgggatgag cttgaagatc
     1021 gccagcgaca gcagcgtgta cacgatcagc gccactgagg cgaggatgcc cggcacgcgg
     1081 tagtacagca ccatgaacag gatcacgatg agcaggccga tctcacccgc gacgacggac
     1141 tgccggacgg catcgtcgcc cagcgtggcg tcgacgttct gctcctggag tacggtcagc
     1201 tgcacgggaa gagcgcctga gttcagctgg atcaccaggt ccttggcccc ggtgcggccc
     1261 tccttggtcg ttgtcagcgg gaggcccgtg atctggccgc ggtcctcgat cacgccgttc
     1321 acgttcgggg acgccagcgc ctcgttgtcg aggaagatgc cgaggggctg cccgagcagg
     1381 cgctgcgtga cctgcttcga aagctgcgcg ccgtccgagt tccactcgaa gtccacgacc
     1441 gggttgccaa gctggtctgt accaacgaag gcgttcggct tcaggaaccg cgaggtcagc
     1501 ggcaccggtt tgccatccag ctcggcgacg gcctgtaccc actggccctg gtcgtcacac
     1561 ggctgcgcga cgtccgggaa cgggctctgc ccctgctgac agaactgcag cagcgccgtg
     1621 cgcccgagca ggtcgcgggc cttgtcgggc gttaggcccg ggagctgcac ggagatgttc
     1681 gagttgccct ggcgagtgat ctcagcctcg gacacgccgg aggagtcgac gcgcttgcgc
     1741 agcacccgga tggtgccgtc catcgcgtcg ccgacatcgc cttccgtgcc cgggggcagc
     1801 gaggcgtcga gcagcaggcg ggtgccgccc tggaggtcga ggccgagccg catcgtcttg
     1861 cgctcgaagc tcccgatcga aatgcccttt cccgccggcc atgggatggc cgaagggaac
     1921 cagcgctccg gctgactcgg ccacacgatg atgagcgcgg cgacggagag cacggcgatg
     1981 atggcgatgg gccacaggtg tgcgcgcgtg ggcatcagtt ggcgctcgcc ttccccgcat
     2041 cgtcggcctc ggtgaggtcc tcggatgcgt gtctcgatac cgattgcacc gcatctgcgg
     2101 tcgcacggag cagcacgccc ggcgcggcct cgagcgtaag ctccagcggg ccgcggtcgc
     2161 gtgtctggat gtcgcggatg accgcgaaga agcccccggt cgtcagcacc tcgtcaccga
     2221 tctccaacga cgagatgtcg cgtcggcggc gctgctgctg ctggatgacc ggacgcaaca
     2281 gaatgaaata gaacgcgagg aagatgagaa gcgcaaggaa gatgaactcg cccacggcta
     2341 ctcgtcgccg ccagcagcgg cggtccgttc cggctcggcc tgccttgccg cgactgctcg
     2401 ttcgtccacc ttgtagagat ccgaggggct gtcgagccgg tcgacgaaca gaaaaccgtt
     2461 gatgtgatcg acctcgtgct cgaacgcctg cgcgaggagg tcgtcctcgg cgcgcatccg
     2521 aacttcgcga ccgcgctcat cgagcgcccg caccacgacc ttctgcgagc gggtgatcgc
     2581 atgcgtccgg taccccggca ccgagaggca accctcgccc tggaggacgc gcgaccccgt
     2641 ccgcttcacg atgaccgggt tgatcagtgc gaagggctct tcgtcgggca tgccgaggac
     2701 gacaacacgc aacggcaccc cgatctgggg cgccgcgagg cccacaccct gggccgcata
     2761 catcgattcg atcatgtcca cgatcaggcg gcggatggag tcatccacct ggcgcacctt
     2821 cttcgcgggc tgtcggagta ccggatcgcc gaggtaccgg atcggacgca cagtcacgaa
     2881 accgattgta ggccacatcc ccggcagcgt ctccgaacgc tggtccgagg gcgccgaggc
     2941 agaccgcctc gacgtagact cggctcgtgc ctcgtcgcct cgtccgccgc agccgttcgc
     3001 cggcccgtcc tggggactgg gcgctggtcg cggtcgccaa agacgaggtg accgccgaag
     3061 aatggcagcg cctgctctcg ggcgagggca tcgaggcgca ggtgcgcatc gacgacccgg
     3121 cgcgggcggg catgccgggc cgcacggcgt ttccgctcgg ctacaccgag ccggggctgt
     3181 cgctcttctc gtatcccctc tacgtacgtc gccagcagcg ccgggaggca cgccagctgc
     3241 ttggagagca ggtcgaattc agtcgctccg ggctcagcgg gcaaaccgtc cttggcgccg
     3301 tcggcgtggt cgtggcgtcg ctcgcgatcg tcatcctcct ggtcctccga ggctcgtgac
     3361 cctgtctgcg gtaccatcca ctcgatgcgc gccctgctct gccgctcgct cgaagaaggc
     3421 atcgacggcc tgcgggtcga ggacattcct ccccccgggt tgtcgccgga cggcgtccgc
     3481 atcgaggtcc atgccgcggc gatgaacttc gccgacacgc tgctggtg
//
LOCUS       DAORPG010000135         3503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2349_length_3503_cov_3.599768, whole genome shotgun sequence.
ACCESSION   DAORPG010000135 DAORPG010000000
VERSION     DAORPG010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2349_length_3503_cov_3.599768"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PLX85_04090"
     CDS             <1..464
                     /locus_tag="PLX85_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HRC62385.1"
                     /translation="TSNDAFDMVAAAEDGRGIVRAMQRALKRGEVPLDAVDYINAHGT
                     GTPLNDRVETRAIRAVFGPQADRLMVSSTKSMIGHLMGAAGAVEAAVAVLTCARGVVP
                     PTINYTTPDPDCDLDYVPNEARTATVRYAMSTSVGLGGHNSAVVFGRWEGA"
     gene            464..1717
                     /gene="fabF"
                     /locus_tag="PLX85_04095"
     CDS             464..1717
                     /gene="fabF"
                     /locus_tag="PLX85_04095"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017325348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRC62386.1"
                     /translation="MVTQGPRRVVVTGIGMVTSVGIGRETTWQALLAAKNGIRSITLC
                     DPTDLESTIAGEVPDFDPLNYVDRKEARRMDRFVHLAIAASGEAIAHSGLDIKENAEE
                     IGVMIGSGIGGIATLEDQFHVLFEKGPGRVSPFLVPMFIPDMASGQVSIVFGARGPNY
                     NTVSACASGADALGAALETIKRGDAVAMLAGGAEATVTRMGIASFAASRALSTKRNHD
                     PEHASRPFDLERDGFVLGEGAATLVLEELEYARARGANILAEFVSYGQSADAFHVTQP
                     SENGEGAARAMRMALRKAGASASDIDYINPHGTSTPMNDRFETMSIKAVFGEAAGAIP
                     ISSTKSMVGHTLGACGGIEAAVTVLSIRDQRIHQTRNLEIPDPDCDLDYVPEGPRNLE
                     IDWAMSNSLGFGGHNSSLVFRRYHG"
     gene            1819..>3503
                     /gene="recJ"
                     /locus_tag="PLX85_04100"
     CDS             1819..>3503
                     /gene="recJ"
                     /locus_tag="PLX85_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRC62387.1"
                     /translation="MTTLVRPEPRILEGSGGRRWRIRQTPGLGPFEGAPWPALVSVLL
                     RHRGVHDLGQAHEYLGAPGELTNPKLMPNLDVAVERLARACLAHERVAILGDFDVDGV
                     TSTTILTEGLSDLGAIPLPYIPDRFSEGYGPNVDAVKALRARGASVLVTADCGTSAVR
                     EVAVANELGMDVIVIDHHTVPEELPDALALVNPKLQSSEYGSEPAAVGVAYKVVHDLY
                     DQLGRAYDPDEHRALVALGTVCDLAPMLAENRDLVRLGLGALRATRRPGLLALAEVAG
                     IDLADVDPDMCGWVLGPRLNAAGRMEHARISLELLLSRNLREAKDLAKRLETLNERRR
                     SETATACELATSLLTEAERTGPLVMVAAPELHAGIVGLVAARLVEQLQRPAIVMQVEA
                     GEARASCRSISAFDITALLRRNAHLFKRFGGHRAAAGFTIDAARLDEVRATLIEDAGR
                     HLDPADLVPTIDVDAELPLRHVNGDLLKWLSRLGPHGIGNETPTFMSPNVRVLDSRAV
                     GKDGSHLQFKLKEGAVTWRAIAFGNAANAVPDGECSDLVYTFRRDALRGTLQL"
BASE COUNT          564 a         1227 c         1187 g          525 t
ORIGIN      
        1 gcacctcgaa cgatgccttt gacatggtgg cggcggcgga ggacggccgt ggcatcgtgc
       61 gggccatgca gcgcgcgctc aagcgcggcg aggtgccgct ggacgcggtc gactacatca
      121 atgcccacgg gacgggcacg ccactcaacg accgcgtgga gacccgcgcg atccgtgcgg
      181 tattcgggcc tcaggccgac cgtctcatgg tgtcctcgac gaagtcgatg atcgggcacc
      241 tgatgggggc ggccggcgcc gtcgaggctg ccgtcgcggt cctcacgtgt gcgcgcgggg
      301 tggtcccgcc gacgatcaac tacaccacgc cggatccgga ctgcgacctc gattacgtgc
      361 ccaacgaggc acggacggct acagtccgct acgcgatgag tacgtcggtc gggctcgggg
      421 ggcacaactc cgccgtggtc ttcggccgct gggagggtgc gtgatggtga cgcaggggcc
      481 gcgacgggtg gtcgtgacgg gcatcggcat ggtgacgtcc gtaggcatcg gccgagagac
      541 gacctggcag gcgctgctcg ccgcgaagaa cggcatccgc tcgatcacgc tctgtgaccc
      601 gaccgacctc gagtccacca tcgccggcga ggttccggac ttcgacccgc tgaactacgt
      661 cgaccgcaag gaagcgcggc gcatggaccg cttcgtgcac ctcgcgatcg ccgcgagcgg
      721 cgaagcgatc gcgcactcgg gcctcgacat caaggagaac gccgaggaga tcggggtgat
      781 gatcggctcg ggcattggcg gcatcgcgac gctcgaggat cagtttcacg tcctcttcga
      841 gaaggggccg ggacgtgtct cgcccttcct cgtgccgatg ttcatccccg acatggcgtc
      901 gggtcaggtc tccatcgtgt tcggagcccg cggaccgaac tacaacaccg tgtcggcgtg
      961 cgcctcgggc gccgatgctc tgggcgcggc gctcgaaacg atcaaacgag gtgatgcggt
     1021 cgcgatgctc gcgggcggcg ccgaggcgac cgtgacccgc atgggcattg cctccttcgc
     1081 cgcgtcgcgc gcgttgtcga ccaagcggaa ccacgacccc gagcacgcca gtcggccgtt
     1141 cgacctcgag cgggacggct tcgtcctcgg cgagggcgcg gcgacgctgg tgctcgagga
     1201 actggagtac gccagggcgc gcggcgcgaa tatcctcgcg gagttcgtct catacgggca
     1261 gtcggccgat gccttccacg tgacgcagcc atccgagaac ggcgagggcg cggcgcgcgc
     1321 aatgcgcatg gcgctgcgca aggccggtgc gtcggcctcc gacatcgact acatcaaccc
     1381 ccacggcacg agcacgccga tgaacgaccg gttcgaaacg atgtcgatca aggcggtgtt
     1441 cggcgaggcg gccggggcca tccccatctc atcgaccaag tccatggtcg gccacaccct
     1501 cggcgcctgt ggcggcatcg aggcggccgt cacggtgttg tcgatccgcg accagcgcat
     1561 ccaccagacg cgcaacctcg agatcccgga cccggactgc gacctcgact atgtgccgga
     1621 ggggccgcgg aacctcgaga tcgactgggc gatgtcgaac tcgctgggct tcggcgggca
     1681 caactcctcg ctggtgttca ggcggtacca cggatagagt gcggaaccgc tcacttcgct
     1741 gagcgaacac ctgtactgga cacgccgtcc tcaccccgac agaatacgta gcactccccg
     1801 cgcgccctgg agtgcgatgt gaccacgctt gtccggcccg aaccgcgcat cctcgaggga
     1861 tcgggggggc ggcgctggcg gatccggcaa acgcccggac tcggaccgtt cgagggtgcg
     1921 ccgtggccgg cactcgtgtc ggtactgctg cggcatcgcg gtgtgcacga cctcggccag
     1981 gcacacgagt acctgggcgc gcctggtgag ctgacgaacc cgaagctcat gccaaacctg
     2041 gacgtcgccg tcgagcggct cgcgcgagcc tgcctcgcgc acgagcgcgt tgccatcctc
     2101 ggcgacttcg acgtcgatgg cgtgacctcg acgaccatcc tgaccgaggg cctctccgac
     2161 ctcggcgcga ttcctctgcc gtacattccc gaccgcttca gtgagggcta cggcccgaac
     2221 gtcgacgcgg tcaaagcact gcgggcgcgt ggcgcttcgg tgctcgtgac cgccgactgc
     2281 ggcacctccg cggtgcggga ggtggctgtc gccaacgagc tggggatgga cgtcatcgtc
     2341 atcgaccacc acacggtgcc ggaggagctg ccggacgctc tcgcgctcgt gaacccgaag
     2401 ctccagtcca gcgagtacgg cagtgaacca gccgccgttg gcgtcgccta caaggtcgtg
     2461 cacgacctct acgaccagct cggacgcgcg tacgaccccg acgagcaccg cgcgctcgtc
     2521 gcgctcggaa ccgtgtgcga cctcgccccg atgctcgccg agaaccgcga cctcgtccgg
     2581 ctgggcctcg gggcgctgcg cgccacccga cggccggggc tgctcgcgct cgccgaggtg
     2641 gccggcatcg acctcgccga cgtggacccg gacatgtgcg gctgggtgct gggtccgcgc
     2701 ctgaacgcag ccgggcgcat ggaacacgcg cggatctcgc tcgagctgct tctcagcagg
     2761 aacctccgcg aggcgaagga cctggcgaaa cgcctcgaaa cactgaacga acgacgtcga
     2821 tctgagaccg cgacggcctg cgagctggcc acctcgctgc tcacagaggc ggagcgcacg
     2881 gggccactcg tgatggtggc cgccccggag ctgcatgcgg gtatcgtggg gctcgtcgct
     2941 gcacgcctcg tggagcagct ccagcgcccc gccatcgtca tgcaggtcga ggcgggcgaa
     3001 gcacgcgcct cgtgccgctc aatctcggcc ttcgacatca ccgcgctgct gcggcgcaac
     3061 gcacacctct tcaagcgctt cggcggtcac cgcgccgcgg cgggcttcac aatcgacgcg
     3121 gcccgcctcg acgaggtgcg cgccaccctc atcgaggatg ccggccgtca cctcgatccc
     3181 gccgacctgg tgccgaccat cgatgtcgat gccgagctgc ccctgcgaca cgtcaacggc
     3241 gacctgctca agtggctgtc acggctgggg ccgcatggaa tcggcaacga gacccccacg
     3301 tttatgtcac ccaatgtccg cgtcctcgac agccgtgccg tcggcaaaga cggctcacac
     3361 ctccagttca agctgaagga aggcgccgtc acctggcgcg cgatcgcatt cggcaacgcg
     3421 gccaacgcgg tcccggatgg cgagtgctcc gacctcgtct ataccttccg ccgcgatgcg
     3481 ctgcggggca cgctccagct cga
//
LOCUS       DAORPG010000136         3503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2350_length_3503_cov_3.321346, whole genome shotgun sequence.
ACCESSION   DAORPG010000136 DAORPG010000000
VERSION     DAORPG010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2350_length_3503_cov_3.321346"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..755
                     /locus_tag="PLX85_04105"
     CDS             105..755
                     /locus_tag="PLX85_04105"
                     /inference="COORDINATES: protein motif:HMM:NF016163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HRC62388.1"
                     /translation="MERVAILGTGRMAPGIAAACVHAGVAHVAVLGRDRHLRRLVVVR
                     TDEEIEAAVERWRPALIAIDAPLALPEGRCCADPTCECARFGIYREIDRLCARLGYRP
                     FPTLLPSMVKLTLRGSALRERLVAGGEQVVEVYPGMVQDILGIPRKQAGLEGLTRGLR
                     RAGVRGIPRARRVTHDELDAVTCALAAVLHLEGRAQLLGSGVPVPFVAPLAPALPA"
     gene            777..1295
                     /locus_tag="PLX85_04110"
     CDS             777..1295
                     /locus_tag="PLX85_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRC62389.1"
                     /translation="MATPDDINELLAKLDEERGAFLAGVGDLSEEAAELRRPDLDGEA
                     GWSAKEQFSHLAEMELSYLVTAQRAVTGADIEQQRPRYPLARAHTATVAQHLEELARL
                     REQTVNYIHYLTPEEFDARVISGAFPEMSVLQWLRSYYRHDRMHLAQIEGRQSDYQPR
                     FVGGEPDQRRRG"
     gene            1288..2277
                     /locus_tag="PLX85_04115"
     CDS             1288..2277
                     /locus_tag="PLX85_04115"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HRC62390.1"
                     /translation="MADRPRVFVTRRLPGVAPLARLEEVATADVWEHARPPAAAEVTA
                     RAEGCTALLPMLTERIDGALLDACPTVHTVANMAVGYDNIDVPAATERGVLVTNTPDV
                     LTDTTADIAFALLLAAARRVTEGERKVRAGEWGTWQPDWLLGRDVHGATLGIVGPGRI
                     GRAVARRAAGFDMRVLYSGRREVTDFPGEYVSFEALLERSDFVSVHVPLTPETAGLFG
                     ADVFRRMKPTAIFVNTARGGVVDQQALRDALVRGEIAAAGLDVTTPEPLPPDAPLLEA
                     PNLVITPHLGSATAQTREAMADLAVDALRSVFAGERPRHLVNPDAWSHWRGRR"
     gene            2274..2948
                     /locus_tag="PLX85_04120"
     CDS             2274..2948
                     /locus_tag="PLX85_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRC62391.1"
                     /translation="MTNGPESDATGRFWLADVLWRLGGVQFGDFSLGRTVRHSPVYVN
                     PKLIIARPAALSRVATLIEDELRMAMAMRKQPVAPFDLIAGVPIGGLHIATALALQMR
                     TPLLYARPDGNVEEERPHIEGIYRPGQVALVVDDLATGGGSLVETVTRLRRAGLRVQD
                     ALVLIDREQGAARRLESIGVRLYSILSIEVLLTYLHSAERVPEDDYRRAMRYLATEGS
                     TRSEFD"
     gene            complement(2989..3204)
                     /locus_tag="PLX85_04125"
     CDS             complement(2989..3204)
                     /locus_tag="PLX85_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC62392.1"
                     /translation="MPDSPFQDYLDLVEAARELNIHPQSLRRLIKQRRVPAVLFAGKY
                     LIERDKLEMFKSNYDPRPGRKPIRRLL"
BASE COUNT          497 a         1331 c         1152 g          523 t
ORIGIN      
        1 gcccgtgatc cgccatccgg ggcagtgtgc ctgcgggcgc ggtcacgggc gtccgtagtg
       61 agtactgcct tgaccgcgga caacaccgcg ggtagaagcc ctccatggag cgggtcgcga
      121 tcctcggtac cggccgtatg gcacccggga tcgccgccgc gtgcgtccac gcgggcgtcg
      181 cgcacgtcgc cgtcctcggg cgcgaccgcc atctgcgccg gctggtggtg gttcgcacgg
      241 acgaggagat cgaggcggcg gtcgagcgct ggcgtcccgc gctgatcgcc atcgatgccc
      301 cgctcgcgtt gcccgagggc cgctgctgcg ccgacccgac ttgcgagtgc gcccgcttcg
      361 gcatctaccg cgagatcgac cgcctctgcg cccgcctcgg ctaccgcccc ttccccacgc
      421 tcctcccctc gatggtcaag ctcacgctcc gaggcagcgc gctgcgcgag cgcctggttg
      481 caggcggcga gcaggtcgtc gaggtgtacc ccggcatggt gcaggacatc ctcggtatcc
      541 cgaggaagca ggccggcctc gaggggctga cacgcgggct gcgccgtgcc ggcgtgcgcg
      601 gcatcccccg cgcccgccgc gtcacgcacg acgagctcga cgccgtcacc tgcgccctcg
      661 ctgccgtgct gcacctcgag ggccgtgcgc agctcctcgg ttccggcgtg cccgtgccgt
      721 tcgtcgcgcc gctcgcaccc gcgctccccg cctgattcag cgagtacaat cgcctcatgg
      781 cgacccccga cgacatcaac gagttgctcg cgaagctcga cgaggagcgc ggcgcgttcc
      841 tcgccggcgt cggcgatctg agcgaggagg ccgcggaact ccggcgtccc gacctcgatg
      901 gcgaggccgg ttggtccgcg aaggagcagt tctcgcacct cgcggagatg gagctgagct
      961 acctcgtcac ggcgcagcgc gccgtcaccg gcgccgacat cgagcagcaa cggccccgtt
     1021 accccctcgc cagggcgcac accgccaccg tcgcccagca cctcgaggag ctggcgcggc
     1081 tgcgcgagca gaccgtgaac tacatccact acctgacgcc cgaggagttt gacgcccgcg
     1141 tcatcagcgg cgccttcccg gaaatgtccg tcctgcaatg gctccggtcc tactaccgcc
     1201 acgaccgcat gcacctcgcc cagatcgagg ggcgtcagtc cgactaccag ccgcggttcg
     1261 tcgggggcga gcccgaccag cgccgccgtg gctgatcggc ctcgcgtctt cgtcacgcgc
     1321 agactccccg gcgtcgcgcc cctcgcacgc cttgaggagg tcgcgaccgc cgacgtctgg
     1381 gagcacgctc gccctccggc agccgccgag gtaaccgccc gcgccgaggg ctgcacggcc
     1441 ctgctgccca tgctcaccga gcgcatcgac ggcgcgctcc tcgacgcctg cccgaccgtc
     1501 cacaccgtcg ccaacatggc cgtcggctac gacaacatcg acgtgcccgc cgccaccgag
     1561 cgcggcgtcc tcgtcacgaa cacgccggac gtgctgaccg acaccaccgc cgacatcgcg
     1621 ttcgcgctgc tgctcgcggc ggcgcgccgc gtcaccgagg gtgagcgcaa ggtccgcgca
     1681 ggcgaatggg gcacgtggca gcccgactgg ctcctcggcc gcgacgtgca tggcgcgacg
     1741 ctcggcatcg tcgggcccgg ccgcatcggc cgcgccgtcg cgaggcgtgc cgccggcttc
     1801 gacatgcgcg tgctctattc gggtcgccgc gaggtgacgg acttccccgg cgagtacgtg
     1861 agcttcgagg cgctcctcga acgttccgac ttcgtctccg tgcacgtccc gctgacgccc
     1921 gagaccgctg gactcttcgg cgctgacgtc ttccgtcgca tgaagccgac tgccatcttc
     1981 gtgaacaccg cccgaggcgg cgtcgtggac cagcaagcgc tgcgcgacgc cctcgtccga
     2041 ggtgagatcg ccgccgcggg cctcgacgtc acgacgcccg agcccctgcc gcccgacgcc
     2101 ccgctgctcg aggcgcccaa cctcgtcatc acgccgcacc tcggtagcgc gacggcacag
     2161 acccgcgagg cgatggcgga tctggcggtc gacgcgctgc gctcggtctt cgcgggcgag
     2221 cgcccgcgcc acctcgtcaa tcccgacgcc tggtcacact ggagggggcg gcgatgacca
     2281 acggcccgga gtccgacgcc accggacgct tctggcttgc tgatgtgctc tggcgcctcg
     2341 gcggcgtgca attcggcgac ttcagcctcg gccgcaccgt gcgccactcg ccggtgtacg
     2401 tgaacccgaa gctcatcatc gcgcggccgg ccgcgctctc ccgtgtcgcg acgctcatcg
     2461 aggacgagct gcgcatggcg atggcgatgc gcaagcagcc cgtcgcgccc ttcgacctca
     2521 tcgcgggagt ccccatcggc ggcctgcata tcgccaccgc gctcgcgctg cagatgcgca
     2581 cgccgctgct ctacgcgcgt ccggacggca acgtcgagga ggagcgcccc cacatcgagg
     2641 gcatctaccg gcccggccag gtcgccctcg tggtggacga cctcgcgacg ggcggcggct
     2701 cgctggtgga gaccgtgacg aggctgcggc gggccgggct gcgcgtgcag gacgccctcg
     2761 tgctgatcga ccgtgagcag ggtgcggcac gccgcctcga gtcgatcggc gtccgtctct
     2821 actcgatcct gtcgatcgaa gtgctgctca cctacctgca ctccgccgag cgcgtgcccg
     2881 aagatgacta ccgccgcgcg atgcgctacc tcgccaccga gggctccacg cgctccgagt
     2941 tcgactagcg ctcgaaccta acttacgcgt tgcgagtggc gaccacgcct acagcagccg
     3001 acggatgggc ttccgccccg ggcgcgggtc gtagttcgac ttgaacatct cgagcttgtc
     3061 gcgctcgatg aggtacttgc ccgcgaacaa caccgccggc acgcggcgct gcttgatcag
     3121 ccgccgcagg ctctgcgggt gaatgttgag ctctcgggcc gcttcgacga ggtcgaggta
     3181 gtcctggaat ggggagtcag gcacaggtct cttccatctt tcgccgtcag agaagtgttc
     3241 atctgcgtac gcaagactac tgacgtgcct tctcagtgta aagcgcctac gcaattagag
     3301 tgacacgtcc gaggacgcgc ggcccgacct acagcgccgg caccgccgcg gcccgcacct
     3361 gctccacaac ccccggtagc tgctcgacga cataatcgat gtccgcctcg tcgagggccg
     3421 gccacaggct catccgcagc gagccgatgg ccagctcgat tgggacgccc atcgcgagga
     3481 gcacgtgcga gggctcccac gtc
//
LOCUS       DAORPG010000137         3490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2374_length_3490_cov_4.227074, whole genome shotgun sequence.
ACCESSION   DAORPG010000137 DAORPG010000000
VERSION     DAORPG010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2374_length_3490_cov_4.227074"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLX85_04130"
     CDS             complement(<1..485)
                     /locus_tag="PLX85_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62393.1"
                     /translation="MPPTEESARPDLAAELEAMWQHDQEVRAELAAAGSLFDGYHPRM
                     EQVHRANAARLREIIEAHGWPGRALVGVSAARAAFGVLNHAIGEPALQRLGLELLEAR
                     SAERDVDPIEVAMLADRIAFFEGRPQRYGTQFDWDDHGELRPWELDDADGVHLLRLLL
                     R"
     gene            complement(485..742)
                     /locus_tag="PLX85_04135"
     CDS             complement(485..742)
                     /locus_tag="PLX85_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HRC62394.1"
                     /translation="MTTLIEIVRMFVNVLIFAIFARSILTWFPIDRGGPVFQALDAIT
                     EPILQPLRRVVPLIGMIDITPMVAILLLYVLSTALGQSLSN"
     gene            complement(739..1545)
                     /gene="proC"
                     /locus_tag="PLX85_04140"
     CDS             complement(739..1545)
                     /gene="proC"
                     /locus_tag="PLX85_04140"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRC62395.1"
                     /translation="MRVGIIGGGFMGEAFLRGIVRSEFAGASDIAVAEKMPGRHLALS
                     EHGVRVTDDAQSACIGADLVLLAVKPQDVPAVAESLRGGLPASSVLVSIAAGVPLADV
                     QRYSAHRAVVRVMPNTPVAVGEGAAVCLIAAEVTAEQRLSVQRLLEAVCTAVVEVHDD
                     EEVDLATAIHGSGPAYVFLVIESMIDAAVRLGMKRADASALVLATVAGSARYAIATGT
                     HPAELKNAVTSPGGTTAAALRELEGAGLRSAFDDAIEAAFLRAQDLGELS"
     gene            complement(1575..2267)
                     /locus_tag="PLX85_04145"
     CDS             complement(1575..2267)
                     /locus_tag="PLX85_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRC62396.1"
                     /translation="MPSADVSTIAGRIADIRSSIARACERAGREAAEVTLIGVTKTFT
                     VDDVDEAIAAGLEDFGENRAQELLPKVEEASRRGLHARWHFIGHLQRNKVRDVLPHIV
                     ALHSLDSLRLAATIESEAERTGLERLRCYIEVNVAEEASKEGVPPAELPGLLSSLAVL
                     PHLEVAGLMTVAPQVPDAEQVRPVFRALRELASANGLRGLSMGMSEDYAVAIEEGATV
                     VRVGRALFGPRV"
     gene            2495..3430
                     /locus_tag="PLX85_04150"
     CDS             2495..3430
                     /locus_tag="PLX85_04150"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HRC62397.1"
                     /translation="MRNQKMLGGGLLAAFALMAAVACSSRDEAQTSGEVDSVAPAETV
                     ARSAGAPSTSDALVPPSKAAAVAEAGSSTAASAAVDVLGRKIIRNGTLALQVESVTGT
                     YEQVGAVAARLGGYVAEGSYSGAGDARTGRLVIRVPADNYDRAIAELQGLAKTVTSAS
                     SSSQDVTGEVSDLDAGLRNLRAVEAQYVAFLTQARTIPDLLQVQERLQSVRGDIERTE
                     ARLALLNRLSDLATITVQLAPIPVVMEVQHHKASPAGAAAEAWDASLETLRQVAVVGV
                     AVVVYSWWLMPGIAAAAYLGLRHMRGRRVLTGAAS"
BASE COUNT          579 a         1210 c         1163 g          538 t
ORIGIN      
        1 tgccgtagca gcaaccgaag gagatgcacg ccatccgcat cgtcgagctc ccacggacgc
       61 agttcgccgt ggtcatccca gtcgaactgc gtcccgtagc gttgcgggcg cccctcgaag
      121 aacgcgatgc gatcggcgag catcgccacc tcgatcgggt ctacgtcgcg ctcggcggat
      181 cgcgcctcga gcagttcgag gcccaaccgc tgcagagcgg gctcaccgat ggcatggttc
      241 aagactccga aggccgcccg agcggcggaa acccccacga gtgcgcggcc cggccacccg
      301 tgtgcctcga tgatctcgcg caggcgtgcc gcatttgcgc ggtgcacctg ctccatgcgg
      361 gggtgatagc cgtcgaagag tgatccggcg gcagcgagtt cggcacgcac ctcctggtcg
      421 tgctgccaca tggcctcgag ctcggcggcc aggtcgggcc gcgcgctttc ctcggtcggc
      481 ggcatcagtt cgagagcgac tggccgaggg cggtcgaaag gacgtagagg aggaggatgg
      541 caaccattgg ggtgatgtcg atcatcccga tcagcggcac cacacgccgc agaggctgca
      601 ggatcggctc ggtgattgcg tccagcgcct ggaacaccgg ccctccgcgg tcgattggga
      661 accaggtcag gatcgaacgg gcgaagatcg cgaagatcag cacgttcacg aacatccgca
      721 cgatctcgat gagcgtcgtc acgacagctc ccccaggtcc tgtgcgcgca ggaaggcggc
      781 ttcgatcgcg tcatcgaagg ccgaacggag tcccgcccct tcgagctcgc gcagcgccgc
      841 cgcggtcgtg ccgccgggcg atgtgacggc attcttcagc tcggccgggt gcgtccccgt
      901 cgcgatcgcg tagcgcgccg acccggccac cgtcgccagc accagtgctg aggcgtccgc
      961 acgcttcatc ccgaggcgca ccgccgcgtc gatcatggac tcgatgacaa ggaagacgta
     1021 ggcgggaccc gatccatgga tggccgtcgc gaggtcgacc tcttcgtcgt cgtgcacctc
     1081 gaccaccgct gtacatacgg cttcgaggag ccgctgcacc gacagacgct gctcggcggt
     1141 cacctcggcg gcaatgaggc agacggccgc cccttcgccg accgcaaccg gtgtgttcgg
     1201 cataacccgc acgaccgcgc gatgggcgct gtaccgctgg acgtccgcga gtgggacacc
     1261 cgcagcgatc gaaacgagca cgctgcttgc tggcagtccg ccacgcaggc tctcggccac
     1321 cgccggcacg tcttgtggct tcacggccag cagcacgagg tccgctccga tacaggcgct
     1381 ctgcgcgtcg tccgtcaccc ggacgccatg ctccgagagc gcgaggtgtc ttccaggcat
     1441 cttctccgca accgcgatgt cactcgcgcc cgcgaattcc gagcgcacga tcccgcgcag
     1501 gaaggcctcg cccatgaagc cgccgccgat gatgccgacc cgcactcgaa cgcctcccgc
     1561 cgtgctcgtg tagctcagac ccgaggccca aagagggcgc gaccgacgcg caccaccgtg
     1621 gccccttctt caatcgcgac cgcgtagtcc tctgacatcc ccatcgacag gccgcgcaga
     1681 ccgttcgcac tcgccagctc gcgcagcgcg cggaagaccg ggcgtacttg ctcggcgtcc
     1741 ggcacctggg gcgcgaccgt catcagcccg gccacttcga ggtgcggcag cacggcgaga
     1801 ctgctcagga gaccgggcag ctccgcgggc gggacccctt ctttggaggc ctcttccgcg
     1861 acattcacct cgatgtagca acgcagccgc tcgaggccgg tgcgctccgc ctccgactcg
     1921 atcgtggcag ccaggcgaag cgaatcgagg gaatgcagcg ccacgatgtg ggggagtaca
     1981 tcccgcacct tgttgcgttg cagatggccg atgaaatgcc agcgggcgtg caacccccgt
     2041 cgagaggcct cttcgacctt gggcagtagc tcctgcgctc gattctctcc gaagtcctcg
     2101 aggccggcgg caatcgcctc gtcgacatcg tcgacggtga acgtcttcgt gacgccgatg
     2161 agcgtcacct ccgcggcctc tcggcccgcc cgctcgcacg cgcgcgcgat tgagctgcga
     2221 atgtccgcga tgcggcccgc aatcgtagag acgtcagcgg agggcatagg gagagcgtac
     2281 gccgtgcgaa gagggacgga ctagtaggcg ctgtggaccg ctcgaggcga cgtcaggagg
     2341 aaagcgacgc cggatccaca gcgccttaca taagtatact gctggtgccg ggcgctcgga
     2401 tcgctctttc cataattcct cggaccgcgg acccatcggg aactgttgga cggcccctct
     2461 cgtctgaatc gacatcgacg gaaggaggca ttcgatgcgc aaccagaaga tgcttggcgg
     2521 tggtctgctt gcggccttcg cgctcatggc ggccgttgcg tgttcgtcac gcgacgaggc
     2581 gcagacgagc ggagaagttg attcggtcgc gccggcagag acggtggctc gcagcgccgg
     2641 cgcaccgtcc accagtgacg ccctcgtacc gccgagcaag gcggccgcag ttgccgaggc
     2701 gggaagcagt acggcagcct cggcggccgt cgacgtcctc ggtcgcaaga tcatccgcaa
     2761 tggcactctc gcgctgcagg tcgaatccgt gaccggcacc tacgagcagg tgggtgccgt
     2821 ggccgcccgg ctcggcgggt acgtcgccga gggctcgtac tccggcgccg gcgatgcgcg
     2881 caccggccgg ctcgtgatcc gtgtcccggc ggacaactac gaccgtgcga tagcggagct
     2941 gcaagggctc gcgaagaccg tcaccagcgc ctcgagcagc agccaggatg tcaccgggga
     3001 ggtcagcgac ctcgacgccg gactccgcaa cctccgcgcc gtcgaggcgc agtacgtggc
     3061 attcctcacg caggcacgga cgatcccgga tctgctgcag gtgcaggagc ggctccagag
     3121 cgtccgtggc gacatcgaac gcaccgaggc acgcctcgcg ctgctgaacc gtctctcaga
     3181 cctcgcgacg atcacggtgc agctggcgcc tatccctgtg gtgatggagg tgcagcacca
     3241 caaggcgtcg ccggctggcg ccgctgccga ggcctgggac gcttcgctcg agacgctgcg
     3301 tcaggtcgcc gtggtgggtg tcgcggtcgt cgtctactca tggtggctca tgcccggcat
     3361 cgccgccgcg gcctacctcg gcctgcgtca catgcgcgga cgccgcgtcc tcaccggcgc
     3421 cgcgtcctga tgcaaaggga ggggccattc gggattccga ttggccctcc gcctacgatg
     3481 tgacggagag
//
LOCUS       DAORPG010000138         3459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2428_length_3459_cov_3.514689, whole genome shotgun sequence.
ACCESSION   DAORPG010000138 DAORPG010000000
VERSION     DAORPG010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2428_length_3459_cov_3.514689"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..927)
                     /locus_tag="PLX85_04155"
     CDS             complement(166..927)
                     /locus_tag="PLX85_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC62398.1"
                     /translation="MRSSIIGEHVPDAARLGNPSFVWRSGQERRLAIIERYVSLEGRR
                     VLDLGCGLGEYVRAFERHGAEAFGCDIEQPRLVQARDRGTPNLLVAAGERLPFRDGSL
                     DVVVLNEVIEHVQDDTSTMCEVARVLAPGGVAVIYAPNRLYPFETHGIFLGNKRYIFG
                     NIPLVNWLPNPLRNRLVPHARAYTAGGIERVARQRGLRLVHHSYVYPGFDNIAARVPV
                     LARLLRWSCYRAEDSALRRFGLSHLLVLHREEATV"
     gene            1073..1360
                     /gene="gatC"
                     /locus_tag="PLX85_04160"
     CDS             1073..1360
                     /gene="gatC"
                     /locus_tag="PLX85_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRC62399.1"
                     /translation="MPLTADEVRHIARLARLGLSPEEVERFRDQLSTILDHFQGLNAF
                     DTEGVPPTAQSFDLTNVERDDRARPSADRSEVLANAPRTEGAYVRVRAVLD"
     gene            1409..2884
                     /gene="gatA"
                     /locus_tag="PLX85_04165"
     CDS             1409..2884
                     /gene="gatA"
                     /locus_tag="PLX85_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRC62400.1"
                     /translation="MLDLPAHEQAARLRAGDLTARQLAEAALARVHAQDASLGAYITF
                     MDEEALAQADEADARLRSGNAGPLTGIPIGVKDLLSTKGTLTTAASRMLEGFRPIIDC
                     TVVARLREAGAVFVGKTNLDEFAMGSSTEHSAFAQTHNPWDLARVPGGSSGGSAATVA
                     AGGVPLSLGTDTGGSIRQPAALCGILGLKPTYGRVSRFGVIAFASSLEQVGPFGRDAE
                     DVALLLQAIAGLDPNDATTAPVPVPDYREEFARGIEGLRVGVPRDFFVEGMEDGVRQA
                     VEDAIAVFERNGAVVDRDVALPTVSAALPVYYIIAPSEASANLARYDGVKYGFSYHDA
                     ASMWEEMEQTRGRGFGDEVKRRIMIGTYALSAGYYDAYYLKAQKVRTLIRRDFDAALS
                     NHDLLLTPTAPTVAFRLGAKTDDPLAMYLNDLCTLPVNIAGNPGMSVPCGFSEGLPVG
                     LQLIGRPFEEATLLRAAHAYGTFTDWHLRRPPALASDGAGA"
     gene            2881..>3459
                     /locus_tag="PLX85_04170"
     CDS             2881..>3459
                     /locus_tag="PLX85_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRC62401.1"
                     /translation="MTAEGVGTGHVARRGRIAETVSSLPPSGIREFFELLDHTEGVIS
                     LAIGQPDFTTPAQITQAAADSMLAGNTGYTSNYGLIELREALSRQLERLYGVRYSPER
                     ELLLTTGVSEALDIAVRALVDHGDEVLVPEPGYVAYEPVILLAGAKYVPVPTSAEDRF
                     MVTAEALREHLTPATKAILIGYPCNPTGAVMDR"
BASE COUNT          523 a         1254 c         1180 g          502 t
ORIGIN      
        1 cgtacaggcc cgccgcgccg gtgatgccga ggccgaggac gaagagggcg acgagcgcgc
       61 cgccgatgat ggtgagcgct cggcggcgcc ccccgcctcc cgtgggcgct tcgccgttgg
      121 cgatgcgccg cagcaggcgc cggcgtcgta tgcgttgtcc tgcggtcata cggtcgcctc
      181 ctcccggtgc agcacgagca ggtgggagag cccgaaccgc cgcagggccg agtcctcggc
      241 gcggtagcac gaccagcgca gcaaccgcgc gagcaccggc acccgggccg cgatgttgtc
      301 gaacccgggg tagacgtagc tgtgatggac aaggcgcagg cctcgctgcc gcgcgacgcg
      361 ctcgatgccg cccgcggtgt aggcgcgagc gtgcggcacc agccggttgc gcagcgggtt
      421 cggaagccag ttgacgagcg ggatgttccc gaagatgtac cgcttgttgc ccaggaagat
      481 gccatgggtc tcgaacgggt agaggcggtt cggtgcgtag atgaccgcca ctcctccggg
      541 cgccagcacg cgagccacct cgcacatcgt ggaggtgtcg tcctgcacgt gctcgatcac
      601 ctcgttgagc acgaccacgt cgagcgagcc atcgcggaac ggcagccgtt caccggccgc
      661 cacgaggagg ttcggcgtcc cgcggtcccg agcctggacg agccgcggct gctcgatgtc
      721 gcagccgaag gcctccgcgc cgtggcgctc gaaggcacgg acgtactcgc cgaggccgca
      781 gccgaggtcg agcacgcggc ggccctcgag ggacacatag cgctcgatga tcgcgagccg
      841 gcgctcctgg ccggatcgcc agacgaagct cgggttgccc aggcgggcgg catcggggac
      901 gtgctcgccg atgatgctgc tcctcacggc gcgagtgatc ggcctgccgg caatggtggg
      961 atggacatgg tacgaaacac agccagcgcc ctacaccccg aggcccggca ccgttctggt
     1021 gcgctcgggg ggctgcacct atagttactc atacgggacc cggaggaacc acatgcccct
     1081 caccgctgac gaggtgcggc acatcgcccg cctcgcccgc ctcggccttt ctcccgagga
     1141 agtcgagcgc ttccgtgacc agctctcgac gatcctcgat cacttccagg gtctaaacgc
     1201 cttcgacacc gaaggcgttc ccccgacggc gcagtccttc gacctgacca acgtcgaacg
     1261 tgatgaccgc gcccgcccct cggccgatcg ctccgaggtc ctggcgaacg caccccgcac
     1321 ggaaggcgcc tacgtgcgcg tccgcgcggt gctcgactag gcggtcccgg gcatgtcctc
     1381 gagcagtcct tcggctgacg tattcgctgt gctcgacctg cccgcgcacg agcaggcagc
     1441 ccgtctgcgg gcgggcgatc tgacggcgcg tcagctcgcc gaggcggccc tggcgcgagt
     1501 gcacgcgcag gatgcatcgc tcggcgccta catcaccttc atggacgaag aagcgctggc
     1561 ccaggccgac gaggcggacg cgcggctgcg ctccggcaac gccggcccgc tgaccggcat
     1621 cccgatcggc gtgaaggacc tgctctcgac gaaggggacc ctcacgacgg ccgcctcgag
     1681 gatgctcgag gggttccggc cgatcatcga ctgcaccgtc gtcgcccggc tgcgcgaggc
     1741 cggcgccgtc ttcgtcggca agacgaacct cgacgagttc gcgatgggtt catcgaccga
     1801 gcactcggcg ttcgcgcaga cgcacaaccc gtgggacctc gcacgcgtcc cgggcggatc
     1861 gtccggcggg tccgccgcga cggtcgccgc gggaggcgtg ccgctctccc tggggacgga
     1921 cacgggcggc tcgatccgtc agcccgcggc gctctgcggc atcctgggac tgaagccgac
     1981 ctacggccgc gtcagccgct tcggcgtgat cgcgttcgcc tcgagcctcg agcaggtcgg
     2041 ccccttcggg cgggacgccg aggacgtcgc gctcctgctg caggccattg ccggcctcga
     2101 tccgaacgac gccacgacgg ccccggtgcc cgtgcccgac taccgcgagg agttcgctcg
     2161 cgggatcgag ggcctgcggg tcggagtgcc gcgcgacttc ttcgtcgagg gtatggaaga
     2221 cggcgtgcgc caggccgtcg aggacgccat cgccgtgttc gagcgcaacg gcgcggtcgt
     2281 cgaccgcgac gtcgcgctgc cgacagtgtc cgcggcgctg ccggtctact acatcatcgc
     2341 cccgtccgag gcttccgcga atctcgcccg ttatgacggc gtcaagtacg gtttctccta
     2401 ccatgacgcc gcctcgatgt gggaagagat ggaacagact cgtggccggg gtttcggcga
     2461 cgaggtgaag cggcgcatca tgatcggcac ctacgccctc tccgccgggt actacgacgc
     2521 gtactacctg aaggcacaga aggtccgcac gctcatccgc cgcgatttcg acgcggccct
     2581 gtcgaaccac gacctgctgc tcacgccgac ggcgccgacc gtcgccttcc gcctcggcgc
     2641 gaagaccgac gacccgctcg cgatgtacct caatgacctc tgcacgctgc ccgtaaacat
     2701 cgcgggcaac ccgggcatgt ccgtgccctg cgggttctcc gagggcctgc cggtcgggct
     2761 gcagctgatc gggcgaccct tcgaggaggc gaccctgctg cgcgccgcgc acgcgtacgg
     2821 cacgttcacc gattggcacc tgcgccggcc acctgcgctc gcctcggacg gagcgggcgc
     2881 gtgacagccg agggcgtggg caccggacac gtcgcgcgac gcggccgcat cgccgagacc
     2941 gtctcgagcc tgccgccctc cggcatccgc gagttcttcg agctgctcga ccacaccgag
     3001 ggcgtgatct cgctcgcgat cgggcagccc gacttcacta cgcccgcgca gatcacccag
     3061 gcagcggcgg actcgatgct cgccgggaac accgggtaca cgagcaacta cggactcatc
     3121 gagctgcgcg aggcgctcag ccgccagctc gaacgcctct acggcgtccg ctactcgccg
     3181 gagcgcgagc tgctgctcac cacgggcgtc tctgaagccc tcgatatcgc cgttcgggcg
     3241 ctggtcgacc acggcgacga agtgctggtg ccggagcccg gctacgtggc gtacgagccg
     3301 gtcatcttgc tcgcgggcgc caagtacgtc cccgtcccga cgagcgccga ggatcgcttc
     3361 atggtgaccg ccgaggcgct gcgcgagcac ctcacgccgg ccacgaaggc catcctcatc
     3421 ggctacccgt gcaatcccac gggggccgtg atggaccgc
//
LOCUS       DAORPG010000139         3457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2432_length_3457_cov_3.626984, whole genome shotgun sequence.
ACCESSION   DAORPG010000139 DAORPG010000000
VERSION     DAORPG010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2432_length_3457_cov_3.626984"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLX85_04175"
     CDS             <1..261
                     /locus_tag="PLX85_04175"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRC62402.1"
                     /translation="GGVSPVLELAALLSINLGIINLLPLPMLDGGRVFFLLLEVLRGG
                     RRIAPEKEAMVHLVGFVVFIALAIVVTFADVSRIVNGESLFR"
     gene            317..784
                     /locus_tag="PLX85_04180"
     CDS             317..784
                     /locus_tag="PLX85_04180"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HRC62403.1"
                     /translation="MTLSAIEIRQLPPQPTACIRRSLRYSQTSTIPELIGRVAEALAE
                     AGLELVGRPYTRVLSLGLLSMEVEVGWPVPAPFAGRGEVTAGELPGGPAAVASYFGPY
                     EDIRPAYEAIAAWCRDHGRAVAGSPWESYCTDPGEEPDSAKWRTDVAFPLRDT"
     gene            846..1439
                     /locus_tag="PLX85_04185"
     CDS             846..1439
                     /locus_tag="PLX85_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62404.1"
                     /translation="MATHGAAGSTSRFSAWRPGAFAAALAVLGALTAAAVASMLWPDV
                     EDRRDVVVQFDPGGPVAQPFQLFDLDGWLVRQPDGSVRAFSAAEARTACGIFFVSRSD
                     RYLGLTGAARRGEQGFFTDHCWGSTWTLEGAITFGPSWRGMDEFEVRQSTGGRVSLDL
                     SRVRLGMCARGAEDLDCSLPGHARYRRPRAGHAWSFW"
     gene            1439..2068
                     /locus_tag="PLX85_04190"
     CDS             1439..2068
                     /locus_tag="PLX85_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62405.1"
                     /translation="MGAMAGDGAVHRSSRSAWRRLTFGLALLGLAVLVAVAVASMLWP
                     LVEDRSNVDVPFEARGAVAQRFSAFGLDGWLVRQADGSVRAFSAADAHLRCRVEFIPV
                     GHPLYAARSTWKGLERGMFVDRCMHSLYDADGKPLYGPAPWGLDEFLVHEVHGGRLYL
                     DLSRVMRGECSPGLSSTQYCSVPGRPRFGEPARVDEGYTSRFIWPVAIR"
     gene            2103..3260
                     /gene="ispG"
                     /locus_tag="PLX85_04195"
     CDS             2103..3260
                     /gene="ispG"
                     /locus_tag="PLX85_04195"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HRC62406.1"
                     /translation="MADIHLIETKPRNETRAVRVGNVTIGGGAPIAVQSMAATKTQNI
                     EATVRQVQLLESAGADLVRIAVDSRKDVEALAEIRGRTSAVLSVDLQENYRLAEFVAP
                     HVNKIRYNPGHLYHHEREKPVRDKVAFIAEIAQRNDCALRVGVNCGSVDPVMTAKFGD
                     DDVGAMVASALEHCAILDDLGFTRYVVSMKDSDWKKVVEGNQRFAAQRPDVPLHLGVT
                     EAGMPPEGIIKTRVAFEQLIPQGIGDTIRVSLTLPFERKGEEIAVGREILADIAAGRF
                     RSVPPRFGEGLNIISCPSCSRVENEAFIELAQHVKEMAAYAQDYAITIAVMGCRVNGP
                     GETDDADLGLWCGPAAVNLKRGEDSLGAYPYDEVLDRLRVELDAIIEARRR"
     gene            3343..3457
                     /locus_tag="PLX85_04200"
                     /pseudo
     CDS             3343..3457
                     /locus_tag="PLX85_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665612.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
BASE COUNT          498 a         1121 c         1259 g          579 t
ORIGIN      
        1 ggcggcgtct cgcccgtcct cgagctggct gcgttgctgt cgatcaacct cggcatcatc
       61 aacctgctgc cgctgccgat gctcgacggc ggacgggtgt tcttcctgct cctcgaggtg
      121 ctgcgcggcg gccgccgcat cgcgccggag aaagaggcga tggtgcacct cgtggggttc
      181 gtggtgttca tcgcgctcgc gatcgtcgtg accttcgcgg acgtctcccg catcgtgaac
      241 ggcgagtcgc tgttccggta acgagcccgc gaggcgacac gtcgcccctc gacgtccgcg
      301 cgcggcaggg cgctcgatga cgctctccgc gatcgagata cggcagttgc cgccgcaacc
      361 gacggcatgt atccgccgca gcctccgtta cagtcagacc tcgacgattc cggagctgat
      421 cggcagggtc gccgaagcgc tcgcggaggc cgggctcgag ctcgtgggcc ggccatatac
      481 acgggtgctc tcgctcggcc tgctctcgat ggaggtggag gtcggctggc ccgtaccggc
      541 gccattcgcc gggcggggcg aggtgactgc gggggagttg ccgggcggcc cagccgccgt
      601 ggcgtcgtac ttcggcccct acgaggacat ccggcccgcc tacgaggcaa tcgccgcctg
      661 gtgcagggat catggccgcg cggtcgccgg ttcgccctgg gagtcctact gcacggaccc
      721 cggcgaggag ccagactccg cgaagtggcg gactgatgtc gcgttcccac tgcgggacac
      781 gtagtcagcg agcttcatcc gtacggatga ggcggcgccg gtcgcgccgg ctcacactcc
      841 gaggcatggc gacacatggg gctgcgggca gcacatcgcg cttcagcgcg tggcgaccgg
      901 gcgcattcgc ggcggccctt gccgtgctcg gcgcactcac cgcggcagcg gttgcctcga
      961 tgctctggcc ggacgtcgag gaccgtcggg atgtggtggt gcagttcgat ccgggcggtc
     1021 cggtcgcgca gccgtttcag ctgttcgacc tcgacgggtg gctggtgcgc cagccggatg
     1081 ggagcgtgcg tgcgttctcc gcagcggagg cgcggacggc atgcggcatc ttctttgtga
     1141 gtcgcagtga tcggtacctt gggctgacag gcgcggcccg tcggggtgag cagggcttct
     1201 tcaccgacca ctgctggggc tcgacgtgga cactcgaagg tgcgattacc ttcgggccga
     1261 gctggcgggg gatggacgag ttcgaggtcc gtcagagcac gggcggacgc gtgagtctcg
     1321 acctgagccg tgtgcgcctc gggatgtgcg cacgcggggc cgaggacctg gattgctcgc
     1381 ttcccggtca cgcgcgctat cgacggccac gggccgggca tgcctggtcg ttctggtgat
     1441 gggtgcgatg gccggtgacg gcgcggtgca ccgctcgtcg cgatcggcgt ggagacgact
     1501 caccttcgga ctggcgctgc tcggtctggc cgtgctggtc gccgtcgcgg tcgcctcgat
     1561 gctctggccg ttggtcgagg accgctcaaa cgtggatgtg ccgttcgagg cgagaggagc
     1621 cgtcgctcaa cggttctcgg cgtttggcct cgatgggtgg ctggtgcggc aggccgatgg
     1681 gagtgtgcgg gcgttttcgg ccgctgacgc gcacctccga tgccgtgtcg agttcattcc
     1741 ggtggggcac ccactctacg ccgcgcgcag cacatggaag ggtctcgaac gggggatgtt
     1801 cgtcgaccgc tgcatgcact cgctctatga cgcggacggc aaaccactgt acgggccggc
     1861 gccgtggggt ctcgatgagt tcctcgtgca cgaggtacac ggcgggcgtc tgtacctcga
     1921 tttgtcacgg gtgatgcggg gcgagtgctc ccccggcctt tcgtcgacgc agtattgttc
     1981 ggtgcccgga cgaccgaggt tcggcgaacc agctcgtgtc gatgaggggt atacgtcgcg
     2041 gttcatctgg ccggttgcca tccggtagcc aagcccggcc ccgacatgcg aggatcgaag
     2101 gaatggcaga catccacctc atcgagacca agccccgcaa cgagacccga gccgtgcgcg
     2161 tcggcaacgt cacgatcggc ggcggagcgc cgatcgccgt gcagtcgatg gcggcgacca
     2221 agacgcagaa catcgaggcg accgtgcggc aggtgcagct cctcgagtcc gccggggccg
     2281 atctcgtgcg catcgccgtc gactcgcgca aggacgtcga ggcactggcg gagatccgcg
     2341 ggcgtacctc ggccgtgctg tccgtggacc tgcaggaaaa ctaccgcctg gcggagttcg
     2401 tcgccccgca cgtcaacaag atccgctaca acccggggca cctctaccac cacgagcgcg
     2461 agaagccggt gcgcgacaag gtcgccttca tcgcggagat cgcgcagcgc aacgactgcg
     2521 ccctgcgtgt cggggtgaac tgcggctcgg tggacccggt gatgaccgcg aagttcggcg
     2581 acgacgatgt cggcgcgatg gtcgccagcg ccctcgagca ctgcgcgatc ctcgacgacc
     2641 tcgggttcac ccgctacgtg gtctcgatga aggactcgga ctggaagaaa gtcgtcgagg
     2701 gcaatcaacg ttttgcggcc cagcggccgg acgtccccct gcacctcggc gtgaccgagg
     2761 cggggatgcc gcccgagggc atcatcaaga cgcgcgtcgc cttcgagcag ctgatcccgc
     2821 agggcatcgg cgacacgatc cgcgtctcgc tgacgctgcc gttcgagcgc aaaggcgagg
     2881 agattgccgt tgggcgcgag atcctcgcgg acatcgccgc gggacggttc cgctccgtgc
     2941 cgcctcgatt cggcgagggg ctgaacatca tctcgtgccc ctcgtgctct cgcgtggaga
     3001 acgaggcgtt catcgagctg gcgcagcacg tcaaggagat ggccgcgtac gcgcaggact
     3061 acgcgatcac gatcgcggtg atgggctgcc gcgtgaacgg ccccggcgag accgacgacg
     3121 cggacctggg tctgtggtgc gggccggcgg cggtgaacct caagcgcggc gaggattcgc
     3181 tgggcgccta cccgtacgac gaggtgctgg accggctgcg cgtggagctc gatgcgatca
     3241 ttgaggcgcg ccgcaggtag gtcctgcccg ggccgggctt cgatcccctc ccccctttgc
     3301 gggggagggt gagggagggg gcttcccgtc accacgaccg gacgcgcgag agcactgcgg
     3361 gcccatgcca ccgacgcgga acgcgcgctg tggcaggcct tgcgtcggcg gcggtttgcg
     3421 ggccacaagt ttcgccggca gcagccccta ggccatt
//
LOCUS       DAORPG010000140         3426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2477_length_3426_cov_3.136755, whole genome shotgun sequence.
ACCESSION   DAORPG010000140 DAORPG010000000
VERSION     DAORPG010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2477_length_3426_cov_3.136755"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..541
                     /locus_tag="PLX85_04205"
     CDS             119..541
                     /locus_tag="PLX85_04205"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HRC62407.1"
                     /translation="MFTDGSCSGNPGPGGWGAVRVEGGRVVVEEHGAEAATTNNRMEL
                     TAMIAGLRMLGPGEAGDVYSDSRLVVQTLTQWAAGWERRGWKRTTGEVKNLELVQEAY
                     ALAKARPRARIQWIRAHDGSRWNEYADALSTAYLRSEV"
     gene            complement(574..1581)
                     /locus_tag="PLX85_04210"
     CDS             complement(574..1581)
                     /locus_tag="PLX85_04210"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRC62408.1"
                     /translation="MRFRSVRTLLALQLLLSIGTLALLFWRVDIVGAVRQLPDVRYGW
                     LVPGLLMFTASKGIHAYRWRYFLRHHEEIPTRHLFALFLVSNLANAVIPFRAGDLLRI
                     ELPNRRFGVARAEMASTVLVVESLLDGVTFVILLTTGIALLDLPEVLRSTLLTLAVIV
                     LSIFAVTVLAARYGTAWDPTHSRVLRPVPARARPFIGRRFVETVAGLASLRTTSATAV
                     AIGISICAWVTEVGVYWFIGHAFGLGMTVAEALVVTIAANLAVAVPVTPWNVGPYEVV
                     VTEALVAMGAPRDTASGYAIGSHLLLLAWIGVTGFVAMWSLDLSLRDLVHRSAQAPPA
                     A"
     gene            complement(1639..2070)
                     /locus_tag="PLX85_04215"
     CDS             complement(1639..2070)
                     /locus_tag="PLX85_04215"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRC62409.1"
                     /translation="MGADIDPALLPWIAPEETPFWRYLGIRFVEAEGGRAVVAMAMRP
                     EFGTVNRPDVVHGGAIATLIDAAAGTAVRTIRSADEPAWRGMSTTDLNISYLDAATSD
                     LTAEGRVLRSGRTIAWAQVDVRDAKGVLVAVGRVTAAIRRA"
     gene            complement(2082..2306)
                     /locus_tag="PLX85_04220"
     CDS             complement(2082..2306)
                     /locus_tag="PLX85_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62410.1"
                     /translation="MRDQLIGLLAERIGLNEDQAGQAVDTILGFVQENPQQLLGLLGG
                     NSEQLASLLGDNSPVDLDDLTGSLGKLFGG"
     gene            2407..>3426
                     /locus_tag="PLX85_04225"
     CDS             2407..>3426
                     /locus_tag="PLX85_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HRC62411.1"
                     /translation="MQREVIVVGAGPAGSTAAREIARRGHEVLLVDRAKFPRDKPCGG
                     GVTVRCAGLLPFDIEPVVEHVVTGAILQGPNGKTKVRESGAPLTFMTQRRRLDAFLAE
                     RAQEAGAEFRDGLAVRELTGTAEDFEVRLADGSEVRARVVIGADGANGVVGTHLGFDH
                     PLESAVALEGNLPCPDGPPPWLAGKVALSLGWLPGGYGWLFPKGDHVNVGVGGWKAVV
                     GGQLRDSLEAYARSFGWEASMLTNLRGHHLPMQRPGTDVAGLAAAVIGDAAGLVDPLS
                     GEGIYAAIASGVAVAPAAVDYLEGRASSLSGYQLAVERELLPDLVASRALMEIFHAAP
                     GPFSWL"
BASE COUNT          508 a         1072 c         1417 g          429 t
ORIGIN      
        1 tgggcgaggc ggcctcgcgc cggcctcggg cggcccgcgt ggcaactcgg cgacccgttc
       61 gcagctgctg accacggcgc agatactcga acggtacgtg gccgggccgg acaccggtgt
      121 gttcacggac gggtcgtgca gcggcaaccc ggggccaggc ggctggggcg cggtgcgcgt
      181 cgagggcggg cgcgtcgtcg tcgaggagca cggcgccgag gccgcgacga cgaacaaccg
      241 catggagctg acggcgatga tcgccgggct gcgcatgctc ggcccgggcg aggccggcga
      301 cgtctactcg gacagccggc tggtggtgca gacgctcacg cagtgggcgg cgggctggga
      361 gcgccgaggc tggaagcgca cgaccggcga ggtgaagaac ctcgagctgg tgcaggaggc
      421 gtacgcgctg gcgaaggcac ggccgcgggc gcgcatccag tggatccgtg cgcacgacgg
      481 ctcgcggtgg aacgagtacg ccgacgcact gtcgacggcg tatctgcgca gcgaggtgtg
      541 agggcagcga ggtccggggc gcctcgacgg cggtcaggcg gcgggcggcg cctgcgcgga
      601 tcggtgcacg aggtcacgca ggctgaggtc gagcgaccac atcgcgacga agccggtgac
      661 gccgatccag gcgagcagca gcaggtgcga gccgatggcg tagccggagg cggtgtcgcg
      721 cggggcgccc atggccacga gcgcctcggt gaccacgact tcgtaggggc cgacgttcca
      781 cggcgtcacg gggacggcga cggcgaggtt cgccgcgatg gtaacgacca gggcctccgc
      841 gacggtcatg ccgaggccga aggcgtggcc gatgaaccag tagacgccca cctcggtgac
      901 ccaggcgcag atcgagatgc cgatggcgac cgcggtggcg ctcgtcgtgc gcagcgaggc
      961 gaggccggcg acggtctcga cgaagcggcg gccgatgaac gggcgcgcgc gcgccgggac
     1021 gggccgcaac acgcggctgt gcgtcgggtc ccaggcggtg ccgtagcggg cggcgaggac
     1081 ggtgaccgcg aagatcgaca gcacgatcac cgccagcgtg agcagggtgg agcgcaacac
     1141 ctcggggagg tcgagcaggg cgatgcccgt cgtcagcagg atgacgaagg tcacgccatc
     1201 gaggagcgac tcgaccacga ggacggtgct cgccatctcg gcgcgggcga ccccgaagcg
     1261 gcggttgggc agctcgatgc ggaggaggtc accggcgcga aagggaatga ccgcgttcgc
     1321 gaggttcgag accaggaaga gcgcgaagag gtgccgcgtg gggatctcct cgtggtggcg
     1381 caggaagtag cgccagcggt aggcgtggat ccccttcgag gcggtgaaca tgagcaggcc
     1441 gggtaccagc cagccgtagc gcacgtccgg caactggcgg acggcgccga cgatgtcgac
     1501 gcgccagaac aacagcgcca gcgtgccgat gctgaggagc agctggagcg cgagcagcgt
     1561 gcggacggat cgaaagcgca cgggcctcct cgcggcaggg gtacggcttc accataccgt
     1621 gacgcggcag cgtggcgctc aggcgcggcg gatggcggcg gtgacgcggc cgacggcgac
     1681 gaggacgccc ttggcgtcac gcacgtcgac ctgggcccag gcgatggtgc ggcccgagcg
     1741 gagcacgcgg ccctcggcgg tgaggtccga ggtggcggcg tcgaggtagc tgatgttgag
     1801 gtcggtcgtg gacatgcctc gccaggcggg ctcgtcggcg gagcggatgg tgcggacggc
     1861 agtgccggcg gcggcgtcga tgagggtcgc gatggcgccg ccgtggacga cgtcggggcg
     1921 gttcacggtg ccgaactctg gacgcatcgc catcgcaacg acggcgcggc ccccctcggc
     1981 ctccacgaag cggatgccga ggtagcgcca gaacggcgtc tcctccggcg cgatccaggg
     2041 aagcagcgcg gggtcgatgt cggcgcccac ctcgctgggc cctagccgcc gaagagcttg
     2101 ccgagcgacc ccgtgagatc gtcgaggtcg acgggcgagt tgtcaccgag gaggctcgcg
     2161 agctgctcgg aattgccgcc caggaggccg aggagctgct gggggttctc ctggacgaag
     2221 ccgaggatgg tatcgacggc ctggcccgcc tggtcttcgt tcaggccgat gcgctcggcg
     2281 agcaggccga tgagctggtc tcgcatgcgg aacctcctgg ggcgcaggaa ggagcgtagc
     2341 acctcgaggg cggtcggaag gcgccgagtc gcgaccgcag tcgaggggcg tcgtagcatc
     2401 gagggtatgc aacgcgaggt catcgtcgtc ggggccgggc cggccgggag caccgcggcg
     2461 cgcgagatcg cgcggcgcgg gcacgaggta ctgctcgtcg accgggcgaa gttcccgcgg
     2521 gacaagccct gcggcggcgg ggtcacggtg cgctgtgcgg gcctgttgcc gttcgacatc
     2581 gagccggtgg tcgagcacgt cgtgaccggc gcaatcctgc aggggccgaa cggcaagacg
     2641 aaggtgcgcg agtcgggtgc accgctgacg ttcatgacgc agcgccgccg cctcgatgcg
     2701 ttcctcgcgg agcgggcgca ggaggcgggg gcggagttcc gtgacggcct cgcggtgcgg
     2761 gagttgaccg gcacggccga ggacttcgag gtgcgcctcg ccgacggcag cgaggtgcgg
     2821 gcgcgcgtgg tgatcggcgc ggacggcgcg aacggcgtcg tggggacgca cctggggttc
     2881 gaccacccgc tggagagcgc ggtcgccctc gaggggaacc tgccgtgccc ggacggtccg
     2941 ccgccgtggc tggccggcaa ggtcgcgctc tcgctcggct ggctgcccgg gggctatggc
     3001 tggctgtttc ccaagggcga ccacgtcaat gtgggcgtcg gtgggtggaa ggcggtcgtg
     3061 ggcgggcagc tgcgcgactc gctcgaggcg tacgcgcgct cgttcggctg ggaggcgtcg
     3121 atgctgacca acctgcgcgg ccaccacctc cccatgcagc ggccgggcac cgacgtggcg
     3181 ggcctcgcgg cggccgtgat cggggacgcg gcgggtctcg tcgacccgtt gtcgggggag
     3241 ggcatctacg cggcgattgc gtccggggtg gcggtggcgc cggcggccgt cgactacctc
     3301 gaggggcggg cgagcagcct gtcggggtac cagctggcgg tggaacggga actgcttccc
     3361 gacctggtcg catcgcgggc gctgatggag atcttccacg ccgcgcccgg gccgttctcg
     3421 tggctg
//
LOCUS       DAORPG010000141         3409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2499_length_3409_cov_3.610316, whole genome shotgun sequence.
ACCESSION   DAORPG010000141 DAORPG010000000
VERSION     DAORPG010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2499_length_3409_cov_3.610316"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /gene="glgP"
                     /locus_tag="PLX85_04230"
     CDS             <1..674
                     /gene="glgP"
                     /locus_tag="PLX85_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017292204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRC62412.1"
                     /translation="LLDEVANRTDQMLTPGTFTIGFARRAAAYKRADLVFTDLERLRR
                     ITDHVGPVQFIYSGKAHPQDVGGKDVIRRVFEAHRALRDEIPIVYLEDYDMELGGLVC
                     AGVDLWLNTPEQPLEASGTSGMKAALNGVPSLSVLDGWWIEGHVEGVTGWSIGHSPWE
                     GRSDPAAEADSLYTKLESSIVPAFYGARERYNEVMRSAIALNGSFFNTERMVRQYASS
                     AYTLR"
     gene            complement(680..1486)
                     /locus_tag="PLX85_04235"
     CDS             complement(680..1486)
                     /locus_tag="PLX85_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRC62413.1"
                     /translation="MADGLDLDALRDALEEAVRAAAAAILEVVARGDMRVGHKWGEGP
                     VTEADYAADETLHERLMPLIQGAQWLSEESEQVAPLLHGEPTWVVDPLDGTREFLRGL
                     PEYGVTVGLFLDDRLTLGAVALPGTEDVYSAIVTEERREARKNGQPLPALATDGEVRR
                     VVVSRHDYERRRLHYQIPFEVYPAGSAAVKLVHAADGSADVYLSTGPRSIWDVSGGAA
                     ILEGVGGTLLMPNGRPLPLSPRQVHVPPYAAGQREACLRVLRGIGQRVEL"
     gene            1538..3016
                     /locus_tag="PLX85_04240"
     CDS             1538..3016
                     /locus_tag="PLX85_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010581834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRC62414.1"
                     /translation="MRISLPFRQHATPRTPLVRALEAAVGSAHVLHLAEDLLAYEYDA
                     TIERRRPDAVVFPRSTEEVAACVRIANEHNVPIVPRGSGTGLAGGAVPAQGGLVVVLT
                     RMNRILELDPRDRIAVVQPGVINLALQEACAPHGLLYAPDPSSQRICTLGGNIANNSG
                     GPHTLAHGSTVNHVLGLEVVLPDGRVTWLGARQLDAPGVDLRGLFVGSEGTLGIVTQI
                     CVRLVRAPEAVRTMLAIFETIDEATEAVSAIIRRGIIPVALEMLDQEIIRAVEPQLHV
                     GYPLDAGAVLLIEVEGLRETVLEDEAAVIGVCRELHAREVRTAASEDERARLWEGRKG
                     GIGAIGAVSPNFYILDGVVPRTRLPEVMREVMRLSEAAGFRCANIFHAGDGNLHPNIM
                     FDELEPGAIGRVLDLGGQIMRVCVDAGGSITGEHGVGLEKRSYMEWIFGPPDLEAMER
                     VRVAFGGANRFNPCKLLPTGRGCGHAHATELLRHLETPGVYV"
     gene            3013..>3409
                     /locus_tag="PLX85_04245"
     CDS             3013..>3409
                     /locus_tag="PLX85_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRC62415.1"
                     /translation="MTASLGSDIESVLPMARQRPPAGYPIDGVTPRVAFRPETRDEVA
                     ALLQAADAAGFTVAPQGSRTTLRLGRPLAAYDVALDLTGLCRVVAYEPEDLTVTVEAG
                     MTLAALQRLLGERGQYLPVDPAPDDRVTVG"
BASE COUNT          520 a         1229 c         1167 g          493 t
ORIGIN      
        1 ggctcctgga cgaggtggcc aaccgcacgg atcagatgct cactcccggc accttcacga
       61 tcgggtttgc acggcgcgcg gcggcctaca agcgagcaga cctcgtgttc accgacctcg
      121 aacggctgcg ccgcatcacc gatcacgtcg ggccggtcca gttcatctac tcgggcaagg
      181 cgcatcccca ggacgtcgga gggaaggacg tcatccggcg ggttttcgag gcgcatcgag
      241 cgctgcgcga cgagatcccg atcgtctacc tcgaggacta cgacatggag ctgggcgggc
      301 tcgtctgcgc gggcgtcgac ctctggctca acacaccaga gcagcccctc gaggcgtccg
      361 gtaccagcgg catgaaggcc gcgctcaacg gcgtgccaag cctcagcgtc ctcgacggct
      421 ggtggattga agggcacgtc gagggcgtca ccgggtggtc gatcgggcac agcccgtggg
      481 agggccgctc ggaccccgcc gccgaggccg actcgctcta cacgaagctc gagtcgagca
      541 tcgtgccggc gttctacggc gcgcgcgaac gctacaacga ggtcatgcgc tcagcgatcg
      601 cactcaacgg ctcgttcttc aacaccgagc gcatggtgcg gcagtacgcc tcgagcgcgt
      661 acaccctccg ctgacccgcc tacagctcca ctcgctggcc gatgccgcgc aacacccgca
      721 ggcacgcctc acgctgcccc gcggcatacg gcggcacgtg cacctggcga ggtgagagcg
      781 gaagcggcct cccgttcggc atgagcaacg tgccccccac gccctcgagg atggccgccc
      841 cgccggagac gtcccagatc gaccgcggcc ccgtggagag gtagacgtcc gccgatccgt
      901 ccgcagcatg cacgagcttc accgccgccg atccggccgg atacacctcg aacgggatct
      961 ggtagtgcag acgtcggcgc tcgtagtcat gccgcgagac gacgacgcga cggacctcgc
     1021 cgtcggtcgc cagcgccggc agcggctgcc cgttcttgcg ggcctctcgg cgctcctcgg
     1081 tcacgatggc cgaatacaca tcttccgtcc cgggaagcgc gacggccccg agggtcagcc
     1141 ggtcgtcgag gaacaggcct accgtcactc cgtactcggg aagcccccgc aggaactctc
     1201 gcgtcccgtc cagcgggtcg acgacccagg tgggctcgcc gtggagcaac ggcgccacct
     1261 gctcggactc ctcggacagc cactgtgccc cctggatgag gggcatcagc cgctcgtgca
     1321 atgtctcgtc ggcggcgtag tcggcctcag tgacggggcc ctcgccccac ttgtggccga
     1381 cgcgcatgtc accgcgcgcg accacctcga ggatcgccgc ggctgcggca cgcaccgcct
     1441 cctcgagcgc atcgcgcagg gcgtcgaggt cgagtccgtc cgccaacgtg tcccctttcg
     1501 agggctcccg gaggccgatg ctacactccg gggcgaaatg cgcatctcgc tcccgttccg
     1561 ccagcacgcg acgccacgca cgccgctcgt gcgagcgctt gaggccgcag tgggctcggc
     1621 gcacgtgctg catctggcgg aagacctgct ggcgtacgag tacgacgcca ccatcgagcg
     1681 acggcgaccg gacgccgtcg tcttcccgcg gagcaccgag gaggtcgccg cctgcgtccg
     1741 catcgcgaac gagcacaacg tgccgatcgt cccgcgcggc tcgggcaccg gtctcgccgg
     1801 cggcgctgtg ccagcacagg gcggcctcgt ggtggtgttg acgaggatga accgcatcct
     1861 cgagctggac ccgcgcgacc gcatcgcggt ggtgcagccc ggcgtcatca acctcgccct
     1921 gcaagaggcg tgcgcgcccc acggactgct ctacgcgccc gacccgtcct cgcagcgcat
     1981 ctgcacgctc ggcggcaaca tcgcgaacaa ctcgggcggc ccgcacacgc tcgcgcacgg
     2041 tagcacggtc aaccacgtgc tgggcctcga ggtcgtgctg cccgatgggc gcgtcacgtg
     2101 gctcggtgcc cgccagctcg acgccccggg agtggacctg cgtggcctct tcgtgggctc
     2161 cgagggcacc ctgggaatcg tgacgcagat ctgcgtccgt ctcgtgcgcg cgccggaagc
     2221 ggtgcgcacg atgctcgcca tcttcgagac gatcgacgaa gccacggaag cggtgtcggc
     2281 gatcatccgg cgcggcatca tcccggtcgc gctcgagatg ctcgaccagg agatcatccg
     2341 cgccgttgaa ccgcagctcc acgtcggcta cccgctcgat gcgggcgccg tgctgctgat
     2401 cgaggtggaa gggctgcgcg agacagtcct cgaggacgag gcagcggtga tcggcgtctg
     2461 tcgcgaactc catgcccgcg aggtgcgcac ggcggcttcg gaggacgagc gggcgcgact
     2521 ctgggaaggc cgtaagggcg gcatcggcgc gatcggggcg gtcagcccga acttctacat
     2581 cctcgacggc gtcgtgcctc gcacgcggct gccagaagtg atgcgcgagg tcatgcggct
     2641 gtccgaggcg gccggctttc gctgcgcgaa catcttccac gcgggcgatg gcaacctgca
     2701 cccgaacatc atgttcgacg aactggagcc cggcgcgatc ggtcgcgtcc tcgacctggg
     2761 cggccagatc atgcgcgtgt gcgtggacgc cggcggatcg atcaccgggg agcacggcgt
     2821 cggcctcgaa aagcggtcgt acatggagtg gatcttcggc ccccccgacc tcgaggcgat
     2881 ggagcgcgtc cgggtcgcct tcggcggcgc caaccgcttc aacccgtgca agctgctgcc
     2941 cactggccgg ggttgcgggc atgcccatgc caccgagctg ctccggcacc tcgagacgcc
     3001 gggggtgtac gtgtgaccgc cagcctcggc agcgacatcg agtcggtcct ccccatggcg
     3061 cgacaacggc cgccggccgg ctacccgatc gacggcgtca cgcctcgtgt cgccttccgc
     3121 ccggagactc gtgacgaggt ggcagcgctg ctgcaggcag ccgacgcggc cgggttcacg
     3181 gtcgcgccgc agggcagccg cacaaccctc cgactcggcc ggccgctggc ggcgtatgac
     3241 gtcgcgctcg acttgaccgg actgtgccgc gtggtggcgt atgagcccga ggatctgacc
     3301 gtgacggtgg aggcgggcat gaccctcgcc gcgctgcagc gcctgctggg cgagcgaggc
     3361 cagtacctgc cggtcgaccc ggcgcccgat gaccgcgtga cggtcggcg
//
LOCUS       DAORPG010000142         3398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2520_length_3398_cov_3.695782, whole genome shotgun sequence.
ACCESSION   DAORPG010000142 DAORPG010000000
VERSION     DAORPG010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2520_length_3398_cov_3.695782"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PLX85_04250"
     CDS             complement(<1..463)
                     /locus_tag="PLX85_04250"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC62416.1"
                     /translation="MDWNDSPEQAAFRADAAEFIRSRLPEYYRDSDEVEEEGLFGWYA
                     DRISEDPNARKAAADWASALAERGWVAPHWPKEYGGGGLTTMEQFVLGAEMAKAHAPM
                     VGGSGVLLLGPTLIIHGSDDLKQRVLPKILKGETAYAQGYSEPGAGSDLGSL"
     gene            complement(561..803)
                     /locus_tag="PLX85_04255"
     CDS             complement(561..803)
                     /locus_tag="PLX85_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62417.1"
                     /translation="MILLPLLVAIAMYLAWRLWLKLHGVKWKPQGRWVPRTAAAEEYW
                     STLPQPSTGQKIMLGIIGLVVVAAEVVFLVWAVRRN"
     gene            complement(833..1837)
                     /locus_tag="PLX85_04260"
     CDS             complement(833..1837)
                     /locus_tag="PLX85_04260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRC62418.1"
                     /translation="MRLGIVFQEDYSGPGALDRQIEFVRAADELGYELICSTETWGLS
                     ALPWLTVMARETRNIRLGTAIINHFSRSPAAIAQEFAALDVISGGRAVLGLGSSASAV
                     IEGFHGVPFDRPLLRLKEYVEIFKTLIRGDRLHYDGALFHLDRGFSLDYARPRTDIPV
                     YIASITPRSIRQTAQVADGIIPIHWPKQRFSDLRRELDDAARAAGRDPSALTIAPHMS
                     VWITDGANDEAQWHAAREHLSHYINRMGDFYWQMFERQGYEAEVAASRAAFAQRDREG
                     AIAAISDRMVRDIDVIGPVESAREQLQERAELGVDIPLMKKLPTDPKLARPILEALIR
                     "
     gene            complement(1900..2841)
                     /locus_tag="PLX85_04265"
     CDS             complement(1900..2841)
                     /locus_tag="PLX85_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC62419.1"
                     /translation="MTMDQTELMAAAARAQNIAVPAVVAPTDHWVKLNGINFHYLDWG
                     NEHLPHVVLLHGGSLTAHTWDMAALLLRDRYHLVCLDQRGHGDTDWTPDDRLSEDNND
                     LMLEDTRQFIEYLNYDHMTLVGMSMGGMNTIRYAARHPERLDAVGIVDIAPETMREGQ
                     LEMEAFRSATETLNTFDDFLNRAIQFMPHRPPDHLRYSLTHSLKPTAEGWTWKQDHRP
                     RPGMQDLTEEQRKEENARRADELWADIRAIHQPTILFKGADSKILAQDVAERTVAAMT
                     NARLVVIPRATHNVHSDNPVDFARALDAFLTEVLPKR"
     gene            3059..>3398
                     /locus_tag="PLX85_04270"
     CDS             3059..>3398
                     /locus_tag="PLX85_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62420.1"
                     /translation="MPVQPLAEWQQRWENEGAANLKVRPVEVAEGYAQFVIDLPYGDE
                     RDGDLDFLNLALSYAVDVAALAAVIARVDPEREQPNGTASLHLNFLEAPSEPVTVEGR
                     VVYWQVPSALI"
BASE COUNT          473 a          961 c         1399 g          565 t
ORIGIN      
        1 gcagcgagcc gaggtcagag ccggcgcccg gctcggagta gccctgggcg tatgcggtct
       61 cgcccttgag gatcttgggg aggacgcgct gcttgaggtc gtcgctgccg tggatgatga
      121 gggtggggcc gaggaggagg acgccggagc cgccgaccat gggggcgtgc gccttcgcca
      181 tctccgcgcc gaggacgaac tgctccatcg tggtcaggcc gccgccgccg tactccttgg
      241 gccagtgggg ggcgacccag ccgcgctcgg cgagggcgga ggcccagtcg gccgcggcct
      301 tgcgggcgtt gggatcctcc gagatgcggt cggcgtacca gccgaagagg ccttcctcct
      361 cgacctcgtc ggagtcgcgg tagtactcgg gcaggcggct gcggatgaac tcggcggcgt
      421 cggcgcggaa ggcggcctgt tcgggtgagt cgttccagtc catggggtgc tggtctcctg
      481 attctcgtgt ggtcgattcg ggagcgatta tccggcgcgc ctcgggaggg ggtcaacgaa
      541 cgcggggcgg aggtcgaggg tcagttgcgc cgcaccgccc ataccaggaa gacgacttcg
      601 gcagccacga ctacgaggcc gatgatgccg agcatgatct tctgcccggt actcggttgc
      661 ggcagagtcg accagtactc ctcggcagcg gcggtccgcg gcacccaccg accctgcggc
      721 ttccacttga ctccgtgcag cttgagccag agccgccagg ccaggtacat ggcgatggcg
      781 accaagagcg gcagcaagat catcaggtag ccctgtggct gttccctctc cgctagcgga
      841 tcagcgcctc gaggatcggg cgggcgagct tcgggtcggt cgggagcttc ttcatcaggg
      901 ggatgtcgac gccgagttcg gcgcgctcct ggagttgctc gcgggcggac tcgacggggc
      961 cgatgacgtc gatgtcgcgg accatgcggt ctgagatggc cgcgatggcg ccctcgcggt
     1021 cgcgctgggc gaaggcggcg cgggaagcgg cgacctcggc ctcgtagccc tgccgctcaa
     1081 acatctgcca gtagaagtcc cccatgcggt tgatgtagtg cgacaggtgc tcgcgtgcag
     1141 cgtgccattg cgcctcgtcg tttgcgccgt cggtgatcca gacggacatg tggggcgcga
     1201 tggtgagcgc cgaggggtcg cggccggcgg cgcgtgcggc gtcgtcgagc tcacggcgga
     1261 ggtcggagaa gcgctgcttc ggccagtgga tggggatgat gccgtctgcg acctgggcgg
     1321 tctggcggat cgagcgcgga gtgatcgagg cgatgtagac ggggatatcc gtgcgcgggc
     1381 gggcgtagtc gaggctgaag ccgcggtcga ggtggaagag ggcgccgtcg tagtggaggc
     1441 ggtcacctcg gatcagggtc ttgaagatct cgacgtactc cttgaggcgg agcaggggac
     1501 ggtcgaaggg gacgccgtgg aagccctcga tgacggcgga ggcggaggag ccgaggccga
     1561 ggacggcacg gccgccggag atgacgtcga gggcggcgaa ctcctgcgcg atggcggcgg
     1621 ggctacgcga gaagtggttg atgatggcgg tgccgaggcg gatgttgcgc gtctcgcgcg
     1681 ccatgacggt gagccagggg agggcggaga gcccccaggt ctcggtgctg cagatcagtt
     1741 cgtagccgag ttcgtcggcg gcgcggacga actcgatctg ccggtcgagc gcgccggggc
     1801 cggagtagtc ctcctggaac acaatgccga gccgcatggc cgcctcctgt gacgcctcga
     1861 gggtggttgg tggaagcgat ggtacggcgc cggggggcct tatcgcttcg gcaggacctc
     1921 cgtgaggaag gcgtcgaggg cgcgggcgaa gtcgacgggg ttgtcggagt ggacgttgtg
     1981 ggtggcgcgg gggatgacga cgaggcgggc gttggtcatc gcggcgacgg tccgttccgc
     2041 gacgtcctgg gcgaggatct tgctgtcggc gcccttgaac aggatggtcg gctggtggat
     2101 ggcgcggatg tccgcccaga gctcgtcggc gcggcgggcg ttctcctcct tgcgctgttc
     2161 ctcggtcagg tcctgcatgc ctgggcgggg acggtggtcc tgcttccagg tccagccctc
     2221 agcggtcggc ttcaggctgt gggtgaggct gtagcggagg tggtcgggcg ggcggtgggg
     2281 catgaactgg atcgcgcggt tcaggaagtc gtcgaaggtg ttgagcgtct cggtcgcgct
     2341 gcggaaggcc tccatctcga gctggccctc acgcatggtc tcgggggcga tgtcgacgat
     2401 gccgacggcg tcgaggcgct ccgggtggcg ggcggcgtag cggatggtgt tcatgccgcc
     2461 catcgacatg ccgacgaggg tcatatggtc gtagttcagg tactcgatga actggcgggt
     2521 gtcctcgagc atgaggtcgt tgttgtcctc ggagagccgg tcgtcggggg tccagtcggt
     2581 gtcgccgtgg cctcgctggt cgaggcagac gaggtggtag cggtcgcgga ggaggagggc
     2641 ggccatgtcc caggtgtggg cggtgaggga gccgccgtgg agcagcacga cgtggggcag
     2701 gtgctcgttg ccccagtcca ggtaatggaa gttgatgccg ttgagtttga cccagtggtc
     2761 ggtgggggcg acgacggcgg ggacggcgat gttctgggca cgggcggcgg ccgccatcag
     2821 ctcggtctgg tccatggtca tcgctgcgct cctcctcgat ggctgcgcgt cggcgctggg
     2881 gggtcggtgg cgggcatcgt acacgagggt ggcgcaagcg acgtgagagg tggctgccga
     2941 gcggggggag ggggccggac ctccggactg ggacgtcgag cagaggcggc gcggccgcgc
     3001 ctcgacggtg ggcggagggc gctggtatcg tgccggacgc atcgagggag ggtgacggat
     3061 gccggtgcag ccactggcgg aatggcagca gcgctgggag aacgaggggg cggcgaacct
     3121 caaggtgcgg ccggtggagg tcgccgaggg gtacgcgcag ttcgtcatcg acctgccgta
     3181 cggggacgag cgtgacggtg acctcgactt cctgaacctg gcgctgtcgt acgcggtgga
     3241 cgtcgcggcg ctggcggcgg tgatcgcgcg cgtcgacccg gagcgcgagc agccgaacgg
     3301 tacggcctcg ctgcacctga acttcctcga agcgccgtcc gagccggtga ccgtcgaggg
     3361 gcgggtcgtg tactggcagg tgccgagcgc gctgatcg
//
LOCUS       DAORPG010000143         3374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2551_length_3374_cov_3.291353, whole genome shotgun sequence.
ACCESSION   DAORPG010000143 DAORPG010000000
VERSION     DAORPG010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2551_length_3374_cov_3.291353"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PLX85_04275"
     CDS             <1..387
                     /locus_tag="PLX85_04275"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HRC62421.1"
                     /translation="AAVIALFALGAIVTGAVILNSGGGSEELVRNVASGPAAGSTLRY
                     DPGNDRAILDVAGLAALPQGRTYQVWMLRGDSVAGVGLFTVDPSGRGRAVFTAEMQTG
                     DTVAVTEEPAGGSPAPTSQPLFAIQL"
     gene            496..2010
                     /locus_tag="PLX85_04280"
     CDS             496..2010
                     /locus_tag="PLX85_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62422.1"
                     /translation="MAAARRTVPTTAKPAPRPRRSGPVKVTPNLPAIPDLGGILDGVT
                     KRGSLDLTLVIVPHLGDLEAARARAAEYARSYAEVSGVEQPEPLADLAGIQARVADAI
                     RSGIAEVLGRAPFVSIVDEGSAVDLPDPPKVTAENLSTVLTPESYLEALLAVVLLNNT
                     RRTRIGISTGTDGPPRDRDVLAADVISMTGPELSEDGSKFAAGFGVVVRPSFVLALAN
                     TLLTPLSLDGNPCECGPIVLSNPRVTMADGRMTDRVDATVANAITATVTLVDQVRVLH
                     GFQSSSITGRVDSTDVAVKPITARAKKLEAEAWRNAFLAMVAPPHLGRMAVRGILGRV
                     AEHYMPALPSPIAMLGAALPFGPRPLAPSVDAQGNPIPSAQTLVLGARHLDGTTALKA
                     DITSALRVSGLVQLRPRQASVAINVQVRDSAHREFSVTASDVEMIDPSFTWTITGATV
                     ETSLEAAEVHFRAAQHAVVQLHVTAAGTDGLTTEADLTLDVDALLKDLTATPTT"
     gene            2216..>3374
                     /locus_tag="PLX85_04285"
     CDS             2216..>3374
                     /locus_tag="PLX85_04285"
                     /inference="COORDINATES: protein motif:HMM:NF025585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HRC62423.1"
                     /translation="MHVRTRRWSSLGGSALAVIGIALLAFALFGLRAEGADHKDSPSV
                     EGDAGADITDVYAFRSPTNPNNLVVALDVNPLTPPSANANTNFASGVDYRIHVDNNGD
                     LNADATVVVKFNGNDFTVSGLGNTPITGKVTPAGTAPMVTDAGGIKVFAGLRDDPFFF
                     DLTGFKAFVAGPYTPANGLRQSGQTPADTFAGTNVSAIVIELPITALTGAANANTGTI
                     KAWVSTARGSNQVDRMAIPAINTALIPSTSKNAFNQASPSGDVATFRPMAQASVTGLR
                     DAVKSVLPAEDGGPLGKLTPEQVAGALIPDVVTIDFSKDVVFPNGRRLQDDVIDAALG
                     IVLNRGGAKGVGDAVNANDKAFLGTFPYLAEPFMAAAQASPTATAGASGTTM"
BASE COUNT          537 a         1306 c         1014 g          517 t
ORIGIN      
        1 gctgccgtta tcgctctgtt cgcgctcggc gcgatcgtca ccggcgccgt catcctgaac
       61 tcgggcggtg gctccgagga gctcgtccgc aacgtggcca gcggtcccgc cgctggttcg
      121 acgctccgct acgaccccgg caacgaccgc gccatcctcg acgtcgcggg actcgccgcc
      181 ctccctcagg gccgcacgta ccaggtgtgg atgctgcgag gcgactcggt tgccggcgtc
      241 ggcctcttca cggtggaccc ctcggggcgc ggtcgtgccg tcttcaccgc ggagatgcag
      301 acgggggaca ctgtcgccgt caccgaggaa cccgccggcg gctcgcctgc cccgacctct
      361 cagccgctgt tcgcgatcca gctctgaccc gagatccgtt cgcgtccgct cctcgtaaaa
      421 cactacgatg gcttcgccag ttcccggctc gcgcgtcgag accggtgccc aggcgttgag
      481 aaaggatcgc gctcgatggc cgctgcacga cggaccgtcc cgaccacggc gaaaccggcg
      541 cctcgtcccc ggcgctcggg accggtcaag gtcactccga acctgccggc catccccgac
      601 ctcggcggca tcctcgacgg cgtcacgaaa cgaggctcac ttgacctcac actcgtcatc
      661 gtcccgcacc tcggcgacct cgaagccgcg cgggcccggg ccgccgagta cgcacgcagc
      721 tatgctgagg taagcggtgt tgagcagccc gagccgctcg cggacctcgc gggcatccag
      781 gcccgcgtcg ccgacgccat ccgatcgggc atagccgaag tcctcggccg ggcccccttc
      841 gtttcgatcg ttgacgaggg aagcgccgtc gatcttcccg acccgccgaa ggtgaccgcc
      901 gagaacctga gcaccgtgct caccccggag tcgtacctcg aggccctgct cgcggtcgtg
      961 ctgctgaaca acacccgccg tactcggatc gggatttcga cgggcacgga cggtccgccg
     1021 cgcgaccgtg acgtgctcgc cgccgacgtc atctctatga ccggccctga gttgagtgag
     1081 gacggatcca aattcgcggc cggctttggt gtcgtcgtgc gtccgtcgtt cgtgctcgca
     1141 ctggcgaaca cgctgctcac ccccctgtcg ctcgacggca atccctgcga gtgcggcccc
     1201 atcgtgctct cgaacccgcg cgtcacgatg gcggacggcc gcatgaccga ccgtgtcgac
     1261 gccaccgtcg ccaacgccat caccgcgacc gtcaccctgg tcgatcaggt gcgcgttctg
     1321 cacggcttcc agtcgagctc gatcaccggc cgcgtggatt cgacggacgt cgccgtgaag
     1381 ccaatcaccg cccgcgcaaa gaagctcgag gccgaggcgt ggcggaacgc cttcctcgcg
     1441 atggtcgccc cgcctcatct cgggcgcatg gccgtccgtg gcatccttgg ccgagttgct
     1501 gagcactaca tgcccgcgct tccgagtcca atcgcgatgc ttggagcggc gctgccgttc
     1561 ggcccgcgcc cgctcgcccc gtccgtcgac gcccagggca acccgatccc ctcggcccag
     1621 acgctggtcc tcggcgcgcg ccacctggat ggcacgacgg cgctgaaggc cgatatcacc
     1681 agcgccctcc gggtgtctgg cctcgtccag ctccgtccga ggcaggcgtc cgtcgccatc
     1741 aacgtccagg tgcgcgactc cgcacatcgc gagttctccg tcaccgcgag cgatgtcgag
     1801 atgattgacc cgtccttcac ctggaccatc acgggcgcga ccgtcgagac gagcctcgag
     1861 gcggctgagg tccacttccg cgctgctcag catgccgtcg tccaactgca cgtgaccgcc
     1921 gccggtacgg acggcctgac caccgaggca gacctgaccc tggacgtcga tgccttgttg
     1981 aaggacctca cggccacgcc aacgacctga ctcgggggca gacctcaccc attcggcgcc
     2041 ctgcccgggc aggggacgga ctcagccgtg tcagaaacgt aacaactcgc cgaaactctg
     2101 gtccgaagtc cctccacgca ctgatccctt ccgcgaatcc ccgcgaacta agggcatgcg
     2161 accggctgaa cccgggagcc ggccgcatga ccagttcgcc gcgaaaggag catcagtgca
     2221 cgtacgaacc agaaggtggt catccctcgg aggctcggcg cttgccgtca tcggcatcgc
     2281 gctccttgcc ttcgcgctct tcggcctccg cgccgagggt gccgaccaca aggactcgcc
     2341 gagcgtcgag ggggacgctg gcgccgacat caccgacgtc tatgcattcc ggagcccgac
     2401 gaaccccaac aacctcgtgg tggccctgga cgtcaacccg ctcaccccgc cgtccgcgaa
     2461 cgcgaacacc aacttcgcga gcggcgtcga ctaccgcatc cacgtcgaca acaacggcga
     2521 cctcaacgcg gacgccaccg tcgtcgtcaa gttcaacggc aacgacttca ccgtctccgg
     2581 cctcggcaac acgccgatca ccggcaaggt gacgcccgcc ggaaccgcgc caatggtcac
     2641 agacgccggc ggcatcaagg tcttcgccgg cctgcgtgat gacccgttct tcttcgacct
     2701 gaccgggttc aaggccttcg tcgctggccc ctacacgccc gcgaacggcc tccggcagtc
     2761 gggccagacg cccgccgaca ccttcgccgg caccaacgtc tcggccatcg tcatcgagct
     2821 cccgatcacg gccctcaccg gcgcggccaa cgccaatacc ggaaccatca aggcctgggt
     2881 cagcaccgct cgcggctcca accaggtcga ccgcatggcg atcccggcca tcaacacggc
     2941 cctgatcccc agcacctcga agaacgcgtt caaccaggcc agcccgtctg gcgacgtcgc
     3001 caccttccgc ccgatggccc aggcctcggt caccggtctg cgtgacgccg tgaagtccgt
     3061 cctccccgcc gaggatggcg gtccgctcgg caagctcacc cctgagcagg tcgccggcgc
     3121 cctcatcccc gacgtcgtga ccatcgactt ctcgaaggac gtggtcttcc cgaacggccg
     3181 tcggctccag gatgacgtca tcgacgccgc cctcggcatc gtcctgaacc gcggtggcgc
     3241 gaagggtgtc ggcgacgccg tcaacgccaa cgacaaggcc ttcctgggga ccttccccta
     3301 cctcgccgag ccgttcatgg ccgccgccca ggcgagcccg acggcgaccg cgggcgcctc
     3361 tggcaccacg atgc
//
LOCUS       DAORPG010000144         3359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2585_length_3359_cov_3.110775, whole genome shotgun sequence.
ACCESSION   DAORPG010000144 DAORPG010000000
VERSION     DAORPG010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2585_length_3359_cov_3.110775"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PLX85_04290"
     CDS             complement(<1..451)
                     /locus_tag="PLX85_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRC62424.1"
                     /translation="MRLYNTLTGAIEELTPVTPGRLGIYACGVTPYSESHVGHAMSAI
                     VYDVFVRYLRWRGNPAGGYEVTFVSNYTDVDDKLIDRGRELGIDPLELGERNIALWEE
                     QQRALNLTFPDIRPRVTTHIEPIVALTERLIETGHAYATPSGDVYFRV"
     gene            complement(453..1139)
                     /gene="cysE"
                     /locus_tag="PLX85_04295"
     CDS             complement(453..1139)
                     /gene="cysE"
                     /locus_tag="PLX85_04295"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018998714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HRC62425.1"
                     /translation="MGWLAHLREDISVARQRDPAARSSFEILLTYPGIHALFIHRIAH
                     GLAVRGWVVLPRLIAHLGRALTGIEIHPRAEIGRRLFIDHGMGIVIGETAVIGDDCHL
                     YQGVTLGGTSTRREKRHPTLEDHVTVGAGAIIIGAVTLGTHARIGAGSVVVSNVPPHA
                     TVVGVPGHIVAITNATNDTVERLPDPEWDRLQDLERRVTRLEAGALAPAPTGGGFDGG
                     PPATRPEERA"
     gene            complement(1265..1744)
                     /gene="ispF"
                     /locus_tag="PLX85_04300"
     CDS             complement(1265..1744)
                     /gene="ispF"
                     /locus_tag="PLX85_04300"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRC62426.1"
                     /translation="MRIGIGYDIHRFRESGTLRLGGIEVPDAPQLDGHSDGDGLIHAI
                     IDALLGAAGEGDIGRHFPPGDPSTEGIDSRELLTRVVRLVVSRGYRVHNIDATVVAER
                     PRLATHLPAMRDSLARCLGVPASSVNVKATTNERLDALGTGEALAAMAVALLEEEVP"
     gene            complement(1880..2170)
                     /locus_tag="PLX85_04305"
     CDS             complement(1880..2170)
                     /locus_tag="PLX85_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62427.1"
                     /translation="MPHRRVPRPFNLVWGSGRVIEEAAIEGGLHAPSLQLLAYDDGEK
                     KGQLAVRFAAYDPEGELERRPLIINERELAALRRELDHTPRMKALLKRLLDE"
     gene            2340..3158
                     /locus_tag="PLX85_04310"
     CDS             2340..3158
                     /locus_tag="PLX85_04310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC62428.1"
                     /translation="MRPEEAALAVEWAALEGWNPGLHDAEAFLATDPDGFFVGELDGE
                     VISSLAGVAYDDSYGFLGLYIVKPEQRGKGYGLRLWQEVTAYLADRNVGLDGVLAQQA
                     NYERSGFTLAYRNVRYEGHRPAVTRDAAGIVELSSVPFDDVLAYDRSVFPVERRGFIQ
                     RWIAMPGSVALGLRRGAAFAGFGVARKCRAGYKIGPLSADDETAADDLFRALCARLDD
                     DLVYLDVPEPHREAVALAERYGMQPSFETVRMYNQREPAVDLGRIFGVTTFELG"
BASE COUNT          494 a         1054 c         1276 g          535 t
ORIGIN      
        1 gaacgcggaa atagacgtcg cccgacggcg tcgcgtaggc gtggccggtc tcgatgaggc
       61 gctcggtgag cgcgacgatc ggctcgatgt gcgtcgtgac ccgggggcga atgtcgggga
      121 atgtcaggtt cagcgcgcgc tgttgctcct cccagagcgc gatgttccgc tcccccagct
      181 cgagcgggtc gatgccgagc tcgcgaccgc ggtcgatgag cttgtcatcg acgtcggtgt
      241 agttggagac gaaagtgact tcgtagccgc cggcggggtt gccgcgccag cggagatagc
      301 gcacgaacac gtcgtagacg atggcgctca tcgcgtggcc gacgtggctc tcggagtacg
      361 gcgtgacgcc gcaggcgtag atgccgaggc gccccggcgt aacgggcgtc agctcctcga
      421 tggcgccggt gagtgtgttg tacagccgca tttcaggctc gctcctcggg tcgagtcgca
      481 gggggaccgc cgtcgaagcc accgcccgtg ggggcgggtg cgagtgcacc ggcctcgagg
      541 cgcgtcacgc ggcgctcgag gtcctggaga cggtcccact cggggtcggg caggcgctcg
      601 acggtgtcgt tggtggcatt ggtgatcgcg acgatgtgac cgggcacccc gacgacggtc
      661 gcgtgcggcg ggacgttgga gacgaccacg ctgccggcgc cgatgcgcgc gtgtgtgccg
      721 agcgtgaccg caccgatgat gatcgcgccc gcgccgacgg tcacatggtc ctcgagggtc
      781 gggtggcgct tctcgcggcg cgtcgaggtg ccgccgagcg tgacgccctg gtagaggtgg
      841 cagtcgtcgc cgatgaccgc cgtctcaccg atcacgatgc ccatgccatg gtcgatgaag
      901 agccgccggc cgatctccgc gcgcgggtgg atctcgatgc cggtcagcgc gcggccgagg
      961 tgcgcgatca gcctcggcag gacgacccag ccgcggacag cgaggccgtg cgcgatgcga
     1021 tggatgaaga gggcgtggat gcccgggtag gtgaggagga tctcgaagga ggaacgggcg
     1081 gccgggtcac gctgccgagc gacgctgatg tcttccctga ggtgtgcgag ccagcccatc
     1141 ggcggcccct ttctgcgcgt ggtgaattcg ggcggcggct gcggggcgcg tcccggaccc
     1201 ggggcgcccc gaccgacacg cgcaggtgtc cggcgagggg agatcgaacg cgaagttcat
     1261 cgcgttatgg tacctcctct tcgagcaagg cgacggccat cgccgcgagc gcctcgcccg
     1321 tgccgagcgc gtcgaggcgc tcgttcgtgg tggccttcac gttgaccgac gacgccggca
     1381 cgccgaggca gcgcgccaac gagtcgcgca tcgcgggcag gtgcgtggcg agcctcggac
     1441 gttccgcgac cacggtcgcg tcaatgttgt gcacccgata gccgcgcgag acgacgaggc
     1501 gcacgacacg cgtgagcagc tcgcgactgt cgatgccctc ggtcgagggg tcgcccggag
     1561 ggaagtggcg gccgatgtcg ccctcacccg cggcgccgag gagcgcgtcg atgatggcgt
     1621 ggatcaggcc gtcgccgtcg ctgtggccgt caagctgtgg ggcgtcgggc acctcgatgc
     1681 cgccgaggcg cagcgtgccg ctttcgcgga agcggtggat atcgtagccg atgccgatgc
     1741 gcacgctggc tgccttctcc tcgacgcccg tgcattgtgt ggggcagagg cgcaaggcgt
     1801 cgagggcggc tcatacgggc gactccacgc ccgggtcgcg tcgagacgcg tcgtctcagt
     1861 gcccggcccg gaggctggct tactcgtcga ggaggcgctt gaggagggcc ttcatgcgag
     1921 gggtgtggtc gagctcgcgg cgaagggcag caagttcgcg ctcgttgata atcagggggc
     1981 ggcgctccag ctcaccctca ggatcgtagg cggcgaagcg gacggcgagc tggcccttct
     2041 tctcaccgtc gtcgtaggcg aggagctgca acgagggagc gtgtaggcca ccctcgatgg
     2101 cggcctcctc gatcacgcga ccgctgcccc agacgaggtt gaacgggcgg ggaacgcgtc
     2161 ggtgtggcat cggggaacct ccttcaccac tggccggtgg gcgtacatat cacagcgatg
     2221 attctgactt gtccagcaac cgcgcgcgtc gcgggactcg cggacgcgtt aggctactgc
     2281 ctcgagacgg ccgcagcgcg aggaggagta cgggagtgag cgagtacgtg gttcggagga
     2341 tgcggccgga ggaggcggcg ctcgcggtcg agtgggccgc cctggaaggc tggaacccgg
     2401 gactgcacga cgcggaggca ttcctcgcga ccgatccgga tgggttcttt gtgggggagc
     2461 tggatggcga ggtgatctcg agcctcgcgg gcgtcgcata cgacgactcg tacggattcc
     2521 tcggcctgta cattgtgaag cccgagcagc gcggcaaggg gtacgggctc cggctctggc
     2581 aggaggtcac ggcgtacctc gcggaccgga acgtgggact cgacggcgtg ctggctcagc
     2641 aggcgaacta cgagcgatca gggttcacgc tggcgtaccg aaacgtgcgg tacgagggtc
     2701 accgcccagc ggtcacgcga gatgcggcgg gaatcgtcga gctgtcttcg gtgccgttcg
     2761 acgacgtgct ggcgtacgac cggagtgtgt ttcccgtgga gcgccgcggg ttcatccagc
     2821 gatggattgc gatgccgggc agtgtcgcgc tgggactgcg gcgcggggct gccttcgctg
     2881 gcttcggcgt tgcgaggaag tgccgcgcgg gctacaagat cggccctctc tcagcggacg
     2941 acgagactgc ggcggacgac ctgtttcgcg cgctctgtgc gcgactcgat gacgacctgg
     3001 tgtacctcga cgtgcccgaa ccgcatcgcg aggcggtcgc gctggccgag cgatacggca
     3061 tgcagccatc gttcgagacc gtgcgcatgt acaaccagcg agagcccgcg gtggacctcg
     3121 gccggatctt cggcgtcacg accttcgaac tggggtagac caccggcgac cgtgcctcag
     3181 cagcgcatac tgatcgggca tgcgggggca cattctcggt ctcggttgct tcgttgttgc
     3241 cgtgctggtc gcggttgtgc ccctcgatgg ccgccacgcc cagccctccg cggcggcgag
     3301 cgaccctcca cccgccaccc tgaccaccgg gaccgacgcg gcggcggccg tcgcggagc
//
LOCUS       DAORPG010000145         3352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2596_length_3352_cov_3.696997, whole genome shotgun sequence.
ACCESSION   DAORPG010000145 DAORPG010000000
VERSION     DAORPG010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2596_length_3352_cov_3.696997"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1736
                     /locus_tag="PLX85_04315"
     CDS             135..1736
                     /locus_tag="PLX85_04315"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HRC62429.1"
                     /translation="MPRLAAPVAVLALALLALSVLPAEAAKNRPLHLQLLGINDLRGE
                     LDPSTLDGRPVGGAAVLGTYLAQAVHDADAEGTPSLVLGGGDLFGSSPPVSNLLDDAP
                     TIEALGLMGLRYSALGNHELNDGVRDFLRTQYGGCVPGGACAGPAPFQYLAANVVDRT
                     TRQPVLPAYAIERVGRVEVGIIGVAFRDTPTIVTPAGVQSLEFLDEAESVNRAVRELR
                     SHGIETIVVLLHGSGSGAPEGGPITGPLVPIVEAMDGAVDVVITGHSEAGFVGTVDGK
                     LVTQAYSDGTAYADIDLTIDRKSGDVISKQAHLVRTWGDVAPGTTPHPQIAQLIQRSR
                     NSVAPLIDRVVGVAAAPITRDQTPAGESALGDLVADTQRAKGGTQLAFLNPGSLRADL
                     DVGEVTWGDLFAIQPFRNDLIRMRLTGAQLERLLEQQWEGQPQPRILQVSGLRYRWSP
                     DAPVGDRVRPEDVEVGGAPLDLAATYTVTVNNFIATGGDSFTVLLEGASREVVGGTDD
                     EAFVGYVEALPRPFTVSTDGRIALR"
     gene            complement(1804..2568)
                     /locus_tag="PLX85_04320"
     CDS             complement(1804..2568)
                     /locus_tag="PLX85_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62430.1"
                     /translation="MERLVRVGLFVGISAALLIFAACGGGDSKDSSSGEAAGTTAATA
                     SSSSSGGASSQAGTAVATGRATQAANTSGGGAGCKGTFSGGVTGSFESPGGTSAVNTD
                     YWFTEAELLQAARFLGSGEAQVKADIAAKKFVVYPLILNCGDSKTGVNFLAKADTYND
                     FPFGPKTYTIPSGGLLGGATNGEVGVIILVNDDPYKSKGGTFEVKKFDKTGIAGSFSI
                     DIEEAFAASGAPKTGKVTGTFDMACTGSTSRGGCSR"
     gene            complement(2658..3179)
                     /locus_tag="PLX85_04325"
     CDS             complement(2658..3179)
                     /locus_tag="PLX85_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62431.1"
                     /translation="MTISGGVSATIKTGGGPSSINSEYWLSDSERLQLAVAVGDTQAA
                     VQEKIAAGQFVLTPLLLTCGDTRTNVQLLATARRQDQLPFGAGSYRIAAGERLAAVTE
                     GEFAASVVLSGKAYRSGGGTLDITRFDRSGITGSFRLEIDDGGAAGAARGTVVGRFEM
                     SCTGVSTRGGCAR"
BASE COUNT          497 a         1296 c         1003 g          556 t
ORIGIN      
        1 cttggtcgac gagtacccac ccttctcgct gaagtaacca tcgagttgca tcaccgctgg
       61 gggccgctcg cagcagcggc ccccagcctg tatagacgta ggcatcgatc ccgtccgacc
      121 tgagggagcc ctcgatgcct cgtctcgctg cgcccgtcgc cgtcctggcg ctggccctcc
      181 tcgcgctctc tgtgctcccc gccgaggccg cgaagaaccg cccgctccac ctgcagctgc
      241 tcggcatcaa cgacctccga ggcgagctcg acccctcgac gctcgacgga cgccccgtcg
      301 ggggcgcagc cgtcctgggc acctaccttg cccaggcggt ccacgacgcg gatgccgagg
      361 gcacgccctc gctcgtcctc ggcggcggcg acctttttgg ctccagcccg cccgtctcga
      421 acctgctcga tgacgccccc accatcgagg cgctcggcct catggggctc cgctacagcg
      481 ctctcggcaa ccacgagctg aacgacggcg tccgcgactt ccttcggacg caatacggcg
      541 gctgcgtgcc gggcggcgcc tgcgctggcc cagcaccctt ccagtacctc gccgctaacg
      601 tcgtggaccg cacgacgagg cagccggttc tccccgccta cgccatcgag cgggtcggtc
      661 gcgtcgaggt cggcattatc ggtgtcgcct tccgcgacac cccgaccatc gtcaccccgg
      721 ccggggtcca atccctcgag ttccttgacg aagccgagag cgtgaatcgc gccgtgcgtg
      781 agctgcgctc gcacggcatc gagacgatcg tcgtcctcct ccacggctcg ggaagcggcg
      841 cccccgaggg cggcccgatc accggccccc tggtcccgat cgtcgaggcg atggacggcg
      901 ccgtcgatgt cgtcatcacc ggccattccg aggctggatt cgtcggcacc gtcgacggca
      961 agctcgtgac acaggcctac tccgatggca cggcctatgc cgacatcgac ctcaccatcg
     1021 accgcaagtc gggcgacgtc atctcgaagc aggcgcacct tgtccgcacc tggggcgatg
     1081 tcgcccccgg cacgactccg cacccgcaga tcgcacagct catccagcgc tcgaggaact
     1141 cggtcgcacc gctcatcgac cgcgtcgtcg gcgtggccgc cgcgcccatc acgcgcgacc
     1201 agacccccgc gggcgagtcg gcgctcggcg acctcgtcgc ggacacccag cgcgcgaagg
     1261 gtggcaccca gctcgccttc ctcaacccgg gcagcctccg cgccgatctg gatgtgggcg
     1321 aggtcacctg gggcgacctc ttcgcgatcc agccgttccg caacgacctg atccgcatgc
     1381 gcctgacggg tgcgcagctt gagcgcctgc tcgaacagca gtgggaaggc cagccccagc
     1441 cacgcatcct ccaggtgtcc ggcctgcgct accgctggtc acctgacgcg cccgtcggcg
     1501 accgcgtccg cccggaggac gtcgaggtgg gcggtgcgcc gctcgacctt gccgccacct
     1561 acaccgtcac ggtgaacaac ttcatcgcca ccggtggcga cagcttcacg gtcctcctcg
     1621 aaggcgcctc ccgcgaggtg gtcggtggta ccgacgacga agccttcgtg gggtacgtag
     1681 aggccctacc ccgcccgttc accgtatcca ccgacggccg catcgccctc cgctaatccc
     1741 ccgggacgaa gacgaagggc gcgagctcag ctcgcgccct tccctctctc caagccaccg
     1801 aggtcagcgc gagcagccgc cccgtgacgt cgatccggtg cacgccatat cgaacgttcc
     1861 cgtcaccttc cccgtcttcg gggctccgga ggcggcgaac gcctcctcga tgtcgatgct
     1921 gaagctgcct gcgatgcccg tcttgtcgaa cttcttcacc tcgaaggtgc cgcccttcga
     1981 cttgtagggg tcgtcgttca cgaggatgat gacgccgact tcgccgttcg tggctccgcc
     2041 gagcagaccc ccgctcggga tcgtgtacgt cttcggcccg aacggaaagt cgttgtacgt
     2101 gtccgccttg gccaggaagt tcacccccgt cttcgagtcg ccgcagttga ggatcagcgg
     2161 atagacgacg aacttcttcg ccgcgatgtc cgccttcacc tgtgcttcgc cgctcccgag
     2221 gaaccgcgcc gcctggagca actcggcttc cgtaaaccag tagtcggtgt tgaccgcgct
     2281 cgttccaccc gggctctcga acgacccggt gacgcccccg ctgaacgtcc ccttgcagcc
     2341 cgcgcctcca cccgaggtgt tcgcggcctg ggtcgcccgc ccggtcgcca cggccgtacc
     2401 ggcctggctc gaggccccgc ctgagctgct gctgctcgcg gtggctgctg tcgttccggc
     2461 cgcttccccc gacgaggagt ccttgctatc cccgccgccg cacgccgcga agatcaacag
     2521 cgcggccgaa atccccacga ataaccccac tcgcaccaac cgctccaacc gtctcctcct
     2581 tgcagacacg tgttcgttgc gctcagttgc cgcttcggaa ccgaagggat cgcgcgggtg
     2641 aaccgatcat cctcgcgtca tcgcgcgcac ccgccccggg tggacacacc cgtgcagctc
     2701 atctcgaaac gcccgacgac ggttccgcgg gcggcccccg ctgctccacc atcgtcgatc
     2761 tcgagtcgga atgagcccgt gatcccggag cggtcgaacc gcgtgatgtc gagggttcca
     2821 ccgccggatc gataggcctt gccgctcaac acgacgcttg ccgcaaactc gccttcggtc
     2881 actgcggcga ggcgctctcc agcggcgatg cggtacgaac cggcgccaaa cggcagttgg
     2941 tcctgtcggc gcgcggtagc cagcagttgg acgttcgtcc tcgtatcacc acaggtgagc
     3001 aagagcgggg tcagcacgaa ttgacccgcc gcgattttct cctgcacggc cgcctgcgtg
     3061 tccccaaccg cgaccgccag ctggagccgc tcggaatcgc tcagccagta ctcgctgttg
     3121 atcgacgaag ggccgccacc ggtcttgatc gtcgcgctca ccccgccgct gatcgtcact
     3181 tcgcacgtcc cgagggtgaa atcctctttg ctcgcggtcg tccgagtcgc cgtcgccgag
     3241 gctccttgcg cggtcgcccc tgcggcagaa ggtccagcgg tacctcctcg cgactgtctg
     3301 tctgcctggc cacccccgct tgagccacag ccgccgagga tcagcaccgc ca
//
LOCUS       DAORPG010000146         3347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2608_length_3347_cov_4.272479, whole genome shotgun sequence.
ACCESSION   DAORPG010000146 DAORPG010000000
VERSION     DAORPG010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2608_length_3347_cov_4.272479"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PLX85_04330"
     CDS             <1..328
                     /locus_tag="PLX85_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRC62432.1"
                     /translation="LYRIARNQLLDYLKRRRRVTVDVDLVNPAADIDLEAHAVAEDER
                     RLLRNALGHLSDDHRDVLVLRFLMEKSAAEVGLIMGRKEGTIRGLQFRALQALRSVME
                     ELGASR"
     gene            325..1665
                     /locus_tag="PLX85_04335"
     CDS             325..1665
                     /locus_tag="PLX85_04335"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRC62433.1"
                     /translation="MSATDFESVLDHCLDEVLNGRRTFESCLADWPQYADRLEASLRT
                     AMAVRASATSEASPVSARRAEFMAAVHTTPQQSRRRWGLRTPRLSFPTPVLLAPAALV
                     ATVALLIVLFRPFGSTSTAEAATLTTFGTVERTDGNAWQVVPDDAALHDGDRLRTSAD
                     SSAVLTFSDGSTVTLDPGTEIVLERGYRGTGKPIQLRQLAGRVWTNVATTDGSAFRLQ
                     TADVTVVPTAAASFETTVGDGETAVSAPEGNVEVQAPGERMLSAPGDVIRARLGQRLV
                     RLNALAVDRGFTVHLSGPLAASLVSPRGLATGATPDGFVFRQIPATLTTDPGGVEGDG
                     QRIMVLAPVEGTYTLLLRRTGDGAGTVLVRAGASEQRIEVPAIAPAYMARLRVTVSAS
                     TVSVQVEGAQSVLPARIERAERLVLSETARHHAAEVLKRLRDRRGQPGATPAGR"
     gene            complement(1670..2710)
                     /locus_tag="PLX85_04340"
     CDS             complement(1670..2710)
                     /locus_tag="PLX85_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRC62434.1"
                     /translation="MVGSEGVVPDLTVTRSQAIEAMVALAILLAGGAVAWLVQASLTS
                     LVSRINRSSSRSGVETLSRALRLPVATLIVATGAFIALRTMSWLDPHREELERAWTIA
                     VLVVAVVLLQRVAATSLGRLGEGRAGRAGALRQLTPLAQRAANLAILLIGTLVVLDQL
                     GVSISPLLAGLGIGGLAVALALQPLLTNLFAGWYVLSDASIREGDFITLLNGPAGNVT
                     DIGWRATRLRSADGESVIVPNGMLAAAVVTNAGAELARPVVVSFPVSLRSDLGRVEQT
                     MLAVLDSVVNDAEGAAPESAPQVRFQRVMDGRVECVLTIRARTASDVPALTHLVIKHV
                     QARLRAEGLEIA"
     gene            2828..>3347
                     /locus_tag="PLX85_04345"
     CDS             2828..>3347
                     /locus_tag="PLX85_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HRC62435.1"
                     /translation="MTIDDEPDAKSPRGVPALEDGGGSDRGLTAVIFDWGGTLAQFAT
                     IDLEDLWRLAARHLAPGRESEVATQLAAIEESFWERSSRDHRAWTLAELLAAARSSIG
                     VDVAEALLEEAGVLYLDAWTPHIRHEPDAVEVLQALRARGLRLGLLSNTHWPRAFHEH
                     FLERDGLNELLDA"
BASE COUNT          531 a         1220 c         1086 g          510 t
ORIGIN      
        1 gctctatcgc atcgcccgca atcagctgct cgactacctc aagcgccggc ggcgcgttac
       61 cgtcgatgtt gacctcgtga atccggccgc cgacatcgac ctcgaggcgc acgccgtcgc
      121 cgaggacgag cgtcgactcc tgcgtaacgc cctgggccac ctctccgacg atcaccgcga
      181 cgtgctcgtc ttgcgcttcc tgatggagaa atccgccgcc gaggtcgggc tcatcatggg
      241 acgcaaggaa ggcaccatcc gagggctcca gttccgcgcg ttgcaggccc tgcgttccgt
      301 catggaagaa ctgggggcgt cgcgatgagt gcaactgatt tcgagtcagt cctcgaccac
      361 tgcctcgacg aggtgctcaa tggccgacgc accttcgagt cgtgcctggc ggactggcct
      421 caatacgccg atcgtctcga ggcaagcctg cgcacggcga tggcggtcag ggcgtcagcg
      481 acttccgagg cgtcccctgt ctccgcgcgc cgtgccgagt tcatggccgc tgtgcacacc
      541 actccacagc aatcgcgacg ccgatggggc cttcgtacgc cgcgcctctc gttccccacg
      601 cctgtcctgc tggcacccgc ggcactcgtc gcaaccgtcg ccctgttgat cgtgctcttc
      661 cgccccttcg gttccacttc gaccgccgag gcggcgaccc tcacgacgtt cggcacggtc
      721 gagcgaaccg acggcaacgc gtggcaggtc gtgccggacg atgccgcgct ccacgatggc
      781 gaccgtctac gtacgtctgc tgatagcagt gcagtgctca cgttctcgga tggcagcacg
      841 gtcaccctcg acccggggac ggagattgtg ctcgaacgtg gctaccgagg cacgggcaag
      901 cccatccagc tccgccagct cgccggtcgt gtctggacga acgttgcgac cactgacggc
      961 agcgcgttcc gcctccagac cgccgatgtc accgtcgtgc ccacggcggc cgcgagcttc
     1021 gagacgacgg tcggcgacgg cgagaccgcg gtcagtgccc cggagggaaa cgtcgaggtg
     1081 caggccccgg gcgagcggat gctctccgcg ccgggcgatg tgatccgtgc ccgcctgggc
     1141 cagcggctgg tgcgcctgaa cgccctcgcc gtggaccgcg gcttcaccgt ccacctgagt
     1201 ggccccctgg cagcctcgct cgtcagcccc cgcggcctgg ccaccggcgc gacgccggac
     1261 ggcttcgtat tccgtcagat accggccacg ctcacgacgg accctggagg cgtcgagggg
     1321 gacggccagc gcatcatggt gctcgcgccc gtcgagggga cctacacact gctgcttcgc
     1381 cggaccggcg atggagccgg tacggtactg gtgcgtgccg gagcctctga gcagcgcatc
     1441 gaggtgccgg caatcgcgcc ggcatacatg gcccggctgc gcgtcaccgt aagcgcgtcg
     1501 accgtttcgg ttcaggtcga aggtgcgcag tccgtgctgc cggcacggat cgaacgggcc
     1561 gagcgcttgg tgctgagcga aaccgcgcgc catcacgccg ccgaagtgct gaagcgactt
     1621 cgtgatcgcc gaggacaacc cggcgccacg cccgcgggtc gctgacggct caggcgattt
     1681 caagcccctc cgctcgcagc cgggcttgga cgtgcttgat caccaggtgg gtgagcgccg
     1741 ggacatccga ggccgtgcgc gcacgaatgg tcagcacgca ctcgacgcgc ccgtccatca
     1801 cgcgctgaaa tcggacctgt ggcgcgctct cgggcgccgc gccctcagcg tcgtttacga
     1861 cactgtcgag gacggcgagc atcgtttgct cgacgcggcc gaggtcactg cgcagcgaca
     1921 ccggaaacga caccaccacg gggcgcgcca gttcggcgcc ggcattcgtg accacggccg
     1981 ccgccagcat cccgttcggc acgatgaccg actcgccgtc cgcgctgcgc agccgcgtgg
     2041 ctcgccaccc gatgtccgtt acgttgcccg cgggcccgtt caacagggtg atgaagtcac
     2101 cctcgcgaat cgacgcatcc gacagcacgt accaccccgc gaacaggttc gtcagcagcg
     2161 gctgcagcgc cagtgcgacc gcgaggccgc cgatccctag gcccgccagc agcgggctga
     2221 tcgagacgcc gagctggtcc agcacgacca gcgtgccaat cagcaggatc gcgaggttcg
     2281 ccgcccgctg tgccagcggt gtcagctggc gcagtgcgcc agctcgaccg gcgcgccctt
     2341 cccccagccg cccaagggac gtggccgcca cccgctgcag gagcacgacc gccaccacga
     2401 ggacggcgat cgtccacgcc cgctcgagct cctcccgatg cgggtccagc caggacatcg
     2461 tccgcaacgc aatgaaggcc ccagtcgcga cgatcagcgt cgccaccggc agccgaagtg
     2521 cgcgcgagag cgtctctact ccgctgcgcg aggacgaacg gttaattcgc gacacgagcg
     2581 aggtgagcga ggcctgcacg agccatgcga cggcgccacc cgccagcagg atggcaagcg
     2641 cgaccattgc ctcgattgcc tgtgagcggg tgaccgtgag gtccggcaca acgccctcgc
     2701 ttccgaccac cgatcctatg cgcccgacac accatgaaca cgcgtcgctc gtttcgccct
     2761 gcctatactc gccgctgtca gaagatccct tgtcgggccc ggtccccacg caaggagaag
     2821 aggcagcatg acgatcgacg atgagcccga cgcaaagtcg ccgagaggcg tccccgcgtt
     2881 ggaagacggc ggcggatcgg atcgggggct gacagccgtc atcttcgact ggggcgggac
     2941 gctcgctcag ttcgccacca tcgacctcga ggacctctgg cgcctcgccg cgcgtcacct
     3001 cgccccgggg cgcgagagcg aggtggccac gcagcttgcc gccatcgagg agtccttctg
     3061 ggagcgctcc agtcgcgacc atcgagcctg gacgctcgcc gagctgctcg cggccgcacg
     3121 ttcctccatt ggcgtcgacg tggcggaggc gctgctcgag gaggccggcg tcctctacct
     3181 cgatgcctgg acgccccaca tccgtcacga gccggatgcc gtcgaggtgc tgcaggcact
     3241 gcgggctcgc gggcttcgcc tcggtctgct ctccaatacc cactggccgc gagcgttcca
     3301 cgagcacttt ctcgagcgcg atggcctgaa cgaattgctc gatgccc
//
LOCUS       DAORPG010000147         3339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2627_length_3339_cov_3.629111, whole genome shotgun sequence.
ACCESSION   DAORPG010000147 DAORPG010000000
VERSION     DAORPG010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2627_length_3339_cov_3.629111"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..1379)
                     /locus_tag="PLX85_04350"
     CDS             complement(30..1379)
                     /locus_tag="PLX85_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HRC62436.1"
                     /translation="MPKAVELEAGGRVVAITNPDKVYFPERGYTKLDVARYFLAVGEA
                     ALRGVYHRPMVLKRFVNGATEEPFFQKRAPASRPEWISTARITFPSGRHADLAVCDEV
                     ADLLWAANLGCLDLNPWPVRADDVDHPDELRVDLDPTPEASWADVRDVALVVGEVLDD
                     LGYRGYPKTSGSRGIHINVRVEPKWEFSQLRRCALALAREVERRAPELATTAWWKEER
                     HGVFIDYNQNARDRTVASAYSIRPTPDARVSCPLEWSEVSNVDPAAFTLATVPQRLRE
                     HGDPHATIDDMAFSLEPLLEVVARHEREGLGDAAWPPQFPKMPGEPKRVQPSRARADD
                     EDGAPAKKSGRRQPVHPLITVANAQHKDEALAGLERWKARHPEAAAHLETADVLVDSM
                     RGMSSTWTRIRLNLRHVPVAQRPSEEPPDPDYDPWEGLDPSLRGRQTKRINAADGRI"
     gene            complement(1425..1889)
                     /locus_tag="PLX85_04355"
     CDS             complement(1425..1889)
                     /locus_tag="PLX85_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HRC62437.1"
                     /translation="MSDVFGRLSRSIAQLSGSPIAFLIAVLIIIVWAVAGPVFGFSDT
                     WQLVINTGTTIVTFLMVFLIQNTQNRDSTAVQLKLDELIHATGAARNEMIALEEDSEA
                     RLTREQERFRQVAAELNEASGDALEAARLVGGDTDAEERETGSTASVNDSGS"
     gene            complement(1903..2835)
                     /locus_tag="PLX85_04360"
     CDS             complement(1903..2835)
                     /locus_tag="PLX85_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62438.1"
                     /translation="MLFDGDKLSCLMCGYEHASRPRGMRSAGDLLDALPPLRADLIGH
                     ADSLGAQPERGSPGHLELRSASEVEANALQYGRLYAYQCLISSGHGLIALARGLEPPT
                     PEYGTWSIARTILDSSSLASWILEADISRRERARRALTVQLADLEAEHCFLTALLAHR
                     PDEEDADGFADLLAGTRARRQELLSASAELGAMAVPEPVVRAQLLGAEFEYVMCSSIL
                     EGRPWALLHATSIQSGYETDRVRSILATQVQLSASWYATAVWAYVSWMSRESLPELQS
                     SLEFGYDSLGLPDDAEARFWRRPREVRQQALAAS"
BASE COUNT          481 a         1165 c         1137 g          556 t
ORIGIN      
        1 cgcgcagcaa cccgatcact gagtcgaggc tagatgcggc cgtctgccgc gttgatgcgc
       61 ttcgtctggc ggccgcgtag cgacgggtcg aggccttccc acgggtcgta gtcggggtca
      121 ggcggctcct cggacggtcg ctgcgccacc ggcacgtgcc gcaggttcag ccggatgcgc
      181 gtccacgtcg aggacatgcc ccgcatcgag tcgaccagca cgtccgccgt ttcgaggtgt
      241 gctgccgcct cggggtgacg cgccttccac cgctcgaggc ccgcgagcgc ttcgtccttg
      301 tgctgcgcgt tcgccacggt gatcagcgga tgcaccggct gccgccgccc gctcttcttc
      361 gcgggggcac catcttcgtc gtccgcgcgc gcccgcgagg gctgcacgcg cttcggctcg
      421 cccggcatct tcgggaactg cggcggccac gcggcatcgc cgagcccctc acgctcgtgg
      481 cgcgcgacca cctcgagcaa tggttcgagg gagaacgcca tgtcatcaat cgtcgcgtgc
      541 gggtcgccgt gctctcgaag ccgctgaggc accgtcgcga gggtgaacgc ggccggatcg
      601 acgttgctca cctcggacca ctcgagtggg cacgataccc gcgcgtccgg cgtcggccgg
      661 atcgagtacg ccgaggcgac cgtgcggtcg cgtgcgttct ggttgtagtc gatgaagacg
      721 ccgtgccgct cctctttcca ccacgcggtg gtcgcgagct ctggcgcgcg gcgctccacc
      781 tcccgcgcga gcgcgagtgc acagcgccgc agctgactga actcccactt cggctcgacg
      841 cgcacgttga tgtggatccc gcgcgagccc gaggtctttg ggtagcctcg gtagccgagg
      901 tcgtcgagca cttcccccac gaccagcgcg acgtcgcgca cgtccgccca gctggcttcg
      961 ggggtggggt cgaggtcgac ccgcagctcg tcggggtggt cgacgtcgtc ggcgcgcacc
     1021 ggccaggggt tgaggtccag gcatccgagg ttcgcggccc acagcaggtc cgcaacctcg
     1081 tcgcacaccg ccaggtcggc atggcggccg ctgggaaagg taatgcgcgc cgtcgagatc
     1141 cattcgggcc ggctggccgg agcgcgcttc tggaagaatg gctcctcggt cgccccgttc
     1201 acgaagcgct tcagcaccat cggccggtgg tacacgcctc gcagtgccgc ttcgccgacc
     1261 gcgagaaagt agcgcgcgac atcgagcttc gtgtagcccc gctccgggaa gtacaccttg
     1321 tccgggttcg tgatcgcgac gacccgtccg ccggcctcga gttcgaccgc cttcggcatg
     1381 ggtctactct acgccccgtc gctgcccatg ggcgccggga tcgctcagga gccgctgtcg
     1441 ttgacgctcg ccgtgctgcc cgtctcgcgc tcttcggcgt ccgtgtcccc cccgaccagg
     1501 cgggcggctt cgagggcatc ccccgatgcc tcgttcagtt cagcagcgac ctgccggaag
     1561 cgttcctgtt ctcgggtcag ccgcgcctcg gagtcctctt cgagggcgat catctcgttg
     1621 cgtgcggccc ccgtcgcgtg gatcagctcg tccagcttca gctgcacggc cgtcgaatcg
     1681 cggttctggg tgttctggat caggaagacc atgaggaacg tcacgatcgt cgtgcccgtg
     1741 ttgatcacca gctgccacgt gtcgctgaac ccgaagaccg gccccgctac cgcccagacg
     1801 atgatgatga ggacggcgat caggaaggcg atgggcgagc cggatagctg cgcgatggat
     1861 cgcgacagcc gtccgaacac gtcgctcata gcggcgccgc cgctagctcg ccgccagggc
     1921 ttgctggcgg acctcgcggg gtcgtctcca gaaccgggct tcggcgtcgt ccggcagtcc
     1981 gagcgagtcg tatccgaact cgagggacga ctgtagctcg ggcagcgatt cccggctcat
     2041 ccagctcacg tacgcccaca ccgcggtcgc gtaccagctc gccgagagct gcacctgcgt
     2101 ggcgaggata gagcggacac ggtcggtttc gtatccggac tggatcgagg tcgcatggag
     2161 cagcgcccag ggccgccctt cgaggatcga gctgcacatc acgtattcga actcggcgcc
     2221 gagcaactgc gctcgcacaa cgggctcagg caccgccatc gcgccaagtt ccgcgctggc
     2281 ggagagcagt tcctggcgac gcgcgcgggt gccggccagc aggtcggcga agccgtccgc
     2341 gtcctcctcg tccggtcggt gcgccaggag cgccgtcaag aagcagtgct ctgcctcgag
     2401 gtccgcgagc tgcacggtca gtgcccggcg cgcgcgctcc cgacgtgaga tgtcggcctc
     2461 aaggatccag ctggcaaggc tcgatgagtc gaggatggtc cgagcgatgc tccacgtccc
     2521 gtactcaggg gtgggcggct ccagcccgcg cgcgagcgcg atgagcccgt gaccgctcga
     2581 aatcaggcac tggtaggcgt acagccggcc gtactggagc gcgttcgctt cgacctcgct
     2641 ggccgaccgc agctcgaggt ggccggggga cccccgctcc ggctgcgcgc cgagcgagtc
     2701 tgcatgtccg atcaggtccg ctcgcagcgg gggcagcgcg tcgagcaggt cgccggcgct
     2761 ccgcatgcca cgaggtcgcg aggcgtgctc gtacccgcac atgaggcagg agagcttgtc
     2821 tccgtcgaac agcaaccgcg cggcgcatcg cgggcagtcg gaatccgccg ctccgtcggc
     2881 ccgtcgcgcc cggttcgccg gccgcctggc cgtgtcccgc cgcgcttgat cgatgttgcg
     2941 aatctccgca ccccgcgcca tagctcctcc ggctctcgtt gacgctcaat ccctgccaga
     3001 gactgacggt aggtttgctc gctgccagaa cgcataacga cggcgaacga agcggttgcg
     3061 atgagataac acgccgaaaa cgggccgttg ggtcgtctgc gcacgagtgc acggcgcagg
     3121 ccactccttg gcgttcgcac atctgtccca atcctgggga cgccggcccg ccggggatta
     3181 ggcgactcgt tatgtgctcg caagccgcgt cgtcggctcg tggctggacg gcaagcgggc
     3241 ctccatatgg tgggaatgac cgcgagatgg gaggtcctga tggacggcca aacgcgcggc
     3301 gaactgttgg cgcagagttc cccaatgccc atttgctcc
//
LOCUS       DAORPG010000148         3312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2664_length_3312_cov_3.338962, whole genome shotgun sequence.
ACCESSION   DAORPG010000148 DAORPG010000000
VERSION     DAORPG010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2664_length_3312_cov_3.338962"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PLX85_04365"
     CDS             complement(<1..762)
                     /locus_tag="PLX85_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62439.1"
                     /translation="MPADVARRGFVAFVLTAIGILAALLAGSRGSSALGLEATWSISG
                     RLDASAFRAGSQLPAAGQGFEVISFGAAGPTAALSTYRYGGPDLTPTPQRRFVTCAAS
                     AAAGRLTLNIGNAFPYAGCVFFPTVTNTGTSAIEVDLGSLQQPAGVFCGRSTCKVDIV
                     AGGTDEAGVADRCISTGSVARASTSLLTYRLASGASMTCPVFVVLLQPASEATTYAVT
                     VDAPRPQAPAPTTYIDVGPAPPLVPGAAATPRPPRT"
     gene            complement(762..1457)
                     /locus_tag="PLX85_04370"
     CDS             complement(762..1457)
                     /locus_tag="PLX85_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62440.1"
                     /translation="MAESAPLMRPRRRLLRRLYPVAAIAGLLLFAVVALAYRQAIRPV
                     SALWSQDLTIDASISVAAQNQTLYLRCSTGATACTVNNTTPPASNVLVGTNPSPNGGS
                     ASLGNNASVTLKSASLTRINSRKSWTVNLRITGTAVTTGQVRLWYVTPASSACGTPPA
                     ASATNYIAGASLASPAFPVGTVVVPLTAGTATTGVPVSTVALCLRVDRTGGGGNVVIS
                     TTGLSTVVGPFTP"
     gene            complement(1436..2608)
                     /locus_tag="PLX85_04375"
     CDS             complement(1436..2608)
                     /locus_tag="PLX85_04375"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRC62441.1"
                     /translation="MALLAVAALAGGNAFAFEVIPGQVTALWGPYGLAGAGWLLAAMI
                     ALVAARLVPPLELGQQADRTQVLQLAAIVGVYLVVVQLLVGAFAGFGRSPFDHSPEWL
                     AVNSFFGFAPLLAGEAARLALLRSLGSASLTLALLVASLLVVIARLPLESFGVNGFPA
                     QAEFWGGTFIPLTAVGLVAGIYALYGGLRASLLVTAPLVAFHYLSPALPGGDWPLLAL
                     AGVAGPATALWMSESVFTADEASAVTATRGGPFSRLGAPSVGWTVTAVIALAIFWFTF
                     GFYGFQPAFIPSSSMSPLLNRGDVVLVGPVSADNVEVGDIVLYELGPHVKILHRVIEI
                     RRTPSGSRQFITKGDANNTDDLLPVEEQQLRGELLGSVPKVGWLPLKLSEWLRAHR"
     gene            complement(2680..>3312)
                     /locus_tag="PLX85_04380"
     CDS             complement(2680..>3312)
                     /locus_tag="PLX85_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62442.1"
                     /translation="PAAPPHSSEQSMYVNRRMLGALMIVALLATALLGAAGTTWYEGL
                     TASASVATTSLDASVSCSSLSETDPNNNVAPTSAANGTYGWQLTLPGAYAGYALSCNY
                     TITNTAPVAWHVETLTVVVTAPGGGTSSATCTLGASCAYTGTYFDLSIPDTRGCQVHQ
                     SPATPSTLTGAFTITFTDAPAASQSWSVKVTYRVQQWNESVYASCGVLRP"
BASE COUNT          560 a         1028 c         1230 g          494 t
ORIGIN      
        1 cgtacgtggc ggtcgtgggg tggcggctgc cccgggaacc agcggcggcg ccggacccac
       61 atctatgtac gttgtcggtg cgggcgcctg cggccggggc gcatcgaccg tgacggcgta
      121 ggtcgtcgcc tccgaggcgg gctgcagcaa caccacgaag acggggcagg tcatcgatgc
      181 tcctgatgcg agacgatatg tcagcaggga cgtgcttgcg cgggcgaccg atccggtaga
      241 tatacagcgg tcggcgacgc cggcctcgtc ggtcccgccg gcgacgatgt ccactttgca
      301 cgtcgaacgg ccgcagaaca cgccagcggg ttgctggagc gacccgaggt cgacctcgat
      361 ggcgctggtg cccgtgtttg tgacggttgg gaagaagaca cagcccgcgt atgggaacgc
      421 gttgcctatg ttgagggtga gtcgtcccgc ggcggcgctg gccgcacagg tgacgaagcg
      481 gcgctggggt gtgggagtga gatccgggcc gccgtagcgg tacgtgctca gcgcggcggt
      541 cgggccggcg gcgccgaagg agatgacctc gaaaccctgg ccggcggcag ggagctgact
      601 gcccgctcgg aacgcggacg cgtcaaggcg ccccgagatg ctccacgtcg cctccaggcc
      661 cagcgccgag gagcctcgag agcctgccag gagcgcggcc aggatgccga tcgcggtgag
      721 tacgaacgca acgaaccccc gccgagcaac gtcggctggc atcagggcgt gaacggtccg
      781 accacggtgg acagccctgt cgtggagatc acgacgttgc ctccgccgcc cgtgcggtca
      841 acacgcaggc atagggcgac cgtgctcacg gggacccccg tcgtcgccgt accagcagtc
      901 agcgggacca cgaccgtgcc gaccggaaat gcgggtgagg caagcgatgc gcccgcgatg
      961 tagttcgtcg cactcgccgc gggcggggtg ccgcacgcgg atgacgctgg ggtgacgtac
     1021 cagagtcgaa cctgcccggt cgtgaccgcc gtgccagtga tgcggaggtt gacggtccag
     1081 ctcttgcgac tgttgatccg ggtcaggctg gcagacttga gggtcacgga cgcgttattc
     1141 cctagcgagg cgctcccgcc gttgggtgag ggattcgtgc cgacgagcac gttactggcg
     1201 ggcggggtgg tgttgttcac cgtgcatgcg gtcgcgccgg tgctgcaacg caagtacaac
     1261 gtctggttct gagcggccac ggagattgac gcgtcgatgg tcaggtcctg cgaccagagc
     1321 gcgctcacgg gccgtatcgc ctgccggtac gccagtgcga cgacggcgaa caagagcagt
     1381 ccggcgatgg cggcgactgg atacaaccgg cgcaggagac gccgccgggg tcgcatcagc
     1441 ggtgcgctct cagccattcg gagagcttca gcgggagcca tccgaccttt gggacgctgc
     1501 cgagcagttc gccgcggagc tgctgttctt cgacggggag caggtcgtct gtgttgttgg
     1561 cgtctccctt tgtgatgaac tggcgactcc cggagggcgt ccgacggatt tcgatcacgc
     1621 ggtgaaggat cttgacgtgc ggtcccagct cgtagagcac gatgtcgccg acctcgacgt
     1681 tgtcggcgct gaccggcccg acgagcacga cgtcgcctcg gttcagcaag ggcgacatgg
     1741 agctgctggg gatgaatgct ggctggaagc catagaaccc gaacgtgaac cagaagatcg
     1801 cgagcgcgat cacggccgtg acggtccagc ccacggacgg agccccgagt ctggagaacg
     1861 gtccgcctcg agtggcggtg accgctgacg cctcgtccgc tgtgaacaca ctctcgctca
     1921 tccagagtgc ggtggccggt ccggcgacac ccgcgagcgc gagcagaggc caatccccac
     1981 cggggagtgc gggggagagg tagtggaagg cgaccagggg ggcagtgacc aggaggctgg
     2041 cgcgcaggcc gccatagagc gcatagatcc ccgctaccag accgacggct gtgagcggaa
     2101 tgaacgtacc gccccagaac tccgcctgag cggggaaccc gtttaccccg aagctctcga
     2161 ggggcagccg cgcgatcacc accaggaggc tcgcgacgag cagcgcgaga gtgaggctcg
     2221 cgctgccgag tgaacggagg agcgcgaggc gcgcggcctc gccggccaga agcggcgcga
     2281 acccgaagaa cgagttgacg gcgagccatt ctggggagtg gtcgaatggg gagcggccga
     2341 acccggcgaa agcccccacc agcagctgca cgacgaccag gtacacgccg acgatcgcgg
     2401 cgagttgaag cacctgcgtg cgatccgcct gttgcccgag ttccagcggc gggaccagtc
     2461 gggcggcaac cagcgcgatc atcgcggcca ggagccatcc ggcgcctgcc aggccgtacg
     2521 ggccccacaa cgctgtgacc tgtcccggga tcacctcgaa ggcgaacgca ttcccgccag
     2581 ccagcgctgc cacggccagc agagccacga gcacgcctcg ggccgggaat ggccggggtt
     2641 ggtgggaagc cgtggccctc aggtcgacgc tcgcctggct catgggcgca gcactccgca
     2701 ggaggcgtag acgctttcgt tccactgctg gacgcggtac gtgactttca cgctccagct
     2761 ctgggaggcg gctggcgcgt cggtgaaggt gatcgtgaat gctcccgtga gcgtcgatgg
     2821 ggtggcgggc gactggtgca cctggcatcc gcgcgtgtca gggatgctca ggtcgaagta
     2881 ggtgccggtg tacgcgcagg atgctccgag ggtgcaggtg gcgctcgagg tgccgccgcc
     2941 gggagcggtg accacgaccg tgagcgtctc gacatgccag gcaacgggtg cggtgttggt
     3001 tatggtgtag ttgcagctca gcgcgtagcc ggcgtacgcg ccgggcaggg tgagctgcca
     3061 gccgtatgtg ccgttggccg ctgatgtcgg cgcaacgttg ttgttggggt ctgtctcgct
     3121 cagtgaggag caggagacgc tcgcgtcgag cgaggttgtt gcgacgctgg cagacgccgt
     3181 aaggccctcg taccaggtgg tgcccgccgc acccagcagc gccgtggcga ggagcgccac
     3241 aatcatgagc gctccaagca tccggcggtt cacatacatc gactgctccg aggagtgagg
     3301 cggggccgcc gg
//
LOCUS       DAORPG010000149         3298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2687_length_3298_cov_3.546716, whole genome shotgun sequence.
ACCESSION   DAORPG010000149 DAORPG010000000
VERSION     DAORPG010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2687_length_3298_cov_3.546716"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..80)
                     /locus_tag="PLX85_04385"
     tRNA            complement(5..80)
                     /locus_tag="PLX85_04385"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44..46),aa:Glu,seq:ttc)
     gene            239..1708
                     /locus_tag="PLX85_04390"
     CDS             239..1708
                     /locus_tag="PLX85_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HRC62443.1"
                     /translation="MTATDDRTDLRTVDEYQSPVSGYQLLLRLRIQNTPGNLGKVTTA
                     IGEMGADIGSIDIVEARTEMIIRDFRLYCYDEDHARRVVARVAAVPGVEVQHASDRTF
                     QLHRRGKIEVNNKVHIRTMAELAHVYTPGVARVSMQIHDNPDAVWSLTIKPNTVAVVT
                     DGTAVLGLGDIGPMAAMPVMEGKSMLFKAFADIDAWPICLNTKDPDEIVRIVKAISPG
                     FGGILLEDISAPRCFEIEDQLKAELDIPVFHDDQHGTAVVVLAGVINSLKIIQKRPED
                     LKVVLLGVGAAGVACANILMNFGVKNVIGFDRAGAIYEGRVEGMNPAKERFARQTNRE
                     GFDGTVGEALQGADMFLGLSGPRMVTADQIAGMNPDSMVFALSNPEPEIMPELIQDKA
                     RIVATGRSDYPNQVNNVLCFPGLFRGAFDSAATTISEEMKIVAAKAIAETIPDNLLNE
                     DYILPSVFNPDVADRVAAAVADEALRSGRVRAKGSRVFV"
     gene            complement(1786..2388)
                     /locus_tag="PLX85_04395"
     CDS             complement(1786..2388)
                     /locus_tag="PLX85_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015184861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HRC62444.1"
                     /translation="MTLDVSRREMPHSTDKSPRLAPGQRLTDGFPVLTYGGVPKIDLA
                     TWQFRIFGLVEDEVTLNWEQFMALPQSKSHSDIHCVTTWSRYDNDWEGVRIADMLSLV
                     QVKPEAKHVMFHSYGGYTTNVPLDELLYNDALLAHTHDGAPLPLEHGGPLRGVVPHLY
                     FWKSAKWVRGIELMAEERPGFWEMYGYHIHGDPWKEERYS"
     gene            2510..3025
                     /gene="lspA"
                     /locus_tag="PLX85_04400"
     CDS             2510..3025
                     /gene="lspA"
                     /locus_tag="PLX85_04400"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRC62445.1"
                     /translation="MEETVIRAPSLRERLAASRSERGHLVPYLFIVLAVIVADQLTKA
                     LVRGRLSEGEAWPSKDALLAISHVENSGAAFGIFQGGGAFLLGAAAVGVAAIIVYMLL
                     APPAQRLYVAALSLILGGAIGNLTDRLAKGTVTDFIDPTHYPAFNIADSAIVCGVIAV
                     VLLSLREEARR"
     gene            3022..>3298
                     /locus_tag="PLX85_04405"
     CDS             3022..>3298
                     /locus_tag="PLX85_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRC62446.1"
                     /translation="MTELTRRFRAGRADRLDRLMADAVPDISRSQAQKLIEGGRVRVD
                     DAAASRPAHTVEVGSLVEVCLPERGVDPEVAASIPLDLLYEDEHVVII"
BASE COUNT          548 a         1202 c          976 g          572 t
ORIGIN      
        1 gcagtggtac ccccaagggg attcgaaccc ctgtttccac cttgaaaggg tggcgtccta
       61 gtcccctgga cgatgggggc gggtcggtcc tccgaggata gcgcgcccgc gctcgacggt
      121 catggccctc ggcgctgtgg cgcccgtcag cggccctgac agaccctccg gcccctgccc
      181 agaagatgcc tcgggggtat agtctcgatc actacttgct ggttgacgga gcaccaagat
      241 gaccgccacg gacgaccgaa ccgacctgcg tacagtcgac gagtatcaga gccctgtctc
      301 gggctatcag ctgctgctgc ggctgaggat ccagaacacg cctggcaacc tcggcaaggt
      361 cacgaccgcc atcggcgaga tgggcgcgga catcggctcc atcgacatcg tcgaggcccg
      421 caccgagatg atcattcgcg acttccgcct ctactgctac gacgaggatc acgcgcgccg
      481 cgtcgttgcc cgcgtcgccg ccgtgcccgg tgtcgaggtg cagcacgcct cggaccgcac
      541 cttccagctc caccgccgcg gcaagatcga ggtcaataac aaggtccaca tccgcaccat
      601 ggcggagctc gcccacgtct acactcccgg cgtcgcccgc gtctcaatgc agatccacga
      661 caatcccgac gccgtctggt cgctcaccat caagccaaac accgtcgccg tcgtcactga
      721 tggcaccgct gtcctcggcc tcggcgacat tggcccgatg gccgccatgc cggtcatgga
      781 gggcaagtcg atgctcttca aggccttcgc cgacatcgat gcctggccga tctgcctgaa
      841 caccaaggat cccgatgaga tcgtccgcat cgtaaaggcc atctccccgg gctttggcgg
      901 catcctgctc gaggacatct ccgcccctcg ctgcttcgag atcgaagacc agctcaaggc
      961 ggaactcgac atccccgtct tccacgacga ccagcacggc accgccgtcg tcgtcctagc
     1021 cggcgtcatc aacagcctga agatcatcca gaagcgtccc gaggacctga aggtcgtgct
     1081 cctcggcgtc ggcgccgccg gcgtcgcctg cgcgaacatc ctgatgaact tcggcgtgaa
     1141 gaacgtgatc ggcttcgacc gggccggcgc gatctacgaa ggtcgcgtcg agggcatgaa
     1201 ccccgccaag gagcgattcg cgcgccaaac caaccgcgaa ggtttcgacg gcaccgtcgg
     1261 cgaggcactg cagggcgcgg acatgttcct cgggctctcc gggccgcgca tggtcaccgc
     1321 cgaccagatc gccggcatga accccgactc gatggtcttc gcgctctcca acccggagcc
     1381 cgagatcatg ccggagctca ttcaggacaa ggcgcgtatc gtcgcgaccg gccgctccga
     1441 ctacccgaac caggtcaaca acgtcctctg cttcccgggc ctcttccgag gtgccttcga
     1501 ctccgccgcg acgaccatct ccgaggagat gaagatcgtc gccgccaagg ccatcgcgga
     1561 gaccatcccg gacaacctgc tgaacgagga ctacatcctc ccgtccgtct ttaacccgga
     1621 cgtggccgac cgggtcgccg ccgccgtggc cgacgaggcc ctccgctctg gtcgcgtccg
     1681 cgcgaagggt tcgcgcgtct tcgtctagcg caccctcccg accacataac gagagcgccc
     1741 ccggttgggg gcgctctcgt catctcccgc tcgtctggtt cgaggctagc tgtaccgctc
     1801 ttccttccac gggtcgccgt ggatgtggta cccgtacatc tcccagaacc ccggccgctc
     1861 ctcggccatg agctcaatgc cgcgcaccca cttcgcgctc ttccagaagt acaggtgcgg
     1921 caccaccccg cgcagcggcc caccgtgctc gaggggcaac ggggcgccgt cgtgggtatg
     1981 tgcgaggagc gcgtcgttgt acagcaactc gtcgagcggc acgttcgtcg tgtacccacc
     2041 gtaggagtgg aacatgacgt gcttcgcctc cggcttcacc tggacgaggg acagcatgtc
     2101 cgcgatgcgc acgccctccc agtcgttgtc gtaccggctc caggtggtca cgcagtggat
     2161 gtccgaatgg ctcttgctct gcggcaacgc cataaactgc tcccagttca gcgtcacctc
     2221 gtcctcgaca agcccgaaga tccggaactg ccacgtcgcg aggtcgatct tcggcacgcc
     2281 gccgtaggta aggactggga atccatcggt cagccgctgg cccggcgcca gccgcggcga
     2341 tttgtccgtc gagtgcggca tctcccggcg cgaaacgtcg agggtcattc gcaaacccct
     2401 tccttgctca atgctgttgc gtcagggtac cggcttgcga ggagcccctc aatgacgttt
     2461 ctgacgcctc cgccggatcg cagcgtccgg cttcgttacg atggatccca tggaagaaac
     2521 ggtgatccga gccccttcgc tgcgtgagcg gctcgccgcc tcgcgctccg agcgcggcca
     2581 cctggtgccg tatctgttca tcgtgctcgc cgtcatcgtc gccgaccagc tcacgaaggc
     2641 gctcgtccgc ggacggctct ccgagggaga agcgtggccc tccaaggacg cgctcctcgc
     2701 gatctcgcat gtagagaact cgggggcagc gttcggcatc ttccagggcg gtggcgcctt
     2761 cctgctcggt gccgccgctg tcggggtcgc agccatcatc gtctacatgc tgctcgcgcc
     2821 accggcgcag cgcttgtacg tggccgcgct ctcgctgatc ctcggcggag ccatcggcaa
     2881 cctcaccgac cgcctcgcga agggcaccgt gaccgatttc atcgacccca cccactaccc
     2941 cgcgttcaat atcgcggact ccgccatcgt ctgtggcgtc attgccgtcg tactgctcag
     3001 tctccgcgag gaagcgcgcc gatgaccgag ctaacacgtc gcttccgtgc gggccgcgcc
     3061 gaccgactcg accgcctcat ggccgacgcc gtccccgaca tctcgcgctc ccaggctcaa
     3121 aagctgatcg agggcgggcg cgtccgcgtc gacgacgcag cggcttcgcg tcccgcccac
     3181 accgtcgaag tcggcagcct cgtcgaggtc tgccttcccg aacgcggcgt cgatcccgag
     3241 gttgcggcct cgatcccact cgatctcctc tacgaggacg aacacgtcgt catcatcg
//
LOCUS       DAORPG010000150         3295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2694_length_3295_cov_3.387963, whole genome shotgun sequence.
ACCESSION   DAORPG010000150 DAORPG010000000
VERSION     DAORPG010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2694_length_3295_cov_3.387963"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /gene="atpA"
                     /locus_tag="PLX85_04410"
     CDS             complement(<1..1047)
                     /gene="atpA"
                     /locus_tag="PLX85_04410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRC62447.1"
                     /translation="MPVRVEEIASILKQQIEQFDSGVEAANVGTVIEASDGIARIHGL
                     GQCLTSELIEFANGTRGLALNLEEETVGAIILGNYTAIREGDEVKTTGLVASVPVGEA
                     LIGRVVNALGDPIDERGPVNTTERRPVERIAPDVVSRVSVNQPVQTGIKAVDSMVPIG
                     RGQRELIIGDRSTGKTAIALDAIINQRGGDLVCVYVAIGQKEAKVAQIVATLEQQGAM
                     GHTIVVSAGASESSALQYLAPYSGCAMAEYFMERGRDVLIIYDDLTKHAWAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERSARVDAAHGGGSITALPIIETQAGDVSAYIPTNV
                     ISITDGQIFLESDLF"
     gene            complement(1108..1656)
                     /gene="atpH"
                     /locus_tag="PLX85_04415"
     CDS             complement(1108..1656)
                     /gene="atpH"
                     /locus_tag="PLX85_04415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HRC62448.1"
                     /translation="MAGLARDIAGRRYALALSAIAAEQNSFARWAEVVDALDALTSEP
                     RFVNALQSDGMTDEKLQAIVRQVLPDIAPVEMNLFRLLRRKNRLALGPSIASYFHEMW
                     DEERGVERAVVRTAVELDGETRDRMRQQLSQQTGKTIELETEVDPSLIGGAVIRIGDR
                     LIDGSTRTRLRQLRGRLQQGAL"
     gene            complement(1663..2277)
                     /gene="atpF"
                     /locus_tag="PLX85_04420"
     CDS             complement(1663..2277)
                     /gene="atpF"
                     /locus_tag="PLX85_04420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRC62449.1"
                     /translation="MALKSLVRRTGKRRFGALLAGASLALTPAFAAAAESSGGIAALG
                     ISLPGLIQNLVNFTILLVVLRLFLYKPLFRVLDERKRKIQEGIDRASAAANTAAESEA
                     EARRILDQARAEGQESVRRAQESAARLREELEARARQDADQIVARAREEVALERDQAI
                     EQLRREFADLTIVAAERVINQSLDRQAHQRLIDEVLVNSQAGRN"
     gene            complement(2358..2573)
                     /gene="atpE"
                     /locus_tag="PLX85_04425"
     CDS             complement(2358..2573)
                     /gene="atpE"
                     /locus_tag="PLX85_04425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRC62450.1"
                     /translation="MKYLAAAISMAVGAIGPGIGIGLLGGKAMEALGRNPEAAGAIQT
                     NMILAIAFAEAIGIYALVVAILIGFVF"
     gene            complement(2780..>3295)
                     /locus_tag="PLX85_04430"
     CDS             complement(2780..>3295)
                     /locus_tag="PLX85_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRC62451.1"
                     /translation="GGAPAVTAASEEAGAKEGEFSGLLSPWFRSVNTDVNAPLAIAIF
                     SFIFVEFWGLSALGFGYLKKFFNFGRLLHGNPMGVIDVFVGVLELVSELSRMVSFTFR
                     LFGNVFAGEVLLAMMTFLVPFVLVNVFYGLELFVGLIQAFVFAMLTLVFAQTAVTSHD
                     SHEGEHGEAHH"
BASE COUNT          534 a         1040 c         1188 g          533 t
ORIGIN      
        1 gaagaggtcg gactcgagga agatctggcc gtcggtgatc gagatgacgt tcgtcgggat
       61 gtacgccgac acgtcgccag cctgcgtctc gatgatcggg agggcggtga tggaaccgcc
      121 gccgtgggcc gcgtccacgc gagcggagcg ctcgaggagg cgggagtgga ggtagaaaac
      181 gtcgccggga tacgcctcgc ggccgggggg gcggcggagg aggagcgaaa tctggcggta
      241 cgcccaggcg tgcttcgtca ggtcgtcgta gatgatgagg acgtcgcgac cacgctccat
      301 gaagtactcg gccatggcgc agccggagta gggggcgagg tactgaaggg cggacgactc
      361 ggaggcgccg gcggagacga cgatggtgtg gcccatcgca ccctgctgct cgagggtcgc
      421 gacgatctgg gcgaccttcg cttccttctg gccgattgcg acgtagacgc agacgaggtc
      481 gccgccccgc tggttgatga tggcgtcgag ggcaatggcg gtcttgccgg tcgagcggtc
      541 gccgatgatg agctcgcgct gacctcggcc gatcgggacc atcgagtcga cggccttgat
      601 gcccgtctgg acgggctggt tcacggagac acgggagacg acgtcggggg cgatgcgttc
      661 gacggggcgg cgctccgtgg tgttgaccgg gccgcgctcg tcgatggggt cacccagcgc
      721 gttgacgacg cggccgatca gggcctcgcc gacggggacg gaggcgacca ggccggtcgt
      781 cttgacctcg tcaccttcgc ggatggccgt gtagttgccc aggatgatgg cgccgacggt
      841 ctcttcctcg aggttgagcg cgaggccgcg agtgccgttg gcgaactcga tgagctcgga
      901 ggtcaggcac tggccgagac cgtggatgcg ggcgatgccg tcactggcct cgatgaccgt
      961 gccgacgttc gcagcctcga cgccggagtc gaactgctcg atctgctgct tgaggatcga
     1021 cgcgatctct tcgacgcgta cgggcatctg gcacccctcc ctgcgtccgc cgctgcccgc
     1081 agcggacgct ctgtctgcct ggccttgcta cagcgcgccc tgttgcaggc gtccgcgcag
     1141 ctggcgcagt cgtgtgcgcg tcgacccgtc gatgaggcgg tcgccgatgc gaatgacagc
     1201 accgccgatg agcgaagggt cgacttcggt ttccaattcg atggtcttgc cggtctgctg
     1261 cgagagctgc tggcgcatcc ggtcgcgagt ctcaccgtcg agctcgacgg cggtccgcac
     1321 cacggcccgc tccaccccgc gttcctcgtc ccacatctcg tggaagtacg aggcgatgga
     1381 cgggccgagc gccaggcggt tcttgcgccg cagcaggcgg aacaggttca tctcgacggg
     1441 cgcgatgtcg ggcaggacct gccggacaat ggcctgcagc ttctcgtcgg tcatcccgtc
     1501 cgactgcagc gcgttgacga accgcggctc ggacgtgagc gcgtcgagcg cgtcgacgac
     1561 ctctgcccag cgggcgaagg aattctgctc cgcagcgatc gcgctgagcg cgagcgcgta
     1621 gcgccgtccg gcgatatcgc gggcgaggcc tgccatgtcg agctagttcc ttccggcctg
     1681 gctgttgacc agcacctcgt cgatgagtcg ctggtgagcc tggcggtcga gggactggtt
     1741 gatgacgcgc tcggcggcga cgatcgtgag gtcagcgaac tcacggcgca gctgctcgat
     1801 ggcctggtcg cgttcgaggg caacctcttc gcgggcgcgg gcgacgatct gatcggcgtc
     1861 ctgccgcgcg cgtgcctcga gctcctcgcg gaggcgagcc gcggactcct gcgcccgccg
     1921 cacggactcc tgaccctccg cacgtgcctg gtccaggatg cgccgagcct cggcctcact
     1981 ttcggcggcg gtgttcgccg ccgcggaggc gcggtcgatg ccttcctgga tcttgcgctt
     2041 gcgctcgtcc agcacgcgga agagcggctt gtagaggaag agcctcagga cgacgagcag
     2101 gatcgtgaag ttcacgaggt tctggatgag accgggcagg ctgataccga gggcggcgat
     2161 gccaccactc gattcagcgg ccgcggcgaa cgctggcgtc agcgccaggc tggcgccggc
     2221 gagcagcgcg ccgaagcgcc gcttcccggt ccgtcgtaca aggctcttga gggccattgc
     2281 gtacgctccc cgatgttcgc gtatcccaga ctcccgcggg cgtgcctcga gggtgcgccc
     2341 gcgggagcgg agtcggttta gaagacgaag ccgatcagga tggccacgac cagggcgtaa
     2401 ataccgatgg cctcggcgaa cgcgatggcg aggatcatgt tcgtctggat ggcgccggcg
     2461 gcctccgggt tacggccgag ggcctccatc gccttgccgc cgagcaggcc gatgccgatg
     2521 ccggggccga tggcgccgac ggccatcgag atggcagcgg cgaggtactt catgctgctc
     2581 gccgtgaagt cgttgtcagc ggcggccgcc tgctgggcgc ccgcaacccc tgttgccgcg
     2641 agccagagtc ctgcagccgc gagcgcgact acgaaacccc gccgggcgat cgtgccagcc
     2701 ttcatttgag atcctcctcc tggatatgcc cgcacgaatg cgccgcgcgg ccgcccctat
     2761 ctcctctttg aacaggcgat cagtgatgtg cctcaccgtg ctcgccctcg tggctgtcgt
     2821 gtgaggtgac agccgtctgg gcgaacacga gcgtgagcat cgcgaagacg aatgcctgga
     2881 tgaggcccac gaagagctcc aggccataga agacgttcac gagcacgaac ggcacgagga
     2941 acgtcatcat cgcaagcagg acctctccgg cgaagacgtt gccgaagaga cggaaggtga
     3001 acgaaaccat gcgcgagagc tccgagacga gctcgagcac tccgacgaag acatcgatga
     3061 cgcccatggg gttgccgtgg agcaggcgtc cgaagttgaa gaacttcttc aggtagccga
     3121 agccgagggc agagaggccc cagaactcga cgaagatgaa cgagaagatc gcgatggcga
     3181 gcggggcgtt cacgtcagtg ttgacggagc ggaaccacgg gctcaggaga cccgagaact
     3241 cgccttcctt cgcgccggcc tcctcggagg cggcggtgac agccggggcg ccgcc
//
LOCUS       DAORPG010000151         3292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2701_length_3292_cov_3.700031, whole genome shotgun sequence.
ACCESSION   DAORPG010000151 DAORPG010000000
VERSION     DAORPG010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2701_length_3292_cov_3.700031"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLX85_04435"
     CDS             complement(<1..223)
                     /locus_tag="PLX85_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62452.1"
                     /translation="MSIGSTEVEPQVAQGAASAVARPRFFYGWRLVGAAFVASFTTAG
                     VQAYVAGAFFVPMSDDLGWTRAQFLYGQTV"
     gene            complement(307..966)
                     /locus_tag="PLX85_04440"
     CDS             complement(307..966)
                     /locus_tag="PLX85_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRC62453.1"
                     /translation="MRIGILSDTHTGRPLDEFGPGPGEFLASVDLIVHTGDVTGPLVL
                     DWMEQFGPLIVNRGNHDTFDDPRLEDVVLLEREGWRMGMAHIVLDHRHRGEERVGHLK
                     RRGYGDTELNILIAGDTHYERLEYEDNTLIINSGSLTLPHNQTVRLGSVVLLEVTRDH
                     VHAEIIPLGESPGLKNPTRPAHVDVERGGRLLSASYDGEPIELTNGTLHWPGQGPRDQ
                     M"
     gene            complement(1038..2315)
                     /locus_tag="PLX85_04445"
     CDS             complement(1038..2315)
                     /locus_tag="PLX85_04445"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRC62454.1"
                     /translation="MPGPLAGIKVLDVTQVVAGPFCGVVLADLGADVIKVEPPGGEST
                     RVNGAFIPGESKGYHSLNRGKRSLVVDLTKAEGKALIHRLMPSVDIFIINARPSVPAK
                     LQLDYATLSALRPDLIYIENTGFGTEGPGADRSGSDIVAQAYSGLMAGDAKIDEWGAP
                     QMITATAPADYLAAVSAAVGACAALFHRSLTGEGQKVSTSLLQSGLVLQGSFVAKLPV
                     FDAMAIGPMFDRVQEARAQGQSYEEQLAIRAQAGILNKAFRLWYGGYRVKDGALILGA
                     LTPLNQQQMRQAIGLEDDPTASPDFNALDPANDIVVDQVRERIRNIMLTRTMDEWIER
                     FDSLGAPVSKVNFPEELAYDPQVEAMGYMMDFDHPLTGPERMVGPVVQLSKTPVGSTR
                     PSPPLGWHTDEVLGEFGLTADEIAGLRAGGVVH"
     gene            2485..>3292
                     /locus_tag="PLX85_04450"
     CDS             2485..>3292
                     /locus_tag="PLX85_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRC62455.1"
                     /translation="MEIRQMGRSGLRVSVVALGGNTFGPAVDAAQTANILAAAHDRGI
                     NAVDTADIYNNGMSEEHIGKAVAGNRHEWVIMTKFTGVMRDSPYKTNNSSLGYMRRAV
                     EASLRRLNTDYIDLYQVHQWDPTTPLEETMAGLNDLVREGKVNYIGISNWPSWAVAEA
                     NTLARQHGWAPLVSSQPRYNVIDRTVEAEHIPACLRFGLGLIPWSPLAGGFLTGKHRR
                     GEEPAEGTRMANSAWARQVMTDRNWDRLERLEAFAAQRGITMTQLAIGWLA"
BASE COUNT          543 a         1098 c         1128 g          523 t
ORIGIN      
        1 ctaccgtctg cccgtagagg aactgcgcgc gcgtccagcc gaggtcatcg ctcatcggga
       61 cgaagaaggc gccagccacg tacgcctgga cgccggcggt cgtgaacgac gccacgaagg
      121 cggcgccgac gagacgccag ccgtagaaga agcgcggcct tgcgacggcg ctcgccgcgc
      181 cttgagccac ctgtggctcg acctcggtcg atccgatgct catgcgagcc ccccgctgcg
      241 cactatagcg caggggtgtt cgtgcgatac ggctccggcg gaggcacggc tcacagccgc
      301 acccgactac atctgatccc gaggaccctg gccaggccag tgcagagtgc cgttcgtcag
      361 ttcgatcggc tccccatcgt acgacgcgct caagagtcgc ccgccgcgct ccacgtcgac
      421 gtgggccggc cgcgtgggat tcttgaggcc gggcgactca ccaagcggaa taatctcggc
      481 atgcacgtga tcccgcgtga cctcgagcag gacgacggag cccaggcgga ccgtctggtt
      541 gtgcggcagg gtcaggctgc ccgagttgat gatgagggtg ttgtcctcgt attcgagccg
      601 ctcgtagtgg gtgtcgccag cgatcaggat gttgagctcg gtgtcgccgt acccgcgccg
      661 tttgaggtgg ccgactcgct cctcgccccg gtgacggtgg tcgaggacga tgtgcgccat
      721 ccccatccgc cagccttcac gctccagcag gacgacgtcc tcgaggcgcg ggtcgtcgaa
      781 ggtgtcgtgg ttgccgcggt tcacgatcag cggcccgaac tgctccatcc agtccaggac
      841 gagcgggccg gtgacgtctc ccgtgtggac gatgaggtcc accgacgcga ggaattcgcc
      901 cggcccgggg ccgaactcat cgagtggtct gccggtgtgc gtgtccgaga ggatgccaat
      961 gcgcatcagc cgacattaca ccattcatgg attgcgggcc gctggcctcg ttgggggcgc
     1021 gggagctgca ggtggcgtca gtgcacgacg ccgcccgccc gcaggccggc gatttcatcg
     1081 gcagtcagtc cgaactcgcc cagcacctcg tccgtgtgcc agccgagggg aggcgacggg
     1141 cgggtgctgc ccaccggcgt cttcgagagc tgcaccacgg ggccgaccat gcgctccggg
     1201 cccgtcagcg ggtggtcgaa gtccatcatg tagcccatcg cctcgacctg cgggtcgtag
     1261 gccagctcct cggggaagtt caccttcgag acgggcgcgc cgaggctgtc gaagcgctcg
     1321 atccactcgt ccatcgtgcg ggtaagcatg atgttgcgga tgcgttcccg cacctggtca
     1381 acaacgatgt cattcgcggg gtccagggca ttgaagtccg gcgaagcggt cgggtcgtcc
     1441 tcgaggccaa ttgcctggcg catctgctgc tggttgaggg gagtaagcgc gccgaggatg
     1501 agcgcaccgt ccttcacacg gtagccgccg taccagaggc ggaacgcctt gttgaggatg
     1561 cccgcctgcg cgcggattgc cagctgctcc tcgtaggact gtccctgagc tcgggcttcc
     1621 tggacgcggt cgaacatcgg gccgatcgcc atcgcgtcga acacgggcag cttcgcgacg
     1681 aacgaaccct gcagcaccag tccggactgt agcagcgagg tcgagacctt ctgcccctcg
     1741 ccggtcaggc tgcggtgaaa gagggccgcg cacgcgccga ccgcggcgga caccgccgcg
     1801 aggtaatcgg caggcgctgt ggccgtgatc atctgcggcg cgccccactc gtcgatcttg
     1861 gcgtcgccgg ccatcagccc cgagtaggcc tgcgccacga tgtccgagcc agagcggtcg
     1921 gcgcccggcc cttcggtgcc gaacccggtg ttctcgatgt agatcaggtc cggccggagc
     1981 gcgctgagcg tcgcgtagtc gagctgcagt ttcgccggca ccgagggccg tgcattgatg
     2041 atgaagatat cgaccgaggg catgaggcgg tggatgagcg ccttcccctc ggccttggtc
     2101 aggtcgacga cgaggctgcg cttgcctcga ttcagggagt ggtagccctt gctttcgccg
     2161 gggatgaacg cgccgttcac gcgtgtgctc tcgccgcccg gcggctcgac cttgattacg
     2221 tcggcaccga ggtccgcgag caccacgccg cagaacggcc cggcgacgac ctgagttacg
     2281 tcaagtacct tgatacccgc aagtggaccc ggcatgtcga gactcccctc ctgagcgtga
     2341 cgccgcgatt ataggggtga tgacgcgccc gccaagcccg ttgccacgtt gacggaggct
     2401 gcggccggga gtacgttccg ccggttgaca catacccgga gggggtactg agtcgctgcg
     2461 aggaccagcg gaagggaacg ggccatggaa atccgtcaga tgggacgctc ggggttgcgc
     2521 gtctcggtgg ttgcgctcgg gggcaacacc ttcgggccgg cggtagacgc cgcgcagacc
     2581 gcgaacatcc tcgcggccgc gcacgaccgc gggatcaacg ccgtcgacac cgccgacatc
     2641 tacaacaacg ggatgtccga ggagcacatc ggcaaggcag tggcgggcaa ccggcacgag
     2701 tgggtcatca tgaccaagtt caccggggtc atgcgggatt cgccgtacaa gaccaataac
     2761 tcttcgctcg gatacatgcg cagggccgtc gaggcgtccc tgcggcgcct gaacacggac
     2821 tacatcgacc tctaccaggt gcaccagtgg gacccgacga cgccactcga ggagacgatg
     2881 gcgggcctca acgacctcgt gcgcgagggc aaggtgaact acatcggcat ctcgaactgg
     2941 ccctcctggg cggtcgccga agcgaacacg ctcgcgcggc agcacggttg ggcgccgctg
     3001 gtctcctccc agccgcgcta caacgtgatt gaccgcacgg tcgaggcgga gcacatcccg
     3061 gcctgcctcc gcttcgggct gggactgatc ccgtggagcc ccctcgccgg cggcttcctc
     3121 accggcaaac atcggcgcgg cgaggagccc gccgagggca cgcgcatggc caacagcgcg
     3181 tgggcgcgcc aggtgatgac cgaccgcaac tgggaccgcc tcgaacggct cgaggcattc
     3241 gccgcgcagc gcggcatcac gatgacccag ctcgccatcg gctggctcgc gg
//
LOCUS       DAORPG010000152         3265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2747_length_3265_cov_3.206542, whole genome shotgun sequence.
ACCESSION   DAORPG010000152 DAORPG010000000
VERSION     DAORPG010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2747_length_3265_cov_3.206542"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /gene="treS"
                     /locus_tag="PLX85_04455"
     CDS             complement(<1..1272)
                     /gene="treS"
                     /locus_tag="PLX85_04455"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HRC62456.1"
                     /translation="MSPLEDDPLWYKHAVIYELPVRAFFDSNGDGIGDFRGLTSKLEY
                     LERLGVDCLWLLPFMQSPLRDGGYDISDYTAIHPTLGTMSDFTRFMREAHERGIRVIT
                     ELVVNHTSDQHPWFQRARRAPRGSAWRDMYVWSDTDQKWPETRIIFMDTETSNWAWDP
                     VAGQFYWHRFFSHQPDLNFDSPRARRAVENVMRFWYDRQVDGLRLDAIPYLFERDGTN
                     NENLPETHALLREWRNFVDERYPGRAFLAEANQWPADVVAYFGDGDECHMAYHFPLMP
                     RMFMALRQEDRHPIIDILGQTPEVPGNCQWCLFLRNHDELTLEMVSAEERDYMYSVYA
                     ADVQMRRNLGIGRRLSPLLSNNRRAIELLHCLLFTLPGTPVLYYGDEIGMGDNIYLGD
                     RDGVRTPMQWTADRNAGFSRANPQRLYLPVII"
     gene            complement(1269..3248)
                     /locus_tag="PLX85_04460"
     CDS             complement(1269..3248)
                     /locus_tag="PLX85_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HRC62457.1"
                     /translation="MLPPPYSHRPSRVVVDAVFPSVDGGRHPAKAIAGDPVFVRGHVF
                     ADGHDVLRAVARYRRLGEKRWSETPLTLATGDEWEGWFTAGAPGEMEFTVEGWVDRFA
                     TWRGDLEKRITAEWDVSAELLDGAALLRRLAGHAKGDTARGLKRFATRIEGARGSYTE
                     RVEAALDQLLAALAQAADPREGATNYRHLRLVVEPQRARFGAWYEFFPRSAGTDPTRS
                     ATFAEAAARLPDIAAMGFDVIYLPPIHPIGTTARKGRNNALVAQPDDPGSPWGIGSLE
                     GGHMAVHPDLGTLEDFDRFLEQAAGHGLDVALDLALQCSPDHPWVTEHPEWFRHRPDG
                     SIRYAENPPKQYQDIYPLNFETDDWAALWLEIRKVVDFWIGHGVHIFRVDNPHTKPFR
                     FWHWLIAGVHETHPEVIFLAEAFTRPKVQQALAKFGFSQSYTYFTWRNTKAQIEQLFT
                     ELTQRPLADYFRPNLFANTPDILHAYLQEGGKPAFLVRLVLAATLGPSYGIYSGFEFC
                     ENVAVEGTEEYLDSEKYEIKPRDWDAPGNIKPTITRLNFLRREYAALQFNHRVWFLPT
                     NDDHVVACIKRPPLGGPDVLVVVNLDVTSARDAWFGLPLESLGLPLEAPFLVEDALSG
                     VTQSWAGEWNHVRLDPEVMPAAVFILPERANEEPA"
BASE COUNT          529 a          989 c         1204 g          543 t
ORIGIN      
        1 gatgatgacc ggcaggtaga gcctctgcgg gttggcgcgg gagaagccgg cgttgcggtc
       61 ggcggtccac tgcatgggcg tgcggactcc gtcgcggtct ccgaggtaaa tgttgtcccc
      121 catcccgatc tcgtctccgt agtacaggac gggcgtgccg gggagcgtaa agagcaggca
      181 gtgcaggagt tcaatggcgc gacggttgtt gctgagcagc gggctcaggc ggcggccgat
      241 gccgagattg cgccgcatct gcacgtcggc cgcgtagacc gaatacatgt agtcgcgctc
      301 ctcggcggag accatctcaa gggtcagctc gtcgtggtta cgcaggaaga ggcaccactg
      361 gcagttcccg gggacctcgg gcgtctggcc gaggatgtcg atgatcgggt ggcggtcttc
      421 ctggcgcagc gccatgaaca tgcgcggcat gagcgggaag tggtaggcca tgtggcactc
      481 gtcgccatcg ccgaagtagg cgacgacgtc ggcaggccac tggttcgcct cggccaggaa
      541 ggcgcggccg gggtaacgct catcgacgaa gttgcgccac tcgcggagca gcgcgtgggt
      601 ctccgggagg ttttcgttgt tggtgccatc gcgctcgaag aggtagggga ttgcatcgag
      661 gcgaagcccg tcgacctggc ggtcgtacca gaagcgcatc acgttctcga ccgcacgacg
      721 ggcccgcggg ctgtcgaagt tcaagtccgg ctggtgcgag aagaagcgat gccagtagaa
      781 ctggccggcc accgggtccc aggcccagtt cgacgtttcc gtgtccatga agatgatgcg
      841 cgtctcgggc cacttctggt ccgtgtcgct ccacacgtac atgtcgcgcc aggccgagcc
      901 ccggggggcc cgccgcgcgc gctgaaacca ggggtgctgg tccgaggtgt ggttcacgac
      961 cagctcggtg atgacgcgga tgcctcgttc gtgggcttcg cgcatgaagc gcgtgaagtc
     1021 cgacatcgtc ccgagcgtcg ggtggatggc ggtgtagtcg gagatgtcgt agccgccgtc
     1081 gcggagcggc gactgcatga acggcaggag ccagaggcag tcgacgccga ggcgttcgag
     1141 gtactccagc ttcgaggtca ggcctcgaaa gtctccaatg ccatcgccgt tgctgtcgaa
     1201 gaaggcgcgg acgggaagtt cgtagatgac tgcgtgcttg taccagagcg ggtcgtcctc
     1261 gagcgggctc atgcgggttc ctcgttggcc ctctcgggca gaatgaagac ggcggcgggc
     1321 attacctctg ggtcgagccg cacgtggttc cattcgccgg cccaactctg cgtgaccccg
     1381 ctcagggcgt cctcgaccag gaacggcgcc tcgaggggga gaccgagcga ttcgagtggc
     1441 aggccgaacc aggcgtcgcg tgccgatgtc acatcgaggt tcacgactac caggacatcg
     1501 ggcccgccga gcgggggccg cttgatgcag gcaacgacgt ggtcgtcatt ggtcggcagg
     1561 aaccagacgc gatggttgaa ctggagcgcg gcgtactcgc ggcggaggaa attgagccgc
     1621 gtgatggtgg gcttgatatt gcccggcgcg tcccagtcgc gtggcttgat ctcgtacttc
     1681 tcggaatcga gatactcctc ggtgccctcg acggcgacgt tctcgcagaa ctcgaagcca
     1741 ctgtagatgc cgtaggacgg cccgagcgtg gcggcgagca cgaggcggac gaggaatgcc
     1801 ggcttgccgc cttcttgcag ataggcgtgc aggatgtcgg gcgtgttcgc gaagaggttc
     1861 ggccggaagt agtcggccag tggccgttgc gtcagctcgg tgaagagctg ctcgatctgc
     1921 gccttcgtgt tgcgccaggt gaagtaggta taagactggc tgaacccgaa cttggcgagg
     1981 gcctgctgca ccttcggacg ggtgaacgcc tcggcgagga agatgacctc gggatgcgtc
     2041 tcatggacgc ccgcgatcag ccagtgccag aaccggaacg gcttcgtgtg cgggttgtcg
     2101 acccggaaga tgtgcacccc gtggccgatc cagaagtcga ccaccttccg gatctcgagc
     2161 cacagcgccg cccagtcgtc ggtttcgaag ttgagcggat agatgtcctg gtactgcttc
     2221 gggggattct ccgcgtagcg gatgctgccg tcgggccggt ggcggaacca ctcgggatgc
     2281 tcggtgaccc aggggtggtc cggcgagcac tggagggcga ggtcgagcgc gacgtcgagg
     2341 ccgtgcccgg ccgcctgctc caagaagcgg tcaaagtcct cgagcgtccc gaggtcggga
     2401 tggacggcca tgtggccacc ctcgagggag ccgatgcccc agggtgaacc cggatcgtca
     2461 ggctgcgcca cgagcgcgtt gttgcggccc ttccgggcgg tcgtcccgat ggggtgaatc
     2521 ggtgggaggt agatcacgtc gaagcccatg gcggcgatgt ccggcagccg ggcggccgcc
     2581 tcggcgaagg tcgcgctgcg ggtcgggtcg gtaccggccg agcgcggaaa gaactcgtac
     2641 cacgcgccga agcgtgcacg ctgcggctcg acgacgagcc ggaggtggcg gtagttcgtg
     2701 gcgccctcac gcgggtcggc ggcctgggcg agcgcggcga gcagctggtc gagggcggcc
     2761 tcgacacgct ccgtgtatga gccgcgcgcg ccttcaatcc gggtcgcgaa gcgcttcaag
     2821 cctcgtgcgg tatcgccttt cgcgtgcccg gcgagacggc gcagcagggc cgcgccatcg
     2881 aggagttcgg cgctcacgtc ccactccgcc gtgatgcgct tctcgaggtc accgcgccag
     2941 gtcgcgaagc ggtcgaccca gccctcgacg gtgaactcca tctcaccggg tgcgccggcg
     3001 gtgaaccatc cctcccattc gtcgccggtc gccagtgtga gcggcgtctc ggaccagcgc
     3061 ttctcgccca gccgacgata gcgcgcgacg gcgcgcagca cgtcgtgccc atcggcgaag
     3121 acgtgcccgc ggacgaagac ggggtcgccg gcgatcgctt tcgccgggtg gcgcccgccg
     3181 tcgacgctcg ggaagaccgc atcgactacg actcgggacg gacgatggga gtacggcggg
     3241 ggcagcaggc gacggaggcg aggca
//
LOCUS       DAORPG010000153         3262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2755_length_3262_cov_3.774867, whole genome shotgun sequence.
ACCESSION   DAORPG010000153 DAORPG010000000
VERSION     DAORPG010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2755_length_3262_cov_3.774867"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..340)
                     /locus_tag="PLX85_04465"
     tRNA            complement(266..340)
                     /locus_tag="PLX85_04465"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(306..308),aa:Thr,seq:cgt)
     gene            complement(407..757)
                     /locus_tag="PLX85_04470"
     CDS             complement(407..757)
                     /locus_tag="PLX85_04470"
                     /inference="COORDINATES: protein motif:HMM:NF021119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HRC62458.1"
                     /translation="MSSLTNIPERATAASCFGAAATAGDRTVIPVAEVWYGLGFGWGG
                     GQSERDGVENEGSGGGGGGGSRVRGVAVIEVAPDGVRVHPIVDQTAVALAGIAFASAA
                     VAITARMLLKLIRG"
     gene            complement(783..1079)
                     /locus_tag="PLX85_04475"
     CDS             complement(783..1079)
                     /locus_tag="PLX85_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62459.1"
                     /translation="MQERDRHRPGNVEDTPLGRVGDRELRGVVERRELRLGPVVLGRV
                     RPRAVEVYEPGVGRRTVQVDAPVDPYLRALRWVLVMVVLAWVAGAVIGRTRTRK"
     gene            complement(1092..1808)
                     /locus_tag="PLX85_04480"
     CDS             complement(1092..1808)
                     /locus_tag="PLX85_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62460.1"
                     /translation="MQAFLVLLRYDLSQMGRSWIVRIWVFLLFIPAVFVLGVAANENE
                     LASESLAAYITAVMAPLSWLAVSIYSASAVSGEAGVATDGILSKSVTRTEYISAKVAA
                     RVGVTIIVYLMVMLPFTYLLRHYADPDTTGTGVFMGLLMVGTLLAFLAALGILLSTIF
                     RNVQIAVLCALMSVLLSGLALQFLELKWLSTTAVINGLPETFRGDTSMLTSIRVVVVF
                     IALTVAAVSSSLWLFRQKDL"
     gene            complement(1777..2880)
                     /locus_tag="PLX85_04485"
     CDS             complement(1777..2880)
                     /locus_tag="PLX85_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC62461.1"
                     /translation="MSVIETTDLTKIYHNGYIALNALSMRVDKGMVYGLVGPNGAGKS
                     TTFRVLLGLQKQTAGSVKVFGEEMHAERADLRRRIGFLPTSPSFPRNLTPIDYLKLVG
                     AITGMPRDRIKIRLAMLLRAVDLTSAAAQRIDGLSTGMLTRLGIAAALMNDPELLILD
                     EPTSGLDPTGRKQTIELIRDLSGRDRTIIIATHILSDVERICTDVGIISQGRLIYHGP
                     MSEMRRLARHGTISVEIEGNTAAFEQQIPLLDDIGDVTWERIGSEFRIKFVGAERLPV
                     YIQRVLDLIDRTGVELLHFNTGEDELEEAFLRRLEEDRVRGSLRSSWAATQRLSGDNR
                     LILPEPEYQNGALPSEVIEEEVVNAGVSGPPSL"
     gene            complement(3032..>3262)
                     /locus_tag="PLX85_04490"
     CDS             complement(3032..>3262)
                     /locus_tag="PLX85_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62462.1"
                     /translation="PKDGGYGLFVFGGGTNNDLVAASGCPKATATFWATNATGQFVTF
                     IPGTAVQAVNDSWNRLFPDGIPYNTALIGRCV"
BASE COUNT          595 a         1110 c         1045 g          512 t
ORIGIN      
        1 ccacgacctc ggtcagcgac tctgcggctc cggcatggag caacacgtcg gcgccggcct
       61 cgactgcagt gccggccgcg gccgcatcgc gcacctccgc gatgaccagc acctggcgca
      121 cgctgcgctc ggcggtccga gtcgccgtga acccaaaacc attagcctgc cggcgcgaag
      181 tctcgcgcag cctgtcacgc aacttgctca tggggtctcc gagcgccggg cctgctccgc
      241 ctgaacgggc ccgcgagatg aaccatggag ccggcggtgg gacttgaacc cacgacctgc
      301 tgtttacgaa acagctgctc tgccgctgag ctacaccggc gcgggtttcg agtatacgga
      361 acgccgcggg ctcggctcaa atcaccggac cggcccggca tcgctcttag ccacggatca
      421 gcttgagcaa catgcgcgcg gtaatcgcga cagcagccga ggcgaacgcg atcccggcga
      481 gcgccaccgc cgtttggtcg acgatcggat gcacacgcac gccgtccggc gccacctcaa
      541 tcacggcgac gcctcgcact cgagagccgc ctccacctcc cccgccgctg ccctcgttct
      601 ccacgccatc gcgttccgat tggccgccgc cccagccgaa ccccaacccg taccagacct
      661 cggccaccgg gatcacggtg cgatcgccgg ccgtggccgc cgcaccaaag cacgaggcgg
      721 ccgtcgcgcg ctcggggata ttcgtcagac tgctcaaccc ctggggtggc tccatccggg
      781 acctactttc gagtacgtgt ccgcccgatg accgctccgg cgacccaggc gaggacgacc
      841 atgaccagta cccagcgtag cgcgcgcaga tagggatcga cgggcgcatc gacctgcacg
      901 gtgcgtcggc cgacgcctgg ctcatacacc tcgacggctc gtgggcgcac tcgaccgagg
      961 accacgggcc caaggcggag ttcgcgccgc tcgacgacgc cgcgcagctc ccgatcgccc
     1021 acgcggccga gcggagtgtc ctcgacgttg cctggtcggt ggcggtcgcg ctcctgcatc
     1081 gttcgacgga gctacaggtc cttctgcctg aacagccaca gtgacgacga aacggccgcc
     1141 accgtcaggg caatgaacac caccacgacc cggatggacg tcagcatcga tgtatcgccc
     1201 cggaaggttt cgggcaggcc gttgatgacc gcggtagtgc tcagccactt cagctcgagg
     1261 aactgcagcg ccagcccgga cagcagcacg ctcatcaacg cgcagaggac tgcgatctgc
     1321 acgttgcgga agatcgtcga cagcaggatg cccagcgccg cgaggaacgc cagcaacgtg
     1381 cccaccatca gcaggcccat gaacacgccg gtgccagtgg tgtcagggtc cgcgtagtgc
     1441 cgcagcaggt aggtaaacgg cagcatcacc atcagataca cgatgatggt cacgcctaca
     1501 cgcgccgcca ccttggcgga gatgtactcg gtccgcgtga cggacttcga caggatgccg
     1561 tccgtcgcta cacccgcctc gccacttacc gccgatgcgg agtagatgga cacggcgagc
     1621 catgacagcg gcgccatcac cgcggtgata taggcggcca gcgactcgga agccagctca
     1681 ttctcgttcg cggcgacgcc gagtacgaag accgcgggga tgaacagcag gaagacccag
     1741 atgcggacga tccatgaccg tcccatctgg ctcaggtcat agcgaaggag gaccagaaac
     1801 gcctgcatta acgacctctt cttcgatcac ctcggacggc agcgcgccat tctggtattc
     1861 gggctcgggc aggatgagac ggttgtcgcc ggagaggcgc tgcgtcgccg cccaggagct
     1921 acgtagcgag ccacgtacgc ggtcctcctc gaggcggcgc aggaacgcct cctccagctc
     1981 gtcctcaccg gtgttgaagt gcaacagctc aactccggtg cggtcgatga ggtcgagtac
     2041 gcgctgaatg tagaccggca gccgctcggc gccgacgaac ttgatacgaa actcggagcc
     2101 aatgcgctcc caggtcacgt cgccgatgtc gtcgagcagt gggatctgct gctcgaaggc
     2161 ggcggtattg ccctcgatct cgaccgagat cgtgccgtgg cgcgccaggc ggcgcatctc
     2221 cgacatcggt ccgtggtaga tgaggcggcc ctgcgaaatg atgccgacgt ccgtgcagat
     2281 ccgctcgacg tccgaaagga tgtgcgtcgc gatgatgatc gtacggtcac ggcccgagag
     2341 gtcacgaatc aactcgatgg tctgcttgcg tccggtcggg tcgaggcccg aggtcggctc
     2401 gtcgaggatg agcagctcgg ggtcgttcat cagcgccgcg gcgatgccga gacgggtcaa
     2461 cataccggtc gacagcccgt cgatgcgttg cgcggccgcg ctcgtcaggt ctaccgcacg
     2521 caacagcatc gccaggcgaa tcttgatgcg gtcacgcggc atgcccgtga tcgccccgac
     2581 cagcttgaga tagtcgatgg gcgtcaggtt acgaggaaag ctcgggctgg tgggcaggaa
     2641 gccgatccgc cgtcgaaggt cggcgcgctc ggcgtgcatc tcctcaccaa acaccttcac
     2701 cgagccagcc gtctgcttct gcagtcccag caacacccgg aaggtcgtcg acttgcccgc
     2761 gccgttcggc cccacgaggc cgtacaccat ccccttgtcg acccgcatcg agagtgcgtt
     2821 cagggcgata tacccgttgt ggtagatctt cgtgagatcg gtcgtctcga tgacggacat
     2881 gggggaggct ccctcgtggg gtcgggcttg cccgggccgc ccgggcgcca tcccgagcgt
     2941 gggattcttt cggggcaacg atgatagcaa tccgcaacgg tcagcgaaac gacgaggctc
     3001 ctcgctgctc agtgagcgag acggtcgacg gtcagacgca gcggccgatc agggccgtgt
     3061 tgtaggggat cccatcgggg aagagcctgt tccaggagtc gttgacggcc tgcacggcgg
     3121 tccccggaat gaacgtcacg aactgcccgg tagcgttggt cgcccagaac gtcgccgtcg
     3181 ccttcggaca gccggacgcg gctacaaggt cgttgttcgt gccaccgccg aagacgaaga
     3241 gtccgtaccc gccgtccttt gg
//
LOCUS       DAORPG010000154         3247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2785_length_3247_cov_3.377506, whole genome shotgun sequence.
ACCESSION   DAORPG010000154 DAORPG010000000
VERSION     DAORPG010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2785_length_3247_cov_3.377506"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="PLX85_04495"
     CDS             complement(<1..1081)
                     /locus_tag="PLX85_04495"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HRC62463.1"
                     /translation="MQDFDSRQHVLQACRDHDVKFIRLWFTDILGMLKSVAITVEELE
                     HALEDGVTFDGASIEGFARTDEADMIMRPDPQTFQLLPWRPRERAVARMFCDIEGPDG
                     GPFEGDPRWVLKRTVERAAAAGFTCYVSPEIEYFYFRDAHSLEPLDSGGYFDLTPLDS
                     GSDLRRETVLTLEEMGIPVALSHHEGAPSQHEIDLRYTDALTMADSVMTYRLVVKEVA
                     MNHGVYATFMPKPLTDENGSGMHLQFSLFRGEENVFSGADTHSGLSTAGEQFMAGILR
                     HAPEMMLLTNQWVNSYRRLVPGFEAPTYATWSPRNHADLLRVPRHRPGEAAAARIEYR
                     APDAACNPYLVFAGILAAGLAGIEAG"
     gene            complement(1185..2600)
                     /gene="glnA"
                     /locus_tag="PLX85_04500"
     CDS             complement(1185..2600)
                     /gene="glnA"
                     /locus_tag="PLX85_04500"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRC62464.1"
                     /translation="MATLQEVLDLASSRSVRRVDLKVADLLGRWQHFTLPAHRLNADL
                     IADGQGFDGSSFRGFQQIHESDMLLMPDLDTAVIDPAYDTPTLSLICDVADPLTRQHY
                     SRDPRAIAKKAEEYLKTTGIADTAYFGPELEFFLFDDVRFQQGQNSAFYFVDSNEGSW
                     NSGREEGGNLSFKIAGKEGYSPAPPFDTQTEIRNEFVEALEGIGIPMELHHHEVATAG
                     QGELAMRFGTLTTQADRSQWYKYVVRNVSRKHGKVATFMAKPIYGDNGTGMHTHQSLW
                     KNGAPAFYDSNGYAGLSQVARWYIGGLLKHAHAVLAFAAPTTNSYKRLVPHYEAPVNL
                     AYSMRNRSASIRIPTYSMSANAKRIEFRPADATCNPYLAFAAMLMAGLDGIQKQTDPG
                     DPLDRNIYELPPDEAAKVPTVPGSLEQALDALEADHDFLLQGGVFTQDVIDTWLEYKR
                     AEAQEVRLRPTPYEYELYFAG"
     gene            complement(2806..>3247)
                     /locus_tag="PLX85_04505"
     CDS             complement(2806..>3247)
                     /locus_tag="PLX85_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC62465.1"
                     /translation="GVRIDDFQMPPLDIEEFRTRVGRALWEHTGADEGSLLRRRGLVI
                     DLERYTVSVDGEVVDLTYKEYELLRFLATNAGKPFTREALLNRVWGYDYYGGSRTVDV
                     HIRRIRAKIERHEAYIDTVRNVGYRFIDDAPTGARLDSLGAEGL"
BASE COUNT          497 a         1047 c         1117 g          586 t
ORIGIN      
        1 ccccggcctc gatcccggcg agcccggcag cgaggatgcc cgcgaagacg aggtacgggt
       61 tgcacgccgc gtccggtgcc cggtattcga tgcgcgccgc ggctgcctcg cccggccggt
      121 ggcgcgggac ccgcaacagg tcggcgtggt tgcgcggcga ccaggtggcg tacgttggcg
      181 cctcgaagcc cggtacgaga cgccgatacg agttcaccca ctggttcgtg agcagcatca
      241 tctccggcgc gtggcgcagg atgcccgcca tgaactgctc cccggccgtc gagaggccgg
      301 aatgcgtgtc ggcgccgctg aagacgttct cttcgcctcg gaagagcgag aactggaggt
      361 gcatccctga gccgttctcg tcggtgagcg gcttcggcat gaacgtggcg tagacgccgt
      421 ggttcatggc cacttccttc acgacgaggc ggtaggtcat cacggagtcg gccatcgtga
      481 gcgcgtctgt gtagcgcagg tcgatctcgt gctggctcgg cgctccctcg tggtggctga
      541 gcgccaccgg aatgcccatc tcctcaaggg tgagcaccgt ctcgcgacgc aggtcggagc
      601 cactgtcgag tggcgtgagg tcgaagtacc cgccgctgtc cagcggttcg agggagtgcg
      661 cgtcccggaa gtagaagtac tcgatttccg gtgacacgta gcacgtgaac ccggccgcag
      721 ccgcgcgctc cacggtccgc ttcaagaccc agcgcgggtc gccttcgaac gggccgccgt
      781 ccgggccctc aatgtcgcag aacatgcggg cgacggcccg ctcgcgaggc cgccacggca
      841 gcagctgaaa cgtctgtggg tcgggccgca tgatcatgtc ggcctcgtcg gtgcgcgcga
      901 acccctcgat cgaggctccg tcgaacgtca ccccgtcctc gagggcgtgt tccagctcct
      961 ctaccgtgat cgcgacggac ttcagcatgc cgaggatgtc cgtgaaccag agacggatga
     1021 acttcacatc gtggtcgcgg cacgcctgca gcacgtgctg gcgtgagtcg aagtcctgca
     1081 tcgctcgctt cctctcggta ggccctcgct ccggggacaa aggtcgagga gaaggcagcg
     1141 gccccggttg cccggggccg ctggaatcgt tgccgcgcgg gcggttagcc ggcgaagtac
     1201 agctcgtact cgtacggcgt cgggcgaagg cggacctcct gcgcctcggc acgcttgtac
     1261 tccagccagg tgtcgatgac gtcctgggtg aagacgccgc cctgcagcag gaagtcgtga
     1321 tccgcctcga gggcgtccag cgcctgctcg agcgagcccg ggacggtcgg gaccttggcg
     1381 gcctcgtcgg gcggcagctc gtagatgttg cggtccagcg ggtcacccgg gtccgtctgc
     1441 ttctggatgc cgtcgaggcc cgccatcagc atcgcggcga acgcgaggta cgggttgcac
     1501 gtcgcgtccg ccgggcggaa ctcgatgcgc tttgcgttcg ccgacatcga gtacgtcggg
     1561 atgcggatcg aggcggagcg gttacgcatc gagtacgcga ggttcactgg cgcctcgtag
     1621 tgcgggacca gtcgcttgta cgagttggtc gtgggggccg cgaacgccag cacggcgtgg
     1681 gcgtgcttca agaggccgcc gatgtaccag cgcgccacct gcgacagacc ggcgtacccg
     1741 ttcgagtcgt agaacgcggg cgcgccgttc ttccacagcg actggtgcgt gtgcataccg
     1801 gtgccgttgt cgccgtagat cggcttcgcc atgaacgtgg cgaccttgcc gtgcttccgg
     1861 gagacgttgc ggacgacgta cttgtaccac tgcgagcggt ctgcctgcgt ggtcagcgtg
     1921 ccgaagcgca tcgccagctc gccctggccc gcggtcgcca cctcgtggtg gtgcagctcc
     1981 atcggaatgc cgatgccctc gagcgcctcg acgaactcgt tgcggatttc ggtttgcgtg
     2041 tcaaagggcg gcgccggcga gtagccttcc ttgcccgcga tcttgaagga gaggttgccg
     2101 ccctcctcgc ggccgctgtt ccacgagccc tcgttcgagt ccacgaagta gaacgcggag
     2161 ttctggccct gctggaagcg cacgtcgtcg aagaggaaga actcgagctc ggggccgaag
     2221 tacgccgtgt cggcgatccc ggtcgtcttg aggtactcct cggccttctt cgcgatcgcg
     2281 cgcgggtcgc ggctgtagtg ctgccgggtg agcgggtcgg cgacgtcgca aatcaggctg
     2341 agcgttggcg tgtcgtacgc ggggtcgatc accgccgtgt cgaggtccgg catcagcagc
     2401 atgtccgact cgtggatctg ctgaaagcca cggaagctcg agccgtcgaa gccctggccg
     2461 tcggcgatca ggtccgcgtt tagacggtgg gccggcagcg tgaaatgctg ccatcgaccg
     2521 aggaggtcgg ccaccttcag gtcgactcgg cgcacgctcc tcgagctggc caggtcgagc
     2581 acttcttgca atgtcgccac gttcgcctct ccctctaccg gcacccgaag ggtgcgttga
     2641 tgctcgcgag cggtaccccg gcctctgtgg ccgtacacgc tgcacgaatt ctgaaccgtg
     2701 cggggtcaga ggaaaaggta gagccgagcg gttactctcc gattactccc ctgttacgaa
     2761 catgtttcgt cgctgttcgc gacaatgaag ccgaagcgac ggcttctaca acccctccgc
     2821 gccgagtgag tccagccgcg cgcccgtcgg cgcatcgtcg atgaagcgat agccgacgtt
     2881 gcgcacggta tcgatgtagg cctcgtggcg ttcgatcttc gctcggatgc gccggatgtg
     2941 gacgtctacg gtgcgggacc ctccgtagta gtcgtagccc cagacgcgat tcaggagtgc
     3001 ttcacgcgtg aacggcttgc ccgcattcgt ggcgaggaac cggagcagtt cgtattcctt
     3061 gtaggtgagg tcgacgacct cgccatcgac gctcaccgta taccgttcga ggtcgatcac
     3121 gagaccgcgt cggcgcagca ggctgccctc gtcggcgccg gtgtgctccc agagggcgcg
     3181 ccccacccgg gtgcggaact cctcgatgtc gagtggcggc atctgaaagt cgtcgatccg
     3241 gacgccg
//
LOCUS       DAORPG010000155         3244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2791_length_3244_cov_3.221700, whole genome shotgun sequence.
ACCESSION   DAORPG010000155 DAORPG010000000
VERSION     DAORPG010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2791_length_3244_cov_3.221700"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="PLX85_04510"
     CDS             complement(<1..129)
                     /locus_tag="PLX85_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW motif-containing protein"
                     /protein_id="HRC62466.1"
                     /translation="MAAKVRRNDTVEVTTGKDRGKRGEVRRVLPAEQRVVVAGLNMV"
     gene            complement(132..503)
                     /gene="rplN"
                     /locus_tag="PLX85_04515"
     CDS             complement(132..503)
                     /gene="rplN"
                     /locus_tag="PLX85_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRC62467.1"
                     /translation="MIQQQSRLKVADNSGARELLCIRVLGGSGRRYARVGDTIIASVK
                     EAQPNSNVKKGDVVRAVVVRTAHTYQRPDGSAIRFDENAAVLLADKAGNPRGTRIFGP
                     VARELRDREYMRIVSLAPEVL"
     gene            complement(656..934)
                     /gene="rpsQ"
                     /locus_tag="PLX85_04520"
                     /pseudo
     CDS             complement(656..934)
                     /gene="rpsQ"
                     /locus_tag="PLX85_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
     gene            complement(956..1201)
                     /gene="rpmC"
                     /locus_tag="PLX85_04525"
     CDS             complement(956..1201)
                     /gene="rpmC"
                     /locus_tag="PLX85_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRC62468.1"
                     /translation="MPSKKETVELRGRDDAQLQADLEAAHHALFNLRFQAATRQLADV
                     SQIAKARRRVARARTLLRERAILAEVEAARSAAAQSE"
     gene            complement(1201..1620)
                     /gene="rplP"
                     /locus_tag="PLX85_04530"
     CDS             complement(1201..1620)
                     /gene="rplP"
                     /locus_tag="PLX85_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRC62469.1"
                     /translation="MLMPRRVKYRKQHRGRRAGAAMSGNTVAFGEWGLQAQTTAWVDS
                     RQIESARRAITGSLRRGGKVWIRIFPDKPVTKKPVETRMGKGKGPPDHWVAVVKRGRV
                     MFEVAGVSEVAVREAFRRAHHKLPVDTKVIGREEGGA"
     gene            complement(1625..2296)
                     /gene="rpsC"
                     /locus_tag="PLX85_04535"
     CDS             complement(1625..2296)
                     /gene="rpsC"
                     /locus_tag="PLX85_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRC62470.1"
                     /translation="MGRKVHPYGFRVGITKDWQSRWFSEREYATLLAEDMRLRRLVMR
                     MLPDAGISAITIDRNANQLSLTVRTAKPGIVIGRGGQNVEQLRGALEGATDKRVRLNI
                     EEIRVPELDAQLVARSVADQLERRVAFRRAMKQAVQRTMQRGALGCRVKISGRLGGSE
                     MSRSEQEMQGRVPLHTLRADIDFGQAEAHTTFGRIGVKAWIYKGDIAPDQTPFALAGA
                     EGGTE"
     gene            complement(2297..2638)
                     /gene="rplV"
                     /locus_tag="PLX85_04540"
     CDS             complement(2297..2638)
                     /gene="rplV"
                     /locus_tag="PLX85_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRC62471.1"
                     /translation="MEVRALAADQPVSARKVRLVLDRIRGLGVAEALTILEFLPQPSA
                     RIVWKTVKSAAANAENNYNLDPDRLTVKRASAGESRTLKRFRARSRGRAAPVLKRTSH
                     IEIVVEGDEGF"
     gene            complement(2638..2922)
                     /gene="rpsS"
                     /locus_tag="PLX85_04545"
     CDS             complement(2638..2922)
                     /gene="rpsS"
                     /locus_tag="PLX85_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRC62472.1"
                     /translation="MSRSTKKGPFIEESLMRKVEAVQRSGAKQAIRTWSRASTIMPEM
                     VGLTIAVHDGRRHVPVFCTENMVGHKLGEFAFTRTFRGHKGRSETSSRSR"
     gene            complement(2926..>3244)
                     /gene="rplB"
                     /locus_tag="PLX85_04550"
     CDS             complement(2926..>3244)
                     /gene="rplB"
                     /locus_tag="PLX85_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009663069.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRC62473.1"
                     /translation="LRLPSGEMRYVPEACLATVGVVGNAEHALLKLGKAGRTRHRGRR
                     PQVRGSVMNPVDHPHGGGEGKASVGMPGPKTPWGKPALGYRTRNNKRTDRDIQRRRGG
                     GRR"
BASE COUNT          440 a         1064 c         1202 g          538 t
ORIGIN      
        1 gaccatgttg aggccggcga cgacgacgcg ctgctcggcg gggaggacgc gacgaacctc
       61 gccccgcttc ccacggtcct tgccagtggt cacctcgacg gtgtcgttgc gccggacttt
      121 ggcggccatg gctacagcac ctccggggcg agcgagacga tgcgcatgta ctcgcggtcg
      181 cgcagctcgc gagcgacggg gccgaagatg cgcgtgccgc gcgggttgcc ggccttgtcg
      241 gcgaggagca cggcggcgtt ctcgtcgaag cggatagcgg agccgtccgg gcgctggtag
      301 gtgtgcgcgg tgcgcacgac gaccgcgcgg acgacgtcac ccttcttgac gttgctgttg
      361 ggctgggcct ctttgacgct cgcgatgatg gtgtcaccga cgcgggcgta acgacggccg
      421 gagcccccga gcacgcgaat gcagagcagt tcgcgcgcgc cggagttgtc ggcgaccttg
      481 agccggcttt gctgctgaat cattgctgtt ccgactccgt ctcggtctcg ggagccgcct
      541 ccgcagcggg ctcctcggcg gccggagccg gagccgctga ctcagcggtg tgggcagcgg
      601 cgcgctggac ctcgctgacg agagaggcgt cgatggcctc ggcgcggacc tgggcgacct
      661 cgcgctgctg gacgatctcg acgagccgcc aacgcttggt cttgctgatg ggccggcatt
      721 cctcgatgcg gacgacgtca ccgacgctgg cggcgttcag cgggtcgtgc acggggtagc
      781 gccgggtacg ccggattacc ttgcggtaga ggcggtgggg cgcggcgcgc tcgatggcga
      841 cgatgatcgt ctggtcgccg cggtcggaga ccacggtgcc ggtacgagcc ttgcggttga
      901 cgacccgccc ctcggtggtt gccgcctcga ccatacgaaa ctcctcgtta cccgtttact
      961 cggactgcgc ggcagcggag cgggcggcct cgacttcagc gaggatggcc cgctcgcgca
     1021 gcagcgtcct ggcgcgtgcg acgcggcgac gcgccttggc gatctggctc acgtccgcca
     1081 gctgacgcgt ggcggcctgg aagcgcaggt tgaacagcgc gtgatgcgcc gcttcaaggt
     1141 cggcctgcag ctgggcgtca tcacgcccac gcagctcgac ggtctccttc tttgacggca
     1201 tcaggcaccg ccctcttcgc ggccgatcac tttcgtgtcg accggcagct tgtggtgcgc
     1261 gcggcggaag gcctcgcgca cggcgacctc ggagacgccg gcgacctcga acatgacgcg
     1321 gccgcgcttg acgaccgcga cccagtggtc cgggggccct ttgcccttac ccatacgggt
     1381 ctcgaccggc ttcttggtga ccggcttgtc cgggaagatg cgaatccaca cctttccgcc
     1441 acggcgcaac gaaccagtga tcgcgcgacg ggcggactcg atctggcggg agtcgaccca
     1501 ggcggtggtc tgggcctgca ggccccactc gccgaaggcg acggtgttgc cgctcatcgc
     1561 ggcgccggcg cggcggccgc gatgttgctt gcggtacttg acgcgacggg gcatcagcat
     1621 ggcgctactc cgtcccgccc tcggcgccag cgagcgcgaa cggggtctgg tcgggggcga
     1681 tgtcgccctt gtagatccag gccttcacgc cgatgcgtcc gaaggtggtg tgcgcctcgg
     1741 cctgcccgaa gtcgatgtcg gcgcgaaggg tgtgcagcgg cacgcggccc tgcatttcct
     1801 gctccgaccg cgacatctcg gaaccgccga ggcgaccgct gatcttgacg cggcagccga
     1861 gggcgccgcg ctgcatggtg cgctggaccg cctgcttcat ggcgcggcgg aaggcgacgc
     1921 ggcgctcgag ctggtcggcg acggagcggg cgacgagctg ggcgtcgagc tcagggacgc
     1981 gaatctcctc gatattgagg cggacgcgct tgtcggtggc gccttcgagg gcgccgcgga
     2041 gctgctcgac gttctggccg ccacggccga tgacgatgcc gggcttggcg gtgcggaccg
     2101 tgaggctgag ttggttcgcg ttgcggtcga tggtgatggc ggagatgccg gcgtccggca
     2161 gcatgcgcat cacgaggcgg cgcagacgca tgtcctccgc aaggagggtg gcgtactcac
     2221 gctcgctgaa ccagcgagac tgccagtcct tggtgatccc gacgcggaag ccgtaggggt
     2281 gaactttacg ccccatttag aacccctcgt ctccttcgac cacgatctcg atgtgcgacg
     2341 tgcgcttgag aaccggggcc gcgcggccgc gcgagcgagc gcggaagcgc ttgagcgtgc
     2401 gcgactcccc ggcgctggcc cgcttcacgg tgagccggtc cgggtccagg ttgtagttgt
     2461 tctccgcgtt ggcggcggcg gacttgaccg tcttccagac gatgcgcgcc gacggctgcg
     2521 ggaggaactc aaggatagtg agggcctcag ccacgcccag gcctcggatg cggtcgagga
     2581 cgagccgcac cttgcgggcg gagacgggct gatcggccgc gagagcacgt acttccatca
     2641 gcgcgacctc gaggacgtct cggagcggcc cttgtggccg cggaaggtgc gggtgaaggc
     2701 gaactcgccg agcttgtggc cgaccatgtt ctcagtgcag aagaccggca cgtggcggcg
     2761 gccatcgtgg acggcgatgg tgaggcccac catctccggc atgatcgtcg aggcgcgcga
     2821 ccaggtgcgg atggcctgct tggcgccgga gcgctgcacg gcctcgacct tgcgcatgag
     2881 gctttcctcg atgaaggggc cctttttggt cgatcgtgac atcggttacc tccgcccgcc
     2941 gccacgccgg cgctggatat cgcggtcggt gcgcttgttg ttcctggtgc ggtagccgag
     3001 ggcgggcttg ccccacgggg tcttcggccc gggcataccg acggacgcct tgccctcgcc
     3061 accaccgtgc gggtggtcga cggggttcat gacggaaccg cggacctgcg ggcgccgccc
     3121 gcggtggcgg gtgcggccgg ccttgccgag cttgagcagc gcgtgctccg cgttgccgac
     3181 gacaccgact gtggccaggc aggcctcggg cacgtagcgc atctccccgg aggggagacg
     3241 cagc
//
LOCUS       DAORPG010000156         3242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2798_length_3242_cov_2.680891, whole genome shotgun sequence.
ACCESSION   DAORPG010000156 DAORPG010000000
VERSION     DAORPG010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2798_length_3242_cov_2.680891"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PLX85_04555"
     CDS             <1..242
                     /locus_tag="PLX85_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62474.1"
                     /translation="AVYYTLGWCREFAANALDKPKRSRPHQAVGQDASTLYAMAKAAF
                     LRALQLEPEEGMRGDIEDMLDVIANATGEPWREGE"
     gene            242..1090
                     /locus_tag="PLX85_04560"
     CDS             242..1090
                     /locus_tag="PLX85_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC62475.1"
                     /translation="MPHLELEDGFRLYYESHGEGPAVLFAHGAGGNAISWWQQVPAFR
                     GRYRVITFDHRAFGRSPDIEGGPGRTAFGADAVALLDHLGVGAVHVVAHSMGARTAMA
                     LMRHDESRIRSLTLSGSNAGCVDDRLRARKAELEASGVLSGSLLSRALAADFVERAPE
                     LAHLYRQVRAVNPPRPADFLAPTPEMRNYRGSTAGRINDSGVPVLWVVGEHDRVVPAE
                     LVRISHELTPGSRYVEVVDAGHSAYFERAEAWNAAVRSFIDAVEDGVAGTRGTGDGRA
                     GAWTSS"
     gene            1075..1872
                     /locus_tag="PLX85_04565"
     CDS             1075..1872
                     /locus_tag="PLX85_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC62476.1"
                     /translation="MDLELRGRRAIVTGGSRGIGKAIALELAREGVDIAICARDRATL
                     DAAAADIRALGVRAAGIEVDAGNRASVDAAVEEAVAQLGGVDILVNSAGQPGGLATGP
                     LPTVTDEDVLEDFNAKTFGTLRFCRAVAPHLQQQGWGRIINIGGLSGRQPGTYSTGMR
                     NVAITHLTRTLSLELAPSGITVNIVHPGMTRTEWAAGRWAEAARRQGLPPEEGERIAS
                     ERALMKRVVDTSEIAYVVAFLASPKASAVSGEVIGVGGGEPGRSLFL"
     gene            1896..2489
                     /locus_tag="PLX85_04570"
     CDS             1896..2489
                     /locus_tag="PLX85_04570"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRC62477.1"
                     /translation="MAGESIIGRRIAVVGSSGSGKSTVASLLAERLALPFVELDALYW
                     KPGWVESEHEEFVGRVQSETAGEGWVVAGNYSRAWPVYWPRAETVVWLDLPLRVCLGR
                     LVRRSWRRSRERELLWGTNTERFWKHLKLWNANDSLLTWTVLARGPLERRVMREAGPD
                     GMATGRVVRLRSAADVEEFVQLVAPEVATDAALAGVR"
     gene            2491..>3242
                     /locus_tag="PLX85_04575"
     CDS             2491..>3242
                     /locus_tag="PLX85_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC62478.1"
                     /translation="MLTLTDISVRFGGQLLYEDVNWQLSPRGHYGLVGANGSGKSTLL
                     RVMTGELAPTTGSVSQIRGLNIGTLGQDHFRFDDRTPIDVVRMGRPRLWQALEERAGL
                     LLEVVEGGAAQQGERLAEIEAEIGGLGGYQSEAEAAALLVGLGVPHERHERPMRELSG
                     GFRLRVLLAQTLFFAPDLLLLDEPTNHLDIASIRWLEGYLREFRGAFVVISHDRHFLN
                     AICDQIADVDYQEVRLYAGNYDAFEAAKTLAST"
BASE COUNT          455 a          948 c         1376 g          463 t
ORIGIN      
        1 ccgcggtgta ctacacgttg gggtggtgcc gggagttcgc cgcgaacgcc ctcgacaagc
       61 cgaaacgcag ccggccgcac caggccgtag gccaggacgc ctcgacgctc tacgcgatgg
      121 caaaggcggc gttcctgcgg gcgttgcagc tcgagccgga ggaagggatg cggggcgaca
      181 tcgaggacat gctggacgtg atcgcgaatg ccacgggcga gccctggcgc gagggcgagt
      241 agtgccgcac ctcgagctgg aggacgggtt ccggctgtac tacgagtcgc acggcgaggg
      301 gccggcggtg ctgttcgcgc acggcgcggg cggcaatgcc atctcgtggt ggcagcaagt
      361 gccggcgttc cgcgggcggt accgggtcat caccttcgac caccgggcgt tcgggcggtc
      421 gcccgacatc gagggcggac ccgggcggac ggcgttcggc gcggacgcgg tcgcgctgct
      481 cgaccacctc ggggtgggag cggtgcacgt ggtggcgcac tcgatgggcg cgcgcaccgc
      541 gatggcgctg atgcggcacg acgagtcgcg gatccgatcg ctcacactgt cggggtcgaa
      601 tgcggggtgc gtggacgatc ggctgcgcgc gcggaaggcg gagctggagg cgtcgggggt
      661 cctgagcggg tcgctgctgt cgcgcgcgct cgcggcggac ttcgtggaac gggcgcccga
      721 gctggcgcac ctgtaccggc aggtgcgagc ggtgaatccg ccgcggccgg cggacttcct
      781 cgcgcccacg cccgagatgc ggaactatcg gggttcgacg gcggggcgca tcaacgacag
      841 cggcgtgccg gtgctgtggg tggtcggcga acacgaccgc gtggtgcccg cggagctggt
      901 gcgcatctcg cacgagctga cgccggggtc gcggtacgtg gaggtggtgg atgccgggca
      961 ctcggcgtac ttcgagcggg cggaggcgtg gaacgcggcg gtgcgttcgt tcattgacgc
     1021 ggtcgaggat ggcgtcgcgg ggacgcgggg caccggggac ggaagggcgg gggcatggac
     1081 ctcgagttga gggggcggcg ggcgatcgtc acgggcggaa gccgtgggat cgggaaggcg
     1141 atcgcgctgg agctggcgcg cgagggcgtg gacatcgcga tctgcgctcg cgaccgggcg
     1201 acgctcgacg cggcggccgc tgacatccgc gcgctcgggg tgcgggcggc cggtatcgag
     1261 gtggacgcgg gcaaccgcgc gagcgtcgac gcggccgtcg aagaagcggt tgcgcagctg
     1321 ggcggggtgg acatcctcgt gaatagcgcg gggcagcccg ggggtctcgc gaccgggccg
     1381 ctgccgacgg tgaccgacga ggacgtgctg gaggacttca acgcgaagac cttcgggacg
     1441 ctgcggttct gccgggcggt ggcgccgcac ctgcagcagc agggctgggg ccgcatcatc
     1501 aacatcgggg ggctgtcggg gcggcagccg gggacgtaca gcacggggat gcggaacgtg
     1561 gcgatcactc acctgacgcg gacgctgtcg ctcgagctgg cgccatccgg gatcacggta
     1621 aacatcgtgc atccggggat gacgcggacg gagtgggcgg cgggccgctg ggcggaggcg
     1681 gcacgacggc agggactgcc gccggaggag ggcgaacgga tcgcctcgga gcgggcgctg
     1741 atgaagcggg tggtggacac gagcgagatc gcgtacgtgg tggcattcct cgcgtcgccc
     1801 aaggcgagcg cggtgtcggg cgaagtgatc ggggtcgggg gcggcgagcc ggggcgctcg
     1861 ctcttcctgt aggacggcgc tgctcgagga tggcgatggc gggcgaatcg atcatcggaa
     1921 ggcggatcgc ggttgtcggc agctcgggga gcggcaagtc gacggtggct tcgttgctgg
     1981 cggagcggct cgcgctgccg ttcgtggagc tggatgcgct gtactggaag cccggatggg
     2041 tggagtcgga gcacgaagag ttcgtgggcc gggtgcaatc ggagaccgcc ggcgagggat
     2101 gggtggtcgc cgggaactac tcgcgagcgt ggccggtgta ctggccgcgc gccgagacgg
     2161 tggtctggct cgacctgccg ctgcgcgtgt gcctcggccg cctcgtgcgg cgttcgtggc
     2221 ggcggtcgcg ggagcgcgag ttgctgtggg gtacgaacac ggagcggttc tggaagcacc
     2281 tgaagctctg gaacgcgaac gactcgctcc ttacgtggac ggtcctggcg cggggccccc
     2341 tcgaacggag ggtgatgcgc gaggcggggc ccgatgggat ggccaccgga cgcgtcgtgc
     2401 ggctgcgctc ggcggcggac gtcgaggagt tcgtgcagct cgtggcgccc gaggtcgcga
     2461 cggacgcggc gctggcgggc gtgcgctgag atgctgacgc ttaccgacat ctcggtccgc
     2521 ttcggcgggc agctgctgta cgaggacgtc aactggcagc tgagccctcg agggcactac
     2581 ggcctcgtgg gggcgaacgg cagcgggaaa tcgacgctgc tgcgggtcat gactggcgag
     2641 ctggcgccga cgaccgggtc ggtgagccag atccgcgggc tgaacatcgg gacgctgggg
     2701 caggaccact tccggttcga cgaccggacg ccgatcgacg ttgtgcgcat ggggcggccc
     2761 cggctgtggc aggcgctgga ggagcgggcg gggttgctcc tcgaagtggt cgaggggggc
     2821 gcggcgcagc agggcgagcg gctggcggag atcgaggcgg agatcggggg gctgggcggg
     2881 taccagtccg aggccgaggc agccgcgctg ctcgtggggc tgggggtgcc gcacgagcgg
     2941 catgagcggc cgatgcggga gctatcgggc gggttccggc tgcgggtgtt gctggcgcag
     3001 acgctgttct tcgcgccgga cctgctgttg ctcgacgagc cgacgaacca cctcgatatc
     3061 gcctcgatcc gctggctaga ggggtacctg cgggagttcc gcggggcgtt cgtcgtgatc
     3121 tcgcacgacc ggcacttcct gaacgcgatc tgcgaccaga tcgcggacgt ggactaccaa
     3181 gaggtgcggc tgtacgccgg gaactacgac gcgttcgagg cggcgaagac cctggcttcc
     3241 ac
//
LOCUS       DAORPG010000157         3240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2799_length_3240_cov_3.345997, whole genome shotgun sequence.
ACCESSION   DAORPG010000157 DAORPG010000000
VERSION     DAORPG010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2799_length_3240_cov_3.345997"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PLX85_04580"
     CDS             <1..344
                     /locus_tag="PLX85_04580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC62479.1"
                     /translation="DQLHVVTVAVDPAEQGRGIAQRLLLEAHRLAVEAELATIALEVR
                     VTNERARRLYEWFGFTQAGTLRRYYSDNGEDAVVMLTPPLTDDAFEAHVRGLRATLLD
                     RFGDWQAAPGG"
     gene            complement(360..1247)
                     /locus_tag="PLX85_04585"
     CDS             complement(360..1247)
                     /locus_tag="PLX85_04585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRC62480.1"
                     /translation="MTGQHADEHGGGDARRLGVALILVLVTAAGELWGSVVTGSLALL
                     SDMVHVSTDFVALALALVSVRVAARPHTLQWTFGFHRIEILAAALNGLSLLVVSAFIV
                     REALARIGDPGDVHAAGLTAIATIGLGSNAVTAVVLGHSHSMNMRAARLHVLSDLGGS
                     VVAVIAGVAIGITGATRIDPVLSLVIVALVVVGAVRLLREAVEVLMDRVPRGIDLAEV
                     EGALLTVPGVTGVHDMHCWTIASGFIAFAAHLQLAPGADPQMSIEHAATILEGRYGIA
                     HTTLQPEQAPSPRLVEFSA"
     gene            complement(1278..2318)
                     /locus_tag="PLX85_04590"
     CDS             complement(1278..2318)
                     /locus_tag="PLX85_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC62481.1"
                     /translation="MPGDPPRRKKRTHHFEGPLGWLRKPTAKPSAAEGKEVVEGRATV
                     INEHLIQHIVSVKLFTRSFPTSNVWVLHDRGEAALIDAGFGDETSAQVRIDYLTKELS
                     NLDFKYIPITHHHFDHSSGGRRLRQALRAEVAMHPIDEVLLHTPTESNEDLPDDAEID
                     KRARIWRAEALETPVDVPLADGESVRVGGLTVRAVHTPGHTAGHNCYFVEELGILFTG
                     DNILGIGTSAIAPPPAGDMEQYIQSLLRMRELQSKLFAPGHGPVVTATAAKVQELIDH
                     RAARDAQILALVDRGYTNDRQIRNALYPELQKGLRRAARGQLRAHLARLVGQGKVTVE
                     EEGKDWKVALTR"
     gene            2478..3140
                     /locus_tag="PLX85_04595"
     CDS             2478..3140
                     /locus_tag="PLX85_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62482.1"
                     /translation="MPSEAYREARATAKQARATAKEARKRWLHLRPWYFKKRFILGIP
                     ILLAMAAVVAVITISIERESSPATEVSGHSADVSATGSATAASNASEPGRLGALVRIG
                     SIDLTVLAFEPIDSRTFSPANDANYRIRFHITNARGPASENYRLVDAAFQLIDGAGSA
                     HDDWRAVCQNCPDALGSGVSLTLNGSVDGAVYFRIPAGQTPVELRYQSPNSTNSGRIA
                     LR"
BASE COUNT          480 a         1280 c          993 g          487 t
ORIGIN      
        1 tcgaccagct ccatgtcgtt acggtcgccg tcgacccggc cgagcagggc cgaggcatcg
       61 cccagcgcct gctcctcgag gcgcaccgtc tcgccgtgga ggccgagctc gccacgatcg
      121 ccctcgaggt ccgcgtcacc aacgagcgcg cccgccgcct ctacgaatgg ttcggcttca
      181 cccaggccgg caccctgcgc cgctactact cggacaatgg cgaggacgcc gtcgtgatgc
      241 tcaccccgcc cctcacagac gacgcgttcg aggcacacgt gcgcggcctt cgcgccaccc
      301 tcctcgaccg cttcggcgac tggcaggccg cccccggggg ctaacgcccg aaccgtggct
      361 caggcgctga actccacgag ccgcggcgac ggcgcttgct cgggctgcaa tgtcgtatgg
      421 gcgatcccgt aacggccttc gaggatcgtc gccgcatgct cgatgctcat ctggggatcc
      481 gcgccgggcg cgagctgcag gtgcgcggcg aaggcgatga acccgctcgc gatcgtccag
      541 cagtgcatgt cgtgcacgcc ggtaacgccc ggcactgtca ggagcgcccc ctctacctcg
      601 gcgaggtcga tgccgcgcgg gacacggtcc atgagcacct cgactgcctc ccgcagcaat
      661 cggacggccc ccaccaccac gagcgcgacg atgacgagcg aaaggacggg gtcgatgcgc
      721 gtggcgcccg tgatcccaat cgcgacgccg gcgatcacgg cgaccacgga cccgccgagg
      781 tccgacaaca catgcagccg cgccgcgcgc atgttcatcg agtgcgagtg ccccagcacg
      841 acggccgtca ccgcgttgct tccgaggccg atcgtggcga tcgcggtcag ccccgcggcg
      901 tgtacgtcgc ccggatcgcc gatgcgtgcg agggcttcgc gcacgatgaa cgccgacacg
      961 acgaggagtg acagcccgtt cagcgccgcc gcgagaatct cgatgcgatg gaagccgaac
     1021 gtccactgca gggtgtgagg ccgggccgcg acgcgcacgc ttaccagcgc gagcgcaagc
     1081 gccacaaaat ccgtggacac atggaccatg tccgagagca gtgccagcga accggtgacg
     1141 acgctgcccc acagctcgcc cgccgccgtg accagcacga ggatcagcgc gactccgagc
     1201 cgccgggcgt ccccgcctcc gtgctcgtcg gcgtgctggc ccgtcatcag gctaggatac
     1261 gcggcgcctc gcgccggcta ccgcgtcagc gcgaccttcc agtccttgcc ctcttcctcg
     1321 accgtcacct tcccctgccc caccagcctc gcgaggtgcg cgcgcagctg cccgcgtgcc
     1381 gcccgccgca ggcccttctg cagctccggg tacagcgcgt tgcggatctg ccggtcgttc
     1441 gtgtacccac ggtcgaccag cgcgaggatc tgcgcgtccc gcgccgcccg atggtcgatc
     1501 agttcctgca ctttcgccgc cgtcgcagtg accacgggcc cgtgccccgg cgcgaacagc
     1561 ttcgactgca gctcgcgcat ccgcagcagg ctctggatgt actgctccat gtcgcccgcc
     1621 ggcggcggcg cgatcgccga ggtcccgata ccgaggatgt tgtccccggt gaacaggatc
     1681 cccagctcct ccacgaagta gcagttatgc cctgcggtgt gccccggtgt gtgcacggcc
     1741 cgcaccgtca gcccgccgac acgcactgac tccccgtccg cgagcggcac gtccaccggc
     1801 gtctcgagcg cctccgcgcg ccagatgcgc gcccgcttgt cgatctccgc gtcgtccggc
     1861 aggtcctcgt tgctctccgt cggcgtgtgc agcagcacct cgtcgatcgg gtgcatcgcc
     1921 acctcggcgc gcagcgcctg ccgcagccgc cgcccgcccg aggagtgatc gaagtggtgg
     1981 tgcgtgatcg ggatgtactt gaagtcgagg ttgctcaact ccttcgtcag gtagtcgatg
     2041 cgtacctgcg ccgacgtctc gtccccgaac cccgcgtcga tgagcgccgc ctcaccccgg
     2101 tcgtgcagca cccacacgtt cgaggtggga aacgaccgcg tgaacagctt caccgacacg
     2161 atgtgctgga tcaggtgctc gttgatgacg gtcgcccgcc cctcgacgac ctctttgccc
     2221 tccgccgccg agggcttcgc cgtcggcttc cgcagccagc ccagcggtcc ctcgaagtga
     2281 tgcgtgcgct tcttcctgcg cggcgggtcc cccggcatct gcggctcctc gtctgttcgc
     2341 aacgtctgcg aggactatac ccgcggccgc actcacgtcg gcgctccgcc cctccccacg
     2401 gttggcacgc cctgaacccc cgccgtacga tccgccgggc ggcgtcgacc gcgccgctcg
     2461 gttggaaagg cgcgccgatg ccctcggaag cctatcgcga ggcacgtgcc accgcgaagc
     2521 aggcgcgcgc cacggcgaag gaagcacgca agcgctggct gcacttgcgc ccctggtact
     2581 tcaagaagcg cttcatcctc ggcatcccga tcctgctcgc gatggccgcc gtcgtcgccg
     2641 tcatcaccat cagcatcgaa cgtgagtcgt cgcccgccac cgaggtcagc ggtcactccg
     2701 ccgacgtctc cgcgacgggc agcgccaccg ctgcctcgaa tgcctccgag ccgggacgtc
     2761 tcggcgctct cgtgcgcatc ggcagcatcg acctcaccgt gctggccttc gagcccatcg
     2821 acagccgcac cttcagcccg gccaacgatg ccaactaccg catccggttc cacatcacca
     2881 acgcgcgcgg ccccgccagc gagaactatc gcctcgtcga cgccgccttc cagctcatcg
     2941 atggcgccgg ctccgcccac gacgactggc gcgccgtctg ccagaactgc ccggacgccc
     3001 tcggctccgg cgtctcgctc accctcaacg gctcggtcga tggcgccgtc tacttccgca
     3061 tccccgcggg ccagaccccc gtcgagctcc gctaccagtc ccccaactcc acgaacagcg
     3121 gccgcatcgc cctccgctga cccgaccgcg cgaaccgctt gccttccgcc gagcccccct
     3181 cgatgctgct cccatgacca ccgacgccgg ccagcagtgg aacgccagcg cgtatgggca
//
LOCUS       DAORPG010000158         3223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2841_length_3223_cov_3.665720, whole genome shotgun sequence.
ACCESSION   DAORPG010000158 DAORPG010000000
VERSION     DAORPG010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2841_length_3223_cov_3.665720"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLX85_04600"
     CDS             <1..261
                     /locus_tag="PLX85_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62483.1"
                     /translation="VLGAMKRRYEVRALGMSSPPPLMTPEDALADSRWRYWVEPGSAG
                     DERLGQLLVERYGQPDGRVQLPPRRNYSAVISWEPAPFEATR"
     gene            complement(265..1632)
                     /locus_tag="PLX85_04605"
     CDS             complement(265..1632)
                     /locus_tag="PLX85_04605"
                     /inference="COORDINATES: protein motif:HMM:NF019149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRC62484.1"
                     /translation="MQAVCYLRATPSTPLADQQRLFLDYCDRHGLEAAQVYQEDPGAA
                     NAPQFRRMLREQGGTRRGFSAIVVASLEVLGPTAREQARRYLQLQALALPLRIADGRD
                     PDQALLSTWLARDPRERRRDQVREGMRRRALRGEVLGRAPYGYRVVDRHLHIDPVEGD
                     VVREIFRRYLEEGEGVRVIARRLNEAGIPTRRGGTWSMVSVRGVLKNPVYTGTYRRLG
                     VVVATEHDALVTRGRFYAAQQRLAQRRTSGGPQARSQYLLSGLARCGYCGNSLIGVRR
                     VRAARRPEDKPVVYTYYQCESRTNQSRCAYHTRRAPELEGIVRERLRQGPPSGVAATE
                     EPAALDEELTRLRGRRDGIRRQLDALLERHARGEWTMERLRAEASTLALEDLAAEERA
                     ESLVERRTAGVDGRRREATLARARARLAREWDDLEFQQRQDLLREVATAVIVTDDDVR
                     VDFAG"
     gene            1723..2271
                     /locus_tag="PLX85_04610"
     CDS             1723..2271
                     /locus_tag="PLX85_04610"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRC62485.1"
                     /translation="MQFNVSTLLQEPVGSTRLAEVEDEPVAVPAAGYASTVSGRARLM
                     RTQRGVLVHADLTTHPALECARCLTSFQGEIRLLIDEEFVPLRDPVTGEAIEADPDEF
                     RIDGRNHLDLSEAVRQYEQAALPIQPVCREDCAGLCPVCGQNLNERRCDCEQPEPEGA
                     WAGLAALAERLRTEEHDGSPEA"
     gene            2252..2437
                     /gene="rpmF"
                     /locus_tag="PLX85_04615"
     CDS             2252..2437
                     /gene="rpmF"
                     /locus_tag="PLX85_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRC62486.1"
                     /translation="MAVPKRRTPPAKQGQRRSHHHVVAAQIARCPQCRSPRVNHRICP
                     VCGSYKGRQVLGSSDSR"
     gene            2522..>3223
                     /locus_tag="PLX85_04620"
     CDS             2522..>3223
                     /locus_tag="PLX85_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HRC62487.1"
                     /translation="MSSVSIPSLPRSGSTAWLFPGQGAQAVGMGRDLYADVPAARAVF
                     DAADEALGFPLSTLCFEGPEDELTRTINTQPALVTHAIAALVAAIDCGTLHEGPAYLA
                     GHSLGEYAALIAAGAVSFEDGIRLVRERGRLMQEACDAQAGTMAAILGLERDQVASIC
                     GEHGASLCNENAPGNISIGGTLEAVRAASEAATSAGASRVVPLNVAGAFHTPLMQSAA
                     DGMRIVLGSTTFADPQ"
BASE COUNT          501 a         1137 c         1091 g          494 t
ORIGIN      
        1 gtcctgggcg cgatgaaacg ccgctacgag gtgcgagcct tggggatgtc gagcccgcct
       61 ccactgatga cgccggagga cgcactcgcc gactcgcgtt ggcgctactg ggtcgagcca
      121 ggatcggcgg gcgacgagcg cctcggtcag ttgctcgtcg agcggtacgg gcagcccgat
      181 ggccgcgtac agctgccgcc gaggcgaaac tactcggcgg tgatttcctg ggaacctgcg
      241 cctttcgagg ccactcgata acgatcagcc ggcgaagtcg acgcgcacgt cgtcgtcggt
      301 cacgatcaca gctgtcgcga cctcgcgcag gagatcctgg cgctgctgaa attcaaggtc
      361 gtcccattcc cgagcaaggc gcgcgcgggc acgcgcaagc gtggcctccc gccgccgacc
      421 atcgacgccg gccgtgcgcc gctcgacgag tgactccgct cgttcctcgg ccgcgaggtc
      481 ctcgagcgcc aatgtcgagg cctcggcccg caggcgctcc atcgtccatt caccgcgtgc
      541 atgccgctcg agcaaggcat cgagctgtcg ccggatgccg tcacgccgtc cccgcaggcg
      601 ggtcagttcc tcgtcgaggg cggcgggttc ttccgtcgcc gcgaccccgg agggcgggcc
      661 ctgccgcagg cgctcacgca cgatcccttc gagttcgggg gcgcgacgcg tgtggtaggc
      721 gcaccgactc tggttcgtgc gtgactcaca ctggtaatag gtgtagacca ccggcttgtc
      781 ctcgggacga cgtgcggcac ggactcggcg cacgccaatc agcgagttcc cgcagtagcc
      841 gcaacgggcc aggccgctga gcaggtactg actccgagcc tggggcccgc cgctcgtgcg
      901 ccgctgcgcg aggcgctgct gcgcagcgta gaatcgcccg cgcgtgacga gggcgtcgtg
      961 ctcagtcgcc acaacgacac ccagcctgcg gtaggtcccc gtgtacaccg gattcttcag
     1021 taccccgcgc acgctgacca tgctccaggt gccgccccgc cgggtgggaa tccccgcctc
     1081 gttcaggcgg cgggcgatga ccctcacgcc ctccccctcc tcgaggtagc ggcgaaagat
     1141 ctcgcgcacc acgtcgcctt cgactggatc gatgtggagg tggcggtcaa cgacccggta
     1201 gccgtacggc gcccgaccga gcacctcccc gcgcaacgcc cggcgacgca tgccctcacg
     1261 cacctggtcg cggcggcgtt cgcgagggtc acgtgcgagc cacgtcgaga gcagcgcctg
     1321 gtcgggatcc ctgccgtcgg caatgcgcag cggtagtgcc agcgcctgga gctgcaggta
     1381 gcggcgcgcc tgctcgcggg cggtcgggcc gagtacctcc agcgacgcca cgacgatagc
     1441 ggagaagccg cggcgcgtgc cgccttgctc ccgcagcata cggcgaaact gcggcgcgtt
     1501 cgccgcgcca gggtcttcct ggtagacctg cgccgcctcc aacccgtggc ggtcacaata
     1561 gtccaggaac agccgctgct ggtcggccag cggcgtcgac ggagtcgcac gcagataaca
     1621 gaccgcctgc atcgcaagcg accttcctcc cgccgcaggg cagtatacgc gccacgcccg
     1681 ccggccttct ggtgttacga tcgaggggtc ccggaggaac cagtgcaatt caacgtatcc
     1741 accctgctcc aagagccggt cgggagcacc cgtctcgctg aggtcgagga cgagccggtc
     1801 gccgtgcccg cggcgggata cgcctcgact gtcagcggcc gcgcgcgcct catgcgcacg
     1861 cagcgcggcg tgctcgtcca tgccgatctc accacccacc cggcgctgga gtgtgcccgc
     1921 tgcctcacct cgttccaggg cgagatccgg ctcctgatcg atgaggagtt cgtaccgctg
     1981 cgcgacccgg tgacaggaga ggcgatcgag gccgacccgg acgagtttcg catcgacggc
     2041 cggaaccacc tcgacctcag cgaggcggtg aggcaatatg agcaggctgc gttgccgatc
     2101 cagcccgtct gtcgcgagga ttgcgcgggg ctgtgtcccg tctgcggaca gaatctgaac
     2161 gaacgacgct gcgattgtga acagcccgag ccggaaggcg cctgggcagg actggctgct
     2221 ctggcggagc ggctgcgaac ggaggaacac gatggcagtc ccgaagcgta ggactccgcc
     2281 cgcgaagcaa ggccagcgcc gcagtcacca ccatgtcgtc gccgcgcaga ttgcccgctg
     2341 cccgcagtgc cggtccccgc gtgtgaacca ccgcatttgc ccggtgtgtg gcagctataa
     2401 gggccgccag gtgctgggtt cgagcgactc gcgctagcgc tcgtcgcgca ctcctctgga
     2461 ctgagccgag ccggtacact cccccggacg ccactcttca cggaacgacg ggggtcttcg
     2521 catgtccagc gtctccatcc cttctcttcc acgttctggt tctacggcat ggctgtttcc
     2581 agggcagggc gcgcaggccg tcgggatggg ccgcgacctg tacgctgacg tcccggccgc
     2641 ccgggcggtg ttcgacgccg cagacgaggc gctcggcttc ccgttgtcga cgttgtgttt
     2701 cgaaggcccc gaggacgagc tcacgcggac gatcaatacg cagcccgcgc tcgtcacaca
     2761 cgccatcgcg gcgctggtgg cggccatcga ctgtggcaca ttgcacgagg ggcccgcgta
     2821 tctcgcgggg cattcgctcg gtgagtacgc cgcattgatc gccgcaggcg cggtgagctt
     2881 cgaggacggc atccgcctcg tccgggagcg cgggcggctc atgcaggaag cgtgcgacgc
     2941 gcaagccgga acgatggcag ccatcctagg cctcgaacga gatcaggtcg cttccatctg
     3001 cggcgagcac ggcgcatcgc tgtgcaacga gaacgcaccg gggaacatct cgatcggcgg
     3061 cacgctggaa gcggtgcgcg cggcttccga ggcagcaacg tcggcgggag cgagccgcgt
     3121 cgtccccctc aatgtggcgg gggccttcca cacgccgctc atgcagagcg ccgccgacgg
     3181 catgcggatt gtgctcggct caaccacctt cgccgatccg cag
//
LOCUS       DAORPG010000159         3216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2855_length_3216_cov_3.375514, whole genome shotgun sequence.
ACCESSION   DAORPG010000159 DAORPG010000000
VERSION     DAORPG010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2855_length_3216_cov_3.375514"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..181
                     /locus_tag="PLX85_04625"
                     /pseudo
     CDS             1..181
                     /locus_tag="PLX85_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676429.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
     gene            327..1481
                     /locus_tag="PLX85_04630"
     CDS             327..1481
                     /locus_tag="PLX85_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRC62488.1"
                     /translation="MQAARRLPVTIVRGEGTRVWDSDGREYLDFVAGIAVVSLGHADP
                     QLAEVIAKQAHTLLTVSNIFYTEPQIELAELLVRHSALDRVFFVNSGAEANESAIKLA
                     RKWGNIHKQGAFEIISTNNSFHGRTMATVSATGTPRYREPFGPQLPGFVFVDYNDLDA
                     LKAATNERTCAVLLEPVQGEGGINVPAADYLRQVRAWCDEQQLLLILDEVQTGMGRTG
                     TLWAYEQAGIEPDIMTSAKGLGGGVPIGAVLAKEHAAVFEPGDHGNTFGGNPLVTAAG
                     AHVMHRLVEGGVLENVRERAAQLAQRLHGLEDRFEVVKGVRGVGLLQGLELHREIAVD
                     VVGAGIKLGILLNPVRPDIVRMMPPLTITAEEVDRGVDLLEEALRQVTAG"
     gene            1899..3143
                     /locus_tag="PLX85_04635"
     CDS             1899..3143
                     /locus_tag="PLX85_04635"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRC62489.1"
                     /translation="MGETIVLAYSGGLDTSVATKWLQEARGYEVVCLMVDLGRLPDVD
                     SARERGLKAGALAVDVVDAKEDFLRFFVYPALGAGAVYEGSYPLATALGRPLIAKLLV
                     DKAREVGATAVAHGCTGKGNDQVRFDVSIRALGPDLKVVGTARENLMNRDEAIAYAER
                     HGIPIKQTVASPYSTDENLWGRSVEAGTLEDPWMEPPEEVYAWTKPVRATPEEPAYVT
                     IRFDHGAPVALDGEELRAVELVERLNALAGEHGVGRIDMVEDRLVGIKSREIYESPAA
                     VTLLEAHRSLEALTLTKLQLRFKERVAQEYADLIYNGLWFTAHHQDLAAYVQSTQRHV
                     TGEVRMRLHRGTATAVGRQSERSLYSHALATYSEGDQFDQSAALGFIKLHGLPVEVQA
                     RRQLMLDAKDDDPLRLGTVGEE"
BASE COUNT          490 a          924 c         1314 g          488 t
ORIGIN      
        1 gccgggcttc gtcgacgcgc atgtgcacat cggcttcggc cagcaagggc gccacggccc
       61 gctggcgccg accgactacg tctacaagct ctggctcgcg catggcgtga cctcggtgcg
      121 cgaggtcggc agcatgaacg ggctggcgtg gaccatcgac caggcgcgcc aggcctccga
      181 tgccgcacaa caggacctcg accggctgcg gcagctgctg aaccagatcg aagcgcagtc
      241 gcagcgctag actccaacga gccgcgagca cagatgcaga ggagcgaacc gtgagtgaca
      301 ttcccgcgcg cgaggccgag gtctacatgc aggccgcgcg ccggctgccc gtgacgatcg
      361 tgcgcgggga ggggacgcgc gtctgggaca gtgacggcag ggagtacctc gacttcgtgg
      421 cggggatcgc ggtggtgtcg ctggggcatg cggacccaca gctcgcggag gtgatcgcga
      481 agcaggcgca cacgctgctg acggtctcga acatcttcta cacggagccg cagatcgagc
      541 tggcggaact cctcgtgcgg cattccgcac tggaccgcgt gttcttcgtg aactcgggcg
      601 cggaggcgaa cgagagcgcg atcaagctgg cgcggaagtg ggggaacatc cacaagcagg
      661 gggcgttcga gatcatctcg acgaacaatt cgttccacgg gcggacgatg gcgacggtgt
      721 cggcgacagg gacgcctcgc taccgggagc cgttcgggcc gcagctgccg gggttcgtgt
      781 tcgtggacta caacgacctc gatgcactga aggccgcgac gaacgagcgc acctgcgcgg
      841 tgctcctcga gccagtgcag ggggagggcg gcatcaatgt gccggcggcc gactacctgc
      901 ggcaggtgcg ggcgtggtgc gacgagcagc agctcctgtt gatcctcgat gaagtgcaga
      961 cggggatggg gcgcacgggg acgctgtggg cctacgagca ggcgggcatc gagccggaca
     1021 tcatgacctc ggcgaagggg ctggggggcg gcgtgccgat cggggcggtg ctggcgaagg
     1081 agcacgcggc agtcttcgag ccgggcgacc atgggaacac gtttgggggg aatcccctcg
     1141 tgacggccgc gggcgcacac gtgatgcacc ggctggtcga gggcggggtg ctggagaacg
     1201 tgcgggagcg ggcggcgcag ctggcgcagc ggctgcacgg tctcgaggac cgcttcgagg
     1261 tagtgaaggg cgtgcgcggt gtggggctgc tgcagggcct cgagctgcac cgcgagatcg
     1321 cggtggatgt ggtgggggcg ggcatcaagc tggggattct gctgaacccg gtgcgaccgg
     1381 acatcgtgcg gatgatgccg ccgctgacga tcaccgcgga ggaggtcgac cggggggtgg
     1441 atctgctcga ggaggcgttg cggcaggtga cggcggggta gtacctcggg ggggcgaccg
     1501 aggcctcgtt cccgctcggc agcggtttct cggatcctcc acactctccc ccgcaagcgg
     1561 ggcggggctc ggggccggcg ttggggtgtc tgtcttcgat gccggttgcg tcggcgccgg
     1621 gtcgggtgct ccgtcgcccc ctcagttccc ctctcccgcg ttgcgggcga ggcggagggc
     1681 ggccggctgc gccggccggg aggagacggc ccgctgcgtg ggccggcagt gtttttcgga
     1741 aggtcccacc cgtccgccct ggaacggggg cttcggtcgg cgtgtcccgg tcacatcgtg
     1801 aggagggtag cggcgagggc tcttatactc aggcgcgtcg ccgtttgacc ggtgtggcgg
     1861 gcgactccct cgtgctttgg tgagtggtgg agcgggtcat gggtgagacg atcgtgctgg
     1921 cgtactccgg ggggctggac acctcggtgg ctacgaagtg gctgcaggaa gcgcgcggct
     1981 acgaggtggt gtgcctgatg gtggacctcg gacggctgcc ggatgtggat tcggcgcgcg
     2041 agcgggggct gaaggcgggg gcgctggccg tggatgtggt cgacgccaag gaggatttcc
     2101 tgcgtttctt cgtgtacccg gcgctcggcg cgggtgcggt gtacgagggc tcgtacccgc
     2161 tcgcgacggc cctcggacgg ccgttgatcg cgaagttgct cgttgacaag gcgcgcgagg
     2221 tgggcgcgac ggcggtggca cacgggtgca cgggcaaggg caacgaccag gtgcggttcg
     2281 atgtgtcgat ccgggcactg gggccggacc tgaaggtggt ggggacggcg cgcgagaacc
     2341 tgatgaaccg cgacgaggca atcgcgtacg cggaacgcca cgggatcccg atcaagcaga
     2401 cggtggcttc gccgtactcg acggacgaga acctgtgggg gaggtcggtc gaggcgggga
     2461 cgctcgagga cccgtggatg gagccgcccg aggaggtgta tgcctggacg aagccggtgc
     2521 gggcgacgcc ggaggagccg gcgtacgtga cgatccgatt cgaccacggg gccccggtgg
     2581 ccctcgacgg ggaggagttg cgtgcggtcg agctggtgga gcggctgaac gcgctggcgg
     2641 gtgagcacgg ggtggggcgg atcgatatgg tcgaggaccg cctcgtgggg attaagtctc
     2701 gcgagatcta cgagtcgccg gcggccgtga cgttgctgga ggcgcatcgg tcgctggagg
     2761 cgctgacgct gacgaagttg caactgcgct tcaaggagcg ggtggcgcag gagtatgcgg
     2821 acctgatcta caacgggctg tggttcacgg cgcaccacca ggacctcgca gcctacgtgc
     2881 agtcgacgca gcggcatgtg acgggcgagg tgcggatgcg gctgcatcga gggacggcga
     2941 cggcggtcgg gcggcagtcg gagcggagtc tgtacagcca cgcgctggcg acgtacagcg
     3001 agggcgacca gttcgatcag tcggccgcgc tggggttcat caagctgcat gggctcccgg
     3061 tggaggtgca ggcgcgtcgg cagctgatgc tggacgcgaa ggacgatgac ccgttgcgcc
     3121 tcgggacggt gggggaggag tagctcccca cgtagtccag gcggagccgg aggcgcgccg
     3181 cttcgcggcg cgctgaggac ggccgctgcg ctgccg
//
LOCUS       DAORPG010000160         3205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2874_length_3205_cov_3.927937, whole genome shotgun sequence.
ACCESSION   DAORPG010000160 DAORPG010000000
VERSION     DAORPG010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2874_length_3205_cov_3.927937"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1421
                     /locus_tag="PLX85_04640"
     CDS             117..1421
                     /locus_tag="PLX85_04640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC62490.1"
                     /translation="MRWFSFAHREPWHAGEQEFPRYRWVIIGQVIAQQQAEPFIFAML
                     GILLPAMQDDLGFSSVQAGWLGTARSAGNLLVFGASLYLVRFSPIKTFNAFILVLAGA
                     LFVGLLAPNFWVLLLSLAVYSVGVSWGQIPMNMVRLQWIPPREGATVMGLLLSANTIT
                     QMAGLILLPIAVHHVSWRVIFAGNCLVLVACAIGWLLTAYERVAPSYAAALPGGRGLG
                     SAKTVLRRREFYLLGFATLGGATTFMTFMLFLPTYYTRERDISLQTAGFITAVVQLGG
                     LAVNLAAGMISDRLGRRKPLIWPSGLVLPFLWFLMLAPLPPLGLGMVGFVLGAFAWMP
                     FPIIQTIPLEIPGLSPSDRAVGQALQFTLQTSGQLVAPIAVGAAAAATGSYHTALMPL
                     IVLPVAFAVCMLFFPETGPRGRAARPAEARATTSTEVVSRGE"
     gene            complement(1506..2579)
                     /locus_tag="PLX85_04645"
     CDS             complement(1506..2579)
                     /locus_tag="PLX85_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRC62491.1"
                     /translation="MTVTQALMLDEQKLNEFIGKAVGDFGTTLSTALVVIGDQLGLYR
                     ALAARPLTSAELSEDTQTAERYVREWLVNQAAGGYLEYDPLSGRYSLPPEHAIALTQE
                     DSPAYVVGGYQVVSSLVLAFPRILQAFRDGSGMLWGEHDENLFEGTERFFKPGYLANL
                     VDSWLPALDGIDAKLRQGGTVADVGCGLGASTIIMAQAYPNSRFVGYDNHAPSIEAAR
                     EAAARAGVADRVAFEVASAADYPAAGRYDLIAFFDCLHDMGDPSGAMRHALEALAPDG
                     AVMIVEPMAGDRIEDNLNPVGRVFSGASTLVCTPNAVASGGEALGTLAPEAAIADVVR
                     GAGFSSFRRATETPFNRIFEAKR"
     gene            complement(2648..2821)
                     /locus_tag="PLX85_04650"
     CDS             complement(2648..2821)
                     /locus_tag="PLX85_04650"
                     /inference="COORDINATES: protein motif:HMM:NF018096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HRC62492.1"
                     /translation="MAYSIRCRDAGVECPGAFTTETPQEVIKHAQMHLSEAHPGKTVR
                     DEDLRQLIQTTVA"
     gene            complement(2869..3027)
                     /locus_tag="PLX85_04655"
     CDS             complement(2869..3027)
                     /locus_tag="PLX85_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62493.1"
                     /translation="MARKLVYRCYCGFAAEGEEDELVVVLTSHAFDVHQMTVSREQVL
                     AMAVPVAE"
BASE COUNT          461 a         1088 c         1082 g          574 t
ORIGIN      
        1 cgcgagagcg gccccgtccg ttgtcgctcc ctcgccgctg acccccgcgc ccgctacgct
       61 cacgcggacg cgtcgaggcg gcatccgcct cgtccgacgg gcggcaggca gcgaccatgc
      121 gatggttctc gttcgcgcac cgcgagccgt ggcacgcggg ggagcaggag ttcccgcgct
      181 accgctgggt gatcatcggg caggtgatcg cgcagcaaca ggcggagccg ttcatcttcg
      241 cgatgctcgg catcctgctg cccgcgatgc aggacgacct ggggttcagc agcgtgcagg
      301 cgggctggct ggggacggcg cgctcggcgg ggaacctgct cgtgttcggg gcgagcctct
      361 acctcgtgcg gttcagcccg atcaagacgt tcaacgcgtt catcctcgtg ctcgctggcg
      421 cactgttcgt cgggctgctg gcgccgaact tctgggtgct gctgctcagc ctggccgtgt
      481 actcggtcgg ggtgtcgtgg ggccagatcc ccatgaacat ggtgcgcctg cagtggattc
      541 cgccgcgcga gggggcgacg gtgatgggcc tgctgctctc cgcgaacacg attacgcaga
      601 tggcgggcct gatcctgctt ccgatcgcgg tgcaccacgt cagctggcgg gtgatcttcg
      661 cgggaaactg cctcgtgctg gtcgcctgcg cgatcggctg gttgctcacc gcctacgaac
      721 gggtcgcgcc gagttacgcc gccgcgcttc ccggcggccg cggccttggc tcggcgaaga
      781 cggtgttgcg ccggcgggag ttctacttgc tgggtttcgc gacgcttggc ggcgcgacca
      841 ccttcatgac tttcatgctc ttcctgccga cctactacac gcgcgagcgc gacatctcgt
      901 tgcagacggc ggggttcatc accgcggtcg tgcagctggg cgggctcgcg gtcaacctgg
      961 cggcggggat gatctccgac cggttggggc ggcggaagcc gctgatctgg ccatccggtc
     1021 tcgtgttgcc gttcctgtgg ttcctgatgc tcgcgccgct gccgccgctc ggcctcggga
     1081 tggtcggatt cgtgctcggg gcgttcgcgt ggatgccgtt tccgatcatc cagaccatcc
     1141 ccctcgagat cccgggactg agcccttcgg accgggcggt cggtcaggca ctgcagttca
     1201 cgctacagac gtcggggcag ctcgtggcgc cgatcgccgt cggcgccgcg gccgccgcga
     1261 cgggctcgta tcacaccgca ctgatgccgc tgatcgtgct gcccgtcgcc ttcgcggtgt
     1321 gcatgctctt tttccccgag acggggccac gcggccgtgc cgcacgcccc gccgaggcgc
     1381 gagctacgac atcgacagag gttgtcagca ggggcgagtg atcgaggagt cagcgcacga
     1441 actcccacaa catcaccgca gccggggagg aggcgcgccc cgcctcctcc ccgggttgcc
     1501 ttcggctagc gcttcgcctc gaagatgcgg ttgaacgggg tctcggtggc ccgacggaag
     1561 ctcgagaagc cggccccgcg cacgacgtcg gcgatcgccg cctcgggcgc cagagtgccg
     1621 agcgcctcgc cgcccgaggc gaccgcgttc ggggtacaga ccagggtcga ggcgccggag
     1681 aagacccgac caacgggatt caggttgtcc tcgatgcggt cacccgccat cggttcgacg
     1741 atcatgaccg cgccatcggg cgcgagcgcc tcaagcgcgt gacgcatggc gcccgacggg
     1801 tcgcccatgt cgtgcaggca atcgaagaac gcgatgaggt cgtaacggcc ggcagcggga
     1861 taatcggccg cgctcgccac ctcgaacgcg acgcgatcgg caactccggc cctcgcggcg
     1921 gcctcgcgcg cggcctcgat cgagggagca tggttgtcgt agccgacgaa ccgcgagttc
     1981 ggataggcct gagccatgat gatagtcgac gcgccgagtc cacagcccac gtcggcaacc
     2041 gtcccgccct gcctcagctt cgcgtcaatc ccgtccagtg ccggtagcca gctgtcgacg
     2101 aggttggcga ggtagccggg cttgaagaag cgctccgtcc cctcgaacag attctcgtcg
     2161 tgttctcccc acagcatccc ggacccatcg cgaaaggcct ggagaatgcg cggaaaggcg
     2221 agcacgagcg agctgacgac ctggtagccc ccgaccacgt aggccgggct gtcttcctgg
     2281 gtcagggcga tggcgtgttc gggcgggagt gagtaccgcc cgctcagcgg gtcgtattcg
     2341 aggtagccac cggccgcctg attcacgagc cactcacgca cgtagcgctc ggctgtctga
     2401 gtgtcttcgc tgagctcggc cgaagtgagg gggcgcgctg ccagtgcgcg gtagaggccg
     2461 agctgatcgc cgatgaccac gagcgctgtg gagagcgtgg tcccgaagtc gccaaccgct
     2521 ttgccgatga actcgttgag cttctgctcg tcgagcatga gtgcctgtgt caccgtcatt
     2581 ctccttcgct tgtcctgcac cgccgaatgc ggccggaggc agcaggggcg aagggccagc
     2641 ccggccgtca ggcgacggtc gtctggatca actgtcgaag gtcctcgtcc cgtacggtct
     2701 tcccggggtg cgcctcgctc agatgcatct gagcgtgctt gatcacctcc tgcggcgttt
     2761 ccgtggtgaa ggcgccgggg cactctacgc ccgcgtcgcg gcatcgaatc gaataggcca
     2821 tttgcgttct cctccctgat cgctggcggg agcctcgcgg ccccgcgatt actctgcgac
     2881 cggcactgcc atcgcgagca cctgctctcg gctcaccgtc atctgatgga cgtcgaatgc
     2941 gtggctggta agcacaacca caagctcgtc ttcctcgccc tcagcggcga acccgcaata
     3001 gcaccggtac accaatttcc ttgccatctg ctgacctcct gaccgcgccc ctactatctc
     3061 gacacgcctt tctgcgtctt tggcagaatg ttggcgcaag cttggaactt cggaatgcga
     3121 ctctacctgt ccggacgcac ggcgatcgag ggcgagggcg ccctccttga ccagcgtgcg
     3181 ctcggtgggc gtcagggccg ccttg
//
LOCUS       DAORPG010000161         3185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2911_length_3185_cov_3.656550, whole genome shotgun sequence.
ACCESSION   DAORPG010000161 DAORPG010000000
VERSION     DAORPG010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2911_length_3185_cov_3.656550"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PLX85_04660"
     CDS             complement(<1..501)
                     /locus_tag="PLX85_04660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRC62494.1"
                     /translation="MNRSFDAPGTTPAHDDFALANRATPLTPVIAAEIAERGPMTFAR
                     FMSLALGHPEHGYYSRPDLAWGAAGDFETSPEVHPVFGYLWARQILECWERLGRPTEF
                     ALVEPGAGSGAFAVATLASAVWSGPALADTKLTIATVNNGDMIRMQKLTDDFTAKNPG
                     ISLEWVT"
     gene            complement(521..1171)
                     /locus_tag="PLX85_04665"
     CDS             complement(521..1171)
                     /locus_tag="PLX85_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62495.1"
                     /translation="MGVTRILHAAGNYRETLPLARLPEVDAIEADVWLRSGDVFVNHA
                     RPLGPIPYTIGSDGLRRNDEDRVNVAELLAAVDGCARLVLDLRSWFGDPAPDIMRALH
                     PLRDRSHLLVSCESWAIADRLRVWLPDLLVAYSVRSEGQLRRFVQGRIGGGLAETAVM
                     VRHTLLRSAAEVESLRRWASTIGVWTVDDEQRARQLAVWGVDSITSNELSVLAALG"
     gene            complement(1187..1720)
                     /locus_tag="PLX85_04670"
     CDS             complement(1187..1720)
                     /locus_tag="PLX85_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRC62496.1"
                     /translation="MRDPLDAGAARARLRPVQPATAPDLSFVVTSETLEYATEHALQF
                     IDITADVRQVIARSGLATGQVSVVSAHTTAAVIVNEHEPLLLNDMARVLSRLAPAGDY
                     YEHNDFSIRTVNMTAEECANGHAHCQHLFLGASETIIVENFEMKLGPWQSIFLVELDH
                     ARRRSVTVQAMGLGRQG"
     gene            complement(1813..2655)
                     /locus_tag="PLX85_04675"
     CDS             complement(1813..2655)
                     /locus_tag="PLX85_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC62497.1"
                     /translation="MTAFDDTLGRFRAAVAGPMSLVPLARPALLIAQAEYPGLDVEAY
                     EERLHDLGAHLEARIHPSADVRTQLAEAHRLLFEEYGFHGNEDEYGDPKNLYLNDVME
                     RRTGIPVTLAILYVEVCRTAGLDAHAVGLPGHVIVRVDSPEASVLVDPFNAGSELTEE
                     QCRQLVRGVFGRRTPFREHFLEPVTSRQVTQRLLHNLKAGYLQRGDEERAGRAIEFLL
                     ALFPWDLDEVRDRGMLRERVGDLPAALADLEEYVRYRAGARDIQTVNEAVRSLRRQTG
                     AAER"
     gene            complement(2652..>3185)
                     /locus_tag="PLX85_04680"
     CDS             complement(2652..>3185)
                     /locus_tag="PLX85_04680"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HRC62498.1"
                     /translation="GSIPTSYIVVYRLTGRDIRTMGTGNPGTMNVVDTVGLRAGLVVG
                     LGDICKGMAAVGVAYLAGLSDRDAVLAGMCAVAGHDWSIFLRLDGGNGMAAAVGALIA
                     LLPEAALIAVPAAIGLYVLGISRRMAGIVGLLLLLALAYTFNAPEVRLIGAVVLLTFT
                     VIKIFRFEGFSPARPGR"
BASE COUNT          495 a         1123 c         1096 g          471 t
ORIGIN      
        1 cgtcacccat tccaggctga tgccgggatt tttggcggtg aaatcatccg tcagcttctg
       61 catccggatc atgtcgccgt tgttgacggt ggcgatggtc agcttggtgt cggcgagggc
      121 cgggccgctc cagacggcgg aagcgagagt cgcgacggca aacgcgcccg aaccggcacc
      181 cggctccacc agcgcgaact ccgtcggccg ccccagccgc tcccagcact cgaggatctg
      241 tcgcgcccag aggtacccga acaccgggtg cacctcgggc gacgtctcga agtcgccggc
      301 cgcgccccac gcgaggtccg gccggctgta gtacccgtgc tcgggatgcc cgagggccag
      361 cgacatgaag cgcgcaaagg tcatcggccc gcgttccgcg atctcggccg cgatgaccgg
      421 agtcagcggc gtcgcccggt tcgcgagcgc gaagtcgtcg tgcgccggtg tcgtgccggg
      481 cgcgtcgaaa gagcggttca tccggcgatc gtagcggccg tcagccgagc gcggcgagca
      541 cgctcagttc gttgctcgtg atgctgtcga cgccccacac cgcgagctgt cgcgcgcgct
      601 gctcgtcgtc caccgtccac accccgatcg tcgaggccca gcgccgcaat gactccacct
      661 ccgcggccga gcgcagcagc gtgtgacgca ccatcaccgc cgtctccgcg agcccgccgc
      721 cgatgcggcc ctgcacgaac cgccgcagct gcccctcgga acggaccgag taggcgacga
      781 gcaggtcggg cagccacacg cggaggcggt cggcgatcgc ccacgactcg cagctcacga
      841 gcaggtgcga gcggtctcgc agcgggtgca gcgcacgcat gatgtccggc gccgggtcgc
      901 cgaaccacga ccgcaggtcc agcacgagcc gcgcgcagcc gtcgaccgcc gcgaggagtt
      961 ccgccacgtt gacgcggtcc tcgtcgttcc gccgcagtcc gtcgctgccg atggtgtagg
     1021 ggatgggacc gaggggccgt gcgtggttga cgaagacgtc acctgagcgg agccagacgt
     1081 ccgcctcgat ggcgtcgacc tctggcagcc gcgcaagcgg gagggtctcg cggtagttgc
     1141 ccgcggcatg gaggatgcga gtgacgccca tggtgcggcc gaggagctag ccctgccggc
     1201 cgaggcccat cgcctggacc gtgaccgagc gccggcgcgc gtggtccagt tccaccagga
     1261 agatgctctg ccaggggccc agcttcatct cgaagttctc gacgatgatc gtctcgctcg
     1321 cgcccaggaa gaggtgctgg cagtgcgcgt ggccattcgc gcactcctcg gcggtcatgt
     1381 tgaccgtccg gatcgagaag tcgttgtgtt cgtagtagtc gcccgccgga gcgagccgcg
     1441 acagcacgcg agccatgtcg ttgagcagca gcggctcgtg ctcgttcacg atcaccgcgg
     1501 ccgtcgtatg cgccgacacg acgctcacct ggcccgtcgc gagtcccgag cgcgcgatca
     1561 cctgccgcac gtcggcggtg atgtcgatga actgcagcgc gtgctccgtc gcgtactcga
     1621 gcgtctccga ggtcacgaca aacgacaggt cgggtgcggt cgccggctgc acggggcgca
     1681 gccgcgcccg ggcggcgccg gcgtcgagcg ggtctctcat cggtatctgg ttgccttctg
     1741 gtaagtgaag agcggcctgc tcaaagggta gcagtggccc cgaactggcg ccagcgcagg
     1801 cgggtgaaac ggctaccgtt cggccgcccc ggtctgccgc cgcagcgacc gcactgcctc
     1861 gttcaccgtc tggatgtcgc gcgccccggc ccggtaccgc acgtactcct cgaggtccgc
     1921 gagggcggct ggtaggtcgc cgacccgctc gcgcagcatc ccccggtcgc gcacctcgtc
     1981 gaggtcccag gggaacagcg ccagcaggaa ttcgatggct cggcccgcac gctcctcgtc
     2041 cccccgctgg aggtagcccg ccttcaggtt gtgcagcagc cgctgggtca cctgcctgga
     2101 ggtcaccggc tcgaggaaat gctcccggaa cggcgtgcgc cggccgaaga cgccgcggac
     2161 cagctgccgg cactgctcct cagtcagctc gctgccggca ttgaacggat cgaccaggac
     2221 gctcgcctca ggactgtcta cgcggacgat gacgtggccc ggcagtccca ccgcatgggc
     2281 gtcgagcccg gccgtgcggc agacctccac atagaggatc gcgagcgtga ccgggatgcc
     2341 ggtgcgccgc tccatgacgt cgttcaggta gaggttcttc gggtccccgt actcgtcttc
     2401 gttgccgtgg aacccgtact cctcgaacag tagccggtgt gcctcggcca gctgagtgcg
     2461 cacgtccgcc gaggggtgga tacgcgcctc gaggtgcgcg cccaggtcgt gcaggcgttc
     2521 ctcgtaggcc tccacgtcga ggccgggata ctcggcctgc gcgatcagca gcgcgggccg
     2581 cgccagtggc acaaggctca ttggccccgc caccgcggca cgaaaacgcc cgagcgtgtc
     2641 gtcgaacgcc gtcatcggcc cggccgggcg ggggagaagc cttcgaagcg gaagatcttg
     2701 atgacggtga acgtgagcag gacgacggcc ccgatcagac gcacctcggg cgcgttgaag
     2761 gtgtaggcga gcgcgagcag cagcagcagg ccgacgatgc ccgccatccg cctcgatatg
     2821 ccgaggacat acagcccgat ggcggcggga acggcgatca gcgccgcctc gggtagtagc
     2881 gcgatcagtg cgcccacggc cgctgccatc ccgttgccgc catcgagacg caagaagatc
     2941 gaccagtcgt gtccggcgac cgcgcacatg ccggccagta ctgcgtcgcg gtcgctgagc
     3001 cccgcgaggt acgccacgcc aaccgcggcc atgcccttgc agatgtcgcc aaggcccacc
     3061 accagtccgg ctcgcaggcc gaccgtgtcc acgacattca tcgttccggg gttgccggtg
     3121 cccatcgtcc ggatgtcacg cccggtgagc cggtacacga cgatgtacga ggtcggaatc
     3181 gagcc
//
LOCUS       DAORPG010000162         3184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2915_length_3184_cov_3.529562, whole genome shotgun sequence.
ACCESSION   DAORPG010000162 DAORPG010000000
VERSION     DAORPG010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2915_length_3184_cov_3.529562"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLX85_04685"
     CDS             complement(<1..295)
                     /locus_tag="PLX85_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HRC62499.1"
                     /translation="MASSTRGSRVQYHCSFCGKNQDQVKRLIAGPGAVYICDECVELC
                     QEIINEETQAAGKTPAKGQRPRLRTPQEIVNQLDEYIVGQERTKRVLSVAVYNH"
     gene            complement(365..1006)
                     /locus_tag="PLX85_04690"
     CDS             complement(365..1006)
                     /locus_tag="PLX85_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRC62500.1"
                     /translation="MTPSDWTQPQNIVPMVIETTARGERAYDIYSLLLKERIIFLGTP
                     IDDQIANIIVAQLLFLDREDPDRDIHMYIHSPGGVITAGLAIYDTMNLIRPDVSTIAV
                     GATASMGTVLLCSGTKGKRYALPNATVHMHQALGGARGQASDIEIAAREILRNNEIIR
                     GILSRNTGQPMERIQRDTDRDFYMSADQAKEYGLVDEILSRPDENRNGTGSLS"
     gene            complement(1054..2580)
                     /gene="tig"
                     /locus_tag="PLX85_04695"
     CDS             complement(1054..2580)
                     /gene="tig"
                     /locus_tag="PLX85_04695"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRC62501.1"
                     /translation="MGVTTERLPRSLVALEIEVDDERLEAQMDKAVRRLSQRVRIPGF
                     RPGKAPRQVVERTLGRPALLQEALEELLPDVYNEAIESQDIQAIGQPEFDLKSTEPLV
                     VSATVPVRPTIDLRDYRALRAPRPEAQAPDGQVEEALTSLRRRYATLEPVDRAVDWDD
                     VVRADVTVSLEGQTEPPHEEEGAEFAVTRDGVVSLPGFLDHLIGLERGGPYEIEFSLP
                     EDYPAAELAGKQAHYTVTILEVKQQILPELDDDFVKSLDEDGIETVEQLRERVTQNVE
                     AGAKAEADNAYREEVLDLLVASSELDYPEILVEREIDRMVDRESNHAAHTREDLDRWL
                     QAIGRSEDEVRDSLRAQADLAVRRALVLGELASREELAVTEEEIEGEIMRMASQFDIR
                     TAEGLNEEQRDAIRAIFDSPDNRASISNELMTTKTLQRLEEIASAAEEEGETRTRGSR
                     RRRGARAEGDTADEGEDGAEDTLPEGAGATADESEGATGTLEGDTGASSPEAPPSEAE
                     "
     gene            2736..>3184
                     /locus_tag="PLX85_04700"
     CDS             2736..>3184
                     /locus_tag="PLX85_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRC62502.1"
                     /translation="MRAFTFRAPARLAGILSVIVLTATVVACGDDDGFHSATAGELTV
                     ITSLPAPGFWTGTPDRGFEGGMEYRIARALADEFGLQLKVMDVPFDRIAAADFGDADL
                     AISQVLVTPEREQRLTFSTSYFAITSGALVRRGTTVRDLQEARTRQWV"
BASE COUNT          448 a         1064 c         1076 g          596 t
ORIGIN      
        1 agtggttgta gaccgcgacc gagaggacgc gcttcgtgcg ttcctggccg acgatgtact
       61 cgtcgagctg gttgacgatc tcctggggag tgcgcaggcg gggccgctgc cccttggccg
      121 gggtcttgcc cgccgcctgc gtctcctcgt tgatgatctc ctggcacagc tcgacgcact
      181 cgtcgcagat gtagacggcg cccgggccgg cgatcaggcg cttgacctga tcctggttct
      241 ttccacagaa ggagcagtgg tactgcactc ggctgccgcg cgtgctactc gccatcggac
      301 cgggccccct ctgccctcgt cgtcacgccg ccgccccgaa cgtccttatt ctgcctcagg
      361 cagcctagct gagcgagcca gtgccattcc ggttttcgtc gggccggctg aggatctcgt
      421 ccacgaggcc gtactccttc gcctggtccg cgctcatgta gaagtcgcgg tcggtgtcgc
      481 gctggatgcg ctccatcggc tggccggtgt tgcgcgagag gatgccgcga atgatctcgt
      541 tgttgcgcag gatctcgcga gccgcgatct caatgtcgga ggcctgaccc cgggcgccgc
      601 cgagcgcctg gtgcatatgc accgtcgcgt tcggcagagc gtagcgcttg cccttcgtgc
      661 cggagcagag gagcacggtg cccatcgagg cggtcgcgcc gacggcgatc gtcgagacat
      721 cggggcggat gaggttcatc gtgtcgtaga tcgccaggcc agcggtgatc acgccgcccg
      781 gcgagtggat gtacatgtgg atgtcgcggt ccgggtcctc gcggtcgagg aacagcagct
      841 gcgcgacgat gatgttcgcg atctggtcgt cgatcggcgt gccaaggaag atgatgcgct
      901 ccttgagcag gagcgagtag atgtcgtagg cgcgctcgcc gcgtgcggtg gtctcgatga
      961 ccatcgggac gatgttctga ggctgggtcc agtcgctcgg tgtcatgtgc tcgtatcctg
     1021 cgctttcttg ctgcgttccg gtcgcgagtt cgactactcg gcctcggagg gaggagcttc
     1081 cggcgaagac gcgccggtgt ctccctctag cgtaccggtt gcgccctcgc tctcgtccgc
     1141 ggtggcacct gcaccttcgg gcagggtgtc ctccgcaccg tcttcacctt cgtccgcggt
     1201 gtccccttcg gcgcgggccc cgcgccgacg gcggctgccg cgggtgcgcg tctcgccctc
     1261 ttcctctgcc gccgaggcaa tctcctcgag gcgctggagg gtcttcgtcg tcatcagctc
     1321 attgctgatc gaggcgcggt tgtcggggct gtcgaagatc gcgcggatcg cgtcgcgctg
     1381 ctcctcgttg aggccctcgg ctgtgcggat gtcgaactgc gacgccatcc gcatgatctc
     1441 gccctcgatc tcctcttcag tcaccgcgag ttcctcgcgg ctggcgagct cgccgagcac
     1501 gagggcgcgg cggaccgcaa ggtcggcctg ggcccgcagg ctgtcgcgca cttcgtcctc
     1561 gctccggccg atggcctgga gccagcggtc caggtcctcg cgagtgtggg ccgcatgatt
     1621 cgactcacgg tcgaccatgc ggtcgatctc gcgttccacg aggatctcgg ggtagtcaag
     1681 ctcggaggac gcgaccagca ggtcgagcac ttcctcccgg taggcgttgt cagcctcggc
     1741 cttcgcccca gcctcgacgt tctgtgtcac acgttcgcgc agctgctcca cggtctcaat
     1801 gccgtcctcg tcgagcgact tcacgaagtc gtcgtcgagt tcgggaagta tctgctgctt
     1861 cacctcgaga atcgtgacgg tgtaatgggc ctgcttgccg gccaactcgg ccgcggggta
     1921 gtcctcaggg aggctgaact cgatctcgta gggcccgccg cgctcgaggc cgatcaggtg
     1981 gtcaaggaag ccgggcagcg acacaacgcc gtcccgggtc accgcgaact ccgcgccctc
     2041 ttcctcgtgc gggggttcag tctgcccctc gagagaaacg gtcacatcgg cgcgcacgac
     2101 atcgtcccag tcgaccgcgc ggtcgacggg ctccagcgtg gcgtagcggc gtcgcagcga
     2161 ggtcagcgcc tcctcgacct gaccatcggg cgcctgcgcc tcagggcgcg gcgcgcgcag
     2221 cgcacggtag tcgcgcaggt cgatcgtcgg ccgcacaggc acggtcgccg agaccacgag
     2281 aggctcggtc gacttgaggt caaactcggg ctgcccgatg gcctggatat cctggctctc
     2341 gatggcctcg ttgtagacat cgggcagcag ctcctcgagg gcctcctgaa gcagcgcagg
     2401 gcgtcccagg gtgcgctcga ccacctggcg gggcgccttg cccggacgga agccggggat
     2461 ccgcacgcgc tgcgagaggc ggcgaaccgc cttgtccatc tgcgcctcga gccgttcgtc
     2521 gtccacctcg atctcgaggg cgacgagcga acggggcaaa cgctctgtag tcacacccac
     2581 ctggtgcgcc cctgtcccct accgcttaca tgttgtgtgt tcgaaatgag tatagcaccc
     2641 caccatttac cggcccgagt gaccctgaga agcggcggat gaggttgaac tcacgtacgc
     2701 tctcgcatgc tcctcacgag gtgaaaggaa cccgcatgcg ggcgttcacg ttccgcgctc
     2761 ccgcgcgcct cgccggcatc ctgagcgtga tcgtgctgac cgccaccgtc gtggcctgcg
     2821 gcgacgatga cgggttccac tcggcgaccg ccggcgagct gaccgtgatt accagtttgc
     2881 ccgcgccggg cttctggacc gggacgccag accgcggctt cgagggcggg atggagtacc
     2941 gcatcgccag ggcgctggcc gacgagttcg ggctgcagct gaaggtgatg gatgtgccgt
     3001 tcgaccgtat cgcggcggcg gacttcgggg acgccgacct ggccatctcg caggtcctgg
     3061 tgacgcccga acgggaacag cgactcacgt tctcaacctc gtacttcgcg atcacgagcg
     3121 gagcgctcgt ccgccgcggc acgacggtac gtgacctgca agaggcgcgg actcggcagt
     3181 gggt
//
LOCUS       DAORPG010000163         3181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2921_length_3181_cov_3.247601, whole genome shotgun sequence.
ACCESSION   DAORPG010000163 DAORPG010000000
VERSION     DAORPG010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2921_length_3181_cov_3.247601"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..>3181)
                     /gene="dnaE"
                     /locus_tag="PLX85_04705"
     CDS             complement(106..>3181)
                     /gene="dnaE"
                     /locus_tag="PLX85_04705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRC62503.1"
                     /translation="DGEVRSHLTLLAETKQGYSNLCRLSSIAFGLLEEDTHAKEERRL
                     DACLAVEHLEAHAEGLIVLTGCREGRLARLVEEGRGEEAARWLGRLIAWFGRDDVFVE
                     LQDNLVFGDRPRNHALVALAERAGVSVVGTGNVHYHERDRHRLQDVLTAIRHRKTLDE
                     SHRERRPNAEFYLRPPEEQAWRFEQYHPEAASNSVRIAWRCRFDLTEDLGYRLPQPDL
                     GEGRQPIDELRRICQERLDGQYSGRRQRYRVAAQERLDHELALIERHQLSGFFLIYHQ
                     IFELAKDVAREVRGANSARQFAKLPPGRGRGSSVASIVCYLIGLSHIDPIANRLTVGR
                     FLNEDMRTFPDIDLDFARDIRRVLIERVHDTWGKDHAALVAIFPTYRLRSALRDVGKA
                     LGLPLSELDRLAKRASPHDGISQLPDELARHPEFADRLEAQGWRDLAELSQQLAGFPR
                     HLSQHVGGMVISSEPLIDYVPCQPAAWPNRYLCHWDKDSVDDARMVKIDFLGLGMLST
                     VEETVELIAENGPQIDLSTIAFDDEAVFDDIQRGDTVGVFQIESRAQIAMLPRTQPRN
                     LDDLTVQVSIVRPGPIVGGAVNPYVREREQQRRLGEAYRPPKVHSCVDDVLEETLGVV
                     LFQEQVIDVARGMGGFTAGQAEQFRRAMSRKRSLLAMQQYEEAFMAGARAQGVPQAAA
                     ERMFKNLLGFAEFGFPKSHGAAFGLLAYQTTWLKHYYRAPFFCALFNNQPMGFYPPHV
                     LTNDAKRHGVEVVRPDINCSMARCSVEHHTGRHGGYAHGAVRVGLGYVRGVGEAGATR
                     IEDERRREGPYQSLFNLVQRTGLSREAVESLIRVGACDDFGLNRRELLWQLGLFWGGM
                     QQAALSVPRERQLRLPLSVEQDQVTLADFGAYQRMAADYELLSLSPDSHPMQFLRRTL
                     GEGVASSQHLRSMSGGTEVDVAGLVVCRQRPMTAKGIIFLLLEDEFGMVNVLVSRELS
                     EQFKDQIRTEPFILVRGVLEERAGEQRTLVASHLRELVPAEMFRTPMGKSWG"
BASE COUNT          472 a         1153 c         1051 g          505 t
ORIGIN      
        1 ctcgaggccg ccctgcgcga gtgccgcgtc tggcgggacg ccggcatcga ggtcgaggac
       61 acgtcggaag ggcctcgctg gtcggcgcgg cggtagggcg tcgcctcagc cccacgactt
      121 gcccattggc gtgcggaaca tctcagcggg cacgagttcg cgcaggtgtg aggcgaccag
      181 cgtgcgttgc tcgcccgcgc gctcctcgag tacgccgcgc acgaggatga acggctccgt
      241 gcggatttga tctttgaatt gctccgacag ttcgcggctc acgagcacgt tcaccatccc
      301 gaactcgtcc tcgaggagca ggaagatgat ccccttcgcg gtcatcggcc gctgacggca
      361 taccacgagg cccgcgacgt ccacctcggt gccgcctgac atcgagcgca ggtgctggct
      421 cgaggccaca ccctcgccca gcgtccggcg caggaactgc atcgggtggc tatccggcga
      481 cagtgacagc agctcgtagt cggccgccat ccgctggtac gccccgaagt cggccagcgt
      541 cacctggtcc tgctccaccg atagcggcag ccgcagctgc cgctcgcgag gcacggaaag
      601 cgccgcctgc tgcatgccgc cccagaacag cccgagctgc cacagcagct cccgccggtt
      661 gagcccgaag tcgtcgcacg ccccgacgcg gatcaggctc tcgaccgcct cgcggctcag
      721 tcccgtccgc tgcacgaggt tgaacagcga ctggtacggc ccctctcggc ggcgctcgtc
      781 ctcgatgcga gtcgcccccg cttcgcccac cccgcgcacg tacccgaggc cgacgcgcac
      841 ggcgccgtgc gcatagccgc cgtgcctgcc ggtgtgatgc tcgaccgagc agcgcgccat
      901 gctgcagttg atgtccggcc gcacgacctc gacgccgtgg cgtttcgcgt cgttggtgag
      961 cacgtgcggc gggtagaagc ccatcggctg gttgttgaac agcgcgcaga agaacggcgc
     1021 acggtagtaa tgcttcagcc atgtcgtctg gtacgcgagc agcccgaagg cggccccgtg
     1081 gctcttcgga aacccgaact ccgcgaagcc gagcagattc ttgaacatgc gctcggcggc
     1141 cgcctgcggc accccctgtg cccgcgcgcc cgccatgaac gcctcttcgt actgctgcat
     1201 cgcgaggagt gagcgcttcc ggctcatcgc gcgccggaac tgctccgcct gccccgccgt
     1261 gaagccgccc atcccccgcg cgacgtcgat cacctgctcc tggaacagca ccacccccag
     1321 cgtctcctcg aggacgtcgt cgacgcagga gtgcaccttc ggcggccgat acgcctcgcc
     1381 gagacggcgc tgctgctcgc gctcgcgcac gtacgggttc actgccccgc ccacgatcgg
     1441 ccctggccgc acgatcgaca cctgcaccgt caggtcatcg aggttcctcg gctgcgtccg
     1501 aggcagcatc gcgatctgcg cccgtgactc gatctggaag acgccgacgg tatcgccgcg
     1561 ctggatgtcg tcgaagaccg cttcgtcgtc gaaggcgatc gtcgagaggt cgatctgcgg
     1621 cccgttctcc gcgatcaact ctacggtctc ctcgacggtc gacagcatgc cgaggccgag
     1681 gaagtcgatc ttcaccatgc gtgcgtcgtc gacgctgtcc ttgtcccagt gacacaggta
     1741 gcggtttggc catgccgccg gctggcacgg gacataatcg atgagtggct ccgaggagat
     1801 gaccatcccg ccgacgtgct gcgagaggtg gcgcgggaac cccgccagct gctgcgacag
     1861 ctccgcgagg tcacgccagc cctgcgcctc gaggcggtcc gcgaactcgg ggtgccgcgc
     1921 cagctcgtcc ggcagctgcg agatgccgtc gtgcgggctg gcgcgctttg ccagccggtc
     1981 gagctctgac agcgggaggc cgagggcctt gcccacgtcg cgaagcgcgc tgcgcagccg
     2041 gtaggtcgga aagatcgcca cgagggcggc gtggtccttg ccccaggtgt cgtgcacccg
     2101 ttcgatcagc acccgccgga tgtcgcgcgc gaaatcgagg tcgatgtcgg ggaacgtccg
     2161 catgtcctcg ttcaggaagc gtcccaccgt gagccggttc gcgatcgggt cgatgtgcga
     2221 gagcccgatc aggtagcaca cgatcgaggc aaccgaggag ccacgtcccc ggccgggcgg
     2281 cagcttcgcg aactggcgcg cgctgttggc tccgcgcacc tcgcgcgcca cgtcctttgc
     2341 cagctcgaag atctggtggt agatgaggaa gaacccgctg agctggtggc gctcgatcag
     2401 cgccagctcg tggtcgagtc gctcctgggc cgccacgcgg tagcgctgcc gccgcccgct
     2461 gtactgccca tcgaggcgct cctggcagat tcgccgcagc tcgtcgatcg gctgacggcc
     2521 ctcgcccagg tccggctgcg gcagccggta cccaaggtcc tcggtaaggt cgaagcggca
     2581 gcgccaggcg atgcgcaccg agttcgacgc cgcctccggg tggtactgct cgaagcgcca
     2641 cgcctgctcc tcgggcggcc gcaggtagaa ctccgcgttc ggccgccgct cgcggtgcga
     2701 ctcgtcgagg gtcttgcggt gccgaatcgc ggtgagcacg tcttgcagcc ggtgccggtc
     2761 acgctcgtgg taatgcacgt tgcccgtacc cacgacgctc acacctgcgc gctccgccaa
     2821 tgccaccagc gcgtggttgc gcggccggtc gccgaacacg aggttgtcct gcagctcgac
     2881 gaagacgtcg tcgcgcccga accaggcgat gagccgtccg agccagcggg cggcctcctc
     2941 gccgcgtccc tcctccacca gccgggcgag ccgcccctcg cggcacccgg tgagcacgat
     3001 cagcccctcg gcgtgcgcct caaggtgttc gaccgcgagg caggcatcga ggcgtcgctc
     3061 ctccttcgca tgcgtgtcct cctcgagcag tccgaacgcg atgctcgaga gcctgcagag
     3121 gtttgagtac ccctgcttcg tctccgccag cagcgtgagg tgcgagcgca cctcgccgtc
     3181 g
//
LOCUS       DAORPG010000164         3180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2922_length_3180_cov_3.424000, whole genome shotgun sequence.
ACCESSION   DAORPG010000164 DAORPG010000000
VERSION     DAORPG010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2922_length_3180_cov_3.424000"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PLX85_04710"
     CDS             complement(<1..277)
                     /locus_tag="PLX85_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HRC62504.1"
                     /translation="MAFVITQPCIDTQDQACVEVCPVDCIHFEEGADRMLYINPLECI
                     DCGACQPACPVQAIFPEGDVPGDQKPFIEINAQWFQDAAAARAKVAPM"
     gene            complement(376..1461)
                     /locus_tag="PLX85_04715"
     CDS             complement(376..1461)
                     /locus_tag="PLX85_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HRC62505.1"
                     /translation="MADDIERLLAELSEAPGPSGFEGGIRSVVRREVAALVDDLETDG
                     IGSLIARLGGAEARPRVMLSAHMDELGLMVRRITSDGYLMFQPLGGWLDQALVNQRWR
                     VLTSRGEVLGVTGIKTVHVMKPDERERVFKRDGLFIDVGARDRADAEERLGIRPGDPV
                     APLSPFATFAGGELLLGKAWDDRAGVGVLIEVLRRLQAEPGPNTVYGAVTVQEEVGLR
                     GAGTSANRVAPDIGINLESGVAGDYPGISEDEAQERLGRGPAIFLHDSSMLPNLKLRD
                     LVVEVAGDLGVDLQFNVLTGYGQDGAAMQQAYGGAPVINITVPTRYLHSHNSVIHRGD
                     FLGAVTLVTELVRRLDAVAVEELRRFD"
     gene            1531..2295
                     /locus_tag="PLX85_04720"
     CDS             1531..2295
                     /locus_tag="PLX85_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HRC62506.1"
                     /translation="MRYRVRMTPSAVRTRPWELEGGLLRSPLLSSMGLVAGFTTRAFG
                     SMGDTTTPREVQQRNRSDVATLLGFGGVARAKQVHGDEVVQVTDVPAAWPEADAMWAA
                     RRGLLLGVVAADCVPVLVASEDGDVLGVAHAGWRGTSLAIASRLVEAMTVSGGGTVVA
                     ALGPSIGPCCYTIDDERARLIAERLGPGNEDLVAGGRFDLWAANARQLLAAGVTSVEV
                     SGICTRCGSADVWSYRGRDSHPEYGTCMAFLGRPEG"
     gene            complement(2300..>3180)
                     /locus_tag="PLX85_04725"
     CDS             complement(2300..>3180)
                     /locus_tag="PLX85_04725"
                     /inference="COORDINATES: protein motif:HMM:NF019640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC62507.1"
                     /translation="MPEAEAREVFGHGGIIAGTARPIGRAVAVQRPEAGYIVSGRWPF
                     ASGSSHADWFMGECVVYDGDQVRLDAQGNQVTRAVFVPRSEVTTYDTWHTTGLRGTAS
                     NDFSVTEVFVPEGRGFQMLVVEPQHPWVLYAYPQLVFMNHGSHALGLARAALDAATEA
                     VRTKRGWGDQPLREVLRVQSVVAEATALIESARCYLYATAEELWAAAQSDAAETQVLR
                     SRVRLAASHAARSSVQAIDLLHSTLGTTAIFVSNPLERIFRDIHTAAAHVMIGQLTYQ
                     AAGRVELGMEPDFPFF"
BASE COUNT          475 a         1126 c         1084 g          495 t
ORIGIN      
        1 ccatgggcgc aaccttcgcg cgcgccgcgg cggcatcctg gaaccactgc gcgttgatct
       61 cgataaacgg cttctggtct cccgggacgt cgccctctgg gaagatggcc tgcaccgggc
      121 aggccggctg gcaggcgccg caatcgatgc attcgagggg gttgatgtac agcatccggt
      181 ccgcaccctc ttcgaagtgg atgcagtcga ccgggcatac ctcgacacag gcctggtctt
      241 gcgtgtcgat gcagggctgc gtgatcacga acgccatggc gctcacctct cgagacagaa
      301 agccgcgctc tatcgcgcaa atcgtacgcg aggcttgcgg cgcagtccat gcgcgcgcat
      361 agcctccagg cgggctcagt cgaagcgccg cagctcctcg acagcgaccg catcgagccg
      421 gcgcaccagc tccgtgacca gggtgaccgc cccgaggaag tcgccccgat gaatgacgct
      481 attgtggctg tggaggtacc gcgtcgggac cgtgatgttg atcacgggcg ccccgccata
      541 cgcctgctgc attgcggcgc catcctgacc gtagcccgtg agcacattga actgcaggtc
      601 gacgcccagg tcgccggcaa cctcgaccac gaggtcgcgc agtttcaggt tcggaagcat
      661 cgagctgtca tgcaggaaga tcgccgggcc gcggcccagg cgctcctgcg cctcgtcctc
      721 tgagatgccg ggatagtcgc cagcgacgcc cgactcgagg ttgatcccga tatccggggc
      781 cacgcggttc gcgctcgtgc ccgcgcctcg cagcccgacc tcctcctgca ccgtcaccgc
      841 gccatagacg gtgttcgggc ccggctccgc ctggagtcgg cgaagcacct cgatgagcac
      901 gccgacgccg gcgcggtcgt cccacgcctt ccccagcagc agctcgccgc cggcgaaggt
      961 ggcgaagggg ctgagtggcg cgacggggtc cccgggacgg ataccgaggc gctcctcggc
     1021 gtccgcgcgg tcgcgtgcgc cgacgtcgat gaacaggcca tcgcgtttaa acacccgttc
     1081 gcgctcgtcg ggcttcatga cgtgcaccgt cttaatccca gtcacgccca acacctcgcc
     1141 acgactcgtc aggacgcgcc agcgctggtt gaccagcgcc tggtcgagcc agccgcccag
     1201 gggctggaac atgaggtacc cgtccgaggt gatccggcgc accatcaatc cgagctcgtc
     1261 catgtgcgcg ctgagcatca cgcgcggccg ggcctcggcg cccccgagcc gcgcgatcag
     1321 cgacccgatc ccatctgttt cgaggtcgtc gaccagcgct gcgacctcgc ggcggaccac
     1381 gctgcggatg ccgccctcga acccggaggg gcctggcgct tccgacagct ccgccaacag
     1441 ccgctcgatg tcgtccgcca tgtcgcctcc ggttcgttga tccgtctcgc tcagcggcga
     1501 ttctaatcgg cggctccgag gagcgcggcc atgcggtacc gtgtccgcat gacgccgagc
     1561 gccgttcgga cgcggccgtg ggagctcgag ggtgggctgc tgcgctcacc gctgctgtcc
     1621 tcgatgggac tcgtcgcggg tttcaccacc cgggccttcg gctcgatggg ggacaccacc
     1681 acgcctcgtg aggtgcagca gcgcaaccgc agcgacgtgg ccacgttgct cggattcggg
     1741 ggcgtggcgc gagcgaagca ggtgcacggc gatgaagtcg tgcaggtcac cgacgtgccc
     1801 gcggcgtggc ccgaggccga tgcgatgtgg gcggcgcgcc gggggttgct gctgggagtc
     1861 gtggcggccg attgcgtgcc ggtgctcgtc gcgtccgagg acggcgacgt cctgggcgtg
     1921 gcccatgccg gctggcgcgg gacttcgctc gcgatcgcgt cgaggctggt cgaggcgatg
     1981 accgtgtctg gcggcggcac cgttgttgcg gcgctcggcc cgtcgatcgg gccctgctgc
     2041 tacacgatcg acgacgagcg agcgcggctg atcgccgagc gcctcgggcc cgggaacgag
     2101 gacctcgtcg ccggcggccg tttcgacctc tgggccgcaa acgcccgaca gctgctggca
     2161 gccggggtga cctccgtcga ggtcagcggc atctgcacgc gctgtgggag cgcggacgtg
     2221 tggtcgtacc gcggccggga ttcgcacccc gaatacggca cgtgcatggc gttcctcggc
     2281 cgcccagagg ggtgaccggc tagaagaacg ggaagtccgg ctccataccc agctcgactc
     2341 gccccgccgc ctggtacgtc agttgcccga tcatcacgtg cgccgcggcc gtgtggatgt
     2401 cccggaagat ccgctcgagg gggtttgaga cgaagatcgc cgtcgtcccc agcgtcgaat
     2461 gcagcaggtc gatcgcctgc acgctcgagc gggccgcgtg gcttgcggcc aggcgcaccc
     2521 tcgatcgcag cacctgcgtc tccgctgcgt ccgactgcgc cgcggcccag agctcctcgg
     2581 cggtggcgta gagatagcag cgagccgact cgatcagcgc cgtggcctcg gccacgaccg
     2641 actgcacgcg tagcacctcg cgcagtggct ggtcgcccca gccgcgcttg gtccgcaccg
     2701 cctcggtagc cgcgtcaagg gcggcgcgcg ccaggccaag ggcgtgactg ccgtggttca
     2761 tgaatacgag ctgcggatac gcgtacaaca cccacggatg ctgcggctcg accaccagca
     2821 tctggaatcc acggccctcc gggacgaaca cctccgtcac cgagaagtcg ttgctcgcgg
     2881 tcccccgcag gccggtggtg tgccaggtgt cgtacgtggt cacctcggag cgaggcacga
     2941 acaccgctcg tgtcacctgg ttgccctgcg catcgaggcg gacctggtcg ccatcgtaga
     3001 caacgcactc gcccataaac cagtcggcgt gactggagcc gctggcgaac ggccagcgcc
     3061 ccgacacgat gtatccggcc tccggccgct gtacggcgac cgcgcggccg ataggccgcg
     3121 ccgtcccggc gatgatgccg ccgtgaccaa acacctcgcg tgcctctgcc tccggcatga
//
LOCUS       DAORPG010000165         3176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2932_length_3176_cov_3.435117, whole genome shotgun sequence.
ACCESSION   DAORPG010000165 DAORPG010000000
VERSION     DAORPG010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2932_length_3176_cov_3.435117"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1746)
                     /gene="leuS"
                     /locus_tag="PLX85_04730"
     CDS             complement(<1..1746)
                     /gene="leuS"
                     /locus_tag="PLX85_04730"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRC62508.1"
                     /translation="MTTQERTEAERYQPQDVERRWQERWAESGLYRIPDHVPGRENWY
                     ALTMFPYTSGDLHIGHWFAMAPSDALARYRRMRGHNVLFPIGFDAFGLPAENAAIKGG
                     SKHPMDWTMANIDRMRGQLKRMGAMFDWEREVNTSAPEYYRWTQWWFLQLYKMGLAYR
                     AEAAANWCPFDQTVLANEQVVDGKCERCDTPVVQRLMEQWFLRTTHYAEELLDNEGLD
                     WPEHVKLMQRNWIGRSEGAEVSFALEAPTEDGTDEIRVFTTRPDTLCGVTFMVLAPEH
                     PLVRQLTTPDRREAVEAYLDAAARATEIERQSTERPKTGVFTGAYCVNRLTGDRVQIW
                     TADYVLYTYGTGAVMGVPAHDQRDFDLAREYDIPVKVVIAPAGWDGADLEAAYTEPGT
                     MVHSGQFDGLPSEQAKKAITDYLEGQGWGRARVVYRLRDWLVSRQRYWGAPIPMVYCD
                     ECGIVPVPESELPVALPYDVEFKPTGESPLARSDEFVHVACPSCSGPARRETDTMDTF
                     MCSSWYFMRYPDARNEAMPVSSEAATAWLPVDQYTGGAEHATMHLLYARFFYKAARDA
                     GVVPGDEPFTRYFAQG"
     gene            complement(1964..2137)
                     /locus_tag="PLX85_04735"
     CDS             complement(1964..2137)
                     /locus_tag="PLX85_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62509.1"
                     /translation="MWVIIAAFVAYFFLVTWQMRRAVGTSDPAARLREARRLLLLVSA
                     GGPLLAILILVAL"
     gene            complement(2180..>3176)
                     /locus_tag="PLX85_04740"
     CDS             complement(2180..>3176)
                     /locus_tag="PLX85_04740"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRC62510.1"
                     /translation="GVYPGGRFFLADQAGLVSLLRSDGSDAGVLLDLRGEVLRGGNEE
                     GLLSVALDPAFPARPYLYAYYSAANPRRTVLSRFEVANDRADAGSKLVIMEQPQPFSN
                     HNGGAVRFGPDNMLYLGIGDGGSEGDPSGNGQNLGVLLAKIVRIDVRNASAARPYEVP
                     RDNPFAGQAGARPEVWAYGLRNPWRMAFDRTDGRLWVGDVGASSVEEVDVVQRGSNYG
                     WNRMEGDRCYSPRTGCDRSGITMPVATYTHDEGCSITGGVVYRGRELPALVGWFLYAD
                     FCSGRVWAVPAAGGEQRELFRDDSERRIASFAEDEAGEVYLLVHGGAVLRLRAGP"
BASE COUNT          499 a         1225 c          972 g          480 t
ORIGIN      
        1 cccctgcgcg aaatagcgcg taaatggctc gtcgcccggc acgacccccg cgtcgcgagc
       61 ggccttgtag aagaagcgcg cgtacagcag gtgcatcgtc gcgtgctccg cgccgcccgt
      121 gtactggtcc acgggcagcc aggcggtcgc ggcctccgag gacaccggca tcgcctcatt
      181 gcgcgcgtcg gggtagcgca tgaagtacca cgaggaacac atgaacgtgt ccatcgtgtc
      241 cgtctcgcgc cgcgctggtc cgctgcacga agggcatgcc acgtgtacga actcgtctga
      301 gcgcgcgagc ggcgactccc ccgtgggctt gaactccacg tcgtacggca gcgcgaccgg
      361 cagctcgctc tccggcaccg gcacgatgcc gcactcgtcg cagtacacca tcgggatcgg
      421 cgcgccccag taccgctgcc gcgacacgag ccagtcgcgc agccggtaca cgacgcgggc
      481 gcgtccccac ccctggccct cgaggtagtc cgtgatcgcc ttcttcgcct gctcgctcgg
      541 caggccgtcg aactgccccg agtgcaccat cgtccccggc tcggtgtacg cagcctcgag
      601 gtcagcgccg tcccaccccg ccggcgcaat gacgaccttc accgggatgt cgtactcccg
      661 ggcgaggtcg aagtcgcgct ggtcatgcgc aggcacgccc atgacggcgc ctgtcccgta
      721 tgtgtacagg acataatctg ccgtccagat ctgcactcgg tcgcccgtca gccggttcac
      781 gcaataggcg cccgtgaaga ctcccgtttt cggccgctcg gtcgactgcc gctcgatctc
      841 cgtcgcgcgc gcagcggcgt cgagatacgc ctcgaccgcc tcgcggcggt ccggcgtcgt
      901 cagctggcgc acgagcgggt gttccggcgc cagcaccatg aacgtcacac cgcacagcgt
      961 gtcgggccgg gtggtgaaca cccggatctc gtccgtcccg tcctccgtcg gcgcctcgag
     1021 ggcgaacgac acctcggcgc cctccgagcg tccgatccag ttccgctgca tgagcttcac
     1081 gtgctcgggc cagtccagcc cctcgttgtc cagcagctcc tcggcatagt gggtcgtgcg
     1141 gaggaaccac tgctccatca gccgctgcac gacgggcgtg tcgcaccgct cgcacttccc
     1201 gtcgaccacc tgctcgttcg ccagcaccgt ctggtcgaac gggcaccagt tcgccgccgc
     1261 ctccgcgcgg tacgccagcc ccatcttgta cagctgcagg aaccaccact gcgtccagcg
     1321 gtagtactcg ggcgccgagg tgttcacctc gcgctcccag tcgaacatcg cgcccatgcg
     1381 cttcaactgc ccgcgcatgc ggtcgatgtt tgccatcgtc cagtccatcg ggtgcttcga
     1441 accgcctttg atggcggcgt tctccgccgg cagcccgaac gcatcgaacc cgattgggaa
     1501 cagcacgttg tgtccgcgca tccgccgata gcgcgccagc gcgtccgagg gcgccatcgc
     1561 gaaccagtgc ccgatgtgca ggtcccccga ggtgtacggg aacatcgtca gcgcgtacca
     1621 gttctcacgg cccggcacgt ggtcggggat gcggtacaac ccgctctccg cccaccgctc
     1681 ctgccaacgc cgctcgacgt cctgcggctg gtagcgctcg gcctcggttc gttcctgtgt
     1741 cgtcatgtca cgaatctcgc ttcgccatcc gacctcccga ggaggccgcg tgtgtgtcct
     1801 gaaagcagtg aggaagggga cggcgacgct gccggggcag gccgcctagg taaggacgag
     1861 ggtccagcga ccccgcgccg tccggggagc agagggtcca acgcgatgca tgacgacagg
     1921 ataccggaac gcgccttcgc cgagtaccgg gcgcgggccg ccgctacagc gccaccagga
     1981 tgaggatcgc gagcaatggc ccccccgccg acaccagcag tagcagccgg cgcgcttcgc
     2041 gcagccgggc cgccgggtcc gaggtgccga ccgcccgtcg catctgccag gtcacgagga
     2101 agaagtacgc gacgaacgcc gcgatgatca cccacatgcg ccgcatccta gcgccgcgtg
     2161 accgcaggcg cgacgagacc tacggccccg ctcgcagccg caatacggct ccgccgtgca
     2221 ccagcaggta cacctcgcca gcctcgtcct ctgcgaacga ggcgatccgc cgctccgagt
     2281 cgtcacggaa caactcccgc tgctcgccgc ccgcggccgg gaccgcccac acccgcccgc
     2341 tgcagaagtc cgcgtacagg aaccacccca cgagggccgg cagctcgcgt ccgcgataca
     2401 cgaccccgcc cgtgatcgag cacccctcgt cgtgggtgta cgtcgccact ggcatcgtaa
     2461 tcccgctgcg gtcacacccg gttcgaggcg agtagcagcg atcgccctcc atccggttcc
     2521 acccgtagtt cgatccgcgc tggacgacgt cgacctcttc gacgctgctc gcaccaacat
     2581 ccccgaccca gagccgcccg tccgtgcggt cgaacgccat ccgccacggg ttccgcaggc
     2641 cgtacgccca cacctccgga cgcgcacccg cttgccccgc gaacgggttg tcccgaggca
     2701 cttcatatgg ccgagccgcg ctcgcgtttc gcacatcgat gcggacgatc ttcgccagca
     2761 gtaccccgag gttctgcccg ttcccgctcg gatcgccttc ggaacccccg tcgccgatcc
     2821 cgaggtacaa catgttgtcc ggcccgaacc ggaccgcgcc gccgttgtgg ttcgagaacg
     2881 gctgcggctg ctccatgatc acgagcttgc tgccggcgtc ggcccgatcg ttcgccacct
     2941 caaaccgcga cagcaccgtc cggcgcgggt tcgccgctga gtagtacgcg tacaggtacg
     3001 gcctggcggg gaacgccggg tcgagcgcca cgctcagcag cccctcctcg ttccccccgc
     3061 gcaacacctc gccgcggagg tcgagcagca cccccgcgtc cgacccatcg ctccgcaaca
     3121 acgacacgag ccccgcctgg tccgcgagga agaaccgccc accgggatac accccg
//
LOCUS       DAORPG010000166         3160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2961_length_3160_cov_3.284702, whole genome shotgun sequence.
ACCESSION   DAORPG010000166 DAORPG010000000
VERSION     DAORPG010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2961_length_3160_cov_3.284702"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PLX85_04745"
     CDS             <1..787
                     /locus_tag="PLX85_04745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRC62511.1"
                     /translation="ARTEAATAAATARLREQFLGITAHELRTPLTVVSGYASMLQRGL
                     ERDTLQEPVLRGAVAEIATHASRLDRLINDLLEATRVHEGRLTLNTEPMDLSELARDV
                     ARRFSEGPEASPSHRLVVEAPEPVRGEWDLARLDQVLSNLVSNAIKYSPGGGEITIRV
                     RSRAGGGAALSVQDEGVGIPARAQDELFQPFSRLVGSSDPGGIGLGLFLASQFVRRHG
                     GTISVRSEVDHGSTFTVELPSSARLDEEVAASAAPAAGARQRD"
     gene            858..2126
                     /locus_tag="PLX85_04750"
     CDS             858..2126
                     /locus_tag="PLX85_04750"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC62512.1"
                     /translation="MAEATAGDAGVRPYRWLLIGALVLCSTSMLWYGFTIGVLLPDIS
                     DDLGLRPAQEGWLSSSFYFGQLVLTLPVTAWLSRYQPLRTMGFVYVGAIVLLAAAAVT
                     PWYWVQVGIRFVLAFAFVALNPVRTLIIAGWFRRDEVARVNSVFNSGFGVVQTVAFWS
                     TGALLGVLGGWREVLWMLVGVATLSSVAWFVVAARAPRPAYALPSGGAEDSNGGGMGR
                     VLRRKQVWFLCLIGIGGALTWATYLTFWPSFAQDEMGVSKSVTGIVLGVSALTIIPGS
                     LAAVWVMRLMRSRRLFLVVATASQVPLFGLWALVDQPVLLVIIGLVQGFSWIYFPIML
                     SIPFDFEGFDARDIALASAFFIVANSLALTLGPAVSGVVAEQVEMRTVLLVAAIMPLI
                     SVLGAVLLADPPPGPMREVGGTETALASAS"
     gene            2165..>3160
                     /locus_tag="PLX85_04755"
     CDS             2165..>3160
                     /locus_tag="PLX85_04755"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRC62513.1"
                     /translation="MAGALDGIRVLEFSLIVAGPMCGLVLADMGADVIKVEPPGGEPQ
                     RKNRSVVPGESKLFQSFNHGKRDLVVDLSRPEGRAIIHRLMPSIDVVIVNYRYGVAAQ
                     LGIDYETLRGIRPDVIYVESTGWGSRGPLAQRGCSDVVAQAYSGLMAADGKLEPDGAP
                     AQVTATAIADYMTALASATAICGALFHRGRTGEGQKINTSLLRSAIWGQAHTVNREPV
                     TDSLFADVTVQKMNEIRASGGSYSDLIAMREGERSMGAQFALYYSGYQAKDGGLILGA
                     LTPANRDAFRRALELVGEDSDDPNYDALDPENVRKGDEFKEIIRQKMRTRTVAEWL"
BASE COUNT          453 a          929 c         1249 g          529 t
ORIGIN      
        1 ggcgcgcacg gaggcggcca ccgcagcggc gacggcccgg ctgcgcgagc agttcctcgg
       61 gatcacggca cacgagttgc ggacgccgct gacggtggtg agcgggtacg catcgatgct
      121 acagcgtggg cttgagcggg acacattgca ggagccggtg ctgcggggcg ccgttgcgga
      181 gatcgcgacg cacgcctcga ggctcgaccg gctgatcaac gacctgctcg aggccacccg
      241 tgtgcacgag ggacggctca cgctgaacac ggagccgatg gatctctcgg agctggcgcg
      301 agacgtggcg cgacggttct cggaagggcc ggaggcgagc ccctcgcatc ggctggtcgt
      361 ggaggcgccc gagccggtgc gcggcgagtg ggacctcgcc cgcctcgacc aggtgctgag
      421 caacctcgtg agcaacgcca tcaagtattc ccccgggggc ggcgagatca cgattcgggt
      481 gcggtctcgg gcgggtggcg gagcggcgct cagcgtccag gacgagggcg tcggcattcc
      541 ggcgcgcgcg caagatgagc tgttccagcc cttcagccgg ttggtaggga gttcggatcc
      601 gggcggtatc ggcctcgggc tgttcctggc ctcgcaattc gtgcgccggc acggggggac
      661 gatcagcgtg cgcagcgagg tggaccacgg cagcacgttc acagtggagc tgccatcgag
      721 cgcgcgactg gacgaagagg tggcggcgag cgccgcgccc gcggcagggg cgcgccagcg
      781 cgattgacgc gtggccgcag atggactacc aaacgtgatg ccctcccgag tcggcgagcg
      841 gcatcgaggg ggcgcgcgtg gcagaggcga cagcggggga cgcgggcgtc aggccgtacc
      901 ggtggctgct gatcggggcg ctcgtcctct gctcgacatc gatgctctgg tacgggttca
      961 cgattggtgt gctgctgccg gacatcagcg acgacctcgg actgcggccg gcgcaggagg
     1021 ggtggctgag ctcctcgttc tacttcgggc agctcgtgct gacgctcccg gtgacggcat
     1081 ggctatcgag gtatcagccg ctgcggacga tgggcttcgt gtacgtcggg gcaatcgtgt
     1141 tactcgcggc ggcggccgtg acgccgtggt actgggtgca ggtggggatc cgcttcgtcc
     1201 tcgcgttcgc gttcgttgcg ctgaaccccg tgcgtacgtt gatcatcgcg gggtggttcc
     1261 ggcgcgacga ggtggcgcgg gtcaacagcg tgttcaactc ggggtttggc gtggtgcaga
     1321 cggtcgcgtt ctggagcacc ggggcgctgc tgggggtgct gggtggctgg cgtgaggtgc
     1381 tgtggatgct cgtcggcgtg gcgacgctgt cttcggtggc gtggttcgtg gtggcggcac
     1441 gggcgccacg gccggcgtac gcgctgccct cgggcggcgc tgaagacagc aatggcggcg
     1501 ggatgggccg cgtgctgcga cggaagcaag tgtggttcct gtgtctgatc ggtatcggtg
     1561 gggcgctgac gtgggcgacc tacctgacgt tctggccctc gttcgcgcag gacgagatgg
     1621 gcgtgtccaa gagcgtgacg ggtatcgtgc tcggcgtctc ggcgctcacg atcatccccg
     1681 ggtcgctggc ggcggtgtgg gtgatgcggc tgatgcgcag ccggcgcctg ttcctcgtgg
     1741 tggcgacggc gtcgcaagtg ccgctgttcg ggctgtgggc gctcgttgac cagccggtgt
     1801 tgctggtcat catcgggctc gtgcaggggt tcagctggat ctacttcccg atcatgctga
     1861 gcatcccgtt cgacttcgag gggttcgacg cgcgggatat cgcattggcc tcggcgttct
     1921 tcatcgtggc gaactcgctc gcgttgacgc tcggtccggc ggtgtcaggc gtcgtcgccg
     1981 agcaggtcga gatgcgcacg gtcctgctgg tggcggcgat catgccgctg atctcagtgc
     2041 tcggggcggt gctgctcgcg gacccgccgc ccgggccgat gcgcgaggtg ggcgggacgg
     2101 agaccgcgct cgcatcggcc tcgtagagtc cgactcagac gcgggcgact ggagggggtg
     2161 aacgatggcc ggagcgctcg atgggattcg cgtcctcgaa ttctcgctga tcgtggcggg
     2221 gccgatgtgc gggctggtgc tcgccgacat gggcgcggac gtgatcaagg tggaaccgcc
     2281 cgggggcgag ccgcagcgca agaaccgcag cgtggtgccg ggcgagagca agctctttca
     2341 gtcgttcaac catggcaagc gcgacctcgt cgtcgacctc tcgaggccgg aggggcgcgc
     2401 gatcattcac cggctgatgc cgagcatcga cgtggtgatc gtgaactacc ggtacggcgt
     2461 ggcggcgcaa ctcggcatcg attacgagac gctgcgaggc atccgcccgg acgtcatcta
     2521 cgtcgaaagc acgggctggg ggtcgcgggg cccgctggcg cagcgcggct gctcggacgt
     2581 ggtggcgcag gcctattcgg ggctgatggc ggcggacggg aagctcgagc cggatggagc
     2641 gccggcccag gtgacggcga cggcgattgc ggactacatg accgcgctgg cctcggcgac
     2701 cgcgatctgt ggagcgctgt tccatcgagg ccgcaccggc gaggggcaga agatcaacac
     2761 ctcgctgctg cggtcggcga tctggggaca ggcgcacacg gtaaaccgcg agccggtgac
     2821 ggactcgctg ttcgcggacg tgaccgtcca gaagatgaac gagattcgtg cgtcgggggg
     2881 gtcgtactcg gacctgatcg cgatgcgcga gggcgagcgc tcgatgggcg cgcagttcgc
     2941 gctctactac agcggctacc aggcgaagga cggcggattg atcctcggag cgctgacgcc
     3001 ggcgaaccgg gacgcgtttc ggcgggcgct ggagctcgtg ggcgaggaca gcgatgaccc
     3061 gaactatgat gcgctcgacc cggagaacgt gcgcaagggt gacgagttca aagagatcat
     3121 tcgccagaag atgcggacgc ggacggtggc ggagtggctc
//
LOCUS       DAORPG010000167         3155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2968_length_3155_cov_3.191290, whole genome shotgun sequence.
ACCESSION   DAORPG010000167 DAORPG010000000
VERSION     DAORPG010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2968_length_3155_cov_3.191290"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PLX85_04760"
     CDS             complement(<1..360)
                     /locus_tag="PLX85_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62514.1"
                     /translation="MLELVVALLIGTIAVGIVLAMGRDARGGRYPSRPRGRGRGVPVE
                     PRHAAVDPRDEWVDEEYEVPYEAPRRRARELAGAAPADAADRGGGRSWLILVWAGLGV
                     FAAVLFVAAARSGGGGSA"
     gene            complement(469..1602)
                     /locus_tag="PLX85_04765"
     CDS             complement(469..1602)
                     /locus_tag="PLX85_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62515.1"
                     /translation="MILNRPLQEWQANAFAEAGLARSETPEPPCLIVPDTLFASAGAL
                     RAFIDGAAGEDAVLVLKESEFGRWTTAVQPGVSQVGEGYVFDAIRFVSGSQRPPRRVV
                     VDPEERVIDLGTIDPYTGGASSKIGLPRHPVMTLHHWVHILWANQVAGASEAWAAPRW
                     KQGARVLGAIVRSHSLNRWRVLAKLSSRGRGCDVHPSALVEGSTLGDGVTVGPNARVL
                     FSRVGDGATVMAGAQVELSVLGERSLVAQNTLVRLCVLYPEATVGLGAQQSVFGRNAV
                     TTGASVLDMNFEQPVRVELDGQLHSSGQHFLGSAFGHRSRLGAGFFLAPGRAVPNDYL
                     LIRSPERVLATLPPDLAEAGPLVADERVLRPLHATPTAFRPEK"
     gene            1924..2268
                     /locus_tag="PLX85_04770"
     CDS             1924..2268
                     /locus_tag="PLX85_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRC62516.1"
                     /translation="MTAEEPAAETRGITVQHLASLDLGAEIEGLAGRTLRMRMVTIEP
                     GGVFGPLHDHVDRPGMVYVLQGTITDHRDGTTTDYGPGPGWPEDRKTRHWLENRGTIA
                     AVEISIDIVAER"
     gene            2366..>3155
                     /locus_tag="PLX85_04775"
     CDS             2366..>3155
                     /locus_tag="PLX85_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62517.1"
                     /translation="MPTSVGQAIVTIDADYRHALIGADDARAQMSFALFKMMDSGRPF
                     YSWDVLHHYFADIYQPQGIGMIPWTVVRGFSNDSAREEGRQAGIYSTAVQGCVLVDLE
                     DDPRLFWQGIESAVDAWIQGFVEGGGREVFLWPDSRADKLKGENFWRWWHYKIVTRVW
                     PQAYFSIFFGPGVRNRVRRGIDRAVQPLVDGGVPMSAITPTLMTSDNDGNPIPWGELL
                     EGIHYCRELGSSGFALWRRQFLSPEQRDGLAALDDPFAPPPSRRP"
BASE COUNT          518 a         1208 c          963 g          466 t
ORIGIN      
        1 tgccgatccg ccgcccccgg agcgcgcggc cgcaacgaac aggactgctg cgaacactcc
       61 cagccctgcc cacaccagga tcagccagga cctgcctccg ccgcggtccg ccgcgtcggc
      121 gggcgccgcg cccgccagtt cacgcgcacg ccgccgaggt gcctcgtatg ggacctcgta
      181 ctcctcgtcc acccattcgt ctcgaggatc caccgccgcg tgtcgcggct cgaccggcac
      241 tccgcggccc cgccctcgcg gcctgctcgg gtagcggccg ccgcgcgcgt cgcgtcccat
      301 cgcgaggaca atgcccaccg cgatcgtccc gatcagcagc gcaaccacca gctccagcat
      361 cgaccgcgcc ttcgaggaca cgcttgcttg gcgtgatcga tcgtacccgc ggtgcggtac
      421 cccctgcgcg ttgtcgttgc gtagtgcagc ccaacgagga aacccagttc acttctcggg
      481 gcgaaacgcg gtcggtgtgg cgtgcagcgg gcgcagcacg cgctcgtccg ccacaagtgg
      541 tccggcctcg gctaggtcgg gcggaagtgt ggcgagcacc cgctcggggc ttcggatcag
      601 taagtaatcg ttcggcaccg cccgaccggg tgccagaaag aagccggccc cgagccggct
      661 ccggtgcccg aatgccgatc ccaggaagtg ctgccccgac gagtgcagct gcccgtccaa
      721 ttccacgcgc accggctgct cgaagttcat gtcgagcacg gacgcgcccg tagtcaccgc
      781 gttccgcccg aacacggact gctgcgctcc cagcccgaca gtcgcctcgg ggtacagcac
      841 gcacagccgg accagcgtgt tctgcgccac aagtgaccgc tcgccgagca cgctgagttc
      901 gacctgcgca ccggccataa cggtggctcc atcgcccacc cggctgaaga gcacacgagc
      961 attgggcccc accgtgacgc cgtccccgag cgtcgacccc tcgacgaggg cgctgggatg
     1021 aacgtcgcac cccctgccgc ggctgctcag cttggcgagc actctccacc ggttcaacga
     1081 atgcgaacgc acgatagcgc cgaggacgcg cgcaccctgc ttccaacgag gtgccgccca
     1141 cgcctccgag gcgccagcca cctggttcgc ccacagaatg tgcacccagt ggtggagagt
     1201 catcaccggg tggcgcggaa ggccgatctt cgatgacgcc cctcccgtat acgggtcgat
     1261 ggtgccgagg tcgatcaccc gctcttcggg atcgacaacc acgcggcgag gtggccgctg
     1321 tgagccgctg acaaaccgga tcgcgtcgaa gacgtacccc tcgcccacct ggctgacccc
     1381 gggctggacc gccgtggtcc acctgccgaa ctcagactcc ttcaggacca ggaccgcgtc
     1441 ctcgccggcc gcgccatcga tgaacgcacg aagagcaccc gccgaagcga acagcgtgtc
     1501 cgggacaatg aggcacggcg gctctggcgt ctcgctgcgc gccagccccg cctcagcaaa
     1561 cgcgttggcc tgccattcct gcagcgggcg attgaggatc aatgtcctct ccacggcatc
     1621 gccaaacggc gcgatccgcc tcccggtcgc ttcaacaaac actcgggtac gttccacgcg
     1681 tactcccacc gtgtccgacc cggcggcgag ccggccgtgg cggtcggctg gaaccggcgc
     1741 tgtggcagga cgctaccgcg tgagggagac tcggcacaag cgcgtctgag cacgccccgt
     1801 tccttcgact ggcgcccgcc ctgacctgat cctcagccgt cacagccccc cgtgcgcccg
     1861 ctggtagcct gagcatcgaa gccgcgccgc gcccccgccc gcgcgagaga aaggagttcc
     1921 gccatgaccg ccgaggaacc agcggccgag actagaggca tcaccgtcca gcacctggca
     1981 tcgctcgacc tcggggcgga aatcgagggc ctggccggcc gcacgctccg catgcgcatg
     2041 gtcacgatcg agcccggcgg cgtcttcggc cccctccacg accacgtcga ccgccccggc
     2101 atggtctacg tcctgcaggg cacgatcacc gaccaccgcg atgggaccac caccgattac
     2161 ggcccagggc cgggctggcc ggaggatcgg aaaacaaggc actggctgga gaaccgagga
     2221 acgatagctg ccgtcgagat ctcgatcgac atcgtcgccg agcgttgaag aggtgtcggc
     2281 tggccccccg cggctgccgt tccccccttc tcgccacttg cacgaccgtg ataatcatga
     2341 ccacggttaa cgattggagc gcttcatgcc caccagtgtc ggacaggcca tcgtcaccat
     2401 cgacgccgac taccgccacg cactcatcgg cgccgacgac gctcgcgccc agatgtcgtt
     2461 tgccctcttc aagatgatgg actccgggcg tccgttctat tcgtgggacg tcctgcacca
     2521 ctacttcgcg gacatctacc agccgcaggg cattggcatg atcccctgga ccgtcgtccg
     2581 agggttctcg aacgactccg cccgcgaaga aggccgccag gccggcatct actccaccgc
     2641 cgtccagggc tgcgtgctcg tcgatctcga agacgaccct cgcctcttct ggcagggcat
     2701 cgagtcagcc gtcgacgcct ggatccaggg cttcgtagag gggggcggcc gcgaggtctt
     2761 tctctggccc gacagccggg ccgacaagct gaagggagag aacttctggc gctggtggca
     2821 ctacaagatc gtcacccgcg tctggccgca ggcctacttc tcgatcttct tcggccccgg
     2881 cgtccgcaac cgtgtccgcc ggggcatcga ccgcgccgtc cagccgctgg tcgacggcgg
     2941 cgtcccgatg tccgccatca cgcccacgct catgacctcc gacaacgacg gcaacccgat
     3001 cccctggggc gagctcctcg agggcatcca ctactgccga gagctgggct ccagcggctt
     3061 cgccctctgg cgtcgccagt tcctgtcgcc cgaacagcgc gacggcctcg ccgccctcga
     3121 cgatcccttc gccccgcccc cgagccgccg ccccc
//
LOCUS       DAORPG010000168         3154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2972_length_3154_cov_3.088093, whole genome shotgun sequence.
ACCESSION   DAORPG010000168 DAORPG010000000
VERSION     DAORPG010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2972_length_3154_cov_3.088093"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PLX85_04780"
     CDS             complement(<1..270)
                     /locus_tag="PLX85_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRC62518.1"
                     /translation="MSEVTAAILLAAGSSQRMGGVDKLWYEVEGLPLVGYALRTLAEL
                     DEVNVVVAVAPRERHEALRGLVGDVEGVELRCVEGGARRQDSVAAG"
     gene            complement(273..1589)
                     /locus_tag="PLX85_04785"
     CDS             complement(273..1589)
                     /locus_tag="PLX85_04785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC62519.1"
                     /translation="MTTSVETPRRRGLFYGWYIAIAGATNNALILGVTVFGFGVFIEP
                     VRKDLGWSLAALTIGFSIRSFQSGLLAPFVGLLLEWIGPRRMSIVGVFVVAGGLLFMA
                     QMHALWEFYVSSMVVALGQSMGGVTPFSTTIVRWFRRSRGRAMGVLFSGNALGYGMAP
                     VMAFFIVHYGWRVALVVAASAIVLVGTPLAFVIRPRPEDHGLLPDGDLAPSEAAAPGT
                     ALEESGMTVAETVRTPAFYLLMLAMTVGAPTQSALIVFQVPVLEHAGYSAGVAALLAA
                     GYGLVQAPVRLVVGGIADRIGRRRAYAWCFLLQAAGLVVFASMDSSRVWLVPLFYGTY
                     ALGHALFTTLFLTTVADYFGTRRFASLQGIIQFAGMPVGLALPIVTGVVFDRTGTYST
                     IFLVYAALASLGAVFVFLIRRPTWHEFSSALTAAPPATAAAAAAGD"
     gene            1716..2222
                     /locus_tag="PLX85_04790"
     CDS             1716..2222
                     /locus_tag="PLX85_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRC62520.1"
                     /translation="MRRGGIRARLLAGVLLLVAALMLGLAACGDDDHTPPEVTRPDEV
                     GQQLVTKYMTLLQQKDTAQLRTFLSDAFLIQRADGSWSTKDEYLKNLPEIREYTVQSI
                     TAKQDGPVLTVKWALEVDQTINGQLYRGDPAPRLSSFVWDGAEWHLASHANFNTPATA
                     TPTPASGR"
     gene            complement(2244..2606)
                     /locus_tag="PLX85_04795"
     CDS             complement(2244..2606)
                     /locus_tag="PLX85_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HRC62521.1"
                     /translation="MDRARFRRLVQRALRSLPPEVRDALDNVAVVIEREPSPEELRRA
                     GVEAGDTLFGLYVGTPLTERAGYGLTLPDRIVIYQGPLERHFHPRDIPREVATTVRHE
                     LGHHFGIEDDRLDVLGLG"
     gene            complement(2657..>3154)
                     /locus_tag="PLX85_04800"
     CDS             complement(2657..>3154)
                     /locus_tag="PLX85_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62522.1"
                     /translation="RIAASTQTQRLTCPSGAETTAELWMIEPCQGRAPGSDVEVYEFH
                     TGKGPVFFGDREAYIAAVFQGLSPGGSDIVMLAVGCGVPKSVPASSADCATEGVAVYR
                     NLRYSSEVALVFSRRSDTDRWMIEKFWSIFPSTVPETIPGGLRLEINTIAGVSAVELY
                     PYSPE"
BASE COUNT          568 a         1179 c          991 g          416 t
ORIGIN      
        1 gcccgccgcc accgagtcct gccgccgcgc gccaccctcg acgcagcgca gctcgacgcc
       61 ctcgacatcc ccgaccagcc ctcgcagcgc ctcgtgccgt tcccgcggcg cgaccgccac
      121 cacgacgttc acctcgtcca gctcggccag cgtgcgcagc gcgtacccga cgagtggcaa
      181 cccctcgacc tcataccaga gcttgtcgac gccgcccatc cgctgcgagg aacccgcggc
      241 gaggaggatc gcagccgtga cctcggacat cgtcaatcgc ccgcggccgc cgcagcggcg
      301 gtagccggcg gggcggctgt gagcgccgag ctgaactcat gccaggtcgg ccgccggatg
      361 aggaacacga acaccgcccc gaggctcgcg agcgccgcat agacgaggaa gatcgtcgag
      421 tacgtccccg tgcggtcgaa gacgacgccc gtgacgatcg gcagcgccag ccccaccggc
      481 atccccgcaa actggatgat cccctgcaac gaggcgaacc gccgcgtccc gaagtagtcc
      541 gccaccgtcg tcaggaacag cgtcgtgaac agcgcgtgcc ccagcgcgta cgtcccgtag
      601 aacaagggca cgagccacac ccgcgaggag tccatgctcg cgaacaccac gaggcccgcc
      661 gcctgcagca ggaagcacca ggcgtaggcg cggcgtcgcc cgatgcggtc cgcgatcccg
      721 cccaccacga gacgcaccgg cgcctgtacc aggccgtacc ccgccgccag tagcgccgcc
      781 accccggcgg agtaccccgc gtgctccagc accggcacct ggaacacgat cagcgccgac
      841 tgcgtgggcg cgcccacggt catcgcgagc atgagcagat agaacgcggg cgtccgcacg
      901 gtctcggcca cggtcatgcc ggactcctcg agcgccgtcc ccggcgccgc cgcctccgag
      961 ggcgccaggt cgccgtccgg cagcagcccg tggtcctcgg ggcgcggacg gatcacgaag
     1021 gcgagcggcg tccccacgag gacgatcgcg ctcgccgcca cgaccagcgc cacgcgccag
     1081 ccgtagtgca cgatgaagaa cgccatcacc ggcgccatgc cgtagccaag cgcgttcccg
     1141 gagaacagca cgcccatcgc gcggcctcgc gagcgccgga accagcgcac gatcgtcgtc
     1201 gagaagggtg tgacgccgcc catgctctgt ccgagcgcaa cgaccatgct cgagacgtag
     1261 aactcccaca gcgcgtgcat ttgcgccatg aacagcagcc cgccggccac gacgaacacc
     1321 ccgacgatgc tcatccgccg tggcccgatc cactcgagca gcagccccac gaacggagcg
     1381 agcagtcccg actggaacga ccggatcgag aacccgatcg tcagcgccgc cagcgaccac
     1441 ccgaggtcct tgcgcaccgg ctcgatgaac acgccgaacc cgaagacggt gacgccgagg
     1501 atgagcgcat tgttggtcgc cccggcgatg gcgatgtacc acccgtagaa cagccctcga
     1561 cggcgcggcg tctcgacgga cgtggtcacg cgcgcatact acgcgaccac cgtccgggcg
     1621 tccggccgac ccgcgaggcc gcgtaaggtc gtctcagcgc tgcggccgtg gtagatcact
     1681 ccgaggcaac aacatgcgcg ccgaggaggc tgacgatgcg tcgaggtggt attcgagcca
     1741 ggctgctggc cggcgtcctc ttgctcgtcg ctgcgctcat gctcgggctc gccgcctgcg
     1801 gcgacgacga ccacacgccc cccgaggtca cacgccctga cgaggtcgga cagcagctgg
     1861 tcacgaagta catgacgctg ctgcagcaga aggacaccgc ccagctgcgc accttcctct
     1921 ccgacgcctt cctcatccag cgcgccgacg gcagctggtc gacgaaagac gagtacctca
     1981 agaacctgcc cgagatccgc gagtacacgg tccagagcat caccgcgaag caggacggcc
     2041 ccgtcctcac cgtgaaatgg gcgctcgagg tcgaccagac gatcaacggg cagctctacc
     2101 gcggcgatcc cgcgccccgg ctgtcctcgt tcgtctggga cggggccgag tggcatctcg
     2161 cgtcgcacgc caacttcaac acgcccgcga cggcgacgcc cacgccggcg tccggccgct
     2221 gagctgcgac tcgacgggat ccgtcagccg agtccgagga cgtccagccg gtcgtcctcg
     2281 atcccgaagt ggtggcccag ttcgtggcgc accgtcgtcg ccacttcccg cggaatgtcg
     2341 cgaggatgga agtgccgttc gagggggccc tggtagatca cgatgcggtc cggcagcgtc
     2401 agcccgtacc ccgcgcgttc ggtgagcggc gtccccacgt agagcccgaa cagcgtgtca
     2461 cccgcctcga cgccggcgcg ccgcagctcc tcgggactcg gttcgcgctc gatcacgaca
     2521 gccacgttgt cgagggcgtc tcgcacctcg ggaggcagtg agcgcagtgc ccgctgcacg
     2581 agccgtcgaa accgcgcgcg gtccatgccc gcgagtctac aatcgccgag ccgctcgctc
     2641 tcaccagcgg ccgccatcac tccggcgagt acggataaag ttcgaccgcg gaaacgccgg
     2701 cgattgtatt gatttcaagg cgcaacccgc ccgggatggt ctcgggcacc gtgctcggga
     2761 agatgctcca gaacttctcg atcatccacc ggtcggtatc cgatcgacgg ctaaagacga
     2821 gtgcgacctc ggatgaatat ctcaggttgc ggtacaccgc gacgccttcc gtggcgcagt
     2881 cggccgagct tgccggtacg gacttcggaa caccgcaccc caccgctagc atgacgatgt
     2941 cggagcctcc cggagacagg ccctggaata cagcggcgat gtaggcttcg cgatctccga
     3001 aaaacacggg gcccttcccc gtgtgaaatt cgtacacctc gacatctgag ccgggtgctc
     3061 tgccctgaca gggctcaatc atccacaact ccgcggttgt ttctgccccg ctaggacatg
     3121 tcaggcgctg tgtctgcgtg gaggccgcga tacg
//
LOCUS       DAORPG010000169         3141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_2996_length_3141_cov_3.238496, whole genome shotgun sequence.
ACCESSION   DAORPG010000169 DAORPG010000000
VERSION     DAORPG010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2996_length_3141_cov_3.238496"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..1612
                     /locus_tag="PLX85_04805"
     CDS             209..1612
                     /locus_tag="PLX85_04805"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HRC62523.1"
                     /translation="MAPGFGPRERGALLAGRAAGQVVRRLGRGGGTALPGLIAGRLAP
                     TLIESAAAQLGHGVVTVTGTNGKTTTAHLLAAIARGAGLRPLANRSGSNLERGIVSSL
                     VDAVDPGGSVHDAARRLGVFEIDEAHWPLLAPRLRPRVALFLNLFRDQLDRYGEVDSI
                     AAAWRAAVSRPGAVPTLVLNVDDPSVAQLGEDTRGEVIGFGIEDPAAALPAPEHATDA
                     RFCRCGATYEYDSIYMGHVGLWRCRGCERRRPAPTVSAERVHLGAEETQFDLVIGERR
                     VPVAIPLAGLYSVYNALAAAAGAHALGLPIEATARALADSGPAFGRQERFHVEGRTVR
                     LLLAKNPTGLNEVLRALEAAWPPLTLLAMLNDGIQDGRDVSWIYDADVERLAGLVGKL
                     VVSGRRAEDLALRFALAGIEPAAVDTDTRVALQTALLATPPGGRLEVVATYTAMLEVR
                     ELLASMSGAGHYWEHDE"
     gene            1609..2373
                     /locus_tag="PLX85_04810"
     CDS             1609..2373
                     /locus_tag="PLX85_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006547974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HRC62524.1"
                     /translation="MNDLARTIRVVTLYPDVMSIYADRGNLIALRARCRQYGIGCEVT
                     AVGIGDGLPADADLVLIGGGQDQEQRRIAADLAARGVRLRDWAEDDVAMLTICGGFQL
                     FGHFYRTAEGEELPGVGIFDVTTVAPAPGAPRCIGDVLADAGDFGEVVGFENHGGRTY
                     LGPHARPFARMRHGFGNNGDDGTEGAVYREAIGTYLHGSLLPKNPAVTDRLILAALRH
                     RYGPGVMLETLPPDPYAERAHRAAAAVARRRGRPNP"
     gene            2440..>3141
                     /locus_tag="PLX85_04815"
     CDS             2440..>3141
                     /locus_tag="PLX85_04815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRC62525.1"
                     /translation="MLTRRRLLVLGLAVPFAAACGRGGSEGSDGGVEVGTPVLQRTPT
                     PASTTASRTPVATATPAPTGRLRLDDLTGFAVPVAGACLPDSDNLMPNAPRTYRAGIH
                     EGVDFYPGLACAPIAKGTPVLAMFEGTVIRSDLNYEELTPALLAKLRADTAAAGTTPP
                     AALDAYRGRQVWVDHGQGVVTRYCHLSSIAAGIAVGTRVRQGQQVGGVGESGTPESIE
                     APGTELHLHAEVRLGD"
BASE COUNT          413 a         1254 c         1007 g          467 t
ORIGIN      
        1 ccagccgctc cgactcgccc gatgacgaaa agaagaagga ctccgacaag cccgccgcga
       61 cctccgggga gtcccgctcc agctgacccc cgggcgccgg ccgccgaagg gggaagcccc
      121 cgaggcgatt tgcaggtcag atcatgatgg acgcgcggct caccggccgc gcgttcgtgt
      181 ttgagcaggt agagagccga gtccccgcat ggcccccggc ttcggtcccc gcgaacgagg
      241 cgccctgctc gccggtcgcg ccgccggcca ggtcgtgcgg cgcctgggtc gtggtggtgg
      301 caccgcgctt cccggcctga tcgccggccg gctcgcgccc accctcatcg agtccgccgc
      361 tgcccagctc ggccacggcg tcgtcaccgt gaccggcaca aacggcaaga ccactaccgc
      421 ccacctgctc gccgccatcg cccgaggcgc cgggctgcgc ccgctcgcca accgttcggg
      481 ctcgaacctc gagcgcggca tcgtgagctc gctcgtcgac gccgtcgatc ccggcgggag
      541 cgtccatgac gccgcgcgcc gcctcggcgt gttcgagatc gacgaggccc actggcccct
      601 gctcgcgccc cgtctgcggc cgcgcgtcgc cctcttcctc aacctctttc gtgatcaact
      661 cgaccgctac ggtgaggtcg actccatcgc cgctgcctgg cgtgcggccg tcagccgccc
      721 gggcgctgtc cccacgctcg tcctgaacgt cgacgacccc tcggtggccc agctcgggga
      781 ggacacccgt ggcgaggtga tcggcttcgg catcgaggat ccggccgctg ccctgcccgc
      841 gcccgaacac gccaccgacg cgcgcttctg ccgctgcggc gccacctatg agtacgactc
      901 gatctacatg ggccacgttg gcctctggcg ctgccgcggc tgcgagcgcc gccgcccggc
      961 ccccactgtc tccgccgaac gcgtccatct cggcgccgag gagacgcagt tcgacctcgt
     1021 catcggcgag cgccgcgtgc ccgtcgccat cccgctcgcc ggcctctact cggtctacaa
     1081 cgcgctggcg gccgccgcgg gcgcgcacgc cctcggcctc cccatcgagg cgacggcgcg
     1141 tgccctcgct gattccggtc ctgccttcgg tcggcaggag cgctttcacg ttgagggtcg
     1201 cacggtccgc ctgctcctcg ccaagaaccc gaccggcctg aacgaggtgc tgcgcgccct
     1261 cgaggcggcc tggcctccgc tgaccctcct cgcgatgctg aacgacggca tccaggacgg
     1321 tcgcgacgtt tcctggatct acgatgccga tgtggagcgg ctcgccggcc tcgtcgggaa
     1381 actcgtggtc tccggccgcc gtgccgagga cctcgcgctt cgctttgctc tcgccgggat
     1441 cgagcccgcc gccgtcgaca ccgacacccg agtcgcgctc cagacggccc tgctcgccac
     1501 gccacccggg gggcgcctcg aggtggtggc gacctacacc gccatgctcg aggtgcgcga
     1561 actcctcgca agcatgtccg gcgccggcca ttactgggag cacgacgaat gaacgacctc
     1621 gcgaggacga tccgcgtcgt caccctctac ccggacgtca tgagcatcta cgcggatcgc
     1681 ggcaacctga tcgcgctgcg cgctcgctgt cgccagtacg gcatcggctg cgaggtgacc
     1741 gctgtcggca tcggtgacgg cctcccggcg gacgccgacc ttgtgctcat cggcggtggc
     1801 caggaccagg agcagcgccg cattgccgcc gacctcgccg cacggggcgt ccgcctccgc
     1861 gactgggccg aggacgacgt cgcgatgctc accatctgcg gcggcttcca gctcttcggc
     1921 catttctacc gcaccgccga gggcgaagag ctgcccggtg tcggcatttt tgatgtcacg
     1981 accgtggccc ccgcccccgg cgcgcctcgc tgcatcggcg atgtcctcgc cgacgcgggc
     2041 gatttcggcg aggtcgtcgg cttcgagaac cacggcgggc gcacctacct cggtccccac
     2101 gcccggccct tcgcacgcat gcgtcatggc ttcggcaaca acggcgacga cggcaccgag
     2161 ggcgccgtct accgcgaggc gatcggcacg tacctccatg gctcgctgct cccgaagaac
     2221 cccgcggtca ccgaccgcct gatcctcgcc gcgctgcggc accgctatgg gccgggcgta
     2281 atgctcgaga ccctgccgcc tgacccgtac gccgaacgcg cgcatcgcgc ggcggctgcc
     2341 gttgcgcgtc gccgcggccg tcccaacccc tagctcgcga acctcgagga tcgacacctg
     2401 gtttgacggc aggcctcgcg cctcccaatc ctgatccgca tgcttacgcg ccgccgcctg
     2461 ctggtcctcg gcctcgctgt gcccttcgcc gcggcctgtg gtcgcggggg ctccgagggc
     2521 tcagacggcg gagtcgaagt tggcacgcct gtcctccagc gcacgccgac tccggcgagc
     2581 accacggcga gccgcacgcc ggtggccact gccaccccgg caccaacggg caggctccgc
     2641 ctcgacgatc tcaccggctt cgccgttcct gtcgccggcg cctgtctacc cgacagcgac
     2701 aatctgatgc cgaacgcccc ccgtacctac cgtgccggca tccacgaggg cgtcgacttc
     2761 taccccggcc tcgcctgcgc gccgatcgcg aagggcaccc ccgtcctcgc gatgttcgag
     2821 gggaccgtga tccgctctga cctcaactac gaggagctca cgcccgcgct cctcgcgaag
     2881 ctccgtgccg ataccgccgc ggccggcacc acgccacccg ccgccctcga tgcgtaccgc
     2941 ggacgccagg tctgggtcga tcacggccag ggcgtcgtca cgaggtactg ccacctctcg
     3001 tcgatcgccg ctggcatcgc cgtcggtacg cgcgtgcgcc agggccagca ggtcggcggc
     3061 gtcggcgagt ccggcacgcc tgagtcgatc gaggcgcccg gcacggagct gcacctgcac
     3121 gctgaagtgc gcctcggcga c
//
LOCUS       DAORPG010000170         3135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3007_length_3135_cov_3.151623, whole genome shotgun sequence.
ACCESSION   DAORPG010000170 DAORPG010000000
VERSION     DAORPG010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3007_length_3135_cov_3.151623"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(348..605)
                     /locus_tag="PLX85_04820"
     CDS             complement(348..605)
                     /locus_tag="PLX85_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62526.1"
                     /translation="MCTPGVAVGVAVAVGDGVAASGVPLAVATLVAITVVVAATSSRG
                     GSDGLELVAPPQRTEARTTTSTTSHASVARLLRIRACAALI"
     gene            625..1461
                     /locus_tag="PLX85_04825"
     CDS             625..1461
                     /locus_tag="PLX85_04825"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRC62527.1"
                     /translation="MVVLDPGHGGDDPGAASFGIVERESNLDMAERIGTALRERGIEV
                     VLTRATADRPRVDGRDATLAGATATFLDLQDRIAIANAAQADVFVSIHSNGCVNPDAS
                     GVEVWFDSTRSFAEANRSLAEAVLSGIEARAGTSGAAVPVHGLEDDVGVADIAGHTTP
                     LFVLGPTREVTWEELMGRGIEPSALGVTDPGASFWTDASTMPGVLVELLYVTNPSDAA
                     LLANDDARSAMATGVADGIARYLGQLAANENRSASAAGGSSVANRTVASAPRPPAGCA
                     PR"
     gene            complement(1514..2002)
                     /locus_tag="PLX85_04830"
     CDS             complement(1514..2002)
                     /locus_tag="PLX85_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC62528.1"
                     /translation="MFTINRLNHAVLWVRDANAALDFYRDVLGFTVVESMGDRAHFLR
                     ANGSDNHHDLGLFGIGAAAPPPTRGERVGLYHLAWEVDTLPDLARARVDLANAGALTG
                     QSDHGSSLSLYGEDPDGNQFEVFWMVPREEWETRGFGTRPLDLEGELRRRGFDPIEAS
                     TS"
     gene            complement(2246..2797)
                     /locus_tag="PLX85_04835"
     CDS             complement(2246..2797)
                     /locus_tag="PLX85_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRC62529.1"
                     /translation="MTTRPAVQTATYTIDAAHSSVEFAVKHMMVASTRGRFTDLEGSI
                     HLDEADPTRSSVEAVIRTASVNTGVEFRDNDLRSDNFFDAERYPEIRFRSTSVEPGKD
                     DRWLVHGDLTIRDVTRPVTLDTELEGRGTGFQGEDRIGFTATTSIDRKEFGLNYNAVL
                     ETGGFVVGDRVRITLTIEAVHQP"
     gene            2902..>3135
                     /locus_tag="PLX85_04840"
     CDS             2902..>3135
                     /locus_tag="PLX85_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012786403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HRC62530.1"
                     /translation="MTTKHAEPHADLDAFCPRYHQAVELVGRRWSGAILRAMLAGGDR
                     FTELRETIPGMSDRLLSERLKEFEAEGLVERLVF"
BASE COUNT          459 a          946 c         1224 g          506 t
ORIGIN      
        1 ccttccgcgc acgaactcga tcgtagcagc ctcagatccg cgacgacgcg ccgaaccgtc
       61 ctcttgcccg cgaccctcgt ggcgctcccg gcgtgcctcg tcccaacgaa cggatgacct
      121 tcgccgtgcg gacttcgtcc cgtcgccccc gatgctggag ggcgggtggg ttctgaggga
      181 gcaccggatg gacatggagg cgtggccggc cggcctcggg cgacgagcct ggtatcggaa
      241 tctcgccgat gtcgaccgta ctgtggatgg tgtcgcggcg tcgcgctcgt cacagtgagc
      301 ggctggtaac gtcgtggcgg tgggttccgg caaggggtga gccgaggtca gatgagggcg
      361 gcgcaggctc ggatgcggag gaggcgggca accgaggcgt ggctcgtggt gctggttgtc
      421 gtcctcgcct cggtccgctg cggcggggct acgagctcga ggccgtcgga gccgccccgt
      481 gacgatgttg cagccacgac cacggtgatg gcgacgagcg ttgccaccgc cagtgggacg
      541 ccggaggcgg caacgccgtc ccccaccgcg accgcgaccc cgaccgcgac cccaggagtg
      601 cacacgcagc cgccggctcc gtgggtggtc gtcctggacc cgggacacgg cggcgacgat
      661 ccgggcgccg cgagcttcgg gatcgtggaa cgcgaatcaa acctcgacat ggcggagcgc
      721 atcggcaccg cgctgcggga gcggggaatc gaggtcgtgc tgacccgcgc gaccgcggac
      781 cggccacggg tcgatgggcg cgacgctacg ctcgcgggcg caacggcgac gttcctggac
      841 ctgcaggacc gaatcgcgat cgcgaacgcg gcgcaggccg acgtgttcgt ctcgattcac
      901 tcgaatggct gtgtgaatcc ggacgcgagc ggggtcgagg tctggttcga cagcactcgg
      961 tcattcgccg aggcgaaccg ttcgctcgcg gaggccgtcc tgtcggggat tgaggcccgc
     1021 gcggggacgt cgggcgcggc ggtgccggtc catggcctcg aagacgacgt gggggtggcc
     1081 gatatcgccg gccacacgac gccgctcttc gtgctggggc cgactcgcga ggtgacctgg
     1141 gaagaactca tggggcgtgg gatcgaaccg agcgccctcg gcgtgaccga ccccggagcg
     1201 tcattctgga ccgacgcgtc cacgatgccc ggagtgctcg tcgagctcct gtacgtgacg
     1261 aacccctcgg acgcggcatt gctggcgaac gacgacgctc ggtcggcaat ggcgactggc
     1321 gtggccgacg ggattgcgcg ctacctgggc cagcttgcgg cgaatgaaaa ccggagcgcc
     1381 tcggcggccg gtggatcgtc cgtggccaac agaacggtcg cgagcgcgcc acgtccgcct
     1441 gccggttgcg ccccgcggta gtgacgcggg gcgcccacag gacggcgacc gttgccgtct
     1501 ggcggcacgg agctcaggag gtggacgcct cgatggggtc gaagccgcga cggcgcagct
     1561 cgccttcgag gtcgagggga cgggtgccga accctcgggt ctcccactcc tcgcggggga
     1621 ccatccagaa gacctcgaac tggttgccgt cgggatcctc gccgtacagg ctcaggctgc
     1681 tgccgtggtc ggactggccg gtgagggcgc cggcgttggc gaggtcgacg cgggcgcggg
     1741 cgaggtcggg cagggtgtcg acttcccagg caaggtggta gaggccgacg cgctcacctc
     1801 gggtgggtgg aggtgcggcg gcaccgatgc cgaagaggcc gaggtcgtgg tggttgtcgc
     1861 tgccgttggc gcgcaggaag tgggcgcggt cgcccatcga ttcgacgacg gtgaagccga
     1921 ggacgtcgcg gtagaagtcg agggctgcgt tggcgtcgcg gacccagagg acggcgtgat
     1981 tcaggcggtt gatcgtgaac atggggttct ccttgggtgt cggctgcgac cgggtggagt
     2041 caggggcgcg ggagttgccg aacccccacc cccgccctca cccgcaagcg ggggcggggg
     2101 tgcgcctcga aaggcgatcg gctggctggg cctggcgacc gcgcggatcc gaggttcgaa
     2161 ccgaggcgcg gaatcgccgg atgtggcggt cgatcgcttc ggcatgatcg agcacgactc
     2221 ggcgagcgtc gatgctgtgc gccggctagg gctggtgcac cgcctcgatg gtgagggtga
     2281 tgcggacgcg gtcgccgacg acaaagcctc cggtctcgag gaccgcgttg tagttcagac
     2341 cgaactcctt gcggtcaatc gaggtggtgg cggtgaagcc gatgcggtct tcgccctgga
     2401 agccggtacc gcggccctcg agttcggtgt cgagagtgac cgggcgggtg acgtcgcgga
     2461 tcgtgaggtc gccgtggacg agccagcggt cgtctttgcc gggctcgacg ctggtgctgc
     2521 ggaagcggat ttccgggtag cgctcggcat cgaagaagtt gtcggagcgc aggtcgttgt
     2581 cgcggaactc gacgccggtg ttgacgctcg cggtgcggat gaccgcctcg acggaggagc
     2641 gggtggggtc ggcctcgtcg aggtggatgg agccctcgag gtccgtaaag cggccgcggg
     2701 tgctcgccac catcatgtgc ttcaccgcga actccacgga ggagtgtgcg gcgtcgatgg
     2761 tgtaggtagc ggtctggact gcgggccggg tggtcatctc gtttgctcct tacgtgagtt
     2821 gtggtcactt acggttggta agccgatgat gcagaagaag catacgcttg tcaagcgact
     2881 tatctgtgat aagttgtggg tatgacgacg aagcacgccg aaccgcacgc cgacctcgac
     2941 gcgttctgtc cgcgctacca ccaggcggtg gagctggtcg ggcggcgctg gagcggggcg
     3001 atcctgcgcg cgatgctcgc gggtggtgac cgtttcacgg agctgcggga gacgatcccg
     3061 gggatgagcg accgcttgct ttcggagcgg ttgaaggagt tcgaggcgga ggggctggtg
     3121 gagcggctgg tcttc
//
LOCUS       DAORPG010000171         3134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3009_length_3134_cov_3.599221, whole genome shotgun sequence.
ACCESSION   DAORPG010000171 DAORPG010000000
VERSION     DAORPG010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3009_length_3134_cov_3.599221"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="PLX85_04845"
     CDS             <1..1080
                     /locus_tag="PLX85_04845"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRC62531.1"
                     /translation="PVNIGNPLELSMNEFARRINALTGNAAGIALLMVWLVEVERGSL
                     DLLWIVAMVAVSGVLAALIAVGVFVLFSRTFGIITRVELMELTQLSHPLLRRLQDEAP
                     GTFQHSILVGNLAERAAERIGADPLLVRIGSYYHDIGKLVAPPFFIENSGEESPHQPL
                     DPLQSTRVILQHVTAGIEIARREGLPDAVVQFIPQHHGTRLVTYFYRRAASLDADIDP
                     EMFRYPGPKPQSRETALVMLADSAEATVRASADRSAERIRSIVEAVIRERLEEGQFDE
                     SPISLRDLRVVADVYSSILNSVYHPRVQYPEPTARELASRRGVPLGDTTDSEGPRWPR
                     LPRPRPQLPAAPSPREPVDAAEDTP"
     gene            1129..1827
                     /gene="purQ"
                     /locus_tag="PLX85_04850"
     CDS             1129..1827
                     /gene="purQ"
                     /locus_tag="PLX85_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRC62532.1"
                     /translation="MRAAVLVFPGTWSDGDCVYALSCVGIESRRVWHRDATPFDADEL
                     VVLPGGFSYGDYLRCGAIARFSPVMDSVRRHAEAGGLVIGICNGFQILCEAGLLPGVL
                     MRNDSLQFRCQPTHLVAAEHQSPFTRGIARNRVLEVPISHGEGNYFADEATLDALEAA
                     GRVAFRYCDHAGNVTPEANPNGSLRNIAGVLSEGRNVLGMMPHPERTCDPLLGGEDGN
                     AIWLSILSALPVGA"
     gene            1835..1969
                     /locus_tag="PLX85_04855"
     CDS             1835..1969
                     /locus_tag="PLX85_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015124633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRC62533.1"
                     /translation="MTTTRSYTVVLEPGEDGWIVVRFPALPGCVTQGRTREEALANAR
                     "
     gene            2071..2244
                     /locus_tag="PLX85_04860"
     CDS             2071..2244
                     /locus_tag="PLX85_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011601471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRC62534.1"
                     /translation="MLSGDEVVGVLAKLGYEVRRIRGSHMRLVAAGRPPLSVPRHSEL
                     DQAHSARSFVTQT"
     gene            complement(2310..2783)
                     /locus_tag="PLX85_04865"
     CDS             complement(2310..2783)
                     /locus_tag="PLX85_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRC62535.1"
                     /translation="MTGEALSPEAAWFDSWRGIVFTHARMLDEIGRRLEQHSGISLAF
                     LDVLGRLYDAPDRRLRMQELQRRSLFTRSGMTRVVDRIEEAGLVQRERVPGDRRGVWV
                     VITSKGVRTYEAAMRRHTRDIEEVFGGTMSEAEHRAVGDALWWAWHDEEPVAPPA"
     gene            2926..>3134
                     /locus_tag="PLX85_04870"
     CDS             2926..>3134
                     /locus_tag="PLX85_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase"
                     /protein_id="HRC62536.1"
                     /translation="MTTMFVRHQVQDYAAWRKVYDEFRPTQTRLGVTGQAVYRAADNP
                     NDITVTHEFASLEAAQAFAASAELHG"
BASE COUNT          496 a         1207 c          910 g          521 t
ORIGIN      
        1 ccggtgaaca tcggcaaccc gctcgagctg tcgatgaacg agttcgcgcg gcggatcaac
       61 gccctcaccg gcaacgccgc cggcatcgcc ctcctcatgg tctggctcgt cgaggtcgaa
      121 cgaggcagcc tcgacctgct ctggatcgtg gccatggtcg ccgtcagcgg cgtccttgcc
      181 gccctgatcg cggtcggcgt cttcgtcctc ttcagccgga cgttcggcat catcactcgc
      241 gtcgagctga tggagctcac gcagctcagc caccccttgc tgcgccgcct gcaggacgag
      301 gctcccggca ctttccagca ctcgatcctc gtcggcaacc tcgccgagcg tgcggctgag
      361 cgcatcggcg ctgacccgct cctcgtccgc atcggctcct actaccacga catcgggaaa
      421 ctcgtcgcgc cccccttctt catcgagaac agcggcgagg agtccccgca ccagccgctc
      481 gacccgctgc agagcacccg cgtcatcctc cagcacgtca ctgccggcat cgagatcgcc
      541 cgtcgcgagg gcctccctga cgcggtggtg cagttcatcc cccagcacca cggcacccgg
      601 ctcgtcacct acttctaccg ccgcgccgcc tcgcttgacg ccgatatcga tcccgagatg
      661 tttcgctacc ccggcccgaa gccgcagtcg cgcgagacgg ccctcgtaat gctcgccgac
      721 tccgccgagg ccacagtccg ggcttcggca gaccgctccg ccgagcgcat ccgcagcatc
      781 gtcgaggccg tcatccggga gcgcctcgag gaaggccagt tcgacgagag cccgatctca
      841 ctgcgcgacc tgcgtgtcgt cgccgatgtc tactcgtcca tcctcaactc ggtctaccac
      901 ccacgcgtcc agtaccccga gcccaccgcg cgcgaactgg ccagccgccg cggcgttcct
      961 ctcggcgata ccacagactc cgagggcccg cgctggcctc gccttccgcg tccccgcccc
     1021 cagcttcccg ccgccccgtc gccgcgcgaa ccagtcgacg ccgccgagga cactccctag
     1081 acaggtcgcc ttctatactg ggaacccccc acgatcgcgg aggtttcgat gcgcgccgcc
     1141 gtcctcgtct ttcccggcac ctggagcgat ggcgactgcg tctacgccct cagctgcgtc
     1201 ggcatcgagt cgcgccgcgt ctggcaccgc gacgccactc ctttcgacgc ggacgagctc
     1261 gtcgtccttc caggcggctt ctcctatggc gactacctgc gctgcggcgc gatcgcccgc
     1321 ttctcgcccg tgatggactc cgtccgtcgc cacgccgagg ccgggggcct cgtgatcggc
     1381 atctgcaacg gcttccagat cctctgcgaa gcaggactgc ttccaggtgt cctcatgcgc
     1441 aacgactcac tgcagttccg ctgccagccc acacacctcg tcgccgccga gcaccagtcg
     1501 ccgttcacgc gcggcatcgc ccgcaaccgc gtcctcgagg ttccgatctc ccacggcgaa
     1561 ggcaactatt tcgctgacga ggcgacgctc gatgctctcg aggcggccgg gcgcgtcgcg
     1621 ttccgctact gcgaccacgc cggcaacgtc acccccgagg ccaacccgaa cggctcgctg
     1681 cgcaacatcg ccggtgtgct gtctgagggc cgcaacgtcc tcggcatgat gccccacccc
     1741 gaacgcacgt gtgaccccct cctcggcggc gaggacggca acgccatctg gctctccatc
     1801 ctcagcgccc tccccgtcgg cgcataatgg gggcatgacc accactcgca gctacaccgt
     1861 cgtcctcgaa cccggcgaag atggctggat cgtcgttcgc ttcccggccc tgcctggttg
     1921 tgtgactcag ggccgtacgc gcgaggaagc cctcgctaac gcgcgctagg ttctcctact
     1981 gaccctcgag tcgatgttgg aagaggggac ctcgttgcca gacgaggact cgctgacact
     2041 tgaggtcgcg gtgtagggcc ggcgttgccc gtgctgtccg gcgatgaagt cgtcggcgtc
     2101 ctcgcaaagc tgggctacga ggtcaggcgc atccgaggca gccacatgcg actggtcgcc
     2161 gctggtcggc ctccgctctc cgtcccgcgg cattccgaac tcgaccaggc acactccgca
     2221 cgatcattcg tgacgcagac ctgacggtcg aggagttcgt cgccctgctg gaaagctgag
     2281 acttcgcccg ccgatctgac gccagccggc tacgccgggg gcgccaccgg ctcctcgtcg
     2341 tgccacgccc accacagcgc gtcgccgacc gcgcgatgct ccgcctcgct catcgtcccg
     2401 ccgaacacct cttcgatgtc ccgtgtgtgt cgtcgcatcg cggcctcgta cgtccgcacg
     2461 cccttcgacg taatgaccac ccacacccct cggcggtcgc ccggcacccg ctcgcgttgc
     2521 accaggcccg cttcctcgat ccggtccacg acccgagtca tcccgctgcg cgtgaacagc
     2581 gatcggcgct gcagctcctg catccgcagc cgtcggtcgg gcgcgtcgta gagccgtccg
     2641 agcacgtcga ggaacgcgag cgagatcccc gagtgctgct ccagtcggcg cccgatctcg
     2701 tccagcatcc gtgcgtgggt gaacacgatc cctcgccacg aatcaaacca cgctgcctcc
     2761 ggggacagag cttcaccggt catagtccta ggctatcggg agaaatgaca cagtgcaatt
     2821 tcactcacta cagtagttga caagacagat actttcatag caatatgccc gagcgcatcg
     2881 attacggaca gatgcgccgg tcagcccagc agaaggaggc gaaacgtgac gaccatgttt
     2941 gtgcgacacc aggtgcagga ctacgccgcg tggcggaagg tctacgacga gtttcggccc
     3001 acccagacca ggctcggggt gaccggccag gccgtgtacc gcgctgccga caacccgaac
     3061 gacatcaccg tgacgcacga gttcgcctcc ctcgaggcgg cccaggcctt cgccgcgagc
     3121 gccgagctgc acgg
//
LOCUS       DAORPG010000172         3122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3033_length_3122_cov_2.957939, whole genome shotgun sequence.
ACCESSION   DAORPG010000172 DAORPG010000000
VERSION     DAORPG010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3033_length_3122_cov_2.957939"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..741)
                     /locus_tag="PLX85_04875"
     CDS             complement(10..741)
                     /locus_tag="PLX85_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HRC62537.1"
                     /translation="MDMHVHTMGASSDSMLKPEDLPEIGRANGLTGFNIAEHDQVWER
                     YRRDAYHEQHQDIFANFGMEVSTDHGHILAIGLEGYISGIRRLAQLREEIDKVGGFLI
                     VAHPFRHVFDPVTAMRKGGRPFDLSPEQAADLPVFKLVDAIEVANGCNTPRENEFAYQ
                     VAKILGKSGTGGSDAHSNTGIGFFATGFHRVITTQTQLLEELHAGRFEAVHRTPAGRL
                     VKFEVGSIDAAQNDRAPASEPSVTA"
     gene            complement(881..1300)
                     /locus_tag="PLX85_04880"
     CDS             complement(881..1300)
                     /locus_tag="PLX85_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62538.1"
                     /translation="MSDETARALLSGFISGAAAALVSTFVMLVLLSRSSVWQRLPENR
                     RVPMPLVGVVFVNLMMLLWTAAGLILGALYLHFASGRAEDNALVPHLEFSLVVTAVIG
                     IALATAAVIRGRLGTIAPLLALIAHLAFAWMLPNLAR"
     gene            complement(1297..2193)
                     /gene="ispE"
                     /locus_tag="PLX85_04885"
     CDS             complement(1297..2193)
                     /gene="ispE"
                     /locus_tag="PLX85_04885"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRC62539.1"
                     /translation="MSALRLRAPAKINLALEVTGRRPDGYHDIDTVMTTIDLADDLRL
                     SPARALEVVLSGAESKDIDPVDDLSGRAARALAVAAGREPAVRIEVTKRIPSPAGLGG
                     GSSDAAAVLRGLNVLWDLDWPLTRLSEVAATVGSDVPFFLHGGAARCTGRGEVVEPLR
                     DLRPLRLLILVPPVGAMANKTARRYGALTPHDFTDGHRSWRLSQRLARGAPPPTNDLV
                     NVFETVVERTDAELLAHYASYRAAGAPQLHLCGAGPAVYVFVHEGARVADLRRGFEAL
                     GARVLEARTLPRAAAQAIAPEP"
     gene            complement(2203..>3122)
                     /gene="rsmA"
                     /locus_tag="PLX85_04890"
     CDS             complement(2203..>3122)
                     /gene="rsmA"
                     /locus_tag="PLX85_04890"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRC62540.1"
                     /translation="LPSRHGQSVTRRAGRVQRPHFVSPPEALRRLGVTPRKALGQHFL
                     LDQFILGDIAEACLLTSEDTVLEIGAGPGGLTEELAQRAGHVVAVELDEELAALSRRR
                     LADRANVSIVAADVLDYTPAELLAECEAAPPYIATGNLPYYITQPVVRRLLEADPSPE
                     RIVVLVQREVARRMVGGPGHESLLSMSIKCYGTPEPLFDVPPSAFWPPPKVQSAAVRI
                     ERLPAPVLSRELLPRFFNLLRAGFSEPRKQLHNAIRNSFGIPDAAARELLEEAGIDVM
                     ARAQHLDLTDWQRLFAVVERRHPRALNLD"
BASE COUNT          512 a          952 c         1227 g          431 t
ORIGIN      
        1 cgggcgggat caggcggtga cggacggctc cgaagcaggg gcgcggtcgt tctgagccgc
       61 gtcgatgctg ccgacctcga acttcacgag gcggccagcg ggcgtacgat gcaccgcctc
      121 gaagcgcccg gcgtggagct cctcgagcaa ctgtgtctgc gtggtgatca cgcggtggaa
      181 cccggtagcg aagaagccga tgcccgtgtt ggagtgcgcg tcgctgccgc ccgtgccgga
      241 cttgccgagg atcttcgcga cctggtaggc gaactcgttc tcgcgcggcg tgttgcaacc
      301 gttggccacc tcgatggcat cgacgagctt gaagacgggc aggtcggcgg cctgctctgg
      361 actgaggtca aaggggcggc cgcccttgcg catcgcggtg acggggtcga agacgtggcg
      421 gaaggggtgg gcgacgatca ggaagccgcc gaccttatcg atctcctcgc ggagctgggc
      481 gaggcggcga atgccgctga tgtagccctc gagaccgatg gcgaggatgt gaccgtgatc
      541 ggtggacacc tccatgccga agttggcgaa gatgtcctgg tgctgctcgt ggtaagcgtc
      601 gcgccggtag cgctcccaga cctggtcatg ttcggcgatg ttgaagccgg tcagcccgtt
      661 cgcgcggccg atctcgggga ggtcttcggg tttgagcatg gagtcggacg aggcgcccat
      721 cgtgtggaca tgcatgtcca tgatggtcgc gctcagaccg tcattcaggg taggttccag
      781 cggtagcctc cctcgttcgt ctcctgagca tacgtgacag cgaaagggaa gtgccaccct
      841 gccccccgcg tcccctcgaa acggcctctc gcgcgggcga tcagcgggcg aggttgggca
      901 gcatccaggc gaaggcgagg tgggcgatca gcgccaggag cggcgcaatc gtgccgagcc
      961 gtcctcggat caccgcggcg gtcgccaggg cgatgccgat gacggcggtg acgacaagcg
     1021 agaattcgag gtgggggacc agcgcgttgt cctcggctcg cccggacgcg aagtgcaggt
     1081 agagcgcgcc gaggatgagg ccggccgcag tccagagcag catcatcagg ttgacgaaca
     1141 cgacgcccac gaggggcatc gggacacggc ggttctccgg cagccgttgc cacacggagc
     1201 tgcgagagag cagcaccagc atgacaaagg tgctgaccag cgccgcagcg gcgcccgaga
     1261 tgaagccgga gaggagcgcg cgagcggtct cgtcgctcac ggttcggggg cgatcgcctg
     1321 cgccgcggcg cgaggcagcg tacgcgcctc aagaacgcgt gcgccgagcg cctcgaagcc
     1381 ccgcctgagg tccgcgacac gagcgccctc gtggacgaag acgtagacgg ccggccccgc
     1441 accacagagg tggagctgcg gcgcgcccgc ggcgcggtac gaggcatagt gcgcgagcag
     1501 ctcggcgtcg gtgcgctcga ccaccgtctc gaagacgttg acgaggtcat tcgtgggcgg
     1561 cggcgcgcct cgtgcgaggc gttgcgagag ccgccatgag cgatgtccgt cggtgaagtc
     1621 gtgcggagtg agcgcgccgt aacggcgagc ggtcttgttc gccatcgcgc cgacgggcgg
     1681 cacgaggatg aggaggcgca gcgggcggag atcgcgcagc ggctcgacga cctccccgcg
     1741 gccggtgcaa cgggcagcgc cgccatgcag gaagaacggc acgtcggagc cgaccgtggc
     1801 cgccacctcg gagaggcgtg tcagcggcca gtcgaggtcc cagagtacgt tgagccctcg
     1861 gaggacggcc gcggcgtctg atgagccgcc gcccaggccc gcgggcgagg ggatgcgctt
     1921 ggtgacctcg atgcggacgg cgggctcgcg gccggcggcg acggcaaggg cgcgggcggc
     1981 ccgacccgag aggtcgtcga cagggtcgat gtctttcgac tcggcgcccg agagtacgac
     2041 ctcgagcgcg cgggcgggcg acaggcggag gtcgtcggcg aggtcgatgg tggtcatgac
     2101 ggtgtcgatg tcgtggtagc cgtcgggacg gcggccggtc acctcgagcg caaggttgat
     2161 cttcgcggga gcgcggaggc ggagggcaga cacctcgaca gcctagtcga ggttcagggc
     2221 gcgcgggtgg cggcgctcga cgacggcgaa cagccgctgc cagtcggtga gatcaaggtg
     2281 ctgggcgcgc gccatgacat cgatgccggc ctcctcgagc agctcgcgtg cggcggcatc
     2341 tgggatgccg aacgaattgc ggatcgcgtt gtggagctgc ttgcgaggct cggagaagcc
     2401 cgcccggagc aggttgaaga acctcggcag gagttcccgg gagaggacgg gggcgggcag
     2461 gcgttcgatg cgcaccgccg cggactgcac cttcggcggc ggccagaagg cggagggagg
     2521 cacgtcgaag agcggctcag gggtgccgta gcacttgatg gacatggaga ggagcgactc
     2581 gtggccgggc ccgccgacca tgcggcgtgc gacctcgcgc tggaccagca caacgatgcg
     2641 ctcgggggac gggtcggcct cgaggaggcg gcggacgacc ggctgcgtga tgtagtacgg
     2701 aaggttgccg gtagcgatat acggcggagc cgcctcgcac tccgcgagca gctcggcggg
     2761 agtgtagtcg aggacatcgg ccgccacgat ggagacgttc gcgcggtcgg caaggcgtcg
     2821 gcgggagagc gcggccaatt cctcgtcgag ctcgacggca acgacgtggc ccgcgcgctg
     2881 ggcgagctcc tcggtaaggc cacccggacc ggcgccgatc tcgagcacgg tgtcctcgga
     2941 cgtcagcagg caggcctcag cgatgtctcc gaggatgaac tggtcgagga ggaagtgctg
     3001 accgagcgcc ttcctcggcg tgacgccgag gcgacgcagc gcctccgggg gcgagacgaa
     3061 gtggggccgc tgaacacggc cggctcgccg ggtcacggac tggccgtgac gcgaggggag
     3121 gg
//
LOCUS       DAORPG010000173         3115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3051_length_3115_cov_3.465033, whole genome shotgun sequence.
ACCESSION   DAORPG010000173 DAORPG010000000
VERSION     DAORPG010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3051_length_3115_cov_3.465033"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1532)
                     /locus_tag="PLX85_04895"
     CDS             complement(<1..1532)
                     /locus_tag="PLX85_04895"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRC62541.1"
                     /translation="MPKYIFVTGGVVSSVGKGIVTASLGRILKSRGHRVSILKLDPYI
                     NVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFLDDNLTRANSTSTGQVYEEVIRME
                     RRGDFLGGTIQPIPHITNEIKRRIRSAQQALDADIVLVEVGGTVGDMEGQPFLEAISQ
                     MRHALEPHDTMSIHVTLLPYIGATKELKTKPTQHSVRELRTFGIQPDVIVARSDFPVG
                     QSLREKISLFCNVDTEAVIALETADSIYEVPLYLEAAGLSQIVARQLELPVAEADLED
                     WRGFVQRLREPSATARIALVGKYVELHDAYLSIKEALIHAGVHHGAKLDLRWVHSEDL
                     ESRPPEEVIGDVDGILLCPGFGDRGLEGKIAAVRYAREGLIPYLGDCLGLQMMVCEFA
                     RNVAGMPGANTTEADPRTPFPVISLLSEQRGVDELGGTMRLGGYDCRLVEGTRAAEAY
                     GASNVRERHRHRYEVNNALLPVLQEHGLVASGWSPDGRLVEICELRDHPWMVGAQFHP
                     EFT"
     gene            1728..3002
                     /locus_tag="PLX85_04900"
     CDS             1728..3002
                     /locus_tag="PLX85_04900"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRC62542.1"
                     /translation="MAAIPELTTLPNGLRVLTTPLPTAQAASIAFFAGIGSRGESART
                     NGLSHYLEHMLFKGTEQRPNAQLISQAIEGAGGTLNAYTTHELTCYWNNVPFEAAATG
                     ISVVADMIQRSLLLQEEIDRERTVVQQEIRRGHDTPASWVGELLSRATFGDQPIGWPV
                     AGTIDTVEGMHRADFTEHMQRFYTSTNAVFSVAGNVTHEQVVRLAEQHFGDLPTSTPD
                     RSPAANWGLPPERVIVEERETEQTQVALSMQAIARRDPDRYALEILHTILGRGMSSRL
                     FEEVRERRGLAYSVGSRITRYQDIGSFTVAAGVTRAQQEEALQVIVEQLHRIAEEPVD
                     AEELRRAIDYAAGSFRLSFETPMSYGQRFGEQLLQDGEIEPLDETVARLRAVTAEDVQ
                     RVGQRVIGRREYSLAVVGPSASADRLAEILAA"
BASE COUNT          501 a         1145 c          969 g          500 t
ORIGIN      
        1 gtgaactccg ggtggaactg cgccccgacc atccagggat ggtcgcgcaa ctcacagatc
       61 tcgaccagcc gcccatccgg cgaccatccg gaggcgacga gcccgtgctc ctgcagcacc
      121 ggcagcagcg cgttgttgac ctcatagcgg tggcgatgcc gttcccgcac gttcgacgcg
      181 ccgtacgcct cggccgcacg cgtcccctcg acgagccggc agtcgtaccc gccgaggcgc
      241 atcgtgccgc ccagctcgtc gacgccgcgc tgctccgaca gcagcgagat caccgggaat
      301 ggtgtccgcg ggtccgcctc ggtcgtattc gcgcccggca tcccggcaac gttgcgcgcg
      361 aactcgcaca ccatcatctg cagccccagg cagtcaccga ggtatgggat gaggccctcg
      421 cgtgcgtaac gcaccgccgc gatcttcccc tcgaggccgc ggtcgccaaa gccggggcag
      481 agcagaatgc catcgacgtc gccgatcact tcctcgggcg ggcgcgactc caggtcttcc
      541 gaatgcaccc agcgcaaatc gagcttcgct ccgtggtgca cgccggcgtg gatgagcgcc
      601 tccttgatcg acaggtaggc gtcatgcagt tccacgtact tcccgacgag ggcgatacgc
      661 gccgtcgccg agggctcgcg cagccgctgc acaaagcccc gccagtcctc gaggtctgcc
      721 tccgccaccg gcagctccag ctgcctggcc acgatctgcg acaacccggc cgcctcgaga
      781 tacagcggta cctcgtagat cgagtcggca gtctcgaggg cgatcacggc ctcggtgtcc
      841 acgttgcaga acaacgagat cttctcgcgc agcgactgcc caacaggaaa gtcggaccgc
      901 gccacaatca cgtccggctg aatgccgaac gtccgcagtt cacgcaccga gtgctgcgtg
      961 ggcttggtct tcaactcttt cgtcgccccg atgtacggca gcagcgtcac gtggatcgac
     1021 atcgtgtcgt gcggctcgag ggcgtgccgc atctggctga tcgcctcgag gaacggctgc
     1081 ccctccatgt cgccgaccgt gccgcccact tcgacgagca cgatgtccgc gtccagcgcc
     1141 tgctgcgccg accggatgcg tcgcttgatc tcgttggtga tgtgggggat cggctggatg
     1201 gtgccgccga ggaagtctcc tcgccgttcc atccggatca cttcctcgta cacctgtcct
     1261 gtcgaggtcg agttcgctcg cgtcaggttg tcatcgagga agcgctcgta gtgccccagg
     1321 tcgaggtcgg tctcggcgcc gtcatcggtg acgtacacct cgccgtgctg gtacggcgac
     1381 atcgtcccgg ggtcgacgtt gatgtacggg tccagcttga ggatggacac gcggtgtccg
     1441 cgcgacttga ggatgcgtcc gagcgaggcg gtcacaatcc ccttccccac ggaactcacg
     1501 actccgccgg tgacgaagat gtacttcggc atgtcaggtg cctcgggtgc ggggggaggt
     1561 gaagggggac acctccaatc ctccgtaacc cctcggcggg tgtcaaccga aacccgacgc
     1621 tccgctcctc cttgcgaggc ctatgggcgc aacgtaggct gagggcacac gctccgcggc
     1681 gcccacggcg ctgtaccctc gtcccgcggt ccacgagagg cgacgcgatg gcggccatcc
     1741 ccgaactcac gacgcttccc aacggcctgc gcgtcctcac cacaccgctg ccgaccgcgc
     1801 aggcggcgag catcgccttc ttcgccggca tcggctcgcg aggcgagtcc gcccgcacca
     1861 acggcctctc gcactacctc gagcacatgc tcttcaaggg caccgaacag cgcccgaacg
     1921 cgcagctcat cagccaggcg atcgagggcg ccggcggcac cctcaacgcc tacacgacgc
     1981 acgaactgac ctgctattgg aacaacgtcc ccttcgaggc cgcagccacc ggcatctccg
     2041 tcgtcgccga tatgatccag cgctccctgc tcctccagga ggagatcgac cgcgagcgta
     2101 ccgtggtgca gcaggagatc cgccggggtc acgacacccc cgcctcgtgg gtgggcgagc
     2161 tgctcagccg cgccaccttc ggcgaccagc cgatcggctg gcccgtcgcg ggcaccatcg
     2221 acaccgtcga gggcatgcat cgcgccgact tcaccgagca catgcagcgc ttctacacct
     2281 cgacgaacgc ggtcttctca gtcgccggca acgtcaccca cgagcaggtc gtccggcttg
     2341 ccgagcagca cttcggcgac ctcccgacct cgaccccgga ccgctcgccc gccgcgaact
     2401 ggggcctccc cccggaacgc gtgatcgtcg aggagcgcga gaccgagcag acgcaggtcg
     2461 ccctgtcgat gcaggccatc gcccgccgcg accccgaccg ctacgccctc gagatcctgc
     2521 acacgatcct cgggcgcggg atgtcctccc gcctcttcga ggaggtgcgc gagcgtcggg
     2581 gcctcgcgta ctcagtcggc tcacgcatca cgcgctacca ggacatcggc tcattcacgg
     2641 ttgcggccgg cgtgacccgg gcccagcagg aggaggcgct gcaggtcatc gtcgagcagc
     2701 tacatcgcat cgctgaggag cccgtcgatg ccgaggaact gcggcgcgcc atcgactacg
     2761 ccgcgggctc gttccgcctc tcctttgaga cgccgatgtc gtatggccaa cgcttcggtg
     2821 agcagctgct ccaggacggc gagatcgaac ccctcgatga gacggtcgcc cgtctgcgcg
     2881 ccgtcaccgc cgaagacgtg cagcgtgtcg gccagcgcgt catcggtcgc cgtgagtact
     2941 cgcttgcggt cgttgggccc tcggccagcg ccgaccgcct cgccgagatc ttggccgcct
     3001 gagctctcga tgccgttcat cacgaccccc gacggcgtcc agatccgcta tcaggagttc
     3061 ggcagcgggc gaccgctcgt cctcgcgcac ggcttcagcg catcgctgga catgt
//
LOCUS       DAORPG010000174         3082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3104_length_3082_cov_3.796168, whole genome shotgun sequence.
ACCESSION   DAORPG010000174 DAORPG010000000
VERSION     DAORPG010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3104_length_3082_cov_3.796168"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="PLX85_04905"
     CDS             complement(<1..1077)
                     /locus_tag="PLX85_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008387626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRC62543.1"
                     /translation="MTTAAPKDSPAVANKQVYLFGDGAAEADGMMKELLGGKGAGLAE
                     MSRLGIPVPPGFTITTEVCNTFYALGRQYPDGLEARVREGIAHIERAVGAGFGDATNP
                     LLVSVRSGARVSMPGMMDTVLNLGLNDVTAEGLAERSGSGRFAYDSYRRFVQMYGDVV
                     LGVKPDSDTEHDPFEVLLDAKKAARGVKLDTELTEADLRELVVEYKGAIRDRLGIEFP
                     DDPWQQLWGAIGAVFNSWENPRAIAYRSMYGYPSNWGTAVNVQAMVFGNLGEDCATGV
                     AFTRHPATGDRHLYGEFLLNAQGEDVVAGIRTPQPIGKVDRVPGGPASLEETMPVVHG
                     ELVEACDRLEAHFRDMQDIEFTVQQ"
     gene            1298..2329
                     /locus_tag="PLX85_04910"
     CDS             1298..2329
                     /locus_tag="PLX85_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRC62544.1"
                     /translation="MLASVRALLEYREASLASAAARESASRGRDVPEPPDPLRLEYQV
                     DRDRIVHTRAFRRLKHKTQVFVVADSDHVVTRMTHTIEVQQVARTIARALALNEDLTE
                     AIAWGHDLGHTPFGHAGEEALGRLLPGGFRHNEQSLRIVEQLARDGRGLNLTVEVREG
                     ILKHSKTRESIAAEAWGTADTLEGQIVKLADSIAYLNHDIEDAVQAHLIEEHDLPRAV
                     HTVLGATHDDRIKTLVADCVEQSWEAAQGRAEKRITLSAPVLEATDELREFMFQRVYL
                     VEPTLREAQRGQRVVEALFRYYEARPEEIAGWSLSSDPAWRRAADYVSGMTDGFAVRR
                     AEALGLAFE"
     gene            2384..2818
                     /locus_tag="PLX85_04915"
     CDS             2384..2818
                     /locus_tag="PLX85_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62545.1"
                     /translation="MDADDLRQQVSAARARLIASLEGASESDFNADLEPGVTVVGALA
                     DLARRQRAALEDARRLAELAPRPSPVAHGDATRRLTPPPVVHDLAGAHHEAILLIDAL
                     AALDPAQTGVARAAGDTLLAVIGEEHALADRIALRIGRTPAA"
     gene            2849..>3082
                     /locus_tag="PLX85_04920"
     CDS             2849..>3082
                     /locus_tag="PLX85_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HRC62546.1"
                     /translation="MTTIDQVKDQLDLVEVVRGYVPALKKSGRTWKSPCPFHSERTPS
                     FVVDPDRGTWHCFGACATGGGVIEFVRRIEHLDF"
BASE COUNT          494 a         1144 c          978 g          466 t
ORIGIN      
        1 ctgctgcacg gtgaactcga tgtcctgcat gtccctgaag tgcgcctcga ggcgatcgca
       61 tgcctcgacc agctccccgt gcacgaccgg catcgtctcc tcgagggacg cgggcccgcc
      121 agggacgcgg tcgaccttgc cgatcggctg cggcgtacgg atgccggcga cgacgtcctc
      181 gccctgcgca ttcagcagga actcgccgta caggtggcgg tcccccgtcg ccgggtgccg
      241 cgtgaacgcg acgcccgtcg cgcagtcctc gccgaggttg ccgaagacca tcgcctgcac
      301 gttgaccgcc gtgccccagt tcgagggata gccgtacatc gagcggtacg cgatcgcgcg
      361 cggattctcc cacgagttga acaccgcgcc gatcgcgccc cacagctgct gccaggggtc
      421 gtccgggaac tcgatgccga ggcggtcgcg aatcgcgccc ttgtactcga cgaccagctc
      481 ccgcaggtcg gcctcggtca gttcggtatc gagcttgacg ccgcgcgccg ccttcttcgc
      541 gtcgaggagc acctcaaacg ggtcgtgctc ggtgtcggag tccggcttca cgccgaggac
      601 gacatccccg tacatctgca caaaccggcg gtacgagtcg taagcgaacc gcccgctgcc
      661 ggagcgctca gccaacccct cggccgtcac gtcgttcagc ccgaggttga ggacggtgtc
      721 catcatgccc ggcatcgaca cccgcgcacc ggagcgcacc gacacaagca ggggattcgt
      781 cgcatccccg aaacccgcgc cgaccgcgcg ctcgatgtgc gcgatgccct cgcgtacccg
      841 cgcttccagg ccatccgggt actggcggcc gagcgcatag aaggtgttgc agacttcagt
      901 ggtgatcgtg aatcccggcg gcaccgggat cccgaggcgg ctcatctccg ccagcccggc
      961 gcccttgccg ccgagcagct ccttcatcat cccgtcggcc tcggcggcgc catcgccgaa
     1021 cagatacacc tgtttgttcg ctacggcggg tgaatccttc ggcgcggctg tcgtcatcga
     1081 gcacctctca atgcccgcgc ctctcgcgat gcacgactcg ctacgctcag ttcctccggc
     1141 gggcgcagac cggactgaag cgcacgaggt agctcaggga ggcgagctgt ctgtgggtcg
     1201 attataagga gcagacgaac cagcagaaag cgaacgcgcg ggctgcggag tctgctgccg
     1261 ccttgtccgg cgcatctccg acgcccacac tcgacccatg ctggcgtcgg tccgcgcact
     1321 gctcgaatat cgcgaagcgt cactcgcatc cgccgccgcg cgcgagtctg cctcgcgcgg
     1381 ccgcgacgtc cctgagccgc ccgacccgct ccgcctcgag taccaggtcg accgcgaccg
     1441 tatcgtgcac acgcgcgcat ttcgccgcct caagcacaag acgcaggtct ttgtcgtggc
     1501 cgactcggac cacgtagtga cccgcatgac ccacaccatc gaagtgcaac aggtcgcgag
     1561 gaccatcgcg cgcgcgctcg cactgaacga ggatctgacc gaagccatcg cctggggcca
     1621 cgatctcggc cacactccgt tcggccatgc cggcgaggaa gcgctcggcc gcctcctgcc
     1681 gggcgggttc cgccacaacg aacagtcgct ccgcatcgtc gagcagctcg cccgcgacgg
     1741 ccgcggcctc aacctcaccg tcgaggtgcg ggagggcatc ctgaagcact ccaagacccg
     1801 cgaatcgatc gcggccgagg cctggggcac ggccgacacc ctcgagggcc agatcgtgaa
     1861 gctcgccgac tcgatcgcgt acctcaacca cgacatcgag gacgccgtgc aggcgcacct
     1921 gatcgaggag cacgatctcc ctcgggcggt tcacaccgtg ctcggtgcca cccacgacga
     1981 tcgcatcaag acgctggtcg ccgactgcgt tgagcagtcg tgggaggccg cgcagggccg
     2041 cgccgagaag cgcatcacac tgagcgcgcc agtcctcgag gcgaccgatg aattgcgcga
     2101 gttcatgttc cagcgggtct acctcgtcga gcccacactg cgggaggccc agcgcggcca
     2161 gcgggtcgtc gaggcgctat tccgctacta cgaagcgcgt cccgaagaga tcgccgggtg
     2221 gtcgctgtcc tcagacccgg cctggcggcg cgccgctgac tacgtctcgg gcatgacgga
     2281 cggctttgcc gtccgccgtg ccgaggcact cgggctcgcg ttcgagtagc ggccgcgctc
     2341 cgctcgcttt cggggcggcg cgtcgaggac aatagccgcg ccgatggacg ccgacgatct
     2401 ccgccagcag gtcagcgcag cacgcgctcg cctgatcgcg tctctcgaag gcgcgtcaga
     2461 gagcgacttc aacgcagacc tcgaacccgg ggtgacggtc gtcggcgcgc tcgcggacct
     2521 cgcccggcgg caacgtgccg ctctcgagga cgcccgacgc ctcgccgaac tcgcgccccg
     2581 gccttcacct gtggcgcatg gcgacgccac ccggcgcctc acgccacccc cggtcgtcca
     2641 cgacctcgcc ggagcccatc acgaggcaat cctcctcatc gacgcgctcg cggcactgga
     2701 cccagctcag accggcgtcg ctcgggccgc cggcgacaca ctcctcgcgg tcatcggcga
     2761 ggaacatgcg ctggccgacc ggattgcgct ccgcatcggc cgaacgcccg ccgcgtaacg
     2821 atccgcgggc tgcggtgtct tatgaagtgt gaccaccatc gaccaggtga aagaccagct
     2881 cgacctcgtg gaggtcgtgc gcggttacgt gcccgcgctg aagaagagtg ggcgcacctg
     2941 gaagtcgccc tgcccctttc acagcgagcg cacgccgtcg ttcgtcgtcg accctgaccg
     3001 cggcacctgg cactgcttcg gcgcctgcgc cacgggcggc ggcgtcatcg agttcgtccg
     3061 ccgcatcgag cacctcgact tc
//
LOCUS       DAORPG010000175         3077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3116_length_3077_cov_3.310721, whole genome shotgun sequence.
ACCESSION   DAORPG010000175 DAORPG010000000
VERSION     DAORPG010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3116_length_3077_cov_3.310721"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..1587
                     /gene="ahcY"
                     /locus_tag="PLX85_04925"
     CDS             301..1587
                     /gene="ahcY"
                     /locus_tag="PLX85_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRC62547.1"
                     /translation="MTTVQNAPGAAGHIADPGLALQGVRRIEWAAREMPVLAQIRERF
                     AKERPFEGVRMAACLHITSETANLALALHDGGADLRLAASNPLSTQDEVAAALVAEYG
                     IPTFAIKGEDSATYYQHLNSAADHGPQLTMDDGADMVALLHRERRDLLAGVIGGTEET
                     TTGVVRLRALAAEGKLGYPIVAVNEADTKHMFDNRYGTGQSTIDGITRATNILLAGKN
                     LVVCGYGWCGKGVASRARGLGAQVIVTEVQPLRALEAVMDGFRVMPILDAALIGDIFV
                     TVTGDINVIDAPSFEAMKTGAVLANSGHFDAEINLKALEGMTEARRPLRPFVEEFTLQ
                     DGRKLVVLAEGRLINLGAAEGHPASVMDMSFANQALCSEYLVAQHASLKPDVYNVPRD
                     IDDEVARLKLAAMGVAIDTLTEEQHRYLNSWELGTQ"
     gene            1666..2538
                     /gene="mtnP"
                     /locus_tag="PLX85_04930"
     CDS             1666..2538
                     /gene="mtnP"
                     /locus_tag="PLX85_04930"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HRC62548.1"
                     /translation="MASTADIAVIGGSGFYDLEGLRDARPLEVDTPFGATSSPILVGE
                     LHGKRVAFIARHGEGHRILPAEVPSRANLWALKHLGVRRVLAVSAVGSLQQQYHPLDA
                     VVPDQLLDRTRGRASTYFGDGVVAHIGFADPFCDETREALAAASEAAGVTTHRGGTLV
                     VIEGPAFSTRAESRLYQSWGAAVIGMTALPEAKLAREAELCYASICYVTDYDVWHDQE
                     EDVGVELVVENMRRNIQHARDTVSRTIAALPIEGACACGDALNGAVITAPDAVPPATR
                     QRLAFMLDRYWGAQ"
     gene            2535..>3077
                     /locus_tag="PLX85_04935"
     CDS             2535..>3077
                     /locus_tag="PLX85_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62549.1"
                     /translation="MTDALYGPAAEARPRFRVRRVVDRRVLDAALSEDRAYAAYALGH
                     LEPGLFEQSRFWLAEGPSGSGVLLHADGLLGRTTVVVGDPHAVDALVALHPGTRGSYL
                     ATGSPDHLEALRRTYLVLGSLEMRRMSVNAATFAPRPSLRHHVRRLGGHDTRALNMLY
                     STEGPPTAYTADQVNQSVYYG"
BASE COUNT          481 a         1296 c          862 g          438 t
ORIGIN      
        1 ccgtcgccgc cttcggtgat gcgcgacctc cacccgagca ccgccaccac cggcagccga
       61 tcccgatctt tcccgtcccc tcgcgggggg aaggcgcggg aggggtcccc acacccacca
      121 tggcgaaccc ggactccaag cccgccaggg ggctccgaag accagcgact ccatcgaggc
      181 gcccgcacac tggcggccac ccgcccccgc cccgaaaata gaacccctca gctaccatcc
      241 ctacatacgc ccccgcccct ccatcgaggg ccggccagcg tgcgctccga ggaggaacac
      301 atgacgacgg tgcagaacgc ccccggcgcc gccggccaca tcgccgaccc cggcctcgcc
      361 ctccagggcg ttcgccgcat tgaatgggcc gcgcgcgaaa tgcccgtcct cgcccagatc
      421 cgtgagcgct tcgcaaaaga acgccccttc gagggcgtcc gcatggccgc ctgcctccac
      481 atcacctcgg aaactgccaa cctcgccctc gccctccacg acggcggcgc tgacctccgc
      541 ctcgccgcct caaacccgct ctctacccag gacgaagtag ccgccgccct ggtcgccgag
      601 tacggcatcc ccactttcgc catcaagggc gaagacagcg ccacctacta ccagcacctg
      661 aacagcgctg ccgaccacgg cccgcagctc acgatggacg acggcgcaga catggttgcc
      721 ctcctccacc gcgagcgccg cgacctgctc gcgggcgtga tcggcggcac cgaggagacc
      781 accaccggcg tcgtccgcct ccgcgccctg gccgccgagg gcaagctcgg ctaccccatc
      841 gtcgccgtca acgaagccga caccaagcac atgttcgaca accgctacgg caccgggcag
      901 tccaccatcg acggcatcac ccgcgccacc aacatcctcc tcgccggcaa gaacctcgtc
      961 gtctgcggct acggttggtg cggcaagggc gtcgcttcga gggcccgcgg cctcggcgcg
     1021 caggtcatcg tcaccgaggt tcagcccctc cgcgccctcg aggccgtcat ggacggcttc
     1081 cgggtcatgc ccatcctcga cgccgccctc atcggcgaca tctttgtcac cgtcaccggc
     1141 gacatcaacg tcatcgacgc cccctccttc gaggccatga agaccggcgc cgtcctcgcc
     1201 aactccggcc acttcgacgc cgagatcaac ctcaaagccc tcgaaggcat gaccgaggcg
     1261 cgccgcccgc tccgcccctt cgtggaggaa ttcaccctcc aggacggccg caagctcgtc
     1321 gtcctcgccg aaggccgcct catcaacctc ggcgccgccg agggccaccc cgcctccgtc
     1381 atggacatgt ccttcgccaa ccaggcactc tgttccgagt acctcgtcgc gcaacacgcc
     1441 tcactcaaac ccgacgttta caacgtcccc cgcgacatcg acgacgaagt cgcccgcctc
     1501 aagctcgccg ccatgggcgt cgccatcgac accctcaccg aggagcagca ccgctacctc
     1561 aactcctggg agctcggcac gcagtagccg ccgctgcctc gttgccaacc accgttcccc
     1621 cggccatgat ccgagggcaa cgcgccgtgg ccacaagggg cgagcatggc cagtactgct
     1681 gacatcgccg tcatcggcgg ctccggcttc tacgacctcg aaggcctccg cgatgcgcgc
     1741 ccactcgagg tcgatacccc tttcggtgcc acctcctcgc cgatcctggt cggcgagctc
     1801 cacggtaagc gcgtagcctt catcgctcgt cacggcgagg gtcaccgcat cctcccagcc
     1861 gaggtcccct cgcgcgccaa cctctgggct ctcaagcacc tcggcgtgcg ccgcgtgctg
     1921 gcggtctccg ccgttggctc gctgcagcag caatatcacc cgctcgacgc cgtcgtcccc
     1981 gaccagctcc tcgaccgcac gcgagggcgc gcatcgacct acttcggtga cggcgtcgtc
     2041 gcccacatcg gcttcgccga ccccttctgc gacgaaaccc gcgaggcctt ggccgcggcc
     2101 tccgaggcag ccggcgtcac cacccatcgc ggtggcacgc tcgtggtcat cgaggggcct
     2161 gccttctcca cccgcgctga gtcccgcctc taccagtcct ggggcgccgc cgtcatcggc
     2221 atgaccgccc ttcccgaggc caagctcgcc cgcgaggccg agctctgcta tgcctccatc
     2281 tgctacgtca ccgactacga tgtatggcac gaccaggaag aagacgtcgg agtggagctg
     2341 gtcgtcgaga acatgcgccg caacatccag cacgcccgcg acaccgtcag ccgcaccatc
     2401 gcggccctgc cgatcgaggg cgcctgcgcc tgcggtgatg ccctcaacgg cgccgtgatc
     2461 accgcgcccg atgccgtccc tccggcgacg aggcaacgcc tcgccttcat gctcgaccgc
     2521 tactggggcg cccagtgacc gacgcgctct acggccccgc cgccgaggcg cgtccgcgct
     2581 tccgcgtccg gcgcgtcgtc gaccgccgcg tgctcgatgc ggcgctcagc gaggatcgcg
     2641 cgtacgccgc ctacgccctc ggccatctcg agcccggcct cttcgagcag tcgcgcttct
     2701 ggctcgccga aggcccgagc ggcagcggcg tgctcctcca cgcggatggt ctgctcgggc
     2761 gcaccaccgt ggtcgtcggc gaccctcacg ccgtcgatgc cctcgtcgcc ctccaccccg
     2821 gcacgcgcgg ctcttacctc gccaccggct ctcccgacca cctcgaggcc ctgcgccgca
     2881 cctacctcgt gctcggctcg ctcgagatgc gccgcatgtc cgtcaacgcc gccaccttcg
     2941 ccccgcgccc gtcacttcgg caccacgtgc ggcgcctcgg cggccatgac acccgcgcgc
     3001 tcaacatgct ctactccacc gagggcccgc cgacggcgta taccgccgac caggtgaacc
     3061 agtccgtcta ctacggc
//
LOCUS       DAORPG010000176         3070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3133_length_3070_cov_3.169486, whole genome shotgun sequence.
ACCESSION   DAORPG010000176 DAORPG010000000
VERSION     DAORPG010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3133_length_3070_cov_3.169486"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..336
                     /locus_tag="PLX85_04940"
     CDS             7..336
                     /locus_tag="PLX85_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008101284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HRC62550.1"
                     /translation="MTARILAFRDARDWMQFHNPKELAVAITAEAGELLQHFVWQQPE
                     QLEKRVQEKSAELKDEMADVAILLFELAHNLGVDLGQAMLDKIERNETRYPVAKARGS
                     NAKYNEL"
     gene            485..826
                     /locus_tag="PLX85_04945"
     CDS             485..826
                     /locus_tag="PLX85_04945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRC62551.1"
                     /translation="MVNSITSYFGPSHPLGIDIEAPYVPVVASRAGQVVFVGGDPCCS
                     YGLYVIIRHDGEYETRYAHLSQFSVQLGQWVDAGQQIGISGSTGRSTGAHLHFEVIYN
                     GSVINPLTVLP"
     gene            complement(923..1555)
                     /locus_tag="PLX85_04950"
     CDS             complement(923..1555)
                     /locus_tag="PLX85_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC62552.1"
                     /translation="MEITWLGHACVRIRTRQAIVLMDPFERSAGLDMGRPTADIVTVS
                     HDHPLHSYVAGVKGEPLVIAGPGEYEVQGVQILGVATSLRPEAEDAKAGRNVAYLMEA
                     EGLHVAQLGDIGVQPTASDAEPLVNADVLILPIGEGTLEPDQAARTVRMLEPSIAIPT
                     GYHANGADDPRLKAFLAAVGIEPEPPVAKLVVQGRGAGEGQRIVLLEPRG"
     gene            complement(1649..2164)
                     /locus_tag="PLX85_04955"
     CDS             complement(1649..2164)
                     /locus_tag="PLX85_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRC62553.1"
                     /translation="MEADAFSALYEAHAPRVYRYLLSRTSRPAEAEELTSRTFLNALT
                     HLEGYRGGRGGGADPFGSWLMSIAHNLLANWYRDRGRRPPTAPLDDALTIPTEGPDPQ
                     STLEMSEQVRRVREAVRALAPDRQELLAFKYVEGLTNAQIGERMGRSEGAVKALHHRT
                     LRQLQETLREP"
     gene            2339..>3070
                     /gene="hrcA"
                     /locus_tag="PLX85_04960"
     CDS             2339..>3070
                     /gene="hrcA"
                     /locus_tag="PLX85_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRC62554.1"
                     /translation="MEETRVLTRRRTEILGLVVHQYIDTAAPVSSRALVSRHGLSIST
                     ATIRNELAKLEEDGYIAQPHTSAGRVPLDRGYRLYVEELMAEEPIGVDEQRTIEHQFH
                     QAPAHLEEWLSLAAHVLATAVSNVAVITRPTNRVAQLRQVQLVHLHDETALLVAVMDD
                     GRVQQRMVLLSRSEDQDGLTARAMRLNARIANAEAATVRAVAEELTDEDDRRLAEVAA
                     ELIDEHRVAGETYLEGLRSALEQPEF"
BASE COUNT          501 a         1006 c         1071 g          492 t
ORIGIN      
        1 cccgacatca ccgcccgcat cctggctttt cgcgacgcac gcgactggat gcagtttcac
       61 aaccccaaag agctcgccgt ggccatcact gcggaagcgg gggagctttt gcagcacttt
      121 gtctggcagc agccggagca gctcgagaag cgtgtgcagg agaaaagcgc cgaattgaag
      181 gacgaaatgg ccgatgtggc catcttgctc ttcgaactcg cccacaacct cggcgtcgat
      241 ctcggccagg ccatgctcga caagatcgag cgcaatgaaa cgcgctaccc cgtggccaag
      301 gcccgcggca gcaacgccaa atacaacgag ctgtgagcga ctctgaaacc agtaaccggg
      361 gcattcctgc cccgaccgat gaggagccag gaatggcccc cctacagccc acgccgcaca
      421 gccgacggcc gccgctgcgg ccgcaggctc gagcacgaac gcggcgacgg gctggatctg
      481 gccggtggtc aacagcatca cctcctactt cggtccgagt cacccgctgg gcatcgacat
      541 cgaggcaccg tacgtgccag tcgtcgcctc gcgcgccggg caggtcgtct tcgtcggtgg
      601 cgacccgtgt tgttcctacg gcctgtatgt catcattcgc cacgacggcg aatacgagac
      661 gcgctacgcg cacctgtcgc aattcagcgt ccagctcggc cagtgggtag acgcaggtca
      721 gcagatcggc atcagcggca gcacgggtcg ttcaacgggc gcgcacctgc acttcgaggt
      781 catctacaac gggtcagtga tcaacccgct gacggtactg ccctagcccg cctgcgcacc
      841 cgtctcgggg catcaacgca cgccgagcac gcgcggatgg cgcccgcctc cgcgcttgtt
      901 cgtcgccagg gtcgggcgct tcttagccgc ggggctccag caggacgatg cgctggcctt
      961 cgcccgcgcc ccggccctgc acgaccagct tcgcgaccgg aggctccggc tcaatgccga
     1021 ccgcagcgag gaatgctttg agcctcgggt cgtcggcgcc attggcgtgg tagccggtgg
     1081 ggatggcgat cgacggttcg agcatgcgca ccgtgcgcgc ggcctggtcg ggctcaagcg
     1141 ttccctcacc gatgggcagg atcaggacgt cggcattgac gagcggctcc gcgtctgacg
     1201 cggtgggctg aacgccgatg tccccgagtt gggcgacgtg caggccttcc gcctccatca
     1261 ggtacgcgac attgcgtccg gccttcgcgt cctcggcctc tggtcgcagc gaggtcgcca
     1321 cgccgaggat ctgcacaccc tggacctcgt actcgccggg gccggcgatg accagcggtt
     1381 cgccctttac gccggcgacg tacgagtgca ggggatggtc gtgactcaca gtgacgatgt
     1441 cggccgtcgg ccgtcccatg tcgagcccgg cggagcgctc aaagggatcc atgagcacga
     1501 tcgcctgtcg cgtgcggatt cgcacacagg cgtgccccag ccaggtgatc tccacaggtc
     1561 gcctccgtca acggttcggg aagatgatca tatcggtggc ggccgtcagt cgccaccaga
     1621 agcgcctgtc ccggggccgg agggcgcctc agggctcgcg gagcgtctct tgaagctgcc
     1681 gcagcgtgcg gtggtggagc gccttcaccg cgccctcgct gcggcccata cgctccccga
     1741 tctgcgcatt cgtgagcccc tcgacgtatt tgaaggccag cagctcctgg cggtctggcg
     1801 cgagcgcgcg gacggcctca cgcacccggc gtacctgctc gctcatctcg agggtcgact
     1861 gtgggtctgg cccctcggtc gggatggtga gcgcatcgtc gaggggggcg gtcggaggcc
     1921 ggcggccgcg gtcccggtac cagttggcga gcaggttgtg ggcgatggac atgagccagg
     1981 agccaaaggg atcggcgccg ccgccccggc cgccgcggta cccctcgagg tgggtcaggg
     2041 cgttgaggaa cgtgcgtgag gtgagttcct ctgcttcggc cggacggctg gtgcgggaga
     2101 gcaggtaacg gtagacgcgc ggggcgtgcg cctcgtacag ggcggagaag gcgtcagctt
     2161 cgatgcgcgg gccggtggcg gcgccgcgcg cgtgtgggtt cgacagcggg gtgctcctgt
     2221 gtcggtggag tataggtgaa tgcgtttgga atgcgggcca gcgggcggga attgacccgg
     2281 ttcgccgtcg ctcggtacaa tcggggacgt agttagcact ctcgcgtcga gagtgctaat
     2341 ggaggaaacc agggtgctga cccggcgtcg aaccgagatt ctcgggctgg tcgtccatca
     2401 gtacatcgac actgccgcac cggtgagttc gcgtgcgctg gtgtcgcgac atggcctcag
     2461 catctccacg gccaccatcc gtaacgagct ggcgaagctc gaggaagacg gctacatcgc
     2521 gcagccgcac acctcggcgg ggcgcgtccc gctcgatcgc gggtaccgcc tgtacgtcga
     2581 ggagctcatg gcagaggagc ccatcggtgt cgatgagcag cgcacgatcg agcaccagtt
     2641 ccaccaggcc ccggcgcacc tcgaggagtg gctgagcctg gccgcgcacg tgctcgcgac
     2701 ggccgtctcc aatgtcgccg tgatcacgcg gcccacgaac cgcgtcgcgc agttgcggca
     2761 ggtgcagttg gtgcacctcc acgacgagac tgcgctgctg gtcgccgtga tggatgacgg
     2821 gcgcgtgcag cagcgcatgg tgctgttgag ccgcagcgag gaccaggacg ggctcacggc
     2881 gcgggcgatg cggctcaatg cgcggatcgc gaatgccgag gcggcgaccg tgcgcgccgt
     2941 cgccgaggaa ctgaccgacg aagacgaccg ccggcttgct gaagtggccg cggagctcat
     3001 cgatgagcac agagtggccg gtgagacata cctcgagggg ctgcgctcgg cgctcgagca
     3061 gccggagttc
//
LOCUS       DAORPG010000177         3065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3140_length_3065_cov_3.521262, whole genome shotgun sequence.
ACCESSION   DAORPG010000177 DAORPG010000000
VERSION     DAORPG010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3140_length_3065_cov_3.521262"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PLX85_04965"
     CDS             complement(<1..381)
                     /locus_tag="PLX85_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRC62555.1"
                     /translation="MTSDTGERMLPLEGVRVLSQAIVWAGPFGTMILSDLGADVIEVE
                     SIQHLNPTRAAVRHLPDLFMQSPTGAGYLNRDNSEGFWNRSATFNYAKRGHRSITLDL
                     LRPEGLELFYSLVRAKDIANNEDVR"
     gene            complement(381..1589)
                     /locus_tag="PLX85_04970"
     CDS             complement(381..1589)
                     /locus_tag="PLX85_04970"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRC62556.1"
                     /translation="MAAPLEGIRVLDLTQGVAGPFATKHFSDYGAEVIKVERPGQGDM
                     ARRLGPFPDDRPHLERSGMFLTLNTGKRSVTLDLKTATGQQILRRLAEDADIVVESFR
                     PGTLERLGLGPDVLREVNPRASLVRVSNFGQTGPYRDFNADDMLMYAMGGVLAVTAGE
                     GREPVKIGLYAPLFLAGNVVSAFVMGAFFGARRRGIGERADLSIHEILASSMDRGGTN
                     LVSYGYSGALYFEPRQPQRSSALPAGVYPCLDGYIHILCQLQWWSRFCQLIERPDLVN
                     DDHLAPNLLDINYAPEVDAIFFPWLLQRTKQEAMEAGQAVGLPIAAINTMQDVLNDPQ
                     LNARGFFVDVEHPAAGAVRLPGVPLRMSGTPGELRRAPLLGEHTFEILTERLGYDPAD
                     VARLEQQGVI"
     gene            1920..>3065
                     /locus_tag="PLX85_04975"
     CDS             1920..>3065
                     /locus_tag="PLX85_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRC62557.1"
                     /translation="MSARNPVVRRNVALLMGGKLIGLLAVLMLIHWLTPETASAAEGE
                     PDPLVTVNALNTVWVLVAAFLVFGMQVGFVMLEAGFARERESVNILVEGIADTCICGV
                     LFWAIGFAFMFGEGNGFIGTHGFFLHGLPDTYGTTGVPLMAYWVFQFAFADTASTITS
                     GAMVGRCGFVGDIVYSIGVTGFIYPIIGHWAWGPDGWLALRGFRDFAGSTVVHTIGGT
                     ISLAGALALGPRLGRVFRRDGGGPMPPHNLLLGAIGGLMLWFGWYGFNPGSTLSALDM
                     QGIGRVSFNTTMAACTGGLGALFIGYARTRKWDLGLTVNGFLGGLVAITCPCYWVDAQ
                     GAFIIGLVAAPIVVGGLDLLEYLRIDDPIGAVPVHMFAGIWGTLSLGL"
BASE COUNT          489 a          958 c         1089 g          529 t
ORIGIN      
        1 gcggacgtct tcgttgttgg cgatgtcctt ggcgcggacg agcgaataga acagctccag
       61 gccctcggga cgcagcaggt cgagcgtgat gctgcggtgg ccgcgcttcg cgtagttgaa
      121 ggtcgcgctg cggttccaaa agccctcgct gttgtcgcgg ttcaggtagc cggccccggt
      181 gggactctgc atgaaaaggt caggcaggtg ccgcacggcg gcgcgcgtcg ggttgagatg
      241 ttggatcgat tcgacctcga tcacgtccgc gccgaggtca gagaggatca tggtgccgaa
      301 ggggccagcc cagacgatgg cctgggagag gactcggacg ccctcgaggg gcaacatgcg
      361 ctcgccggtg tcgctggtca tcaaatgact ccttgctgct cgaggcgggc gacgtcggcg
      421 gggtcgtagc cgaggcgctc agtcaggatc tcgaaggtgt gttcaccgag gagcggcgct
      481 cgccgcagct cgcccggcgt gccggacatg cgcaacggga cgcccggcag gcggaccgcg
      541 cctgccgccg ggtgctcgac gtcgacgaag aacccgcgcg cgtttagctg cgggtcattc
      601 agcacgtcct gcattgtgtt gatcgcggcg atcggcagcc cgaccgcctg acccgcctcc
      661 atggcctcct gcttcgtccg ctggagcagc caggggaaga agatcgcgtc cacctcgggg
      721 gcgtagttga tgtcgagcaa gtttggggca aggtggtcgt cgttgaccaa gtcgggccgc
      781 tcgatgagct ggcagaaccg cgaccaccac tgcagctggc aaaggatgtg aatgtagcca
      841 tcgaggcacg ggtacacgcc cgccgggagc gccgagctgc gctgcggctg gcgaggttca
      901 aagtacaggg cgcccgagta gccgtaggag acgaggttgg tgccgcctcg atccatcgag
      961 ctggcgagga tttcgtggat ggagaggtcg gcacgctcgc cgatcccgcg acggcgggcg
     1021 ccaaagaagg cccccatcac gaaggcggag acgacgttgc ccgcgaggaa gagcggggcg
     1081 tagaggccga tcttcaccgg ctcgcggccc tcgcccgcag tgacggcgag gacgccgccc
     1141 atcgcgtaca tgagcatgtc gtcggcgttg aagtcacggt agggcccggt ctggccgaag
     1201 ttggacacgc ggacgagcga cgcccgcggg ttgacctcgc gcaggacatc gggaccgagg
     1261 ccgagccgtt cgagcgtgcc gggccggaaa ctctcgacga cgatatcggc gtcctcggcg
     1321 agacggcgca ggatctgctg gcccgtcgcg gtcttcaggt cgagcgtgac gctgcgcttc
     1381 ccggtattca gggtgaggaa catgccgctg cgctcgaggt gtggccgatc gtcggggaac
     1441 gggccgagcc gcctcgccat atcgccctgc ccgggccgct cgactttgat cacctcggcg
     1501 ccgtagtcgg agaagtgctt ggtcgcgaag gggccggcca cgccctgggt caggtccagg
     1561 acgcgaatgc cctcgagtgg tgccgccatc gaaccccctc cggcgctgct gcgggattgc
     1621 gtatgtcgtg gaagtcagac tcgtataccg ctgcgccggt tggcccgcaa gccgagccgc
     1681 cgaatccccg cccgaaggga ccacacgacg gggcccctcg ttttcggatt cccgaaacat
     1741 tcaggacaca ctcaaataac caggtcgaaa caccgtttca acgggcaaga aatgtgacgc
     1801 acctacggtg gcctcagtca tccgggcggc gcgaagcgtc gccggggcag tgctggaaga
     1861 gggatgaatg gaggcgcact ttggaagcgg aaccacgacc gctcgttgaa cgattggcga
     1921 tgagcgcgcg caacccggtc gtgcgtcgca acgtcgcact cctcatgggg gggaagctca
     1981 tcgggctcct cgccgtactc atgttgatcc actggctcac gccggagacc gcgagtgccg
     2041 cggagggcga accggacccg ctggtgacgg tgaacgcact gaacaccgtg tgggtgctcg
     2101 tggcggcatt cctggtgttc ggaatgcagg tcggcttcgt catgctcgaa gccgggttcg
     2161 cccgcgagcg cgagtcagtg aacatcctcg tcgagggcat cgccgacacc tgcatctgcg
     2221 gcgtcctgtt ctgggcgatc ggcttcgcgt tcatgtttgg tgaaggcaac ggcttcatcg
     2281 gaacgcacgg attcttcctg cacggcttgc cggacaccta cggcacgacg ggcgtcccct
     2341 tgatggcgta ctgggtcttc cagttcgcgt tcgcggacac cgcctcgacg atcacctccg
     2401 gcgccatggt gggccgctgt gggttcgtcg gagacatcgt ctacagcatc ggggtgacag
     2461 gcttcatcta ccccatcatc ggccactggg cgtggggtcc cgatggatgg ctggcgctgc
     2521 gaggcttccg agacttcgcc ggatcgacgg tcgtgcacac gatcggcggc acgatctccc
     2581 tggcgggtgc gttggcgctc ggaccgcggc tgggccgggt cttccgacgg gacgggggcg
     2641 gcccgatgcc cccacacaac ctgctgctgg gggccatcgg cggcctcatg ctctggttcg
     2701 gctggtacgg cttcaaccca ggaagcacct tgtccgcgct cgatatgcag gggatcggcc
     2761 gcgtgtcctt caacacgacg atggcggcct gcactggcgg gctgggcgcg ctcttcatcg
     2821 ggtatgcccg gacgcgaaag tgggacctgg gtctgaccgt gaacggcttc ctgggtgggc
     2881 tggtcgcgat cacctgcccc tgttactggg tcgacgcgca gggcgcgttc atcatcggtc
     2941 tcgtggcggc gcccatcgtg gtgggcgggc tcgacttgct cgagtacctg cgaatcgacg
     3001 acccgatcgg agccgtcccg gtgcacatgt tcgcggggat ctggggcacg ctctccctcg
     3061 ggctg
//
LOCUS       DAORPG010000178         3049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3172_length_3049_cov_2.763193, whole genome shotgun sequence.
ACCESSION   DAORPG010000178 DAORPG010000000
VERSION     DAORPG010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3172_length_3049_cov_2.763193"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PLX85_04980"
     CDS             complement(<1..639)
                     /locus_tag="PLX85_04980"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRC62558.1"
                     /translation="MTTTASGRRFEAVCFDLDETLIDSAVAWRAGFLDAFALEVAPRY
                     PELGATAEPLLELQPLLVAEVQARGGRWGDFVIRDAVRKFFRAHGIEDDRSADRTTEA
                     YEAAWPRHITLFPDALSALDAAAELGPLALITNGPSTGQRLKLERTGLLDRFEVLAIS
                     EEVGAEKPDLEIFTHTLEGLGIHAAAAVHIGDNLHMDIRGARDAGMTAVWLNR"
     gene            complement(623..958)
                     /locus_tag="PLX85_04985"
     CDS             complement(623..958)
                     /locus_tag="PLX85_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62559.1"
                     /translation="MTDYRFEREARTPYSEVYTIDDGEHSLGRVDIHYTGSATYATLA
                     VHQTLDEDGVQALIAAIDERIVSTADPYREDFVVTVWRGEEFGVFADESDFDEEDEDA
                     EDDEDDDDR"
     gene            complement(1071..1241)
                     /locus_tag="PLX85_04990"
     CDS             complement(1071..1241)
                     /locus_tag="PLX85_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62560.1"
                     /translation="MNATATRATELFANRRLRRVALAGPRCPDCAGPLIYGEGCHLCP
                     VCGYSGCSPTRR"
     gene            1344..1532
                     /locus_tag="PLX85_04995"
     CDS             1344..1532
                     /locus_tag="PLX85_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62561.1"
                     /translation="MGKLLTLMLLLAVVALGVAFLYQPKNKRSRFATFTKRVRTVAYA
                     YVAAILISAILRLAFGWA"
     gene            complement(1540..2535)
                     /locus_tag="PLX85_05000"
     CDS             complement(1540..2535)
                     /locus_tag="PLX85_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRC62562.1"
                     /translation="MAVPTAPPSARPRRIAIDWRLVAGLLRRATVNFVEDRCTVLAAA
                     ISYFALFSLFPLVLLAAAVFGIVLRDPDVQTRVLNAVVDAIPVEAPSVSNSLRALADL
                     GPTLSVVATIGTLWTASALAAAIRNALDIVFDVRRPRALLQAKILDYLVLIGVGALFL
                     TSLALTTAWRVAQTQADVRWGLLDGNLRWLWDLGAVLIPAVMTFLMFLLLYRALPHGR
                     TRLLHIWPGALLAAVAFELAKALFATYLANFANYDVVYGSLGGVIALLFWVYVSANIL
                     LFGAEVAAEVSHVMRGEPRRGHTPGDEGDWRSSLLTLLRGLVLAPGEGEERRRRW"
     gene            2638..2802
                     /locus_tag="PLX85_05005"
     CDS             2638..2802
                     /locus_tag="PLX85_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62563.1"
                     /translation="MSRHHSSHEQTQYRRIVVKVGSNLLTGGGDRLDAHAMAGLVQQI
                     ASVRARGPQR"
BASE COUNT          500 a          898 c         1212 g          439 t
ORIGIN      
        1 gcggttgagc caaacggcgg tcatgccggc gtcgcgggcg cctcggatgt ccatgtggag
       61 gttgtcgccg atgtgcacgg ccgcggcggc gtggataccg aggccctcga gggtgtgggt
      121 gaagatctcg agatcgggct tctcagcgcc gacttcctcg gagatggcga gcacctcgaa
      181 acggtcgagg aggccggtgc gttcgagctt gaggcgctgg ccggtggagg gcccgttggt
      241 gatgagggcg agcgggccga gctcggcggc ggcgtcgagg gctgagagcg catccgggaa
      301 gagggtgatg tgccggggcc aggcggcctc gtacgcctcc gtggtgcggt cggcggagcg
      361 gtcgtcctcg atgccgtgcg cgcggaagaa cttgcgcacg gcgtcgcgga tgacgaagtc
      421 accccagcgg ccgccgcgcg cctgtacctc ggcgacgaga agcggttgca gctcgaggag
      481 cggctccgcc gtcgccccga gctcgggata gcgcggtgcg acctcgagcg cgaaggcgtc
      541 gaggaagccg gcgcgccagg cgacggcgga gtcgatcagg gtctcgtcga ggtcgaagca
      601 gacggcctcg aaacggcgtc cgctagcggt cgtcgtcatc ctcgtcgtct tcggcgtcct
      661 cgtcttcctc gtcgaagtcg gactcgtcgg cgaagacacc gaactcctcg ccgcgccaga
      721 cggtgaccac gaagtcctcg cggtaggggt cggcggtgga gacgatgcgc tcgtcgatgg
      781 cggcgatgag ggcctggacg ccgtcctcgt cgagggtctg gtggacggcg agcgtggcgt
      841 aggtggcgga gccggtgtag tggatgtcga cgcggccgag ggagtgctcg ccgtcgtcga
      901 tggtgtagac ctcggaatat ggagtgcggg cttcgcgctc gaagcggtag tcggtcacgg
      961 tggggtcctc tcgaggggtg tcgtgttgtg tcgcgttcag gctacacgag ggcgggcgct
     1021 ggcgcgcctc gagaggatgc agagtggagc gtgtacgagt gccgccgagg tcagcgccgg
     1081 gtgggtgaac agccggagta accgcagacg gggcagaggt ggcagccctc gccgtagatg
     1141 aggggcccgg cacagtccgg gcagcgcggc ccggcgaggg caacgcggcg caggcggcgg
     1201 ttcgcgaaca gctcggtggc gcgggtggca gtggcgttca tcgggcggct ccgagagaac
     1261 atgcgttctg tatgcctcga gggtagacta gaacgcatgt gcttgtcaac acggtagcgt
     1321 cgtcagtcgg taggatccgg cacatgggca agctgctgac gttgatgctg ttgctcgccg
     1381 tggtggccct cggcgtggcg ttcctgtacc agccgaagaa caagcgctcg cgcttcgcga
     1441 cgttcacgaa gcgggtgcgg acggtggcgt acgcctatgt ggcggcgatc ctgatctcgg
     1501 cgatcctgcg gttggcgttc ggctgggcgt aactcggcgc taccagcggc gccgtcgctc
     1561 ctcgccttcg ccgggggcga gcaccaatcc ccgcaacaac gtgagcagcg aggagcgcca
     1621 gtcgccctcg tcgccggggg tgtggccgcg gcggggctcc ccgcgcatga catgtgagac
     1681 ctcggcggcg acttccgcgc cgaagaggag gatgttggcg ctcacgtaga cccagaacag
     1741 cagcgcgatc acgcctccga gggagccgta gacgacgtcg tagttcgcga agttggcgag
     1801 gtaggtcgcg aagagggcct tggcgagctc gaaggcgacg gcggcgagga gggcgcccgg
     1861 ccagatatgg agcagacggg tgcggccgtg ggggagcgca cggtagagca gcaggaacat
     1921 caggaacgtc atcaccgccg ggatgaggac cgcgcccagg tcccacaacc agcgcaggtt
     1981 gccatcgagg aggccccagc gcacgtcggc ctgtgtctgg gcgactcgcc aggcagtggt
     2041 gagcgcgagc gaggtgagga agagcgctcc gaccccgatc agtacgaggt agtcgaggat
     2101 cttcgcttgg agcagggcgc gcgggcgccg gacgtcgaag acgatatcga gggcgttgcg
     2161 gatggcggcg gcgagagcgc tcgcggtcca caaggtgccg atcgtcgcga cgacggagag
     2221 ggtagggccg aggtcggcga gggcgcgcag cgagttcgac acgctgggcg cctcgacggg
     2281 gatggcgtcg accacggcgt tgagtacccg cgtctggacg tcggggtcgc gcagcacgat
     2341 accgaagacg gcggcggcga gcaggacgag cgggaagagc gagaagaggg cgaagtagga
     2401 gatggcggcg gcgaggacgg tgcagcggtc ctcgacgaag ttgacggtgg cgcggcggag
     2461 gagtccggcg acgagccgcc agtcgatggc gatacgccgc gggcgggcgg agggaggcgc
     2521 ggtgggtacg gccatgcccg tcgcgtccct ttcctgcggc gatcgctcga cgatggcccc
     2581 ctgctcctcc atcctaaagc aggtctcgaa ataacgagtc gccacgggcg cgagagcatg
     2641 agcaggcacc actcctccca cgagcagacg cagtaccggc gcatcgtcgt gaaggtgggc
     2701 agcaacctgc tgaccggcgg tggcgaccgc ctcgacgcgc acgcgatggc ggggctggtg
     2761 cagcaaatcg cctcggtgcg ggcgcgaggg ccacagcgat agagcagcca gaaggcgaag
     2821 aaatcgaccg gcgagcgatc ggcgacgaag ccgccggcgg cgcgaatggc ctggtgctcg
     2881 agctgacgct gctcttccca gagctcgatg accagatcgc cgagctcggc gagatcgagc
     2941 gtgtgcagct cgagaccacg ctcgatgcgg gtgcgcatgc cttcggggac gtacggcacc
     3001 tcgagacgcg tcgcgatcgt cccgatcgcc acgttcgcga gccccgtgg
//
LOCUS       DAORPG010000179         3040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3191_length_3040_cov_3.075377, whole genome shotgun sequence.
ACCESSION   DAORPG010000179 DAORPG010000000
VERSION     DAORPG010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3191_length_3040_cov_3.075377"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..450
                     /locus_tag="PLX85_05010"
     CDS             151..450
                     /locus_tag="PLX85_05010"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HRC62564.1"
                     /translation="MATVSVLLTLYLPGMQSLKEKRAVVRSLVARFRSRLDLTAAEVG
                     DQDRLQHAQVGFAVVSGDLGTARRLADEARRFADAELLGRAEVVATEVAETVIEG"
     gene            complement(532..660)
                     /locus_tag="PLX85_05015"
     CDS             complement(532..660)
                     /locus_tag="PLX85_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62565.1"
                     /translation="MDMETRRYYSRLVLTGRRSGPTMREAERDLARAYSGIFTPIA"
     gene            complement(983..1399)
                     /locus_tag="PLX85_05020"
     CDS             complement(983..1399)
                     /locus_tag="PLX85_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRC62566.1"
                     /translation="MPEVGARASRELVVTPELVRAYAEITGDYNPLHFDEEFVAATRF
                     GRLMAQGGIATGLLHALVAMDLPGPGSVFSRQRWSFPRPVYIGDTIRAEGTILSANTR
                     RRFYEMAFIVTNQAEETVLEGEATVYQAERRSNAEV"
     gene            complement(1423..1629)
                     /locus_tag="PLX85_05025"
     CDS             complement(1423..1629)
                     /locus_tag="PLX85_05025"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HRC62567.1"
                     /translation="MVGKIIDMFRREDTAVDPVCGMTVNKKSPGGGTYEFEGTTYYFC
                     GRGCRLEFEEDPRGYLSGEKHVEM"
     gene            complement(1698..>3040)
                     /gene="rimO"
                     /locus_tag="PLX85_05030"
     CDS             complement(1698..>3040)
                     /gene="rimO"
                     /locus_tag="PLX85_05030"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRC62568.1"
                     /translation="GPEQAGPEQTGPADVVKVALITLGCARNEVDSQELAGRLAADGF
                     ELVDEPEQADTVVVNTCGFIDAAKDESIEVVRALDASRRPGQQLVVVGCMTQIAEAEL
                     RAAVPAVDATFGVEAWDEVALSLGPAVETYDIPQPAAAGLLATGPSAYLKISDGCDRP
                     CTFCIIPTIKGRMHSDAADRLLREARWLAAGGAKELVLVAQDSTAFGEDRGERDGLAT
                     LLEQLAEAVPDVPWIRLMYAYPGRVTRRLAETMARLPNVVPYLDMPLQHGSDAVLRRM
                     KRPSLRKARESIEAIRTAMPDAVLRTTFIVGYPGETEREFRELLAFIEEQRFDHVGAF
                     TYSPQPGTPAASEAGQVPERVKQKRYARLMELAQGIAHERNRAMVGRELDVLVESDAP
                     AQSEAGGPVVVGRTYRDAPEVDGLAFLKGEYPSGALVRARVDGALPYDLLCSPV"
BASE COUNT          451 a          988 c         1116 g          485 t
ORIGIN      
        1 gccgtcgagc ccggtccccg gctccccacg gcggggccga ctctgccgcg cagcggcccg
       61 ggcgcgccgc ctcgcattcc agggagcgga cctccgagga gcgggccacc tggtggcggc
      121 ggcgcgccgc ggttcccgta ggccagctcg atggcgaccg tgagtgtcct gctgacgctc
      181 tacctgcccg gcatgcaatc gctcaaggag aagcgggccg tcgtgcgctc gcttgtcgcg
      241 cgctttcggt cgcgcctcga cctgacggcc gccgaggtgg gagaccagga ccggctccag
      301 cacgcgcagg tggggttcgc ggtcgtctcg ggtgacctcg ggacggcccg ccggctggcg
      361 gacgaggcgc gacgcttcgc ggacgcggag ctgctcggac gcgcggaggt cgtggcgacc
      421 gaggtcgcgg agaccgtcat cgagggctag gcgtggcacg tcaacggcga gcagacacac
      481 gaaaggcgcc cggggggagg gcgcctttcg tcgagggggg ctgcgctggt gttaggcgat
      541 cggcgtgaag atgccggagt acgcccgtgc gaggtcgcgc tcggcctcgc gcatggtggg
      601 accactgcgc cgccccgtga gtacgagccg gctgtagtac ctccgcgttt ccatgtccat
      661 cgttcggtcc tttcctcctg tacggcccgg gccttccgcc cttgaccgtc ctcgtgcatt
      721 cattgtcgat ctcctcgaaa cctcctgcaa gtgattgtga gaacaatcac gtgaaatgga
      781 ggttaactcc ctgtgaaccg ctggctggct cgttcgcgac cgatccgatg ggcgttcaca
      841 ggcgaggggg agcgaggatc tgacaacccc tgcacaggta ataacaccgc ggcagaggaa
      901 aagttaccct acaggggtat ggttctccga gaaccccctg gcacgctcgt gtccgcgccg
      961 tcccagggtc cgacgcgcgg gttcagacct cggcgttcga ccgccgctcc gcctgataga
     1021 cggtcgcctc gccctcgagg acggtctcct cggcctggtt ggtcacgatg aacgccatct
     1081 cgtagaaccg acgccgggtg ttcgcactga ggatggtccc ttcggcgcga atggtgtctc
     1141 cgatgtacac cggacgaggg aagctccagc gctggcggct gaagacactg cccggccccg
     1201 gcaggtccat cgcgacgagt gcgtgcagca gaccggtcgc gatgccgccc tgggccatca
     1261 gccgcccgaa ccgagtcgcc gccacgaact cttcgtcgaa gtgcagcggg ttgtagtcgc
     1321 cggtgatctc ggcgtacgcg cgcaccagct cgggcgtcac gaccaattcg cgtgacgcgc
     1381 gcgctccgac ctcgggcacg ggcgatcctt tcgaggcggc cgctacatct cgacgtgctt
     1441 ctcgccgctc aagtagccgc gaggatcctc ttcgaactcc agccgacagc cacggccgca
     1501 gaagtagtag gtcgtgccct cgaactcgta ggtgccgccg ccggggctct tcttgttgac
     1561 cgtcatgccg cagacagggt cgacggcggt gtcctcgcga cgaaacatgt cgatgatctt
     1621 gccgaccatg gcgcacctcc cagccgtgat gggtgcagca tacgcctcgc gacagcgggc
     1681 gcggcagtga ggactcgcta caccggcgag cagagcaggt cgtacggcaa cgcgccgtcc
     1741 acccgcgcgc ggacgagggc gcccgagggg tattcgccct tgaggaaggc gaggccatcg
     1801 acctcggggg cgtcgcggta ggtgcggccg acgacgacgg ggccgccggc ctcggactgc
     1861 gcgggagcgt ccgactccac gaggacgtcg agctcgcgac cgaccatggc tcggttgcgt
     1921 tcgtgcgcga tgccctgggc gagctccatg aggcgagcgt agcgcttctg cttcacgcgc
     1981 tcggggacct ggcccgcctc ggacgcggcg ggggtgccgg gctgtggcga gtaggtgaac
     2041 gcgccgacgt ggtcgaaccg ctgctcctcg atgaaggcga gcagttcgcg gaattcacgc
     2101 tcggtctcgc cgggatagcc gacgatgaag gtcgtgcgga ggacggcatc gggcatcgcg
     2161 gtgcgaatgg cctcgatgct ttcgcgcgct ttgcggaggc tcgggcgctt catgcggcgg
     2221 agcacggcgt cggaaccgtg ctggagcggc atgtcgaggt aagggacgac gttggggagg
     2281 cgggccatcg tctcggcgag gcggcgggtg acgcggccgg ggtaggcata catgagacgg
     2341 atccagggca cgtcggggac ggcttcggcg agttgttcga ggagggtcgc gaggccgtcg
     2401 cgctcacctc gatcttcacc gaaggccgtc gagtcctgcg cgacgaggac gagctccttc
     2461 gcgccgccgg cggcgagcca gcgcgcctcg cggaggaggc ggtcggcagc atccgagtgc
     2521 atgcggccct tgatcgtcgg gatgatgcag aaggtgcagg ggcggtcgca gccgtccgaa
     2581 atcttgagat aggcgctcgg gccggtggcg aggaggccgg ccgcggcagg ctgggggatg
     2641 tcgtacgtct cgacggcggg gccgagactg agggcgacct cgtcccaggc ctcgacgccg
     2701 aaggtagcgt cgacggcggg gacggccgcg cggagctcgg cctccgcgat ctgcgtcatg
     2761 cagccgacga cgacgagctg ctggccgggg cggcgggagg cgtcgagggc acggaccacc
     2821 tcgatggact cgtccttggc cgcatcgatg aagccgcagg tgttcacgac gacggtgtcg
     2881 gcctgctcgg gctcgtcgac cagttcgaag ccgtccgcgg cgagccggcc cgccaactcc
     2941 tgggagtcga cctcgttgcg cgcgcagccg agggtgatga gagcgacttt cactacgtct
     3001 gccggtccag tttgttccgg tcctgcttgt tccggtccag
//
LOCUS       DAORPG010000180         3037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3199_length_3037_cov_3.368545, whole genome shotgun sequence.
ACCESSION   DAORPG010000180 DAORPG010000000
VERSION     DAORPG010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3199_length_3037_cov_3.368545"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PLX85_05035"
     CDS             <1..607
                     /locus_tag="PLX85_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRC62569.1"
                     /translation="DAMFLQCGLQPGERFLVHAAGSGVGTAAVQMASVVGCETFGTAG
                     SDEKLAKAAELGLDVGVNYHDADFAEVIAERTGGRGVDVILDVIGAEYWARNLQSLAP
                     RGRMVIVGTMSGSKLEVDLGALMGKRLRVHGTMLRSRSLEEKATLTQEFARRMLPLFA
                     SGRLVPVIDAVYPLEEVAEAHRHMEANANFGKIVLQVGDGD"
     gene            763..1872
                     /locus_tag="PLX85_05040"
     CDS             763..1872
                     /locus_tag="PLX85_05040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC62570.1"
                     /translation="MDLGLTETQELLKNAAREFLENECPESHVREMEEDPQGYSPQLW
                     NKMAEQGWQGLMIPEQYGGAGLDFLDLCVLLEEFGRALVPGPFLSTVLGGVVPLLVAG
                     SEEQKQACLPLIASGEAIFTLALTEPSARFDEQGVQAQATVSGNQVTISGTKLFVPDA
                     NVATHMVVAARSAGGVTLAIVPTNQAGVQVRQLLTIARDKQCEVVLNNATGELLGQEG
                     QGWAAVQEILNRYAVGECAYLVGLAQMDFEISVDYAKERVQFGRPIGSFQAIQHKCAD
                     MVTDVDGSRFIMYKAAWSLNTDQSDQQMAVNMAKAWTSEATRRVVAHGQQIHGGIGFT
                     KDYKIQLFYRRQKRSELAWGDGDHHRELVAQQLGI"
     gene            complement(1973..2374)
                     /locus_tag="PLX85_05045"
     CDS             complement(1973..2374)
                     /locus_tag="PLX85_05045"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRC62571.1"
                     /translation="MAPVEHLNPEGMHHNPAFSQAVVVSGPSRTVYVGGQNAVDAAGH
                     IVGEDIETQTRQAFANLKRVLGAAGGGIEHVVKWTIYIVAGQPLAPGFAVFQQEWGAR
                     PNPPAITGLFVSALANPAFLVEIDAVAVIPE"
     gene            complement(2445..>3037)
                     /locus_tag="PLX85_05050"
     CDS             complement(2445..>3037)
                     /locus_tag="PLX85_05050"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HRC62572.1"
                     /translation="SWKPFAAVAAAIAAGLVLMWGVNLQRTIDRQAENNATIAAVVEA
                     SAKQIQQLASAGVSMQASEDLRQQLQTAVADQETVIAISADPRAQISDLDATSAGHGA
                     GARFLWSGAAGAGVLIARSLPDLPLDSVYQVWVDDGASLVSAGTFLPDERGGAQKVVR
                     PASNMGVPLRVSVSVVPAGGATTIGPLVVLSGAVNR"
BASE COUNT          505 a          948 c         1125 g          459 t
ORIGIN      
        1 cgacgcgatg ttcctccaat gcgggctgca gccgggcgag cgcttcctcg tgcacgccgc
       61 gggctccggg gtggggacag cggcggttca gatggcctcg gtcgtcggct gtgaaacctt
      121 cgggaccgcc ggcagcgacg aaaagctcgc gaaggccgcg gagctgggcc tggacgtggg
      181 tgtgaactac cacgacgcgg acttcgccga ggtgatcgcc gagcgcacgg gcggccgcgg
      241 cgtggacgtg atcctcgacg tcatcggggc tgagtactgg gcgcgcaatc tgcagtcgct
      301 ggccccgaga gggcgaatgg tgatcgtcgg gacgatgagt ggctcgaagc tcgaggtcga
      361 cctcggcgcg ctcatgggga agcggctgcg tgtgcacggc acgatgctgc ggtcgcggtc
      421 tctcgaggag aaagccacgc tcacgcagga gtttgcgcgg cggatgctgc cgctgttcgc
      481 ttcggggcgg ctggtcccgg tcatcgatgc ggtgtatccg ctcgaggaag tggccgaggc
      541 acaccgacac atggaggcga acgcgaactt cgggaagatc gtgctgcagg tgggggatgg
      601 cgactgacca taacggaagc gggaaaaaat agtcgccgcg catctaggcg tggagtcccc
      661 cgatgtgtat aaaggggaac gagaattacg gtcagcatcg agagtcccct catccgaccc
      721 ctgggcgggg cgaacagagg acttagtgga aaggacgtga ggatggacct gggactgact
      781 gagacgcagg agctgctcaa gaacgcagcg cgtgagttcc tcgagaacga gtgccccgag
      841 tcgcacgttc gcgagatgga agaggacccg caggggtact cgccgcagct gtggaacaag
      901 atggcggagc agggctggca ggggctgatg atccccgagc agtacggcgg cgccggcctc
      961 gacttcctcg acctgtgcgt gctgctcgag gagttcgggc gcgcgctggt gcccggcccg
     1021 ttcctttcga ccgtcctcgg cggcgtggtg ccgctgctcg tcgccggctc cgaggagcag
     1081 aagcaggcgt gcctgccgct gatcgcctcg ggtgaggcga tcttcacgct ggcgctgacc
     1141 gagccctcgg cccggttcga cgagcagggc gtgcaggcgc aggccaccgt gtccggcaac
     1201 caggtcacga tcagcggcac gaagctgttc gtcccggacg cgaacgtcgc gacgcacatg
     1261 gtcgtggcgg cccgctccgc gggtggcgtc acgctggcca tcgtcccgac gaaccaggcg
     1321 ggcgtgcagg tgcggcagct gctgactatc gcgcgcgaca agcagtgcga ggttgtgctg
     1381 aacaacgcga ccggcgagct cctcggccag gaaggccagg gctgggcggc cgtgcaggag
     1441 atcctgaacc gctacgcggt cggcgagtgc gcgtacctcg tcgggctggc gcagatggac
     1501 ttcgagattt ccgtggacta cgccaaggag cgcgtgcagt tcggccgccc gatcggctcg
     1561 ttccaggcga tccagcacaa gtgcgccgac atggtcaccg acgtggacgg ctcgcggttc
     1621 atcatgtaca aggcggcctg gtcgctgaac accgaccaga gcgaccagca gatggccgtg
     1681 aacatggcga aggcgtggac gtccgaggcg actcggcgcg tggtcgcgca cggccagcag
     1741 atccacggtg gtatcggctt caccaaggac tacaagatcc agctgttcta ccgccgccag
     1801 aagcgctctg agctggcgtg gggcgacggc gaccaccacc gcgagctggt ggcgcagcag
     1861 ctcggcatct agttccaggt ccgagtcccg aacacaagag gccgcccgca agggcggcct
     1921 cttcgtttga agcgccgcag ggtggcctcc gagcctgacc gttggggagc ggctactcgg
     1981 ggataacggc gacggcgtcg atctcgacga ggaaggcggg attcgcgagc gcggagacga
     2041 acagcccggt gatcgcgggc gggtttggcc tggcgcccca ctcctgctga aacaccgcga
     2101 agccgggcgc gagcggctgc ccggcgacga tgtagattgt ccacttcacc acgtgctcga
     2161 tcccgccgcc tgccgctccc agcacccgct tgaggttcgc gaacgcctga cgcgtctgcg
     2221 tctcgatgtc ctcacccacg atgtgaccgg cggcgtcgac ggcgttctgg ccacccacgt
     2281 agaccgtgcg agacggtccc gaaaccacga cggcctgact gaaggccggg ttgtggtgca
     2341 tgccctcggg gttcaggtgt tcgaccggcg ccatgatctg cctcctcgcg acgcgcgaat
     2401 cgaggatagg ccatgcgtcg cggtcgagga gcggtacggt ctcgctagcg gttgacagcg
     2461 ccgctgagta cgacgagcgg gccgatcgtg gtggcgccgc cagcggggac gaccgaaacg
     2521 gacacgcgca gagggacgcc catgttgctc gcggggcgaa cgaccttctg cgcgccgccc
     2581 cgttcgtcgg ggaggaaggt gccggcggag acgagcgagg cgccgtcatc gacccagacc
     2641 tggtagacgc tgtcgagcgg aaggtcgggc aggctgcgtg caatgaggac gcccgcgccg
     2701 gccgcgccac tccagaggaa gcgcgcgccg gccccgtggc cggcagaggt ggcgtcgaga
     2761 tcactgatct gggcgcgcgg gtcggcgctg atcgcgatca cggtctcctg gtcggcgacc
     2821 gcggtctgga gctgctgccg gaggtcctcg gacgcctgca tgctgacgcc ggccgaggcg
     2881 agctgctgaa tctgcttcgc gctggcctcg acgacggcgg cgatggtggc gttgttctcg
     2941 gcctggcggt cgatggtgcg ctgcaagttg acgccccaca tcaggacgag tccggcagcg
     3001 atggcggcgg ccaccgccgc gaacggcttc caggagc
//
LOCUS       DAORPG010000181         3035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3201_length_3035_cov_3.808725, whole genome shotgun sequence.
ACCESSION   DAORPG010000181 DAORPG010000000
VERSION     DAORPG010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3201_length_3035_cov_3.808725"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PLX85_05055"
     CDS             <1..436
                     /locus_tag="PLX85_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC62573.1"
                     /translation="KIVGLEMGADDYVTKPFNPREVVARVKAALRRREWDRSDDGQRG
                     AVSVGDVTLDPSSRDVTVGETRVRLRQREFDLLEAFLRHPNVVLDRERLLSMVWGQEY
                     EGDTRTIDVHVAWLREKLRQSAVKIETVWGVGYKLVPSGDAG"
     gene            426..1949
                     /locus_tag="PLX85_05060"
     CDS             426..1949
                     /locus_tag="PLX85_05060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC62574.1"
                     /translation="MRVSLPPIGFRPSLRLRVLASFALVIALSLFLAGVVSVWLIRNQ
                     QAESAEKGIGRLVDPLSNEMQRMQLSGYPPERVAQELANLARYFDVRILLVDANQQVI
                     LDTNSNKPAIGEQIAEAKQTHVDSGPGESFQSQRIRLQGEDLFLFTASPRGDMPAQVE
                     RPASNVSLVVTVPATDVTSAWATLLPRLLIAGGLAAVFAAVVGSLLANRITRPIHEMT
                     RASEAMALGDYSQRIDVGDEDDELAHLGRAFNQMAQQVDRSTRTMRQLLADVSHELKT
                     PLTSIQGYSQAMVDGVLESDELPGAAEVVHQEAQRMRALVDDLLYLSQLETGQSSLTL
                     ERIDLDAAAAATANRFRYQAEAAGVSIGLDLGGGAIEGDERRIEQVLANLLDNAIRFA
                     PESSTVDVRTRRDGGWILVSVHNAGDPIPTEDLPNIFDRFYQVDPARTRNGHSGLGLA
                     IVRELVQAHGGEVRAESSAEAGTTFTVRLPAAVATRGAPMPAPRDEDVPPEAVPAGA"
     gene            2012..2272
                     /locus_tag="PLX85_05065"
     CDS             2012..2272
                     /locus_tag="PLX85_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRC62575.1"
                     /translation="MFDTILRAVVRVDAQGRLLVPKELRDAIDMRPNENVTLLVEDGE
                     LVVLTVTASRARLREIAAKYSTPGRSLVDELIAERRAEAERE"
     gene            2265..2654
                     /locus_tag="PLX85_05070"
     CDS             2265..2654
                     /locus_tag="PLX85_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017741932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRC62576.1"
                     /translation="MSDLVLDASAVLAFVKDETGADRVDELMRAGEAVVSAVNLAEAV
                     SKLTDDGWPIERVRSGIATTGARSVAFLVEDAYIAGLLRERTRHRGLSLGDRACLALA
                     LRLGAPALTADRSWGELELGIEVVLIR"
     gene            complement(2680..>3035)
                     /locus_tag="PLX85_05075"
     CDS             complement(2680..>3035)
                     /locus_tag="PLX85_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRC62577.1"
                     /translation="GLLGVIGYCFGGGYTWETAIASLDVKAAVPYYGTAPLDRIGRIR
                     AAVLAMYGEADARITSQAPEVERILRATGTTIEVKIYPGAGHAFFNDTGANYNAAAAA
                     DAWPRTLAWFRKYLA"
BASE COUNT          460 a         1022 c         1084 g          469 t
ORIGIN      
        1 caagatcgtc ggcctcgaga tgggggccga cgactatgtc accaagccgt tcaaccctcg
       61 cgaggtggtt gcgcgcgtca aggcggcact gcgtcgccgc gagtgggacc gcagcgatga
      121 tggccagcgt ggcgcagtct ccgtcggcga cgtcacgctc gacccctcga gccgcgacgt
      181 gaccgtgggt gagacgcgcg tgcggttgcg ccagcgcgag ttcgacctcc tggaggcgtt
      241 cctgcgccac ccgaacgtgg tgctggaccg cgagcggctg ctctcgatgg tgtggggcca
      301 ggagtacgag ggcgatacgc gcacgatcga cgtccacgtc gcctggctgc gcgagaagct
      361 gcggcagtcg gccgtgaaga tcgagacggt ctggggcgtg gggtacaaac tcgtccccag
      421 cggcgatgcg ggttagcctg cctccgatcg ggttccgccc cagcctgcgg ctgcgcgtgc
      481 tggccagctt cgcgctggtc atcgcgctga gcctcttcct cgccggcgtc gtctcggtct
      541 ggctgatccg caaccagcag gcggagtcgg cggagaaggg catcggccgc ctcgtcgacc
      601 cgctctccaa cgagatgcaa cgcatgcagc tcagcggcta cccgcccgag cgggtggcgc
      661 aggagctggc gaacctcgcg cgctacttcg acgtccgcat cctgctcgtc gatgccaacc
      721 agcaggtcat cctcgacacg aactcgaaca agcccgcgat cggcgaacag attgccgagg
      781 cgaagcagac gcacgtcgat agcgggcccg gagagtcctt ccagtcgcag cgtatccggc
      841 tgcaggggga ggacctcttc ctgttcacgg cgtctcctcg cggcgacatg cccgcgcagg
      901 tcgagcgccc ggcctcgaac gtgagcctgg tggtgacggt acccgccacc gacgtcacct
      961 cggcgtgggc gacgctgctg ccgcgtctgc tgatcgctgg cggcctggcc gcggtcttcg
     1021 cggcggtcgt tggctcattg ctcgccaacc gcatcacccg accgatccac gagatgacgc
     1081 gcgcctcgga agccatggcg ctgggcgact acagccagcg catcgacgtc ggcgacgagg
     1141 acgacgagct ggcgcacctc gggcgggcgt tcaatcagat ggcgcaacag gttgaccgca
     1201 gcacgcgtac gatgcggcag ctgctggccg acgtgtccca cgagctgaag acgccgctca
     1261 cctcgatcca ggggtactcg caggcgatgg tcgacggcgt gctcgagtcc gacgagctgc
     1321 ccggcgccgc cgaggtggtg caccaggaag cgcagcgcat gcgcgcgctg gtggatgacc
     1381 tgctctacct gtcccagctc gagaccggcc agtccagtct gaccctcgag cgcatcgacc
     1441 tcgacgccgc ggccgccgcg acggcgaacc gcttccgcta ccaggcggag gcggcgggcg
     1501 tgagtatcgg gctcgatctg ggcggcggcg cgatcgaggg cgacgagcgg cgcatcgagc
     1561 aggtgctcgc gaacctcctc gacaacgcga tccgcttcgc gcccgagtcc tcgacggtgg
     1621 atgtccgcac ccgccgcgac ggcggctgga tcctcgtcag cgtgcacaac gccggcgatc
     1681 cgatcccgac tgaggacctg ccgaacatct tcgaccgttt ctatcaggtg gacccggcgc
     1741 gcacgcgcaa cggccacagc ggcctgggcc tcgcgatcgt ccgcgaactg gtgcaggcgc
     1801 acggcggcga ggtgcgagct gagtcctcgg ctgaggccgg caccacgttc acggtgcgcc
     1861 tgccggccgc tgtcgcgacc cggggagcgc cgatgccagc gcctcgggat gaggatgttc
     1921 cgccggaggc ggtgcccgcg ggggcgtagc cccgccctcg atctggtagg attgtcgatc
     1981 tcaccagaag tgagagaagt ggcagcgttt catgttcgac accattctgc gcgcggtcgt
     2041 ccgcgtggac gcccagggcc ggctcctggt cccgaaggag ttgcgggacg ccatcgacat
     2101 gcggccgaat gagaacgtga ctctcctcgt cgaggacggc gagctcgtgg ttctgaccgt
     2161 gacggcgtca cgcgcccggc ttcgtgagat cgcggcgaaa tattcgacgc cgggccggtc
     2221 gctcgtggac gaactgatcg ccgaacggcg ggcagaagcc gagcgtgagt gacctcgtgc
     2281 tggatgcttc ggccgtcctg gccttcgtga aggacgagac cggtgccgac cgtgtcgacg
     2341 aactcatgcg agccggcgag gccgtcgtca gcgcggtcaa cctcgccgag gcggtctcga
     2401 agttgaccga tgatggctgg ccaatcgaac gcgtacgctc agggatcgcg accaccggcg
     2461 cgcgctccgt ggcctttctc gtcgaagacg cctacatcgc cgggctgctt cgggagcgta
     2521 cgcggcaccg cggactctcc ctcggcgacc gcgcctgcct ggcgctggcg ctgcgcctgg
     2581 gggcgccggc gctgacggcc gaccgctcat ggggagagct cgagctgggt atcgaggtgg
     2641 tgctgatccg atagagccgc gcggcgcgcc tcgatgcggc tacgcgaggt acttgcggaa
     2701 ccaggcgagg gtgcgcggcc acgcgtccgc cgctgcagcg gcgttgtagt tcgcgccggt
     2761 gtcgttgaag aaggcatggc ccgcgcccgg gtagatcttc acctcgatgg tcgtcccggt
     2821 ggcgcggagg atgcgctcca cctcgggggc ctgcgaggtg atgcgggcgt ccgcctctcc
     2881 gtacatggcg agcaccgcgg cgcgaatcct gccaatgcgg tcgaggggcg cggtgccgta
     2941 gtagggcacg gctgctttca catcgaggct agcgatcgcg gtctcccagg tgtagccgcc
     3001 gccgaagcag tagccgatga cacccaggag gccgg
//
LOCUS       DAORPG010000182         3033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3205_length_3033_cov_2.803224, whole genome shotgun sequence.
ACCESSION   DAORPG010000182 DAORPG010000000
VERSION     DAORPG010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3205_length_3033_cov_2.803224"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PLX85_05080"
     CDS             complement(<1..668)
                     /locus_tag="PLX85_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62578.1"
                     /translation="MLGGFTLAVALPVEAALGRFGTGWFAHAQVHGHLQVIGFAGLFV
                     VGVSTRLAPRFTNRTLHDPSIRVAFWLLASGLFLRALGQPLAQHAAFAAVMLIGGAFE
                     AAGALLALVTLTRTLRPWAAPRSASAALLNASAFWLTVQALLSLWWLRDLAVEGERIL
                     PANRNALLLEAQTFGFLLSALAGVGLRSFPTFFGAPPPPSRYAWGFAVVLQAGLVVWL
                     LGLGL"
     gene            complement(788..1318)
                     /locus_tag="PLX85_05085"
     CDS             complement(788..1318)
                     /locus_tag="PLX85_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRC62579.1"
                     /translation="MERSGPKQVLDALPVHPTPDLDVTRHMLRISGAVGQPSVLSPAD
                     LRSRPQVTLDRDFDCEEGWTVPGLTWTGVPLAALIEEAQPRAEAGWVTLASGDFATTV
                     ALDDVRDALVVLDLDDGPLPFEHGGPMRILVPGGACFTSIKWLDRIELTADPAEDSAR
                     HIALRRIGLEPDPSAA"
     gene            1449..1979
                     /locus_tag="PLX85_05090"
     CDS             1449..1979
                     /locus_tag="PLX85_05090"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRC62580.1"
                     /translation="MQIALGRKGDYSVRAMIDLARHYGDGRRKARQIARTMDIPERYL
                     PQLLAPLVRGGLLTATAGPDGGYALALPPTAISLLQVIELAEGPLESPECVLRGGPCD
                     WEGICPAHDAWVGGRAAVARHLEMTTLAELAERDAAIERGDVAPPPVALHPISVPRQG
                     LRDSQQVAPKRRARAR"
     gene            2070..>3033
                     /locus_tag="PLX85_05095"
     CDS             2070..>3033
                     /locus_tag="PLX85_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRC62581.1"
                     /translation="MTPNLGDYEQTAAAFDWDTARGWLDGLPGGGLNIAHEAVDRHAA
                     GARADHVAIRWRGRRGERRDISYGELGRLSNQFANVLASLGVAPGDRVFSLCPRVPEL
                     YVAALGTLKQRAVYCPLFPAFGPEPIRERLQRGGGVVLVTTESLYRRRVAPIRDQLPE
                     LRHVLVIAEPDQAPAGTLDFRASLAGASEEFAIGPTAPEDPALLHFTSGTTGRPKGAI
                     HVHEAVVAHHATARFALDLHPEDVFWCTADPGWVTGTSYGIIAPLTHGVTSIVEEAEF
                     DAAGWYQLLQDEGVTVWYTAPTAIRLLMRAGADLARQYDLGRLRL"
BASE COUNT          493 a          998 c         1141 g          401 t
ORIGIN      
        1 aggccgaggc ccaggagcca cacgacgagg ccagcctgga ggacgacagc gaatccccac
       61 gcgtaccgcg acgggggcgg cggcgcgccg aagaaagtcg ggaacgaacg cagcccaacg
      121 cctgcgaggg cggagagcag gaagccgaac gtctgcgcct cgaggagcag cgcgttgcgg
      181 tttgccggca ggatgcgctc accctcgacg gcgaggtcgc ggagccacca caggctgagc
      241 agcgcctgga cggtcagcca gaacgcactc gcgttgagca gagcggccga ggccgacctc
      301 ggcgctgccc acgggcgaag ggtgcgcgtc agcgtcacaa gcgcgaggag agcgccggcc
      361 gcctcgaacg ccccgccaat gagcatgact gcggcgaacg cggcgtgttg cgccagcggt
      421 tgtccaaggg cgcgcaggaa caggccggag gcgagcagcc agaacgcgac ccggatcgag
      481 gggtcgtgga gcgtgcggtt ggtgaaccga ggggcgagac gcgtcgagac tcctacgacg
      541 aacaggcctg cgaatccgat gacctgcagg tgcccgtgca cctgcgcgtg ggcgaaccag
      601 ccggtcccaa atcggccgag cgcggcctcc acggggaggg cgaccgcgag cgtgaagccg
      661 ccgagcacac caaacgcaag cgccgcgagg aggaagagga cgtgcgcctc ctcgtgaggc
      721 ggatgcgcgg gcggagcccc gggaggcggg ggtgcgttga aggtcgcgcc gaggccgcgc
      781 tcgcgggtca ggcggcgctc gggtcaggct cgaggccgat gcgccgcagc gcgatgtggc
      841 gtgcgctgtc ctcggcggga tcggcggtca gctcgatgcg gtcgagccac ttgatgctgg
      901 tgaaacaggc gccgccgggc accaggatgc gcatcggtcc tccgtgctcg aacggcaggg
      961 ggccatcgtc gaggtcgagg acgacgagcg cgtcgcggac gtcatcgagc gcgaccgtgg
     1021 tggcgaagtc gccggaggcc agggtgaccc aacctgcctc agcgcgcggt tgggcctcct
     1081 cgatcagggc tgccagcggg acgcccgtcc aggtgaggcc ggggacggtc cagccctcct
     1141 cacagtcgaa gtcgcgatcc agcgtgacct ggggacgaga gcggagatct gcgggagaca
     1201 ggacggaggg ctggccgaca gcgccgctaa tgcgcagcat gtgccgtgtc acatcgaggt
     1261 caggtgtggg atgaacgggc aacgcgtcga gaacctgctt tgggccggaa cgttccatgg
     1321 acgcgctccc ctcgaatgcc cgggcggact caggcgacac gcagtgcgcg gtgccgcgat
     1381 agctgtattt tcggccgtcc aggaagtatt gtctagctgc gcacttaacg attcaaaagg
     1441 gtctctgtat gcagattgcg ctggggcgaa aaggcgacta ctcggtgcgg gcgatgatcg
     1501 atctcgcccg tcattacggt gacggacggc gaaaagcgcg ccagatcgcg cggacgatgg
     1561 acattccaga gcgttacctg ccgcagctgc tggcccccct cgtgcggggc ggcctgttga
     1621 ccgcgacggc gggcccggac ggcggctacg cgctcgctct gccgccgacg gcaatcagcc
     1681 tgctgcaggt gattgagctg gcggaaggcc cgctcgagtc gcccgaatgt gtgctgcgag
     1741 gcgggccatg cgactgggag ggcatctgcc ccgcgcatga tgcctgggtc gggggccggg
     1801 ccgccgtcgc gcgtcacctt gaaatgacca cgctggcaga acttgcggag cgggacgccg
     1861 cgatcgaacg cggggacgtg gccccgccgc ccgtggcgct gcacccgatc agcgtgccga
     1921 ggcagggtct gcgggacagc cagcaggtgg ccccgaaacg gcgcgcgagg gcccgctgac
     1981 cattcgtcac ggcactcgcg ggacgttcgt cccgccgact tcgctgacgg gcatgccatc
     2041 ctcgtcgtga ggaggatcgc gtgagcccgg tgactccgaa cctcggcgac tacgagcaga
     2101 cagcggcggc gttcgactgg gacaccgcac gaggctggct cgacgggctg cccggcgggg
     2161 gcctcaacat cgcgcacgag gcggtcgacc gccatgccgc tggtgcgcgc gccgaccatg
     2221 tcgcgatccg gtggcggggc cggcgaggcg agcgacgcga catctcgtac ggcgagctgg
     2281 ggcggctgag caatcagttc gcgaacgtcc tcgccagcct cggggtggcg ccaggtgacc
     2341 gggtcttcag cctctgccca cgcgtgccgg agttgtacgt cgccgcgctc gggaccttga
     2401 agcagcgcgc cgtctactgc ccactcttcc ccgccttcgg cccggagccc atccgcgagc
     2461 ggctgcagcg aggcgggggc gtcgtcctcg tcacgaccga atcgctctac cggcggcggg
     2521 tagcgccgat ccgcgaccag cttcccgagc tgcggcacgt gctggtgatc gcggagccgg
     2581 atcaggcgcc ggccggcacg ctagactttc gcgcctcgct ggcaggagct tcggaggagt
     2641 tcgccatcgg gcccaccgcg cccgaagatc ccgcgctgct tcatttcacc agcgggacca
     2701 ccgggcggcc gaagggtgcg atccacgtcc acgaggcggt agtcgcgcac cacgcgaccg
     2761 cgcgattcgc cctcgacctg catccggagg acgtgttctg gtgcacggcc gaccccgggt
     2821 gggtcacggg aacctcgtac ggcatcatcg cgccgctgac tcacggcgtg accagcatcg
     2881 tcgaggaggc ggaattcgac gcggcggggt ggtaccagtt gctgcaggac gagggcgtga
     2941 cggtctggta cacggcgccc acagcgatcc ggctgctgat gcgggccggg gccgacctcg
     3001 cgcggcagta cgacctcggc cggctgcggc tgg
//
LOCUS       DAORPG010000183         3006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3265_length_3006_cov_3.910200, whole genome shotgun sequence.
ACCESSION   DAORPG010000183 DAORPG010000000
VERSION     DAORPG010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3265_length_3006_cov_3.910200"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..1288
                     /locus_tag="PLX85_05100"
     CDS             332..1288
                     /locus_tag="PLX85_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRC62582.1"
                     /translation="MEDPRIVADRILANVDRVIIGKALETRLALVCLMSRGHLLIEDV
                     PGVGKTMLARALARSAGCTFSRIQFTPDLLPSDVTGVSVFNQGTRDFEFRPGPIMAQI
                     VLTDEINRATPKTQSALLEAMEERQVTVDGVTHRTPDPFMVMATQNPIEYEGTFPLPE
                     AQLDRFLMRVHLGYPSATDEVLVMDAQQSGHPIESLDQVTSADEVLELQQAVRGIYID
                     PLIKQYIVTLVGATREHESVYLGASPRGSLALMRATQAYALLDGRDFVQPDDVKALAY
                     TTLGHRVIVSPGARVRGIDSKEVVEECLERVPVPGARARGSA"
     gene            1296..2555
                     /locus_tag="PLX85_05105"
     CDS             1296..2555
                     /locus_tag="PLX85_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRC62583.1"
                     /translation="MRRSIVWLRRYPNVAWALFVALACFVIGFATGFWLLFRLAYVIL
                     LVIPLVYLWTRSMINALEVEVHRYTQRVSQGQSLDGRIVLTSTSRIPKVWLEVRDEAN
                     VPGHMPHRVITLGARGSQSWHYETPTLRRGVYQLGPVVVTASDPFGFFRFTRTFGGTT
                     PVLVYPSAPELPNFYIPPANLPGDGRFRRRTHNITPNVAGLRAYEPGDSYNRIHWPAT
                     ARTGNLMVKLFELDPASDIWVVLDLERAVHVGEGDDGTEEAAVKICASIARYFLAANR
                     TVGFMSFGDDLRINEADRGANHYTRILESLAVARASGDVSLAKLLLEESRRFGRHTTV
                     VAITPSTASEWPLTLMGLQSRGVKVAAVLLEAETFGASASSLDVYGTLAAGGIYTYTV
                     KSSDDLGNVLAAGADVAALDPRSGGRR"
     gene            2552..>3006
                     /locus_tag="PLX85_05110"
     CDS             2552..>3006
                     /locus_tag="PLX85_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62584.1"
                     /translation="MTLRATWDRPAPPMTPAAGPGGRPRLPSFLSWEELLTFGLMLVA
                     LLSVVISVERADWVAEMPRLTVAALVGLVSGWILGRTRAPAYALHLVGVAIGLAVVVG
                     MVMHTLRLANPLVEHGVQARWSELWERIGIWFDQLWSGDVSTDPLPFVLL"
BASE COUNT          438 a         1007 c         1048 g          513 t
ORIGIN      
        1 gtgccgcccg aggactcggc gggcgcgtcg tcgccgccgc acccgatcag cgcggcgagc
       61 gggacaagtg cgactccccc cgctgcgagg cgcaggaagc gtcggcgatg cagcctcggt
      121 gagtgggacg gtggcgtggc gtcctcctgg agtgggcgct gattcgggac ggaggttcag
      181 gcgcgaatcg atggtacgcc gcgcgatggc aaggatccga gtgacccgca ccgagcgttg
      241 ttatactgaa ttgcctcctg ttgaggtgac tcccacatct cgaaatgacg cccgcgctgt
      301 ggagcacaga cgcgcacccc cgggagggcc cgtggaagac ccccgcatcg tcgccgaccg
      361 tattctggcc aacgtcgacc gggtgatcat cggcaaggcg ctcgagacgc ggctggccct
      421 cgtctgcctc atgtcgcggg gccacctgct gatcgaggat gtcccgggcg tgggtaagac
      481 catgctggcg cgcgcgctgg cgcgctcggc gggctgcacg ttctcgcgca tccagttcac
      541 gccggacctg ctgccctcgg acgtcaccgg cgtctccgtc ttcaaccagg ggacgcgtga
      601 cttcgagttc cggccggggc cgatcatggc tcagatcgtg ctaaccgatg agatcaaccg
      661 cgccacgcca aagacgcagt cggcgctcct cgaggcgatg gaggagcggc aggtcacggt
      721 ggacggtgtg acgcaccgga cgccggaccc gttcatggtc atggcgacgc agaacccgat
      781 cgagtacgaa ggcacgttcc cgctgcccga ggcgcagctc gaccgcttcc tgatgcgcgt
      841 gcacctcggt tacccctcgg cgaccgacga ggtgttggtg atggacgcgc agcagagcgg
      901 gcacccgatc gagtcgctcg accaggtgac ctcggctgac gaggtgctcg agttacagca
      961 ggcggtgcgg ggcatctaca tcgacccgct gatcaagcag tacatcgtga cgctggtggg
     1021 tgcgacgcgg gagcacgaga gcgtgtacct cggggcgtcg ccgcgtgggt cgctggcgtt
     1081 gatgcgcgcg acgcaggcct atgcgcttct cgatggtcgc gacttcgtgc aacccgacga
     1141 tgtgaaggcg ctggcgtaca ccacgctggg ccaccgcgtg attgtcagcc cgggcgcccg
     1201 agtccgcggg atcgatagca aggaagtggt cgaagagtgc ctcgagcgtg tgcccgtgcc
     1261 cggggcgcgc gcccgcggca gtgcctgagc ggcccatgcg acgctcgatc gtctggctgc
     1321 ggcgctatcc aaacgttgcc tgggcgctgt tcgtggcgct cgcgtgcttc gtgatcgggt
     1381 tcgccaccgg gttctggctg ctgttccgcc tcgcctacgt gatcctgctg gtcatcccgc
     1441 tcgtgtacct gtggacgcgt tcgatgatca acgcgctcga ggtcgaggtg caccgctaca
     1501 cccagcgggt gtcgcagggt cagtcgctcg acggacgcat cgtccttacc tcgacgtctc
     1561 gcatcccgaa ggtgtggctc gaggtgcgcg acgaggcgaa cgtgccgggt cacatgcccc
     1621 accgcgtcat cacactgggg gctcgcggtt cgcaatcgtg gcactacgag acgcccacgt
     1681 tgcgtcgagg ggtgtaccag ctcggcccgg tcgtcgtgac cgcgagcgac ccgtttggct
     1741 tcttccgctt cacgcggacg ttcggcggga cgacgcccgt cctcgtctat cccagcgcgc
     1801 cggagctgcc caacttctat atcccgccgg ccaacctgcc aggcgacggc cgcttccggc
     1861 gccggacgca caacatcacc ccgaacgtcg ccggcttgcg ggcatacgag ccgggtgact
     1921 cctacaaccg catccactgg ccggccaccg cgcgcaccgg gaacctgatg gtgaagctgt
     1981 tcgagctcga ccccgcctcg gacatctggg tcgtgctcga cctcgaacgc gcggtgcacg
     2041 tcggcgaggg tgacgacggc acggaggagg cagcggtcaa gatctgcgcc tcaattgccc
     2101 gctacttcct cgccgccaac cgcacggtcg gcttcatgtc gttcggcgat gacctccgga
     2161 tcaacgaggc cgaccgcggg gcgaaccatt acacgcgcat cctcgagtcg ctggccgtgg
     2221 cacgagcgag cggcgatgtg tccctcgcga agctgctgct cgaggagtcg cgacggttcg
     2281 ggcggcatac gacggtcgtc gccatcacgc cctccaccgc ctcggagtgg ccgctgacgc
     2341 tgatgggact gcagagccgc ggcgtgaagg tcgctgccgt gctgctcgaa gcggagacgt
     2401 tcggcgccag cgcgagttct ctcgacgtat acgggacgct cgcggccggt ggcatctaca
     2461 cgtacacagt gaagagctcg gacgacctcg gaaacgtgct cgcggcaggc gccgacgtcg
     2521 cggcgctcga cccgcgctcc ggaggccgtc gatgacgctg cgcgcgacgt gggaccggcc
     2581 ggctcctccg atgacgccgg ctgccgggcc gggcggccgc ccacgattgc cctcgttcct
     2641 gtcgtgggaa gagctgctga ccttcgggct gatgctcgtc gcgctgctgt ccgtggtgat
     2701 ctcggtcgag cgcgcggact gggtggcaga gatgccgcgg ctcaccgttg cggcgctggt
     2761 gggcctcgtg tcgggctgga tcctcggccg cacgcgtgcg cctgcctacg ccctacacct
     2821 cgttggcgtg gcaattggcc tcgcggtcgt cgtcggcatg gtgatgcaca cgctgcggtt
     2881 ggcgaatccg ctcgtcgagc acggcgtaca ggcgcggtgg tccgagttgt gggagcgcat
     2941 cggcatttgg ttcgaccagc tgtggtccgg cgatgtgtcg accgaccccc tgccgttcgt
     3001 gctcct
//
LOCUS       DAORPG010000184         3002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3277_length_3002_cov_3.215134, whole genome shotgun sequence.
ACCESSION   DAORPG010000184 DAORPG010000000
VERSION     DAORPG010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3277_length_3002_cov_3.215134"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..2350)
                     /locus_tag="PLX85_05115"
     CDS             complement(44..2350)
                     /locus_tag="PLX85_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008280775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRC62585.1"
                     /translation="MVATEPAKGFKVVGSRPVRPDGVDKVNGRAIYGADVRFPRMLFG
                     RVLRSPHAHAIVHRIDTSKAEALPGVTAVVTNADFPRQSDQELQVGEGEAASARWILD
                     NVLASEKVLYRGHAVAAVAATDPHIAEDALDLIEVEYEVLEPVIDVQRAMLESAPLLH
                     DSIVTEQVFGFFGMDGSEQAGRRPSNVSKKVEFTGGDLDAGFAAADVVLEREFDTPMY
                     HQGYIEPQNGTALWSEDDTINVWTSTQAYFGVRDQLAVLLKHPVSKIRVNPAEIGGGF
                     GGKSSVYMEPLAALLSKRSDHRPVKMSMTRQEVFEATGPTPGAHMRVKIGAKRDGTIT
                     AIDWEGIYESGAYPASAFTAGAMAAWGPYDIKNFRSTAWEVVNNKPRVQAYRAPAAPA
                     AAWSVESMVDEVARAIDLDPVELRLKNAMKEGGRRIDGAPHGPIGNVACLEAVRDSPH
                     YRSELSGEHRGRGIAAGFWFNIGGESGAFASIHPDGTVALNIGSADIGGSRASLSMQF
                     AEAMGIAYEQVKPQTVNTDSIGYTLPTVGSRTTFAGGWAVYETAQALRRKLEERAASI
                     WECDRSAVHYDDDAVVRGPGERSFTFNELAAQLQATGGLIQAEVDVAPTTSGPAFAAH
                     VVDVEVDPDTGKVQVLRYTAVQDVGRAIHPDYVEGQIQGGAAQGVGMALTEEYVFGED
                     GRMQNSTLLDYRMPTALDLPMIDVVMVEVPNPGHPYGVRGVGEVPIVPPLAAVTNAIR
                     DAIGVRATRLPASPDRLLDLIEEQRRQP"
     gene            complement(2472..2897)
                     /locus_tag="PLX85_05120"
     CDS             complement(2472..2897)
                     /locus_tag="PLX85_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HRC62586.1"
                     /translation="MTVPSEYYDASRQFEAFLVHARDEAGLTTTNQSFTMVEGVFRAF
                     RRRLNLDDAIRFADVLPPVARALFVADWDPEEPRRPFEDRAAMTREAQSLRGGHNFAP
                     DSCIRDVAAALRASVDEAEFDALLARLPEDARAFWAVET"
BASE COUNT          423 a         1056 c         1069 g          454 t
ORIGIN      
        1 cccctcagga cgctggccgc gcggcccgag gggtgacctg cgttcagggc tgccggcgtt
       61 gctcctcgat caggtcgagc aggcggtccg gcgaggcggg cagccgggtg gcccgcacgc
      121 cgatggcgtc gcggatcgcg ttcgtcaccg ccgcgagcgg cggcacgatc ggcacctcgc
      181 cgacgccgcg caccccgtac gggtggccgg ggttcggcac ctccaccatc acgacgtcga
      241 tcatcggcag gtcgagcgcg gtcggcatgc ggtagtcgag cagcgtcgag ttctgcatgc
      301 ggccatcctc accaaagacg tactcctcgg tgagcgccat gccgacgccc tgcgcggcgc
      361 cgccctggat ttgcccctcc acgtagtctg gatggatcgc gcggcccacg tcctgcaccg
      421 cggtgtagcg cagcacctgc accttgccgg tgtcggggtc gacctccacg tcgaccacgt
      481 gtgcggcgaa ggccgggccc gaggtcgttg gcgcgacatc gacctcggcc tggatcagac
      541 cgccggtcgc ctgcagttgg gccgcgagct cgttgaacgt gaacgaccgc tcgcccggcc
      601 cgcgtacgac cgcgtcgtcg tcgtagtgga ccgccgagcg gtcgcactcc cagatcgagg
      661 cggcgcgctc ctcgagcttg cggcgcagcg cctgcgccgt ctcgtagacg gcccagccgc
      721 cggcgaacgt cgtgcggctg ccgaccgtgg gcagcgtgta gccgatggag tcggtgttca
      781 ccgtctgggg cttcacctgc tcgtaggcga tgcccatcgc ctccgcgaac tgcatcgaga
      841 gcgaggcgcg cgagccgccg atgtccgccg agccgatgtt gagcgcgacg gtgccgtccg
      901 ggtggatcga ggcgaaggcc cccgactcgc cgccgatgtt gaaccagaag cccgccgcga
      961 tgccccggcc gcggtgctcg ccggacaact ccgagcggta gtgcggcgag tcgcgtacag
     1021 cctcgaggca ggcgacgttg ccgatggggc cgtgcggagc gccgtcgatg cggcggccgc
     1081 cctccttcat cgcgttcttc agccgtagct cgaccgggtc gaggtcgatg gcccgggcca
     1141 cctcgtccac catcgactcg acggaccacg ccgccgccgg cgccgccgga gcgcgatagg
     1201 cctggacgcg cggcttgttg ttcacgacct cccatgcggt ggagcggaag ttcttgatgt
     1261 cgtacggccc ccaggcggcc atcgcgccgg ccgtgaacgc cgaggccggg taggcgcccg
     1321 actcgtagat gccttcccag tcgatcgcgg tgatggtgcc gtcacgcttc gcgccgatct
     1381 tcacgcgcat gtgcgcgccc ggcgtcgggc cggtcgcctc gaagacctcc tggcgggtca
     1441 tcgacatctt cacggggcgg tggtcgctgc gcttcgagag cagcgcggcg agcggctcca
     1501 tgtacaccga ggacttgccg ccgaacccgc ccccgatctc cgcggggttc acccggatct
     1561 ttgagaccgg gtgcttgagc agcacggcga gctggtcgcg cacgccgaag taggcctgcg
     1621 tgctcgtcca gacgttgatc gtgtcgtcct cgctccagag cgcggtgccg ttctgcggct
     1681 cgatgtagcc ctggtggtac atcggcgtgt cgaactcgcg ctccaggacc acgtccgctg
     1741 ccgcgaagcc cgcgtcgagg tcgccgcccg tgaactcgac cttcttcgag acgttcgagg
     1801 gacgccgccc ggcctgctcg ctgccatcca tgccgaagaa gccgaatacc tgttcggtca
     1861 cgatggagtc gtgcagcagc ggagccgatt cgagcatcgc gcgctgcacg tcgatgaccg
     1921 gctccaggac ctcgtactcc acctcgatca ggtcgagcgc gtcctccgcg atatgcgggt
     1981 ccgtcgccgc gaccgcggcg acggcgtggc cccggtacag caccttctcc gacgccagca
     2041 cgttgtcgag aatccagcgc gcgctcgccg cctcgccctc gcccacctgc agttcctggt
     2101 cggactggcg cgggaagtcg gcattggtga cgacggcggt cacgcccggg agcgcttcgg
     2161 ccttcgaggt atcgatgcgg tgcacgatgg cgtgcgcgtg ggggctgcgg aggacgcgac
     2221 cgaagagcat gcgcgggaag cggacgtcgg cgccgtagat agcgcgcccg ttcaccttgt
     2281 cgacaccgtc cggacgcacc ggacgtgaac cgacgacctt gaatcccttc gcaggctcgg
     2341 tggcgaccac tcgcaggctc cttcctcggg acgcggcaat gcgaggcgcg cgcggggcga
     2401 cctcggacgg cgcggggacc gcgccatcac gtgcatcata gcgactggac gagggtgtgc
     2461 gggggcgcga gctacgtctc gaccgcccag aacgcgcggg cgtcttcggg caggcgtgcg
     2521 aggagcgcgt cgaactccgc ctcgtcgacg ctcgcgcgca gggcggccgc cacgtcgcgg
     2581 atgcagctat ccggcgcgaa gttgtgcccg ccacgcagcg actgcgcctc gcgagtcatc
     2641 gcggcacggt cctcgaaggg gcgtcgaggc tcctcgggat cccagtccgc gacgaagagc
     2701 gcgcgggcga cgggtgggag gacgtccgcg aaacgaatcg cgtcatcgag gttgaggcgg
     2761 cgccggaagg cgcgaaagac gccctcgacc atcgtgaatg actggttcgt cgtcgtcagg
     2821 cccgcctcgt cgcgggcgtg gacgaggaac gcctcgaact ggcgcgacgc atcgtagtac
     2881 tcggaaggga cggtcatgcc tcgagggtgc ccgttcagcg actcgaggcc agcgggtgcg
     2941 gcacgcggcg gcgcgctgca tcggtgaccg ctgaggccag ccgcagcggc gcccacacgc
     3001 cc
//
LOCUS       DAORPG010000185         3001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3281_length_3001_cov_3.580448, whole genome shotgun sequence.
ACCESSION   DAORPG010000185 DAORPG010000000
VERSION     DAORPG010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3281_length_3001_cov_3.580448"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /locus_tag="PLX85_05125"
     CDS             complement(<1..1589)
                     /locus_tag="PLX85_05125"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HRC62587.1"
                     /translation="MPSRVYEIASEYVDRAAALHPLGATHMGVPGHDHEMTDYSPQGE
                     DERAAFEREMLAALEAAPVEDERDRVAREAMSERLRLHAEMHEAGEQYQSLNILFSPL
                     QDVRQCFDLMPRDTEEQWRNIASRLRLVPFGLQSYRRTLEEGVRLGLRSTRRQTEGCA
                     EQSAVWGGRHEGTPPFFDSLVEGYDASPVGNEALRRELEDGARRAADAYAEMGRFLAE
                     EYLPSAESRDAVGPERYARSARSYNGIELDLDETYRWGWEELHRIEAEVLATCERILP
                     GGSVDEVQRLLESDPARAIEGVEAFQRWMQDLHERTIAELDGVHFDIPQPVKRIEAMI
                     APAGGALAMYYTGPSEDFSRPGRTWYPTGGKTRFPLWGEVSIAYHEGVPGHHLQIGHV
                     THLSDRLSRWQRLLGTTSGYAEGWALYAERLMADLGYLENPDYYLGMLRGQALRAARV
                     VVDIGLHLERSIPADERYHPGEPWTPELAHEFLLRRTYFPANFLASEVTRYLGMPGQA
                     ISYKVGERAWLAFASIRPYGLV"
     gene            1691..2182
                     /locus_tag="PLX85_05130"
     CDS             1691..2182
                     /locus_tag="PLX85_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRC62588.1"
                     /translation="MDADVRKTVLRMIPYGLYVLTADSGSDVGAGTINWVSQMSFEPP
                     LLALGVKKDSKTFAQIKAGGKLGLSFLGSGQGGLAFAFFGDTPIEDGQFVAKETRIPF
                     SRTPGGAIVLDDAHGWAELRLVDTVETGDHAVVVAEVTDVGQQVAEPQVLTLKELNLN
                     YGG"
     gene            complement(2179..2556)
                     /locus_tag="PLX85_05135"
     CDS             complement(2179..2556)
                     /locus_tag="PLX85_05135"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HRC62589.1"
                     /translation="MGRALVDAQYCYLTTRGRVSGNPHTIEIWFALQGDTLYMMAGDG
                     ARADWVRNLVRTPEVSVRIEDRHFRGQARVVEAASPEDALARRLLVAKYRPGYAGDLS
                     DWERNGLPVAVSLDETEASVGGG"
     gene            2741..>3001
                     /locus_tag="PLX85_05140"
     CDS             2741..>3001
                     /locus_tag="PLX85_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62590.1"
                     /translation="MRRAVRLRVAVMAGAFVAALLPAALLAQTPTQTSTATATATATQ
                     TATATATQTATMTATATQTATATATATRTATATTTATSTATAT"
BASE COUNT          468 a          992 c         1083 g          458 t
ORIGIN      
        1 acgaggccgt acgggcggat actcgcgaac gcgagccagg cgcgctcgcc gactttgtag
       61 ctgatggcct gcccgggcat gccgaggtag cgggtgacct cggaggccag gaagttggcg
      121 ggaaagtagg tgcgccggag caagaactcg tgcgccaact cgggggtcca cggttcaccg
      181 gggtggtagc gctcgtctgc cgggatcgac cgctcgaggt ggagaccgat gtcgaccacg
      241 acccgagccg cccgcagggc ctgcccgcgc aacatgccga ggtagtagtc ggggttctcg
      301 aggtagccga ggtcggccat gagacgctcg gcgtacaacg cccagccctc ggcgtagccg
      361 gacgtcgtgc cgaggagacg ctgccacctc gagaggcggt ctgagaggtg ggtgacgtgg
      421 ccgatctgga ggtgatggcc ggggacgcct tcgtggtagg cgatggagac ttcgccccag
      481 agggggaagc gtgtcttgcc gcccgtgggg taccaggtgc gtccggggcg gctgaagtcc
      541 tcggatggcc cggtgtagta catggcgagc gcgccgccgg cgggggcgat catcgcctcg
      601 atgcgtttca ccggctgcgg gatgtcgaaa tggacgccgt ccagctcggc gatcgtccgc
      661 tcgtggagat cctgcatcca gcgctggaag gcctcgacgc cctcaatggc gcgagcgggg
      721 tcggactcga ggagccgctg cacctcgtcg actgagccgc cgggcaggat gcgctcgcag
      781 gtcgcgagga cctcggcctc gatgcggtgc agctcctccc agccccaacg ataggtctca
      841 tcaaggtcca gctcgatgcc gttgtagctg cgagcggaac gcgcgtagcg ctccgggccg
      901 acggcgtcgc gagattccgc cgaaggcagg tattcctcgg cgaggaatcg ccccatctcg
      961 gcgtaggcgt ccgccgcgcg cctcgcgccg tcctccaact cgcgccgcag cgcctcgtta
     1021 ccgaccggcg aagcgtcgta tccctcgacc agtgagtcga agaagggcgg cgtgccctcg
     1081 tggcgtccgc cccagaccgc cgactgctcc gcgcaaccct ccgtctggcg gcgtgtcgag
     1141 cgcaggccga ggcggacgcc ttcctcgagc gtgcgccggt agctctgcag cccgaagggg
     1201 acgaggcgga ggcgagaggc gatgttgcgc cactgctcct cggtgtcccg gggcatgagg
     1261 tcgaagcact gccgcacgtc ctgcagcggg ctgaagagga tgttcagcga ctggtactgt
     1321 tcgccggcct cgtgcatctc ggcgtgcaga cgcagccgct cggacatcgc ttcccgagcg
     1381 acgcggtccc gttcgtcctc gacgggcgcg gcctcgaggg cggcgagcat ctcgcgttcg
     1441 aaggcggcgc gttcgtcctc gccctgtggc gagtagtcgg tcatctcgtg gtcgtgaccg
     1501 ggcacgccca tgtgcgtggc gccaagcgga tgcagcgccg cggcgcggtc gacgtactcg
     1561 ctggcgatct catagacgcg tgacggcatc agggcgcctt ccgtggtgcg ctggcaggac
     1621 tcgacatcct cgatggtaca ggcgccatcg gagccgccgt atactgcgct cccgacgaga
     1681 gggggacgcg atggacgcgg acgtacgcaa gacggtgctt cggatgatcc cgtacggcct
     1741 gtacgtgctg acggccgaca gcggatcgga cgtgggcgcg gggacgatca actgggtgtc
     1801 gcagatgtcg ttcgagccgc cgctactcgc gctcggcgtg aagaaggaca gcaagacgtt
     1861 cgcgcagatc aaggcgggcg ggaagctggg gctgtcattc ctcggcagcg ggcagggcgg
     1921 gcttgcgttc gcgttcttcg gcgacacgcc gatcgaggac gggcagttcg tggcgaagga
     1981 gacccgtatc ccgttctcgc ggacaccggg cggcgcgatc gtcctcgatg acgcgcacgg
     2041 ctgggcggag ctgcggctgg tggacacggt ggagaccggc gaccacgccg tggtcgttgc
     2101 cgaggtgacc gacgtgggac agcaagtggc ggagccgcag gtccttaccc tgaaggagct
     2161 gaacctcaac tacggcggct agccgccgcc gacggaggcc tcggtctcat cgagactcac
     2221 cgccacgggc aatccgttgc gttcccagtc cgagaggtcc cccgcgtagc ccggccggta
     2281 cttcgcgaca agcagccggc gcgcgagcgc gtcctccggc gaggcggcct cgacgacgcg
     2341 ggcctggccg cggaagtgac gatcctcgat gcgcacgctc acctcgggcg tccgcacgag
     2401 attgcgcacc cagtcagcgc gggcgccgtc gccggccatc atgtacagcg tgtcgccctg
     2461 gagggcgaac cagatctcga tggtgtgcgg attcccgctg acgcggccgc gcgtggtgag
     2521 gtagcagtac tgtgcgtcga ccagtgcccg acccatatgc gttctccgtt cggcggtgat
     2581 agcgagcagg ctaccgcgat cgttctccgt ccgtcgtttc gttagctctg gccccatttc
     2641 gttacgcaat tgatcctcgg gctcgcccag catggtcgct cggcccgcgg cagctcgctg
     2701 ccgggtgatt cagcgcgatc aggaggattc gatcatgagg atgcgacgag cagtgagatt
     2761 gcgagtggcc gtcatggccg gtgcgttcgt agcggcgctg ctgccggcgg ccctgctggc
     2821 gcagacgccg acacagacct cgacggcgac cgccaccgcg accgccacgc agacggcgac
     2881 cgcaacagcg acgcagaccg cgacgatgac cgccaccgca acgcagacgg caacggcgac
     2941 cgccaccgcg acccgcaccg cgaccgcgac gaccaccgcc acctcgacgg cgactgccac
     3001 g
//
LOCUS       DAORPG010000186         2999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3286_length_2999_cov_4.146399, whole genome shotgun sequence.
ACCESSION   DAORPG010000186 DAORPG010000000
VERSION     DAORPG010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3286_length_2999_cov_4.146399"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2134)
                     /gene="carB"
                     /locus_tag="PLX85_05145"
     CDS             complement(<1..2134)
                     /gene="carB"
                     /locus_tag="PLX85_05145"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HRC62591.1"
                     /translation="MRKVLIIGSGPIVIGQAAEFDYAGTQACKAMREEGIVSVLVNSN
                     PATIMTDEDVADIIYIEPLSVEVLERIIERERPDGLLPTLGGQTGLNLAVQLAEAGIL
                     ERYGVRMLGTPLRTIRLAEDRELFRQLLTDIGEPTPDSEIVESWEQAVAARERLGLPL
                     VVRPAYTLGGTGGGIAHDDEEYEQIVRGGLAASPITQVLVERSLLGWKELEYEVMRDG
                     ADTCITICNMENMDPMGVHTGDSIVVAPSQTLSDRDHQMLRSSALKIIRALGIEGGCN
                     VQFALAPRPDIVPWAGSDDATVPPYYVIEVNPRVSRSSALASKATGYPIARMAAKIAI
                     GKRLDEIKNPVTQQTSAAFEPALDYCVVKIPRWPFDKFAFGDRRLGTQMKATGEVMAV
                     DRSFEAALQKAVRSLEISNRSVLYQDPRWSERDMLEEPIEATDERLWKLLAALRQGVT
                     PLVLSRRTGIDPWFTERLARIVQMEQRLLSEELTPSLLVAAKRMGFGDAQIGTLADRL
                     PEQVRQMREQLGLRPVYKMVDTCAAEFEAATPYFYSTYEEENDAVPDHHERVLVIGSG
                     PIRIGQGIEFDYASVQAAHALQQAGVRAILANSNPETVSTDFDTSDRLYFEPLDEESV
                     RDLLENEAGGGEAPASIVQFGGQTAVNMAGVLQRSALPIAGSSADSIDLAEDRGRFEG
                     IMSALNIPQPPGSAVQTLAEALPTAEAVG"
     gene            complement(2158..2916)
                     /locus_tag="PLX85_05150"
     CDS             complement(2158..2916)
                     /locus_tag="PLX85_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62592.1"
                     /translation="MTSNTTTDAAAEYAARRAEDWAAFTAKSGPGADRWAAWAELSYQ
                     WKNAVEFLQPVGLDYPSYLEPLQPLLERLGTMEEVDLPPAEFTHLTVLRLGFLMSSDI
                     LWSQTESFYVNAAPRIHRVQPFSIRIGGISAHEDVLYLGVDDGHQFREVRRQIRLGVM
                     KAAEMLKSDPEWSPEADRYMPIVPFAYFTGRGDRARVIEAVEPYLNTELGEHPITKIK
                     MGRVASDPDIHYPGMDVIAEIGLLGQEYRKGYHN"
BASE COUNT          450 a         1030 c          995 g          524 t
ORIGIN      
        1 agccgacagc ctcggccgtc ggcagcgcct cagcgagcgt ctgcactgcc gagcccggcg
       61 gctgcgggat gttcagtgcg gacataatgc cctcgaagcg cccgcggtcc tccgcgaggt
      121 cgatcgagtc tgccgaggag cccgcgatcg gcagcgccga acgctgcagc acgccggcca
      181 tgttcacggc cgtctgcccc ccgaactgca cgatcgaggc cggcgcctca ccgccgcccg
      241 cctcgttctc gagcaggtcg cgcaccgact cctcgtcgag gggctcgaag tacaggcggt
      301 cggaggtgtc gaaatccgtg gatacggtct ccggattcga gttcgcgagg atggcacgca
      361 cgcccgcctg ctgcagcgca tgagcagcct gcaccgaggc gtagtcgaat tcgatgccct
      421 ggccgatgcg gatcggcccc gacccgatca cgagcacacg ctcgtggtgg tcggggaccg
      481 cgtcgttctc ttcctcgtac gtgctgtaga agtacggcgt cgccgcctcg aactccgcgg
      541 cgcaagtgtc gaccatcttg tacacgggcc gcagaccgag ttgttcgcgc atctggcgca
      601 cctgctcggg caggcggtca gcgagcgtgc cgatctgcgc gtcgccgaac cccatccgtt
      661 tcgcggcgac cagcagggac ggcgtcagct cctcggacag cagccgctgc tccatctgca
      721 cgatgcgagc gagccgctcc gtgaaccacg ggtcgatgcc cgtgcgtcgc gacagcacga
      781 ggggcgtcac gccctggcgc agcgcggcga ggagcttcca tagccgttcg tccgtcgcct
      841 cgatcggttc ttcgagcatg tcccgttcag accagcgtgg gtcctggtac agcaccgagc
      901 ggttgctgat ctcgagggag cgcacggctt tctggagcgc cgcctcgaag ctacgatcga
      961 ccgccatcac ctcgccggtc gccttcatct gcgtcccgag acggcggtcg ccgaacgcga
     1021 acttgtcgaa tggccagcgc ggaatcttca cgacgcagta gtcgagtgcc ggctcgaagg
     1081 ccgcgctcgt ctgctgcgtg accggattct tgatctcgtc cagccgcttg ccgatggcga
     1141 tcttggccgc catgcgtgcg atcgggtagc ccgtcgcctt cgaggccaac gccgaggaac
     1201 gcgacacgcg cggattcacc tcgatcacgt agtacggcgg cacggtcgcg tcgtccgacc
     1261 cggcccaggg cacgatgtcc ggccgcggcg cgagcgcgaa ctggacgttg cagccgccct
     1321 cgatgcccag tgcgcggatg atcttcagcg cgctgctgcg cagcatctgg tggtcccgat
     1381 ccgatagcgt ctgcgagggc gccacgacga tcgagtcgcc cgtgtgtacc cccatcgggt
     1441 ccatgttctc catgttgcag atggtgatgc aggtgtccgc gccgtcgcgc attacctcgt
     1501 attcgagttc tttccacccg agaagcgatc gctccaccag cacctgcgtg atcggcgacg
     1561 ccgcgaggcc gccccgcacg atctgctcgt actcctcgtc gtcatgggcg atgccaccgc
     1621 cggtgccgcc gagggtgtat gccggccgca cgaccagggg cagcccgagt cgctcccgcg
     1681 cggcgaccgc ctgctcccag ctttcgacga tctcgctgtc cggtgtcggt tcgccaatat
     1741 cggtcaacag ctggcggaac agctcgcggt cctctgcgag gcggatcgtg cgcagcggcg
     1801 tgcccagcat gcgcacgccg taccgctcga ggatgccggc ctccgcgagc tgcaccgcga
     1861 ggttgaggcc cgtctggccg ccgagcgtcg gcaacagtcc gtccggccgc tcccgttcga
     1921 tgatccgttc gaggacttcc acggacagcg gctcgatata gatgatgtcg gcgacgtcct
     1981 cgtctgtcat gatcgtggcc gggttcgagt tcacgaggac ggagacgatg ccttcctcgc
     2041 gcatcgcctt gcacgcctgc gtaccggcgt agtcgaactc cgccgcctgg ccgatgacaa
     2101 tcgggccgct gccgatgatg aggactttgc gcagcggcgc ctcggggtct ggtgtcgtca
     2161 gttgtggtac cccttgcggt actcctggcc cagcaggccg atttccgcga tcacgtccat
     2221 gcccgggtag tggatgtccg ggtcgctggc aacgcggccc atcttgatct tcgtgatcgg
     2281 gtgctcgccg agctcggtgt tcaggtatgg ctcgacagcc tcgatgaccc gcgcacggtc
     2341 gccgcgcccg gtgaagtagg cgaacggcac gattggcatg tagcggtccg cctccggcga
     2401 ccactccggg tcggacttca gcatctccgc cgccttcatg acgccgaggc ggatctgccg
     2461 ccgcacctcg cggaactggt ggccgtcgtc cacaccgaga tacagcacgt cctcgtgggc
     2521 ggagatgccc ccgatgcgga tcgaaaacgg ctgcacgcga tggatgcgtg gcgcggcgtt
     2581 cacgtagaac gactccgtct gcgaccacag gatgtccgag gacatcagga accccagccg
     2641 cagcacggtc aggtgcgtga actccgcggg cggcaggtcg acttcctcca tcgtccccag
     2701 gcgctcgagc agcggctgga ggggttcgag gtaggacggg tagtcgagtc ctacgggctg
     2761 caggaactcg accgcgttct tccactggta tgacagttcc gcccaggcgg cccagcggtc
     2821 ggctcccggt ccggacttcg cggtaaacgc tgcccagtcc tcggcgcggc gcgccgcgta
     2881 ctctgcggcg gcgtcggtgg tggtgttact cgtcacggcg cacctcgtag atgggcatgt
     2941 cgtactgcgc gcccgggttg atgatcggga ccagcgcgcc aatcgtggag aggaaggac
//
LOCUS       DAORPG010000187         2999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3290_length_2999_cov_2.844429, whole genome shotgun sequence.
ACCESSION   DAORPG010000187 DAORPG010000000
VERSION     DAORPG010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3290_length_2999_cov_2.844429"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PLX85_05155"
     CDS             <1..411
                     /locus_tag="PLX85_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC62593.1"
                     /translation="TLAGAIAAHLRVCSSEEQDVVEELFSELFGADHSWVPRLLRGPA
                     DVLTERLEDGLADVLSFGIHDWSAEAFGGAAHCWKPGVDSTQVRARLRAFALEGSSVA
                     NVHVCGEAYSDFQGFIEGSLRSANHAVESVLGAG"
     gene            438..665
                     /locus_tag="PLX85_05160"
     CDS             438..665
                     /locus_tag="PLX85_05160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HRC62594.1"
                     /translation="MFNIRAESDLAIHPGELLAEELEARGLTQRQLALNIDRTVQAIN
                     EIVRGKKAITAETALQLEEALGVPAHLWVNL"
     gene            797..2014
                     /locus_tag="PLX85_05165"
     CDS             797..2014
                     /locus_tag="PLX85_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC62595.1"
                     /translation="MDWNDTPEQAAFRADARAFIEASLPKYYHDQIVNGPEDDNPEAG
                     WQFDMMHGTDSAKAAAHQWMEALNSRGWAAPSFPSEYGGAGLSTIEQFILKQELARVE
                     APEVGGAHLMQIGPTLLVHGTEEQKKRFLPPTLKGEYLWAQGFSEPGAGSDLASLQCR
                     AVREGDEYVVNGQKLWTSAGHMANWIFMLTRTDPDAPKHRGITFLMADMTTPGISVRP
                     IISMGWHHATNETFYEDVRIPVDQVVGEVNRGWYVGMTLLDFERSGIAGAMQQQRALN
                     ALIEDVKGGQRPNRAHQLRHEIADRFIEVDINSNLSLRIASMQSRGEIPNYEASMGKA
                     YSSELSQRVSRTGTKVYGLYGNLWSSKEPLAPLRATHTQTYVSSVVITIAGGSSEIQR
                     NIIATRGLGLPRG"
     gene            2253..>2999
                     /locus_tag="PLX85_05170"
     CDS             2253..>2999
                     /locus_tag="PLX85_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HRC62596.1"
                     /translation="MTARAAPVRPTGFDPRWLPLAVTTVGSFMSVLDTSIVNIALPSI
                     LKDFGASIEEGQLVITSYLMALAVVIPASGFLAERVGMKRLYIITLISFTVGSALCGM
                     AWNVQSLTFFRVLQGLGGGMIQPLGMAIVFTLITPLERPYFMGLLGIPVLLGPVLGPS
                     LGGYLTEYASWRAIFLINVPIGLIDLGLAIWLLKETPIRHTAKLDTRGFVLSAITFPS
                     LVLALSWGAQHGWGSPLVLALLAVGLVGLTL"
BASE COUNT          457 a          863 c         1195 g          484 t
ORIGIN      
        1 acgctcgcag gggcgatcgc ggcgcacctg cgggtgtgct cctcggagga gcaggacgtg
       61 gtcgaggagc tgttctcgga gctgttcggg gcggaccaca gctgggtgcc gcggctgctg
      121 cgcgggccgg cggacgtgct gacggaacgc ctcgaggacg ggctggcgga cgtgctgtcg
      181 ttcgggatcc acgattggtc cgcagaggcg ttcggcgggg cggcgcactg ctggaagccg
      241 ggggtggact cgacgcaggt gcgggcacgg ctgcgtgcgt tcgccctcga agggtcgtcg
      301 gtggcgaacg tgcacgtgtg cggggaggcg tactcggact tccaggggtt catcgagggg
      361 tcgctgcggt cggcgaacca cgcggtggag tctgtgctgg gggcggggta ggggcgggag
      421 tcggctacga tgggggcatg ttcaacatcc gcgccgagtc cgacctcgcg attcacccgg
      481 gcgagctgct cgccgaggag ctcgaggctc gagggctgac gcagcgccag ttggcgctca
      541 acatcgaccg gactgtgcag gcgatcaatg agatcgtgcg gggaaagaag gcgatcacgg
      601 cggagacggc gctccagctc gaagaggcgc tcggcgtccc ggcgcacctg tgggtgaacc
      661 tgtaggcggc ctacgacgtg tcgtgcgcgc ggcggaggcg ggcggcctcg cgtggcggtt
      721 gactcctcgt tgggtcgggc gtatcagcta ggtggaacct tcgattagta cggacctctg
      781 aggagggata gacgcgatgg actggaacga cacaccggag caggcggcgt tccgcgcgga
      841 tgcgcgggcg ttcatcgagg cttcgctgcc gaagtactac cacgaccaga tcgtgaacgg
      901 gccggaggat gacaatccgg aggccgggtg gcagttcgac atgatgcacg ggacggactc
      961 ggcgaaggcg gcggcccatc agtggatgga ggcgctgaac tcgcgcgggt gggcggcgcc
     1021 gagcttcccg tcggagtacg gcggggcagg gctgtcgacg atcgagcagt tcatcctcaa
     1081 gcaggagctg gcgcgggtgg aggcgcccga ggtgggcggc gcgcacctga tgcagatcgg
     1141 gccgacgctc ctcgtgcacg ggaccgagga gcagaagaag cggttcctgc cgccgacgct
     1201 caagggggag tacctgtggg cgcaggggtt ctcggagccg ggggcggggt ctgacctcgc
     1261 gtcgctgcag tgtcgcgcgg tgcgcgaggg cgacgagtac gtggtgaacg gtcagaagct
     1321 gtggacctcc gcgggtcaca tggcgaactg gatcttcatg ctgacgcgga cggacccgga
     1381 tgcgccgaag catcgcggga tcacgttctt gatggcggac atgacgaccc cgggcatctc
     1441 ggtgcggccg atcatctcga tggggtggca ccacgcgacg aacgagacgt tctacgagga
     1501 cgtgcggatc ccggtcgacc aggtcgtggg cgaggtgaac cgcggctggt acgtggggat
     1561 gacgctgctg gacttcgagc ggtcgggcat cgcgggcgcg atgcagcagc agcgggcgct
     1621 gaacgcgctg atcgaggacg tgaagggcgg gcagcggccg aaccgagcgc accagctgcg
     1681 gcacgagatc gccgaccgct tcatcgaggt ggacatcaac agcaacctgt cgctgcggat
     1741 cgcctcgatg cagtcgcggg gtgaaatccc gaactacgag gcgtcgatgg ggaaggcgta
     1801 ctcctcggag ctgtcgcagc gggtgtcgcg gacggggacg aaggtgtacg ggctgtacgg
     1861 gaacctgtgg tcctcgaaag agccgctggc gccgctgcgg gcgacgcaca cgcagacgta
     1921 cgtgagcagc gtggtgatca cgatcgcggg cgggtcgagc gagatccagc ggaacatcat
     1981 cgcgacgcgg gggctggggc tgcctcgagg gtagtccccg ccggtcaggg ttcattcgcg
     2041 aagcgccgcc ggggcatggt tccggcggcg cttcttcgtg tcgcgagccg ccgccacccc
     2101 gggtctggac ggtacttggc gaggggcagc gccccttagc agacctgcat gattaaatag
     2161 cgctacatgg tcagggagac actgatgttg tcgcgggcgc ggcgcattcc tcgtggtgag
     2221 gcgcgcggac cgcggcggcg gggaggcgga cgatgacggc gcgggcggct ccggtgcggc
     2281 cgacgggctt cgatcctcgg tggctgccgc tggcggtgac gaccgtgggg tcgttcatgt
     2341 cggtgctgga cacctcgatc gtcaacatcg cgctgccgag catcctgaag gacttcgggg
     2401 cgagcatcga agagggtcag ctcgtgatca cgagctacct gatggcgctc gcggtggtga
     2461 tcccggcgag cgggttcctg gccgagcggg tcgggatgaa gcggctgtac atcatcacgt
     2521 tgatctcgtt cacggtcggc tcggcgctgt gcgggatggc gtggaacgtg cagagcctga
     2581 cgttcttccg cgtcctgcag gggctcggcg gcgggatgat ccagccgctg ggcatggcga
     2641 tcgtcttcac gctgatcacg ccgctggagc ggccgtactt catggggctg ctcgggatcc
     2701 cggtgctcct cggtccggtg ctcgggccgt cgctcggcgg gtacctgacg gagtacgcga
     2761 gctggcgcgc gatcttcctg atcaacgtgc cgatcgggct gatcgacctc gggctcgcga
     2821 tctggctgct gaaggagacg ccgatccggc acacggcgaa gctcgacacg cggggctttg
     2881 tgctgtcggc gatcacgttc ccgagcctcg tgctagcgct gagctggggc gcacagcacg
     2941 ggtggggatc gccgctggtg ctggcgctgc tggccgtggg gctcgtgggg ctcacgctc
//
LOCUS       DAORPG010000188         2985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3318_length_2985_cov_3.819113, whole genome shotgun sequence.
ACCESSION   DAORPG010000188 DAORPG010000000
VERSION     DAORPG010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3318_length_2985_cov_3.819113"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..532
                     /gene="rpsO"
                     /locus_tag="PLX85_05175"
     CDS             266..532
                     /gene="rpsO"
                     /locus_tag="PLX85_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRC62597.1"
                     /translation="MQTTERKQQIITDYARFPGDTGSPEVQIALLTDRIRTLTDHLRV
                     HRHDFDTRRGLLMLVGQRRRQLRYLSNTDVDRYHTLLARLGLRR"
     gene            810..>2985
                     /locus_tag="PLX85_05180"
     CDS             810..>2985
                     /locus_tag="PLX85_05180"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRC62598.1"
                     /translation="MTFEVGTGLLAQNADASCTVRLGDTVLLATVCDGDPRPGIDFFP
                     LTVDFEERMYAIGKIPGSFFRREGRPGSEATLAARMTDRPIRPLFPKGYRREVQVVLT
                     LLSSDRENPADVFGTTAASIALGMSKIPFAGPVSSVRVARLDGEFKAFPTYDELEAAD
                     LDLIVAGTEHSVVMIEAGAKEVDEADVIEAIEYAMGVIAQLNGLQQEIVAALGVPKLE
                     YEAPAGIDPALQQQVHDILMSREQEMLDAVKMEGSHGIATLTTQVIEAMGGDEEPDRR
                     TVQSAIDAVIKPFVRKRILEQDRRTDDRSSQEIRELTAMVGLLPRAHGSGLFQRGQTQ
                     VLTAATLGPLADRQRLDNISPEQFKRYMHHYNFPPFSVGEARPLRGAGRREIGHGLLA
                     ERALEPGIPSAEDFPYTLRCVSDVLMSNGSSSMASVCGSTLALMDAGVPISAPVAGVA
                     MGLVTDEAGENFRVLTDIAGIEDAYGDMDFKVAGTAAGVTAIQLDIKLQRLPADLLET
                     VFRDAKAARERILAAMAQAIEEPREELSPYAPRTETIKIDREKIGTVIGPGGRVIRGM
                     VEETGATIDVEDDGTIHVGGSDPEGVRRAIEMIRGLTKEVMVGEQYEGPVTRIMTFGA
                     FVEILPGKEGLVHISELAEHRVNRVEDEVQIGDRVKVLVVEIDNLGRINLSRRALLEG
                     GDGRVGTQEGEEEDFFDEDEIPRRPAPGEAPRSVMSGTGAYGG"
BASE COUNT          493 a          978 c         1078 g          436 t
ORIGIN      
        1 aagggccagg gctgggtgac ggccgagacc aagggcgcgt gcctggtaag cggcggccac
       61 gtcgagggct gatcgcgccc gcatccttcg gggcgggccg cgcgcccgcc tcgccgtccc
      121 gtcgttgctc gatggttgtg ggctgtgcta gcgtgaacgc acgctcactc gacttgcggt
      181 gaccgcagcg gggcgtcgtg ccggtcggga cgaccggtgg cgagatgtgt ggcaggtagc
      241 agaacacgga ttcaaacggg caacgatgca gacgaccgaa cgcaagcagc agatcatcac
      301 ggactacgcc cggttccccg gggacacggg atcgcccgag gtgcagatcg cgctcctgac
      361 ggaccgcatc cgcacgctga cggatcatct gcgggtgcat cggcacgact tcgacacgcg
      421 acgcggcctg ctgatgctgg tgggccagcg caggcggcag ttgcgctacc tgagcaatac
      481 ggacgtcgac cgctaccaca cgctgctcgc ccgcctcggt ctgcgccggt aagtccggcg
      541 agccgacgca acgcagtctg ctccgggggc cggaggggct gctgctgacc aggatgcagc
      601 gaggaacccg tcgagacgac tcctcccgca ttacacgctg acgcgttgtt ccagacccgg
      661 gcacgggctg gcacctccac gccgcgcgtc aggctcggat gcaatggccg cgagacggcc
      721 cagggcacag aacagaccct tgaacggccc caaggaaggc atcgtcattg acaccccaac
      781 tacgaacgtt tagccgcgag atcgaaggga tgaccttcga ggtcggtacg ggcctcttgg
      841 cccagaacgc cgacgcttcc tgcacggtgc gcctcggtga caccgtgctc ctcgcgacgg
      901 tctgcgacgg cgacccgcgg cccggcatcg acttcttccc gctcaccgtc gacttcgagg
      961 agcggatgta cgccatcggg aagatcccgg gctcgttctt ccgccgcgag ggacggccgg
     1021 gctcggaggc gacgctggcg gcacgcatga cggaccgccc gatccgcccg ctattcccga
     1081 aggggtaccg ccgcgaggtg caggtcgtgc tgacgctgct gtcctcggat cgggagaacc
     1141 cggcggacgt gttcggtacc accgcggcct cgatcgccct tgggatgtcg aagatcccgt
     1201 tcgcggggcc ggtctcctcg gtgcgtgtcg cgcgccttga tggggagttc aaggcgttcc
     1261 cgacctacga cgagctggag gccgctgacc tcgacctgat cgtcgcgggc accgagcact
     1321 cggtcgtgat gatcgaggcg ggcgcgaaag aggtcgacga ggccgacgtc atcgaggcga
     1381 tcgagtacgc catgggggtc attgcgcagc tgaacgggct gcagcaggag atcgtcgcgg
     1441 ccctcggtgt gccgaagctc gagtacgagg cgcccgccgg catcgacccg gctctgcagc
     1501 agcaggtgca cgacatcctc atgtcgcgcg agcaggaaat gctcgacgcc gtgaagatgg
     1561 agggctcgca tggcatcgcc acgctgacga cgcaggtgat cgaggcgatg ggcggcgacg
     1621 aggagcccga ccggcggacg gtgcagagcg ccatcgacgc ggtgatcaag ccgttcgtgc
     1681 gcaagcgcat cctggagcag gaccggcgca ccgacgaccg ctcctcgcag gagatccgcg
     1741 agttgacggc gatggtcggc ctgctgcctc gagcgcatgg ctcggggctc ttccagcgcg
     1801 ggcagacgca ggtgctgacc gcggcgacgc tcgggccgct ggctgaccgc cagcgcctcg
     1861 acaacatctc gccggagcag ttcaagcggt acatgcacca ctacaacttc ccgccgttct
     1921 cggtgggtga ggcgcgtccg ttgcgcggcg ccggccgccg cgagatcgga cacggcctgc
     1981 tcgccgagcg ggcgctcgag ccggggatcc cgtcggccga ggacttcccc tacacgctgc
     2041 ggtgcgtctc ggacgtgctg atgtcgaatg gctcgtcttc gatggcgtcc gtgtgcggct
     2101 cgacgctcgc gctgatggac gcgggcgtgc cgatctcggc gccggtcgcc ggcgtcgcga
     2161 tgggcctggt gacggacgag gcgggcgaga acttccgcgt gctgaccgac attgccggca
     2221 tcgaggacgc gtacggcgac atggacttca aggtcgccgg gacggccgcc ggggtgaccg
     2281 cgatccagct ggacatcaag ctccagcggc tgccggcgga cctccttgag accgtgttcc
     2341 gcgacgcgaa ggcggcgcgt gagcgcatcc tggccgcgat ggctcaggcg atcgaggagc
     2401 ctcgcgagga gctgtccccg tacgctccgc gcacggagac gatcaagatc gaccgcgaga
     2461 agatcggcac ggtcatcggc ccaggcggtc gcgtcatccg cggcatggtc gaggagacgg
     2521 gcgcgacgat cgacgtcgag gacgacggca cgattcacgt cggcggcagc gaccccgagg
     2581 gggtgcgccg cgccatcgag atgatccgcg gtctgaccaa ggaggtcatg gtcggggagc
     2641 agtacgaggg cccggtcacc cgcatcatga ccttcggcgc gttcgtcgag atccttccgg
     2701 gcaaggaggg gctcgtccac atctcggagc tcgcggaaca ccgcgtgaac cgtgtcgagg
     2761 acgaagtgca gatcggcgac cgcgtaaagg tgctggtcgt tgagatcgac aaccttggcc
     2821 gcatcaacct gtcgcggcgc gcgctcctcg agggcggcga cggccgggtc ggcacgcagg
     2881 aaggcgaaga ggaagacttc ttcgacgagg atgagatccc gcggcgcccg gcgcccgggg
     2941 aggcgcctcg atccgtcatg agcggcaccg gcgcctatgg tggcg
//
LOCUS       DAORPG010000189         2967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3359_length_2967_cov_3.417582, whole genome shotgun sequence.
ACCESSION   DAORPG010000189 DAORPG010000000
VERSION     DAORPG010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3359_length_2967_cov_3.417582"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PLX85_05185"
     CDS             <1..507
                     /locus_tag="PLX85_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRC62599.1"
                     /translation="QLHDISTQAAWDEARDEDSGALAGLQIAKYRGLIDHIGVSSHNL
                     DLLPVLITSGAFDAVMLEYSAFFPQTAELIDLAARHDVGVIVMRPLGGSGRTSVMRGR
                     IAEGYDGLLTPANLLRYVLSHPGVSTAIPGARYPSRIHDNVRVASDYTPMTDGERGAI
                     EAEARELY"
     gene            597..2879
                     /locus_tag="PLX85_05190"
     CDS             597..2879
                     /locus_tag="PLX85_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRC62600.1"
                     /translation="MVATEPQGQPLVGTRPIRPDGVDKVTGRARYGSDINLPGMLYGR
                     VKRSPHAHAVIKRIDTSRALALKGVQAVITAHDFQAAEDEVAELGETMSTARWLQDNV
                     LASDKALYVGHAVAAVAATDPHIAEDALDLIDVEYEVLSPVLDVHEAMAEGAPLLHDG
                     LRTVEMAGLFMPGPNQGDQPTNVASHIRFDRGDVEAGFADADVVIEREFRTGVAHQGY
                     IEPHNGTAMWNADNTLTVWLSTQGHFGVRDQLAQVLHLPIGRIRVVPMEIGGGFGGKI
                     PVYMEPMAAIMSRMTGKPVKMLMSRQEVFEGTGPTSATYIRCKVGAKRDGTITAAQAY
                     LAFEAGAYPGSPVGAAAACMFSTYDIPNQLIDGYDVVVNKPKVQAYRAPGSPQGAFAM
                     ESILDEIAERLELDPLEFRLKNASSEGSRRTDGAQFPRIGAAEVMQAALDSPHYRSEL
                     SGEHTGRGVAVGFWFNGGGESSAYAAVNADGTVSLTTGSVDIGGQRAGLAMQFAQNMG
                     IPYEDVRSYVADTDSIGYTRVTGGSRTTFASGWAVHEAAADLRQRLEERAASIWEVDR
                     ELVSYAGGVMSGPDGRQMTFKELAAQLPHTGGMLQGHADASPSTIGPAFACHIVDLKV
                     DPDTYKVEVMRYTAIQDVGTAIHPSYVEGQIQGGAAQGIGMALNEEYRFDASGRMLNS
                     SLLDYRMPTALDVPMLETILVEVPNPGHPYGVRGVGEVPIVPPLAAVANALTHATGTR
                     ITTLPASPERLLEWVADESV"
BASE COUNT          501 a         1047 c          980 g          439 t
ORIGIN      
        1 cagctgcacg acatctccac gcaggccgcc tgggacgagg caagggatga ggacagcggc
       61 gcgctcgccg gcctccagat cgcaaagtac cgagggctca tcgaccacat cggggtgtct
      121 tcgcacaacc ttgacctgct gcccgtgctc atcacgtcgg gcgccttcga cgcggtcatg
      181 ctcgagtact cggcgttctt cccgcagacg gcggagctaa tcgacctcgc ggcccggcac
      241 gacgtcggcg tcatcgtcat gcggccgctc ggcgggtcgg gccgcaccag cgtcatgcgc
      301 ggccgcatcg cggagggcta cgacggcctg ctgacacccg cgaacctgct gcgctacgtc
      361 ctttcgcacc ccggcgtgtc gaccgcgatc cccggcgccc gctacccctc gcgcatccac
      421 gacaacgtgc gcgtcgcctc ggactacacg ccgatgaccg acggcgaacg cggtgccatc
      481 gaggccgaag cgcgcgagct gtactagctt cggggtcatc acggtcgcga ttccggtatc
      541 atcgtcgagc cgttctcgac ggcgtcgtta gccaaactca gcgcaggagg aatgcgatgg
      601 ttgccaccga accacaggga cagccgctgg tcggcacgcg gcccatccgg ccggatggcg
      661 tcgacaaggt gaccgggcgc gcgcgctacg gcagcgacat caacctcccg ggcatgctgt
      721 acgggcgggt gaagcgcagc ccgcacgcgc atgccgtgat caagcgcatc gacacctcgc
      781 gcgcgctcgc gctcaaggga gtccaggcgg tcatcaccgc gcatgacttc caggctgccg
      841 aggacgaggt cgccgagctg ggcgagacga tgtccacggc gaggtggctg caggacaacg
      901 tgctcgcctc ggacaaggcc ctgtacgtag ggcacgccgt tgcggcggtc gccgccaccg
      961 acccgcacat cgccgaggac gcgctcgacc tcatcgacgt ggagtacgag gtgctgtcgc
     1021 ccgtcctcga tgtgcacgag gcgatggcag aaggcgctcc gctgctccac gacggcctgc
     1081 gcaccgttga gatggccggg ctcttcatgc cgggtccgaa ccagggcgat cagcccacga
     1141 acgtcgcgag tcacatccga ttcgaccgcg gcgacgtcga agccggcttc gccgacgccg
     1201 acgtcgtgat cgagcgcgag ttccgcaccg gcgtcgcgca ccagggctac atcgagcccc
     1261 acaacggcac ggcgatgtgg aacgccgaca acacactcac cgtctggctg agcacgcagg
     1321 gccacttcgg cgtccgcgac caactcgcgc aggtgctgca cctgccgatc ggccgcattc
     1381 gtgtcgtgcc gatggagatc ggtggcggct tcggcggcaa gatccctgtc tacatggagc
     1441 cgatggctgc gattatgtcg cggatgaccg gcaagccggt caagatgctg atgagccgtc
     1501 aggaggtttt cgagggcacc ggtccgacct cggcgaccta catccgctgc aaggtcggcg
     1561 cgaagcggga cggcaccatc accgctgcac aggcctacct cgccttcgag gcgggcgcct
     1621 accctggctc gccggtcggc gcggcggcgg cctgcatgtt cagcacctac gacatcccga
     1681 accagctcat cgacggctac gacgtcgtcg tgaacaagcc caaggtacag gcgtaccgcg
     1741 cccccggttc gccgcagggc gcattcgcca tggaatcgat cctcgacgag atcgccgaac
     1801 gcctcgagct cgacccgctg gagtttcgcc tcaagaacgc atcctccgag ggcagccgcc
     1861 gcaccgatgg cgctcagttc ccgcgcatcg gggccgccga ggtgatgcag gccgccctcg
     1921 acagtccgca ctatcgatca gagctctccg gcgagcacac gggccgcgga gtcgcggtcg
     1981 ggttctggtt caacggaggc ggtgagtcct cggcgtacgc cgcggtcaac gccgacggca
     2041 ccgtctccct caccacgggc tcggtcgaca tcggtggcca gcgggccggg ctcgcgatgc
     2101 agttcgccca gaacatgggc atcccctacg aggacgtccg gtcctacgtc gcggataccg
     2161 actcgatcgg ttacacccgc gtcacgggcg ggagccgcac gaccttcgct tcaggctggg
     2221 cggtgcatga ggcggcggcg gacctgcgcc agcgtctcga agagcgcgcc gcctcgatct
     2281 gggaagtcga ccgcgagctc gtgtcgtacg ccggcggcgt catgagcggg cccgacggca
     2341 ggcaaatgac gttcaaggaa ctcgcggcgc agctgccgca caccggcggc atgctgcagg
     2401 gccacgccga cgcctcgccc tcgacgatcg ggccagcctt tgcctgccac atcgtcgacc
     2461 tcaaggtcga cccggacacc tacaaagtcg aggtgatgcg ctacaccgcg atccaggacg
     2521 tgggcaccgc cattcatccc tcgtacgtcg aggggcagat ccagggtggg gcggcgcagg
     2581 gcatcggaat ggcgttgaac gaggagtacc gcttcgacgc cagcgggcga atgctgaact
     2641 cgtcgctgct cgactaccgc atgccgaccg ccctcgacgt gccgatgctc gagacgatcc
     2701 ttgtggaggt gccgaatccc ggacacccgt acggagtgcg cggcgtcggt gaggtcccga
     2761 tcgtgccgcc gctggcggcc gtcgcgaacg cgctgacgca tgccaccggc acgcgcatca
     2821 ccacgctacc ggcctcaccg gagcgactgc tggaatgggt ggcggacgag tccgtttagc
     2881 gccatcctac cgggcgaacc acatccgacc gccctggggc accccccagg gcggcgtctc
     2941 ttttgtcgac tcggaggatt cgatgac
//
LOCUS       DAORPG010000190         2964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3367_length_2964_cov_4.085940, whole genome shotgun sequence.
ACCESSION   DAORPG010000190 DAORPG010000000
VERSION     DAORPG010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3367_length_2964_cov_4.085940"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1610)
                     /locus_tag="PLX85_05195"
     CDS             complement(54..1610)
                     /locus_tag="PLX85_05195"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRC62601.1"
                     /translation="MRTRYVKEWEMMLRRVGIGSVTVALAVAVLLLKPAPGGTADSLP
                     AITNVQVNQTANVTFPHNGQNQPTIAQNPTNALNLIAASNDELAPNGQGTGFYASHDG
                     GHTWPCQGYIDLSPFGKVTFGDAWQTFDGAGNAYLSTIALPLIGPGERLRFDTADIFV
                     AKSTDGGCTYPTVSKVASNSQNISDDKPAIAADTNPGSPHANNLYVAWTQYKDAARGA
                     DEDSKVQVVVARSSDGGATWSKPVGLSAETRAVYPGIPPRQGANVKVGPDGTVYVTWA
                     DTIDGLFVQRIAMSKDGGKKFSDPITVAPIMDEFSFPGGATFRQLGRVLPSFSISPEG
                     SLYLAWAERQLGHTSVVLTKSTDKGASWSHPAVVADVPGRSAFFVAVAAAPSNAVDVA
                     FLAMDDRSDETAPGAGVVSYDAYVTQSLNGGSSFGAPFKISTASSDPDVSSSHSLAVQ
                     FLGDYIAAVADASHLYVVWTDTRNGATCAAVDAFRLQVLTTGTGTPPSLADCPSGFGN
                     ADIYLGTVTP"
     gene            1717..2520
                     /locus_tag="PLX85_05200"
     CDS             1717..2520
                     /locus_tag="PLX85_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62602.1"
                     /translation="MGVKRRWFARIQAPALRAGVLASMVLLFGGCVRAEIAVHVDDDG
                     SGTASVLFGFKKSLLDLMSSLDESGSGAPFDPADVFSDVDRGSLPEGTKVEPYDADGF
                     VGSRITMPFRDVQELPDLLKRVTSDATVPGETAPAGSDGGFERFAIARTEQGWRLDAV
                     AAPLASEGDVSADDEFTQQFLKDASFTIKVRLPGKVGAHNADEVHGNELTWKLDLQST
                     QPRELRAETSGSGSGSGLPWVLIGGGVVGALALGGIGWDIRRRKERHEG"
     gene            complement(2595..>2964)
                     /locus_tag="PLX85_05205"
     CDS             complement(2595..>2964)
                     /locus_tag="PLX85_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62603.1"
                     /translation="GPSGATGPSGPAGPTGPTGANGPTGPSGPAGPTGPTGPVGMMGT
                     PYIVTNDSTAPDGTTIAWCEPGDYVLGGGFERFAFGPGGAGGFIVNKPIEGEGRQGWL
                     IGYGWPATAYAICASIQVPN"
BASE COUNT          459 a          911 c         1079 g          515 t
ORIGIN      
        1 atgggtggcg gttcgcgagg aggcgggagg aactcccgcc tcctcgatga ccgttacggc
       61 gtgacggtgc cgaggtagat gtcggcgttc ccgaagccgg acgggcagtc tgccaggctc
      121 ggcggcgtgc cggtgccggt cgtgaggacc tgcaaccgga aggcgtcgac ggccgcgcac
      181 gtggcgccgt tccgcgtgtc ggtccacacg acgtacagat gcgaggcatc ggcgacggca
      241 gcgatgtagt cgcccaggaa ctgcacggcg aggctgtgcg agctggacac atcagggtcg
      301 gagctggccg tggagatctt gaacggggcg ccgaaggagg agccaccgtt caaggactga
      361 gtgacataag cgtcatacga aacgacgccc gcaccgggtg cggtctcgtc cgagcggtcg
      421 tccatcgcga ggaacgcgac atcgacggcg ttcgacggtg cggccgcgac ggcaacgaag
      481 aacgcgctgc gtccgggcac atcagcgacc acggccgggt gggaccagct cgcgcccttg
      541 tcggtcgact ttgtcagcac cacggacgtg tggccgagct ggcgctcggc ccaggcgagg
      601 tagagcgagc cctcgggcga gatgctgaag gacggcaaca cgcgccccaa ctggcggaag
      661 gtagcgccgc cggggaacga gaactcatcc atgatgggcg cgacggtgat cgggtcgctg
      721 aacttcttgc caccgtcctt cgacatcgcg atgcgctgca cgaagaggcc gtcgatggtg
      781 tcggcccagg tgacgtagac cgtgccgtcc gggccgacct tcacgttcgc gccctggcga
      841 ggcggaattc cggggtagac cgcccgcgtc tcggcgctca gcccgaccgg cttgctccac
      901 gtggcgcctc catccgagga gcgagccacg accacctgta ccttgctgtc ctcgtctgcg
      961 cctcgagcgg catccttgta ctgagtccag gcgacgtaaa ggttgttggc atgcgggctg
     1021 cccgggttcg tgtcggcggc gatggccggc ttgtcgtccg agatgttctg cgagttactg
     1081 gcgaccttgc ttactgtcgg gtaggtgcag ccgccgtccg tcgacttcgc cacgaagatg
     1141 tccgcggtgt cgaagcgcag ccgctccccc gggccgatga gcggcagggc gatggtgctc
     1201 aggtacgcgt tgccggcgcc gtcgaacgtc tgccatgcat cgccgaaggt gaccttgccg
     1261 aaggggctga ggtcgatgta gccctggcac ggccaggtgt ggcccccgtc atgcgacgca
     1321 tagaagcccg tgccctgacc gttgggcgcc agctcgtcgt tcgaggctgc gatcaggttc
     1381 aatgcgttcg tcgggttctg cgcgatagtg ggctggttct ggccgttgtg cgggaacgta
     1441 acgttcgcgg tctggttgac ctggacgttc gtgattgccg gcaggctgtc cgccgtccct
     1501 ccgggcgccg gcttcaagag cagcaccgca acggccaggg cgaccgtcac gctgccgatt
     1561 ccgacccgtc gcagcatcat ctcccactcc tttacgtagc gagtgcgcac gcctccgccg
     1621 cagtatgccg cgaatagtgg cgtgagagtg cgctacacgc gatatttcct cgattgttct
     1681 cggcgtaaca cgtcatgccg gggcgggagt acaggggtgg gtgtgaagag acgttggttc
     1741 gctcggatcc aggcgccggc gctgcgtgcg ggtgtgctcg catcgatggt gttgctgttc
     1801 gggggttgtg tccgcgccga gatcgccgtg cacgtcgatg acgacgggtc ggggacggcc
     1861 tcggtgttgt tcggcttcaa gaagtcgctg ctcgatttga tgagctcgtt ggacgagagc
     1921 gggtcgggtg cacctttcga cccggccgac gtgttcagtg atgttgaccg gggttctctg
     1981 ccggagggca ccaaggtcga gccctacgat gcggatggct tcgtcggctc tcgcattacg
     2041 atgccgttcc gcgacgtgca ggaactgccg gacctgctga agcgcgtcac aagcgatgcc
     2101 accgtgcccg gcgagacggc gcctgccggc tcggatgggg gcttcgagcg gttcgcgata
     2161 gcacgcacgg agcaggggtg gcgcctcgat gccgtggcag cgcctctggc gtccgagggg
     2221 gacgtgagcg cggacgacga gttcacgcag cagttcctga aggatgcttc gttcacgatc
     2281 aaggtccggc tgccgggcaa ggtgggtgcg cacaacgcgg acgaagtcca cggcaatgaa
     2341 ctgacctgga agctggacct gcagtcgacg cagccccgag agctgcgggc ggaaacgagt
     2401 gggtcgggat caggatccgg gctgccgtgg gtgcttatcg gcggcggcgt cgttggcgcc
     2461 ctggcgctcg gcggcatcgg ctgggacatc cggcggcgga aagagcggca cgagggttaa
     2521 cagtcgtccg agggtcggat acgagaaagg gagcggcgaa ccgctcccct tcttgtatcc
     2581 accagcatgg ggccttagtt gggcacctgg atgctggcgc agatggcgta cgcggtggct
     2641 ggccagccat agccgatcag ccatccctga cgcccctcgc cctcaattgg cttgttgacg
     2701 atgaagccac cggctccacc cggcccgaac gcgaagcgct cgaagccgcc tccgaggacg
     2761 tagtcacccg gctcgcacca ggcgatcgtc gtgccgtcgg gcgctgtgct gtcgttcgtg
     2821 acgatgtacg gcgtccccat catgccgacg gggccggtcg ggccggtcgg gcctgccggt
     2881 cctgagggac cagtggggcc attcgcgcca gtgggtccag tcggaccggc ggggccggaa
     2941 ggtccggtcg ctccgctggg gccg
//
LOCUS       DAORPG010000191         2964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3368_length_2964_cov_3.582331, whole genome shotgun sequence.
ACCESSION   DAORPG010000191 DAORPG010000000
VERSION     DAORPG010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3368_length_2964_cov_3.582331"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..895
                     /gene="uppS"
                     /locus_tag="PLX85_05210"
     CDS             119..895
                     /gene="uppS"
                     /locus_tag="PLX85_05210"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017832411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HRC62604.1"
                     /translation="MLKRLIYRYYERGLATEVARGRLPHHVGVVLDGNRRFALKRGLA
                     RAADGHRFGADNADHLVTWCDELRIPVVTLWALSTDNLRRPPEELEAIYAIVEDKVEQ
                     LAEMQARSSFPRRVRAVGRRELLPSSTQAAIQRVEDATAGNAPNELIVAVGYGGREEI
                     TDAVRRMIADRLVRGDSLEGIASGLHPDEISRYLYAPDVPDPDLIIRTSGELRLSGFL
                     LWQSAYSEYYFCDAFWPEFRRIDFLRALRSYAQRERRRGG"
     gene            complement(915..2558)
                     /locus_tag="PLX85_05215"
     CDS             complement(915..2558)
                     /locus_tag="PLX85_05215"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRC62605.1"
                     /translation="MPDVRALARERIAHERGATVKDWGGRIAVAIVYPNSYYIGMSNL
                     ALQTVYSLFNAYPDFVCERAFYDPPSRDARHPYPVVSMESQRSLADFAVLAFTLSYEF
                     DYFNMVQTLRSAGIPLYSEQRDERHPLVIAGGACMLTNPAGVAPFLDVAVVGEGEEVI
                     PALVAAWRDGLYGSRDELLDELAEIPGLYVPSRPPIAGAVERQWVHDISSTEAYSTIL
                     TADTELSDMTLIEVARGCGRGCRFCIAGYVFRPPRYRSKEQLLSIVEHSLAHTPRVGL
                     LGAAVADHPELSELVVGAHELGARVSVSSMRVDNLDPRVLRALAAGGTKTVTVAPEAG
                     SERMRLAINKGVTEEQILEGAARVGEAGAARFKLYFMIGLPGEEDDDVYAMAALGNKI
                     KDRLDRTGSGTRLVLSVSPTIPKPWTAYQYEVQAAPDVIKRRQGILKQHLAPGIDLRS
                     DSPMTARVDDVLSRADARLAPMLESMTDNSLGAYRRAIAEHGLDGPLPWEGERPPWFI
                     VDVGMTERFLLREMDKHERVRRTIPCPPPEVGCKLCGVC"
     gene            complement(2597..>2964)
                     /locus_tag="PLX85_05220"
     CDS             complement(2597..>2964)
                     /locus_tag="PLX85_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62606.1"
                     /translation="AGSTSRRAADADGRAHRLAAAGRYTGNMYRSEPPRPEPKGSWRE
                     IAQFSWIALSIVLPIVGLVVLVMLVAASFFVLLSIQPLLALIPVAVVAAGFVGFILID
                     RRNQRRLEDEIARGGTSRR"
BASE COUNT          481 a         1027 c          982 g          474 t
ORIGIN      
        1 cacatgcgcg gacgccgcgt cctcaccggc gccgcgtcct gatgcaaagg gggaggggcc
       61 attcgggatt ccgattggcc ctccgcctac gatgtgacgg agagcgagga ggttcggctt
      121 gctgaaacgg ctcatctacc gctactacga gcgtgggctg gctacggaag tcgcgcgcgg
      181 tcgactgccc caccacgtcg gcgtggtgct ggatggcaac cgccgtttcg cgctcaagcg
      241 cggactggcc cgcgccgccg acggccaccg ctttggggcg gataacgctg accacctcgt
      301 cacctggtgc gatgagctgc gcatcccggt cgtcaccctg tgggcgctct cgaccgataa
      361 cctccgccgc ccgccggagg aactcgaggc gatctacgcg atcgtcgagg acaaggtcga
      421 gcagctggcc gagatgcagg ccaggtcctc gttcccccgc cgcgtgcgag ccgtcggccg
      481 gcgcgagctc ctaccgtcgt cgacccaggc agcgatccag cgcgtggaag acgccaccgc
      541 tggcaacgcg ccaaacgaac tcatcgtcgc agtcggctac ggtggccgcg aggagatcac
      601 cgacgcggtg cgccggatga tcgccgaccg cctggtccgc ggggactcgc tcgagggcat
      661 cgcgagcgga ctccaccccg acgagatcag tcgctacctc tatgccccgg acgtaccgga
      721 tccggacctg atcattcgca catcgggcga gttgcggttg tccggattcc tgctctggca
      781 gagcgcctac agcgagtact acttctgcga cgcgttctgg ccggagtttc ggcgcatcga
      841 tttcctgcgt gcgttgcgct cgtacgcaca gcgtgagcgc cgccggggcg gatgacgccg
      901 tccctcgttg gcgctcagca caccccgcag agcttgcacc cgacctcggg cggcgggcac
      961 gggatcgtgc gccgaacgcg ctcgtgcttg tccatctcgc ggagcaggaa ccgctccgtc
     1021 atgccgacat cgacgatgaa ccagggcggc cgttcgccct cccacggcag cgggccgtcg
     1081 aggccgtgct cggcgatcgc ccgccggtac gcgccgagtg agttgtcggt catcgattcg
     1141 agcatgggcg cgagccgcgc atccgcccgc gagaggacgt cgtccacgcg tgcggtcatc
     1201 ggcgagtcgc tgcgcaggtc gatcccgggc gccaggtgct gcttgaggat gccctgtcgc
     1261 cgcttgatca cgtccggcgc cgcctgcacc tcgtattgat acgccgtcca gggcttcggg
     1321 atggtggggc tcaccgagag gacgaggcga gtaccgcttc cggtccggtc gaggcgatcc
     1381 ttgatcttgt tgcccagggc cgccatcgca tacacgtcgt catcttcttc gccgggcagc
     1441 ccgatcatga agtacagctt gaaccgcgcc gcgccggcct cgccgacacg cgcggcccct
     1501 tcgaggattt gctcctcggt cacgcccttg ttgatcgcta gccgcatccg ctccgacccg
     1561 gcctcggggg cgacggtgac cgtcttcgta ccgccagccg cgagggcacg cagcaccctt
     1621 gggtcgaggt tgtcgacgcg catcgaggac accgagaccc gcgccccgag ctcgtgggcc
     1681 ccgacgacca gttccgacag ctcggggtgg tcggccactg cggcgccgag caggccgacc
     1741 cgaggcgtgt gtgcgagtga atgctcgacg atcgagagca actgttcctt gctgcgatat
     1801 cgcggcgggc ggaagacgta ccccgcgatg cagaaccggc accctcgccc gcacccccga
     1861 gccacctcga tcagcgtcat gtcggacagc tcggtgtccg ccgtgaggat cgtcgagtag
     1921 gcctcggtgc tgctgatgtc gtgcacccac tgccgctcga ccgcacccgc gatcggtggc
     1981 cggctcggta cgtagagccc cggaatctct gccagttcgt cgagcagctc gtcgcgcgag
     2041 ccgtacagtc cgtcacgcca ggccgccacc agcgcgggga tgacctcctc gccctccccc
     2101 acaaccgcga cgtcgaggaa gggcgcgacc ccggccgggt tcgtgagcat gcacgccccg
     2161 ccggcgatca ccagcggatg acgttcatcc cgctgctccg agtacagcgg gatcccggcg
     2221 ctccgcagcg tctgcaccat gttgaagtag tcgaattcgt acgacagggt gaaagcgagg
     2281 accgcaaagt cagccagcga acgctgcgac tccatcgaga ccacggggta ggggtgtcgc
     2341 gcgtcgcgcg agggtggatc gtagaaggcg cgctcgcaga cgaagtccgg gtacgcattg
     2401 aacaggctgt acaccgtctg cagcgccagg ttcgacatgc cgatgtagta ggagttggga
     2461 tagacgatcg ccacggcgat gcggccgccc cagtccttga cggtggcgcc gcgctcatgc
     2521 gcgatccgtt cgcgtgccag tgctcgtacg tcgggcattt agatagtcta ccgcagctaa
     2581 acgatcgagg cccgggtcag cggcgcgagg tgccgcctcg ggcgatctca tcctccagcc
     2641 gccgctggtt gcgccggtca atcaggatga acccgacgaa tccggccgcc acgaccgcga
     2701 ccggaatcag tgcgagcaga ggctggatcg acagcaacac gaagaacgag gccgcgacca
     2761 gcatcacgag gaccaccagt ccgacgatag gcagcacgat gctgagcgcg atccagctga
     2821 actgcgcgat ctcgcgccag ctacccttcg gttcaggacg cggtggttct gaccggtaca
     2881 tgtttccagt gtagcgaccc gcggcggcga gccggtgggc gcgcccatcg gcatcagccg
     2941 ctcgtctgct cgtcgaacca gctc
//
LOCUS       DAORPG010000192         2964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3369_length_2964_cov_3.561705, whole genome shotgun sequence.
ACCESSION   DAORPG010000192 DAORPG010000000
VERSION     DAORPG010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3369_length_2964_cov_3.561705"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLX85_05225"
     CDS             complement(<1..488)
                     /locus_tag="PLX85_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62607.1"
                     /translation="MVTVPALPEIAAQVAGIRARAGSGCFLGIRLPSSFNPVDLLHDV
                     VANQVVASYERPDGGLALVAVGEAGRADAQPGEVPQAVREQVRALLRAEVVSEAPELR
                     PRLLGGFAFDVAHAPTGPWAGFAAGSLVLPRLLFVRDGGVTGVVVAPGVEPAEIEALG
                     LKG"
     gene            complement(624..1130)
                     /locus_tag="PLX85_05230"
     CDS             complement(624..1130)
                     /locus_tag="PLX85_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62608.1"
                     /translation="MPYLTRQYGSNARWLTKALREVPHEIHELLTSMDERELRWRPED
                     GDWCAKEVAAFLLESEREDLRAVEAILRRDGAPIEERRAYLAPGEHDYLDESIDELVW
                     DFMTERESLLWSLEWSDAWEHAGEHPYRGRVPLLQYLHEINERDLEAAWRLRKLQEAL
                     GAGAVRRR"
     gene            1244..2077
                     /locus_tag="PLX85_05235"
     CDS             1244..2077
                     /locus_tag="PLX85_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62609.1"
                     /translation="MSADSLPYTLLILLVELAVGSLAVTVFFDQRRMVTRGYVQMGAM
                     VVVPTALLALLVAFGVRVEAEIDGYFFSTGWFDPFRWSLVMFLVTSAAFMVAAFAGRR
                     DVGLMLGAAGIAVGVVVLVLLAGLVAPPAWSFPGVIASMLAAALALGGSLMAMSWGHW
                     YLTNSGLPKEPLEQMSLLVVGALGLQLVLLVVGVIAPVREVPLTESAFGVSLGANPAF
                     WLRVAVGLLFPLFLSVLAWKAASIRGMMSATGLLYIATGAVLAGEVLARGLLFATGIT
                     L"
     gene            2158..2796
                     /locus_tag="PLX85_05240"
     CDS             2158..2796
                     /locus_tag="PLX85_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRC62610.1"
                     /translation="MADPEPLPIRARFPNVSRVERLDATDDELIASLTAGDISALEVL
                     YDRYSTLVYSVSLRVLYDRQLAEDVTQEVFLRLWRRPQSFDPSRGRLLSWLMSVTRNR
                     AIDERRKLARRARTEDTADDPLPDRSDTDGGDDPQLALVLTELRWKVRAAMQSLPPAQ
                     RQVIELGYFSGLTQVEIAERTGLPLGTVKTRVRLAMNKLRAELNDFQQESSR"
BASE COUNT          425 a         1114 c          926 g          499 t
ORIGIN      
        1 cccttcaggc cgagtgcctc gatctcggcc ggttcgacgc ccggggcgac gaccacgccc
       61 gtgacgccgc cgtcacgtac gaacagcagc ctcggcagga ccagcgagcc ggccgcgaat
      121 ccagcccacg gcccggttgg cgcatgcgcg acgtcgaagg cgaacccgcc gagcagacgc
      181 ggacgcagtt ccggcgcctc gctcacgacc tctgcgcgaa gcagagcgcg cacctgctcg
      241 cgcactgcct ggggcacctc gcctggctgt gcgtcggccc gccccgcctc acccaccgcg
      301 acgagcgcga gcccgccgtc gggtcgctcg tacgacgcca ccacctggtt cgcgaccacg
      361 tcgtgcagca ggtcgaccgg gttgaacgag gaaggcagac ggatgccgag gaaacaaccc
      421 gaccctgcgc gcgcacggat cccggcgacc tgcgccgcga tttccgggag tgccgggacg
      481 gttaccacgg agtcactcgg acctctggct ggctgcctgg acacgcgata acggcgcgga
      541 ccatgcgttc ctctgcgggt gcggcagccg gttggacagt acctgtaagt ataaagccag
      601 cggacgtacg cgctgtagtc gcgctaccgg cgtcggacgg cgcccgcgcc gagcgcctcc
      661 tgcagcttcc gcaggcgcca ggccgcctcg aggtcgcgtt cgttgatctc gtgcaggtac
      721 tgcagcagcg gcacccgacc gcgatacggg tgctcgcccg catgctccca ggcatcgctc
      781 cactcgaggg accagagcag cgactcccgt tccgtcatga agtcccatac cagctcgtcg
      841 atcgactcat cgaggtagtc gtgctcaccc ggcgcgaggt acgcccgccg ctcctcgatt
      901 ggggcgccgt cccgccgcaa aatcgcctcg accgcccgca ggtcctcccg ctcgctttcg
      961 agcaggaagg ccgccacctc cttggcgcac cagtcgccat cttccggccg ccagcgcagc
     1021 tcgcgctcat ccatgctcgt gagcagctcg tgaatctcat gcgggacctc gcgcagggcc
     1081 ttggtcagcc agcgggcatt cgagccgtac tgccgcgtca gataaggcat acctgcatcg
     1141 tgatcgccgt tcgcccggcc tgtcaacgcc gaactgtcca cccgcccagc acgcctcgcg
     1201 tgcgttgacc ccccgcgaca ggctctctac gatccgggct cccgtgagcg ctgattccct
     1261 cccctacacc ctgttgatcc tgctggtcga acttgcagtc ggctcactcg ccgtgaccgt
     1321 cttcttcgac cagcgccgca tggtcacgcg aggttatgtc cagatgggtg cgatggtcgt
     1381 cgttccgacg gcgttgctcg cgctcttggt cgccttcggc gtacgcgtcg aggcggagat
     1441 cgatggctac ttcttcagca ctggctggtt cgatccgttc cgctggagcc tcgtcatgtt
     1501 cctcgtgacg agcgcggcgt tcatggtcgc ggccttcgcc gggcgccgcg acgttggact
     1561 gatgctcggc gctgccggca tcgcggtcgg ggtcgtcgtc ctggtactgc tcgccggcct
     1621 cgtcgcccct cccgcgtggt cgttcccggg cgtcattgcg agcatgctcg cggcggcgct
     1681 cgccctcggc ggctcgctca tggcgatgtc ctggggccac tggtatctca cgaactccgg
     1741 cctcccgaag gagccgctcg agcagatgtc actgctcgtc gtcggcgcgt tggggctcca
     1801 actcgtgttg ctcgtcgtgg gtgtgatcgc accggtccgc gaggtgccgc tcaccgagtc
     1861 cgccttcggc gtctcgctcg gcgcgaaccc ggccttctgg ctgcgcgtcg cggtcgggct
     1921 cctgttcccc ctcttcctct ccgtcctcgc ctggaaggcc gcgagcatcc gaggcatgat
     1981 gtccgccacc ggcctgctgt acatcgccac cggcgcggtc ctggctggcg aggtgctcgc
     2041 ccgcgggctc ctgttcgcga cggggatcac cctctgatcc cggaccctag accatcacgt
     2101 cagtcgtaga ctagaatcac cgcttccact cggcgcctgg gggatggggg gcatcccttg
     2161 gctgaccctg aaccactgcc aatccgtgct cgcttcccga acgtatcacg agtagagcgg
     2221 ctcgacgcga ccgatgacga gctgatcgcg agcctcaccg ctggcgacat ctccgccctc
     2281 gaggtcctgt acgaccgcta ctcgaccctc gtgtactcgg tgagcctgcg tgtcctctac
     2341 gaccgccagc tcgccgagga cgtcacgcag gaggtgttcc ttcgtctctg gcggcggccg
     2401 cagtcgttcg accctagccg cggccggctc ctcagctggc tcatgtcggt gacccgcaac
     2461 cgcgccatcg atgagcgtcg caagctcgct cgacgcgccc gcaccgagga caccgcggac
     2521 gatccgctgc cagaccgaag cgacaccgac ggcggggacg acccgcaact cgcgctcgtc
     2581 ctgaccgaac tgcgttggaa ggtgcgcgcc gccatgcaga gcctcccacc ggctcagcgc
     2641 caggtcatcg agctcggcta cttcagcggc ctcacacagg tcgagatcgc cgagcgcacc
     2701 ggactccccc tgggcaccgt gaagacgcgc gtccggctcg cgatgaacaa gctgcgcgcc
     2761 gagctcaacg acttccagca ggagtcatcc cgatgactcc cgaggaacgc gaggagctgc
     2821 tcgccgccta cgcgctcggc accctcagcg ccccggacgc cgcggccgta gaacagctcg
     2881 tgcgcagcga cagcgcggcc gcggccgacc tcgccgccta ccacgagatg gtcgacctga
     2941 tcgctgtcag cgctcccctt cgcc
//
LOCUS       DAORPG010000193         2962 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3376_length_2962_cov_3.456140, whole genome shotgun sequence.
ACCESSION   DAORPG010000193 DAORPG010000000
VERSION     DAORPG010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3376_length_2962_cov_3.456140"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PLX85_05245"
     CDS             <1..834
                     /locus_tag="PLX85_05245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRC62611.1"
                     /translation="PSIVPLPSAPAPRSRGITPPAPTSASVLLLDDASGGVLFERNGH
                     QPLAPASLTKIATAIVAIEQSDLEAVADIDVDSRTMRGSTVMGLEPGDRFSLRDLLYG
                     LMLPSGNDAALAIGRQVAGSDAAFVARMNDLVARLGLRDSHFVNPHGLGGAGHVTSAY
                     DLAMLSRYAMRLPAYREIASADMWVARGSRELTLFSLVSTDRWTIPGVDAGKSGYTRA
                     AGRTLVLTAERDGHRLHVVVMNDPQTEQVAAALLEWGYANFEWPTPAAVAAAIASEPA
                     P"
     gene            complement(860..1444)
                     /locus_tag="PLX85_05250"
     CDS             complement(860..1444)
                     /locus_tag="PLX85_05250"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRC62612.1"
                     /translation="MPDSAVAALDAFIEEQSVDKSKANWRTRLSPPPMVDLDSGSTYY
                     WDLETNVGSISIRLMPDVAPMHCISTMYLTRLGFYDGLSFHRVITQFMAQGGCPLGTG
                     TGGPGYQYAGEFSNAVRHDRPGLLSMANAGPRTDGSQFFLTFVPTPWLDGNHTIFGEV
                     VDGMDTLRALEARGSQGGSTTEPLAITRATVRVE"
     gene            complement(1509..1796)
                     /locus_tag="PLX85_05255"
     CDS             complement(1509..1796)
                     /locus_tag="PLX85_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62613.1"
                     /translation="MHATVRQYEGVENPAEVIRQVGETFLPLQHDIPGFVSYYFVDVG
                     EAGGRMVSVSVFETEEGTAESNRRAASWVAEHPNLIPPAKSAEAGPVVVGG"
     gene            complement(1886..>2962)
                     /locus_tag="PLX85_05260"
     CDS             complement(1886..>2962)
                     /locus_tag="PLX85_05260"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRC62614.1"
                     /translation="LVLDLQRPEAQALVQRVIPHFDVFIINSRAGVPGRLGVDYETLA
                     KSRPDLVYMENTGYGDEGPSAQRAGSDIVAQAYSGLMAGEGKVNEFGAPRQITSTAIG
                     DFATGLAAAMGICAALYRRALSGRGDHIKTSLLATALSIQSGTVGRLPVSDEIYQRPL
                     IEEVERLRAAGAPWADIVKARDARRRTGGAFANYYNTYRVKDGAITLGALTPRNQQQI
                     RDVLGITEDPMLHPDFNAADPAWAAKVEALATDIRARMLTRTMDEWIEAFDAAGAPAS
                     KVNIPEDLADDPQVRAMGYMIEVDHSITGPERWPGAVTRHANFPTGTSRPAPALGQHT
                     DESLLELGLTAEEVAELRRCGAAE"
BASE COUNT          444 a          964 c         1050 g          504 t
ORIGIN      
        1 ccctcaatcg tcccgctgcc ctcagcgccc gcccctcgca gccgaggcat cacgccgccg
       61 gcgccgacga gcgcgtcggt cctgctgcta gacgatgcct ccggcggggt gctgttcgag
      121 cgtaacggtc atcagccgct ggcccccgca agcctcacca agatcgcgac ggcgatcgtc
      181 gcgatcgagc agagcgacct cgaggcggtg gccgacattg acgtggatag ccggacgatg
      241 cgtgggagca cggtcatggg gctcgagccc ggcgaccggt tttcgttgcg tgacctgctg
      301 tacggactga tgttgccgtc cggcaacgat gcggccctgg ccatcgggcg gcaggtggcg
      361 ggctcggatg ccgcgttcgt ggcgcggatg aacgatctcg tcgcccgcct cggcctgcgc
      421 gacagccact tcgtcaaccc gcacgggttg ggcggcgccg gtcacgtcac ctcggcgtat
      481 gacctcgcga tgctgtcgcg ctacgcgatg cgcttgcccg cgtaccgcga gatcgcgagt
      541 gctgatatgt gggtagcgag ggggtcgcgg gagctgacgc tcttcagcct cgtcagcacc
      601 gatcgctgga ccatccccgg tgtggatgcg ggaaagagcg ggtacacgcg agccgcaggc
      661 cgcacgctcg tgctgaccgc agagcgcgac gggcaccggc tgcacgtcgt cgtgatgaac
      721 gatccgcaga ccgagcaggt cgcggcggcg ctgctcgaat gggggtacgc gaatttcgaa
      781 tggcccacgc cagcggcggt agcggccgcg atcgcctcgg agccggcgcc gtagcgaggg
      841 ctccgaggcg gggtcggtgc tattcgacgc ggacagtcgc gcgcgtaatc gcgagcggct
      901 ctgtcgtgct gcctccctgc gagccgcggg cctcgagcgc gcggagtgtg tccatcccgt
      961 cgaccacttc gccgaagatg gtgtggttgc catcgagcca gggcgtgggc acaaaggtga
     1021 ggaagaactg gctgccgtcc gtgcgcggcc cggcgttggc catgctgagc aagccaggcc
     1081 ggtcatggcg gacggcgttg ctgaactcgc cagcgtactg gtagcccgga ccgccggtcc
     1141 cggtgccgag cgggcagccg ccctgcgcca tgaactgagt gatcacgcgg tggaaactca
     1201 ggccgtcgta gaaaccgagc cgggtgaggt acatcgtgct gatgcagtgc atgggcgcca
     1261 catccggcat caggcggatg ctgatggagc cgacattcgt ttcgaggtcc cagtagtagg
     1321 tggaacccga atcgaggtcg accatcggcg gcggactgag gcgggtgcgc cagttcgcct
     1381 tcgacttgtc gaccgactgc tcctcgatga acgcatcgag cgccgcgacc gcggaatctg
     1441 gcacaacgaa tctcctttcg cccggattcg ggccgggacg tgaatgaacg gagtggccgg
     1501 gtcgggccct atccgccgac cacgactggc cccgcctcgg cggacttcgc cggcgggatc
     1561 aggttcggat gttccgccac ccaggacgcg gcgcgccggt tcgactcggc ggtgccttct
     1621 tcggtctcaa agacgctgac ggagaccata cggccaccgg cttcgccgac gtcgacgaag
     1681 tagtagctga cgaagccagg gatgtcgtgc tgcagcggga ggaacgtctc tccgacctgc
     1741 cggatcacct cggccgggtt ctcgaccccc tcgtattgcc tgaccgtcgc gtgcatccct
     1801 gcctcctcct gtgcgtgcgg tcgtgcgatt ctacggcacg tttcggctgg cctcgatctc
     1861 cctgcaggag cgaccgccgg gccggctact ccgccgcgcc gcagcgccga agctcggcga
     1921 cctcctcggc ggtgagcccg agctcgagca gtgactcgtc ggtgtgctgg ccgagagcgg
     1981 gcgcgggacg cgatgtcccg gtagggaagt tcgcgtgacg ggtgacggcg ccgggccagc
     2041 gttccgggcc ggtgatcgag tggtcgacct cgatcatgta gcccatcgcc cgcacctgcg
     2101 gatcgtcggc gagatcctcc ggaatgttca cctttgaggc gggcgcgccg gcggcgtcga
     2161 aggcctcgat ccactcgtcc atcgtgcgtg tcagcattcg tgctcgaatg tctgttgcga
     2221 gagcttcgac cttcgccgcc catgcggggt cagcggcgtt gaagtccggg tgaagcatcg
     2281 gatcctcggt gatgccgagg acatcgcgga tctgttgctg gttgcggggg gtgagcgcgc
     2341 cgagggtgat cgcgccgtcc ttcactcgat acgtgttgta gtagttggcg aaggcgccgc
     2401 cagtcctccg cctcgcgtcg cgggccttca cgatgtccgc ccacggggcc ccggcggcgc
     2461 gcagacgctc gacctcctcg atcagcgggc gctgatagat ctcgtccgag acgggcaggc
     2521 ggccaacggt cccgctctga atggagagtg cggtggcgag gagagaggtc ttgatatggt
     2581 cgccgcggcc gctgagagcg cggcggtaga gcgcggcaca gatgcccatg gcggccgcga
     2641 gtcccgtggc gaagtcaccg atggccgtgc tggtgatttg cctcggcgcg ccgaactcgt
     2701 tcacctttcc ttcgccggcc atgagccctg agtaggcctg agccacaatg tccgaacccg
     2761 cgcgctgggc gctcggccct tcgtcgccat aaccggtgtt ctccatgtag acgagatccg
     2821 ggcgcgactt cgcgagggtt tcatagtcca cgccaagccg tcccgggacg cccgcacgtg
     2881 agttgatgat gaagacgtca aagtgcggga tgacgcgctg cacgagcgcc tgtgcctccg
     2941 ggcgttgcag gtcgaggacc ag
//
LOCUS       DAORPG010000194         2956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3400_length_2956_cov_3.978973, whole genome shotgun sequence.
ACCESSION   DAORPG010000194 DAORPG010000000
VERSION     DAORPG010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3400_length_2956_cov_3.978973"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="PLX85_05265"
     CDS             <1..141
                     /locus_tag="PLX85_05265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC62615.1"
                     /translation="RRLNPPGSGLGLAIASRIARLHGGNLAIAPRQPRGTAVRIVLPL
                     LG"
     gene            232..1350
                     /locus_tag="PLX85_05270"
     CDS             232..1350
                     /locus_tag="PLX85_05270"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011601760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRC62616.1"
                     /translation="MRVPAASIVFNDDDIAEALSLIESSLRSGQLTLGKHGQAFEEAF
                     AQRCGRAHAVAVSSGTSAIEIVLRACGIESGEVIVPDNTFFATAAAVVHAGARPVFAD
                     TDRDTLAVDVDDVLSRITPQTKAVVVVHIGGIVSPELPRLATECAARGIQLICDAAHA
                     HGATLDGKDASNWGIASTYSFYPTKVMTSGEGGMIVTDDERIADEARVYRDQGKAGFT
                     SNFHTRLGANWRLSEVHAAIGLVQLKRLNEFIERRRQIAAVYDDLLPRETNLTAVPCQ
                     RLDSSNFYKYVAYLPAGVDRAALKATMRERFEVGMSGEVYEFPLHAQPVFEPFVSGSY
                     PVSDDVCARQVCLPVSARMTTDDARYVVESLARTISGG"
     gene            1355..2299
                     /locus_tag="PLX85_05275"
     CDS             1355..2299
                     /locus_tag="PLX85_05275"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRC62617.1"
                     /translation="MNSASTEMRVAVTGGAGFIGSHVVDKLVEAGHPVTVIDAIEPHR
                     PDVDWQPCDVTSFDEVALAVRDASAIYHLAAMANVNDVAAAPLESVQLNVMATANLLE
                     AARREGNKHLIFASTVWVYEAASGTEVDEETPLRPDGTKHLYTAEKAASEMLVHSYSQ
                     LFGVPTTVLRYGIPYGPRMREALVMPIFIKKAMAGEPLTVAGDGLQYRNFVYVEDLAE
                     AHVQVLRHEATGTFNLEGPREVSILEMAETINRLIPTSAGIMHTEARAGDYQGRVVSP
                     ERSARALGWRPTTSFEDGLRRTVDWFVEKWTPVAAATA"
     gene            2349..>2956
                     /locus_tag="PLX85_05280"
     CDS             2349..>2956
                     /locus_tag="PLX85_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC62618.1"
                     /translation="MPAYDEASTIEGVLDDLYPRVDRVIVVDDGSTDGTGPLLDRWAQ
                     DRDRCTVVHLSPNQGLSAALRAGWDRVRDLVAEGVISEDDIAFSIDADGQHEPAALHA
                     LADRLIEGGYALVIGNRDLSYHGSYKRLGNGVMTWIGRLTSGAPLHDIESGYRMFRVG
                     PLLEAQQYYRGYRYSETVEVAVILARLGYRVDNDLRIEVPVAR"
BASE COUNT          478 a         1127 c          897 g          454 t
ORIGIN      
        1 cggcgcctca atccgcctgg ctccggccta ggcctggcga tcgcctcgcg catcgcccgc
       61 ctccacggtg gcaacctcgc catcgccccc cgtcagccgc ggggcaccgc ggtgcgcatc
      121 gtgcttccgc tgctcggcta gccgataacg ccttcaggtt ttcttcaggt ttcgttcagg
      181 ctaccaaccg tcgcaggtga cacgatcccc gccaacaagg ggaggggccg catgcgtgtt
      241 ccagctgctt cgatcgtgtt caacgatgac gacatcgccg aggcgctatc gctgatcgaa
      301 tccagcctcc gcagcggaca gctcacgctc ggcaagcacg gccaggcttt cgaggaggcc
      361 ttcgcccagc gttgcgggcg cgcccacgcc gtcgccgtca gcagcggcac cagcgcaatc
      421 gaaattgtgc tgcgcgcctg cggcatcgaa tctggcgagg tcatcgtccc cgacaacacg
      481 ttctttgcga ccgccgcggc tgtcgtgcat gcgggcgccc ggcccgtctt cgcggacacg
      541 gaccgcgaca ccctcgcggt cgacgtcgac gatgtcctct cccgcatcac cccgcagacg
      601 aaggcagtcg ttgtcgtgca catcggcggc atcgtctcgc cggagctgcc acgcctggcc
      661 accgagtgcg ccgcgcgcgg catccagctc atctgcgacg ccgctcacgc gcacggcgcg
      721 accctcgacg gcaaggacgc ctccaattgg ggcatcgcct ctacgtactc cttctacccc
      781 acgaaggtaa tgacctcggg cgaaggcggc atgatcgtca ccgacgacga gcgcatcgcc
      841 gacgaagctc gggtctatcg cgaccagggc aaggccggat tcaccagcaa cttccacacc
      901 cgcctcggcg ccaactggcg cctgagcgag gtgcacgcgg ccatcgggct cgtgcagctg
      961 aagcggctca acgagttcat cgaacgccgc cgccagatcg ccgccgtgta cgacgacctg
     1021 ctgccccgcg agaccaacct cacggccgtc ccctgccagc gcctcgactc gagcaacttc
     1081 tacaagtacg tcgcctacct gcccgccggc gtcgaccgcg cggctctgaa ggcgacgatg
     1141 cgcgagcgct tcgaggtcgg catgagtggc gaggtctacg agttcccgct ccacgctcag
     1201 cccgtcttcg agccctttgt cagcggctcg taccccgtct ccgacgacgt ctgcgcccgg
     1261 caggtctgcc tgccggtgag cgcccgcatg accaccgacg acgcccgcta cgtcgtcgaa
     1321 agcctcgcac gcaccatctc aggagggtaa cgagatgaat tcagcgtcca cggaaatgcg
     1381 cgtcgccgtc accgggggcg ccggtttcat cggcagccac gtcgtcgaca agctcgtcga
     1441 ggccggccac cccgtcacgg tgatcgacgc aatcgagcca caccgcccgg acgtggactg
     1501 gcagccgtgc gatgtcacca gcttcgacga ggtcgcgctt gccgtgcgcg acgcctccgc
     1561 catctaccac ctcgcggcga tggccaacgt gaacgacgtc gccgcggccc cccttgagtc
     1621 ggtccagctg aacgtcatgg cgacggccaa cctgctggag gcggcgcgcc gcgagggcaa
     1681 caagcacctc atcttcgcct ccacggtatg ggtctacgag gccgcttcgg gcaccgaggt
     1741 ggacgaggaa acgccgctgc ggcccgatgg cacgaagcat ctctacaccg ctgagaaggc
     1801 cgccagcgag atgctcgttc actcctacag ccagctcttc ggcgtgccga ccaccgtcct
     1861 ccgctacggc atcccctacg gcccgcgcat gcgcgaggcg ctcgtgatgc cgattttcat
     1921 caagaaggcg atggccggcg agccgctcac cgtcgccggg gacggcctgc agtaccgcaa
     1981 cttcgtctac gtcgaggacc tcgccgaagc ccacgtccag gtcctgcggc acgaggccac
     2041 cggcaccttc aacctcgagg gccctcgaga ggtctccatc ctcgagatgg ctgagaccat
     2101 caaccgcctg atcccgacca gcgccggcat catgcacacc gaggcgcgcg cgggtgacta
     2161 ccagggccgc gtcgtctccc cggaacgctc ggcacgagcc ctcggctggc gccccaccac
     2221 ctcattcgag gacgggctgc gccgcaccgt cgactggttc gtcgagaagt ggacgccggt
     2281 agccgcggcc accgcctgag cgcgccaccg ccctccgctc gcacgtgagg ccgcgcctca
     2341 tcgcggtcat gcccgcctat gacgaggcga gcacgatcga gggcgtcctc gatgacctgt
     2401 acccccgcgt tgaccgcgtc atcgtcgtcg atgacggctc gaccgacggc accgggcccc
     2461 tgctcgatcg ttgggcacag gaccgcgatc gctgcacggt cgtgcacctt tcccccaacc
     2521 aggggctctc ggcggccctg cgcgccggct gggaccgcgt gcgcgacctc gtcgctgagg
     2581 gcgtcatcag cgaggacgac atcgccttct ccatcgacgc cgacggccag cacgagccgg
     2641 cggcgctcca cgccctcgcc gaccgcctga tcgagggcgg ctacgccctc gtcatcggca
     2701 accgcgacct ctcctaccac ggttcataca agcgcctcgg caacggcgtc atgacctgga
     2761 ttggccggct caccagcggc gccccgctcc atgacatcga gagcgggtac cgcatgttcc
     2821 gcgtcggccc gctcctcgaa gcccagcagt attaccgtgg ctaccgctac agcgagaccg
     2881 tcgaggtggc cgtgatcctg gctcgcctcg gctaccgcgt cgataacgac ctccgcatcg
     2941 aggtccccgt cgctcg
//
LOCUS       DAORPG010000195         2951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3412_length_2951_cov_3.068370, whole genome shotgun sequence.
ACCESSION   DAORPG010000195 DAORPG010000000
VERSION     DAORPG010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3412_length_2951_cov_3.068370"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(304..717)
                     /locus_tag="PLX85_05285"
     CDS             complement(304..717)
                     /locus_tag="PLX85_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62619.1"
                     /translation="MTTATFVCWTTERRDPAALGALLIARELHARIHGHRCRVVDVEP
                     MSDGSVRVTGYEVQEQLWRPSTRRAQTFRADERVTIHRPVYVGDRVAVATNGCRATVE
                     LQSIKSPAAAPTGVLVDYGERRDVVQPDTLSLDDG"
     gene            complement(854..1294)
                     /locus_tag="PLX85_05290"
     CDS             complement(854..1294)
                     /locus_tag="PLX85_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62620.1"
                     /translation="MDTNPRLIASLTKYSRDTLAVARQIAAIVPGGDSASLIGTGGGC
                     EAFEVHVDGYGDSYAWVTVSGDPSVDFDNDSQATVCVYALDEDAYLDGRWLAIDCVPL
                     HRVADTIVEATRRFAEAVKAGKPPVDAYANWPEWADETDALAAS"
     gene            1370..2371
                     /locus_tag="PLX85_05295"
     CDS             1370..2371
                     /locus_tag="PLX85_05295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRC62621.1"
                     /translation="MRPEPWHADNDVTIYHGDALATLRELPDESVQMCATSPPFWALR
                     DYGVDGQLGQEATLKEWAANLVDVFREVRRVLKPDGTLWVEVGDSYNAGTAGRNGGKS
                     GAARDHLTVAYWRKAGQMGDRRINAPGLKVKDLIGQPWTLAFALRDDGWWLRAEVIWA
                     RTNPTPEPTPDRPIKSHSTIFMLTKRARYLYHPMREPSSGTANRKGRKLATPREANGH
                     HAGWHAATADLVDERLVRDVWLVPVEGNAVEHYATWPREIARRMILASTSEGDVVLDP
                     FLGTGTTALVARQHRRRAVGVELSPAYLEQARRRLALPDVDGAVPVGGGHAQMTLLG"
     gene            2371..>2951
                     /gene="dcm"
                     /locus_tag="PLX85_05300"
     CDS             2371..>2951
                     /gene="dcm"
                     /locus_tag="PLX85_05300"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HRC62622.1"
                     /translation="MGALRAGSTFSGVGGLDLGLRRAGLDHAFFAELDPWRRAVLAAH
                     WPGVDVYDDVHAVRSGAGPVDVLAGGFPCQDVSIAGHRRGLAGARSGLFFEFARIAAD
                     LQPRWLLVENVPGLLTANGGRDFGCVLAELADLGYGVAWRHLDARYFGVAQRRRRVFL
                     VGHLGGRPELALRCLDEGSGGHLEAGVCTWQAAA"
BASE COUNT          389 a         1051 c         1080 g          431 t
ORIGIN      
        1 gggcctgctg ctgggcgcac gcgaacggcc acgtccgcct gcggacgtgg ctgtatcgcg
       61 acgtgtacct gcacacgcgg cactggtgcc gcgtcgcctc ggagacgaag cgccgggcgg
      121 ggggtgtggg gggaggggct ccgcggtgtc agcatcctgc gcatcgcggc gcggcgtcgg
      181 cgttcgatgc gcaccacgtc cgctaccggg acgagtcggg ccgctgcctc ctggggaggg
      241 agcggcccgg ggtcgacact cggtgctggt gccgcgcgtg tcacgacgcg gcgcatggcc
      301 ggcctagccg tcgtcgaggc tgagcgtgtc gggctggacg acgtcgcggc gctcgccgta
      361 gtcgacgagg acgccggtag gcgcggccgc ggggctcttg atgctctgca gctcgacggt
      421 cgcgcggcag ccgttcgtcg cgacggcgac acggtcgccg acgtagacgg gccggtggat
      481 cgtgacgcgc tcgtcggcgc ggaacgtctg cgcgcgccgc gtcgagggac gccagagctg
      541 ttcttgcacc tcgtagccgg tgacgcggac gctgccgtcg ctcatcggct cgacgtcgac
      601 gacgcggcag cggtggccgt ggatgcgggc gtgcagctcg cgggcgatga ggagcgcgcc
      661 gagcgccgcg ggatcgcggc gctcggtcgt ccagcagacg aaggtggcgg tggtcatgcg
      721 cggtcccgca gctcggccgc ggtgcactcg ctgatggccg cgatggcctc gtcgcccgcg
      781 gtcgtcttcg gggtgagcgc gtcggtggtg gtcatggcgt gacctcctcg ggtcgtttgg
      841 ggctagggat gggtcacgac gcggcgagcg cgtcggtctc gtcggcccac tcgggccagt
      901 tggcgtatgc gtcgacgggc ggcttgccgg ccttgacggc ctcggcgaac cggcgggtcg
      961 cctcgacgat cgtgtcggcg acgcggtgca gcggcacgca gtcgatcgcg agccagcggc
     1021 cgtcgagata ggcgtcctcg tcgagcgcgt agacgcacac ggtcgcctgc gagtcgttgt
     1081 cgaaatcgac gctcgggtcg ccgctgacgg tgacccacgc gtacgagtcg ccgtagccgt
     1141 cgacgtgaac ctcgaaggcc tcgcagccgc ctccggtgcc gatcaggctg gccgagtcgc
     1201 cgccggggac gatcgcggcg atttgccgcg cgaccgcgag cgtgtcgcgg ctgtacttcg
     1261 tgaggctggc gatcaggcgg gggttcgtgt ccataggggg aacatacctc ccatcgcgtc
     1321 gcgtgtcaag ccttcttaca cgtccggcga tgggggtatg atgccaccca tgcgacccga
     1381 gccctggcac gccgacaacg acgtgaccat ctaccacggc gacgcgctcg ccacgctccg
     1441 cgagctgccc gacgagtcgg tgcagatgtg cgcgacctcg ccgccgttct gggcgctgcg
     1501 cgactacggc gtcgacggcc agctcggcca ggaggcgacg ctcaaggagt gggccgccaa
     1561 cctcgtcgac gtgttccgcg aggtacgccg cgtcctgaag cccgacggca cgctctgggt
     1621 cgaggtcggc gactcgtaca acgccggcac cgccggccgc aacggcggca agagcggcgc
     1681 ggcgcgcgac cacctcaccg tcgcctactg gcgcaaggcc ggccagatgg gcgatcgccg
     1741 catcaacgcg ccgggcctga aggtgaaaga cctgatcggc cagccgtgga cgctcgcgtt
     1801 cgcgctgcgc gacgatggct ggtggctccg agcggaggtc atctgggcgc ggacgaaccc
     1861 gacgcccgag ccgacgcccg accggcccat caagagccac tccacgatct tcatgctcac
     1921 gaagcgggcc cgctacctct accacccgat gcgcgagccc tcgagcggca ccgcgaaccg
     1981 caagggccgc aagctcgcga cgccacgcga ggccaacggc caccacgccg gctggcacgc
     2041 ggcgaccgca gatctcgtcg acgagcggct cgtgcgcgac gtctggctcg tgcccgtcga
     2101 gggaaacgcc gtcgagcact acgcgacctg gccgcgcgag atcgcgcgcc gcatgatcct
     2161 cgcgtcgacc agcgagggcg acgtcgtcct cgacccgttc ctcgggacag gcacgaccgc
     2221 gctcgtcgca cgccagcatc gccgccgagc tgtcggcgtc gagctgagcc cggcgtacct
     2281 cgagcaagcg cggcgcaggc tcgcgctgcc cgacgtcgac ggggccgtcc ccgtcggcgg
     2341 cggccacgcc cagatgaccc tcctcggctg atgggagccc tccgtgcagg atcgaccttc
     2401 tccggcgtcg gcggcctcga cctcggcctc cgacgcgccg gcctcgacca cgccttcttc
     2461 gccgagctcg acccgtggcg acgcgccgtc ctcgccgccc actggcccgg cgtcgacgtc
     2521 tacgacgacg tccacgctgt tcgatccggg gccggcccgg tcgacgtcct cgcaggaggc
     2581 ttcccgtgcc aggacgtcag catcgccggc caccgtcgcg ggctcgcagg cgcgcgcagc
     2641 ggcctcttct tcgagttcgc ccgcatcgcc gccgatcttc aaccccgctg gctactcgtc
     2701 gagaacgtac ccgggctgct caccgcgaac ggcggtcggg acttcggatg cgtgcttgcc
     2761 gagctggccg acctcgggta tggcgtggcg tggcggcatc tcgacgcacg ctacttcgga
     2821 gtcgcccagc gccgccgtcg agtgttcctt gtcggccatc ttggaggccg acccgagctc
     2881 gcgcttcgat gtctcgacga ggggagcggc gggcatcttg aagcgggcgt ctgcacgtgg
     2941 caagcggctg c
//
LOCUS       DAORPG010000196         2942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3433_length_2942_cov_3.051264, whole genome shotgun sequence.
ACCESSION   DAORPG010000196 DAORPG010000000
VERSION     DAORPG010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3433_length_2942_cov_3.051264"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..2341)
                     /locus_tag="PLX85_05305"
     CDS             complement(29..2341)
                     /locus_tag="PLX85_05305"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRC62623.1"
                     /translation="MLQRLARASYQHPWRAVAGWVLILVAVFGAVGVVGAAFDNTFQI
                     PASESRRGFDALDAHFGGAGSGQSGSIVFRARQGVDDPAVRSAMEAMFAEVATYRGVQ
                     VTSPYSEQGAAQVNPDRTVAYASLSLQQDLDFNTTAQYGADMAKIRPQIDGLTVEIGG
                     GALAEFKPPESEFIGLSFAVVVLILAFGSVLAMGLPLSVAVVGVGTGIGLVSLISNLI
                     SMPDFATTIGAMIGLGVGIDYALFIVTRFREGLHAGEGPEQSTVVAMNTAGRAVIFAG
                     LTVVISLLGMLLMGLAFIAGLGIGAAVTVLTTMLASITLLPALLGFARERVEVTRWYG
                     LLAAGFVSLALFGAGLGLTALLVGFPIAVLVFIGGRWVPALKELVPKRAPGAPQRAVA
                     YRWSRMVQAHPWLAVFVGSAILIALASPMLSLRLGFSDEGNYREETTTRRAYDLLAEG
                     FGPGFNGPLLVTAEVRNDADRNALQPLVAALKGADGVASVSPPIPSDRANPNASPAYL
                     VQVVPTTAPQDAATSDLVNRLRQEVIPGALGGSTLAVNITGTVAANIDFSDYLAGRLL
                     LFFGAVLTLSFLLLMAVFRSVVVPLKAVVMNVLSIGAAYGVVIAIFQWGWFGWLFGTA
                     EAPIEPFIPMMMFAIVFGLSMDYEVFLLSRVKEEYDRSGNAFTSVADGLASTARVITA
                     AAAIMVVVFGSFMFEDDRVVKLFGVGLATAVLLDASVVRMLLVPATMELLGKSNWWLP
                     GWLDRVLPRISVEAPPQVVAAPVRVEGGDE"
     gene            2796..>2942
                     /locus_tag="PLX85_05310"
     CDS             2796..>2942
                     /locus_tag="PLX85_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HRC62624.1"
                     /translation="MTTTFTSSPSLLLTSESVTEGHPDKLCDQVSDAVLDAILQQDPM
                     ARVAC"
BASE COUNT          511 a         1186 c          849 g          396 t
ORIGIN      
        1 cccgaagcgt cagcggcgcg ccctgccgct actcgtcgcc gccctccacg cgcaccggcg
       61 ccgccaccac ctgcggcggt gcctccacgc tgatccgagg caacacccgg tccagccagc
      121 caggcagcca ccagttgctc ttccccagca gctccatcgt cgccggcacc agcagcatgc
      181 gtaccaccga ggcgtccagc agcaccgcgg tcgccagccc caccccgaac agcttcacca
      241 cccggtcgtc ttcgaacatg aagcttccga acaccaccac catgatcgcc gctgccgccg
      301 tgatcacccg cgccgtcgag gcgagaccat ccgccaccga ggtgaacgcg ttccccgagc
      361 ggtcgtactc ctccttcacc cgcgagagca ggaacacctc gtagtccatc gacagcccga
      421 acacgatcgc gaacatcatc atcgggatga acggctcgat cggcgcctcc gccgtcccga
      481 acagccagcc gaaccagccc cactggaaga tcgcgatcac caccccgtag gccgccccga
      541 tcgacagcac attcatcacc accgccttca gcggcaccac caccgagcgg aacaccgcca
      601 tcagcagcag gaacgacagc gtcagcaccg cgccgaagaa cagcagcagc cgcccggcca
      661 ggtagtcgct gaagtcgatg ttcgccgcca ccgtccccgt gatattcacc gccagcgtcg
      721 agccgccgag ggctcccggg atcacctcct gccgcaggcg gttcaccaga tcgctcgtcg
      781 ccgcatcctg cggcgccgtc gtcggcacca cctgtacgag gtacgcgggc gacgcgttcg
      841 ggttcgcccg gtcgctcggg atcggcgggg acacggaggc cacgccgtcg gcgcccttga
      901 gcgccgccac cagcggctgc agcgcgttgc ggtccgcgtc gttgcggacc tcggccgtca
      961 ccagcaacgg cccgttgaac ccgggcccga acccctccgc cagcaagtcg tacgcccgcc
     1021 gcgtcgtcgt ctcctcccgg tagttcccct cgtccgagaa cccgaggcgc agtgaaagca
     1081 tcggcgaggc cagcgcaatc aggatcgccg accccacgaa caccgcgagc caggggtgcg
     1141 cctgcaccat gcggctccag cggtacgcca ccgcccgctg cggggcgccc ggcgcgcgct
     1201 tcggcacgag ttccttcagc gccggcaccc agcgcccgcc gatgaacacc agcactgcga
     1261 tcgggaaccc gaccagcagc gccgtgagcc cgagaccggc cccgaacagc gccagcgaca
     1321 cgaaccccgc ggcgaggagc ccgtaccagc gcgtcacctc cacccgctcc cgtgcaaacc
     1381 cgagcagcgc cggcagcagc gtgatcgagg ccagcatcgt cgtcagcacg gtcaccgccg
     1441 cgccgatgcc gaggcctgcg atgaacgcca gccccatcaa cagcatcccc agcagcgaga
     1501 tcaccaccgt cagcccggca aagatcacgg cccgccccgc cgtgttcatc gccaccaccg
     1561 tcgactgctc cggcccctcg cctgcatgca gcccctcacg gaaccgcgtc acgatgaaca
     1621 gcgcgtagtc gatccccacg ccaaggccga tcatcgcccc gatcgtcgtc gcgaagtccg
     1681 gcatcgagat caggttcgaa atcagcgata cgaggccgat gcccgtaccc acgcccacca
     1741 ccgccacgct cagcggtagc cccatcgcca gcacggaccc gaatgcgagg atcaatacga
     1801 ccaccgcgaa cgacagtccg atgaactcgg actcgggcgg cttgaattcc gccagcgccc
     1861 cgcctccgat ctccaccgtc agcccgtcga tctgcggccg gatcttcgcc atgtccgcgc
     1921 cgtattgcgc cgtcgtgttg aagtcgaggt cctgctgcag gctcagcgag gcgtatgcca
     1981 ccgtccggtc cgggttcacc tgcgcggccc cctgttccga gtaggggctc gttacctgga
     2041 cgccgcggta ggtcgccacc tccgcgaaca tcgcttccat cgccgagcgc accgccgggt
     2101 catccacccc ctgccgtgct cggaacacga tcgagccgct ctggccgctg cccgcccccc
     2161 cgaagtgcgc gtcgagggca tcgaatcccc gccggctctc cgacgccggg atctggaacg
     2221 tgttgtcgaa cgccgccccc acgaccccga ccgccccgaa caccgccacg aggatgagca
     2281 cccaccccgc cactgctcgc catggatgct gatacgaggc gcgcgcaagt cgctgcagca
     2341 taggaggagg cctttctcga cagcagggaa tgaagccggt ggcggcggat aaacgaagcg
     2401 gatgatcagg ttactgcccc acgctgcgtt tcggcaccca cgctgtcacc cgcggccggg
     2461 tggatgggag agcctcgccc ccttcgaggc gtgtgcttcg ttgtctcgtg cgaccgtccc
     2521 caacgaggcc acgctggacc cgtacctgcg cggcgaaggg gggttgctgc cagtcggacg
     2581 ttcagcgcag acgattcccg gttcggtctc cggtcctggt cgcccgccat cgaacgctcc
     2641 cgaggacaac ccgcctgccg atctgctggc ggtcgaagtt gccctgaatc gcgttcgtcg
     2701 cccgcacccc cccatcgccc accgttccgg tggcgcaccg ccccgccgag gtgggaggat
     2761 agccgcacgc tcgcccgcct gactcggagg actgcatgac caccaccttc acgagctcgc
     2821 cctcgctgct actcaccagc gaatccgtca ccgagggcca tcccgacaag ctctgcgacc
     2881 aggtctctga cgcggtcctc gacgcgatcc tccagcagga ccccatggcc cgcgtcgcct
     2941 gt
//
LOCUS       DAORPG010000197         2932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3455_length_2932_cov_3.931178, whole genome shotgun sequence.
ACCESSION   DAORPG010000197 DAORPG010000000
VERSION     DAORPG010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3455_length_2932_cov_3.931178"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="PLX85_05315"
     CDS             complement(<1..171)
                     /locus_tag="PLX85_05315"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HRC62625.1"
                     /translation="MSARGDWREGLPEEARLAVADLGPVAAVRFDSREVQPGDVFVCI
                     RGERSDGHAFASA"
     gene            274..690
                     /locus_tag="PLX85_05320"
     CDS             274..690
                     /locus_tag="PLX85_05320"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRC62626.1"
                     /translation="MIDLTEEMQQAINDSLADGRPVVFASVSPDGQPRLGFVGSIHVH
                     SADQLSVWLRKADSTSAENIRNNPHLAFLYRNPDKRQTWQFQGRARIVDDAATRQRVF
                     DDAPEVERNFDQEMQGHAVVIDLDRVIERGQVIMER"
     gene            complement(702..1075)
                     /gene="rnpB"
                     /locus_tag="PLX85_05325"
     ncRNA           complement(702..1075)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLX85_05325"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1108..2361)
                     /locus_tag="PLX85_05330"
     CDS             complement(1108..2361)
                     /locus_tag="PLX85_05330"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC62627.1"
                     /translation="MTASGSATTDQTRRPGTWAALRFPQYRILWLATIAAILTSELRL
                     VVTGVWLYEETGSAAQLGVLGLIQLFVQIPALLFGGTLADRLDRRLLMAWTQGVTLLF
                     ISGLALLVLLDALEPWHVYAATAVLSVSTVFGNPARSALISATVPEEQLRDAVTTNWM
                     TQQIAIVVAPLGFAATVQTLDLRAAFVLTAIVAVPSVLLPLMLRVRGVPAQMVEGGSM
                     LRRTWDGLRFVRGHPLLPALFFMDTGVTIVSFYRQMYPVLADELFHGGAATVGLLTAA
                     NSIGAIIGSFAVLRLRHVRRLGMMVLVGHLLYAVLLFPFALVHWLPVGLLCIAGLGGT
                     DAISVTMRQATAQLTTPDEMRGRALSVMTLSAQTANNVGTLWVGMLSAAIGATGTLVV
                     GGTASLTFVLGVWQRVRGLREYEAP"
     gene            complement(2358..>2932)
                     /locus_tag="PLX85_05335"
     CDS             complement(2358..>2932)
                     /locus_tag="PLX85_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62628.1"
                     /translation="DETLRGDLRAELLRLHRELDVPVVFVTHDLREAHLLADRIAVID
                     DGKVLQYAPREEVFRHPRVRRVAELTGVRNLFGGCLDHGRVVVNGLRLAAEVPAGLGP
                     EVDVAIRAERCNLRRYDPEGELPENCFVAHVVTDLAFGNTHTLHLEPESAGPSVEVEV
                     ASRPYEVLGVASRGRWVVELPAPDLHIMPR"
BASE COUNT          507 a         1011 c          997 g          417 t
ORIGIN      
        1 cgcggaagcg aacgcatgcc cgtccgatcg ctcgccgcgg atgcagacga acacgtcacc
       61 tggctgcact tcacgcgagt cgaagcgcac cgctgcaacg ggtccaaggt cggccacggc
      121 cagccgtgcc tcctcgggca gtccttctcg ccagtcgccg cgagcactca tcgcgcccat
      181 cgtagcgcgt ccccgcctct acgccgcgac cggacgcacg atgtaggatg taccccacca
      241 ccgaggggta tccgacgagg gagcagcgcg aacgtgatcg atctgaccga ggagatgcag
      301 caggccatca atgactcgct ggccgacgga cgccccgtcg tcttcgccag cgtttcgcct
      361 gacggccaac cgcggctggg cttcgtcggc tcgatccacg tgcacagcgc ggaccagctc
      421 tcggtctggc tgcgtaaggc cgacagcaca tcggccgaga acatccgcaa caacccccac
      481 ctggcgttcc tgtaccgcaa cccggacaag cgccagacat ggcagttcca gggtcgcgcg
      541 cgcatcgtgg acgacgcggc aacgcgccag cgcgtcttcg atgacgcccc ggaggtggag
      601 cgcaacttcg accaggagat gcagggtcac gcggtcgtga tcgacctcga ccgggtgatc
      661 gagcgtggcc aggtgatcat ggagcgctga gcgagcggac gtaggccagg gggcctgtaa
      721 gccgaattct gtaccgccca cgagggcggc gacggccatc catctaggac gacggttacc
      781 cgtcgcctcc agcagcctac ccgggagcag ggccggacac cctgcgcgct cccctatttg
      841 gccttgcttc gggtggggtt tgcccggccg ccctgtcacc aggacgccgg tgcgctctta
      901 ccgcaccatt tcacccttac ctcgccgcct gggaggcggc gaggcggttt gtttctgttg
      961 cactgtccgt cggctcgcgc cgcccggccg ttagccggca ccctgtccgg tgaagttcgg
     1021 actttcctcc cgcggcggcc tcgcggcctc cgccggcggc cgtccggccc cctggcaacg
     1081 tcattgtacc gccggaggcg gcgaggctca gggcgcctcg tactctcgga gcccgcggac
     1141 gcgttgccac acgccgagga cgaacgtgag cgaggcggtg ccgccgacga ccagcgtccc
     1201 tgtcgccccg atggcggcgg agagcatgcc gacccagagc gtgccgacgt tgttcgcggt
     1261 ctgcgcggag agcgtcatga cgctcagcgc tcgtccgcgc atctcgtcgg gcgtggtgag
     1321 ctgcgccgtc gcctgacgca tcgtcacgct gatggcatcc gtgccgccga ggcccgcgat
     1381 gcagagcaga ccgaccggta gccagtgcac aagggcgaac gggaacagca gcaccgcgta
     1441 gagcaggtgg ccgacgagca ccatcatgcc gagacgtcgc acgtgccgca gtcgcaggac
     1501 cgcgaaactg ccgatgatgg cgccaatcga gttcgccgcc gtgagcagac cgacggtcgc
     1561 agcgcccccg tgaaagagct cgtcggcgag gacggggtac atctgccggt agaagctcac
     1621 gatggtgacc ccggtgtcca tgaagaacag cgcgggcagc aggggatgtc cgcgcacgaa
     1681 gcgcaacccg tcccaggtgc gtcgcagcat cgagccgccc tcgaccatct gcgctggtac
     1741 gccccgcacc cgtagcatca gcggcagcag gacgctgggg acggcgacga tggctgtcag
     1801 tacgaatgca gcccgcaggt ccagcgtctg cacggtagcg gcgaagccga gcggcgcaac
     1861 gacgatcgcg atctgctgcg tcatccagtt cgtcgtgacg gcgtcgcgca gttgttcctc
     1921 ggggacggtc gcactgatca gcgcactgcg ggccgggttg ccgaagacgg tgctgacgga
     1981 gagcaccgcc gtcgcggcat agacgtgcca cggctcgagg gcgtcgagga gcaccagcag
     2041 cgccagaccg gagatgaaga gcaaggtcac gccctgcgtc caggccatga gcagccttcg
     2101 gtcgaggcgg tcagcgagcg tgcctccgaa cagcaacgcg gggatctgca cgaacaactg
     2161 gatcagaccg agcacaccga gctgtgcggc ggacccggtt tcctcgtaca gccagacgcc
     2221 ggtgaccacc aggcgcagtt cggaagtcag aatagccgcg atggtggcca gccagaggat
     2281 gcggtactgg ggaaagcgaa gggcggccca agtcccgggc cgcctcgtct ggtcggtcgt
     2341 cgcgctgccg ctggccgtca ccgcggcatg atatgcagat ccggcgctgg aagctcgacg
     2401 acccagcgtc cgcgggaggc gacgccgagt acctcgtaag gacgcgaggc cacctcgacc
     2461 tcgaccgacg gccctgcgga ctccggctcg aggtgcagcg tatgcgtgtt cccgaaggcg
     2521 aggtcagtga ccacgtgcgc gacgaagcag ttctcgggca gctcgccctc agggtcgtag
     2581 cggcgcaggt tgcagcgctc cgcccggatc gcgacatcga cctcgggccc aagtccggcc
     2641 ggcacctccg ccgcgagtcg caatccgttg acgacaaccc gtccgtggtc gaggcagccg
     2701 ccgaacaggt ttcgcacgcc agtcagctcc gccacgcgac ggacacgggg gtggcggaag
     2761 acttcctcgc gcggggcgta ctgcagcact ttgccgtcat cgatgaccgc gatgcggtca
     2821 gcgagcaggt gcgcctcgcg caggtcgtgg gtaacgaaga ccaccgggac gtcgagctca
     2881 cggtgcagcc gcaatagctc ggcgcgcagg tcgcctcgca acgtctcgtc ga
//
LOCUS       DAORPG010000198         2919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3476_length_2919_cov_2.863827, whole genome shotgun sequence.
ACCESSION   DAORPG010000198 DAORPG010000000
VERSION     DAORPG010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3476_length_2919_cov_2.863827"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="PLX85_05340"
     CDS             complement(<1..912)
                     /locus_tag="PLX85_05340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC62629.1"
                     /translation="MPSAASSRDSSIALLVVVALGVFVAADDQTSIVTVLPSIIRDVG
                     ITVDDFYRSSWVVNGYLLGYLVALPLLGRVADVFGRGRVFVVTLLIFILGSMLVATAP
                     SFGWLVVARAFQAVGGGGVVPVAMAIVVDELPPSRRALGLGAIAAASEAGALLGPLWG
                     GAITDLAGWRWVFWVNLPMTAPIALLGWRLTRHSGGHPGRIDWSGAMLLAAALGVFTF
                     ALVDDPLAPRPLALTVLILLVGVALGALFVRHELRTPVPMVRLSMFTIRPIAAANLAS
                     LLLGGGLIVALISVPLFVNLVLAGSPLN"
     gene            complement(944..1693)
                     /locus_tag="PLX85_05345"
     CDS             complement(944..1693)
                     /locus_tag="PLX85_05345"
                     /inference="COORDINATES: protein motif:HMM:NF018822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HRC62630.1"
                     /translation="MVGTPGKPPMTPLPIRLIASLRGLAVLLLLGAGLVAACGGGEDA
                     DPSTFPDASTLLDQSATRMEQVRSMHFLLEHDNGATQIVRGIAMNRAEGDVVTPDKMQ
                     ATVKGGLGIVNFEIGIVILGKDAWIQNPLNRRWESENITIDQVFDPRQGVIALVRATR
                     GATIAGIDDVGGVKCYRVEATLDSGNLDLLPGTPTAGKPIPTTAWIGVDDQLVRKVQF
                     RGGVSTGEPDNLVRTLTLSRFDEDVTIVPPR"
     gene            complement(1991..2815)
                     /locus_tag="PLX85_05350"
     CDS             complement(1991..2815)
                     /locus_tag="PLX85_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRC62631.1"
                     /translation="MQLDLLIDGIDFGEGPRWHDGRLWYSDFFQHAVTPAGGRERIVE
                     VPQQPSGLGWLPDGRLLVVSMLDRRLLRLEEGGHLVEHADLGAIATGPCNDMVVSATG
                     HAYIGNFGAPTVGGPPEAALAHVTPDGRVAEAARGLRFPNGSVITPDGRTLIVAETAG
                     AALTAFDIHDDGGLANSRTWAALDAHPDGIALDAAGAIWLADPGGRRVVRVLEGGEVT
                     HALDTEDGAYACALGGPDRRTLYILTAPGFTAEDCAGKHAGRILHTRVDIPGAGVP"
BASE COUNT          471 a          830 c         1204 g          414 t
ORIGIN      
        1 attgagcggg gagcccgcga ggaccagatt cacgaagagg ggcacgctga tcaaggccac
       61 gatcagcccc ccgccgagga gcaggctggc gaggttggcg gcggcgatcg gacggatggt
      121 gaacatcgat aggcggacca tcgggacggg ggtgcgcagc tcgtggcgaa cgaacagcgc
      181 gccgagcgcg acgcccacga gcaggatgag gacggtgagc gccaacggcc tcggggcgag
      241 gggatcgtcg acgagggcga aggtgaacac accgagcgcc gccgccagca gcatcgcgcc
      301 cgaccaatcg atgcggccgg gatgccctcc ggagtggcgg gtcaggcgcc agccgaggag
      361 agcgattggc gcagtcatcg gcaggttcac ccagaacacc caacgccagc cggccagatc
      421 cgtgatggcg ccgccccaga gcggaccgag cagggcgcca gcctcggagg ccgcggcgat
      481 ggcaccgagg cccagcgcac gcctcgatgg tgggagctcg tcgacgacga tggccatggc
      541 aacgggaacg acaccaccgc cgcctacggc ctgaaatgcc cgcgcgacga cgagccagcc
      601 gaagctcggc gcggtggcca cgagcatgct gccgaggatg aagatgagca gggttacgac
      661 gaagacgcgg ccgcggccga acacgtcggc aacgcgtccc agcaggggga gggcgacgag
      721 gtagccgagc aaatagccgt tcacgaccca ggaggagcga tagaagtcgt ccaccgtgat
      781 gccgacgtca cggatgatcg aggggagcac cgtcacgatc gacgtctggt cgtcggcggc
      841 gacgaagacg ccaagggcga cgacaaccaa gagtgcgatg gaggagtcgc ggctcgatgc
      901 cgcggagggc atcgaagcgg cgtcagccgg tgggggcggg ccgctagcgc ggcgggacaa
      961 tcgtgacgtc ctcgtcaaag cggctgaggg tgagcgtgcg gacgaggttg tcgggctcgc
     1021 cagtgctgac gccacctcgg aactgcacct tgcggacgag ttggtcgtcg acgccgatcc
     1081 aggcggtggt cgggatgggc ttgccggcgg tcggtgtgcc gggcaggagg tcgaggttgc
     1141 cggagtcgag ggtggcttcg acgcggtagc acttcacgcc gccgacgtcg tcgatgccgg
     1201 cgatggtcgc gcctcgagtc gcgcgcacga gggcgatgac gccctggcgg gggtcgaaga
     1261 cctggtcgat ggtgatgttc tcggactccc agcgccggtt gagcgggttc tggatccagg
     1321 cgtccttgcc gaggatgacg atgccgatct cgaagttgac gatgcccagt ccgcctttga
     1381 cggtggcctg catcttgtcg ggggtgacga cgtcgccctc ggcgcggttc atggcgatgc
     1441 ctcggacgat ctgggtggcg ccgttgtcgt gctcgaggag gaagtgcatg gagcggactt
     1501 gctccatgcg ggtagcggac tggtcgagga gcgtggaggc gtcagggaag gtgctgggat
     1561 ccgcgtcctc gccgcctccg caggcggcta cgaggcccgc accgaggagg aggagcacgg
     1621 cgagcccgcg gagggatgcg atcaggcgga tggggagtgg ggtcatcggc ggctttccgg
     1681 gtgtgccaac catcctcgca ctcctcggaa gggggcagca acgcggattc gtttccgggc
     1741 tgcggatcga aacgaaggaa acgaatcgcc tcgctcagat tcgatccgtg attcgtttcc
     1801 ttcgtttcat tcgttttcga ggtgttttgc gctggccgga ggggtccggg cggagcgata
     1861 ttccgagggc gtgcgttgat tcccgtggca tgcggctgtc ctccgtgcgc ggtcagggta
     1921 cggcgatcgg cgggcgaagg ccgatacgca agtagcccgg gccgttcgcc aacgggtagc
     1981 gcgagggggg ctacgggaca ccggcgcccg ggatgtcgac gcgggtgtgg aggatgcggc
     2041 cggcgtgttt gccggcgcag tcttcggcgg tgaaaccggg agcggtgagg atgtagaggg
     2101 tgcggcggtc ggggccgccc agggcgcagg cgtaggcgcc gtcctcggtg tcgagggcgt
     2161 gggtgacttc gccgccttcg aggacgcgga cgacgcgccg gcctccgggg tcggcgagcc
     2221 agatggcgcc agcggcgtcg agggcgatgc cgtcggggtg ggcgtcgagg gcggcccagg
     2281 tgcgggagtt ggcgaggcca ccgtcgtcgt ggatgtcgaa ggcggtgagg gcggcgccgg
     2341 cggtctcagc gacgatgagg gtgcgaccgt cgggggtgat gacagatccg ttggggaagc
     2401 ggaggccgcg ggcggcctcg gcaacgcggc cgtcgggggt gacatgggcg agggcggcct
     2461 cgggcgggcc gcctacggtg ggcgccccga agttgccgat gtaggcgtgg ccggtggcgc
     2521 tgacgaccat gtcgttgcag gggccggtgg cgatggcacc gaggtccgcg tgttcgacga
     2581 ggtggccgcc ttcctcgagg cggaggaggc ggcggtcgag catggagacg acgaggaggc
     2641 ggccatccgg gagccagccg aggccggagg gttgttgagg cacctcgacg atccgttcgc
     2701 ggccacctgc gggggtgacg gcgtgctgga agaagtcgga gtaccagagg cggccgtcgt
     2761 gccaacgggg gccttcgccg aagtcgatgc cgtcgatgag gaggtcgagt tgcatagggg
     2821 cggcctcgta gggtgggact cctcggagcc cggctgggat tacgggcgat gataggggcg
     2881 cgcggggcgg ggcgggggtg cctggtagct cggacggtt
//
LOCUS       DAORPG010000199         2914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3485_length_2914_cov_3.753061, whole genome shotgun sequence.
ACCESSION   DAORPG010000199 DAORPG010000000
VERSION     DAORPG010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3485_length_2914_cov_3.753061"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /gene="truB"
                     /locus_tag="PLX85_05355"
     CDS             complement(<1..140)
                     /gene="truB"
                     /locus_tag="PLX85_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521249.1"
                     /note="catalyzes isomerization of specific uridines in RNA
                     to pseudouridine; responsible for residues in T loops of
                     many tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRC62632.1"
                     /translation="MRGFLNIDKPEGMTSFDVVRAVRRAARVKRVGHAGTLDPLATGV
                     LPV"
     gene            complement(165..527)
                     /gene="rbfA"
                     /locus_tag="PLX85_05360"
     CDS             complement(165..527)
                     /gene="rbfA"
                     /locus_tag="PLX85_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRC62633.1"
                     /translation="MTRRMEKVADLLQSEIAELLLREVKHPALSDVMLSITHVEVSPD
                     LKWARVHVSLMGDQERMPEVLAALARTEPFLHRNLVKRLHMRTVPRLWFHPDHSIQEG
                     DRLTSIMREVAESEGREL"
     gene            complement(577..2367)
                     /gene="infB"
                     /locus_tag="PLX85_05365"
     CDS             complement(577..2367)
                     /gene="infB"
                     /locus_tag="PLX85_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRC62634.1"
                     /translation="MTQAEQRVRIPQAVIVKDLADLLGVTPIDVIKELMKNGVMATIN
                     QVVDFDTAAVVAADLGFSPEEEGAAPAAAAEAAEEAAAPESTSRRIMDESADAVTRPP
                     VVTVLGHVDHGKTTLLDSIRRANVAGGEAGGITQHIGAYQAAAPDGRTVTFIDTPGHA
                     AFSEMRARGAQVTDVAIVVIAADDGLMPQSREAIDHVRAASVPLVIALNKIDANNANP
                     DRVKQQLMEVGLVPEEYGGDTVVVPVSALRGEGINDLLENVLLVADLQELRANPKRDA
                     VGVVLEASTDRHRGVVATVLVQTGTLHVGDTIIAGLAYGKVKAMNDENGQRVKSAGPS
                     TPVMVLGLSEVPPAGERVTVLKDEKEARAEAETRRRRLEEAGTQTDGVTLESLFGEIH
                     RGAVRDFNIVLKTDVQGSIEPLVRALEGASVEGVNVKVIHASAGSINESDVNLAVASK
                     GVIIGFNTSPEPGARKLAEQERVEIREYRVIYDILDDVEKAVRGLLQPVYEEAEDGRA
                     EVRQVFRLGRRNAIAGCYVREGNIRRNSLARVFRGGELVHESRIDSLKRFQEDAREVA
                     SGFECGIQVDGFSDFQEGDEVIAYHMEQTR"
     gene            complement(2364..2750)
                     /locus_tag="PLX85_05370"
     CDS             complement(2364..2750)
                     /locus_tag="PLX85_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HRC62635.1"
                     /translation="MATPPSPRSGTPRPGKVRRQPQRTCVACRDTSDKRALTRLVRGP
                     DGHVHLDRTGRANGRGAYLCDRPQCWERALGRADVLGRALKATLSAEDRAAIREEAPS
                     LEAADAHDDTNEHKDATADERAGEDA"
BASE COUNT          366 a         1076 c          971 g          501 t
ORIGIN      
        1 acgggcagca cgcccgtggc gagcgggtcg agcgtgccag cgtgcccgac gcgcttcacg
       61 cgcgcggcgc gtcgcaccgc ccgcacgacg tcgaacgagg tcatgccctc gggcttgtcg
      121 atgttcagga agccgcgcat cgaggtatca gaccggccca gccgctacag ctcgcggcct
      181 tccgactccg cgacctcgcg catgatgctc gtcaggcggt cgccttcctg gatcgaatgg
      241 tcggggtgga accagagccg aggcaccgtc cgcatgtgca gccgcttcac gaggttccgg
      301 tgcaggaacg gctccgtccg cgcgagcgcc gcgagcacct cgggcatgcg ctcctggtct
      361 cccatcaggc tgacgtgcac gcgcgcccac ttgaggtccg gcgatacctc gacgtgcgtg
      421 atcgagagca tgacgtccga cagcgcgggg tgcttcacct cgcgcagcag cagctcggca
      481 atctccgact gcagcaggtc cgcgaccttt tccatccgac gcgtcatcga ggtgtcctcg
      541 gtagtccccg cgatcgtccg gccggagcag acgcgactag cgcgtctgct ccatgtggta
      601 cgcgatgacc tcgtcgcctt cctggaagtc gctgaagcca tcgacctgga tgccgcactc
      661 gaagccggag gccacttcgc gggcgtcctc ctggaagcgc ttcaggctgt cgatgcgcga
      721 ctcgtgcacc agctcgccgc cgcggaacac ccgcgccagc gagttgcggc ggatgttgcc
      781 ctcgcgcacg tagcagcccg cgatcgcgtt ccgccgtccg aggcggaaca cctgccgcac
      841 ctcggcgcgc ccgtcctcag cctcctcgta caccggctgc agcaggccgc ggacggcctt
      901 ctcgacgtcg tcgaggatgt cgtagatcac gcggtactcg cggatctcga cgcgttcctg
      961 ctcggcgagc ttgcgcgctc ccggttccgg cgaggtgttg aacccgatga tgacgccctt
     1021 cgaggcgacc gcgaggttca cgtccgactc gttgatcgag cccgccgagg cgtgaatcac
     1081 cttgacgttc acgccctcga cgctcgcgcc ctccagcgcc cggacgagcg gctcaatcga
     1141 gccctgcacg tccgtcttga gcacgatgtt gaagtcgcgc accgcgccgc ggtggatctc
     1201 gccgaacagg ctctcgaggg tgacgccatc ggtctgcgtc ccggcctcct cgagccggcg
     1261 gcggcgcgtc tcagcctccg cgcgggcttc cttctcatcc ttgagcacgg tcacgcgctc
     1321 gcccgccggc ggcacctcgc tgagcccgag gaccatgacc ggcgtcgagg ggccggcgct
     1381 cttcacgcgc tggccgttct cgtcgttcat cgccttcacc ttgccgtagg cgaggccggc
     1441 gatgatggtg tcgcccacgt gcagcgtccc cgtctgcacg agcacggtcg ccacgacgcc
     1501 gcggtggcgg tccgtcgacg cctcgaggac tacgcccacc gcgtcgcgct tcgggttggc
     1561 gcgcagctcc tgcaggtccg cgaccagcag cacgttctcg agcaggtcat tgatcccctc
     1621 tccgcgcagc gcggagaccg gcacgacgac ggtgtcgccg ccgtattcct cggggacgag
     1681 gccgacctcc atcagctgct gcttcacgcg gtcggggttg gcgttgttgg cgtcgatctt
     1741 gttgagcgcg atcacgagcg gcacgctcgc cgcccgcacg tggtcgatcg cctcgcggct
     1801 ttgcggcatc aggccgtcgt ccgccgcgat cacgacgatg gccacgtccg tgacctgcgc
     1861 gccgcgtgcc cgcatctcgc tgaacgcggc gtggcccggc gtgtcgataa acgtcacggt
     1921 gcgcccgtcg ggcgccgccg cctggtatgc accgatgtgc tgcgtgatgc cgcccgcctc
     1981 accgccggcg acgttcgcgc gccggatcga gtcgagcagc gtcgtcttcc cgtggtcgac
     2041 atgtcccagc accgtcacga cgggaggccg cgttaccgcg tccgcgctct cgtccatgat
     2101 gcgccgcgag gtgctctccg gggctgcggc ctcctccgcc gcttcggcag cggctgcggg
     2161 cgcggcgcct tcctcctccg gcgagaagcc gaggtccgcg gcgaccaccg ccgcggtgtc
     2221 gaagtcgacg acctggttaa tcgtcgccat cacgccgttc ttcatcagct ctttgatcac
     2281 gtcgatgggc gtcacgccga ggaggtcggc gaggtctttg acgatgaccg cctgggggat
     2341 gcgtactcgc tgttcggcct gggtcatgcg tcctcacctg ctctctcgtc tgcggttgcg
     2401 tccttgtgtt cgttcgtgtc gtcgtgtgcg tcggccgcct cgagggatgg cgcttcctcg
     2461 cggatcgccg cgcggtcctc ggccgagagc gtcgccttga gcgcccgccc gaggacgtcc
     2521 gcgcgcccga gcgcccgctc ccagcactgc ggccggtcgc acaggtaagc gccgcgcccg
     2581 ttcgcgcggc ccgtgcggtc gaggtgcaca tgcccgtccg gcccgcggac gagccgcgtc
     2641 agcgcgcgct tgtcgctggt gtcacggcag gcaacgcacg tccgctgcgg ctgccggcgg
     2701 accttcccgg gacgaggcgt cccggagcga gggctgggag gtgtggccaa gcgcgtacct
     2761 cattcctcgt ccgggtcttc gaagccgaag tcgaactcct cgccgtgcag ctcctggagt
     2821 ccttcctcga tctcgtcgtc gtccacctcg aagcggcggc tgcgtcgtcc cgccgcggcc
     2881 gggcgcacgc gctcaggagt cgccgggcca cgcc
//
LOCUS       DAORPG010000200         2910 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3489_length_2910_cov_3.435727, whole genome shotgun sequence.
ACCESSION   DAORPG010000200 DAORPG010000000
VERSION     DAORPG010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3489_length_2910_cov_3.435727"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..549
                     /locus_tag="PLX85_05375"
     CDS             55..549
                     /locus_tag="PLX85_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020524898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRC62636.1"
                     /translation="MPQSDGADPIELLKQDHQKVKQLFDEFEQTNNRRSQQRIIRQAL
                     LELDVHAGIEEEIFYPAYQEAAQESEEEDIVLEAEEEHHVVHLLIGELRDMPEVDERT
                     AAKFTVLAENVRHHIDEEEKEMLPAAKRLLGNDRLQELGVEMARRKRELTRELKQAAA
                     STAM"
     gene            complement(567..2063)
                     /locus_tag="PLX85_05380"
     CDS             complement(567..2063)
                     /locus_tag="PLX85_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HRC62637.1"
                     /translation="MKENERVRVLVFGASGYIGSHLVPRLVAAGHVVRAASRSGAALS
                     DRGWRDVECVSADALEPGSLDEALRDIEVAYYLVHSMASGRDFASRDRQAARNFRRAA
                     ERTGVERIVYLGGLQPSGERSEHLESRGETGEILRDGPIPVTELRAGIIVGAGGAAFE
                     VIRDLSFHLRVMTTPRWVRSRTQPIALEDLLAYLVGVLAEPQTAGGTYDVAGPDVLTY
                     EGMIRGFAEVVGRRVTILPVPVLSPRISSYWLDLVTAVPANVARPLIDGLKHDLLADD
                     AAIRALIPMRLHGYREAIEQALADERRGTVPARWQEGAFVFRAGRPDVSFYSKRAGGT
                     APCHASPEAAWQEIVSVGGQNGWPAYEALWRVRATLDRLVGGVGFRRGRRHPSEARVG
                     DAIDWWRVVAIEPGRRLTLLAEMKVPGAAVLELEVLPGASESASTIAATAYFHPAGVW
                     GLLYWLTLLPVHGPVFRRLAGKLAERAEARGGGTASSSGRADEPALTS"
     gene            complement(2102..2536)
                     /locus_tag="PLX85_05385"
     CDS             complement(2102..2536)
                     /locus_tag="PLX85_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62638.1"
                     /translation="MDDAWSEAPSERAIAAFLDDYEQEIALRQASEREFSPLSSRLRS
                     LLERWTSGEVGADETRDARARYVEALRRWQPDLEHWVAIRGSGDRLLAAVEFMTDAQW
                     LRFSALQAAEMRSGISESSLDRDQAVIRSLLPRFEASLPPES"
     gene            complement(2551..2700)
                     /locus_tag="PLX85_05390"
     CDS             complement(2551..2700)
                     /locus_tag="PLX85_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62639.1"
                     /translation="MTKMISSVTQCRRCARGRVMFDGEDLCCVQCGWRCAIEVTPAEA
                     VRLAA"
BASE COUNT          515 a         1070 c          888 g          437 t
ORIGIN      
        1 gccgaggtat tcgtcctctc atatccccaa atgctcggca gaacggagac acgtgtgccc
       61 cagtcggacg gcgccgaccc catcgaactc ctcaaacagg accaccagaa ggtgaagcag
      121 ctcttcgacg agttcgagca gacgaacaac aggcgctcgc agcaacgcat catccgccaa
      181 gctcttctcg agctcgatgt tcacgcgggc atcgaagaag agatcttcta ccctgcttat
      241 caggaggccg cccaggagtc cgaggaggaa gacatcgtcc tcgaggcaga ggaagagcac
      301 cacgtcgtgc acctgctcat cggcgagctg cgcgacatgc ccgaggtcga cgaacggacc
      361 gctgccaagt tcaccgtcct cgccgagaac gtccgccacc acatcgacga ggaagaaaaa
      421 gagatgctgc cagccgcgaa gcggctgctg ggcaatgacc gcctgcagga actcggcgtc
      481 gagatggcgc gtcgcaagcg cgagctcaca agggaactga agcaggcggc ggcttcaacc
      541 gcgatgtgaa cccggcccgt acggcgtcat gaggtcagtg cgggctcgtc cgcccggccg
      601 gaggacgagg cggttccgcc gccccgtgcc tccgctcgtt cggcgagctt ccccgccagt
      661 cgccgaaaca ccggcccgtg cactggcagc agcgtgagcc agtacagcag gccccacact
      721 cccgcggggt ggaagtacgc cgtcgccgcg atcgtggacg ctgactccga cgcgcccggc
      781 agcacttcga gttccagcac ggccgccccc ggcaccttca tctccgccag cagcgtcagc
      841 cggcgccccg gctcaatcgc gaccacccgc caccagtcga tggcgtcacc aacccgtgcc
      901 tccgaggggt gccgccgacc tctgcgaaag ccgaccccgc ccaccagacg gtcgagggtc
      961 gcgcgcacgc gccacagcgc ctcgtatgct ggccagccat tctgcccgcc gaccgacacg
     1021 atctcttgcc acgcggcctc aggggaggca tggcaggggg ccgttccccc ggcgcgcttc
     1081 gagtagaacg acacgtccgg tcgcccggcg cgaaagacga acgcgccttc ttgccaccgc
     1141 gccggcactg tcccgcggcg ttcgtccgcc agcgcctgct cgatcgcctc gcggtagccg
     1201 tgtagccgca tcgggatcag cgcgcggatg gcggcatcat ccgccagtag gtcgtgtttc
     1261 agcccgtcga tgagcggccg cgcgacgttc gcgggcaccg ccgtcaccag gtccagccag
     1321 tacgaggaga tccgagggct cagcaccggt acgggtagga tcgtcacccg ccgtccgacc
     1381 acctccgcga agccgcggat catcccctcg taggtcagca cgtccggccc cgcgacgtcg
     1441 taagttccgc cggcggtctg tggctccgcc agcaccccca cgaggtaggc cagcaggtcc
     1501 tcgagcgcga tcggctgcgt gcgcgaccgc acccagcgcg gcgtcgtcat cacacgcagg
     1561 tgaaagctca ggtcgcgaat cacctcgaac gcggcgccgc cggcgcccac gatgatgccc
     1621 gcccgcagtt ccgtgacggg gatcggcccg tcacgcagga tctcgcccgt ttcaccgcgc
     1681 gactcgaggt gctccgagcg ctctccgctc ggctgcagtc caccgaggta gacgatgcgc
     1741 tccacccccg tccgctccgc ggcccggcgg aagttccgcg ctgcctgccg gtcgcgcgac
     1801 gcgaagtctc gaccggacgc catcgaatgc accaggtagt aggcgacctc gatgtcccgc
     1861 aatgcctcgt cgagcgagcc cggctcgagt gcgtcggccg agacgcactc aacgtcccgc
     1921 catccacggt ccgacagcgc ggcgccgctc cgtgatgccg ctcgcaccac atgcccggcg
     1981 gccacgagcc gcggcacgag atggctgccg atatatccgc ttgcgccgaa gacgagtacc
     2041 cgcacgcgct cgttctcctt catgccggga gcgtcccggt atgctcggac acccctcgag
     2101 gctaactctc cggcgggagc gaggcctcga accgcggcaa cagcgaccgt atcaccgctt
     2161 gatcacggtc gaggctgctc tcggagatcc cggagcgcat ctcggccgcc tgcagggcgc
     2221 tgaaccgcag ccactgtgca tccgtcatga attccacggc tgccagcagt cggtcgcccg
     2281 acccgcggat cgccacccag tgttcgaggt ccggctgcca tcgtcgcaat gcctcgacgt
     2341 agcgcgctcg ggcgtcgcga gtctcgtctg cccctacctc accgctggtc caccgctcga
     2401 ggaggctgcg caaccttgag gacagagggc tgaactcgcg ttcactcgcc tgccgaaggg
     2461 cgatctcctg ctcgtagtcg tcgaggaacg cggcaatcgc tcgttcactc ggcgcttcgg
     2521 accacgcgtc gtccaccgtg ttcctcagcg ctacgcggcg aggcgcacgg cctcagccgg
     2581 cgtgacttcg atcgcgcagc gccagccgca ctgcacacag cacaggtctt caccgtcgaa
     2641 catcacgcgt cctcgcgcac accgccggca ctgcgtcacg ctcgaaatca tcttggtcat
     2701 cggtagcctc cgtcgtgaag tcggtatgtt gtgcatctct ttgccaacca cagctcgaat
     2761 atataccgaa gtgaccaatg tagtcaatcg agttgaccaa gacatgatca aagagtgctg
     2821 aggacgtgct ccgagccgat tggtcgagcc acgttcctcc cccgtcgctc ccgggagagt
     2881 cgaccgacgc gagcagagtg cattagacac
//
LOCUS       DAORPG010000201         2905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3501_length_2905_cov_3.695439, whole genome shotgun sequence.
ACCESSION   DAORPG010000201 DAORPG010000000
VERSION     DAORPG010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3501_length_2905_cov_3.695439"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..987)
                     /locus_tag="PLX85_05395"
     CDS             complement(22..987)
                     /locus_tag="PLX85_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62640.1"
                     /translation="MRRGAPFAVAIVLLALLPAVAAAQRPEIVARAAVDRTDVGLGER
                     VRITITAQHPDSVLIDVLPPEDSSTLRVVEKPPPTTTPGKRGGPSVTQFEFVVAAFSL
                     GPQQLPPMRLTWIDDNGVSGEQAVEAPAFTVASTVAPGDTELRPLKPQLSIEGAPVAW
                     QLPAAIGGALLVLAIGVGGGLLARHRRPTAIRVAAPEPALTMEDAARRRLETMAAAHP
                     LAAGDYDAYYGTISGVVRGYLEERFAFRATALTTPELERRMTGQGVERWQARLVGGLL
                     DRCDAAVYAGRRPDPASADHDLTVAFEIIELSRPQAEPASAEALP"
     gene            complement(984..1916)
                     /locus_tag="PLX85_05400"
     CDS             complement(984..1916)
                     /locus_tag="PLX85_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRC62641.1"
                     /translation="MAAVTAADASAAASVLAAEPTSPELLAQVRRIEIRARRLASTLL
                     MGDYRSVFRGSGIEFAEAREYVVGDDVRLIDWNVTARMGTPWVKEYVEERELSVVCAV
                     DVSASQLVARSPAGRLGAAAELCALLSFAAVFHHDRAGLLTFTDRVERFVAPAHGSRH
                     VLRIVREVLHHQPTRPGTSIAAAADYLARVLPRRSIVVLISDFYDRGYEQSLRTLARR
                     HEVLALTLTDPLDLELPDLGLVEVEDAERGERMLIDTSDRGLRRRYSEAAQARVRERR
                     AALASAGVDEVPLHLDQDLVRPLIAYFRGKAARR"
     gene            complement(1919..2845)
                     /locus_tag="PLX85_05405"
     CDS             complement(1919..2845)
                     /locus_tag="PLX85_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC62642.1"
                     /translation="MKAQVARVIVGQEAMIDRLLIALLTNNHALVEGVPGLAKTLTVS
                     TLAQTLDCSFARIQFTPDLLPADVTGTLIFNPQESSFHVRRGPLFANIVLADEINRAP
                     AKVQSALLEAMQERQVTIGGSTYDLPKPFLVLATQNPIEHEGTYPLPEAQLDRFMLKI
                     QVHYPERDDERDILRRALDGHDETAEAVTDAAGVLDLMQAVQEVEVNARLREYVVRLL
                     EATRDPARYQLADLASLIEYGASPRAGVFLARAAQAYAFIHGRGYVTPDDVKTLAPDV
                     LRHRLVTTYEAEAQSISAEQIVARLLEGVGIP"
BASE COUNT          432 a          942 c         1108 g          423 t
ORIGIN      
        1 ggagccaggg cgtaccgaag ctcacgggag tgcctcggca gaggccggct cggcttgcgg
       61 acgcgacagc tcgatgatct cgaaggcgac cgtgaggtcg tgatccgcgg aggcggggtc
      121 gggccgccgc ccggcgtaga ccgcggcgtc gcaacggtcg aggaggcccc cgacgaggcg
      181 ggcctgccag cgttcgacgc cctggccggt catgcgacgc tcgagctccg gggttgtcag
      241 cgccgtggcc cgaaacgcaa agcgctcctc gaggtagccg cgaacgacgc cggagatggt
      301 cccgtagtac gcgtcgtagt cgccggccgc gagcggatga gcagcggcca tggtctcaag
      361 acgccttcgc gcggcgtcct ccatcgtgag cgccggctcg ggagcggcca cgcggatcgc
      421 tgtgggccgg cgatggcgcg ccagcaagcc tcccccgacg ccaatggcga gcacgaggag
      481 cgctccgccg atcgccgcgg gcagctgcca cgcgacgggc gcgccctcga ttgagagctg
      541 tggcttcagt ggccgcagct cggtgtcgcc gggcgccacc gtagaggcga cagtgaacgc
      601 gggcgcctcg actgcctgtt ctccgctcac gccgttgtca tcgatccagg tgaggcgcat
      661 tggtggcagc tgctgcggac cgaggctgaa cgcggccacg acgaactcaa actgcgtaac
      721 ggatggtccg cctcgcttgc ccggggtcgt cgtcgggggc ggcttctcga cgacgcgcag
      781 ggtgctcgag tcctccggcg gcaggacgtc aatcagcacg ctgtccgggt gctgcgccgt
      841 gatcgtgatg cggacgcgtt cgcccaggcc aacatccgtc cggtcgacgg ccgcgcgggc
      901 gacgatctca ggccgctgcg ctgccgcgac ggcggggagc agcgcgagga gcacaatcgc
      961 gaccgcgaac ggagcgccgc gtctcatcgg cgggccgcct tgcctcggaa gtaggcaatg
     1021 agggggcgca ccaggtcctg gtcgaggtgc agcggcacct cgtcgacgcc ggcgctcgcg
     1081 agagcagcac gccgttcgcg cacgcgagcc tgcgcagctt ccgagtagcg gcggcggagg
     1141 ccgcggtcgc tcgtgtcgat cagcatgcgc tcaccgcgct cggcatcctc gacctcgacc
     1201 aggccgaggt cgggaagttc gaggtcgagc gggtcggtga gcgtgagcgc gagcacctca
     1261 tggcggcggg cgagggtacg taacgactgc tcgtagccgc ggtcgtagaa gtctgagatg
     1321 agcaccacga tggaccgacg cgggagcaca cgcgcgagat agtccgccgc cgcggcgatc
     1381 gaggtgccgg gacgcgtggg ctggtggtgc agcacctcgc gcacgatgcg caggacatga
     1441 cggctgccgt gagcgggggc aacgaagcgc tcgacgcggt cggtgaacgt gagcagaccg
     1501 gcccggtcgt ggtggaagac cgccgcgaac gagagcagtg cgcacagctc ggcggccgcg
     1561 ccgagacggc cggcgggcga gcgggcgacg agttgcgacg cggacacgtc gaccgcgcac
     1621 accaccgaca gctcgcgttc ctcgacatac tccttcaccc agggcgtgcc catgcgcgcg
     1681 gtcacgttcc agtcgatcag gcgtacgtcg tcgccgacca cgtactcgcg cgcctcggcg
     1741 aactcgatgc ccgagcctcg gaagacgctg cggtagtcgc ccatgagcag agtgctggcg
     1801 aggcggcgcg cgcgtatctc gatgcggcgg acctgcgcga gcagttccgg ggacgtgggt
     1861 tcggcggcga ggacggatgc ggcggcggag gcgtcggccg cggtgacggc cgccatcgtt
     1921 aggggatccc gacgccttcg aggaggcgcg ccacgatctg ctcggccgat atcgactgcg
     1981 cctcggcctc gtaggtcgtc acgaggcggt gacggagcac gtcgggggcg agtgtcttca
     2041 cgtcgtccgg ggtgacgtag ccgcggccgt ggatgaaggc gtacgcctgc gcggcgcgcg
     2101 cgaggaacac gcccgctcgc ggcgaggcgc cgtactcgat caacgaggcg aggtccgcga
     2161 gctggtaacg ggcggggtcg cgcgttgcct cgagcaggcg gacgacgtac tcacgcaggc
     2221 gtgcattcac ctcgacctcc tgcaccgcct gcatcaggtc gagtacgccc gcggcgtcgg
     2281 tgactgcctc tgcggtctcg tcgtgcccgt cgagtgctcg gcgcaggatg tcgcgctcgt
     2341 cgtcgcgctc ggggtagtgt acctggatct tgagcatgaa gcggtcgagc tgcgcctcgg
     2401 gcagcgggta cgtgccttcg tgctcgatcg ggttctgggt ggcgagcacg aggaacggct
     2461 tcggcaggtc gtaggtggag ccgccgattg tgacctgacg ctcctgcatc gcctcgagga
     2521 gcgccgactg caccttggcg ggcgcgcggt tgatctcgtc tgcaagcacg atgttggcga
     2581 agagcggccc acgccggaca tgaaagctgc tctcctgcgg attgaagatc agtgtgccgg
     2641 tgacgtcagc gggcagcagg tcgggcgtga actggatgcg ggcgaagctg cagtcgaggg
     2701 tctgcgcgag cgtcgacacc gtgagcgtct tcgcgaggcc ggggacgccc tcgacgagtg
     2761 cgtggttgtt cgtgaggagc gcgatcagga ggcggtcgat catcgcttcc tggccgacga
     2821 tgacgcgcgc gacctgcgcc ttgatgcgcg cgaccgtgtc ggcgtgccgg ggctcgaccg
     2881 aaggcggccc cgccgtcgcc tgttc
//
LOCUS       DAORPG010000202         2903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3506_length_2903_cov_4.437149, whole genome shotgun sequence.
ACCESSION   DAORPG010000202 DAORPG010000000
VERSION     DAORPG010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3506_length_2903_cov_4.437149"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLX85_05410"
     CDS             complement(<1..184)
                     /locus_tag="PLX85_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62643.1"
                     /translation="MAVNVTETQAPEVPAAPIPVPEIPAPALPTEHPNEAPRLTPVEY
                     RPGRALYKTVLPGVIQV"
     gene            complement(175..549)
                     /locus_tag="PLX85_05415"
     CDS             complement(175..549)
                     /locus_tag="PLX85_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015109697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRC62644.1"
                     /translation="MDNLDQLFTDYLAVLIATVMGFVLIITALVASRLLAPYSEEREK
                     GTTYECGMLPIRRAATNVGMRFYLYAILFIIFDVEAVFIFPWALSYVGIGPMAYYEML
                     VFVAILAAGLAYAWKKGALKWQ"
     gene            complement(724..1935)
                     /locus_tag="PLX85_05420"
     CDS             complement(724..1935)
                     /locus_tag="PLX85_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62645.1"
                     /translation="MSQLRLGGLVLLFAALVACSGAATPGEVNTGQTAAATSSPEAAA
                     RTQPGQPTPSPREIRGLFTDPRPSSAALHRHLGPRPAETFSVRNQPDVVLYDIERRTE
                     TDLGPGRLPVFTADGKYLVWASQAGSAQAFEVEGGVQLDLGIRGLPRALTGDRVWVQA
                     DDENYEVAIGTGIRRALPAAPAEGVRPRPQDAGRWRLVRVYPPPPDAREPLFNLVDGS
                     GVLGAARFEARSAVLTPDGRLFLATPPVQLDDQAGVIRGLVGWSNVFEVNPETLTASF
                     IASARISQALFPFAANERYVAWTEDFCNIASFESLGRVSSFPDSNGRTRIYDRQSGTL
                     TELDQGLLVDGLTPRGELAIGAFGADALLDLETFAYTVVFPSEDRVWSPDYRYAAVGA
                     AGGHGGYCGAE"
     gene            complement(1977..2534)
                     /locus_tag="PLX85_05425"
     CDS             complement(1977..2534)
                     /locus_tag="PLX85_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62646.1"
                     /translation="MDSSQRAIARLIMWRNPGAIDGSIPHAPRTFRTHSQLGAFGEVG
                     HIGVSDLLEAVALNPQPLPPRERYAFAVASALIETVMSYAQLSEVLGAAVANNAERFA
                     IQAVEDFEELCPRLPKWPFSFKFGTWPPPPPPPPDPYDEMSFSELMVVASNLLVAAET
                     LGAGSIAEALDRSGTRIMEQALARA"
BASE COUNT          489 a          973 c          998 g          443 t
ORIGIN      
        1 gcacctggat gacgccgggc agcaccgtct tgtagagcgc gcggcccggt cggtactcga
       61 ccggcgtcag gcgcggcgcc tcgttcgggt gctccgtcgg cagcgccgga gcgggaatct
      121 cggggacagg aatcggcgct gcggggacct cgggcgcctg tgtttcggtg acgttcactg
      181 ccacttcaac gctcccttct tccacgcgta ggcgagcccc gccgcgagga tggccacgaa
      241 gacgagcatc tcgtagtagg ccatcggccc gataccgacg taggacagcg cccacgggaa
      301 gatgaagacg gcctccacat cgaaaatgat gaagaggatc gcgtacagat agaaccgcat
      361 gcccacgttc gtcgccgcgc ggcggatggg caacatgccg cattcgtagg tcgtgccctt
      421 ctcgcgctct tcggagtagg gagcgagcag ccgcgatgca acgagggcgg tgatgataag
      481 aacgaagccc atgacggtcg cgatgagcac cgccaggtag tctgtaaaga gctggtcgag
      541 gttgtccaag acactcgcac tttcagcggt cacgccacat gacgaaccgg gcctcggacg
      601 ccgctcgtcg tcagccgggg atgaccgtaa cagtggcctt agacagtgtc aacgaatcgt
      661 gaaacggcgc acgggatctt gtaaggggac tcctccccgc ggccctcgcg aggtcgcccg
      721 gactcactct gcgccgcagt agcccccgtg tccgcccgcg gcgccaacgg ccgcgtagcg
      781 gtagtccggc gaccatacgc ggtcctcact cggaaacacg accgtgtacg cgaacgtctc
      841 gaggtcgagc agcgcgtccg ccccaaacgc gccgatcgcc agctcccctc gaggcgtcag
      901 gccgtccacg aggagcccct ggtcgagctc ggtcagcgtg ccggactggc ggtcgtagat
      961 gcgcgttcgg ccgttggagt cggggaagct ggagacccgg cccagactct cgaagctggc
     1021 gatgttgcag aagtcctcgg tccacgcgac gtagcgctca ttcgcggcga acggaaacag
     1081 cgcctgcgag atgcgcgcgc ttgcgatgaa cgaagcggtg agcgtctcgg gattgacctc
     1141 gaacacgttc gaccagccga ccaggccacg gatgacgccg gcctggtcgt cgagctgaac
     1201 gggaggcgtc gcgaggaaca gacgcccgtc gggcgtgagg acggcggaac gcgcttcgaa
     1261 ccgtgccgct ccaaggacgc cggaaccgtc gacgaggtta aacagcggct cgcgagcatc
     1321 cggcggaggc ggatacacgc gaacgaggcg ccatcgcccg gcgtcctgcg gccgaggcct
     1381 tacgccttcg gcgggcgcgg ccggcagcgc gcggcggatg ccggtcccga tcgctacttc
     1441 gtagttctcg tcgtcggcct gcacccacac gcggtcaccg gtgagcgcgc gtgggaggcc
     1501 gcgaatgccg agatccagtt gcacgcctcc ttcgacctcg aaggcttgcg cgctgccggc
     1561 ctgcgacgcc cacaccaggt acttcccatc cgccgtaaac accggcagcc gccccggccc
     1621 cagatcggtc tcggtgcggc gctcgatgtc gtagaggacc acgtccggct ggttccggac
     1681 gctgaaagtc tcggcgggcc ggggcccaag atgccggtgg agtgccgccg aggacggccg
     1741 tggatcggtg aacagtcctc ggatctcgcg tggggacggg gtgggctgcc ccggctgggt
     1801 acgggcggca gcttcgggac tggatgtggc ggcggctgtt tgcccggtgt tcacctcacc
     1861 cggcgtggcg gcgccgctgc acgccacaag cgcagcgaag agcagcacca gtccaccgag
     1921 gcgcagctgg ctcacctccg aggaacgtcc agcgttcctc ggaggttccg tacgcttcac
     1981 gctcgggcga gcgcctgctc catgatccgg gtgcccgacc ggtcgagcgc ctcggcgatg
     2041 ctgccggcgc ccagcgtttc cgcggcgacg agcaggttcg acgcgaccac catcagttcg
     2101 gagaagctca tctcgtcgta ggggtccggc ggaggcggcg gcgggggcgg ccacgtccca
     2161 aacttgaagg agaacggcca cttcggcagg cgcgggcaca gctcctcgaa gtcctcgacc
     2221 gcctggatgg cgaaccgctc ggcgttgttc gccacggcgg ccccgaggac ttcgctgagc
     2281 tgcgcgtagc tcatgacggt ttcgatgagc gcgctcgcga ctgcgaacgc gtaccgctcg
     2341 cgcggcggaa gcggctgggg gttcagcgcg accgcctcga ggaggtcact cactccgatg
     2401 tgcccgactt ccccgaacgc ccccaactgc gagtgggtcc ggaacgtccg cggggcatgc
     2461 ggaatgctcc catcgatggc gcccgggttg cgccacatga tgagccgggc gatcgccctc
     2521 tggctcgaat ccatcacgat cctcctgtct gaactcgttg tgagcagacg ctacccgcgg
     2581 tggatcgagc cgtatgtaat cccgttcaca ctcaccgaac gacagacgac aacgaaggcc
     2641 gggcgtatgc ccggccttct gaatgaatcg tgacacggcg tgagggccag accccggagg
     2701 cttggcctcc cacgccgagg gctgccagga gtcggaacgg ctaactgctc gcgtcggccg
     2761 agggcaacac aacgttcgcc tcgcgctcct cgggctcttc gtcctggctg gaggagccag
     2821 cgacgaccgc agcgagacgg gcggcagccg cttcctcggc ggcctcctgc ttctcgcgga
     2881 cgctgttgta gcagttcgag cag
//
LOCUS       DAORPG010000203         2876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3569_length_2876_cov_3.500177, whole genome shotgun sequence.
ACCESSION   DAORPG010000203 DAORPG010000000
VERSION     DAORPG010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3569_length_2876_cov_3.500177"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PLX85_05430"
     CDS             complement(<1..678)
                     /locus_tag="PLX85_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62647.1"
                     /translation="MVTRTPRGRSLALEVQADLAERPDGLPALRAYAFTSGGRLIASE
                     ALNASGATRLPIPTRSEPDTMRLLIGPELEGEEPDAGELLRRGAFEERVALRPNIERL
                     NPLHVRLGAPDVARLMLRSCLVRGKLVKRVGSGGVTLTLPVRGATIDIYEVDPWHLIL
                     PKIPDLQISRLREVIDGPWPPEEMVLPPITANPADAIHPSAFLGVAGAQVETRANAGW
                     DLGAPMAA"
     gene            complement(821..2071)
                     /locus_tag="PLX85_05435"
     CDS             complement(821..2071)
                     /locus_tag="PLX85_05435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC62648.1"
                     /translation="MSQAAPHRSPSFEVTRNVRLFYVLGFLRELSPLLVIWVVYLTDY
                     RHLTLAQVGLMEGLFWLIKLGAEIPSGAFADRYGRRLTFVTGLVLEASGVVVFALAAG
                     FGLLMVSYGIWAAGLAFRSGNEEAYLYDALAVGQRQAEFGDRIGVYWALSISAVLIGG
                     LLGGLLADLAGLRWAVLGSLAPYALVAPVVWRMQEPPRGEPAHTRASYLVTLRRALAA
                     VRDDAGLRWMLLLQIALYGAQPAFFLLAQPFMRQHEVPLVLFGVLVAPVHLVWAGTGL
                     ISGRLVRRIGLAGTLGAAIGLGTGGLLVLTAIDHVMAFSGLAACMAGAGIAFPAIGSY
                     VNDRTASEVRATVLSIAPLGSSLVLGMAGIGVGALAQRSLQLAFGAMALVVLIAAGGC
                     FLAWMGAESRTARAVGPAVTDAAS"
     gene            2159..>2876
                     /locus_tag="PLX85_05440"
     CDS             2159..>2876
                     /locus_tag="PLX85_05440"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRC62649.1"
                     /translation="MLELPPPEVERRPVTLAARLLQGGPVALVTTADRGNANVLPVAW
                     MMPLSTRPPLVAIAIEQSRHSAEMISHSEQFALNFPGRTLLHHVQYLASLSGKDIDKF
                     EATQLETFVAAHLSAPLLRDCLAWVECEVQKVLPFGDHFLYVGLVVAVHVDPQAFDEQ
                     WLLESDDARPLHFLGGNRYSTLDGLLEARLPRASEAPERVLSERVQEELELTREARER
                     REELIDEVLRDVDGGKVVDLG"
BASE COUNT          466 a         1103 c          907 g          400 t
ORIGIN      
        1 cgccgccatg ggagccccga ggtcccagcc cgcattcgcc cgcgtctcga cctgtgcgcc
       61 cgcaacgccg aggaacgcgc tggggtggat cgcgtcagcc gggttggcgg taatcggggg
      121 cagcaccatc tcctcgggcg gccatggccc gtcgatgacc tcccgcagcc ggctgatctg
      181 caggtccggg atcttcggca ggatcagatg ccacgggtcg acctcgtaga tgtcgatggt
      241 ggcgccgcgc accgggagcg tcagggtcac gccgcccgat ccgacgcgct tcaccaactt
      301 gccgcgcacc aggcagctcc gcagcatgag cctcgcgacg tccggcgccc cgaggcgcac
      361 gtgcagcggg ttcagccgct cgatgttcgg ccgcagcgcg acccgctcct cgaaggctcc
      421 gcgacgcagc agctcaccgg cgtccggctc ttcgccctcg agctccggcc cgatgagcag
      481 gcgcatcgtg tccggttcac tccgcgtcgg aatcggcagg cgcgtcgcgc ccgaggcgtt
      541 cagcgcctcc gatgcaatca gcctgccccc gctcgtgaat gcgtacgccc gcagcgcggg
      601 caggccgtca ggccgctcag cgaggtccgc ctgcacctcg agtgcgagcg accgacctct
      661 cggcgttctc gtgaccatcg gtaggttcct cctgttcgtg cgatccccgg tggacggtcg
      721 cactgtaggg aggatgtaaa ttcatatctg tgagctgctt cacactgccg cgtgtatgtt
      781 tccggcacgg cggagccaag aactgcgtgc tggcattcgc ctaggaagcc gcgtccgtca
      841 ccgcgggccc cacggccctc gccgtccgtg actccgcgcc catccacgcg aggaagcagc
      901 cgccggccgc gatcagcacg accagcgcca tcgccccgaa cgccagctgg agcgaccgtt
      961 gcgccagcgc gccgaccccg atgcccgcca ttccgagcac cagcgaactg ccgagcggcg
     1021 cgatcgagag caccgtcgcc cgcacctccg aggccgtgcg gtcgttcaca taggagccga
     1081 tcgccgggaa cgcaataccg gcgcccgcca tgcacgcagc gaggccggag aacgccatca
     1141 cgtggtcgat ggccgtcagc accagcaggc cgcccgtccc cagcccgatc gcggcgccca
     1201 gggtcccggc aagcccgatg cggcgcacca ggcgcccgct gatcaggccc gtccccgccc
     1261 acacgaggtg caccggcgcg accagcacgc cgaagaggac cagcggcacc tcgtgttgcc
     1321 gcatgaacgg ctgcgcgagc aggaagaacg ccggctgtgc tccgtacagc gcgatctgca
     1381 ggagcagcat ccagcgcagc cccgcgtcat cgcgcacggc ggccagcgcc ctgcgcaggg
     1441 tgacgaggta actcgcccgc gtatgcgccg gctcgccgcg cggcggctcc tgcatccgcc
     1501 agaccaccgg ggccaccagc gcgtacgggg ccagcgaacc cagcaccgcc caccgcagcc
     1561 cggccaggtc cgccagcagc ccgccgagca acccgccgat cagcaccgcg ctgatgctga
     1621 gcgcccagta cacaccgatg cggtccccga actccgcctg ccgctgaccc acggccagcg
     1681 cgtcgtacag gtatgcctcc tcgttccccg atcgaaacgc gagtcctgcc gcccagatgc
     1741 cgtagctcac catgagcagc ccgaacccgg ccgcgagagc gaataccacc accccgctcg
     1801 cttccagcac caggccggtc acgaacgtca gccgtcgccc atagcggtcg gcgaaggcgc
     1861 ccgagggaat ctccgccccg agcttgatca gccagaacag cccctccatc agacccacct
     1921 gcgccagcgt gaggtggcgg tagtccgtca ggtagacgac ccagatcacg agcagggggc
     1981 tcagctcgcg caggaagccg agcacgtaga agaggcggac gtttcgagtt acctcgaacg
     2041 atggcgagcg gtgaggcgca gcctgtgaca agcggagtac tcctcgtccg catccatact
     2101 aggtcacggc gccgcccgag cgcccggacg cagccggtcc gccccgtata ctcggatcgt
     2161 gctcgaactc cccccgcccg aggttgaacg ccgtccggta acgctggctg cgcgcctgct
     2221 ccagggcggt cccgtcgccc tcgtcaccac cgccgaccgc ggcaacgcga acgtgctgcc
     2281 cgtcgcctgg atgatgccgc tctccacgcg gccacccctc gtggcgatcg ccatcgagca
     2341 gtcgcgccac tccgccgaga tgatcagcca ctccgagcag ttcgcgctca actttcccgg
     2401 ccgcacgctg ctccaccatg tgcagtacct cgcctcgctc tccggcaagg acatcgacaa
     2461 gttcgaggcc acccagctgg agaccttcgt cgccgcgcac ctcagcgccc cgctgctgcg
     2521 tgactgtctt gcctgggtgg agtgcgaggt gcagaaggtg ctgcccttcg gcgaccactt
     2581 cctctacgtc ggcctcgtgg tcgccgtcca cgtcgacccg caggcattcg acgagcagtg
     2641 gctgctcgag tcggacgacg cgcgcccgct gcatttcctc ggcggcaacc gctactccac
     2701 gctcgacggt ctcctcgaag ctcgcctccc gcgggcgtcg gaggcgcccg agcgggtcct
     2761 ctccgagcgt gtgcaggaag agctcgaact tacccgcgaa gcgcgcgagc gccgcgaaga
     2821 attgatcgat gaagtcctcc gcgatgtcga cgggggtaaa gtcgtcgatc tgggcc
//
LOCUS       DAORPG010000204         2876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3570_length_2876_cov_3.046792, whole genome shotgun sequence.
ACCESSION   DAORPG010000204 DAORPG010000000
VERSION     DAORPG010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3570_length_2876_cov_3.046792"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /gene="glgP"
                     /locus_tag="PLX85_05445"
     CDS             complement(<1..1038)
                     /gene="glgP"
                     /locus_tag="PLX85_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRC62650.1"
                     /translation="MSVVAYFSMDLALESDIPTYSGGLGVLAGDALRSAADLGLPLVG
                     VSLLYRKGYFHQHLDAAGNQTETDDGWQPAGRLTELPARVTVHIEGREVCLRAWRYCI
                     RGVRGHEIPVFLLDSDLQDNEPTDRGLTDHLYGGDHRYRLAQEVVLGIGGVRLLRALG
                     YVDTTTFHMNEGHSALLALALLQERLGGRPTAHADAADLEAVRQQCVFTTHTPVSAGH
                     DKFQWDLVHHVLGEEHASFLQHTGSPGDRLNMTHLALRCSRYVNAVAMRHAEVSREMF
                     PGQTIHAITNGVHAGTWVAPSFQRLYDRHLPDWRLDSSNFRHAVGIPLHEIDLAHVEA
                     KRRLLHEVANRT"
     gene            complement(1200..2318)
                     /locus_tag="PLX85_05450"
     CDS             complement(1200..2318)
                     /locus_tag="PLX85_05450"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017557137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HRC62651.1"
                     /translation="MNSPHAGVILVVNAGSSSLKLRVLDAHDQVVEQADLPAIAGASD
                     LEAVRGLLARHPGIRAIGHRMVHGGPEFQTSTLITGDLLERLRAVSDLAPLHNPSALA
                     GLDAMIEAAPGLPNVVCFDTAFHASLPAAAATYAIPRQWTEAWGIRRYGFHGLNHSYL
                     ARRVPELLERPSEGLRLVSCHLGAGASLAAILDGHSIDTTMGFTPLEGLVMATRSGSL
                     DPGILIHVQRHHGLSVDELERVLDEESGLRGLSGSSGDMRAVERAVDEGDPLAALAFE
                     VYVHRLAGAIAAMAASLGGVDAIAFTGGVGEGSARLRHAATARLAFLGVTIDEATNER
                     AGDEDGVLSPEGAAVPVLLVHAREDLEIAREVRRTLAP"
     gene            complement(2315..>2876)
                     /locus_tag="PLX85_05455"
     CDS             complement(2315..>2876)
                     /locus_tag="PLX85_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HRC62652.1"
                     /translation="SNDTDGSPDVVMACCGDVPTLETLAAVDLLRQHLPELKVRVVNV
                     VDLMRLQSESEHPHGLSDLEYDTLFPPDVPTVFAFHGYPWLIHRLTYRRHGEDNLHVR
                     GYKEEGTVTTPFDMVMLNDLDRFRLVIDVIDRVPGLGSRAAHLRQRMVDERQRARAYT
                     RQYGEDMPAIADWTWPHGLTSEAESH"
BASE COUNT          459 a          965 c         1013 g          439 t
ORIGIN      
        1 cgtgcggttg gccacttcgt gcaggagccg ccgcttcgcc tccacatgcg cgagatcgat
       61 ctcgtgcagc gggatgccga ccgcgtggcg gaagttgctg ctgtcgagcc gccagtccgg
      121 caggtggcgg tcgtacagac gctggaagga cggagcgacc catgtccccg cgtggacgcc
      181 gttcgtgatc gcgtgaatcg tctggccggg gaacatctcg cgcgagacct cggcatgacg
      241 catcgccacc gcgttcacgt agcgcgagca gcggagcgcc aggtgagtca tgttcagccg
      301 gtcgcctgga ctccccgtgt gctgcaggaa cgaagcgtgc tcctcgccca ggacgtgatg
      361 aacgaggtcc cactggaact tgtcgtgccc cgcggaaacc ggcgtgtgtg tggtgaagac
      421 gcactgctgt cgcaccgcct cgaggtcggc cgcgtcggcg tgcgcagtcg gccgtccgcc
      481 gagccgttcc tggagcaagg ccagcgcgag caatgcggag tgaccctcgt tcatgtggaa
      541 cgtcgtcgtg tcgacgtacc cgagcgcgcg cagtagccgc acgcccccga tgccgagcac
      601 gacctcctgc gcgagacggt agcgatggtc gccgccgtag aggtgatccg tgagtcctcg
      661 atcggtgggt tcgttgtcct gcaggtcgct atcgagcagg aagacgggga tctcatggcc
      721 gcgcacgcct cggatgcagt aacgccacgc gcgcaggcag acctcgcggc cctcgatatg
      781 cacggtgacg cgcgccggca gctcggtcag tcgaccggcg ggctgccacc cgtcgtccgt
      841 ctcggtctgg ttgcccgctg catcgaggtg ctggtggaag tatcccttgc ggtacagcag
      901 ggacacgccg acaagcggca ggccgaggtc tgcggcagaa cgcagcgcgt ctcccgcaag
      961 cacgccgagc ccgccgctgt acgtcgggat atccgactcc agcgccaggt ccatcgagaa
     1021 gtaggctacg actgacatca gctccgccag tgggcgcagt cacgggccga tcgaccgcgc
     1081 acagccaccc tggggagcga ccgcgaaggc aacgacgagc acgccccgta ctcggaaacg
     1141 acccgacgat ggtacccgcc cggatcgagg gcttccagcc tacgcccctg gcctcgctgt
     1201 catggggcga gcgtccgtcg cacctctctc gcgatttcga ggtcctcgcg tgcatggacg
     1261 agcagcacgg gcaccgcggc gccctcgggc gaaagcacgc cgtcctcgtc gcccgcgcgc
     1321 tcgttcgtgg cttcgtcgat cgtgacgccg agaaacgcga ggcgtgcggt ggccgcgtgc
     1381 cgcaggcgcg ccgatccctc gcccacgccg cctgtgaagg cgatggcgtc gactcccccg
     1441 agggaggcgg ccatcgctgc gatcgcaccc gcgaggcggt gcacgtacac ctcgaacgcc
     1501 agcgcagcga gagggtcgcc ttcgtccacg gcccgctcga ccgcccgcat gtcgccgctg
     1561 ctgcctgaga gcccgcgcag gccggattcc tcgtcgagca cgcgttcgag ctcgtcgacc
     1621 gacaggccgt ggtggcgttg cacgtggatg aggatgccgg ggtccagcga gcccgaccgc
     1681 gtcgccatga cgaggccttc cagcggcgtg aagcccatcg tcgtgtcgat ggaatggccg
     1741 tcgaggatcg cggcgagcga cgcgcccgcg ccgaggtgac agctgacgag tcgcagcccc
     1801 tcggaaggcc gttcgagcag ctcagggacg cggcgggcga ggtaggagtg gttcaggccg
     1861 tggaagccgt agcggcggat gccccacgcc tcggtccact gccgcggaat ggcgtacgtc
     1921 gccgccgcgg ctggcagcga ggcgtggaac gccgtgtcga agcacacgac gttggggagg
     1981 ccgggcgccg cctcgatcat cgcgtcgagc cccgcgagcg ccgacggatt gtgcaacggc
     2041 gcgaggtcgg acacggcacg caagcgctcg agcaggtcgc cggtgatcaa cgtgctggtc
     2101 tggaactcag ggccgccgtg caccatgcgg tggccgatgg cacggatgcc gggatgccgc
     2161 gccagcagcc cgcgaaccgc ctcgaggtcg ctcgcgccgg cgattgcggg cagatccgcc
     2221 tgctccacga cctgatcgtg tgcatcgagg actcgcagct tcaggctgct cgaccccgcg
     2281 ttcacaacca ggatcacgcc ggcgtgcgga ctgttcaatg ggattcggcc tctgaggtga
     2341 gcccatgggg ccaggtccag tcggcgattg cgggcatgtc ctcgccatat tgcctcgtat
     2401 aggcgcgagc gcgctgccgc tcgtccacca tgcgctgtcg caggtgggcg gcacgtgagc
     2461 cgaggccggg cacccggtcg atgacgtcga tgacgaggcg gaagcggtcg aggtcgttca
     2521 gcatcaccat gtcgaagggc gtggtgaccg tgccttcctc cttgtagccg cgcacgtgga
     2581 ggttgtcctc tccatgccgt cggtacgtca gacgatgaat cagccagggg tagccgtgga
     2641 aggcaaacac cgtcgggacg tccggcggga acagcgtgtc gtactcgagg tcggagaggc
     2701 cgtgcggatg ctccgactcg gactgcaggc gcatgagatc gacgacgttg acgacgcgta
     2761 ccttcagctc cggcaggtgc tgacgcagca aatccaccgc cgccagcgtc tcaagggtcg
     2821 gcacgtcgcc gcaacaggcc atgacgacgt cgggcgagcc gtccgtgtca ttgctt
//
LOCUS       DAORPG010000205         2875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3573_length_2875_cov_4.292908, whole genome shotgun sequence.
ACCESSION   DAORPG010000205 DAORPG010000000
VERSION     DAORPG010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3573_length_2875_cov_4.292908"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PLX85_05460"
     CDS             complement(<1..249)
                     /locus_tag="PLX85_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRC62653.1"
                     /translation="MARRNRPPTRPVAPDPRFQSRLVTSFTNKLMHNGKKSVAERIVY
                     SALDRVEQQTGRSSIDVFDQAVRNATPVIEVKPRRVGGA"
     gene            complement(260..673)
                     /gene="rpsL"
                     /locus_tag="PLX85_05465"
     CDS             complement(260..673)
                     /gene="rpsL"
                     /locus_tag="PLX85_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRC62654.1"
                     /translation="MPTINQLVRHSRQPVIKKTKAPALRFRFNALKSRTTRSPKGSPQ
                     KRGVCTQVRTVTPKKPNSALRKVARVRLSNGIEVTAYIPGEGHTLQEHSVVLIRGGRV
                     KDLPGVRYHIIRGALDAQGVKDRKRGRSKYGTRKG"
     gene            1126..1521
                     /gene="mscL"
                     /locus_tag="PLX85_05470"
     CDS             1126..1521
                     /gene="mscL"
                     /locus_tag="PLX85_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRC62655.1"
                     /translation="MVSGFRDFIMRGNVIDLAVAVVIGAAFGAVVTALVENLINPLIA
                     AIGGQPDLSSVATFTINNAQFSIGAVLNAAINFLIIAAAVYFVLVMPVNRLMEMRKRK
                     EVAEVAATPEDIALLQEIRDLLKARGGAV"
     gene            1605..2237
                     /locus_tag="PLX85_05475"
     CDS             1605..2237
                     /locus_tag="PLX85_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62656.1"
                     /translation="MTELFYTTALSRRASGLGWATRTARFEYAPVQANIQLSYYDAQN
                     GERFEPFQPRFARTWIETPMTPTTVVAGMTIPMPNVLKDSTVWLTTDRLTFQLQTENV
                     TAVAVGMIWDRRPDLGRWGRASRLTAGVTLDLAIHDEAGNVIGTHQEVQLEGGRPIDP
                     EEVQARVLDRAQATSNARLSAAIVDLSQLPEDASFRIDTSSGRAFPLETE"
     gene            complement(2309..>2875)
                     /locus_tag="PLX85_05480"
     CDS             complement(2309..>2875)
                     /locus_tag="PLX85_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62657.1"
                     /translation="VASFFLGGSDDEASPAPTATATPTIEVRSSPTLTPTRLATSTPT
                     PVPNVTPTSTPTPAPTQLPVTPAPLPTEPPRPAPTQAPPITSTPANFAGVWRIADTVL
                     QGRGAGQTFTFEVSITQTGNAIQGGAAGVIALAGTAAGNIATVEFSQPALGITGIFIW
                     TLGADGNATGTFTSNVPNSGTSQLIRLR"
BASE COUNT          476 a          821 c         1102 g          476 t
ORIGIN      
        1 ggcgccaccg acgcggcgag gcttgacctc gatgacgggg gtggcgttcc ggacggcctg
       61 gtcgaagacg tcgatggagg agcggccggt ctgctgctcg acgcggtcga gcgcgctgta
      121 gacgatgcgc tcggcaacgc tcttcttgcc gttgtgcatg agcttgttgg tgaacgaggt
      181 caccaaccgc gactggaagc gcgggtcagg cgcgaccggg cgggtaggcg ggcgattgcg
      241 acgtgccatc tcgctgctcc tagcccttcc gcgtgccgta cttgctgcgg ccgcgcttgc
      301 gatccttgac gccctgggcg tccagagcgc cgcggatgat gtggtagcgg acgccgggca
      361 ggtccttcac acgaccgccg cggatgagga cgaccgagtg ctcctggagg gtgtgcccct
      421 cacccgggat gtacgcggtg acctcgatgc cgttggagag gcggacacgc gcgaccttgc
      481 gaagcgcgga gttcggcttc ttgggggtga cggtgcgcac ctgagtgcag acgccgcgct
      541 tctggggcga gcccttgggc gagcgggtcg tgcggctctt gagcgcgttg aagcggaagc
      601 gcaacgcggg cgccttggtc ttcttgatga ccggctggcg ggaatgccgg accagctgat
      661 taatcgtcgg cactgcgact ccactcgcaa acgcccgggc tacggcaggt tgtccaccga
      721 ggtggaggcc atatcgaggg cagcaagaac ggccggaagg ctgcgcggtg aaccattgcg
      781 gcgggagcgc cctcaagggg cgcgggtcag ggtgccgctg gtcgtcaccg cgttcgtgcc
      841 accgaccgcc acacgtgaac gtgcgtcgtt ctcggaacgc gaacttggat gttacgggcg
      901 aggtggaact gtgtcaacgc tctatcggag cggaggggga gcggcgcaac tgccgcctcg
      961 atgaagagaa tactagcgaa cgggtgtacg cgcaggcaag tgatcaattg cggttgaccg
     1021 gggtgaacag gcgtttactc gccgcgcatc gccgtcacgt atcatgcgcg gcggcgagtg
     1081 ggggcatcgg cttcgatgca gggaaaggag ttgaagatga acaacatggt tagcgggttc
     1141 cgagacttca tcatgcgggg gaacgtgatc gacctcgctg tcgcggtggt catcggcgcg
     1201 gcgttcgggg cggtcgtgac agcactcgtg gagaacctga tcaacccgct gatcgcggcg
     1261 atcgggggcc agccagacct gagcagcgtg gcgacgttca cgatcaacaa cgcgcagttc
     1321 tcgatcgggg cggtgctgaa cgcagcgatc aacttcctga tcatcgcggc ggcggtgtat
     1381 ttcgtgctcg tgatgccggt gaaccggctg atggagatgc gcaagcggaa ggaagtcgcc
     1441 gaggtggcgg ccacgcctga ggacatcgcg ctgctgcagg agatccgtga cctgctgaag
     1501 gctcggggcg gggcagtcta ggacacttcg atcgggtgta tccagaaggc ggcgggggag
     1561 acccgccgcc ctcgatttca ggtgtgcaag cgggaggtgg tcggatgact gagctgttct
     1621 acacgacggc cctgtcccgt cgtgcgtcgg ggcttgggtg ggccacgcgt acggcgcgct
     1681 tcgagtacgc gccggtgcag gcgaacatcc agttgagcta ctacgacgcg cagaatggtg
     1741 agcgcttcga gccattccag cctcgattcg cgcgtacgtg gattgagacg ccgatgacgc
     1801 ccacgacagt ggttgccggg atgacgatcc caatgccgaa cgtgctcaag gacagcacgg
     1861 tgtggctgac aacggaccgt ctcacgtttc agttacagac ggagaacgtg acggctgtgg
     1921 ctgtcgggat gatctgggac cggcgacccg acctggggcg gtgggggcga gcgagccggc
     1981 tgaccgccgg cgtgacgctc gacctcgcga ttcatgacga ggccgggaat gtgatcggga
     2041 cgcaccagga ggtgcagctg gaaggcgggc gcccgatcga ccccgaggag gtgcaggcgc
     2101 gcgtcctcga ccgagcgcag gctacctcaa acgcccggct gagcgccgcg attgtcgacc
     2161 tcagccagct cccggaggat gcaagcttcc gcatcgatac gagctcgggt cgggcgtttc
     2221 ccctcgaaac ggagtagcgc gcgcgggcgg cccagcaaag aggcgacagg gaaactcgcc
     2281 gccgcttcgt ctgcccccga acccggggtc agcgcagccg gatgagctga ctcgtgccgc
     2341 tattcgggac gttgctggtg aacgtgcctg tagcgttgcc gtcggcgccg agcgtccaga
     2401 tgaagatgcc ggtgatgccg agggcgggct gcgagaactc gacggtggcg atgttgccgg
     2461 cggcggtgcc agcgagggca atgacgccgg ccgcgccgcc ttgaatcgcg ttgcccgtct
     2521 gggtgatcga gacctcgaag gtgaaggtct gtccggcacc tcgtccctgg aggacagtgt
     2581 ccgcgatgcg ccagacgccg gcgaagttcg cgggcgtgga ggtgatgggc ggcgcctgcg
     2641 tcggtgcggg acgaggcggc tcggtcggga gaggagccgg agtcaccgga agctgcgtcg
     2701 gtgcgggggt gggggtggag gttggcgtga cgttggggac cggcgtcggc gttgaagtcg
     2761 caaggcgcgt cggggtcagc gtcgggctgg atctgacctc gatggtgggc gtggcggtgg
     2821 ccgtcggggc agggctggcc tcgtcatcgg agccgccgag gaagaaggag gcaac
//
LOCUS       DAORPG010000206         2870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3592_length_2870_cov_3.801421, whole genome shotgun sequence.
ACCESSION   DAORPG010000206 DAORPG010000000
VERSION     DAORPG010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3592_length_2870_cov_3.801421"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(455..2566)
                     /gene="uvrB"
                     /locus_tag="PLX85_05485"
     CDS             complement(455..2566)
                     /gene="uvrB"
                     /locus_tag="PLX85_05485"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRC62658.1"
                     /translation="MPVATDQLFELVTPLVPTGDQPEAIDRLVAGLERGERLQTLLGA
                     TGSGKTFTIANVVARYQRPTLVIAPNRTLAAQLTSEFRDFFQKNAIEYFVSYYDYYQP
                     EAYIPRSDTYIAKDSDVNDEIDKMRHAATRSLFERRDVLIVASVSCIYGLGEPGEYES
                     FVVDLYRGRHINRASLVRQLVDMQYQRNDINLARGSFRVRGDSLTIIPAYEELAVRVD
                     FFGDEVERIVTVDPLSGELLSEVERHSIYPAKHFVTSKERMEHALVDIDEELGQRLAE
                     LRGQGKILEAARLEERTRFDLEMLREAGYCSGIENYSRHLAGRAQGSTPWTLLDYFPD
                     DWLLFIDESHISIPQIRGMYHGDVARKQTLVEYGFRLPSALDNRPLNFDEFERHINQA
                     VFVSATPGPYEMDVSSQVVEQVIRPTGIVDPMVDVRPTKGQIDDLLAEVRERVARQER
                     VLVTTLTKKMAEDLTDFLLEMGVKASYLHSEIDTLQRIEILRDLRHGVHDVVVGINLL
                     REGLDLPEVSLVCILDADKEGYLRSGGSLIQTIGRAARHAEGKVIMYADTITDSMRTA
                     LDETDRRRAKQLRYNEEHGITPQGISKTIRDITDQLRVAEEQAAYTTASDLPKDEIVR
                     LVRELESQMKAAARNLEFERAALLRDQVVELRRELVGDTPDALGRFIGDSVPASSSPS
                     AVPTRSGGRMRNGRGTRRPRR"
     gene            complement(2631..>2870)
                     /locus_tag="PLX85_05490"
     CDS             complement(2631..>2870)
                     /locus_tag="PLX85_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RuvA C-terminal domain-containing protein"
                     /protein_id="HRC62659.1"
                     /translation="IIAHLRGKLVQEALLQDRDGHTPVAAPDIRADAVDALVALQYGR
                     REAEALVQEAFRTREDLDSLEAVLRHVLEQQARGG"
BASE COUNT          469 a          916 c          958 g          527 t
ORIGIN      
        1 ccctgcgctc ctacggggat gacgtctgag attcggtgcc agcgggcaca tccgcgagcc
       61 ctcgtccgaa cgcttgcgaa acctcgcgat tgagcccacg ccgggtgagg caccgcgcgc
      121 gagatgccga ccatcgaact tgccgcgtcc tcgcatactg gattcaacaa ggaaggttgc
      181 cgcggaccgg agggctcgac ccttggacaa cgtggatcga tcttatcgct cggaccgtca
      241 cggctaacca gccgttgacg ctcaaagagc ccgtgaggcc ccgccccagc ggggcctcat
      301 gttcgacagc ggctccgttg cagggcagtc gaacgcgcgc ggctggacgg caactcccgt
      361 gctcctcggc gctcaggggt ggcgtcgtcc cgaggcggag ccgtcgtcgc ggctcctcac
      421 gagagtcgcg aacgttcggg ccaggtcgat ttcgttagcg tcgcggtcgg cgggtgcctc
      481 gtccgttgcg cattcgcccg ccgctgcggg tcggcaccgc cgacgggctt gacgaggccg
      541 ggactgagtc accgatgaag cgaccgagcg cgtcgggcgt gtcacccacc agttcacgac
      601 gcagttccac cacctgatcg cggagcagcg ccgcgcgctc aaattcgagg ttgcgggccg
      661 cggctttcat ctgcgactcg agctcacgga ccaggcggac gatttcgtcc ttcggcagat
      721 ccgatgccgt cgtgtaggcc gcctgctcct cggcgacccg cagctgatcg gtgatgtcac
      781 gaatcgtctt cgagatcccc tggggggtga tgccgtgctc ttcgttgtac cggagctgct
      841 tcgcgcggcg gcggtcggtc tcgtcgagcg ccgtgcgcat cgagtccgtg atcgtgtcgg
      901 catacatgat caccttgccc tcggcgtgac gagccgcccg gccgatcgtc tggatcagcg
      961 agccccccga ccgcagatag ccttccttgt ccgcgtccag gatgcagacg agggatacct
     1021 cggggaggtc gaggccttcc cgtagcaggt tgatgccgac gactacgtca tgtacgccgt
     1081 gacgcaggtc tcgcagaatc tcgatacgct gcaacgtgtc gatttccgag tgcaggtagc
     1141 tggccttcac ccccatttcg agaaggaagt ccgtcaggtc ctcggccatc ttcttcgtga
     1201 gcgtggtcac cagcacgcgc tcctggcggg ccacacgctc gcgcacctcg gcgagcagat
     1261 cgtcgatctg ccccttggtc ggacgtacgt cgaccatggg atcgacgata cccgttgggc
     1321 ggatcacctg ctcaacgacc tgcgaggaca catccatttc gtaggggccg ggcgtcgccg
     1381 aaacgaacac ggcctgattg atgtgccgct caaactcgtc gaagttcagc gggcggttgt
     1441 cgagggctga cggcaggcgg aagccgtact cgaccagcgt ctgctttcgc gccacatcgc
     1501 cgtggtacat gcctcggatc tgcgggatgg agatgtgcga ctcgtcgatg aagagcagcc
     1561 agtcgtccgg gaagtagtcg agcagagtcc acggagtcga gccctgtgct cgacccgcga
     1621 ggtgccgcga gtagttctcg atacccgagc agtaaccggc ctcccgcagc atctcgaggt
     1681 cgaagcgcgt gcgttcctcg aggcgcgcgg cctcaagaat cttgccctgt ccacgcagct
     1741 ccgccaggcg ttgtcccagc tcctcgtcga tatcgacgag ggcgtgctcc atgcgctcct
     1801 tcgaggtcac gaagtgcttc gcggggtaga tggagtgacg ctcgacctcg ctgagcagtt
     1861 cgcccgatag cgggtcgacc gtgacgatgc gctcgacctc gtcgccgaag aagtcgacgc
     1921 gcacggccag ctcctcgtag gccgggatga tcgtgagtga atcgccgcga acccggaatg
     1981 acccccgggc gaggttgatg tcgttgcgct ggtactgcat gtcgacgagc tgacgcacga
     2041 gcgaggcccg gttgatgtgg cgaccgcggt aaaggtcgac gacgaacgac tcgtactcgc
     2101 cgggctcgcc caggccgtag atgcaggaca cagaggcaac gatgagcaca tcgcgccgct
     2161 cgaacagaga gcgcgtagcg gcgtgccgca tcttgtcgat ctcgtcgttt acgtccgagt
     2221 ccttggcgat gtaggtgtcg ctgcgcggga tgtacgcctc gggctggtag tagtcgtagt
     2281 aggagacgaa atattcgatc gcgttcttct ggaagaagtc gcggaactcc gaggtgagct
     2341 gggcggccag cgtgcggttt ggagcgatga ccaaggtcgg gcgctgatag cgtgcgacga
     2401 cgttcgcgat cgtgaacgtc ttgccagacc cggtcgcccc caacagcgtc tgtagccgct
     2461 cgccgcgctc gagaccggcg acgaggcggt cgattgcctc gggctggtcg ccggtgggca
     2521 cgaggggtgt gacgagctcg aagagctgat cggtggcaac gggcacgagc ggatcctttg
     2581 ggctaccgat ccattctagc gatcgactgc cggctcgttg tccgacctgc ctatcctcct
     2641 cttgcctgct gctcgaggac gtgccggagc accgcttcga gactgtcgag gtcctcgcgc
     2701 gtgcggaagg cctcttgcac gagggcctcc gcctcgcgtc ggccgtattg cagcgcgacc
     2761 aacgcgtcga cggcatccgc acgtatgtcg ggcgcggcga cgggtgtgtg gccatcgcga
     2821 tcctgcagca gcgcttcctg cacgagcttg ccgcgcaggt gcgcgatgat
//
LOCUS       DAORPG010000207         2866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3604_length_2866_cov_3.425116, whole genome shotgun sequence.
ACCESSION   DAORPG010000207 DAORPG010000000
VERSION     DAORPG010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3604_length_2866_cov_3.425116"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PLX85_05495"
     CDS             complement(<1..847)
                     /locus_tag="PLX85_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HRC62660.1"
                     /translation="MLFSGYDTGPWFDELFQPGGTPRPEAAELVHAIEALPPGGFERL
                     QETANAALVNLGITFNVYSDGAGIERVLPFDMVPRIVTASEWERIERGLQQRIYALNC
                     FIDDVYHDGKILSDGVVPRELVLSAPAYREACVGLNPPRGIWCHITGTDLVRDGDGQV
                     YVLEDNLRCPSGVSYVLENRRLMKRTFPGLFSSSHVRPVDDYASRLLETLSALASREE
                     APEVAVLTPGVYNSAYFEHSYLAQQMGVELVEGRDLVVDDDTLYMRTTRGLERVDVLY
                     RRIDDD"
     gene            complement(1003..2217)
                     /locus_tag="PLX85_05500"
     CDS             complement(1003..2217)
                     /locus_tag="PLX85_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRC62661.1"
                     /translation="MSERIYLDHAATTPPDPRVVEAMLPYLRDAWHNPSSIYVEAQAA
                     RAAIDEARETVARSIGARPEEIVFTSGGSESDSLALRGVLAASAHRGRHLITSAVEHH
                     AVLDTAEQLEREGAEVSIVGVGPDGRVDPTEVEAAIRDDTVLVSIMHANNEVGAINDL
                     AEIARRVHARNPHTMVHTDAVQSAAHLGVHVDRLGVDLLTLTAHKFYGPRGAGVLYAR
                     SGTPLQPQILGGGQEDNRRAGTENTAAIVGLAEAVRVAEEQREADISHERHLQQRLIE
                     ELPRRVPMLGISGPRDLSLRLPGSVSVIVAFVEGESILLALDLAGVAASSGSACTTGS
                     VEPSHVLVAMGVPPDLARGSLRFTLGRMNTDADVDRLLEVLPPIVQRMRALSPLPVTE
                     PPRAYRTWLANA"
     gene            2321..>2866
                     /locus_tag="PLX85_05505"
     CDS             2321..>2866
                     /locus_tag="PLX85_05505"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HRC62662.1"
                     /translation="MATVGRLESSREAILDILRRRDGASVDELSHELGLAGATVRRHL
                     DVLMRDGYVSASQVRGGMGRPRYSFSITEAGAELFPHHYVRLTRRLIDEIVALGPSET
                     TGRPGIEIANLIFEKMAERLAREYGPRVEGRTLEERVRSTARLLAEEGIDFEVAATAR
                     GVQLLGRGCPCQRLGGLATTFE"
BASE COUNT          460 a          895 c         1061 g          450 t
ORIGIN      
        1 agtcgtcgtc gatgcggcgg tagaggacgt cgacgcgttc gaggccacgc gtggtccgca
       61 tgtagagcgt gtcgtcgtcg acgacgaggt cgcgtccctc gacgagctcg acccccatct
      121 gctgggcgag ataggagtgc tcgaagtagg cggagttgta gacgccgggc gtgaggacgg
      181 ccacctcggg cgcctcttcg cgggatgcaa gcgcggacag cgtttcgagg agccggctcg
      241 cgtagtcgtc cacgggccgt acatgggagg agctgaacag gcccgggaac gtgcgcttca
      301 tcaggcggcg gttctcgagc acatatgaca cgcccgaggg gcaacgcagg ttgtcctcga
      361 ggacgtagac ctggccgtcc ccgtcgcgca cgaggtcggt gcccgtgatg tggcaccaga
      421 ttcctcgcgg cggattgagg ccgacacacg cctcgcggta tgcgggcgcg gagaggacga
      481 gctcgcgagg cacgacgccg tcgctcagga tcttgccgtc gtggtagacg tcgtcgatga
      541 agcagttcag ggcgtagatg cgttgctgga gccctcgttc gatgcgttcc cactcggatg
      601 cggtcacgat gcgcgggacc atatcgaacg gcagcacgcg ttcgatcccg gcgccatcgc
      661 tgtagacgtt gaacgtgatg ccgaggttca ccagggccgc gtttgctgtc tcctgcagac
      721 gctcgaaccc gcccggaggg agcgcctcga tggcgtgcac cagctcggcg gcttccggcc
      781 gcggggtgcc acccggctgg aacagctcgt cgaaccacgg tccagtgtcg tatccggaga
      841 agagcacctg tcgcctcggt caacggcgcg agaacgccgc gcgtactgca ctgaccaagg
      901 tagcggacac acgggcccgc gagtgacatg gccgttacga aatcgttaca aacgcgagaa
      961 gtcgcgcgat cgaaccgaag tcgcccgcgt gaggacgagg tgctaggcgt ttgcgagcca
     1021 cgtgcggtac gcacgtggag gctcagtgac cggcagcggc gaaagcgcgc gcatgcgttg
     1081 cacgatcggc ggcaacacct cgagcagccg gtccacgtcc gcatcggtat tcatgcggcc
     1141 gagggtgaag cgaagcgacc cgcgggcgag gtcgggcggc acgcccatcg cgaccaggac
     1201 gtggctgggc tcgacggatc ctgtggtgca ggccgagccc gaggaggcgg ccacgccagc
     1261 caggtcgagc gcgagcagaa tcgactcgcc ctcgacgaag gcgacgatga ccgagacgga
     1321 gccgggaagc cgcaggctga gatccctcgg gccggagatg ccgagcatgg ggacgcgtcg
     1381 aggcagctcc tcgatcaggc gctgctgcag atggcgttcg tgactgatgt cggcctcgcg
     1441 ttgctcctcg gcgacacgga ccgcctcggc gaggccgacg atagcggccg tgttctcggt
     1501 gccggcgcgg cggttgtcct cctgcccgcc accgaggatc tgcggctgga gcggcgtacc
     1561 ggagcgggcg tacaggacgc cggcgcctcg cgggccatag aacttgtgcg ccgtcagcgt
     1621 gagcaggtcg acgccgaggc ggtcgacatg caccccgagg tgcgcggcgc tctgcaccgc
     1681 gtcggtgtgc accatggtgt gggggttacg ggcgtgcacc cggcgtgcga tttcggcgag
     1741 gtcgttgatg gccccgacct cgttgttcgc gtgcatgatc gagacgagga ccgtgtcatc
     1801 acggatggcg gcttcgacct cggtcgggtc aacgcggccg tcggggccca cgccgacgat
     1861 gctgacctcg gctccctcgc gttcgagctg ttcggccgtg tcgagaacgg cgtggtgctc
     1921 gacggccgag gtaatgagat gacggccgcg atgcgccgag gcggcaagca cgccgcgcag
     1981 ggcgaggctg tccgactccg agccgccgga agtgaagacg atctcctcag gccgggcgcc
     2041 gatcgacctc gcgacggtct cacgcgcctc gtcgatggcc gcacgtgccg cctgggcctc
     2101 gacgtagatt gaagaggggt tgtgccaggc gtcccgcaga taggggagca tcgcctccac
     2161 cacgcggggg tcgggcggcg tcgtcgcggc atggtcgagg tagatgcgct cgctcatccg
     2221 tccatggtac ggcctggccg gggcgaggtg tttacaacct ttctcgcaac aaaagcgtgg
     2281 aaagtttgac gcggctggcg gccgtgcgta cgctcgaatt atggcaacgg taggccgcct
     2341 cgagagcagc cgcgaagcga tcctcgatat tctccggcgc cgcgacggcg cttcggtgga
     2401 cgaactgtct cacgagctgg gcctggccgg ggcgacggtg cgccggcacc tcgatgtgtt
     2461 gatgcgtgac ggctatgtca gcgcgtcgca ggtgcgaggc gggatggggc ggccgcgcta
     2521 ctcgttctcg atcaccgagg cgggcgccga gctgtttccg caccactacg tgcggctgac
     2581 gcgccgtctg atcgatgaga tcgtggcgct ggggccgtcg gagacgacgg gccgtccggg
     2641 catcgagatt gcgaacctga tcttcgagaa gatggccgaa cggctggccc gggagtacgg
     2701 gccgcgtgtc gagggccgca cgctcgagga gcgcgtccga tccacggctc ggctgctggc
     2761 cgaagaaggc atcgacttcg aggtggccgc gacggcccga ggtgtccagc tcctcggacg
     2821 aggttgcccc tgccagcgcc tcggcgggct ggcgaccacg ttcgag
//
LOCUS       DAORPG010000208         2866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3605_length_2866_cov_3.170402, whole genome shotgun sequence.
ACCESSION   DAORPG010000208 DAORPG010000000
VERSION     DAORPG010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3605_length_2866_cov_3.170402"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PLX85_05510"
     CDS             complement(<1..402)
                     /locus_tag="PLX85_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HRC62663.1"
                     /translation="MRLTPLVMFVCAVACASGGTEATPSPAADPTAAAPSGLDPVILV
                     HGWMGSPRDLLSMKAFLDARGLRSFIAVLPGEDNVQNAASLRDYVNEVQALTRAERVD
                     IVAFSMGGLSARYYLRNLGGQDHVRRYISIDT"
     gene            644..1936
                     /locus_tag="PLX85_05515"
     CDS             644..1936
                     /locus_tag="PLX85_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62664.1"
                     /translation="MASRRLIWVGLYGVDAQPLDALPQFSHCFSLRVPLQHPRIPGVA
                     AEDRLGLRASEQGLSAVLALKVLHDEVASACGSPAAIVAFAPFPFFADLLGEYPHASH
                     LGSPWDEFEALNQKRYVEDQLRAFAPQNLNFIDWRPLPQGSSRVEVVRDELARHPIVL
                     RTASSHAGVGHELLTDEAMLASSRLLDDPSAVLGPFLAEHIPVTVSACVFPNGDITLH
                     CPAIQLIGLSHAKSAFTYCGNDTGAIRQLPRPLLDDLDACARTVGRWLHRRGYVGAFG
                     VDALVHEGRLLFGELNPRFQGSIRISNALDAALGRPDILQDHLMAWLGIPAIPSPPLA
                     ELVAAQPARSQVLLFNNSAARTRVRRHDAPLPEGAEMLLAPAEHVLVDPGELAFVLEF
                     ERSVTTDGRSLEANIAATVQASLATALETQDARRRGRR"
     gene            complement(2049..2402)
                     /locus_tag="PLX85_05520"
     CDS             complement(2049..2402)
                     /locus_tag="PLX85_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020523919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRC62665.1"
                     /translation="MASAEQKRFGSPDETRTFERGQVDLIEIGGGQVGRLTLQPGWRW
                     SEHVKPIAHTEYCEAPHFQYHVSGTLRILMEDGREFDAVPGDVTALPSGHDAWVVGDE
                     PVVVVDWFGASNYAQ"
     gene            complement(2580..>2866)
                     /locus_tag="PLX85_05525"
     CDS             complement(2580..>2866)
                     /locus_tag="PLX85_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019886158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC62666.1"
                     /translation="EARGLLEELGMGGELTRLELLERAEPRAGGPAGLSAREVEVLRL
                     IVEGKTNREIAAALVISERTVANHLAHIFTKIDADNRAAAAAFALRNGLA"
BASE COUNT          427 a         1072 c          867 g          500 t
ORIGIN      
        1 cgtatcgatc gagatgtacc gccgcacgtg gtcctggccg cccagattac gaaggtagta
       61 gcgtgccgat agcccgccca tcgaaaacgc cacgatgtcg acccgttcgg cccgcgtcag
      121 agcctgcact tcgttgacgt agtcacgtag cgaggccgcg ttttgcacat tgtcctctcc
      181 ggggaggacc gcgatgaacg accgcaatcc gcgagcatcg aggaacgctt tcatggacag
      241 gaggtctcgt gggctgccca tccagccgtg cacgaggatc acaggatcca gcccagacgg
      301 ggcggccgcc gtcgggtcgg ccgcgggtga gggcgtggct tccgtgcctc cactcgcaca
      361 cgcgaccgcg cagacgaaca tcaccagggg cgtcaggcgc atgcagcgaa gcatcccctc
      421 cacagtagac cgctcgcccg acgcgaggcc agctgaagga agcccgcttg ccggtcccac
      481 tcgacttcga gcgggctcgc gctcctgggt ccgtgcgcaa acgcgcccat atctgcgcag
      541 accgtgcact accggctacg atgtgctccc tccggggagg cgcagcgccg cgaggcaggt
      601 cctattgaat cgagaccggc tgtatagcct catcgaggct cgcttggcgt ctcggcgcct
      661 gatctgggtc ggcctgtatg gcgtcgacgc ccagcccctc gacgccctgc ctcagttctc
      721 gcattgcttc tccctgcgcg tcccgctgca acacccgcgc attccgggcg tggctgccga
      781 ggatcgtctc gggttgcggg cgtccgagca gggactgagt gccgtcctcg ccctcaaggt
      841 gctccatgat gaagtggctt ccgcctgtgg ttcacccgct gcaatcgtcg cgttcgcccc
      901 gtttccgttc ttcgctgact tgcttggcga gtacccgcat gccagccatc tcggatcgcc
      961 gtgggatgaa ttcgaagcgc tcaatcagaa acgctacgtc gaggatcaac tgcgcgcgtt
     1021 tgcgccgcag aatctgaact tcatcgactg gcgtccccta ccgcagggct catcgcgggt
     1081 cgaagtcgtc cgcgacgaac tcgctcgcca tccgatcgtg cttcgcaccg cctcgtccca
     1141 tgcgggcgtc ggacacgaat tgctgacgga tgaagcgatg ctcgcgagca gccgcctgct
     1201 cgatgacccg tccgcggtgc tcggtccatt cctcgccgaa cacatccccg tcaccgtgtc
     1261 agcgtgcgtg tttcccaacg gcgacataac gttgcattgc cccgccatcc aactgatcgg
     1321 gctctcgcat gcgaagtccg ccttcacgta ctgcggcaac gacaccggcg cgatcaggca
     1381 gctgccccgg cccctgttgg acgacctcga cgcctgcgcc cggaccgtcg gtcgctggct
     1441 gcatcgacgg ggctacgttg gcgcgttcgg ggtcgacgcg ctcgtgcatg aggggcgtct
     1501 cctgttcggt gaattgaatc cgcgcttcca gggctcgatc cgcatctcga atgcgctcga
     1561 cgccgccctg ggccggccgg acatcctgca agatcacctc atggcctggc tggggattcc
     1621 tgccatcccc tcgccgccgc tggccgaact cgtcgccgct cagccagctc gttcgcaggt
     1681 cctcttgttc aacaacagcg ctgcccgcac acgcgtgcgc aggcatgacg ccccgttgcc
     1741 cgagggagcg gagatgctgc tcgctcccgc ggagcacgta ctcgtcgatc ccggcgaact
     1801 cgccttcgtc ctcgagttcg agaggtcggt cactactgac ggacgcagtc tcgaggcgaa
     1861 catcgccgcg acggtacagg cctcgctcgc gaccgcgctt gagactcagg atgcccgccg
     1921 gcgcggacgg cgctgatcga cctccgagta gccgcctgag tgcagccccg gggcgcaatc
     1981 cgccgcgcaa accacgccga gcaggctcag ggtcgcctga gcccgcccgg cttcgcctct
     2041 cgcccgcgtc actgcgcgta gttgctcgcc ccgaaccagt ccacgacgac caccggctcg
     2101 tcgcctacca cccacgcgtc gtgccccgag ggcaacgcgg tcacgtcccc gggcacggcg
     2161 tcgaactcgc gtccgtcctc catcaggatc cgcagggtcc cggacacgtg gtactggaag
     2221 tgcggtgcct cgcagtactc ggtgtgcgcg atcggcttca cgtgctccga ccagcgccac
     2281 cccggctgca acgtcagccg ccccacctgc ccgcccccga tctcgatcag gtccacctgc
     2341 ccgcgttcaa acgtccgagt ctcgtccggg ctcccaaacc gcttctgctc tgccgatgcc
     2401 atcgttcgcg tcctctcccg gccgtctggc tgttcgtgtc gcggccgtcg aggacatcct
     2461 gtcgcgccga gcgagccccg ccatcaggag gatcgctcat ttccgcggcg ccgcctcggc
     2521 tgcgcaccgc gctcgtccgg tagtacgaaa tgactatcga gcagcccgcg gggccgcgct
     2581 caggcgagcc cgttgcgcag cgcaaacgcc gccgctgcgg cgcggttgtc cgcgtcgatc
     2641 ttcgtgaaga tgtgtgccag gtggttcgcc accgtccgct cgctgatgac cagcgccgcc
     2701 gcgatctcgc ggttcgtctt cccctccacg atcagccgca gcacctccac ctcgcgcgca
     2761 gagagccccg ctgggccgcc cgctcgcggc tccgcccgct ccagcaactc gaggcgtgtc
     2821 agctccccgc ccatcccgag ctcctccagc aggccacgcg cctcag
//
LOCUS       DAORPG010000209         2860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3628_length_2860_cov_2.999287, whole genome shotgun sequence.
ACCESSION   DAORPG010000209 DAORPG010000000
VERSION     DAORPG010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3628_length_2860_cov_2.999287"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PLX85_05530"
     CDS             <1..555
                     /locus_tag="PLX85_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62667.1"
                     /translation="LLHAGAAESLTEVVAAAGGRLVGRLVEAATAADTAAAQEAGAQF
                     LAFDDRRTEAAALLDQRIGYVALLGQEEDADLRLLRPLDLDGAVVASPSAQLTVRDQL
                     RLRRTADLVRKPLFARLSTAVDAATLEVWRDAGVVAVVVSDPALVSEVARAADAVPRP
                     RESTERREVVVPSVQVPADEDDED"
     gene            complement(597..1064)
                     /locus_tag="PLX85_05535"
     CDS             complement(597..1064)
                     /locus_tag="PLX85_05535"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRC62668.1"
                     /translation="MLEVGDRMPAFSLRNSSREEVTQDEFLGSVSVLAFYPMSFTGGC
                     TREMQGFQQKYEDFQALGVKIAGISGDTWATQGIFCEQHSLQFPVLSDWPKYETIAAF
                     GVDRPESPMARRVTFVFDADGVCRQVITEEREMDAHRDGALEIARTLAAGTAP"
     gene            complement(1126..1377)
                     /locus_tag="PLX85_05540"
     CDS             complement(1126..1377)
                     /locus_tag="PLX85_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62669.1"
                     /translation="MRIWSRVAGAGLGALLALLPIIASAEEGSRVLERGDDWNKVMIL
                     TFATLLGVMFLATLGFLYRMKRHLFWDFQQPAAPHDDHH"
     gene            1560..1694
                     /gene="rpmH"
                     /locus_tag="PLX85_05545"
     CDS             1560..1694
                     /gene="rpmH"
                     /locus_tag="PLX85_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRC62670.1"
                     /translation="MKRTWQPKRIPRLRKHGFRARMATRGGRDVLKRRRARGRARLTV
                     "
     gene            1708..2088
                     /gene="rnpA"
                     /locus_tag="PLX85_05550"
     CDS             1708..2088
                     /gene="rnpA"
                     /locus_tag="PLX85_05550"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRC62671.1"
                     /translation="MTAESAAAPPATVPPATRLTRRQDFLLVLQDGQRVRHPLLGFGF
                     RPNGLEQSRVGFAVGKRVGNAVTRNLVRRRLRAITRATALVPGYDIVVTARQNAAMAR
                     YGELEQAFRQCARRGTLLAAEAAR"
     gene            2043..2336
                     /gene="yidD"
                     /locus_tag="PLX85_05555"
     CDS             2043..2336
                     /gene="yidD"
                     /locus_tag="PLX85_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRC62672.1"
                     /translation="MRTPRHVAGGRGCPVRVVLLRAIRLYQRGISPALGARCRFEPSC
                     SEYAYQAIETHGAGRGAWLAARRLLRCRPGKPGGYDPVPPLEASLSHPKIVKN"
     gene            2351..>2860
                     /locus_tag="PLX85_05560"
     CDS             2351..>2860
                     /locus_tag="PLX85_05560"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRC62673.1"
                     /translation="MKHSALFALAGLVLIGLLTGCGRASKPQGWSAPVDAGGGIVIVH
                     DQRGSLTALQVTDAGSSALWTFPGSTDDRNYQGFYATPIVDRSGASPRLLVASYSGHV
                     VSVDLATGAATPGWPKEVSVGGHIVATPVLVGSTLYVANVHGDVKPVDITNGTVGQTV
                     AKASDRIWGA"
BASE COUNT          432 a          900 c         1064 g          464 t
ORIGIN      
        1 ttgctccatg ccggagccgc ggagtcgctg accgaggtcg tggccgcggc tggtgggcgc
       61 ctcgtagggc ggctggtgga ggccgcgacg gccgctgata cggctgctgc ccaggaggcg
      121 ggagcgcagt tcctcgcgtt cgatgaccgc cgcacagaag ccgccgcgct gctggaccag
      181 cggatcgggt atgtggcgct gctgggccag gaggaggacg ccgacctgcg cctgctccgc
      241 ccgctcgacc tcgatggggc agtcgtcgcg tcgccgtccg cacagctcac cgtgcgcgac
      301 cagctgcggc tgcggcgcac cgcggacctc gtccgaaaac cgttgtttgc acggctgtcg
      361 acggccgtcg acgccgcgac gctcgaggtc tggcgcgacg cgggcgtcgt ggccgttgtc
      421 gtctcggatc cggcgctcgt ctcagaggtt gccagggcgg cggatgcggt cccgcggccg
      481 cgcgagtcga ctgagcggcg cgaagtcgtg gtgccgtccg tgcaggtgcc ggctgacgag
      541 gacgacgaag actagtccgt ctccccagtg ttctcgtctc gatcagtgcg gccgggctag
      601 ggggcggtgc ctgccgccag tgtcctggcg atctcaaggg cgccgtcgcg gtgcgcgtcc
      661 atttctcgct cttcggtgat cacctggcgg cagacgccgt cggcgtcgaa gacgaaggtg
      721 acgcggcgcg ccatcggtga ctcgggccgg tcgacgccga aggcggcgat ggtctcgtac
      781 ttcggccaat ccgagagcac cgggaactgc aggctatgct gctcgcagaa gatgccctgc
      841 gtggcccagg tgtcgccgct gatgccggcg atcttcactc ccagcgcctg aaagtcctcg
      901 tacttctgct ggaacccctg catctcgcgg gtgcagccgc ccgtgaacga catggggtag
      961 aaagcgagga cggagaccga gccgaggaac tcgtcctggg tcacttcctc gcggctggag
     1021 ttgcgcaggg agaaggcggg catccggtcg ccaacttcga gcatgggtgt ggctcctcgg
     1081 tgtcagctgc agaccgcagt cagggaaggc gcacgggcgc ggcgactagt ggtggtcgtc
     1141 gtgaggcgcg gcgggctgct ggaagtccca gaagaggtgg cgcttcatgc ggtagaggaa
     1201 gccgagcgtg gccagaaaca tgacgccgag caaggtggcg aaggtgagga tcatcacctt
     1261 gttccagtcg tcgccgcgct cgaggacacg ggagccttct tcggcggacg cgatgatggg
     1321 aagcagggcg agcagggcgc cgagacccgc gccggcgact cgagaccaga tgcgcatggg
     1381 aacctccatc cgagagggag tctagcacgc gctggcgggg aggcacgagc gcgagggaag
     1441 gctgtgcact cgtcttcggc gaggggtgag cgatagcgat cgcgtatgtg ctcgcgttgt
     1501 ccctcgatgc ggggttccgt atactctgtc ggcatcccga ccatctaaag gagcgtccgg
     1561 tgaaacggac ctggcaaccg aagcgaatcc cccgtctgcg caagcacggg ttccgagccc
     1621 gcatggcgac gcgcgggggg cgcgacgtgc tcaagcgccg ccgggcgcgt ggacgggcac
     1681 gtctgaccgt ctagcggctc gtcgtttgtg acggccgagt cagcagcggc ccctcccgcg
     1741 accgtcccac ctgcaacgag gctgactcga cgccaggatt tcttgctggt gctccaggac
     1801 gggcagcgtg tgcggcaccc gctgctcgga ttcgggtttc ggcccaacgg gctcgagcag
     1861 agccgggtcg gtttcgccgt cggaaagcga gtcggcaacg cagtgacacg aaacctcgtc
     1921 cgccgccgct tgcgcgcgat cacacgggcg accgccctcg tgcccggata tgacatcgta
     1981 gtgacggccc ggcagaacgc ggcgatggcg cgatacggcg agctggagca ggcattccgg
     2041 cagtgcgcac gccgcggcac gttgctggcg gccgaggctg cccggtgagg gtggtgctgt
     2101 tgcgggcgat ccgcctgtac cagcgaggca tctcgccggc cctgggagcg cggtgccgct
     2161 tcgagccttc gtgttctgag tacgcatacc aggcgatcga gacgcatggc gcgggtcgag
     2221 gcgcatggct cgctgcccgg cggctgttgc gctgccgccc ggggaagccg ggcgggtacg
     2281 acccggtgcc accgctggag gcgtcgctct cccaccctaa gattgtgaaa aattgaacaa
     2341 aatgtttcac gtgaaacatt cggcgctgtt cgcgctcgcg ggcctcgtgt tgataggtct
     2401 gttgacgggc tgtgggcggg cgtcgaagcc gcagggctgg tctgcgcccg tcgatgccgg
     2461 cgggggcatc gtgatcgtgc acgaccagcg gggctcgctg acggccttgc aggtcacgga
     2521 cgccggatcg agcgcgctgt ggaccttccc tggcagcact gacgaccgca actaccaggg
     2581 gttttacgcg actccgatcg tcgaccgcag tggtgcctcg cctcgtctgc tcgtggcgtc
     2641 gtattccgga cacgtggtgt ccgtcgacct cgcgacgggg gcggccactc cggggtggcc
     2701 caaggaagtg tcggtgggcg ggcacatcgt ggcgacgccg gtgctcgtcg gcagcacgct
     2761 gtacgtggcg aacgtgcacg gcgacgtgaa gccggtggac atcaccaacg ggacggtcgg
     2821 gcaaacggtg gcaaaggcct ccgaccgcat ctggggcgcg
//
LOCUS       DAORPG010000210         2845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3664_length_2845_cov_4.236201, whole genome shotgun sequence.
ACCESSION   DAORPG010000210 DAORPG010000000
VERSION     DAORPG010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3664_length_2845_cov_4.236201"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PLX85_05565"
     CDS             complement(<1..645)
                     /locus_tag="PLX85_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HRC62674.1"
                     /translation="MSKRELGSEDSHRQAAPEGLPDPRSLAPKLAGLSPADRFRRLDS
                     TPSGLSSGEVRARRERDGWNDPVAPAARHRLVPLLRNFTHTLALLLWFAAGLALAARI
                     YELAFAVVAVIAVNGVFAAAQEYRAEQVVEGLMRRVAVRSRVLRDGTWQVLPARDLVR
                     GDVVALEKDIRAAAAKVGVQAEFTGEAEQAPPSSGGGELIGLAACTHERHLTVDE"
     gene            complement(680..1324)
                     /locus_tag="PLX85_05570"
     CDS             complement(680..1324)
                     /locus_tag="PLX85_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRC62675.1"
                     /translation="MTGQGSPAANTALLEAIVESAPDGILVVDSDARIHLANRQAETL
                     FQYPRDQLIGEPLHRLLPARYHASHLRAVDAFGRQPTMRPMGSGLHIVAQRRDGTEFA
                     ADIALSPVTTPTGSFVIASVRDVSSHQLLQQEHQQLSDRVRVHSERERAALGLQDSLI
                     QVSYGVGLNLMKAKELVGGSSSDAGQVLDEAIEELNNLILRVRRLILDISGAVE"
     gene            1570..2049
                     /locus_tag="PLX85_05575"
     CDS             1570..2049
                     /locus_tag="PLX85_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62676.1"
                     /translation="MSRSTLSLLVMAALLVIAVGAAALIASLRSGEDELPAGTPERTV
                     QLYLKAVEDHDATEAFSYLAPSLSARCASFPREEITQRGSSSIRATLDEVRVDGDRAT
                     VRVGLSETFGNAPFGTSDPKQSLTFELQQIEGQWRFTEAPWPLYCAPKLSSAGGLGR"
     gene            2071..>2845
                     /locus_tag="PLX85_05580"
     CDS             2071..>2845
                     /locus_tag="PLX85_05580"
                     /inference="COORDINATES: protein motif:HMM:NF039189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HRC62677.1"
                     /translation="MVVSLVPLLVIIGVVVMLIRRRQPREDGHPTSDTGLGTIRRLFL
                     YGIGLLALIFSGVGLSLLLGGLIDSFGGATVISESDTELAFALAFSIVGLPAWLIAAL
                     LAQRSLASAREAEVGSEVRRLYLNLARGIALMVVAVNAITVLQFVLRISDFDGRAVGW
                     LAVWLAIWLLHDLIVRGEHRTPATAWLDHLYLGFASVVGLLFLLSGLGAGLGSVLSAA
                     YDGLFRSTLVHGDWSRGLREAGAPALVGAMVWGVHWLLRL"
BASE COUNT          401 a          998 c          954 g          492 t
ORIGIN      
        1 ctcgtccacc gtgaggtggc gctcgtgcgt gcacgccgcc agcccgatga gctcaccgcc
       61 gcccgagctt ggcggtgcct gctcggcctc gcccgtgaac tccgcctgca cccccacctt
      121 ggcggccgcg gcgcggatgt ccttctcaag cgccaccacg tcgccgcgca cgaggtcgcg
      181 cgccggcagc acctgccatg tcccgtctcg gagcacgcgt gagcgcacgg cgacccgccg
      241 catcaggccc tcgacgacct gctcggcgcg atactcctgc gccgctgcga acacgccgtt
      301 caccgcgatc acggcgacga cggcaaacgc cagttcgtag atgcgcgccg cgagcgcgag
      361 cccggccgcg aaccacagca gcagcgcgag ggtgtgcgtg aagttgcgca gcagcggcac
      421 gaggcgatgc cgcgccgctg gcgccacggg gtcgttccag ccgtcacgct cgcggcgagc
      481 ccttacctcc cctgaggaca gcccgcttgg cgtcgagtcc agccggcgaa aacggtccgc
      541 agggctcaac cccgcgagct tgggggcgag gctgcgaggg tccggcaggc cctccggcgc
      601 tgcctgccgg tgggaatcct cggagccgag ctcgcgcttg ctcatcgctc gacgatacgc
      661 cgggcgcatg ggagacgagc tattcgacgg ccccggagat gtcgaggatt agccgccgca
      721 cccggaggat caggttgttc aactcctcga ttgcctcgtc gagcacctgc ccagcgtcgg
      781 agctcgaccc gccgacgagt tccttcgcct tcatgaggtt gaggcccacg ccatacgaaa
      841 cctggatgag cgagtcctgc aggcccagtg ccgcccgctc ccgctccgag tgcacgcgca
      901 cgcggtcgga aagctgctga tgctcctgct gcagaagctg gtgactcgag acgtcgcgca
      961 cggacgcgat gacgaaggag ccagtggggg tcgtcaccgg gctgagagca atgtcggcgg
     1021 cgaactccgt gccgtcacgg cgctgcgcga cgatgtgcag gccggagccc atgggacgca
     1081 tcgtgggctg gcggccaaac gcatccaccg cgcgaaggtg gctggcgtgg tagcgagccg
     1141 gcagcagccg gtgcaggggc tcgccaatga gctggtcacg ggggtactgg aatagcgttt
     1201 ccgcttgccg gttcgcgagg tgaatccgtg cgtcagagtc tacgacgagg attccgtcgg
     1261 gggcggactc tacgatcgcc tcgagcagag cggtattggc tgccggcgaa ccctgcccag
     1321 tcacgatgtc ccgccgacgc cttgaactct catgttgata cgccgaatca ttccgcgtgg
     1381 attcactgta ggtctgcgcc cttggaccgc cggagggccg aaggtcatcg accgttcggg
     1441 actgccatcg ccgacgccgg agggtgacga acgtcacctc gatgtccttc acaggggctc
     1501 tggcgcgctc tcgcgcactg atgaatcctg agcaggagcg gtctgccggt tgagtgtggg
     1561 gagtcatccg tgtcacggtc tacgctctcg ctcctcgtca tggccgcact cctcgtgatc
     1621 gcggtgggcg cagcggcgct gatcgcctcg ctccgctcgg gcgaggacga gctgcccgcg
     1681 gggacacccg agcggacggt gcagctctac ctgaaggccg tcgaggatca cgatgccacc
     1741 gaggccttct cctacctggc cccctcgctt tcggctcgat gcgcgtcgtt ccctcgcgaa
     1801 gaaatcacgc agcgcggcag ctcgtccatc cgcgcgacgc tggatgaggt gcgcgtcgac
     1861 ggcgaccgtg ccacggtgcg cgttggccta tcggagacgt tcggtaacgc tcccttcggc
     1921 acgagcgacc cgaaacagag ccttaccttt gaactccagc aaatcgaggg ccagtggcgc
     1981 tttaccgagg ctccctggcc gctgtactgc gcgccgaagc tgtcgtctgc cggcggactc
     2041 ggccgctgag tggctaggga tgtgatcggc ttggtcgtct cactcgtccc gctgttggtc
     2101 atcattggcg tcgtcgtgat gctcatccgg cggcgccagc cccgcgagga cgggcacccc
     2161 acttccgaca ccggcctcgg caccatccgg cgattgttcc tctacggtat cgggttgctc
     2221 gcgctcatct tctcgggcgt cgggctgtcg ctgctgctcg gtggcctgat cgactcgttc
     2281 ggtggcgcga ccgtgatctc cgaaagcgac acagagctgg cgttcgccct cgccttcagc
     2341 atcgtcgggc ttcccgcctg gctgattgcc gcgctcctcg cgcagcgctc gctggccagc
     2401 gcccgcgaag ccgaagtggg ctccgaggtg cgtcgcctct acctcaacct ggcccgtggc
     2461 atcgcgctca tggtcgtggc ggtcaacgcc atcacggtcc tccagttcgt gctgcggatc
     2521 tccgacttcg acggccgcgc cgtcggctgg ctggccgtct ggctggcaat ctggcttctg
     2581 cacgacctga tcgtgcgggg cgagcaccgg acgcccgcga cggcgtggct cgatcacctc
     2641 tacctcgggt tcgcctcggt ggtcgggctc ctgttcctcc tcagcggcct cggcgccggc
     2701 ctcggctcgg tgctctccgc cgcctacgac ggactcttcc gctcgaccct cgtacacggg
     2761 gactggagcc gagggctccg cgaggccgga gcgccagcgc tggtaggcgc gatggtctgg
     2821 ggcgtccact ggctgctgcg acttc
//
LOCUS       DAORPG010000211         2833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3703_length_2833_cov_3.029158, whole genome shotgun sequence.
ACCESSION   DAORPG010000211 DAORPG010000000
VERSION     DAORPG010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3703_length_2833_cov_3.029158"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="PLX85_05585"
     CDS             <1..941
                     /locus_tag="PLX85_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRC62678.1"
                     /translation="LASDQIYAMDYREMIATHVEADADITVSVADVPLHDASRYGLVT
                     MDATGRVRRFEEKPARPRSTLASMGIYVFNPDYLARTLAADAADETSTHDFGGDVLPR
                     AIAGGARVYAHRFRGYWRDIGTVEAYWKTHMELLDEPSRVPLRAPGREIMTRMDPRPP
                     AAIASSASVQQALVSAGATIRGTVRSSVVSPGVVVEPGAVVHDSVLLPGVRVGAGAIV
                     HRAVIDQDAVIGSAARIGADWYDASEHTEHELGVTVVGEGSWIPAGGIVEPSDVVAPF
                     SGRGAAPPRIAASGDEAAGASAWRGLSRGPLTALRP"
     gene            990..1874
                     /gene="otsB"
                     /locus_tag="PLX85_05590"
     CDS             990..1874
                     /gene="otsB"
                     /locus_tag="PLX85_05590"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HRC62679.1"
                     /translation="MAGPAESVAATTPPEHRDVTPIDALPSALEAFEAIRARVDGLTP
                     ILCLDFDGTLSPIVDDPASAVLLDGIEPVLRRLVRMTPILVLSGRDADDVRARVGVGG
                     ILCAGSHGFDVLDTDGQHEPNAEASAYLPHLDALEARLRTETQDIAGVVIDRKRFGVA
                     VHDRQVTDERDLERLWSLVSAAAEAFPMLRPLRGKRVTEFLPEIDWNKGRALLWLLGR
                     AGYRADAAVPICIGDDVTDEDALRAVREQGIGIVVGTGSRLTSASYAVRDPAEVLAFL
                     TQLADFLDVRVLPAVGVP"
     gene            1890..>2833
                     /locus_tag="PLX85_05595"
     CDS             1890..>2833
                     /locus_tag="PLX85_05595"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HRC62680.1"
                     /translation="MADGRLVVVSNRVLAAGGRVSVGGLVAALRPALEAVGGLWFGWG
                     GSIGPADAIERRREGRVEFVTCALTADEEEGYYEGFCNRTLWPLMHGMPERAELRAGE
                     YERWRAVNARFAALLRPFLEPGDLVWVHDYHLMALGSELRAAGWEGRAGYFHHIPVPE
                     AALWNAIPQHLEIAAGFEAYDLVGLQTQRDVARLERYAPAAAGRMRAFPIGIDPQGTR
                     ALAGQHPADAFEARTGRQVLLGLDRLDYTKGIPDRLTAFERLIRRRPEVAAEALLVQW
                     SAPSREGIADYQAERRRVDDAVGRLRALASPSPVEARH"
BASE COUNT          392 a          939 c         1061 g          441 t
ORIGIN      
        1 tcctcgccag cgaccagatc tacgcgatgg actaccgcga gatgatcgcg acgcacgtcg
       61 aggccgacgc ggacatcacg gtcagcgtcg ccgatgtgcc gcttcacgac gcctcgcgct
      121 acgggctcgt gaccatggat gcgacagggc gggtgcgacg gtttgaggag aaaccggcac
      181 ggccgcggtc caccctggcc tcgatgggca tctacgtctt caaccccgac tacctcgcgc
      241 ggacgctcgc cgcggatgcg gccgacgaaa cgagcacgca tgacttcgga ggcgacgtcc
      301 tgccgcgagc gatcgcgggc ggggcgcgcg tgtacgctca tcgttttcgc ggctactggc
      361 gcgacatcgg caccgtcgag gcgtactgga agacgcacat ggaactgctc gacgagcctt
      421 cgcgcgtgcc gctgcgcgct cccgggcgcg agatcatgac gcgcatggac ccgcgtccgc
      481 ctgcggcaat cgcgagctcg gcgtccgtgc agcaggcgtt ggtctcggcc ggggcgacca
      541 tccgtgggac cgtacggagc tcggtcgtct ccccgggggt ggtggtggaa ccaggggcgg
      601 tcgtgcacga ctcggtgctg ctgcccggcg tccgcgtggg tgcgggcgcg atcgtgcatc
      661 gggccgtgat cgaccaggac gccgtgatcg gctctgccgc tcggattggc gcggactggt
      721 atgacgcctc ggagcacacc gaacacgagc tgggagtcac ggtggtcggc gaaggcagct
      781 ggatccccgc gggcggaatc gtcgagccat cggacgtcgt ggctccgttc agcggccgcg
      841 gggcggcgcc tcctcggatc gccgccagcg gcgacgaggc ggccggcgcg agtgcgtggc
      901 gtggcctgtc gcgcggaccg ctgacggcgt tgcggccctg agccttgaat cacgaggtgc
      961 gcagcagact gacgcgctag acttcggata tggcgggtcc cgcagaatcc gtcgccgcga
     1021 ccacgccgcc cgagcaccgg gacgttacgc ccatcgacgc gctgccctcg gcactcgagg
     1081 cgttcgaggc gattcgcgcg cgggtcgacg gcctgacccc gatcctctgc ctcgacttcg
     1141 acgggacgct ctcgccgatc gtggacgacc ccgcgtccgc ggtactcctt gacggtattg
     1201 agccagtgct gcggcggctc gtgcggatga cgccgatcct cgtgctcagc gggcgcgacg
     1261 cggacgacgt ccgcgcacgc gtgggcgtgg ggggcatcct ctgcgcgggc agccacgggt
     1321 tcgatgtgct ggacaccgac ggtcagcacg aaccgaacgc cgaggcatcg gcatatctgc
     1381 ctcatctcga cgccctcgaa gcgcggctgc gcaccgagac acaggacatc gccggcgtcg
     1441 ttatcgaccg gaagcgcttt ggagtggcgg tgcacgaccg ccaggtcacc gacgagcggg
     1501 acctcgagcg tttgtggtct ctcgtgagcg ccgcggcgga ggcctttccg atgctgcggc
     1561 cgttgcgagg caagcgtgtc actgagttcc tgccggagat cgactggaac aagggccgcg
     1621 cgctgctgtg gcttctcggc cgggcaggct atcgagcgga cgcggccgtc ccgatctgca
     1681 tcggagatga cgtgacggac gaggatgcgt tacgggccgt gcgggagcag gggatcggca
     1741 tcgttgtggg gacgggcagc cggctcacgt cggccagcta cgccgtacgc gatccggccg
     1801 aggtgctggc attcttgacg cagctcgccg acttcctcga tgtgcgcgtg ctgccggctg
     1861 tgggcgtgcc gtgaccgaga ccgcgcacgt tggctgacgg ccgcctcgtt gtcgtctcga
     1921 atcgggtgct ggcggcggga ggtcgagtca gcgtcggcgg tctggtcgcc gcactgcggc
     1981 cggcactcga ggccgtcggg gggctctggt tcggatgggg tggctccatc gggccggccg
     2041 atgctatcga gcggcggcgc gaggggcgcg tcgagttcgt cacgtgcgcg ctcacggccg
     2101 acgaggagga aggctactac gaggggttct gcaaccggac gctgtggccg ttgatgcacg
     2161 gcatgccgga gcgcgccgag ctgcgggccg gcgagtacga gcgctggcgc gctgtcaacg
     2221 cgcggttcgc ggcgttgctg cggcccttcc tcgaacccgg tgacctggtg tgggtccacg
     2281 actatcacct gatggccctc gggtcggagt tgcgcgcggc cggctgggag ggccgcgctg
     2341 gctacttcca tcacatcccg gtgccggaag ccgcgctctg gaacgcgatc ccccagcacc
     2401 tcgagatcgc ggccggcttc gaggcctacg atctcgttgg attgcagacg cagcgggacg
     2461 tggcgcgcct cgagcggtac gcgccggcgg cggccggccg gatgcgcgcc tttccgatcg
     2521 ggatcgaccc ccaggggacg cgggcgcttg ctggccagca ccccgcggac gcattcgagg
     2581 cacgaacggg acgccaggtc ctgctgggcc tcgaccggct cgactatacg aagggaattc
     2641 ccgaccgcct gactgcgttc gagcggctga ttcgacggcg tcccgaggtg gccgcggagg
     2701 cgctgctcgt gcagtggtcc gcgccgtcgc gtgaaggcat cgcggactac caggccgagc
     2761 gacgccgcgt tgacgacgcg gtggggcgac tgcgggcgct cgcgagtccc tcgccggtcg
     2821 aggcgcgcca cga
//
LOCUS       DAORPG010000212         2831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3707_length_2831_cov_3.303674, whole genome shotgun sequence.
ACCESSION   DAORPG010000212 DAORPG010000000
VERSION     DAORPG010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3707_length_2831_cov_3.303674"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="PLX85_05600"
     CDS             <1..847
                     /locus_tag="PLX85_05600"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRC62681.1"
                     /translation="YAWMTDPAAEVEHMARELEEAKAAGVRTVIDVTTPDLGREAKLI
                     RRASEAAGVHVVVTTGIWLDIPRAFVRASVDDLAALFTREIEVSVGEADCRAGVIKVA
                     NAEPPGVGDLEERVLRAAARAALRTRVPITTHTGPYAIGREQMRVFADEGLPPYLAAI
                     GHSFTDDIAYLEEVLGRGHYLSMDHFGQGREHEARVIEAIARLCASGHAARIMLSHDH
                     VPEATLSNYWPWAGHLEHASPTRYSYVLREAVPALKRAGVSDADIDRMLIEAPATFLL
                     GGRGA"
     gene            complement(851..2281)
                     /locus_tag="PLX85_05605"
     CDS             complement(851..2281)
                     /locus_tag="PLX85_05605"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC62682.1"
                     /translation="MAAIETAREGGVDEAFPRAGTPSHAASSKRGWGGTFSALAERDY
                     AVYFTGNLAFFLAMQMDQLMRGLFALELTDGAWALGVVSVANAIPMLIVAPFGGVIAD
                     RYSKKKLLLWTQGFIAAVNVGFTVMIALDLVEFWHLLLGAFLQGVTFSVAMPVRNALV
                     PQLIPRHKLMNAVSLQMGGMNLTRIVGPALAGLLIAPLGLGPVWGIEATLFIVATLSI
                     LPLPSHGMAGRASSGEAMLSQLAEGFRYIGRAPLIRVLLLSALVMPLFAFPVQLVLPV
                     FALKVYDMGSSALGILMAFAGLGGLIGALLTASLDGAPHKGRIMLIGALLQGGAFIAF
                     ALTPVFPVALVLLAAGNIGGMLFMTTNNSVIQTRVPEEYRGRVMAVLMMSFGVMPLGV
                     LPMTLAADAIGAPAAVVISSSLMIATLAAFFTSSSNLRTLQVTPGRRAELSPAQAAAL
                     VAQGKISEEEAAHLTGMAESPAAGDA"
     gene            complement(2433..2663)
                     /locus_tag="PLX85_05610"
     CDS             complement(2433..2663)
                     /locus_tag="PLX85_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRC62683.1"
                     /translation="MPLYEYHCKKCDTQFERLRPASARGEATSCPSGHVGARPLLSLV
                     ASVSTMTRPIARDREEQPRAGGCACGGAGCGH"
     gene            2732..>2831
                     /locus_tag="PLX85_05615"
     CDS             2732..>2831
                     /locus_tag="PLX85_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62684.1"
                     /translation="MLGLPAGIRACLFDLDGVLTDTASVHAAAWKAT"
BASE COUNT          475 a          957 c         1007 g          392 t
ORIGIN      
        1 ctacgcctgg atgaccgacc cggccgccga ggtggagcac atggcgcgcg agctcgagga
       61 agcgaaagcg gctggagtcc gtaccgtcat cgacgtcaca acgccggacc tcgggcggga
      121 ggccaagctg atccggcgcg cctcggaggc ggccggcgtg cacgtcgtag tcaccacggg
      181 tatctggctg gacatcccgc gcgccttcgt gcgggcgagc gtcgacgacc tcgccgccct
      241 cttcacccgc gagatcgaag tgagcgtcgg tgaggccgat tgccgcgcgg gcgtgatcaa
      301 ggtcgcgaat gccgagccgc ctggcgtggg cgacctcgag gagcgggtgc tgcgggccgc
      361 ggcgcgggcg gcgctgcgca cccgcgtccc gatcaccacg cataccggcc cctacgccat
      421 cgggcgcgag cagatgcgag tcttcgcgga cgagggactg cctccgtacc tggcggcgat
      481 cgggcactcc ttcacggacg acatcgccta cctcgaggag gtgctcggcc gcgggcatta
      541 cctttcgatg gaccacttcg ggcagggccg cgagcacgag gcgcgtgtca tcgaggcgat
      601 cgcgcgactc tgcgcgtcgg gccatgccgc ccggatcatg ctgagccatg accatgtgcc
      661 ggaggcgacg ctgtcgaact actggccgtg ggcgggacac ctcgagcacg cgtctccgac
      721 gaggtactcg tacgtcctgc gcgaggccgt gccggcgctc aagcgggcgg gcgtcagcga
      781 cgccgacatc gaccgcatgc tgatcgaggc gccggccacg ttcctgctcg gaggccgcgg
      841 ggcctgagcg tcaggcgtcg cccgcagcgg gcgactcagc catcccggtg aggtgggccg
      901 cttcttcctc gctgatcttc ccctgcgcga cgagcgctgc cgcctgggcg ggcgagagtt
      961 cggcacgccg gccgggtgtc acctgcagcg tgcgcaggtt ggaactcgag gtgaagaacg
     1021 cggccagcgt ggcgatcatc agcgacgagg agatgacgac cgccgcgggc gcgccgatgg
     1081 cgtcggcggc gagcgtcatc ggcagcacgc caaggggcat cacgccgaac gacatcataa
     1141 gcacggccat gacgcgcccg cggtactcct cggggacgcg cgtctggatg acggagttgt
     1201 tcgtcgtcat gaagagcatg ccgccgatgt tgcctgccgc gagcagtacc agtgcgacgg
     1261 ggaagaccgg cgtcagggcg aaggcgatga acgccccacc ctgcaggagc gccccgatga
     1321 gcatgatgcg gcccttgtgc ggcgccccgt cgaggctcgc cgtcagcagc gctccgatca
     1381 ggccgccgag cccggcgaac gccatcagga tgccgagcgc cgacgagccc atgtcgtata
     1441 ccttcagcgc gaacaccggg agcacgagct gcaccggaaa cgcgaagagc ggcatgacga
     1501 gcgccgagag cagcagcaca cgaatcagcg gcgcccgccc gatgtagcgg aagccctcgg
     1561 cgagctgcga gagcatcgct tcgcctgacg aggcgcgacc ggccatgccg tgcgacggca
     1621 ggggcaggat cgagagcgtg gccacgatga acagcgtcgc ctcgatgccc cacaccggtc
     1681 cgaggccgag cggggcgatc agcaaccccg cgagcgcggg cccgacaatg cgcgtaaggt
     1741 tcatcccgcc catctggagc gagacggcgt tcatgagctt gtggcgcggg atcagctgcg
     1801 gcaccagggc gttgcgcacc ggcatggcca cggagaacgt aaccccctgg aggaaggcgc
     1861 ccaggagcag gtgccagaac tcgacgaggt cgagcgcgat catcaccgtg aacccgacgt
     1921 tgaccgccgc gatgaagccc tgcgtccaga gcagcagctt cttcttgctg taacggtcgg
     1981 cgatgacgcc tccgaatgga gcgacgatca gcatcgggat ggcgttcgcg accgacacca
     2041 cgcccagcgc ccaggcgccg tcggtcagct cgagggcgaa gagccctcgc atgagctggt
     2101 ccatctgcat ggcgaggaag aatgcgaggt tcccggtgaa gtacacggcg tagtcgcgct
     2161 cggcgagcgc gctgaaggtg ccgccccagc cgcgctttga gctggcagcg tgcgacggcg
     2221 tgccggcgcg cggaaacgcc tcgtccaccc cgccctcgcg agcggtctca attgctgcca
     2281 tgcagactcc ggtcgcgcag cccccgaccg gggactacag aagtgatgca gcgatactac
     2341 cgcacatcgg gccgcgcctg tgctggcggc cgatgacggt ttcgtggcag ctgtatcggc
     2401 agcatgaggg cgacacgcgc cgggaagaac ggttagtgcc cgcagccggc tccgccgcag
     2461 gcgcagccac ccgccctggg ctgctcctcg cggtcgcgcg cgatgggcct ggtcatcgtg
     2521 gacaccgagg cgaccagcga cagcagcgga cgagcgccga catgaccgct tgggcaggag
     2581 gtcgcttcgc cgcgcgcgct tgcgggccgc aggcgctcga actgggtgtc gcacttcttg
     2641 cagtggtact cgtacagcgg catacgtcct ccttcggcgt cgataatacg agatcggacg
     2701 gcggcattac caagcgtgct agggtggaat cgtgctcggc cttcccgcgg gcatccgcgc
     2761 ctgcctcttt gacctcgacg gagtgctgac ggacacggct tcggtgcacg ccgctgcctg
     2821 gaaggccacc t
//
LOCUS       DAORPG010000213         2830 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3710_length_2830_cov_3.259820, whole genome shotgun sequence.
ACCESSION   DAORPG010000213 DAORPG010000000
VERSION     DAORPG010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3710_length_2830_cov_3.259820"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PLX85_05620"
     CDS             <1..360
                     /locus_tag="PLX85_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRC62685.1"
                     /translation="VLAGEEVTARTARRIGLITDVVPHEELLPRTLDLANRVASMPRS
                     FVRVSKSEIDGTIDMMGWAANQALSRRMRPLRRGTPDDAFGPDGRTLTQIREQDGMQA
                     FIEARDAAWKERWTRDD"
     gene            complement(460..1278)
                     /locus_tag="PLX85_05625"
     CDS             complement(460..1278)
                     /locus_tag="PLX85_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRC62686.1"
                     /translation="MAVQNFELVSYEKRNRVAWLTINNAERMNALSTGVQNGLFYAFE
                     EADNDDDVLVVVLTGGGGRAFCAGADLREMTERDQTIGQGAAQQAKSGLSRAGETLKP
                     VIAAVDGYALAGGMQLSARCDFRIATEKSVFGMPEPLRSLTPINLMDTLELGFVPLGW
                     ALWIILSGDHISAQTAHDIGFVQWLVPDRDALMEEAEKRAEILKKCAPLAVQALKNGV
                     HLHMNPPTPPQGTLLLDHLKSVNAPLLDKVANSEDRMEGPKAFAEKRAPNWKGR"
     gene            1569..1823
                     /locus_tag="PLX85_05630"
     CDS             1569..1823
                     /locus_tag="PLX85_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HRC62687.1"
                     /translation="MDSGMIGKVAKAHRYAEERDRFQVTGLQVTVRGENSDHTVALDG
                     EHWHCTCDFFEHHGACAHTMALEIMFEGMLPQSVTFAMAS"
     gene            complement(1870..>2830)
                     /locus_tag="PLX85_05635"
     CDS             complement(1870..>2830)
                     /locus_tag="PLX85_05635"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRC62688.1"
                     /translation="NPVGLLATGDYIPARCAWSSALAAGGPLHIFDAPGMRDLLRRAD
                     LTVVPLEVGLMSSNQATPCERTTELQGAASAVDALVDAGVDVVTRASNHALDCYGGCS
                     GVLVKQETDAVLQRAGIASQGVGNSTAETSTALFVERDGVRFAILAYDDIAPWYHSTP
                     GGVGTGGLDLETLAADVRAAKARADHVIVAMHAGIEYQAEPTDRQRDAAHIAIEAGAS
                     LVIGNHPHWVQATERITSASGREGFVVYALGNFVFDQPWSTETQQGMLLEAGFTRERI
                     IGVRLRPHMIRDSYRPTLLNPATEGAPILERVWTATDGLAGGR"
BASE COUNT          409 a         1080 c          892 g          449 t
ORIGIN      
        1 gtccttgccg gcgaagaggt caccgcccgc accgcccgcc gcatcggcct cattaccgac
       61 gtcgtccccc acgaggagct gctgccgcgc acgctcgacc tcgccaaccg cgtcgcgagc
      121 atgccgcgca gcttcgtccg cgtcagcaag tccgaaatcg acggcaccat cgacatgatg
      181 ggctgggccg ccaaccaggc cctatcgagg cgcatgcgcc ccctccgccg cggcaccccg
      241 gacgacgcct tcggccccga cggccgcacc ctcacccaga tccgcgaaca ggacggcatg
      301 caggccttca tcgaggcccg agacgccgcc tggaaagaac gctggacccg cgacgactga
      361 gtgaccgcgc cacgcacaca aaacgaggcc gctccttgtg gagcggcctc gtgctacatc
      421 cgtgcccgcc gaggcgccgc cccggctcga ggttcggggt tagcggccct tccagttcgg
      481 ggcgcgcttc tcggcgaacg ccttcgggcc ctccatgcgg tcctcgctgt tcgcgacctt
      541 gtcgaggagc ggcgcgttga cgctcttcag gtggtcgagc agcagcgtgc cctgcggcgg
      601 cgtgggcggg ttcatgtgca ggtggacgcc gttcttcagc gcctgcaccg ccagcggcgc
      661 gcacttcttc aggatctccg cccgcttctc cgcctcttcc atcagggcgt cgcggtccgg
      721 caccagccac tgcacgaagc cgatgtcgtg cgccgtctgc gccgagatgt ggtcgcccga
      781 gaggatgatc cacagcgccc agcccagcgg cacgaagccg agctccagcg tgtccatcag
      841 gttgatcggc gtcaggctgc gcagcggctc cggcatcccg aagaccgact tctccgtcgc
      901 gatgcggaag tcgcagcgcg ccgagagctg catgccgccc gcgagggcgt agccatcgac
      961 ggccgcgatc accggcttca gcgtctcgcc ggcgcggctc aggccgctct tcgcctgctg
     1021 ggcggcgccc tgaccgatgg tctggtcgcg ctccgtcatc tcgcggaggt cggcgccggc
     1081 gcagaaggcg cggcctccgc cgcccgtgag caccacgacg agcacatcgt cgtcgttgtc
     1141 agcctcctcg aacgcgtaga agagcccgtt ctgcacgccg gtgctcagcg cgttcattcg
     1201 ctcggcgttg ttgatcgtca gccacgcgac gcgattccgc ttctcgtagc tcaccagttc
     1261 aaagttctgt acggccatct cgactccctt cctgagttca agtgcctcga ccaagcgacg
     1321 gcagtctagc acgtccgtca tccgctgcgc gggcgcgtct cgcgacgtcg caccgccgcc
     1381 gttcaccctc gtgtcgcccc gcagatcaag agcccttcga cacgttgacg agcccgttga
     1441 gcgaggacta ggctcggcac gtaggaggtc gtcatgagcg cacctcgctc cgttgctctc
     1501 cagaagcgcc gcgccgcaac gcggcgcttc ttgtttccag cgcgtgtcgt tcttggatgg
     1561 gaggcagcat ggactccggg atgatcggca aagtcgcgaa ggctcaccgg tacgccgagg
     1621 agcgcgaccg cttccaggtc accggcctgc aggtcacggt ccgcggcgag aacagcgacc
     1681 acacggtcgc cctcgacggc gagcactggc actgcacatg cgacttcttc gaacaccacg
     1741 gcgcctgcgc ccacacgatg gccctcgaga tcatgttcga gggcatgctt ccgcagtcag
     1801 tgaccttcgc catggcgtcg tagccggccg gccctgcggc gctggcctcg aggctccacc
     1861 cgctcgaggt cagcgcccgc cggcaagccc gtccgtcgcc gtccacaccc gctcgaggat
     1921 cggcgcgccc tccgtcgccg ggttgagcag agtcgggcgg tagctgtcgc ggatcatgtg
     1981 cggccgcagc cgtacaccga tgatccgctc ccgggtgaag cccgcttcca gcagcatccc
     2041 ctgctgcgtc tccgtcgacc acggctggtc gaacacgaag ttgccgagcg cgtacacgac
     2101 gaatccctcc cgcccggacg ccgaggtgat gcgctcagtc gcctgcaccc agtgcgggtg
     2161 gtttccgatc acgaggctcg cccccgcctc gatggcgatg tgcgccgcgt cacgctggcg
     2221 gtccgtcggc tccgcctggt actcaatccc cgcgtgcatc gccacgatga catggtccgc
     2281 gcgcgccttc gccgcccgca catctgcggc cagcgtctcg aggtccagcc cgcccgtccc
     2341 caccccgccc ggcgttgagt ggtaccacgg cgcgatgtcg tcatacgcga ggatcgcgaa
     2401 ccgcacgccg tcccgctcca cgaacagggc tgtgctcgtc tccgccgtgg agttgcctac
     2461 gccctggctg gcgatcccgg cccgctgcag cacggcgtcc gtctcctgct tgaccagcac
     2521 ccccgagcag ccgccgtagc agtcgagcgc gtggttcgag gcgcgcgtca ccacgtccac
     2581 gcccgcgtcc accagcgcat cgacggcgct cgccgcgccc tgcagctccg tcgtccgttc
     2641 acatggcgtc gcctggttgc tgctcatcag ccccacctcg agcggcacga cggtcaggtc
     2701 cgcccggcgc agcaggtccc gcatcccggg cgcatcgaag atgtgcaacg gcccgcccgc
     2761 ggccagcgcg gacgaccagg cgcatcgcgc cgggatgtag tcacccgtcg cgaggagtcc
     2821 gaccgggttg
//
LOCUS       DAORPG010000214         2822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3734_length_2822_cov_3.560896, whole genome shotgun sequence.
ACCESSION   DAORPG010000214 DAORPG010000000
VERSION     DAORPG010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3734_length_2822_cov_3.560896"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..1641)
                     /locus_tag="PLX85_05640"
     CDS             complement(154..1641)
                     /locus_tag="PLX85_05640"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC62689.1"
                     /translation="MTQQDPRTTDLATSRPIRQRPEPKEIDRKARFKVPSQLPAKQAP
                     AERIHNWDEVTFLFDPEIAKIEATRCIQCPAAPCQRACPVHNDIPGSFWLLEQGDFAA
                     AADVFRETSALPEMCGRLCPQERLCEGHCVVGKNGPPVAIGKLETFVTEWQRKHASVD
                     VTPVASASGFSVAVVGTGPAGIGCAERLARAGHRVVMYEAWPRPGGLLLYGIPNFKLN
                     KPAVARKFDDLSHLGVEMVTGTRIGEALSLDELANEFDAVFLAFGAPESAHLGIPNEE
                     APGVHEATEFLVRANLPREALPEQMREPLPELRQVVVIGGGDTSMDCVRSAIRLGAEA
                     VTLIYRRTETEMQGRVEERKHAREEGVQFEFLTMPVAVLTDESGAVSGLRCRRMQLGE
                     PDESGRARPEPIEGSDFDLPANAIVVAIGYNADPSWGDSAPEVRRDRWGRVVVDPESM
                     RSSQRGVFAGGDNVNGADLVVTALADGQKAAAAIELYLADPERSW"
     gene            complement(1830..>2822)
                     /locus_tag="PLX85_05645"
     CDS             complement(1830..>2822)
                     /locus_tag="PLX85_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRC62690.1"
                     /translation="KEAPRFNSISVSGYHIRDAGATAAQEIGFSFANAIEYISECERR
                     GANIDDVAPRISWIFNTHNYFFEEVAKYRALRRMWARLMKERFGAQQAESMMFRTHTQ
                     TGGSTLTAQQPLNNIVRASIQSLAAVVGGVQSLALSCYDEALSIPTEQAQRMALRTQQ
                     IIAHETGAADTIDPLAGSYYVEWLTNELEREAQAIIDRVDDLGGSVAAIEGGYMQSEI
                     QEAAVAYQREIESGERVIVGVNDFVDVEDQPQIIFRPNTTVGAEQVARLAKVRAGRDQ
                     GAVAASLARLREAARGDEPLMPPILEAVRTYATIGEMCGVLRDEWGEYTPPTVV"
BASE COUNT          424 a         1013 c          929 g          456 t
ORIGIN      
        1 caagtgcccg cagcctcccc cgtcgccgcg agatacacgc ggacgtcctc gagggtcacg
       61 gtgaacgcga tgggcggcag ccggtcgccg cgcctgagcg cgtggaagtc gatgctcact
      121 ggccgcgatt atccggggca ggccggtgcg gcgctaccag cttcgttccg ggtcggccag
      181 gtagagctcg atcgcggcgg cggccttctg gccgtcggcg agcgcggtca cgacgaggtc
      241 cgcgccgttg acgttgtcgc cgccggcgaa cacaccccgt tgcgaactgc gcatcgactc
      301 cgggtcgacg actacccgcc cccagcgatc ccggcggacc tccggagcgg agtcgcccca
      361 gctcgggtcg gcgttgtagc caatcgccac gacaatggcg ttggccggga ggtcgaagtc
      421 cgagccctcg atcggttcgg gccgcgcccg gccgctctcg tccggttcgc cgagttgcat
      481 gcgacggcag cgcagcccgg agaccgcccc ggattcgtcg gtcagcacgg cgaccggcat
      541 cgtgaggaac tcgaactgca cgccttcctc gcgagcgtgc ttccgctcct ccacccggcc
      601 ctgcatctcg gtctccgtgc gacggtaaat cagcgtcacc gcctcggcgc cgaggcggat
      661 cgccgaccgc acgcagtcca tcgaggtgtc gccaccgccg atgaccacga cctgtcgcaa
      721 ctccggcagc ggctcgcgca tctgctccgg cagcgcctcg cgaggcaggt tcgcccgcac
      781 gaggaactcg gtcgcctcgt gaacccccgg cgcctcctcg ttcgggatgc cgaggtgggc
      841 gctctcgggc gcgccaaacg ccaggaagac cgcgtcgaac tcgttcgcca gttcatcgag
      901 tgaaagcgcc tcaccgatgc gggtgccggt caccatctcc acgccgaggt gcgacaggtc
      961 atcgaacttg cgagcgacgg ccggcttgtt cagcttgaag ttcggaatgc catacaacag
     1021 cagcccgccg ggccgcggcc atgcctcgta catcaccacg cgatgcccgg cgcgcgcgag
     1081 gcgctcggcg cagccgatcc cggccggccc cgttcccacg actgccacgc tgaacccgct
     1141 cgccgaagcg acaggcgtca cgtccaccga ggcgtgcttg cgctgccact cggtcacgaa
     1201 cgtttcgagc ttgccaatcg cgaccggggg cccattcttg ccgaccacgc agtggccttc
     1261 acacagccgc tcctgcggac agagccggcc gcacatctcg ggaagcgcgc tcgtctcgcg
     1321 gaacacgtcc gccgcggccg cgaagtcccc ctgttcgagt agccagaacg atcccggaat
     1381 gtcgttgtgc accgggcacg cccgctggca cggggccgcg gggcactgga tgcagcgcgt
     1441 ggcctcgatc ttcgcgatct cgggatcgaa gaggaacgtc acctcgtccc agttgtggat
     1501 gcgctccgcg ggcgcctgct tcgccgggag ttgcgagggc accttgaacc gtgccttgcg
     1561 gtcgatctct ttcggttcgg ggcgctgacg gatcgggcgt gaggtagcga ggtcggtcgt
     1621 cctcgggtcc tgctgagtca tgtctgccgc cgttacagct gctcgaaaca cgcgtatgtg
     1681 aacttctgaa cgagcgctat gtctagggta tgtctgggcc gaaggcggct caaccctgag
     1741 ggtaacgagg catgcccggc gagcgccccc gaacgtcgca ggaagcggcg actcggcccc
     1801 gcacaggaac gtcgaggctt ggcgaaggct tacaccaccg tcggtggcgt gtactcgccc
     1861 cactcgtcac gcagcacacc gcacatctcg ccgatggtcg cgtacgtgcg caccgcttcg
     1921 aggatcggcg gcatcagcgg ctcgtcccca cgggccgcct cgcgtagtcg ggccagcgag
     1981 gcggccacgg cgccctggtc gcgccccgcc cgcaccttcg cgaggcgcgc gacctgctcg
     2041 gcgcccacag tggtgttcgg ccggaagatg atctgaggct ggtcctcgac gtccacgaag
     2101 tcgttcacgc cgacgatgac gcgctctccc gactcgatct cgcgctggta ggccaccgcg
     2161 gcctcctgga tctcggactg catgtagccg ccctcgatcg ccgccaccga cccgccgagg
     2221 tcgtcgacgc ggtcgatgat cgcctgcgcc tcacgctcga gctcattcgt cagccactcg
     2281 acgtagtacg agcccgccag cgggtcgatg gtgtcggcgg ccccggtctc gtgcgcgatg
     2341 atttgctgcg tgcgcagcgc catccgctgc gcctgctcgg tggggatcga gagcgcctcg
     2401 tcgtagcagg acagcgccag cgactgcacg ccgccgacca cggccgcgag cgactggatc
     2461 gaggcgcgca cgatgttgtt cagcggctgc tgcgccgtca gcgtcgaacc gcccgtctgt
     2521 gtgtgcgtgc ggaacatcat cgactcggcc tgctgagcgc cgaagcgctc cttcatcagc
     2581 cgcgcccaca tgcgccgcag cgcgcggtac ttcgcgactt cctcgaagaa atagttgtgc
     2641 gtgttgaaga tccaggagat gcgcggggcg acgtcgtcga tgttcgcgcc gcgccgctcg
     2701 cactccgaga tgtactcaat ggcattcgcg aacgagaacc cgatctcctg cgccgccgtc
     2761 gccccggcat cgcggatgtg gtagccggac accgagatcg agttgaaccg cggcgcctct
     2821 tt
//
LOCUS       DAORPG010000215         2817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3743_length_2817_cov_3.732078, whole genome shotgun sequence.
ACCESSION   DAORPG010000215 DAORPG010000000
VERSION     DAORPG010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3743_length_2817_cov_3.732078"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2251
                     /locus_tag="PLX85_05650"
     CDS             <1..2251
                     /locus_tag="PLX85_05650"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyketide synthase dehydratase
                     domain-containing protein"
                     /protein_id="HRC62691.1"
                     /translation="MLSSGMIAKDEVAALLAKLEPGGLEVPDVVFLAIGAGVEVASRI
                     IAELDACHISHDNCAHQSIVCGPAEQIEVVKDRLREQRVLFEELPFRSGFHTPAFAPF
                     VSEHVEHLRTLHLQRPDVTLWSATDCAPYPEAPADIQHLFARHLTEPVRFRELVTRLW
                     ESGVRVFVQVGAGSLHSFVDDTLGALPHNSLTGLAAQRSGLDQLRRVAAALFVEGANL
                     ELQRLGLAARTPVRAAPMKVALGAPLIRIDGASNVTPAPLAARAPEAPVQRTETVVLS
                     VDTYPELRDHCLFRQPPDWPELADRAPTVPMTLSIDLMREAASKLAPGKLAVGLEQVV
                     ASSWLHVAPPVEVTITATVVAPDRVRVSIGEYVSGTVVMGDRFPPAPATRRAVKNADP
                     VLFTAETIYRKRWMFHGPAYQGLASVEASDASGIRGTLINLPARGALLDATGQLASLW
                     IMQKAETDRVSLPVKVGGIQFFGPEPPADARVECEVLVRRFGQREVVSDITCWYEGAP
                     YCSITGWEDWRFDTGRGLYDLMLFPERNILAEVQPEGFVTIPVEQQTISALTSLARRF
                     LPAAAVEEMKSLRSPRHQAEWLYSRIAAKDAVRSYLFERGSGPIYPIEIAIEHDPQGK
                     PTVRSRTGRDMRVSISHTDGMAAAIAAEGVDPGIDVELIAPRGERFASIAFGAAELEQ
                     LPDPTSDEWLTRLWCAKEAAGKARGTGLEGSPRTLPLRAVEGTRFLIDGTWVRTRRIG
                     QYVIGWTDA"
     gene            2248..2553
                     /locus_tag="PLX85_05655"
     CDS             2248..2553
                     /locus_tag="PLX85_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRC62692.1"
                     /translation="MMEPSNNKQAILEEVGSMIRQVIGEEWIQNTPITMDTTFSEDLE
                     LESIEIVALAEQVQTRFGDEVDFPGWFGTLDVDAIIGLTVGQLVDRIAERTSATGVA"
     gene            2550..>2817
                     /locus_tag="PLX85_05660"
     CDS             2550..>2817
                     /locus_tag="PLX85_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC62693.1"
                     /translation="MSELEDRVALITGGASGIGLATAERFATHGAAVVIADLASSPGE
                     ARAAELRGRGLRASFIPVDVTNEDEVRAAFAQAAAEFGGVHLVVA"
BASE COUNT          478 a          883 c          989 g          467 t
ORIGIN      
        1 gatgctgtcc tccgggatga tcgcaaagga cgaggtcgcg gcgctcctcg ccaaactgga
       61 accgggcgga ctcgaggtgc cggacgtcgt gttcctcgcg atcggcgccg gcgtcgaggt
      121 ggcctcgagg atcattgcag agctcgatgc gtgccacatc tcacacgaca actgcgcgca
      181 ccaatcgatc gtctgcggcc ccgccgagca gatcgaggtc gtgaaggatc gattacgcga
      241 gcagcgcgtc ctgttcgaag agctgccgtt ccgatcgggg tttcacacgc ccgcgttcgc
      301 tcccttcgtg agcgagcatg ttgagcacct gcggaccctg cacctgcaac gccctgacgt
      361 tacgctgtgg tcggcaacgg actgcgctcc atacccagaa gctccggcgg acatccaaca
      421 cctgttcgca cggcacctca cggaaccggt gcgctttcgt gagctcgtga cgcggctctg
      481 ggagtccggc gtccgagtct ttgtgcaggt cggcgcggga agcctgcaca gcttcgtcga
      541 cgacacgctc ggggcgctgc cgcacaactc gttgaccggc ctggcggcgc aacggagcgg
      601 cctcgaccag ttgcggcggg tggcggccgc gttgtttgtc gagggggcca atctcgaact
      661 gcagcggctc gggctcgcgg ctcggacgcc tgtgcgagcg gcgccgatga aggtcgcgct
      721 tggagcaccc ctgatccgga tcgatggcgc ttcgaacgtg acgcctgcgc cgctggctgc
      781 gcgggcgccc gaggcaccgg tgcagcgcac cgagacggtc gtgctgtcgg tcgataccta
      841 cccggagctg cgcgaccact gccttttccg ccagccgcct gattggcctg agctcgccga
      901 tcgagccccc acggtgccga tgacgctgtc catcgacctc atgcgtgagg ccgcgagcaa
      961 gctggcgccg gggaagctgg cggtgggcct cgagcaggtc gttgcgagct cgtggctgca
     1021 cgtggcgccg ccggtcgagg tgacgatcac ggcgactgtc gtagcgcccg accgggtgcg
     1081 cgtgagcatc ggagagtacg tcagcgggac tgtcgtgatg ggcgaccggt tcccgcccgc
     1141 accagccaca cggcgcgcgg tgaagaacgc cgacccggtg ctgttcaccg ccgagaccat
     1201 ctatcgcaag cgatggatgt tccacgggcc cgcgtaccag ggactggcat cggtcgaagc
     1261 ctcggacgcg agcgggatca gggggacatt gatcaacctg ccggcgcgcg gagcgctcct
     1321 cgatgcgacc ggccagcttg cgagcctgtg gatcatgcag aaggcggaga cggaccgcgt
     1381 gtcattgccc gtcaaagtcg gtggcatcca gttctttggc cccgagcctc cggccgacgc
     1441 ccgcgtggag tgcgaagtcc tcgtgcgccg gttcggtcag cgcgaggtgg tgtcggacat
     1501 cacgtgctgg tacgagggtg ctccgtactg ctcgatcacg ggctgggagg actggcgctt
     1561 tgacacgggc cgcgggctct acgacctgat gctgttcccg gagcggaaca tcctcgccga
     1621 ggttcagcca gaggggtttg tcaccatccc ggtggagcaa cagaccatca gtgcgctgac
     1681 gtccctggcg cgtcgattcc tgccagcagc cgccgtggag gagatgaagt ccctgcgaag
     1741 cccgcggcac caggcggaat ggttgtatag ccgtatcgcg gcaaaggacg cggtgcgctc
     1801 atatctattc gagcgcggct cgggtcccat ctacccgatc gagatcgcga tcgagcacga
     1861 tccgcaggga aagccgaccg tgcgaagcag gacaggccgc gacatgcgcg tgtccatttc
     1921 gcataccgat gggatggccg cggccatcgc ggccgaggga gtcgacccgg ggatcgacgt
     1981 cgagctgatc gcgcctcgag gggagcgatt tgcatcgatc gccttcggcg ccgcggagct
     2041 ggagcaactg ccggacccga ccagcgacga atggctgaca cggctgtggt gcgcaaagga
     2101 agcggctgga aaggcgcgag gcactggcct cgaaggatcg cctcgaacgt tgccgctgcg
     2161 cgcggttgag ggcacacggt ttctcatcga tggcacgtgg gtgcgaacgc gccgcatcgg
     2221 acaatatgtg atcggttgga cggacgcatg atggaaccca gcaacaacaa gcaggcgatc
     2281 ctcgaggaag ttggctcgat gatccggcag gtgatcggcg aggagtggat ccagaacact
     2341 ccgatcacga tggacacgac gttctcggaa gatctcgagc tcgaaagcat tgaaatcgtg
     2401 gcacttgccg agcaggtcca aacgcgattc ggcgatgagg tcgattttcc ggggtggttt
     2461 gggacgctcg acgtggacgc gatcatcggt ctcaccgtcg gccaactggt ggaccgaatc
     2521 gcggaacgca cctcggcgac cggggtggcg tgagcgaact cgaggaccgt gtcgcgttga
     2581 tcacgggcgg cgcgtccgga atcggccttg cgacggccga acggttcgcg acgcacggtg
     2641 cagccgtggt gatcgccgac ctggcatctt caccgggtga ggcgcgcgcg gccgagctgc
     2701 gaggtcgcgg gttgcgcgcc tcgttcatcc ccgtcgacgt gacaaacgag gacgaagtgc
     2761 gagcagcctt tgcgcaggcc gccgcagagt tcggcggcgt ccacctcgtc gtcgcga
//
LOCUS       DAORPG010000216         2804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3769_length_2804_cov_3.587850, whole genome shotgun sequence.
ACCESSION   DAORPG010000216 DAORPG010000000
VERSION     DAORPG010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3769_length_2804_cov_3.587850"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..619
                     /locus_tag="PLX85_05665"
     CDS             203..619
                     /locus_tag="PLX85_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62694.1"
                     /translation="MPEIEHTPRPPRGRGGQPGNLNAFKDGRYSRRLGHAFRMAPPGE
                     MRDHVFAVFAVVVRLELARMGPHPTVEERVAAIERAIRRLHRAGTSRSYRGGRSPQEV
                     LRWFHDAINTALTATPEPIEPAPPAPRQTPENEGNE"
     gene            complement(677..>2804)
                     /locus_tag="PLX85_05670"
     CDS             complement(677..>2804)
                     /locus_tag="PLX85_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HRC62695.1"
                     /translation="EDEDESRDALERERLEEVRALRVRAWFALSVAAFLMVGMQWHRV
                     PVLDGIPARVMHPLFFALATPVQFWAGWRFYAGAWRVGRHGSADMNTLIAFGTTVAYA
                     YSVVATFFPGAFDAVGGHAHTGAAGHIQFEVTTYFDTSTAIIALVLLGRLLEARAKGQ
                     TSQAVRALIALRPRLARVIEDGQEYEIPLSAVQPGDLVVVRPSEQVPVDGEVVSGGSA
                     VDESMLTGEPLPVPKGPGDSVFGATMNSTGMLQVRATAVGADSALARIIRLVEEAQGS
                     KAPIQRVADSIAAVFVPVVIAIALATFAAWWAFGPEPAFTLAVLNAVTVLIIACPCAL
                     GLATPTAIMVGSGLGARHGVLIRDAQALEVARRVDTVVLDKTGTVTEGRPSVVGVLAA
                     TGGLNEDELVRLVASAERGSEHPLASAVVAEAERRGTDLEWPDTFRALVGQGIEATFG
                     GRTLLVGNRELLAERGVIAAELEREATERAVVGQTSLLVAVDGRAAGLIAVADPVRAT
                     SAGAIARLQAMGVEVVMLTGDQQATAEAVASQVGVQRVIAGVRPEGKSEVVRELQAQG
                     RHVAMVGDGINDAPALAAAEVGIAIGTGTDVAMEAAPITLMRPDLGGVATAIAVSRAT
                     MRTMHQNLAWAFGYNVVLIPVAAGLGYLVFHEALGTAVPLVLQPVFGEQGFLNPIVGA
                     LAMAISSVSVMTNSLRLRRARVDSFR"
BASE COUNT          424 a         1152 c          840 g          388 t
ORIGIN      
        1 ggtcgcgcgc tcgcgggccg ctccgcccgt gagggcgcgc ggctcggccc gggtttcggc
       61 gctgtcctgg tctgcccacc gatccagtgt agcccgcgct cgacgccgct ctctgccacg
      121 ccgcgtgccg attccgcctc ggatctgccg cccgcgacgg ctaccctcgc atcccgacct
      181 caccagggtg aggaggcctc ccatgcctga aatcgagcac acccctcggc cgccgcgcgg
      241 ccgtggcggc cagcccggca acctcaatgc cttcaaggac ggtcgctact ccaggcgcct
      301 cggccacgcc ttccgcatgg ccccgcccgg cgagatgcgc gatcacgtct tcgccgtgtt
      361 cgcggtcgtt gtccgcctcg agctggcccg catgggaccg cacccgaccg tcgaggagcg
      421 cgtcgccgcc atcgaacgcg ccatccgccg cctccatcgg gccggaacct cgcgcagcta
      481 ccgcggcggc cgctcgccgc aggaggtcct ccgctggttc cacgacgcca tcaacacggc
      541 gctcaccgcc acacccgagc ccatcgagcc ggctcccccg gcgccgcgcc aaacccccga
      601 aaacgaagga aacgaatgaa acgaatcacc gatcgaatct ggcgaggccg attcgtttcc
      661 ttcgtttcat tccgggctat cgaaacgaat cgaccctcgc tcgccgcagc cgcagcgagt
      721 tcgtcatcac cgacaccgag gagatcgcca tcgccagcgc cccgacgatc gggttcagga
      781 acccctgctc cccgaacacc ggctgcagca ccagcggcac cgccgtcccc agcgcctcgt
      841 gaaacacgag gtaccccaga cccgccgcca ccgggatcag caccacgttg tacccgaacg
      901 cccatgccag gttctggtgc atcgtccgca tcgtcgcccg gctcaccgcg atcgccgtcg
      961 ccaccccgcc gaggtccggg cgcatcagcg tgatcggcgc cgcctccatc gcgacgtccg
     1021 tgcccgtccc gatcgcgatc ccgacctccg ccgccgcgag ggccggcgcg tcgttgatgc
     1081 catcaccgac catcgccacg tgccgcccct gcgcctgcag ctcccgcacg acctccgact
     1141 tgccttccgg ccgcacgccg gcgatcacgc gctgtacccc cacctgcgag gccaccgctt
     1201 cggccgtcgc ctgctggtcg cccgtcagca tcaccacctc gacgcccatg gcctgcagcc
     1261 gtgcaatcgc ccccgcggag gtcgctcgta ccgggtcagc cacggcgatc aatcccgccg
     1321 ctcgcccgtc gaccgccacc agcagcgaag tctgccccac caccgcgcgt tcggtcgcct
     1381 ctcgctcgag ctccgccgcg atcacaccgc gttccgcgag cagttcgcgg ttcccgacca
     1441 gcaacgtccg ccctccgaac gtcgcctcga tgccctggcc gacgagcgct cggaacgtgt
     1501 ccggccattc gaggtcggtc ccgcgccgct ccgcctccgc caccaccgcc gaggcgagcg
     1561 gatgctccga ccctcgctcc gcggaagcga ccaggcgcac cagctcgtct tcgttgagcc
     1621 cgccggtcgc tgccagtacg ccgacgacgc tgggccgccc ctcggtcacc gtcccggtct
     1681 tgtcgaggac gaccgtgtcg actcgccgtg caacctcgag cgcctgcgcg tcccggatca
     1741 gcacgccatg ccgcgctccg aggccgctcc ccaccatgat cgcggtcggc gtcgccagcc
     1801 cgagggcgca cgggcacgcg atgatcaaca ccgtgaccgc gttgaggacc gcgagcgtaa
     1861 acgccggttc tggtccgaac gcccaccacg ccgcgaacgt cgcgagcgcg atcgcgatca
     1921 ccaccggcac gaagaccgcc gcgatgctgt cggcgacccg ctggatcggc gccttcgagc
     1981 cctgggcctc ctcgaccagc cggatgatcc gtgccagcgc cgagtccgcg cccaccgctg
     2041 tcgcgcgcac ctgcagcatg cccgtcgagt tcatcgtcgc gccgaacacc gaatccccgg
     2101 ggcctttcgg gaccggcagc ggttcgccgg tgagcatcga ctcatcaacg gcgcttccgc
     2161 ctgagacgac ctcgccatcg accggcacct gctcgctcgg ccgcaccacg acgaggtcgc
     2221 ccggctgcac cgcgctcaag gggatttcgt actcctggcc atcctcgatc acccgggcga
     2281 gacgtggccg cagcgcgatc agcgcccgca cagcctgcga agtctggccc ttcgcccggg
     2341 cttcgagcag tcgcccgagc agcaccagcg cgatgatcgc cgttgacgtg tcgaagtacg
     2401 tcgtcacctc gaactggata tggcccgcag cgccggtgtg cgcgtggccc ccaacggcgt
     2461 cgaacgcccc ggggaagaac gtggccacca ccgagtacgc gtacgcgacg gtcgtgccga
     2521 acgcgatcag cgtgttcatg tccgccgacc cgtggcggcc gacccgccag gcgccagcgt
     2581 aaaagcgcca gccggcccag aactgcaccg gtgttgcgag cgcgaagaac agcgggtgca
     2641 tcacccgagc cgggatgccg tcgaggacgg gtacgcggtg ccattgcatg ccgaccatga
     2701 ggaacgcggc cacgctgagc gcgaaccatg cccgcacgcg cagcgcgcgc acttcctcaa
     2761 gccgctcccg ctcgagcgcg tcgcgtgact cgtcctcgtc ctca
//
LOCUS       DAORPG010000217         2790 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3810_length_2790_cov_3.006216, whole genome shotgun sequence.
ACCESSION   DAORPG010000217 DAORPG010000000
VERSION     DAORPG010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3810_length_2790_cov_3.006216"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PLX85_05675"
     CDS             complement(<1..600)
                     /locus_tag="PLX85_05675"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRC62696.1"
                     /translation="MSQRTITVRVPATSANLGPGFDSLGIALDWTAHVRVTVSDSPLP
                     APEGPIERMAVTAALALYQHAAVAPPAGITASCDGDIPVGRGMGVSAFSRVAGLLAAD
                     ALISGDGEGHTREELFPLAYQLEGHGDNVAPALFGGLQVVVADDNGIVHAGLEPPADL
                     CLALLVPEFSMPTEESRAKLPQSFTRNQAVHNIGRAALLV"
     gene            742..1917
                     /locus_tag="PLX85_05680"
     CDS             742..1917
                     /locus_tag="PLX85_05680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014015981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRC62697.1"
                     /translation="MEDVVIVSGVRTAIGTLGGALSETPASDLGAAVIREAVARAGLQ
                     PEQVDQVILGCVGQVAEDGYIARHASVKGGMPIGTPAYTVNRICGSGLEAINTAARWL
                     QTGDAEVVVAGGTENMSMMPFMLRKARFGYRLGHDVIEDGTVHLVTDPFEKIHMGITA
                     ENLAEKYEVSREVQDEYSLRSQERARAAIDAGYFKEQILPLEVKKGRDTITFDTDEHP
                     RATSMELLGKLRPAFKPGGAVTAGNASGINDGAAALVVTTASKARELGLKPRFRLVAR
                     AESGVDPRTMGEGPIPAIEKALAKAGLTVSDIDTIELNEAFAAVAAACSTALGLPMEK
                     TNPNGGAIALGHPVGASGAILTVKMMHELERTGGRYGLVSLCIGGGQGIASIFERLD"
     gene            complement(1999..2277)
                     /locus_tag="PLX85_05685"
     CDS             complement(1999..2277)
                     /locus_tag="PLX85_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62698.1"
                     /translation="MPWWREDLSTFGLVIAFLAVVGGWLLSFTQFGANLIFFGMALAI
                     ASAALCPTRRRLMAAAVVVAGVWWMTGIALAAVFVGYEVVRAWRPPAD"
     gene            complement(2330..>2790)
                     /gene="tmk"
                     /locus_tag="PLX85_05690"
     CDS             complement(2330..>2790)
                     /gene="tmk"
                     /locus_tag="PLX85_05690"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014686044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRC62699.1"
                     /translation="AALFDLERAPEPLAELLVFNAARAQLVAEVIRPALERGALVLCD
                     RFTDSTVAYQHYGRGLPLETVEAVNRAATGGLAPDLTLLFDLPPEAGRARGQHGGSDY
                     LEREALAFHERVRAGYLALANAQPARWLVLDATLPIPELTRMAWERTSSD"
BASE COUNT          439 a          940 c         1033 g          378 t
ORIGIN      
        1 cacgaggagc gccgcgcggc cgatgttgtg gacggcctgg ttgcgcgtga aggattgggg
       61 cagcttggcg cgcgactcct cggtcggcat cgagaactcg gggacgagga gcgcgaggca
      121 gaggtccgcg ggcggctcga ggccggcgtg gacgatccca ttgtcgtcgg cgacgacgac
      181 ctggagtccg ccgaagagcg cgggggccac gttgtcgccg tgcccttcga gctggtaggc
      241 gagcggaaac agctcctcac gggtgtggcc ctcgccgtcc ccgctgatga gggcgtcggc
      301 ggccaggagg ccggcgacgc gcgagaaggc agacacgccc atgccccggc cgaccgggat
      361 gtcgccgtcg caactcgcgg tgatgcccgc gggcggcgcg acggcggcgt gctggtagag
      421 cgcgagggcc gcggtgacgg ccatgcgttc gatgggaccc tcgggcgcgg gcagcgggga
      481 atcgctcact gtgacccgca cgtgggcggt ccagtcgagg gcgatcccca ggctgtcgaa
      541 gccggggccg agattggcgg aggttgctgg gactcgtacg gttatggttc gctgtgacat
      601 cgccgcccag tgtatgggtg gctgaacacc cgggcaattg aaggcctcgg gcgccccgcc
      661 gtgccgtttt gcccgcgata tactttgttg gtaggctctc tcggcatcgt ctctgaaaca
      721 ctccagaaag gcgggagtcg catggaagac gtcgtcatcg tcagcggtgt ccgcaccgcg
      781 atcgggacgc tgggcggcgc gctcagcgag acgcccgcgt ccgacctcgg cgccgccgtc
      841 atccgcgagg cggtggcccg cgcgggcctc cagcccgaac aggtcgacca agtcatcctc
      901 ggctgcgtgg gccaggtggc cgaggacggg tacatcgccc gtcacgcctc cgtgaagggc
      961 ggcatgccga tcgggacgcc cgcctacacc gtgaaccgca tctgcggctc gggcctcgag
     1021 gccatcaaca cggctgcgcg ctggctccag accggcgacg ccgaggtggt cgtcgccggt
     1081 ggcaccgaga acatgtcgat gatgccgttc atgctccgca aggcgcgctt cggctaccgc
     1141 ctgggccatg acgtgatcga ggacggcacc gtccacctcg tgaccgaccc gttcgagaag
     1201 atccacatgg gcatcaccgc cgagaacctc gccgagaagt acgaggtctc ccgcgaggtg
     1261 caggacgagt actcgttgcg ctcgcaggag cgcgcccgcg ccgccatcga cgccggctac
     1321 ttcaaggagc agatcctccc gctcgaggtg aagaagggcc gtgacacgat caccttcgac
     1381 actgacgagc acccccgcgc gacctcgatg gagctcctgg gcaagctgcg gccggcgttc
     1441 aagccgggcg gcgcggtgac ggccggcaac gcctccggca tcaacgacgg cgccgcggcg
     1501 ctcgtcgtca cgaccgcctc gaaggcgcgc gagctggggc tgaagccgcg cttccgcctc
     1561 gtcgcgcgtg cggagtcggg cgtcgacccg cgtacgatgg gcgagggccc gatccccgcg
     1621 atcgagaagg cgttggcgaa ggccggcctg accgtcagcg acatcgacac gatcgagctg
     1681 aacgaggcgt tcgcggcggt cgccgcggcc tgctcgacgg cgctcggcct gccgatggag
     1741 aagacgaacc cgaacggcgg cgcgatcgcc ctcggccacc cggtcggggc cagcggcgcg
     1801 atcctcaccg tgaagatgat gcacgagctg gagcgcaccg gcggccgcta cggcctggtg
     1861 tcgctctgca ttggcggcgg ccagggcatc gcgagcatct tcgagcggct cgactaggcc
     1921 ctcggcactc ccgaaacgag cacagacggc ggccgtgagg ccgccgtctt gttgcgtgac
     1981 gcgtgactca ggacttccct agtcagccgg cggcctccat gcgcggacga cctcgtagcc
     2041 aacgaacacg gccgccagag cgatccccgt catccaccag acgcccgcca ccacgacggc
     2101 tgcggccatc agccggcgcc gcgtcgggca gagggccgcc gaggcgatgg cgagcgccat
     2161 gccgaagaag atgagattcg cgccgaactg ggtgaacgag agcagccagc cgccgaccac
     2221 cgcgaggaac gcgatgacga ggccgaacgt actgaggtcc tcgcgccacc agggcatgtg
     2281 accgcctcga acatcgtgac tgttagcggc tcaattcgcg ttgttctcgt cagtccgacg
     2341 aggtccgctc ccacgccatc cgcgtgagct ccgggatcgg cagcgtcgcg tccagcacca
     2401 gccagcgcgc gggctgcgcg ttcgccagcg cgaggtaccc ggcgcgcacg cgctcgtgga
     2461 aggcgagtgc ttcgcgctcg aggtagtccg agcccccgtg ctggccgcgg gcgcggccag
     2521 cctcgggggg caggtcgaag aggagtgtga ggtcgggggc gaggccgccg gtggcggcgc
     2581 ggttcaccgc ctcgacggtt tcgaggggga ggccccggcc gtagtgctga taggcaacgg
     2641 tcgagtcggt gaagcggtcg cagaggacga gggcgccgcg ctcgagcgcg gggcggatca
     2701 cctcggcgac gagctgggcg cgcgcggcgt tgaagacgag cagctcggcc agcggctccg
     2761 gggcgcgttc gaggtcaaac agcgccgcgc
//
LOCUS       DAORPG010000218         2784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3826_length_2784_cov_3.737266, whole genome shotgun sequence.
ACCESSION   DAORPG010000218 DAORPG010000000
VERSION     DAORPG010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3826_length_2784_cov_3.737266"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..644
                     /locus_tag="PLX85_05695"
     CDS             141..644
                     /locus_tag="PLX85_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HRC62700.1"
                     /translation="MVTVDDVKRLREETGAGVMDAKRALDEAGGDFNKAKELLRERGI
                     AAAAKRAERSTGQGVVEAYIHGGGRIGALVEVNCETDFVARTDAFRTLARDIAMQVAA
                     MNPAALTPEDVPGDLPGEAKDHALLTQTFIKDGSRSIQDLVQDVIAQTGENIRVARFS
                     RFELGGA"
     gene            822..1379
                     /gene="frr"
                     /locus_tag="PLX85_05700"
     CDS             822..1379
                     /gene="frr"
                     /locus_tag="PLX85_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRC62701.1"
                     /translation="MTDDILLDADDRMDKAVLAFQHNLDTIRTGRANTALVEHLQVTH
                     YGQTLPLNQLATLAAPESTLITVQPWDRGAMDSIMKAIQTSDLGINPSNDGRLIRLPI
                     PPLTEQRRRDLVKQVHTRAEEARVAIRNIRRHAIDEVKKAVKAGDVPEDDGRHAEDEV
                     EKLTHAQTDRIEQLSKTKERELLEV"
     gene            1407..2162
                     /gene="uppS"
                     /locus_tag="PLX85_05705"
     CDS             1407..2162
                     /gene="uppS"
                     /locus_tag="PLX85_05705"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HRC62702.1"
                     /translation="MVVLPQRAELPLPHIHSLPRHVAIIMDGNGRWARARGLSRNDGH
                     RAGAEALRSVIERFAEHGVEAVTLFAFSTENWGRPRSEVDGIMRLAARVIDRELNTFH
                     ESGIRLQHLGDLAALPKGLQSRVAKAVRRTQQNTGMTVNLAFNYGGRADIVEAVRHLV
                     AAGVRPEDVTEAAISSHLTTAALPDPDLLIRTGGERRISNFLVWQAAYSEYYFTPVQW
                     PDFGAAEVDEALLEFSRRRRRFGLVPEDVGTDA"
     gene            2166..>2784
                     /locus_tag="PLX85_05710"
     CDS             2166..>2784
                     /locus_tag="PLX85_05710"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRC62703.1"
                     /translation="MLKQRLAVAAVGLPLLALLVASPERVFGLGVALILGAATYEVVH
                     AAIPATTRTSALAAGVLAALLATSFRTLEEIPVWSLPAMVAVTLLLLALLLWPGPGQA
                     PAGAWWLGAVLYLGVLGAHWLLLRNEDHGQAWVVVAFAVTFATDTGAYAVGRLVGRHL
                     FWPAVSPKKTWEGAGGAVVFGGAMAAGLPFALGIQPGWPWVAAIAS"
BASE COUNT          416 a          967 c          995 g          406 t
ORIGIN      
        1 cggccgaggc tcccgctccg gacggcgccg cgagctagtc catcgcacgt actcgtggcc
       61 gcgctcacgt attgggcgcg gccactgctt gaacccctat gccgcctcga agcgacggcg
      121 gctcagctga ggagcgatca gtggtcacag tcgacgatgt gaagcgcctg cgcgaggaaa
      181 ccggcgcggg cgtcatggac gccaagcgcg ccctcgatga ggcgggcggc gacttcaaca
      241 aggcgaagga gctgctgcgc gagcgtggca tcgccgccgc ggccaagcgc gcggagcgct
      301 cgacgggcca gggcgtcgtc gaggcgtaca tccacggcgg tggccgcatc ggcgcgctcg
      361 tcgaggtgaa ctgcgagacc gacttcgtgg cgcgcacgga cgccttccgg acattggcgc
      421 gcgacatcgc gatgcaggtg gcggcgatga acccggcggc gctgacgccg gaggacgtgc
      481 cgggcgacct gccgggcgag gcgaaggacc acgcgctgct cacgcagacc ttcatcaagg
      541 acggcagccg ctcgatccag gacctggtgc aggacgtgat cgcgcagacg ggcgagaaca
      601 tccgcgtcgc gcgtttctcg cgcttcgaac tcgggggcgc ctgacccgcg ctcctcgaac
      661 gcggggacgc tcgatgtgag ggcgtccctg cgttatcgtg acagacaggc tcccacctcg
      721 cgcatacccg tgctttcggc cgggcatacg ctggaacagg tcagtgcacg ccgatcctcg
      781 gacgcggcga gcgggcctgc gagcaggaca ggaggacacc ggtgaccgat gacatcctcc
      841 tcgatgccga cgaccgcatg gacaaggccg tgctggcgtt ccagcacaat ctcgacacga
      901 tccgcacggg ccgcgcgaac acggcgctgg tcgagcacct gcaggtgacg cattacggac
      961 agacgttgcc gctgaaccag ctggcgacgc tcgcggcgcc cgagtccacg ttgatcaccg
     1021 tccagccgtg ggaccgcggc gcgatggact cgatcatgaa ggcgatccag acgagcgacc
     1081 tcggcatcaa cccctcgaac gacgggcggt tgatccggct gccgattccg ccgctcaccg
     1141 agcagcgccg gcgagacctc gtgaagcagg tgcacacgcg ggccgaggag gcgcgcgtcg
     1201 cgatccgcaa catccggcgg cacgccatcg acgaggtgaa gaaggccgtg aaggccggcg
     1261 acgtccccga ggacgatggc cgccacgccg aggacgaggt cgagaagctg acccacgcgc
     1321 agaccgaccg catcgagcag ctgagcaaga ccaaggagcg cgagcttctt gaggtctgag
     1381 cgccgcccct ccttcgagcc cgcccgatgg tagtgctgcc gcagcgcgcc gagctgccgc
     1441 tgccccacat ccattcattg ccgaggcacg tcgcgatcat catggacggc aacgggcgct
     1501 gggctcgcgc ccgtgggttg tcgcgcaacg atggacatcg cgcgggcgcc gaggcgctgc
     1561 gcagcgtcat cgagcgcttc gccgagcacg gcgttgaggc ggtgaccctg ttcgcgtttt
     1621 cgacggaaaa ctggggccgt cctcgctctg aggtcgatgg gatcatgcgc ctcgcggcgc
     1681 gcgtcatcga tcgcgagctg aacacattcc atgagtcggg catccggctg cagcacctcg
     1741 gcgaccttgc cgcgctgccc aaggggctgc agtcccgcgt cgcgaaggcg gtgcggcgga
     1801 cgcagcagaa cacgggcatg accgtcaacc tggccttcaa ctatggcggc cgcgcggaca
     1861 tcgtcgaggc ggtgcgccac ctcgtcgctg ccggggtgcg ccccgaggac gtgactgagg
     1921 cggcgatctc ctcgcacctg acgacggcgg cgctgccgga tcccgacctg ctcatccgca
     1981 ccggcggcga gcggcgcatc tcgaacttcc tcgtctggca ggccgcctac agtgagtact
     2041 atttcacgcc cgtgcagtgg cccgacttcg gcgcggccga ggtggacgag gcgctgctgg
     2101 agttcagccg ccgccggcga cggttcggcc tcgtcccgga ggacgtcggg acggacgcct
     2161 gagcggtgct gaagcagcgg ctcgcggtcg ccgccgtcgg gctgccgctg ctcgccctcc
     2221 ttgtcgcctc acctgaacgg gtcttcggcc tcggcgtggc gctcatcctc ggcgcggcca
     2281 cgtacgaggt cgtgcacgcc gcgatcccgg cgaccactcg cacttcggcg ctcgccgcgg
     2341 gcgtcctcgc cgcgctcctc gcgacctctt tccgcacgct cgaggagatc ccggtctggt
     2401 cgctgcccgc aatggtggcg gtcacgctgt tgctgctcgc gctgctgctc tggcccggcc
     2461 ccgggcaggc gccggcgggc gcctggtggc tcggcgccgt gctgtacctc ggtgtgctgg
     2521 gggcgcactg gctgctgctg cgcaacgagg atcacgggca ggcctgggtc gtagtcgcct
     2581 tcgcggtcac gttcgctaca gacaccgggg cgtatgcggt gggacgcctc gtgggccggc
     2641 acctgttctg gccggccgta tcgccgaaga agacgtggga gggcgcgggc ggagccgtcg
     2701 tgttcggcgg ggcgatggcg gcgggactgc ccttcgccct cggcatccag ccgggctggc
     2761 cctgggtcgc cgccatcgcc tcga
//
LOCUS       DAORPG010000219         2778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3843_length_2778_cov_3.877708, whole genome shotgun sequence.
ACCESSION   DAORPG010000219 DAORPG010000000
VERSION     DAORPG010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3843_length_2778_cov_3.877708"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /gene="rplX"
                     /locus_tag="PLX85_05715"
     CDS             <1..259
                     /gene="rplX"
                     /locus_tag="PLX85_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRC62704.1"
                     /translation="KRGEVRRVLPAEQRVVVAGLNMVKRHRRQRNPQEASAIITLEAP
                     LHVSNVKVVCRSCNEAVRVGFKFLDDGRKVRVCKNCNEAID"
     gene            259..819
                     /gene="rplE"
                     /locus_tag="PLX85_05720"
     CDS             259..819
                     /gene="rplE"
                     /locus_tag="PLX85_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018784556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRC62705.1"
                     /translation="MPETTTLPALKQRYRDEVVAVLTREFGYTNPMQVPTLQKVSINI
                     GLGEALTNGRAIESAQQDLATITGQRPVVNRAKKAISNFKLREGNPIGVSLTLRGDRM
                     WEFLDRLMNVALPRVRDFRGVNANAFDGRGNYALGLTEQIIFPEITFDQVERVRGMQI
                     NVITTARTDEEGKRLLELLGMPFTKS"
     gene            871..1074
                     /locus_tag="PLX85_05725"
     CDS             871..1074
                     /locus_tag="PLX85_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRC62706.1"
                     /translation="MAKMSQIVKSNRKPKFANRHVNRCPVVWANRPCGRPRGYMRQFG
                     MCRICFRTLANQGKLPGVKKSSW"
     gene            1114..1512
                     /gene="rpsH"
                     /locus_tag="PLX85_05730"
     CDS             1114..1512
                     /gene="rpsH"
                     /locus_tag="PLX85_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRC62707.1"
                     /translation="MSMSDPLADMCTRIRNAVAARHETVRVPHSKVKRAVADVLQREG
                     YIAGYTEEAEGARQYLRLRLNYRPDRRSTLSGIRRVSRPGLRVYVNKGEIPRVYGGLG
                     VAIMSTSHGVMSGQEAWRAKVGGEVLCYVW"
     gene            1522..2058
                     /gene="rplF"
                     /locus_tag="PLX85_05735"
     CDS             1522..2058
                     /gene="rplF"
                     /locus_tag="PLX85_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRC62708.1"
                     /translation="MSRVGRLPIPIPAGVEVTLEGQRARVKGPRGQLEHDVHPSIAVS
                     LDEGTVVVTRPSNAPTHRALHGLTRALLANMVTGVSDGFRKSLEIQGTGYRAELRGSN
                     LVLNVGYSHPVELQPLEGAAFEVETPTRVHVVGIDKQVVGQQAALIRKQRPPEPYRGK
                     GIRYAGEVVRRKAGKSGR"
     gene            2067..2429
                     /gene="rplR"
                     /locus_tag="PLX85_05740"
     CDS             2067..2429
                     /gene="rplR"
                     /locus_tag="PLX85_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRC62709.1"
                     /translation="MSATDTTRVARIRRHVRIRKKVAGTPEQPRLAVFRSGQHIYAQV
                     IDDRAGRTLAQASDLEAALRDGGGTKTDRARQVGELVARRAKEAGLETVVFDRGGFAY
                     AGRVKALADAARAEGLVF"
     gene            2429..>2778
                     /locus_tag="PLX85_05745"
     CDS             2429..>2778
                     /locus_tag="PLX85_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRC62710.1"
                     /translation="MVQRERGERGPRRERRRDDDEQSDYIEKVVFINRVAKVVKGGRR
                     FSFTAIVVVGDGHGRVGFGMGRANEVPEAIRKGGVIARRSMIQVPMRHGTISHDTMSQ
                     FKASKVIMRPASAGT"
BASE COUNT          486 a         1041 c          850 g          401 t
ORIGIN      
        1 gaagcgcggc gaggtacgtc gcgtcctccc cgccgagcag cgcgtcgtcg tcgccggcct
       61 caacatggtc aagcgccacc gccgccagcg gaacccgcag gaagcctccg ccatcatcac
      121 gctcgaggcg cccctgcacg tctccaacgt caaggtcgtc tgccgctcct gcaatgaggc
      181 cgtccgcgtc gggttcaagt tcctcgacga cggccgcaag gtgcgcgtct gcaagaactg
      241 caacgaggcc atcgactaat gcccgagacc actaccctcc ccgcgctcaa gcagcgctac
      301 cgcgacgagg tcgtcgccgt cctcactcgt gagttcggct acacgaaccc gatgcaggtg
      361 ccgaccctcc agaaggtctc gatcaacatc ggcctcggtg aggccctcac gaacggtcgc
      421 gccatcgagt cggcccagca ggacctggcc accatcaccg gccagcgccc cgtcgtgaac
      481 cgcgccaaga aggccatctc caacttcaag ctccgcgagg gcaaccccat cggcgtctcg
      541 ctcaccctgc gcggcgaccg catgtgggag ttcctcgacc gcctcatgaa cgtcgcgctc
      601 ccccgtgtcc gcgacttccg aggagtcaat gccaacgcct tcgatggccg cggcaactac
      661 gcactcggcc tcaccgagca gatcatcttc cccgagatca cgttcgacca ggtcgaacgc
      721 gtccgtggga tgcagatcaa cgtcatcacc accgcacgca ccgacgaaga aggcaagcgg
      781 ctgctcgagc tcctcggcat gccgttcacc aagagctagt cgcggcacca agaagactcg
      841 ccacgagcga ggagtaaggg gatacccacg atggcgaaga tgtcgcagat cgtgaagtcc
      901 aaccgcaaac caaagttcgc caaccggcac gtcaaccgct gcccggtcgt ctgggcgaac
      961 cggccctgcg gacgtccgcg cggctacatg cgccagttcg gcatgtgccg catttgcttc
     1021 cgtacgctcg ccaaccaggg caagctgccc ggcgtcaaga aatcctcctg gtagtcggct
     1081 gaccgaggcc agagagaagg caggacgaga ccgatgagca tgtctgaccc gctcgctgac
     1141 atgtgcacac gcatccgcaa cgccgtggcc gcgcgccacg agaccgtgcg cgtccctcat
     1201 tccaaggtga agcgcgccgt cgcggacgtg ctccagcgcg agggctacat cgccggctac
     1261 actgaggagg ccgaaggtgc tcgccagtac ctccgcctcc gcctcaacta ccgccccgac
     1321 cgccgctcca cgctctccgg catccgccgt gtctcgcgtc ccgggctgcg cgtctacgtc
     1381 aacaagggag agattccgag ggtctacggt ggcctcggag tcgccatcat gagcacgtcg
     1441 cacggcgtca tgtccggcca ggaagcctgg cgggccaagg tcggcggcga agtgctctgc
     1501 tacgtctggt aagggaccgc catgtcgcgc gtaggccggc tgcctattcc catccccgcg
     1561 ggcgtcgaag tcacgctcga gggccagcgc gcccgcgtta aggggccgcg tggccagctc
     1621 gaacacgacg tccacccctc catcgccgtc tccctcgatg agggcacggt cgtggtcacc
     1681 cgcccctcga acgcgcccac ccaccgcgcg ctccacgggc ttacccgcgc ccttctcgcc
     1741 aacatggtga ccggcgtctc ggacggcttc cgtaagtccc tcgagatcca gggcaccggt
     1801 taccgcgccg agctgcgtgg ctccaacctc gtgctcaacg tcggctactc ccaccccgtc
     1861 gagctgcagc ccctcgaggg cgcggccttc gaggtggaga cgccgacccg tgtacacgtt
     1921 gtcggcatcg acaagcaggt cgtcggccag caggccgccc tcatccgcaa gcagcggcct
     1981 cccgagccct accggggcaa gggcatccgc tacgccggtg aggtcgtccg ccgcaaggcc
     2041 gggaagtctg gacggtagga gaggcgatgt cagctaccga caccacccgc gtcgcccgca
     2101 tccgccggca cgtgcgcatc cgcaagaagg tcgccggcac gcccgagcag ccacggctgg
     2161 ccgtgttccg ctccggccag cacatctacg cgcaggtcat cgatgaccgc gccggccgca
     2221 ccctcgcaca ggcctcggat ctcgaggcag ccctgcgcga cggcggcggc accaagaccg
     2281 atcgcgctcg ccaggtcggc gaactcgtcg cgcgccgcgc gaaggaagcc ggcctcgaaa
     2341 ctgtcgtctt cgaccgcggc ggcttcgcct acgccggtcg cgtcaaggcc ctcgccgatg
     2401 cggcccgggc cgaagggctg gtgttctgat ggtgcaacgt gaacgaggcg agcgcggtcc
     2461 ccgccgcgaa cgccggcgcg acgatgatga gcagagcgac tacatcgaga aggtcgtctt
     2521 catcaaccgc gtcgccaagg tggtcaaggg cggtcgccgc ttctccttca ccgccatcgt
     2581 cgtcgtcggc gacggccacg gccgcgtcgg cttcggcatg ggccgcgcca acgaggtccc
     2641 cgaggccatc cgcaagggcg gcgtcatcgc gcgccgctcg atgatccagg tcccgatgcg
     2701 ccacggcacc atctcccacg acaccatgtc gcagttcaag gcctcgaagg tcatcatgcg
     2761 gcccgcgtcc gccggtac
//
LOCUS       DAORPG010000220         2765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3869_length_2765_cov_3.135424, whole genome shotgun sequence.
ACCESSION   DAORPG010000220 DAORPG010000000
VERSION     DAORPG010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3869_length_2765_cov_3.135424"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..381
                     /locus_tag="PLX85_05750"
     CDS             130..381
                     /locus_tag="PLX85_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRC62711.1"
                     /translation="MPIRGYILVETEVGRTQAVGRAMRDLAPTLARVLAVDTVTGPFD
                     VIVQLEADDLDMLGKCITEDIQRVPGVQRTTTCLSVQLG"
     gene            424..1731
                     /locus_tag="PLX85_05755"
     CDS             424..1731
                     /locus_tag="PLX85_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRC62712.1"
                     /translation="MTTIAHHGPPGPSGMISVEEARARILEHFAPLPAEQTPLTEVLG
                     QVLAEDVRASFSIPPMANTSMDGYAVRAADTEGAAPSTPRELRVTGYLAAGSLFNGAV
                     GPGEAVRIMTGAPMPDGADAVVPFEETDEQEHGRWSDAPRERVRVDVAARLGANIRRA
                     GEDVRAGEVVLAAGTVLGPAQIGVLASLGLDHAPVHRRPRVAVLSTGDELLRPGEPLR
                     PGKIYDSNAFGIAAAVRDCGGVPTILDVARDSVEALTARIHEALETADLLITSAGVSR
                     GDFDVVKEVLAREGEVGFWTVRMRPGKPLAFGTFRAAGDRVVPHLGLPGNPVSALMTF
                     ELFGRPALFTLLGKPEAWVRPTVRAVTHERIVNPDPRRFYARAIVRSDGDGYVVNLTG
                     PQGSGVLTSMALANGYAIVPEDVATIEAGEECDVILLGAPLPL"
     gene            1728..2237
                     /locus_tag="PLX85_05760"
     CDS             1728..2237
                     /locus_tag="PLX85_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62713.1"
                     /translation="MTHPALFCVVGPPASGKSLLITALTEALRRRGRRVASVVLRDDS
                     TAVFVLSNGGRVTVSRHFDASELVEFVGSIEPNLDVLLAEGFRAAGCATIRISSDDDG
                     TWRVDDADLVATVPTVALLGDLGVFATGEGDRLAALIEERLGGEKQESPSADGGQGFL
                     GRWRRRGRR"
     gene            complement(2242..>2765)
                     /locus_tag="PLX85_05765"
     CDS             complement(2242..>2765)
                     /locus_tag="PLX85_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62714.1"
                     /translation="VDALFNAANTNADLKAVKWYGSDSVALSADLLSDKTAAAFAQAA
                     YYPNPILGLSEADKSRWAPVQQQLAGTLGRQPDAFALAAYDALMVAHEALDKAGGDKA
                     EVADLKKQFVTLANGHQGLTGPTKLNAAGDRDIAIYDFWAVCKQNNDFFWYRAASYVA
                     GESNPISRPEKCQ"
BASE COUNT          406 a          879 c         1046 g          434 t
ORIGIN      
        1 gccgagcggc gtcggtggga gtgcccgcgc tcgtgagcgg cgcggaggcg tgaacggccg
       61 cgtgcgtggc gtcgcgctct cgtacaatca cgggaggaga ctgggcgagc accactgaaa
      121 ggtcccgcga tgccgattcg aggctacatc ctggtcgaga ccgaagtcgg acgaacacag
      181 gccgtgggac gcgcgatgcg cgacctggcg ccgacgctgg cacgcgtgct ggcggtggac
      241 accgttacgg ggccgttcga cgtgatcgtg cagctggagg ccgatgacct cgacatgctg
      301 ggcaagtgca tcacggagga catccagcgg gtgccgggcg tgcagcgcac gaccacctgc
      361 ctgtcggtgc agctcgggta gtccgccggc aatgacacgg gcggccctcg agggcggcag
      421 agcgtgacga ccatcgccca ccacgggccg ccggggccgt cgggcatgat ctcggtggag
      481 gaagcgcgcg cgcgcatcct cgagcacttc gcaccactgc cagcagagca gacgccgctg
      541 accgaggtgc tggggcaggt gctagccgag gacgtgcggg ccagcttcag catcccgccg
      601 atggccaaca cgagcatgga cgggtacgcc gtacgagcgg cagacaccga gggggcagca
      661 ccctccacgc cgcgggagct gcgcgtcacg ggctatctcg ccgcgggttc actcttcaac
      721 ggcgccgtcg ggcctggcga ggccgtgcgc atcatgacgg gcgcgccgat gccggacggt
      781 gcggacgcgg tcgtcccctt cgaggagact gacgaacagg agcacgggcg ctggagcgac
      841 gctccccgcg agcgcgttcg agtcgacgtc gcggcgcgcc tcggagcgaa catccggcgg
      901 gccggcgagg acgtgcgggc gggtgaagtc gtgctcgcgg cgggcaccgt gctcgggccg
      961 gcacaaatcg gtgtgcttgc ctcgctgggg ctcgaccacg caccggtaca ccgacgtccg
     1021 cgggtcgcgg tgctgtcgac gggcgacgag ctgctgcggc cgggcgagcc actgcggccg
     1081 ggcaagatct acgactcaaa cgccttcggg attgcggcgg cggtgcgaga ctgcggcggt
     1141 gtcccgacca tcctcgatgt cgcgcgcgac agcgtagagg cgttgacggc gcgcatccac
     1201 gaggccctcg agacagcgga cctgctcatt acctccgcgg gcgtgtcgcg gggcgatttc
     1261 gacgtggtga aggaagtgct ggcacgcgag ggcgaggtcg gtttctggac ggtgcgcatg
     1321 cggccgggga agccgctggc gtttggcacc ttccgggcgg ctggtgaccg ggtcgtcccc
     1381 cacctcggac tgccgggcaa cccagtgtcc gcgctcatga cgttcgagct gttcggccgg
     1441 ccggcgctgt tcaccctgct gggcaagccg gaggcgtggg tacgcccgac cgtgcgcgca
     1501 gtcacgcatg agcgcatcgt gaatcccgac ccgcggcggt tctacgcgcg cgccatcgtc
     1561 aggtcggatg gcgatggtta tgtcgtgaac ctaacggggc cacaggggtc cggcgtgctg
     1621 acctcgatgg cgctcgccaa tgggtacgcg atcgtgccgg aggacgtggc gacgatcgag
     1681 gcgggcgagg agtgcgacgt gatcctcctc ggagcgcccc tgccgctgtg acccaccccg
     1741 ccctgttctg cgtggtgggt ccgccggcgt ccggtaaatc actgctcatc acggcgctga
     1801 cggaggcgct gcgacgacgg gggcgccgcg tggcctcggt cgtgctgcgc gacgattcga
     1861 cggccgtatt cgtgctgtcg aacggcggac gtgtcacggt gtcgcgtcac ttcgacgcca
     1921 gcgagctggt tgagttcgtg ggatcgatcg agcccaacct cgatgtgctg ctcgcggaag
     1981 gattccgcgc agcgggatgc gcgaccatcc ggatctcctc ggacgacgat ggcacgtggc
     2041 gtgtggacga tgccgacctc gtagcgacgg tgccgacggt ggcgttactg ggcgacctcg
     2101 gcgtgtttgc cactggcgag ggtgaccgac tcgcggcgct gatcgaagag cggttggggg
     2161 gcgagaagca ggaatccccg tccgcggacg gcggacaggg attcctcggt cgatggcgac
     2221 ggcgcggacg ccgctagggc gctactggca cttctccggg cgggagatcg ggttgctctc
     2281 gcccgccacg tacgacgcgg cgcgatacca gaagaagtcg ttgttctgct tgcagacggc
     2341 ccagaaatcg tagatggcga tgtcgcggtc gcccgcggcg ttcagcttcg tggggccggt
     2401 caggccctgg tggccatttg ccagggtgac gaactgcttc ttgagatcgg cgacctcggc
     2461 cttgtccccg ccggccttgt cgagcgcctc gtgggcgacc atgagggcgt cataggcggc
     2521 gagcgcgaag gcgtctggct ggcgcccaag cgtgccagcg agctgctgct gcaccggcgc
     2581 ccaacggctc ttgtcggctt cgctcaggcc gaggatgggg ttggggtagt aggcagcctg
     2641 cgcaaacgcg gcggcggtct tgtcactgag caggtccgcg ctcaaggcga cgctgtcgct
     2701 gccgtaccac ttcaccgcct tgaggtcggc gttcgtgttg gcagcgttga agagggcgtc
     2761 cacct
//
LOCUS       DAORPG010000221         2762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3875_length_2762_cov_3.456225, whole genome shotgun sequence.
ACCESSION   DAORPG010000221 DAORPG010000000
VERSION     DAORPG010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3875_length_2762_cov_3.456225"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PLX85_05770"
     CDS             complement(<1..330)
                     /locus_tag="PLX85_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62715.1"
                     /translation="MPTSIEAGLCGHCRWAQRIQSARGSEFIRCRRSDDDSAFPRYPR
                     LPMHSCPGFQTNRPADAPPLPRRAPGARPPEVLVTNPNEPLFERLGGREGVERIVDRF
                     YDLVEADP"
     gene            371..583
                     /locus_tag="PLX85_05775"
     CDS             371..583
                     /locus_tag="PLX85_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62716.1"
                     /translation="MRATIEAVQPYDLHGVRYYRVIYRGEGEDGLREARLSHDMVYAD
                     PQEGDLVEVRAILGIVDRIAKVEAGG"
     gene            629..1498
                     /gene="purU"
                     /locus_tag="PLX85_05780"
     CDS             629..1498
                     /gene="purU"
                     /locus_tag="PLX85_05780"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HRC62717.1"
                     /translation="MVEQAPGTARLLVQCPDKPGIVAAVSSFLYEHGANIVQADQYSS
                     EDGRGQFFLRLEYELAGLDAPAQRIASEFGERIAPGFEMQWEVAYSADHRRMAILVSK
                     ADHCLLELLWRWRRGELAAEISMVISNHDDLRREVEAFDIPYHHLPVTAATKPEQERA
                     IRELLRGQADLVVLARYMQVLSPAFIEEYPHAVINIHHSFLPAFAGADPYGQAFDRGV
                     KIIGATAHYATEQLDEGPIIEQDVVRVSHRDTRADLVRHGREVERSVLARAVQWHLED
                     RVLVHGNKTVVFR"
     gene            complement(1495..1968)
                     /locus_tag="PLX85_05785"
     CDS             complement(1495..1968)
                     /locus_tag="PLX85_05785"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRC62718.1"
                     /translation="MFTAENTVTIDRPVGEVFAYAMDPLRIHEWRPNVLEIIDYEPPL
                     RAGTEYQIAENMMGRKVFGQRVTEYQQDRLVVIETTSGSVRPVQRFSVEPLSEGSTRY
                     TARLDITTHGFMRLMEPMFRGSVRKRMVEYGNNLKRNIEALPRVEADARPGGTQP"
     gene            2110..>2762
                     /locus_tag="PLX85_05790"
     CDS             2110..>2762
                     /locus_tag="PLX85_05790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC62719.1"
                     /translation="MVLWIAMAVAMAGIGMVSPLLPVYVREELHGPELAVALSFSGLA
                     VTQLAMSPLVGRLGDRHGPKILIVVGFLIYGAAGLGYLFSHHWALVIAFRLLSGVGAA
                     AIFPMALSYVGRLAPPGREGTYMGMYAVAETAGFGLGPLLGGAVRDLVSSQAAFLTMS
                     VALCGTGLLALIALPAAAYAGQPFFSSALRALRARQVNMDVPISLGVLLALGMSVVET
                     "
BASE COUNT          397 a          851 c         1043 g          471 t
ORIGIN      
        1 ggggtcggcc tccacgagat cgtagaaacg gtcgacgatg cgctccacgc cctcacgacc
       61 gccgaggcgc tcgaacagcg gctcattcgg gtttgtcacg aggacctccg gtggtcgcgc
      121 gccgggggcg cgcctgggca ggggcggcgc gtccgcgggg cggttggtct ggaagccggg
      181 gcacgagtgc atcggcagcc gtgggtacct tgggaatgcc gaatcgtcgt cggagcggcg
      241 gcagcggatg aactcggagc cgcgggcgct ctggatgcgc tgcgcccagc ggcagtgccc
      301 gcagagcccg gcctcgatgg aagtcggcat cggactcagt ctaccctcga agcgcgggcg
      361 ggaggcgacg atgcgagcaa cgatcgaggc agtacagccg tacgacctgc acggggtgcg
      421 gtattaccgc gtgatctacc gcggcgaagg cgaggacggc ctgcgcgagg cgcggctgtc
      481 gcacgacatg gtgtacgcag acccgcagga gggcgatctg gttgaggtgc gcgcgatcct
      541 cgggatcgtc gaccggatcg cgaaggttga ggcgggcggg tagtgccgcg catggccgct
      601 gaccgagggc gcacctatca tccgggccat ggttgagcag gcgccgggga cggcgcggtt
      661 actcgtgcag tgcccggaca agcccggaat cgtggcggcg gtctcgtcgt tcctgtacga
      721 gcacggggcg aacatcgtcc aggccgacca gtactcctcg gaggacgggc gagggcagtt
      781 cttcctgcgc ctggaatacg agctggcggg gctggacgcg ccggcgcagc ggatcgcctc
      841 ggagttcggg gagcggatcg ctccggggtt cgagatgcag tgggaggtcg cgtacagcgc
      901 cgaccatcgt cggatggcga tcctcgtgtc gaaggcggac cactgtctcc tcgaactgct
      961 gtggcggtgg cggcggggcg agctggcagc cgagatctcg atggtgatct cgaaccatga
     1021 cgacctgcgc cgagaggtgg aggcgttcga catcccgtac caccacctgc cggtgaccgc
     1081 ggccacgaag ccggagcagg agcgagcgat ccgcgagctg ctgcgcgggc aggcagacct
     1141 cgtcgtgctg gcgcggtaca tgcaggtgct gtcgccggcg ttcatcgagg agtacccgca
     1201 cgcggtgatc aacatccatc actcgttcct gccggcgttc gcgggcgcgg acccgtacgg
     1261 gcaggcattc gaccgcgggg tcaagatcat cggtgcgacg gcccactacg cgaccgagca
     1321 gctcgacgag gggccgatca tcgagcagga cgtggtgcgg gtgtcgcatc gtgacacgcg
     1381 ggccgacctc gtgcggcacg ggcgtgaggt cgaacgctcc gtgctggcgc gcgcggtgca
     1441 gtggcacctc gaggaccggg tgctcgtgca cggcaacaag acggtggtct tccgctaggg
     1501 ttgcgtgcct cccggtctgg cgtcagcctc cacgcggggt agcgcctcga tgttgcgctt
     1561 cagattgttg ccgtactcga ccatgcgctt gcggacactg cctcggaaca tcggctccat
     1621 caggcgcatg aagccatgcg tggtgatgtc gaggcgtgcg gtgtagcgcg tggagccctc
     1681 ggagagcggc tcgacggaga accgctggac cgggcggacc gaacctgacg ttgtctcgat
     1741 aacgacgagt cggtcctgct ggtactcggt gacccgctgg ccgaagacct tgcggcccat
     1801 catgttctcg gcgatctggt actccgtccc ggcgcgcagc ggcggctcgt agtcgatgat
     1861 ctcgaggacg ttgggccgcc actcatggat tctcagcggg tccatcgcat aggcgaagac
     1921 ctcgccgacc gggcggtcga tcgtaacggt gttctcggcg gtgaacatgg agcgccccct
     1981 ttggcggtgg cgaggagtat gcacctcgtc agcacgtgcg tggtgcgtct cgtacactcg
     2041 ctgaggtgag ccgcctcgga cggtccgagc ggttccctgc ctgaggcgcg catgcttact
     2101 cgagggttcg tggtcctgtg gatcgcgatg gcggtcgcta tggccggcat cggcatggtg
     2161 tcgccgttgc tgccggtgta cgtgcgcgag gagttgcacg gtcccgagct ggcggtcgcg
     2221 ctgtcgttct cggggctcgc ggtgacgcag ctggcgatgt cgccactggt gggacggctg
     2281 ggcgaccggc acgggccaaa gatcctgatc gtcgtcggct tcctcatcta cggtgcggcg
     2341 ggcctcggat acctgttttc gcaccactgg gcgctcgtca tcgcgttccg gctgctctcg
     2401 ggagtggggg cggcggcgat cttcccgatg gcgctgtcgt acgtgggccg gctcgcgccg
     2461 ccggggcgcg aagggacgta catggggatg tacgccgtcg cggagacggc ggggttcggg
     2521 ctgggtcctc tgctgggcgg cgcggtgcgc gacctcgtga gctcgcaggc ggcgttcctg
     2581 acgatgtcag tggcgctctg cgggacgggg ctgctggcac tgatcgcgct gccggcggct
     2641 gcctatgccg gacaaccgtt cttcagcagc gcgctacgcg ccctccgcgc acgccaagtg
     2701 aacatggacg tgccgatctc gctcggcgtg ctgctcgcac tcggcatgtc ggtcgtcgaa
     2761 ac
//
LOCUS       DAORPG010000222         2762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3876_length_2762_cov_3.436646, whole genome shotgun sequence.
ACCESSION   DAORPG010000222 DAORPG010000000
VERSION     DAORPG010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3876_length_2762_cov_3.436646"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..1522
                     /locus_tag="PLX85_05795"
     CDS             314..1522
                     /locus_tag="PLX85_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013476049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62720.1"
                     /translation="MSGGRLILGVAALSLPAIFLRVTGTHTGTVTDTAIFGLAIVAAA
                     FILAWAAEAAEVDISQALAVAFVALIAVLPEYAVDMTFAWKAGQDPSFAPYAVANMTG
                     GNRLLIGAAWPMVFFLFWRRARVPVMALEHGHAIEIATLAVATLYSFTIPLKGEIALY
                     DTAILGALFVGYVYVIARAPTEEPELIGPARTIGMLPTYQRRGSIVLLLLYAAGAIFA
                     SAEPFADGLVHTGVQLGIDEFLLVQWLAPLASEAPEFLFAGILANRGRAGVSMGALLS
                     SKVNQWTLLIGGLPLAYAISSGGAHGLPLDTRQVEEVLITAAQSAFAVAILASLTITK
                     WEAILLFGLFAIQFAIPVEQIRMVIAAIYIVLAVAILLRQRSEVARLLRLSRRTIAGH
                     HDELAAEAAD"
     gene            complement(1542..>2762)
                     /locus_tag="PLX85_05800"
     CDS             complement(1542..>2762)
                     /locus_tag="PLX85_05800"
                     /inference="COORDINATES: protein motif:HMM:NF023288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramidase domain-containing protein"
                     /protein_id="HRC62721.1"
                     /translation="APAARRLSTGVGALSAAVASAWNAYGGLLDALCRVVGVDPAAIA
                     AVMHTESGGRGMGPDGRMIIRFENHLFRRYLGDERASLFDAHFRYDPATVWQGHQFCR
                     EGDAWQPCHQSQAGEWEVLEFARSLDETAALMSISMGVAQIMGFNHMLIGYDSVREMF
                     DRFSTDIRFQILGMFDFIRGHGDSSPMLEALRVRNYEAFATGYNGNGQAAFYGGLISS
                     AVAAFEQLQGSPAQPPAPGDATEGVYVVQPGDSLGRIAARFGTTAAALAAANGIANLD
                     LIAPGQRLRIPGRPTVAEPPPPPPVADQTYTVQPGDILGRIAARFGVPLQAIIEANGI
                     ANPDSIQAGQVLRIPPATQPRELRPPAVRHREHLVLAGDTLRSVAAELGATPQALAAV
                     NDLQLIPGQLLRAP"
BASE COUNT          419 a          835 c         1064 g          444 t
ORIGIN      
        1 ggaagtcccg aacccggggc acccgtatgg ggtgcgcggg gtggcggaag tgccgatcgt
       61 cccggcgctg gcggcggtcg cgaacgcgat ctaccacgcg acgggcgtgc gggtgcagaa
      121 gctgccggca tcgcctcggg cgttgctgga ggagctgctc gacaacccgt cgtagcgcac
      181 attcgaggcc aaagcgaagc cggggaggcc ttcctcctcg gcttcgctgt tcttcagcgg
      241 cggcggatgg gatgcgcgca gtagccgagc gggtatgctc ggacgcgaac cagcgagcta
      301 gtggggagca agcgtgagcg gcggtcgtct cattctcggc gtggccgcgc tctcgttgcc
      361 tgccatcttc ctgcgcgtta cggggacgca cacggggacc gtcacggaca cggcgatctt
      421 cgggctggcg atcgtggcag cggcgttcat cctcgcgtgg gcggctgagg cggccgaggt
      481 cgacatctcg caggcgctcg cggtggcctt cgtggcgctg atcgcggtgc tgcctgagta
      541 cgcggtggac atgaccttcg cctggaaagc ggggcaggac ccctcgttcg cgccctacgc
      601 ggtggcgaac atgaccggtg gcaaccggct gctgatcggc gccgcctggc cgatggtctt
      661 cttcctgttc tggcgccgcg cgcgggtgcc agtgatggca ctggagcacg ggcacgcgat
      721 cgagatcgcg acccttgccg tggccacgct gtactcgttc accatcccgc tcaaaggcga
      781 gattgcgctc tacgacacgg cgatcctcgg ggcactgttc gtcgggtacg tgtacgtgat
      841 cgcgcgggcg ccgacggagg agccggaact gatcgggccg gcacgaacga tcgggatgct
      901 gccgacgtat cagcgccgag gaagcatcgt gttgctgctg ctgtacgcgg cgggagcgat
      961 cttcgcctcg gcggaaccgt tcgcagacgg tctcgtgcat acgggcgtgc agctcggaat
     1021 tgacgagttc ctgttggtgc agtggctcgc cccgctggcc tcggaagcgc cagagttcct
     1081 gttcgccggc atcctggcga accggggacg agcgggcgtg tcgatggggg cgctgctgtc
     1141 ctcgaaggtc aaccagtgga cgttgctcat cggcgggctg ccactggcgt atgcgatctc
     1201 gagtggaggg gcccacgggc tgccgctgga cacgcggcag gtggaagagg tgctcatcac
     1261 cgccgcacag tcggcgttcg cggtggcgat cctcgcgagc ctgacgatca cgaagtggga
     1321 agcgatcctg ctcttcgggc tgttcgcgat tcagttcgcg attccggtgg agcagatccg
     1381 gatggtgatc gcggcgatct acatcgtgct cgcggtggcg atcctgctgc ggcagcgatc
     1441 ggaagtggcg cggttgttgc gtctgtcgag gcggacgatc gcgggacatc acgacgagct
     1501 ggcagccgaa gcagccgact gactccgccg cgcgcctgcc gttacggcgc gcgcagcagc
     1561 tgaccaggga tgagctgaag gtcgttcacg gcggcgaggg cctgcggcgt cgcaccgagt
     1621 tcggccgcga ctgagcgcag cgtgtcgccg gcgagcacca ggtgctcgcg gtggcggaca
     1681 gcggggggac ggagctctcg aggctgggtg gcgggcggaa tgcgcagaac ctgtccggcc
     1741 tgaatcgagt ccgggttcgc gatcccgttc gcctcgatga tggcctgcag gggaacgccg
     1801 aagcgggcgg cgatgcggcc gaggatgtcg ccgggctgga cggtgtaggt ctgatctgcc
     1861 acggggggtg ggggaggcgg ctccgcgacc gtggggcgac cggggatgcg gaggcgctgg
     1921 ccgggcgcga tgaggtccag gttcgcgatg ccgttggcgg cggcgagcgc ggcggcggtc
     1981 gtaccaaagc gcgcggcaat gcggcccaga ctgtcgccgg gctggacgac gtagacaccc
     2041 tcggtggcgt cgcccggcgc gggcggttgg gcgggcgagc cctggagctg ctcgaacgcc
     2101 gcgacggccg agctgatgag gccgccgtag aaggcggctt gcccgttgcc gttgtacccg
     2161 gtcgcgaacg cctcgtagtt gcggacgcgg agcgcctcga gcatgggcga ggagtcgcca
     2221 tgacctcgga tgaagtcgaa catgccaaga atctgaaagc ggatatcggt gctgaagcgg
     2281 tcgaacatct cgcgaacgct gtcgtagccg atcagcatgt ggttgaagcc catgatctgt
     2341 gcgacgccca tcgagataga catgagggcg gcggtctcat cgaggctgcg ggcgaactcg
     2401 aggacctccc actcaccggc ctgactctgg tggcagggct gccaggcatc gccttcacgg
     2461 cagaactggt ggccctgcca gaccgtggcg gggtcgtagc ggaagtgggc gtcgaagagg
     2521 ctggcgcgct cgtcgccgag atagcggcgg aagaggtggt tctcgaagcg gatgatcatc
     2581 cggccgtcgg ggcccatacc gcgcccgccc gactcggtgt gcatgacggc ggcaatcgcg
     2641 gctggatcga cacccacgac gcgacagagt gcatcgagga ggccaccgta ggcgttccac
     2701 gccgaggcaa cggcggctga gagagcgccg acgcccgtac tgaggcggcg ggcggccggg
     2761 gc
//
LOCUS       DAORPG010000223         2754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3898_length_2754_cov_3.007040, whole genome shotgun sequence.
ACCESSION   DAORPG010000223 DAORPG010000000
VERSION     DAORPG010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3898_length_2754_cov_3.007040"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1224)
                     /locus_tag="PLX85_05805"
     CDS             complement(133..1224)
                     /locus_tag="PLX85_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014694559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC62722.1"
                     /translation="MRAFTHRLTATLAFATAVLFVAATVAACGGDDTANTGPGASATA
                     KVENVTLRLGYFPNITHAQPLVGLERGTYAEELGANVKLETKTFNAGPAAIEALFAGA
                     IDATYIGPNPAINGYVQSEGKDLRIVAGATSAGASLIVRADAGMSTPADFANKKVATP
                     QLGNTQDVALRAWLQKNGLKDKDHGGNVQVIPTANADTLTLFQRGQIDAAWVPEPWGT
                     RLIQEANGRLFLDERDLWPNGDFVTTQLIVRTKFLNEHPEVVEKLLLAHVKTTLWIND
                     NPDEAKKLVNQGIKKVTNAAIPDAVINTAWRNQKVTYDPIASSLRKSADDAFALGYLG
                     DKKPDLANIYSLDLLNKVLASLNLKAVQQ"
     gene            complement(1400..1567)
                     /locus_tag="PLX85_05810"
     CDS             complement(1400..1567)
                     /locus_tag="PLX85_05810"
                     /inference="COORDINATES: protein motif:HMM:NF021566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HRC62723.1"
                     /translation="MAGDRDATALDPRERQVLAEVLQAIRSVRHGYVQITLQDSKVVQ
                     IDTLEKRRFER"
     gene            complement(1567..2031)
                     /locus_tag="PLX85_05815"
     CDS             complement(1567..2031)
                     /locus_tag="PLX85_05815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRC62724.1"
                     /translation="MKLSTRGDYAVRALLELSTADADNAIPLGELAERTRIPAKYLEQ
                     ILMRLRTARVVRGKRGARGGYMLARDPHRVTVGEVIRAMEGPLAPSLCASQTAHTACP
                     AYRCPSEDACVMRGLWAEVRDAIAGIVDHTTFGDLAARTREQQAAAREMYYI"
     gene            complement(2118..2609)
                     /locus_tag="PLX85_05820"
     CDS             complement(2118..2609)
                     /locus_tag="PLX85_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62725.1"
                     /translation="MATSNARSGVRLELLWALATIVSVLAVLTWRCALDPGATSSAGS
                     AAAETPAATPFAPCGDEVNRPDEPLNLEGRACLLTAAPAGTRVEFNTTRFTVEGQPVY
                     WRIRVVAWGDVEVTVDNRADEFSGAARRRVTTYHCTSLARSAADGQRIDVLGCTSGDV
                     LTF"
     gene            complement(2620..>2754)
                     /gene="pheS"
                     /locus_tag="PLX85_05825"
     CDS             complement(2620..>2754)
                     /gene="pheS"
                     /locus_tag="PLX85_05825"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483641.1"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 1 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRC62726.1"
                     /translation="AGYDTDRYTGFAAGMGVERITMLKYGVEDIRHFYSNDLRFLAQF
                     "
BASE COUNT          407 a          803 c         1058 g          486 t
ORIGIN      
        1 accgtcgggg cggccgtcgc ccgccgtgca ggaggagggg cgacggccgc ctcgacggcg
       61 ggatcggcgc tgtcctcgat gtcgggctgc gtgacgatga ccgtcatggc gacttcctcc
      121 cgcagatggt ggctattgct gtacggcttt gagattcagg ctcgcgagga ccttgttgag
      181 gagatcgagc gagtagatgt tcgccaggtc gggcttcttg tcgccaaggt agccgagggc
      241 gaaggcgtca tcggccgact tgcgcaggct cgaggcgatg gggtcgtagg tgaccttctg
      301 gttgcgccag gcggtgttga tgacggcatc cgggatcgcg gcgttggtga ccttcttgat
      361 gccctgattg acgagcttct tggcctcgtc cgggttgtcg ttgatccaga gcgtggtctt
      421 tacatgggcg agcaggagct tctcgacgac ctcggggtgc tcgttgagga acttggtgcg
      481 gacgatgagc tgggtggtga caaagtcgcc gtttggccag aggtcgcgtt cgtcgaggaa
      541 gaggcgcccg ttcgcttcct ggatgagacg cgtgccccag ggctccggga cccaggcggc
      601 gtcgatctgt ccgcgctgga agagcgtgag cgtgtcggcg ttggcggtcg ggatgacctg
      661 gacgttgcca ccgtgatctt tgtccttgag gccgttcttc tggagccagg cgcggagggc
      721 gacgtcctgg gtgttgccga gctgaggcgt cgcgaccttc ttgttcgcga agtccgctgg
      781 cgtgctcatg ccggcgtcgg cgcgcacgat gagggacgct ccggcgctgg tagcgccggc
      841 gacgatgcgg aggtctttgc cctcggactg cacgtagccg ttgatcgcgg ggtttgggcc
      901 gatgtaggtg gcgtcgatcg cgcccgcaaa gagcgcctcg atggcggccg ggccggcgtt
      961 gaaggtcttc gtctcgagct tcacgttggc gccgagttcc tcggcgtagg tgccgcgctc
     1021 gaggccgacg aggggctggg cgtgggtgat gttcgggaag tagccgaggc ggagggtgac
     1081 gttctcgacc ttcgcggtgg ccgaggcgcc cgggccggtg ttggcggtgt cgtctccgcc
     1141 gcaggcagcg acggtcgcgg caacgaagag gacggcggtg gcgaacgcca gtgtggcggt
     1201 caggcggtgc gtaaacgcgc gcatgtgctt actgctccag tcggtcgtgc gtcgagtcgt
     1261 tggttgcggg tcgccctcgc ggcgcgaggg ccccgtacag cgaccttgaa cgggcaacga
     1321 aaagcccctc gctgttcgcg aggggcctcc agttgactgg tcagcccgcg cccgtacgcg
     1381 cggcggaggt ggcatagcgt cagcgttcaa agcggcgttt ctcgagcgtg tcgatctgga
     1441 cgactttcga atcctgcagc gtgatctgta cgtagccgtg ccggaccgaa cggatggcct
     1501 gcagcacctc ggcgaggacc tggcgctcgc gcggatcgag ggcggtggca tcgcggtcgc
     1561 ctgccatcag atgtagtaca tctcgcgcgc ggcggcctgc tgctcgcggg tgcgggccgc
     1621 gaggtcgcca aaggtggtgt ggtcgacgat gccggcgatg gcgtcgcgaa cctcggccca
     1681 gaggccccgc atcacacagg cgtcttcgct ggggcaacgg taggcggggc atgcagtgtg
     1741 ggcggtctga ctcgcgcaga ggctgggcgc cagcgggccc tccatggcgc ggatcacctc
     1801 gccaacggtg acgcggtggg ggtcgcgggc gagcatgtac ccgccacgtg ccccgcgctt
     1861 tcctcgcacg acgcgggccg tgcgaaggcg catcaggatc tgctcgaggt acttcgcggg
     1921 gatgcgggtg cgttcggcga gctctccgag ggggatggcg ttgtccgcat ccgccgtcga
     1981 gagctcgagg agggcgcgaa cggcgtaatc accgcgggtg gaaagcttca ttcgacaatc
     2041 cctatccagt tagtaggttt tatctgagca aaggctgact gtcaagcgta gctcccgaac
     2101 agcaggccct gcgtgtctca gaaggtcagg acgtcgccgc ttgtgcagcc gagaacgtcg
     2161 atgcgctgcc cgtccgccgc agaacgggcg agcgaggtgc agtggtaggt cgtgacgcgc
     2221 cgacgggcgg cgcctgagaa ctcatcggca cggttgtcga cggtcacctc gacgtcgccc
     2281 caagcaacga cccggatgcg ccaatagacg ggctggccct cgaccgtgaa gcgggtggtg
     2341 ttgaactcga cccgggtacc ggcgggagcg gctgtgagca ggcaggcacg accctcgagg
     2401 ttcagaggct cgtccggacg gttgacctcg tcgccgcacg gcgcgaaggg ggtggcagcg
     2461 ggcgtctccg cagcagcgga gccggcggaa ctcgtcgcgc cgggatcgag ggcgcagcgc
     2521 caggtgagca cggcgaggac ggagacgatc gtggcgaggg cccagagcag ctcgaggcgg
     2581 acaccggagc gagcgttgct ggtggccacc gaggcgcggt cagaactgcg cgaggaagcg
     2641 gaggtcgttc gagtagaagt ggcggatatc ctcgacgccg tacttcagca tcgtgatgcg
     2701 ctcgacgccc atgcccgccg cgaagccagt gtagcggtcg gtgtcgtagc cggc
//
LOCUS       DAORPG010000224         2750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3908_length_2750_cov_3.553618, whole genome shotgun sequence.
ACCESSION   DAORPG010000224 DAORPG010000000
VERSION     DAORPG010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3908_length_2750_cov_3.553618"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLX85_05830"
     CDS             <1..202
                     /locus_tag="PLX85_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002849199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HRC62727.1"
                     /translation="GNRRLYSHRDIDRLRQVLRLVGDLGVNLAGVDVILRMTRQIEEL
                     QLQLAEAHAEIARLRAAQDGLR"
     gene            295..2739
                     /locus_tag="PLX85_05835"
     CDS             295..2739
                     /locus_tag="PLX85_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC62728.1"
                     /translation="MRQDRFTEQAQAVLQASQEMVRQQRHTQWDVEHVLLALVQTRDG
                     LAKDVLQKMGVNPQAVASRLAGYLDRSPKAEFEVVQIYTTPRIVRMLEAANAEAERLQ
                     DEYVGVEHLLIAISDEREGEAARVLNEFQITKERIYNALRQVRGSARVSSPNAESQYQ
                     ALARYSVDLTQLARDGKLDPTVGREDEVARVMQILNRRTKNNPVLIGEAGVGKTAIVE
                     GLASRIVHDEVPERLRGKRLLALDIGQLVAGSKFRGEFEERLQAVMREVREAAGEVLL
                     FIDEIHQVVGAGGAEGAIDASTMMKPALARGELHVVGATTLEEYRRYIEADPALERRF
                     SPVFVDEPTVEEAIEILRGLRPKYEEHHGLRISEEAVDAAVRLSSRYLTERKLPDKAI
                     DLIDEAASKHVIEAESSPPEVRDLRERLTEAQERLDAAAARQDFEEAARIKQQVLQVQ
                     SEYEERARAWRAERGITEEVTAQDIAALIAQMTGIPVDKMLEGEAEKLLHMEEGLHQR
                     VVGQDAAIVALADAIRRARAGLKDPRRPIGSFIFLGPTGVGKTELAKALASYLFDDED
                     AIVRLDMSEYQERHTVSRLVGAPPGYVGYDEAGGLTEQVRRRPYQVILFDEIEKAHPD
                     VFNTLLQILEDGRLTDSHGRTADFRNTVIILTSNLGTGAAAHGPIGFQARARTEAERD
                     ARHRNVEDALKQSFRPEFLNRIDEIIVFEPLTETELAQVADLLLDEVRERLEERQVDF
                     EVTTRAKEALVKEGYDPAFGARPLRRTIQRRVENPLAKRVLSGEFADGDRVLVDLDDA
                     GEYTFRRAESREPVAV"
BASE COUNT          473 a         1027 c          884 g          366 t
ORIGIN      
        1 gggcaaccgc cgcctgtact cgcaccgcga catcgaccgg ctgcgccagg tgctccgcct
       61 cgtcggcgac cttggcgtca acctcgccgg tgtcgacgtc atcctccgga tgacgcgcca
      121 gatcgaggag ctccagctcc agctcgccga agcccacgcg gagatcgccc ggctccgcgc
      181 ggcccaggac ggccttcgat gaaggaggcc cgctggctcg ccgggtgaag attcaagcga
      241 ccacgacaca gggaaagacg aggcgcgaac cgcctcgggg gtgatgcacc aatgatgcga
      301 caggaccgat tcaccgagca ggcccaggcc gtgctgcagg cgtcccagga gatggtgcgc
      361 cagcagcgcc acacgcagtg ggacgtcgag cacgtcttgc tcgcgctcgt ccagacacgc
      421 gacggcctcg cgaaggatgt cctccagaag atgggcgtca acccgcaggc cgtcgcctcg
      481 cggctcgccg gctacctcga ccgctccccc aaggcggagt tcgaggtcgt tcagatctac
      541 acgacccctc gcatcgtccg catgctcgag gccgccaatg ccgaggccga gcgcctgcag
      601 gacgagtacg tgggtgtcga gcacctgctg atcgccatct ctgatgagcg cgagggcgag
      661 gccgcacgcg tgctcaacga gttccagatc accaaggagc gcatctacaa cgcccttcgc
      721 caggtccgag gcagcgcgcg cgtctcctcg cccaacgcgg agtcgcagta ccaggcgctc
      781 gcccgctact cggtcgacct cacccagctc gcccgcgacg gcaagctcga ccccaccgtg
      841 ggccgcgagg acgaggtcgc gcgcgtgatg cagatcctca accgccgcac gaagaacaac
      901 cccgtcctca tcggcgaggc cggcgttggc aagaccgcca tcgtcgaggg cctcgcgtcc
      961 cgcatcgtcc acgacgaggt ccccgagcgc ctgcgcggca agcgcctgct cgcgctcgac
     1021 atcggccagc tcgtcgccgg ctcgaagttc cgcggcgagt tcgaggagcg tctgcaggcc
     1081 gtcatgcgcg aggtgcgcga ggccgccggt gaggtgctgc tcttcatcga cgagatccac
     1141 caggtcgtcg gcgccggcgg cgccgagggc gccatcgacg cctccacgat gatgaagccc
     1201 gcgcttgccc gcggtgagct ccacgtcgtc ggcgctacca cgctcgagga gtaccgccgc
     1261 tacatcgagg ccgaccccgc cctcgagcgc cgcttctccc cagtcttcgt cgacgagccc
     1321 acggtcgagg aggcgatcga gatcctccgc gggctgcgcc cgaagtacga ggagcaccac
     1381 ggcctccgca tctcggagga agccgtcgac gcggccgtcc gcctctcctc gcgctacctc
     1441 accgagcgca agctgcccga caaggcgatc gacctgatcg acgaggccgc ctcgaagcac
     1501 gtcatcgaag ccgagtcgag cccgcccgag gtccgcgacc tgcgggagcg gctgaccgag
     1561 gcgcaggaac gcctcgacgc cgccgccgcg cgccaggact tcgaggaagc agcccgcatc
     1621 aagcagcagg tgctgcaggt gcagtcggag tacgaggagc gtgcccgcgc atggcgcgcc
     1681 gagcgcggca ttaccgagga ggtgacggcg caggacatcg ccgccctcat cgcccagatg
     1741 accggcatcc ccgtcgacaa gatgctcgag ggtgaggccg agaagctgct tcacatggag
     1801 gaaggcctgc accagcgggt cgtcggacag gacgccgcca tcgttgccct cgccgacgcc
     1861 atccggcgcg cgcgcgccgg cctcaaagat ccgcgccggc ccatcgggtc gttcatcttc
     1921 ctcggcccca cgggcgtcgg gaagaccgag ctcgcaaagg ccctcgcctc gtacctcttc
     1981 gatgacgagg acgccatcgt tcggctcgac atgtccgagt accaggagcg ccacaccgtc
     2041 tcacggctcg tcggcgcccc tcccggctac gtcggctacg acgaggcggg gggcctcacc
     2101 gagcaggtgc gccgccgtcc ataccaggtg atcctcttcg atgaaatcga gaaggcccac
     2161 ccggacgtct tcaacacgct gctgcagatc ctggaggacg gccgcctgac cgactcgcac
     2221 gggcgcacgg ccgacttccg taacacggtg atcatcctca cctcgaacct cggtaccggc
     2281 gcggccgccc acggccccat cggcttccag gcccgtgccc gcaccgaggc ggagcgcgat
     2341 gcgcgccatc gcaacgtcga ggacgccctg aagcagtcgt tccggcccga gttcctgaac
     2401 cgcatcgacg agatcatcgt cttcgagccg ctcaccgaga ccgagctggc gcaggtcgcc
     2461 gatctgctcc tcgacgaggt ccgcgaacgc ctcgaggaac gccaggtcga cttcgaggtg
     2521 accacgcgcg caaaggaagc gctcgtgaag gaaggctacg accccgcctt cggcgcccgc
     2581 cccctgcggc gcacgatcca gcgtcgagtc gagaaccccc tcgccaagcg cgtcctctcc
     2641 ggcgaattcg ccgacggcga ccgcgtgctg gtcgacctcg acgacgccgg ggagtacacc
     2701 ttccgccgcg ccgaatcgag ggagccagtc gccgtctagc ctcaacgggc
//
LOCUS       DAORPG010000225         2747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3915_length_2747_cov_3.215082, whole genome shotgun sequence.
ACCESSION   DAORPG010000225 DAORPG010000000
VERSION     DAORPG010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3915_length_2747_cov_3.215082"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="PLX85_05840"
     CDS             complement(<1..1021)
                     /locus_tag="PLX85_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62729.1"
                     /translation="MRSFLVGRVAVLAFAAGVSVLALARTGEAQTTDLFAGTVDAGFE
                     GSGEGWEALGGGTLVVDDSGPVHGGTQAARIEASDLQAAGIRSQYWLTPTVAGATHTL
                     GLWLYDDDPLTSALRVELEFLDSGGRRRGGLVTELGNGTSGWRLVTLTQVAPDGTAYV
                     RTSARAEVSGAGARFYVDDVSLERGAAPFITPSATASPSPTASATSTVSPTSTPSSTP
                     SPPATPTATATASVPRVFDVLTNGGFEDGLFGWADVGADVEAVGGAALVRSQSTSTKY
                     LYQIVRVSPGGWYEASAYLEAGAGVDAAWVRIAWYASGDGGGSQLATGDAPPLNGGSG
                     AKLLGM"
     gene            1180..2034
                     /locus_tag="PLX85_05845"
     CDS             1180..2034
                     /locus_tag="PLX85_05845"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRC62730.1"
                     /translation="MDIKRVGVIGGGIMGSGIAQVCASHGYEVTVVEVDDSRAQAAVS
                     GIASRLDREVQRERLTRERHDEILGQLQAHADLGRVADCDLVIEAIVEDLPTKQDLFA
                     RLGAVCKPDAILATNTSSVSVSALAAASGRPEQVLGIHFFNPPTVLPLAEIISTGSTS
                     AATVEAARAFCESIDRVIVFAKDRPGFIVNRLLIPFILDAIRLVDHGIASAQEVDDGF
                     RAGANHTMGPIATADLIGLDTVAHIADTLFEELGESRYKTPPVVTRMIALGHLGRKSG
                     KGFFDYAR"
     gene            complement(2067..2600)
                     /locus_tag="PLX85_05850"
     CDS             complement(2067..2600)
                     /locus_tag="PLX85_05850"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015170722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRC62731.1"
                     /translation="MNAHRFASTVRWPLFAGALLAALLVACGGDDAKSAATSTATAKT
                     EASSVASSDGNAPGIPVLTGQVQRTASGLGYIDEKVGDGAAPQKGQQVTVHYTGWLTS
                     GKKFDSSRDRGQPFVFPIGAGRVIAGWDEGVATMKVGGKRRLIIPANLGYGARGAPPS
                     IPANAVLIFDVELLGVQ"
BASE COUNT          423 a         1000 c          913 g          411 t
ORIGIN      
        1 acatgcccag cagcttcgcg cccgagccgc cgttgagcgg cggcgcgtcg cccgtggcaa
       61 gctggctccc gcccccgtca cccgaggcgt accaggcgat ccgcacccac gccgcgtcta
      121 cccccgctcc cgcttcgagg tacgcgctcg cctcgtacca accgccgggc gacacccgca
      181 caatctggta gaggtacttc gtcgaggtcg actgcgaccg cactagggcc gccccgccga
      241 cagcctcgac gtccgcgccc acgtccgccc atccaaatag accgtcctcg aagccgccgt
      301 tggtgaggac gtcgaacacg cgtgggacgg aagccgtcgc ggtggccgtg ggagtcgccg
      361 gcggtgacgg cgtcgacgag ggcgtgctcg tgggcgagac cgtcgaggtc gcactcgccg
      421 tgggactcgg gctcgccgtg gcgctcggcg tgatgaaggg ggccgcgcct cgttcgagcg
      481 acacgtcatc gacgtagaag cgggcgcccg cgccgctgac ctcggctcgc gccgaggtgc
      541 gtacgtacgc ggtgccgtct ggagccacct gggtcagtgt gacgaggcgc caccccgagg
      601 tgccgttgcc cagctcggtc acgaggccgc ctcgccgccg cccgccggag tcgaggaact
      661 cgagttctac ccgcagcgcc gaggtgagcg ggtcgtcgtc gtacaaccac agaccaagcg
      721 tgtgcgtcgc cccggcgacg gtgggcgtca gccagtactg cgagcggatg cctgccgcct
      781 gcaggtcgct cgcctcgatc cgcgcggcct gcgtcccgcc atggacgggc ccgctgtcat
      841 cgacgacgag ggtgccgccg ccgagagcct cccatccctc gccgctgccc tcgaagccgg
      901 catccaccgt ccccgcgaac aggtcagtcg tctgcgcctc gcccgtccgg gccagcgcga
      961 gcaccgaaac gccggccgcg aacgccagca cagccacgcg ccccacgagg aagctgcgca
     1021 cgtcgaggcg cctgcctttc ccggctgggg atggggtccc gcgaccgcgc cgcgaaggcc
     1081 gcgagcacag ctgtaatagc acagaacgcg gaccgcgggc gtatcgtgtg gccgcgaacg
     1141 cgcggaaaac ggcacggcgt atccggaagg agccgcgagg tggacatcaa gcgagtgggc
     1201 gtcatcggtg gcggcatcat gggctcaggc atcgcccagg tctgcgcctc gcacgggtat
     1261 gaggtcacgg tggtcgaagt ggacgattcc cgcgcgcagg ccgccgtcag cggcatcgct
     1321 tcgcgactcg atcgcgaggt acagcgcgag cgcctcaccc gcgaacgcca cgacgagatc
     1381 cttggccagt tgcaggccca cgccgacctc ggtcgcgtcg ccgactgcga cctcgtgatc
     1441 gaggcgatcg tcgaagacct cccaacgaag caggacctct tcgcccgcct cggcgccgtt
     1501 tgtaagccgg acgcgatcct cgccacgaat acctcgtccg tgtccgtctc agcgctcgcg
     1561 gcggcgtcgg gccggcccga gcaggtcctc ggcatccact tcttcaaccc gccgaccgtg
     1621 cttccgctcg ccgaaatcat ttcgaccggc agtacctcgg ccgcgacggt cgaggccgcc
     1681 cgcgccttct gcgagtcgat cgaccgagtc atcgtctttg cgaaggaccg ccccggcttc
     1741 atcgtgaacc ggctgctgat cccgttcatc ctcgatgcga tccggctcgt ggatcacggc
     1801 atcgccagcg cgcaggaggt cgacgatggc ttccgggctg gagcaaatca cacgatgggc
     1861 ccgatcgcga ccgccgatct catcggcctc gacaccgtgg cccatatcgc ggacaccctc
     1921 ttcgaggagc tcggggagag ccgctacaag acgccgccgg tggtcacgag gatgatcgcg
     1981 ctcgggcacc tcggccgaaa gtcaggcaag ggcttcttcg actacgcgcg atagtttgag
     2041 aggcgcgatg ggagggacgc gctcggctac tgaacgccca gcaactccac gtcgaagatc
     2101 agcacagcgt ttgcggggat cgagggcgga gcgcctcgcg cgccgtagcc gaggttcgcg
     2161 gggatgatca accggcgctt gccgccgacc ttcatcgtcg ccactccctc atcccaaccc
     2221 gcgattacgc gtccggcgcc gatggggaac acgaacggct gtccgcggtc gcgagaggag
     2281 tcgaacttct tcccgctcgt cagccacccg gtgtaatgga cggtgacctg ctgccccttc
     2341 tgcggggcgg cgccgtcgcc gaccttctca tcgatgtagc ccaggccgct cgccgtgcgc
     2401 tggacctggc ccgtgagtac ggggatcccg ggcgcgttgc cgtcgctcga agccacgcta
     2461 ctcgcctccg tcttcgcggt cgccgtcgag gtcgccgcgg acttcgcgtc gtccccgcca
     2521 caggccacaa ggagtgcggc cagtagtgcg cccgcgaaca gcggccagcg gacggtcgag
     2581 gcgaagcgat gtgcgttcat ctgcgtgcgg atggtgcagg gctcatcagg tgtgtgcaac
     2641 cgccacccgg atgaaaccta cgcggatctc ccgctggtct cgctgcgtcc gccccgctgc
     2701 tcctcgagga accgctcgac gtccgagatc acctcgtcgc tcgatgg
//
LOCUS       DAORPG010000226         2745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3920_length_2745_cov_3.941264, whole genome shotgun sequence.
ACCESSION   DAORPG010000226 DAORPG010000000
VERSION     DAORPG010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3920_length_2745_cov_3.941264"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLX85_05855"
     CDS             <1..343
                     /locus_tag="PLX85_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62732.1"
                     /translation="VSRDLVEAAGFEDPLELAEITPEDEEAIIRALLRYEDSRYEGYL
                     PTFRDAAGYVRHPANRIDPAIVDVVMNRLVERGVLFQAYEETREGNPIRTTRLDRDHP
                     EVQDALGSGPR"
     gene            complement(362..1504)
                     /locus_tag="PLX85_05860"
     CDS             complement(362..1504)
                     /locus_tag="PLX85_05860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC62733.1"
                     /translation="MDLSLTETQELLKRGVEEFIERETPLSSVVSLQQSERGYSDAIW
                     RTAAEVGWLGMLTPEAYGGSGSSFMDAAVVFQALGAGAIPGPLLSSGVIAPLVIEALG
                     TEAQREQYLPALARGELVATFALTEPEWGWGVDRVQLRAEASGDGYRLNGTKLFVYDA
                     HAADVLLVAARTLEGVRVFVVPTTTAGVEVRRLEGLFSAECQVRLTDVQVPASAMLGR
                     TDGGGSAAALEAALLRATPILCAYQVGGAQTAFELSVDYSRRRRQFGQPIGRFQHVQN
                     HIVQLVNHLDGARWTTYEALWKLDAGKPGFEVSAHLAKVCASEGFVHAVNYAHEVHAG
                     VGVMHEYGLTLFTRLSRSLYFALGDPKWHRQRLAALLPDHELSEVA"
     gene            complement(1551..>2745)
                     /locus_tag="PLX85_05865"
     CDS             complement(1551..>2745)
                     /locus_tag="PLX85_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC62734.1"
                     /translation="QFRAEVRAWLAEHVPQVDGDRDSDENYEKYRQLGRELGAKGWLR
                     PAAPVEYGGGGLPVDHAIVIAEEVDRYDLRLPPYYDSGGTLGGASILVWGTEEQKRRM
                     LPPIFRGEVVTWQLLTGPEAGSDLAGTTTDARRDGDEYVLNGEKVFVGGSHGADFLWT
                     IARTDATGERHRNLSWFLIPADLPGIQYRRMELLGDGNEGIGSGYKNTIYFDNVRVPA
                     AQLVGGENNGWQVASTHLELEHGAGGRIGSNRLFRMTREWARQLKREGRPLIEHPDVQ
                     ERLVDVFVQTEVTRLLNLRNYWMNQSHQRMSYEGPQASYYGKMAGLEVSQAMMDIIGP
                     YALTTDEEWDVSRGLVEPYLKSAIVALHPGGTADIQKVIIARRIGVGRDIAERAGDTG
                     GQAAF"
BASE COUNT          398 a         1104 c          821 g          422 t
ORIGIN      
        1 ggtgagccgc gatctcgtcg aggccgccgg cttcgaggac ccgctcgagc tcgccgagat
       61 cacccccgag gacgaggagg cgatcatccg cgccctcctc cgctacgagg actcacgcta
      121 cgagggctac ctgcccacct tccgcgacgc cgccggctac gtccggcacc ccgcgaaccg
      181 catcgacccg gccatcgtcg acgtcgtcat gaatcgcttg gtcgagcgcg gcgtcctgtt
      241 ccaggcctac gaggagaccc gcgagggcaa cccgatccgc acgactcgcc tcgaccgcga
      301 ccaccccgag gtgcaggacg cccttggctc aggccctcgc tgaggcgggc gggccgagga
      361 gttacgcgac ctcagacagc tcgtggtcgg gcagcagcgc cgccagccgc tggcggtgcc
      421 acttcgggtc gccgagcgcg aaatacagcg agcgcgatag ccgcgtgaac agcgtcagtc
      481 cgtactcgtg catcaccccc acgcccgcgt gcacctcgtg tgcgtagttc accgcgtgca
      541 cgaacccctc gctcgcgcac accttcgcga ggtgcgccga cacctcgaac cccggcttcc
      601 cagcgtccag cttccacagt gcctcgtagg tcgtccagcg cgccccatcg agatggttca
      661 ccagctgcac gatgtggttc tgcacgtgct ggaaccgccc gataggctgc ccgaactggc
      721 gccgccgccg gctgtagtcc acggacagct cgaacgccgt ctgcgccccg cccacctggt
      781 atgcgcacag gatcggcgtc gcccgcagca gtgccgcctc gagggcagcg gcggacccgc
      841 cgccgtccgt gcgccccagc atcgccgagg ccggaacctg gacgtcggtc agccgcacct
      901 ggcactcggc gctgaagagc ccctcgagcc gtcgcacctc gacgcccgcc gtcgtcgtcg
      961 gcacgacgaa cacccgcacc ccttccaagg tccgcgccgc cacgagcagc acgtctgccg
     1021 cgtgtgcgtc gtacacgaac aacttcgtgc cgttcaggcg gtacccgtcc cccgaggcct
     1081 cggcacgcag ctggacgcgg tcgacgcccc agccccactc cggctccgtg agagcgaacg
     1141 tcgccacgag ctccccgcgc gcgagcgccg gcaggtattg ctcgcgctga gcctcggtcc
     1201 ccagcgcctc gatcacgagc ggggcgatta cgcccgagga cagcagcggc cccggaatcg
     1261 cccccgcccc gagcgcctgg aatacgaccg ccgcgtccat gaacgaggag ccgctgccgc
     1321 cgtacgcctc cggcgtcagc atccccagcc agccgacctc ggctgccgtg cgccagatcg
     1381 cgtccgagta cccgcgctcg ctctgttgta gcgacaccac cgaggacagc ggcgtctccc
     1441 gctcaatgaa ctcctcgacg cctcgcttga gcagttcctg cgtctccgtc agtgacaggt
     1501 ccatcgccgt ccccttcgcc cgctcggatc gcgcctcgtc acacccgagg tcagaacgcc
     1561 gcttggccgc ccgtgtcccc ggcccgctcc gcgatgtcgc gccccacacc gatgcggcgc
     1621 gcgatgatca ccttctggat gtccgccgtc ccaccggggt gcagcgccac gatcgccgac
     1681 ttcaggtacg gctccacgag gccgcggctg acgtcccact cctcgtccgt cgtcaacgcg
     1741 tacgggccga tgatgtccat catcgcctgc gacacttcga ggcccgccat cttcccgtag
     1801 tagctcgcct gcggcccctc gtagctcatc cgctggtgcg actggttcat ccagtagttc
     1861 cgcaggttga gcagccgtgt cacctcggtt tgcacgaaca cgtcgacaag gcgctcctgc
     1921 acgtcgggat gctcgatcag cggccgcccc tcgcgcttca gctggcgcgc ccactcgcgc
     1981 gtcatgcgga acagccggtt gctgccgata cgcccgcccg ccccgtgctc cagctcgagg
     2041 tgcgtcgagg cgacctgcca cccgttgttc tcgccgccca ccagctgtgc ggccggcacc
     2101 cgcacgttgt cgaagtagat cgtgttcttg tacccgctcc cgatcccctc gttcccgtcg
     2161 ccgaggagct ccatccgccg gtactggatc cccggcaggt ccgccgggat caggaaccac
     2221 gaaaggttgc gatgccgctc acccgtcgcg tccgtgcgcg caatcgtcca caggaagtcc
     2281 gccccgtggc tcccgccgac gaacaccttc tcgccgttga gcacgtattc gtcgccgtca
     2341 cgacgcgcgt cggtcgtcgt ccccgcgagg tcggagcccg cctcgggccc ggtcagcagc
     2401 tgccaggtca ccacctcgcc gcggaagatc ggcggcagca tccggcgctt ctgctcttcg
     2461 gtgccccaga cgaggatgct cgctccgccc agcgtcccgc ccgagtcgta gtacggcggc
     2521 agccgcaggt cgtaccggtc gacctcctcc gcgatcacga tcgcgtggtc cactggcagc
     2581 ccgcccccgc cgtactcgac gggcgccgcc ggccgcagcc atcccttcgc cccgagctca
     2641 cgtcctagct gacggtactt ctcatagttc tcgtccgagt cgcggtcccc gtcaacctgc
     2701 ggaacgtgct ccgcgagcca cgcccgcacc tccgctcgga actgc
//
LOCUS       DAORPG010000227         2745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3921_length_2745_cov_3.423420, whole genome shotgun sequence.
ACCESSION   DAORPG010000227 DAORPG010000000
VERSION     DAORPG010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3921_length_2745_cov_3.423420"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PLX85_05870"
     CDS             complement(<1..718)
                     /locus_tag="PLX85_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HRC62735.1"
                     /translation="MKPVQLQLQGFTAFHDRITVDFSGRRLFAITGPTGAGKSSLLDG
                     MIWALFGRVPRVSNRIGELISHGAMLARVQFEFEAHGHRYRIARARPLKGQTTARLER
                     LLPGGEAVPLADSVNEVNAHVEAILGIDYDTFVKTIILPQGEFSAFLSGDPKDKKTVL
                     SRLLGLDVYRAMMQSANNRAGGARSAADALQQQHEQLEYRTPDALTAIEAQLRDAEAR
                     QQQLSARQAALKQLETAAASY"
     gene            complement(726..1904)
                     /locus_tag="PLX85_05875"
     CDS             complement(726..1904)
                     /locus_tag="PLX85_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HRC62736.1"
                     /translation="MRFLHTADWHVGRTIRGRSRREEFEAVLSEVVEIARAEDVECLI
                     VAGDVWDQRTASPDSDRLVYEMLRRCRDHGIQVVLLAGNHDSPKKLEALGHISELIGV
                     YTQPEVRRPDAGGVITVEGREHTARIAAVPFITEGQYVGAAALMGLQEEWYGSYADGC
                     AHLLRAMCDRLPADTVNVLTAHLFVNGAQYATKDGSEQLLHIGQAYGVHPQGLPATPQ
                     YIALGHIHRPQRIAEAAAPTAYAGSLLQLDFGEVNDTKGVYIVDAVPGRPLNDLRFVE
                     LTAGRRLRELRGNLDVVLARASEVADAHVRVVLDVDRPEPGLAQRVRDQIPAAVDVRL
                     DYPELVTESEERLAHLDPREQFVRYYRAQHSGADPTTELLALFTELYEDAQTAEPVEA
                     "
     gene            complement(1921..>2745)
                     /locus_tag="PLX85_05880"
     CDS             complement(1921..>2745)
                     /locus_tag="PLX85_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005322189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC62737.1"
                     /translation="PVRIEEAEYMSAPATNRPGRGTGVFSPTRAAIPQKTLRTDAWWK
                     SPLIVDLGFAAFVIYATVRAFMRRLRGAIPHVGHLIRGRASEDNEAHRIDWQSQQLTV
                     IGINNLHDRAQRFVVGVVLKRLMALREQQGVNSESARVFVVLDELNKYAPREGHSPIK
                     DVLLDIAERGRSLGVILIGAQQTASEVESRVVANCSYRIVGRLDSAEAQRGEYGFLTP
                     AARARASLLKPGTMIVHQPEIPVPLLVRFPFPAWATRASEVARTSDPFARFELGRV"
BASE COUNT          389 a          813 c         1114 g          429 t
ORIGIN      
        1 ggtatgacgc cgcggcggtc tccagttgct tgagcgcggc ctgccgggcg gacagctgct
       61 gctggcgcgc ctcggcgtcg cgtaattgcg cctcgatggc ggtgagggcg tcgggcgtgc
      121 ggtactcgag ctgctcgtgc tgctgctgca gcgcatcggc cgcggacctt gcgccgcccg
      181 cgcgattgtt ggcggactgc atcatcgccc tgtagacatc gaggccgagg agcctcgaga
      241 ggaccgtctt tttgtccttc gggtccccgc tcaggaaggc gctgaactcg ccctgcggca
      301 ggatgatcgt cttgacgaag gtgtcgtagt cgatgccgag gatcgcctcg acgtgcgcgt
      361 tcacctcgtt gacgctgtcg gcgagcggga ctgcctcgcc gccggggagc agacgctcaa
      421 ggcgtgcggt ggtctgccct ttgagcgggc gcgcgcgggc gatgcggtag cggtggccgt
      481 gggcctcgaa ctcgaactgg acgcgggcga gcatcgcgcc gtgcgagatg agctcaccga
      541 tgcggttgct caccctcggc acgcggccga agagcgccca gatcatgccg tcgaggagcg
      601 aggacttgcc ggcgccggtg gggccggtga tggcgaagag ccggcggccg ctgaagtcga
      661 cggtgatgcg gtcatgaaag gcggtgaaac cctggagctg gagctggacc ggcttcatcg
      721 gggattcatg cctcgacagg ctctgcggtt tgagcgtcct cgtacagctc ggtgaaaagt
      781 gcgaggagct ccgtcgtggg atccgcgccg gagtgctggg cgcggtagta gcggacgaac
      841 tgctcccgcg ggtcgaggtg ggcgagacgc tcctcagact cggtgacgag ctcggggtag
      901 tcgaggcgga cgtcgacggc ggcggggatc tggtcgcgga cgcgctgggc gagcccgggc
      961 tcggggcggt cgacgtcgag gacgacacgg acgtgggcgt ccgcgacctc ggaggcgcgc
     1021 gcgagtacga cgtccaggtt gccgcggagc tcccgcaggc ggcggccggc ggtcagctcg
     1081 acgaaacgga ggtcgttgag cgggcggccg gggacggcgt cgacgatgta gacgcccttg
     1141 gtgtcgttca cctcgccaaa gtcgagctgg agcagggaac ccgcgtaggc ggtgggggcg
     1201 gctgcctcgg cgatgcgctg cgggcgatgg atgtgtccga gtgcgatgta ctgcggcgtg
     1261 gccggcaggc cctgcgggtg gacgccgtac gcctggccga tgtgcaggag ctgctctgag
     1321 ccgtccttgg tcgcgtactg cgcgccgttc acgaagaggt gggcggtgag gacgttcacg
     1381 gtgtcggcgg ggagacgatc gcacatcgct cgcaggaggt gcgcacagcc gtcggcgtag
     1441 ctcccgtacc actcctcctg gaggcccatc agggcggcgg cgccgacgta ctgaccctcg
     1501 gtgatgaagg ggacggcggc gatccgtgcg gtgtgctcgc ggccctcgac cgtgatgacg
     1561 ccgccggcgt cggggcggcg cacctcgggc tgggtgtaga cgccgatcag ctcggagatg
     1621 tggccgagcg cctcgagctt tttcggcgag tcgtgattgc cggccaggag gacgacctgg
     1681 atgccgtggt cgcggcagcg gcgcagcatc tcgtacacga ggcggtcgga gtcgggcgag
     1741 gcggtgcgct ggtcccagac gtcgcccgcg acaatcaggc actcgacgtc ctcggcacgg
     1801 gcgatctcga cgacctcgga gaggacggcc tcgaactcct cgcggcggct gcgtccgcgg
     1861 atggtgcgcc cgacgtgcca gtcggcggtg tggaggaagc gcattcctag acctgcatcg
     1921 ctagacgcgg ccgagctcga agcgggcgaa ggggtcggat gtgcgggcga cctcggaggc
     1981 gcgggtggcc caggcgggga aggggaagcg cacgaggagc ggcacgggga tctcgggctg
     2041 gtggacgatc atcgtgccgg gcttgaggag gctggcgcgg gcgcgggcgg cgggggtgag
     2101 gaagccatac tcgccgcgct gggcctcggc gctgtcgagg cggccgacga tgcggtacga
     2161 gcagttagcg acgacgcgcg actccacctc ggaggcggtc tgctgcgcgc cgatgaggat
     2221 gacgccgagg gagcggccac gctcggcgat atcgagcagc acgtccttga tcgggctgtg
     2281 gccctcgcgg ggggcgtact tgttcagctc gtcgaggacg acgaacacgc gggcggactc
     2341 ggagttcacg ccctgctgct cgcggagggc catgaggcgc ttgaggacga cgccgacgac
     2401 gaagcgctgg gcgcggtcgt ggaggttgtt gatgccgatg accgtgagct gctgggactg
     2461 ccagtcgatg cggtgcgcct cgttgtcctc ggaggcgcgg ccgcggatga ggtggccgac
     2521 gtgggggatg gcgccgcgaa ggcggcgcat gaaggcgcgc accgtcgcgt agatgacgaa
     2581 cgcggcgaag ccgaggtcga cgatcagtgg agatttccac caggcgtcgg tgcgcagcgt
     2641 cttctggggg atcgcggcgc gggtcgggga gaagaccccg gtgccccggc cgggccggtt
     2701 ggttgcaggc gcgctcatgt attcggcttc ctcaattcgg acggg
//
LOCUS       DAORPG010000228         2728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3967_length_2728_cov_3.384212, whole genome shotgun sequence.
ACCESSION   DAORPG010000228 DAORPG010000000
VERSION     DAORPG010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3967_length_2728_cov_3.384212"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2287
                     /locus_tag="PLX85_05885"
     CDS             <1..2287
                     /locus_tag="PLX85_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62738.1"
                     /translation="ARAFGRRLRVEKVRLRENIALEAPQSDAPPLERARVQLGTLLAD
                     PERTVCLGLVTLAVLFNLVFLLPELTSRPPDLNDNVFHWLNLQRANEALTNLHDPTDP
                     WLNQIALGYPVFHYYQHLPYLVPAFLHVVTFLPLEGLFHALNYLLLCSFPVSIYWSMR
                     RFEFDRVESALAAVVSSVISTDGLFGFDFNSYVWAGYGLYTQLWGMLLLPPALALSYR
                     VVRGGGSYLWAVILLSLTVLSHLAFGYVAFASLVVFAALSPSRRELRARGQRVLALAA
                     LTVGVTSYFLVPLALGSEYLNHSVWDPTFKYDSFGAEQILKWLLQGELFDHNRLPVLT
                     LLLALGLVVCIVSWRREERYRAAAALFVVWLVLYFGPKTWGGLLDATPLGSDFYFHRL
                     IAGVHLAGIFLAGIGLAAPWRWVQRRGDRRYMAGLAALAVIACAPVYLERADYLHDNR
                     TFMQQTSAAFDSEQQDLDALIAVLKSAPPGRVYAGLAAQWGGQYRVGAVPMHAILTAH
                     GIDNVGFLWHPWSLNGDIQVLFDERRPEQYDLFNVRYVVAPEGRTFPDFVHLMGSFGR
                     HRLYRVDTTGYFDVVSSDRAFYADKTTFYYAASNWFASAAGSLRLHPTLVFPRQPVPD
                     GMAETAPLTAAQYPSRQQGTSRPIFGTIEAETVEDGRYAAVVDAPNGGIVMLKATYHP
                     GWRAYVDGAPVATRMVMPSYVGIEVAPGRHGVVVEYGPTTQRHLLQFAGILVLAGFAV
                     RRPAWRHLREVMVRLLQRARL"
     gene            2431..>2728
                     /locus_tag="PLX85_05890"
     CDS             2431..>2728
                     /locus_tag="PLX85_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62739.1"
                     /translation="MVKRIEYLKRRGLIPVLTVAAILAVIGGATMVSAHGGDTTKVHV
                     CVPTVPGQPILASTANATGPNNGCFPGYVAADFSTGPAGPSGATGPTGPSGAIGA"
BASE COUNT          400 a          967 c          841 g          520 t
ORIGIN      
        1 ggctcgcgcc ttcgggcggc gcctacgagt cgagaaggtc cgcttgcggg agaatattgc
       61 cctcgaggcg cctcagtccg acgcccctcc gctggagcgc gcgagggttc agctcggcac
      121 gctgctcgcc gaccccgaac gtaccgtctg cctcgggctc gtcacgttgg ccgtgctgtt
      181 caacctcgtg ttcctgctcc cggagctgac cagccgcccg ccggacctca acgacaacgt
      241 ctttcactgg ctgaacctgc agcgagccaa cgaggcgctg acgaatctgc acgatccgac
      301 cgacccctgg ctcaaccaga tcgcgctcgg ctacccggtc tttcactatt atcagcacct
      361 gccctacctc gtgccggcct tcctccacgt ggtcacattc cttcccctcg aaggactgtt
      421 ccacgcactg aattacctgt tgctgtgctc attccccgtt tcgatctact ggtcgatgcg
      481 gcgtttcgag ttcgaccgcg tcgagtcagc actggccgcc gtcgtctcgt ccgtgatctc
      541 gaccgacggg ctgttcggct tcgacttcaa cagctacgtc tgggcaggct acgggctgta
      601 cacccagctc tggggcatgc tgctgctccc ccccgcgctc gccctgtcgt atcgagtggt
      661 tcgaggaggc ggcagctacc tctgggcggt catcctcctg tcgctgaccg tcctcagcca
      721 tctcgcattc ggctacgtcg cgttcgcctc gctcgttgtg ttcgccgcgc tctccccctc
      781 gaggcgtgaa ctccgagccc gcggccagcg tgttctcgcg ctggccgccc tcactgtggg
      841 cgtcacgtcg tacttcctgg tgccgctcgc actggggagt gagtacctca accacagcgt
      901 ctgggatccg acgttcaagt acgactcgtt tggcgccgaa cagatcctga agtggctcct
      961 ccagggcgag ctgttcgacc acaaccggct gcccgtgctc accctgctgc tcgcgctggg
     1021 cctggtcgtg tgcatcgtgt cctggcgccg cgaggaacgc taccgcgcgg ccgctgcgct
     1081 cttcgtcgtc tggctcgtgc tctactttgg cccgaagacc tggggcggat tgctcgacgc
     1141 caccccgctg ggttccgact tctacttcca ccggctgatc gccggagtgc acctcgccgg
     1201 catcttcctc gcgggcatcg ggctcgccgc cccctggcgc tgggtgcaac ggcgagggga
     1261 tcgcaggtac atggccggac ttgccgccct ggccgtgatc gcctgtgcgc cggtctacct
     1321 cgagcgcgcg gactacctcc acgacaaccg cacgttcatg cagcagacga gcgcggcgtt
     1381 cgactctgag cagcaggacc tcgatgcgct gatcgccgtc ctcaagtccg cgcctccggg
     1441 gcgcgtctac gccggactcg ccgcccagtg gggtggccag taccgcgtgg gggcggtgcc
     1501 gatgcacgcc atcctgacgg cccatggcat cgacaacgtg gggttcctct ggcacccgtg
     1561 gtcattgaat ggcgacatcc aggtgctctt cgacgagcgc cgtcccgagc agtacgacct
     1621 gttcaatgtc cgctacgtcg ttgcgcccga ggggcgcacc ttccccgact tcgtccacct
     1681 catgggctcg ttcggccgtc atcgcctgta ccgagtggat acgacgggct atttcgatgt
     1741 cgtttcgtcc gaccgcgcct tctacgccga caagacgacc ttctactacg ccgcctcgaa
     1801 ctggttcgcg agcgcggccg ggagcctccg cctgcacccc acactggtct tcccccggca
     1861 gccggtccct gacggaatgg ctgagaccgc cccactgacg gcggcgcagt atccgagtcg
     1921 ccagcagggg acgtctcggc cgatattcgg gacgatcgag gccgagacgg ttgaggacgg
     1981 tcgctacgcg gcggtagtcg acgcgccgaa tggcggcatt gtcatgctca aggccacgta
     2041 ccacccgggc tggcgagcgt acgtcgatgg cgcgccggtg gccacgcgga tggtgatgcc
     2101 gagttatgtc ggtatcgagg tggcccccgg acgccatggc gtcgtcgtcg aatacggccc
     2161 cacgacgcag cggcatctcc tccagttcgc gggaatcctt gtgctcgcgg gcttcgcggt
     2221 ccggcggccg gcctggcgac acctgcgtga agtaatggtt aggctcctcc agcgagcacg
     2281 gttgtaggcg ggccggtcct gcccccgcga cctgccagcg tgcacgaacc ccgttcccga
     2341 ataactgggt aaatgtttga cagcccggcc gcctctcacg ataatttcgc cgggtgcccg
     2401 cagggtaccg cacgctaagg agggcgcctt ttggtcaaac gaatcgagta cctgaaacgt
     2461 agaggactga taccggtact caccgtcgcc gccatcctcg ccgtgatcgg cggggcgacg
     2521 atggtgagcg cacatggcgg ggacaccaca aaggtccacg tctgcgtgcc cactgtaccg
     2581 ggtcaaccga tcctcgcctc gacggcgaac gcgaccggcc cgaacaacgg ctgtttcccc
     2641 ggttacgtgg cggctgactt cagcaccggg cccgccggtc cgagtggagc aaccggcccg
     2701 accgggcctt caggcgcaat cggtgcaa
//
LOCUS       DAORPG010000229         2722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3986_length_2722_cov_3.009374, whole genome shotgun sequence.
ACCESSION   DAORPG010000229 DAORPG010000000
VERSION     DAORPG010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3986_length_2722_cov_3.009374"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLX85_05895"
     CDS             <1..227
                     /locus_tag="PLX85_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62740.1"
                     /translation="AAQSGLAPRLSQRETVYEFPGEWASTSWVVVDAYGFRSSQSIGA
                     GFDAALARVRAEWTRVYERDGVEVYRRGEP"
     gene            228..947
                     /locus_tag="PLX85_05900"
     CDS             228..947
                     /locus_tag="PLX85_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC62741.1"
                     /translation="MRAVLVIPALNEEATIGGVVREARAHFAGKIVVADNRSSDRTAE
                     AAWAAGALVASEPVAGYGRACMAGAAAAGDAEVLVFMDGDGSDRPGDIGALLAAIEAG
                     ADLAIGVRGGAGVEPGSMTRAARFGNWLCGVLLGALYGRRLHDLSPLKAVRREFFDGL
                     RHDELTYGWTVELLARSLAAGGRVVEVQVGYRRRAGGTSKVSGDLRASVRAGVRILAT
                     VARVAIAERGRRRRTSYRATS"
     gene            complement(1006..1794)
                     /locus_tag="PLX85_05905"
     CDS             complement(1006..1794)
                     /locus_tag="PLX85_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRC62742.1"
                     /translation="MSYENLQLERDGHVAIVRMNRPERLNALNPGLVRDLNDVFDELD
                     AAFPDVRAIILTGNGRGFCSGADLERAIAAANGAPARPAYDPRAIIQLLAPRVRRQSQ
                     PVIAAVNGIAAGAGFSLSLAADIRIASDQARFASIFVKRGIAVDTGSSISLPDLVGLG
                     VAAEMSYTGRLVDAQWALEKRLVNAVVPHEQLMDAALDLAREIAANPPLTVRASKALL
                     VRRSRLEEAIPHEDDAQTALASTEDRIEAMRAFAEKRAPLFHGR"
     gene            1909..2358
                     /locus_tag="PLX85_05910"
     CDS             1909..2358
                     /locus_tag="PLX85_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRC62743.1"
                     /translation="MQDEDSVAALWAAIRAEQAHLRAVIGAAGAIDLTRRPSGGGWSV
                     LEVLRHLLFAEQAHLGRLDAAQLGWSPLGYTPATMREARKLGARDEGEPSVDEVLAEW
                     QAVHERTEQALGQVDEPAAVAALRRNLKHLRAHARSIERTSGGANRA"
BASE COUNT          387 a          786 c         1148 g          401 t
ORIGIN      
        1 tggcggcgca gtccggactg gcgccgcggc tgtcccagcg cgagacggtg tacgagtttc
       61 cgggggagtg ggcgtcgacc tcgtgggtgg tggtcgacgc gtacgggttc cgctcgagcc
      121 agtcgatcgg ggccgggttc gacgccgcgc tcgcgcgggt gcgcgcggag tggacgcgtg
      181 tttacgaacg cgacggcgtc gaggtgtacc gacgaggcga gccgtaggtg cgtgccgtcc
      241 tcgtcatccc cgcgctgaac gaagaggcaa cgattggcgg ggtcgtgcgc gaggcgcgtg
      301 cgcacttcgc gggcaagatc gttgtagccg acaacaggag cagcgacagg accgcggagg
      361 cagcatgggc tgcgggggca ctggtggcct cggagccggt ggcgggctac gggcgggcgt
      421 gcatggcggg ggcagccgcg gcgggcgacg ccgaggtgct cgtgttcatg gatggcgacg
      481 ggagcgaccg tccgggggac atcggggcgc tgctggcggc gatcgaggcg ggggcggacc
      541 tcgccatcgg ggtgcgtggc ggcgcgggcg tcgagccggg gtcgatgacg cgggcggcgc
      601 ggttcgggaa ctggctgtgc ggcgtgctcc tcggcgcgct gtacgggcgg cgactgcacg
      661 acctctcgcc gctgaaggcc gtgcggcgcg agttcttcga tggcctgcgg cacgatgagc
      721 tgacctacgg ctggacggtg gagctgctgg cgcggtcgct ggccgcgggt gggcgggtcg
      781 tcgaggtgca ggtggggtac cgccggcgcg cgggcggcac ttcgaaggtg agcggggatc
      841 tgcgggcgtc ggtacgggcg ggagtacgca tcctggcgac cgtggcgcga gtggcgatcg
      901 cggagcgggg gcgtcggagg cggacgtcct atcgggcgac ctcatagcgc cccgggggag
      961 cggaggtcga ggagggactg agctcagacc gcgggccgga ccgggtcagc gcccgtggaa
     1021 caacggagcg cgtttctcgg cgaaggcgcg catggcctcg atgcggtctt cggtggaggc
     1081 gagggcggtc tgcgcatcgt cctcgtgggg gatcgcttcc tcgaggcggg agcggcggac
     1141 gagaagcgcc ttgctggcgc ggacggtgag cggcgggtta gcggcgatct cgcgtgcgag
     1201 gtcgagggcg gcgtccatca gctgctcgtg ggggacgacg gcgttgacga ggcgcttctc
     1261 gagcgcccat tgcgcatcga cgagccggcc ggtgtacgac atttcggcgg cgacgccgag
     1321 gccgacgagg tccgggagcg agatggagga gccggtgtcg acggcgatgc cgcgcttcac
     1381 gaagatgctg gcaaaccgcg cctggtccga ggcgatgcga atgtcggcgg cgagcgagag
     1441 ggagaagccg gcgcccgccg cgatgccgtt gacggccgcg atgacgggct gggactgccg
     1501 tcgcacgcgt ggggcgagca gctggatgat cgcgcggggg tcgtaggcgg ggcgtgccgg
     1561 cgccccgttg gccgccgcga tcgcgcgctc gaggtcggcg ccggagcaga agccgcggcc
     1621 gttgccggtg aggatgatgg cgcgcacgtc ggggaaggca gcgtcgagct cgtcgaagac
     1681 atcgttgagg tcgcggacga ggccggggtt gagggcattc aggcgttcgg gtcggttcat
     1741 gcggacgatg gcgacgtgtc cgtcgcgctc gagttgcagg ttctcgtagc tcacttcgac
     1801 ctccatcggc ggcgccctcg ggtgccaatg tagcgcatcg acaatcatgc cggtccctgt
     1861 gggcggcggt cgcccgtggt gggcgcgggc cgcggctaga atcgcgggat gcaagacgag
     1921 gactcggtcg ccgccctgtg ggcagcgatt cgtgcggagc aggcgcatct tcgagctgtg
     1981 attggcgcgg cgggagccat cgacctgaca cggcggccga gcggcggtgg gtggtctgtg
     2041 ctcgaggtgc tgcggcacct gctgttcgcc gaacaggcac acctcggaag gctcgatgcc
     2101 gcgcagctgg gttggagccc gctcgggtac acgccggcaa cgatgcggga ggcgaggaag
     2161 ctcggtgcgc gcgacgaggg cgagccgtcc gtcgacgagg tgctggcaga gtggcaggca
     2221 gtgcacgagc ggaccgagca ggcgctcggg caggtcgacg agcccgcggc ggtcgcggcg
     2281 ctgcggcgga acctcaaaca cttgcgtgct catgcgcgat cgatcgagcg gacctcagga
     2341 ggcgcgaaca gggcttaggg gccgtcggag ctgctcggaa ggaggcacag ccagggcaga
     2401 acggcagttc tgctaaggtg tgtcagcagg tgcttgacat ccttcggggg tgggcacata
     2461 gtgtcgatcc cgctcggcat aatggcgtat gcgggtaagc agttaagccc aagatgttgg
     2521 ggctgctcgt cgggacgacc acaagcggtc gtggatgagg gctccgccag ggcaccggca
     2581 cgaaggaccc accgatgcgc atcgctcgcc acttcaccac cgctggacgc agcccgttcg
     2641 agggtatcga gttcgcgccg cgcgactctc ggatcgtgaa cccggacggg tcggcggtgt
     2701 tcgaggcggc gggtatcacg at
//
LOCUS       DAORPG010000230         2722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3987_length_2722_cov_2.316085, whole genome shotgun sequence.
ACCESSION   DAORPG010000230 DAORPG010000000
VERSION     DAORPG010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3987_length_2722_cov_2.316085"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..>2722)
                     /locus_tag="PLX85_05915"
     CDS             complement(79..>2722)
                     /locus_tag="PLX85_05915"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRC62744.1"
                     /translation="VRGQAAGVGPARWFESIAHTPGLHAMVRAGVGELCRAAVDPDVL
                     VAAAERLGQPDLLALAALYAGYLEVLGERGWHPPEARARLAAQALDVHPLAPLVLVDS
                     MQFLEPGEVELVAGLARRSEVWLALDETSGARAAWTLAALEAAVPDARREVLPAAASV
                     EPGFEAFSAADHDAQLREVARSIKRQLADDRTLRPSDVAVTMRQVGPHLAASRRVFRE
                     FDLPLDPAAGERLSERPFGVWVLRLLRLGAHGWRLLDVLDALEGGWFDRARAGIAQGE
                     LDRLRSIGRRPQLWSGLEALRRLPEATRVEVEAGAEAPDAATRDLHPWLATLDRLSAL
                     LDPDVSRSPGEHATGLDAALFGLDGLVRDPEDDVAFTVETAALRRDLDAFRAVDDALG
                     ATALPFAAFVDLLEARLQRPTTLIREAGGVLLAPMHTLHGLRFRRVYVVGLAEGEFPA
                     PRPKAGLLSPATRRALAEADIDLPPESRATEDELWRGAITRALHTSLWRPRFDDRGRQ
                     AAPSYYFVAAARSPVNELPLGASPEDVGSMRELALSLTRRWPDETRRPRAFPAWELVV
                     GQAAAVEQRRRSFATAGRFEGDLPGLDVAPLVGGKVEWSASRLESYLTCPFQFFGHYG
                     LLLRELDEEQSEADAATRGTVIHAILEDALAPLAGRGGTLDERGLAEAIEGLRTRGRA
                     IWEAAPQRYAFGREALWRHQGEQTLRRLERLLHREAARQQEAAITSIAANELILRGPL
                     PGIDPPLLVQARIDRLDRGGDGGVEVIDYKSGQPIPKADLDGGRRLQLQLYALLAGEQ
                     FGTRSLVGRYAYVRPPKGEWLLDNARNDDRQLLEAAVSLAAEVRDEVAAGRFAVAPAG
                     DCPGYCAARTLCRVNHFSRAKTWS"
BASE COUNT          390 a          952 c         1014 g          366 t
ORIGIN      
        1 gcgtcttgcc gctgccgccc cccggggtga cggcgaggtc gacgtcgccc gcctcgacga
       61 cggcgcgttg ctccgccgtc aactccacgt cttggccctc gagaaatggt taacccggca
      121 gagggtgcgc gcggcgcaat agccggggca gtcgccggcg ggagcgaccg cgaagcggcc
      181 cgcggcgacc tcgtcgcgga cctccgcggc gagcgagacg gcggcctcca ggagctggcg
      241 atcgtcgttg cgggcgttat cgaggagcca ttcgcccttc ggggggcgta cgtaggcata
      301 gcgcccgacg agcgaccgag tgccgaattg ctcgcccgcc agcagcgcgt agagctgcag
      361 ctgcagccgg cggccgccat cgagatccgc ttttggtatc ggctgaccgc tcttgtagtc
      421 gatgacctcg acgccaccgt cgccgccacg gtcgaggcga tcgatgcgag cctggaccag
      481 gagcggcgga tcgatgccgg ggagcgggcc tcgcagtatg agctcgttcg cggcgatcga
      541 cgtgatggcc gcctcctgct gacgggctgc ctcacggtga agcagccgtt cgaggcggcg
      601 tagcgtctgc tcaccctggt gccgccagag cgcctcccgg ccgaacgcgt agcgttgcgg
      661 cgccgcctcc cagatcgcgc ggccgcgggt ccttagtccc tcgatggcct ccgccagccc
      721 ccgttcgtcg agggtaccgc cacgcccggc gagcggcgcc agcgcatcct cgaggatcgc
      781 gtggatgacg gtgcctcggg tggcggcgtc cgcttccgac tgctcttcgt cgagttcccg
      841 gagcagcagt ccgtagtggc cgaagaactg aaacgggcag gtcagatagg actcgaggcg
      901 gctggcgctc cattccacct tgccacccac gaggggcgcg acgtcgaggc cggggaggtc
      961 gccctcgaag cggcccgccg tggcgaacga gcggcgtcgt tgctcgacgg ctgcggcttg
     1021 ccccacgacg agttcccagg cgggaaacgc ccggggccgc cgggtctcgt cgggccagcg
     1081 ccgagtgagg ctgagcgcga gttctcgcat cgagcccaca tcctcgggag aggcgcccag
     1141 tggcagctcg ttcacgggcg agcgcgctgc ggcgacgaag tagtacgagg gagcggcctg
     1201 ccggccgcgg tcatcgaagc gcggacgcca gagcgaggtg tgcagcgccc gggtgatcgc
     1261 accccgccac agctcgtcct ctgtggcgcg ggactcgggc ggcaggtcga tgtccgcctc
     1321 ggccagtgcg cgccgtgtcg ccggcgagag gagtccggcc ttcggacgcg gcgccgggaa
     1381 ctcgccttcc gcgaggccaa cgacgtagac cctgcggaaa cgcagcccgt gcagggtgtg
     1441 catgggagcg aggaggacgc cgcctgcctc gcgaatgagc gtcgtcgggc gttggagcct
     1501 cgcttcgagc aggtcgacga acgcggcgaa cggcagcgcc gtggcgccga gcgcatcgtc
     1561 cacggcgcga aacgcatcga ggtcgcgtcg cagggcggct gtctcgacgg tgaaggcaac
     1621 gtcgtcttcc gggtcgcgaa cgaggccatc caatccgaac agcgcggcat cgaggccggt
     1681 cgcgtgctcg cccggcgagc gactcacatc ggggtcgagt aacgcggaca ggcggtcgag
     1741 ggtggcgagc cacgggtgca ggtcccgggt ggccgcatcg ggggcctccg cgccagcctc
     1801 gacctccacg cgggtcgctt ccgggaggcg gcgcagcgcc tcgagtcccg accagagctg
     1861 gggccgccgc ccgatgctac gcagccggtc gagctcgccc tgcgcgattc cggcacgtgc
     1921 gcggtcgaac cagccgccct cgagcgcgtc gaggacgtcg agcagccgcc atccgtgtgc
     1981 gccgagccgg agcaggcgca gtacccagac gccgaacgga cgctcggaaa gccgctcgcc
     2041 cgcggcgggg tcgagtggta ggtcgaactc gcggaacact cgtcgggacg cggcgaggtg
     2101 gggcccgacc tggcgcatgg tcaccgcgac gtccgaagga cgtagagtcc gatcgtctgc
     2161 cagttgccgc ttgattgacc gcgccacctc gcgtagctgc gcgtcgtggt cggcggccga
     2221 gaacgcctcg aaccccggct cgacgctggc tgcggctggc agcacctcgc gtcgggcgtc
     2281 ggggaccgct gcttcgagtg ccgccagggt ccaggcggcg cgcgcacccg atgtctcgtc
     2341 gagggcgagc catacctcac tgcggcgggc gagcccggca acgagttcga cctcgcccgg
     2401 ctcaaggaac tgcatactgt ccacgagcac gagtggcgcg agtggatgca cgtcgagcgc
     2461 ctgtgcggcg aggcgggccc tcgcttcggg cggatgccag ccccgctcgc ccagcacctc
     2521 gaggtagccg gcatacagcg cggcgagcgc gagcaggtcg ggctgaccga gccgttcggc
     2581 ggctgcgacc agcacgtcgg ggtccacggc ggcgcggcat agctcgccga cccccgcgcg
     2641 caccatcgcg tgcaggccgg gtgtgtgcgc gatggactcg aaccagcggg cagggccgac
     2701 tcccgctgcc tgccctcgga cc
//
LOCUS       DAORPG010000231         2722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3988_length_2722_cov_2.134983, whole genome shotgun sequence.
ACCESSION   DAORPG010000231 DAORPG010000000
VERSION     DAORPG010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3988_length_2722_cov_2.134983"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLX85_05920"
     CDS             complement(<1..187)
                     /locus_tag="PLX85_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62745.1"
                     /translation="MNLLHVVHYPVFGGPHNQALRLAEPLRRRGWETTVLLPDEPGNA
                     AERLREAAERSRRVGDEE"
     gene            complement(184..1527)
                     /locus_tag="PLX85_05925"
     CDS             complement(184..1527)
                     /locus_tag="PLX85_05925"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRC62746.1"
                     /translation="MKVAVVGQGYVGLTATACLAGSGHTVVGVEADLDRLASLEGGVV
                     PFFEPGLPELVQDGLTSGRIRFAPTLAAAGPVEAIVVAVGTPQLPGGNADLRQIARVL
                     NEVRLLPQLPRLVLVKSTMPPGTSDRLLAETEAGAAFSSIYAYNPEFLSQGQTIRGWT
                     DPTRIVVGLYERRVLPIVRELYRGIEAPWVVSTPTNAEMIKYASNAFLATRISFINEI
                     ANLCDEVGADVDEVARGMQLDTRIGAGFLAAGVGYGGSCFPKDTRALTYLASSRGRRM
                     RLLESVIQVNNGQHELVRDRLLQMVEGVKHPAVAILGLSFKPGTDDLREAPSLSVVPD
                     LVGRGCVVRLWDPAVRDAGKRPEFAGASVSSSLQSAIRDAHAALVLTEWPEVVEADWG
                     HLASLMRRPRAVMDGRNCLDAARVVAAGLKYRGVGRAPSPPRVDGAESATGSDVR"
     gene            complement(1524..>2722)
                     /locus_tag="PLX85_05930"
     CDS             complement(1524..>2722)
                     /locus_tag="PLX85_05930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRC62747.1"
                     /translation="PYVVERFNALQRRGNIDFEAWFNERREADRSWDVLEADWDFSAR
                     YITARSVRGRRLWLPLAELREVRPDVLVSLYASASFAGGIASARAMGVRTAIRVLPTF
                     DSWIRRSRAKEISKHLLFRAVDGAKVPGPAGEALAARYGLPRDRIVHVTQSVDVTRYG
                     MARELSVAEREAERERLGLRACTFIYVGRLWRAKGLDFLLDAFERVRARVPQTTLLMV
                     GDGVDEAFYRERAAHIPGVVFAGFVQPADIASLYAVADVSVFPTLGDPHGLVVEEAMA
                     SGLPVISSDAAGDVRARLPDGKAGYVVPAGDAERLAERMVTLALDPALRRTMSLEASR
                     LVADRGHDVWARDFEGFVERVLSLPRRRTPQAYLAGAVGRLLLTTARQSEPAPNVGAF
                     DQGLLP"
BASE COUNT          451 a          994 c          885 g          392 t
ORIGIN      
        1 tctcctcgtc gccgacgcgt cgggagcgct cggccgcctc tcgcaggcgc tcggcggcgt
       61 tgcccggctc atccggcagg agcaccgtcg tctcccaacc ccgccggcgc aacggctcag
      121 caaggcggag cgcctggtta tgtggccccc cgaacaccgg gtagtggacg acgtggagta
      181 ggttcaacgc acgtccgagc cggtagcgct ctcagcgcca tcgacccgtg gtggcgacgg
      241 tgcccgcccg acgccccgat acttgagacc ggctgcgaca acacgcgcag cgtccaggca
      301 gttgcgcccg tccatcaccg cccgcggccg ccgcatgaga ctggcgaggt gtccccagtc
      361 ggcctcaaca acttcgggcc actcggtgag caccagggca gcatgtgcgt cgcgtattgc
      421 ggactgcagg ctgctgctca ccgaggcgcc ggcgaactcg ggccgcttcc cggcgtctcg
      481 caccgccgga tcccacaggc ggaccacgca cccgcgcccg acgaggtcgg gcacaaccga
      541 caggctcggc gcctcccgta ggtcgtccgt gcccggcttg aaactcaggc cgaggatcgc
      601 gaccgccgga tgtttgacgc cttcgaccat ctgcagaaga cggtcgcgga ccagctcgtg
      661 ctgcccgttg ttgacctgga tcaccgactc gagtaggcgc atccgccggc cccgggagga
      721 tgcgaggtag gtcagcgctc gcgtgtcttt ggggaagcag gatccgccgt agccaacccc
      781 cgcggcgagg aacccggcgc cgatgcgcgt gtcgagctgc atcccccgcg ccacctcatc
      841 gacgtcggcg cccacctcgt cgcaaaggtt cgcaatctcg ttgatgaacg agatccgcgt
      901 cgcgaggaat gcgttgctcg cgtacttgat catctcggcg ttcgtggggg tcgagacgac
      961 ccagggcgcc tcgatcccgc ggtacagctc acgcacgatc ggtagcacac gacgctcgta
     1021 cagcccgacc acgatgcgag tgggatccgt ccagccacgg atcgtctggc cctggctgag
     1081 aaactccggg ttgtaggcgt agatgctgga gaaggcagca ccggcctccg tttccgccag
     1141 caatcggtcg ctggtgcccg gaggcatcgt gcttttgacc agtacgagcc gaggcaactg
     1201 tggcagcagg cggacttcgt tgagtacccg ggcgatctgc cgcaggtcgg cgttgccgcc
     1261 cgggagctga ggcgtaccca ccgcgaccac gatcgcctcg accgggccgg cggcagccag
     1321 agtcggcgcg aagcggatcc gcccgctggt caggccgtct tgcacgagct ccggcagccc
     1381 gggctcgaaa aaggggacaa cgcccccttc caggctcgcc agacggtcga ggtccgcctc
     1441 gacgccaaca accgtgtgac cggatcccgc gaggcaggcg gtggcggtga gtccgacgta
     1501 cccctgcccc acgacggcga ccttcatggc agcagtccct gatcgaatgc gcccacgttc
     1561 ggggctggct cgctctgcct ggcggtcgtg agcagcaagc gccccacagc gccggccaga
     1621 taggcctgtg gcgtccggcg ccgggggagc gacagcaccc gctcgacgaa cccctcgaag
     1681 tcgcgggccc agacgtcatg cccacgatcc gcgacgagac gggatgcttc gaggctcatc
     1741 gtgcgtcgca acgcgggatc gagcgcgagg gtgaccatgc gctccgccag gcgctccgcg
     1801 tctccggcgg gcactacgta gcccgccttc ccgtccggca gccgtgcgcg gacgtccccc
     1861 gcggcatcgc tcgagatgac cggcagcccc gaggccatcg cttcttccac gacaaggcca
     1921 tgcggatccc cgagcgtggg gaacactgat acgtccgcga ctgcatacag gctcgcgatg
     1981 tcggcgggct ggacaaaacc ggcgaacacc acgccgggta tatgcgcggc ccgttcgcga
     2041 tagaacgcct cgtcgacacc atcgccgacc atcagcagcg ttgtctgcgg aacgcgcgcc
     2101 cgcactcgct cgaacgcatc gaggaggaag tcgagcccct tcgcccgcca cagccgcccg
     2161 acgtagatga acgtgcaggc tcgcaggccg agccgctcgc gctccgcctc gcgctcggcc
     2221 accgacagct cgcgggccat gccgtagcgt gtgacatcga ccgactgcgt gacatgaacg
     2281 atccggtcgc gcggcagccc gtacctcgcg gcgagtgcct ctccggccgg ccccgggacc
     2341 tttgccccgt ccaccgcgcg gaatagcaga tgcttcgaga tctcctttgc gcgtgaccgg
     2401 cggatccagc tgtcaaacgt gggcagcacc cggatcgctg ttcgcacgcc catcgcccgc
     2461 gcgctggcga tccccccggc gaacgacgca gaggcgtaca gcgagacgag cacgtccggc
     2521 cgcacctcac gcaactcggc aagcggcagc cacagccggc ggccccgcac cgatcgagca
     2581 gtgatgtacc gggcggagaa atcccagtct gcttcgagca catcccagct gcggtcggcc
     2641 tcgcggcgtt cgttgaacca cgcctcaaaa tcgatgttcc cccgccgctg aagcgcgttg
     2701 aaccgctcaa ccacgtacgg cg
//
LOCUS       DAORPG010000232         2721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_3990_length_2721_cov_3.119280, whole genome shotgun sequence.
ACCESSION   DAORPG010000232 DAORPG010000000
VERSION     DAORPG010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3990_length_2721_cov_3.119280"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..166)
                     /locus_tag="PLX85_05935"
                     /pseudo
     CDS             complement(1..166)
                     /locus_tag="PLX85_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263170.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
     gene            354..482
                     /locus_tag="PLX85_05940"
     CDS             354..482
                     /locus_tag="PLX85_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62748.1"
                     /translation="MKWLFCGGFSSARLDLQRESGEIIAVEGGGLEMGVEVMNMSR"
     gene            549..1688
                     /locus_tag="PLX85_05945"
     CDS             549..1688
                     /locus_tag="PLX85_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRC62749.1"
                     /translation="MSRLRLQPRLLSAILVLISFATAILIVGGRQADATATYGPLITQ
                     ALKYDGTRGGECYLFMQKVVKEVTGRLINGDYRQSYLDAGATEIALSQAQSGDIIQLV
                     DDKNTAATASYAGLHTAFIMDVLGGGKFKVIDSNYLFDGIVRVHDNYSPQDSAARYKN
                     ISVHIWRVPLDKVTPTAAKSGTASATPKADATPAPRDTKLIPGTMAIIAADGDCLRIR
                     DVAGLAGKEVACAPTGAQVKVLDGTENLNGYRWQLIEYNGVKGWAADMYLQPVSGIVT
                     AASVDTSPQPAATPEPVLPAILSGEAPADSGGYSLFVFGGGTYEDLLAAAGCPKDTAS
                     FWATSGDGQFSWYIPAVTVAAVNAAWDGIFDGTIPIGTPLMGRCG"
     gene            complement(1685..2173)
                     /locus_tag="PLX85_05950"
     CDS             complement(1685..2173)
                     /locus_tag="PLX85_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HRC62750.1"
                     /translation="MTRDEIIEAIRAADARLEALAPRIRDAGDRPLASGDWRVRDAMS
                     HVAARGNPVPRTLARLNQATDAAGNPQPVRDIHEINAEQVRDRTHADVDDLVRETRDG
                     HRQALTDLEDVDDDVLERRLVVAFPPGELSVAEFIHLAGPRHEGNHLNDIEAVLGDAA
                     SR"
     gene            2273..>2721
                     /locus_tag="PLX85_05955"
     CDS             2273..>2721
                     /locus_tag="PLX85_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007026572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HRC62751.1"
                     /translation="MRVALLDDYQDVALASTDWSVLGPEYEVVPFHDHLADGDALVER
                     LRDFDVAMAMRERTAFPRAVLERLPRLRLLVTAGMRNAAIDVAAARELGITVCGTGGR
                     GYATAELTWGLILALARHIPEEHAAVLGGRWQTTVGMELRHKTLGIVG"
BASE COUNT          424 a          891 c          954 g          452 t
ORIGIN      
        1 gcggccgcgg gccggcgaat tgccagagga aaccggccag cgatccctgc tcgtcgcgga
       61 tgcgccgcac cgcctgcgca ttgccgatcg cggaggcgat cttggcgcgg ttgcgcacga
      121 tgcccgggtt ggccagcagt ttctcctgcc gcgccggcgt gaatcgcggg cgaccgcggc
      181 cacgaggccg agcgcgatgg tcgcaaaggc gagcacgagg agggggatca ggcgcggcct
      241 tctttcgtct gcccacagat tctacacagt gaattcagca gggccgccag cccccacgcc
      301 atcgcggcgg tctcggcccc ccgctgcggg tgcctgtttc gtttgaaatg gagatgaaat
      361 ggctgttctg cggcggtttt tcatcggctc gactcgatct ccaacgggag tctggcgaaa
      421 tcatcgcagt cgaaggagga gggctggaaa tgggggtgga agttatgaac atgagccgct
      481 aggatgtcca cactgcgtcc ggtctgcggg gtccctggac gcgcgttgtg gggcggagac
      541 agggacgcat gagcagactg cgcctgcagc cgcggctgtt gtcggccatc ctcgtactga
      601 tctcgttcgc gaccgcgatc ctcatcgtgg gaggccgcca ggcggacgcg acggcgacct
      661 acggcccgtt gattacccag gcactcaaat acgacggaac ccgcgggggc gagtgctatc
      721 tcttcatgca gaaggtggtc aaagaggtca ccggacggct aatcaatggt gattaccgcc
      781 agagctacct ggacgcgggc gcgaccgaga ttgccctctc gcaggctcag agtggcgaca
      841 tcatccaact cgtcgatgac aagaacacgg ccgccacggc gagctatgcc ggcctccata
      901 cggcgttcat catggacgtg ctgggcggcg ggaagttcaa agtcatcgat tcaaactacc
      961 tgttcgacgg catcgtgcgc gttcacgaca actattcacc gcaagactcg gcggcccgct
     1021 acaagaacat ctcggtgcac atctggcgcg tccccctcga caaggtgacc cccacggccg
     1081 ccaagtcggg caccgcctcg gcgaccccga aggcggacgc gactcccgcg ccgcgagaca
     1141 cgaagttgat ccccgggacg atggcgatca tcgcggctga cggcgactgc ctccgcatcc
     1201 gtgacgtggc gggcctcgcc ggcaaggagg ttgcctgtgc gccaacgggt gcgcaggtca
     1261 aggtgctcga tggcacggag aacctcaacg gctaccgctg gcaattgatc gagtacaacg
     1321 gagtcaaggg ctgggccgca gacatgtacc tgcagcccgt gtccggcatc gtgacggccg
     1381 cctcggtgga cacgagtccg cagccggcgg ctacgcctga gcccgtgctg ccggcaatcc
     1441 tgagcggcga ggcgcccgcg gattccggtg ggtattccct gttcgtcttc ggcggcggga
     1501 cgtacgagga cctgctggcg gcggctggtt gtccgaagga cacggcgtcc ttctgggcga
     1561 cctccggcga cggccagttc tcgtggtaca tccccgcggt taccgtcgcc gccgtcaacg
     1621 cggcgtggga cggcatcttc gacggcacga tcccgatcgg gacgccgttg atggggcgct
     1681 gcggctagcg cgaggcagcg tcgccgagca ccgcctcgat gtcgttgagg tgattgccct
     1741 cgtggcgcgg cccggcgagg tggatgaact ccgccacgct cagttcgccg ggcgggaacg
     1801 ccacgacgag tcgtcgctcg aggacgtcgt cgtccacgtc ctccagatcc gtgagcgcct
     1861 ggcgatgccc atcgcgggtc tcgcggacca ggtcgtccac gtctgcgtga gtgcggtcgc
     1921 gcacctgttc cgcattgatt tcgtggatgt cgcgcaccgg ttgtgggttg ccggcggcgt
     1981 ccgtcgcctg gttcaggcgc gcgagcgtcc tcggtacggg gttgccgcgc gcggcgacgt
     2041 ggctcatcgc atcgcgcacg cgccagtcac ccgaggcgag cgggcggtcg cctgcgtctc
     2101 ggatacgagg cgccagggcc tccagccgtg cgtccgcggc gcggattgcc tcgatgatct
     2161 cgtcacgtgt caccgtcgcc ccctctgagc tggcccgccg cgcggtcgac gggtgcctgt
     2221 tcgagatcat actgtgcgcg cgaggtgcgg ccgcggacga acggggaagc tcatgcgagt
     2281 ggcgctgctc gacgactacc aggatgtggc gctggcgagc acggactggt ccgtgctggg
     2341 cccggagtac gaggtcgtcc cgttccacga tcacctcgcc gatggggacg cgctcgtcga
     2401 acggttgcgg gacttcgacg tggccatggc gatgcgagag cgcaccgcct tcccgcgagc
     2461 cgtcctcgag cggctgcccc ggttgcgact cctcgttacc gcgggaatgc gcaacgccgc
     2521 gatcgacgtc gcggccgcca gggagctggg catcaccgtc tgcggcaccg gcggccgggg
     2581 ctatgccacg gcggagctca cctggggcct catcctcgcg ctggctcggc atatccccga
     2641 ggagcacgcc gcggtcctcg gagggcggtg gcagacgacc gtcggcatgg agttgcggca
     2701 caagacgctg ggcatcgtcg g
//
LOCUS       DAORPG010000233         2696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4061_length_2696_cov_2.897387, whole genome shotgun sequence.
ACCESSION   DAORPG010000233 DAORPG010000000
VERSION     DAORPG010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4061_length_2696_cov_2.897387"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(264..1031)
                     /locus_tag="PLX85_05960"
     CDS             complement(264..1031)
                     /locus_tag="PLX85_05960"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRC62752.1"
                     /translation="MTSIVSRLVRGPASLALALLVLGAVPLVAVGCGNSSSADVETTP
                     IVGTVPFADGERLTYELRDDRGVLGHGVLTVTREGNEFVLKQDYEEATTPEGATATSD
                     RSTSRVDVATLQPHSVERTVQGRDDTTEYAAEYSDDGSSVRLSRDGGHERDVKLPEYS
                     YENESSLWLWRSLDLAEGYKSKYVSVNLVERTRQTVALEVTGRQRTTVPAGTFDTWRL
                     QIRNGRATRVVWVNVDAPHQVIQWDNGDVVFRLEPNR"
     gene            complement(1069..1515)
                     /locus_tag="PLX85_05965"
     CDS             complement(1069..1515)
                     /locus_tag="PLX85_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62753.1"
                     /translation="MDPDFGIDIEEMGRVFDEAEVVVVRFHVISQRLLIDARSAPGDP
                     PLIRLVPPASSAEERYRYLQRERPGMPLPEQITVVGWPRYIQVMKDVGLWSRIEERLV
                     RESGPAAVQDAQHAFDDVRSAERHEVAAAILGGEGYESLWERSAQR"
     gene            complement(1578..2387)
                     /locus_tag="PLX85_05970"
     CDS             complement(1578..2387)
                     /locus_tag="PLX85_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRC62754.1"
                     /translation="MTATSEAAGGAGRRGWLREGYPRSFIIGTTFAVAWSPCIGPILG
                     AVLTLAATSGTALQGGLLLVAYSLGLGVWFLAFGAFFGWIAPRMRRVQRYMPWVLMVA
                     GALFIVVGAMMFLGEFGQLNQRFQSLGFFFGRTSDAEEQLSSGTSGALGPAVAFVGGV
                     VSFLSPCVLPLVPVYLANLAGVAAIESGAGGGERRRLMLHSAAFVVGFTIVFASVGAS
                     AGLAGDLVQDQLGLLARVGGAVLIVFGLHMSGLIHLPYLDRTYQLPVGSGG"
     gene            complement(2391..2591)
                     /locus_tag="PLX85_05975"
     CDS             complement(2391..2591)
                     /locus_tag="PLX85_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62755.1"
                     /translation="MVSDDVRSEYQAAWADWQAQLAKVHEFFLDGVRPHPTQIKGMLN
                     REARAKERYDEARRRLLGISDE"
BASE COUNT          437 a         1074 c          823 g          362 t
ORIGIN      
        1 gcccgcgccg gctcctcgac cctgctctgc cccaccaccg aacccacgtt cgaggacagc
       61 ggctggctcc tccgcccggc ctgaccgccg gtttcatcca catatggtgc ttcgccatct
      121 cgctcggagc ccctcccctc gatggggtag gagatggcgc gctgggaggc gtcgcgcgcg
      181 cagcgcgcag ccgcgaaccg cgcaaccggc gggactgtat ggatggcgag ccagctcaga
      241 ccctgcgagg acgcccgtcg aacctaccgg ttcggctcca gccggaacac cacgtccccg
      301 ttatcccact ggatcacctg gtgcggcgca tcgacgttca cccagaccac ccgggtcgcg
      361 cggccatttc gaatctgcag ccgccacgtg tcgaacgtcc ccgccggcac cgtcgtccgc
      421 tgccgccccg tcacctcgag ggccaccgtc tgccgggtcc gctccacgag gttcaccgac
      481 acgtacttgc tcttgtaccc ctcagcgagg tccagcgacc gccagagcca gagcgacgac
      541 tcattctcat aggagtactc cggcagcttc acatcgcgct cgtggccgcc gtcgcgtgac
      601 agccgcacgc tgctgccgtc gtccgagtac tccgccgcat actccgtggt gtcgtcgcgc
      661 ccctgcaccg tccgctccac cgagtgcggc tgcagcgtcg ccacgtccac ccgcgaggtc
      721 gaacggtccg aggtcgcggt cgcgccctcg ggcgtcgtcg cctcctcgta gtcttgcttc
      781 agtacgaatt cgttgccctc gcgggtgacc gtcaacacgc cgtggcccag tacgccacgg
      841 tcgtcgcgca gctcgtacgt cagccgctca ccgtccgcga acggcaccgt ccccacaatc
      901 ggcgtggtct ccacatcggc tgaggagctg ttgccgcacc cgaccgcgac cagcggcacc
      961 gcgccgagga ccaacagcgc cagcgccagc gatgccggcc cccgcaccag ccgcgagacg
     1021 atcgaggtca tcggtcgagc gcggcgagga cggtcgaggc tcggttggtt agcgctgggc
     1081 actgcgctcc cacagcgact cgtacccctc gcccccgagg atggccgccg cgacctcgtg
     1141 ccgctccgcg gagcgcacgt catcgaacgc gtgctgtgcg tcctgcacgg ccgccgggcc
     1201 tgactcccgc accaggcgct cctcgatgcg gctccacagg cccacgtcct tcatcacctg
     1261 gatatatcga ggccacccga ccacggtgat ctgctccggt agcggcatcc ccggccgctc
     1321 acgctgcagg tagcggtagc gctcctccgc cgaagacgcc ggcggcacga ggcggatcag
     1381 cggcgggtcg cccggcgccg aacgcgcgtc gatgagcagc cgctgcgaga tcacgtggaa
     1441 gcggaccacc acgacttctg cctcgtcaaa gacgcggccc atctcctcga tgtcgatccc
     1501 gaagtcgggg tccatgcctc aattgtacgg gcggcctgcg ctagggccag catcgaggcg
     1561 aaacaagccc cgagccgtca gcccccgctg ccgaccggca gttggtatgt gcggtcgagg
     1621 tacggcaggt ggatcagccc gctcatgtgc agcccgaaca cgatcagcac cgcgcccccg
     1681 acccgcgcca gcagcccgag ctggtcctgc acgaggtcgc cggccaggcc cgccgacgcc
     1741 ccgacgctcg cgaagacgat cgtgaagccc acaacgaacg ccgccgagtg cagcatcagc
     1801 cgtctccgct cgccgcctcc cgcgcccgac tcgatcgccg cgacccccgc aaggttcgcg
     1861 aggtacacgg gcaccagcgg cagcacacac ggcgagagga acgaaaccac cccgcccacg
     1921 aacgcgaccg ccggccccag tgcccccgaa gtccccgacg agagctgctc ctcggcgtcc
     1981 gaggtgcgcc cgaagaagaa gcccagcgac tgaaaccgct ggttcagctg cccgaactca
     2041 ccgaggaaca tcatcgcccc gaccacgatg aacagtgccc ccgccaccat gagcacccac
     2101 ggcatgtacc gctgcacccg ccgcatccgc ggcgcgatcc agccgaagaa cgcgccgaac
     2161 gccaggaacc acacaccgag gccgagcgag tacgccacga gcagcaatcc gccctgtagc
     2221 gccgtccccg aggtcgcggc cagcgtcagc accgccccga ggatcgggcc gatgcacggc
     2281 gaccacgcca ccgcgaacgt cgtgccgatg atgaacgaac gcgggtaccc ctcgcgcagc
     2341 cagcctcgcc gcccggctcc gcccgccgcc tccgaggtag ccgtcacccg ctactcgtcg
     2401 gagatgccga ggagccgccg gcgcgcctcg tcgtagcgct ccttggcgcg cgcctcgcgg
     2461 ttcagcatgc ccttgatctg cgtcgggtgt ggccgcaccc cgtcgaggaa gaactcgtgc
     2521 accttcgcca gctgggcctg ccagtcggcc cacgccgcct ggtactcgct gcgtacgtcg
     2581 tcgctgacca tgcgttcagt ctagcaacgc tgcgtcgcct gggacacgac ggagcggtat
     2641 ggcgagactc acacgcgcaa cggcggcgcg gcccgcggcc gccaccgcac ccgccc
//
LOCUS       DAORPG010000234         2689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4084_length_2689_cov_3.123766, whole genome shotgun sequence.
ACCESSION   DAORPG010000234 DAORPG010000000
VERSION     DAORPG010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4084_length_2689_cov_3.123766"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1348)
                     /locus_tag="PLX85_05980"
     CDS             complement(155..1348)
                     /locus_tag="PLX85_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005193338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRC62756.1"
                     /translation="MSDEPQRRKPNVYIEDRWFYTRGARKGEKKDRHGVGDRWRVHVV
                     GFDQDRESETFDRKADAEAYRDDAKSRLLQGSYVTDRAGAVTVATVWEEYLSHQKAGQ
                     TKDRRMSAWRTWVKPKWAKRAVRDVSRSGVKAWIVEMGEKGATPTTVENAMEVLRGIL
                     TVAVDDKRLATNVARGLELPARIEPPKAYLSHQQVRELGETIDPRYVTLILFLAYTGL
                     RFGEAAALTVQDVDFLRRQAHVRQQVSESKGGLSWTPTKGKQRRHVPIPRFLMEPLSV
                     QVSGKKRTDYVFTAPRGGVLRLNTWRDRIWNPALDTLCGRDDKGVAHTDFPEATPHDL
                     RHTAASLAVQAGANVKALQAMLGHKKATLTLDTYAELFPEDLEQVAAKLDDAVTALAL
                     GRHGA"
     gene            complement(1341..1913)
                     /locus_tag="PLX85_05985"
     CDS             complement(1341..1913)
                     /locus_tag="PLX85_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC62757.1"
                     /translation="MVQSEDVAIGAAIRRRREAAGMQQQVLAAAVGLAPGVMGRVELG
                     TRACRASELRIIAKELGTTTDELLRDVTPISTAELVERAETRRDIAYKGLREYAEAVA
                     DAMRAVADESVQLGDDELSSPADVREHLSRTQPDYLGVTAEKALVPEIAAALDALRAS
                     LPVKAPRKTPLIDTAGKTFAELQEELNHDE"
BASE COUNT          378 a          970 c          881 g          460 t
ORIGIN      
        1 tagcgcgctt cgttcgggac gaagaggtcg tgggttcgaa tcccgccacc ccgacagtga
       61 gaaagaccct ctgacctgca tcgcagggag gagggtcttt cttcgtctcg gcgggcggag
      121 gttccgtgcg gcagtggtgc ggcagtggaa aaactcaggc cccgtgccgt ccgagcgcca
      181 gcgcggtcac ggcgtcgtcg agtttcgccg ccacctgctc caagtcctcg gggaacagct
      241 cggcgtaggt gtccagggtc agcgtggctt tcttgtggcc gagcatcgcc tggagagcct
      301 tgacgttcgc cccagcctgc acagccaacg acgcggcggt gtgcctcaag tcgtgcgggg
      361 tcgcctcggg gaagtcggtg tgggcgactc ccttgtcgtc gcgcccgcag agagtgtcca
      421 gcgcggggtt ccagatgcgg tcgcgccacg tgttcagccg caagacgcct ccacggggag
      481 cggtgaacac gtagtcggtg cgcttcttcc ccgacacctg cacgctcagc ggctccatca
      541 ggaaccgcgg gatcggaacg tggcggcgct gctttccctt ggtcggcgtc cagctcagcc
      601 cacccttgga ctcggacacc tgctggcgca cgtgcgcctg gcgtcgcagg aaatccacgt
      661 cctgcacggt gagcgccgca gcctcaccga acctcaagcc tgtataggcc aggaacagga
      721 tgagcgtgac atagcgcggg tcgatggtct cgccgagttc gcgcacctgc tggtggctca
      781 ggtacgcctt cggcggttcg atccgggcgg ggagttccag gccgcgcgcc acgttcgtcg
      841 ccagccgctt gtcgtcgacg gcgacggtca ggattccgcg cagcacctcc atcgcgttct
      901 cgacggtggt cggagtcgcg cccttctccc ccatctccac gatccacgcc ttgacgcccg
      961 atcggctcac gtcgcgcacc gcgcgcttcg cccacttcgg cttcacccag gtgcgccacg
     1021 cgctcatccg gcggtctttg gtctggcccg ccttctggtg gctcaggtac tcctcccaca
     1081 cagtcgcgac ggtgacggcg cccgcgcggt cggtgacgta gctgccctgc agcagccgcg
     1141 acttcgcgtc gtcgcggtac gcctcggcgt cggccttgcg gtcgaaggtc tccgactccc
     1201 gatcttggtc gaacccgacg acgtgcacac gccagcggtc gccgacgccg tgccggtcct
     1261 tcttctctcc cttgcgcgca ccccgggtgt agaaccaccg atcctcgata tagacgttcg
     1321 gcttccgccg ctgcggctcg tcactcatcg tggttcagct cctcttgcag ttcggcgaac
     1381 gtcttgcccg ccgtgtcgat cagcggtgtc ttgcgcggcg ccttgacggg gagcgaggca
     1441 cgcagcgcgt cgagcgcggc ggcgatctcg ggcacgaggg ccttctcagc ggtaaccccc
     1501 aggtagtccg gctgcgtccg cgacaggtgc tcgcgcacgt cggcgggact gctcagctcg
     1561 tcgtcgccga gctgcaccga ctcgtcggcc acggcgcgca tcgcgtcggc gaccgcctcg
     1621 gcgtactccc gcagtccctt gtacgcgatg tcgcgtcggg tctcggcgcg ctccaccagc
     1681 tcggcggtgc tgatcggagt cacgtcccgc agcaactcgt cggtcgtggt gcccaactcc
     1741 ttggcgatga tccgcagctc ggaggcgcgg cacgcgcggg ttcccagctc cacccgcccc
     1801 atcaccccgg gagccaggcc gacagccgcg gccaacacct gttgctgcat gcccgccgcc
     1861 tccctccgcc gccggatcgc cgctccgata gccacatcct cgctctgcac catgcgcgtc
     1921 actatacaga atgtgcaatc tatccaatcc gtacagcatc gtgctacagt gatacacgta
     1981 tcgtctgcac tgcgcgtact gccctgcata gcccggcttt tcctgcaccg tgctcggcgg
     2041 cgcgacgctc gtggtcgtga tgctggacaa gttcggcgtc ggcgcgggac gcgaggcaag
     2101 cgcatcgcat ttcccgccga caccgatcag cgcgctcagc gcccaagcgc ccgccgcgcc
     2161 gaggacaagc gcgtgccaga gacgcggcct gcgcaacggg taacccgccg cgtggtcggg
     2221 tgtgccatac ccgtcgtcat cggggtcata cggcgcggtc atgctgcaac ccctccgctg
     2281 ctctgcgggg acttggtgga cggcgcgctc ggcgctgtgc ccgtcggcac gcagagcaga
     2341 ccctgcagca ccttccctgg cgggcactgg aggacggcgc accatgtgaa accggtgcgc
     2401 gagttcttca tcaacgtttc accaggcccg cagctcggcg acgcgctcgc cgtcggggtc
     2461 ggcgccgcac cgaggagtcc ggcggtcaag agcgcggcgg caagcgtcac ggcgcggcgg
     2521 cggttcactt cacgctccgt gcgtcacgga tcgcgtagcc gatgtacgca gcgagcaaga
     2581 gcgccacgat gccgaccgcg aagctcgccg cgaccgcggc gggccacatg gggtcggggg
     2641 tgaccccgcc gagctgcgcg aggtgctcag cgacccgatc cagcccgcc
//
LOCUS       DAORPG010000235         2685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4099_length_2685_cov_4.074905, whole genome shotgun sequence.
ACCESSION   DAORPG010000235 DAORPG010000000
VERSION     DAORPG010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4099_length_2685_cov_4.074905"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLX85_05990"
     CDS             <1..513
                     /locus_tag="PLX85_05990"
                     /inference="COORDINATES: protein motif:HMM:NF018533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HRC62758.1"
                     /translation="ELRYADARRMGRWYLVPVDSLMTVPQFEELGPDALEVDEETFLR
                     RIRRHAGQIKSVLTNQKFIAGIGNAYSDEILWEAQLHPHRRRSTMDEDDLRRLYRAMR
                     STLEWARPILEAEVAEGLYQSNEEWRAHLRVHRKGGESCPRCGNEVKSQVRGGSETNY
                     CLHCQPLVLG"
     gene            576..1280
                     /locus_tag="PLX85_05995"
     CDS             576..1280
                     /locus_tag="PLX85_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62759.1"
                     /translation="MKIIGRDIGDSLDRMYHEVEARLAAAERDLKVVPYEGEGLSNVK
                     WSSKAVTISLHSAAPTHALPHIFGVALQHVRQDLDGYPNVRKPKGRQPEGADLVRSAL
                     RELVLEPDAEAQLTGLGLDREWEQEQRHQAMKDLLRDPPADWNEEGSLGNDFIALQCA
                     RLEVTHPPKMWDALRKRVEEVLPVAAQRSGIASRALRRKRWGSAQACLQSLINVRDDL
                     NLGDIATIADGAGREY"
     gene            complement(1303..1854)
                     /locus_tag="PLX85_06000"
     CDS             complement(1303..1854)
                     /locus_tag="PLX85_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRC62760.1"
                     /translation="MPVSIERRALVLNQNYEPLNVCGARRAFVLVYHGKAELIHAAED
                     DIRGVSSRYPLPSVIRLHQPVRRPRPRVRLTRKEVFARDQQRCQYCGRDGVELTLDHV
                     LPRHRGGPHEWENLVAACRPCNHRKGGRTPHEAHMTLLRQPVRPSATPAALFGHYLER
                     YREWHPFVIGWLDRGSRAVYAAS"
     gene            complement(1916..2449)
                     /locus_tag="PLX85_06005"
     CDS             complement(1916..2449)
                     /locus_tag="PLX85_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRC62761.1"
                     /translation="MHWIDLVILAVIAWTTFAAFRSGLIREVIPLASVILGAILASKF
                     YDNLAADIDFIIDDEATRKFIAFVAIFVGIVVVGHIASVLLRTMATLLMLGPLDHVGG
                     AVFGFLKGLLFVEILVFAATSFPVAGGVERAMNDSAFASFFVERLPLMKSLMPGEVKQ
                     ALNTFKDGHRPGQGADR"
BASE COUNT          461 a          864 c          966 g          394 t
ORIGIN      
        1 gaactccgct acgcggacgc ccgccggatg ggccgctggt acctcgtgcc cgtcgacagc
       61 cttatgaccg tcccgcaatt cgaggagctg ggtcccgacg cgctcgaggt ggacgaggag
      121 acgttcctgc ggcggatccg ccggcacgcg gggcaaatca agagcgtact gacgaaccag
      181 aagttcatcg cgggcattgg gaacgcgtat tccgacgaga tcctgtggga ggcgcagctg
      241 catccgcacc gccgccgcag cacgatggac gaggatgacc tgcgccgtct gtatcgcgcg
      301 atgcgctcga cgctggagtg ggcgcgcccc atcctcgaag cggaggtcgc cgaggggctg
      361 taccagagca acgaggagtg gcgcgctcac ctgcgggtgc atcgcaaagg cggcgagtcc
      421 tgcccgcgct gcggcaacga ggtgaagagc caggtccgcg gcggcagcga gaccaactac
      481 tgtctgcatt gccagccgct ggtacttggt tgaaccgtcg cggctgatac cgatccatcc
      541 gtgagcgtga cgcgtactgc gaaggggagg cagtcatgaa gatcatcggc cgcgacatcg
      601 gcgacagcct ggaccgcatg taccacgagg tcgaggctcg cctcgccgcc gcggaacgcg
      661 acctcaaggt ggtcccctat gagggcgagg gattatcgaa cgtgaagtgg tcctcgaagg
      721 cggtcacgat ctcgctgcac tccgccgccc ccacgcatgc gctgccgcac atcttcggcg
      781 tcgcgctgca gcacgtgcgg caggacctcg atgggtaccc caacgtgcgc aagccgaagg
      841 ggagacagcc tgagggcgcc gacctggtac ggtcggcgct gcgtgagctg gtgctggagc
      901 cggacgccga ggcgcagctc accgggctcg gcctcgaccg ggagtgggag caggagcagc
      961 ggcatcaggc gatgaaggat ctgctgcgcg acccgccggc ggactggaac gaagaaggca
     1021 gcctgggcaa cgacttcatc gcgctccagt gcgcgcgcct ggaggtgacg cacccgccga
     1081 agatgtggga cgcgctccgc aagcgcgtgg aggaagtcct cccggttgcc gcccagcgct
     1141 ccgggatcgc gagccgggcg ttgcgccgca agcgctgggg cagcgcgcag gcctgcctgc
     1201 agtcgctgat caatgtgcgc gacgacctca atctgggcga catcgcgacc atcgcggacg
     1261 gcgccgggcg ggagtactag gagcgagcct cggcgccctg cgtcaggaag cggcgtacac
     1321 ggctcgcgag ccgcggtcga gccacccgat gacgaacggg tgccactcgc ggtaccgctc
     1381 gaggtaatgc ccgaacagcg ccgccggcgt tgcggaaggc cggacgggtt gccgcaggag
     1441 cgtcatgtgc gcctcgtgcg gggtgcgccc gcccttgcgg tggttgcagg gccggcaggc
     1501 ggccacgagg ttctcccact cgtgtggccc gccccggtga cgcggcagga cgtggtcgag
     1561 cgtgagttcc acgccgtcac ggccgcagta ctggcagcgc tgttggtccc gcgcgaacac
     1621 ctctttgcgg gtgaggcgga ctcgaggccg cggacggcgc accggctggt gcaggcggat
     1681 caccgatggc aatgggtagc ggctggaaac gcctcggata tcgtcctcag cggcgtgaat
     1741 gagctcagct ttgccgtggt agacgaggac gaaggcgcgc cgcgcgccgc acacgttgag
     1801 cggctcgtag ttctggttca ggaccagcgc tcgccgctcg attgacactg gcatcgctcc
     1861 tgccgtgcac agccagtcta gcgcattcgt ctcggcctcg tcagatggcc agcgctcacc
     1921 gatccgcccc ctgtcccggg cggtggccgt ccttgaaggt gttgagcgcc tgtttcacct
     1981 cgccgggcat cagggacttc atcaggggca gccgttcgac gaagaacgag gcgaaggctg
     2041 agtcgttcat tgcgcgctcg actccgcccg caacggggaa gctcgtcgcg gcgaagacga
     2101 ggatctcgac gaacagcagt cccttaagga acccgaacac cgcgccgccg acgtggtcca
     2161 gcggcccgag catgagcagg gtcgccatcg tgcgcagcag gaccgatgcg atgtgtccga
     2221 ccacgacgat gccgacgaag atcgcgacga atgcgatgaa cttgcgggtc gcctcgtcgt
     2281 cgatgatgaa gtcgatgtcg gcggccaggt tgtcgtagaa cttgctggcg agaatcgctc
     2341 cgaggatgac ggacgcgagc gggatgacct cgcggatcag gccgctgcgg aaggcggcga
     2401 aggtcgtcca ggcgatgacg gcgaggatga cgaggtcgat ccagtgcatg cggcctccgc
     2461 tcgcgagcct cggtgctgcc gggagtctat cagccacagg cggcggcgac agcggcatgg
     2521 caccgcgccc tgtgcgcgcg catactcgct gacgcggcga cggagatgac gatgcgtcca
     2581 gcgaccagga caggcggggc ccggagcgcg ctggggccgg gcggcgccac catcctgatc
     2641 ctcgggatca cctcgttcct gacagacgtc tcctccgaga tgacg
//
LOCUS       DAORPG010000236         2678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4121_length_2678_cov_2.721693, whole genome shotgun sequence.
ACCESSION   DAORPG010000236 DAORPG010000000
VERSION     DAORPG010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4121_length_2678_cov_2.721693"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PLX85_06010"
     CDS             <1..765
                     /locus_tag="PLX85_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRC62762.1"
                     /translation="PPIAGQAAGHQAPPLPDFSQFGPVDREPLSRFRRTVARNMTTSW
                     EQIPYVTLFQTTDITELEDMRRRYRERAREAGGNLTISVVLLKIVAAALKANPHFNSS
                     LDVETNELILKRYYDIGMAVDTERGLAVPVIRGVDDKNIIELAVEMAGIAERARENKL
                     TLEEMRGATFTISNLGSLGTGHFTPLVNWPEVAILGVGRAEQTPVWTDGQWQPRLQLP
                     LSLSIDHRVIDGADGARFLNWIADAIREPLIVAMEG"
     gene            769..2208
                     /gene="lpdA"
                     /locus_tag="PLX85_06015"
     CDS             769..2208
                     /gene="lpdA"
                     /locus_tag="PLX85_06015"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRC62763.1"
                     /translation="MTTSPVTAPEDRPRVVVLGAGPGGYAAAFHAADLGMEVTLVDDA
                     PQPGGVCLYRGCIPSKALLHVAGLIHESRAATEWGVSFGEPKLDLPKLREWKQGVVDK
                     LTRGLGQIARGRRVTYLQGRGYFEGPNRLRVRSDGEISEVEFDYAIVATGSLSAIPQA
                     LRLDSPRVMDSEAALELEEVPETLLIVGGGYIGLELGTVYSALGSQVTVVEMTNGLLP
                     GADRDLVEPLQRRLKREFRDIFLNTRVSALEEVEGAIRATFMGEGEPDNHDFSRVLIA
                     VGRTPNTQRLGLENTQVRLNERGFIRVDGARRTDEPSILAIGDVTGEPMLAHKATHEG
                     RVAAEAIAGMPVAFEPAAIPAVVFTDPEVAWCGLTEAQALDRGIDVAVSRFPWAASGR
                     ATTMGTREGVTKLVIEPSTERVLGIGVTGHGAGELMGEAVMAIEMGVRVSDLHLAVHA
                     HPTLAETLMEAASLYFGHSPHYLGRQTRG"
     gene            2326..>2678
                     /locus_tag="PLX85_06020"
     CDS             2326..>2678
                     /locus_tag="PLX85_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62764.1"
                     /translation="MRRLMVVTAVLLVLSGCSAKTPTTGDDDSGNLIPRPKTVEQLTQ
                     RTRLIVIGTVLDVQGERSEGPIGGSLPTASGVPSLPTLAFTYYTVRADSIVAGADLKP
                     GERFTLRVNGSSKERT"
BASE COUNT          450 a          927 c          882 g          419 t
ORIGIN      
        1 ccgccgatcg cggggcaagc ggccggccat caggcgccgc cgctgcctga cttctcgcag
       61 ttcggcccgg tcgaccgcga gccgctgtcg cgcttccggc gcacggtggc gcgcaacatg
      121 accacctcgt gggagcagat cccatacgtg acgctcttcc agaccaccga catcaccgag
      181 ctcgaggaca tgcgccggcg ctaccgcgag cgagcacgcg aggcgggcgg caacctcacg
      241 atctcggtcg tgctgctcaa gatcgtggcc gccgccctca aggcgaaccc gcatttcaac
      301 tcgagcctcg acgtcgagac caacgagttg atcctcaagc ggtactacga catcggcatg
      361 gcggtcgata ccgagcgcgg cctcgccgtg ccggtgatcc gcggcgtcga cgacaagaac
      421 atcatcgagc tcgccgtcga gatggcaggc atcgccgagc gcgcccgcga gaacaagctc
      481 acccttgagg agatgcgggg agcgacgttt acgatctcga acctcgggag cctcgggacc
      541 gggcacttca ccccgctcgt gaactggccg gaggttgcca tcctcggcgt gggacgcgca
      601 gagcagactc ccgtgtggac ggacggacag tggcagccga ggctccagct gccgctttcg
      661 ctgtcgatcg accaccgcgt gatcgacggc gcggacggcg cgcgcttcct caactggatc
      721 gccgatgcta tccgcgagcc gctgatcgtc gcgatggaag gctgagccgt gaccacttcc
      781 cccgtgacag cccccgagga tcgaccgcgt gtcgtcgtgc tcggggcggg accgggcggc
      841 tatgccgccg ccttccacgc cgccgacctc gggatggagg tcactctcgt agacgacgcg
      901 ccgcagccgg gcggcgtctg cctgtaccgc ggctgcatcc cctcgaaggc gctgctgcac
      961 gtcgcgggcc tgatccacga gtcacgcgcc gcgaccgaat ggggcgtcag cttcggagag
     1021 ccgaagctcg acctcccgaa actgcgcgag tggaagcagg gcgtggtcga caagctcacg
     1081 cgcgggctcg gtcagatcgc gcggggacgc cgtgtcacct acttgcaggg acgcggctac
     1141 ttcgagggcc ccaaccgtct gcgggtgcgc agcgacggcg agatcagcga ggtcgagttc
     1201 gactacgcga ttgtggcgac aggctccctg agcgcgatcc cgcaggcgtt gcgcctcgac
     1261 tcgccccggg tcatggactc ggaagccgca ctcgagctcg aggaagtgcc ggagacgctg
     1321 ctcatcgtgg gcggcggcta catcggcctc gagctgggca ccgtgtactc cgcgctcggc
     1381 tcgcaggtca ccgtcgtcga gatgacgaac ggattgctgc ctggcgccga ccgcgacctc
     1441 gttgagccgc tgcagcggcg cctgaagcgt gagttccgcg acatcttcct gaacacacgc
     1501 gtctcggccc tcgaggaagt cgagggcgcg atccgcgcga cgttcatggg cgagggcgag
     1561 ccggacaacc acgacttcag ccgggtattg atcgcggtcg ggcggacgcc caacacacag
     1621 cggctcggcc tcgagaacac acaggtgcgg ctgaacgagc gcggcttcat ccgcgtcgac
     1681 ggcgcgcgcc gcaccgatga gccctcgatc ctcgcgatcg gcgacgtcac tggcgagccg
     1741 atgctggccc acaaagcaac gcacgaggga cgcgtggctg ccgaggcgat cgcgggcatg
     1801 cccgtcgcct tcgagcccgc cgccatcccc gccgtcgtct tcaccgaccc cgaggtcgcc
     1861 tggtgcggcc tgacggaggc gcaggcactc gaccgcggca tcgacgtcgc cgtctcgcgc
     1921 ttcccgtggg ccgcctcggg gcgcgccacc acgatgggga cgcgcgaggg tgtgaccaag
     1981 ctcgtgatcg agccgagcac ggagcgcgtc ctcggtatcg gcgtcacggg ccacggtgcc
     2041 ggcgagctga tgggcgaggc cgtcatggcc atcgagatgg gcgttcgcgt gtccgacctg
     2101 cacctcgccg tgcacgcgca cccgacgctc gccgagacgc tcatggaggc agcaagcctc
     2161 tacttcggtc attcgccgca ctacctcggg cggcagacgc gaggttagct caccatccgt
     2221 gccgccctaa gccttccatt ggatccctcc gttgcggtcg cacgacatca gcgacatcaa
     2281 caagttgttc tgatcagcca gggctgccgc tgaggggggt tcgtcatgcg tcgactgatg
     2341 gtcgtaaccg cagttctctt ggtcctttct gggtgctcag cgaagacgcc aaccacagga
     2401 gacgacgact caggcaacct gataccacgt cccaagacgg ttgagcagct gactcagcgc
     2461 acgagactca tcgtgatcgg aacggttctc gatgttcagg gagagcgctc tgagggcccg
     2521 atcggcggct ctctcccaac cgcatctggg gtaccgtctc tgccaacact cgctttcact
     2581 tattacaccg tgcgagccga ctctattgtg gctggcgcgg acctcaaacc cggcgagcgg
     2641 ttcaccctcc gtgtaaacgg ctcttccaag gagagaac
//
LOCUS       DAORPG010000237         2674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4134_length_2674_cov_2.819779, whole genome shotgun sequence.
ACCESSION   DAORPG010000237 DAORPG010000000
VERSION     DAORPG010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4134_length_2674_cov_2.819779"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1761)
                     /locus_tag="PLX85_06025"
     CDS             complement(<1..1761)
                     /locus_tag="PLX85_06025"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRC62765.1"
                     /translation="MTTTESSPREMATAYEPAQVEQRLYEWWEANGFFQPSGPGPDGK
                     RPPFTIIMPPPNVTGELHLGHALTTAIEDILVRWHRMRGDDTLWLPGVDHAAIAVQNV
                     VERELRREGIERRDIGREEFLRHVWTWVHKTRDAIGRQHRRLGASADWTRERFTMDEG
                     PQRAVRQTFVNLYNDGLIYRGERLINWCPDCGTALSDLEVEHADEPGSFWYVRYPVAN
                     ENGEATDDYIVIATTRPETIPADTAVAVHPEDDRYRHLIGRHVVVPTSARAIPVIADE
                     AVSIEGGSGALKVTPGHDPVDFEIGQRHGLPVLTVIGLDARMTDAAGEYAGLDRFEAR
                     ERVVADLEGRGLIEKIEAYTHSIGHCQRSGTIVEPLLSEQWFVRTEPLARPAIEAVRD
                     GRIKFVPQRFDRTYFHWMENIRDWTISRQIWWGHRIPVWYCDDCGTMTLPTAEDPMAD
                     PTTCAGCGSSAIHQDEDTLDTWFSSGLWPHSTLGWPDPDAQDLRRYYPTQVMETGYDI
                     IFFWVARMIMLSLYNMDGVVPFETVYLHGLVRAPDGSKMSKSKGNTVDPLQLIDSYGT
                     DALRYALISSTSPGNDQRITD"
     gene            complement(2206..>2674)
                     /locus_tag="PLX85_06030"
     CDS             complement(2206..>2674)
                     /locus_tag="PLX85_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62766.1"
                     /translation="RASNERRQWLIVVHKTLPVGQEVGYSFRWRRDEARGLNRSPGRR
                     YPHLTIAELSGAFVFASNALKEDSVQLPGEADANRKLLERLETDVHRTDVVCDLSGIT
                     TIATTGRLKYVDVGERRLGAFNARRQDGFAPRKRGDQQVRIEKRLSDPAQGRH"
BASE COUNT          387 a          769 c         1045 g          473 t
ORIGIN      
        1 gtcggtgatg cgctggtcgt tgcccggcga ggtgctcgaa atgagggcgt agcggagggc
       61 gtcggtgccg tagctgtcga tgagctggag cgggtcgacg gtgttgccct tcgacttcga
      121 catcttcgag ccgtcggggg cccgcacgag gccgtgcagg tagaccgtct cgaaggggac
      181 gacgccgtcc atgttgtaga gggacagcat gatcatgcgg gcgacccaga aaaagatgat
      241 gtcgtagccg gtctccatca cctgcgtcgg gtagtagcgg cgcaggtcct gggcgtccgg
      301 gtcgggccag ccgagggtgg agtgcggcca gaggccggac gagaaccagg tgtcgagggt
      361 gtcctcgtcc tggtggatcg ctgaggaacc gcagcccgcg cacgtcgtag ggtcggccat
      421 ggggtcttcg gcggtgggga gcgtcatcgt gccgcagtcg tcgcagtacc agacggggat
      481 gcggtggccc caccagatct ggcggctgat ggtccagtcg cggatgttct ccatccagtg
      541 gaagtaggtg cggtcgaagc gctgggggac gaacttgatg cggccgtcac gcaccgcctc
      601 gatggcgggg cgggcgaggg gctcggtgcg cacgaaccac tgctccgaga gcagcggctc
      661 gacgatggtg ccggagcgct ggcagtggcc gatggagtgg gtgtaggcct cgatcttttc
      721 gatcaggccg cggccttcga ggtcggcgac gacacgctcg cgcgcctcga agcggtcgag
      781 gccggcgtac tcgccggcgg cgtcggtcat gcgggcgtcg aggccgatga cggtaagcac
      841 gggcaggccg tggcgctggc cgatctcgaa gtcgacgggg tcgtggccgg gcgtgacctt
      901 gagggcgccc gaaccgccct cgatgctcac ggcctcgtcg gcgatgaccg ggatggcgcg
      961 ggctgaggtg gggaccacga cgtggcggcc gatgaggtgg cggtagcggt cgtcctctgg
     1021 gtggacggca acggcggtat cggcggggat ggtctcgggg cgggtggtgg cgatgacgat
     1081 gtagtcgtcg gtcgcctcgc cgttctcgtt ggcgaccggg tagcgcacgt accagaagga
     1141 accaggctcg tcggcgtgct cgacctcaag gtcggagagg gcggtgccgc agtcggggca
     1201 ccagttgatg aggcgctcgc cgcggtagat gaggccgtcg ttgtacaggt tgacgaaggt
     1261 ctggcggacg gcgcgctggg ggccctcgtc catcgtgaag cgctcgcggg tccagtcggc
     1321 ggaggcgccg aggcggcggt gctggcggcc gatggcgtcg cgcgttttgt gcacccaggt
     1381 ccagacgtgg cgcaggaact cctcgcggcc gatgtcgcgg cgctcgatgc cctcgcggcg
     1441 tagctcacgt tcgacgacgt tctggacggc gatggcggcg tggtcgacgc cggggagcca
     1501 gagggtgtcg tcgccacgca tgcggtgcca gcggacaagg atgtcctcga tggcggtggt
     1561 gagggcgtgg ccgaggtgca gctcaccggt gacgttgggc ggcggcatga tgatggtgaa
     1621 gggcgggcgc ttgccgtccg ggccgggccc cgagggctgg aagaagccgt tggcctccca
     1681 ccattcgtag aggcgctgct cgacctgcgc gggctcgtag gcggtggcca tctcgcgggg
     1741 gctgctctca gtggtggtca tggtgctgtc cttcgatgcg ggtgcgggcg cctcggcggg
     1801 cgggccgcaa acaaagagcc ccgccggagc ggggcagatg gcggcgtgcg gcctcgagag
     1861 ggccggctag ccgccgccgg gtactactac gaggtgggcg tgcatgtcgc ctcgatggta
     1921 ggtgggaggg gcggggcggg caatggggcg gtggtggtgg ggagttgtta ctggtgttgt
     1981 aggtgggagt ggcagttgtt acttttgtgg agaaaagttt caagtggtcg ggtgagatga
     2041 aacggcggta tatgttcacc tatgcgagcc attgacttta cctcggcgtc gccgggcgat
     2101 ctcatcgccc ttccggccgg cgtcgaagca ttcgtcccgc acccgctgcc gcgcttgctt
     2161 gcgctggaac ccgacctcgt atacctcctc gaccaggctt cgcgttcagt ggcgaccttg
     2221 agcggggtcg gagaggcgct tctcaatccg cacctgctga tcgccccgtt tacgaggcgc
     2281 gaagccgtcc tgtcgtcgcg cattgaaggc acccaggcgt cgctcaccga cgtctacgta
     2341 tttgaggcga ccggtcgtgg cgatcgtagt gatgcccgag aggtcgcaaa ctacgtccgt
     2401 gcgatgaacg tcggtctcga gacgctcgag cagcttccgg tttgcgtccg cctcgccagg
     2461 gagctgcacg gagtcctcct tgagggcgtt cgaggcgaag acaaacgccc cggagagctc
     2521 cgcgatcgtc aggtgtggat agcgcctccc gggactccga ttgagtccgc gcgcttcgtc
     2581 ccgcctccag cggaacgagt agccgacctc ctgaccgact gggagcgttt tgtgaacgac
     2641 gatcagccac tgccgccgct cattcgatgc gcgc
//
LOCUS       DAORPG010000238         2672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4138_length_2672_cov_3.229652, whole genome shotgun sequence.
ACCESSION   DAORPG010000238 DAORPG010000000
VERSION     DAORPG010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4138_length_2672_cov_3.229652"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..636)
                     /locus_tag="PLX85_06035"
                     /pseudo
     CDS             complement(121..636)
                     /locus_tag="PLX85_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085261.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
     gene            complement(676..876)
                     /locus_tag="PLX85_06040"
     CDS             complement(676..876)
                     /locus_tag="PLX85_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010077452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HRC62767.1"
                     /translation="MKWITATALLLVVVGAVNWGLWAIAEVDLVAELFGGNTEAASKV
                     VYALVGAAGLYAIWRFPQLIKG"
     gene            1089..1973
                     /locus_tag="PLX85_06045"
     CDS             1089..1973
                     /locus_tag="PLX85_06045"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRC62768.1"
                     /translation="MEYTVHAAGRATGISPWRIRTWERRYGVPAPRRDAGGRRTYTEA
                     DLVVLRRMAALVDQGLSASHAADAIRNEEDTTAAEAAPPALDPRVDVLIDAAAEFDES
                     TCVEVLRLAAETSWATSLEAVVMPALRQVGLRWERGEASIGVEHFLSLVIQRELLTAI
                     ASLPPAEADAPCVLVACAQDDFHDLGATALWLLLRERGAQVICLGADVPARALVLATL
                     TVRPAVVCLSGVAATSTPMLAEAARALVEARCDARVFVGGPAAGGAASEGIPASRLPD
                     PLPAAADALLAAARGHGH"
     gene            complement(1970..>2672)
                     /locus_tag="PLX85_06050"
     CDS             complement(1970..>2672)
                     /locus_tag="PLX85_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62769.1"
                     /translation="GGALRRGAPPSRYAWGFAAVLQAGLVVWLLGLGLQLDAGGAVAV
                     RLATASQLLLGTAVAAIAVLVGWWRHGSRLAPASRHFIWSLRAIAASLTLTGVLLALS
                     AARALIEDVEVTNAQLDAIRHVFLIGVITLAIVVMGQLILPEFASERLVRQPAQWRGR
                     VFAVTLPLAAVFRGVLPLAGLSGEARYWSMSIGGLLGLASVIAFAVLYWRARQSHRTY
                     LARVAGWRGREVPLA"
BASE COUNT          402 a          906 c          961 g          403 t
ORIGIN      
        1 aaagacccgg gccggcgccc gcgccgtcgt cgacgcgctg ctcgcagccg aaaagccggc
       61 cgatttcgcc cctgcggtca gtgcggcggc agccgtcgcg ctgggggtag ccgacgccgg
      121 cgggatgagc accttgtcaa tcacgtggat gacgccgttg ctcgcgcgga cgtccgccgt
      181 gacgaccttc gctccgccca cggtcaccgt gccgccggcg gcgacgatcg gcaggctgcc
      241 gccctggacc gtcttcgcct cggtgagctt cgcggcgtca gccgccagca cctgccccga
      301 caccacgtgg tagagcaaga tgtccttgag cttcgcgggg tccttgagca ggctgtccac
      361 ggtgccggcc ggcagggccg cgaacgcggc gtccgtcggc gcgaagaccg tgaacgggcc
      421 gggccccttt agcgtcgagg tcaggcccgc cgcctcgacg gccgcgagca gggttttgaa
      481 gttcccgttg gcggcagctg tgtcgacgat gttcgcggtc gcctgctcgg gtgcgcggct
      541 ctcgccagcg gagccggtga cgccggcggc gctcaccgag gccatgacgg ccagggcggc
      601 ggcgaggccg gcgatcggtg caatcagctt cttcatgcga tcagtccctc ctgatgatgc
      661 gtcggcggtt tgtgactagc ccttgatgag ctgcgggaag cgccagatgg cgtacaggcc
      721 ggctgcgcca acgagggcgt aaacgacctt gcttgccgcc tcggtgttgc cgccgaagag
      781 ctcggccacg aggtcgactt cagcgatggc ccagaggccc cagttgaccg cgcccaccac
      841 tacgagcagc agggcggttg ctgtaatcca cttcatcgaa tgctccctat cgacatcgcg
      901 ggcttcatga cctcgcgacg ggtatgcctg tcgggttggt tgtgctcgaa ctcggtggtc
      961 tcctttcttt tgccgtttat ttgtcatcga tgctcgttta gttggtcacc gtggtcttat
     1021 ctgaccaaac acggaccggc ctgtcaagtg tcttggcaaa cttttgcaca aatggcacac
     1081 tgtcgcgcat ggaatacacc gtgcacgctg ccggccgcgc gaccggcatc tccccgtggc
     1141 gcatccgtac ctgggaacgg cgttatggcg ttccggctcc gcggcgagac gcggggggcc
     1201 gccggaccta taccgaggcc gacctcgtgg tgctccgccg catggcggcg ctagtcgacc
     1261 agggcctatc ggcgtcgcat gcggccgacg cgatccgaaa cgaggaggac accaccgccg
     1321 ccgaggcagc gccgcccgca ctcgacccgc gggttgacgt actcatcgac gcggcggcgg
     1381 agttcgatga atcgacgtgc gttgaggtgc tgcggctggc ggctgagacg agctgggcga
     1441 cgagcctcga agcggtggtg atgccggcct tgcgccaggt ggggctgcgg tgggaacgcg
     1501 gggaggcctc gatcggggtg gagcatttcc tctccctggt catccagcgc gagctgctga
     1561 ccgcgatcgc cagccttccc ccagcggaag cggatgcacc ctgcgtgctc gtcgcgtgcg
     1621 cgcaggacga tttccacgac ctcggcgcga ctgccctctg gctgctctta cgcgagcgcg
     1681 gcgctcaggt catttgcctg ggggccgatg tgccagcccg cgcgctggtg ctggcgacac
     1741 tgaccgtgcg gccggcggtg gtatgtctga gcggagtcgc ggccacctcg acgccgatgc
     1801 tggcggaagc ggcgcgcgcg ctggtggagg cgcgatgtga cgcgcgcgtc ttcgtcggcg
     1861 gacccgcggc aggtggcgcg gcctccgagg ggatccccgc atcgaggttg ccagacccac
     1921 ttcccgcagc cgcggacgca ctgctcgcgg cagccagggg ccacgggcac taggcgagcg
     1981 gcacctcgcg gccacgccag ccggcaacgc gcgcgaggta cgtgcggtgg ctctggcggg
     2041 cgcgccagta cagcacggca aacgcgatca cggaggcgag cccgagcagg ccgccgatgc
     2101 tcatcgacca gtagcgcgcc tcgccgctca gtccggcgag cgggagcacg cctcggaaga
     2161 cagcggcgag ggggagcgtg accgcgaaga cgcggccacg ccattgagcc ggctggcgca
     2221 ccagccgttc gctggcgaac tccgggagga tcagctgacc catgaccacg attgccagtg
     2281 tgatcacgcc gatgaggaag acgtggcgga tagcgtcgag ttgcgcgttc gtgacctcga
     2341 cgtcctcgat gagggcgcgc gcggcgctca gcgcgaggag gacgcccgtc agcgtgagtg
     2401 aggccgcaat cgcgcgcagc gaccagatga aatgcctcga ggccggcgcg aggcggctgc
     2461 cgtgccgcca ccacccgacg agcaccgcga tggcagcaac ggcggtcccg aggagtagct
     2521 ggctggcggt cgcgagccgg accgcgactg cgccgcccgc gtcgagctgc aggccgaggc
     2581 ccaggagcca cacgacgagg cccgcctgca ggacggcagc gaatccccac gcgtaccgcg
     2641 acgggggcgc accacggcga agagcgccgc cc
//
LOCUS       DAORPG010000239         2664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4163_length_2664_cov_3.666539, whole genome shotgun sequence.
ACCESSION   DAORPG010000239 DAORPG010000000
VERSION     DAORPG010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4163_length_2664_cov_3.666539"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="PLX85_06055"
     CDS             complement(<1..1027)
                     /locus_tag="PLX85_06055"
                     /inference="COORDINATES: protein motif:HMM:NF017669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRC62770.1"
                     /translation="MERTAGELYLGQVLDASGQRTDEAMTYDMTDLTTHGVIVGMTGS
                     GKTGLGIVAIEELLTAGVPTLVIDPKGDMGNLALTFPGLSGADFRPWVNEGDAQRAGQ
                     SVDEFAAAQAEQWRSGLERSGLGPADIEQLRASGRVTIYTPGSESGAPLNVIGNMAAP
                     PEGSGAESVREEAAAIATGLLNLVGIEADPLASREHILLSNLIEGAWASGDQLDLATL
                     IARTLEPPVRKLGVFEIDTFFPAADRRALALRLNALVASASFAAWTAGEPLDVGQLLR
                     DPDGRPRVSILYLAHLSDAERQFVVSLVLTKVITWMRAQSGTTDLRALVYMDELFGFA
                     PPTAEPPA"
     gene            1156..2061
                     /locus_tag="PLX85_06060"
     CDS             1156..2061
                     /locus_tag="PLX85_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HRC62771.1"
                     /translation="MRFDDDAQLDTSQIEDRRGRRLAGRGGIAAGGAGGLVAIIIGIV
                     VALSGGGGGNGSLALSPELEQLLNQTTGGADVPSTLAQDCRTGADANAREDCRIVAVV
                     NSVQAWWQSEFASAGQRYTPSQTQFFVGQTVTGCGSATSSVGPFYCPPDQKVYIDLGF
                     FDELRSRFGATGGPFAQAYVIAHEYGHHVQDLTGDLDRIGNDRSGPTSAAVRSELQAD
                     CLAGVWAHNAVGTGFITQLTPDAIADALDAAAAVGDDRIQASIQGQVNPESWTHGSAE
                     QRQRWFQRGYDQGKRASCDTFSGSI"
     gene            2166..>2664
                     /locus_tag="PLX85_06065"
     CDS             2166..>2664
                     /locus_tag="PLX85_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62772.1"
                     /translation="MLAIREMRRRKLQFALVTGVVALVSFLIIMVTGLGLGLYQRAGT
                     ALLSLNGDQLAYADNANLSLIRSRLTEAQVRDIEAKSGADDATPLGYIAAVIEYAPGR
                     RDTAAVVGLVPGSFAEPRTIEGRALSSPDQILVDRSWARLAGTRVGDVLPLPVGFDRR
                     NFTVAG"
BASE COUNT          406 a          911 c          906 g          441 t
ORIGIN      
        1 tcgcgggcgg ctcggccgtc ggcggagcga agccaaacag ctcgtccatg tacacgagcg
       61 cgcgcaggtc ggtcgtcccc gactgtgccc gcatccacgt aatcaccttg gtcagcacca
      121 gcgacaccac gaattgccgc tctgcgtcgg acaggtgcgc gaggtacagg atcgataccc
      181 gtggccgtcc gtccgggtcg cggagcaact gcccgacgtc gagcggctcg cctgccgtcc
      241 acgctgcgaa gcttgcggat gcgacgagcg cgttgagccg cagtgcgagg gcgcgccggt
      301 cagccgcggg gaagaaggtg tcgatctcaa ataccccaag cttgcgtacc gggggctcca
      361 gcgtccgcgc gatcagggtc gcgaggtcga gctgatcgcc gctcgcccag gccccctcaa
      421 tcaagttcga gagcaagatg tgttcgcggc tcgccagcgg gtccgcctcg atccccacga
      481 gattgagcag gccggtcgcg atcgccgccg cctcttcgcg cacggactcc gcgcccgagc
      541 cctccggggg ggcggccatg ttgccgatca cgttgagcgg cgcgccggac tcggagccgg
      601 gggtgtagat cgtgacgcgg cccgaggcac gtaactgctc gatgtccgca ggcccgagcc
      661 cggagcgctc gagaccgctc cgccactgtt ccgcctgcgc ggcggcgaac tcatcgaccg
      721 attgaccggc ccgctgagcg tcgccctcat tcacccaggg gcggaagtcg gcgccactca
      781 gccccgggaa ggtcagcgcg aggttcccca tgtccccctt ggggtcgatc accagggtag
      841 gcacgccggc tgtcagcagt tcctcaatcg ccacgatgcc gaggccggtc ttgcccgacc
      901 ctgtcatgcc gacgatcacg ccgtgcgtgg tgaggtcggt catgtcgtac gtcatcgcct
      961 cgtccgtgcg ctgcccggag gcatcgagca cctgcccgag gtagagttcg cctgctgttc
     1021 gctccacgag gcctccttcg ctcgccgcac cgccgctgcg ccgcactccg gccgatctta
     1081 gccgcggcgc cgggacaaat cgattcgttc caggccgtat gatcctgctc gagtagggcc
     1141 gagggaggtt ggttcgtgcg ctttgacgat gacgctcagc tcgacacgtc acagatcgag
     1201 gaccgacgag ggcgccggtt ggccggtcgt ggcggcatcg ctgcgggggg tgcgggcggg
     1261 ctcgtagcca tcatcattgg catcgtcgtc gccctctcgg gcggtggggg cggcaacggc
     1321 tcgctcgcgt tgagtcctga gcttgaacag ctgctcaacc agaccaccgg cggcgctgac
     1381 gtcccctcaa cgctcgccca ggattgccgc acgggcgcgg acgccaacgc gcgcgaggac
     1441 tgccgcatcg tggcggtcgt gaacagtgtg caggcgtggt ggcagagcga gttcgcgtcc
     1501 gccggccagc gatacacccc ctcgcagacg cagttcttcg ttgggcagac ggtcaccggg
     1561 tgcggctcgg ccacctcgtc cgtcgggccg ttctactgcc cgccagacca gaaggtctac
     1621 atcgacctcg ggttcttcga tgagctgcgc agccgctttg gcgccacggg cggcccgttc
     1681 gcgcaggcct acgtaatcgc gcatgagtac gggcaccacg tgcaggatct gacgggcgac
     1741 ctcgaccgta tcgggaacga tcgctccggg cccacgagcg cggcggtccg cagcgagctt
     1801 caggcggatt gcctggccgg agtctgggcg cacaacgccg tcggtaccgg ttttatcacg
     1861 cagttgacac cggatgcgat tgcggacgcc ctcgacgctg ccgccgcggt gggggacgac
     1921 cgcattcagg cctcgattca gggccaggtc aaccccgagt cctggacgca cggttccgcc
     1981 gagcagcggc agcgctggtt ccagcgcggc tacgaccagg gtaagcgcgc gagctgcgac
     2041 accttcagcg gcagtatctg acggcgattc ggtatacctg aacgtggatt cctgattcgc
     2101 cacctgttcc gtcgcatggc aacctgaatc gaggactgcg gagactcgga aggaacacgg
     2161 cgagggtgct ggcgattcgg gagatgcggc gccggaaact ccagttcgcg ctggtgaccg
     2221 gcgtggtggc tctggtgtcg ttcctgatca ttatggtcac ggggctcggc cttggcctct
     2281 accagcgcgc cggtacggcc ctgctgtcgc tcaacggcga ccagctcgcc tatgcggaca
     2341 acgcgaacct ctccctcatc cgctcacggc ttacagaagc gcaggtgcgc gacatcgagg
     2401 cgaagtcggg ggccgacgac gcgacgccgc ttggctacat cgcggccgtc atcgagtacg
     2461 cgcccgggcg acgagatacg gcggctgtcg tcggcctggt tccgggatcc tttgccgagc
     2521 cacggaccat cgagggacgc gcgctctcct cgccggatca aatcctggtc gaccgctcgt
     2581 gggcgcgcct cgctggcacc agggtcggtg acgtcctgcc gctgccggtc ggcttcgatc
     2641 gacgcaactt cacggtggcc ggca
//
LOCUS       DAORPG010000240         2660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4173_length_2660_cov_3.645681, whole genome shotgun sequence.
ACCESSION   DAORPG010000240 DAORPG010000000
VERSION     DAORPG010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4173_length_2660_cov_3.645681"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="PLX85_06070"
     CDS             <1..1239
                     /locus_tag="PLX85_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRC62773.1"
                     /translation="RRALPGVILMAAFVAVMFLGITHLAIETGAQASHSETVISQIGR
                     TVFTPHSPFYIAVIFGTAAILIMAANTAFADFPRLAAFQAGDGFLPRWLLDRDNRLVF
                     GVGISVLSFAAGLLIIVYRAEVSSLIPLYAIGVFLSFTLSQAGMIVHWQRVRHVPPGQ
                     RIPRYAAGNMLVTTLEYDPRWPWKMAICTIGTIMAAVVTVIFAVAKFTEGAWIILVVI
                     PTLVFLLSRIHAHYQNVEREIGAEEVDIKTYLQRPVRTLQILVVGDLNKHTLPALREF
                     VHMSGYGGIRQAVHVNVDEEATTELKRQWRDLGIDAMGVQLVVLPSHFGAGDVIGTLS
                     SYVHGALSIDPEIRVNVVITDWATSTRWWGWLLTPALHHLTGARLRLAFLAEDRVTVT
                     NYRYLAGSSLALFGDLDRTA"
     gene            1303..>2660
                     /locus_tag="PLX85_06075"
     CDS             1303..>2660
                     /locus_tag="PLX85_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC62774.1"
                     /translation="MRYLLRLLPYTRRYTRRVLFTWLCVFASGAFVMISPVLIREAIN
                     QGLRPVREGRDIVGLNGNEQLLIFAALAIVAFAIARGLTAFGQTYLGETVGQHVAYDI
                     RNEIYNNIQRLSYAYHDRVETGQIMSRATQDVENIRMFFGMSLFRLGYVAVLVIVAVV
                     GMFVINPTLALVSIVTVPVIAWRSIVMSRRMRPMWLEIQQSQAEMTQTAEESLTGIRV
                     VKAFSREPFESEKFRRTVQRQADLNYNQAREMAKHQPLMQGLSMLQIAITVGVGALLI
                     ARGQLQEGDLLAFVLWLNLLQLPIRTVGFLLNGVARCISSAERVFELIDAQSAVTESP
                     GATELRAVEGAVRFEGVSFGYDQISPVLSGIDIEAHPGQVIALLGPAGSGKSTVVNLI
                     PRFYDVTAGRITIDGQDVRDVTIESLRRNIGIVQQDVFLFIGTIRDNIAYGRPGASQE
                     EI"
BASE COUNT          419 a         1025 c          736 g          480 t
ORIGIN      
        1 cgccgcgcac ttccaggcgt gatcctcatg gccgccttcg tcgcggtcat gttcctcggc
       61 atcacgcacc tcgcgattga gacaggcgca caggccagcc actcggagac ggtcatctcg
      121 cagatcggcc gcacggtctt tactccgcac tcgccgttct acatcgccgt catcttcggc
      181 actgccgcga tcctgatcat ggccgccaac acggccttcg ccgacttccc ccgcctcgcg
      241 gccttccagg ccggcgacgg cttcctcccg cgctggctgc tcgatcgcga caaccgcctc
      301 gtcttcggcg tcgggatcag tgtcctctcc ttcgcggcgg ggctgctcat catcgtctac
      361 cgtgccgagg tctccagcct gatcccgctc tacgcgatcg gcgtgttcct ctccttcacc
      421 ctcagccagg ccggcatgat cgtccactgg caacgggtcc gccacgtccc gcccggccag
      481 cgcatcccgc gctacgcggc gggcaacatg ctcgtgacca cccttgaata cgacccgcgc
      541 tggccctgga agatggccat ctgcacgatc ggcaccatca tggccgccgt cgtgaccgtc
      601 atcttcgccg tcgcgaagtt caccgagggc gcatggatca tcctggtcgt catcccgacg
      661 ctcgtgttcc tgctctcacg catccacgcg cattaccaga acgtcgagcg cgagatcggc
      721 gccgaggagg tcgacatcaa gacctacctc cagcgcccgg tccgcaccct tcagatcctc
      781 gtggtcggcg acctcaacaa gcacaccctg cccgcgctcc gcgagttcgt gcatatgtcc
      841 ggctacggcg gcatccgcca ggccgtccac gtcaacgtcg acgaggaggc caccaccgag
      901 ctgaagcggc agtggcgcga tctcggcatc gacgccatgg gcgtgcagct cgtcgtcctc
      961 ccgagccact tcggtgcggg cgacgtcatc ggcacgctca gctcgtacgt ccacggcgcg
     1021 ctctccatcg accccgagat ccgcgtgaac gtcgtgatta ccgactgggc caccagcacc
     1081 cgctggtggg gctggctgct caccccggcg ctgcatcacc tcaccggcgc gcgcctccgg
     1141 ctcgccttcc tcgccgagga ccgagtcacc gtcaccaatt accgttacct cgcagggtcg
     1201 tcgctcgccc tcttcggaga tctcgaccgc acggcatgat gcttaagcgc tcctttactt
     1261 tcgatccgcg ataggagtac ggtagagcgc agggggacct ctgtgcgtta cctgctccgc
     1321 ctcctgccct acacgcggcg ttacacccgt cgggtgctgt tcacctggct ctgcgtcttc
     1381 gcgtccggcg ccttcgtcat gatcagcccg gtgctcatcc gcgaggcgat caaccagggc
     1441 ctccgtcccg tccgcgaggg ccgcgacatc gtcgggctca acggcaacga gcagctcctg
     1501 atcttcgccg ccctcgccat cgtcgccttc gccatcgctc gagggctgac cgccttcggc
     1561 cagacctacc tcggcgaaac cgtcggtcag catgtcgcct atgacatccg caacgagatc
     1621 tacaacaaca tccagcgcct gagttatgcc taccacgacc gcgtcgagac cggtcagatc
     1681 atgtcgaggg ctacccagga cgtcgagaac atccgcatgt tcttcggcat gagcctgttc
     1741 cgcctcggct acgtcgccgt cctcgtcatc gtcgcggtcg tcggcatgtt cgtgatcaat
     1801 cccaccctcg cgctggtcag catcgtcacc gtccccgtca tcgcctggcg ctccatcgtg
     1861 atgagccgcc gcatgcgccc gatgtggctc gagatccagc agagccaggc cgagatgacc
     1921 cagaccgccg aggagtccct caccggcatc cgcgtcgtca aggccttcag ccgcgaaccc
     1981 ttcgagagcg aaaagttccg ccgcaccgtc cagcgccagg ccgatctcaa ctacaaccag
     2041 gcccgcgaga tggcgaaaca ccagccactc atgcagggcc tttcgatgct ccagatcgcg
     2101 atcaccgtcg gcgtcggcgc gttactgatc gcccgcggcc agctccagga aggcgacctg
     2161 ctcgccttcg tcctctggct gaacctgctc cagctcccca tccgtacggt cggcttcctg
     2221 ctcaacggcg tcgcccgctg catcagctcc gccgagcgcg tcttcgagct gatcgacgcc
     2281 cagtccgccg tcaccgagtc accgggcgcc accgaactgc gcgccgtcga gggcgccgtc
     2341 cgcttcgagg gcgtcagctt cggctacgat cagatcagcc ccgtcctcag cggcatcgac
     2401 atcgaggcgc atccgggcca ggtcattgcc ctgctcggtc cggccggcag cgggaagtcc
     2461 accgtcgtca acctcatccc tcgcttctac gacgtcaccg ccggccgcat caccatcgac
     2521 ggccaggacg tccgcgacgt gaccatcgag tccctccgtc gcaacatcgg catcgtccag
     2581 caggatgtct tcctcttcat cggcaccatc cgcgacaaca tcgcttacgg ccgcccgggc
     2641 gcctcgcagg aggagatcga
//
LOCUS       DAORPG010000241         2652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4194_length_2652_cov_3.250674, whole genome shotgun sequence.
ACCESSION   DAORPG010000241 DAORPG010000000
VERSION     DAORPG010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4194_length_2652_cov_3.250674"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1239)
                     /locus_tag="PLX85_06080"
     CDS             complement(136..1239)
                     /locus_tag="PLX85_06080"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRC62775.1"
                     /translation="MAAAIRVGIVGATVTPGGSGWGANAHVPALHALPDFELKAVCTA
                     HPETAEASARAFGAALAFHDIEQMAAHPEVDLVVVSVRVPWHRDLVMAGLSAGKAVFC
                     EWPLGANLVEAEEMAGYARDRGLRTAVGLQGRSDPAVRYAADLVARGDLGEILAANLS
                     VMTQAAWERGSGRVWQRLRTNGANTLTIAGGHGIDAMCAILGEFAEVSARTATRVTEW
                     LDTDAGELVPVDAPDSIGLTGVLTNGIEVTVHVAAVPRPSGSRLEIYGRNGTLTLVSS
                     GALSTGPNQIYAALGDRELAPVPVPSEYTLVPEGTPSGSPRNVAQAYARLAEAFTAGA
                     PFMPDFDHALTRHRLIDAIERSAATGQAIQLLR"
     gene            1410..2315
                     /locus_tag="PLX85_06085"
     CDS             1410..2315
                     /locus_tag="PLX85_06085"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HRC62776.1"
                     /translation="MAAEHLRWRAARDGLNDPIMIAAFMRKNGFGTTALASLTHFATA
                     HDGELLAEIEPEDFFDFTVASPMLERRDGERVLVWPQNRILRLRTGEGARDVLVMLGT
                     EPHLHWGGFAEALQQFITEAGVRELVLASSWPGALPHTRPAFLRLTTEAAELAERLGR
                     SPAPLDYVGPVDFGTTLLMRLDPAVRHARVSAIVPNYLGVVPNPFAMVALIEACDRLC
                     ETQTDIGEIRQLAEEVKAKADEGVRDSSELADALRQMEAQYDAMVAQAGGSAGVEEDD
                     DSLPSPDELLRDVEAFLNQERDGSP"
     gene            complement(2472..>2652)
                     /locus_tag="PLX85_06090"
     CDS             complement(2472..>2652)
                     /locus_tag="PLX85_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62777.1"
                     /translation="LNLFALACVWANISVNTGRSAFWGFATIVPGLNFVLYWVIAWRA
                     LPRREPAGEALPANP"
BASE COUNT          407 a          866 c          968 g          411 t
ORIGIN      
        1 cgggttcaga tctcgtctcc cgctccagat tcacgcctcc ctgttcgctt cacgcgtggc
       61 gattggcaag gtcgaccgga cggcagttcg ctcggtcgtc gtgtgtcggg ggggagcccg
      121 aggctccgcc ctcgatcagc ggaggagctg gatcgcctgg ccggtggcgg cggaacgctc
      181 gatggcgtcg atgaggcgat ggcgtgtcag ggcgtgatcg aagtccggca tgaacggggc
      241 gccggccgtg aacgcctcgg cgaggcgagc gtatgcctgc gccacgttgc gaggcgagcc
      301 ggagggggtg ccctccggga cgagggtgta ctcgctcggc acggggaccg gggcgagttc
      361 acgatccccg agcgcggcgt agatctggtt ggggccggtc gacagcgccc cactcgagac
      421 gagggtgagc gtcccgttgc ggccgtagat ctcgaggcgg ctcccgctgg ggcgggggac
      481 ggcggccacg tgcacggtta cctcgatgcc gttcgtcagc acgccggtga ggccgatgct
      541 gtccggggcg tcgacgggga ccagttcgcc cgcatcggtg tccagccatt cggtcaccct
      601 ggtggcggtg cgagcgctga cctcggcgaa ctcgccgagg atggcgcaca tggcgtcgat
      661 gccgtgtcca ccggcgatcg tgagggtgtt ggccccgttc gtccggaggc gctgccagac
      721 gcggccactc cctcgctccc atgccgcctg cgtcatgacg ctgagattgg cggccagaat
      781 ctcaccgaga tcgccccgcg ccaccaggtc ggcggcatag cgcacggcgg gatcgctgcg
      841 gccctgcagc ccgaccgcgg tccgcagtcc gcgatcgcga gcgtagcccg ccatctcttc
      901 cgcctcgacg aggttggcgc ccagcggcca ttcgcagaag accgctttgc cggcgctcag
      961 gccagccatg acgagatcgc ggtgccaggg gacgcggacc gagacgacca cgaggtcgac
     1021 ctcggggtgc gccgccatct gctcgatgtc gtggaaggcg agagccgccc cgaaggcgcg
     1081 cgccgaggcc tcggcggtct cggggtgggc agtacatacg gccttcagct cgaagtcggg
     1141 cagggcatgg agcgcgggga cgtgggcgtt ggcaccccag ccgctgccgc ccggcgtgac
     1201 cgtggccccg actatgccga ctcggatcgc tgctgccatg gcacctgtgg tcccgtctct
     1261 tgaactcgac gtgcgccgca aggggcgccc ccgtgaattc ccagcagcat acgcgggttt
     1321 ccgtcacggg cgcccgcacg gtgctatggc gacctcgagg tgccgggtca cgtggcggcg
     1381 gcgtggccgt tgcgcgatga tgggcagcga tggcagccga acaccttcgc tggcgcgcag
     1441 cacgggacgg tctcaacgac ccgatcatga tcgctgcgtt catgcgcaag aacgggttcg
     1501 gaacgacggc cctggcgtcg ttgacgcatt tcgcgacggc gcatgacggc gaactcctcg
     1561 cggagatcga gccggaggac ttcttcgact tcaccgtggc gagtccgatg ctcgagcgcc
     1621 gagacggcga gcgggtactc gtctggcctc agaaccgcat tcttcggctg cggacgggcg
     1681 agggggcgcg cgacgtcctc gtcatgctgg ggacggaacc gcatctgcac tggggtggct
     1741 tcgccgaagc gttgcagcag ttcatcaccg aggccggtgt ccgtgagctt gtgctggcct
     1801 cgtcgtggcc cggagcgctg ccacacaccc gcccggcgtt cctgcgcctg acgaccgagg
     1861 ccgccgagct cgcggagcga ctcggtcgct cgccggcacc gctcgactac gtggggccgg
     1921 tggacttcgg cacaacgctg ctgatgcggc tcgatcccgc ggtgaggcat gcacgggtgt
     1981 ccgcgatcgt cccgaactac ctcggcgtag tgccaaatcc gttcgcgatg gtggcgctca
     2041 tcgaggcatg cgaccgtctg tgcgagacgc agaccgacat cggtgagatc cgtcagctcg
     2101 ccgaagaagt gaaggcgaag gcagacgagg gggtcaggga ctcctcggaa ctcgcggacg
     2161 cgctgcggca gatggaggcg cagtacgacg cgatggtggc acaggcagga ggctcggcgg
     2221 gcgtcgagga ggacgatgac tcgctgccgt caccggacga gctgcttcgc gacgtagagg
     2281 cgttcttgaa ccaggagcga gacgggtcgc cttagctcca gaggcgccgg ccccctctcg
     2341 ttcccgtcag cggcgattcg gccactaggc cgagcaagaa gtcagctcac cccgcgaggg
     2401 ggcgtcgcag cagtcggtgc cgatccgctt gagtcggtgg actcggcgcc gggccgaccg
     2461 tgctcgagat cctacggatt cgccggtaac gcttcgccag ctggctcgcg ccgcggcaga
     2521 gcccgccacg caattaccca gtagaggacg aagttcaggc ccgggacgat ggtcgcaaag
     2581 ccccagaatg cagaccggcc ggtgttcacg gagatgttcg cccagacaca cgcgagagcg
     2641 aacaggttga ga
//
LOCUS       DAORPG010000242         2647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4203_length_2647_cov_4.206404, whole genome shotgun sequence.
ACCESSION   DAORPG010000242 DAORPG010000000
VERSION     DAORPG010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4203_length_2647_cov_4.206404"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /gene="pgl"
                     /locus_tag="PLX85_06095"
     CDS             complement(<1..607)
                     /gene="pgl"
                     /locus_tag="PLX85_06095"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HRC62778.1"
                     /translation="MRHLEVVPDVAEAAARLIAGRRPRTLVLTGGSTPLPLYRRLATM
                     PLPWHEMELFFGDERCVSPDHPDSNYGRAAETLLDHIAARAVYRMRGEDCDAEGYERD
                     LRARFGPGIPAFDFTLLGLGEDGHVASLFPAAPALEERDRLVLRVPHEDHPRLTLTLP
                     VLSASRMVLFLVAGERKRAALARLMAGADIPAARVAAAEVVV"
     gene            complement(613..2031)
                     /gene="zwf"
                     /locus_tag="PLX85_06100"
     CDS             complement(613..2031)
                     /gene="zwf"
                     /locus_tag="PLX85_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRC62779.1"
                     /translation="MALTPPLPQDLLLVGASGDLAQRKLIPGLYNLELDGLLPPEGSI
                     IGYARHGLTDDEFRNFAADAVKRHSRRPFEETAWSSFARRLTFISEEDGGLGAVAERC
                     RQPRRLAYLAIPPSAFMTYIRLLADHGLVDGTRIVIEKPFGNDLATANQLDSELHQYF
                     HEDQIFRIDHYLGKETVQNILVFRFGNSLFERVWNRDAVDHVQITVAESIGVEGRGQF
                     YEEVGALRDIMQNHVFQILSLLTMEPPSSFRAEAIRDEKAKLLESIPPVDPRAVVRGQ
                     YTTGALGDQIARGYRDEPDVSHASNTETFVAMRLNIQNWRWEGVPFYLRTGKRMPETV
                     TEVTVVFREAPIRFFSELPEVSRLRPNQLSIRIQPEESITFSFLAKEPGPEVRVQPVR
                     MHFSYLEAFGAPPPEAYERLLHDAMEGDQTLFLRADGVEEAWRIIEPVLSQMPPLRFY
                     AAGSWGPPQIDDLIEPRRWRLQ"
     gene            2160..2510
                     /locus_tag="PLX85_06105"
     CDS             2160..2510
                     /locus_tag="PLX85_06105"
                     /inference="COORDINATES: protein motif:HMM:NF022114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HRC62780.1"
                     /translation="MGWLRAWLCRLLGCPEERIIITTPAAGVTISSPVAVSGVGQATQ
                     HNQLAVEVRDASNTVIGMGTASVSAPLGQRGPFSASVTFTPTTPGSPGFVQVFDTSPA
                     TGSVTHLASVLIRF"
BASE COUNT          430 a          777 c         1034 g          406 t
ORIGIN      
        1 ggacgaccac ctcggcggcg gcgacgcggg cggccgggat gtcggcgccc gccatgagcc
       61 gggcgagggc ggcacgcttg cgttcgcccg cgacgaggaa gaggaccatg cgcgaggcgg
      121 agagcacggg cagcgtgagg gtcaggcgcg ggtgatcctc gtggggtacg cgcagtacca
      181 ggcggtcgcg ctcctcgagg gcgggagcgg cggggaagag cgaggcgacg tggccatctt
      241 caccgaggcc gaggagagtg aagtcgaagg cggggatacc ggggccgaag cgagcgcgga
      301 ggtcacgttc gtagccctcg gcgtcgcagt cctcgccgcg catgcggtag accgcgcgcg
      361 ccgcgatgtg gtcgaggagc gtctccgccg cgcggccgta gttggagtcg gggtggtccg
      421 gcgaaacgca acgttcgtcg ccgaagaaga gttccatctc gtgccaggga agcggcatcg
      481 tcgccaggcg gcgatagagg ggcagcggcg tgctgccgcc ggtcaggacg agcgtgcgag
      541 ggcggcgtcc cgcaatcaac cgcgcagctg cctcggcgac gtcggggacg acttcgaggt
      601 gtcgcatagt ggctactgga ggcgccagcg gcgcggctcg ataaggtcgt cgatctgcgg
      661 cggcccccag ctgcccgcgg cgtagaagcg cagcggcggc atctggctga gcacgggctc
      721 gatgatgcgc caggcctcct cgaccccgtc ggcgcggagg aagagggtct ggtcgccctc
      781 catggcatcg tgcagcagcc gctcatacgc ctcaggggga ggcgcgccga aggcctcgag
      841 gtaggagaag tgcatgcgga ccggctgcac ccgcacctcg gggcccggct ccttcgcgag
      901 gaaggagaag gtgatggatt cctcgggctg gatgcggatg ctcagttgat tggggcggag
      961 ccggctcacc tcgggcagct cgctgaagaa gcggatcggc gcctcgcgga agacgacggt
     1021 gacctcggtg acggtctcgg gcatgcgctt gcccgtgcgg aggtagaacg gcacgccctc
     1081 ccagcgccag ttctggatgt tgaggcgcat agcgacgaag gtctcggtgt tcgaggcgtg
     1141 gctcacgtcg ggctcgtcgc ggtagccgcg cgcgatctgg tcgcccagcg cgccggtcgt
     1201 gtactggcct cggacaacgg cccgcgggtc gacgggtggg atcgactcga gcagcttggc
     1261 cttctcgtcg cggatcgcct cggcgcggaa ggaggaggga ggctccatcg tgagcagcga
     1321 gaggatctgg aagacgtggt tctgcatgat gtcgcgcagg gcaccgactt cctcgtagaa
     1381 ctggccgcgg ccctcgacgc cgatggactc ggcgacggtg atctgcacgt ggtcgacagc
     1441 gtcgcggttc cagacacgct cgaagagcga gttgccaaag cggaacacga ggatgttctg
     1501 gaccgtctct ttgcccaggt agtggtcgat acggaagatc tggtcctcgt ggaagtactg
     1561 gtgcagctcg ctgtcgagct ggttcgccgt ggcgaggtcg ttgccaaagg gcttctcgat
     1621 gacgatgcgc gtgccatcga ccaggccgtg atcggcgagc agccggatgt aggtcatgaa
     1681 cgcggacggg gggatagcga ggtacgccag ccggcgcggc tggcggcagc gttcggcgac
     1741 ggcgccaagg ccgccgtcct cctcggagat gaaggtcagg cggcgggcga acgaggacca
     1801 cgccgtctcc tcgaaggggc ggcgcgagtg ccgttttacc gcgtcggcgg cgaagttgcg
     1861 gaactcatcg tcggtgaggc cgtgacgggc gtagccgatg atggagccct cgggaggcag
     1921 gaggccgtcg agctcgaggt tgtagagacc ggggatgagc ttgcgctgcg cgaggtcgcc
     1981 tgacgcgccg acgagcagca gatcctgtgg cagtgggggc gtgagcgcca tcggcggtcc
     2041 ttcgcgcgta tttctcgtac atccagcgta tccaagacgg tcgagaaggt ggctacgagg
     2101 cagtaacatc ggcgacgaac gcgcaacgac gttcgtgatt gcctcgaagg aggacgacga
     2161 tgggatggct acgcgcctgg ctgtgccgac tgttgggttg cccggaggag cgcatcatca
     2221 ttaccacgcc cgccgcggga gtgacgatct cgagtccagt ggcagtgtcc ggagtcgggc
     2281 aggccacgca gcacaaccag ctggcggtgg aggtacggga cgcctcgaac acggtgatcg
     2341 ggatggggac ggcgtcggtg agcgcgccgc tgggtcagcg ggggccgttc tcggcctcgg
     2401 tgacgttcac gccgacgacg ccgggatcgc cgggcttcgt gcaggtgttt gacacgagcc
     2461 cggcgacggg gtcggtgacg cacctcgcct cggtcctgat tcggttctag ggacgagtgt
     2521 gggcaaacag atattgatcg cccgggggct cccaccagac ctgcggacgg tagcttcccg
     2581 gcatgccgat ctggcgccag acgcgggtga cccgtgggag ggggacgtcc atcttcatga
     2641 acgcggg
//
LOCUS       DAORPG010000243         2640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4230_length_2640_cov_1.651838, whole genome shotgun sequence.
ACCESSION   DAORPG010000243 DAORPG010000000
VERSION     DAORPG010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4230_length_2640_cov_1.651838"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..720)
                     /locus_tag="PLX85_06110"
     CDS             complement(76..720)
                     /locus_tag="PLX85_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62781.1"
                     /translation="MAEAERTALEFQATLKDVKVDIPKEVSGGFGQKPNGGLLLTLRV
                     DRPKPPAEPRVPYELREPYGPLSLKAIGPEPVRKKKGETDEEFAGRAAAWEREMARRG
                     EALVRHEAATDRWRHQMESWGDVLRAYASITGLAAAFGDSPIQVSLTPLQTDFFAGMG
                     VLSLPAPGDIDAAYEAGVDYDDDEEESFYSDEAEDDSADADEEPAAEEASDPGE"
     gene            complement(720..1001)
                     /locus_tag="PLX85_06115"
     CDS             complement(720..1001)
                     /locus_tag="PLX85_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62782.1"
                     /translation="MRPETVFSRRGFRPVGKCDWCGEDDRVLVAIFLPENAWVCLRSC
                     HVRLERGELGRRAIGGAPFTGERPRRRELRSSELVRVAAEMNGLNEEDF"
     gene            complement(998..1843)
                     /locus_tag="PLX85_06120"
     CDS             complement(998..1843)
                     /locus_tag="PLX85_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62783.1"
                     /translation="MSGRRRPIARPDEPGKALCGIGVPGPSKKRLCGICARLEAGPKV
                     LALDDVTDASQRFGRGDVGFGYGVIWTPERIEAALRAYVRRSSGMLPSGSDEYNALKK
                     GDPSLPVAVTVVKRYGGISRAWLAVGAPKARVSFLGDAWTDEEVEYLLEHAGSQTLRQ
                     IEKRLHRSWSACKRKLYDLGIRARDAQGYMSASQVAAEYSCPQGRVIRLIERGVLKAH
                     RPAGKTNLWAIDPADAKAIEAQLRAPKRTHRPENGYRTDIGDYRAHYGIRRTVLVPLA
                     ERRAS"
     gene            complement(1840..2058)
                     /locus_tag="PLX85_06125"
     CDS             complement(1840..2058)
                     /locus_tag="PLX85_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62784.1"
                     /translation="MSDRYEGTRTLTDWGTFLNHVRDYGKQQIRAIPDDVELEDCLEA
                     ARQNVANWQFDPSHRRHNPVEMPTEDPS"
     gene            complement(2055..2366)
                     /locus_tag="PLX85_06130"
     CDS             complement(2055..2366)
                     /locus_tag="PLX85_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62785.1"
                     /translation="MAITAQAPSRPPAIETTSDLVAALEQTRAKIERLHVITLRVHAE
                     NADLRAEVRGLRMEKERLQGALRRAADDIETCAEEHAADLRVRAAALRREAERGSAAL
                     L"
     gene            complement(2366..>2640)
                     /locus_tag="PLX85_06135"
     CDS             complement(2366..>2640)
                     /locus_tag="PLX85_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62786.1"
                     /translation="GGLVMASKRAQRQHECTRKKPYATHAEAWGVLRAAERAHRVAAG
                     DLCVYRCRNGGEHFHIGHRPAGAYEPTSNQYKALLRNRTRLRGVAA"
BASE COUNT          333 a          997 c          868 g          442 t
ORIGIN      
        1 gcccgcagcc gtcagcagcc cccgccgcct atccgcccag gctggccacc tcctttccgg
       61 tccctccgcg cccggctatt cgccggggtc ggaggcctcc tcggcggcgg gctcttcgtc
      121 cgcatccgcg ctgtcgtcct ccgcctcgtc gctgtagaag ctctcctcct cgtcgtcgtc
      181 gtaatccacg cccgcctcgt aggcagcgtc gatatcaccg ggcgcgggca gcgagagcac
      241 gcccatgccc gcgaagaaat ccgtctgcag cggcgtcagg gatacctgga tcggcgaatc
      301 accaaaggcg gcagccagcc cggtgatgga ggcatatgcg cgcagcacgt cgccccacga
      361 ctccatctga tgccgccagc ggtccgttgc cgcttcgtgg cgtacgagcg cttcacctcg
      421 gcgcgccatc tcccgctccc acgcggcggc gcggccggcg aactcctcgt ccgtctcgcc
      481 cttcttcttg cgtaccggct cggggccgat tgctttcagc gagagcggtc catacggctc
      541 ccggagctcg tacggcaccc gcggctctgc cggcggcttt gggcggtcga cgcgcagcgt
      601 tagcaacaac ccgccgttcg gcttctggcc aaacccgccc gagacctctt tcgggatgtc
      661 gaccttgacg tccttcaacg tcgcctggaa ctcgagcgct gtgcgctccg cctccgccat
      721 cagaagtcct cctcgttcaa cccgttcatc tccgcagcga cgcgcaccag ctcggacgac
      781 ctcagctctc ggcggcgcgg ccgctcaccc gtgaacggcg cgccgccgat ggcccggcgt
      841 ccgagctctc cgcgctccag ccgcacatgg cagctgcgca ggcacaccca cgcgttctcg
      901 ggcaggaaga tcgccaccag gacgcgatcg tcctcgccgc accagtcgca cttcccgacc
      961 gggcggaacc cgcggcgcga gaacaccgtc tccggcctca cgacgccctc cgctcagcga
     1021 gcgggacgag gaccgtgcgg cggatgccgt agtgcgcccg gtagtcaccg atgtcggtgc
     1081 ggtagccgtt ctcgggtcgg tgggtgcgct tcggggcgcg cagctgcgcc tcgatggcct
     1141 tcgcgtccgc cgggtcgatc gcccagaggt tggtcttgcc ggccggccgg tgcgccttca
     1201 acacaccgcg ctcgatcagc cggatgacgc gcccctgcgg gcaggagtac tcggccgcga
     1261 cctgcgacgc cgacatgtag ccctgggcgt ctcgcgcgcg gatcccgagg tcgtagagct
     1321 tccgcttgca ggcgctccat gaccggtgca gccgcttctc gatctgccgg agggtctggc
     1381 tgcccgcgtg ctcgaggagg tactcgacct cttcgtccgt ccaggcgtcg ccgaggaagc
     1441 tgacccgcgc cttcggcgct cctaccgcca gccaggcgcg ggagatgccg ccgtaccgtt
     1501 tgacgacggt caccgcgacg ggcagcgagg ggtcaccctt cttgagcgcg ttgtactcgt
     1561 ccgatccgct cgggagcatg cccgaggagc ggcggacgta ggcgcgcagc gcggcctcga
     1621 tccgctcggg cgtccagatg acgccgtacc cgaagccgac gtcgcctcgc ccgaagcgct
     1681 ggctggcgtc cgtgacgtcg tcgagcgcga gcaccttcgg ccccgcttcg aggcgcgcgc
     1741 agatgccgca cagccgcttc ttcgacggcc cgggcacccc gatcccgcag agcgccttcc
     1801 ccggctcgtc cggccgcgcg atggggcgcc gacgtccgct cacgaaggat cctccgtggg
     1861 catctcgacg gggttatggc gccggtgcga ggggtcgaac tgccagttcg cgacgttttg
     1921 ccgcgcggct tccaggcagt cttcaagctc cacgtcgtcg gggatggcgc ggatctgctg
     1981 tttgccgtag tcgcggacgt ggttgaggaa cgtgccccag tccgtcagcg tccgagtacc
     2041 ttcgtagcgg tcgctcacag caacgccgcc gatccccgct ctgcctcccg gcggagggca
     2101 gccgcgcgca cgcgcaagtc cgcagcgtgc tcctcggcac aggtctcgat gtcatccgcg
     2161 gcccggcgca acgcgccctg cagccgctcc ttctccatgc gcagcccgcg cacctcggcg
     2221 cgcaggtcgg cgttctcggc gtgcacccgc agcgtgatga cgtgcaggcg ctcgatcttc
     2281 gcgcgcgtct gttcgagggc agcgaccagg tcggacgtcg tctcgatcgc cggtgggcgc
     2341 gacggtgctt gcgctgtgat cgccattagg ccgccacccc tcgcagccgc gtccggtttc
     2401 ggagcagcgc cttgtactgg ttcgacgtcg gctcgtacgc gcccgcaggg cggtggccga
     2461 tgtggaagtg ctcgcctcca ttgcggcagc ggtagacgca gaggtctccg gcggcgaccc
     2521 gatgcgcccg ctcggcggcc cgcaacaccc cccaggcctc ggcgtgggtg gcgtagggct
     2581 tcttcctcgt gcactcgtgc tggcgttgcg ctcgcttgct ggccatcacg aggcctcctc
//
LOCUS       DAORPG010000244         2638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4238_length_2638_cov_2.730933, whole genome shotgun sequence.
ACCESSION   DAORPG010000244 DAORPG010000000
VERSION     DAORPG010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4238_length_2638_cov_2.730933"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PLX85_06140"
     CDS             <1..314
                     /locus_tag="PLX85_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRC62787.1"
                     /translation="LERRGHRVIARNVRRREGEIDLVTVDGDTLVFVEVKLRRPRDFG
                     RAVEALTAAKRRRLQTLAEAYTAEHPELPRTQRIDLVAIELAADGSVGDVRHIESAVE
                     G"
     gene            354..797
                     /locus_tag="PLX85_06145"
     CDS             354..797
                     /locus_tag="PLX85_06145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRC62788.1"
                     /translation="MPLYQFDCSGCEKRVEILFRSVTTTKKPVCPECGSKKLKKVMSR
                     VARIKSTRERFEDIDHVREAAALGGSSDPAVFARWARRVGAEYDEELGTNYRELAERA
                     EAGETVHERVDADHTFRHQVNQKLASMDMPADVPPVDSGGHDHVH"
     gene            830..1159
                     /locus_tag="PLX85_06150"
     CDS             830..1159
                     /locus_tag="PLX85_06150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRC62789.1"
                     /translation="MPIYEYKCAKCEHVTSVFVRSATATVSPKCEACGAKKLTRQMSR
                     VARLRTARDVQEEYSRPEDANRDPRQIGQWVEERFQKYGMNLPKDAREAIEKAREGQM
                     PGPVDDL"
     gene            1268..1687
                     /locus_tag="PLX85_06155"
     CDS             1268..1687
                     /locus_tag="PLX85_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62790.1"
                     /translation="MTQATFSGSGTAADPWVLKTPPGSSEFTMHREPEADPPALVCQV
                     GKTQLRYHLRCIEDLHSWLQAQGDWVALGGTDEQKEAPAGSVEAWGRDAANPVGGWYG
                     LKKGLRGRFGVYVPPVLEALGLAEVEHNARNNRMRAL"
     gene            complement(1710..1880)
                     /locus_tag="PLX85_06160"
     CDS             complement(1710..1880)
                     /locus_tag="PLX85_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62791.1"
                     /translation="MRKLLLIAAGLAIGAAVAVVLSAREQLDSPSTPASDAHQRWPEV
                     PAPPLSETPASG"
     gene            2015..>2638
                     /locus_tag="PLX85_06165"
     CDS             2015..>2638
                     /locus_tag="PLX85_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRC62792.1"
                     /translation="MTESPTPTPPSADLETITSLAKRRGFAFPSAEVYGGLSSTYDYG
                     PLGVEMKRAIRELWWRRMVQERDDVVGIEAAIITNPAVWQASGHVAGFTDPLVDCLEC
                     HSRVRADHIEHNTCPVCGTVGRFTEERQFNLLFRTFIGPVQDEAATAYLRPETAQGMF
                     VNFENVQTTTRKRLPFGIAQIGKSFRNEITPGQFIFRTREFEQMEMEY"
BASE COUNT          452 a          810 c          990 g          386 t
ORIGIN      
        1 acctcgagcg gcgcgggcat cgggtgatcg cgcgcaatgt gcgacgccgc gagggagaga
       61 tcgacctcgt cacggtcgac ggcgacacgc tcgtgttcgt cgaggtgaag ctgcggcggc
      121 cgcgcgactt tgggcgggcg gtcgaggcgc tgacagcggc gaagcggcgg cgactgcaga
      181 cgctcgccga ggcgtacaca gccgagcatc cggagctgcc tcgcacgcag cggatcgacc
      241 tcgtggcgat cgagctggcg gccgacggtt cggtagggga cgtgcggcac atcgaaagcg
      301 ccgtggaggg gtagcgtctg gcgagcgccg cgcggccgtc ctacactcgg gccatgccgc
      361 tgtatcagtt cgactgttcc gggtgcgaaa agcgggtcga gatcctgttc cgctcggtga
      421 cgacgacaaa gaagccggtc tgtcctgagt gcgggagcaa gaagctcaag aaagtgatgt
      481 cgcgcgtcgc gcgcatcaag tcgacacgcg aacgctttga ggacatcgac cacgtgcgcg
      541 aggccgcggc gctgggtggc agcagcgatc cggccgtctt cgcgcgctgg gcgcgtcgtg
      601 tgggggccga gtacgacgag gaactcggca ccaactaccg cgagctggcg gagcgggccg
      661 aggccgggga gaccgtccac gagcgcgtcg acgcggacca cacgttccgc caccaggtga
      721 accagaagct tgcctcgatg gacatgccgg cggatgtgcc gcccgttgac tcgggcgggc
      781 acgaccacgt ccattagcgc gcggagcgcg tacccgggag gaggtgcgca tgccgatcta
      841 tgagtacaag tgcgcgaagt gcgagcacgt cacctcggtg ttcgtgcggt cggcgactgc
      901 cactgtgtcg ccgaagtgcg aggcatgcgg ggcgaagaag ctgacgcggc agatgtcgcg
      961 ggtcgcccgg ctgcggaccg cgagggacgt gcaggaagag tacagccggc ccgaggacgc
     1021 caaccgcgac ccccggcaga tcgggcagtg ggtcgaggag cgcttccaga agtacggcat
     1081 gaacctgcca aaggacgcgc gggaagccat cgagaaggcc cgcgaggggc agatgcctgg
     1141 ccccgtcgac gacctgtagc ggctccgagc gctctcggct cgattccgcg acccctgcgc
     1201 cgttgtgtcc ttcgtttggc gtctgttacc gtaactactg cgttacgcat tgacgaggga
     1261 ggtggcgatg acgcaggcga cgttttccgg ctcgggcacg gcggcggacc cctgggtgct
     1321 gaagacgccg ccgggttcct cggaattcac gatgcaccgc gagcccgagg ccgatccgcc
     1381 tgccctcgtg tgccaggtgg ggaagacgca actgcgctac cacctgcggt gcatcgagga
     1441 tctgcactcg tggctccagg ctcagggcga ctgggtggcc ctcggcggga cggacgagca
     1501 gaaggaggcg ccggcgggca gcgtcgaggc ctggggacgc gacgccgcga acccggtcgg
     1561 cgggtggtat ggactgaaga aggggctacg ggggcggttc ggcgtgtacg tgccgccggt
     1621 gctcgaggcg ctgggattgg cggaggtcga gcacaacgcg cggaacaacc ggatgcgcgc
     1681 actgtaggct ggcgactcgc cgacgcctat tagcctgaag cgggggtctc cgacaacgga
     1741 ggggctggaa cctcgggcca ccgctggtga gcgtcggatg caggcgttga gggcgagtcg
     1801 agctgctcgc gcgccgagag cacgacggcg acggcggcgc cgatggcgag accggcggcg
     1861 atcaagagca gcttgcgcat cggggactcc gttcgggcgc gatcgtacca cgcgctgctc
     1921 gacccgctcg aagcgagctc ggacggtcat ttcttgaggg cagagggggc gaatgctacg
     1981 ctcgcggccc gaccgtccgt agcctggagt gagtgtgacc gagagcccga cgcccacgcc
     2041 cccgagcgct gacctcgaga cgattacatc gctggcgaag cgccgcgggt tcgcgtttcc
     2101 ctccgccgag gtgtacgggg gcctctcctc gacgtacgac tacggaccgc tgggcgtgga
     2161 gatgaagcgc gcgattcgcg agctgtggtg gcggcgcatg gtgcaggaac gcgacgatgt
     2221 cgtcggtatc gaggccgcca tcatcacgaa ccccgctgtg tggcaggcct cggggcacgt
     2281 cgcgggcttc acggacccac tggtcgactg cctcgaatgc cactcacgcg tgcgcgcgga
     2341 ccacatcgaa cacaacacgt gtccggtctg cgggaccgtg gggcgcttca ccgaggagcg
     2401 gcaattcaac ctcctcttcc ggacgttcat cgggccggtg caggacgagg cggcgacggc
     2461 atatctgcgg ccggagacgg cgcagggcat gttcgtgaac ttcgagaacg tgcagaccac
     2521 gactcggaag cggctcccgt tcgggatcgc gcagatcggg aagtcgtttc gcaacgagat
     2581 cacgccgggg cagttcatct tccggacgcg cgagttcgag cagatggaga tggagtac
//
LOCUS       DAORPG010000245         2636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4245_length_2636_cov_3.484308, whole genome shotgun sequence.
ACCESSION   DAORPG010000245 DAORPG010000000
VERSION     DAORPG010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4245_length_2636_cov_3.484308"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLX85_06170"
     CDS             complement(<1..320)
                     /locus_tag="PLX85_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing (seleno)protein"
                     /protein_id="HRC62793.1"
                     /translation="MTATIIQGETTFESAQATFRDGDLWAPLAEVTAALGWERKPEGI
                     CREDICIPVPEGREAALLGADGREFNLSEFARLIEQPVAASEEFGAAYFGAPSWQWQH
                     PLAGG"
     gene            424..1755
                     /locus_tag="PLX85_06175"
     CDS             424..1755
                     /locus_tag="PLX85_06175"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC62794.1"
                     /translation="MAQLIRTADAPAEPSVIEPFEAAAEAEDDSDRSAAVAEPRTGRF
                     AALAVLSQRDFRFLFLSSIATGYAQWGQMIGMSWLVYELTGRSAVHLAAVNFVGGFGR
                     LLIGPFVGVALDRWHRRSILMTSTMLSAVQALLLAVLVLTDLVAVWQVFIFAALDALL
                     STTNQNARQAFVYDVTGPEALPDAIAVNSMMQNITRITGPTLAGALIGAWGTAAPFLM
                     IATTMTLGVLTTLPISRQTRQGARATGHPLANLRDGFVYVARDRRLLGLTVVMSFTAL
                     LVYPYLTFLPIFAAEVLHAGSGGYGTLAAAAGIGSLLGLFGLVVMRRVEHRAVVLMFG
                     LLFYFVCLIAFTRATNLWIAAALLAVGGMPHAIAQTMLQMLQQLLPVNEMRGRVTGVV
                     QTAFALYPLGALPMGFAISRWGAPDTCLVSFSAATVLLLCAITGWKSLRTV"
     gene            1775..>2636
                     /locus_tag="PLX85_06180"
     CDS             1775..>2636
                     /locus_tag="PLX85_06180"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRC62795.1"
                     /translation="MSLADRTPPTAEEYASYRERFSNWGRWGADDRLGTLNHITPEVR
                     TRAAGLVRDGRTVSLALKLNGPTRAALPSGFRQTMRVGEASSGDQLDINFHGWSITHL
                     DALCHIFTGPGGALYNGRPASDVTEGGAKSSDVSAYAGGLVTRGVLYDVPRFRGTPHV
                     TLDTPVHGWELVDVAASQGVRPASGDAVVIRSGANAFYAATPDFGMGDIERMPGGHAS
                     VLEFLHETDASVLVWDLLDHGGQPYPGGLPMGGRDVAIPIHEIAIPYMGMPLLDNADL
                     DALAATCEELG"
BASE COUNT          370 a         1021 c          799 g          446 t
ORIGIN      
        1 ccgccggcca gcggatgttg ccactgccac gagggagccc cgaagtaggc cgccccgaac
       61 tcctcgctcg ccgcgacggg ttgctcgatc agccgcgcga actcgctgag gttgaactcg
      121 cgcccgtctg cccccagaag cgctgcttcc cgcccttccg ggaccgggat gcagatgtcc
      181 tctcggcaga tgccttccgg cttgcgttcc cagccgagcg ccgccgtaac ctcagcaagc
      241 ggcgcccaca ggtcgccgtc ccgaaacgtc gcctgggccg actcgaacgt cgtctccccc
      301 tggatgatgg tcgcagtcat cgcctccccc tctggcaaat cacgaggagc gtagcatccg
      361 gccaccgccc tcgtgcagca cgtcgaggag caggcgaacg aggtgagtgc tataacacct
      421 cgaatggctc aactcattcg caccgccgac gcaccggccg aaccctcggt catcgaaccg
      481 ttcgaggctg cggccgaagc ggaggacgac tctgaccgca gcgcggcggt cgccgagccg
      541 cggactggtc gcttcgccgc gcttgccgtc ctcagccagc gcgacttccg cttcctgttc
      601 ctctcctcga tcgccaccgg ctacgcgcag tgggggcaga tgatcgggat gagctggctc
      661 gtctacgagc taaccggccg ttccgccgtg cacctcgccg cggtgaactt cgtcggcggc
      721 ttcggccgcc tcctgatcgg gccgttcgtc ggcgtcgcgc tcgaccgctg gcaccgccgg
      781 tcgatcttga tgacgtcgac gatgctcagt gcagtccagg cgctgctgct ggccgtcctc
      841 gtcctgaccg acctcgtcgc cgtctggcag gtcttcatct tcgctgccct cgacgccctc
      901 ctgagcacga ccaaccagaa cgcccgccag gccttcgtct acgacgtcac cggccccgag
      961 gcgctccccg acgcaatcgc ggtcaactcg atgatgcaga acatcacgag gatcaccggc
     1021 ccgacgctcg cgggcgccct catcggcgcc tggggcacgg cggcgccgtt cctcatgatc
     1081 gccaccacca tgacgctcgg cgtcctcacc acgctgccga tcagccgcca gacgaggcag
     1141 ggcgcgcgag cgaccggcca tccactcgcc aacctccgcg acggcttcgt ctacgtcgcg
     1201 cgcgaccgcc gcctcctcgg cctcacggtc gtcatgagct tcacggcgct cctggtctac
     1261 ccatacctga cgttccttcc gatcttcgcg gccgaggtgc tccacgccgg ttccggtggc
     1321 tacggcacgc tcgcggcggc cgccggaatt gggtccctcc tggggctatt cgggctcgtg
     1381 gtgatgcgcc gcgtcgaaca ccgagcggtc gtgctcatgt tcgggctgct gttctacttc
     1441 gtctgcctga tcgccttcac ccgcgcgacg aacctctgga tcgccgcggc gttgctcgcc
     1501 gtcggcggca tgccgcacgc catcgcccag acgatgctcc agatgctcca gcagctcctc
     1561 cccgtcaacg agatgcgcgg ccgtgtgacc ggcgtcgtgc agaccgcgtt cgcgctctac
     1621 ccgctcggcg cgctccccat gggcttcgcc atctcgaggt ggggcgctcc cgatacctgt
     1681 ctcgtttcct tctccgccgc caccgtcctg ctgctctgcg ccatcaccgg ctggaagtcg
     1741 ctccgcaccg tctgaagtac ctggaggtcc gacaatgagc ctcgccgacc gcaccccgcc
     1801 caccgccgag gagtacgcct cctaccgcga gcgcttcagc aactggggcc gctggggcgc
     1861 cgacgaccgc ctcgggaccc tcaaccacat cacccccgag gtgcggactc gggctgcggg
     1921 gctcgtgcgc gatggccgca cggtctcgct cgcactcaag ttgaacggcc cgacccgcgc
     1981 ggcgctgccg tcgggattca ggcagacgat gcgggtcggc gaggccagct ctggcgacca
     2041 gctcgatatc aacttccacg gctggtcgat cacccacctc gatgccctct gccacatctt
     2101 cacgggcccg ggcggcgccc tctacaacgg gcggcccgcc tccgacgtca ccgagggcgg
     2161 cgcgaaaagc agcgatgtct cggcctacgc cggcggcctc gtcacgcgcg gcgtcctcta
     2221 tgacgtcccc cgctttcgag gcacgccgca cgtcacgctc gacacgccgg tgcacggctg
     2281 ggagctcgtc gacgtcgccg cctcccaggg cgtccggccc gcgtctggag acgcggtggt
     2341 cattcgctcc ggtgccaacg ccttctacgc cgcgacgccc gacttcggca tgggcgacat
     2401 cgagcggatg ccgggcggtc acgcctctgt gcttgagttc ctccatgaga cggatgcctc
     2461 cgtcctcgta tgggacctcc tcgatcacgg tggccagccc taccccggcg gactgccgat
     2521 gggcggccgc gacgtcgcca ttccgatcca tgagatcgcc atcccgtaca tgggtatgcc
     2581 actgctcgac aatgccgacc tcgacgctct tgccgccacg tgcgaagagc tcggcc
//
LOCUS       DAORPG010000246         2629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4271_length_2629_cov_3.261849, whole genome shotgun sequence.
ACCESSION   DAORPG010000246 DAORPG010000000
VERSION     DAORPG010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4271_length_2629_cov_3.261849"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PLX85_06185"
     CDS             complement(<1..745)
                     /locus_tag="PLX85_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC62796.1"
                     /translation="MGFAQLVLRNLLRARVRSGLTLVGISLGITTIVALGVITAGFKS
                     TAGAFVQSGGADFMVAQEGAADMSFSTIPETVVPQIAAVSGVAAARGALFHFTRAGSN
                     PLFLLLGVTPGDLAADGPPLVEGRYLADGATDEIVLGAQGATDLNVRVGDALTIDDRV
                     FHVVGVVQSDVLWEQSGALAPLVTVQEIANRREAVSVVHITLASGTKRDEVARRIEDE
                     VPGVVSIVTADDIGKIDSGFQLLDAANVAI"
     gene            complement(797..>2629)
                     /locus_tag="PLX85_06190"
     CDS             complement(797..>2629)
                     /locus_tag="PLX85_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRC62797.1"
                     /translation="FLVLTIALTVLGVAQGRDLRLTIEMAVALAIGAVPEGLPAVATT
                     TLAVAVRRLATRRVLVRRLNAVETLGSTTVIVSDKTGTLTENRLVVRSVLLPSGERWE
                     FPRDLHPEHDEQGRPRVPSEAADEAARVLLIAALCGEAIVEYDDEDGWHAHGDPLEAA
                     ISYAAAALGWHTDELHERFPRRQTTPFTPERRYMQTEHATEDGPLLAVKGAPAQVFAL
                     ATGDPRLLRATNELGEEGYRVLAVAAGPIEADLEVIGAVVLEDPLRADAAEAVAASRA
                     AGLRLMLATGDQLTTATTIARESGLLRAGETAVEAASLDGADPASVAVVARATHQQKA
                     ALVELLQRSGEVVAMLGDGVNDAPALRAADVGVAVGPGASDVAVEASEVILADGRLAS
                     LIEGIREGRTTALNLRRAIVYLLTASFATIGAVGLGMLLDDQLPMGAIQILWLNLVVH
                     VFPAIALATGRGTGNLGAPTRTLLAQATWVEIGARAAVVAVLAGAVFEWSVRQGGAAQ
                     HQQTLVFLIIALSLLGQSFLIGVRSPRELPRALRRVELWYALAMSTAFLLFAFYVPFM
                     RDALEVEIPSLRDWASVLALAFAAGNATQIATWLLARTEPESRA"
BASE COUNT          421 a          875 c          973 g          360 t
ORIGIN      
        1 agatcgcgac gttcgcggca tcgaggagct ggaagccgct gtcgatcttg ccgatgtcat
       61 cggcggtcac gatggagacg acgccgggga cttcgtcttc gatgcgtcgg gccacctcgt
      121 cgcgcttggt gccggaggcg agcgtgatgt ggaccacgct gaccgcctcg cggcggttgg
      181 cgatctcctg caccgtcacg agcggggcaa gagcgcccga ctgctcccat aacacgtccg
      241 actgcacgac gcccacgacg tggaagacgc ggtcatcgat cgtgagggcg tcgcccacac
      301 ggacgttcag atcggtcgcg ccctgggcgc ccagcacgat ttcgtcggtc gcgccgtccg
      361 cgagatagcg gccttccacc agcggcggac cgtccgcggc cagatccccg ggcgtcacgc
      421 cgaggagcag gaacagcgga tttgagccgg cgcgcgtgaa gtggaatagc gcaccgcggg
      481 cggcggcgac cccggagacg gccgcgattt gcgggacgac cgtctcgggg atcgtgctga
      541 aactcatgtc ggcggcaccc tcctgggcaa ccatgaagtc ggcaccgccc gactggacga
      601 acgcaccggc ggtcgacttg aagccggccg tgatgacgcc aagcgcgacg atggtggtga
      661 tgccgaggct gatgcctacg agtgtgagcc ccgaacgtac tctcgcccgg aggagatttc
      721 gcagcaccag ttgagcgaag cccatgggcc gaatcgtagc gccgtcaacg cgggacgggc
      781 ggtgccaaac ggtaggtcat gctcgggact ccggctcggt gcgggcgagc agccaggtcg
      841 cgatctgagt ggcgtttccg gcagcgaacg cgagcgccag aacggaagcc cagtcacgca
      901 ggctcgggat ttcgacctcg agcgcgtcgc gcatgaacgg gacgtagaac gcgaagagca
      961 ggaacgccgt actcatcgca agggcgtacc acagctcgac gcggcgcagg gcgcgtggca
     1021 gctcgcgtgg cgagcggaca ccgatcagga acgattggcc gaggagtgac agcgcgatga
     1081 tgaggaagac cagcgtctgc tggtgctgcg ccgccccgcc ctggcgcaca ctccactcga
     1141 acaccgcgcc cgcgaggacc gcgacgaccg cggcacgcgc gccaatctcg acccaggtgg
     1201 cctgggcgag cagggtgcgg gtgggggcgc cgaggttgcc cgtgcctcgc ccggtcgcga
     1261 gtgcgatcgc cggaaagacg tggacgacga ggttgagcca caggatctgg atcgcgccca
     1321 ttggcagctg gtcgtccagc agcattccga ggccaactgc gccgatggtg gcgaagctcg
     1381 cggtcagcag gtagacgatc gctcggcgca ggttgagcgc agtcgtgcgc ccctcgcgaa
     1441 taccctcgat gagggacgcg aggcggccgt cggcgaggat gacctcggac gcctcgaccg
     1501 cgacgtcgct cgcgccgggg ccgacggcga ccccaacgtc cgcggcccga agggccggcg
     1561 cgtcattgac gccgtcgccg agcatggcga cgacctcgcc actccgttgc agcagctcga
     1621 cgagcgccgc cttctgctga tgggtcgcgc gcgcgacgac cgcgaccgac gcagggtcgg
     1681 cgccgtcgag ggacgccgct tcgaccgcgg tctccccggc gcggaggagt ccggactcgc
     1741 gggcgatcgt cgtggcggtg gtgagctgat cgcccgtcgc gagcatcagg cgcagtccgg
     1801 cggcccttga cgccgctacg gcctcggcgg catcagcgcg caacgggtct tccaggacga
     1861 cggcgccgat cacctcgagg tcggcctcga tcgggccggc cgcgaccgcg aggacgcggt
     1921 acccctcctc gcccagctcg ttggtcgcgc ggaggagccg aggatcgccc gtggcgaggg
     1981 cgaagacctg cgctggcgcg cctttgacgg cgagcaatgg gccgtcctcc gtcgcgtgct
     2041 cggtctgcat gtagcgccgc tcgggggtga agggcgtggt ctggcggcgc gggaagcgct
     2101 cgtgcagctc atcggtgtgc cagccaaggg cagcggccgc gtacgagatc gccgcctcga
     2161 gtgggtcgcc gtgcgcgtgc cagccgtctt cgtcgtcgta ttccacgatg gcctcgccac
     2221 agagcgcggc gatgaggagc acgcgagccg cctcgtccgc cgcctcggaa ggaacgcgcg
     2281 gccgtccctg ctcgtcgtgc tccgggtgca ggtcacgcgg gaactcccag cgctcgcctg
     2341 atggaagcaa caccgaacgg acgacgaggc ggttctcggt gagggtgccc gtcttgtcgc
     2401 tgacgatgac cgtggtggag ccaagcgtct cgaccgcgtt caggcggcgg acgaggactc
     2461 gtcgggtggc caggcggcgc accgcgacgg cgagcgtggt ggtggcaacg gccggcaagc
     2521 cctcggggac ggccccgatg gcgagggcga ccgccatctc aattgtcagc cggaggtcgc
     2581 ggccctgcgc gacgcccagc accgtcagtg cgatggtgag cacgaggaa
//
LOCUS       DAORPG010000247         2626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4279_length_2626_cov_2.328666, whole genome shotgun sequence.
ACCESSION   DAORPG010000247 DAORPG010000000
VERSION     DAORPG010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4279_length_2626_cov_2.328666"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PLX85_06195"
     CDS             complement(<1..649)
                     /locus_tag="PLX85_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62798.1"
                     /translation="MVESSLDEAQFEREETARQNLIRQCMAARGFSYTPVTPQHVDSA
                     STAPAESGVDPNLSWVMSLDAKTRAAHYRALYGTDNPFEPTASATPEGCATQAYARIP
                     GVFALSSTLSAELEDLRRAMKNDPRVVAADARWGDCMAAQGFAVSSREEMLRGVDSSP
                     AGDRSALAADRTHCAAGLDEVRNTVRIAYENAFAAAHAVELRAHQERLAADEALVL"
     gene            complement(808..1503)
                     /locus_tag="PLX85_06200"
     CDS             complement(808..1503)
                     /locus_tag="PLX85_06200"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRC62799.1"
                     /translation="MAAVALGLASALFLDDVPASADTVYEPYITRNAYKIYLSPAPQP
                     GNGACGVDESTQARTAALAATSSAYQDGYYYPSPAMQANLGARGYRVRVGSGDYLANR
                     NSSNAGGAHRHIPVHTNAHVGTIVCSRTDAANSGTQVYYSTGRTTGQNLANKLKDTVG
                     VKSPGTGDKVVTNTSWAELNSVTAPAAYLESEFHTWQRGADWIKTSTVMWGGVWPTPS
                     TFTSAIREVRTRT"
     gene            complement(1711..>2626)
                     /locus_tag="PLX85_06205"
     CDS             complement(1711..>2626)
                     /locus_tag="PLX85_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1727 domain-containing protein"
                     /protein_id="HRC62800.1"
                     /translation="DPDSFAVWGFEQLLHHRIPLYKAIADNFGYTVRMRDVPHVTSDD
                     SFVARIRPSIPAGVQVRWFGIDPTLVDGLPSLYEADVRDDAGVEPEPLASGDAMLRPR
                     LPDAFTVSFGDGVDLGPVTLAQPGLAAMINATAAVAMARVLLGDRFDIRKTEQALREL
                     EPAFGRGEVIDADGAPLELVLVKNPAAFTVALGTYGDRPVATMVAINDNDADGEDVSW
                     LYDVSFASLRAGGVALTSGQRAYDMALRLQYDDVPVAAVEPDLEAGLDRFLEELPGRP
                     RRIFCTYTAMMELRLLLQTRYGLANFGE"
BASE COUNT          429 a          882 c          875 g          440 t
ORIGIN      
        1 ccaacaccag ggcttcgtcc gccgccaagc gctcctgatg ggcgcgcagc tcgaccgcgt
       61 gggcggctgc gaaggcattc tcgtaggcga tccgcactgt gttgcgaact tcgtccagcc
      121 cagcggcgca gtgggtgcga tccgcggcca gcgctgagcg gtctccagcg ggcgaggagt
      181 ccactccacg gagcatctct tcgcgagagg acacggcaaa cccctgggct gccatgcagt
      241 cgccccacct ggcatcggca gcgaccaccc gggggtcgtt cttcatcgcg cggcgcaaat
      301 cctcaagctc ggcgctcagt gtgctcgaga gcgcgaacac cccggggatc cgggcgtaag
      361 cctgagttgc gcacccttcc ggcgtcgcac tcgccgtcgg ttcgaagggg ttgtccgttc
      421 cgtagagggc ccgatagtga gccgcccgcg tcttcgcgtc gagggacatg acccagctga
      481 ggttggggtc gacacccgat tcggctgggg ccgtggacgc gctgtcgacg tgctgcggcg
      541 tcaccggggt gtagctgaac ccccgcgcgg ccatgcactg gcggataagg ttctgtcgag
      601 ccgtctcctc gcgctcgaac tgggcttcgt cgagggagga ctcgaccatc cggaatcccg
      661 ggacggcttc tagtgcagag gtcgccaccc gagcagcact tgctagtgac gccggctgat
      721 ctggcgctct caccgcagct tgtccaccga cgagagcccc cacccccatg gccgacatcg
      781 ctactgcgag aatccatggc ttgcgcttca tgtccgggtc ctaacctctc ggatagccga
      841 ggtgaaggtc gacggcgtag gccacacgcc accccacatc accgtggacg tcttgatcca
      901 gtcggcgcct ctttgccagg tgtggaactc agactccagg taggcagcgg gcgcagtgac
      961 agagttcagc tcagcccatg atgtattggt gactactttg tcacccgttc ccgggctctt
     1021 caccccgact gtgtccttta gcttgttcgc caggttctga ccagtcgtcc ggccggtgga
     1081 gtagtacacc tgcgttccac tattcgccgc gtcggtgcgt gagcagacga tcgtgccgac
     1141 gtgggcgttt gtgtgcacgg gtatgtggcg gtgggcaccc ccagcattgg agctgtttcg
     1201 gttcgcgagg tagtctcccg aacccacccg gacccgatag ccgcgcgcgc ccaggttcgc
     1261 ctgcatggca gggctcgggt agtagtagcc gtcctgatag gcagacgagg tcgcagcgag
     1321 tgcggccgtg cgcgcctggg tgctctcgtc gacaccgcac gccccattgc caggttgcgg
     1381 tgcaggcgac aggtagatct tgtacgcatt ccgggtgata tagggctcgt agactgtgtc
     1441 agccgaagcg ggcacatcat cgaggaacaa ggcggaggca aggccgagtg ccacggctgc
     1501 cacgcaacgc ccacgacgca acctcataac gcacccctgt cgtgtcggct cgttgggtct
     1561 gtcgggtcaa ctcgatgctt ccgacggtag ccccccccaa gaaagccgcc tcggaaatgt
     1621 gtccgccgtc acaaccgatg gaaggggcgt gcgctcagtg cgcgatcaac agctgagtgt
     1681 cgccgcgcac tccagcgccg caggtctgac ctactcgccg aagttggcca ggccatagcg
     1741 ggtctggagc agcaggcgca gctccatcat cgccgtgtag gtgcagaaga tccgtcgcgg
     1801 ccgcccgggg agctcctcga ggaagcggtc cagccccgcc tccaggtcgg gctcgaccgc
     1861 cgcgaccggg acgtcgtcat actgcagccg cagggccatg tcgtacgccc gctgaccgga
     1921 ggtgagcgcc accccacccg cgcgcaggct cgcgaaggag acgtcgtaga gccacgagac
     1981 gtcctcgccg tcggcgtcgt tgtcgttgat cgcgaccatc gtcgccaccg gccggtcgcc
     2041 gtaggtgccc agcgcgacgg tgaaggcggc cgggttcttg accaggacca gctccagcgg
     2101 cgcaccgtcg gcgtcgatca cctcgccgcg cccgaaggcc ggctccagct cgcgaagggc
     2161 ctgctcagtt ttccgtatgt cgaaccggtc gccgagcagc acccgggcca tcgccaccgc
     2221 agcggtcgca ttgatcattg ctgccaggcc gggctgggcc agggtcaccg ggccgaggtc
     2281 gacgccatcg ccgaaggaga cggtgaaggc gtccgggagg cgcggacgca gcatggcatc
     2341 gccgctcgcg agcggctcgg gctcgacacc ggcgtcgtcg cggacgtccg cctcgtacag
     2401 ggacggcagg ccgtcaacca gcgtcgggtc gatcccgaac caccgcacct gcaccccggc
     2461 ggggatgctc ggccggatcc gggcgacgaa ggagtcgtcg ctggtgacat gcggcacgtc
     2521 acgcattcgg accgtatagc cgaagttgtc tgcaatcgcc ttgtagagcg ggatgcggtg
     2581 atgcagaagc tgctcgaagc cccacaccgc gaagctgtcg ggatcg
//
LOCUS       DAORPG010000248         2620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4290_length_2620_cov_3.953606, whole genome shotgun sequence.
ACCESSION   DAORPG010000248 DAORPG010000000
VERSION     DAORPG010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4290_length_2620_cov_3.953606"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..417
                     /locus_tag="PLX85_06210"
     CDS             157..417
                     /locus_tag="PLX85_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HRC62801.1"
                     /translation="MPEVVLYGQPGCHLCEDAEAALEALCRSRPFALRVVDINSDDDL
                     ARRYVFEIPVIEVDGAVVTRAPVDLRAVTAALDAPASTEGRR"
     gene            complement(424..1338)
                     /locus_tag="PLX85_06215"
     CDS             complement(424..1338)
                     /locus_tag="PLX85_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62802.1"
                     /translation="MAIQVGEMFHVIHMYDELGPLDEWYDKIFAVKRSMHGNYSEVEK
                     RDASLAFIADFCMEPMAPAYRVEGAEDMPVGRFHRRFGNRLHSVAWYIDGGFGELFGK
                     LKDGGVRLFTDGGGAATSADNLVAAAFTHPRDTGCQWELMPRREGMSIGTGAIPGYDE
                     DFWPNEHPLGIVPRAWHITMAVGDLERGKSRYIDYLDGRLLHEEENAASQTRSAFIAV
                     GTTTVIEVATPVNSDSLLAKDLEQNGDIIHSVTWGVRDVTKAAQFLRANGVGVEERTA
                     DTYLMHPDDTFGAVMYVSERRIPGDTRS"
     regulatory      1473..1593
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1787..>2620
                     /gene="thrC"
                     /locus_tag="PLX85_06220"
     CDS             1787..>2620
                     /gene="thrC"
                     /locus_tag="PLX85_06220"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRC62803.1"
                     /translation="MSFESALRCRECGREYPLRPIFSCEFCFGPLEVAYDLDGIREAI
                     SRDSIEAGPKTIWRYAPFLPCDPEYKVDLGTGYTPLIRADRLAKRLGLDTLWIKNDTV
                     NPTWSFKDRVVSIAIARARQFGFEAMACASTGNLANSVSAHAAAAGMPAYVFIPHDLE
                     EGKVVGSAIYDPTLVMVDGSYDDVNRLCTEVAGSRKWAFVNINVRPYYAEGSRTLAYE
                     VAEQLGWRAPGHVVAPMASGSLFTKIWKGFQEFHKAGLIEEPRTRMSGAQARGCSPIT
                     TA"
BASE COUNT          384 a         1041 c          763 g          432 t
ORIGIN      
        1 tgcgcacgcc gcagttcgtc tcgctgctcg tgctgctcgt ctccatcccc ggcatctggt
       61 acttcgcgag gcagccccgc cagcccatcg agccgctctc cgagccgcca ccagccgcac
      121 gccgcgcgac cgcccgccgt ggcggctgag ctttcgatgc ccgaggtcgt cctctatggc
      181 cagcccggct gccatctctg cgaggacgcc gaggccgccc tcgaggcgct gtgccgctcg
      241 cgacccttcg ccctgcgcgt cgtcgacatc aactccgacg acgacctcgc tcgccgctac
      301 gtcttcgaga tccccgtgat cgaggtcgat ggcgccgtcg tcactcgcgc cccggtcgac
      361 ctccgcgcgg tcaccgcggc cctcgacgct cccgcgagca ccgagggccg ccggtagctg
      421 ctgctagctc ctcgtgtccc ccgggatgcg gcgctcgctc acgtacatca cggcgccgaa
      481 cgtgtcgtcc gggtgcatca ggtacgtgtc cgcagtgcgc tcctcgacgc cgacgccgtt
      541 ggcgcgcagg aactgggccg ccttcgtcac gtcccgcaca ccccaggtca ccgagtgaat
      601 gatgtcgccg ttctgctcga ggtccttcgc gagcaggctg tcggaattca cgggcgtcgc
      661 cacttcgatc accgtggtcg tgcccaccgc gatgaacgcg ctccgggtct gcgaggccgc
      721 gttctcctcc tcgtgcagca gccgcccgtc gaggtagtcg atgtacctcg acttcccccg
      781 ttcgaggtcg ccgaccgcca ttgtgatgtg ccacgcccgc ggcacgatgc cgagcgggtg
      841 ctcgttcggc cagaagtcct cgtcgtagcc ggggatcgcc cccgttccga tgctcatccc
      901 ctcacgccga ggcatgagct cccactggca gcccgtgtcc cgcggatgcg tgaacgccgc
      961 cgccacgagg ttgtccgcgg acgttgccgc cccgccgccg tcggtgaaca gccgcacgcc
     1021 gccgtccttc agcttgccga acagctcacc aaacccgccg tcgatgtacc acgccaccga
     1081 gtgcagccgg ttcccgaacc gccgatggaa ccgtcccacc ggcatgtcct cggccccctc
     1141 gacccggtac gcgggcgcca tcggctccat gcagaagtcc gcgatgaacg ccagcgaggc
     1201 gtcccgcttc tccacctcgc tgtagtttcc gtgcatgctc cgcttcaccg cgaagatctt
     1261 gtcgtaccac tcatcgagcg gccccagctc gtcatacatg tggatgacgt ggaacatctc
     1321 gccgacctgg atcgccatcg ctcgcgctcc cttcgctgcc cccgttcgag gcgtgcgcat
     1381 actactccgc cggcgaatgc ctcgaaggtg ggcgtcgctc aattgacaga acgccccgtc
     1441 ccctcctagc ctcactccac gcacactcgt gcggttcatc gagagtggtg gagggcatcg
     1501 gccctgcgaa accacggcaa ccgagccgcc cctcgcccgg gcgggtacgg tgctaaatcc
     1561 gccccggccg cggcttgccg ggagagatga atcgaaggcg tccccacaca tgctgactga
     1621 ctcatccccc cgcctcagcc tcccggcgcg tgactcgatg cgcgcgatgg gcatccacca
     1681 gatggcgatg ccccggcccg accgctccta gcagcggctc gggccctcac cgcgcaccac
     1741 ccagcgttcg agcacaccgc ccgcgagggc gcgccggagt ccagcgatgt cattcgaatc
     1801 cgccctgcgc tgcagggagt gcggccgcga gtacccgctc cgccccatct tttcctgtga
     1861 gttctgcttt ggaccgctcg aggtcgctta cgacctcgac ggcatccgcg aggcgatctc
     1921 acgcgactcc atcgaggccg gccccaagac catctggcgc tacgccccct tccttccctg
     1981 cgaccccgag tacaaagtcg acctcggcac cggctatacg ccgctcatcc gcgccgatcg
     2041 cctggcgaag cgactcggcc tcgacaccct ctggatcaag aacgacaccg tgaaccccac
     2101 ctggtcgttc aaggaccgcg tggtgagcat cgccatcgcc cgcgcccggc agttcggctt
     2161 cgaggccatg gcgtgcgcca gcactggcaa cctcgccaac tccgtctccg cacacgccgc
     2221 cgccgccggc atgcccgcct acgtcttcat cccgcacgat ctcgaggagg gcaaggtcgt
     2281 cggcagcgcc atctacgacc ccaccctcgt gatggtcgat ggctcctacg acgacgtgaa
     2341 ccgcctctgc accgaagtcg ccggctcccg caagtgggcc ttcgtcaaca tcaacgtccg
     2401 cccctactac gccgagggct cccgcaccct cgcctacgag gtcgccgagc agctcggctg
     2461 gcgcgccccc ggccacgtgg ttgcgccgat ggcctcaggc tcgctgttca cgaagatctg
     2521 gaagggcttc caggagttcc acaaggcggg cctcatcgag gagccccgca cccgcatgtc
     2581 gggcgcgcag gcgcgcggct gctcgcccat caccaccgcc
//
LOCUS       DAORPG010000249         2613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4308_length_2613_cov_3.463253, whole genome shotgun sequence.
ACCESSION   DAORPG010000249 DAORPG010000000
VERSION     DAORPG010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4308_length_2613_cov_3.463253"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1040)
                     /locus_tag="PLX85_06225"
     CDS             complement(12..1040)
                     /locus_tag="PLX85_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRC62804.1"
                     /translation="MGRPRGFVSGAKTLWREMSSDDVNDFAAGLSYRFFLALFPFFIF
                     VTAVGGVVAQVAHVDNPATRLVNQFGDALPADARSVVQTQLEGVLGSQQPGLLSVGLI
                     GTIWAASGAVGALMKATNRMYDVEETRPLWKRTAVALGLTVLGGLFFVGAFILAVAGG
                     AIARAIVDAAGLGGVAEAGITVLGYVSAIVLLMVAIGFLYWATPNVRLPFKWVSPGAV
                     LFAVGWLGATLAFGLYVSNFGSYNATYGALGGVVILLVWFYLTSYILLAGAELNAVSA
                     QKTRPRDAEAPAPEPMRWERQTEQSEDLDRVAREVAFGQPAERRSPEPTSPMSDRAAG
                     AGTAESVG"
     gene            complement(1372..1740)
                     /locus_tag="PLX85_06230"
     CDS             complement(1372..1740)
                     /locus_tag="PLX85_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62805.1"
                     /translation="MIERRRTPIRLRTAALVLGVLAALVPAALFAAEVGQGGANPVVA
                     AQATQTATATLTATPTSTSTVTPTATATRTASPTAPQTGSAGFLNDSGSMGMALVLVG
                     LAVGVVAGGRMLSRHAGGEG"
     gene            2312..>2613
                     /locus_tag="PLX85_06235"
     CDS             2312..>2613
                     /locus_tag="PLX85_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62806.1"
                     /translation="MIDAEAVHTDEQIRELDSPIGPGFTDPADLNAIDVANAFYEDER
                     RAHEAALTAEVVRQEAEAEAVRQVEAQAAFDDGMTYVFGFNHEEGIRSFERAASRRS"
BASE COUNT          472 a          896 c          846 g          399 t
ORIGIN      
        1 gcggttcgca atcagcctac gctttcagcg gtccccgcgc cagcggcgcg gtcggacatg
       61 ggcgaggtgg gctccggact ccgtcgttcg gcgggctgac cgaaggccac ctcgcgcgcc
      121 acccggtcca ggtcctcgct ctgctcggtc tgccgctccc agcgcatcgg ctcgggcgct
      181 ggcgcctccg cgtcccgcgg ccgggtcttc tgagcggaga cggcgttcag ctccgcgccc
      241 gccagcagga tgtacgaggt caggtagaac cacaccagga ggatgaccac cccgcccagc
      301 gccccgtatg tcgcgttgta cgagccgaag ttggacacat agagcccgaa cgcgagcgtg
      361 gcgcccagcc atccgacggc gaacaggacg gctcccggtg agacccactt gaagggcaga
      421 cgcacgttcg gggtcgccca gtacaggaac ccgatcgcca ccatcaggag cacgatcgcg
      481 gacacgtacc cgagcaccgt gatcccggct tccgccacgc ctccgaggcc ggccgcgtcg
      541 acaatcgccc gcgcgatcgc accgccggcc accgccagga tgaatgcccc gacgaagaac
      601 agaccgccga ggacggtcag gccgagagcg acggcggtcc gcttccagag cggccgcgtc
      661 tcctcgacgt cgtacatgcg gttcgtcgcc ttcatgagcg cgcccacggc ccccgaggcc
      721 gcccagatcg tcccgatcag cccgaccgag agcagtcctg gctgctgcga ccccagcacc
      781 ccctcgagct gcgtctgcac cacggaacgt gcgtcagccg gcagagcgtc tccgaactgg
      841 ttcacgaggc gtgtcgcggg gttatcgaca tgcgccacct gtgcgaccac accgccaacg
      901 gcggtgacga aaatgaagaa cgggaacaac gcgaggaaga accggtacga cagcccggca
      961 gcgaagtcgt tgacatcgtc cgacgacatc tcgcgccaca gcgttttcgc cccggacacg
     1021 aaccctcgag gcctgcccat cgcgtcctcc ttcgtcgagt ctcacgatgc ggcctcgcga
     1081 ccgtgttacc gcccgagggc gaagctatca aggtggtatc tcgatgcgcc tcgcgccctc
     1141 tgtcgggtgg atgaccgagc acgaatcagc tgcctcgagt tcgcccgaat aacggttcaa
     1201 tcgtgagcga aaaagagctg tgacgcggga tccaccatcg gtcccgcgca acgcggtgag
     1261 gtcgcaaatg aaggcgtgtc actcgccgag tatgcgcacg cccgagcgtc aggccgggtt
     1321 tgcggctgga gtgatgcggg cggcccgttc gaggccgccc gcacgtccac gttacccctc
     1381 gccgcccgcg tgacggctca acatccggcc gcccgccacc acgccgaccg cgaggcccac
     1441 gaggaccagg gccatgccca tgctccccga atcattcagg aagccggcgc ttccggtctg
     1501 cggggccgtc gggctcgcgg tgcgcgtcgc ggtcgcggta ggagtcaccg tcgaggtcga
     1561 ggtgggcgtg gcggtgaggg tcgccgttgc cgtctgcgtc gcctgcgcgg ccacgacggg
     1621 gttcgcacca ccctgtccca cctcggcggc gaagagtgcc gccggcacga gggccgccag
     1681 cacaccgagt acgagggcgg cggtcctgag acgaattggg gttctacgtc gttcgatcat
     1741 gagtgctcct agattggtcg cgctccggaa tccccgggcg tcactcccga tggtagacag
     1801 cgccccaacc gctccgaccg cattcgccac gatgccggcg aggcccgcgg cgcatcgatt
     1861 tcatcacctt tgtatatgcg cacccgcttg ctccgaaaaa aatatgatga cttcgcaatc
     1921 ggactgaagt ggcagggatc gcgagggatg cgccgcgatc ggccgggatc ccctgctctc
     1981 tccggcatgt cctcactcgg ccagatcctc gcgggatatc cgaggacatc catcgattct
     2041 tctgcaagga ctcactacgg atcgatatgg atttggaaga tgtgtggaca gtgagggtcg
     2101 acgagccgtg tgagcagtag gcagagtgcc cccttgtgcg gaaagggggc acacattgag
     2161 acgaagaatc cggacatggc gagcattccg catcgggatc gtcgttgcat ccgcaacgct
     2221 ctcgatggcc atcgtcagcg gagcagcagc gtacatattc gcgggagaag aagggacacc
     2281 gacgaccgct gcatcggcca tcgatcgagg tgtgattgat gccgaagcag tccacaccga
     2341 cgagcagatc cgagaactcg attcgcccat tggcccgggc ttcaccgacc ccgccgacct
     2401 caacgccatc gatgtcgcca acgccttcta cgaggatgag cgtcgggccc acgaggcggc
     2461 cctgacggcc gaggtcgtgc ggcaggaggc cgaagcggag gccgtccgcc aggttgaggc
     2521 gcaggcggcc ttcgacgacg gcatgaccta cgttttcggt ttcaaccacg aggaaggcat
     2581 ccgctcgttc gagcgggcgg cgagccgcag gtc
//
LOCUS       DAORPG010000250         2609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4316_length_2609_cov_3.747847, whole genome shotgun sequence.
ACCESSION   DAORPG010000250 DAORPG010000000
VERSION     DAORPG010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4316_length_2609_cov_3.747847"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="PLX85_06240"
     CDS             <1..1281
                     /locus_tag="PLX85_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRC62807.1"
                     /translation="RLDAAGVGRILEDSAPRVVVAHASTVPGDLPPATTALVVVGGKD
                     AAIDYEHVLAGASDAPLDEAVAPDDLCMLPYTSGTTSGPKGVMLTHANVTWNVVNVLS
                     GADFRAGDVTLAVTPFFRTGGTGVNVLPTMFLGGTVVIPEDPDPGALLDLMERHEVTV
                     GFGNPDILEGLTRTARWKDADLTRLRFIITGGAPVPERLIRACLERNVLLLQGYGLSE
                     AAPVALLLDSESALTKGGSAGKPPLLMSTRIVRPDGNECEVGETGELLVAGPNVMRGY
                     WNRPEATERALDADGWLHTGDAARADDDGYISIVDRLGDRFTARGVVVYPGDVERLLL
                     GHPAVADAGVTGIEVGGETVGAAFVVLTAPDAAAPEELLAHWQSSLRPHQLPEVLHIV
                     PSLPRSSVGKLLRHELAALVGDEPHASAGTPLGT"
     gene            complement(1248..2312)
                     /locus_tag="PLX85_06245"
     CDS             complement(1248..2312)
                     /locus_tag="PLX85_06245"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRC62808.1"
                     /translation="MALQPHLDLESLRSLLADLEERRQRIEALDVPPLLARWLRRITE
                     ARGAHMSTRIEGNPMTEQEVREVFARPERRTEVAEIENLNYRDAVQFASQVAGDTSMA
                     SGPDAGLIRALHFLILRDVDRYGTAGQYRTKQNAVRHAGETIYLPPSPADVGPLMDDL
                     VTWIAGAGRQGTHPLVVAAVAHAEFINIHPFDDGNGRTGRALTSLLMFRGGWWLRGFV
                     SAEQVFGSDVQAYYSALRAFGAKYPGQQVEFTEWARWFLEGLTRQADARLEEASQQLG
                     REALASVGDTGQRERLATALTYASLFGTVTSREYAAEAGVSAATAVSDLNWLVASGHL
                     KRLGSGRATRYVPRGVPAEA"
     gene            complement(2374..2514)
                     /locus_tag="PLX85_06250"
     CDS             complement(2374..2514)
                     /locus_tag="PLX85_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62809.1"
                     /translation="MKNIPRIRDVYREYELGRIPFDDVLAASERIIAQWEVREAESKA
                     RS"
BASE COUNT          398 a          925 c          871 g          415 t
ORIGIN      
        1 cgcctcgacg ccgccggggt gggccgcatc ctcgaggact cagcgccccg cgtcgtcgtg
       61 gcgcacgcct cgacggtacc gggcgatctg cctccggcca ccaccgcgct ggtggtcgtg
      121 ggcggcaagg atgcggcgat cgattacgag cacgtgctgg cgggagcctc ggacgcgccg
      181 ctcgacgagg cggtcgcgcc cgacgacctc tgtatgctgc cgtacacctc gggcacgacg
      241 agcgggccga agggcgtgat gctgacgcac gcaaacgtga cctggaatgt cgtcaatgtc
      301 ctctccggcg cggacttcag agccggcgat gtcacgctcg ccgtgacgcc cttcttccgg
      361 accggcggga cgggcgtcaa tgtcctcccg acgatgttcc tgggcggcac ggttgtgatc
      421 cccgaggacc cagaccccgg tgcgctgctc gacctcatgg agcgacacga ggtcacggtg
      481 ggcttcggca accccgacat cctcgagggt ctgacacgca cggctcgctg gaaggacgcc
      541 gacctcaccc gtctgcgctt catcatcacg ggcggagcac ccgtcccgga acgcctcatc
      601 cgcgcctgcc tcgagcgtaa cgtcctgttg ctccaggggt acggactgtc cgaggccgcc
      661 ccggtcgccc tgctgctcga ctccgagagc gccctcacga aggggggctc ggcgggcaag
      721 ccgccgctgc tgatgagcac gcgcatcgtc cgtcccgatg gcaacgaatg cgaggtgggc
      781 gagaccggcg agctgttggt cgccggccca aacgtcatgc gcggctactg gaaccgcccc
      841 gaggcgacgg aacgagccct cgacgccgac ggttggctgc acaccggtga cgcggcccgt
      901 gccgacgacg acgggtacat ctcgatcgtc gaccggctcg gcgaccgctt caccgcccgc
      961 ggggtggttg tgtatccagg tgacgtggag cggctgctcc tcgggcaccc ggccgtcgcc
     1021 gacgccggcg tgaccggcat cgaggtgggc ggcgagaccg tcggcgccgc ttttgtcgtg
     1081 ctgaccgctc cggacgcggc agcacccgag gagttgctcg cccattggca gtcttcgtta
     1141 cgcccgcacc agctccccga agtgctccac atcgtgccgt cactcccgcg cagctccgta
     1201 ggcaaactgc tacgccacga actcgccgcg ctcgtcggcg acgagcctca cgcctcggcg
     1261 ggcacgcccc tcgggacgta gcgcgtcgcg cggccactgc ccagccgttt gaggtgccct
     1321 gaggccacga gccagttgag gtcggagacg gccgtcgcgg ccgagactcc ggcctcggcc
     1381 gcgtactctc ggctggtgac tgtgccgaac agcgaggcgt atgtgagcgc cgtcgccagc
     1441 cgctcacgct gccccgtgtc gccgaccgag gcgagcgctt cgcgaccgag ctgctgcgag
     1501 gcctcctcaa gccgtgcatc cgcctgcctc gtcagcccct cgaggaacca gcgcgcccac
     1561 tctgtgaact cgacctgctg cccggggtac ttggcgccga atgcccgcag ggcgctgtag
     1621 tacgcctgca cgtcgcttcc gaatacctgc tcagcgctca cgaagccacg cagccaccag
     1681 ccgccccgga acatcagcag cgaggtcagc gcgcgtcccg tccggccgtt gccgtcatcg
     1741 aaggggtgaa tgttgatgaa ctcggcgtgc gcgaccgccg cgacgacgag cgggtgcgtg
     1801 ccctggcggc ccgctcccgc gatccatgtc acgaggtcgt ccatcagcgg cccgacatca
     1861 gcaggcgacg ggggcaggta gatcgtctcc cccgcgtggc gcaccgcgtt ctgcttcgtg
     1921 cggtactgcc ccgccgtccc gtagcggtcg acgtcacgca ggatgaggaa atgtagcgcg
     1981 cggatcagcc ccgcatccgg gcccgacgcc atcgaggtgt cccccgccac ctgcgaggcg
     2041 aactgcacgg cgtcccggta gttgaggttc tcgatctccg cgacctcggt gcggcgctcc
     2101 gggcgcgcga acacctcgcg cacttcctgc tcggtcatcg ggttgccttc gatacgggtc
     2161 gacatgtggg cgccgcgcgc ctcggtgata cgcctcagcc agcgggcgag caatggcggg
     2221 acgtcgagcg cctcgatgcg ctgtcgccgc tcctcgaggt ccgcgagcag cgaccggagc
     2281 gattcgaggt cgagatgcgg ctggagggcc atagttcatc gattatagga acaatcaacc
     2341 aactcagtcg attgttcgaa tagtcgaagg agtctaggaa cgagccttgg actcggcttc
     2401 gcgcacctcc cattgggcaa tgatgcgttc cgatgcggca agtacgtcat cgaacggaat
     2461 acgtccgagc tcgtactcgc ggtagacatc gcggattcga gggatgttct tcacccgtcg
     2521 ctcctctccc aggacaggcg gatgcctggc tcgtagcaga tggcccagaa ccagtgccgc
     2581 ggatcgaggt cgtgcaccgg tccgggctc
//
LOCUS       DAORPG010000251         2609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4319_length_2609_cov_2.923258, whole genome shotgun sequence.
ACCESSION   DAORPG010000251 DAORPG010000000
VERSION     DAORPG010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4319_length_2609_cov_2.923258"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PLX85_06255"
     CDS             <1..752
                     /locus_tag="PLX85_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62810.1"
                     /translation="RSAATRARDGAVAAYTSPRTRQARANVALYTRRAAAGAADATRR
                     GSTYVRQQYERDARQTLIVGGVVVAVIVLVLVLLVSALSGGGDDPTASSGNAGAVQPG
                     IGTPAASGTNGGTVASARSSTATAAAAATLPGNPYSETAVLAAMQARGLTPTLSTDAF
                     ACDNPGTTPKSYRVSTNGGEQRVVLLIYPDAAAFGRDWVVGAGRPQYRNGACGAGAAV
                     VYFNANVMIVFPQTTNAGVQSQISDAFLTLP"
     gene            complement(756..1841)
                     /locus_tag="PLX85_06260"
     CDS             complement(756..1841)
                     /locus_tag="PLX85_06260"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRC62811.1"
                     /translation="MRIGRRAFLQSTLAALTGAAVACASDASGDVRIERRATSSVVAA
                     SPGATAVPTAAVDPAIRIGAVGDLMFARDITTLMLQHGAGYPFERVRPLFEGLDFLVG
                     NMEGTFTTRGLALTKAYTFRTPPELATGLTAAGFDLVALANNHAFDFGSVSLEDTLAA
                     LQRAGVVSVGAGTTEAAARAGYVAEVRGKRVAALSYSGVDESGFATPGGPGVARATVE
                     AVREDVQRLAPLADHVLVFMHAGVEYTRAPSDLQRAIAYAAIESGADVVIGSHPHVLQ
                     GWERHHDGLILYSLGNFVFDLDTDDLSNFGGPPFETAVAVLTLRPDAPPAVEFRPAYI
                     DPIEDRPRPATSDEAARVMAALREIPR"
     gene            1943..>2609
                     /locus_tag="PLX85_06265"
     CDS             1943..>2609
                     /locus_tag="PLX85_06265"
                     /inference="COORDINATES: protein motif:HMM:NF022734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HRC62812.1"
                     /translation="MIDFGIVDAVDAEAIGEPGQRTFRLRARSGANYAALWLEKEQLA
                     TLGRAISQVLAERSAHRGDPVGEAAAIGSFVTHPDVDMQVVRLGLDFDADTDRLALLA
                     DDQAGLERGDTPTFRMEIRSCNDSKMGRTRPASENAGTMTPLGAWRRFVPGVNRGLAA
                     IRGWARGSAGQNWGGSRDDGDGRACLAERRSGAKAASACPGPDCRRRPGRFQRPPAPA
                     SALH"
BASE COUNT          431 a          914 c          889 g          375 t
ORIGIN      
        1 cacgcagcgc ggccacccgt gcccgcgacg gcgcggtcgc cgcatacacc tcgccacgca
       61 cgcgccaggc tcgagcgaac gtcgcgctgt acaccaggcg tgccgcggct ggagcggcgg
      121 acgcgacgcg acgtggttct acatacgtca gacagcaata cgagcgtgac gcgcgtcaga
      181 cgctgatcgt cggcggcgtg gtcgtggccg tgatcgtgct cgtactcgtc ctgctcgtct
      241 ccgcgctcag cggcggaggc gacgatccga cagccagcag cggcaacgcg ggagccgtgc
      301 agcctggcat cgggacgccc gccgcgagtg gcacgaacgg cggcacggtc gcgtccgccc
      361 gttcatcgac ggcgaccgcc gcggcggcgg caacgctgcc tggcaacccg tacagcgaga
      421 ccgcggtgct ggcggcgatg caggcacgag gactgacacc caccctgagc accgacgcct
      481 tcgcctgcga caatccgggc accacgccga agagctaccg ggtaagcacg aacggcgggg
      541 agcaacgcgt cgtcctgctc atctaccccg acgccgcggc gttcgggcgt gactgggtcg
      601 tcggagccgg ccggccccag taccgcaatg gcgcctgcgg cgcgggcgcg gccgtcgtct
      661 acttcaacgc caatgtcatg atcgtgttcc cgcagacgac caacgcgggt gtccagtcgc
      721 agatttccga cgccttcctc acgctcccct gacccctacc tcgggatctc ccgcaacgcc
      781 gccatgaccc ttgccgcctc atccgaggtc gccggacgcg ggcggtcctc gatcggatcg
      841 atataggccg gacggaactc cactgccggt ggtgcgtccg gtcgcagcgt gaggacggcg
      901 acggccgtct cgaagggggg gccgccgaag ttgctgagat cgtccgtgtc caggtcgaac
      961 acgaaattgc cgaggctgta caggatcagg ccgtcgtggt ggcgctccca cccctgcagg
     1021 acgtgcggat gcgacccgat gacgacatcc gctcccgact cgatggcggc atacgcaatc
     1081 gcgcgctgca ggtcggacgg cgctcgggtg tactcgaccc cggcgtgcat gaacacgagc
     1141 acatggtcgg ccagcggtgc gagtcgttgg acgtcctcgc gcaccgcttc gacagtcgca
     1201 cgcgcgacgc cagggccgcc gggcgtcgcg aatccggatt cgtcaacgcc gctgtagctg
     1261 agggcggcaa cgcgtttgcc gcgtacctcc gcgacatacc ctgcacgcgc cgccgcttcg
     1321 gtagtaccgg ccccgaccga cacgaccccc gcccgctgca acgcagccag cgtgtcctcg
     1381 aggctcacac tcccgaaatc gaaggcgtgg ttgttggcca gcgcgacgag gtcgaagccc
     1441 gccgcggtaa gcccggtcgc aagttctggc ggagtgcgaa acgtgtacgc cttcgtgagg
     1501 gccaacccgc gagtggtgaa agtgccctcc atgttcccga cgaggaagtc caacccctcg
     1561 aacaacggcc gtacccgctc gaagggatag ccggcgccgt gctgcagcat cagcgtcgtg
     1621 atgtcgcgcg cgaacatgag gtcgcccacg gccccgatgc ggatcgctgg atcgacggcg
     1681 gccgttggca cggcggtcgc gcccggtgag gcggcgacga ctgaggacgt cgcccgtcgc
     1741 tcgatgcgca cgtcccccga ggcgtccgag gcgcaggcaa ccgccgcgcc cgtgagcgct
     1801 gcaagcgtgg actgcagaaa ggcgcgacgg ccgatacgca tgcctgagat agtagaacga
     1861 ggtcgaacac agcctctcgt cgtccgcggt tgaccccgac gcagggcctc cctacgctcc
     1921 gcccacctga ggaggtgcgc cgttgatcga ctttggcatc gtcgacgccg tggacgcgga
     1981 agccatcggc gaaccggggc agcggacctt ccggctacgg gcgcgctcgg gcgcgaacta
     2041 tgcggcgctc tggctggaaa aagagcaact ggccaccctt ggccgtgcca tctcgcaggt
     2101 gctggctgag cgctccgccc atcgaggcga tcccgtgggc gaggccgccg ccatcggctc
     2161 gttcgtcacc caccccgacg tcgacatgca ggtcgtccgt ctgggcctcg acttcgacgc
     2221 cgataccgac cgcctcgcat tgctcgcgga cgaccaggcg ggcctcgaac ggggcgacac
     2281 gcccaccttc cgcatggaga tccggtcatg caatgactcc aaaatgggcc ggaccaggcc
     2341 ggcgtctgaa aacgccggga ccatgacgcc tttgggagcg tggcgcaggt tcgtacccgg
     2401 tgttaacaga ggcttggcgg caatacgggg ttgggcgcga gggagcgcag gccagaattg
     2461 gggcgggtcg cgagatgacg gagacggccg ggcctgcctt gcggagcgcc gtagcggagc
     2521 gaaagcagct tctgcttgcc ctggaccgga ttgtcgccgt cggcctggcc gattccagcg
     2581 accgccagcg ccagcttctg cgctacatc
//
LOCUS       DAORPG010000252         2605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4337_length_2605_cov_2.560000, whole genome shotgun sequence.
ACCESSION   DAORPG010000252 DAORPG010000000
VERSION     DAORPG010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4337_length_2605_cov_2.560000"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2237)
                     /locus_tag="PLX85_06270"
     CDS             complement(<1..2237)
                     /locus_tag="PLX85_06270"
                     /inference="COORDINATES: protein motif:HMM:NF020243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC62813.1"
                     /translation="MLVSLVSQRTGYPAEMLDLDLDLEADLSIDSIKRVEIVGAMLEQ
                     LVHGDSPALNETPDELVRLKTLRAISTALEQTFGGVAAAPAPADVQGASVASVPAAAG
                     TEGASTPSDLTTMLVTIVSERTGYPPEMLDLDLDLEADLSIDSIKRVEIVGAVLDGLA
                     SGGMDGFAETPDELVRLKTLRGIVAALETVFAPAAAAPLTNGHRPALSTNGHATAVAA
                     PPTITTAGPALMATLVQIVSERTGYPPEMLGADLDLEADLSIDSIKRIEILGALSEVA
                     GLAATELPDEVVRIKTLRGIVAAFSQQPSTGGEADPRATAGLAAASQPALTDSMPGER
                     PERYVLRFVATAGPPDEVPLTGLAVSVAAPDGPLQSALAEEIARLGGELAAGDATGNA
                     VVDLSALAPDWSPRQVYSTFERLRAALLEGAGRVIVATAEAPLLMGAADATRGAAGGV
                     AGLVKSFAREWPERSIRVMHVEPGIEPGQLAAFVCAELGDQHGADEVVYTGGVRRVRR
                     VVQRDLEPNVSPAVQLDAQSVILVVGGARGITSRVAVALANRYHCTLYLAGRTPRVAD
                     PLAGQLGSARDLAALRKALIELSAGEIRTIDARARSILAQREIVDTLAAVQAAGGTAS
                     YEELDARDAAALGGLIETIRRRHGRLDGIIHAAGLIEDRLVTDKTAESFERVYSTKVE
                     PALALASGLAGGAQFVALFSSIAATFGGRGQADYAAANDVLDKVALAMNGQASARWLR
                     STGGHG"
BASE COUNT          368 a          968 c          864 g          405 t
ORIGIN      
        1 ccatggcccc cagtcgaccg caaccagcgc gcactggcct gcccgttcat cgcgagggcg
       61 actttgtcga gcacgtcgtt cgccgcggcg tagtctgcct gcccgcggcc cccgaacgtt
      121 gccgcgatgc ttgagaacag cgccacgaat tgggcgccac ccgcgagccc gctcgcgagc
      181 gcgagtgctg gttcgacctt cgtcgaatac acccgctcga acgactccgc cgtcttgtct
      241 gtcacgagcc ggtcctcgat cagaccggcc gcgtggatga tcccgtccaa cctgccatgc
      301 cgcctgcgga tcgtctcgat tagcccgccg agtgctgcgg cgtcgcgtgc gtcgagttcc
      361 tcgtacgagg ccgtcccgcc cgccgcctgc acggccgcca gcgtatcgac gatctcccgc
      421 tgcgccagga tcgaccgggc ccgggcgtcg atggtccgga tctcacccgc actcagctcg
      481 atcaacgcct tgcgcagcgc tgcgaggtcc ctcgcggatc caagttgccc cgcaagcggg
      541 tctgctacac gcggggtgcg tcccgcgagg tacagcgtgc agtggtagcg gttcgccagc
      601 gccaccgcga ctcggctcgt gatccctcgg gcgccgccga ccaccaggat gacggactgc
      661 gcatcaagct ggacggccgg cgagacgttc ggttcaaggt ctcgctgcac gactcgccga
      721 acgcgccgga cgccgcccgt gtacaccacc tcgtccgcgc cgtgctggtc gcccagctca
      781 gcgcacacaa acgcggccag ttgccccggc tcgatgccgg gctcgacatg catcacgcgg
      841 atcgaccgct cgggccactc ccgcgcgaag ctcttcacga ggcctgccac gccgccggcc
      901 gcgccgcggg ttgcgtctgc ggcccccatt agcagcggcg cctcggccgt cgccacgatc
      961 acacggcccg cgccctcgag cagcgccgcc ctcagccgct cgaaggtgga gtagacctgc
     1021 cgagggctcc agtccggcgc gagcgccgac aggtcgacca ctgcattccc cgtcgcgtca
     1081 cccgctgcga gctcgccgcc gagccgcgcg atctcttcag ccagcgccga ctgcagcggc
     1141 ccatcgggcg cggccaccga gacagccagc ccggttaatg gcacttcgtc cggcggaccg
     1201 gcggtcgcga caaagcgcaa cacatagcgc tccggccgct cccccggcat cgagtcagtg
     1261 agggcgggtt gcgaggctgc cgccaacccc gcggtcgccc tcggatccgc ctcgccgccc
     1321 gtcgagggct gctggctgaa cgccgccacg atgccgcgca acgtcttgat ccgcaccact
     1381 tcgtcgggaa gctccgtcgc cgccagcccg gccacctcag agagcgctcc caggatctcg
     1441 atgcgcttga tcgagtcgat gctcaggtcc gcctccaggt cgaggtccgc gcccagcatc
     1501 tccggggggt agcccgtgcg ctcgctgacg atctgcacca gcgtcgccat cagggcgggg
     1561 cccgccgttg tgatcgtcgg cggagccgcg acggcggtcg cgtgcccgtt cgtggatagc
     1621 gcggggcgat ggccgttcgt aagcggcgcg gcggctgccg gcgcgaacac cgtctcgagg
     1681 gcggcgacga tcccacgcag ggtcttcagt cgtaccagct cgtccggtgt ctccgcgaag
     1741 ccgtccatcc cgccgctcgc gagcccgtcg agtaccgcgc ccacgatctc gacacgcttg
     1801 atggagtcga tgctgaggtc cgcctccagg tcgagatcga ggtcgagcat ctccggcggg
     1861 tacccggtcc gctcgctcac gatcgtgacc agcatcgtgg tcaggtcgga cggcgtggat
     1921 gctccctcgg ttccggccgc tgccggaacc gacgcgacgg aagcgccctg gacgtccgct
     1981 ggcgcgggag ctgcggccac accaccaaag gtctgctcca gtgcggtgct gatcgcgcgt
     2041 agcgtcttga ggcgcacgag ttcgtcgggt gtctcgttca gcgcgggcga atcgccgtgg
     2101 acgagctgct cgagcatcgc cccgacgatc tcgactcgct tgatggagtc gatgctgagg
     2161 tccgcctcga ggtcgagatc gaggtccagc atctccgcgg ggtatcccgt ccgctggctt
     2221 accaggctga cgagcatcgt ttgaatcggg ggccgttgcg aggccgccac cgcaggctcg
     2281 gccaccgcca cgaggccgac tggctcaacc ctcgcggcga ctgggacggc gggagcatcc
     2341 ggggcgattc cattcggacc atgtccgttt cgaggaagct cgctcgtgcg agggggtgcg
     2401 ctgcctgtga ccgcatccgc gtgtggtgac gtcggagcgt ggccattcag ggcgcgcgcc
     2461 ggctcggatg gggcgctccc ctgtccgagg aatgccatca ccgcctgatg ctgcgcatcc
     2521 gccagctcgc gcacactgcg caggtactcg atcaacacgg cctgccgcgg atcgccgtca
     2581 gcgagtgcgc gcggcatcga caggc
//
LOCUS       DAORPG010000253         2597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4359_length_2597_cov_3.113297, whole genome shotgun sequence.
ACCESSION   DAORPG010000253 DAORPG010000000
VERSION     DAORPG010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4359_length_2597_cov_3.113297"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..945)
                     /locus_tag="PLX85_06275"
     CDS             complement(115..945)
                     /locus_tag="PLX85_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRC62814.1"
                     /translation="MVTHTQLDPLTLSERVRRHVVTMVYEAQSGHIGGSLSATDIMSV
                     LYTRILRVDPQRPDWADRDRFILSKGHCTPVQYATLAEMGYFPVEELRTFRKKGTRLQ
                     GHSILGKPPGVENSAGALGMGLSFGLGIALGRDFRGQDFHVYVLMGDGEQNEGQVWEA
                     AMAVANYNLERIVAIVDRNHIQNDDFTANQMAIDPIDEKYRAFGWTVRSGIDGHNLKD
                     VEEALTWARDHRGGPACLVFETVKGKGVSIFENNPGFHGAAPNAEQYAQAMRELGGEG
                     "
     gene            complement(1025..1396)
                     /locus_tag="PLX85_06280"
     CDS             complement(1025..1396)
                     /locus_tag="PLX85_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62815.1"
                     /translation="MPVPLRVSSLLITDALAGASDLYRQLGFEPVPTDDEACIGFTAG
                     TTGVILTSREFAASCWGEPVAAELAGRVVPYVYLQHLSDSDDVGDTIFEGWTQYGTHD
                     RIVGTAFGPMVLVERAHPLTL"
     gene            1625..1879
                     /locus_tag="PLX85_06285"
     CDS             1625..1879
                     /locus_tag="PLX85_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HRC62816.1"
                     /translation="MRTTLTLDDDVAKLLEDARRKTGASLRQLVNEALRSSLEGRKRA
                     TPQRYSTKAVSLGRPHLEHMDDVADALAAVEGEGFQPTGR"
     gene            complement(2159..>2597)
                     /locus_tag="PLX85_06290"
     CDS             complement(2159..>2597)
                     /locus_tag="PLX85_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HRC62817.1"
                     /translation="ELSRELGERGVLLHTDERARPLCGGADTLPVEPEDYDREWLSLD
                     CSVRVVDSLDDALKHIEDHGSGHSEAIVTNSYAASRRFLAEADAAAVYVNASTQFTDG
                     AQFGLGAEIGISTQKMHARGPMALRELTSYKWIIFGEGQTRPL"
BASE COUNT          406 a          810 c          942 g          439 t
ORIGIN      
        1 gccgaggtcg gcgtcgacga cggcgaggtc catcccctcc tggacgaggc ggatgagcgt
       61 cgggccgagg gcctcgcggg tagcggcgct ggcgggagcg gtcatggtca tggcttagcc
      121 ctccccgccg agctcacgca tggcctgtgc gtactgctcg gcgttcggcg cggcgccgtg
      181 gaagccgggg ttgttctcga agatggagac gcccttaccc ttcacggtct cgaagacgag
      241 gcaggccggg ccgcctcgat ggtcgcgggc ccaggtcagg gcttcctcga cgtccttgag
      301 gttgtggccg tcgatgccgg aacgcaccgt ccagccgaag gcgcggtact tttcgtcgat
      361 ggggtcgatc gccatctggt tcgcggtgaa gtcgtcgttc tggatgtggt tgcggtcgac
      421 gatcgcgacg atgcgctcga ggttgtagtt ggcgacggcc atcgcggctt cccacacctg
      481 gccctcgttc tgctcgccgt cgcccatcag gacgtagacg tggaagtcct gccctcggaa
      541 gtcgcgcccg agggcgatgc cgaggccgaa cgagagcccc atgcccagcg ccccggccga
      601 gttctcgact ccgggcggct tgccaaggat ggagtggccc tgcagccggg tgcctttctt
      661 gcggaaggtg cgcagctcct cgaccgggaa gtagcccatc tcggcgaggg tggcgtactg
      721 gacgggggtg cagtggccct tgctcaggat gaagcggtcg cggtccgccc agtcggggcg
      781 ctgcgggtcg acgcgcagga tgcgcgtata gaggacagac atgatgtcgg tggcggagag
      841 cgaccctccg atgtgccccg actgcgcctc gtagaccatc gtgacgacgt gccgccggac
      901 ccgctcagac agcgtgagcg ggtcgagctg agtgtgagtg accaccgtga gacccccgcg
      961 tttggaatga acctccagta tacggagggc cgttcgtggg ggtctatgaa cgtagtatcg
     1021 agtgttagag cgtgagggga tgggctcgtt cgacgagcac catcgggccg aatgccgttc
     1081 ccacgatccg gtcgtgcgtg ccgtactgcg tccagccttc aaagatcgtg tcgccgacgt
     1141 cgtcggagtc gctcagatgc tggaggtaca cgtagggcac aactcgaccc gcgagctcgg
     1201 cggctactgg ctcgccccag caacttgccg caaattctcg gcttgtgagg atgacgccgg
     1261 tggtgcccgc ggtgaagccg atacatgcct catcgtcggt tggtaccggt tcaaagccca
     1321 gttggcgata cagatcgctt gcgcccgcca gtgcatctgt gatcagaagc gaggagacgc
     1381 gcaggggaac cggcatcgta aagacctcct tggggagccc gaagaacgta gcacgaacag
     1441 acgttctgtg cgccggcgcg ccagtatcca cggccggccg tactgccgga tgccatgcct
     1501 cgaccgcgac gagcccgggt gcgacgtagg ctccgacccg gacgcacttc ccacaggcgt
     1561 caccgcgcga gagtctgcat cgcgtacggg caagctggcc tcggctgcgg gtatgctgcg
     1621 atgcatgcgg acgactctga cgctcgatga cgatgtggcg aagctcctcg aggacgcgcg
     1681 ccggaagact ggtgcgagtc tgcgccagct ggtgaacgag gcgctgcgat cgagcctcga
     1741 ggggcggaag cgggccaccc cgcagcgtta ctcgacgaag gcggtgtcgc tcgggcggcc
     1801 acacctcgag cacatggacg atgtggctga tgcgctggcc gcggttgaag gagagggctt
     1861 ccagccgacc gggcggtgac ggcgcccgac tacgccctca ttgactcggg ggccaggcag
     1921 ccgtgttcag acctcgacgg cggtgcattc aggcgccgtg acttccgagg ggacgggctc
     1981 gccagcgacg gtcaacgtac cgacgtgcag cgagatcgcc tcgcgcgcgt tcgcgagggc
     2041 gtcctcgagg gtgtggcttc ggtaacccag ccgggcggca cggggacgag gacggtgtaa
     2101 ccgccttctt cggtatcggg gaggaggggc accgagtagc ggcgcgtctc ctatctcctt
     2161 acagcggccg cgtctggccc tcgccgaaga tgatccactt gtacgaggtg agttcgcgca
     2221 gggccatcgg gccgcgggcg tgcattttct gggtggagat gccgatctcc gcgcccaggc
     2281 cgaactgcgc tccgtccgtg aactgcgtcg aggcgttcac gtagaccgcc gcggcgtcgg
     2341 cttccgcgag gaagcgccgg gaggccgcgt atgagttcgt cacgatcgcc tcggagtggc
     2401 cgctgccgtg gtcctcgatg tgcttgaggg cgtcgtcgag ggagtcgacc acgcgcacgg
     2461 agcagtcgag cgagagccac tcgcggtcgt agtcctcggg ctccacgggc agtgtgtccg
     2521 cgccgccgca aagcgggcgc gcgcgctcgt cagtgtgcag caggacgccg cgctcgccga
     2581 gctcgcgtga gagctcg
//
LOCUS       DAORPG010000254         2583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4404_length_2583_cov_3.144778, whole genome shotgun sequence.
ACCESSION   DAORPG010000254 DAORPG010000000
VERSION     DAORPG010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4404_length_2583_cov_3.144778"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PLX85_06295"
     CDS             complement(<1..393)
                     /locus_tag="PLX85_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRC62818.1"
                     /translation="MTPLATLDWRLRAAITAAILLVILGLFVQREFTGADGSGASDAG
                     SAGLVDEAPATIGAPAPDFVLRTVGGDTVRLSDYRGKTVVLNFWASWCVPCRREMSEF
                     EAMYRDRLGRSDFVVIAVDYRPLDSVSEV"
     gene            475..1275
                     /locus_tag="PLX85_06300"
     CDS             475..1275
                     /locus_tag="PLX85_06300"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HRC62819.1"
                     /translation="MLPAPDTDAIDEAAALLVGAQHAVALTGAGISKESGIPTFRGKD
                     GLWTVRGEPPLNQFQEFHRDPKAWWVRRLEQRANPDEFALSLSASEPNDAHYALAELE
                     SIGVLRHVITQNVDNLHRRAGQQSITEIHGNMRWMRCTDCHTRWPEDKVDVNLEDLPP
                     RCPEPGCSGIVKGDGVMFGEPIPPDALERCYAEAAMADVFLLVGTSAVVYPAAMFPQL
                     AHQRGASLIEIDPEPTALSELARVALRGPAGEMLPLLLRAVRERIERR"
     gene            1272..2204
                     /locus_tag="PLX85_06305"
     CDS             1272..2204
                     /locus_tag="PLX85_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62820.1"
                     /translation="MSPRLNLALLVGAEDGLWSMLPGDEAKLVIETRSVRSLDVRGDL
                     AIAGSAEGVWLHRKGAWKQVLEGDVRCVRIGPEGTLVAGVATSTVLASDDEGASWHEW
                     ETLQNLVRYHGQRMGTRAAGAPVSGIAFASGIIVAFSGIGAFLTLDRGRTWALHSEGL
                     DRRVHGLWDHPERAERLYAAAPSGFYRTEDGGYSWVQSLHGLDRSWACDMAVLPGVPD
                     TLVLSAARAGDGEGSALFHSADGAISWTRLLLGENDEWSRPPLVTSLTSPVDALFVLA
                     GHEVWGSHDRGERWIPMADGLPLGNTFLAAVGEW"
     gene            2318..>2583
                     /locus_tag="PLX85_06310"
     CDS             2318..>2583
                     /locus_tag="PLX85_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRC62821.1"
                     /translation="MANRTQDQMVEANGLRLHVHEWGDSRTKHALVLLHDYAETTAAW
                     EEVAVDLSREYRVIAIDQRGHGRSDRAGDRDYSRASQVEDIAAV"
BASE COUNT          416 a          855 c          908 g          404 t
ORIGIN      
        1 cacctcggaa acactatcca gcggacggta gtcgaccgcg atcactacga agtcgctgcg
       61 tcccaggcgg tcgcgataca tggcctcgaa ctcactcatc tcccggcggc acgggacgca
      121 ccagctggcc cagaagttca ggacgaccgt tttgcctcgg tagtccgaga ggcggaccgt
      181 atcgcccccg acggtgcgga ggacgaagtc cggcgcggga gcgccgatgg tcgccggcgc
      241 ctcgtcgacg agtcccgccg agccggcgtc cgaggcgcca cttccgtcgg cgccggtgaa
      301 ctcccgctgg acaaagagtc cgaggatcac gagcaggatc gccgccgtga tggccgctcg
      361 gaggcgccag tcgagcgtcg cgaggggggt cacggatcta ttgtgtcgtc ggggggagcg
      421 ctggcgaaac tcagccgcgc ttgcaactcg atcggcggtc gctaggatgt ggtcgtgctg
      481 ccggcgcccg acaccgatgc gatcgatgaa gccgcggcgc tcctcgtcgg cgcgcagcat
      541 gcggtcgcgc tgaccggagc gggcatctcg aaggaatcag gcatcccgac gttccgcggc
      601 aaggatggtc tctggaccgt gcggggtgaa ccgccgctca accagttcca ggagttccac
      661 cgggatccga aggcgtggtg ggtccgccgc ctcgaacagc gggccaatcc cgacgagttc
      721 gcgctctcgc tctccgcgag tgaaccaaac gacgcgcact acgccctggc ggagctcgag
      781 tcgatcggcg tcctgcgtca tgtgatcacc cagaacgtgg acaacctgca ccggcgagcg
      841 gggcaacagt ccattaccga gatccacggc aatatgcgct ggatgcggtg caccgattgc
      901 cacacccgct ggccggagga caaggtcgac gtgaacctcg aggacttgcc cccgcgctgc
      961 ccggagccag ggtgctcggg catcgtgaaa ggcgatgggg tcatgtttgg cgagccaatc
     1021 ccgccggacg ccctcgagcg ctgctacgca gaggcggcca tggctgacgt gtttctgctc
     1081 gtgggaactt cggcagtcgt ctacccggcg gcgatgtttc cgcagctcgc ccatcaacga
     1141 ggggcgagcc tgatcgagat cgaccccgag ccaaccgcgt tgagcgaact cgcgcgtgtt
     1201 gccctccgcg ggccggcggg cgaaatgctc ccgctcctgc tgcgcgcggt gcgcgagcga
     1261 atcgagcggc gatgagtcct cgcctcaacc tcgctctcct cgtgggcgcc gaggacggcc
     1321 tctggtcgat gctgcctggc gatgaagcca aactcgtcat cgagacgcgg agcgtgcgat
     1381 cgctggatgt ccgcggcgat ctcgcaatcg ccggctccgc ggagggcgtg tggctgcatc
     1441 gcaagggcgc ctggaagcag gtgctcgaag gcgacgtgcg ctgcgtgcgc atcggacccg
     1501 aggggacgct cgtcgcgggt gtcgctacct cgactgtgct ggcatcggac gacgagggcg
     1561 ccagctggca cgagtgggag acgctgcaga acctcgtgcg ctaccacggg cagcggatgg
     1621 ggacgcgggc ggcgggagcg ccggtttccg ggatcgcctt cgcgtcgggg atcatcgtgg
     1681 ccttctcggg catcggcgcg ttcctcactc tcgaccgcgg gcgcacctgg gcgttgcact
     1741 cagaaggcct cgaccgccgc gtacacgggc tgtgggacca ccccgaacgg gccgagcggc
     1801 tgtacgcggc cgcgccgagc gggttttacc gcaccgagga tggcggctac tcatgggtgc
     1861 agtcgctgca tggcctggac cgctcatggg cctgcgacat ggccgtgctg cccggggtac
     1921 ccgacacgct cgtgctcagc gccgcgcgcg ccggagacgg cgagggttcg gcgctcttcc
     1981 actcagccga cggggcgatc tcctggacgc ggctgctgct tggcgagaac gacgagtggt
     2041 cacggcctcc gctggtcacc agcctcacca gcccggtcga cgccctgttc gtcctcgcgg
     2101 gccacgaggt gtggggatcc cacgaccgcg gggagcgctg gatcccgatg gcggacggcc
     2161 tgcccctggg gaacacgttc ctcgccgcgg tgggggagtg gtagcaaggc gcacaacctc
     2221 gcccggtgct acgattactg ctcagttttc atcgtcctgg cccgcccgtt ttgtgcaccc
     2281 tccgcctgga cgaacgggga tgggatagct gatgtcgatg gcgaatcgaa cgcaggacca
     2341 gatggtcgag gcgaatggcc tccgccttca tgtccacgaa tggggcgata gccgcaccaa
     2401 gcacgcactg gtgctcctgc acgactacgc cgagacgacc gccgcgtggg aagaagtcgc
     2461 cgtcgatctc tcccgtgaat accgggtgat cgcgatcgac cagcgggggc acgggcgatc
     2521 tgaccgcgcc ggagaccgag actacagtcg agcctcgcag gtcgaggaca tcgccgcggt
     2581 gat
//
LOCUS       DAORPG010000255         2573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4429_length_2573_cov_3.623908, whole genome shotgun sequence.
ACCESSION   DAORPG010000255 DAORPG010000000
VERSION     DAORPG010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4429_length_2573_cov_3.623908"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..152
                     /locus_tag="PLX85_06315"
     CDS             6..152
                     /locus_tag="PLX85_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRC62822.1"
                     /translation="MKAAAAVAGGGGGGRPQLAQAGGKDASKLDEALYAARLAAREKL
                     ASST"
     gene            complement(169..537)
                     /locus_tag="PLX85_06320"
     CDS             complement(169..537)
                     /locus_tag="PLX85_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62823.1"
                     /translation="MPSPVDELLEHDRRGDVVPRPAPTPGIAGGHGGEPLVLVFYGEP
                     DYTPNALSEPSGGDGRGPLVASERKREPDHDPADLIAGGLRTDGGDGSRGGYDLEGDG
                     EALVLVADGEADADSARVDA"
     gene            539..1618
                     /gene="mltG"
                     /locus_tag="PLX85_06325"
     CDS             539..1618
                     /gene="mltG"
                     /locus_tag="PLX85_06325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRC62824.1"
                     /translation="MPGALRNPLVLLVAFLVYLLVLAGIAFTVIQGSPDAVAEELTTA
                     SPDVSFGAGQPVTFVVKAGISASQIAADLEDARVISDRRRFEALAKLTGESADLKAGC
                     YVFSERTPAAEVIVRLREGITSLSSLAVPEGRRVEEVGAALERAGITTADAWRAALQS
                     APRDVLPEAPPGDSLNGYLFPATYPVECRADAPKMVQAMLEAFTAEVTPALVEEAKQK
                     GLSLHQVVTLASIVEREAAIRSEQPIIASVFLNRLDEGMPLQADPTVQYAIATANPPQ
                     GGASWWKTGLTEADLGFESPYNTYLHKGLPPGPIANPGIDAILAVIRPAKTDYLYFVA
                     KGDGSHAFASTLAEHNANVQRYQLP"
     gene            1615..2466
                     /gene="aroE"
                     /locus_tag="PLX85_06330"
     CDS             1615..2466
                     /gene="aroE"
                     /locus_tag="PLX85_06330"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRC62825.1"
                     /translation="MTQRIALLGHPVAHSISPRFQQAALDALAIDARYEAWDVPPADL
                     PIALERLRSAELLGANVTVPHKEAVFRLVDRLDPLAERVGAVNTIVRRDGQLHATNTD
                     VAGILAALAGIPLHGASVLLLGAGGAARAVVVAMHQAGVSRLTIANRTLDRARALAVL
                     APGIEVIPCSLEATDATLREAMAGASLVMQSTTIGMLHGPDEAGSPVPAYWFQPGQTA
                     FDLVYNPERTPFLRAAEAAGARPLGGLAMLVHQGAEAFRLWTGVEPPLDVMFDAARTA
                     MKEMAPR"
BASE COUNT          366 a         1002 c          815 g          390 t
ORIGIN      
        1 agctcgtgaa ggccgcggcc gccgttgccg gcggcggggg tggaggccgc ccgcagctcg
       61 cccaggccgg cggcaaggac gcgagcaagc tcgacgaagc cctctacgcc gctcgcctcg
      121 ccgctcgcga gaagctcgca tcctcgacat aacacgtcga tgcgctggct aggcgtcgac
      181 ccgggcgctg tccgcgtcgg cctcgccgtc tgcgacgagg acgagcgcct cgccgtcccc
      241 ctcgaggtcg tacccgcctc ggctgccgtc cccgccgtcc gtacgcaggc cgcccgcaat
      301 gaggtcggcg gggtcgtggt cgggctcccg ctttcgctcg ctggccacga ggggtcctcg
      361 gccgtcgccg cccgacggct cggagagcgc attcggcgtg tagtcgggct ccccgtagaa
      421 tacgaggacg agcggttcac ctccgtggcc gccggcgatc ccgggcgtgg gggcaggcct
      481 cgggacgaca tcgccgcggc gatcatgctc cagcagttca tcgaccggag aaggcacgat
      541 gccgggcgcg ctccgtaacc cgctcgtcct cctcgtggcg ttcctcgttt acctgctcgt
      601 cctcgcagga atcgccttca cggtcatcca gggttccccc gacgcggtcg ccgaggagct
      661 cacaaccgcc tcgcccgacg tgagcttcgg cgccgggcag cccgtgacct ttgtcgttaa
      721 ggcgggcatt tcagccagcc agatcgctgc agacctcgag gatgcgcggg tcatctccga
      781 ccggcgccgc ttcgaagccc tcgccaagct cacgggcgaa agcgccgacc tcaaagcagg
      841 ctgctacgtc ttctccgaac gcacgcctgc cgccgaggtc atcgtccgcc tccgcgaagg
      901 catcacatcg ctcagctcgc tcgcggtccc ggagggacgc cgcgtcgaag aggtgggtgc
      961 cgccctcgag cgtgccggta tcaccaccgc cgacgcctgg cgcgccgcgc tgcagagcgc
     1021 tccgcgcgac gtcctccccg aggccccgcc cggcgactcg ctgaacggat acctcttccc
     1081 cgccacgtac cccgtggagt gccgcgccga tgcgccgaag atggtgcagg cgatgctcga
     1141 ggcgttcacc gcagaggtca ctcccgcgct ggtcgaggag gcgaaacaga agggcctctc
     1201 gctgcaccag gtcgtcacgc tcgcctcgat cgtcgagcgc gaggctgcga tccgctcgga
     1261 gcagccgatc atcgcctcgg tcttcctgaa ccgcctcgac gagggcatgc cgctccaggc
     1321 cgacccgacg gtgcaatacg ccatcgcgac cgcgaacccg ccgcagggcg gcgcctcctg
     1381 gtggaagacc gggctcaccg aggccgacct cgggttcgag tcgccttaca acacctacct
     1441 ccacaaaggc ctgccgcccg gaccgatcgc caaccccggt atcgacgcca tcctcgccgt
     1501 gatccgcccg gcgaagaccg actacctcta cttcgtggcc aagggcgacg gttcgcacgc
     1561 gttcgcctcc acgctcgcgg aacacaacgc caacgtgcag cggtaccagt tgccgtgacg
     1621 cagcgcatcg ccctcctcgg ccacccggtc gcccactcga tctctccgcg cttccagcag
     1681 gccgccctcg atgccctcgc tatcgacgcc cggtacgagg cgtgggacgt ccctcccgcc
     1741 gacctcccca tcgcgctcga gcgcttgcgc tccgccgagc tgctgggcgc caacgtcacc
     1801 gtcccgcaca aggaagccgt gttccgcctc gtcgaccgcc tcgacccgct cgccgaacgc
     1861 gtcggcgcgg tgaacaccat cgtccgccgc gacggccagc tgcacgccac gaacacggat
     1921 gtcgccggca tcctcgcggc cctcgcgggc atcccgctgc acggcgcgag cgtcttgctc
     1981 ctcggcgccg gaggcgccgc gcgcgcggtc gttgtcgcta tgcatcaagc gggcgtcagc
     2041 cggctcacca tcgcgaaccg cacactggac cgcgcgcgcg ccctcgcggt gctcgccccc
     2101 ggtatcgagg tcatcccctg ctccctggag gccacggacg ccacgcttcg cgaagcgatg
     2161 gccggggcga gcctcgtcat gcagtccacc acgatcggta tgctccacgg cccggacgag
     2221 gcagggtcac ccgtccccgc ttactggttt caaccggggc agaccgcctt cgacctcgtc
     2281 tataaccccg agcgtacccc gttcttgcgt gccgctgagg ccgccggcgc gcgcccactc
     2341 ggcggactgg ccatgctcgt acaccagggt gccgaggcgt tccgcctctg gacgggggtc
     2401 gagccgccgc tcgacgtcat gttcgacgcc gcgcggaccg cgatgaaaga gatggcgccg
     2461 aggtgagctt catcgcggtc ggcatcatcg tcttcctgct cctcggcctg gtgatcggct
     2521 tcacgatcgt ccagtccgcg ttctcccacc ggcactggcg gaaggtcatc gct
//
LOCUS       DAORPG010000256         2572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4431_length_2572_cov_3.481128, whole genome shotgun sequence.
ACCESSION   DAORPG010000256 DAORPG010000000
VERSION     DAORPG010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4431_length_2572_cov_3.481128"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2571)
                     /locus_tag="PLX85_06335"
     CDS             complement(<1..2571)
                     /locus_tag="PLX85_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRC62826.1"
                     /translation="MLTAERARQTLRHVRTVIVDEIHALARDKRGSHLALSLARLDHV
                     TMTANGTRPNRVGLSATQRPIEEIAAFLVGARVRPDGSPDCEVIDLGHQRDLALSIEV
                     PPADLEAVASAEQWADTYDRLAGIIREHRTTLVFVNTRRLAERVAHHLAQRLGEEHVA
                     SHHGSLSKEHRLKLEDKLKAGELKALVATASLELGIDIGSIDLVCQVGSPRSIATFLQ
                     RVGRSGHALGARPEGRLFPTTRDELVECTALVRAVRAGRLDRIVQPVAPLDVLAQQIV
                     AECACEDWAEDALFDLMRSAHPYRDLRREDYDEIVEMLASGVGEGAGRTTPLLHRDRI
                     NGVLRARRGARMVAIQNGGTIPELGDYRVIAEPDETFVGTVNEDFAIESMKGDIFLLG
                     STSWRIMRVEQSQVRVEDAHGAPPTIPFWLGEAPGRTIELSEEVGRLRSDVETGLRDT
                     ELLRQRLEEDCGLDEHGARQLVDYVRATRDGLGLVPTDRDIVFERFFDESGGMQLVVH
                     APFGARINRAYGLALRKRFCVTFDFELQAAASDDAILLSLGPQHSFPLEDAFSFVSPR
                     NAKEAVEQSILYTPLFPTRWRWDATRALAIPRQRGGKRVPPFIQRLRADDLLAAVFPA
                     QVGCQENVTGPLEIPDHPLIRQTVEDCLREAMDIDGLLDVLKRIEEGSIRLHARDTTE
                     PSPMAHEIVNGRLYTYLDDAPLEERRTRAVTLRRALPTEARELGALNLEAIQQVRDQA
                     WPAPRDPEEVHDALLGFIALRLTDVPDWLPALEELAAAGRAAFFERVVPDSGPLPADG
                     EGGGGGASPHAVWFPAEHIGTIRLLYPGADLPFLDIPDAARIPIAEREDARMLLLRGH
                     C"
BASE COUNT          382 a          764 c         1038 g          388 t
ORIGIN      
        1 gcagtggccc cggaggagca gcatccgtgc gtcctcgcgc tcggcgatcg ggatgcgggc
       61 ggcgtcgggg atgtcgagga atgggagatc ggcgccggga tagagcaggc ggatggtccc
      121 aatgtgctcg gcagggaacc agaccgcgtg cggggaggca cccccacccc cgccctctcc
      181 atcagcgggg agggggccgg aatccggaac cacacgctcg aagaaggcgg cgcggccggc
      241 ggcggccagt tcctccaggg cggggagcca gtcggggacg tcggtcaggc gaagagcgat
      301 gaagccgagg agggcgtcgt ggacttcctc ggggtcgcgg ggcgcgggcc aggcctggtc
      361 gcgcacctgt tgaatggctt cgaggttgag ggcgccgagt tcgcgcgcct cggtggggag
      421 agcgcggcga agagtgactg cgcgtgtccg gcgctcttca aggggggcgt cgtcaaggta
      481 ggtatacagg cggccgttca cgatctcgtg ggccatgggc gagggctcgg tggtgtcacg
      541 ggcgtggagg cggatcgagc cctcctcgat ccgtttgagc acgtcgagca ggccgtcgat
      601 gtccatcgcc tcgcgcaggc agtcctcgac ggtctggcgg atgagcgggt ggtcggggat
      661 ctcgaggggg ccggtcacgt tctcctggca cccgacctgc gccgggaaga cggcggcgag
      721 caggtcgtcg gcgcggagcc gctgaatgaa cggcgggacg cgcttgccgc ctcgctggcg
      781 agggatggcg agggcgcgcg tggcatccca gcgccagcgc gtggggaaga gcggggtgta
      841 gaggatcgac tgctcgacgg cctccttcgc gttcctcggg ctcacgaatg agaaggcgtc
      901 ttccaggggg aaggagtgct gggggccgag ggagagcagg atggcgtcat cgctggcggc
      961 cgcctgcagc tcgaagtcga aggtgacaca gaagcgcttg cggagcgcca ggccataggc
     1021 gcggttgatg cgcgcgccga acggggcgtg cacgacgagc tgcataccac ccgactcatc
     1081 gaagaagcgt tcgaagacga tatcgcggtc ggtcgggacg aggccgaggc cgtcgcgggt
     1141 ggcacggacg tagtcgacca gttgacgcgc accgtgttcg tcgaggccgc agtcttcctc
     1201 caggcgctgg cggagcagct cggtgtcgcg aaggccggtc tcgacgtcgg accggaggcg
     1261 ccctacttcc tcggagagct cgatggtgcg gccgggggcc tctcccagcc agaacgggat
     1321 cgtcggcgga gcgccgtggg cgtcctcgac gcggacctgg ctctgctcaa cgcgcatgat
     1381 gcgccaagag gtgctgccaa gcaggaagat gtcgcccttc atgctctcga tggcaaagtc
     1441 ctcgttcacg gtgccaacga aggtctcatc gggctcggcg atgactcggt agtcgccgag
     1501 ctccgggatg gtgccgccgt tctggatggc gaccatgcgc gcacctcgtc gggctcgcag
     1561 gacgccattg atgcggtcgc ggtggagaag gggcgtggtg cgaccggcgc cttcgccgac
     1621 gccggaggcg agcatctcga caatctcgtc gtagtcctcg cggcggagat cgcggtaggg
     1681 gtgggcggag cgcatgaggt cgaagagcgc gtcctcggcc cagtcctcgc acgcgcattc
     1741 ggcgacaatc tgctgcgcca gcacatcgag gggcgcgacg ggctgcacga tgcggtcgag
     1801 gcggccggcg cgcacggcgc gtacgagcgc ggtgcactcg acgagctcgt cgcgggtcgt
     1861 tgggaagagg cggccctccg ggcgggcgcc gagggcgtgg cccgagcggc cgacgcgctg
     1921 gaggaaggtc gcgatgctcc tcggcgagcc gacctgacag acgaggtcga tcgagccgat
     1981 gtcgatgccg agctcgaggg atgcggtggc gacgagagct ttgagctcgc cggcctttag
     2041 tttgtcctca agcttgagcc ggtgttcctt cgagaggctg ccgtggtggc tcgcgacatg
     2101 ctcctcgccg aggcgctgcg cgaggtgatg cgcgacgcgc tcggcgagcc ggcgggtgtt
     2161 cacgaagacc agcgtggtgc ggtgctcgcg gatgattccg gcgaggcggt cgtaggtatc
     2221 ggcccactgc tcggcggaag cgactgcctc gaggtcggcg gggggcacct cgatggagag
     2281 ggcgaggtcg cgctggtggc cgaggtcgat gacctcgcag tcgggtgagc cgtccggccg
     2341 cacgcgcgcg ccgacgagga aggcggcgat ctcctcgatg ggacgctggg tggcggagag
     2401 gccgacgcgg tttggcctcg tcccgttcgc agtcatcgtg acgtggtcga ggcgcgcgag
     2461 cgagagcgcg aggtggctgc cgcgcttgtc gcgggcgagc gcgtggatct cgtcgacgat
     2521 gacggtgcgg acatggcgca aggtctgtcg cgcgcgctcg gcggtgagca tc
//
LOCUS       DAORPG010000257         2561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4465_length_2561_cov_3.560255, whole genome shotgun sequence.
ACCESSION   DAORPG010000257 DAORPG010000000
VERSION     DAORPG010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4465_length_2561_cov_3.560255"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /gene="gltD"
                     /locus_tag="PLX85_06340"
     CDS             complement(<1..459)
                     /gene="gltD"
                     /locus_tag="PLX85_06340"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630672.1"
                     /note="glutamate synthase is composed of subunits alpha
                     and beta; beta subunit is a flavin adenine
                     dinucleotide-NADPH dependent oxidoreductase; provides
                     electrons to the alpha subunit, which binds L-glutamine
                     and 2-oxoglutarate and forms L-glutamate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HRC62827.1"
                     /translation="MAKPTGFMEFGRETPQRRPVGQRVHDWLEVYEDFPTDALRQQAA
                     RCMDCGIPFCHQGCPLGNIIPDWNDLVYRDKWHEAIERLHATNNFPEFTGRLCPAPCE
                     AACVLGINSDPVTIKQVELTIIEHAYREGWIAPEPPRQRTGKSVAVIGSGP"
     gene            complement(452..>2561)
                     /locus_tag="PLX85_06345"
     CDS             complement(452..>2561)
                     /locus_tag="PLX85_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HRC62828.1"
                     /translation="FNPDTVFRLQHATRTGRAEIYREYTQLVNEQSRNLATLRGLFDL
                     KPVGPPVPLEEVEPAGSLFKRFKTGAMSFGSISAEAHETLAIAMNRIGGKSNTGEGGE
                     DRRRFTPDANGDSRRSAIKQVASGRFGVTSEYLVNADEIQIKMAQGAKPGEGGQLPGH
                     KVYPWIAEVRHSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNANPRARISVKLVAEVG
                     VGTVAAGVAKAKSDVVLISGHDGGTGASPLTSLKHAGIPWELGLAETQQTLVLNKLRD
                     RIVVEVDGQMKTGRDVVIGALLGAEEFGFATAPLVVMGCVMMRVCHLDTCPVGIATQN
                     PRLREKFEGRADHVVNYFRFLAEEVREYMAQLGFRSLDEMVGRTDAIDMRRAIDHWKA
                     QGLDYSAILYRPEVGPDVATHCVTTQDHGLEEALDQQLLPLAAPALERGERVHIEMPI
                     RNVNRTVGTILGSELTRKYANTGLPDDTIDIQFHGSAGQSFGAFVPAGMTLRLEGDSN
                     DYTGKGLSGGKLIVHPPREATFIPEENIVIGNVALYGATSGYAFFRGVAGERFAVRNS
                     GANTVVEGTGDHCCEYMTGGRVVVIGRTGRNFGAGMSGGIAFVWDVDGDFRTRCNLEM
                     IDLERLDEPEDLNLVRGLLERHVEYTGSTVAQRLLDRWPAAAAEFVKVMPKEYRRVLN
                     ERARELEEKARGAAMAVGDG"
BASE COUNT          376 a          823 c          911 g          451 t
ORIGIN      
        1 cgggccggac ccgatgacag cgacgctctt cccggtgcgc tgccggggcg gctcgggggc
       61 gatccagccc tcgcgatagg cgtgctcgat gatcgtgagc tcgacctgct tgatggtgac
      121 ggggtcgctg ttgatcccca agacgcaggc ggcctcgcag ggagcggggc agagccggcc
      181 ggtgaactcc gggaagttat tcgtcgcgtg cagacgctcg atcgcctcgt gccacttgtc
      241 gcggtacacg aggtcgttcc agtccgggat gatgtttccg agcgggcagc cctggtggca
      301 gaacgggata ccgcagtcca tgcagcgcgc ggcctgctgg cgcagggcgt ccgtcgggaa
      361 gtcttcgtag acctcgagcc agtcgtggac gcgttggccc acgggacgac gctgcggcgt
      421 ttcgcgcccg aactccatga agcccgtggg cttagccatc gccgaccgcc atcgccgcgc
      481 ctcgagcctt ctcttcaagt tcccgcgcgc gctcgttgag gacgcggcgg tactccttcg
      541 gcatcacctt cacgaactcg gcagccgccg ccggccagcg gtcgagcaga cgctgcgcga
      601 ccgtgctgcc ggtgtactcg acgtggcgct cgagcaggcc gcggacgagg ttgagatcct
      661 cgggctcgtc cagacgctcg aggtcgatca tctcgaggtt gcaccgcgtc ctgaagtcgc
      721 catcgacgtc ccagacgaag gcgatgccgc cgctcatgcc cgcgccgaag ttgcggccag
      781 tgcggccgat cacgacaacg cggccgccgg tcatgtactc gcagcagtgg tcaccggtgc
      841 cctcgacgac cgtgttcgct ccactgttgc gcaccgcaaa gcgctcaccc gcgacgccgc
      901 ggaagaaggc gtacccgctc gtcgcgccat agagggcgac gttgccgatg acgatgttct
      961 cttccgggat gaaggtggct tcccgcggcg ggtggacgat cagcttgcca cccgagaggc
     1021 ccttgccggt gtagtcgttg ctgtcgccct cgaggcggag cgtcatgccc gcgggcacga
     1081 acgccccgaa actctggccg gccgagccgt ggaactggat gtcgatcgtg tcatccggaa
     1141 ggccggtgtt tgcgtatttc ctggtgagct cgctgccgag gatcgtgccg acggtgcggt
     1201 tcacgttgcg gatcggcatc tcgatgtgga cgcgctcacc tcgctcgagg gcaggtgccg
     1261 ccagcggcag cagctgctgg tcgagcgctt cctccaggcc gtggtcctgg gtcgtgacac
     1321 aatgcgtggc cacgtcgggg ccgacctccg ggcgatagag gatggcggag tagtcgaggc
     1381 cctgtgcctt ccagtggtcg atggcgcggc gcatgtcgat ggcgtcggtc cgaccgacca
     1441 tctcgtcgag cgagcggaag ccaagctgag ccatatactc ccgcacttcc tcggcaagga
     1501 agcggaagta gttgacgacg tggtccgcac ggccctcgaa cttctcgcgc aggcgtgggt
     1561 tctgcgtggc gatgccgacc gggcaggtgt cgaggtggca gacgcgcatc atgacgcagc
     1621 ccatgacgac gaggggagcg gtggcgaagc cgaactcctc ggcgcccagg agcgcaccga
     1681 tgacgacgtc gcgtcctgtc ttcatctggc cgtcgacctc gacgacgatg cggtcgcgga
     1741 gcttattcag gacgagcgtc tgctgggtct cggcgaggcc aagctcccac gggatgccgg
     1801 cgtgcttcaa cgaggtcagc gggctcgcgc cagtgccgcc gtcgtggccg ctgatgagca
     1861 ccacgtctga cttcgccttc gcgacgcccg cggcgaccgt gcccacgccc acctcggcga
     1921 caagcttgac gctgatacgc gctcgtgggt tggcgttctt caggtcgtgg atgagctgcg
     1981 ccaggtcctc gatcgagtag atgtcgtgat gaggaggcgg gctgatgagg ccgacgccgg
     2041 gcgtggaatg tcgcacctcc gcgatccagg ggtagacctt gtggccgggc agctgaccgc
     2101 cttcgccggg cttggcgccc tgggccatct tgatctggat ctcgtcggcg ttcacgaggt
     2161 actcgctcgt cacgccgaag cggccggacg cgacctgttt gatcgcgctg cggcgcgagt
     2221 cgccgttcgc gtccggggtg aagcggcgac ggtcctcgcc accctcaccg gtgttgctct
     2281 tgccgccaat gcggttcatg gcgatggcga gcgtctcgtg cgcttcggcg gagatggagc
     2341 cgaaggacat ggcgcccgtc ttgaagcgct tgaacaggct cccggcgggc tcgacttcct
     2401 cgagggggac gggggggccg accggcttga ggtcgaagag gccgcgaagc gtcgcaagat
     2461 tgcggctctg ctcgttcacg agctgcgtgt actcgcggta gatctccgcg cggcccgtgc
     2521 gcgtggcgtg ctggaggcgg aacacggtgt cggggttgaa g
//
LOCUS       DAORPG010000258         2560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4472_length_2560_cov_2.892216, whole genome shotgun sequence.
ACCESSION   DAORPG010000258 DAORPG010000000
VERSION     DAORPG010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4472_length_2560_cov_2.892216"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2160)
                     /locus_tag="PLX85_06350"
     CDS             complement(<1..2160)
                     /locus_tag="PLX85_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase"
                     /protein_id="HRC62829.1"
                     /translation="MLVVGQIDDDGHVVALLTFDDDDFENAYQELERRYYGGEGAPFA
                     EAGAVMSKLVAAGNRGDNDAVFELFEPGMRVESRSRTIIPSRALDELRSSVDEFKAMV
                     RSSRIWCAAVRWLSPTCAVVRHEREAVGHEGQSYEWSRIYALETHGGRFTGGCEFDIE
                     FEDEAFAYAEERLRASASRLAVTNRASVVAERLAKAIRRDSDLEAALAEFASDCAYED
                     RRRLSGDPIVGVFARRAALERVVQQYGHLTMSPVAVRGDRLVLERCCRSDDSGNESIV
                     LVLGEINCDDHVAAYISFDEDDFEGAYRELERRYYAGEGAASAPGGRKLEDFIAALNR
                     GDLDSAFTVISNPDLRIETRSRAVFPDRSAAELREDLDELLSMTSSVRWWYSNICWLS
                     ANVGVGRQEREGIGLEGQGYAWTDIYAFEFQGDAVAWICRYNVEDETQAFADAEARTL
                     LSPRRLAVANRAGAVRVAGTVALQRGEIDTAAGFFSESVVFEDRRRMSGEPPASGSGV
                     RAALTKLRNDYSHFDGQFLAVRGERLVLARYVCRDEAGNQTSGLSLTEIGSDGLNVYE
                     CRYDEDDFGSAYAELERRYYAGEGAAFAVNGMASANYLAAVNRGDFKSAFEEFTSPDL
                     RIESRAKRSGFAVGSASEYQSSFEELKRLVGSFRDWFAAVEWVAPNWMVIRYQRVAVG
                     LNSEDYQWDRICVCEVRNGRLCYICDFDLEDEEAAFAY"
     gene            2125..>2560
                     /locus_tag="PLX85_06355"
     CDS             2125..>2560
                     /locus_tag="PLX85_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62830.1"
                     /translation="MSVVVDLADDEHRRLVTRVIGAAAPGQRKVLPADGNGGQIELIE
                     LLQRTHNRLPPSSFPDDGVATHPTEVSVLGVHGKHLDSPLDVAGSDGVDHAGTDFAGP
                     IGYRKPARPHPHPLLGVCERMLLVLSVELAHRLGQSVPDLHHI"
BASE COUNT          475 a          898 c          735 g          452 t
ORIGIN      
        1 gtaggcgaag gccgcctctt catcctcgag gtcaaagtca cagatgtagc agagacgccc
       61 gttccggact tcgcagacgc agatccgatc ccactggtag tcctcgctgt tgaggccgac
      121 tgccacccgc tggtagcgga tcaccatcca gttcggcgcg acccactcca ccgctgcgaa
      181 ccagtcgcgg aacgagccga ccagtcgttt cagttcctca aaactggatt gatactccga
      241 ggcgctccca accgcgaagc ccgatcgttt tgcgcggctc tcgatccgga gatcggggct
      301 ggtgaattcc tcaaacgcgg acttgaaatc gccgcgattc acggcggcca gatagttcgc
      361 actcgccatg ccgttgaccg cgaacgccgc accctccccg gcgtagtacc ggcgttccag
      421 ctcggcgtac gcggagccga agtcgtcctc gtcgtagcgg cactcgtaga cgttcagccc
      481 gtctgagccg atttcggtca gactcaaccc tgacgtctgg ttgccggctt cgtctctgca
      541 gacatatcgc gccaacacca ggcgttcccc acgcacagcg aggaactgcc cgtcgaagtg
      601 gctgtagtcg ttgcgaagtt tcgtcaaggc ggctcgcacg cccgatccgc ttgccggcgg
      661 ctcgccgctc atccgccttc ggtcttcgaa aacgacgctt tccgaaaaga aacccgcagc
      721 cgtgtcgatc tcgccccgct gcaacgcgac ggtcccagcg acacgaaccg cgcccgcgcg
      781 gttggcgaca gctaaacgcc tggggctgag cagtgtccgc gcttcggcgt ccgcgaatgc
      841 ctgcgtttcg tcctcgacgt tgtaccggca gatccaggcg accgcgtcac cctggaactc
      901 aaaggcgtag atgtcggtcc aggcatagcc ctggccctcc aggccgatcc cttcgcgctc
      961 ctggcggccc acgcctacgt tcgcagacag ccagcagata ttcgagtacc accaccgcac
     1021 tgaggacgtc atcgacagca gttcatcgag atcctcgcga agctcggcgg ccgagcgatc
     1081 cgggaacacc gcgcgggaac gtgtctcgat gcgaagatcc ggattgctga tgaccgtgaa
     1141 agccgagtcg aggtctcccc ggttgagggc cgcgatgaaa tcctccagct ttctcccgcc
     1201 gggagccgaa gctgcgccct cgccggcgta gtagcgacgc tcaagctctc ggtaggcacc
     1261 ctcgaaatcg tcttcatcga agctgatgta ggcggccacg tgatcgtcgc aattgatctc
     1321 gccgaggacg agcacgatgg attcgtttcc ggaatcgtca gagcggcaac acctctcgag
     1381 gactaggcgg tccccgcgca ccgctaccgg gctcatcgtc aggtggccgt actgctgtac
     1441 tacccgctca agtgccgcgc gccgcgcgaa gacaccaacg atcggatcgc cgctgaggcg
     1501 acgccgatcc tcgtacgcgc aatcgctcgc aaactccgcg agagcagctt ccagatcaga
     1561 gtcgcgccgg atcgccttcg ccagacgttc ggcgaccacg ctagcccggt tcgtcaccgc
     1621 cagtcggctg gccgaggcac gcaggcgctc ctcggcgtac gcgaacgcct cgtcttcgaa
     1681 ctcgatatcg aattcgcaac cgcccgtgaa ccgtccgccg tgagtctcga gagcgtagat
     1741 ccgcgaccac tcgtagctct gaccctcatg accgacggct tcacgctcat gccggacaac
     1801 cgcacaggtg ggcgacagcc agcgcaccgc cgcgcaccag atccgcgatg agcgaaccat
     1861 ggccttgaac tcatccacac tggaacgcaa ctcgtccaat gcccgactcg ggatgatggt
     1921 gcgggagcgg ctctccaccc gcattcccgg ttcgaacaac tcgaacaccg cgtcgttgtc
     1981 gccgcggttg cccgccgcca ccagcttgga catcacggcg cccgcttctg caaacggcgc
     2041 gccttcaccg ccgtagtagc gacgctccaa ttcctgatag gcgttctcga aatcatcgtc
     2101 gtcgaaggtg aggagtgcga cgacgtgtcc gtcgtcgtcg atctggccga cgacgagcac
     2161 cgtcgactcg ttacccgcgt catcggagcg gctgctccgg gccagcgcaa ggttctcccc
     2221 gcggacggca atggtggtca gatcgaattg attgaactgt tgcagcgaac gcacaatcgc
     2281 ctgccgccga gcagctttcc cgacgatggg gtcgccactc acccgacgga agtctccgta
     2341 ctcggagtcc acgggaaaca tctcgacagc ccgctcgacg tcgccggctc ggatggcgtc
     2401 gaccatgcgg gaaccgattt cgctggcccg attggctatc gcaagccggc tcggcctcac
     2461 ccgcatccgc tcctcggcgt atgcgaacgc atgctcctcg tcctcagcgt cgaactcgca
     2521 caccgattgg gccagtccgt tccggatctc catcacataa
//
LOCUS       DAORPG010000259         2559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4476_length_2559_cov_3.039936, whole genome shotgun sequence.
ACCESSION   DAORPG010000259 DAORPG010000000
VERSION     DAORPG010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4476_length_2559_cov_3.039936"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..875)
                     /gene="argB"
                     /locus_tag="PLX85_06360"
     CDS             complement(102..875)
                     /gene="argB"
                     /locus_tag="PLX85_06360"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRC62831.1"
                     /translation="MSETSKRPIVVKIGGSTLGAEDTTLDDVVALHQAGERVAVVHGG
                     GAMITDWLTRLDVPSEFVDGLRSTSAEAMEVVVAVLRGVINTQLVAAIVQRGGRAVGL
                     SGVDGGVVRAERYDPRLGYVGRVTSVDGAFLGALMEAGAIPVIAPVGLEAPEQPLNIN
                     ADTVAGEVARAVGARQLVFLTDVDGLLDGDGALIPALDAERAEALRAAGTLSGGMIPK
                     VEACFRAAEAGAVAHMVNGRTGGTLRRVAGGEAIGTRIA"
     gene            complement(894..2198)
                     /gene="argJ"
                     /locus_tag="PLX85_06365"
     CDS             complement(894..2198)
                     /gene="argJ"
                     /locus_tag="PLX85_06365"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HRC62832.1"
                     /translation="MTEFLRLRGPGLFVLGYTRGSMVSEGRAVAVELEWVEGGGVTTP
                     LGWRAGAAYAGVRTYGEEPRYDVGILVSEQPASVAGIFSRNGVVGAPIKYNRARVVTG
                     RARGIVANSGNANTVTGQQGDRDAARMAELAARQSGIEVEEMLVASTGVIGRIMPMDK
                     IEAGIASIQPSADGGALFTRAIMTTDTVPKHRAARVTSGGRTYTVGGTAKGSGMVHPD
                     MATVFCFLTTDAPCELGWLQQTLREVGDLSINMIDVDMDSSTSDTMLLFANGAAGGEP
                     IDATHPASGPLREAIEALAIALARDLARDGEGARTLIEVTVEGAATREDARKAARTVT
                     SSPLVKTMVTGRDANLGRVLMAVGRSGARVEVERASVWIGPYCAFERGVPTDVDYAVL
                     SKAMDAEEVKIRVELGLGEERATAWGCDLTEDYIRINADYTT"
BASE COUNT          377 a         1080 c          739 g          363 t
ORIGIN      
        1 ggaagcgtcg gccctcgcgg tcgaaccgga aggggtcagg cggaggcccg aggtcgccgc
       61 ggtcctcgta gaactgcatc aggcgccggc cgagccagtc atcaggcgat ccgggtcccg
      121 atcgcctcgc cgcccgccac ccgccgcaac gtcccccccg tgcggccgtt caccatgtgc
      181 gcgaccgccc ctgcctcggc cgcccggaaa cacgcctcca ccttcgggat catcccgccc
      241 gagagcgtcc ctgccgcccg cagggcttcg gcgcgttccg cgtcgagcgc cgggatcagc
      301 gccccgtccc cgtcgaggag cccgtcgacg tccgtgagga acaccagctg ccgcgcgccg
      361 acggcccgcg ccacctcgcc ggccaccgtg tccgcgttga tgttcagcgg ctgctccggc
      421 gcctcgaggc cgaccggagc gatcaccggg atcgcccccg cctccatcag cgcccccagg
      481 aacgccccgt ccaccgaggt gacgcgcccc acgtacccca gccgtgggtc gtaccgctcc
      541 gcccgcacca cgccgccgtc gaccccggac agcccgaccg cccggccgcc gcgctgcacg
      601 atcgccgcta cgagctgcgt gttgatcacc ccgcgcagta ccgcgaccac gacctccatc
      661 gcctccgccg aggtcgaccg cagcccgtcc acgaactccg agggcacgtc gagccgcgtc
      721 agccagtccg tgatcatcgc tccgccgcca tggacaacgg caacgcgctc ccccgcctgg
      781 tgcagcgcca ccacgtcatc gagtgtcgtg tcttccgcgc ccagcgtgga accgccgatc
      841 ttcaccacaa tcggccgctt cgaggtctca ctcatctcgt tcgtctcatt cagttatgtc
      901 gtgtagtccg cgttgattcg gatgtagtcc tcggtcaggt cgcagcccca ggccgtcgcc
      961 cgttcctccc ccagccccag ctccacgcgg atcttcactt cttctgcgtc catcgccttc
     1021 gagagcaccg cgtagtccac gtccgtcggc acgccccgct cgaacgcgca gtacggcccg
     1081 atccacacgc tcgcacgctc cacctcgacc cgcgcccccg accgcccgac ggccatcagc
     1141 acccgcccga ggttcgcgtc gcgcccggtc accatcgtct tcaccagcgg cgacgaagtc
     1201 accgtccgcg ccgctttccg cgcgtcctca cgcgtcgccg cgccctcgac cgtcacctcg
     1261 atcagcgtcc gcgccccttc gccgtcccgc gcgaggtcac gcgccagcgc gatcgccagc
     1321 gcctcgatcg cctcgcgcaa cggccccgag gccggatgcg tcgcgtcgat cggctcgccc
     1381 ccggccgccc cgttcgcgaa cagcagcatc gtgtcgctcg tcgaggaatc catgtcgacg
     1441 tcgatcatat tgatcgacag gtcccccacc tcgcgcagcg tctgctgcag ccacccgagt
     1501 tcacagggtg catccgtcgt caggaagcag aacaccgtgg ccatgtccgg atgcaccatc
     1561 ccggacccct tcgccgtccc tccgaccgtg tacgtgcgcc cgcccgaggt cacccgcgcc
     1621 gcccgatgct tcggcaccgt gtccgtcgtc atgatcgccc gcgtgaacag ggccccgccg
     1681 tctgcgctcg gctgaatcga ggcgatcccg gcctcgatct tgtccatcgg catgatccgc
     1741 ccgatcaccc ccgtcgaggc gaccagcatc tcctctacct cgatgccaga ctgccgcgcc
     1801 gccagctcgg ccatccgcgc cgcgtcgcgg tctccctgct ggccggtgac cgtattcgca
     1861 ttccccgagt tcgcgacgat gccccgcgct cgccccgtca ccacccgtgc ccggttgtac
     1921 ttgatcggcg cgccgaccac gccgttgcgg ctgaagatcc ccgccaccga cgccggctgc
     1981 tccgacacca ggatccccac gtcgtagcgg ggctcctcgc catacgtacg cacccccgcg
     2041 tacgccgccc ccgcccgcca tccgaggggc gtcgttacac cgccgccctc aacccactcc
     2101 agctccacag ccacggctcg cccttccgac accatcgagc cgcgagtata gccgagcacg
     2161 aacaaccccg gccccctgag gcgaaggaac tcggtcacag accttcgagg agcgcagcgc
     2221 aaaagcagcg ccgccgcaaa cctcggtgga aggtgaggta ggaggctcta aggaacggcc
     2281 gcgcctcagc acaacgtcga cccgagccca ccaacaccgc aatggaaggg gcgatccccc
     2341 ggccgggcgg gtgagcgggc tcaaacgcca gccggctccg ccagcctgac gaccgtcgct
     2401 ccgcgtcgtc cctctacctc gccgccacgt ccgttaccgc cccgcgcagg cgtagctcgg
     2461 tcgggttcga gggggttcag gggacgaagg cccctgaggg gtgggcaggc gggcctccca
     2521 cacaacaaca gccgggccgc gcagtggccc gtgcacacc
//
LOCUS       DAORPG010000260         2559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4477_length_2559_cov_3.033946, whole genome shotgun sequence.
ACCESSION   DAORPG010000260 DAORPG010000000
VERSION     DAORPG010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4477_length_2559_cov_3.033946"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1289)
                     /locus_tag="PLX85_06370"
     CDS             complement(3..1289)
                     /locus_tag="PLX85_06370"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC62833.1"
                     /translation="MPRLRLPFYGWWIVGAAIGAQALSAGLLQQSFGSYIVLLERDFG
                     WNRTALSGAFSLARVEDGLLGPIQGWLLDRLGPRAVMRAGMLMFAAGFFLFARIDTIT
                     WFYIAYLVMAVGASFAGFLSITTVIVNWFEEKRQTAMGIALLGTAIGGFSLPLVVWGL
                     ETWGWRTVANVSGIIVLLVGLPLTQVVRQRPEPYGYLPDGRKPEDSGAAAASVVRLSS
                     NIPDFTVRQALRTKAFWYISLAHAASVLVVAVVQVHFTAHLVGSLSYSLGAAATLTTV
                     MTAANFAGRPLGGWLASRIGSRAVIMGCMLLHAGSLLALAYGTAMPVLVGFAIGNGLA
                     WGARVPVIVTMRAEYFGAKSFGSIMGLSSSVVTIASVSAPFLAGLSYDVTGSYTFGFT
                     ALAIAAALGTLFLVFLPRPEVTPTSTPTAPLAAAHS"
     gene            1518..2549
                     /locus_tag="PLX85_06375"
     CDS             1518..2549
                     /locus_tag="PLX85_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC62834.1"
                     /translation="MHRRVLILLTAFALGAALLAACGGDDDKGGANATATAGIGSASS
                     PTATAAAVYPMTVKDMLGKDVTIKAQPQRIVAASPSAIELLYAAGGKAVARTTTAKIP
                     AEVTSLPDIGTAEKPAYERIVQQNPDLVLADASLQAQFAPNFASSLAGVPVLFVGAQK
                     YDDIATSLQLIGKVINNPEKAASAVKAMEDAKTQVKNLVSGKTAPRTLILIGAPNDPF
                     VALTDSFIGNLAGLAGAKNVAAGEPQSGPLPGYTKLSLEKIVAADPEVILTITAGPPG
                     GPSLADGIKSNAAYATLSAVKNGRVRDLDVEVYLQSPSPRAAQGLKDLAALLFPGAGA
                     SATVSRLCY"
BASE COUNT          470 a          719 c         1042 g          328 t
ORIGIN      
        1 gtctatgagt gggcggccgc gagcggcgcg gtgggtgtgg aggtgggggt gacttcgggc
       61 ctggggagga agacgaggaa gagggtgccg agggcggcgg cgatggcgag cgcggtgaag
      121 ccgaaggtgt aggagccggt gacgtcgtag gagagcccgg cgaggaaggg cgcggagacc
      181 gaggcgatgg tcacgacgct ggaggagagg cccatgatcg agccaaagga tttcgcgccg
      241 aagtattcgg cacgcatggt gacgatgacg gggacgcggg cgccccaggc gaggccgttg
      301 ccgatggcga agccgacgag caccggcatc gcggtgccgt aggcgagcgc gagcagggag
      361 ccggcgtgca gcagcatgca gcccatgatc acggctcgtg agccgatgcg gctcgccagc
      421 caaccgccga gggggcgtcc cgcgaagttg gccgcggtca tgacggtggt gagggtggcg
      481 gcggcgccga gggagtagga gagcgagccg acgaggtgcg cggtgaagtg cacctggaca
      541 accgcgacca cgaggaccga cgcggcgtgg gcgagggaga tgtaccagaa ggccttcgta
      601 cggagggcct ggcggacggt gaagtcgggg atgttggagg agagacggac gaccgaggct
      661 gcggcggcgc cggaatcctc gggcttgcgg ccgtcgggta ggtagccgta gggctcgggg
      721 cgctggcgga cgacctgggt gagggggagg ccgacgagca ggacgatgat gccggagaca
      781 ttggcgacgg tgcgccagcc ccaggtctcg aggccccaga cgacgagggg gagtgaaaag
      841 ccgccgatgg cggtgccgag gagcgcgatg cccatcgcgg tctgccgttt ctcctcgaac
      901 cagttgacga tgacggtggt gatcgagagg aagccggcga aggaggcgcc gacggccatg
      961 accaggtagg cgatgtagaa ccaggtaatg gtgtcgatgc gggcgaagag gaagaagccg
     1021 gcggcgaaca tcagcatgcc ggcgcgcatg acggcgcggg ggccgaggcg atcgaggagc
     1081 cagccctgga tcgggccgag gagtccatcc tcgacgcggg cgagggagaa ggcgccggag
     1141 agggcggtgc ggttccagcc gaagtcgcgc tcgagcagga cgatgtagga gccgaaggac
     1201 tgctgcagga ggccggcgga gagggcctgc gccccgatcg cggcgccgac gatccaccaa
     1261 ccgtagaagg gcaggcggag cctcggcacg gacgagcctt tcggtgcggg aagtgcaacg
     1321 ggaggagccc gctggagggc gatgataacg agcggagatg cagggtgcat ccgtcgcagg
     1381 cgaccttggc gagccggtcg tagaacctca aatctcgtcg aggttcggat agctttccgg
     1441 cttttagggg cttccgtcca ttgacgctag atcggggccg ccgaatggtg agtgccgccc
     1501 gagaaaggac ggaaaccgtg caccgacgtg tcttgatctt gctgacggcg tttgccctcg
     1561 gcgcggcgtt gctggccgcg tgcggcggcg acgatgacaa gggcggggcg aacgcgacag
     1621 cgaccgcggg catcgggagc gcctcttcgc cgacggcgac ggcggccgcc gtgtacccga
     1681 tgaccgtgaa ggacatgctg ggcaaggacg tgacgatcaa ggcgcagcca cagcgcatcg
     1741 tggcggcgag cccctcggcg atcgagttgc tgtacgcggc gggcgggaag gccgtcgcgc
     1801 gcacgacgac ggcgaagatc cccgctgaag tgacttcgct gccggacatc ggcacggcgg
     1861 agaagccggc gtacgagcga atcgtgcagc agaacccgga cctcgtgctt gcggacgcct
     1921 cgctgcaggc gcagttcgcg ccgaacttcg cgagcagcct cgcgggggtg ccggtgctgt
     1981 tcgtgggggc gcagaagtac gacgacatcg cgacctcgct gcagctgatc gggaaggtga
     2041 tcaacaaccc cgagaaggcc gcctccgcgg tgaaggcgat ggaggacgcg aagacgcagg
     2101 tcaagaacct ggtgagcggg aagaccgcgc cacggacgtt gatcctgatc ggcgcgccaa
     2161 acgacccatt cgtggcgctg acggactcgt tcatcggcaa cctcgcgggg ctggcggggg
     2221 cgaagaacgt cgcggccggg gagccgcaga gtgggccgct gccggggtac accaagctgt
     2281 cgctggagaa gatcgtggcg gcggacccgg aggtgatcct cacgatcacg gcgggccctc
     2341 caggcggtcc gagcctggcg gacgggatca agagcaacgc cgcgtacgcg accctttcgg
     2401 cggtcaagaa tgggcgggtg cgtgacctcg acgtggaggt gtacctgcag tcgccgagcc
     2461 cgcgggcggc ccaggggttg aaggacctcg cggcgctgct cttcccgggc gcgggcgctt
     2521 cggcgacggt gtctcgactt tgttactgaa aacccaggt
//
LOCUS       DAORPG010000261         2534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4551_length_2534_cov_4.028641, whole genome shotgun sequence.
ACCESSION   DAORPG010000261 DAORPG010000000
VERSION     DAORPG010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4551_length_2534_cov_4.028641"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..485)
                     /gene="greA"
                     /locus_tag="PLX85_06380"
     CDS             complement(12..485)
                     /gene="greA"
                     /locus_tag="PLX85_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRC62835.1"
                     /translation="MSEEPVLLTKAGLERLETELNELRTVRRAEVAAAIREAQELGTA
                     QIDGQYEEAKNQQAFLEGRIREIEKMLELAQVIDEEAAHHSKEVRVGSTVTVKMDDTR
                     KHTYQVVGPAEAAPLEGRISHESPVGRALLGKKRGDKVQVNAPSGVVEMTIVSVS"
     gene            670..1872
                     /locus_tag="PLX85_06385"
     CDS             670..1872
                     /locus_tag="PLX85_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRC62836.1"
                     /translation="MSVSTRTALAARGPGGRIPQPAASRLPRSKESLIRQGTLLPLIL
                     ALAVGPVAGLAGGVIGGRLTDSDSEGDAAEATPAPDTAARVRAAVDRVLPNVVLILAD
                     LPNTQDAQGRTVERQNIGSGVVVSQDGHILTNSHVVSGAVSISVFLSTGEQRPARLLA
                     DDAPFTDLAMLQVDPKGLRQIAFGASASLRPGDAVVAVTGGGGIFGPGNAVTTGVVSA
                     IDRTLPRSGVTFEDLIQTDTVVNSGDSGGALVNLSGEFVGLLTTVVRDTGSGVPAEGI
                     AFAQSSDSLRAIVADIVRTGKHARPRIGIEFPDRQHLEITAELAAERSLPVAEGALVV
                     TVPAGSPAAAAGVQPGDIVVAVNGAAVTGQQPMVNLLKLLPRGARVDLVLLRGGRQVT
                     VSITPSEG"
     gene            1912..>2534
                     /locus_tag="PLX85_06390"
     CDS             1912..>2534
                     /locus_tag="PLX85_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62837.1"
                     /translation="MPQSRFEDEDTAAADSEYDDEWPPAPPARRGDGGSFEADRNLLR
                     VAGLIGILAAVILILVLPPISILDRGNGGTTAAPGEVVTKARKSLPALPEGIEAASAF
                     YDIAASASNQGPATLTVRLSQQTNDGRNLAFYTYADGQWERLAAVEPADGGRAARGEV
                     PFVPSNIAVLRRTSFARTPGLIIAPGQTPDPAAPTGGVISVLAGAPAA"
BASE COUNT          363 a         1009 c          752 g          410 t
ORIGIN      
        1 gggtggagtg actacgagac cgagacgatc gtcatctcga cgacgccgct cggcgcgttc
       61 acctgcactt tgtcaccacg cttcttgccg agcaatgccc gcccgaccgg cgattcgtgg
      121 ctgatccggc cctccagcgg cgcggcctcc gcgggaccca ccacctggta cgtgtgcttt
      181 cgcgtgtcgt ccatcttcac ggtcaccgtc gagcccacgc gcacttcctt cgagtggtgc
      241 gccgcctcct cgtcgatcac ctgcgccagc tcgagcattt tctcgatctc gcggatgcga
      301 ccctcgagga acgcctgctg gttcttcgcc tcttcgtact ggccgtcgat ctgcgcggtc
      361 cccagctcct gcgcctcacg gatcgccgcc gccacttcgg cacgacgtac cgtgcgcagc
      421 tcgttcagct ccgtctcgag gcgctccagc cccgctttgg tcagcagcac cggctcttca
      481 gacatcgcac atccttcgcg gcggtctccc ccaccgcttc tcctgtcgca cgtgattgcg
      541 aacgcgccgc ggagctactt ggagccgcgg cggcaaacac gaaacggact cgcccgcgag
      601 gccagtccgt cggatgcacc cgaacgggcg tacgtatcgt acgtgacgat aactacccct
      661 gacaatatcg tgagcgtatc gacgcggacc gccctcgcgg cgcgcggccc cggtggtaga
      721 atccctcagc cggcggcatc tcggctccca cgttcgaagg agtccctcat ccgccaggga
      781 accctgctcc cgctgatcct cgcgctggcc gtcgggcccg tggccggcct cgctggcggc
      841 gtcatcggcg gccgtctcac tgactccgat tccgagggcg atgccgccga ggcgacaccc
      901 gccccggaca ccgcggcgcg cgtccgcgcg gccgtcgacc gcgtcctgcc caacgtcgtc
      961 ctgatcctcg ccgacctccc gaacacccag gacgcccagg gacgcaccgt cgaacggcag
     1021 aatatcggct caggcgtcgt cgtcagtcag gacggccaca tcctcacgaa ctcgcacgta
     1081 gtcagcggcg ccgtctcgat cagcgtcttt ctctcgaccg gcgagcagcg tccagcccgc
     1141 ctactcgccg acgatgcccc cttcaccgac ctcgcgatgc tccaggtcga ccccaagggg
     1201 ctccggcaga tcgccttcgg cgcgtcggcc tcgctcaggc caggtgacgc cgtcgtcgcc
     1261 gttacgggag gcggcggcat cttcggcccc ggcaacgccg tcaccacggg cgtcgtctcc
     1321 gccatcgacc gcaccctccc gcgcagcggc gtcacgttcg aggacctcat ccagaccgat
     1381 acggtcgtca acagcggtga ttccggaggc gcgctggtca atctcagcgg cgagtttgtc
     1441 ggtctcctca cgaccgtggt ccgggacacc ggcagcggcg ttcccgccga aggtatcgcc
     1501 ttcgcgcaat cgtccgactc cctccgcgcc atcgtggccg acatcgtccg caccggtaag
     1561 catgcccgcc cgcgcatcgg catcgaattc cctgatcgcc agcacctcga aatcacggcg
     1621 gaactcgccg ccgaacgcag cctccccgtc gccgaaggcg cgctcgtcgt caccgtgccc
     1681 gctggctctc cagccgccgc cgcgggcgtg caacccggcg atatcgtcgt cgccgtgaac
     1741 ggcgcggccg tcacggggca gcagccaatg gtgaatttgc ttaaactcct gccacgaggt
     1801 gcgcgtgttg acctcgtgct cctgcgcggc ggccgccagg tcaccgtgtc catcacacct
     1861 tcggagggct aactgacgat gcgagaccca cgccggccac gcctcccacg gatgccgcag
     1921 tcccgcttcg aggacgagga caccgccgcc gcggactctg agtacgacga cgagtggccc
     1981 ccggcgccgc ccgcccgccg aggcgacggc ggcagcttcg aggccgatcg caacctgctc
     2041 cgtgtggccg gcctcatcgg catcctcgcg gccgtcatcc tgatcctcgt cttgccgccc
     2101 atctccatcc tcgaccgtgg caacggcggt accacggccg cgcctggcga ggtcgtcacc
     2161 aaggcccgca agtcgctccc cgccctcccc gagggcatcg aggcggccag cgccttctac
     2221 gacatcgcgg cgtccgcctc caaccagggg ccggccaccc tcactgtccg tctgtcgcag
     2281 cagacgaatg acggccgcaa cctcgccttc tacacctacg ccgacggcca gtgggaacgc
     2341 ctcgccgccg tcgagccggc cgatggcggt cgagccgcgc gtggcgaggt ccccttcgtc
     2401 ccctcgaaca tcgccgtcct ccgccgcacg agtttcgccc gcacgcccgg cctcatcatc
     2461 gctcccggcc agacgcccga cccggcggct ccaacgggtg gtgtgatcag cgttcttgcc
     2521 ggcgcgcctg ccgc
//
LOCUS       DAORPG010000262         2534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4553_length_2534_cov_3.682130, whole genome shotgun sequence.
ACCESSION   DAORPG010000262 DAORPG010000000
VERSION     DAORPG010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4553_length_2534_cov_3.682130"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PLX85_06395"
     CDS             <1..365
                     /locus_tag="PLX85_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HRC62838.1"
                     /translation="HRVAGETYLEGLRSALEQPEFASAERMLDAVRHLQAYELLRALP
                     TPEELRPGATRVMIGSENSDDWMHEWSVVTSSFGDYNGSLGTIAVLGPTRMRYGYTIP
                     RVRYVASLVTALLQGMSR"
     gene            393..965
                     /locus_tag="PLX85_06400"
     CDS             393..965
                     /locus_tag="PLX85_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRC62839.1"
                     /translation="MTNEPTDSMPPDAIGATAGAPDDDVDALRAELEEARSKYMRAAA
                     DYQNLRRRSEEERKEYARYTLAALVINYLPVLDDLDRALESVHEEIAEHQWVEGVRMV
                     ERKFRAVLEASGVREIGADGSAFDPAVHEAISYAPGPAGQVVAVVQPGYTLDGKVVRP
                     AQVVVGSGAGGTGGFSDGSNQNEATTESAE"
     gene            1005..>2534
                     /gene="dnaK"
                     /locus_tag="PLX85_06405"
     CDS             1005..>2534
                     /gene="dnaK"
                     /locus_tag="PLX85_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRC62840.1"
                     /translation="MAKVIGIDLGTTNSCVAVMEGGEPQVIPNAEGGRTTPSIVAVSK
                     TGERLVGTVAKRQAVTNPDSTIFSVKRLMGRKFEDAEVQHLKAVAPFPIERAANGDAV
                     VVMGGRQYSPPEISAMILQKLKADAEAYLGETVTEAVITVPAYFNDAQRQATRDAGRI
                     AGLEVLRIINEPTAASLAYGLDKKADETIAVYDLGGGTFDISVLELGEGTFQVKATNG
                     DTFLGGDDFDNVVINYLIAEFKREQGIDLAQDRMALQRLKEAAERAKIELSTTQQTEV
                     NLPFITADAAGPKHMAITIPRSKLEQLVGDLVDRSLEPCQKALQDASLSKASIDEVVL
                     VGGMTRMPLVQSKVQEFFGKEPHKGVNPDEVVAIGAAIQAGVLRGEVKDVLLLDVTPL
                     TLGIETLGGVMTPLIERNTTIPTSAKQVFSTAADNQPSVEIHVLQGERSMAGDNKSLA
                     RFILDGILPAPRGVPQIEVEFNIDANGIVTVAARDKATAKEQHITIQPASGLSKDEVE
                     QL"
BASE COUNT          481 a          799 c          901 g          353 t
ORIGIN      
        1 agcatcgcgt cgccggtgag acatacctcg aggggctgcg ctcggcgctc gagcagccgg
       61 agttcgcatc ggcggagcgg atgttggacg cggtgcgcca cctgcaggca tacgagctgc
      121 tgcgcgcgct gcccacgccc gaggagcttc ggcccggcgc gactcgagtc atgatcggca
      181 gcgagaacag tgacgactgg atgcacgagt ggagcgtggt gacgtcctcg ttcggcgact
      241 ataacgggtc gcttgggacg atcgccgtgt tggggcccac ccgcatgcgg tacggataca
      301 ccattccgcg cgtccgatac gtagcttcgc tggtgacagc actgctgcaa gggatgagcc
      361 ggtaacgccg gcccccgctg acgaggaaac gcatgacgaa tgaacccacg gacagcatgc
      421 ccccggacgc gatcggcgcg acggccggag cgcccgacga cgacgtcgac gcgctgaggg
      481 cggagctgga ggaagcacgc agcaagtaca tgcgggcagc cgcggactat cagaacctgc
      541 gccgccgatc agaggaggag cgcaaggaat acgcgcgcta cacgctcgcg gcgctcgtga
      601 tcaattacct gccggtgctc gacgacctcg accgggcact cgagtccgtg cacgaggaga
      661 tcgccgagca ccaatgggtc gagggcgtgc gtatggtgga gcggaagttc cgcgcggtcc
      721 tcgaggcgag cggcgttcgc gagatcggag cggacggttc cgcattcgac ccggcggtac
      781 acgaagcgat ctcgtatgcg ccgggaccgg cgggtcaggt cgtcgcggtc gtacaaccgg
      841 gctacacgct ggacggcaaa gtggtgcggc ccgcccaggt cgtcgtgggt agcggcgccg
      901 gcgggaccgg cggcttctcg gacggctcga accagaacga agcaactacc gagagcgcgg
      961 agtagaccgc gcgccaaccc gtttgatcgg gaggatgggc agagatggcg aaggtcattg
     1021 gtatcgacct cgggacgacg aactcctgcg tggcggtgat ggaaggcggc gagccgcagg
     1081 tcatcccgaa tgcggaaggc gggcgcacga cgccctcgat cgtcgcggtg agcaagacgg
     1141 gggagcgcct cgtcggcacc gtcgcgaagc gccaggcggt cacgaacccg gacagcacga
     1201 tcttctccgt gaagcggctc atggggcgca agttcgagga cgccgaggtg cagcacctca
     1261 aggccgtcgc tcccttcccc atcgagcggg ccgccaacgg cgacgcggtg gtggtcatgg
     1321 gtggacgcca gtactcgccg cccgagatca gcgccatgat cctgcagaaa ctgaaggcgg
     1381 acgctgaggc gtacctcggt gagacggtga ccgaggccgt gatcaccgtg ccggcgtact
     1441 tcaacgacgc ccagcggcag gcgacccgtg acgcgggccg catcgctggg ctcgaggtgc
     1501 tgcgcatcat caacgagccg acggcggcct cgctcgcgta cggtctcgat aaaaaggctg
     1561 acgagacgat cgcggtgtac gacctcggtg gcggcacctt cgacatctcg gtgctcgagc
     1621 tgggtgaggg gacgttccag gtcaaggcca cgaacggcga cacgttcctc ggcggcgacg
     1681 acttcgacaa cgtcgtaatc aactacctga tcgcggagtt caagcgcgag cagggcatcg
     1741 acctggcgca ggaccgcatg gcgctccagc gcctgaagga ggccgccgaa cgcgcgaaga
     1801 tcgagctgtc gacgacgcag cagaccgagg tcaacctgcc gttcatcacc gccgacgccg
     1861 ccggcccgaa gcacatggcg atcaccattc cgcgctcgaa gctcgagcag ctcgtgggcg
     1921 acctcgtgga ccgctcgctg gagccgtgtc agaaagccct gcaggacgcc agcctgagca
     1981 aggcgagcat cgatgaagtc gtgctcgtcg gcggcatgac gcgtatgccg ctcgtgcagt
     2041 cgaaggtgca ggagttcttc ggcaaggagc cgcacaaggg cgtgaacccg gacgaggtcg
     2101 tcgccatcgg cgcggcgatc caggcgggcg tgctgcgtgg cgaggtgaag gacgtcctgc
     2161 tcctcgatgt cacgccgctg accctgggca tcgagacgct gggcggcgtg atgaccccgc
     2221 tcatcgagcg caacacgacg atcccgacga gcgcgaagca ggtgttctcg acggcggcgg
     2281 acaaccagcc ttccgtggag atccacgtcc tgcagggcga gcgctcgatg gcgggcgaca
     2341 acaagtcgct ggcgcgcttc atcctcgatg gcatcctgcc cgcgccgcga ggcgtgccgc
     2401 agatcgaggt cgagttcaac atcgatgcga atggcatcgt gacggtcgcg gcgcgcgaca
     2461 aggcgacggc caaggaacaa cacatcacga ttcagcccgc ctcgggcctg tcgaaggacg
     2521 aggtcgaaca gctg
//
LOCUS       DAORPG010000263         2532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4568_length_2532_cov_3.585789, whole genome shotgun sequence.
ACCESSION   DAORPG010000263 DAORPG010000000
VERSION     DAORPG010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4568_length_2532_cov_3.585789"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLX85_06410"
     CDS             complement(<1..251)
                     /locus_tag="PLX85_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRC62841.1"
                     /translation="MPDSRIRNFSIIAHIDHGKSTLADRMLEFTGAVDARDMQEQLLD
                     SMDLEREKGITIKAQAVRMPYLAKDGQRYQLNLIDTPGHV"
     gene            complement(348..1373)
                     /locus_tag="PLX85_06415"
     CDS             complement(348..1373)
                     /locus_tag="PLX85_06415"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HRC62842.1"
                     /translation="MTTTESTTSQATDGANTQRRGLIDVNNLKMYFPVTAGVLVQRKV
                     AEVKAVDDVSFFVRKGETLGLVGESGCGKSTTGRAVLQLYKPSGGSVNFDGTELTTLK
                     PKALRSFRRRMQMIFQDPYSSLNPRMNVQSIIAEPMAIHGLHKGGERRDRISYIMNAV
                     GLNPYFAKRFPHEFSGGQRQRIGIARALAVEPDFIVADEPVSALDVSIQAQIVNLMED
                     LQTEFNLTYLFIAHDLAVVRHISDRVAVMYLGKVMELANSTELYENPLHPYTKALLSA
                     IPITDPELERTRERIILTGDVPSPLRPPPGCVFNTRCPIAIEECRERVPEWREVVPEH
                     WVACHRV"
     gene            complement(1395..2426)
                     /locus_tag="PLX85_06420"
     CDS             complement(1395..2426)
                     /locus_tag="PLX85_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC62843.1"
                     /translation="MAKLLEVRNLRTYFYTEEGVVRAVDGVSWELEEGETLGLVGESG
                     SGKSVTAMSILRLIPRPPGRIVEGEILFEGQDLLKASDDEIRHIRGNRIGMVFQEPMT
                     SLNPVLTIGAQIGEALQLHLGLDRAQSDARVTELLELVGIPEAGNRIHDFPHQFSGGM
                     RQRVMIAMALACNPKLLIADEPTTALDVTIQAQVLEVMARLSNELGTAVVIITHNLGV
                     VSRYADRVNVMYAGKIVESGATLDIFKDPRHPYTVGLMASVPRLDATEHKRLYAIEGQ
                     PPLLIDPIPGCPFEPRCDWRIEKCKSEAPPLELKAAKHWAACWVNPQVDGRTVIREEV
                     AGAQLATQA"
BASE COUNT          403 a          736 c          935 g          458 t
ORIGIN      
        1 acgtgccccg gcgtgtcgat caggttcagc tggtagcgct gcccgtcctt cgccaggtag
       61 ggcatgcgca cggcctgcgc cttgatggtg atgcccttct cccgctcgag gtccatcgag
      121 tcgaggagct gttcctgcat gtcacgggcg tcgacggcgc cggtgaactc gagcatgcgg
      181 tcggcaagcg tcgacttgcc gtggtcgatg tgggcgatga tggagaagtt gcggatgcga
      241 gaatcaggca cagcaccatc gtacgggtgc acgccgctgg gcgcttggga cagacggaaa
      301 tcgcgcccct gcgggcgcga tttcgtgtga ctaaggctga ccgtccgcta gacgcggtgg
      361 caggcgaccc agtgctcggg gaccacctcg cgccactcgg gaacacgctc gcggcactcc
      421 tcgatggcga tcgggcagcg ggtgttgaac acgcagccgg ggggcgggcg gaggggcgac
      481 ggcacgtcgc cggtgagaat gatgcgctcg cgggtgcgct cgagctcagg gtcggtaatc
      541 gggatggcgg agagcagcgc cttcgtgtac gggtggagcg ggttctcata gagctcggtg
      601 gagtttgcga gctccatgac cttgccgagg tacatgaccg ccacgcggtc ggagatgtgg
      661 cgtacgacgg cgaggtcatg tgcgatgaac aggtacgtga ggttgaactc cgtctggagg
      721 tcttccatca ggttgacgat ctgggcctgg atggagacgt cgagggcgga gactggctcg
      781 tctgcgacga tgaagtcagg ctcgaccgcg agcgcgcggg caataccgat acgctggcgc
      841 tggccgccgg agaactcgtg cgggaagcgc ttggcgaagt acgggttcag cccgaccgca
      901 ttcatgatgt aggagatgcg gtcgcggcgc tcgccgccct tgtgcaggcc gtggatggcc
      961 atcggctcgg cgatgatgct ctgcacgttc atgcgtgggt tcagcgatga gtaggggtcc
     1021 tggaagatca tctgcatgcg acggcggaag gagcggagcg ccttcggctt gagggtggtg
     1081 agctcggtgc cgtcgaagtt caccgatccg ccggagggct tgtagagctg gagcacggcc
     1141 cggccggtcg tggacttgcc gcagccggac tcgccaacga ggccgagcgt ctcacccttg
     1201 cggacgaaga agctcacgtc gtcgacggcc ttgacctcgg ccaccttgcg ctggaccagg
     1261 acgccagcgg tgaccgggaa gtacatcttg aggttgttga cgtcgatcag gcccctgcgc
     1321 tgggtattcg cgccgtcggt cgcctgggag gtcgtggact cggtcgtggt catggccgtt
     1381 tctccctgcg tccgttaggc ctgcgtggcg agctgggcgc cggcgacttc ctcgcggatc
     1441 accgtgcgtc catccacctg cgggttgacc cagcaggcag cccagtgctt ggccgccttc
     1501 agttcgaggg gtggcgcctc agacttgcac ttctcgatgc gccagtcgca gcgaggctcg
     1561 aatgggcacc ccggaatggg gtcgatcagc agcggcggct gaccctcgat cgcgtagagc
     1621 cgcttgtgct cggtggcgtc gaggcggggc acggaggcca tcaggccgac ggtgtagggg
     1681 tggcgagggt ctttgaagat gtcgagcgtc gcgcccgact cgacgatctt gccggcgtac
     1741 atgacgttga cgcggtccgc gtagcgggac acgacgccga ggttgtgagt gatgatcaca
     1801 acggcggtgc cgagctcgtt ggagaggcgt gccatcacct cgaggacctg cgcctggatg
     1861 gtcacatcga gggcggtcgt gggctcgtcg gcgatgagga gcttcgggtt gcaggccagc
     1921 gccatcgcga tcatgacgcg ctggcgcatc ccgcccgaga actggtgcgg gaagtcgtga
     1981 atgcggttgc ccgcctccgg gatgccgacg agctcgagga gctcggtcac acgggcatcg
     2041 gactgagcgc ggtcgaggcc gaggtgaagc tggagggctt cgccgatctg ggcgccgatc
     2101 gtgaggacgg ggttgaggga ggtcatcggc tcctggaaga ccatgccgat gcggttgccg
     2161 cgaatgtggc ggatctcgtc gtcactggcc ttgaggaggt cctggccctc gaagaggatc
     2221 tcgccctcga cgatgcggcc cggcggccgc gggatgagcc ggaggatgga catcgcggtg
     2281 acggacttgc cggacccgga ctcaccgacg agaccgaggg tctcgccttc ctcgagttcc
     2341 cagctgacgc cgtctacagc tcggacgaca ccttcttccg tgtagaagta ggtgcggaga
     2401 tttctaacct caagcagctt tgccattcgc cccccctggc ctgccgacgg ggagagttcc
     2461 aggggtcctc gtcccggtgg tggggaagag gagactcgaa ctcctacgcc ttgcggcaca
     2521 tgatcctaag tc
//
LOCUS       DAORPG010000264         2528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4583_length_2528_cov_4.185200, whole genome shotgun sequence.
ACCESSION   DAORPG010000264 DAORPG010000000
VERSION     DAORPG010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4583_length_2528_cov_4.185200"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..587)
                     /gene="folE"
                     /locus_tag="PLX85_06425"
     CDS             complement(9..587)
                     /gene="folE"
                     /locus_tag="PLX85_06425"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005325658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRC62844.1"
                     /translation="MLPAANAYDVDAAQRIVRDLLIAIGEDPDREGLRETPRRIASMY
                     QELFEGLRQDPVAVLSVGFEEGHDEMVILREIPFYSMCEHHFMPFHGEAHVGYLPDGR
                     IVGLSKIARAVDIFARRPQVQERLTGQIADCIEQVLGAKGVGVVIEAEHLCMTARGVR
                     KPGSKMVTSAMRGRFRTDQNTRQEFLGLIRPD"
     gene            complement(684..896)
                     /locus_tag="PLX85_06430"
     CDS             complement(684..896)
                     /locus_tag="PLX85_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010063153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HRC62845.1"
                     /translation="MPDEKVKGPASYFPSIEKKYGKPIAEWQAIVRASPLTKHMELVN
                     MLKTEHGMGHGHANAIVAYTLADDAS"
     gene            complement(924..1985)
                     /gene="thrC"
                     /locus_tag="PLX85_06435"
     CDS             complement(924..1985)
                     /gene="thrC"
                     /locus_tag="PLX85_06435"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009768199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRC62846.1"
                     /translation="MTPTARFIMERYASLLPVTEHTPLVTLGEGGTPLVRSRSIGPEL
                     GLEALYFKLEYANPTGSFKDRGMVMAVAKAVEEGATSLLCASTGNTSASAAAYAARHG
                     LRAYVVVPSGRIASGKLAQAVAHGAEIVAIDGSFDDALRIAKQLAERPGVALVNSVNP
                     YRIEGQKTAAFEVVDDLGDAPDLLCIPVGNAGNITAYWRGFVEYQRLERASKRPSMWG
                     FQAAGAAPLVLGHPVEKPETIATAIRIGNPASWEGAITARDDSNGVIEAVTDDEILEA
                     YRRLAATEGVFCEPASAASVAGLLKRARAGASLAGLTAVCVITGHGLKDPDTAMTIEA
                     VRHNVPADLAAVEESLGWR"
     gene            2009..>2528
                     /locus_tag="PLX85_06440"
     CDS             2009..>2528
                     /locus_tag="PLX85_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62847.1"
                     /translation="MSALGELAKQLAASAGLTVDLLELAAASVLGGSGGAADMFEEEV
                     TLGLDAGVETGQMRLVGGELATQEVTLLGDQALGFVAGGDGFLDGLKPSLDAFAEAAH
                     LFGGIGDEVGQTGARPQLRDVVIEAALGVLHGREASLERRDLEAQLGEDGRRRGEFMI
                     VAADAGGERFNAA"
BASE COUNT          373 a          743 c         1017 g          395 t
ORIGIN      
        1 ggatagggct agtcggggcg gatgaggccg aggaactcct ggcgggtgtt ctggtcggtg
       61 cggaagcggc cgcgcatcgc ggaggtgacc atcttcgagc cgggcttgcg cacccctcga
      121 gccgtcatgc agaggtgctc cgcctcgatg acgacgccga cgcccttcgc gcccaggacc
      181 tgctcgatgc aatcggcgat ctggccggtg aggcgctcct ggacctgcgg gcggcgggcg
      241 aagatgtcga cggcacgggc gatcttcgag aggccgacga tgcggccgtc cggcaggtag
      301 ccgacgtggg cttcgccgtg gaacggcatg aagtggtgct cacacattga gtagaagggg
      361 atctcgcgga ggatgaccat ctcgtcgtgg ccttcctcga agccgacgga gaggacggcg
      421 acagggtcct gacggagccc ctcgaagagc tcctggtaca ttgaggcgat gcggcggggg
      481 gtctcgcgga gaccctcgcg gtcggggtct tcaccgatag cgatgagaag gtcgcggacg
      541 atgcgctgcg cggcatcgac gtcgtaggcg ttcgcggcgg gcaggatggt ggtcacggca
      601 tcggcctcga ttccgcagga gcgagcgcgt aaacgcgatc gtagcacgcc gcagcgatgg
      661 aaccgtggat ggccagccgg tggctagctc gcgtcgtcgg caagcgtgta ggcgacgatg
      721 gcattcgcat ggccatggcc catgccgtgc tctgtcttga gcatgttgac cagctccatg
      781 tgcttcgtga gcggactcgc gcggacgatc gcctgccact ccgcgattgg cttcccgtac
      841 ttcttctcga tggacgggaa ataggaagcg ggacccttta ccttttcgtc aggcacgcgg
      901 cacctccgaa gcggggggtg agactagcgc cagccgaggc tctcctcgac ggctgcgagg
      961 tcggcgggga cgttgtgacg gaccgcctcg atggtcatgg cggtgtcagg gtccttcagg
     1021 ccatggccgg tgatgacgca gacggcggtg aggcccgcaa ggcttgcgcc ggcgcgagcg
     1081 cgcttgagga ggccggcgac cgaggcggct gaggcgggct cgcagaagac gccctcggtg
     1141 gcggcgaggc ggcgataggc ctcgaggatc tcgtcgtcgg tgacggcctc gatgacgccg
     1201 ttggagtcgt cgcgggcggt gatggcgccc tcccaggagg cggggttgcc gatgcggatg
     1261 gccgtggcga tcgtttcggg cttctcgacg gggtggccga ggacgagcgg cgcggcgccg
     1321 gcggcctgaa agccccacat ggagggccgc ttcgaggcgc gctcgaggcg ttggtactcg
     1381 acgaagccgc gccaataggc ggtgatattg ccggcgttcc cgacggggat gcagaggaga
     1441 tcgggggcgt cgccgaggtc gtcgacgacc tcgaaggcgg cggtcttctg gccctcgatg
     1501 cggtaggggt tgacggagtt gacgagagcg acgccggggc gctcggcgag ctgcttggcg
     1561 atgcggaggg cgtcatcgaa ggaaccgtcg atggcgacga tctcggcgcc gtgggcgacg
     1621 gcctgcgcga gcttgccgga ggcgatgcgg ccgctgggga cgacgacgta ggcgcggagg
     1681 ccgtggcgag cggcgtacgc ggcggccgag gcggacgtat tgccggtgga ggcacagagc
     1741 agcgaggtcg cgccttcctc gacggccttg gcgacggcca tcaccatccc gcggtccttg
     1801 aaggagcccg tggggttggc gtactcgagc ttgaagtaga gcgcctcgag gccgagctcg
     1861 ggaccgatgg agcgcgagcg gacgagcggc gtgccgcctt cgccgagcgt gacgagcggg
     1921 gtgtgctcgg tcacggggag cagcgaagcg tagcgctcca tgatgaagcg ggcggttggg
     1981 gtcatagggg ttgttctcgc gatccttctt gagcgcgctc ggtgagctgg cgaagcagct
     2041 cgcggccagc gcgggcctga cggtcgatct cctggagctg gcggcggcca gcgtcctcgg
     2101 cggcagcggt ggcgcggcgg acatgttcga ggaggaggtc acgctgggcc tcgatgccgg
     2161 cgtcgagacg ggccagatgc gcctcgtggg cggcgagctc gcgacgcagg aggtcacgct
     2221 gctcggtgac caggcgctcg gcttcgtcgc gggaggcgac ggcttcctcg atgggctgaa
     2281 gccgagcctc gacgcgttcg cggaggctgc gcacctgttc ggcggcatcg gcgacgaggt
     2341 cgggcagacc ggcgctcgac cgcagttgcg tgacgtcgtc atcgaggcgg cgctgggtgt
     2401 cctgcacggc cgcgaggcga gcctcgaacg ccgcgacctc gaggcgcagc tcggggagga
     2461 cggcagacgt cgcggcgagt tcatgatcgt cgcggcggac gctggcggcg agcgcttcaa
     2521 tgcggcgg
//
LOCUS       DAORPG010000265         2519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4613_length_2519_cov_3.711039, whole genome shotgun sequence.
ACCESSION   DAORPG010000265 DAORPG010000000
VERSION     DAORPG010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4613_length_2519_cov_3.711039"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLX85_06445"
     CDS             <1..317
                     /locus_tag="PLX85_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62848.1"
                     /translation="LRGDWAPLGRLDGSNELRPGAVTIPVALAYERQSLHGDAARHAA
                     GAARDRAWSMLDRDSRAALVNALHFCHQAASAAIDDLRLPEAWSADLSLLANAVCCEG
                     GE"
     gene            367..687
                     /locus_tag="PLX85_06450"
     CDS             367..687
                     /locus_tag="PLX85_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62849.1"
                     /translation="MSDFADLRDLALKLSHELQSGNIAVGQLPSVQYQTAGLNPFLTE
                     VDNQLVSLHKRHLTSQERDLLTYYFHRNTPVKDPNEVAERVAWIARGPNAFYWGPWMP
                     YQDP"
     gene            731..1996
                     /locus_tag="PLX85_06455"
     CDS             731..1996
                     /locus_tag="PLX85_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRC62850.1"
                     /translation="MTIHAMIEFDIRADDAALEDAIRELIRAGRRDGSFSRIPLGDHT
                     IVSGAFDEDRLEDLAGVERVVRIYDDPELTPFAPNFVNTVVDEAHGDIFSIASALMSP
                     LWTHGHRGEGVRIGICDTGIDHKEKKLALEPTRGWTPSAVFSIGEDVSLVPHGTMVAH
                     DALAMAPLSEVLDIGLLKMPQGTLAGVADWRLSVAMDAYRAVIDGFVMDRREETYGLV
                     LCNSWGMRDPEVRWNIGHYADDPTHPFTRLVVEAIDHGIIVVFAAGNCGSNGGKPYSP
                     RGFSGPGNSIWGANGHPRVITVGAADWNRNWMGYSSEGPSSLAPNMIKPDVCGISQFS
                     GLIDIVGKPYFSYPDAGTSAAAPIVAGGLAVLKGKYPKMTQDVAAAVLRATADHPASV
                     NGAPAADNQYGYGIVNFQKADQLLHQIYP"
     gene            complement(2102..>2519)
                     /locus_tag="PLX85_06460"
     CDS             complement(2102..>2519)
                     /locus_tag="PLX85_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007461837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HRC62851.1"
                     /translation="ARGGRRRAQQARWRAVAPPTRLGAGTGPNEETGVWRGIAGSSGV
                     YRGRVRVIASLEDADQLEPGDVLVCAATAPEWTPYFGVIGALVTGAGGVLTHAAVVAR
                     ELGLPAVVGVTGATSQIPDGSFVIVDGSAGTVRLDT"
BASE COUNT          429 a          946 c          760 g          384 t
ORIGIN      
        1 agcttcgggg ggattgggcc cccctcggcc gcctcgacgg cagtaacgaa ctgcgcccgg
       61 gcgccgtgac aattcccgtc gccctcgcct acgagcggca gtccctgcat ggcgatgccg
      121 cccgccacgc cgctggagcc gcgcgcgatc gcgcctggtc gatgctcgac cgggactcgc
      181 gagcggccct cgtcaatgcg ctgcacttct gccatcaggc cgcatcggcc gccatcgacg
      241 acctccggct ccccgaggct tggtcggccg acttgagctt gctcgccaac gccgtctgct
      301 gcgaaggagg cgaatgagga aaccccttcg tcccacccag acaacgcatg tgaggagtgg
      361 ccagtaatgt cggacttcgc cgacctccga gacctcgcgc tgaagctcag ccacgaattg
      421 cagtcgggaa atatcgccgt ggggcagctg ccctcggttc agtaccagac tgccggcctg
      481 aacccgttct tgacggaggt cgacaaccag ctcgttagcc tgcacaagcg ccatctgacg
      541 tctcaggagc gcgatctgct cacctactac tttcaccgca acaccccggt gaaggatccg
      601 aacgaggtgg cggaacgcgt ggcctggatc gcccgtggac ccaacgcctt ctactggggc
      661 ccctggatgc cgtaccagga cccctaaccc cgtcagctaa ccatgcactg acccgatggg
      721 aggaccccgc atgacaatcc atgccatgat cgaattcgac atccgcgcgg acgacgccgc
      781 cctggaagat gcgatccgag agctaatccg ggcgggtcgc cgcgacggca gcttctctcg
      841 catcccgctg ggggatcaca cgatcgtcag cggcgccttc gacgaagacc gcctggagga
      901 cctcgccggc gtcgagcgcg tcgtgcgcat ctacgacgat cccgagctca cacccttcgc
      961 accgaacttc gtcaacaccg tcgttgacga ggcgcacggc gacatcttca gcatcgcctc
     1021 tgccctcatg agcccgctct ggacgcacgg tcaccgaggc gagggggtgc gcatcgggat
     1081 ctgtgacacc ggcatcgacc acaaggagaa gaagctcgcc ctcgagccga cgcggggatg
     1141 gacgccgtct gccgtcttct cgatcgggga ggatgtgtcg ctcgttccac acggcacgat
     1201 ggtcgctcat gacgcgctcg cgatggcccc gctgtcggag gtgctggaca tcgggctcct
     1261 caagatgccc cagggcacgc tggccggagt cgcggattgg cgactctcag tcgcaatgga
     1321 cgcctaccgt gccgtgatcg acggcttcgt catggatcgt cgagaggaga cctacggtct
     1381 cgtgctctgc aacagctggg gcatgcgcga ccccgaggtg cgatggaaca tcgggcacta
     1441 cgccgacgat ccgacccacc ccttcacccg gctcgtggtg gaggcgatcg atcacgggat
     1501 catcgtggta ttcgccgccg gtaactgtgg ctccaacggt ggcaagccgt acagccctcg
     1561 gggattctcg ggacccggca acagcatctg gggcgcgaac ggtcacccgc gagtcatcac
     1621 cgtcggcgct gctgactgga accggaactg gatgggctac tcgagcgagg ggccatcatc
     1681 cctcgctcca aatatgatca agccggatgt ctgcggtatc agccagttct cgggcctcat
     1741 cgacatcgtc ggcaagccct acttctcgta ccccgacgcc ggcacctcgg ctgccgcacc
     1801 gatcgttgcc ggcggcctcg cggtactgaa gggcaagtac ccgaagatga cccaggacgt
     1861 ggccgcggca gtcctgaggg cgaccgcgga ccacccggcc tcggtcaacg gcgcgcccgc
     1921 cgccgacaac cagtacggct acgggatcgt caacttccag aaggcggacc agctgctgca
     1981 ccagatctac ccgtagagcg gagatcgccg cacctgcccg cccgcctcat gctccgcggc
     2041 tcccgccccc tctgtcgcgg agcatgaggc cgcccccctg ccgccgaggg cccttcagcc
     2101 cctaggtgtc cagccgcacc gtccccgctg agccgtccac gatgacgaag ctcccatccg
     2161 ggatctgcga ggtcgcaccg gtcaccccga ccaccgctgg caaccccagt tcccgggcca
     2221 cgaccgccgc gtgggtcagc acgcccccgg cccccgtcac cagcgcgcca atcaccccga
     2281 agtacggcgt ccactctggc gcggtcgccg cgcacacgag cacatccccg ggctcgagct
     2341 ggtccgcatc ctcgagtgac gcgatcacac gcacgcgccc tcggtacacc ccgctcgacc
     2401 ccgcaatccc gcgccagacg cccgtctcct cattcggccc ggtacctgcc cccagccgcg
     2461 tcggaggagc caccgcgcgc caccgcgcct gctgcgcccg ccgccgcccg ccccgcgcc
//
LOCUS       DAORPG010000266         2504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4658_length_2504_cov_2.705186, whole genome shotgun sequence.
ACCESSION   DAORPG010000266 DAORPG010000000
VERSION     DAORPG010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4658_length_2504_cov_2.705186"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /locus_tag="PLX85_06465"
     CDS             <1..1421
                     /locus_tag="PLX85_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62852.1"
                     /translation="LGAFGWLGRWSASGAGAKALAAFAAAAVVGAFLLALAMLARHDD
                     ASLGKLVWVRDGDLWVRTLPDGEPRRLTSDGGNSLPRWSPSGEWVLYRHGQLGVDAES
                     RVMRADGSEDRRLDVSEGFYAGQWKPGEDRLLLVRPDGAVVVEDVATGSALTILEAFA
                     STTEGRAVLPVWSLDGARVVFFELRWPAEAGSRGLPARAKTYSGLSVIDSDGTNRREL
                     VNLGANPTKQIGPLGFAGERLLFMEMPEDAGESIFDGVELLSVSLSGGAVQDLGGRVT
                     LLSGMANLFGERAFVVLGDERESWTGKRIALVDATTGQVRSLTGTDVAALSPQVSADG
                     ARVAYVAAPDLGAGVRDRALASRRIWVMDADGSNARQVSRDEEYRHEAPRWSNDGKYM
                     LFARLTLERCSPSWTLQLLDVRDGSIREVASELPAISTHNERPVIDQIPECDPGGKPG
                     SISDTYGSVGVTQAYDWWQPAG"
     gene            complement(1511..1777)
                     /locus_tag="PLX85_06470"
     CDS             complement(1511..1777)
                     /locus_tag="PLX85_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62853.1"
                     /translation="MVREQERPQRPGLEPETRELEPMQRPDDMPELPDEERQLGQQEP
                     EPTPREWTEPRPGGDASPAPGSRIDPTTEERGPHLTDEDIGEAP"
     gene            complement(1889..>2504)
                     /locus_tag="PLX85_06475"
     CDS             complement(1889..>2504)
                     /locus_tag="PLX85_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006901139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRC62854.1"
                     /translation="LLGAAFVAYLRFVRPRVLDWGAREDERRRTLPGDDILAAPAIQT
                     TRAVTIEAPPDRIWPWLVQMGPRPRAGVYTYDWIERRLGIDIENSDRLLPEFQHLEAG
                     TFWELSPGQGLRVREVEPGRAIVLQWEPTESTWAFVLASVPGDEGATRLISRNRVQGH
                     GLRFWLLMRLLMEPGSLVMERKMLLGIRDRAERTTAEDIAAITH"
BASE COUNT          325 a          701 c         1081 g          397 t
ORIGIN      
        1 gcctcggggc gttcgggtgg ctcgggcggt ggtcggcgtc aggggcgggc gcgaaggcgt
       61 tggcggcatt cgcggcggcg gccgtggtgg gcgcattcct gctggcgctc gcgatgttgg
      121 cgaggcacga cgacgcgagc ctcgggaagc tggtctgggt gcgggacggg gacctgtggg
      181 tgcggacgtt gccggacggg gagccgcggc ggctgacctc ggacggcggg aactcgctgc
      241 cgcggtggtc gccgagcggg gaatgggtgc tgtaccggca cggtcagttg ggcgtggacg
      301 cggagtcgcg cgtcatgcgc gccgacgggt ccgaggaccg gcggctcgac gtgtcggagg
      361 gcttctacgc ggggcagtgg aagccgggag aggaccgact gctccttgtg cgtccggacg
      421 gcgcggtggt cgtcgaggac gtggcaacgg ggtcagcgct cacaattctc gaggccttcg
      481 ccagcacgac tgaggggcgg gcggtgctgc ccgtgtggtc tctcgatggc gcgcgggtgg
      541 tgttcttcga actgcggtgg ccggcggagg cgggctcgcg tgggttgccc gcacgggcga
      601 agacgtactc cggactcagt gtgatcgatt ccgacgggac gaaccggcgt gagctggtaa
      661 acctgggcgc gaacccgacg aagcagatcg ggccgctggg cttcgcgggt gaacgcctgc
      721 tgttcatgga gatgcccgag gacgctgggg agtcgatctt cgacggtgtc gagctgttgt
      781 cggtgtcgct gtcgggaggg gccgttcagg acctcggtgg gcgggtgacg ttgctgtcgg
      841 gcatggcgaa cttattcgga gagcgcgcct tcgtcgtcct cggcgacgag cgcgagtcgt
      901 ggacggggaa gcgcatcgcg ctggtcgatg ccacgaccgg acaggtcagg tcactcaccg
      961 gtacggacgt ggcggcgctg tcgcctcagg tgtcggcgga cggggcgcgg gtcgcgtacg
     1021 tggcggcgcc tgacctcggc gcgggcgtgc gggaccgggc gctcgcgtcc cggcggatct
     1081 gggtgatgga tgcggacggg tcgaacgcgc ggcaggtgtc gcgggacgag gagtaccggc
     1141 acgaggcgcc tcgatggtcg aacgatggaa agtacatgct gtttgcgcgg ctgacgctcg
     1201 agcggtgttc gccctcgtgg acgttgcagt tgctcgatgt gcgggatggc tccattcggg
     1261 aggtggcctc ggagttgccg gcaatctcga cccacaacga gcggccggtg atcgaccaga
     1321 tccccgagtg cgatccgggc gggaaaccgg ggagcatcag cgacacctat gggagtgtgg
     1381 gcgtgacgca ggcgtacgac tggtggcagc cggcagggtg agtcgggggc gacggggcgt
     1441 atacggccac ccgggcggcg cgggtgcgcg gcccgggtga cgatatcacg gtcgaacgtg
     1501 tgctctggcc ctacggggcc tcgccgatgt cctcgtcggt gaggtgaggg ccgcgttcct
     1561 cggtggtcgg gtcgatgcga gaaccggggg ccggcgaggc gtcgccgccg gggcgcggct
     1621 cagtccactc gcgaggggtg ggttcgggct cctgctgccc gagctgacgc tcctcgtcgg
     1681 ggagctcagg catatcgtcg gggcgctgca tcggctcgag ctcgcgggtc tcgggctcga
     1741 ggccggggcg ctgcgggcgt tcctgttcgc ggaccattga ggttcctcct cgcggtgcgg
     1801 tgggttgtgg aaggaggtta gggagcgggc cgctacggcc gggtgacctc gatggcgggc
     1861 gagtggcgat tgggttgcag gggggcgatc agtgcgtgat ggccgcgatg tcctcggctg
     1921 tggtgcgctc ggcgcggtcg cggatgccga ggagcatttt gcgctccatg acgagggagc
     1981 cgggctccat gaggaggcgc atgaggagcc agaagcggag gccgtggccc tggacgcggt
     2041 tgcgggagat gagacgcgtt gcgccctcgt cgccggggac ggaggcgagg acgaaggccc
     2101 aggtggactc ggtgggctcc cactggagga cgatggcgcg gccgggttcg acctcgcgga
     2161 cgcggaggcc ctggcccggg ctgagctccc agaacgtgcc ggcctcgagg tgctggaact
     2221 cggggaggag gcggtcgctg ttctcgatgt cgatgccgag gcggcgctcg atccagtcgt
     2281 aggtgtagac gccggcacga gggcgggggc ccatctggac gagccacggc cagatgcggt
     2341 ccggcggcgc ctcgatggtg acggcgcggg tggtctggat cgcaggggcc gcgaggatgt
     2401 cgtcgccggg gagggtgcgg cggcgttcgt cctcgcgggc gccccagtcg agaacgcgag
     2461 ggcgcacgaa gcggaggtag gcgacgaaag cggcgccgag gagg
//
LOCUS       DAORPG010000267         2503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4660_length_2503_cov_3.943627, whole genome shotgun sequence.
ACCESSION   DAORPG010000267 DAORPG010000000
VERSION     DAORPG010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4660_length_2503_cov_3.943627"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PLX85_06480"
     CDS             <1..426
                     /locus_tag="PLX85_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62855.1"
                     /translation="SFQQEGALFGMSVDVEVSVFKDSKVPASLISDAKGAIGSADFEN
                     CFREILKSSGDGIPSDTKFELKSAKPLASAPNNGISQAYDLSISSSGISFSMHAELYG
                     WANKNASALVTIFGTPDDLKPEAVKAAIAKTDEKLSKAQ"
     gene            complement(496..987)
                     /locus_tag="PLX85_06485"
     CDS             complement(496..987)
                     /locus_tag="PLX85_06485"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HRC62856.1"
                     /translation="MIVLIMGVAGSGKTTAGTAVAARLGWRFVDADDYHFAANLAMLR
                     AGLPLTDGDRRPWLERLAVVIAEARQHEESLALACSALKRSYRDLILGRERGDVLLVH
                     LVGDPALIASRMSARTHFMPPSSLPQQLATLEPPTADERPLTLDAAVPLDALVDRIFA
                     AAT"
     gene            1100..>2503
                     /locus_tag="PLX85_06490"
     CDS             1100..>2503
                     /locus_tag="PLX85_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HRC62857.1"
                     /translation="MHPSSSDSASATAEQPRTSRPLSSEELWQLDAWWRAANYLSVGQ
                     IYLLDNPLLREPLRTQHIKPRLLGHWGTTPGLNFLYAHINREIGRRDLEAMYVIGPGH
                     GGPAAVANAYLEGSFSEAYPHVSRDEEGMRQLFREFSFPGGIGSHVTPEVPGSIHEGG
                     ELGYSLAHAFGAAFDNPRLTVFCVVGDGEAETGALATSWHSNKFLNPATDGAVLPILH
                     LNGYKIASPALLARIPHDELEALLEGYGYRPYWVEGDDPAEVHQSLAATLDSALDEIA
                     RIQRAAREQGQYGRPRWPAIVFRTPKGWTGPHEVDGQLTEGTFRSHQVPLADIGSKPG
                     HLALLEAWMRSYRPQELFDEAGTLRPEIAALAPRGERRMSASPHANGGLLVRELRLPE
                     FDHYAVAVPSPATTFSEATRVLGGYLRDVIRENPETFRLFGPDETASNRLTPVFEATN
                     RAWEGPVLASDDHLAPNG"
BASE COUNT          431 a          864 c          822 g          386 t
ORIGIN      
        1 agcttccagc aggaaggcgc cctcttcgga atgtccgtgg atgtcgaggt gtccgtcttc
       61 aaggactcga aagtgccggc atcgcttatt tcggacgcca agggcgccat cgggtcagcc
      121 gacttcgaga actgcttccg ggagatcctg aagagttccg gcgacgggat cccttccgac
      181 accaagttcg aactcaagtc ggcaaagccg ctggcctcgg cgccgaacaa cgggatctcg
      241 caggcctacg acctcagcat ctcctcgtcg ggcattagct tctcgatgca cgccgagttg
      301 tacggctggg cgaacaagaa cgccagtgcg ctcgtcacca tcttcggcac gccggacgac
      361 ctcaagccgg aggccgtgaa agccgccatc gcgaagacgg acgagaagct ttcgaaggct
      421 caataggtcg agcgccacag acgcacgaac gagagaggga ggccgcaggg gcctccctct
      481 ctcgttgggc ctcgctcacg tggctgcggc gaagatccga tcgaccagtg catcgagcgg
      541 cacggcggcg tcgagcgtca acggtcgctc gtccgcagtc ggcggctcga gcgtggcgag
      601 ttgctggggc aggctcgacg gcggcatgaa gtgcgtccgc gcgctcattc gggacgcgat
      661 cagcgcgggg tcgccgacca ggtggacgag cagtacgtct ccccgctcgc ggccgaggat
      721 caggtcacga tacgaccgtt tcagcgcgga gcacgcgagg gcgagcgact cttcgtgttg
      781 ccgcgcctcc gcgatcacca cggcgaggcg ctccagccat ggacgccggt ctccatcggt
      841 gagcggcaat cccgcacgca acatcgcgag gttcgcggcg aagtggtagt cgtcggcatc
      901 gacgaaccgc cagccgaggc gagcggccac ggccgtgccc gcggtcgtct tgccggaacc
      961 cgcgacgccc atgatgagca cgatcacttc agacccatct cgtcgacctg aggcgtggac
     1021 aggtagctca ctcttaacac tgtccctcga tcatggatcg ggacgagcca gttgcgagtg
     1081 tctgaagccg gaggcggcga tgcatccatc tagttcggac agcgcgagcg ccacagccga
     1141 gcagccgcgc acctcgcgcc ctctttcgag cgaggagctg tggcaactcg atgcctggtg
     1201 gcgagccgcc aactacctct cggtcggcca gatctacctg ctcgacaatc ccctgctgcg
     1261 tgagccgctg cgcacccagc acatcaagcc ccgcctcctc ggccactggg ggacgacccc
     1321 cgggctcaac ttcctgtatg cgcacatcaa tcgcgagatc ggccgccgcg acctcgaggc
     1381 gatgtacgtc atcggtccgg gacatggtgg tccggccgcc gtcgccaacg cgtacctcga
     1441 agggagcttc agcgaagcgt atccccatgt ctcgcgcgac gaggaaggca tgcgccagct
     1501 gttccgcgag ttctcgttcc ccggaggcat cggcagccac gtcacccccg aggttcccgg
     1561 gtcgatccac gagggcggtg agctgggcta ctcgctggcg cacgccttcg gggcggcgtt
     1621 cgacaatcct cggctcaccg tcttctgcgt ggtgggcgac ggcgaggcgg agacgggcgc
     1681 gctggcgacg agctggcact ccaacaagtt cctgaacccg gccacggacg gcgcggtgct
     1741 gcccatcctg cacctcaacg gctacaagat cgcgagcccc gcgctgctcg cccgcattcc
     1801 ccatgacgag ctcgaagcgc tgctcgaggg ctacgggtat cggccgtact gggttgaggg
     1861 ggacgaccct gccgaggtcc accagtcgct tgccgccacg ctcgacagcg cactggacga
     1921 gattgcgcgg attcagcggg ccgcacgtga gcaaggccag tacggccgtc cacgctggcc
     1981 cgcgatcgta tttcgcacgc cgaagggctg gaccggcccg cacgaggtcg acggtcaact
     2041 gacggagggc acgtttcgct cgcatcaggt accgcttgcc gacattggca gcaaaccggg
     2101 gcacctggcg ctgctcgaag cctggatgcg gagttaccgg ccgcaggagt tgttcgacga
     2161 ggccgggacg ctccgtccgg agatcgccgc gctcgcgcct cgaggtgagc gccgcatgag
     2221 cgccagcccg cacgccaacg gcggcctgct cgtgcgagag ctgcgcctgc ccgagttcga
     2281 ccactatgcc gtcgccgtgc cctcgccggc gacgaccttc tccgaagcga cgcgcgtcct
     2341 cggcggctac ctccgggacg taatccgcga gaaccccgag acgttccggc tcttcggccc
     2401 ggacgagacg gcctcgaacc ggctcacccc ggtattcgag gcgacgaacc gggcgtggga
     2461 aggccccgtc ctcgcctcgg acgaccacct ggcgccgaac ggc
//
LOCUS       DAORPG010000268         2496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4675_length_2496_cov_3.714461, whole genome shotgun sequence.
ACCESSION   DAORPG010000268 DAORPG010000000
VERSION     DAORPG010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4675_length_2496_cov_3.714461"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1651
                     /locus_tag="PLX85_06495"
     CDS             <1..1651
                     /locus_tag="PLX85_06495"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC62858.1"
                     /translation="SSVAVGWQGDRRKFLGLTGSVAAAGLVLSTSCFGGGSDESTPTS
                     TAAASETAAATRRGGTLRMGQSGDLSLSAGHPFALVSQNRILFYAVSETLVGYGASLT
                     PVPVLAERFEVSADFRRVSATLKAGVQFHNGAPVTTDDVAFSVQTLQNPASAGLPGAL
                     QFELTPVARSIADIRVVDARTIEFTFDSPRTNAADFFTQLPIAQRTSFKEIAQGNVVG
                     SGPFQLKQWDRNRGYRLERFANWHGAGSDGKPYLDAIEVSIVPDQTAAMIAFRGGNLD
                     AYLAMSAPNAVQLRGSGQTRLTGKTGMSYLGVNVTNPLVQDRRVRQAIFYAVNRDRMV
                     RDAGQGFGSVTTQPWASTSPAFDPGREAPFYDLPRARALLSQAGFQQDRELAIEYPAG
                     IPLQELQAQLLQSDLKAAGIDTRLEAIDGARFTQRYRAREFPDFWLASHLSGDFTPLT
                     LLQQAFEFRATGNASSFEDSAYAALVRRLETLAPASADAKDVYRQINQLMLENPFVIP
                     TGIPQVRIDLVQDSVLGWPAAPADYSIAPSGKVDFARISLR"
     gene            1734..>2496
                     /locus_tag="PLX85_06500"
     CDS             1734..>2496
                     /locus_tag="PLX85_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRC62859.1"
                     /translation="MPDRVIEGEGTRIEEHGPIAVVKLNRPERMNAFGDEMTTEINSY
                     LYELNEGDYRIRAIILTGEGRAFCAGGNVKNFPGADLAKQRPPWRPSHHEQYTVSRLR
                     YCDVPVIAAINGFCLGMGLGLALGTDLRICADDAQFQVAQIKRGIVGDYGVPYLLPRT
                     VGMQRAMELMMTGRRFSAEEAKAYGIVLDIVPAAELMDRAFDLATMIAKGPPLGLAAT
                     KRLAYQNEFDELARVQDLTGQFVQHLFRSEDGVEGV"
BASE COUNT          432 a          831 c          831 g          402 t
ORIGIN      
        1 ctcctcggtc gccgtgggat ggcaagggga tcggcgcaag ttcctgggtc tgaccggcag
       61 cgttgcggca gccggcctgg tgctctcgac cagctgcttc ggtggcgggt ctgacgagtc
      121 gacgcccacg tcgaccgccg ccgcctcgga gacggcggcg gccacacggc ggggcggcac
      181 gctcaggatg ggccaaagcg gcgaccttag cctcagcgca ggacacccct tcgcgctggt
      241 gtcgcagaac cgaatcctgt tctacgcggt gagcgagacg ctggtggggt atggcgccag
      301 cctgacgccc gtccccgtgc ttgccgagcg attcgaggtc agcgccgact tccggcgcgt
      361 ctcggcgaca ctcaaggctg gcgtgcaatt tcacaacggg gccccggtca cgacggacga
      421 cgtggcgttc agcgtgcaga ccctgcagaa cccggcgtcc gccgggctgc ccggggcact
      481 gcaattcgag ctgaccccgg tcgcgcggtc catcgccgac atccgggtcg tggacgcccg
      541 aacgatcgaa ttcacgttcg attcgccgag gacaaacgcc gccgacttct ttacgcagct
      601 cccgattgcc cagcgtacga gcttcaagga gatcgcacag ggtaatgtcg tcgggagtgg
      661 gcccttccag ctgaagcagt gggaccgcaa ccgcggttac cggcttgagc gctttgccaa
      721 ctggcacggg gcgggcagtg acgggaagcc atacctcgac gccatcgagg tgtcgatcgt
      781 ccccgaccag acggcagcca tgatcgcgtt ccggggcggc aatctcgacg cctacctcgc
      841 catgagcgca cccaatgccg tacagctacg cggcagcggt cagacgcgcc tcacgggcaa
      901 gacgggcatg agctacctgg gcgtgaacgt cacgaacccg ctcgtgcagg accgccgcgt
      961 gcgacaggcg atcttctatg ccgtgaaccg cgaccgcatg gtccgagatg ccggacaggg
     1021 ctttgggtcg gttacgactc agccgtgggc ctcgacctcc ccggccttcg acccaggccg
     1081 ggaagccccg ttctacgacc tgccgcgggc gcgcgcgctg ctgtcgcagg cggggttcca
     1141 gcaggaccgc gaactcgcga tcgagtaccc ggccggtatc cccctgcagg agctgcaggc
     1201 gcagctgttg cagtccgacc tgaaggcagc gggcatcgac acccgcctcg aggccatcga
     1261 cggggcgcga tttacgcagc ggtaccgtgc gcgcgagttc ccggacttct ggcttgcttc
     1321 gcacctctca ggtgacttca ctcccctcac gctgcttcaa caggcatttg agttccgcgc
     1381 taccggcaac gcgagctcgt tcgaggattc ggcatacgcg gccctcgtac ggcgtctcga
     1441 gacactggcg cccgcctccg cggatgccaa ggatgtctac cggcagatca accagctgat
     1501 gctggagaac ccgttcgtga tcccaaccgg tatcccgcaa gtacggatcg acctcgtcca
     1561 ggacagcgtc ctcggctggc cggcagcgcc ggcggactac tccattgcgc catcgggcaa
     1621 ggtggacttc gcccgcatct cgctgcgcta agggtcgcgt tcgcggcagt tgccagcccg
     1681 catatcatct gttatccact ggactcgggc gggcgtgcgc gacggaggct gacgtgccag
     1741 acagagtgat cgagggtgag ggcacacgga ttgaggagca cggcccgatc gccgtggtga
     1801 aactcaaccg gcctgagcgc atgaacgcgt tcggcgacga gatgactacc gaaatcaact
     1861 cgtacctcta cgaactgaac gagggtgact accggatccg ggcgatcatc ctcacaggcg
     1921 agggacgggc cttctgcgct ggcggaaatg tgaagaactt cccaggcgct gacctcgcca
     1981 agcagcgacc gccctggcgc ccgagccacc acgagcaata cacggtcagc cgcctgcggt
     2041 actgcgacgt gcccgtcatt gccgcgatca acggcttctg cctcggaatg gggttgggcc
     2101 tcgccctggg gactgacctg cgaatctgtg ccgacgacgc gcagttccag gtggcgcaga
     2161 tcaagcgcgg gatcgtcggc gactatggcg ttccctatct gctgccgcgc acagtgggca
     2221 tgcagcgggc gatggaactg atgatgacgg gccgccgctt cagtgcggag gaggccaagg
     2281 cgtacggaat cgtcctcgac atcgtcccgg cggcggagct gatggaccgc gcgttcgacc
     2341 tggcgacgat gatcgccaag gggccaccgc tcggcctcgc ggcgacgaag cgcctcgcgt
     2401 accagaacga gttcgacgaa ctcgcgcggg tgcaggatct gacggggcag ttcgtgcagc
     2461 acctcttccg ctcggaagac ggcgtcgagg gcgtga
//
LOCUS       DAORPG010000269         2494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4684_length_2494_cov_4.463305, whole genome shotgun sequence.
ACCESSION   DAORPG010000269 DAORPG010000000
VERSION     DAORPG010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4684_length_2494_cov_4.463305"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLX85_06505"
     CDS             complement(<1..283)
                     /locus_tag="PLX85_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC62860.1"
                     /translation="MYDPVEAARSLQPSIREAADRIEELRRLPPDLVEAMRSAGLFHM
                     LIPQSIGGKEADPVAAARAVEEVAYADGSAGWCVMLAAQSTGFAGLMPEA"
     gene            complement(340..2070)
                     /locus_tag="PLX85_06510"
     CDS             complement(340..2070)
                     /locus_tag="PLX85_06510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRC62861.1"
                     /translation="MRQIGEPPAVPSEQAAVEEVRLFPADHLYLLGVVAAIAIGIGIR
                     AAHLFATNFPLNDGGLFYEMARNLQSDAFKLPETTSYNFADLPFTYPPLGLYLAALLD
                     KLTPFSLVDLFRLLPLLFTSLSIVAFYLLAKRILPSRVAVVAATFAFALVPRSFIWLL
                     MGGGVTRGLGLLMAILALHEMHRTFTTFRMRYAFSAAVFSGLTILSHLETGWFLAFSI
                     AIFFLWFGKERHSVTSSVVVGGSTLLVIAPWMLAVLSAHGTGPFFSANSSGGSVFSGG
                     SVTEYALTSLVRVISTSEPYFPLIGMLGVVGLLSAIKHRLIALPVWWAATILLDVRAF
                     PTFTTIPIAMLAGMAVSDVVIPMLDRGREQWPQLALVRGSNGDTTAPHAQFVAWPGWT
                     TVAVLAALVLYALVGAMLRKPGLGGEGEYLVSLTSGQRVAMHWVDVRTPPDSSFLVVP
                     RSAWQVDKESEWFPVLANRPSVATVQGTEWLPDHGFDDAVSAYDRAWDCGYHTSRCLG
                     LWAESTGKSFDYVYLTRNDRGQCCGTLLESLERDSDYQKVYDGPGGTVFKVVGILPKS
                     TDPQTDDSNS"
BASE COUNT          445 a          838 c          851 g          360 t
ORIGIN      
        1 ctgcctccgg catgaggccg gcgaagcccg tcgactgggc ggcgagcatc acgcaccagc
       61 cagccgagcc gtccgcgtac gccacctcct cgacagcgcg cgcggcggca acgggatcgg
      121 cctctttgcc gccaatcgac tgcggaatga gcatgtgaaa caagccggct gagcgcatcg
      181 cctcgacaag gtcgggaggc agccgtcgca gctcctcgat gcggtctgcc gcctcgcgga
      241 tgctcggctg cagcgaccgc gctgcttcca cggggtcgta catggtctgc tcctcgtacg
      301 cgttcacgcg ggaacgtacg ggagtgtagc cccgcctcct tacgagttgc tgtcgtccgt
      361 ctgcgggtca gtgctcttcg ggagaatgcc gacgaccttg aagacggtcc cgcccggccc
      421 gtcgtacacc ttctggtagt ccgagtcccg ttcgagcgac tcgaggagcg tgccgcaaca
      481 ctggcctcgg tcgttccgag tcaggtagac gtagtcgaag gacttgcccg tcgactcggc
      541 ccacagcccc aggcacctcg aggtgtggta gccgcagtcc caggcacggt cgtacgcgga
      601 gacggcgtcg tcgaagccat ggtcgggcag ccactcggtc ccttgcacgg tggccacgct
      661 ggggcggttc gccagcacag ggaaccactc ggactccttg tcgacctgcc aggcagaccg
      721 gggcacgacg aggaatgagc tgtccggtgg ggtccgcacg tcgacccagt gcatcgcaac
      781 ccgttgcccc gacgtgagac tgacaaggta ttcaccctcg ccgccaagcc ccggcttccg
      841 cagcatcgct cccacgaggg cgtagagcac cagcgccgcc agcaccgcga ccgtggtcca
      901 cccgggccag gcgacgaact gtgcgtgagg cgccgtggtg tcgccgttgc tgccccgaac
      961 cagcgccagc tgaggccact gctcgcggcc gcggtcgagc atggggatca cgacgtcact
     1021 gacggccatg ccggcgagca tcgcgatggg gatcgtggtg aacgtcggga aagcccgcac
     1081 atcgaggagg atcgtggcgg cccaccagac ggggagcgcg atgaggcggt gcttaatcgc
     1141 cgagagcagc ccgaccacgc ccagcatgcc gatgagtggg aagtacggct cactggtcga
     1201 gatcacccgc accagcgagg tcagggcgta ctcggtgacg ctgccgcccg agaagacgga
     1261 gccgcccgag ctattggccg agaagaacgg cccggtcccg tgtgcgctca gcacggccag
     1321 catccagggg gcgatcacga gcagagtcga gccccccacg accaccgagc tggtgaccga
     1381 atgccgctcc ttgccgaacc agaggaagaa gatcgcgatg ctgaacgcga ggaaccaacc
     1441 ggtctcgagg tggctcagga tcgtgaggcc gctgaacacc gcggcgctga aggcgtaccg
     1501 catgcggaag gtggtgaacg tgcggtgcat ctcgtggagc gcaaggatcg ccatcagcag
     1561 tccgagaccg cgagtcacgc cgccgcccat gagcagccag atgaagctcc tcggcacgag
     1621 ggcgaacgcg aaggtcgccg ccacgacggc cacgcgcgag ggcaggatgc gcttcgcgag
     1681 gaggtagaac gcgacgatcg agagcgacgt gaagagcagc ggcagcagcc ggaacaggtc
     1741 gacgaggctg aagggggtca gcttgtccag cagcgcggcc agatagagcc cgagcggcgg
     1801 atacgtgaac ggcagatccg cgaagttgta ggaggtcgtc tcgggaagct tgaacgcgtc
     1861 cgactgcagg ttgcgcgcca tctcgtagaa cagcccgccg tcgttgagcg ggaagttggt
     1921 ggcgaagagg tgggcggcgc ggatgccgat gccgatggcg atcgccgcga cgacgccgag
     1981 caggtacagg tgatccgccg ggaacagccg aacttcctcg acagccgcct gttcggaggg
     2041 tacggccggc ggctcaccga tctgacgcat gaacgtgcag tgtccttccg ctccgggggt
     2101 ggtcaccggg cgcgaggggg cgcagcgcga ggcccctcga acgttgccag ctacgcggga
     2161 gcggctgcat gtaccctacc tgccccctgc actacgcgcc tcagggcgac gcgcccgcag
     2221 gcggcattca atcacgcgat tccatcaccc tcaatgaatc gcctcggcag gcgcgcgcgg
     2281 ataccatagc gcccgtgcat gagccgtcga caccacgatg accgctcccg ccgagggctc
     2341 agctccgtcg ttcgagtctg gcgccccgcc cgagggcttc tcgcgccgct cgcgcttccc
     2401 ggtcgccctt tacgcggcca ccacgttcct cagcgccttc ctcctgttcg aggtccagcc
     2461 gctgatcgcg aagtacatcc tgccgtggtt cgga
//
LOCUS       DAORPG010000270         2492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4691_length_2492_cov_3.167009, whole genome shotgun sequence.
ACCESSION   DAORPG010000270 DAORPG010000000
VERSION     DAORPG010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4691_length_2492_cov_3.167009"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..979)
                     /locus_tag="PLX85_06515"
     CDS             complement(29..979)
                     /locus_tag="PLX85_06515"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRC62862.1"
                     /translation="MLRGAVGIAISVGSMWLLLRAVDGQASLDILRGADRTLVGLAVT
                     CLFVSLAAKIARWRALLPPRGTSFPRLFGIVHISMFLNNVLPLRVGDAVRAGMTVRSQ
                     GVRLTHVVSSMVAERVLDAATLIVCFLLVTPFLPYSPLRLDVTRYLVPTAAAGALVML
                     AGAGLLVLRRRAEGLWSRLGPVLSAVGDSWRRVLSTEGWTIWGWSALAWATAFAINFV
                     LFRALHMEVSPAVAIVLGCSTNLAMLVPSSPAQIGVYHAAATLTLVAFGVDRSVAVSF
                     SVLSHLVNVVPVSLVGAAFLVAELTVRGPRRAAGEASEAP"
     gene            1080..1997
                     /locus_tag="PLX85_06520"
     CDS             1080..1997
                     /locus_tag="PLX85_06520"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRC62863.1"
                     /translation="MLSLTSPRPAEVAPRALHLLQWALLAPLALSLATRTWYQHEWMA
                     WLLLAATTAIWAPDLRRGERRWWFFYVAGVFAYTLLRADADGFLFPVQVDYVIRFDET
                     VFRGHEPVVWLQSQFFSPSSIGIFDVIATQVHWSFFVVPHALAAVIYIWRRPLFARYV
                     IMLLAVEYIGLVCFYLVPTAPPWLASQYGQIGPVYRVMEFVGGSVNADTYQTLYSALG
                     EPNSVAAVPSIHMAVTFAAYLWLRRFMPAASPLFLAYSVVMGISLMLLAEHYFFDLLV
                     GVLVAIAVDLAVARLAGPVEAVPAPARVD"
     gene            2286..>2492
                     /locus_tag="PLX85_06525"
     CDS             2286..>2492
                     /locus_tag="PLX85_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRC62864.1"
                     /translation="MTQQTFVLDRDRAVVVIMDFQQGIVGTVARDPEGTVRRAASVLA
                     AARAASVPVVHVVHRGGRFEAESPD"
BASE COUNT          357 a          963 c          784 g          388 t
ORIGIN      
        1 gagcctaccg gcgcctgcgc tcggacacct agggggcctc cgaggcctcg cccgccgcgc
       61 gccgcggccc gcgcaccgtc agttcggcca ccaggaacgc cgcacccacc aggctcacgg
      121 gcaccacgtt cacgaggtgt gacagcaccg agaagctcac ggccacgctg cggtcgacgc
      181 cgaacgccac cagcgtcagc gtcgccgccg cgtggtagac cccgatctgt gcgggcgacg
      241 agggcaccag catcgccagg ttcgtgctgc atcccagcac gatcgccacc gctggtgaca
      301 cctccatgtg caacgcgcgg aacagcacga agttgatcgc gaacgccgtc gcccacgcca
      361 aggccgacca tccccagatc gtccagccct cggtcgacag cacgcgccgc cagctgtccc
      421 ccacggccga gagcaccgga ccgaggcggc tccacagccc ctccgcgcgt cgccgcagga
      481 cgagcagccc tgccccggcc agcatcacga gggcgccggc cgccgccgtc ggcacgaggt
      541 agcgcgtgac gtcgaggcgc aacgggctgt acggcaggaa cggtgtcacc agcaggaagc
      601 agacgatcag cgtggccgcg tcgagcaccc gctccgccac catcgacgac acgacgtgcg
      661 tcagccgcac cccctggctg cgcaccgtca tccccgcgcg caccgcgtcc ccgactcgca
      721 gcggcagcac gttgttgagg aacatgctga tgtgcacgat gccaaacagc cgcgggaacg
      781 acgtacctcg tggcggcaac aacgcccgcc agcgtgcgat cttcgccgcc agcgacacga
      841 acaggcaggt caccgccagc cccacgagcg tgcggtccgc cccgcgcagg atgtcgaggc
      901 tcgcctggcc gtccaccgcg cgcagcagca gccacatcga gccaactgag atcgcgatgc
      961 ccactgcgcc ccgcagcacc gagccccgcc tgagcagggg agcgctgcga gcggagcccg
     1021 ggccgggcag gaaggtcatt cggctagtcc tgacctgtct tcacgcggta gtgtaagtca
     1081 tgctctcttt gacctcgccc cgtccggccg aggtggctcc tcgcgcgctg cacctgctgc
     1141 agtgggcgct gctcgccccc ctggcgctct cgctcgcgac ccgcacctgg taccagcacg
     1201 agtggatggc ctggctcctc ctcgctgcta cgaccgccat ctgggccccc gacctccgcc
     1261 ggggcgagcg gcgctggtgg ttcttctacg tcgccggcgt cttcgcctac accctcctcc
     1321 gcgccgatgc cgacggcttc ctcttccccg tgcaggtcga ctacgtcatc cgcttcgacg
     1381 agacggtctt ccgaggtcat gagcccgtcg tctggctcca gtcacagttc ttctcgccct
     1441 cgagcatcgg catcttcgac gtcatcgcga cccaggtgca ctggtcgttc ttcgtcgtgc
     1501 cgcatgcgct ggccgccgtc atctacatct ggcgtcggcc gctcttcgcg cgctacgtga
     1561 tcatgctgct cgccgtcgag tacatcgggc tcgtctgctt ctacctcgtc cccaccgccc
     1621 cgccctggct cgcctcgcag tacggccaga tcggccccgt ctaccgggtc atggagttcg
     1681 tcggcggctc ggtgaacgcc gacacctacc agaccctcta ctcagcgctc ggcgagccga
     1741 actccgtggc ggccgtgcca tcgattcaca tggcggtcac attcgccgcc tacctctggc
     1801 tgcggcgctt catgcccgcg gcttcgccgc tgttcctggc ttactccgtc gtcatgggca
     1861 tctcgctcat gctcctggct gagcactact tcttcgacct gctcgtcggc gtgctcgtcg
     1921 ccatcgccgt cgacctcgcg gtcgcccgcc tcgcgggacc ggtcgaggcc gtccccgccc
     1981 ccgcacgcgt cgactgacgt tcgctctcca cgcggcggcc cggcggcgag gccgagcaca
     2041 tctacacgac ggggaagtga cgaggggaga gcgcgcccgg cgagggtgag gcggacgagg
     2101 ccgggtcgta ccccgtttgg gcagtggata tcgggtgacg accacggcct ggtgcgcccc
     2161 accctgagcg gctcatgacg attgtagcga cgctcaccag ccctcgcccg tcgctgctcg
     2221 atctctgccg cacggccgct tgctaggatg acgccggccg gccctcccgg cggaaggaga
     2281 cattgatgac gcagcagacg ttcgtcctcg atcgcgatcg cgccgtggtc gtgatcatgg
     2341 acttccagca gggcatcgtg gggacggtcg cccgtgaccc cgagggcacc gttcgacgtg
     2401 ccgcctcggt cctcgccgcc gctcgcgccg cgtcggtgcc cgtcgtgcac gtggtgcacc
     2461 gcggcgggcg gttcgaggcg gagtcgccgg at
//
LOCUS       DAORPG010000271         2489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4698_length_2489_cov_2.892769, whole genome shotgun sequence.
ACCESSION   DAORPG010000271 DAORPG010000000
VERSION     DAORPG010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4698_length_2489_cov_2.892769"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="PLX85_06530"
     CDS             <1..1204
                     /locus_tag="PLX85_06530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HRC62865.1"
                     /translation="TLCGFAWNLPSLIVFRVLQGLGGGMLQPLGMAIVFTMITPLERP
                     RFMGLLGLPLLLAPILGPTVGGYLVEYSTWRAIFLINLPIGVVNVVLAWVLLKETPIR
                     GQAKMDFRGFALAAITFPCLLLGLSIGEESGWTSPDVLTIFVVGLGALAAFIAVELRQ
                     SDPMMQLRLFSHPMFRLAVGLQFVMQFSLFGLQFLLPIFLQAVRGLDAAHAGLVMFPM
                     GVVSFVTMTASGRLYNRVGPKPLVSIGLVVLAATTALLSQITEHTSVMYITALASGRG
                     LALGLCAMNVQTVAYNTVEQGQLPRATALTNVAFRVFGSIATALLTTTLVISLAFHGA
                     PAGSSITEGTAPIPFMVQAFRDAFLAMTALSIVGFALSFRLHDPVLEQERTRGRAPAR
                     ERELAAEL"
     gene            1398..>2489
                     /locus_tag="PLX85_06535"
     CDS             1398..>2489
                     /locus_tag="PLX85_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62866.1"
                     /translation="MGIWVGRYAIVGGEVREHGPWLVERDSYRDDEELRLLVLADPVD
                     ERSFEFAPEVAEAVAELFTRESLSITGGLLRALRQAHINLAEWNRRSLREHRVAVGLT
                     CVAVRGGDVTIAQVGPGAVYIAGPEGVRRLSTEGEDAARPLGGPEDIEPQFFSTHLGG
                     HTLLLLTGSVDQLIGTNAIGEALRVGAERTLAELYRRTRGVEDMTAAVIVEVADDVEA
                     ADEDERAPTNILDHRRGLEADAETADETEDVEETAAVHDGEGLTFAGSGAAAGEPASV
                     ATGEEPERPLLVTVPPEPETPEPVDSGVRREQEGQLGLRQRMPKLRRQTSSYPATAGR
                     GTGARPTSRGSSRVLGSDGSRMPSLGVPGN"
BASE COUNT          335 a          820 c          941 g          393 t
ORIGIN      
        1 gacgctctgc ggcttcgcat ggaacctgcc gagcctgatc gtgttccgcg tgctgcaggg
       61 cctgggcggc ggcatgctgc aaccgctggg catggcgatc gtgttcacga tgatcacgcc
      121 cctcgagcgg cctcggttca tggggctcct cgggctgccg ctgctgctgg cgcccatcct
      181 ggggcccacg gtgggtggct accttgtcga atactcgacg tggcgcgcga tcttcctcat
      241 caacctcccg attggcgtcg tgaacgtggt gctggcgtgg gtgctgctga aggaaacgcc
      301 gatccgcggc caggcgaaga tggacttccg cggcttcgcg ctggccgcga tcaccttccc
      361 ctgtctgctg ctggggctct cgattggcga agagtccggg tggacctcgc cggacgtgct
      421 gacgatcttc gtcgtgggcc tcggggcgct ggcagccttc atcgcggttg agctgcggca
      481 gtccgatccg atgatgcagc tgcgcctgtt cagccacccg atgttccgcc tggcggtcgg
      541 gctgcagttc gtgatgcagt tctcgttgtt cggcctgcag ttcctgttgc cgatcttcct
      601 gcaggcggtg cgagggctcg atgcggccca tgcgggcctc gtcatgttcc ccatgggtgt
      661 cgtgagcttc gtgacgatga cggcctccgg gcggctctac aaccgcgtgg ggccgaagcc
      721 gctcgtgagc atcgggctgg tggtgctggc cgcgacgacg gcgctgttga gccagatcac
      781 cgaacacacc tcggtcatgt acatcacggc gctggcgtcg ggccgcggcc tggcgctcgg
      841 gctgtgcgcg atgaacgtgc agacggtggc gtacaacacc gtggagcagg ggcagctgcc
      901 gcgggccacg gcgctgacca acgtggcgtt ccgggtgttc ggctcgatcg cgacggcgct
      961 gctgacgacg accctcgtga tcagcctggc gttccatggc gcgccggcgg gctcctcgat
     1021 caccgagggg acggcgccga tcccgttcat ggtgcaggcg ttccgcgacg ccttcctcgc
     1081 gatgaccgcg ctctccatcg tcggcttcgc actcagcttc cggctgcacg acccggtgct
     1141 ggagcaggag cggacgcgcg ggcgcgcgcc cgcgcgcgaa cgggagctgg cggcggagct
     1201 gtagacgagc ggggcggagc ctcgccgtcc gggatcgcag gctgtggcgt cgcccttttt
     1261 ccgttccggg gatgacctgc cccggcggcc cgcctatcat gtgccgcgca ccgagagcat
     1321 gagtcggccg tccgagtcgc gccccgacgg cgagacgaac ggcggcaccg cgttcgctcc
     1381 ccatcgaacg gccgggtatg ggcatctggg tcggacggta cgccatcgtc gggggcgagg
     1441 tgcgcgagca cggcccctgg ctcgtggagc gcgacagcta tcgcgacgac gaggagctgc
     1501 ggctcctcgt gctggccgac cccgtcgacg agcgttcgtt tgagttcgct ccggaggtgg
     1561 cggaggcggt cgccgagctg ttcacgcgcg agagcctctc aatcacgggc gggttgctgc
     1621 gggcgctgcg ccaggcacac atcaacctcg cggaatggaa ccgccgttcg ttgcgtgagc
     1681 accgcgtcgc cgtggggctg acgtgcgtcg ccgtgcgcgg aggcgacgtg accattgcgc
     1741 aggtcgggcc gggggcggtg tacatcgcgg ggcccgaagg cgtgcggcgg ctctcgaccg
     1801 agggtgaaga cgcggcgcgg cccctcggcg gtcccgagga catcgagccg cagttcttct
     1861 cgacgcacct cggcggacac acgctgctgc tgctgacggg cagcgtcgac cagctgatcg
     1921 ggacgaacgc gatcggggag gcgctgcgag tgggcgcgga gcgcacgctc gctgagctat
     1981 accgccgcac gcgcggcgtg gaggacatga cggccgccgt catcgtcgag gtggcggacg
     2041 acgtcgaggc ggcggacgag gacgaacgtg cgcccacaaa catcctcgac caccgccgcg
     2101 ggctggaggc agatgccgaa acggcagacg agaccgagga cgtcgaggaa accgcggcgg
     2161 tccacgacgg cgaagggctg accttcgccg ggtccggtgc ggcggccggc gagcccgcct
     2221 cggtggcaac gggcgaggag cccgagcgtc cgctgctcgt gacggtgccg cccgagccgg
     2281 agacgccgga gccggtggac tcgggcgtgc gccgggagca ggaaggccag ctcgggttgc
     2341 ggcagcggat gccgaagctg cgccggcaga cctcgagcta cccggccacc gccggacggg
     2401 gcacgggcgc gcgaccgacg tcgcgtggca gctcgcgagt gctgggcagc gacgggtcgc
     2461 gcatgccgtc gctaggggtt cccgggaac
//
LOCUS       DAORPG010000272         2484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4719_length_2484_cov_3.421161, whole genome shotgun sequence.
ACCESSION   DAORPG010000272 DAORPG010000000
VERSION     DAORPG010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4719_length_2484_cov_3.421161"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..786)
                     /locus_tag="PLX85_06540"
     CDS             complement(184..786)
                     /locus_tag="PLX85_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62867.1"
                     /translation="MALSIGGLLLTVGLAMQALTPAEHACWVARLGFDLDALYRFRLW
                     TMPFATVVQSDPGLGLDFWALLCSCVAAVALAERSAGWKFALAAFFITDWLASFLRLA
                     ALATLAGLGSSDARRFLDVADTGSSAALAGVLTVAALLHHGRTRALLLALLALWLGYG
                     VSRFRLDIGIVHAAGALAGVLLTLLWRRATDSSASASTAV"
     gene            862..>2484
                     /locus_tag="PLX85_06545"
     CDS             862..>2484
                     /locus_tag="PLX85_06545"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HRC62868.1"
                     /translation="MSRRVAAVVVALAALSNGVVAVALPVIVRFGARPNLLGTVLPFG
                     LHHWGRLLTPLFGLLLIYLAYHLWRRRRVAWWATLVAAGMAALAHLTKGDAIPLALGP
                     LLLAAMLLYARPVFTVHSDPWTVAQGLLLAAGIFVVAFAYGVAGFYYLDRRDFGITFS
                     TGDAVRRSVRQFALLGNADLHPRTRHARWFLDSLDVVTLASGALVALSIGLPLRNRYR
                     VWPRDREDVRALLERHGGTSLDYFKLWPGKTYFFTHERDAAIAYGVNSGCAVSLGDPV
                     GEPSQLEAAIEQFVALCRDNAWSPAFHQVPPRLLSTYRRCGLRVLKIGEEASVPLDAF
                     ASDTAHNKSFRNIQRRFEREGSTCTREEPPLSPGLIHELRTVSDSWLSLPGRRERGFT
                     LGRWDEQRLRDDVVYVVRSREGEALAFANLVPSYRAGEATIDLMRHRAQVMNGTMDFL
                     FLAIMTDLQARGFEWFSLGMAPFAGIGEEPDASLEERAIHELFERLNRFFSYRGLRAY
                     KSKFEPQWEGRYLAYAGGPLGLGRTTLALTRLT"
BASE COUNT          384 a          793 c          905 g          402 t
ORIGIN      
        1 acgcagtcgg cgcagaggtg gcagccgatg tcgcggaggg ggtggccgag ggcctgtcgt
       61 cgcctgagca cgcggcaagg acgagtgcga tggcgaggaa cgcaggcagg cgcaggcgga
      121 ggcgcatcgt gttctctcgt ttcggcgccg acgtgcgcgg attctacggc ctcgagggag
      181 cattcacacc gcggtcgacg ctgatgcgct cgaatcagtc gcgcgtcgcc agaggagggt
      241 gaggaggacg ccggccagcg ccccggctgc gtggacgatg ccgatatcga ggcgaaaacg
      301 cgagacgcca tagccgagcc acagggccag gagcgcgagg agcaacgccc tcgtgcggcc
      361 gtgatgcagg agcgcggcga ccgtcagcac gccggcgagt gcggccgacg aaccggtgtc
      421 ggcgacatcg aggaatctcc tcgcgtcgct cgaacccagg cccgcgagcg ttgcgagggc
      481 ggcgagcctg aggaacgacg ccagccagtc cgtgatgaag aacgcggcga gcgcgaactt
      541 ccatcccgcg ctgcgctcgg cgagtgcgac ggcggccacg cagctgcaca ggagcgccca
      601 gaagtcgagg ccgaggccgg ggtcggactg cacgaccgtg gcgaacggca tcgtccagag
      661 gcggaaccgg tacagcgcat cgagatcgaa gccgaggcgc gcgacccaac aggcgtgctc
      721 ggcgggggtt agcgcctgca tcgcgaggcc gacggtcaac agcaggcccc cgatgctcag
      781 ggccacaacc ggcaggcctc gtatcatcga agtactcgga gggtgagcgt gacgggcagc
      841 gtgcctcctc ggcgtcgggc catgagccga cgggtcgccg cggtggtggt ggccctcgcg
      901 gcgctctcaa acggggtggt ggcggtcgcc ctccctgtaa tcgtacggtt cggcgcacgc
      961 ccgaatctgc tcgggaccgt gctgcccttc ggcctgcacc actgggggcg gctcctcacg
     1021 ccgctctttg ggctgctcct gatctacctg gcgtatcacc tgtggcgtcg ccgtcgggtt
     1081 gcgtggtggg caacgctggt ggccgcgggg atggccgcgc tcgctcatct cacgaagggc
     1141 gacgctattc cactggcgct tgggcccctg ctgctggcag cgatgttgct gtacgcccgg
     1201 ccggtattca cagtgcacag cgacccatgg acagtcgctc agggcctcct gctggcagcc
     1261 gggatcttcg tcgtcgcgtt cgcgtatggc gtcgccgggt tctattacct cgaccggcgg
     1321 gacttcggca tcaccttttc gacgggggac gccgtccggc gcagcgtccg ccagttcgcg
     1381 ctcctcggaa acgcggacct gcatccgcgc acgcggcacg cgcgctggtt cctcgattcg
     1441 ctcgatgttg tgacgctggc cagcggggca ctggtggcgc tcagcatcgg gttgccgctg
     1501 cgcaaccggt accgggtgtg gccacgcgac cgtgaggacg tgcgagcgct cctcgagcga
     1561 cacggcggta cttcgctgga ctacttcaag ctctggccgg ggaagacgta tttcttcacg
     1621 cacgagcggg acgccgcgat cgcgtacggg gtgaattccg ggtgcgcggt ctcgcttggc
     1681 gaccccgtgg gtgagccctc gcagctggaa gccgccatcg agcaattcgt agcgctgtgc
     1741 cgcgacaacg cgtggtcccc ggcgttccac caggtgccgc ctcgtctgct gtcgacatac
     1801 cgccggtgcg ggctgcgtgt gttgaagatc ggcgaggagg cgagcgtgcc cctcgacgcg
     1861 ttcgcgagcg acaccgctca caacaagagc ttccgtaaca tccagcgtcg attcgaacgt
     1921 gaggggtcca cctgcacgcg agaggagccg ccgctgagcc cgggcctgat ccacgagctg
     1981 cggacggtct ccgacagctg gctttcactc ccagggcgtc gcgagcgagg cttcaccctc
     2041 ggccggtggg atgagcaacg tctgcgtgac gacgtcgtgt acgtggtgcg ctcgcgcgag
     2101 ggggaggcgc tggcgttcgc gaacctcgtg ccgtcgtacc gcgcggggga ggcgacgatc
     2161 gatctgatgc ggcaccgggc acaggtgatg aacgggacga tggacttcct gtttctcgcg
     2221 atcatgacgg atttgcaggc gcgaggattc gagtggttca gcctcggcat ggcgcccttc
     2281 gcggggatcg gggaggaacc ggacgcctcg ctggaggagc gggcgattca tgagctgttc
     2341 gagcgcctga atcgcttctt ctcttatcgg gggctgcgtg catacaagtc gaagttcgag
     2401 cctcagtggg aaggccgtta tctcgcctac gcggggggcc cgctcggcct cggacgaaca
     2461 acgctggcgc tgacgcgtct gacc
//
LOCUS       DAORPG010000273         2481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4730_length_2481_cov_3.497939, whole genome shotgun sequence.
ACCESSION   DAORPG010000273 DAORPG010000000
VERSION     DAORPG010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4730_length_2481_cov_3.497939"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..177)
                     /locus_tag="PLX85_06550"
     tRNA            complement(102..177)
                     /locus_tag="PLX85_06550"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(142..144),aa:Thr,seq:ggt)
     gene            complement(217..302)
                     /locus_tag="PLX85_06555"
     tRNA            complement(217..302)
                     /locus_tag="PLX85_06555"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(266..268),aa:Tyr,seq:gta)
     gene            complement(347..422)
                     /locus_tag="PLX85_06560"
     tRNA            complement(347..422)
                     /locus_tag="PLX85_06560"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(388..390),aa:Thr,seq:tgt)
     gene            568..1608
                     /locus_tag="PLX85_06565"
     CDS             568..1608
                     /locus_tag="PLX85_06565"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HRC62869.1"
                     /translation="MSAPPAPQPRRRPWLHLFIVGAVGVALAGAAIAFSGWWFFVGRY
                     RLPSIEADEWSPGAPPGTYRIELPSDGRDRYYLLHTPPRSAEPLPPLLVALHGGGSRP
                     EVMPALTGLLAVADREGFVVVLPAGIDGGWNDGRADSGQAAGRQGIDDIGFIRAVVED
                     ASKHALVDMARVFATGISNGAMMSSRLACEASDVFAGIAPVAGTAAEGFESWCRPARP
                     VAVMAFLGTADPLVPFEGGDVGGIFLGRGRGRVVSASDFEAFWSQHNRCERQPRDQLP
                     DADRSDGSRASVEAFVGCTGGGEMAVYVLDGGGHTWPGGRQYLSPRLVGHTNRDLSAS
                     ELMLQFFKGLRR"
     gene            1652..>2481
                     /locus_tag="PLX85_06570"
     CDS             1652..>2481
                     /locus_tag="PLX85_06570"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HRC62870.1"
                     /translation="MREVSRRGAMARFLQRSTTSQSVAAAASIVAFGFVASRLLGLLR
                     SVAIAHAFGTDPELSAYWVAFRLPDLVFQVLAGATLSAAFIPTYSRVRLRYGEQAGWD
                     LASAVLNLISIATTLAAVLAFLLAPLIVPLLAPGLGAETGREAELRSLAVELTRVMLL
                     SPILFGVSGMVTGILNARSHFLAPSLAPVFYNLAIIVAALFLAGPWGVKGLAAGVVVG
                     SALHLLVQLPALRMVGMRWRPTFDIVSAGVSEVARLMGPRVIGLAAAQLNFVVVVFFA
                     S"
BASE COUNT          311 a          849 c          853 g          468 t
ORIGIN      
        1 atcgtgccca cgttcgcgtg cggcttgttc cgctcaaact tccccttcgc catcgccttc
       61 tcctcggtat gcgtggtgct cgactgctga gatgtgaatg gtggagccct gaatgagatt
      121 cgaactcatg acctcgttct taccaagaac gcgctctgcc gactgagcta tcagggcccg
      181 tatgcctccg gtcgcgtcat gcgcggtgtt cattcgtggt gcagggggca ggattcgaac
      241 ctgcgtagcc gtaaggcggc ggttttacag accgctggat ttaaccactc tcccacccct
      301 gccgggatca tgctgctcgg cttccgacgc gcgcggcctc gcggtttgga gcccgagagg
      361 ggattcgaac ccgctaacct accgattaca aatcggttgc gctaccgttg cgccactcgg
      421 gcacgaactg cctcgcgtcg ccggacaaca aggatcgccc cccgaccggg gagcgagcaa
      481 ttcagtatac ggagtggccc gaacgacggt caacgcgagc gtggagcccg gtccggtctc
      541 gctccaccgg cgaagatgag tcagacgatg tcagcgccac ctgcacctca gccccgccgg
      601 cggccctggc tgcacctgtt catcgttggt gccgtagggg tggcgctcgc gggcgccgcc
      661 attgcgttct ccggctggtg gttcttcgtc gggcggtatc ggctgccctc gatcgaggcg
      721 gacgagtggt cgcccggcgc cccgccaggg acctatcgca ttgagctccc gtccgacggg
      781 cgcgaccgct actacctgct ccatacgcct ccgcgctccg cagagccgct gccgccgctc
      841 ctcgtcgcgt tgcacggcgg cggcagccgc cccgaggtga tgcccgcgct caccggcctg
      901 ctcgcggtcg ctgaccgcga aggattcgtc gtcgtgctgc cagcgggcat cgatggcggg
      961 tggaacgacg gacgcgcgga ctcgggccag gcggcgggcc ggcagggcat cgatgacatc
     1021 ggcttcatcc gcgcggtcgt cgaggatgcc tcgaaacatg cgctggtcga catggcccga
     1081 gtgttcgcca ccggcatctc caacggcgcc atgatgtcct cgcgcctcgc ctgcgaggcg
     1141 tccgatgtct ttgctggcat cgcgcccgtg gccggcaccg ccgccgaggg tttcgagtcc
     1201 tggtgccgcc cggcgcggcc ggtcgcagtg atggcgtttc tcgggactgc cgacccgctc
     1261 gtgcccttcg aaggcggcga cgtgggtggc atcttcctcg ggcggggccg cggccgcgtc
     1321 gtgagcgcga gcgacttcga ggcattctgg tcacagcaca accgttgtga gcgacagccc
     1381 cgcgatcagc tgccggacgc cgatcgctcc gatggctcgc gcgcgtcggt cgaggcgttc
     1441 gtcgggtgca ccggaggtgg ggagatggcc gtctatgtgc tcgacggtgg cgggcacaca
     1501 tggccgggcg ggcgccagta cctctcgcca cggctcgtcg ggcatacgaa ccgcgatctc
     1561 agtgcctccg agctgatgct gcagttcttc aagggcctgc gccgttagcg cggtttgacc
     1621 cgcgatccgc acggctcgat gatccgaggc gatgcgcgag gtttcccgtc gcggcgccat
     1681 ggcccggttc ctgcagcgca gtacgacctc gcagagcgtc gccgcggcag cgagcatcgt
     1741 cgccttcggg tttgtggcct cgaggctgct ggggctgctg cgctccgtgg cgatcgcgca
     1801 cgcctttggc accgaccctg agctgagcgc ctactgggtc gcgttccgtt tgccggacct
     1861 cgtcttccag gtgctggcgg gggccaccct ctccgccgcg ttcatcccga cctacagccg
     1921 ggtgcggctt cgctacggcg agcaggccgg gtgggacctc gcttcggcgg tgctgaacct
     1981 gatcagcatc gcgacgacgc tggcggccgt gctcgctttc ctgctcgcgc cgctcattgt
     2041 gccgctgctc gcacccggcc tcggtgcgga gacgggtcgc gaggcggagc tgcgctcgct
     2101 cgccgtcgag ctgacgcggg tcatgctgct ctcgccgatc ctgtttggcg tctccggcat
     2161 ggtcacgggc atcctcaacg cgcgcagcca tttcctcgca ccatcgctgg cgccggtgtt
     2221 ctacaacctc gcgatcatcg tcgccgcact ctttctcgcg gggccgtggg gcgtgaaggg
     2281 gctggctgcc ggcgtcgtgg tcggcagcgc gctgcacctg ctggtgcagc tgccggccct
     2341 gcgcatggtc ggcatgaggt ggaggccgac gttcgacatc gtgtcggcgg gcgtcagcga
     2401 ggtggcccgg ctgatggggc cgcgtgtgat cgggctcgcg gcggcgcagc tcaacttcgt
     2461 ggtggtcgtg ttcttcgcct c
//
LOCUS       DAORPG010000274         2478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4747_length_2478_cov_3.068510, whole genome shotgun sequence.
ACCESSION   DAORPG010000274 DAORPG010000000
VERSION     DAORPG010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4747_length_2478_cov_3.068510"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PLX85_06575"
     CDS             complement(<1..355)
                     /locus_tag="PLX85_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC62871.1"
                     /translation="MTDELPRNRRTARFERRVARVLGARAHDGSTLVVACSGGPDSTA
                     ALVASARARGASRELVAAYFDHGMRPPQETEMDRAAVRRIAVQVGTRVVCGATEGGAP
                     PASEAAAREARYEWLP"
     gene            complement(396..755)
                     /locus_tag="PLX85_06580"
     CDS             complement(396..755)
                     /locus_tag="PLX85_06580"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRC62872.1"
                     /translation="MRLPALRLPTFGPAHMVLAVAALLLGLFVYAAAQTATQTYRLHD
                     ERRTLAQQVEVLRKQKAELEGLREYLASDEYVESVARSQFGLVRPGETAVVVDSPAEP
                     APTRTPGERWWESLFSR"
     gene            1009..1371
                     /locus_tag="PLX85_06585"
     CDS             1009..1371
                     /locus_tag="PLX85_06585"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRC62873.1"
                     /translation="MCRAGSSTWTAQVDARRSAQVTKAELIAQVSKNAGISPKETETV
                     LNGFRDVVQASVKTGKDISYPGLGKFSRVSRKARTARNPRTGETLKVAATKVPRFVAS
                     EGFKRVVRGEAPAPKLAK"
     gene            1474..>2478
                     /locus_tag="PLX85_06590"
     CDS             1474..>2478
                     /locus_tag="PLX85_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018745267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRC62874.1"
                     /translation="MHDDLASAIDQIFPSVRAGLERLVRIPSVSAPDFDPAPVRTSAE
                     AVAGLLEGAGLEGVRLLEVEGAHPAVFGEVAAPAGAPTVLLYAHHDVQPPGDPADWSS
                     PPFEPVERNGRLYGRGASDDKAGLMVHVAALTALRGAMPVGVKVIIEGEEEIGSLHMA
                     DFLARYGDQLAADTIVIGDSANWRLGQPSLTTSLRGLIDCTVEVRTARHGVHSGMFGG
                     PLVDALTILTRLLATLHDNRGRPTVEGLVSRDAEPLDLTEEELREQAGVTAGLQFVGE
                     GSLTSRIWMQPAIAVLAIDAPPVAEAINQLVPVARAKVSVRLAPGDVPARAMAALEAH
                     L"
BASE COUNT          387 a          859 c          890 g          342 t
ORIGIN      
        1 gggggagcca ctcgtagcgc gcctcgcgag cggctgcctc ggacgctggt ggcgcccctc
       61 cctcggtggc cccgcataca acgcgggtgc cgacctgcac cgcgatgcgc cggacggccg
      121 cgcggtccat ctcggtctcc tgcggcggac gcatcccatg gtcgaaatac gcggcaacca
      181 actcgcgtga ggcgccgcga gcacgggcgc tggcgacgag tgcggcggtc gagtccgggc
      241 ccccggagca ggcgacaacc agcgtcgagc cgtcatgagc gcgcgccccg agcacgcggg
      301 cgacgcgacg ttcgaagcgc gcggtccgcc ggttgcgagg cagctcgtcc gtcatcgggg
      361 tcagggtagc gccagcggac gggaacgcgc ggagatcagc gcgagaagag cgactcccac
      421 cagcgctcgc cgggggtccg tgtcggagcg ggctcggcgg gggagtcgac gacgacagcc
      481 gtttcacctg gacgcacgag gccgaactgg ctgcgcgcga ccgactccac gtactcgtcg
      541 gaggcgaggt actcacgcag cccctcgagt tccgccttct gcttgcggag tacctcgacc
      601 tgctgcgcga gcgtccggcg ctcgtcgtgc aggcggtagg tctgcgtggc ggtctgggcg
      661 gcggcgtaga cgaagagtcc gaggagcagc gccgccacgg cgagcaccat gtgcgcgggg
      721 ccaaacgtgg gcaggcgaag cgcggggaga cgcatcgagt atgactgtcc ttatgtcccc
      781 gccggggatg accgtagcag acggggggag cggaagcaaa ctatccacgg atcgcacgac
      841 ctcgatcgtg ccggacagat ggccgcttgc cctcgcacat gccacaaacc ctgtgccgag
      901 gcggatttcc ggcatcgccg ggcctgaatc ccttatggga cgggaagaat tcggctgtgg
      961 atactttcca ataagccgcg cagcgcttta gcattagtcg cgacccgcat gtgccgggcg
     1021 ggctcctcga cgtggacggc gcaggtggac gcacgaagga gtgcacaggt gacgaaggcc
     1081 gagttgattg cgcaggtctc gaagaacgcg gggatctcgc ccaaggagac ggagaccgtc
     1141 ctcaacggct tccgcgatgt cgtgcaggcg tccgtgaaga cgggcaagga catctcgtac
     1201 ccgggcctcg ggaagttcag ccgcgtctcg cgcaaggcgc gcaccgcccg caacccccgc
     1261 accggtgaga cgctgaaggt cgccgcgacg aaggtgccgc gcttcgtggc cagcgagggg
     1321 ttcaagcgcg tcgtgcgcgg agaggccccg gcgccgaagc tcgccaagta ggcacttgca
     1381 tcgggagccc gtcactgagg gccgccgccg ggcggccctc ggcgcgcccg acgcctcacg
     1441 ctccacgccc ctccgccctg ataggctgct cccatgcacg acgacctcgc ctccgccatc
     1501 gaccagatct ttccgagcgt gcgcgcgggc ctcgaacggc tcgtccggat tccgtccgtc
     1561 agcgcacccg acttcgatcc ggcccctgtc cggacgtcgg cggaggcggt ggcggggctg
     1621 ctcgaagggg cgggactcga gggagtgcgg ctgctcgagg tcgagggggc gcatccggcc
     1681 gtcttcggcg aggtcgctgc gccagcaggc gcgcccaccg tgctgctcta cgcgcaccac
     1741 gacgtgcagc cgcccggcga ccccgccgac tggagctcgc ccccgttcga accagtcgag
     1801 cgcaacggcc ggctgtacgg acgcggcgcg tccgatgaca aagcaggcct catggtgcac
     1861 gtggccgcgc tgacggctct gcgtggggcg atgccggtcg gggtgaaggt catcatcgag
     1921 ggcgaggagg agatcggttc gctgcacatg gccgatttcc tggcccggta cggcgaccag
     1981 ctggccgcgg acaccatcgt gatcggcgac tcggcgaact ggcggctcgg gcagccgtcg
     2041 ctgacgacct cgttgcgcgg actgatcgac tgcaccgttg aagtgcgcac cgcccgccac
     2101 ggcgtgcaca gcggcatgtt cggcggaccg ctcgtcgatg cgctcaccat cctcacgagg
     2161 ctactcgcga cgctgcacga caaccgaggc cggcccaccg tcgaagggct cgtctcgcgc
     2221 gacgcggagc cgctcgacct caccgaggag gaactgcgcg aacaggccgg cgtcacggcg
     2281 ggtctgcagt ttgtcggcga gggctcgctc acctcgcgga tctggatgca gcccgccatc
     2341 gcggtgcttg ccatcgacgc gccgccggtc gccgaggcga ttaaccagct cgtgccggtc
     2401 gcacgcgcca aagtcagcgt ccggctcgcg cctggcgacg tccccgcgcg cgcgatggcg
     2461 gccctcgaag cgcacctg
//
LOCUS       DAORPG010000275         2476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4757_length_2476_cov_3.116481, whole genome shotgun sequence.
ACCESSION   DAORPG010000275 DAORPG010000000
VERSION     DAORPG010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4757_length_2476_cov_3.116481"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /locus_tag="PLX85_06595"
     CDS             <1..1441
                     /locus_tag="PLX85_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRC62875.1"
                     /translation="VSVFLMLGVGLVKGLTGQLGDVQDVHGTIEATQSMTLLLLLRAY
                     ASGNTAVTGVEAISNGIPAFADPKPKNAAITMAIMGSLVAVMFLGITQLAHVTHAQAS
                     ESETVISQLGRTVFSAHSPFYVAVIFGTAAILILAANTSFVDFPRLAALQAGDRFLPG
                     WLMDRDNRLVFGVGISVLSVAAGALLIVFQASVTHLIPLYAVGVFLSITLSQAGMAVH
                     WRRSGRLRPGEQLPRYSPDGELVTTIEHDGGWHWKLALSAAGATMTAVITCIFAVAKF
                     TQGAWIIVIVIPTLLVVFFRIHRHYRTVQRAMTLQRDEIRPYLEQPVVTLHLLVVAGL
                     QRHTLPALREFVHMPGYGGMRQAVHVDVNEQATAELRQQWRELGIDAMGLPLVILPSH
                     YGAGDVIDTLVSYVRGALSVDDSLRVEVVITDWASNTSWWGWLLTPALHHLTGARLRL
                     AFLAEDRATVKNYRTLTSRPGLPAPSHRS"
     gene            1502..2239
                     /locus_tag="PLX85_06600"
     CDS             1502..2239
                     /locus_tag="PLX85_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRC62876.1"
                     /translation="MARPSLRLLHCADLHLTHSAWDASIATLRALREVATQRGAEQVL
                     MAGDLFDSSKQPDAFSDAVAEEMAQFPVFVAAIPGNHDLLYSRSDPDPFGRLFDQLGR
                     EALAVTDEAGACVELAEGRMRLWGRGMPEHTPENDPLAGLPLLDEGGSWRVAMAHGYL
                     MDAPNGRSSPIIPGRHRAALGTYDYVALGHHHSPHRLDFEGTLLMDSGSASPIVGHGS
                     YAIVDLEESGVRVELGELPTSQYRRMR"
     gene            complement(2255..>2476)
                     /locus_tag="PLX85_06605"
     CDS             complement(2255..>2476)
                     /locus_tag="PLX85_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62877.1"
                     /translation="DLELRGPGDTFGTRQSGLPSLHVASLLDAQLIDATRREAEQVLD
                     SDPRLEAPEHQGLRRLAARVAENVVAEVH"
BASE COUNT          368 a          875 c          835 g          398 t
ORIGIN      
        1 cgtctcggtg ttcctgatgc tgggcgtcgg cctcgtgaag gggctcacgg ggcagctggg
       61 cgacgtgcag gacgtgcatg gcaccatcga ggcgacccag tcgatgaccc tcctgctcct
      121 gctgcgcgcc tacgccagcg gaaataccgc cgtgacgggc gtcgaggcga tcagcaacgg
      181 catcccggca ttcgcggatc ccaagccgaa gaacgccgcc atcacgatgg cgatcatggg
      241 ctcgctggtg gccgtcatgt tcctcggcat cacccagctc gcccacgtga cgcatgcgca
      301 ggcgagcgaa agcgagactg tgatctcgca gctgggccgg acggtcttca gcgcgcactc
      361 accgttctac gttgcagtca tcttcgggac ggccgcgatc ctgatcctgg ccgcgaacac
      421 ctcgttcgtg gacttccctc gcctggcggc gctgcaggcc ggcgaccgct tcctgcccgg
      481 ctggctcatg gaccgggaca accgcctcgt gttcggcgtc ggcatctccg tgctgtccgt
      541 tgcggcgggc gcgctgctca tcgtgttcca ggccagcgtg acccatctca tcccgctgta
      601 cgccgtcggg gtgttccttt cgatcaccct cagccaggcc ggcatggccg tgcactggcg
      661 acgcagcggg cggctgcggc cgggtgagca actgccgcga tactcgcccg atggcgagct
      721 cgtcacgacg atcgagcacg acggcggctg gcactggaaa ctcgcgctga gcgccgcggg
      781 cgcaacgatg accgccgtca ttacctgcat cttcgccgtc gcgaagttca cgcagggcgc
      841 gtggatcatc gtcatcgtga tccccacgct cctcgtcgtg ttcttccgca tccatcgtca
      901 ttaccgcacc gtccagcgcg cgatgacgct gcagcgagac gaaatccgcc cgtacctcga
      961 gcagccggtg gtgacgctgc acctgctggt ggtcgcgggg ctgcaacggc acacgctccc
     1021 ggcgctccgc gagtttgtgc acatgccggg ctacggaggg atgcggcagg cggtgcacgt
     1081 ggatgtcaac gagcaggcga cggccgagct gcggcagcag tggcgtgagc tggggatcga
     1141 cgccatgggg ttgcccctcg tgatcctgcc gagccactac ggcgccgggg acgtcatcga
     1201 cacgctcgtc tcgtatgtgc ggggagccct gtccgtggac gactcgttgc gcgtcgaggt
     1261 ggtgattacg gactgggcct cgaacacgag ctggtggggt tggctcctga caccggcact
     1321 gcatcacctc accggcgcac gcttgcgcct cgccttcctc gccgaggacc gcgcgaccgt
     1381 gaagaactac cgcaccctga cctcacggcc gggactccca gcgccgtcac accgctcgta
     1441 gcggcgcacg ggcgccacca ggcgcactgg cgggcggcga tagagcggga cacactgtgg
     1501 catggctcga ccctcgctgc ggctgcttca ctgcgccgac ctgcacctca cccactccgc
     1561 gtgggacgcc tcaatcgcga cgctgcgcgc gcttcgcgag gtggccacac aacgaggcgc
     1621 cgagcaggtg ctcatggctg gcgacctctt cgactcctcg aagcagcctg acgcgttcag
     1681 cgacgcggtc gccgaggaga tggcgcagtt cccggtgttc gtcgccgcga tccccgggaa
     1741 ccacgacctt ctctactcgc ggtcggaccc cgaccccttc gggcggctgt tcgaccaact
     1801 cggccgcgag gcgctggcgg tgaccgacga ggccggagca tgcgtcgagc tggccgaagg
     1861 gcggatgcgc ctctggggac gtggcatgcc agagcacacg cccgagaacg accccctcgc
     1921 ggggctgccg ctcctcgatg agggcggctc gtggcgggtc gcgatggcgc atggctacct
     1981 gatggacgcg cccaacgggc ggagctcgcc gatcatcccc gggcgccacc gggccgccct
     2041 cggcacgtac gactacgtcg ccctcggaca ccaccactcg ccgcatcgcc tcgatttcga
     2101 gggcacgctg ctgatggaca gtggttccgc atcgccgatc gtcggtcacg ggtcgtacgc
     2161 gatcgtcgac ctcgaggaga gtggcgtccg ggtcgagctg ggggagctgc ccacgagcca
     2221 gtaccgccgc atgcgctgac gtccgaacct cggctcaatg cacctcggca accacgtttt
     2281 cggcgacgcg cgcggcaagc cggcgcaggc cctggtgctc gggcgcctcg agccgcgggt
     2341 cgctatcgag tacctgctcg gcctcgcggc gggtcgcgtc gatgagctgc gcgtccagca
     2401 acgaagcgac gtgcagcgac ggcagccccg attgacgcgt cccaaacgtg tcgccggggc
     2461 cgcgcagctc gaggtc
//
LOCUS       DAORPG010000276         2472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4775_length_2472_cov_3.331403, whole genome shotgun sequence.
ACCESSION   DAORPG010000276 DAORPG010000000
VERSION     DAORPG010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4775_length_2472_cov_3.331403"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PLX85_06610"
     CDS             <1..929
                     /locus_tag="PLX85_06610"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HRC62878.1"
                     /translation="NEPANLMTPTIVAERAQEVAAATGLECTIIERAEAERLGMGSYL
                     SVANGSVQPPKFIVLRYRNGGRTRPVGLIGKGITFDTGGISIKPAAGMERMKGDMTGA
                     ASVIGAMQAIARLGPAVNVLAIAPCTENMPSGSATKPGDVVYAMDGQSIEVLNTDAEG
                     RLVLADGLAYARRENCSTLVDVATLTGAMNVALGNVRIGTFANNERLYADLERAAAAA
                     GERLWRMPLDAEYFDLIKSDVADIKNTGGPSAGSITAAKFLERFAHDTPWAHLDIAAV
                     SDSTAEKGVLVKGSTGSPVRTLVHFVLGRART"
     gene            1018..2082
                     /locus_tag="PLX85_06615"
     CDS             1018..2082
                     /locus_tag="PLX85_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRC62879.1"
                     /translation="MRIAQVAPLFERVPPRLYGGTERIVAYLTDELLALGHDVTLFAS
                     GDSITEARLVPVGKQALRLDGTVVQETVPHILMLEQVAQRAREFDVVHFHTDHLHYPL
                     ARRLRTPHVTTLHGRLDHAELQSLYREFSDIPVISVSKEQQQPLPHAGWSGAVLHGLP
                     ADLYRQGDGRGGYLAFLGRLSPEKRPDRAIEIARRTGIPLRIAAKVGADDRRYFHDVI
                     EPLLADPLVEFIGEIGEAEKQDFLGNARALLFPIDWSEPFGMVMIEAMACGTPVVAWR
                     NGAVPEVIESGVSGFIVDNLDDAVRATAAACALDRTRARAAFEERFTARRMALDYLRI
                     YREVIAGRTGPAAEAAPLAS"
     gene            2123..>2472
                     /locus_tag="PLX85_06620"
     CDS             2123..>2472
                     /locus_tag="PLX85_06620"
                     /inference="COORDINATES: protein motif:HMM:NF026092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HRC62880.1"
                     /translation="MTSSDEHLIHIRSTASDDRSRVLKHGDTFAVCNRFGDIQPVETG
                     DRGLYHDGTRHLSHLVLRISGVRPLLLSSGITSDNATLMVDMTNPELPGDEGGLCPQD
                     TLHLLRSKLIYGAA"
BASE COUNT          391 a          958 c          741 g          382 t
ORIGIN      
        1 ccaatgagcc ggcgaacctg atgaccccca cgatcgtcgc cgagcgcgcg caggaggtcg
       61 ccgccgcaac cggcctcgaa tgcaccatca tcgagcgcgc cgaggccgaa cgcctgggta
      121 tgggctcgta cctcagcgtc gcgaacggtt ccgtgcagcc cccaaagttc atcgtgctgc
      181 gctatcgcaa tggcggccgc acgcggcccg ttggcctcat cggcaagggg atcacctttg
      241 ataccggcgg catctccatc aagccagccg ccggcatgga gcgcatgaag ggcgacatga
      301 cgggcgccgc ttcagtcatc ggcgcgatgc aggctatcgc gcgcctcggc ccggccgtca
      361 acgtcctcgc catcgctccc tgcaccgaga acatgccgag cggctctgcc accaagccgg
      421 gcgacgtcgt ctacgcgatg gacggccagt ccatcgaagt cctcaatacc gacgccgagg
      481 gccgcctcgt cctcgctgat ggccttgcat acgcccgccg cgagaattgc tcgacgctgg
      541 tcgacgtcgc cacgcttacc ggcgcgatga acgtcgccct cggcaacgtc cgcatcggca
      601 ccttcgcgaa caacgagcgc ctctacgccg acctcgagcg cgccgccgcc gccgccggcg
      661 agcgcctctg gcgcatgccc ctcgatgccg agtatttcga tctcatcaag tcggacgtcg
      721 cggacatcaa gaacacaggc ggcccctccg ccggctcgat caccgccgcg aagttccttg
      781 agcgcttcgc ccacgacacc ccgtgggcgc atctcgacat cgccgctgtc tccgattcga
      841 ctgccgaaaa aggcgtgctc gtcaaaggca gcacgggctc gcccgtgcgc acgctggtgc
      901 acttcgtcct cggccgcgcg cggacgtagc gtccgaggaa ttccgccgct ccggaatccc
      961 ttgtccccct tgctacattg gtcaacgggg cgacaggagg cagtaaggag cggcggagtg
     1021 aggatcgcgc aagtcgcgcc gttgtttgaa cgcgtcccgc ctcgcctgta cggcgggacc
     1081 gagcggatcg tcgcgtacct caccgacgag ctcctggccc tgggccacga cgtcacgctc
     1141 ttcgccagtg gcgactcgat cacggaggcg cgcctcgtcc ccgtgggcaa gcaggcgctg
     1201 cgcctcgatg gcaccgtcgt gcaggagacc gtcccgcaca tcctgatgct cgagcaggtc
     1261 gcgcagcgcg cccgtgaatt cgacgtcgtg cacttccaca cggaccacct gcactacccg
     1321 ctggcccgcc ggctgcggac tccgcacgtg accacgctcc acggtcgcct cgaccacgcc
     1381 gagctgcagt cgctgtaccg cgagttctcc gacatccccg tcatctccgt ctcgaaggag
     1441 cagcagcagc cccttcccca tgccggctgg tccggcgccg tgctccacgg tctgcccgcg
     1501 gacctctacc ggcagggtga tggacgcggc ggctacctcg cattcctcgg gcggctctcg
     1561 ccggagaagc ggccagaccg cgcgatcgag atcgcgcgcc gcaccggcat ccctctgcgc
     1621 atcgccgcca aagtcggggc agacgaccgc cggtactttc acgacgtgat cgagcccctg
     1681 ctcgctgacc cgctggtcga gttcatcggc gagatcggcg aggccgagaa gcaggacttc
     1741 ctcggcaacg cccgcgccct gctcttcccc atcgactggt ccgagccctt tggcatggtc
     1801 atgatcgagg ccatggcgtg cggcacccca gtcgtcgcct ggcgcaacgg cgcggtcccc
     1861 gaggtgatcg agtccggcgt ctcgggcttc atcgtcgaca acctcgatga cgccgtgcgc
     1921 gcgaccgccg ccgcctgcgc cctcgaccga acccgcgccc gcgccgcctt cgaggaacgg
     1981 tttacggcgc gccgcatggc gctcgattac cttcgtatct atcgtgaggt gatcgccgga
     2041 cgcaccggac cggccgccga ggctgccccc ctcgcgtcct gacggcacct cgccgctccc
     2101 cgcgctcgac ctcggacgcc gcatgaccag ctccgacgaa cacctgatcc atatccgctc
     2161 cacggcatcg gacgaccgca gccgcgtcct caaacacggc gacacgtttg ccgtctgcaa
     2221 ccgctttggc gatatccagc cggtcgagac cggcgaccgc ggcctctacc acgacggcac
     2281 ccgccacctc tcacacctcg tgctgcggat cagcggcgtg cgcccgctgc tcctcagctc
     2341 cggcatcacc tcggacaacg ccacgctcat ggtcgacatg acgaaccccg agctacccgg
     2401 cgacgagggc ggcctctgcc ctcaggacac gctccacctg ctgcgctcca agctcatcta
     2461 cggcgccgcc tg
//
LOCUS       DAORPG010000277         2455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4838_length_2455_cov_3.167917, whole genome shotgun sequence.
ACCESSION   DAORPG010000277 DAORPG010000000
VERSION     DAORPG010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4838_length_2455_cov_3.167917"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLX85_06625"
     CDS             complement(<1..184)
                     /locus_tag="PLX85_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62881.1"
                     /translation="MRTGTSDPLAGVWGSRVADQRRRGHQRLGGVEVDRDEAKRRFER
                     IDQRDRGAVEVDEVRRP"
     gene            198..1685
                     /locus_tag="PLX85_06630"
     CDS             198..1685
                     /locus_tag="PLX85_06630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC62882.1"
                     /translation="MLRILAGTIRIDLPEGGLATIGAQLDSAVVLDGSGQVVVDRGTV
                     RKPTPASPAFLEAVAAASESARTDSPGPDGVGTWIAGAAPVGNSAWRAVTLLDQQAAF
                     ADSRRALLLELGALLLVALLGALLTIGTARRLNRRTTLERAEAEQVRRREAFFHELAD
                     ALPVVGGTLDTGLSTTFANRTLRAARTSQLLELLHPEDAETLRTAEGDADADSTFGLD
                     IRLATGPRGADYRWHRLNLVPAPQLPGARWFFAATDIEDEKRAELGLQRDIQQRDEFL
                     GLVSHELRNPLAAIIGNASMLTGRYAAELPKDAYESAEEIDLSARRLRRLVENMLVLS
                     QAGNPESPPPIEPQLVPRLLGATQADFHARYPAHSLRLHLDPDLPVVLANETFVDQIL
                     WNLLTNAAKYGGETRIIDLTARRAGDAVEVSVADRGPGLAPDELDRVFDAHFRSRTAR
                     AQAEGLGLGLSVCRRLVEAQGGVIEARPTPGGGTTVSFTLPIAED"
     gene            1752..1940
                     /locus_tag="PLX85_06635"
     CDS             1752..1940
                     /locus_tag="PLX85_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62883.1"
                     /translation="MALERCEFCTARPREEVAVLRWRGDDRDRLTLWLCGKHLERMQK
                     AGTRGWEHSGWLHKVGWW"
BASE COUNT          364 a          969 c          756 g          366 t
ORIGIN      
        1 cgggccgtcg cacttcgtcg acttcgacgg cgccgcggtc acgctggtcg atgcgttcga
       61 aacgccgttt ggcctcgtcc cggtcgacct cgacaccgcc gaggcgctga tggccgcgac
      121 gccgctgatc cgcaacgcgg ctgccccaca cgcccgcgag cgggtcgctc gtgccggttc
      181 gcaacgatcg cggcgaggtg ctgcgcatcc tcgccggcac gatccgcatc gacctccccg
      241 agggcggcct cgccaccatc ggcgcgcaac tggacagcgc ggtcgtcctc gacggcagcg
      301 gccaggtggt cgtcgaccgt ggcacggtcc gtaagcccac tcctgccagt cccgcgtttc
      361 tcgaggccgt cgccgcggcc tccgagtctg cccgcacgga cagccccgga ccggacggcg
      421 tcggcacctg gatcgccggc gctgccccgg tgggcaactc cgcctggcgc gccgtgacgc
      481 tcctcgatca gcaggccgcg ttcgctgact cgaggcgcgc actcctcctc gagttgggtg
      541 cgctcctgct ggttgcattg ctcggcgcct tgctcaccat cggcacggcg cgcaggctca
      601 accgccggac gaccctcgaa cgcgccgagg cggaacaggt ccgccgccgc gaggcgttct
      661 ttcacgagct cgccgacgcc ctgcccgtcg ttggcggcac cctcgacacc ggcctcagca
      721 ccacgttcgc caaccgtacc ctgcgcgcgg cccgcacctc gcaactcctc gagctgctcc
      781 atccggagga cgctgagacg ctccgcaccg ccgaaggcga cgccgatgcc gacagcacgt
      841 tcggcctcga cattcgcctc gcgaccggcc cccgaggcgc cgactaccgc tggcatcgcc
      901 tcaacctcgt cccggcgccc cagctgccgg gcgcgcgctg gttcttcgcc gccaccgaca
      961 tcgaggacga aaagcgcgcg gagctcggac tccagcgcga tatccagcag cgcgacgagt
     1021 tcctcggatt ggtctcccac gagctacgca accccctggc cgccatcatc ggcaacgcct
     1081 cgatgctcac cgggcgctac gccgccgagc tgcccaagga cgcctacgag tccgccgaag
     1141 agatcgatct cagtgcccgc cggctccgcc ggctcgtcga gaacatgctc gtgctctcgc
     1201 aggccgggaa ccccgagagt cccccaccca tcgaaccgca actcgtcccg cgtctcctcg
     1261 gcgccaccca ggccgacttc cacgcccgtt acccggcgca cagtctgcgg ctgcatctcg
     1321 atccagacct cccggtcgtg ctcgccaacg agacgttcgt cgaccagatc ctctggaacc
     1381 tcctgacgaa cgccgccaag tatggcggcg aaacgaggat catcgacctc accgcccggc
     1441 gtgccggcga cgcggtcgag gtcagcgtcg ccgaccgcgg acctggcctc gccccagacg
     1501 aactcgaccg cgtcttcgac gcgcattttc gctcccgcac cgcgcgcgcc caggccgagg
     1561 gcctcgggct cggcctctcc gtctgccgtc ggctggtcga agctcagggc ggcgtgatcg
     1621 aggcacgccc cacccccggc ggtggcacca ccgtctcctt cacgctcccc atcgccgagg
     1681 actgagcggc acgcgccctc ccccgctgct acgatcgccg cgcaccccct cgatctgaag
     1741 gaacaaggtc aatggccctc gaacgctgcg agttctgcac cgcgcgcccc cgcgaggagg
     1801 tcgccgtcct ccgctggcgc ggcgacgacc gcgaccgcct gaccctctgg ctctgcggca
     1861 agcacctcga gcgcatgcag aaggccggca cgcgcggctg ggagcactcc ggctggctcc
     1921 acaaagtcgg ctggtggtag cggaggacgg acgctggtaa cgcggctcga ggtcggcgaa
     1981 gatccaccag ccagacgccg tgtcgccgcc tgacgcggcc cccctccacc tgcgcccctc
     2041 ggtcgaactc gcgctgggtc gaactgccga agctcgcatc gagagcctcg atctctgcat
     2101 gtgggaactc cgaccgccct acctgcgtcg gcagatatgc gctagattca gttggaatag
     2161 cggcgtctca gctagaaagg tagtcgacgt cgctctggcc tgacccaggc cgactcggac
     2221 tgcttctgaa cccactgaca gttcctcgcg cctcgcagtc gaagggcttt cccacatgtt
     2281 gcctcggcta ttgccggtgc gcttccgatc ccaactcatc ggcgtggccg ccggtgcagc
     2341 agtgacggcc gccctcctgg ggggcgtcgc cgtcgtcagc gcccatggcg gtgacacgaa
     2401 ctccatccat gcctgcgtga acaagacgaa cggccaggta cgcgtggtcg gcgcg
//
LOCUS       DAORPG010000278         2449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4870_length_2449_cov_3.231412, whole genome shotgun sequence.
ACCESSION   DAORPG010000278 DAORPG010000000
VERSION     DAORPG010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4870_length_2449_cov_3.231412"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(246..1865)
                     /gene="cimA"
                     /locus_tag="PLX85_06640"
     CDS             complement(246..1865)
                     /gene="cimA"
                     /locus_tag="PLX85_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HRC62884.1"
                     /translation="MTPTNVLLYDTTLRDGLGMEGLSLSLEEKLLIVQKLDDLGVHYI
                     EGGYPGSNPKDAEFFQRARELRLRHARLVAFGSTRRAGGDTETDSALLAVLNAGTPVV
                     TLVGKSSAAQVREVLETSEEENLAMIGDSVRFLRERGREVVFDAEHFFDGFAADPEYA
                     LATLRAAANAGASTVTLCDTNGGAMPDAIVRGVRAAAEAVACAVGIHCHNDAELAVAN
                     TLLGVQAGATQVQACLNGWGERCGNANILTVIANLKLKLGIDVVSDEQLARLTEVSNF
                     ASELANLAPNPQQPYVGSSAFAHKAGLHVAAITKSAGSYTHVDPLAVGNTERILVSEL
                     AGRRNVLEKLREQGVGIELTDAQAREVLERVKAQEARGYQFESAEASFELLVRRSLDG
                     YVAPFGLEDFLIVERRRHLDANAAVAPDGPASDSEMQAEAMVKVRVGERLTQTAADGN
                     GPVSALDAAVRRALSESYPALEKVHLTDYKVRIINSGGGADAAVRVLIESGDGLHIWR
                     TVGASTDVIEASWLALQDAYEYWIRHWGHVS"
     gene            2175..>2449
                     /locus_tag="PLX85_06645"
     CDS             2175..>2449
                     /locus_tag="PLX85_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62885.1"
                     /translation="MLDYHREPRAGECDVCDDSLQHQLDLPYEHARVRLSTVFLTQDL
                     EIEPETTKLSSLAVSRSGLEPGPVHDLDPRHWFWAICDEPGIRLSWE"
BASE COUNT          362 a          790 c          890 g          407 t
ORIGIN      
        1 cctcagcgtc gctgccgcag ccgagggcga gcagggcagc ggcggtcgtc gggacactgc
       61 cggtaacgag caggacgcgc ttcacgaggt cgcgccgccc cagcttccgc tcgtggtact
      121 cctccgcgaa ctcctcgatg aggtatcgct ggtggatgtt cagttccatg gcaggcgctc
      181 caatctccgc cgtggccggc tgagagaggg tacgttcgag gtgcgcggcg ggatccggcg
      241 gagtgtcagc tcacgtggcc ccagtgccga atccagtact cgtatgcgtc ctgcagcgcg
      301 agccacgatg cctcgatgac gtcggtcgag gcgccgacag tgcgccagat gtggagcccg
      361 tcgcccgact cgatgaggac gcgcacggcg gcgtcggcgc cgccacccga gttgatgatg
      421 cgcaccttgt agtccgtgag gtgcaccttc tcgagcgccg ggtaggactc gctgagcgcg
      481 cgccgcacgg ccgcgtcgag ggcggacacg gggccgttgc cgtcggcggc cgtctgcgtc
      541 agccgctcgc cgacgcgcac cttcaccatc gcttccgcct gcatctcgct gtcgctggcc
      601 gggccgtcgg gagccacagc ggcgtttgcg tcgaggtggc gacggcgttc gacgatcagg
      661 aaatcctcga ggccgaaggg cgccacatag ccatcgaggg agcggcgcac gagcaattcg
      721 aaggacgcct cggcggactc gaactgatag ccgcgcgctt cctgcgcctt cactcgctcg
      781 agcacctcgc gggcctgggc gtcggtcagc tcgatgccga cgccctgctc gcgcagcttc
      841 tcgaggacgt tgcggcggcc ggcgagctcc gagacgagga tgcgctccgt gttgccgacg
      901 gcgagcgggt cgacgtgggt gtaggaaccg gcgctcttcg tgatcgccgc gacgtgcagg
      961 ccggccttgt gtgcgaaggc cgaggatccc acgtacggct gctgtgggtt cggcgccagg
     1021 ttcgcgagct ccgaggcgaa gttcgagacc tcggtcagcc gcgcgagttg ctcgtcgctc
     1081 acgacgtcga tgccgagctt gagcttgagg ttggcgatca cggtcaggat attggcgttg
     1141 ccacagcgct caccccagcc gttgaggcag gcctgcacct gagtcgcccc tgcctgcacg
     1201 ccgaggaggg tgttcgcgac ggccagctcg gcgtcgttgt ggcagtgaat gccgacggcg
     1261 cacgcgaccg cctcggcggc ggcgcggacg ccccgcacga tggcgtccgg cattgcgccg
     1321 ccgttggtgt cgcagagcgt cacggtgctg gcgccggcat tggcggcggc gcgcagcgtc
     1381 gcgagggcgt actcggggtc ggcggcgaag ccatcgaaga agtgctcggc atcgaagacc
     1441 acctcgcggc cgcgctcgcg caggaagcgc accgagtcgc cgatcatcgc gaggttctcc
     1501 tcctcggacg tctcaaggac ctcgcggacc tgagcggcgg aggacttgcc gacgagcgta
     1561 acgacgggcg tcccggcgtt caggacggcg aggagcgcgg agtccgtctc ggtgtcgccg
     1621 cccgcacgcc tcgtggagcc gaacgcgacg aggcgcgcgt gccgaaggcg cagctcacgg
     1681 gcacgctgga agaactcggc gtccttgggg ttggagcccg ggtagccgcc ctcgatgtag
     1741 tgcacgccga ggtcgtccag cttctggacg atgagcagct tctcctcgag ggaaagcgag
     1801 aggccttcca tgccgaggcc gtcgcggagg gtggtgtcgt agaggaggac gttggtcggg
     1861 gtcatcggtg actcgcgctt ctcatgcggg ccgcgtccgg atgggattcg aggtgcctcg
     1921 aagcgggctc ctcgcggggc cgttggcgtg acctgcggga tggctggtgg tgcgtgctag
     1981 cgcgcgatga tcgaagccgc cggccctgag ggccggtcga ccgtcaccac ctgtcgtgag
     2041 cgctgagcag gagtcataac ccgtcgattg tagggacggg gacgggtgac tcgcaacggc
     2101 ggaagaactg atcacttttc tgcgcgcaca tttggtctgg tacagtgcct ggtgggcagg
     2161 acagggtggc gaggatgctc gactaccatc gtgagccgcg ggcgggtgag tgcgatgtct
     2221 gtgacgactc gctccagcac cagctcgacc ttccttacga acatgcccga gtacggctct
     2281 ccaccgtctt cctgacgcag gatctcgaga tcgagcctga gacgacgaag ctcagttccc
     2341 tcgctgtctc ccggtccggt ctcgagcccg gaccggtgca cgacctcgat ccgcggcact
     2401 ggttctgggc catctgcgac gagccaggca tccgcctgtc ctgggagag
//
LOCUS       DAORPG010000279         2441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4898_length_2441_cov_3.826488, whole genome shotgun sequence.
ACCESSION   DAORPG010000279 DAORPG010000000
VERSION     DAORPG010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4898_length_2441_cov_3.826488"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..697)
                     /locus_tag="PLX85_06650"
     CDS             complement(149..697)
                     /locus_tag="PLX85_06650"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC62886.1"
                     /translation="MSQHVIILSGPPGAGKTAVAEALCERYDRMLHVEVDELRHMVKA
                     GYRHPWTGDAQAHEQLVLAARNASAIARESVAMRYAVVIDDVVPAEMAKVYHEALAGA
                     GVPVHLVTLLPTIEVTLARDASRRFSIPERVHVLHERLASEAESGALPGTVLDSSGDR
                     DAALTADRVQEVAERGTALLLA"
     gene            complement(714..1589)
                     /gene="nadC"
                     /locus_tag="PLX85_06655"
     CDS             complement(714..1589)
                     /gene="nadC"
                     /locus_tag="PLX85_06655"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRC62887.1"
                     /translation="MLDEVLPPDPREVMRIVRRALAEDRAYHDVTTRATVPSDQRGRG
                     AFLVKAPGVICGMEVVRAVFAEMSDELELEVFAPDGSYAEVRHVAAEVRGPLAAMLSG
                     ERVALNLLQRMSGIATMTKRFVETAAEGGPAQILDTRKTTPGLRELERYAVRVGGGRN
                     HRDTLADGLLIKDNHINAAVKRGVSIPDLIEEVRRTIPHTLRIEIEVTDGAMALLALA
                     SDADTILLDNMSPEEMRQIIDDAPDDGILFEASGGVNLETVREIAASGVHLISVGALT
                     HSAPALDISLEIEAL"
     gene            2047..>2441
                     /locus_tag="PLX85_06660"
     CDS             2047..>2441
                     /locus_tag="PLX85_06660"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRC62888.1"
                     /translation="MSILTRAFARASAHDENVLATPHFEAASCPPIADAPPAEDDATF
                     ASLGISSDLVQALEASGIARPFPVQTLTIPDVLAGLDVCGKAKTGSGKTLAFGLPLIE
                     RTERGSARRRPRSLVLVPTRELANQIADAL"
BASE COUNT          363 a          920 c          768 g          390 t
ORIGIN      
        1 gcccgccgtc gtctccacga ccgccacgcg cgctcctcgc gccgcaagcg ccgcgcctgc
       61 cgcgcctgcc agttcgtccg tttcgagggg gaacattgct ggcactcgcc tccggcctcc
      121 agtctaggcg gaagcgacac ggtcgaggtc aggcgaggag gagcgcggtg ccgcgttcgg
      181 ccacttcctg cactcgatcc gccgtaagcg cagcatctcg gtcgcccgag gagtccagca
      241 ccgttcccgg cagcgccccc gactccgcct cggaggcgag ccgttcgtgc agcacgtgca
      301 cgcgctcggg gatgctgaac cgtctcgatg cgtcacgcgc cagcgtgacc tcgatcgtcg
      361 gcaacagggt tacgaggtgg accgggacgc ccgcgccggc gagcgcctcg tggtacacct
      421 tggccatctc tgccggcact acgtcgtcga tcacgaccgc gtagcgcatc gccaccgact
      481 cccgcgcgat cgcggaagcg ttgcgtgcgg ccagcacgag ctgctcgtgc gcctgggcgt
      541 caccggtcca ggggtggcgg tagcccgcct tcaccatgtg ccgcagctcg tccacctcga
      601 catgcagcat gcggtcgtag cgctcgcaga gcgcctcggc gacggcggtc ttcccggccc
      661 cgggtggccc gctgaggatg atgacgtgct ggctcacgcg cgactccgag gcctcacagc
      721 gcttcgatct caagcgagat atcgagcgcc ggcgccgagt gcgtgagcgc cccgaccgaa
      781 atgaggtgca cccccgacgc cgcgatctcg cgcaccgtct cgaggttcac tccgccagac
      841 gcctcgaaca ggatcccgtc gtccggcgcg tcgtcgatga tctgccgcat ctcctccggc
      901 gacatgttgt cgagcaggat cgtgtccgcg tctgaggcga gcgcgagcag cgccatcgcg
      961 ccgtccgtca cctcgatctc gatgcgcaac gtgtggggga tggtgcgtcg cacctcctcg
     1021 atcaggtcgg gaatcgagac gccccgcttc actgcggcat tgatgtggtt gtccttgatc
     1081 agcaagccat cggcgagcgt gtcgcgatgg ttgcgcccgc cgcccacgcg caccgcgtag
     1141 cgctccagct cccgcagccc gggcgtcgtc ttccgcgtat cgaggatctg cgctgggccg
     1201 ccctcggccg ccgtctcgac gaaccgcttc gtcatcgtcg cgataccgga catccgctga
     1261 aggagattga gcgcgacgcg ctcccccgag agcatcgccg cgagcggccc ccgcacctcg
     1321 gcggcgacgt gccgcacctc ggcgtacgag ccgtcgggag cgaatacctc cagctccagc
     1381 tcgtccgaca tctccgcgaa caccgctcgc acgacctcca tgccgcagat cacgcccggc
     1441 gccttgacga ggaaggcgcc ccgtccgcgc tggtcagacg gcaccgtcgc gcgcgtcgtc
     1501 acgtcgtggt acgcgcggtc ctcggcgagc gcccgccgga cgatgcgcat gacctcgcga
     1561 gggtcgggcg gcagcacctc atcgagcatc gaagcctcct gtcggggtcg atactacccg
     1621 agccttgccg gggtggtcag gaggctcacg agacgggagt tatccaataa ccatcgaacg
     1681 ttgttgataa ctgttaagta atcccctgac ctcggcccct tgctcctacg gctggtccac
     1741 cccgcgagtt gttcgccacg gccgatgagc gctcccggcc aacacgtgct acggtggaaa
     1801 caccacgccg aggacgtacg aacggtccga ggcgtccctc agctacgact cttctctcac
     1861 ccgccgcgcg tacgtcgcac atgtgagctc ggaagagcgc ctcgcccagg cggtcgaggt
     1921 gcggtgactg agactcacga gcagtgccgg cccatggctg ttgggcctcg tccccacgta
     1981 ccgctggcag accgccgcgc ccgggaccgc tctctcaacc gcagccgcac gagatggaga
     2041 ccctgtttgt cgatcctgac gcgagcgttt gcccgcgcct ccgcccatga cgagaacgtc
     2101 ctcgccaccc cacacttcga ggcggcatcg tgcccgccca tcgcggacgc cccgcccgct
     2161 gaggacgacg ccaccttcgc gtccctcggc atctcctccg acctcgtcca ggcgctggaa
     2221 gcgagcggca tcgcccgccc gttcccggtg cagacgctca cgatccctga cgtcctcgcg
     2281 ggtctcgatg tatgcggcaa ggcgaagacc ggctctggca agaccctcgc tttcggcctg
     2341 cccctgatcg agcgcaccga gcgcgggagc gctcgtcgcc gtccgcgctc tctcgtgctc
     2401 gtgcccaccc gcgagctggc caatcagatc gcggacgccc t
//
LOCUS       DAORPG010000280         2429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4951_length_2429_cov_3.149115, whole genome shotgun sequence.
ACCESSION   DAORPG010000280 DAORPG010000000
VERSION     DAORPG010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4951_length_2429_cov_3.149115"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLX85_06665"
     CDS             complement(<1..189)
                     /locus_tag="PLX85_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62889.1"
                     /translation="MPSAAERRRVAVLWHGDPAAPPPPGETRYARIFETLEDAGLAPE
                     PVVYADSAIEAVRARLLAC"
     gene            complement(191..631)
                     /locus_tag="PLX85_06670"
     CDS             complement(191..631)
                     /locus_tag="PLX85_06670"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HRC62890.1"
                     /translation="MRRIVRSLPLVLAAFAALALLAACGGDDKDEAPARAMQVSMQDI
                     AYSNIALTASKGEHIRIELDNKGQVTHDFTVDGIPMQGLHMMGGASGGDHQHMAADHA
                     MHLALDKGQSGSLEFTPTEAGTYEFYCTVAGHRDAGMHGTLTVQ"
     gene            718..1425
                     /gene="nth"
                     /locus_tag="PLX85_06675"
     CDS             718..1425
                     /gene="nth"
                     /locus_tag="PLX85_06675"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRC62891.1"
                     /translation="MAGRRHSSAAGSESTGLYAPSELIAILDAYYGRAPAQASGDPMA
                     ELVLTLLSQHTSDHNSGRAFHRLLERFPEWDGVIAARTSDVEEAIRPGGLAPTKAPRL
                     QELLAAVRELTPEYDLGFLETMPLEEAKAWLRALPGVGPKTAACVLLFALGRPALPVD
                     THVDRVSRRLGLVPPKASVEQAHVILEGMLAPDEVYAFHVDLIQHGRRTCHARGPECA
                     RCPLLERCPRVGLPPLG"
     gene            1537..2271
                     /locus_tag="PLX85_06680"
     CDS             1537..2271
                     /locus_tag="PLX85_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62892.1"
                     /translation="MVAAAFVALALALVTSAGGLASTFYPAGSTGFDVSYPQCGATLP
                     RGGAFGIVGVTNGLPWSANPCLATEYQWARGASSGASFYMNTANPGPISKYWNRPGPR
                     TCADATSYSDVGCAYNYGWNAANDAWTVANGATSGAATGRYWWLDVETVNSWNGTTAA
                     NGEAILGYLEALRSLGAGGVGIYSTRYQWTTITGGRQFSSTPNWVAGALNARDAAQRC
                     SSSFAGGPVWLVQYISRGVDSDFVCR"
BASE COUNT          319 a          718 c          996 g          396 t
ORIGIN      
        1 acaggcgagg aggcgtgcgc ggaccgcctc aatggcggag tcggcgtaga cgacaggttc
       61 gggcgcgagt ccggcgtcct cgagggtttc gaagatccgc gcgtagcggg tttccccggg
      121 aggcgggggc gcggccgggt cgccgtgcca gaggacggcg acgcgacgac gctctgccgc
      181 cgagggcacg ttactggacg gtgagcgtgc cgtgcattcc ggcgtcgcgg tggccggcga
      241 cggtgcagta gaactcgtag gtcccggcct cggtgggggt gaactccagg gagccggact
      301 ggcccttgtc gagggcgagg tgcatggcgt ggtcggcggc catgtgctgg tgatcgccgc
      361 cgctggcgcc gcccatcatg tggaggccct gcatcgggat gccgtcgacc gtgaagtcgt
      421 gggtgacctg gcccttgttg tcgagctcga tgcggatgtg ctcacccttc gaggcggtga
      481 gggcgatgtt cgagtaggcg atgtcctgca tcgagacctg catggcgcgc gcgggcgcct
      541 cgtccttgtc gtccccgccg caggcagcca ggagtgcgag ggcggcgaac gcggcgagga
      601 caagaggcag ggagcggact attcggcgca tggggcgatc ctttcggctg cggacattgt
      661 catgcatggg cacggtgtag cgctgccggc gaggctggcg cgggctacga tgcgcgcatg
      721 gcggggcgtc gacattcaag tgcggcggga agcgagagca cggggctgta cgccccatcc
      781 gagctgatcg cgatcctcga tgcgtactac gggcgggcgc cggcgcaggc ctcgggggac
      841 ccgatggcgg agctggtgct gacgctgctg tcgcagcata cgtccgacca caactcgggg
      901 cgggcgttcc accggctgct ggagcggttc ccggagtggg atggggtgat cgcagcgcgc
      961 acctcggacg tggaggaggc gatccggccg ggcgggctgg ctccgacgaa ggccccgcgg
     1021 ctgcaggagc tgctggcggc ggtgcgggag ctgacgccgg agtacgacct cgggttcctc
     1081 gagacgatgc cgctcgagga agcgaaggcg tggctgcggg cgctgccggg agtgggaccg
     1141 aagacggcgg cgtgcgtgct gctgttcgcg ctcgggcggc cggcgctgcc ggtggacacc
     1201 cacgtggacc gggtgtcgcg acgactggga ctggtgccgc cgaaggcgag cgtggagcag
     1261 gcgcacgtca tcctcgaggg gatgctggcg ccggatgagg tgtatgcgtt tcacgtggac
     1321 ctgatccagc acgggcggcg gacctgtcat gcccgggggc cggagtgtgc tcgatgcccg
     1381 ttgctcgagc ggtgtccgcg ggtgggcttg ccgccgctcg gatgaccgga cttcgagtcg
     1441 gtgcgacggg cggctggtat gctgcgggcg ctgtcgagcc tcgacagatc cgggagtgag
     1501 ggaagcatgg cgcgccgttc gacttcgatg cgtctggtgg tggcggctgc gttcgtggcc
     1561 ctggcgctgg cgcttgtgac ttcggccggc ggcctggcga gcacgttcta tccggcgggc
     1621 agcacggggt tcgacgtgtc gtacccgcaa tgcggcgcga cgctccctcg aggcggggcg
     1681 ttcgggatcg tgggcgtgac gaatgggctg ccgtggtcgg cgaacccgtg cctggcgacg
     1741 gagtaccagt gggcgcgtgg tgcctcctcc ggggcgtcct tctacatgaa tacggcgaac
     1801 ccgggtccga tcagcaagta ctggaaccgg ccgggacctc ggacgtgcgc cgacgcgacg
     1861 tcgtattcgg acgtggggtg tgcgtacaac tacggctgga acgcggcgaa cgacgcgtgg
     1921 accgtggcga acggcgcgac ctcgggggcc gcgaccgggc ggtactggtg gctggacgtg
     1981 gagaccgtga acagctggaa cgggacgacg gcggcgaacg gtgaggcgat cctggggtac
     2041 ctcgaggcgc tgcggagcct cggcgcgggc ggggtcggga tctactcgac gcgttaccag
     2101 tggacgacga tcacgggcgg ccggcagttc tcgtcgacgc cgaactgggt ggcgggggcg
     2161 ctcaatgcgc gggatgcggc gcagcgctgc agctcgagct tcgccggcgg gccggtatgg
     2221 ctggtgcagt atatttcgcg cggcgtagac agcgattttg tctgccggta gggccgtaac
     2281 gcctcggcgg gcggttgtgc cctgtccctt cggctccgtc tcgcacttcg gggaggcggc
     2341 tggtgtggac gggccattgc gcggcccggc tgttgttgtc tgggaggccg tccgtccgcc
     2401 gctcaggggc cttcgtcccc tgaaccccc
//
LOCUS       DAORPG010000281         2428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4957_length_2428_cov_4.215339, whole genome shotgun sequence.
ACCESSION   DAORPG010000281 DAORPG010000000
VERSION     DAORPG010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4957_length_2428_cov_4.215339"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PLX85_06685"
     CDS             complement(<1..470)
                     /locus_tag="PLX85_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PtsA"
                     /protein_id="HRC62893.1"
                     /translation="MTSIVEASDMRRRRRHFIDRTMTGVMVGAAVVGVIPLVLILFYL
                     LKEGIPGLSLSLFTEDPNRPGLPGGGVRNSIVGSAIMVTVATLIGVPIAVGCGIFLSE
                     FGQNRLGTVLRFLVDVLAGIPSITIGLFIYGLMVVTRGEFTGFAGGLALSILFLP"
     gene            complement(470..1438)
                     /gene="pstC"
                     /locus_tag="PLX85_06690"
     CDS             complement(470..1438)
                     /gene="pstC"
                     /locus_tag="PLX85_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRC62894.1"
                     /translation="MAGLAQERGRTSLVNRVRIGDWIFTALVSAAAAVVLLIIVGLFY
                     ELITRSWPAVTHNGIDFIWATEWNPPREVFNAGTFIVGTVVTSVAALVIATAIGVLVA
                     IYLVEIAPPMVARPVSFMVELLAAIPSVVFGLWGVFVLGVFIRDDFGPRFVDVFGFIP
                     FFDGTPLITGLLSAVLILATMILPTVMAVSRDVIAAVPRSQREGIVALGATRWEMIQL
                     TVLPYARSGVLGAAILGLARAVGETLAVTLVIGNRVGIPHTIFDPAYTMASAIANQFT
                     EVENDMHRASLLEVALLLLIITFLINVAARLLVWRVTGGQRVSVVE"
     gene            complement(1518..>2428)
                     /gene="pstS"
                     /locus_tag="PLX85_06695"
     CDS             complement(1518..>2428)
                     /gene="pstS"
                     /locus_tag="PLX85_06695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HRC62895.1"
                     /translation="AEVAKGVQVNYQSIGSGGGIQQFTEKTVDFGASDAPMSDAELAK
                     ATDAVHIPMVLGSVVLTYNVSGVNTPLKFDGDTIAKIYMGTIKKWNDPAIAALNSGMK
                     LPDADIQVVYRSDGSGTTFVWTDYLSSVSADWKSKVGTSKNPSWPVGQGGKGNEGVTT
                     AVKQTPNAIGYVELNYAIANKLAFADIKNKAGKFITPSIDATSAAAAGVTIPDDYRVS
                     IVNASGEGAYPIASFTYLLVHKDTQSCDKQKPLVNMLWWVFHDKNANETAKSLNYAPL
                     PATLQPKVDATLKTLTCNGQPILPKG"
BASE COUNT          429 a          714 c          891 g          394 t
ORIGIN      
        1 ggcaggaaca ggatcgagag ggcgaggccg ccagcgaagc cggtgaattc gccgcgggtg
       61 acgaccatga ggccgtagat gaagaggccg atcgtgatcg aggggatgcc ggcgagcaca
      121 tcgacgagga agcggagcac ggtaccgagg cggttctgtc cgaactcgga gaggaagatc
      181 ccgcagccca cggcgatggg gactccgatg agcgtggcga cggtcaccat gatggcgctg
      241 ccgacgatcg agttgcggac gccaccgccg ggcaggccgg gacggttggg atcctcggtg
      301 aagagactga ggctcaggcc cggaatgccc tccttcagca ggtagaacag gatgagcacc
      361 aacgggatca ccccgaccac cgcggcgccg accatgacgc cggtcatcgt gcggtcgatg
      421 aagtggcggc ggcgccgccg catgtccgag gcttcgacga tgctcgtcat cactccacca
      481 ccgatacgcg ctggccgccg gtgaccctcc agacgaggag gcgggcggcg acgttgatca
      541 ggaacgtgat gatgaggagc agcagcgcga cctcgagcag ggaggcgcgg tgcatgtcgt
      601 tctcgacctc ggtgaactgg ttggcgatgg cgctggccat cgtgtaggcg gggtcgaaga
      661 tggtgtgggg gatgccgacg cggttgccga tgacgagggt gaccgcgagg gtctcgccga
      721 cagcgcgggc gagaccgagg atggcggcgc ccagcacccc cgaacgggcg tacggcagga
      781 cggtcaactg gatcatctcc cagcgagtcg cgccgagggc cacgatgccc tcgcgctgac
      841 tgcgggggac ggcggcgatg acgtccctcg acacggccat gacggtgggg aggatcatcg
      901 tcgcgaggat gaggacggcg gagagcaggc cggtgatgag cggggtgccg tcgaagaagg
      961 ggatgaagcc gaagacatcg acgaagcggg ggccgaagtc gtcacggatg aagacgccga
     1021 ggacgaatac gccccagagc ccgaagacga cgctggggat ggccgcgagc agctccacca
     1081 tgaacgaaac gggccgggcg accatcggcg gggcgatctc gacgaggtag atcgcgacga
     1141 gcacgccgat cgcggttgcg atgacgagcg cggccaccga ggtgaccacg gtcccgacga
     1201 tgaacgtgcc ggcgttgaag acctcacggg gcgggttcca ctcagtcgcc cagatgaagt
     1261 cgatgccatt gtgggtgacg gccggccagg agcgcgtgat gagctcgtag aagaggccaa
     1321 cgatgatcag caagacgacc gccgcggcgg ccgagaccag cgccgtgaag atccagtcgc
     1381 cgatccggac acggttgacc aggctggtcc gaccgcgttc ctgtgcgagt cccgccatag
     1441 ttacctcgct tcaggccgag gggccggagc gttcggcttc cggcccctcg accgttttcc
     1501 ggagtcgatc ctggcgacta gcccttcggg aggatgggct ggccgttgca ggtcagggtc
     1561 ttgagggtgg cgtccacctt cggctgcagc gtagcgggga gcggggcgta gttgagcgac
     1621 ttcgcggtct cgttggcgtt cttgtcgtgg aacacccacc acagcatgtt caccagcggc
     1681 ttctgcttgt cgcagctctg cgtgtccttg tggaccagga gataggtgaa cgaggcgatg
     1741 gggtacgcgc cctcgccgct ggcgttgacg atcgagacgc ggtagtcgtc cgggatggtc
     1801 acccccgcgg cggcggcgct ggtggcgtcg atggacgggg tgatgaactt gccggccttg
     1861 ttcttgatgt ccgcgaacgc gagcttgttg gcgatggcgt agttgagctc gacatagccg
     1921 atggcgtttg gggtctgctt cacagcagtg gtcacgccct cgttgccctt gccgccctgg
     1981 ccgacgggcc agctcgggtt cttgctcgtg cccaccttgc tcttccagtc agcgctgacg
     2041 ctggacaggt agtcggtcca gacgaaggtc gtgccagagc catcggagcg gtacaccacc
     2101 tggatgtcgg cgtcgggcaa cttcattccc gagttcagcg cggcgatcgc ggggtcgttc
     2161 cacttcttga tcgtgcccat gtagatcttc gcgatggtgt cgccgtcgaa cttcagggga
     2221 gtgttcacgc ccgaaacgtt gtaggtgagg acgacggagc cgagcaccat cgggatgtgg
     2281 acggcatcgg tggctttggc gagctcagcg tcgctcatcg gagcgtccga ggcgccgaag
     2341 tccaccgtct tctcggtgaa ctgctggatg ccgccgcccg acccgatcga ctggtagttg
     2401 acctggacgc ccttggcgac ctcggcct
//
LOCUS       DAORPG010000282         2427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4966_length_2427_cov_3.996627, whole genome shotgun sequence.
ACCESSION   DAORPG010000282 DAORPG010000000
VERSION     DAORPG010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4966_length_2427_cov_3.996627"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLX85_06700"
     CDS             complement(<1..346)
                     /locus_tag="PLX85_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC62896.1"
                     /translation="MTARTAPARTGFDPRWLPLAVTTIGSFMSLLDSTIVNIALPTIL
                     RDFGASLESGQLVLTVYLVALAVVIPVSGYLGERFGMKRLYIVTLILFTAGSTLCGFA
                     WNLPSLIVFRVLQ"
     gene            501..791
                     /locus_tag="PLX85_06705"
     CDS             501..791
                     /locus_tag="PLX85_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62897.1"
                     /translation="MPLSQRSLSCLAFAMFALMWLAAIAQSPGGMTAFAHELVTVEQE
                     LVPPPPPEQPQQGTKEQGQLETYPGGILCDEEPDAQGTLHTECQAPALMYPY"
     gene            1335..2264
                     /locus_tag="PLX85_06710"
     CDS             1335..2264
                     /locus_tag="PLX85_06710"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRC62898.1"
                     /translation="MAVFTGNAHRAFAEAVCQHLEMPLGECEVFEFSNENIFVKFLEN
                     IRERDVFLIQPIVPPVNTRLMELLIMIDAAKRASAGRITAVIPYYGYGRSDKKDQPRV
                     PITARLIANFIEVAGANRVLTVDLHAGQIQGFFNIPLDELTALPLLADYFHTLRLPAP
                     TVVATDVGGAKGARDVARRLDCDLAIVDKRRTDNNERAEAMTLIGEVEGRDCIIVDDE
                     ILTGGTMVSSVRLLRDRGARSVYVSCIHPIFAPRAYDLLDTKEIAELVFTDTVPLRQP
                     FEHVPTRTLSLAPLFGDAMHRIHTGGSVGELFL"
BASE COUNT          430 a          843 c          757 g          397 t
ORIGIN      
        1 cctgtagcac gcggaacacg atcaggctcg gcaggttcca tgcgaagccg cagagcgtcg
       61 aaccagccgt gaacaggatc agcgtcacga tgtagaggcg cttcatcccg aagcgttcgc
      121 ccaggtaccc gctcaccggg atcacgaccg ccagcgccac caggtacacc gtcagcacca
      181 gctggccgct ttcaaggctc gctccgaagt cccgcaggat ggtcggcagc gcgatgttca
      241 cgatcgtcga gtccagcagg gacatgaacg agccgatcgt ggtcaccgcg agcggcagcc
      301 agcgcgggtc gaagccggtg cgagcgggtg cggtccgagc ggtcatgcgg cctcctctgt
      361 cctcgttaca gttaatcata cagtgctgcc gaactgtatt gcgcagtcgt acaatgtgtt
      421 gagatgaaac tgctagcgag ggccgtcgcc cgccacccgg cgcacgcgcg cccccgcgac
      481 gctggctctg gaggtgacgc atgccgctgt cgcagcgatc cctctcctgc ctcgccttcg
      541 cgatgttcgc cctcatgtgg ctggctgcca tcgcccagtc gcccggcggc atgaccgcgt
      601 tcgcccacga gctcgtcacc gtcgagcagg aactcgtgcc cccgccgccg cccgagcagc
      661 cccagcaggg cacgaaggag cagggtcagc tcgagaccta ccccggcggc atcctctgcg
      721 acgaggaacc ggacgcgcag ggcacgctcc acacggagtg ccaggcgccc gcgttgatgt
      781 acccctactg acgccgccgg gcttcaggcg gcgcgcggaa tgggtcagtg tgaatcccta
      841 aacaacgcaa cagccatccc gatggtcatg agaacaccag cagcgacagt gatggccgcc
      901 atcgctcttc tggcttctgc actcatgaac cgagaagcgc cgttcccgcg ccaaatcgaa
      961 tcatccgggg tagcagggtt gcgccattga gatacaccca acccgacgag agtcaccccg
     1021 acgagtatca tgaagacctt cgggggagcg gcggcggggt caatttttct ctcaggattc
     1081 atgcaatcgc ctcgccgctg ctgctgatgc ggcccgcgtg ggtatcgggc cgtccaatca
     1141 agactaccaa cccgtggact cggcagcggc gatcagggac gctgcgctgc ggcggccggg
     1201 cggcggcctc gctcagggcg cgatcctcag actgacccac cagcccgcgc cccgcctcga
     1261 taggttcagt ctgaggttgg tgctaccatg aacgttcccc accagccgca gaggagtacg
     1321 acgtcgtgcg cgagatggcg gtgtttactg gcaacgcgca tcgcgcgttc gcggaggccg
     1381 tgtgccagca cctcgagatg ccgttggggg agtgcgaggt gttcgagttc tcgaacgaga
     1441 acatcttcgt caagttcctc gagaacatcc gcgagcgtga cgtcttcctg atccagccca
     1501 tcgtgccgcc ggtgaacacg cggctgatgg agctgctgat catgatcgac gcggccaagc
     1561 gggcttcggc cggccgcatc accgccgtca tcccgtacta cgggtacggc cgctccgaca
     1621 agaaagacca gccgcgcgtc cccatcaccg cccgcctgat cgcgaacttc atcgaggtcg
     1681 ccggcgccaa tcgcgtgctg acggtcgacc tccacgccgg ccagatccag ggcttcttca
     1741 acatccccct ggacgagctg accgccctcc ccctcctcgc ggactacttc cacactctgc
     1801 gcctcccggc gcccacggtc gtcgcgaccg acgtcggagg cgccaagggt gcgcgcgacg
     1861 ttgcccgccg tctcgactgc gacctcgcca tcgtcgacaa gcgccgcacg gacaacaacg
     1921 agcgtgccga ggcgatgacg ctcatcggcg aggtcgaggg ccgcgactgc atcatcgtcg
     1981 atgacgagat cctcacgggc ggtaccatgg tatcgagcgt gcgtttgctt cgagaccgcg
     2041 gcgcgcgctc ggtctacgtc tcctgtatcc acccgatctt tgcgcccagg gcgtacgacc
     2101 tgctggatac gaaagagatc gccgagctgg tgttcacgga tacggtgccg ctgcgccagc
     2161 ccttcgagca cgtgccgacg cggacgctct cgctggcgcc cctgttcggc gatgccatgc
     2221 accgtattca caccggcgga tcggtcggtg agctgttcct gtagcccggc tgcgggacgg
     2281 cggtcaggag cgacatgctc aacgtcttta agaagctgat cggcgacgcc aacgagcgcg
     2341 ccctcaagga tctctggcca ctcgtcgacg aggtcaacga gtacgccgac gagatggcgg
     2401 ccctcgacga ggccggcctc cgcgccg
//
LOCUS       DAORPG010000283         2427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4968_length_2427_cov_3.212479, whole genome shotgun sequence.
ACCESSION   DAORPG010000283 DAORPG010000000
VERSION     DAORPG010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4968_length_2427_cov_3.212479"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /gene="murB"
                     /locus_tag="PLX85_06715"
     CDS             complement(<1..848)
                     /gene="murB"
                     /locus_tag="PLX85_06715"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRC62899.1"
                     /translation="MDRPPPPAGIEIHEHAPLARHTYMRIGGPAEYFATPADLVELER
                     LMLWARAAAMPMRIIGGGSNLLVADEGVSGLVVSLRRACGGIEFTGSRVTAGAAVMLP
                     ALARAAAERGLGGLEFAVGIPGSVGGALQTNAGIGDGRCIGPLVREVEVFGGRGFQTL
                     SRYEIHFDYRHSSLRGAGVMVVGATLHLEPRPTAEIQAEMRQLLEARSASQPTAEPNA
                     GSMFRNPEGNAAGRLIEAAGCKGLASGGVRVSELHANFFVHDGSGRASDVLALMSEVQ
                     RRVLETS"
     gene            946..1647
                     /locus_tag="PLX85_06720"
     CDS             946..1647
                     /locus_tag="PLX85_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC62900.1"
                     /translation="MSKVLVVEDDPAILDAVAYNLKRDGHEVLTATDGLDGLQLAREA
                     GPDLVLLDLMLPRMSGLDVCRILRSERPVPILMLTARDTEVDKVVGLELGADDYLTKP
                     FSMRELMARVAAVLRRDRMSREAGHETPSARSETIEGGDIALNISAHEARRAGEVVPL
                     RPKEFDLLEFLMRHPGQVLTREMILETVWGYSYGGETRTVDVHIRWLREKLEADPAHP
                     AHIQTVRSVGYKFVP"
     gene            1644..>2427
                     /locus_tag="PLX85_06725"
     CDS             1644..>2427
                     /locus_tag="PLX85_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRC62901.1"
                     /translation="MTPAWRSPAAVALLALLAAASVLLLPAAVAAVVVLTIVALAAVA
                     ARGDDHEPPREAAPVVSPPEGLGRAIAPLATGVLALDGDRNVLLANPAAASILARPLD
                     SMIAVSLMQAVRDHDLAEVARQATGLPIEVHLSTANRDVLATATPVEFDAVRTLLAIE
                     DVTELRRAQRARSELVANVSHELRTPIAAARALAETLETGVAEEDDRQRFLRRLLEEI
                     DRLSAIVQRLLWLARVESGAGTFSPTPLDATDLLTESTERVAP"
BASE COUNT          359 a          968 c          746 g          354 t
ORIGIN      
        1 gacgtctcga ggacccgccg ttgcacctcg gacatgagcg ccagcacgtc tgacgcccgt
       61 ccgctcccgt cgtgcacgaa gaagttcgcg tgcagttcgc tcacgcgcac cccgccggac
      121 gccagtccct tgcatccagc ggcctcgatc agccgcccgg cagcattccc ctccgggttg
      181 cgaaacatcg aacccgcgtt cggttccgcc gtcggctgcg aggcggaccg cgcctcgagc
      241 agctgacgca tctccgcctg aatctcggcc gtcggacgcg gttcgaggtg cagcgtcgcg
      301 ccgacgacca tcacgccggc cccgcgcagg ctcgaatgcc ggtagtcgaa atggatctcg
      361 tagcggctca gggtctgaaa cccgcggcct ccgaagacct cgacttcccg caccagcggc
      421 ccgatgcatc ggccgtcgcc gatgcccgcg ttggtctgca gcgcgcctcc aaccgacccc
      481 gggatcccaa ccgcaaactc gaggcccccg agcccgcgtt cggccgccgc ccgcgccagc
      541 gccggcaaca tcaccgccgc gcccgccgtc acccgtgagc cagtgaattc gatgccaccg
      601 caggcccgcc gcagcgagac gacgagtccg ctcacgcctt cgtcggccac gagcaggttc
      661 gaccctccac cgatgatccg catcggcatc gcggcagctc gggcccacag catcagccgt
      721 tcgagttcga cgaggtccgc gggtgtagcg aaatactccg ccggcccccc gatacgcatg
      781 taagtgtgcc gggcaagcgg agcatgctcg tgaatctcga tgcccgcggg aggcggaggc
      841 cggtccatcc ctcgatggta gggcgtgcac ccgacgcgca cgagagcgcg cggatgctcc
      901 ccgtggccgg ttgccatcct ccagcccgcc atcgacactg gcgccatgag caaggtcctc
      961 gtggtcgagg atgatcccgc gattctcgac gccgtcgcct acaacctgaa gcgcgacggc
     1021 cacgaggtgc tgaccgctac cgacggcctc gacggcctgc agctcgcccg cgaggccggc
     1081 cccgacctcg tgctgctcga cctcatgctg ccccgcatgt ccggcctcga tgtctgccgc
     1141 atcctgcgct ccgagcgccc cgtcccgatc ctcatgctca ccgcgcgtga caccgaggtg
     1201 gacaaggtcg tcgggctcga gctcggagct gacgactacc tgacgaagcc tttttcgatg
     1261 cgtgagctga tggcgcgcgt cgccgccgtc ctgcgccgcg accgcatgtc acgggaggcg
     1321 ggccacgaga ccccgtctgc ccgcagcgag accatcgagg gcggcgacat cgcgctgaat
     1381 atctcggccc acgaagcccg gcgcgcgggc gaggttgtcc ccctccgccc caaggagttc
     1441 gacctgctcg agttcctgat gcgccacccc gggcaggtgc tcactcgcga gatgatcctc
     1501 gagaccgtct ggggctacag ctacggcggc gagacgcgaa cggtcgacgt ccacatccgc
     1561 tggctgcgcg agaaactcga ggcggatccc gcccatcctg cccacatcca aaccgtgcgc
     1621 agcgtcggct ataaattcgt accgtgaccc ccgcctggag gtcgcccgcc gcggtcgctc
     1681 tcctcgccct cctggctgct gcctccgtcc tcctgctgcc cgccgcggta gccgccgtgg
     1741 tcgtgctcac catcgtcgcg ctggcggcgg tcgccgcccg cggcgacgat cacgagcccc
     1801 cacgcgaggc cgccccggtc gtgtcgccac ccgaagggtt gggccgcgcc atcgcgcccc
     1861 tcgccaccgg cgtcctcgcg ctcgacggtg accgcaacgt cctgctcgcc aaccccgccg
     1921 ccgcctccat cctcgcccga ccgctcgatt cgatgatcgc cgtctcgctc atgcaggccg
     1981 tccgcgatca cgaccttgcc gaggtcgccc gccaggccac cggccttccg atcgaagtcc
     2041 acctctccac cgcgaaccgc gatgtcctcg cgacggcgac cccggtcgag ttcgacgccg
     2101 tgcgcacgct gctcgccatc gaggacgtca ccgagctacg tcgggcccag cgcgcgcgca
     2161 gcgagttggt cgccaacgtc tcacacgagc tgcggacccc catcgcggcc gcccgcgcgc
     2221 tcgccgagac cctcgagacg ggcgtggccg aggaggacga ccggcagcgg ttcctccgcc
     2281 gcctcctcga ggagatcgac cgcctcagcg caatcgtcca gcggctcctc tggctggccc
     2341 gcgtcgaatc cggggcgggg acgttcagcc ctacgccgct ggatgccacc gaccttctca
     2401 ccgagtcaac ggaacgagtc gcccccg
//
LOCUS       DAORPG010000284         2424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_4983_length_2424_cov_2.926129, whole genome shotgun sequence.
ACCESSION   DAORPG010000284 DAORPG010000000
VERSION     DAORPG010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4983_length_2424_cov_2.926129"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PLX85_06730"
     CDS             complement(<1..483)
                     /locus_tag="PLX85_06730"
                     /inference="COORDINATES: protein motif:HMM:NF028169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRC62902.1"
                     /translation="MNEEQAGPPARRPGRPRSAEAERAILDATLELLAEQGLEGVTME
                     AAAARAGVSKATIYRRWRSRLEMIEAAFQSIDREVRIVETGSVRGDLVTIIGEFGRAT
                     ARTLPPPALAQMIAVTLATPELRQLFLRHVFEVRRAAMIAALRRGQERGEIRTDIDLN
                     L"
     gene            complement(480..1214)
                     /locus_tag="PLX85_06735"
     CDS             complement(480..1214)
                     /locus_tag="PLX85_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC62903.1"
                     /translation="MSPRITLWTARRVLMQLRHDRRTVGMMLLVPAILMTLLRFIYDA
                     KPQVFDHVGLTLLGVLPFTTMFLITSIGMLRERTSGTLERLLTTPMAKLDLLLGYGIA
                     FAVAAALETTVAAVVAFVFLGLDTAGSPFLVGVIAVANAVLGTATGLLVSAFAYSEFQ
                     AVQFMPAIVMPQVLLCGLIAPREQMAAPLRFASDLMPMTYAVEALDEVGKGNAGATMA
                     VDVAVIVVVAFAALALGAVTLRRRTA"
     gene            complement(1214..1954)
                     /locus_tag="PLX85_06740"
     CDS             complement(1214..1954)
                     /locus_tag="PLX85_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014692420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC62904.1"
                     /translation="MPQAVATRTAVEVERLVVRRGGKLILDGVSCAIPRGSVVGLLGP
                     SGSGKTTLMRAIVGVQRIASGGVTVLGLPAGSPELRRRVGYLTQAPSIYPDLTVIENA
                     TYFAAMYGCGTAEALASVESVGLTEAAGQLASTLSGGQRARASLACTLLGDPELLVLD
                     EPTVGQDPVLRNELWLRFRALAEAGATLIVSSHVMDEARRCDRLLFISDGELIADGTP
                     EEVLQRTATDDLDEAFLRLVIAHREEQD"
     gene            2125..>2424
                     /locus_tag="PLX85_06745"
     CDS             2125..>2424
                     /locus_tag="PLX85_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013914811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC62905.1"
                     /translation="MPETAQLRAEPGSVAAIRDVAGRIRTSVARVIVGAEEPIDLILA
                     ALFSGGHILIEDVPGTGKTMLARAFARSIGATFHRIQFTPDLMPSDILGINYYSQR"
BASE COUNT          391 a          935 c          741 g          357 t
ORIGIN      
        1 caggttcagg tcgatgtccg tgcggatctc gccgcgttcc tggcctcgtc gcagcgcggc
       61 gatcatggcc gcccgccgca cctcaaacac gtggcgaagg aagagctgcc gcaactccgg
      121 agtcgcgagg gtgaccgcga tcatctgtgc cagggcgggc ggcggcagtg tgcgcgcggt
      181 tgcccggccg aactcgccga tgatcgtcac gaggtcacct cgcacggagc ccgtctcgac
      241 gatgcgcacc tcccggtcga tggactgaaa ggccgcctcg atcatctcga gacgcgaccg
      301 ccagcgccgg tagatcgtcg ccttgctcac ccccgcccgc gcggccgccg cctccatcgt
      361 cacgccctcc aggccctgct ccgcgagcag ttccagcgtg gcatcgagga tcgcccgttc
      421 ggcttccgcg ctgcgtggac gtcccggacg tcgcgccggc ggcccggcct gctcctcgtt
      481 cacgcggtcc gccggcgcag agtcacggcc ccgagcgcga gcgcggcgaa cgccaccacg
      541 acgatcactg ccacgtccac ggccatcgtc gctccggcgt tccccttccc cacctcgtcc
      601 agcgcctcga cggcgtacgt catcggcatc aggtcgctgg cgaaccgcag cggcgcggcc
      661 atctgctcgc gaggcgcgat caggccgcac agcagcacct gcggcatcac gatcgcaggc
      721 atgaactgca ccgcctgaaa ctcgctgtac gcgaatgcgc tcaccaggag cccggtcgcc
      781 gtgcccagca cggcattcgc gaccgcaatc acccccacga ggaatgggct ccccgccgtg
      841 tccagcccga ggaacacgaa cgcgacgacc gccgcgaccg tcgtctcgag ggccgccgcc
      901 acggcgaacg cgatcccata ccccagcagc aggtccagct tcgccatcgg cgtcgtcagg
      961 agccgctcca gcgtccccga ggtccgctcc cgcagcatcc cgatgctcgt gatcaggaac
     1021 atcgttgtga acggcagcac gccgaggagt gtcagcccca cgtggtcgaa gacctgtggc
     1081 ttcgcgtcgt agatgaaccg cagcaatgtc atcagtatcg ccggcaccag cagcatcatc
     1141 ccgacggtcc gccggtcgtg gcgcagctgc atcagcaccc gtcgtgccgt ccacaacgtg
     1201 attcgaggcg acatcagtct tgctcctccc ggtgcgcgat cacgagccgc aggaacgcct
     1261 cgtcgaggtc gtccgtggcc gtccgttgca gcacctcttc cggcgtcccg tcggcgatta
     1321 gctcgccgtc gctgatgaac agcagccggt cgcaccgccg cgcctcgtcc atcacgtggc
     1381 tggacacaat gagcgtcgcc cccgcctcgg ccagcgcccg gaagcgcagc cacagctcat
     1441 tccgcagcac cgggtcctgt cccacggtcg gctcgtcgag gaccagcagc tccggatcac
     1501 cgagcaaggt gcaggcgagc gatgcccgcg cacgctgccc acccgagagc gtgctcgcga
     1561 gctgtcccgc ggcctcggtg agaccgacgg actcgacgct cgcaagcgcc tcggcggtgc
     1621 cgcacccgta catcgccgcg aagtacgtcg cgttctcgat caccgtgagg tccgggtaaa
     1681 tcgagggcgc ctgggtcaga tagccgacgc gccggcgcag ttccggcgaa ccggcgggca
     1741 ggccgaggac cgtcactccg cccgacgcga tccgctgcac gccgacgatg gcccgcatga
     1801 gcgtggtctt cccgctcccg ctcgggccca gcagccccac cacgcttccg cgcggaatcg
     1861 cacagctcac cccgtcgagg atgagcttgc ctccccggcg tacgacgagt cgctcgacct
     1921 cgaccgccgt ccgagtcgca acagcctgtg gcactagtgc ctccttatcg atacgcgaca
     1981 gtatcgaatt aacagtagtg cggcgagtta cgaaacgcaa gagggtcgaa acggccagcg
     2041 gcacaggcaa ccacccgcct gcgcgcctgc tatcctctcg cgtattccct tgcgtgaaga
     2101 gggacgcgtt cgagggagta ccgcgtgccc gaaacggccc agctccgcgc cgagcccggc
     2161 tccgtcgccg ccatccgcga cgtcgccggc cgcatccgta cgagcgtcgc ccgcgtcatc
     2221 gtgggcgccg aggagcccat cgacctcatc ctcgcggcgc tcttcagcgg cggccacatt
     2281 ctgatcgagg acgtcccggg cacgggtaag accatgctcg ctcgcgcctt cgcccgctcg
     2341 atcggggcga ccttccaccg catccagttc acgcccgacc tcatgccgtc cgacatcctc
     2401 ggcatcaact actactcgca gcgc
//
LOCUS       DAORPG010000285         2414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5020_length_2414_cov_4.098347, whole genome shotgun sequence.
ACCESSION   DAORPG010000285 DAORPG010000000
VERSION     DAORPG010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5020_length_2414_cov_4.098347"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="PLX85_06750"
     CDS             <1..145
                     /locus_tag="PLX85_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRC62906.1"
                     /translation="WLAAQPVVSTIIIGATKPEQIVENAEAIEVELSAEDIAGINAAY
                     QGE"
     gene            268..513
                     /locus_tag="PLX85_06755"
     CDS             268..513
                     /locus_tag="PLX85_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62907.1"
                     /translation="MPTYVRRDATGKINGPHISLPEDPNRTFVGEVHEVLKEIDLLRE
                     SHDITVEVEEWLMRIVDAAVGVGFDYGVAYGKSGEAK"
     gene            658..1488
                     /locus_tag="PLX85_06760"
     CDS             658..1488
                     /locus_tag="PLX85_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC62908.1"
                     /translation="MSGRKPVDGLYELADGVFDFVIEGVIQLNAGIVVGDEAVAVIDT
                     GTTEDDARALLEAVRSVTDLPVRYVINTHHHGDHSFGNWWFLPAVVLGHARCRLRLVG
                     SEGFTHRETMAAHLPMAREQIRAVPLAPPDVTFDGHLDLHLGKNSMRLAYLGRAHTDN
                     DIAIGIDDSGVAFAGDLVESAGPPIVNDGYPAEWGPTLRKLGAAMVQMFVPGHGNVVD
                     GMFVAEQAGAFEELTRVCAQAQTPAEAMHAMPRSVFEVLDFQTAAAVKRYFETVTRPS
                     "
     gene            complement(1472..>2414)
                     /locus_tag="PLX85_06765"
     CDS             complement(1472..>2414)
                     /locus_tag="PLX85_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRC62909.1"
                     /translation="LRVAAIGLIVALTLLVIAGLERSIWLSVALVAVQVGGLLVVIVA
                     GAPHLGDESLREGAGIGGVMSAAALVFFAFIGFDEVVTLSEETRDPARSVPRALLLTL
                     GISTLLYIAVGVVAVSAVGADALGRSDQPLGLVMEQDIGSRAGDVIAAIALASTTNTT
                     LLVLTAGARMIFSMARGNVLPPVFARLTPGARTPWLAALAGAAVAIPFTLSGRIELIA
                     QVTNFAIYVLFVGVNLAVIRLRQRRPDLPRRFRAPFEVRWVPLTAALGVVTSVFLLFY
                     IERAAWVLGLLMVASGVAAWLASPYLGRGGAGPMTDE"
BASE COUNT          448 a          812 c          826 g          328 t
ORIGIN      
        1 ctggctcgcg gcgcagcccg tcgtctcgac gatcatcatc ggcgccacaa agccggagca
       61 gatcgtggag aatgcggagg ccatcgaggt ggagctgagc gccgaggaca ttgcgggcat
      121 caacgcggcc taccaggggg aatagcggcc caggacggcg tgccgatggc catgcggcac
      181 gctcgcggtc ggtgccgtag aatccggcct gctctagctc gaagcggggc ttccagctag
      241 tgtcgtttcc gcgaagagag gactccgatg cccacgtacg tgcgccgcga tgcgaccggc
      301 aagatcaacg gcccgcacat ctcgctgccc gaagacccga accggacatt cgtgggcgag
      361 gtccacgaag tgctcaaaga aatcgacctg ctgcgtgaga gccacgacat tactgtcgaa
      421 gtcgaggagt ggctcatgcg catcgttgac gcggccgtcg gcgtcggatt cgactacggc
      481 gtcgcctacg gaaagagcgg cgaggccaag tagtcctcgc gccacacgcg tctcaactgc
      541 gaggtgccct ccggggcacc tcgtttggtg ctgcgctcct tcgccgggca gaccgccgga
      601 gtacactcgt gcatcgagat agccgcacct ccgcggacgc acgcgggacc gaggacgatg
      661 agcggacgta agccagtcga cgggctgtac gagctggctg acggcgtctt cgacttcgtg
      721 atcgagggcg tgatccagtt gaacgcgggc atcgtcgtgg gcgacgaggc cgtcgctgtc
      781 atcgacaccg gcacgaccga ggacgatgct cgtgcactcc tcgaggcggt tcgcagcgtc
      841 acggacctgc cggtgcgcta cgtcatcaac acgcaccacc acggcgatca ctcgttcggg
      901 aactggtggt tcctgccggc agtggtcctc ggccacgcgc gctgccggct gagactcgtg
      961 ggcagcgaag gcttcacgca ccgcgagacc atggcggcgc acctgccgat ggcgcgtgag
     1021 cagatccggg cggtaccact cgctccacct gacgtgacgt tcgacggcca cctcgacctg
     1081 cacctcggaa agaacagcat gcggctcgcg tacctgggac gtgcgcacac cgacaacgac
     1141 atcgcgatcg gcatagatga ctccggcgtg gcgttcgcgg gcgacctcgt agagtcggca
     1201 gggccgccga tcgtgaacga cggctacccg gccgagtggg gcccgacgct gcgcaagctc
     1261 ggcgccgcga tggtgcagat gttcgtaccc ggccacggca acgtggtcga cgggatgttc
     1321 gtcgccgagc aggcgggggc cttcgaggag ctgacgcgcg tctgcgccca ggcgcagaca
     1381 ccagccgagg cgatgcacgc gatgccccgg tcggtcttcg aggtgctcga cttccagaca
     1441 gccgctgccg tgaagcgcta cttcgagaca gtcactcgtc cgtcataggt cccgcgcccc
     1501 cgcgtccgag gtacggcgac gccagccacg cggcgacgcc ggaggcgacc atgagcaggc
     1561 cgaggaccca tgccgcccgc tcgatgtaga agagcagaaa cacgctggtc accacgccga
     1621 gcgccgcggt gagcggcacc caacgcacct cgaagggcgc gcgaaagcgc ctcggcagat
     1681 ccggtcgtcg ctggcgcagc cggatcacgg cgaggttcac gcccacgaag agtacgtaga
     1741 tcgcaaagtt ggtcacctgt gcgatcagct cgatccggcc acttagggtg aacgggatgg
     1801 cgaccgccgc gcctgccagt gcggccagcc agggggtcct cgcaccgggg gtaagtcgcg
     1861 cgaacaccgg cggaagcacg ttccctcgag ccatgctgaa gatcatccga gctccggctg
     1921 tcagaacgag gagcgtcgtg ttcgtcgtcg aggcgagcgc gatcgcggcg atcacgtcgc
     1981 ccgcccgcga cccgatgtcc tgctccatca cgaggccgag cggctgatcg gagcggccga
     2041 gggcgtccgc gcccaccgcg ctcaccgcga ccacacccac ggcgatgtag agcagcgtcg
     2101 aaatgccgag cgtgagcagc aacgcgcgcg gcacagagcg ggcagggtcg cgcgtctcct
     2161 cggagagcgt cacgacctcg tcaaagccaa tgaaggcgaa gaagacgagg gccgccgcgg
     2221 acatcacgcc gccgatgcca gcgccctcgc gcagcgattc gtcgccgaga tgcggtgcgc
     2281 ccgcgacaat caccacgagc agcccaccga cctgcaccgc gacgagggcg accgacagcc
     2341 agatcgaacg ctcgaggcct gcgatgacga ggagcgtgag cgccacgatg agcccgatcg
     2401 cggcgacgcg cagg
//
LOCUS       DAORPG010000286         2414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5021_length_2414_cov_2.996185, whole genome shotgun sequence.
ACCESSION   DAORPG010000286 DAORPG010000000
VERSION     DAORPG010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5021_length_2414_cov_2.996185"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..2085)
                     /gene="fusA"
                     /locus_tag="PLX85_06770"
     CDS             complement(28..2085)
                     /gene="fusA"
                     /locus_tag="PLX85_06770"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRC62910.1"
                     /translation="MKAYASDQLRNLVLMGHGGSGKTTLAEAMLFVSGAISRMGKVED
                     RNTVSDFDEQEHQHHYSISTSLIPVEWAGTKLNVLDAPGYPDFEGEAIAAAAAADAAV
                     ITVDAVGGIQSGSEVAWDHADEAGIRPRFIIVTRLDRENADFDAVLAALRDRFGTSVV
                     PLAVPIGVAGSFAGVVDLASGTAIRGADGAAGEVPADVAGAVESARDMLVESVAETDE
                     ELLNDYLEGNAIDDARLQAALPEAVRSGSVIPVFPVSGTSAFGVKLVMDAAATLLPSP
                     VGRERLLEGGATVTVDPSGPLVAHVFKTTADPFVGRLTYMRVLSGTLKPDANPYNVQH
                     ATQERLGHLFAMRGKEQIGVPELVAGDIGVAAKLTVTTTGDTFVASEASRGTRVPPLP
                     FPEPTYRSAVHPRTKADVDKLSQALARITEQDPTIHVSRDPDTGETIMTTIGDAQAAI
                     AAARLEKNFGVAVDIAEPRVPYRETITSAAKSEYKHKKQTGGHGQYGHVVIQIDPAQR
                     GAGFTFAEKVVGGNVPKQFIPAVEKGIMETLPTGPLAHSPIVDVQVTLLDGSAHAVDS
                     SEMAFKLAAGQALKQGMLQAHPILLEPVARVIVRVPADRVGDVMSDLNGRRGHVHGVE
                     PAGAFSVVEAEAPLAEVQRYAVDLRALSHGRGRCSVTFDHYAEVPAHVQDQVLKQLTV
                     AEA"
     gene            complement(2220..>2414)
                     /locus_tag="PLX85_06775"
     CDS             complement(2220..>2414)
                     /locus_tag="PLX85_06775"
                     /inference="COORDINATES: protein motif:HMM:NF037628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62911.1"
                     /translation="AVGKDGSHLQFKLKEGAVTWRAIAFGNAANAVPDGECADLVYTF
                     RRDALRGTLQLEVLDLRPAG"
BASE COUNT          345 a          840 c          829 g          400 t
ORIGIN      
        1 ggcggggcag cggtgcggac gtgccgatta cgcttcggcg acggtcagct gcttcagtac
       61 ctggtcctgc acgtgggcgg gcacctcggc gtagtggtcg aaggtgacgc tgcagcgacc
      121 ccgtccgtgg ctgagcgcgc gcaggtccac cgcgtagcgc tgcacctccg cgaggggcgc
      181 ctcggcctcg acgacgctga acgcgccggc gggctcgacg ccatggacgt ggccccggcg
      241 cccgttgagg tcgctcatca cgtcgccgac acggtcggcc ggcacccgga cgatgacgcg
      301 cgcgacgggc tcgagcagga tcggatgcgc ctgcagcatg ccctgcttca atgcctgccc
      361 tgctgccagc ttgaacgcca tctcggacga gtccaccgca tgcgcggagc cgtcgagcag
      421 cgtcacctgc acgtccacga ttggtgaatg cgcgagcggg ccggtgggca aggtctccat
      481 gatccccttc tccaccgcgg ggatgaactg cttcgggacg ttcccgccga cgaccttctc
      541 ggcgaaggtg aagcccgccc cgcgctgcgc cgggtcaatc tggatgacga cgtggccgta
      601 ctgcccgtgc ccgccggtct gcttcttgtg cttgtactcc gacttcgccg cgctcgtgat
      661 cgtctcgcgg taggggacgc gcggttcggc gatgtcgacc gcgacgccaa agttcttctc
      721 gaggcgcgcc gccgcgattg ccgcctgtgc gtcgccaatc gtcgtcatga tcgtttcgcc
      781 cgtgtcgggg tcacggctga cgtggatggt cgggtcctgc tcggtgatgc gcgcgagggc
      841 ctgcgacagc ttgtccacgt ccgcctttgt cctcggatgc accgccgagc ggtacgtcgg
      901 ctcggggaac ggcaggggag gcacgcgagt gcccctgctg gcctcgctgg cgacgaaggt
      961 gtcgccggtc gtggtcacgg tcagcttggc cgcgacgccg atgtcgcccg ccacgagctc
     1021 gggcacgccg atctgttcct tgccacgcat cgcaaagagg tgcccgaggc gttcctgcgt
     1081 tgcgtgctgc acgttgtacg ggttggcgtc cggcttcagc gtcccggaga gcacgcgcat
     1141 gtaggtcagc cggcccacga acgggtccgc ggtcgtcttg aagacgtgcg ccacgagggg
     1201 ccccgagggg tcgacggtca ccgtcgctcc gccctccagc agccgctcgc ggcccacggg
     1261 cgacggcagc agggtcgccg cggcatccat gacgagcttc acgccgaatg cgctcgtccc
     1321 cgagacggga aacacgggga tcacgctgcc gctgcggacc gcctcgggca gcgcggcctg
     1381 caggcgggcg tcgtcgatgg cgttcccttc gaggtagtcg ttgagcagtt cctcgtcagt
     1441 ctccgcgacc gactcaacaa gcatgtcgcg ggcggattcg accgcgcccg cgacatcggc
     1501 tggcacttcg cccgcggcgc cgtccgcgcc acggatcgcc gtgccgctgg cgaggtcgac
     1561 gacgcccgcg aagctgcccg cgacgccgat gggaacggcg aggggcacga cgctcgtgcc
     1621 gaaccggtcg cgcagcgccg ccagcaccgc gtcgaagtct gcgttctcgc ggtcgagcct
     1681 ggtcacgatg ataaagcgcg gccggatgcc cgcctcgtcc gcgtgatccc acgcgacctc
     1741 gctgccgctt tggatgccac cgacggcatc gacggtgatc acagccgcgt ccgcggccgc
     1801 agcggcggcg atcgcctcgc cctcgaagtc ggggtacccc ggcgcgtcga ggacgttgag
     1861 cttcgtccct gcccactcca cgggaatgag tgacgtcgaa atcgagtagt ggtgctggtg
     1921 ttcctgttcg tcgaagtccg aaaccgtgtt gcggtcctcg accttcccca tccgcgaaat
     1981 ggcgcccgat acgaagagca tcgcctcagc gagcgtcgtc ttccccgaac cgccgtgccc
     2041 catgagcacc aggttccgca gttggtccga cgcgtacgct ttcatgtggg cgactcccct
     2101 cgtctgccgc gatggccgcg atggtacaca gtcgtgatag gcgcgacaaa cgcctgacgc
     2161 atacccgggc aggggcggct caacccagtg taggaagctc gcgcaatggc cgtttggccc
     2221 tagccagcag gacgcaggtc gagcacctcg agctggagcg tgccccgcag cgcatcgcgg
     2281 cggaaggtat agacgaggtc ggcgcactcg ccatccggga ccgcgttggc cgcgttgccg
     2341 aatgcgatcg cgcgccacgt aacggcgcct tccttcagct taaactggag gtgcgagccg
     2401 tctttgccga cggc
//
LOCUS       DAORPG010000287         2400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5085_length_2400_cov_3.155224, whole genome shotgun sequence.
ACCESSION   DAORPG010000287 DAORPG010000000
VERSION     DAORPG010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5085_length_2400_cov_3.155224"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /gene="glyA"
                     /locus_tag="PLX85_06780"
     CDS             complement(<1..1081)
                     /gene="glyA"
                     /locus_tag="PLX85_06780"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRC62912.1"
                     /translation="MTTIASRETLAQVDPAIHAAIGREEARQRGTLEMIASENFTSTA
                     VIEAVGSVLTNKYAEGYPGKRYYGGCEFIDEIEQIAIDRAKALFGVEHANVQPHSGAQ
                     ANMGVYMGVIKPGDTVLGMKLDQGGHLTHGSNVNFSGKLYNFASYGVDRETETIDYEQ
                     MAALAREHRPKIIVVGATAYPRTIDFARARAIADEVDAVLMADMAHIAGLVAGGVHPS
                     PAGVAQLITSSTHKTLRGPRGGMILCDEAFARRIDRGVFPGAQGGPLMHVVAGKAVSF
                     LEAATEEFKAYSRQIVENAQALAATLNDAGLRLVSGGTDNHLLLVDVTTKGLDGQRAE
                     DALARCGIIVNKNAIPYDTLPPAVAS"
     gene            complement(1164..2120)
                     /locus_tag="PLX85_06785"
     CDS             complement(1164..2120)
                     /locus_tag="PLX85_06785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HRC62913.1"
                     /translation="MIITVALNPAVDQTVEVDHFAPGDTNRVISIRWDIGGKGINVAR
                     VLKELGYEALATGFAPGPQGRMIEDQLADSGIGCDFVYVPGDTRTNITILERSTHTHT
                     QLALSGPTLPADAPSQLLDRLKRRVRNDTWLALAGSIPPPGDPQVYVDLIAMAAERGG
                     RTALDADGPVVEAVLHSGVRPTLLKLNRLEISRLLHREVQTAEEVLAGARAIHARGVP
                     YVVVTMSKEGAIAVTPDAEYRVHVPPVEIVSAVGAGDAFLSGLLCGLVREGDWTAALA
                     LAAAASTAACLTPGTVLCAGPEVERLRPLVAVERIREHSLAR"
BASE COUNT          343 a          693 c          974 g          390 t
ORIGIN      
        1 cggaggcgac cgcggggggc agggtgtcgt aggggatggc gttcttgttg acgatgatgc
       61 cgcagcgggc gagggcgtct tcggcgcgct ggccgtcgag gcccttcgtg gtgacgtcga
      121 cgagcaagag gtggttgtcg gtgccgccgc tgacgaggcg gagcccggcg tcgttgaggg
      181 tggcggcgag ggcctgagcg ttctcgacga tctggcgcga gtaggccttg aattcctcgg
      241 tagcggcttc gaggaagctg acggccttgc cggcgacgac gtgcatgagc gggccgccct
      301 gtgcgccggg gaagacgccg cggtcgatac ggcgggcgaa ggcctcatcg cagaggatca
      361 tgccgcctcg agggccgcgc agggtcttgt gggtgctcga ggtgatgagc tgggcgacgc
      421 cggcggggga ggggtggacg ccgccggcga cgaggccggc gatgtgggcc atgtcggcca
      481 tgaggacggc gtcgacctcg tcagcgatgg cacgggcgcg ggcgaagtcg atggtgcgcg
      541 ggtaggcggt ggcgccgacg acgatgatct tcggccggtg ttcgcgcgcg agggctgcca
      601 tctgctcgta gtcgatggtc tcggtctcgc ggtcgacgcc gtaggaggcg aagttgtaga
      661 gcttgccgga gaagttgacg ttggagccgt gggtgaggtg gccaccctgg tcgagcttca
      721 tgccgagaac ggtgtcaccc ggcttgatga cacccatgta gacgcccatg ttggcctgcg
      781 cccccgagtg gggctggacg ttggcgtgct cgacgccgaa gagcgccttc gcgcggtcga
      841 tggcgatctg ctcgatctcg tcgatgaact cgcagccacc gtagtagcgc ttgccggggt
      901 agccctccgc gtacttgttg gtgaggacac tgcccaccgc ctcgatgaca gccgtcgagg
      961 tgaagttctc ggaggcgatc atctcgaggg tgccgcgctg gcgagcctcc tcccgaccga
     1021 tggcggcgtg gatggccggg tcgacctgag cgagcgtttc cctgctggcg attgtggtca
     1081 tcgtcgtgtc ccccgctgaa cgtactggca ggcgcatacg ttaccgcagc gcggggttgg
     1141 gcgctggccc ggcgagcggg gcttcagcgg gccaggctgt gctcgcggat gcgttcgacg
     1201 gcaacgagcg ggcggaggcg ttccacctcg ggaccggcgc agaggacggt gccgggggtg
     1261 aggcaggcgg cggtgctggc ggcggccgcg agggcgagag ccgcggtcca gtcgccctcg
     1321 cggacgagtc cgcaaagcag gccgctcagg aaggcgtcgc cggcgccgac ggcggagacg
     1381 atctcgaccg gcgggacgtg gacgcggtac tcggcatcgg gcgtgacggc gatcgcgccc
     1441 tccttgctca tcgtaacgac gacgtagggg acgccgcggg cgtggatcgc gcgcgcgccg
     1501 gcgagcacct cttcggcggt ctggacttcg cggtggagga ggcgggagat ctcgaggcga
     1561 ttgagcttga ggagggtggg gcggactccg gagtggagca ccgcctcgac caccgggcca
     1621 tcggcgtcaa gggcggtgcg cccacctcgc tcggccgcca tcgcgatgag gtccacgtag
     1681 acctgcgggt cgccgggagg cgggatggag ccggcgagcg cgagccaggt gtcgttgcgg
     1741 acgcggcgtt tcagccggtc gagcagctgg gagggcgcat cggcggggag ggtgggaccg
     1801 gagagtgcga gctgcgtgtg ggtgtgcgtc gagcgttcga ggatggtgat gttcgtgcgg
     1861 gtgtcgcccg gcacgtaaac gaagtcgcag ccgatgccgc tgtcggcgag ctggtcctcg
     1921 atcatgcggc cctgcgggcc gggggcgaag cccgtggcca gggcctcgta cccgagttcc
     1981 ttgagcacgc gggcgacgtt gatgcccttg ccaccgatgt cccagcgaat cgagatgaca
     2041 cggttggtgt cgccgggcgc gaagtggtcg acctcgacgg tctgatcgac ggcagggttc
     2101 agggcgacgg tgatgatcac gggttgcgcc tcgaggcacg ggaagtgccg tttgggaaag
     2161 tgtaggaatt cacagaccgg gatgcgaccg cgttgacagg cgggcagggc gcggcccgcg
     2221 cgggggatgg cagaagcaag gggtaaccgc ctgcgagcta gtcttctcgc gcgattgcga
     2281 gcgcgtagac gctgttagct cccgcatgct tgagcgcgcg tgcggcagcc tcgaaggtcg
     2341 cgcccgtggt ggtcacgtca tcgattagca gcacttcacc aacggcgtcg tgcgcctcga
//
LOCUS       DAORPG010000288         2399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5091_length_2399_cov_2.998720, whole genome shotgun sequence.
ACCESSION   DAORPG010000288 DAORPG010000000
VERSION     DAORPG010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5091_length_2399_cov_2.998720"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="PLX85_06790"
     CDS             <1..864
                     /locus_tag="PLX85_06790"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRC62914.1"
                     /translation="PRGRSGAPAPTAVSSVAREAGVETLTPERIRLETTERLRALRPD
                     VGVLAASGHILPTHLLEAFPHGVLNVHASLLPRHRGASPVAAAILAGDEATGASIMLV
                     VRELDAGPVLGTVTTPIGPLDTTGTLTTRVAELGALRMLELLPGWVAGEVDAEPQDES
                     LTSYAPRLTKQDGVLDWTQPAEALARRVRAFQPWPLATTSYRGDTLTVHEAWPLATST
                     EAPTGAVLAGDGSELTPLLPGRRATAVVACGEGSLALLRVQRPGKRALDIEEYLRGDV
                     GFIGTRLGQTA"
     gene            877..1965
                     /gene="rlmN"
                     /locus_tag="PLX85_06795"
     CDS             877..1965
                     /gene="rlmN"
                     /locus_tag="PLX85_06795"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRC62915.1"
                     /translation="MDLVTPATSTRHLLDLSFDDLETLFSEWGEPRYRARQVAHWSLK
                     RGAVSFEEMSDLPAPLRERLATEFTLAAPAVLATTRADDGSTVKALLDLGGGVAVETV
                     RMDYDADAPHERERSTVCVSTQAGCAMGCVFCATGQQGFTRNLTPAEILAQVLHFTRS
                     GRVTNVVFMGMGEPLANTAATIAAVRWLVHPDGLDMRARGITVSTVGLRAGIRRLAAE
                     RVQVGLTISLHAPNDELRRRLIPTAGGTSVDDLVEAAREYISANGRRVTFAYALLDGV
                     NDSEEQARELAARLRGLQAHVNLIPYNPTAGAGLRRPSRERVRAFQRALQGEGINATV
                     RVERGVEIAAACGQLRTDYARRELLTLV"
     gene            complement(1990..>2399)
                     /locus_tag="PLX85_06800"
     CDS             complement(1990..>2399)
                     /locus_tag="PLX85_06800"
                     /inference="COORDINATES: protein motif:HMM:NF026044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62916.1"
                     /translation="HVDFYQVDLARAIQGAVPVVLTGEAPAVHTYKALLLQSTDSVVV
                     EALPAQMPAHIEVSVSGMRTPDAVISVGDLKPPAGVAIVTSPDVIIAQMSRARGGAAA
                     GEVLPEGEQEEPIEGEGESDGEEASAEAEPVAE"
BASE COUNT          356 a          838 c          859 g          346 t
ORIGIN      
        1 ccgcgagggc gctccggcgc gccggcaccg actgcggtgt cgtccgtggc acgcgaggcg
       61 ggcgtggaaa ccctcacgcc cgagcgcatc cggctcgaaa cgactgagcg gctgcgggca
      121 ctccggccag acgtgggcgt gctcgcggca tcggggcaca tcttgccgac gcatctactg
      181 gaggcatttc cgcacggtgt gctcaacgtg catgcctcgt tgctcccgag gcaccgcggc
      241 gcgtcgcctg tggccgcagc gatcctcgcg ggcgacgagg ccacgggcgc ctcgatcatg
      301 ctcgttgtcc gtgagctcga cgccgggccc gtgctcggca cggtaacgac gcccatcggg
      361 ccactcgaca cgacgggcac cttgaccacc cgcgttgcgg aactgggtgc gctgcggatg
      421 ctggagctgt tgcccggctg ggtcgcgggt gaagtcgacg ccgaaccgca ggacgaatcg
      481 ctgacctcgt atgccccgcg gctgaccaag caggacggcg tcctggactg gacgcagccc
      541 gccgaggcgc tcgcgcggcg tgtacgcgcg tttcagccct ggccgctcgc cacgacctcg
      601 taccgaggcg acacactcac cgtgcacgag gcatggccgc tggccacgtc gaccgaggcg
      661 cccactggcg ccgtcctcgc cggggacgga tcggagctca cgccgctgct gccgggcagg
      721 cgggcgactg ccgtggtcgc ctgtggcgag ggcagcctgg ccttgctgcg cgtacagcgg
      781 ccggggaaac gcgcgctgga catcgaggag tacctgcgcg gtgacgtcgg gttcatcgga
      841 acgcggctcg gccagaccgc ctgatagact cagaccgtgg acctcgtcac acccgccacc
      901 tccacccgtc acctcctcga cctgtccttc gacgacctcg agacgttgtt cagcgagtgg
      961 ggggagcccc gctaccgcgc acggcaggtt gcacactggt cgctgaaacg cggggcggtc
     1021 tcgttcgagg agatgagcga cctgcccgcg ccgctgcgtg agcgtctggc taccgagttc
     1081 acactcgctg cccccgcagt cctcgcgacg acgagggcgg acgacggatc gacggtgaag
     1141 gcgttgctcg atctcggcgg cggggtagcc gtcgagacgg tgcgcatgga ctacgacgcc
     1201 gacgcacccc acgagcggga gcgcagcacg gtgtgcgtct cgacgcaggc ggggtgcgcg
     1261 atgggctgcg tgttctgcgc gaccgggcag caggggttca cgcgcaacct gacaccagcc
     1321 gagatcctgg cccaggtgct gcacttcact cggtcggggc gcgtcacgaa cgtcgtgttc
     1381 atgggcatgg gcgagccact ggccaacacg gccgcgacca tcgcggccgt gcgatggctg
     1441 gtgcatccgg atggcctcga catgcgcgcg cggggcatca ccgtctctac ggtcgggctg
     1501 cgcgcgggca tccgccggct ggccgcagaa cgcgtccagg tcggactgac gatctcgctg
     1561 cacgcgccaa acgacgagct acgtcgccgg ctcatcccaa cggccggcgg cacctcggtc
     1621 gatgatcttg tcgaggccgc gcgcgaatac atcagcgcga acggacgccg cgtgacattc
     1681 gcctacgcgc tcctcgacgg ggtgaacgac agcgaggagc aggcgcgtga gctggccgca
     1741 cggctgcggg ggctgcaggc gcatgtgaac ctgatcccgt acaacccgac ggcgggtgcc
     1801 ggcctgcggc gtccctcgcg agagcgggtg cgggcattcc agcgagccct gcaaggcgag
     1861 ggcatcaacg cgacggtacg cgttgagcgc ggcgtcgaga tcgccgcggc gtgcgggcag
     1921 ctcaggaccg actacgccag gcgagagcta ctcaccctcg tctgaggggg tgtgcagcga
     1981 gtgccgagcc tactcggcga cgggctcggc ctccgcagac gcctcttcgc cgtccgactc
     2041 gccttcgccc tcgatcggct cttcctgctc gccctcgggg aggacctcgc cggctgcggc
     2101 gccgccgcgc gcgcggctca tctgagcgat gatcacgtcc gggctggtca cgatcgcgac
     2161 accggcgggc ggcttgaggt caccgacgct gatgaccgcg tcgggggtgc gcatgccgga
     2221 caccgacact tcgatgtggg cgggcatctg cgcgggcagc gcctcgacaa ccaccgaatc
     2281 ggtcgactgc agcagcagcg ccttgtaggt gtgcacggcc ggagcctcgc cggtcagcac
     2341 gaccgggacg gcgccctgga tcgcccgcgc gaggtcgacc tgatagaagt cgacatgct
//
LOCUS       DAORPG010000289         2392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5132_length_2392_cov_3.542148, whole genome shotgun sequence.
ACCESSION   DAORPG010000289 DAORPG010000000
VERSION     DAORPG010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5132_length_2392_cov_3.542148"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..702)
                     /locus_tag="PLX85_06805"
     CDS             complement(85..702)
                     /locus_tag="PLX85_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HRC62917.1"
                     /translation="MARRAERSPAGRRPRSVAAAAGALQVQLRIELLEVEPLVWRRVR
                     LPETLTLERLHGVLLWTMGWSGGHLHEYEIARQRYGTDDPDWPSPEPILDERRYRLGP
                     MLDDGLRRFTYLYDFGDGWEHRVMVEDRLVPPSGRPLIECLAGENACPPEDVGGPPGY
                     EDFLRVIADPNDEEHEHMRSWIGGSFDPTAFNLDTVNRTLATIRI"
     gene            complement(651..2216)
                     /locus_tag="PLX85_06810"
     CDS             complement(651..2216)
                     /locus_tag="PLX85_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HRC62918.1"
                     /translation="MATDYETLLQDVCELRRQLAHKTTLVESLTAEVLRLRRWTFGRS
                     AEKVDLAIAPELPLEGAATASSEETAASTTAPPSSAPPRLLSVDTPRRRGDERRAPRE
                     LPPDLPRVIRIHEPASCQCPDCGSAMRRLGEDASEQLDFVPGYFQVIRHVRPKLACGA
                     CARIVQAPAPSRPIERGLPTAALLAQVIGAKYADHCPLYRQEGIYRRAGLELPRAMLA
                     NWVAESARLLDPLVGVLERYVMGAGKLHADDTPVPVLEPGKGRTRTARLWAYVRDDRP
                     AAGPDPPAVLYRYSPDRKGEHPQAHLARFTGTLQADGYSGFAPLYADGRLWEAACWAH
                     ARRKYYDVYVTDRSPIAAEALRRIGLLYAIEREIRGQPASVRREARQSRAQPVLEDLR
                     AWLTECLRTLSAKAPLAGAIQYTLTRWTALTRYGEDGALEIDNNAAERAIRALVLGRR
                     NYLFAGSDVGGETAARLYSLIGTCRLGGVDPYRYLHHVLERIATHPVNRLEELLPWRV
                     ALSEAPQGAARAA"
     gene            complement(2272..>2392)
                     /locus_tag="PLX85_06815"
     CDS             complement(2272..>2392)
                     /locus_tag="PLX85_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HRC62919.1"
                     /translation="VWPQAASGAVHLTPAQLAMLLEGIDWRRPERTWQPLHVV"
BASE COUNT          354 a          848 c          828 g          362 t
ORIGIN      
        1 actacggagc gctgaagctc gggaggttct acgagagaaa gatgaagatc gaaggtaagc
       61 gtctcgccga gaccgtcttg acgatcagat cctaatcgtg gcgagggttc ggttgacggt
      121 atcgaggttg aacgcggtgg ggtcgaacga gccgccgatc cagctgcgca tgtgctcgtg
      181 ttcttcgtcg ttcggatcgg cgatcacgcg gaggaagtcc tcgtagcccg gcggcccgcc
      241 gacgtcctcc ggcgggcagg cgttctcacc cgccaggcac tcgatcaggg gccgaccgga
      301 tggcgggacg aggcgatctt cgaccatgac gcgatgttcc cagccgtcgc cgaagtcgta
      361 gaggtaagtg aagcgccgca ggccgtcatc gagcatcggg ccgagtcggt aacgtcgctc
      421 gtcgaggatc ggctcgggcg acggccagtc gggatcgtcg gtgccgtagc gctgtcgggc
      481 gatctcgtac tcgtgcaggt gcccgccgct ccagcccatg gtccagagca gcacgccgtg
      541 cagtcgctcg agcgtcagtg tctccggcag gcgcacccgg cgccacacga gcggctcgac
      601 ctccagcagc tcgatgcgca gctgcacctg cagcgcgccg gcagcggcgg ctacgctgcg
      661 cgggcggcgc cctgcggggc ttcgctcagc gcgacgcgcc atggcagcag ttcctcgagg
      721 cggttcaccg gatgcgtcgc gatgcgctcc agcacgtgat gcaggtagcg gtaaggatcg
      781 accccgccga gccggcaggt gccgatcagg ctgtagagcc gcgcagccgt ctcgccgccg
      841 acgtccgagc cggcgaagag gtagttgcga cggccgagca cgagcgcgcg gatcgcgcgc
      901 tcggcggcgt tgttgtcgat ctcgagcgcg ccgtcttcgc cgtagcgcgt cagcgccgtc
      961 caccgcgtca gcgtgtactg gatcgcgccg gcgagcggcg ccttcgccga gagcgtccgc
     1021 aggcactcgg tcagccaggc gcgcagatcc tcgagcaccg gctgcgcccg gctctgacgc
     1081 gcctcgcgcc gtaccgacgc aggctgcccg cggatctcgc gctcgatcgc gtacagcagt
     1141 ccgatgcgcc gcagcgcctc ggcggcgatc ggcgagcgat ccgtcacgta cacgtcgtag
     1201 tacttgcgcc gtgcgtgcgc ccaacaggcc gcttcccaca gccgaccgtc ggcgtacaac
     1261 ggcgcgaagc cgctgtagcc atcggcctgc agcgtcccgg tgaagcgcgc gaggtgcgcc
     1321 tgcggatgtt cgcccttgcg atccggcgag tagcggtaga ggaccgccgg tggatccggt
     1381 cccgccgcgg gccgatcatc gcgcacgtac gcccagagac gcgccgtgcg cgtgcggccc
     1441 ttgcccggtt ccagcaccgg cactggtgtg tcgtcggcgt ggagcttgcc cgcgcccatc
     1501 acgtagcgct cgagcacgcc gaccagcgga tcgagcagcc gcgcgctctc ggcgacccag
     1561 ttcgcgagca ttgcgcgcgg cagctccagc cccgcgcggc ggtagatccc ttcctggcgg
     1621 tacagcgggc agtgatcggc atacttcgca ccgatcacct gcgcgagcag cgccgcagtc
     1681 ggcaggcccc gctcgatcgg tcgtgacggg gctggagcct gcacgatccg cgcacacgcc
     1741 ccgcaggcga gcttcgggcg cacgtgtcgg atcacctgga agtagccggg gacgaagtcg
     1801 agctgctcgg aggcgtcctc gccgagccgg cgcatcgcgc tcccgcagtc cggacactgg
     1861 cagctcgccg gctcatggat ccgaatcacc cgcggcagat cgggcggcag ctcgcgtggt
     1921 gcgcgccgct catcaccccg acgacgcggc gtatcgaccg agagcaggcg cggcggtgcg
     1981 ctcgacggcg gcgctgtcgt ggaggccgcg gtctcttccg agctcgccgt cgcggcgcct
     2041 tcgagcggca gctccggcgc gatcgccaga tccaccttct ccgccgagcg gccaaacgtc
     2101 cagcggcgta gccgcagcac ctcggccgtc agcgactcga cgagcgtcgt cttgtgcgca
     2161 agctgcctgc gcagctcaca cacatcctgc agtaacgtct cgtagtccgt cgccacgctt
     2221 cgatcttagc tgagcacgcg cacgatcgca gtgctctcgc acgatctcgc attacacgac
     2281 gtgcaacggt tgccacgtcc gttcggggcg tcgccagtcg atcccttcga gcagcatcgc
     2341 cagctgcgcc ggcgtcaggt gtaccgcgcc gctcgcggcc tgcggccaca cg
//
LOCUS       DAORPG010000290         2391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5145_length_2391_cov_2.666096, whole genome shotgun sequence.
ACCESSION   DAORPG010000290 DAORPG010000000
VERSION     DAORPG010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5145_length_2391_cov_2.666096"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PLX85_06820"
     CDS             complement(<1..827)
                     /locus_tag="PLX85_06820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HRC62920.1"
                     /translation="MQQRGGISRRSFLGRAAAAGAGLAIANVLAFEPWHLEASDPVQI
                     GDPFKVFPDRGWEKAYRNLFQSDSSYVFTCAPNDTHDCLLRAHVKNGVVVRINPTYGY
                     GQAQDLHGNGPSHRWDPRACQKGLALARKFYGDRRVKGAMVRKGFKEWADAGFPRDAT
                     TGKPQMDMTKRGEDGWLKMSWDEAFALAAKTYVNVATTYSGAKGTKLLEEQGYDHDMI
                     EATHEAGTRTIKMRGGMPLLGVGRIQSFNRFANGMALLDDNVRKVGKSAALGSRAWD"
     gene            1030..2082
                     /locus_tag="PLX85_06825"
     CDS             1030..2082
                     /locus_tag="PLX85_06825"
                     /inference="COORDINATES: protein motif:HMM:NF015181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HRC62921.1"
                     /translation="MAAATPLVRVLGPGRNIGKTWLASHLIEELVARGYRVGAIKSSH
                     HLVPPDREGSDTALFGLAGAQPVVFAAGDGTLTRAEPSRDLGRLVAELPPTLDVVVVE
                     GFKADPLGASIEIVPGIPESAVRLWTMDGVLAYEGGMSDLDRLSDALCCALGISASGD
                     ELVRADIRRAARVHGHRCPGLTLGVRMARYAARYLEVSPEPSPSGLVVEVETARCATD
                     AIAAVTGCTTGNRRLLVNEYGKLAATFSFEGRAVRIAARAGLRELALLDAACEHSAHA
                     QDQAYRTFADETLFDAAPAEPLPTPDHHRGGRGLCERCGEEVAAPALERTASGSFCRP
                     CAVATGANSPRLTAVH"
BASE COUNT          344 a          817 c          816 g          414 t
ORIGIN      
        1 ctgtcccagg cacgagagcc gagcgccgcg ctcttaccca ctttgcgcac attgtcgtcg
       61 agcagcgcca tgccattggc gaagcggttg aagctctgga tgcgcccgac gccgagcagc
      121 ggcatgccac cgcgcatctt gatcgtgcgc gtgccggcct cgtgcgtggc ttcgatcatg
      181 tcgtggtcgt agccctgctc ctcgaggagc ttcgtcccct tggcgccgga gtaggtcgtg
      241 gccacgttga cgtacgtctt ggcggcgagg gcgaatgcct cgtcccagga catcttcagc
      301 cacccgtctt cgccgcgctt ggtcatgtcc atttgcggct tgccggtggt cgcgtctcgg
      361 gggaacccgg cgtcagccca ctccttgaag cccttgcgca ccatcgcgcc tttgacgcga
      421 cggtcgccgt agaacttgcg ggccagcgcg aggcccttct ggcaggctcg cgggtcccag
      481 cggtgcgacg gcccgttgcc gtgcaggtcc tgcgcctggc cgtagccgta cgtcgggttg
      541 atgcgcacga ccacgccgtt cttgacgtga gcacgcagca gacagtcgtg cgtgtcgttc
      601 ggcgcgcagg tgaagacgta gctgctgtcc gactggaaca ggttgcgata cgccttctcc
      661 cagcctcgat ccgggaacac cttgaacggg tcaccgatct gcaccgggtc gctcgcctcg
      721 aggtgccagg gttcaaacgc gaggacgttg gcaatcgcca gtcccgcccc tgccgccgcc
      781 gcccgtccga ggaacgacct tctgctgata cccccgcgct gctgcattct gtgcctccat
      841 ccgacgccgc agcgagttct atgagctttg cgctgtcgat tagcgctcca ctgatagttc
      901 gatgttgcga gcatcctcca cccctacgga cgggacacgg acgaaagtcc ccttcaacgg
      961 gtgaccagtc ccgatggccc gggactcacg cctcagctac caatggaagt gcgcagatgg
     1021 gggcattcga tggcagctgc aacgccgctg gtccgagtcc tgggtcccgg ccgcaacatc
     1081 ggcaagacct ggttggcgtc gcatctcatc gaggagctgg tcgcccgcgg ataccgcgtg
     1141 ggggccatca agagctcaca ccacctcgtg ccgccggacc gcgaaggctc ggacaccgcg
     1201 ctgttcggac tggccggagc gcagcccgtc gtcttcgctg ccggggacgg cacactgacg
     1261 cgagcagagc cgagccgcga cctcggccgg ctcgtcgctg agctcccgcc gacgctcgat
     1321 gtcgtggtgg tggaggggtt caaggccgac ccgctcggcg cgagcatcga gatcgtgccc
     1381 gggatacccg agtctgccgt ccgcctctgg acgatggacg gcgtcctcgc ctacgagggc
     1441 ggaatgagtg acctcgaccg gctcagcgat gccctctgct gcgccctcgg gatttccgcc
     1501 agcggcgacg agctggtgcg tgctgacatc cggcgggcgg cgcgagtgca tggccaccgc
     1561 tgtcctggac ttacgctcgg ggtccgcatg gcccgctacg cggcccggta tctcgaggtc
     1621 tcgccggagc cgtcgccgtc cgggctcgtg gtcgaggtgg agacggcgcg ctgtgcgacc
     1681 gacgcgatcg cggcggtgac cggctgcacg acgggcaatc gacggctgct ggtgaacgag
     1741 tacggcaagc tcgcggcgac cttctccttc gaggggcggg ccgtccgcat cgccgcccgt
     1801 gccgggctgc gcgaactggc gttgctggac gcggcctgcg agcacagcgc gcacgcccag
     1861 gaccaggcct accgcacgtt cgccgacgag acgctgttcg acgccgcgcc ggcggagccg
     1921 ctacccactc ccgaccacca ccgcggcgga cgggggctct gcgaacgctg cggcgaggag
     1981 gtcgctgcgc ctgccctcga acgcacggcg tccgggtcgt tctgccggcc ctgcgcggtg
     2041 gccacaggcg ccaactcccc gaggctgact gccgtccact gagcaacgac cgctgggcat
     2101 atcttgtgga cgagtcgtgc aattacattc gtgatctgct agagtcccgc cggcgatagc
     2161 aattgtgatg accaggcgag gcgccgtcag ccatcacggt gggcgtcgtg cttacacgct
     2221 ctcgagcggc gggagggggg gatggtctgg acacagctcg cgcgagcgtg ccgaggtgcg
     2281 cacgtgacgc gggccgtctt ggtggcggca ttcatcggga tcgtgctctg gctggcaccg
     2341 ggatcgttct ggttcggtcc agcgccgggc tacggacatg gcacggtcga t
//
LOCUS       DAORPG010000291         2389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5149_length_2389_cov_4.052271, whole genome shotgun sequence.
ACCESSION   DAORPG010000291 DAORPG010000000
VERSION     DAORPG010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5149_length_2389_cov_4.052271"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PLX85_06830"
     CDS             complement(<1..508)
                     /locus_tag="PLX85_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC62922.1"
                     /translation="MDWADTPEQAAFRQEVRRFIDEGLPAYYKGVAANGGDLGGEGGW
                     QTDRFLGSPEAKAAALEWEKKVSDRGWVAPHWPKEYGGAGMSSMEQFILKQEMAETGA
                     PEVGGQGVAMLGPTVIAHGTEEQKKRFLPATLACEMVWCQGYSEPGAGSDLASLQTRG
                     VRDGDEWVV"
     gene            677..1090
                     /locus_tag="PLX85_06835"
     CDS             677..1090
                     /locus_tag="PLX85_06835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC62923.1"
                     /translation="MEIRLATSADHETIAALWEESRLPSVDLEEWDSLIASPNAMILL
                     AEHEGALSGAVVASFDGWRAYIYHIAVVPGRRRGGVAVALMQEAEARLRTLGARGILT
                     LVNEANDPGLGLTRSLGYEVQPGELVLAKGSAVQP"
     gene            complement(1165..>2389)
                     /locus_tag="PLX85_06840"
     CDS             complement(1165..>2389)
                     /locus_tag="PLX85_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC62924.1"
                     /translation="LRREKDQAIANQQYEYAAELREREVRLQTKLEELEEEVQTERAS
                     DQPLVTDEDIRDVVSMWTGIPLSRIASEESARLLEMERFLKDHVIGQDEAIGAVAKAV
                     RRARAGLKDPKRPIGTFMFLGPTGVGKTYLAEMLTEFMFGSKDNIVRLDMSEFMEKHT
                     VSRLVGSPPGYVGYDDGGQLTDLVRRRSYCLILLDEIEKAHPDVFNMLLQIFDDGHLS
                     DAKGRKVDFRNTIIIMTSNVGSDLIRRDSRVGFATTQDEAKTAEQQYDRMKQRVTEEM
                     KRVFRPEFLNRIDQSVVFHALGQEDIRKIVSLELRDVRENLKAKGIDFEVTQALLDHL
                     GAEGFDPVFGARPLRRVIQNEVEDRLSDALLEGLYNEGDTIRLDYIDGQVTAERIGTV
                     DPPGSAEPAEPALTT"
BASE COUNT          356 a          836 c          772 g          425 t
ORIGIN      
        1 tgacgaccca ctcgtcgccg tcgcggacgc cgcgcgtctg cagcgaggcg aggtcagaac
       61 ccgcacccgg ctcggagtag ccctggcacc agaccatctc gcacgcgaga gtcgccggga
      121 ggaagcgctt cttctgctcc tcggtgccgt gcgcgatcac cgtcgggccg agcatggcga
      181 caccctggcc gcccacctcg ggcgcgccgg tctcggccat ctcctgcttc aggatgaact
      241 gctccatcga ggacatgccc gcgccgccgt actctttcgg ccaatgcggg gcgacccagc
      301 cacggtcgga gaccttcttc tcccactcga gcgcggcggc cttcgcctcc ggcgagccga
      361 ggaagcggtc ggtctgccag ccgccctcac caccaaggtc gccgccgttt gcggctacac
      421 ccttgtagta ggcgggcagt ccctcatcga tgaatctgcg gacttcctgg cggaatgccg
      481 cctgctccgg cgtgtcggcc cagtccatgc tcacctccct aacgtgcctg agtatccagg
      541 ctcggtttgc gtatgtgtta cggcggattc tgcgccgtcg ggcgtgcctc gtcaacgcct
      601 tccgggccac gattcggcgc tttcctcgca atgccctacg ctgtcgcaca ggaccgctgc
      661 gaggaagggc aacaccatgg agatccggct cgccacatcc gccgaccacg agacgatcgc
      721 ggcgctctgg gaggaatctc ggctcccgag cgtcgacctc gaggaatggg acagcctcat
      781 tgccagtcca aatgcgatga tcctcctcgc ggagcatgag ggagcactct cgggcgcggt
      841 cgtcgcctcc ttcgatgggt ggcgggcgta catctaccac atcgcggtgg tacccggccg
      901 acgccgcggt ggtgtcgccg tcgcgctcat gcaggaggct gaggcacgcc tgcgcaccct
      961 cggggcgcgg ggcatcctca ccctcgtgaa cgaagccaac gacccgggcc tcgggctgac
     1021 gcggtcactc ggctacgagg tgcaacccgg agagctggtg ctggccaagg ggtcggccgt
     1081 ccagccctga cgcgtgccac cccgaggcac aaatgagaag agggccgctt gcgcggccct
     1141 cttctcattc actcgttgga gtgcttaggt cgtcagtgcc ggctcggccg gctccgcgga
     1201 accgggcgga tccaccgtgc cgatgcgctc ggccgtcacc tggccgtcga tgtagtcgag
     1261 gcggatggtg tcgccctcgt tgtacaggcc ctcgaggagc gcgtccgaca gccggtcctc
     1321 aacctcgttc tggatcacgc ggcgcagtgg ccgcgcgccg aacaccgggt cgaagccctc
     1381 ggccccgagg tggtcgagca gcgcctgcgt cacctcgaag tcgatgccct tcgccttcag
     1441 gttctcgcgc acgtcccgca gctcgaggct gacgatcttg cggatgtcct cctgcccgag
     1501 ggcgtggaac acgaccgact ggtcgatacg gttgaggaac tcgggccgga agacccgctt
     1561 catctcttcg gtgacccgct gcttcatccg gtcgtactgc tgctcggccg tcttcgcctc
     1621 gtcctgagtc gtcgcgaagc ccacgcggga gtcgcggcga atcaggtcag acccgacgtt
     1681 cgaggtcatg atgatgatgg tgttgcggaa gtcgaccttc cgccccttgg cgtcggacag
     1741 gtgcccgtcg tcgaagatct gcagcagcat gttgaagacg tccgggtgtg ccttctcgat
     1801 ctcgtcgagc aggatcaggc agtacgagcg ccgccgcacg aggtcggtca gctgcccgcc
     1861 gtcgtcgtag ccgacgtagc cgggcggcga cccgacgagg cgggacaccg tgtgcttctc
     1921 catgaactcg gacatgtcga ggcggacgat gttgtccttc gacccgaaca tgaactcggt
     1981 cagcatctcg gcgaggtagg tcttaccaac accggtgggc ccgaggaaca tgaacgtccc
     2041 gatcggccgc ttcgggtcct tcaggccggc tcgcgcgcgg cgcacggcct tcgcgaccgc
     2101 cccgatcgcc tcgtcctggc caatcacgtg atctttgagg aagcgctcca tctcgagcag
     2161 ccgcgccgac tcctccgacg cgatgcggga gagcgggatg cccgtccaca tggagacgac
     2221 gtcccggatg tcctcgtcgg tgacgagcgg ctggtccgag gcgcgctcag tctgcacctc
     2281 ttcctcgagc tcctcgagct tggtctggag gcgcacctca cgctcgcgca gctcggcggc
     2341 gtactcgtac tgctggttcg cgatcgcctg gtccttctcg cgccgcagc
//
LOCUS       DAORPG010000292         2383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5170_length_2383_cov_3.227234, whole genome shotgun sequence.
ACCESSION   DAORPG010000292 DAORPG010000000
VERSION     DAORPG010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5170_length_2383_cov_3.227234"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1575)
                     /locus_tag="PLX85_06845"
     CDS             complement(<1..1575)
                     /locus_tag="PLX85_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC62925.1"
                     /translation="MEPEPYRESVRMTPRRFALPGFAVLVAAVALLLVSGGPSRSSAV
                     EAQRSGLTWTGCGGSFECATLAVPLDYGQPGGRTIDVALLRLPARKPSERIGALLSNP
                     GGPGGSGIEFMRGWSRILDGDIRDRFDLVSFDPRGVGDSSPLLCHDDARDLIGLEPEP
                     STADEWSEVRRALRAFADLCAERGGAVLPHLGSVDVVRDMDRIREALGEQQITYVGYS
                     YGTVLGALYAELFPQRVRAMVLDGPVDLSLSPDELNVTQAVGFERALSHFVADCRERQ
                     CALARNGRDPGAALQQVVEAARAHPIPSSDADRPARSGEALTAIVASMYRKEFWPSLV
                     QAVEEGLNGNGTWLVQLADRLWGRSGDEYDNSMEMNTAVNCLDYAYSPDPAHYEEASR
                     AAAAVSPTFGASFGTTGLVCAYWHASPQPLQSPDAAEAPPLLVVGTTGDPATPFEWAL
                     ATRRDLVTSTLLTFEGEGHTAYASGNHCVDEVVNAYLLERRLPQDGAICGREESPRAP
                     ATPPAAAFPAKGTLATP"
     gene            1619..>2383
                     /locus_tag="PLX85_06850"
     CDS             1619..>2383
                     /locus_tag="PLX85_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRC62926.1"
                     /translation="MPDTAPTDSSATPPVTVTQVLCAFAYANDLAFGLQLEDTLRSAY
                     VAWRVATELGLSDAARHDAYVTALLKDAGCTSWTTELAAAWHTDEIAARRDFLIFEPS
                     NGMPAFVRWMRRHVGQDEASLAKLRRYLNVLRTSRPFFTEGFATTVTVASRIAGRLGM
                     SPGVQQALLAVFEQWDGSGYPEGRAGAQIPQVARVVLPTFFLVPVHRVSGRAAAIELA
                     TTLRGHAFDPSVADAFLALAMDAGVDHLLRHLADRHL"
BASE COUNT          343 a          914 c          785 g          341 t
ORIGIN      
        1 tggcgttgcc agcgtgccct tcgccgggaa ggccgcagct ggcggcgtcg cgggcgcgcg
       61 cggcgactcc tcgcggccgc aaatggcgcc atcctgcggg aggcgtcgct cgaggagata
      121 cgcgttcacc acctcgtcca cgcagtggtt cccggaggcg tacgccgtgt gcccctcgcc
      181 ctcgaacgtc agcagcgtcg aagtcaccag gtctcgacgc gtggccagcg cccattcaaa
      241 gggcgtggcg gggtcgcccg tcgtgccgac tacgagcagc ggcggcgcct ccgcggcgtc
      301 cggcgactgg agtggctgcg gcgaggcgtg ccagtaggca cagacgaggc cggtcgtacc
      361 gaacgaggcg ccgaacgtcg gcgagacggc cgccgctgcg cgcgaggcct cctcgtagtg
      421 cgccgggtcg ggtgagtacg cgtagtcgag gcagttcacc gccgtgttca tctccatcga
      481 gttgtcgtac tcgtcgccgc tgcgtcccca gaggcggtcg gcgagctgca ccagccacgt
      541 gccgttcccg ttcagccctt cctcgacggc ctgcacgagc gacggccaga actccttgcg
      601 gtacatcgac gccacgatcg ccgtcagcgc ctcgcctgag cgtgcgggcc ggtccgcgtc
      661 gctcgagggg atcggatgcg cacgtgctgc ctcgaccacc tgttgcagcg ccgcccccgg
      721 gtcgcgtccg ttgcgagcca gggcgcactg ccgctcgcgg cagtcggcga cgaagtgcga
      781 caacgctcgc tcgaagccga cggcctgcgt cacgttcagc tcgtcgggcg acagcgacag
      841 gtccaccggc ccgtcgagga ccatcgcgcg cacgcgctgc gggaacagct ccgcgtacag
      901 cgccccgagg acggtcccgt acgagtaccc cacgtacgtg atctgctgtt cgcccagcgc
      961 ctcccggatg cggtccatgt cgcggacgac gtcgacgctg ccgaggtgcg gcaggacggc
     1021 gccaccccgc tcggcacaga ggtccgcgaa cgctcgcagc gcgcgccgca cctcgctcca
     1081 ctcgtcggcc gtcgagggct ccggctcgag tccgatcagg tcccgggcgt cgtcgtggca
     1141 gagcagcggg ctgctgtcgc ccactccccg agggtcgaac gacaccaggt cgaagcggtc
     1201 gcgaatgtct ccatcgagga tgcgcgacca cccccgcatg aactcgatcc ccgagccacc
     1261 cggccccccc gggttcgaca gcagtgcgcc gatgcgttcc gacggcttgc gggccggcaa
     1321 ccggaggagc gcgacgtcga tggtgcgccc gcccggctgc ccgtagtcga gcggcaccgc
     1381 cagcgtggcg cactcgaacg accccccaca cccggtccac gtcagccccg accgctgcgc
     1441 ctcgaccgcc gaggaccgcg acggtccccc gctcacgaga agcaacgcga cagcggcgac
     1501 aaggacggcg aacccgggga gcgcgaagcg acgaggagtc atgcgcaccg attcacgata
     1561 cggctccggc tccacagccg cccctcctat actcggtgcc tgcggtcaag gaggctccat
     1621 gccagacacc gcgcctacag actcgagtgc cactccgcct gtcacggtca cccaggtcct
     1681 ctgcgccttc gcgtacgcga acgacctcgc cttcggcctg cagctcgagg acacactccg
     1741 ttccgcctac gtcgcctggc gcgtcgccac ggagctcggc ctgtccgacg ccgcccgcca
     1801 tgacgcctat gtcaccgcgc tcctcaagga cgcgggctgc acctcgtgga ccaccgagct
     1861 cgccgccgcc tggcacacgg acgagatcgc cgcccgccgc gacttcctca tcttcgaacc
     1921 ctcgaacggc atgccggcct tcgtgcgctg gatgcggcgt cacgtcggcc aggacgaggc
     1981 gagcctcgcg aagctgcgtc gctacctcaa cgtcctccgc acaagccgcc cgttcttcac
     2041 cgagggcttt gccaccacgg tgacagtcgc gagccggatc gccgggcggc tcggcatgtc
     2101 accgggagtg cagcaggcgc tcctcgccgt cttcgagcag tgggacgggt cggggtaccc
     2161 cgagggccgc gcgggcgcgc aaatcccgca ggtcgcgcgc gtcgtactgc cgaccttctt
     2221 cctcgtcccg gtgcaccgcg tcagcggccg ggccgccgcg atcgagctcg cgaccaccct
     2281 gcgaggtcat gccttcgacc cgtcggtcgc cgacgccttc ctcgcgctcg cgatggatgc
     2341 gggcgtcgat catcttctcc gccaccttgc cgatcggcat ctc
//
LOCUS       DAORPG010000293         2376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5206_length_2376_cov_3.256355, whole genome shotgun sequence.
ACCESSION   DAORPG010000293 DAORPG010000000
VERSION     DAORPG010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5206_length_2376_cov_3.256355"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PLX85_06855"
     CDS             complement(<1..582)
                     /locus_tag="PLX85_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62927.1"
                     /translation="MARAKARRSRAVARGAPQFPDLFTVGGRLLRPEVVGTVLVVMAA
                     AVIPFLFPFAGVLSEGRDGMIRALGVHVFTLTLIVAAIGTMLALQYTIWLRRHVRHLF
                     GASMLLVFSAGVLGLWKPATSVGGVSLALHSAGGDFGRFLVGEPVALLVWIALLPVGF
                     TLVWPRTARMIALNTPPVSAAIARATGTGLGLAI"
     gene            complement(639..2288)
                     /locus_tag="PLX85_06860"
     CDS             complement(639..2288)
                     /locus_tag="PLX85_06860"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRC62928.1"
                     /translation="MTALRVIPLGGLGEIGRNMMLYEYGEDMIAVDVGLMFPEEEMLG
                     VDLVIPEITYVMDDPSKLKAVFLTHGHEDHIGALPFLLKEVNVPVFCTRLTRGLVEVK
                     LREHKLHQTAQVNVVEVGQTVNVGAFGVEFFSVSHSIPDAAGLIVHTPLGPVVHTGDF
                     KIDHTPVMDQHTDLQRLAEIGREGALLLAMDSTYVEIEGYTPSEQTVAESLDRAIADA
                     PDRVIVTTFASLISRIQMVIDAAEKHGRRVFVTGRSMVNNVQMARDLGYLRVPAGIMM
                     NAREMTEHPTAKTVIICTGSQGEPTSALTRMANGTHQNVTIQRDDTVILSSNPVPGNE
                     MLVYRNIDNLYRAGAHVIYNRLAPVHVRGHASQEELKIIHRIVRPRHFIGIHGEYRHL
                     VLHRQLAISLGMSEEDAITMTNGDVLEIDEDGARIVDQVSAEYVYVDGLGVGDVDHIV
                     LRDRQHLASDGMVVVVVALNRQLGELLRPPEVMSHGFVGPEEEPEIIEGVQSVVMNVL
                     GGERQHVDWTGIRETLKDDVATYLHQQTHRRPLVLPITVEV"
BASE COUNT          413 a          769 c          800 g          394 t
ORIGIN      
        1 gatcgccagg ccgaggcccg taccggtcgc acgggcgatc gccgctgaga ccgggggcgt
       61 attcaacgcg atcattcgcg cggtccgtgg ccacacgagc gtgaacccga cgggcaggag
      121 ggcgatccag acgaggagtg caaccggctc gccgaccagg aagcggccga agtcgcctcc
      181 cgccgagtgg agtgcgagtg acactccgcc gacggacgtc gccggtttcc acagcccgag
      241 cacaccggcc gagaacacga gcagcatcga ggcgccgaag aggtgccgta cgtgccgccg
      301 cagccagatc gtgtattgca gcgcgagcat cgtgccgatc gcagcgacga tcagcgtcag
      361 cgtgaacaca tgcacgccaa gggcgcgaat catgccgtcg cggccttcgc tgaggacgcc
      421 ggcgaacggg aaaaggaagg ggatcacggc ggcggccatg accaccaaga ccgtaccgac
      481 cacctccgga cggagcaggc ggccgccaac ggtgaagagg tcggggaact ggggcgcccc
      541 gcgcgcaaca gcgcgcgagc ggcgggcttt cgcacgagcc atggctctgg tctcctcgtc
      601 cggctgggcc cgccgcgcag ccggctcgat gtatcgggct acacctccac ggtgatggga
      661 agcaccagtg gacgccgatg cgtctgctgg tgcaggtagg tcgccacgtc gtccttcagt
      721 gtctcacgga tgcccgtcca gtcgacgtgc tggcgctcgc cgccgagcac gttcatcacg
      781 acgctctgca cgccttcgat gatctccggc tcctcctcgg gccccacaaa gccgtgcgac
      841 atcacctcgg gcggtcgcaa cagctcgccg agctgccggt tcagcgcgac gacgacgacg
      901 accatgccgt cgctcgcgag gtgttgtcgg tcccgcagca cgatgtggtc gacatcgccc
      961 acaccgaggc catcgacgta cacgtactcg gcggacacct ggtcgacgat gcgcgcgcca
     1021 tcctcgtcga tctcgaggac gtcgccgttg gtcatcgtga ttgcatcctc ctcggacatc
     1081 ccgagtgaga tcgcaagctg tcgatgcagc accaggtgcc ggtactcgcc gtggatgccg
     1141 atgaagtgtc ggggccgcac gatgcggtgg atgatcttca gttcctcctg ggaggcatgt
     1201 ccgcgcacgt gcacgggagc caggcggttg tagatcacat gcgcgcccgc tcgatagagg
     1261 ttgtcgatgt tgcgatagac cagcatttcg ttgccgggca cgggattcga ggaaaggatg
     1321 acggtgtcgt cacgttggat cgtgacgttc tggtgagtgc cattcgccat gcgggtgagg
     1381 gcggaggttg gttcaccctg cgagcccgtg cagatgatga cggtcttggc cgtcggatgc
     1441 tccgtcatct cgcgggcgtt catcatgatg ccagccggca cgcggaggta cccgaggtca
     1501 cgcgccatct gcacgttgtt caccatcgag cgtccggtga cgaacacccg tcgtccgtgc
     1561 ttctcggcgg catcgatgac catctgaatc cgcgagatca gcgaggcgaa cgtcgtcacg
     1621 atcacgcggt cgggagcgtc cgcgatcgcg cggtcgagtg actcggcgac cgtctgctcc
     1681 gacggcgtgt acccctcgat ctcgacgtag gtcgagtcca tcgcgaggag gagcgcgcct
     1741 tcgcggccga tttcggcgag ccgctgcagg tcggtgtgct gatccatcac tggcgtgtgg
     1801 tcgatcttga agtcgccggt atggacgacc ggcccgagag gcgtatgcac gatgagcccg
     1861 gctgcatcag ggatcgagtg cgagacggag aagaactcga cgccaaacgc gccgacattg
     1921 accgtctggc ccacttcgac cacgttgacc tgcgccgtct ggtgcagttt gtgttcccgc
     1981 agcttcacct cgaccagtcc gcgcgtgagc cgggtgcaga agaccgggac gttcacttcc
     2041 ttgagcagga aaggcagcgc gccgatgtga tcctcgtggc cgtgcgtcag gaagaccgcc
     2101 ttcagcttcg acggatcgtc catgacataa gtgatctcgg ggatcacgag gtcgacgccg
     2161 agcatctctt cctcggggaa catcagaccg acgtcgacgg cgatcatgtc ctcgccgtac
     2221 tcgtagagca tcatgttccg gccgatctcg ccgaggccac ccagcgggat cacccgcagt
     2281 gcggtcacag cagccgcccc tgatccgtgt cggccgtggc ggcgaccggc ccagcgtcga
     2341 gggaagccgg tgcggggcgc gcggctggcc ggccga
//
LOCUS       DAORPG010000294         2369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5221_length_2369_cov_3.643475, whole genome shotgun sequence.
ACCESSION   DAORPG010000294 DAORPG010000000
VERSION     DAORPG010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5221_length_2369_cov_3.643475"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="PLX85_06865"
     CDS             complement(<1..1563)
                     /locus_tag="PLX85_06865"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRC62929.1"
                     /translation="MPFDPRHRSHTLVDGPGRAPARSYFKSIGLTSDDLRKPLVMVAH
                     EWIGTMPCNFSQRALAQQVMAGIRAAGGTPMEVNTISISDGISMGTEGMKASLISREV
                     ITDSIELCGVGYSFDAVVIIVGCDKTIPAASMALLRLNIPGLVLYSGSIAPGRYEGHD
                     ITIQDVFEGVGQHAAGSIGDAQLAAIEDAACPGAGACGAQYTANTMAIVTEFIGLSPF
                     GSASPGATDPRKDDVGRRAGELVMDVLHRGQLPRDIVDRRAIENGIIAAASTGGSTNV
                     VLHLLAIAHEAGIPLDIDDFDKISEQTPLIGDLRPGGRYVALDMDRAGGTPLLAKRLL
                     EAGKLHGDTMTVTGRTIAEECANAAEEPGQDVILPVEKALKATGGLVILKGNLAPAGC
                     VVKVAGHERMQHRGPARVFDREEDAFQAVTDRRINPGDVVVIRYEGPKGGPGMREMLG
                     VTAALVGEGLGESVALLTDGRFSGATRGLMAGHVAPEAFVGGPIAALREGDIVSLDIE
                     ARRLDVELSDDEI"
     gene            1706..2185
                     /locus_tag="PLX85_06870"
     CDS             1706..2185
                     /locus_tag="PLX85_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRC62930.1"
                     /translation="MYKKILLTHDGSDLAGAAVRHAAGIAKAMGAEVVVLQVVDSVSQ
                     LMAQMTTGTIEPLPAGPLTAEIAQESVSGQHDLAEQNLGGVRDALKEQGVEQVSMVVV
                     EGRPEDAIVQAQQELGCDLVVISTHGRTGFRRAMMGSVADHVIRNTPGAPVLVVHLD"
BASE COUNT          357 a          763 c          876 g          373 t
ORIGIN      
        1 gatctcgtcg tcgctcagtt cgacgtcgag gcggcgcgcc tcgatgtcga ggctgacgat
       61 gtcgccctca cgaagcgcgg cgatcgggcc gcccacgaac gcctcggggg cgacgtggcc
      121 ggccatgagg ccccgggtgg ctcccgagaa gcggccgtcg gtgagcagcg cgacgctctc
      181 accgaggccc tcgccgacca gcgcggcggt cacgcccagc atctcgcgca tgccggggcc
      241 gcccttcggg ccctcgtacc gaatgacgac gacatcgccc gggttgatgc ggcggtcggt
      301 gacggcctgg aaggcgtcct cttcgcggtc gaagacacgg gcggggccgc ggtgctgcat
      361 gcgctcgtgg ccggcgacct tcacgacgca gcctgcgggg gccagattgc ccttcaggat
      421 cacgaggccg ccggtcgcct tcagcgcctt ctcgacgggc aggatgacat cctggccggg
      481 ctcctcggcg gcgttggcgc actcctcggc gatcgtgcgg ccggtgacgg tcatcgtgtc
      541 gccgtgcaac ttgcccgcct cgagcagccg cttcgcgagc agtggcgtgc cgccagcgcg
      601 gtccatgtcg agggcgacat aacggccgcc gggccgcagg tcaccgatca gcggcgtctg
      661 ctcgctgatc ttgtcgaagt catcgatgtc gagcgggatg cctgcctcgt gcgcgatcgc
      721 gagcaggtgc agcacgacgt tggtcgaacc accggtcgag gctgccgcga tgatgccgtt
      781 ctcgatcgcc ctgcggtcca cgatgtcgcg cgggagctga cctcgatgca ggacgtccat
      841 gaccagttcg ccggcgcgcc ggccgacgtc atccttgcgc gggtcggtcg cgccgggcga
      901 ggcggagccg aagggcgaca ggccgatgaa ctcggtgacg atggccatcg tattggcggt
      961 gtactgggcg ccgcaggcgc cggctccggg gcaggccgcg tcctcgatgg cggccagctg
     1021 agcatcgccg atcgagccgg ctgcgtgctg gccgacgccc tcgaacacgt cctggatcgt
     1081 gatgtcgtgg ccctcgtagc gtcccggcgc gatggagccg ctgtacagca cgaggcccgg
     1141 gatgttgagc cggagcagcg ccatgctcgc ggccgggatg gtcttgtcgc agccgacaat
     1201 gatgacgacg gcatcgaagg aatacccgac gccgcacagc tcgattgagt cggtgatcac
     1261 ctcgcggctg atgagcgagg ctttcatgcc ctcggtgccc atcgaaatgc cgtcgctgat
     1321 ggagatggtg ttgacctcca tcggcgtccc gcccgcggcg cggatgcccg ccatcacctg
     1381 ctgcgcgagg gcgcgctggc tgaagttgca cggcatggtg ccgatccact cgtgggcgac
     1441 catgacgagc ggcttgcgga ggtcgtcgct ggtgaggccg atcgacttga aataggagcg
     1501 agcgggggcg cgacccgggc cgtcgacgag cgtgtgactg cggtggcggg ggtcgaaggg
     1561 catcgagcgg ctcctctgcg caagacgggc gattcgcgag attgtaccaa gggtggcggt
     1621 tcgctcatcc tcgccggcgg gcctcgtgct caaaacgcag acgtgtatga tgcaccgggc
     1681 gaattcatcc cgatggaggc tgtttgtgta caagaaaatc ctgctgaccc atgacggttc
     1741 tgacctggca ggggcggcgg tgcggcacgc ggccgggatc gcgaaggcga tgggcgccga
     1801 agtcgtggtg ctgcaggtcg tggactcggt ttcgcaactg atggcgcaga tgacgaccgg
     1861 cacgatcgag cccctgccgg ccgggccact caccgccgag atcgcacagg agtcggtcag
     1921 cggccagcat gacctcgccg aacagaacct tggcggcgtg cgcgacgcgc tcaaggaaca
     1981 gggcgtcgag caggtgagca tggtcgtcgt cgaggggcgg cctgaggacg cgatcgtgca
     2041 ggcgcagcag gaactgggct gcgacctcgt cgtcatctcg acgcacgggc ggacggggtt
     2101 ccgtcgggcg atgatgggct cggtggccga ccacgtgatt cggaacaccc ccggtgctcc
     2161 ggtgctcgtc gtccacctcg actaggacgc tcggctcaag gcaactcaga cgcgaggccg
     2221 cggcgaaagc cgcggcctac gtcgtggcgt cgtcgggccc ggggatcggg acttccttgg
     2281 agtggccgtt ggagccaggg caaaagtggg cctctccggc ctgttgaatc gggcgaccgc
     2341 agagcgggca gagcggccgg ccggccgcc
//
LOCUS       DAORPG010000295         2369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5222_length_2369_cov_3.585134, whole genome shotgun sequence.
ACCESSION   DAORPG010000295 DAORPG010000000
VERSION     DAORPG010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5222_length_2369_cov_3.585134"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PLX85_06875"
     CDS             complement(<1..466)
                     /locus_tag="PLX85_06875"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC62931.1"
                     /translation="MTAEFRRATLDDAGAISEIIGEVVVEPDPVGLDGPMSPDEVSQW
                     IDRQGKYGAMFVAEEEGAILGFASVDFDSSRAEECTIGAWIRPSARRRGLASILFEEG
                     LGFAGEQGYKRVRGRLPEANEAALSFLSTIGALVPIINPGAQYDMPIYEVRRD"
     gene            complement(483..1586)
                     /gene="carA"
                     /locus_tag="PLX85_06880"
     CDS             complement(483..1586)
                     /gene="carA"
                     /locus_tag="PLX85_06880"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRC62932.1"
                     /translation="MSRRPPARLVLADGRAFEGEGIGAPGRATGEVVFNTSMMGYQEA
                     LSDPSYGGQILTMTYPLQGNYGFNASDDESARIQVRGFVVRELTDLPSHWHSEGTLDT
                     HLTRAGVPGIAGIDTRALTRHLRSHGVLMGTITRDETAAEALERLRREPVYGIEDYVA
                     EVSTDVAYDFDPGDLGGSDGPRPHVVVLDLGVKRSIMRNLAKRGCDVTAVPHRTSAED
                     ILALRPDGVVMSPGPGDPMLLDHVVTTARGLIGRVPLMGICLGHQVVGRVFGASTYKL
                     KFGHRGANHPVRDEVTGRVYITAQNHGYAVDGDHLDAAVRASQIHLNDGTVEGLEHRE
                     LPLFTIQYHSEASPGPLDTEFLFDRFLATVTDR"
     gene            complement(1583..>2369)
                     /locus_tag="PLX85_06885"
     CDS             complement(1583..>2369)
                     /locus_tag="PLX85_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRC62933.1"
                     /translation="QLVRDGHMHEGWVATRLGLRGRPASAEETLVARDIALAELAGAH
                     LHIQHVSTAGSVALIRAARARGAHVTAEVTPHHLTLTHEEVAFGGGDYRQIAYNANAK
                     VNPPLREQADVEACIAALRDGVIDAVATDHAPHALSDKDIEFNLAAAGISGLETAFGL
                     LMSLVHAGHLDLATLVERLTAGPARAWGLERRTGLDGIGTLAPGALGDLVVLDPNAEW
                     TVDPREFASTGRNTPLAGRQLKGRVVATVYGGRVVHALEGVAI"
BASE COUNT          343 a          851 c          792 g          383 t
ORIGIN      
        1 cgtcacggcg cacctcgtag atgggcatgt cgtattgcgc gcccgggttg atgatcggga
       61 ccagcgcgcc aatcgtggag aggaaggaca gggccgcctc gttcgcctcg ggcaggcggc
      121 cgcgcacgcg cttatacccc tgttcccccg cgaagccgag gccctcctcg aacaggatcg
      181 aggcgagccc gcgccggcgc gcgcttggcc ggatccaggc cccgatggtg cactcctcgg
      241 cgcggctgga gtcgaagtcc acggacgcga aaccgaggat cgcgccctct tcttccgcga
      301 cgaacatcgc gccgtacttg ccctgccggt cgatccactg cgagacctcg tccgggctca
      361 tcggcccatc gaggccgacc ggatctggct cgacgacgac ctcgccgatg atctccgaga
      421 tcgccccggc gtcgtcgaga gtggcccgac ggaactctgc ggtcatcctt ctccgcccct
      481 cgttacctgt ccgtgacggt agctaaaaac cggtcgaaga gaaactccgt gtccagcgga
      541 cccggcgatg cctccgagtg gtactgaatg gtaaacagcg gcagctcgcg gtgctccaac
      601 ccctcgactg tcccgtcgtt caggtgaatc tgcgaggcgc gcacggccgc gtcgaggtgg
      661 tcgccgtcga cggcgtagcc gtggttctgc gcggtgatgt acacgcgccc ggtcacctcg
      721 tcgcgcacgg ggtggttggc cccgcgatgc ccgaacttga gcttgtaggt cgacgcgccg
      781 aacacacgcc ccacgacctg gtgaccgagg cagatgccca tcagtgggac acgcccgatg
      841 agccctcggg ccgtcgtcac cacgtggtcg agaagcatcg ggtcaccggg ccccggcgac
      901 atcacaacgc cgtccggccg cagcgcgagg atgtcctccg cggaggtccg atgcgggacc
      961 gccgtcacgt cgcagccgcg cttcgcgagg ttgcgcatga tgctgcgctt cacccccagg
     1021 tcgagcacga cgacatgcgg tcgcggcccg tcgctgcccc cgaggtcgcc gggatcgaag
     1081 tcgtaggcga catccgtaga cacttcggcg acataatcct cgatgccgta cacgggttct
     1141 cgtcgcagcc gctcgagggc ctcggcagcc gtctcgtcgc gcgtaatcgt ccccatgagg
     1201 acgccgtgcg agcgcaggtg ccgggtgagc gcgcgcgtgt cgatgccagc gatgccgggc
     1261 actcccgccc gggtgagatg cgtgtcgagg gtgccctcgg aatgccagtg cgaagggagg
     1321 tcggtcagct cgcgtacgac gaagccgcgc acctggatgc gcgcgctctc gtcgtcggag
     1381 gcgttgaacc cgtagttccc ctgcagggga taggtcatcg tgaggatctg gccgccgtat
     1441 gaggggtcgg acagcgcctc ctgatacccc atcatcgagg tgttgaacac gacctcgccg
     1501 gtcgcgcgcc cgggcgcgcc gatcccctcg ccttcgaacg cgcgcccgtc cgccaggacc
     1561 agccgcgcag gcggccgtcg actcatatcg cgacaccttc cagtgcgtgc actacacgcc
     1621 cgccgtacac cgtcgccaca acgcgcccct tcagctgtcg gcccgccagc ggcgtgttcc
     1681 ggcccgtcga tgcgaactcg cgcggatcca ccgtccattc agcgttcggg tcgagcacca
     1741 cgaggtcccc cagcgccccc ggcgctagcg tcccgatgcc gtcgaggccc gtgcggcgtt
     1801 cgaggcccca cgcccgcgct ggcccggccg tgagccgttc gacgagcgtc gcgaggtcga
     1861 ggtggcccgc gtgtacgagc gacatcagca gcccgaatgc tgtctcgagg ccgctgatcc
     1921 ccgcggccgc aaggttgaac tcgatgtcct tgtcgctcag cgcgtgcggt gcgtggtccg
     1981 tcgctacggc atcgatgacg ccgtcgcgca gcgcggcgat gcacgcctcc acgtcggcct
     2041 gctcacgtag tggcgggttc actttcgcgt tcgcgttgta ggcgatttgc cggtaatcgc
     2101 cgccgccgaa cgcgacctct tcgtgtgtga gcgtcaggtg gtgcggcgtg acctcggcgg
     2161 tcacatgggc gcctcgggcg cgggccgccc ggatgagcgc caccgagcct gccgtcgaca
     2221 cgtgctggat gtgcagatgt gcgcccgcga gctctgcgag cgcgatgtcc cgcgccacga
     2281 gcgtctcctc ggcgctcgct ggccgacccc tcagtcccag gcgggtcgcg acccagccct
     2341 cgtgcatgtg tccgtcgcgg accagctgc
//
LOCUS       DAORPG010000296         2368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5227_length_2368_cov_3.484652, whole genome shotgun sequence.
ACCESSION   DAORPG010000296 DAORPG010000000
VERSION     DAORPG010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5227_length_2368_cov_3.484652"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PLX85_06890"
     CDS             <1..611
                     /locus_tag="PLX85_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017241863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRC62934.1"
                     /translation="VVGRSVDGFTVGFPTEVALDGRTAMVAYGMNGEALPAAHGFPAR
                     LVVPGLYGYVSATKWLSEIELTTLEAFDAYWVPRGWSKQAPIKTQSRIDTPRKNATAG
                     RVPVAGVAWGGIRGIKQVDVRITRAGDSSGTWHRARLGEALSSSSWRQWVVEDWTPEP
                     GEYRLEVQATDGEGTPQTEARHEPAPNGATGLHMVTVRVRAG"
     gene            complement(620..1372)
                     /locus_tag="PLX85_06895"
     CDS             complement(620..1372)
                     /locus_tag="PLX85_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC62935.1"
                     /translation="MRSLGKLTWVELKLFVRDPFTVLFTFAYPFFVLFILAGVFGNTL
                     ETPQDVDTWRGVGPTDYYVPAYVGVVLASIGFLALPLRLAGYRERGVLRRFRASSLSV
                     WWILGSQMLVAAVMAVIGAVSVTVAARIVYGTFWPRDLVLTVLAFLLGVVAFGSIGVF
                     LGAVLPNSRAAQSAGLILFFATFMISGAGPPPEALGANLEAVSRVLPLTHVVRVIQDP
                     WLGYGWSPESSLIVAGMAVASAALSVRLFRWE"
     gene            complement(1369..2265)
                     /locus_tag="PLX85_06900"
     CDS             complement(1369..2265)
                     /locus_tag="PLX85_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007025668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC62936.1"
                     /translation="MPDVIEVEGLRKTYGATVAVDDVSLEVQQGEIFGLVGPNGAGKT
                     TAVECLQGLRHPDAGLVRVLGADPRDPALRGRIGSQLQSSALPDRIRVWEAIDLFASF
                     GARSRDWRQLLEEWGLADRANAYFSALSGGQRQRLFIALALVTAPEVVFLDEMTAGLD
                     PSARRLAWHLVERVRDSGVTVVIVTHFMEEAEQLCDRIAVIDRGRVSALGSPQQLMAQ
                     YAPDIRVSFTIPAADLDWLRATPDVASVAVNGHRVEVRGNNALIVRVAAALADHGLAP
                     ADFRVHQPTLEDVFLRITGAPE"
BASE COUNT          405 a          755 c          912 g          296 t
ORIGIN      
        1 aggtcgtcgg gcggtcggta gacgggttca cggtcgggtt tccgacggag gtcgcgctcg
       61 acgggcggac ggcgatggtg gcgtacggga tgaacggcga ggcgctgccg gcggcgcatg
      121 gcttccccgc gcgcctcgtg gtgccggggc tgtatggcta tgtgtccgcg acgaagtggc
      181 tcagcgagat cgagctgacg acgctcgagg cgttcgacgc gtactgggtg ccgcgggggt
      241 ggtcgaagca ggcgccgatc aagacgcagt cgcgcatcga cacgcctcgc aagaacgcga
      301 cggcggggcg ggtgccggtt gccggggtcg cctggggcgg catccgaggc atcaagcagg
      361 tcgacgtgcg gatcacacgc gcgggagact cgagcgggac gtggcatcgc gcgcgcctcg
      421 gtgaggcgct gtcatcgagc agctggcggc agtgggtggt cgaggactgg acgccggagc
      481 cgggcgagta tcgcctcgag gtgcaggcca ccgacggcga ggggacgccg cagacggagg
      541 cgcggcacga gccggcgccg aacggggcga cgggcctgca catggtcaca gtgcgcgtgc
      601 gagccgggtg acctcggggc tactcccagc ggaacagacg gacgctgagt gcggcggagg
      661 ctacggccat cccggcaacg atgagtgacg actcgggtga ccagccgtag ccgagccacg
      721 ggtcctggat cacgcggacg acatgggtga gcggcagcac gcggctaacc gcctcgaggt
      781 tcgcgccgag cgcctcaggg ggcggtccgg cgcccgagat catgaaggtc gcgaagaaga
      841 ggatgaggcc cgccgactgg gcggcgcggg agtttggcag cacggcgccc agaaacacgc
      901 cgatgctgcc gaaggcaacc acgccgagca ggaaggcgag gaccgtcagc accaggtcgc
      961 gcggccagaa cgtgccgtag acgatgcgcg cggcgacggt cacggagacc gcgccgatca
     1021 ctgccatcac cgcggccacc agcatctgcg agccgaggat ccaccagacc gagagcgatg
     1081 aggcgcggaa ccggcgcagg acgcctcgtt cgcggtagcc ggcgaggcgc agcgggagcg
     1141 cgaggaaccc gatcgaggcg agcacgacgc cgacgtaggc gggcacgtag taatcggtgg
     1201 ggccgacgcc gcgccaggta tcgacgtcct gtggcgtctc cagcgtgttg ccgaacacgc
     1261 ccgcgaggat gaacaggacg aagaacgggt aggcgaacgt gaagagcacc gtgaaggggt
     1321 cgcgcacgaa cagcttcaac tcgacccagg tcagcttgcc gaggctcctc attccggcgc
     1381 gcccgtgatg cgcaggaaga catcctcgag cgtgggctga tgcacgcgga agtccgcagg
     1441 cgccaggccg tggtcggcca gtgccgcggc cacgcgcacg atgagcgcgt tgttgcctct
     1501 gacctcgacg cggtggccgt tgaccgcgac cgaggcgacg tctggcgtgg cccgcagcca
     1561 gtcaaggtcc gcggcgggga tggtgaacga aacccgaatg tcgggcgcgt actgcgccat
     1621 cagctgctgc gggctgccga gagccgacac gcggcctcgg tcgatgacgg cgatgcggtc
     1681 gcagagctgc tcggcctcct ccatgaagtg ggtcacgatg acaacggtca cgcccgagtc
     1741 gcggacgcgc tcgacgaggt gccacgccag tcggcgggcg gaggggtcga ggcccgcggt
     1801 catctcatcg aggaagacga cctcgggcgc ggtgaccagg gcaagggcga tgaacagccg
     1861 ctggcgctgt ccgcctgaaa gcgcggagaa gtaggcgttc gcgcggtcgg cgagtcccca
     1921 ctcctcgagc aactgccgcc agtcgcgcga acgggcgccg aaggaggcga agaggtcgat
     1981 cgcctcccag acccggatgc ggtcgggtag cgccgaggac tgcagctgcg agccgatgcg
     2041 ccctcggagg gcggggtcac gggggtcggc gcccagcact cgcacgagac cggcgtccgg
     2101 gtgccggagt ccctggaggc actcgacggc cgtggtcttg cccgcgccgt tcgggccgac
     2161 gagtccgaaa atctcgcctt gctggacctc gagggagaca tcgtcgacgg cgacggtggc
     2221 gccgtaggtc ttgcgcaggc cttccacctc gatgacgtcg ggcatgcgcg gctccgcttc
     2281 acgctgtcgc cggcgacaac cttacacgct tcggcacgcc cccacgaacg cttcgaacag
     2341 ggcccgtgag ggctcgccgt ccatctcc
//
LOCUS       DAORPG010000297         2362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5254_length_2362_cov_3.661465, whole genome shotgun sequence.
ACCESSION   DAORPG010000297 DAORPG010000000
VERSION     DAORPG010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5254_length_2362_cov_3.661465"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..1491
                     /locus_tag="PLX85_06905"
     CDS             226..1491
                     /locus_tag="PLX85_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRC62937.1"
                     /translation="MGQNGLRNVLLAVVSVLIASMLFAVGFLTRVVVEPDHNDSTPAS
                     TSSAKTLETGDVDPSVLQDIIAILQQDFVDQSRVADPNLLYQGAIQGIFTQLNDPHST
                     YIDPKTYALSKDDFSGAFQGIGATVAKEGDYVVIVRPLADTPAARAGIQGGDRVLAVN
                     GEDATGWSVEKAVLKIRGPKGSTVELKLQHADGSQQTYKLTRDDVLVASVDTRPPGGT
                     LKDASGAEAGDIAYIRISSFTSRTPNELQTEIRNALNRNVKGFIIDVRGNPGGLLNET
                     LQIADMFLDKGAMLTQVDKDGKEQRFDARAGTLTNLPIAIVQDEFSASGSEVLAAALQ
                     ENGRATVVGSRSFGKGTVNHVRELKNGGAVYVSIARWLTPNRNQIEGAGVNPNILVTL
                     TPDDIDQQRDIAVFRAIDALRNPTPQATR"
     gene            1557..2027
                     /gene="smpB"
                     /locus_tag="PLX85_06910"
     CDS             1557..2027
                     /gene="smpB"
                     /locus_tag="PLX85_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRC62938.1"
                     /translation="MPKREPRGTTVAANRRARFEYDVLDELEAGISLVGSEIKSIREG
                     KANIAEGYARFRDGDLWLYNVHIAPYLPARENHEPLRPRRLLLHRRELDRLEQEMREQ
                     PRTTIVPLRLYLHEGLAKVELGLVRGRKSYDKRQAIAKRESERSMQRALRHAQR"
     gene            2068..>2362
                     /gene="ssrA"
                     /locus_tag="PLX85_06915"
     tmRNA           2068..>2362
                     /gene="ssrA"
                     /locus_tag="PLX85_06915"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          460 a          906 c          654 g          342 t
ORIGIN      
        1 gcctcacgat gctgcgcacg gctctgctgc ggcgcgcccc cctggacccg ccgaatcacc
       61 atcccttggc taacattcaa tctagcgttg gctcatcagt acataaccga gatagtgcgc
      121 aatgtatcat gtgctgctag tgtcctctcc caacgccctc cgaaagcggt atagactcgt
      181 ctgaccgcac cccaccagca cgcgcgctca ccgaaggaga cccccatggg ccaaaacggc
      241 ctccgtaatg tgctgctggc ggtcgtctcc gtcctcatcg cctcgatgct cttcgccgtc
      301 ggcttcctca cccgcgtcgt cgtcgagccc gaccacaacg actccacgcc ggcctccacc
      361 tcgagcgcaa agacgctcga gactggcgac gtcgatccca gcgtccttca ggacatcatc
      421 gccatcctcc agcaggactt cgtcgaccaa tcccgagtcg ccgaccccaa cctgctctac
      481 cagggcgcca tccaggggat cttcacccag ctcaacgacc cccactccac gtacattgac
      541 ccgaagacct acgccctctc caaggacgac ttctccggag ccttccaggg catcggcgcc
      601 accgttgcca aggaaggcga ttatgtcgtc atcgttcggc cgctcgccga cacccctgct
      661 gcccgcgccg gcatccaggg cggcgaccgc gtcctcgccg tgaatggcga ggacgccacc
      721 ggctggagcg tcgagaaggc cgtcctcaag atccgtggcc ccaagggctc cacggtcgag
      781 ctcaagctcc agcacgccga cggctcgcag cagacctaca agctgactcg tgacgatgtc
      841 ctcgtcgcct ccgtcgatac ccgcccgccc ggcggcacgc tcaaggacgc ctccggcgcc
      901 gaggccggcg atatcgccta catccgcatc agctcgttca cctcgcgcac ccccaacgag
      961 ctccagaccg agatccgcaa cgcgctcaac cgcaacgtga agggcttcat catcgacgtc
     1021 cgagggaacc ccggtgggct cctcaacgag accctccaga tcgccgacat gttcctcgac
     1081 aagggcgcga tgctcaccca ggtcgacaag gacgggaagg agcagcgctt cgacgcccgc
     1141 gccggcacgc tgaccaacct gcccatcgcc atcgtgcagg acgaattctc cgcctccggc
     1201 tccgaggtcc tcgccgccgc cctccaggag aacggccgcg ccaccgtcgt cggctcccgc
     1261 tccttcggca agggcacggt caaccacgtc cgcgaactca aaaacggcgg cgccgtctac
     1321 gtctcgatcg cgcgctggct cacccccaac cgcaaccaga tcgaaggcgc cggcgtgaac
     1381 cccaacatcc tggtcaccct cacgcccgac gacatcgacc agcagcgcga catcgcggtc
     1441 ttccgcgcta tcgacgcgct gcgcaacccc accccgcagg ctactcgata attatgtaat
     1501 gtagagggcg ggctccccct gcccgcccca ccgcctgccc gaccgaaagg acgcccgtgc
     1561 ccaagcgcga gccccgaggc accaccgtcg ccgccaaccg ccgcgcacgg ttcgaatacg
     1621 acgtcctcga cgagctcgag gccggcatct ccctcgtcgg ctccgagatc aagtcgatcc
     1681 gcgagggcaa ggccaacatc gccgagggct acgcccgatt ccgagacggc gacctctggc
     1741 tgtataatgt gcacatcgcc ccctacctcc cggcacgcga gaaccacgag ccgctgaggc
     1801 ctcggcgtct gctcctccat cgacgcgaac tcgaccggct ggagcaagaa atgcgggaac
     1861 agccgcggac gaccatcgtc ccccttcggc tctacctgca tgaaggcctc gccaaagtgg
     1921 aactcggcct ggtccgaggg cgaaagagct acgacaagcg ccaggccatc gccaagcgcg
     1981 agtccgaacg ctcgatgcag cgtgcgctgc gccacgccca gcgctgacaa cgtgggcgtt
     2041 gagcagtgct cgaggcaaca ccaacctggg gacgaaagga ctcgacaggg actgacggac
     2101 gcggattgcg ggtcgaggtg ccatcaccct cgctaatcag gtggcaaaaa gtaactggcg
     2161 aaaacaagct cgccctcgct gcctaactaa gcagcgacgt ctcatccggc ctcagcccga
     2221 cgggccggga cgaggcgacg actagccggg ccgctctgtc gaggtgcgcc tcaggcctcg
     2281 acggctaaga ccactgagac tggctgcgtg gcccctggcc agtggaggac gcgcagcgag
     2341 accaaccgct ggctacaccc ct
//
LOCUS       DAORPG010000298         2359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5267_length_2359_cov_4.279514, whole genome shotgun sequence.
ACCESSION   DAORPG010000298 DAORPG010000000
VERSION     DAORPG010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5267_length_2359_cov_4.279514"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="PLX85_06920"
     CDS             <1..1098
                     /locus_tag="PLX85_06920"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HRC62939.1"
                     /translation="RLRAAAHGFATASPRRFLDVVRSSSGLQAQDATAGTLSARPRTR
                     GLTEADVRRARFEERSVVRTWAMRGTLHFLPAKDLGWLLPLLGPNAIARDRTRLMQLG
                     LDADAVERGVTVIRDALASHGPLTRDSVREALAADGLPAAGQATQHLLYVAALRGFIC
                     CSNDRGSGTHEFVLLSDWVELGPPLPRATALAELARRYLHAAGVATVEDFAAWSGLPL
                     RDAREGWDAIREERAEVELAREQAWALSSRLPELLAAREYAPIVRLLPRFDSYLLSYR
                     GRDTALDERHARHVLPGGGILNATVLVDGRLVGVWQLHRDKKRATMTVAPFEPLPPEI
                     YAAIEVETDDLGRFLDMPVAVTFGAPLSSGS"
     gene            complement(1095..1553)
                     /locus_tag="PLX85_06925"
     CDS             complement(1095..1553)
                     /locus_tag="PLX85_06925"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRC62940.1"
                     /translation="MTTGTDPALREHYCFGCGRHNPIGFHLVFEREGDAVVARYDPRP
                     EDQGFPEVMHGGLLSLLLDEAMGWAMYADGVFAVTAKMEVRFRRAVGLDAPVTARARI
                     MRSRGRRIEVEASLTNEAGEVLVESSALFMRMSPEDEARALASFRASAGG"
     gene            complement(1550..1945)
                     /locus_tag="PLX85_06930"
     CDS             complement(1550..1945)
                     /locus_tag="PLX85_06930"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRC62941.1"
                     /translation="MSTPPEELRLVRLIDQGRVDAYADAARDHNPIHVDEVFAAASPF
                     GTRIAHGMLVLALVSELMSAAFGEAWARSGALKVRWRAPAVPPVNVSARALLRSSSDG
                     VATYDVSCEAEDGTVLLAGTASVSRGTSA"
     gene            complement(1942..2346)
                     /locus_tag="PLX85_06935"
     CDS             complement(1942..2346)
                     /locus_tag="PLX85_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRC62942.1"
                     /translation="MSIDFHALRRGDRLPPIAFTVTLEDVRAYLAATGEAAGTWSAHV
                     PPLALGAYILAGLMEVMPLPPGALHAGQEFEFLDTVAQGEPLEAELTVAQQSERQGSN
                     IVVFASELKAGERVVLRGRTTVMAPVATSEAG"
BASE COUNT          361 a          889 c          781 g          328 t
ORIGIN      
        1 cggctgcgcg ccgccgcaca cgggttcgcc acggccagcc cgcgccgctt cctcgacgtc
       61 gtccgctcca gcagcggcct gcaggcgcag gacgcgacgg ctggaacgct ttcggcgcgg
      121 cctcggacgc gaggcctgac cgaggcggat gtgcgtcggg cccgcttcga ggaacgcagc
      181 gtggtccgca cctgggctat gcgcggcaca ctgcatttcc tccccgccaa ggacctcggg
      241 tggctgctgc cgctgctcgg tccaaacgcc atagcgcgcg accgcacacg cctcatgcag
      301 ctcggattag atgccgatgc ggtcgaacga ggcgtgacgg tgatccggga tgccctggcc
      361 agccacggcc cactcacgcg cgactcggtc cgcgaggccc tcgcagcgga cgggctgccc
      421 gccgccggcc aggcgacgca gcacctcctc tacgtcgcgg cactgcgggg cttcatctgc
      481 tgcagcaacg accggggcag cggcacgcac gagttcgtcc tgctctccga ctgggtcgag
      541 ctcggtccgc ccctgccacg cgcgacggcg ctcgccgaac tcgcgaggcg ctacctccat
      601 gccgccggcg tcgccaccgt cgaggacttc gccgcgtggt ccgggctgcc gctacgcgac
      661 gcccgcgagg ggtgggatgc gatccgcgaa gaacgagccg aggtagaact cgctcgagaa
      721 caggcgtggg cgctgtcgag ccgtctcccg gaactcctgg cagcgcgcga atatgcaccc
      781 atcgtgcgcc tgctcccacg gtttgacagc tacctgctgt cgtaccgtgg ccgcgacacc
      841 gcgctcgatg agcgccatgc ccggcacgtg ctgcccggcg gcggcatcct gaacgctacc
      901 gtcctcgttg acgggcgcct cgtcggagtc tggcagcttc accgcgacaa gaagcgggcg
      961 accatgacgg tcgccccgtt tgagccgctg ccgcccgaga tctacgccgc catcgaggtg
     1021 gaaacggacg acctcggacg gttcctcgac atgcccgtcg cggtcacgtt cggcgcaccg
     1081 ctctcgagcg gcagctagcc cccggcgctg gcgcggaacg aagccagcgc cctggcctca
     1141 tcctcagggc tcatgcgcat gaacagcgcc gaggactcga cgagcacctc gcccgcctcg
     1201 ttcgtcaggc tcgcctcgac ctcgatgcgc cgcccgcggc tgcgcatgat gcgcgcacgg
     1261 gccgtcaccg gcgcgtcgag gccaaccgcg cggcggaagc gcacttccat cttcgcggtg
     1321 acggcgaaga cgccatccgc atacatcgcc cagcccatcg cctcgtcgag cagcagcgag
     1381 agcagccccc cgtgcatgac ctcggggaac ccctggtcct cgggacgcgg gtcatagcgt
     1441 gcgacgaccg cgtcgccttc gcgctcgaac acgaggtgaa agccaatcgg attgtgccgc
     1501 ccgcacccga aacagtagtg ctcgcggagc gcggggtccg tcccggtcgt catgccgacg
     1561 tcccacgaga aacagaggcc gtcccggcga gcagcaccgt accgtcttcg gcctcgcaac
     1621 tcacgtcata ggtcgccacg ccatcgctcg aggagcgcag cagcgcccgc gcggacacat
     1681 tgaccggggg gaccgccggc gcccgccaac gcaccttcag cgctcccgaa cgtgcccatg
     1741 cctcaccgaa ggccgcgctc atcaactcgg acaccagcgc gagcacgagc atgccgtggg
     1801 cgatccgcgt cccgaacggg gaggcggccg cgaacacctc gtcaacgtgg atcgggttgt
     1861 ggtcgcgtgc ggcatcggcg taggcgtcaa cgcgcccctg gtcgatgagg cggacgaggc
     1921 gcagttcctc gggcggcgtg ctcatcctgc ctccgaggtc gccacgggcg ccatcaccgt
     1981 cgtgcgcccg cgcagcacga cacgttcgcc cgctttcagc tcgctggcga agaccacgat
     2041 gttcgagccc tgccgctccg actgctgcgc gaccgtcagt tccgcctcga gaggctcgcc
     2101 ctgggccacc gtgtcgagga actcgaactc ctgcccggcg tgcagcgccc cgggaggcag
     2161 aggcatcacc tccatcagtc ccgcgaggat gtacgccccc agcgcgagcg gtggaacgtg
     2221 cgccgaccac gtgcccgcag cctcccccgt cgccgcgaga tacgcgcgga cgtcctcgag
     2281 ggtcacggtg aacgcgatgg gcgggagccg gtcgccgcgc ctgagcgcgt ggaagtcgat
     2341 gctcactggc cgcgattat
//
LOCUS       DAORPG010000299         2356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5282_length_2356_cov_3.222947, whole genome shotgun sequence.
ACCESSION   DAORPG010000299 DAORPG010000000
VERSION     DAORPG010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5282_length_2356_cov_3.222947"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="PLX85_06940"
     CDS             <1..1092
                     /locus_tag="PLX85_06940"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC62943.1"
                     /translation="SFAALGFMSLANGLSNIVFSNIGGVMADRVARKQSLVQTGQVMS
                     AVTAFVMVVIIAAGWLHFWHVVAAAMLLSGAHILTMPARQSLTPDVAGMHRLQNAAAL
                     YTFGQNTATLLMPATAGFILGAAGGDAPAAKYVYAIICGVQFGAALLLGRVRVPPREV
                     SGARRAILGELIAGLRYVRHHPQIRLILGFHIFAAMFVATYMSLLPGFAKEVLGVGAS
                     RLGLLQAMLGLGAISGALIVGSLPPRRRGTVLLVAVVWLGAALAVMSLITSYTLALPA
                     AVLIGMGQTGYLTMCPVLLQTYVDPEFRGRVLSIYLMQFGLWSIGIFGIGLLANAAGP
                     QTAMRVSAVLLAGGALTMLAFVRSFREID"
     gene            1186..1992
                     /locus_tag="PLX85_06945"
     CDS             1186..1992
                     /locus_tag="PLX85_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRC62944.1"
                     /translation="MRTQVTLSTDHLIYEKDAEKKRAYITFNRPDRMNAISPEMNRGI
                     SEAMDDFNSDPEMWIVIFTGAGERAFCAGADLGSTIPNATANARSEPLQVNEHRSFDR
                     VFKPILAAVNGFCIAGGLEMMQGMDIRIASENATFGLGEVRWGIVPSGGSHVRLPRQI
                     PWAWAMELLMTGQRITAQQAYNCGLVNRVVAPDQLMAETERFADMICDNGPLAVRTAK
                     EIAVRASMGMSWEQGFALESVLATRAFGSEDAVEGPKAFMEKRKPNFKGR"
     gene            complement(2016..>2356)
                     /locus_tag="PLX85_06950"
     CDS             complement(2016..>2356)
                     /locus_tag="PLX85_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HRC62945.1"
                     /translation="KHFSWEPRGKRRIHTTPSAREIEACQPWLQAEIEVVRPQVLVCL
                     GATAAKALLGPGVSVTRQRGRFIESLLAPHVTLTVHPSSVLRERDEALRRLAMERLVA
                     DLRVVLPHLD"
BASE COUNT          364 a          724 c          891 g          377 t
ORIGIN      
        1 tcgttcgccg cgcttggttt tatgtcgctc gccaacgggc tgtcgaacat tgtcttctcg
       61 aacatcggcg gggttatggc ggaccgcgtg gcgcggaagc agtcgctcgt gcagaccggg
      121 caggtcatga gcgcggtgac cgccttcgtc atggtggtca tcatcgcggc ggggtggctg
      181 cacttctggc acgtagtggc agcggcgatg ctgctgagcg gagcgcacat cctcacgatg
      241 cccgcgcggc agtctctgac gcctgacgtc gccgggatgc accggctgca aaacgcggcg
      301 gcgctgtaca ccttcgggca gaacacggcg acgctgctga tgccggcgac ggccgggttc
      361 atcctcgggg cggcgggcgg cgacgcaccg gcggcgaagt acgtctacgc catcatctgc
      421 ggggtgcagt ttggcgcggc actgctgctc ggacgggtgc gggtgccgcc acgtgaggtc
      481 agcggagcga ggcgggcgat cctcggcgaa ctgatcgcgg ggctgcgcta cgtgcggcat
      541 caccctcaga tccggctcat cctcgggttt cacatcttcg cggcgatgtt tgtggcgacg
      601 tacatgtcgc tgctgcccgg gttcgcgaag gaggtcctgg gcgtgggcgc gagccgcctc
      661 gggctgctgc aggcgatgct ggggctgggg gcgatcagcg gcgccttgat cgtggggtcg
      721 ctgccgcctc gacggcgagg aacggtgctg ctcgtggccg tggtgtggct cggggcggcg
      781 ctggcggtga tgtcgctcat cacctcgtac acgctggcgt tgccggcggc ggtgctgatc
      841 gggatggggc agaccgggta cctgacgatg tgcccggtgc tgctgcagac gtacgtcgac
      901 cccgagttcc gtgggcgggt gctcagcatc tacctgatgc agtttgggct gtggtcgatc
      961 ggcatcttcg ggatcggcct gctcgcgaac gccgcggggc cacagaccgc gatgcgggtg
     1021 tccgcggtgc tgctcgcggg cggggcgctc acgatgctcg cgttcgtgcg gagcttccgc
     1081 gagatcgact gacaccgccg gacaccgtcg tcgccacccg gcgcacccgg acgtggcata
     1141 ctcccgcgcg aatcacctcg gacggcgcat agaaggagcg agcacgtgcg gacgcaggtc
     1201 accctgtcga cggaccacct gatctacgag aaggacgcag agaagaagcg agcgtacatc
     1261 acgttcaacc ggccggaccg gatgaacgcg atcagcccgg agatgaaccg tggcatcagc
     1321 gaggccatgg acgacttcaa cagcgacccc gagatgtgga tcgtcatctt cacgggggcg
     1381 ggcgagcggg cgttctgcgc gggcgcggac ctcggcagca cgatcccgaa cgcgacggcc
     1441 aacgcgcggt cagagccact gcaggtgaat gagcaccgct ccttcgaccg ggtgttcaag
     1501 ccgatcctcg cggcggtgaa cgggttctgc atcgcgggcg ggctcgagat gatgcagggg
     1561 atggacatcc gcatcgcctc ggagaacgcg acgttcgggc tcggcgaggt gcgctggggc
     1621 atcgtgccct cgggcggctc gcacgtgcgg ctgccgcggc agatcccgtg ggcgtgggcg
     1681 atggagctgc tgatgacggg gcaacgcatc acggcgcagc aggcgtacaa ctgcggcctc
     1741 gtgaaccggg tcgtggcgcc tgaccagctg atggcggaga ccgagcggtt cgcggacatg
     1801 atctgcgaca acggcccttt ggcggtgcga acggccaagg agatcgcggt gcgcgcctcg
     1861 atggggatgt cgtgggagca gggcttcgcg ctggagtcag tgctcgcgac gcgcgcgttc
     1921 ggcagcgagg acgccgtcga agggccgaag gcgttcatgg agaagcggaa gccgaacttc
     1981 aagggacgat gagccccgac ctgcctcgat gggagtcagt cgaggtgggg gaggaccaca
     2041 cgcaggtcag cgacgaggcg ctccatcgcc aggcgacgga gcgcctcgtc acgttcccgc
     2101 agcaccgacg acgggtggac ggtgagggtg acgtgcggcg cgaggagcga ctcgatgaag
     2161 cgcccgcgct ggcgggtgac gctcacgccg gggccaagca acgccttcgc cgccgtggcg
     2221 ccgaggcaaa cgagcacctg cgggcggacc acttcgatct ctgcctgtag ccagggctgg
     2281 caggcctcga tctcgcgggc gctcggggtc gtgtggatgc gacgcttacc tcgaggctcc
     2341 cagctgaagt gcttca
//
LOCUS       DAORPG010000300         2354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5289_length_2354_cov_3.837756, whole genome shotgun sequence.
ACCESSION   DAORPG010000300 DAORPG010000000
VERSION     DAORPG010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5289_length_2354_cov_3.837756"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..597
                     /locus_tag="PLX85_06955"
     CDS             148..597
                     /locus_tag="PLX85_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62946.1"
                     /translation="MNEPETAPVKRETQFADSNDGAEIPSPDAAPLYARQDDADHPET
                     PAELHVAPVLPAEPGAATEGLLRGIEEGGLLSYALAERFGALEHCDRCGRPIIEVPTL
                     ATALATHYSVGDEERLAFEDAIRRSSLEQANESSPNFCSYHGQITSE"
     gene            complement(606..1388)
                     /locus_tag="PLX85_06960"
     CDS             complement(606..1388)
                     /locus_tag="PLX85_06960"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC62947.1"
                     /translation="MSELAPNYALLDLVGAAGMMFFPRPDPSAPPPGASDLAIEVGAD
                     VTVNARFYAFDGTLPTVLYFHGNGEVAGDHDALAPLYRQAGANLFVAEFRGYGRSTGR
                     PTVEHLVSDAHPVTAFFHAHLDGLGFNERRFVMGRSLGTHPALEVAANASERIRGLIV
                     ESGAGGLRRMLQRFGFDVQQGEGADLVEAHEAKIRSIQMPLLVIHGEWDELIPLQQAV
                     ELYEMLPHVERELLIIPGAGHNDLLWAGLQDYFGAIQRFVSR"
     gene            1700..>2354
                     /locus_tag="PLX85_06965"
     CDS             1700..>2354
                     /locus_tag="PLX85_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HRC62948.1"
                     /translation="MNGSAPRPRRAALGDIGPVGSPAAVESAAVEERVEAGQQSQVAA
                     TEDILGVARLNDPELVRRFEAMLSGVSPDDLRVLRDALLEDSSPLPVSADDELAADWR
                     EGAYPYKNLMSRRNYEKQKYRLQVELLKLQAWVKETGQRVVILFEGRDAAGKGGTIKR
                     FMEHLNPRGARVVALEKPTEIERGQWYFQRYVQHLPSHGEIVMFDRSWYNRAGVERVM
                     "
BASE COUNT          422 a          845 c          741 g          346 t
ORIGIN      
        1 acgaccgagg aggtagcgcc tccgggctcc tcgctatcac ccgcctgcca atcgggtcgg
       61 caggcgaccg tgactcgatc gacgcgctgc acacgcctct gcaagctccc gctccgtacc
      121 gttcgagaag cgcgagggga gaaggcgatg aacgaacccg agacggcgcc ggtgaagcgc
      181 gagacgcagt tcgcggactc gaacgatggg gccgagatcc cgtctccgga tgcggccccg
      241 ctctacgccc gccaggacga cgccgaccat cctgaaacgc ctgcggagct ccacgtcgcg
      301 cccgtattgc ccgccgagcc cggtgcggcg accgagggcc tgctacgcgg tatcgaggag
      361 ggcgggctcc tctcctatgc cctcgcggag cggttcggcg cgctcgagca ttgcgaccgc
      421 tgcggccgcc ccatcatcga ggtgcccacg ctcgccaccg cgctggcgac ccactacagc
      481 gtgggggacg aagagcgcct cgccttcgag gatgccatcc gtcggtcgag cctggagcag
      541 gccaacgaga gctcaccgaa cttctgtagc taccacggac agatcacgag cgaatagaac
      601 ggccttcagc ggctgacaaa ccgctggatc gccccgaagt agtcctgcaa cccggcccac
      661 agcaggtcgt tgtgccccgc gccggggatg atcaacagct cacgctcgac gtgcggcaac
      721 atctcgtaca actcgactgc ctgttgcaat gggatcagct cgtcccactc cccgtggatc
      781 acgaggagcg gcatctggat ggaccggatc ttcgcctcgt gcgcctcgac gagatcggcg
      841 ccctcgccct gctgcacatc gaagccgaaa cgctgcaaca tccgccgcag gccgcctgcc
      901 ccgctctcca cgatgagccc gcggatacgt tccgaggcgt tcgccgccac ctcgagggcc
      961 gggtgcgtgc cgaggctgcg gcccatcacg aagcgccgct cgttgaaccc cagcccgtcg
     1021 aggtgcgcgt gaaagaacgc cgtcaccgga tgcgcatcgg agacgaggtg ttcgacggtc
     1081 ggacggccgg tactgcgccc gtatccccgg aactccgcaa cgaagaggtt cgcccctgcc
     1141 tgccggtata gcggcgcgag cgcgtcatgg tcgcccgcca cctccccgtt cccatggaaa
     1201 tacaggacgg tcggtagcgt cccatcgaac gcgtagaacc gcgcattcac ggtcacgtcc
     1261 gcgcccacct cgatcgcgag gtccgaggcg cccgggggtg gtgctgacgg gtccggccgc
     1321 gggaagaaca tcattccggc cgcacccacc aggtcgagca atgcgtaatt gggggccaat
     1381 tcggacattg gcagacctcc cgcccctcgc cggttgagag gcaacataca cctcggcgct
     1441 acacgttact ggtgccctgc gtcctgcgag cgcgctcgct cgccctcatg ctcgcacatc
     1501 tcggcgcgaa cctcgtccgt cttcgtcgga taatgaagaa gagacccgaa ggagcctcat
     1561 aatgacgacg cctcgcgaac cacgcccctc ccgcgccaac ggtgccgcag ccctgaatgg
     1621 ccacggcccc gatgcagccc ccggtccccg tggacgcagg cgttccaacg gcacgaacgg
     1681 caatagcaat ggcaacggca tgaacggcag cgccccccgt ccccggcgcg ccgcgctcgg
     1741 agacatcggg ccggtcggct ccccggccgc cgtcgaatca gcggcggtgg aagaacgcgt
     1801 cgaggcaggc caacagtctc aggtggccgc caccgaggat atcctcggtg tggcacgtct
     1861 gaacgatccc gaactggtgc gccggttcga ggcaatgctt tccggcgtct ctcccgacga
     1921 cctccgcgtg ctccgcgacg ccctgctcga ggattcctca ccgctgccag tcagcgccga
     1981 tgacgagctc gccgccgact ggcgcgaggg cgcttacccg tacaagaacc tcatgtcgcg
     2041 ccgcaactac gagaagcaga agtaccggct gcaggtggag ctgctcaagc tgcaggcctg
     2101 ggtgaaagag accggccagc gcgtcgtcat cctcttcgag ggccgtgacg ccgccggcaa
     2161 gggaggcacg atcaagcgct tcatggagca cctcaacccc cgaggcgctc gcgtcgttgc
     2221 gctcgagaag ccgaccgaga ttgagcgcgg gcaatggtac ttccagcgct atgtgcagca
     2281 cctcccctcg cacggcgaaa tcgtgatgtt cgaccgttcc tggtacaacc gcgccggcgt
     2341 cgagcgcgtg atgg
//
LOCUS       DAORPG010000301         2352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5303_length_2352_cov_3.118851, whole genome shotgun sequence.
ACCESSION   DAORPG010000301 DAORPG010000000
VERSION     DAORPG010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5303_length_2352_cov_3.118851"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLX85_06970"
     CDS             <1..228
                     /locus_tag="PLX85_06970"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRC62949.1"
                     /translation="DPRFFLGALSHAGARAEAALMVGDRLDNDYEPARAAGMHAVLLD
                     REDRVEDASVVRIRVLTELLEMVEREAPRAG"
     gene            complement(232..1164)
                     /locus_tag="PLX85_06975"
     CDS             complement(232..1164)
                     /locus_tag="PLX85_06975"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HRC62950.1"
                     /translation="MSQTYGGQAVVEGVMIRGARAMAVAVRRPDGSIALRADRLGNIY
                     TSALRRIPLIRGVIVLWETIALGMKALAWSASVATEEVDEHGEPQSLGLFSWLVMGVS
                     MAVGVALFFVIPVVATAWLEGQLPSFFVVLIEGILRLAMLIGYIWLIGRSDEIGRVFQ
                     YHGAEHMAIHAYEEGRELTVQAIRRFPKEHPRCGTSFLLTVGVVAVFVFALLGSPPLW
                     WRITSRIVLVPVIASVAYEAIRFAGFHQRWPLVRWLFAGNIALQYLTTRVPEDEHIQV
                     AIASLEHAVAEDDRAARGIPPLAEPALAERRATD"
     gene            complement(1214..1406)
                     /locus_tag="PLX85_06980"
                     /pseudo
     CDS             complement(1214..1406)
                     /locus_tag="PLX85_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942123.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1622..1828)
                     /gene="rpmE"
                     /locus_tag="PLX85_06985"
     CDS             complement(1622..1828)
                     /gene="rpmE"
                     /locus_tag="PLX85_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRC62951.1"
                     /translation="MKAAIHPEYVDSIVHCSCGKEWHTRSTKPEMRVDVCSNCHPFFT
                     GEQRIVDTAGRVERFRRRYAKKEA"
     gene            complement(1878..2141)
                     /gene="rpmA"
                     /locus_tag="PLX85_06990"
     CDS             complement(1878..2141)
                     /gene="rpmA"
                     /locus_tag="PLX85_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020410273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRC62952.1"
                     /translation="MAHKKSGGSSKNGRDSNAQRLGVKRFDGEVVTAGSIIVRQRGTR
                     VHPGENIGVGRDHTLWAKVDGSVKFSPFAKGRRKQVSIVVAEA"
     gene            complement(2163..>2352)
                     /gene="rplU"
                     /locus_tag="PLX85_06995"
     CDS             complement(2163..>2352)
                     /gene="rplU"
                     /locus_tag="PLX85_06995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRC62953.1"
                     /translation="RDGAVQVGSPTVAGASVAATVKDHPRDEKVLVFKYKNKTRRRQL
                     RGHRHQRTRLAITAIHAG"
BASE COUNT          362 a          751 c          871 g          368 t
ORIGIN      
        1 gacccgcgtt tcttcctcgg agcgctctcg cacgcggggg cgcgggccga agctgccctg
       61 atggtcggcg accgcctcga caatgactac gagccggcgc gtgcggcggg gatgcacgcc
      121 gtcctactcg accgcgagga ccgcgtcgag gacgcctcgg tggtgcgcat tcgcgtcctc
      181 accgagttgc tggagatggt cgagcgggag gcgccgcgcg ccgggtaggg gtcagtccgt
      241 ggcgcggcgt tccgcgagcg ccggttcagc cagcggcgga atgccgcgag cggcccggtc
      301 gtcctcggcg accgcgtgtt cgagcgaggc gatcgcgacc tggatgtgct cgtcctcggg
      361 gacgcgggtt gtcaggtact gcagcgcgat gttgcccgcg aagagccagc gcacgagcgg
      421 ccagcgctgg tggaagccgg cgaagcgaat cgcctcgtag gccacggagg cgatgaccgg
      481 cacgagcacg atgcgggagg tgatgcgcca ccacagcggc ggggagccga gcagcgcaaa
      541 cacgaagacg gcgacgacgc cgaccgtgag caggaacgac gtgccgcagc gggggtgctc
      601 cttggggaag cgccggatgg cctgcaccgt gagctcacgg ccctcctcgt aggcgtggat
      661 ggccatgtgc tcggcgccgt ggtactggaa gacgcggccg atctcatcgc tgcggccgat
      721 gagccagata tagccgatga gcatcgcgag ccggagtatg ccctcgatca gcacgacgaa
      781 gaacgagggc agctgtcctt caagccaggc ggtggcgacg accgggatga cgaagaagag
      841 cgcgacgccg acggccatcg agacgcccat gacgagccag gagaacaggc caagcgactg
      901 cggctcgccg tgctcgtcga cttcctcggt ggcgacggag gcggaccagg cgagggcctt
      961 catgccgagc gcgatcgtct cccagaggac gatcacgccg cggatgagcg ggatgcggcg
     1021 cagtgcactg gtgtagatgt tgccgaggcg atcagctcgg agggcgatgg agccatccgg
     1081 gcggcgcacg gcgacggcca tggcgcgcgc gccacggatc atgacgccct cgacgacggc
     1141 ctgcccaccg tatgtctggc tcacgatccc agcctacgat cccgcgtgtc tgacgacgag
     1201 ggcgggcgag gggttagttg gccagatcgc cgactgcccg ttcaaccagc gcggcgattc
     1261 gagcttcctc ggccggggtg agcgctctca gcgcgaacga ggtggggcca cattgcgccg
     1321 tcgtccaggt gcgcggcgtc gttgaacccg aacgtggcgt aacgggccac gaacttctcc
     1381 gcgccctgga agaagcagac gacccggtga aggcgccgcc tgtgccctcg ctcttcgagg
     1441 cagcacgcac gctgctcctg gccgtcttcg cttcgggcac gtcggacctc cggcctcacc
     1501 ggccgccctg gccagtcgct cgtgttcagg gtagcggccg acgcgagcga atccggattg
     1561 cgcaggaacg acgaaggcgg ccgttcggcc gccctcgaaa cgatgcttcc tgagattcgg
     1621 ctcaggcttc cttcttggcg tagcggcggc ggaagcgctc gacgcgaccg gcggtgtcga
     1681 cgatgcgctg ctcgccggtg aagaagggat ggcagttcga gcagacgtcg acgcgcatct
     1741 cgggcttggt ggagcgggtg tgccactcct tgccacacga gcagtggacg atggagtcga
     1801 cgtactcggg gtggatagcg gccttcattg ggttcgtact ccggtttctg gtttcgcgtc
     1861 gcccccggcg aggctggtta cgcctcggcg acgacgatcg agacctgctt gcggcggccc
     1921 ttggcgaagg ggctgaactt gacggagccg tcgaccttcg cccagagggt gtggtcacgg
     1981 ccaacgccga tgttctcgcc cgggtgcacg cgggtgccgc gctggcggac gatgatggag
     2041 ccggcggtca cgacctcgcc atcgaagcgc ttcacgccga ggcgctgcgc gttcgaatcg
     2101 cggccgttct tgctggagcc gccggacttc ttgtgagcca tgtgttgctc ctcgtgggcc
     2161 cgctagccgg cgtggatcgc ggtgatggcg agccgggtgc gctggtgacg gtggccccgc
     2221 agctggcgac ggcgtgtctt gttcttgtac ttgaacacga gcactttttc gtcgcgcggg
     2281 tggtccttga cggtcgcggc caccgaggcg ccggcaacgg tcggcgagcc gacctgcacg
     2341 gccccatcgc gg
//
LOCUS       DAORPG010000302         2347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5330_length_2347_cov_3.341623, whole genome shotgun sequence.
ACCESSION   DAORPG010000302 DAORPG010000000
VERSION     DAORPG010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5330_length_2347_cov_3.341623"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="PLX85_07000"
     CDS             <1..857
                     /locus_tag="PLX85_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRC62954.1"
                     /translation="VAATTMLALAGMVVALDAYGPVTDNAGGIAEMSELPDEVREVTD
                     ALDAVGNTTKAVTKGYAIGSAGLAALVLFAAYTEDLGRYFPDLNVAFVLSDPYVVIGL
                     FLGGLLPYLFGAMGMMAVGRAGGAVVVEVRRQFQEKPGIMAGTDRPDYSRAVDMLTRA
                     AIREMIVPSLLPVLAPVVLYFIVRVVEDQAAAFAAVGAMLLGSIVTGLFVALSMTSGG
                     AAWDNAKKLIETGEYGGKGSEAHKAAVTGDTVGDPYKDTAGPAVNPMIKITNIVAILL
                     LAIIHNVT"
     gene            complement(935..1354)
                     /locus_tag="PLX85_07005"
     CDS             complement(935..1354)
                     /locus_tag="PLX85_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62955.1"
                     /translation="MRLSMTSWPLFLKKTAAVFALSLLVVPLVGMKGGVPATVYLIGL
                     VALHLFVLGIYFYRIRFRELDHDLRSLIARVAALAFVGYLLVAVSDFDESTPRSTLLT
                     QMLVVSLLHTVVLVLLMVRISRSAAFTPSVSDEPAEG"
     gene            1535..1611
                     /locus_tag="PLX85_07010"
     tRNA            1535..1611
                     /locus_tag="PLX85_07010"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1569..1571,aa:Glu,seq:ctc)
     gene            1727..>2347
                     /locus_tag="PLX85_07015"
     CDS             1727..>2347
                     /locus_tag="PLX85_07015"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRC62956.1"
                     /translation="MAASATFGVMRIGVLSDTHLPAEVPTLDELGAHSAEFLASMDLI
                     IHAGDVVLPSVLDWCAQFAPVVCAQGNHDHFHDERMSSVVVVEHEGWRIGAVHDVEGI
                     PPYVNTVEDLKREVYRDTTLDILVAGDSHYERLEYRDSTLLLDSGSPIFPHHKSTRLG
                     SMALIEVTRHGGRAEIVPLGETAERPNPCTHAAMEFDRNGLISASIG"
BASE COUNT          400 a          722 c          858 g          367 t
ORIGIN      
        1 cggtggcggc gacgacgatg ctcgcactgg cgggcatggt ggtcgccctc gacgcgtacg
       61 gccccgtgac cgacaacgca ggcggcatcg ccgagatgtc cgagctgccg gacgaggtgc
      121 gcgaggtcac ggacgccctc gacgcggtcg gcaacacgac gaaggcggtc accaagggtt
      181 acgccatcgg ctcggccggc ctggcggcgc tcgtcctgtt cgcggcctac accgaggacc
      241 tcgggcgcta cttccccgac ctgaacgtgg cgttcgtgct gagcgacccg tacgtggtca
      301 tcggcctgtt cctcggcggc ctgctgccgt acctcttcgg cgccatggga atgatggccg
      361 tcggtcgcgc cggcggcgcg gtcgtcgtcg aggtccggcg tcagttccag gagaagccag
      421 ggatcatggc tggcaccgac cgcccggact acagccgcgc ggtcgacatg ctgacccggg
      481 cggcaatccg cgaaatgatc gtgccgtcgc tcctgccggt actggcgccg gtggtgctct
      541 acttcatcgt gcgcgtagtg gaagaccagg cggccgcctt tgcggcggtg ggcgcaatgc
      601 tcctcgggtc gatcgtgacg ggcctgttcg tcgcgctctc gatgacctcg ggcggcgctg
      661 cctgggacaa cgcgaagaag ctcatcgaaa ccggcgagta cggcgggaag ggctcggagg
      721 cgcacaaggc ggcggtcacc ggtgacacgg tgggcgaccc gtacaaggac acggcaggcc
      781 ccgcggtgaa cccgatgatc aagatcacga acatcgtggc gatcctgctg ctggcgatca
      841 tccacaacgt cacgtagcgt cccgtcttcc gcggacatcg attcgaggcc ggggtgaccc
      901 ggcctcgaac gtttccggcg gggacggccg gaagtcagcc ctcggcgggc tcgtcggaga
      961 cggacggggt gaaggcggcg gaccgcgaga tgcggaccat gagcagaacg aggacgacgg
     1021 tgtggaggag cgagacgacg agcatctggg tgagtagggt gctgcgcggc gtggactcgt
     1081 cgaagtcgga cacggcgacg agcaggtagc cgacgaaggc gagcgcggcg accctcgcga
     1141 tcagcgagcg gaggtcgtgg tcgagttctc ggaagcggat gcgatagaag tagatgccga
     1201 gcacgaacag atgcagggcg acgaggccga tgaggtagac ggtggcgggg acgccgccct
     1261 tcatcccgac gagcgggacg acgaggagcg agagggcgaa gaccgccgcc gtcttcttga
     1321 ggaagagcgg ccagctggtc atcgaaagtc gcacggtccc tcctcagatc accctatgag
     1381 gcgtcctcgc cgggggcaac gaaggtgggg caggatgtgg gaaatcctcg gcaaagctac
     1441 ggctggggag cggcgatgca gcgaccgccc agggcggagc ggcgtccggt tgagtgatgc
     1501 ctcggaggtt gttaggctga gagtccactc acggggcgca ttcgtctagt ggcccaggac
     1561 accgccctct caaggcggag atcgcgggtt cgaatcccgc atgcgctacc aacttcttcg
     1621 tcggcacctt ccaactgaac agcccgcggt tgccgacgcg gcgtcgcggg cccgcctgcc
     1681 gatctcgaac ctgtactgtg actgcggagc gaacgtacgc cgggtagtgg cggcatctgc
     1741 taccttcggc gtgatgcgta tcggggtgct gtccgacact cacctgcccg ccgaggtgcc
     1801 aacgctcgat gaactggggg cgcactcggc ggagtttctg gcctcgatgg acctgatcat
     1861 ccatgcgggc gacgtggtgc tgccctcggt gctcgactgg tgcgcgcagt tcgcgccggt
     1921 ggtgtgtgcg caggggaacc acgaccactt ccacgacgag cggatgtcct cggtggtggt
     1981 ggtggagcac gaggggtggc gcatcggcgc ggtgcacgac gtcgaaggca tccctcctta
     2041 tgtcaatacc gtagaagacc tgaagcgcga ggtgtatcga gacacgaccc tcgacatcct
     2101 cgtagcgggc gattcgcact acgagcggct ggagtaccgc gactcgacgc tgctgctcga
     2161 ctcggggagc ccgatctttc cgcaccacaa gtcgacgcgt ctggggtcga tggcgctgat
     2221 cgaggtgact cggcacgggg ggcgtgcgga gatcgtgccg ctgggcgaga ccgccgagcg
     2281 gccgaacccg tgcacgcacg cggccatgga gttcgaccgc aacggattga tcagcgcttc
     2341 catcggc
//
LOCUS       DAORPG010000303         2339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5360_length_2339_cov_3.309107, whole genome shotgun sequence.
ACCESSION   DAORPG010000303 DAORPG010000000
VERSION     DAORPG010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5360_length_2339_cov_3.309107"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1547
                     /locus_tag="PLX85_07020"
     CDS             <1..1547
                     /locus_tag="PLX85_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRC62957.1"
                     /translation="PASTPMSETEPAITTETPSSEAGASDSLPRSSHPLDPIFHPRGV
                     AVVGVSSRADPEGRMGGIFLNALVMQGYPKEYLYPVNPKMDEVLGIKCYPSVLECPDP
                     VDHVISQIPAAGLAELVDQCIQKGVRSIHSFTAGLAETGEPELIEAEREIIEKVKAAG
                     VRFIGPNCMGLYVPGVGLSFMPMLPEDPGNVFMLSQSGANANTILRSLGERGLRFSKG
                     VSFGNGADVRAHELLDYALADPQSEYVVGYMEGVQDARRFFERLKQVAVAKPTIILKG
                     GITSDGARAAHSHTGSLAGSIEIFDAMCRQAGAWRAESMDELLDLATMVTTSVRDVKG
                     PNVALIGAGGGFAVLSSDQLALVGLTLPPLPEEVQAELREFVPVAGTSVRNPVDVTFG
                     RDHPLDFVSEPDLTRRLFEVIANSHSTDVILTTVGGPWRPGSDTSRDEEDRHFRAAIE
                     EFSRIQERTGVPIVMVQDERRVRRVTQFAHQKRIAIVPTVTRAGKGIADLLAWRKERE
                     GLPSIL"
     gene            1608..>2339
                     /locus_tag="PLX85_07025"
     CDS             1608..>2339
                     /locus_tag="PLX85_07025"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC62958.1"
                     /translation="MDWEDSAEQAAFRQDVRSFIQQRLPEFYRRPKEDDDPETDWQHD
                     MFLGTDDAKAAAREWIDALAERGWSAPHWPKEYGGGGLSTIEQFIFKQELALAHAPEV
                     GGVNGVGQFGPTLLVHGTEEQKKKFLPPTLTGEYLWAQGYSEPGAGSDLASLQTRAVR
                     EGDEWVVNGQKIWTSNAHKSNWIFMLVRTDPDAPKHRGISFLFTSMEVPGLTVRPIIS
                     AGWRHATNETFYEDVRIPADQIVGEV"
BASE COUNT          393 a          837 c          756 g          353 t
ORIGIN      
        1 gacctgcgtc gaccccgatg agtgaaacgg agccggccat caccaccgag accccttctt
       61 cagaagccgg cgcctcagac tcgcttcccc gcagctccca tcccctcgac ccgatctttc
      121 acccacgcgg cgtcgccgtc gtcggggttt cctcgcgcgc ggaccccgag ggacgcatgg
      181 ggggcatctt cctgaacgcc ctcgtcatgc agggctaccc gaaggagtac ctctacccgg
      241 tcaacccgaa gatggacgag gtgcttggca tcaagtgcta cccgagcgtc ctcgagtgcc
      301 cggaccccgt cgaccacgtg atttcccaga tcccggcggc cgggctcgcc gagctggtcg
      361 accagtgcat ccagaaaggc gtgcgttcga tccactcgtt cacggccggc cttgccgaga
      421 ccggcgagcc cgagctgatc gaggccgagc gcgagatcat cgagaaggtg aaggccgcgg
      481 gcgtccgctt catcggcccc aactgcatgg gcctctacgt ccccggcgtc ggcctctcgt
      541 tcatgccgat gctgccggag gaccccggca acgtgttcat gctctcgcag tccggcgcca
      601 atgcgaacac catcctgcgc agcctcggcg aacgtggcct ccgcttctca aagggcgtct
      661 cgttcggcaa cggcgccgac gtgcgagcgc acgagctgct cgactacgcc ctcgccgacc
      721 cgcagtcgga atacgtcgtc ggctacatgg agggcgtcca ggacgcccgc cggttcttcg
      781 aacggctcaa gcaggtggct gtcgcgaagc cgaccatcat cctcaaaggt ggcatcacct
      841 ccgacggcgc gcgcgccgcg cactcgcaca ccggctcgct ggcgggctcc atcgagatct
      901 tcgacgccat gtgccggcaa gccggcgcct ggcgcgccga gagcatggac gagctgctcg
      961 acctcgcgac gatggtcacg acctcagtgc gcgacgtgaa gggcccgaac gtggcgctga
     1021 tcggcgccgg cggcggcttt gccgtgctct cctcggatca gctcgcgctg gttggcctca
     1081 cactgccgcc gctgcccgag gaggtgcagg ccgaactgcg cgagtttgtg ccggtcgccg
     1141 gcacgagcgt ccgcaacccg gtcgacgtca ccttcggccg cgaccaccca ctcgacttcg
     1201 tgagcgagcc cgacctcacg cgccgcctct tcgaggtgat tgcgaactcg cactcgactg
     1261 acgtcattct cacgaccgtc ggcggcccct ggcggcccgg cagcgacacc agccgcgatg
     1321 aagaggaccg tcacttccgc gcggcgatcg aggagttctc gcgcatccag gagcgcactg
     1381 gcgtgcccat cgtcatggtg caggacgagc ggcgcgtgcg ccgcgtcacc cagttcgcgc
     1441 accagaagcg catcgccatc gtgccgaccg tgactcgcgc cggaaagggc atcgcggacc
     1501 tcctcgcgtg gcgcaaggag cgcgagggcc tgccctcgat cttgtaactg cacgaggccc
     1561 gaccacgcct ataatcctcg cgacgacacg gcgaggagga ggcgacgatg gactgggaag
     1621 acagcgccga gcaggcggcg ttccggcagg acgtgcgttc gttcatccag cagcgcctgc
     1681 cggagttcta ccggcggccg aaggaggacg acgaccccga gaccgactgg cagcacgaca
     1741 tgttcctcgg gaccgacgat gcgaaggcgg ccgcgcgcga gtggatcgat gcgctcgctg
     1801 agcgcggctg gagtgcgccc cattggccga aggagtacgg cggcggcggg ctctccacga
     1861 tcgagcagtt catcttcaag caggagctcg ccctcgccca tgcgcccgag gtcggcggcg
     1921 tgaatggcgt cggccagttc ggccccaccc tcctcgtgca cggcaccgag gaacagaaga
     1981 agaagttcct gcctccgacc ctgaccgggg agtacctctg ggcgcagggc tactcggagc
     2041 ccggcgcggg ttccgacctt gcctcattgc agacgcgcgc cgttcgcgag ggcgacgagt
     2101 gggtcgtcaa cggccagaag atctggacct cgaacgccca caagtcgaac tggatcttca
     2161 tgctcgtgcg caccgacccg gacgccccga agcatcgagg catctcgttc ctcttcacga
     2221 gcatggaagt gcccggcctc accgtgcgcc cgatcatcag cgccggctgg cggcacgcga
     2281 ccaacgagac cttctacgag gacgtgcgca tccccgcgga ccagatcgtc ggggaggtg
//
LOCUS       DAORPG010000304         2338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5370_length_2338_cov_2.930793, whole genome shotgun sequence.
ACCESSION   DAORPG010000304 DAORPG010000000
VERSION     DAORPG010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5370_length_2338_cov_2.930793"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="PLX85_07030"
     CDS             <1..855
                     /locus_tag="PLX85_07030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HRC62959.1"
                     /translation="DTLLVRGRYQEKPALPFAPGLEAAGVVLEVAPGVQHVLPGERVV
                     ALLDHGGFAEEVVARASDVVRIPEEIDDATAAAIPIAYLTSYLALVERARLQPREVLV
                     VFGASGGVGLSAVEVGHGLGARVIAVASSHDKLATALDRGADDAVSYTEEDVAARIEE
                     LAGGADVIYDPVGGELFEAGLHAINPGGRILLVGFASGTVPQIPANHLLVKDAAALGF
                     SIGQFRRHQPDRVRAALAELLRMTVEGRLHPLVSQVLSLDRAVEGLRELQAGRTVGKL
                     VVRPRVTD"
     gene            complement(852..>2338)
                     /gene="priA"
                     /locus_tag="PLX85_07035"
     CDS             complement(852..>2338)
                     /gene="priA"
                     /locus_tag="PLX85_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRC62960.1"
                     /translation="LRHAVERGRRGLVLVPEIALTPQTVRRFLERFPGRVGVLHSGLT
                     LGEAYDEWHAAARGDYDVVIGSRSAAFAPQPDLGLVIVDEAHEWTFKQQDPAPRYDAR
                     MVAARLGTLTGAAVVYGTATPTAEQWFEAAQGDLLRLELPRRLRPVPQPGGGTWLWPA
                     EEPADVEVVDVRGARSLFSEELLRALGETLDRDEQAILFLNRRGMFGHLLCPNGHTPT
                     CPSCDVSLSLHDPPGRLVCHQCNRSRPLQSRCRECGSALRPARAGTQRVEQEVRRLFP
                     AARVERWDRDSVRTAEQHEAILGRFMRHESDVLVGTQMVAKGLDLPLVTLVGVVMADY
                     SLWAGGFRAREQTFQLLSQVAGRAGRADRLGRVVIQTLSPDDRAIEAATLGDVDGFYE
                     DELPWRREHRYPPFVRLVRLQFAHARQEYALEEATRVRQELRRLAAGLPDIDVLGPTP
                     PQIARARGRYRWALLVKGIDPAAVVRQLELPPGWVVDVDPATVT"
BASE COUNT          346 a          876 c          791 g          325 t
ORIGIN      
        1 gacacgctgc tggtgcgcgg ccgctaccag gagaaacctg cactcccatt cgcccccggc
       61 ctcgaagcgg ccggcgtcgt ccttgaggtc gccccgggcg tacagcacgt gctccccggg
      121 gaacgagtcg tcgcgctcct tgaccacggc ggcttcgcgg aagaagtggt ggcccgggca
      181 tcggacgtgg tgcgcattcc cgaggagatc gacgacgcga ccgctgccgc catcccgatc
      241 gcgtacttga cgtcctacct ggcgctcgtc gagcgcgccc gtctgcaacc tcgggaggtg
      301 ctcgtggtct tcggcgcctc gggcggggtc gggctgagcg ccgtggaggt tggccacggt
      361 ctgggggcac gcgtcatcgc cgtcgcctct tcgcatgaca agctggcgac cgcgctcgat
      421 cgcggggccg acgacgccgt cagctacacc gaggaggacg tggcggcccg catcgaagaa
      481 ctagccgggg gtgccgacgt catctacgac ccggtcggcg gcgaactgtt cgaagccggc
      541 ctgcacgcca tcaacccggg tggccgcatc ctgctcgtcg ggttcgcaag cggtaccgtg
      601 ccgcagattc cggcgaacca cctcctggtc aaggacgcgg cggccctcgg cttcagtatc
      661 gggcaattcc ggcgccacca gcccgaccga gtacgtgccg cgttggccga gttgctgcgc
      721 atgaccgtgg agggccgcct gcacccgctc gtctcgcagg tgctgtcatt ggatcgggcg
      781 gtcgagggcc ttcgcgagtt gcaggccgga cgcaccgtcg gaaagctcgt cgtccgcccc
      841 cgggtgacgg actaggtcac cgtcgccgga tcgacgtcga ccacccatcc gggcggcagt
      901 tcgagctgcc gcaccaccgc cgcggggtca atgcccttca ccagcagcgc ccagcggtag
      961 cgcccccgcg cccgcgcgat ctgaggtggc gtcggcccga ggacatcgat gtccggcaga
     1021 cccgccgcga ggcggcgcag ctcctgacgc acgcgcgttg cctcctcgag cgcgtactcc
     1081 tgccgggcgt gcgcgaactg caaccgcacg aggcgcacga acggcgggta acgatgctcg
     1141 cgacgccagg gcagctcgtc ctcgtagaac ccgtcgacgt cgccgagcgt tgccgcctcg
     1201 atcgcccggt cgtctggcga cagcgtctgg attaccaccc gcccgaggcg gtcggcgcga
     1261 cccgcccggc ccgcgacttg cgacaggagc tgaaacgtct gttcgcgggc ccggaacccg
     1321 cccgcccaga gcgagtagtc cgccatcacg acgcccacga gggtcaccag cggcaggtcg
     1381 aggcccttcg ccaccatctg ggtgcccacc aggacatccg actcgtggcg catgaaccgc
     1441 ccgaggatgg cctcgtgctg ctccgccgtt cgcacgctgt cgcggtccca ccgttcgacg
     1501 cgggccgccg ggaacaggcg acgcacctcc tgctcaaccc gctgcgtccc ggcccgagcc
     1561 ggccgcagcg cgctcccgca ctcgcgacaa cgcgattgca gcgggcgcga tcggttgcac
     1621 tggtggcaga cgaggcggcc cggcggatcg tgcaatgaca gcgagacatc gcacgaggga
     1681 cacgtcggcg tatgtccgtt cgggcacagc aggtgcccga acataccgcg ccggttgagg
     1741 aagaggatgg cctgctcatc acggtcgagc gtctcgccga gcgcccgcag tagttcctcc
     1801 gagaacaacg accgcgcgcc tcgcacgtcc acgacttcga cgtccgctgg ctcctccgcg
     1861 ggccagagcc aggtcccgcc tcccggctgt ggcaccgggc gcagccggcg cggcagctca
     1921 aggcgcagca ggtcgccctg cgccgcctcg aaccactgct cggcggtggg cgtcgccgtc
     1981 ccgtacacaa cggcggcccc ggtgagcgtc ccgagccgag cggcaaccat ccgcgcgtcg
     2041 tagcgcggcg ccgggtcctg ctgcttgaac gtccactcgt gcgcctcgtc cacgatcacg
     2101 agcccgaggt ctggctgagg cgcgaacgcc gcagagcgag agccgatcac cacgtcgtag
     2161 tcgccccggg ccgcagcgtg ccactcgtca tacgcctccc cgagcgtcag ccccgagtgc
     2221 aacaccccga cccgtcccgg gaaccgctcg aggaagcggc gcacggtctg tggcgtgagc
     2281 gcgatttcgg gcaccagcac cagcccgcgc ctgccgcgtt ccaccgcgtg gcgcaacg
//
LOCUS       DAORPG010000305         2338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5371_length_2338_cov_2.632939, whole genome shotgun sequence.
ACCESSION   DAORPG010000305 DAORPG010000000
VERSION     DAORPG010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5371_length_2338_cov_2.632939"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PLX85_07040"
     CDS             complement(<1..266)
                     /locus_tag="PLX85_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase"
                     /protein_id="HRC62961.1"
                     /translation="MRAFDLQAALRPALRGMTGYSPIAPPEEVAARYGIPVERIVKLD
                     ANENPYGPSPRAVEAVRALTGTNRYPDPDQRAIRAALGARHGIDP"
     gene            complement(267..1604)
                     /gene="hisD"
                     /locus_tag="PLX85_07045"
     CDS             complement(267..1604)
                     /gene="hisD"
                     /locus_tag="PLX85_07045"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019010817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRC62962.1"
                     /translation="MTAHSLRIVEGAGAARQSVLNRGATADATLPDAVQRSIVETFGE
                     PLTAAQVVARIIEDVRREGDAAVKRYSQRFDGSADAPMEVTSEEIDAAFSMVAPELLE
                     AVEFMADRVRAFHVHQRQHGPQPFTEHGLGMIVRPIQRAGIYMTGNVAVLPSSVVHTA
                     VPAVAAGVEEIVGVTAALPDGSVHPLKLVASRLAGVQRVFRASGAQALAALAFGTESI
                     PRVEKVFGPGNIFVTLAKQQLYGQVGIDALYGPTETVVLADDSASPDLCAADLLAQAE
                     HDALARPVLITTSVEHARAVAEEVEAQLADLERGAIARAAIERGGAVVAADLDEAIAL
                     ANEFAPEHLCLVTRDPEAVAARIQNAGGLFIGESSPEVLGDYVAGPSHVMPTGGSARF
                     ASPLTVNDFLKTISTIALSEDDLRRIGPYAAALARAEGLTGHARSIELRLEGR"
     gene            complement(1601..>2338)
                     /locus_tag="PLX85_07050"
     CDS             complement(1601..>2338)
                     /locus_tag="PLX85_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HRC62963.1"
                     /translation="SHLASARVLAGRLAHPGIEADRGAYYLGATAPDIRVLTRLDREH
                     THFFTLDDFEDQDSVARLFAEHPHLRDAVVLDATTRAFMAGYLTHLLMDQRYIERIYR
                     EFFGASSAYQGDPRGNILDRLLQYEMDRREREDVDAMAEIREALTGTSAAADIGFIER
                     ATLQQWREVACDIAGQPPTWDRFQRIASRHLPAAGIDTEEALEQFYRDLPDLLKETID
                     HVTEERMREFIDLTVDDATDRLKEYLQ"
BASE COUNT          338 a          700 c          958 g          342 t
ORIGIN      
        1 gggtcgatgc cgtggcgggc cccgagggcg gcgcggatgg cacgctggtc ggggtccggg
       61 tagcggttgg tgccagtgag ggcacgcacc gcctcgacgg cccggggcga ggggccgtag
      121 gggttttcat tggcgtcgag cttcacgatg cgctcgaccg ggatgccgta gcgggcggcg
      181 acctcctcgg gcggggcgat cgggctgtag cccgtcatgc cgcgaagggc cgggcggagg
      241 gcggcctgga ggtcgaaagc gcgcatctag cggccctcga gacggagttc gatggagcga
      301 gcgtggccgg tgaggccctc ggcgcgggcg agcgcggcgg cgtaggggcc gatacggcgt
      361 agatcgtcct cggagagcgc gatcgttgag atcgtcttga gaaagtcgtt cacggtgagg
      421 ggcgaggcaa agcgcgccga gccgcccgtc ggcatgacgt ggctaggccc ggctacgtag
      481 tcgccgagca cctcggggga ggactcgccg atgaagaggc cgcccgcgtt ctggatgcgc
      541 gcggcgaccg cctcggggtc gcgggtcacg aggcagaggt gctcgggggc aaactcgttg
      601 gccagcgcga tcgcttcgtc gaggtccgcg gccacgacgg cgccaccgcg ctcgatggcg
      661 gcacgggcga tggcgccacg ctcgaggtcg gcgagctggg cctcgacctc ttcggcgacc
      721 gcgcgggcgt gttcgacgct cgtggtgatg aggacggggc gggcgagcgc gtcgtgttcg
      781 gcctgtgcga ggaggtcggc ggcgcagagg tccggcgagg ctgagtcgtc ggcgaggacg
      841 accgtctcgg tggggccata aagcgcgtcg atgccgacct gcccgtagag ctgctgcttg
      901 gcgagcgtga cgaagatgtt gccggggccg aagaccttct cgacgcgcgg gatcgactcg
      961 gtgccgaagg cgagggcggc cagcgcctgt gcacccgagg cgcggaagac gcgctgcacg
     1021 cctgccaggc gggaggcgac gagcttcagc gggtgcactg agccgtccgg cagggcggcg
     1081 gtgacgccca cgatctcctc gacgcccgcg gcgacggctg ggacggccgt gtgcacgacg
     1141 gacgacggca gcacagccac gttgcccgtc atgtagatgc cggccctctg gatggggcgg
     1201 acgatcatgc cgagcccgtg ctcggtgaag ggctgggggc cgtgctggcg ctggtggaca
     1261 tggaaggcgc gcacacggtc cgccatgaac tcgacggcct cgaggagctc gggggcgacc
     1321 atcgagaagg cggcatcaat ctcctcggaa gtgacttcca tgggggcgtc cgcggagccg
     1381 tcgaagcgct gggagtagcg cttcacggcg gcgtccccct cgcgacggac gtcctcgata
     1441 atgcgggcga cgacctgggc ggcggtgagt ggctcgccaa aggtctcgac aatggagcgc
     1501 tgaacggcgt cggggagggt ggcgtcggca gtggccccgc gattgaggac cgactggcgg
     1561 gcggccccgg cgccctcgac gatccgcagg gagtgggccg tcactggagg tactccttca
     1621 gccggtcggt ggcgtcatcc accgtgaggt cgatgaactc gcgcatgcgc tcctcggtca
     1681 cgtggtcgat ggtttccttc aggaggtcgg gcaggtcgcg gtagaactgc tcgagggcct
     1741 cctcggtgtc gatgccggcg gccgggaggt ggcggctggc gatgcgctga aaacggtccc
     1801 aggttggcgg ctggccagcg atgtcgcagg cgacctcgcg ccattgctgc agggtggcgc
     1861 gctcgatgaa gccgatgtcg gccgcggcgg aagtgccggt cagggcctcg cggatctcgg
     1921 ccatggcgtc gacgtcctcg cgctcccgtc ggtccatctc gtattgcagc agacggtcga
     1981 ggatgttgcc gcgcgggtcg ccctggtagg ccgaggacgc gccgaagaac tcacggtaga
     2041 tgcgctcgat gtaacgctgg tccatgagga ggtgcgtcag gtagcccgcc atgaaggcgc
     2101 gcgtcgtggc atcgaggacg acggcgtcgc gaaggtgcgg gtgctcggcg aagaggcgag
     2161 cgaccgagtc ctgatcctcg aaatcgtcga gcgtgaagaa gtgcgtgtgc tcgcggtcga
     2221 gacgcgtgag cacgcggatg tcgggcgcgg tcgcgccgag gtaataggcg ccacggtcgg
     2281 cctcgatgcc ggggtgcgcg aggcggcccg cgaggacgcg ggcgctggcg aggtgcga
//
LOCUS       DAORPG010000306         2337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5374_length_2337_cov_3.332603, whole genome shotgun sequence.
ACCESSION   DAORPG010000306 DAORPG010000000
VERSION     DAORPG010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5374_length_2337_cov_3.332603"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..716)
                     /locus_tag="PLX85_07055"
     CDS             complement(69..716)
                     /locus_tag="PLX85_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HRC62964.1"
                     /translation="MNDEAPRERASIIMLSDVVCPWCYVGLMELEKLQENFPIDVHWS
                     PFLLDPTVPPEGRPQTPYNKPGDPPTPVEERAERLGIHFTRGRTFRPNSHLALEAAMY
                     ASEAGIDDKPLHRALYKAHFDDLEDVSDVDVLVRIGAENGLNADELRDVLETRRYQEA
                     VDDAIGWAQSIGVTAVPTFIIDERHGVVGAQEYEVLERVMLQLGYEKHPPVAADA"
     gene            complement(794..1285)
                     /locus_tag="PLX85_07060"
     CDS             complement(794..1285)
                     /locus_tag="PLX85_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62965.1"
                     /translation="MTDSEARESRQLRLALLALAGLGLAGTAVELVLERHWDGLEQLI
                     PWPFLAAGVVSLVLLSIRPGAASVRVARVLAAALFIGASVGIWRHVAANYDAGFLDFR
                     YADRWPSMSGLDRWWHAATKSVGPAPVLAPGVLAQVGLALLFATVRHPALRSHAEEKS
                     TIG"
     gene            complement(1282..1719)
                     /locus_tag="PLX85_07065"
     CDS             complement(1282..1719)
                     /locus_tag="PLX85_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62966.1"
                     /translation="MNPRYVLVSVGLGAALLAAACSSSSDKSTPTAAAIATTAASTQT
                     AAASTATASTTNAIATTTAGTTATAAAAAKKVSANNATIAEITAALEAAGVSNASRWA
                     REVDEYRPYSTTDTNLNKLRQELAKYNPGPGVVDAIVATLQLP"
     gene            1837..>2337
                     /locus_tag="PLX85_07070"
     CDS             1837..>2337
                     /locus_tag="PLX85_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRC62967.1"
                     /translation="MEVGLMVEGQNGLSWERWRHILALAERLGFPSLHRSDHYFIGTQ
                     QSSIEAYLSLAVAAVETSRIRFGPLVSPVTFRHPVDVARMGAQLDVLSGGRFKLGLGA
                     GWNEPEHVAYGLPFPGAKERLDRLEEALRLMRALWDEGEATFEGRYYSLSGATMLPRA
                     TRGRPWV"
BASE COUNT          333 a          757 c          869 g          378 t
ORIGIN      
        1 gcgacccccg gggcgctgcg cttctctctg tacccggtca gcgggtcagt tcgccggggt
       61 ggaccgagct aggcgtcggc ggcgacgggc gggtgcttct cgtagcccag ctggagcatg
      121 acgcgctcga ggacttcgta ctcctgggcg ccgacgacgc cgtggcgctc gtcgatgatg
      181 aacgtgggca cggcggtgac gccgatgctc tgggcccagc caatcgcgtc gtccacagcc
      241 tcctggtagc gccgcgtctc gaggacgtcg cgcagctcgt ccgcgttcag cccgttctcc
      301 gctccgatgc ggaccagcac atcgacgtcg ctcacgtcct cgaggtcgtc gaagtgcgcc
      361 ttgtacagcg cgcggtgcag cggcttgtcg tcaatgccgg cctccgacgc gtacatcgcc
      421 gcctcgagcg cgaggtgcga gttcgggcgg aaggtgcggc cacgggtgaa gtggatgccc
      481 agccgctcgg cgcgctcctc gacgggggtc ggcgggtcgc cgggcttgtt gtacggcgtc
      541 tgcggccggc cttccggcgg cacggtcgga tcgaggagga agggcgacca gtgcacgtcg
      601 atcgggaagt tctcctggag cttctccagc tccatcaggc cgacatagca ccacggacag
      661 acaacgtccg agagcatgat gatggacgct cgttcgcgcg gtgcctcgtc gttcatggcg
      721 gccctcccgg ctgtgattcg tccgcccatc gtaccgcggg ccgcgacgcc tcgggccatt
      781 cgaggagacg gtcctagccg atggtggact tctcctcggc gtgagaacgc agggcggggt
      841 gacggacggt ggcaaagagg agcgcgagtc ccacctgggc gaggacgccc ggggcgagga
      901 caggtgccgg accgaccgac tttgtggcgg cgtgccacca gcggtcgagg ccggacatcg
      961 acggccagcg gtcggcgtag cggaagtcga ggaagcccgc atcgtagttc gcggccacgt
     1021 gccgccagat gcccacggag gcgccgatga agagcgcggc cgcgagcacc cgtgccaccc
     1081 ggacgctcgc ggcgcccggc cggatggaga gcaggacgag gctcacgacc cccgcggcga
     1141 ggaacggcca cgggatcagc tgctcaagcc catcccagtg acgctcgagg acgagttcga
     1201 cggccgtgcc ggcgaggccc agcccggcca gggcgagcag cgcgagccgg agttgcctcg
     1261 actcgcgcgc ctcggagtcg gtcacggcag ctgcagtgtg gcgacgatcg catcgacgac
     1321 gccggggccg ggattgtact tggccagctc ctgccgaagc ttgttgaggt tcgtgtcggt
     1381 cgtggagtac gggcggtact catcgacctc ccgtgcccag cggctcgcgt tgctgacgcc
     1441 tgccgcctcg agggccgcgg tgatttcggc gatcgtggcg ttgtttgcgg acaccttctt
     1501 cgccgccgcc gcggctgtgg ccgtcgtgcc ggcggtggtc gtcgcgatgg cgttggtcgt
     1561 ggacgccgta gccgtcgagg cggctgccgt ctgcgtgctc gccgcagtgg tcgcgatcgc
     1621 ggcggcggtc ggcgtgctct tgtcgctgct actcgaacag gcggccgcga ggagggccgc
     1681 gccgaggccg acgctgacga ggacgtagcg agggttcatg ggactccctg catctggagc
     1741 atgcttcgat ctctatcaag ctcatggtag atgggcgatg ctaaccgcag ccgcgcaagg
     1801 tgttaacgaa gatcgaggcg ttcagaggag gctgccatgg aggtcgggtt gatggtcgag
     1861 ggccagaatg gcctgagctg ggaacggtgg cggcacatcc tcgcgctcgc cgagcgtctc
     1921 ggctttccca gcctgcaccg ctccgaccac tacttcatcg ggacgcagca atcctcgatc
     1981 gaggcgtacc tttcgctcgc ggtggccgcg gtcgagacga gccgaattcg cttcgggccg
     2041 ctcgtgtcgc ccgtgacgtt ccggcacccc gtcgacgtcg cgcgaatggg cgcgcagctc
     2101 gatgtgctca gcggcgggcg attcaagctc ggcctcggcg cgggctggaa cgagcccgaa
     2161 cacgtcgcgt acggcctgcc gtttccgggt gcaaaggagc gcctcgaccg cctggaggag
     2221 gcgctgcggc tgatgcgtgc gctgtgggac gagggcgagg cgaccttcga ggggcgctac
     2281 tactcgctga gtggggcaac gatgctgccg cgcgcgacgc gcggacggcc atgggtg
//
LOCUS       DAORPG010000307         2336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5379_length_2336_cov_3.261727, whole genome shotgun sequence.
ACCESSION   DAORPG010000307 DAORPG010000000
VERSION     DAORPG010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5379_length_2336_cov_3.261727"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLX85_07075"
     CDS             complement(<1..180)
                     /locus_tag="PLX85_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62968.1"
                     /translation="MPVIVVSGSESAAGVTTVAVGLAHRLAYAGHEVHLERLAFGANA
                     ERDAATFGSLDFAASS"
     gene            complement(243..>2336)
                     /locus_tag="PLX85_07080"
     CDS             complement(243..>2336)
                     /locus_tag="PLX85_07080"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HRC62969.1"
                     /translation="TGSGAQIVDSSGRRVDLDDAKVDPNDLTQMAVSVSRLAEGTYTV
                     AWHNVSAVDGHGLQGSFVFFVGSRPPDAPSSASTKPPLLQSRVDPAVRWFTLFGALVA
                     TGAILFDPLILRVAAGREPRRAVTGALETARGRIAIVARAGAFAFLGGTVGQLLLQAG
                     ITAGVPAYRVTPGDVADIFSTAWGLRWLVRVCCLVIVVVLMGIEWGVERQGRRPRYAS
                     LLWVPVSLLLVVAMAMVSLSSHGAATTDLEVPGAINDGLHLAASALWVGGLCALVLTV
                     RAFSVLRWEERRGVVAALVARFSLFAGLSVGVLLVTGLYSSWLQVTSRAAFDTPYGSV
                     LAVKLVLVAGLLTLGALNLLWVRPRLAQIRGETWLRRLVLGEVALAVLVVVTVAGLTS
                     LEPARQVHSRDEQQAALSFGETVQGTRIGGTVAPGTVGTNRVVVTVADRNGAPVNDAS
                     NVLVRLKFLDRDLSPIELTATSVGGGRYEVPPTVLSIVGAWQIEVEVSRPGAFDARTA
                     SRFLVTSPTDVSTTASSAAPPASDGRTYWSWVMIAAGALVLVLLPRAWTTRPARARSR
                     VLGTAVVLAGVVLFYGGHTHAPAPQSVLNQTNPYPPDSRSIEAGRSLYEQQCVKCHGA
                     AGHGDGPEASGLVPPPADLSTHVPLHTDGTVFYFISSGFPGTAMPAFAGTLSEQQRWD
                     LVNYLRTLAQPLPSR"
BASE COUNT          414 a          855 c          771 g          296 t
ORIGIN      
        1 ggaactcgcg gcgaagtcga gcgatccaaa ggtggcggcg tcccgttccg cgttcgcgcc
       61 aaaggcgagc cgctcgaggt gcacctcgtg ccccgcgtac gccagacgat gagcgagacc
      121 gaccgcgacg gtggttaccc ccgccgcgga ctcgctgccg ctcacgacga tgaccggcat
      181 cgccagctcc ttccctccgt cggccagaac gtactacggc ccgtctcctc gcgcgagcaa
      241 cgctaccgac tgggcaacgg ctgcgccagc gtccgcaggt agttcacgag gtcccagcgt
      301 tgctgctcgg aaagggtgcc tgcgaacgcg ggcatcgccg tgccggggaa cccgctggag
      361 atgaagtaga acacggtgcc atccgtgtgc agcgggacat gcgtgctgag gtctgccggc
      421 ggagggacga gtcccgacgc ctccggtccg tcgccgtgcc cggctgcccc gtggcatttc
      481 acacactgct gctcatacag cgagcgcccg gcctcgatgg atcggctgtc tggcggatat
      541 ggattcgttt ggttgagcac cgactggggc gccggagcgt gcgtgtggcc gccgtagaac
      601 agcacgacgc ccgcgagcac gaccgccgtg ccgaggacgc gcgaccgcgc tcgcgctggc
      661 cgcgtcgtcc atgcccgggg caacaacacc agcaccagcg cgccggcggc gatcatcacc
      721 cacgaccagt acgttcgccc atcggatgcc gggggcgccg ccgagctggc cgtcgtgctg
      781 acgtcggtcg gcgacgtcac gaggaaccgc gaggccgtcc gtgcatcgaa cgccccgggc
      841 cgagacactt cgacctcgat ctgccatgca ccaacgatgc tgaggacggt cggcggtacc
      901 tcgtatcgac cgccacccac cgaggtcgcc gtcaactcaa tcgggctcag gtcgcggtcg
      961 aggaacttca accgtaccag cacgttcgag gcgtcgttca caggcgcgcc gttccgatct
     1021 gccacggtca cgacgacgcg attcgtgccc acggtgccgg gtgccaccgt ccccccgata
     1081 cgcgtgccct gcaccgtctc cccgaacgaa agggcggcct gctgctcgtc gcgggaatgc
     1141 acctggcggg ctggctcgag actggtgagc ccggcgacgg tcaccaccac gagcaccgcc
     1201 agcgcgacct caccgaggac gaggcggcgc agccacgtct cgccgcggat ctgcgccagt
     1261 cgcggccgca cccagagcag attgagcgcc ccgagcgtca gcagtcccgc gacgagcacg
     1321 agtttgaccg cgagcactga gccgtacggc gtatcgaagg ccgccctcga ggtgacctgc
     1381 aaccacgatg agtacagccc ggtcacgagc agcaccccga cgctcaaccc ggcgaacagc
     1441 gagaagcgcg ccacgagggc agccaccact cctcgccgct cctcccagcg gagtaccgag
     1501 aaggcccgga cagtgagcac cagtgcacag agaccgccga cccagagcgc cgaagcggcc
     1561 aggtgcagcc catcgttgat cgcgccgggc acctcgaggt cggtcgtggc cgcaccgtgg
     1621 ctcgagaggc tcaccatggc catcgcgacc acaagaagga gcgagacggg gacccacagc
     1681 agcgatgcgt aacgcggcct ccgcccctgc cgttccacgc cccactcaat acccatcaac
     1741 acgacgacga tgacgaggca gcagacgcgc accagccagc gcaggcccca cgccgtggag
     1801 aaaatgtcag caacgtcgcc gggcgtgact cgataggcgg ggacgccggc cgtgatgccc
     1861 gcctgcaaga ggagctgccc gaccgtccct ccgaggaacg caaaggctcc cgcgcgcgcg
     1921 acgatcgcga tcctccctcg cgcagtctcg agggctcccg tgaccgcgcg gcgtggctcg
     1981 cggccggccg cgacgcgcag gatcaacggg tcgaacaaga tcgcgccggt cgcgaccagc
     2041 gccccgaaca gcgtgaacca gcgcaccgcc ggatccacgc gcgactggag cagcggcggc
     2101 ttggtggagg cgctcgaagg cgcatcaggc gggcggctgc ccacgaagaa gacgaacgac
     2161 ccctggagcc cgtgcccgtc gacggccgag acgttgtgcc acgcgaccgt ataggtcccc
     2221 tccgccagcc tggagacgga gaccgccatt tgtgtcaggt cgttcgggtc caccttcgcg
     2281 tcatcgaggt ccacccggcg gcccgacgag tcgacgatct gggcgccgct tcccgt
//
LOCUS       DAORPG010000308         2327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5420_length_2327_cov_3.667694, whole genome shotgun sequence.
ACCESSION   DAORPG010000308 DAORPG010000000
VERSION     DAORPG010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5420_length_2327_cov_3.667694"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLX85_07085"
     CDS             complement(<1..397)
                     /locus_tag="PLX85_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRC62970.1"
                     /translation="MSDLDTALAIAVEAHRGQVDKAGQPYILHPLRVMARVSTPAERL
                     VAVLHDVVEDSPTTLDDLRAAGFPEEVVRAVDFLTRREDETYEAFIERVTGDPLARRV
                     KLADLEDNMTITRLNELDDRAVERLGRYLR"
     gene            complement(411..632)
                     /locus_tag="PLX85_07090"
     CDS             complement(411..632)
                     /locus_tag="PLX85_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62971.1"
                     /translation="MGTKELQALIEVLQDQVAQGREGHVIGTWTIHYDKQRSAFTFDK
                     CEDGVYCEERPAVIALDHTVLDRGGPIGG"
     gene            747..1820
                     /locus_tag="PLX85_07095"
     CDS             747..1820
                     /locus_tag="PLX85_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRC62972.1"
                     /translation="MGDKASIGNYFEDLTAGLEIEHAVPRTLTEGDAAMYIALTADRY
                     PLHCDAEFARSLGFQRETVNDLLVFHTVFGKSVPDISLNAVANLGYADVRFGVPAYPG
                     DTLRAHTTVLGQRESASGGTGVVWVRTVGRNQHGEEVLSFIRWVLVNRRQPDVSTGVD
                     DAPKTPASVAPEDFVIPAQLDLSRFDPIVTGGRAWWGDYEPGERIHHVEGVAIEEAEH
                     QMAARLYQNTARAHFNAHALKDARFGKRVIYGGHIISMARALSFNGLENAVRILAWNG
                     GTHSNPTFAGDTVFAWTDVLERIDLGRPDAGALRLRLVAVKNCDPATEEQPFKVADDN
                     GRERYHPNVVLDLDYTVLMPKAR"
     gene            1915..>2327
                     /locus_tag="PLX85_07100"
     CDS             1915..>2327
                     /locus_tag="PLX85_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62973.1"
                     /translation="MSTDVQNARLLVDSAQRVVDAALAHAAATTEGGAKIDDHQVHTE
                     RVTYLATQVRAARELTSAAERLEAAGRADSLMAAEAFVYAAEATHALGSAIDAAWEDF
                     GVGEAVAAIQAQAVRDAMRAGLSEARIREIGRARIA"
BASE COUNT          351 a          792 c          855 g          329 t
ORIGIN      
        1 ctcgcaggta gcggcccagg cgctcgacgg cgcggtcgtc cagctcattc aggcgggtga
       61 tcgtcatgtt gtcctcgagg tccgcgagct tcacgcggcg cgcgagcggg tcgcccgtca
      121 cgcgctcgat gaaggcctcg taggtctcgt cctcgcggcg ggtgaggaag tcgaccgcgc
      181 gcacgacctc ctccgggaag cccgcggcgc ggaggtcatc gagggtggtc gggctgtcct
      241 cgacgacgtc gtgcagcacg gcgaccaggc gctcggcggg cgtcgagaca cgcgccatca
      301 cgcgcagggg atgcaggatg taaggctggc ccgccttgtc gacctggccc cgatgggcct
      361 cgacggcaat ggcgagcgcg gtgtcgagat cgctcacggg gcgggtggcg tcagccgccg
      421 atgggtccgc ccctgtcgag cacggtgtgg tcgagggcga tgacggcagg gcgctcctcg
      481 cagtagacgc cgtcctcgca cttgtcgaag gtgaaggcgg agcgctgctt gtcgtagtgg
      541 atggtccagg tgccgatgac gtggccctcg cggccctgcg ccacctgatc ctgcagtacc
      601 tcgatcagcg cctgtagttc cttcgtgccc acgcgtggcc ctccttcggc tcgatcccga
      661 gtataggggc gagcaggctc gctggcgggc gcgcgcctcc cctcttgggg ccccgctccc
      721 tacaatggca ccgcgaggag ggcagcgtgg gcgacaaagc cagcattggc aactacttcg
      781 aggacctcac ggccggactc gagatcgagc atgcggtccc gcgcaccctc acggagggcg
      841 acgccgcgat gtacatcgcg ctgacggcgg accgctaccc gctgcactgc gacgcggagt
      901 tcgcgcggag cctcggcttc cagcgcgaga cggtgaatga cctgctggtg ttccacacgg
      961 tcttcgggaa gtccgtgccg gacatctcgc tgaacgcggt cgcgaacctc gggtacgcgg
     1021 acgtacgctt cggcgtgccg gcgtacccgg gcgacacgct gcgagcgcac acgaccgtcc
     1081 tcggccagcg cgagtcggcc tcgggcggca cgggcgtcgt ctgggtccgc acggtgggcc
     1141 gcaaccagca cggcgaggaa gtgctgtcct tcatccgctg ggtgctggtg aaccgacggc
     1201 agccggacgt gagcacgggc gtggacgacg ctccgaagac gcccgcctcg gtcgcccccg
     1261 aggacttcgt catccctgcg caactcgacc tatcgaggtt cgacccgatc gtgacgggcg
     1321 gccgcgcgtg gtggggcgac tacgagcccg gcgagcgcat tcaccatgtc gagggcgtgg
     1381 cgatcgagga ggccgagcac cagatggccg cccggctgta ccagaacacg gcgcgtgccc
     1441 acttcaacgc gcatgcgctc aaggacgcgc gctttgggaa gcgggtcatc tacgggggcc
     1501 acatcatctc gatggcgcgg gcgctgtcgt tcaacgggct cgagaacgcg gtgcgcatcc
     1561 tcgcctggaa cggggggacg cacagcaacc cgacgttcgc gggggacacg gtcttcgcct
     1621 ggacggacgt actcgagcgc atcgacctcg gccggccgga cgcgggcgcg ctgcggctgc
     1681 ggctggtggc agtgaagaac tgcgacccgg cgaccgagga gcagccgttc aaggtcgcgg
     1741 acgacaacgg acgggagcgc taccacccga acgttgtgct cgacctcgac tacacggtcc
     1801 tcatgccgaa ggcgcgctga gtgcgcttcc gggcgcggtc ctcgttcgtc tcgctacctc
     1861 gcgcgtaggg acgccgcgcc ggcggccacg cgcgtatcag gaaggaacgc cgacatgtcc
     1921 accgacgtcc agaacgcgcg gctcctcgtc gacagcgccc agcgcgtggt cgacgccgcg
     1981 ctcgcccacg ccgcagccac gaccgaaggc ggcgcgaaga tcgacgacca ccaggtccac
     2041 accgagcgcg tgacctacct cgcaacgcag gtgcgggcgg cgcgcgagct gacctcggcg
     2101 gcggaacgcc tcgaggccgc gggcagggcc gactcgctca tggcggcgga ggcattcgtg
     2161 tacgcggccg aggcgacgca cgcccttgga agcgccatcg acgcggcctg ggaggacttc
     2221 ggcgtcggtg aggcggtggc ggcgatccag gcgcaggcgg tgcgcgacgc gatgcgcgcg
     2281 gggctgtcgg aggcgcgcat ccgcgagatt gggcgcgcgc ggatcgc
//
LOCUS       DAORPG010000309         2312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5487_length_2312_cov_2.558263, whole genome shotgun sequence.
ACCESSION   DAORPG010000309 DAORPG010000000
VERSION     DAORPG010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5487_length_2312_cov_2.558263"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="PLX85_07105"
     CDS             <1..626
                     /locus_tag="PLX85_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC62974.1"
                     /translation="PPQALPHLFQLGVPSEEIDLVLLSHAHSDHIGGVDLFLLDAVYR
                     TWPRRTRPLAVGGPPGIYDRLREVIGESERLPPRDDPRITWFEGGDGASFEWADATVE
                     CFEVDHDPALTSLAFRVRAGDSVVAYSGDTRPCAAVERLAEGADLLVLECGVGTYHFD
                     WPDVLALRSRLPASTQVLVTHYPDEGSDAVRGVPGLRLAEDFGTYEA"
     gene            772..1332
                     /locus_tag="PLX85_07110"
     CDS             772..1332
                     /locus_tag="PLX85_07110"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HRC62975.1"
                     /translation="MAYGAGIARGLLVTFKHIFKPPVTVNYPEETRQVPVRARTNLLW
                     FEERCTGCSTCAQACPDGCILVQTSPRGDGTLNIDRYEIDFRICMYCGLCTEACPYQA
                     IQAGGRYNDAVYVFENMYRNREALTREAKAYMEQTGGRYPNGQLQELAPPRTAMPTAR
                     SRVQTAGSDGPYGPQHLPSRQDRNGS"
     gene            1375..>2312
                     /locus_tag="PLX85_07115"
     CDS             1375..>2312
                     /locus_tag="PLX85_07115"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRC62976.1"
                     /translation="MAKITINGTEVEAEAGRRLVEVIKEQGIRITNLCYIDGLEPYAG
                     CRTCLVDIEGGRPTSIQLSCTAQVAEGMVVRTDTPEVKRARQGVMSLIMANHPDRCLT
                     CHRRVHCQPGEICLRDDVVTHRCLTCSKNYRCELQTSTEMIDMGEYEEPWVGEVRSYY
                     ETPPPEPDRANPYLEFDPQMCIICTRCVRACADIRHTGAITLSGKGSSTAIAFGTGGP
                     VHESNCDFCGACIDVCPTATLMEKPNKWVGLAEEWTNSTCNSCSVGCTISYGVANDRP
                     VIVRPDRLNQFSRDQICVRGRFHYDAVAENQRLSRSL"
BASE COUNT          377 a          804 c          794 g          337 t
ORIGIN      
        1 gcccgccgca ggcgctgccg cacctcttcc agctcggcgt gccgtccgag gagatcgacc
       61 tcgtgctgct gtcccacgcg cacagcgacc acatcggcgg ggtcgacctc ttcctcctcg
      121 acgccgtcta ccgcacctgg ccgcggcgca cgcggccgct cgccgtcggc gggccgcccg
      181 gcatctacga ccggctgcgc gaggtgatcg gagagtcgga gcgactgccg cctcgcgacg
      241 acccacgcat cacctggttc gagggcggtg acggcgcctc attcgagtgg gcggacgcga
      301 cggtcgagtg cttcgaggtg gaccacgacc cggcgctcac ctcgctggcg ttccgggtgc
      361 gggcgggcga ctcggtggtc gcgtacagcg gcgacacgag gccatgtgcc gcggtggagc
      421 gactggccga aggcgcggac ctgctcgtgc tcgagtgcgg cgtgggcacc tatcacttcg
      481 actggcccga cgtgctggcg ctccggtcgc ggctgccagc gagcacgcag gtcctcgtga
      541 cgcactaccc ggacgaaggc tcggacgcgg tgcgaggcgt gccggggttg cggctggccg
      601 aggacttcgg gacgtacgag gcgtgagcgt gggattttcg taaccgctgc ttcgcgaacg
      661 gcggctgaac ccctcgttcg tgctacgatt cacacggcgt ggcgggggtg tcccttatct
      721 cctcgtccgc tatgctccgc gacgcacacg ttcgggggag acgaggctgc cgtggcgtac
      781 ggtgcgggca tcgcccgagg cctgctggtc accttcaagc acattttcaa gccccctgtc
      841 accgtcaact atcccgagga gacgcgacag gtgccggtgc gcgcgcgcac gaacctactc
      901 tggttcgagg agcgctgcac gggctgctcc acgtgcgcgc aagcgtgccc ggacggatgc
      961 atcctcgtgc agacctcgcc acgcggtgac ggcacgctga acatcgaccg ctacgagatc
     1021 gacttccgca tctgcatgta ctgcgggctg tgcaccgagg cgtgcccgta ccaggcgatc
     1081 caggcgggcg ggcgctacaa cgacgccgtg tatgtcttcg agaacatgta ccgcaaccgc
     1141 gaggcgctga cgcgcgaggc gaaggcgtac atggagcaga ccggcgggcg ctacccgaac
     1201 gggcagctgc aggagctggc cccgcctcgg acggcgatgc cgacggcacg cagccgcgtg
     1261 cagacggcgg ggagcgacgg gccgtacggc ccccagcacc tgccgagccg gcaagaccgc
     1321 aacggcagct gacgcgagcc tcggcacgag ggcgaagaga aagagcggga tcgcgtggcc
     1381 aagatcacca tcaatggcac ggaagtcgag gcggaggccg gacgccggct ggtcgaggtg
     1441 atcaaggagc aggggatccg gatcacgaac ctctgctaca tcgacggcct cgaaccgtat
     1501 gccggctgcc gcacctgcct cgtcgacatc gagggcgggc gcccgacgag catccagctc
     1561 tcctgcaccg cgcaggtcgc cgaggggatg gtggtgcgca ccgacactcc cgaggtgaag
     1621 cgcgcgcgcc agggcgtgat gtcgctgatc atggcgaacc accccgaccg ctgcctcacc
     1681 tgtcaccgcc gcgtgcactg ccagcccggc gagatctgcc tgcgcgatga cgtggtcacg
     1741 caccgctgcc tgacctgctc gaagaactac cgctgcgagc tccagacctc gactgagatg
     1801 atcgacatgg gcgagtacga ggagccctgg gtcggcgagg tgcgctcgta ctacgagacc
     1861 ccgccccccg agcccgaccg cgcgaacccc tacctcgagt tcgacccgca gatgtgcatc
     1921 atctgcacgc gctgcgtgcg cgcctgcgcg gacatccggc acacgggcgc gatcacgctg
     1981 tcgggcaagg gttcgtcgac cgcgatcgcg ttcggcaccg gcggccccgt acatgagtcg
     2041 aactgcgact tctgcggcgc ctgcatcgat gtctgcccca cggcgaccct catggagaag
     2101 ccgaacaagt gggtggggct cgccgaggag tggaccaact caacctgcaa ctcgtgctcc
     2161 gtcggctgca cgatctcgta cggcgtcgcc aatgaccgcc cggtgatcgt gcgacccgac
     2221 cggctgaacc agttcagccg cgaccagatt tgcgtacggg gccgcttcca ctacgacgcc
     2281 gtcgcggaga accagcgcct cagccggtcg ct
//
LOCUS       DAORPG010000310         2308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5506_length_2308_cov_3.820684, whole genome shotgun sequence.
ACCESSION   DAORPG010000310 DAORPG010000000
VERSION     DAORPG010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5506_length_2308_cov_3.820684"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="PLX85_07120"
     CDS             <1..850
                     /locus_tag="PLX85_07120"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRC62977.1"
                     /translation="RRPDDAVLLVRPAAGLGVGAWSLPMAQMHEHATAEATASQVVRD
                     GLRMDPGWFQFAETLTFERGGFDIVINVFDAIGWSGEPRYAERDYEDAAWVRPDALED
                     VDAVPEVAAWLRGEDAPVPDDVVPERLASMLVEARADLLRTYSAIPDRDRTRPLDGDW
                     APVDVLAHLASAEAYYLREARQLASPEAHHWRPFNAEQWDADRAYRARPMNSEVTARL
                     DQVQTDSLKTIGGMVEAELAHYGSRGGGGALRVGEAIAIIAAHDREHAGQLEKMHSHA
                     RSGGGA"
     gene            850..1443
                     /locus_tag="PLX85_07125"
     CDS             850..1443
                     /locus_tag="PLX85_07125"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRC62978.1"
                     /translation="MLLLIDNYDSFTYNLYQYLAELGADVRVHRNDEVSVEDCLAMRP
                     ERIVISPGPCTPDEAGISVDLIKAAAGQVPLLGVCLGHQAIGQAFGADVVSAGEIMHG
                     KTSMVTHDGKGVFAGLPNPFEAIRYHSLAIAPDSVPEELEVSATSESGVIMGIRHRTM
                     LVEGVQFHPESIMTKVGHDLLRNFLEMRMPAPAGAAS"
     gene            1440..>2308
                     /gene="trpD"
                     /locus_tag="PLX85_07130"
     CDS             1440..>2308
                     /gene="trpD"
                     /locus_tag="PLX85_07130"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRC62979.1"
                     /translation="MIREALAALVDERRDLTEHEAYEAMGDIMRAGLDLAEGEKATAS
                     QFGSFVTALRVKGETVDELTGMAKAMREAALRVDTTVAPLLDTCGTGGSNKKVFNAST
                     TAAFVAAAAGAKVAKHGNRAMTSQSGAADLLEALGAVVELTPPQVARCIEEVGIGFMF
                     APAFHPAMKFAGPLRREVGFRTVFNILGPLTNPAGAKCHVMGAASPELAERLAHVLAR
                     LNMRHAIVACGDDGLDDISVSGPTTVFEVRGGEVTQKTVIPFDLGLSIHPFEAIDGGS
                     PTEVFTAAAGLRT"
BASE COUNT          357 a          864 c          740 g          347 t
ORIGIN      
        1 ccgccggcca gacgacgccg tgctgctcgt gcggcccgcc gccggcctcg gggtgggcgc
       61 ctggtccctg cccatggccc agatgcacga gcacgcgacc gccgaagcca ccgcttcgca
      121 ggtcgtgcgt gacggactgc gcatggaccc gggctggttc cagttcgccg agacgctcac
      181 cttcgaacgt ggcggcttcg acatcgtgat taacgtcttc gatgccatcg gatggtccgg
      241 cgaaccccgc tacgccgaac gcgactacga ggatgccgcc tgggtgcgtc cggacgcact
      301 cgaggacgtg gacgccgtcc ccgaggtcgc cgcctggctc cggggtgagg atgccccggt
      361 ccccgacgac gtcgtccccg aacgtctcgc ctcgatgctc gtcgaggcgc gcgccgatct
      421 gctgcgcacc tactcggcga tccccgaccg cgaccgtacg cgcccgctcg acggcgactg
      481 ggcgcccgtc gatgtcctcg cgcaccttgc aagcgccgag gcgtactacc tgcgcgaggc
      541 tcgccagctc gcatcgccgg aggcgcacca ctggcggccg ttcaatgccg aacagtggga
      601 cgccgaccgg gcttatcgcg cccggccgat gaattccgag gtcacggcac gcctcgacca
      661 ggtccagacc gactcactca agaccatcgg cggcatggtc gaagccgagc tcgcgcatta
      721 cggcagccgc ggtggaggcg gcgcgctgcg cgtcggcgag gccatcgcca tcatcgcggc
      781 tcacgaccgc gagcacgccg gtcagctcga gaagatgcat tcgcacgccc gcagcggagg
      841 gggcgcctga tgttgctgct catcgacaac tatgattcgt tcacctacaa cctctaccag
      901 tacctcgcgg agcttggcgc cgacgtgcgc gtccaccgca acgacgaggt gagcgtcgaa
      961 gactgcctcg cgatgcggcc cgagcgcatc gtgatctcgc cgggcccctg cacacccgat
     1021 gaggcgggca tctcggtcga cctcatcaag gcggccgccg gccaggtccc gctcctcggc
     1081 gtctgcctcg gtcatcaggc gatcgggcag gccttcggcg ccgacgtcgt ctccgcgggc
     1141 gagatcatgc acggcaagac ctcgatggtc acccacgacg gcaagggtgt cttcgccggc
     1201 ctgcccaacc ccttcgaggc gattcgctat cattcgctcg cgatcgcgcc cgattcagtg
     1261 ccggaggagc tcgaagtgtc tgccacctcc gagagcggcg tgatcatggg catccgccac
     1321 cgcacgatgc tcgtcgaggg cgtgcagttt catccggagt cgatcatgac gaaggtcggt
     1381 cacgacctgc tgcgcaactt cctcgagatg cgcatgcccg cgcccgcggg agccgcctcg
     1441 tgatccgcga ggccctggcc gcgctcgtcg acgaacgccg cgacctcacc gaacacgagg
     1501 cctacgaggc catgggcgac atcatgcgcg ccggcctcga cctcgccgag ggcgagaagg
     1561 cgaccgcctc ccagttcggc tccttcgtga ccgcgctccg cgtgaagggc gagacggttg
     1621 atgagctgac cgggatggca aaggccatgc gcgaggccgc gctccgcgtc gataccaccg
     1681 tcgcgcccct gctcgacacc tgcggcaccg gcggctccaa caagaaggtg ttcaacgcct
     1741 cgaccacggc cgccttcgtg gcggccgcgg ccggcgcgaa ggtcgcgaag cacggcaacc
     1801 gcgccatgac ctcccagtcc ggcgccgccg acctcctcga ggcgctcggc gccgtcgtcg
     1861 agctcacccc gccacaggtc gcccgctgca tcgaggaggt gggcatcggc ttcatgttcg
     1921 cgccggcctt ccaccccgcc atgaagttcg ccggcccgct ccgccgcgag gtgggcttcc
     1981 gcaccgtctt taacatcctc ggcccgttga ccaacccggc gggcgcaaag tgccacgtca
     2041 tgggggccgc cagccccgaa ctcgcggaac gcctcgccca cgtcctcgcg aggctgaaca
     2101 tgcgccacgc gatcgtcgcc tgcggcgatg acggcctcga cgacatcagc gtgagcggcc
     2161 cgaccactgt ctttgaggtg cgcggcggcg aggtcaccca gaagaccgtc atcccattcg
     2221 atctcggtct ctcgatccac ccgttcgagg cgatcgacgg cggctcgccg accgaagtct
     2281 tcactgctgc cgccggtctt cgcaccaa
//
LOCUS       DAORPG010000311         2308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5508_length_2308_cov_3.261873, whole genome shotgun sequence.
ACCESSION   DAORPG010000311 DAORPG010000000
VERSION     DAORPG010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5508_length_2308_cov_3.261873"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..871
                     /locus_tag="PLX85_07135"
     CDS             212..871
                     /locus_tag="PLX85_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRC62980.1"
                     /translation="MDLHSHSADASDDAGGTVEGYLKWIVARRKRGFRIDGFVLTEHR
                     EFDPTVDYSALAEQYGVTVLRGAELETDIGHVLVYGVSPELAKRFDFSSVALPHRELF
                     EVARDTGGFAVGAHAGRPRIGLAHHVHERGLTPDGIEVIEQLNGGSSSEENGRALELA
                     EEYGFRCVGGSDAHFVSAIGRCLTAFRRPITSIQALVEELAGGDYFPVTVEQTQRVDA
                     G"
     gene            871..1329
                     /locus_tag="PLX85_07140"
     CDS             871..1329
                     /locus_tag="PLX85_07140"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HRC62981.1"
                     /translation="MDDADAEFEYDRDILGVEFEVGQATITKEHIARYCAAVGETNPL
                     YLDDAAAAEGPYGAIIAPPSMLSGLLMEPRPDPDVRFGNTSYAAGTKFEYYEPVRAGD
                     TIHVSALVKEVYAKTGRSGTMVFAVYQSRYRNQRDESVAFEEFARVFRQA"
     gene            1330..1764
                     /locus_tag="PLX85_07145"
     CDS             1330..1764
                     /locus_tag="PLX85_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRC62982.1"
                     /translation="MDQRYFEDVEPGDEIETPWDATPEAVAEFMATRRLASGLPEIED
                     GPERVRPGFERPIVPGSMSLAVLSRLVTDWMGSRGRLRSISASYRRPVYRDDQLRALA
                     LVTDTEQQAEGGGLVKLDVYIENERGERPLQGVAVVELPLRP"
     gene            1961..>2308
                     /locus_tag="PLX85_07150"
     CDS             1961..>2308
                     /locus_tag="PLX85_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC62983.1"
                     /translation="MSRMPGAYLEARTTEIRDAAMRVFVRKGINAATMQDIASEAGMS
                     AGAIYRYFESKDQLVQAVFEYCREENRALFEDGRPEGASLLEAFVGVGRTVWDEFGEP
                     GARDRFAVRLAATA"
BASE COUNT          390 a          701 c          855 g          362 t
ORIGIN      
        1 accgccgcgc tggagcgcct cgcgacgggg ccctggcgcg aggcgcgtgc cgggcggcgt
       61 tgacgctgtt cgagggcgct cgcgagaatg agaagcgcgc atcgcgcata gccacccgac
      121 gaccagcggg caggacacga tcatcagcga gacgaccgcc atcgacgcca ccaacccctc
      181 ttcagccgcc ccggatgcgg ggcgctggta tatggacctg cactcgcatt ccgccgacgc
      241 ctcggatgac gccgggggga cggtcgaggg gtacctcaaa tggatcgtgg cgcgccgcaa
      301 gcgtggcttc cgcatcgatg ggttcgtgct cacggagcac cgcgagttcg acccgacggt
      361 ggattactcg gcgctggcgg agcagtacgg cgtgacggtc ttgcggggcg cggagctgga
      421 gaccgacatc gggcacgtgc tcgtgtatgg agtcagtccg gagctggcga agcggttcga
      481 cttctcctcg gtggcgttgc cgcaccgcga gttgttcgag gtggcgcgtg acacgggtgg
      541 attcgcggtg ggcgcgcacg ccgggcggcc gcgtatcggc ctcgcgcacc acgtgcacga
      601 gcgcggcctg acgccggacg gcattgaggt gatcgagcag ttaaacggcg gaagctcctc
      661 ggaagaaaac gggcgggcgc tcgagctggc ggaggaatac ggattccgct gcgtcggggg
      721 gtccgacgcg cacttcgtga gcgcgatcgg gcgctgcctg acggcgttcc gccggccgat
      781 cacctcgatc caggcgctcg tggaggagct ggccgggggc gattacttcc cggtgactgt
      841 cgagcagacg caacgcgtgg acgcggggta gtggacgacg cggatgccga gttcgagtac
      901 gaccgggaca tcctcggcgt cgagttcgag gtcgggcagg cgacgatcac aaaggagcac
      961 atcgcccgct actgcgccgc ggtgggcgag accaatccgc tctacctcga tgacgcggct
     1021 gcggcggagg ggccgtacgg ggcaatcatc gcgccaccga gcatgctgag cgggctcctg
     1081 atggagccgc ggcccgaccc cgacgtgcgg ttcggaaaca cctcgtatgc ggccgggacg
     1141 aagttcgaat actacgagcc ggtgcgagca ggggacacga tccatgtgtc agcgctcgtg
     1201 aaagaggtgt acgcgaagac cggccgctcc ggcacgatgg tgttcgccgt gtaccagtcc
     1261 cgctatcgca atcagcggga cgagtcggta gcgttcgagg agttcgcgcg ggtcttccgg
     1321 caggcgtaga tggatcagcg gtacttcgag gacgtcgagc ccggcgatga gatcgagacg
     1381 ccatgggatg cgacgccgga agcggtggcg gagttcatgg ccacccgccg cctcgcctcc
     1441 gggttgccgg agatcgagga tgggcccgag cgggtgcgac cgggcttcga gcggccgatc
     1501 gtcccgggtt cgatgagtct cgcggtgctg agccgcctcg tgaccgattg gatgggatcg
     1561 cgcggacggc tgcgcagcat ctcggcgagc taccggcggc ctgtataccg cgacgaccaa
     1621 ctacgcgcgc tcgctctggt gacggacacc gagcagcaag cggagggcgg cggcctcgtg
     1681 aagctcgacg tgtacatcga gaacgagcgc ggcgagcggc cgctccaggg tgtcgccgtg
     1741 gtggagctgc cgctgcgccc gtagctaccg acgttgttcg cttcgtggga ggcgtcctga
     1801 ccgcctcttg agatggtcga tcccgacgcc gttcgcgcgt cgttcccatg acggctccac
     1861 aagactgaca cttgaccgtt gacaatcgca atgtgaatga acaatcattc accgtcatga
     1921 atgaacgttc atttatcgat cgtgagatgc gagggacggc atgagccgga tgccgggcgc
     1981 atacctcgag gcgcggacga ccgagatccg ggacgccgcg atgcgtgtgt tcgtgcgcaa
     2041 ggggatcaac gccgccacga tgcaggacat cgcttccgag gccggcatga gcgcgggggc
     2101 gatctaccgc tacttcgaga gcaaggatca gctcgtacag gcggtcttcg agtactgccg
     2161 tgaggagaac cgcgcgctct tcgaggatgg acgcccggaa ggagcgtcgc tgctcgaggc
     2221 gttcgtcgga gtgggccgca cagtctggga cgagttcggg gagccgggag cacgcgaccg
     2281 tttcgcggtg cggctggcgg cgacggcc
//
LOCUS       DAORPG010000312         2306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5515_length_2306_cov_3.521990, whole genome shotgun sequence.
ACCESSION   DAORPG010000312 DAORPG010000000
VERSION     DAORPG010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5515_length_2306_cov_3.521990"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PLX85_07155"
     CDS             <1..289
                     /locus_tag="PLX85_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRC62984.1"
                     /translation="RRVDAAEALALGLVLDVVPRDDLEARAVALARTYAEGAPLGNAA
                     AKRLVYAVEDPDLTRVQEMSIAYIGQLRNTEDGAEGVNSFLERREPRFRGR"
     gene            378..>2306
                     /gene="dnaE"
                     /locus_tag="PLX85_07160"
     CDS             378..>2306
                     /gene="dnaE"
                     /locus_tag="PLX85_07160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRC62985.1"
                     /translation="MFTHLHTHSEYSLLDGLSRIPDLVARAKEMGQEALAITDHGNLY
                     GAIQFYEEARKHDLKPILGIEAYVAQGSRFDRGAADRSQFHLTLLAQNEAGYRNLLKL
                     STASHLEGFYYKPRMDHELLERHSEGLIALSACPSGEVMSALLEGREQDALNVAGWYR
                     DVFKGRYYIELQEHGLAQFSALNRPLVDLARKLDLPLAVTNDSHYTWQHQHKHHDVLL
                     CIGTNATVHDQNRMRMETDSLYLKSEAEMRALFPELPEAFDNTARIAEQVDIQIPFGR
                     ALLPDPGIPAGLAPIEHLRNLCEEGLRRRYVTPKPEQEERLRYELHVIEETGFVEYIL
                     IVRDIALFARGKGIPMGVRGSAAASIVLYCLGVTDIEPTQYRLVFERFLNLERREMPD
                     VDFDFADDRREEVIRYASERYGRDRVAQIVTFGTLGAKAAIRDSGRALGMSYGDVDRV
                     ARLIPNALHVTLEDAINEVAELRELSASDAQVQELLDTARGLEGVARHASTHAAGIVI
                     SREPLTEVVPLQRATSNKDEEETLPTTQYSMNEVAKIGLVKMDFLGLANLTILGRAVQ
                     LIREQHGIDLDLNTLPDGDESAAAILAEAETFGIFQMESAGMRRYVAELQPRDIRELA
                     AMVALYRPGPMEHIPRYID"
BASE COUNT          389 a          843 c          735 g          339 t
ORIGIN      
        1 ccgccgcgtc gacgccgccg aggcgctcgc gctcggcctg gtgctcgacg tcgtgccccg
       61 cgacgacctc gaggcgcgag ccgtcgccct cgcccgcacg tacgctgaag gcgcgccgct
      121 cggcaacgct gccgcgaagc gcctcgtgta cgccgtggag gatcctgacc tcacgagggt
      181 gcaggagatg agcatcgcgt acatcggcca gctccgaaac acggaggacg gcgccgaggg
      241 cgtgaactcc ttcctcgaac gacgcgagcc tcggtttcgt gggcggtagg gcggcccctt
      301 cgggtggtca ggggtggccg acgggggtat agtcccttcg tacacccgtt cgcgtaattg
      361 cctacacctc tgatcctatg ttcacccacc tccacaccca ctctgagtat tcgctcctcg
      421 acgggctcag ccgcatcccg gacctcgtcg cgcgcgcgaa ggagatgggc caggaggcgc
      481 tggcgattac cgaccacggg aacctgtacg gcgcgatcca gttctacgag gaggcgcgca
      541 agcacgacct caagccgatt ctcggcatcg aggcgtacgt cgcgcagggc agccgcttcg
      601 accgcggcgc cgccgaccgc tcgcagttcc acctcacgct gctcgcccag aacgaggccg
      661 gctaccgcaa cctgctgaag ctgtcgaccg cctcccacct cgaggggttc tactacaagc
      721 cgcgcatgga ccacgagctg ctcgagcggc acagcgaggg cctgatcgcc ctctcggcct
      781 gcccgagcgg cgaggtcatg tccgccctcc tcgagggccg cgagcaggac gcgctcaacg
      841 tcgccggctg gtaccgcgac gtattcaagg ggcgctatta catcgagctg caggaacacg
      901 ggctggcgca gttcagcgcg ctgaaccgcc cgctcgtcga cctcgcgagg aagctcgacc
      961 tcccgctcgc ggtcacgaac gactcgcact acacctggca gcaccagcac aagcaccacg
     1021 acgtgctgct ctgcatcggc acgaacgcga ccgtgcacga ccagaaccgc atgcgcatgg
     1081 agacggactc gctctacctg aagtccgagg ccgagatgcg ggcgctcttc ccggaactgc
     1141 ccgaggcgtt cgacaacacc gcccgcatcg ccgagcaggt cgacatccag atcccgttcg
     1201 ggcgcgcgct gctgcccgac cccggcatcc ccgccggcct cgcgccgatc gagcacctgc
     1261 gcaacctctg cgaggagggc ctgcgccgcc gctatgtaac gccgaagccg gagcaggagg
     1321 agcgccttcg ctacgagctg cacgtcatcg aggaaaccgg cttcgtcgag tacatcctca
     1381 tcgtgcgcga catcgcgttg ttcgcgcgcg gcaagggcat cccgatgggc gtgcgcggct
     1441 cggcggccgc ctcgatcgtc ctgtactgcc tcggcgtgac cgacatcgag ccgacccagt
     1501 accgcctcgt cttcgagcgc ttcctcaacc tggagcgccg cgagatgccc gatgtcgact
     1561 tcgacttcgc tgacgaccgc cgcgaggagg tcatccgcta cgcctcggag cgctacgggc
     1621 gcgaccgcgt cgcgcagatc gtcacgttcg ggacgctcgg cgcgaaggcg gcgatccgcg
     1681 acagcgggcg cgcgctgggc atgagctacg gcgacgtcga ccgtgtcgca cgcctgatcc
     1741 cgaacgcgct ccacgtgacc ctcgaggacg cgatcaacga ggtcgcggag ctgcgggagc
     1801 tgtcggcgtc ggacgcgcag gtccaggaac tcctcgatac cgcgcgtggc ctcgagggcg
     1861 tcgcgcgcca cgcgagcacc catgccgccg gcatcgtcat ttcacgcgag ccgctgaccg
     1921 aggtcgtccc gttgcagcgc gcaacctcga acaaggacga agaggagacg ctgccgacca
     1981 cgcagtactc gatgaacgag gtcgccaaga tcggcctcgt gaagatggac ttcctcggtc
     2041 tggcgaacct gaccatcctc ggtcgcgccg tccagctcat ccgcgagcag cacggcatcg
     2101 acctcgatct caatacgttg cctgacggcg acgagtcggc cgccgccatc ctcgccgagg
     2161 cggaaacgtt cggcatcttc cagatggagt cggcgggcat gcgccgctac gtcgccgagc
     2221 tccagccgcg cgacatccgc gagctcgccg caatggtcgc gctctaccgg cccggcccga
     2281 tggagcacat cccgcgctac atcgat
//
LOCUS       DAORPG010000313         2301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5547_length_2301_cov_4.028940, whole genome shotgun sequence.
ACCESSION   DAORPG010000313 DAORPG010000000
VERSION     DAORPG010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5547_length_2301_cov_4.028940"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /gene="trpB"
                     /locus_tag="PLX85_07165"
     CDS             complement(<1..1035)
                     /gene="trpB"
                     /locus_tag="PLX85_07165"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRC62986.1"
                     /translation="MTNLPDARGHFGEFGGKYVPETLMPALAELEAEYELAKQDPAFH
                     AELETLLREYVGRPSPLTLASRLSAEVGARVYLKREDLNHTGAHKINAVLAQALLARR
                     MGKRRIIAETGAGQHGVATATACALFGLDCVVYMGAEDIKRQALNVFKMKVLGAEVRQ
                     VDSGSRTLKDATNEAIRDWVTNVRDTYYIIGSVVGPHPYPLIVRDFQSVIGREAREQM
                     LEKEGRLPTIAVACVGGGSNAIGLFHAFIEDPVRLIGVEAAGEGLNARHAATLNAGRP
                     GVLHGTKSYLLQDEWGQVLEAHSISAGLDYPGVGPEHAWLKLTERAEYRAVTDTEALA
                     AFQALSRLEGI"
     gene            complement(1032..1772)
                     /locus_tag="PLX85_07170"
     CDS             complement(1032..1772)
                     /locus_tag="PLX85_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRC62987.1"
                     /translation="MTRTKICGNRSPEDVLAAAEAGADFVGMIFANSPRRVDVDEARA
                     MVRALGGPLSEHEFIMPPPAQPHVREEVGEWFRHGASVLESYLEVKRPLTVGVFADQS
                     VDEVNEIAEEAGVDLVQLSGHETWEDCLLVAKQVIQVVHVSPLDSPSDPMSYVEPGFA
                     IALMLDSAHGPYRGGSGQTFDWEVARLVAARIPLVLAGGLNPENVAEAVRRVRPWVVD
                     VASGTETNGRKDYDRVRAFVRAVREADQ"
     gene            complement(1769..>2301)
                     /locus_tag="PLX85_07175"
     CDS             complement(1769..>2301)
                     /locus_tag="PLX85_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HRC62988.1"
                     /translation="FSSGHIADLRAAREEFGDDLDRPALLRKDFLFDPYQVREARANG
                     ADALLLIVMMLEPAALADLIALTHEEGLEALVEVHDESEVEAALAAGARVIGVNNRDL
                     RDFREDLGTFERLARCVPAGTTLVAESSIHTAEDARRMAAAGAHAILVGEALVRSGDV
                     EAKAHELMLVGERVAS"
BASE COUNT          347 a          769 c          832 g          353 t
ORIGIN      
        1 gatgccctcg aggcgcgaga gcgcctggaa ggcggcgagc gcctcggtgt ccgtcacggc
       61 gcggtactcg gcacgctcgg tgagcttgag ccaggcgtgt tcggggccga cgcccgggta
      121 gtcaaggcca gccgagatgg agtgcgcctc gagcacctgg ccccactcat cctgcagcag
      181 gtacgacttg gtgccgtgaa gcacgccggg acggcccgcg ttcagtgtgg cggcatggcg
      241 cgcgttgagg ccctcgccgg ccgcctcgac gccgatgagg cgcaccgggt cctcgatgaa
      301 ggcgtggaag aggccgatcg cgttcgagcc gccgccaacg caggccacgg cgatcgtggg
      361 caagcggcct tccttctcga gcatctgctc gcgtgcctcg cggccgatga cggactggaa
      421 gtcgcgcacg atgagcgggt acgggtgggg cccgacgacc gagccaatga tgtagtaggt
      481 gtcgcggacg ttcgtgaccc agtcgcgaat cgcctcgttg gtggcgtcct tgagggtgcg
      541 ggagcccgag tcgacctggc gcacttcggc gcccagcacc ttcatcttga acacgttcag
      601 ggcctgacgc ttgatgtcct cggcccccat gtagacgaca cagtcgaggc caaagagcgc
      661 gcaggcggtg gcagtggcga cgccatgctg gcccgcgccg gtctccgcaa tgatgcggcg
      721 tttgcccatg cgcctcgcga ggagggcctg ggcgaggacc gcgttgatct tgtgcgcgcc
      781 cgtgtggttc aggtcctcgc gcttgaggta tacccgcgcg cccacttccg cggagagccg
      841 cgaggcgagc gtgagtggtg agggacggcc gacgtattcg cgcagcaacg tctcgagctc
      901 ggcgtggaag gccgggtcct gcttcgccag ctcgtactcc gcctcgagct ccgcgagcgc
      961 gggcatcagt gtctcgggga catacttgcc gccgaactcg ccgaagtggc cgcgtgcgtc
     1021 ggggaggttg gtcactggtc cgcctctcgg acggcgcgca cgaaggcgcg cacgcggtcg
     1081 tagtccttgc gtccgttcgt ctcagtgccg ctggcgacgt cgacgaccca tgggcgcacg
     1141 cgacgcacgg cctcggccac gttctcgggg ttgaggccgc cggcgagcac cagcgggatg
     1201 cgagccgcga cgaggcgcgc gacctcccag tcgaaggtct ggccactgcc gcctcggtac
     1261 gggccgtgcg ccgagtcgag catcaacgcg atggcgaagc ccggctcgac ataactcatc
     1321 gggtcggaag gcgagtcgag tggcgagacg tggacgacct ggatgacctg cttggcaacc
     1381 agcaggcagt cctcccatgt ttcatgaccg gagagctgca ccagatcgac gcccgcctcc
     1441 tcggcaatct cattgacctc gtcgacggac tggtcggcga agacgccgac cgtgagcggg
     1501 cgcttcactt cgaggtagga ctcgaggacg gaggcgccgt ggcggaacca ctcgccgacc
     1561 tcctcgcgga cgtgcggctg cgcgggcggc ggcatgatga actcatgctc ggagagcggc
     1621 ccgccgaggg cgcgcaccat ggctcgcgcc tcgtcgacgt ccacgcggcg cggggagttc
     1681 gcgaagatca ttccgacaaa atcggcgccc gcctcggcgg cggcgaggac gtcctcgggg
     1741 ctgcggttgc cgcagatctt ggtccgggtc acgacgcgac ccgctcgccg acgagcatga
     1801 gctcgtgtgc tttcgcctcg acgtcgccgg acctcacgag ggcttcgccc accagtatgg
     1861 cgtgcgcgcc ggcggccgcc atgcggcgag cgtcctcggc ggtgtggatg gacgactcgg
     1921 cgacgagcgt cgtgcccgcc ggcacacagc gcgccaggcg ttcgaaggtc ccgaggtcct
     1981 cgcggaagtc gcgcaggtcc cggttgttga cgccgatcac gcgggcgccg gcggccaggg
     2041 ccgcctcgac ctccgactcg tcgtgcacct cgaccagagc ctccagcccc tcttcatggg
     2101 tgagcgcgat caggtcggca agcgcggcgg gctccagcat catgacgatg agcagcagcg
     2161 catccgctcc gtttgcgcgc gcctcgcgca cctgatacgg gtcgaacagg aagtccttgc
     2221 ggagcaaggc gggtcggtcg aggtcatccc cgaactcctc gcgggcggca cgcagatcgg
     2281 cgatgtggcc ggaggagaac g
//
LOCUS       DAORPG010000314         2297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5563_length_2297_cov_3.096789, whole genome shotgun sequence.
ACCESSION   DAORPG010000314 DAORPG010000000
VERSION     DAORPG010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5563_length_2297_cov_3.096789"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..340
                     /locus_tag="PLX85_07180"
     CDS             185..340
                     /locus_tag="PLX85_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62989.1"
                     /translation="MVFALILGVLVGGAAALLLQQWVTEALSEPSRTIRPHGAFIERG
                     RSHRRAA"
     gene            427..732
                     /locus_tag="PLX85_07185"
     CDS             427..732
                     /locus_tag="PLX85_07185"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HRC62990.1"
                     /translation="MAVYVMGTVEVIDPERFAPYMEKTPEIIARHGGEVIDIVEAYEV
                     LEGAWPAGAVTAIVRFPDDATARAFWADPDNVAMKDLRHETSRSNVALFRSVPPPTS"
     gene            804..1916
                     /locus_tag="PLX85_07190"
     CDS             804..1916
                     /locus_tag="PLX85_07190"
                     /inference="COORDINATES: protein motif:HMM:NF020318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1786 family protein"
                     /protein_id="HRC62991.1"
                     /translation="MRILAVDIGTGTQDILLFDSSGPVENSPKLVMPSPTSVAARRIR
                     AATAARRAVVLRGVIAGGGPSSWALGDHLKAGLPAFATPDAARTIDDDLDRVQADGVR
                     LVSDEEATRIEGEQVVLRDLDLDAIRAALQAFDVDADFDGIAVGVFDHGAAPPDVSDR
                     VFRFEHIADVLGHDADAHAFACLPGSLPTSLTRARAVLDAVDLNIPAVFMDNGPAAAL
                     GALHDPEVAREQRRAVLNVGNMHVLCFVLDGTRVEGVFEHHSGEVTPEQLAGLVADLL
                     AGRLTNEAVYSSMGHGALYARGFEGGIAPVTMLAVTGPQRERMLPALRAAGLPHVHAA
                     APHGDMMISGCFGLLDGFAYRVPAAREAVAGLHSST"
     gene            complement(1828..2286)
                     /locus_tag="PLX85_07195"
     CDS             complement(1828..2286)
                     /locus_tag="PLX85_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRC62992.1"
                     /translation="MSSDPLPRLRELCLALPEVTERLSHGEPAWFVGGRRSFASYANH
                     HHDDRLAVWCAADEAAQSLLIASDPERFFRPPYVGARGWVGVYLDVPVDWDELAALIE
                     DAYLAVAPKRLAALLEPREARSTSRSAALPPPPAPPAPGTRTRRGGRSSR"
BASE COUNT          360 a          748 c          833 g          356 t
ORIGIN      
        1 gcggtcgagc agcagcggct gcggacgcat gaactgcgct cgatgatcag gcagatgtcg
       61 gaggcgctcg atgacggcgc gcgggccgcg tagtgccgaa ggcgcttgac gaagtagtac
      121 gcctgttcta gcctgattcg caagcctccg cacacatgtt cgggggtcga gagtgaggtg
      181 ggacatggtc ttcgcgctca tcctcggagt gctcgtcggc ggcgcagcgg cactcctgtt
      241 gcagcagtgg gtgaccgagg cgctctcgga gccgtcgcgg accatccgtc cgcatggcgc
      301 gttcatcgag cgcggtcgct cgcatcggcg agccgcgtaa tcgcacgcac cgccgcgacc
      361 ggcacccgcg tatgctctta ccgcactcgc ggttcggcgc ggaccgatcg atgagcggag
      421 ggtggcgtgg cggtctacgt gatggggacg gtcgaggtga tcgacccgga gcgcttcgct
      481 ccgtacatgg agaagacgcc tgagatcatc gcccggcatg gcggtgaggt catcgatatc
      541 gtggaggcgt atgaagtcct cgaaggcgcc tggccggctg gcgccgtcac ggcaatcgtg
      601 cgattcccag acgacgcgac tgcgcgggcg ttctgggcgg atccggacaa cgtggcgatg
      661 aaggaccttc gccacgaaac gtcgcggagc aacgtagcgc tgtttcgctc ggttccacca
      721 ccgacgtcgt aggccctcgg cctgacaggc tcgctggagc gacccctcgc ggctggagag
      781 cgctggcgtc ttacactgag cgcatgcgca tcctggcggt ggacatcggg acggggacgc
      841 aggacatcct gctgttcgac tcatcgggcc cggtggagaa ctcgccgaag ctcgtgatgc
      901 cgtcgccgac ctcggtggcg gcgcgccgca tccgagccgc tactgcagcc cggcgggcgg
      961 tcgtattgcg cggggtgatc gccggtggcg gaccttcgtc ctgggcgctg ggcgaccacc
     1021 tgaaggccgg cctacccgcg ttcgcgacac ccgatgccgc tcgcacgatc gacgacgacc
     1081 tcgaccgtgt acaggcggac ggagtgcggc tcgtgtcgga cgaggaggca acgcgcatcg
     1141 agggcgagca ggtcgtgctg cgcgacctcg acctcgacgc catccgggcg gccctgcagg
     1201 cattcgacgt cgatgcggac ttcgacggca tcgcggtggg cgtattcgac cacggggcgg
     1261 cgccgccgga cgtctcggac cgggtgtttc ggttcgagca catcgccgac gtgctcggcc
     1321 acgacgccga cgcgcacgcc ttcgcgtgcc tccccgggtc gctgcccaca tccctgacga
     1381 gggcccgggc ggtcctcgac gcggtggacc tcaacatccc ggccgtcttc atggacaacg
     1441 gccccgcggc agccctcgga gcactgcacg acccggaggt cgcccgcgag cagcgccgcg
     1501 ccgtcttgaa cgtgggcaac atgcatgtgc tctgcttcgt cctcgatggg acgcgtgtcg
     1561 agggcgtatt cgagcatcac agtggcgagg tgacgcccga gcagttggcg gggctggtcg
     1621 cggacctcct cgcgggccgg ctcacgaatg aggccgtgta ctcctcaatg ggtcacggcg
     1681 ctctctacgc acgcggcttt gagggcggca tcgcgccggt gacgatgctg gcagtgacgg
     1741 ggccccagcg agaacggatg ctgcccgcgc tgcgggcggc gggcctgccg cacgtccatg
     1801 cggcggctcc acatggcgac atgatgatca gcggctgctt cggcctcctc gacgggttcg
     1861 cgtaccgggt gccggcggcg cgggaggcgg tggcagggct gcactcctcg acgtagagcg
     1921 cgcctcgcga ggttcaagca gggcagccag acgtttcggg gcgaccgcga gatacgcatc
     1981 ctcgatgagt gcggcgagtt cgtcccagtc aacggggaca tcgagataga caccgaccca
     2041 accgcgggca cctacataag gaggacggaa gaagcgctcg gggtcggagg caatgaggag
     2101 cgactgcgct gcctcgtcag ccgcgcacca gacggccagg cggtcatcgt ggtggtgatt
     2161 ggcgtagctg gcgaagctgc ggcgaccacc gacgaaccag gcaggctccc cgtgcgagag
     2221 acgctcggtc acctccggta gcgcgaggca cagctcacgc agccgcggca acgggtcgga
     2281 gctcatctcg cgatggc
//
LOCUS       DAORPG010000315         2293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5571_length_2293_cov_3.430742, whole genome shotgun sequence.
ACCESSION   DAORPG010000315 DAORPG010000000
VERSION     DAORPG010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5571_length_2293_cov_3.430742"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="PLX85_07200"
     CDS             complement(<1..1350)
                     /locus_tag="PLX85_07200"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRC62993.1"
                     /translation="MGTDLVVRFAGEGGQGQVTAAEGLAQAAARVGYHVQTFATYPSQ
                     IEGGPVWAQTRVSTNKILTQGDQLDVLVALNRQAYETHIGDLRDGGVLIYNSRDIVEE
                     PPGNVIGLDAEALARDTGNPRAANIIMIGAVAELASMPSEYFEEWIKERFTRGRPNDA
                     EIIGGNVKALEVGIQAARASGYSVESLDEPPAIERQRILVNGNNFLALGALAAGLETF
                     YGYPISPATTILVYMESNLNGADNFVGQASSEIESIAAIVGAGYAGRKVMTSTAGPGL
                     SLMGEGLGLAWMAEIPCVVVDVQRGGPATGLPTKTEQSDLFAALNPGHGDMRVPVIAP
                     GSVAECFQAGVDALNWAERYQGPVILLTEMSIAERNEDVDRPDLSQVKVERRLVSDGS
                     MGNRRYEGAGLTPFPVPGNPGAYVANASEHDEQGDTTHQPYVHINMTERRFSKLKLLE
                     "
     gene            1612..1848
                     /locus_tag="PLX85_07205"
     CDS             1612..1848
                     /locus_tag="PLX85_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRC62994.1"
                     /translation="MVKAYVLIVTNPGSTRTVLDHLRGIPNVQSVHEVMGPYDIVVEL
                     VTDTLTDIPPILSDRIRTVDGIQSTTSLVAFPNE"
     gene            1859..>2293
                     /locus_tag="PLX85_07210"
     CDS             1859..>2293
                     /locus_tag="PLX85_07210"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRC62995.1"
                     /translation="MPVTLEAGDSAPDFDLPDQDGVRHRLSDYRGQTVVLYFYPKDDT
                     PGCTKEACSFRDNELAIRAAGAQILGVSADSAASHQRFRAKHDLPFPLLVDANAEVST
                     SYGAWGEKVLYGRIPGYRLSALGEQMAVRAAEALVDRDVSVVI"
BASE COUNT          362 a          874 c          674 g          383 t
ORIGIN      
        1 ctcgagcagc ttcagcttgc tgaagcgacg ctcggtcatg ttgatgtgta cgtaaggctg
       61 gtgcgtcgtg tcgccctgtt cgtcgtgctc actcgcgttc gcgacataag caccagggtt
      121 gcccggcacc gggaacggcg tcagcccggc gccttcgtag cgccggttac ccatcgaccc
      181 gtccgacacg aggcgccgct cgaccttgac ctggctgagg tctggccggt cgacgtcctc
      241 attgcgctcg gcgatcgaca tctccgttag caggatgacc gggccctggt agcgctccgc
      301 ccagttgagg gcatcgacgc cggcctggaa gcactccgcc acggatccgg gggcaatcac
      361 cggcacccgc atatcgccgt ggccggggtt caacgccgcg aagaggtcgg actgctccgt
      421 cttcgtcggc aggcccgtcg ccggaccgcc gcgctgcaca tccaccacca cgcacgggat
      481 ctcggccatc cacgccaggc ccaggccctc gcccatcagg ctgagtcccg gaccggcggt
      541 cgaggtcatc accttccggc ccgcataccc cgcgcccacg atggccgcga tcgactcgat
      601 ttcagagctg gcctgcccga cgaagttgtc ggccccgttc aggttgctct ccatgtacac
      661 gaggatcgtt gtcgccggcg agatcggata cccgtagaac gtctcgaggc ccgccgccag
      721 cgcacccagc gcgaggaagt tgttcccgtt cacgaggatc cgctgccgct cgatcgcggg
      781 cggctcgtcc agcgactcga cggagtagcc gctcgcacgg gcggcctgga tccccacctc
      841 gagcgccttc acgttaccgc cgatgatctc ggcgtcgttc ggccgcccgc gggtgaagcg
      901 ctccttgatc cactcctcga agtattccga cggcatcgag gccagctcgg ccaccgcgcc
      961 gatcatgatg atgttggccg cgcgcgggtt gccggtgtcg cgggccagcg cctcggcgtc
     1021 caggccgatc acgttccccg gcggctcctc gacgatgtcg cgcgagttgt agatgagcac
     1081 cccgccgtcg cgcaggtcac cgatgtgcgt ctcgtacgcc tgccggttca gcgccacgag
     1141 gacgtccagc tggtcgccct gggtgaggat cttgttggtc gagacccgcg tctgagccca
     1201 tacggggccc ccctcgatct gcgaggggta ggtcgcgaac gtctggacgt ggtaccccac
     1261 gcgcgccgct gcctgcgcaa ggccctccgc cgcggtcacc tgtccctgtc cgccttcacc
     1321 ggcgaatcgg actacgaggt ctgtacccat ggatccctct gaccttacct gaatgcccga
     1381 aacgcgcctc gggaccggct atcgcaacac atggccagca cgaaccgcac ggcccgagga
     1441 ccgctgattc ggctgaccaa catttacacg ccgcggagct tcgaggctcc ccccaaactc
     1501 cgccagtatc aaacgcgtgg cagattacgt tcaacatgcc gaacggcata gtccgcggct
     1561 atgatagcac cgccccgatc gccctcgacc tactccggcc gggaggaacc catggtcaaa
     1621 gcctacgtcc tgatcgtcac caaccccggg tccacccgta ccgtcctcga ccacctgcgc
     1681 ggcatcccga acgtccagtc agtccacgag gtgatgggcc cctacgacat cgtcgtggag
     1741 ctggtcaccg acaccctcac cgacatcccg cccatcctct ccgaccgcat tcgcacggtg
     1801 gacggcatcc agtcgaccac gtccctcgtc gcctttccca acgagtagcg agtaccccgt
     1861 gcccgtcacc ctcgaggcag gcgactccgc ccccgacttc gacctccccg accaggacgg
     1921 cgtccgtcac cgcctcagcg actaccgagg ccagaccgtc gtcctctact tctacccgaa
     1981 ggacgacacc ccgggctgca cgaaggaggc ctgctccttc cgcgacaatg aactggcgat
     2041 ccgtgccgcc ggcgcgcaaa tcctcggcgt ctccgcagac tccgccgcct cccaccagcg
     2101 cttccgcgcg aagcacgacc tgccgttccc gctcctcgtc gacgccaatg ccgaggtctc
     2161 cacgtcctac ggcgcctggg gagagaaggt cctctacggc cgcatccccg gctaccggct
     2221 ctcggccctc ggcgagcaga tggcggtccg tgccgccgag gcgctcgtcg accgcgacgt
     2281 ctcggtcgtc atc
//
LOCUS       DAORPG010000316         2280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5626_length_2280_cov_3.275056, whole genome shotgun sequence.
ACCESSION   DAORPG010000316 DAORPG010000000
VERSION     DAORPG010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5626_length_2280_cov_3.275056"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PLX85_07215"
     CDS             <1..248
                     /locus_tag="PLX85_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62996.1"
                     /translation="RTVVLVVPPGGDGLAAAFVDLEGTRIRVWRRAGVAAVAPWSEQQ
                     LAGLRSLVAGSEDDCLLLVDEHGNAQLVPLLAGAAEL"
     gene            321..836
                     /locus_tag="PLX85_07220"
     CDS             321..836
                     /locus_tag="PLX85_07220"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRC62997.1"
                     /translation="MTWQLDDARTEVSFSVEHLMVATVHGRFTEFEADIVLDPDQPER
                     SHVTATIATASLRTDNAERDRDLLSPGFLNADAYPEIRFVSSDVTRLDEQTFLVRGDL
                     TMHGITRGILGRGRFELLEAAPRRVRFILAAEVDRADFDLTFGIRGVLVGKTVTITVN
                     AEVSEEPSAPA"
     gene            complement(888..1364)
                     /locus_tag="PLX85_07225"
     CDS             complement(888..1364)
                     /locus_tag="PLX85_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC62998.1"
                     /translation="MELGSIVGLPAHPLLVHGAVVLVPLAILGFIAVGWHDGWRRRYL
                     LPVLALAVGGWALAFLATQSGEPMEEHVRSAAAAAGQPRPRFGEHPDLGNTAAILSFV
                     FALAVAGVFALERWVPSFHASRRSMLGAYAVSAVIGLVALIWMVRAGHTGAKLVWG"
     gene            complement(1455..2117)
                     /locus_tag="PLX85_07230"
     CDS             complement(1455..2117)
                     /locus_tag="PLX85_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HRC62999.1"
                     /translation="MSERNLQATFNVAVWGFVALVLAAPWMLAGGTQVPTLWWASRAF
                     GFVSYVALWLSMFTGVLLSTQRLPKQLDRKVLFELHQQWTLAAVIATALHVLAVVTHP
                     EAGVGLAGALVPYASNELTGPVALGVLALWGLGLVAVSSWLRSRISSTTWRLIHTVAF
                     GAFLVALAHSVTAGSDSAALAVQWLYALTGSTLAGAIVLRVASALMGRSRAAVRRPGH
                     AR"
BASE COUNT          377 a          824 c          753 g          326 t
ORIGIN      
        1 gccgtacggt ggtcctcgtc gtccccccgg gcggagacgg gctggccgcc gcgttcgtcg
       61 acctcgaggg aacccgcatc cgcgtctggc ggagggcggg cgttgcggcc gtcgcgccgt
      121 ggagcgagca acagctcgcg gggctgcgct cactcgtcgc cggcagcgag gacgactgcc
      181 tcctgctcgt cgacgaacac ggcaatgcac agctcgtgcc gctgctcgcg ggcgccgctg
      241 agctctgaag gccgggctgc gagggcgcga cggcgctgtg gttcaatgga gacagagccg
      301 gaggagcccg aggagagcgt atgacgtggc agctcgacga cgcgcgtacc gaggtctcct
      361 tctccgtaga gcacctcatg gtcgccaccg tgcacggtcg cttcacggag tttgaggccg
      421 acatcgtgct cgatccggat cagccggagc gttctcacgt gacggccacg attgccacgg
      481 ccagtctccg caccgacaac gccgagcgtg accgcgatct gctgtcacca ggcttcttga
      541 acgctgatgc ctacccggag atccgcttcg tcagctcgga tgtgacccgc ctcgatgagc
      601 agacgtttct cgtgcgcggc gacctcacga tgcacggcat cacccgtggc atcctcggtc
      661 gcggccgctt cgagctgctc gaggccgcgc ctcgccgggt acgcttcatc cttgccgccg
      721 aggttgaccg cgccgacttc gatctcacct ttggcatccg cggcgttctc gtcggcaaga
      781 cggtcaccat caccgtaaac gcggaggtct cggaggagcc ctccgcgcct gcgtagcgaa
      841 cggccgggca agcgcccggc cgtcgtccgt cctcgagtgt ctcggtgcta gccccagacg
      901 agcttggccc cggtgtggcc ggcgcgcacc atccagatca gcgctacgag gccgataacc
      961 gccgagaccg cgtacgcgcc gagcatcgat cgcctcgatg catggaacga gggcacccag
     1021 cgctcgagcg cgaacacccc tgcaacggcg agggcgaaca cgaacgagag gatcgccgcc
     1081 gtgtttccca ggtcggggtg ctcgccgaag cgcggtcgtg gctgtccggc ggcggccgcc
     1141 gcgctgcgca catgttcctc catcggttcc ccgctctggg tcgcgaggaa ggcgagcgcc
     1201 cagcctccga ccgccagcgc cagcaccggc agcaggtagc ggcgtcgcca gccgtcgtgc
     1261 cagccgaccg cgatgaatcc gaggatcgca agcggcacga gcacgacggc gccgtgcacg
     1321 agcaacggat gcgcgggcag tccgacgatc gatccgagtt ccaagttcgt ccctttccac
     1381 tccaccggcg tctcgaagga tgtgggagcc tcaggtcgga gcttccatga tccactgtcg
     1441 ttggctgtaa agcactagcg cgcgtgcccc gggcggcgca ccgcggctct cgaacgcccc
     1501 atcagggcgc tggcgacgcg cagcacgatc gcgcccgcaa gggtcgatcc cgtcagtgcg
     1561 tacagccact gcaccgcgag ggccgcgctg tctgaccctg ccgtgacgga gtgcgcgagc
     1621 gccacgagga acgcgccgaa cgcgaccgtg tggatcagcc gccaggtggt ggacgagatc
     1681 cgcgagcgaa gccagctgct gaccgccacg agccccagtc cccagagggc gagcacgccc
     1741 agagccaccg gccccgtcag ctcgttcgag gcgtacggca ccagcgcccc agcaagtccc
     1801 acgccggcct ccggatgcgt gaccaccgcg agcacgtgca gcgcggtagc gatgacggct
     1861 gcgagtgtcc actgctggtg cagctcgaac aggaccttgc ggtcgagctg cttcgggaga
     1921 cgctgggtgc tcagcagcac acccgtgaac atcgacagcc acagcgccac atacgacacg
     1981 aagccgaacg cgcgcgacgc ccaccacagc gtcggcacct gggtcccgcc cgcgagcatc
     2041 cacggcgcgg cgagcaccag ggcaacaaat ccccacacgg cgacgttgaa cgtcgcctgc
     2101 agattccgct cgctcatcgc aggtacctcg acatccccgg tgtcatttcc catgctcccg
     2161 aaacacgctg aatcagtgcc tggccccccc gggccgcgac cgcgggcatg cctcgccgct
     2221 cgtcggcgag gatcgaggtg gccagcgcgt cagcggtcat cgcatcgctc gcgacgacga
//
LOCUS       DAORPG010000317         2278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5634_length_2278_cov_4.431849, whole genome shotgun sequence.
ACCESSION   DAORPG010000317 DAORPG010000000
VERSION     DAORPG010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5634_length_2278_cov_4.431849"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLX85_07235"
     CDS             complement(<1..323)
                     /locus_tag="PLX85_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005261822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HRC63000.1"
                     /translation="MAFVITQPCIDTQDQACVEVCPVDCIHFEEGADRMLYINPLECI
                     DCGACQPACPVNAIFPQADVPAASQQFIQINELWYSDPAAARGMVGGGGAPAPRPAAE
                     AAPAT"
     gene            complement(431..1426)
                     /locus_tag="PLX85_07240"
     CDS             complement(431..1426)
                     /locus_tag="PLX85_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63001.1"
                     /translation="MHRVVALFLVPFVLLGALAVGCGGSDDAASSSGGSWSSDALSKS
                     QQQLQAQVIGSQIGKGPARLAFGILKPDPKDPDVASLVHDATAHVTLYKLDGDRGTPA
                     GDFDLTPVQLRENTQHQHTDGTQHLHDDPLATVYVVNTTLDQTDWWGASLDIKIADKE
                     YKGVKLRFFVADKTTEPGIGDSVPAIKQLTTKDVPDVAMIDSSTPPRPQLHNLTVADA
                     LQSGKASLIAFATPAFCQTRFCGPVVDSVVAPLQEKYGARMNFLHIEPYKLDEARKGR
                     LIAIPEMAQWGLQSEPWIFVLDANGRVAAKFEGVMSLEEVEPVIQRVLGGQAARN"
     gene            complement(1525..>2278)
                     /locus_tag="PLX85_07245"
     CDS             complement(1525..>2278)
                     /locus_tag="PLX85_07245"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HRC63002.1"
                     /translation="GRVVGVTSLLGLAASMLILLRFVVPGILSGHSPVVIAVIGALTI
                     MASTLFLAHGVNRKTAVALAGTAGALLLTALLASLTIAAADLTGLASEDTAVLQVMSA
                     EHVSATGLLLGGIIIGALGVLDDITIGQASAVFELRAANPLLGPLELYRRAMNVGRDH
                     ISATVNTLVLAYAGASLPLLMLLAVQGESLAIQVNREFIAIEVVRSLVGSIGLVAAVP
                     LTTAIAAAVAGRTSDSAPPEAPDSPELTPALH"
BASE COUNT          368 a          702 c          854 g          354 t
ORIGIN      
        1 tcagtggcag gggcggcctc ggcggcgggc cgaggcgccg gcgcgccacc gccaccgacc
       61 atgccacgag cggctgcggg gtcggagtac cagagttcgt taatctggat gaactgttgc
      121 gaggcggccg ggacgtccgc ctgggggaag atggcgttca ccgggcaggc cggctggcag
      181 gcgccgcagt cgatgcactc gagcggattg atgtacagca tgcggtcggc gccctcttcg
      241 aagtggatgc aatccaccgg gcagacctcg acgcaggcct ggtcttgagt gtcgatgcag
      301 ggctgcgtga tgacgaacgc cacgcgacgc ctcctcaggt agccgggcgc actgcgcccc
      361 ggacgttcaa cggatgcatc ctaccgagcc cccagatgag cgtctacgcg agggccgcgc
      421 ggacgcggtg tcagttcctg gcggcctggc cgccgagtac gcgctggatg acgggctcga
      481 cctcctcgag ggacatgacg ccctcgaatt tggccgcgac ccggccgttg gcgtcgagaa
      541 cgaagatcca cggttcgctc tggaggcccc actgggccat ctcgggaatc gcgatcaggc
      601 gtcccttgcg tgcctcgtcg agcttgtagg gctcgatgtg gaggaagttc atgcgtgcgc
      661 cgtacttctc ctgcagcggc gcgacgaccg agtcgaccac cgggccgcag aagcgcgtct
      721 ggcagaacgc gggcgtcgcg aaggcgatca gcgaggcctt cccgctctgc agggcgtcgg
      781 caacggtgag gttgtgcagc tgcggccgtg ggggcgtcga ggaatcgatc atcgccacgt
      841 ccggcacgtc tttcgtcgtg agctgcttga tggcgggcac ggagtcgccg atgcccggct
      901 cggtcgtctt gtcggcgacg aagaagcgca gcttgacgcc tttgtactcc ttgtccgcga
      961 tcttgatgtc gagcgaagcg ccccaccaat cggtctggtc gagcgtcgtg ttgaccacgt
     1021 agaccgtagc cagtgggtcg tcgtggaggt gctgggtgcc gtcggtgtgt tggtgttggg
     1081 tgttctcgcg cagctggacc ggggtcaggt cgaagtcgcc ggcgggcgtg cccctgtcgc
     1141 catcgagctt gtagagcgtg acgtgggcgg tcgcgtcgtg aaccagcgag gcgacgtcgg
     1201 gatccttcgg atcgggcttg aggatgccaa aggcgagcct cgcgggcccc ttcccgatct
     1261 gtgacccgat gacctgcgcc tgcagctgtt gctggctttt gctcagcgcg tccgaggacc
     1321 acgagccgcc gctcgagctc gccgcgtcgt cggaacctcc gcaccccacc gcgagcgcgc
     1381 cgaggagcac gaacgggacg agaaagagcg cgacgactcg atgcacggcg aagactccga
     1441 tcgctggtcg tgttggcgcc ggggtgagcg aaccacgggc gcggggcgtc tgcgagtggg
     1501 ggcgaggggg gcgcagcgcc gttctcagtg tagcgccgga gtcagttcgg gcgagtccgg
     1561 ggcctcgggc ggggcactgt ccgaggtgcg gccggctaca gcggccgcga tcgccgtcgt
     1621 gaggggcacc gcggcgacga ggccgatact cccgacgagt gaccgcacga cctcgatcgc
     1681 gatgaactcg cggttcacct ggatggcgag ggactcgccc tggacagcca ggagcatcaa
     1741 cagggggagc gaggcgccgg cgtaggcaag gacaagggtg ttcacagtgg cgctgatatg
     1801 gtcgcggccg acgttcatgg cgcggcgata gagctcaagc gggccgagca gcggattggc
     1861 agcgcgcaac tcgaataccg ccgatgcctg gccgatcgtg atgtcgtcga gcacgccaag
     1921 ggcgccgatg atgatgcccc cgaggagcag gccggtcgcc gagacgtgct ccgccgacat
     1981 gacctgcagc accgccgtgt cctcggaagc gagtcctgtg aggtcggcgg cagcgatggt
     2041 gaggctggcc agcaacgccg taagcagcag ggcgccggcg gtcccggcaa gggccacggc
     2101 cgtcttgcga ttaacgccgt gggcgaggaa gagcgtgctg gccatgatcg tgagcgcacc
     2161 gatcaccgcg atcacgacag ggctgtgccc ggagaggatt ccgggcacga caaaacgcag
     2221 gaggatgagc atgctcgccg cgaggccgag gagcgatgtg acgccgacga cgcggccg
//
LOCUS       DAORPG010000318         2275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5650_length_2275_cov_4.600901, whole genome shotgun sequence.
ACCESSION   DAORPG010000318 DAORPG010000000
VERSION     DAORPG010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5650_length_2275_cov_4.600901"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2275)
                     /locus_tag="PLX85_07250"
     CDS             complement(<1..>2275)
                     /locus_tag="PLX85_07250"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRC63003.1"
                     /translation="FVLGLGITGAAGLYAMNRYNSYAEDVVPPEQLIAKLPRGGARIY
                     DRNGTLLYEFVDEFGGLRRPVAIGDIAPVMREATISTEDASFYDNNGLNLKGLVRAGL
                     DNFSPFGGGNMLQGGGGSSITQQLAKNVYIPKEERVERSVDRKLKETVIALELTKKYP
                     KDQILEWYLNSISYGGIYVGVQAASEGYFGKSAKDLSLAEASLLAGIPQSPAAYAPLD
                     HFEAAKERQKEVLDLMVRHGAVTQEQADAAFAQEIVFANKKFDILAPHFVLGPVAKEL
                     EARFGKEALYQGGLEVTTTIDMNLQNLGQQVIEERVASYEQESPTSDGHNGALLAIDP
                     KTGEVLAYVGSRDYFRDDIQGRNDNIAALNSPGSTLKPFTYITTFQQGWSTGTMIFDT
                     PASVKDPSTGQTFSPRNPDSSYHGLVPVSTALASSLNVTAFKAIIFAGVDNVVNTLKK
                     AGLTTLNDERGYGPALTLGGVDIRMDDLTYAYSVLAGGGLMHGQEPVKEHNSGERPLD
                     PIILKKVVTSDGKVLLDNKEPQERRVMGANYAYMATSILSDPKNFCLTYNCAGAGLGL
                     TDRASAVKTGTSEPYENSRKIGETWTFGYTPDLVTGVWAGNSDNSPMSNILSTSISWR
                     AWRDFMTQADQLLQIAPKAFPRPGGLEERDVCWPSGKLATSACPKDRLSKALFAADSI
                     PTDKNKLAQVTDNWWQLVKIDTRTGLLASSSTPAAFVKEESRLVLPAEELAAWPDLRS
                     WVASQGAQLAPTAEESAA"
BASE COUNT          336 a          726 c          783 g          430 t
ORIGIN      
        1 cgccgcgctc tcctcggcgg tcggcgcgag ctgcgcgccc tgtgaggcga cccagctgcg
       61 caggtcaggc caggccgcca gctcctcggc cggcagcacg aggcgagact cttccttcac
      121 gaaggcggcg ggcgtgctcg atgaggcgag caggccggtc cgcgtgtcga tcttcacgag
      181 ttgccaccag ttgtcggtga cctgcgcgag cttgttcttg tccgtcggaa tcgagtcggc
      241 ggcgaaaagc gccttggaga ggcgatcctt cgggcaagcg ctcgtggcga gcttgcccga
      301 aggccagcac acatcgcgct cctcgaggcc gccgggacgc gggaacgcct tcggcgcgat
      361 ctggagaagc tggtccgcct gcgtcatgaa gtcgcgccac gcgcgccacg agatcgaggt
      421 ggaaaggatg ttggacatcg gcgagttgtc ggagttgccc gcccacacgc cggtgacgag
      481 gtcgggcgtg tagccaaagg tccaggtctc gccgatcttg cgcgagttct cgtacggctc
      541 gctggtgccc gtcttgaccg ccgaagcgcg gtcggtcagc ccgagtccag cgcccgcgca
      601 gttgtacgtg aggcagaagt tcttcgggtc cgacaggatc gaggtggcca tataggcgta
      661 gttggccccc atgacgcgcc gctcctgcgg ctccttgttg tcgagcagga ccttgccgtc
      721 cgaggtcacc accttcttca ggatgatcgg gtccaggggg cgctccccgg aattgtgttc
      781 cttcaccggc tcctggccgt gcatcaggcc gccgcccgca aggacgctgt acgcgtacgt
      841 caggtcgtcc atgcggatgt cgacgccgcc gagggtgagc gcggggccgt acccccgttc
      901 gtcgttcagc gtcgtcagtc cggccttctt gagcgtgttc acgacgttgt caacgccggc
      961 gaagatgatt gccttgaacg cagtgacatt gagcgagctc gcgagggccg tcgagaccgg
     1021 cacgagcccg tggtacgagg agtccgggtt ccgcggcgag aacgtctgcc cggtcgacgg
     1081 gtccttcacc gacgcgggcg tgtcgaagat catcgtgccc gtgctccagc cctgctggaa
     1141 cgtcgtgatg tacgtgaacg gcttgagcgt cgagcccggt gagttgagcg ccgcgatgtt
     1201 gtcgttacgc ccctggatgt cgtcgcggaa atagtcgcgg ctgccgacgt aggcgagcac
     1261 ctcgcccgtc ttcgggtcga tcgccagcaa cgcgccgttg tgtccgtccg aggtgggcga
     1321 ttcctgttcg taggacgcga cccgctcctc gatgacctgc tgtccgaggt tctgcaggtt
     1381 catgtcgatg gtcgttgtga cctcgaggcc gccctggtac aacgcttcct tcccgaagcg
     1441 cgcctcgagc tccttggcca ccggaccgag gacgaagtgc ggcgcgagga tgtcgaactt
     1501 cttgttcgcg aagacgatct cctgtgcgaa ggcggcatcc gcctgctcct gggtaaccgc
     1561 gccgtggcgg accatcaggt ccagcacttc cttctgccgc tctttcgcgg cctcgaagtg
     1621 atcgagcggc gcgtaggccg ccggggactg cgggatgccg gccagcagcg aggcttccgc
     1681 gaggctcagg tccttcgcgg acttgccgaa gtagccttcc gaggcggcct gcacgccgac
     1741 gtagatgccg ccgtaggaga tcgagttcag gtaccactcg aggatctgat ccttcgggta
     1801 cttcttcgtc agctcgaggg cgatcaccgt ctctttgagc ttgcggtcga cgctgcgctc
     1861 gacgcgctcc tctttcggga tgtagacgtt cttcgccagc tgctgggtga tcgaggagcc
     1921 gccgccgccc tgcagcatgt ttccgccgcc gaaggggctg aagttgtcga gtcccgcgcg
     1981 gacgaggccc ttcaggttga ggccgttgtt gtcgtagaag ctggcgtcct cggtggagat
     2041 cgtggcctcg cgcatgaccg gagcgatgtc gccgatggcc accgggcgcc gcagcccccc
     2101 gaactcgtcg acgaactcgt agagcaacgt gccattgcgg tcgtagatgc gcgcgccgcc
     2161 acgagggagc ttggcgatca gctgctcggg cgggacgacg tcctcggcgt acgagttgta
     2221 ccggttcatc gcgtacaggc ccgctgcgcc ggtgatgccg aggccgagga cgaag
//
LOCUS       DAORPG010000319         2275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5652_length_2275_cov_3.247297, whole genome shotgun sequence.
ACCESSION   DAORPG010000319 DAORPG010000000
VERSION     DAORPG010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5652_length_2275_cov_3.247297"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLX85_07255"
     CDS             complement(<1..283)
                     /locus_tag="PLX85_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63004.1"
                     /translation="MARLSDAASEPNRVWARGFGVTVRPLVRADLEDPGIQRALRDGI
                     ERNFEGDLPPGQPAAECYAILARQVLVGTLTLRRDVPCAGAVVFDCVAIV"
     gene            complement(271..>2275)
                     /locus_tag="PLX85_07260"
     CDS             complement(271..>2275)
                     /locus_tag="PLX85_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HRC63005.1"
                     /translation="AMAEAVATNIEYSGHLAVEAGTGTGKSLAYLLPALLHALRNDDR
                     VVVSTHTLNLQEQLAQHDLPAAAALVEAHEGVPPGSLRTTVLKGRANYLCLERWAELR
                     STPQPRNQTEARLHARIAVWLPTTETGELGEIAVGATERSAWNALSADSNDCLARRCA
                     YVRDGSCFLLRARARAAAAHVVIVNHALLLTNAATGDQVLPPFRHLVVDEAHRLEGAA
                     TQRYGTTLSLRELEAAIEALGPAGARMRDLAGGQGAPLSPMAGLRGIADALTAAAARV
                     LARIPDLDSTLREFLAEFEDSPGGDQPQLLITAASRAQPAWGDVEVAAAQLDAALLHC
                     NDRLLQARSTLNDVEEGTGPFVDRLRSSLAATSESVSSARWALQDALLRADPASIVWL
                     AGGSGAVRVNIAPLEVADRLASDLYAARSSVAVTSATLTANGSFDYSVRALGLFEPDT
                     LTVPSPFDYRRAVLALVVDDIPTPDEPGYAEAMQRVLADATRAAAGRTLALFTSHGAV
                     RAASEALAAPLAAEGVSVLAHQIDGSPARLLRALVEQPRTLLLGTAAFWEGVDVRGDT
                     LSQIAMARLPFPVPTEPVYAGRAVLYDDPFSEYALPQAVLRFRQGFGRLIRGQDERGV
                     FLLLDRRVLTRPYGDAFLQALPDCELRRVHASEVAPTVAQWLA"
BASE COUNT          349 a          716 c          923 g          287 t
ORIGIN      
        1 gaacgattgc aacgcagtca aagaccacgg ccccagcgca ggggacatcg cggcgcagtg
       61 tcagcgtccc gacgagcacc tgccgcgcca ggatggcgta gcactcagcc gcgggttggc
      121 caggcggcag gtcgccctca aagttccgct cgatgccgtc gcgaagggcg cgctggatgc
      181 cggggtcctc gaggtctgcg cggacgagcg gccgcacggt cacaccgaag ccgcgtgccc
      241 agacgcgatt cggctcgctg gccgcatcgc tcaggcgagc cactgggcga ccgtgggcgc
      301 gacctcggag gcgtggacgc ggcgcagctc gcaatcgggc agcgcctgga gaaaggcgtc
      361 gccgtaaggg cgggtgagga cgcgacggtc gagcaggagg aacacccctc gctcgtcctg
      421 gccacggatc aggcgcccga acccctggcg gaagcgaagc acggcctggg gcagggcgta
      481 ctccgagaag gggtcgtcgt agaggacggc gcgaccggcg tacaccggct cggtggggac
      541 ggggaagggc agccgtgcca tcgcgatctg ggagagcgta tcgcctcgga cgtcgacgcc
      601 ctcccagaag gccgctgtgc cgaggagcag cgtccgtggc tgctcgacga gggcgcggag
      661 gaggcgcgcg ggggagccat cgatctggtg agcgaggacg gatacgcctt cggcggcgag
      721 cggcgcggcg agggcctcgg aagccgcgcg gacggcgccg tgcgaggtaa acagcgccag
      781 agtgcggcca gcggcggcac gcgtcgcatc ggcgaggacg cgctgcatcg cctcggcata
      841 gcccggttcg tcgggggtcg ggatgtcgtc gacaacgagg gcgaggacgg cgcgacggta
      901 gtcgaagggt gagggcactg tgagcgtatc gggctcgaac agcccgagtg cgcgcacgct
      961 gtaatcaaac gaaccgttgg ccgtcagcgt ggcgctggtc acggcgaccg aggagcgggc
     1021 ggcgtagagg tcggaggcga ggcggtcggc gacctcgagg ggcgcgatgt tcacccggac
     1081 ggcgccgctg ccgcctgcga gccagacgat cgaggcgggg tcggcgcgga gcagggcgtc
     1141 ctgtagtgcc cagcgggccg acgagaccga ttcggaggtc gcggccagcg aagagcggag
     1201 gcggtcgacg aaggggcccg tgccctcctc gacgtcgttg agcgtggagc gcgcctgcag
     1261 caaacggtcg ttgcagtgga ggagcgcggc atcgagctgt gccgccgcga cctcgacgtc
     1321 gccccaggcg ggctgggcgc ggcttgccgc cgtgatcaac aactgcggct gatctccgcc
     1381 gggcgagtcc tcgaactccg ccaggaattc gcgcagggtc gagtccaggt cgggaatgcg
     1441 ggcgaggacc cgagcggctg cggcggtgag ggcgtcggcg atgcctcgaa ggcccgccat
     1501 cggggagagt ggggcgccct ggccgccggc gaggtcgcgc atccgggcac ccgcgggacc
     1561 gagcgcctcg attgcggcct ccagctcgcg gagcgaaagc gtcgtcccgt agcgctgggt
     1621 cgccgctccc tcgaggcggt gcgcctcgtc aacgacgagg tggcggaagg gaggcagcac
     1681 ctggtcgccg gtggcggcgt tggtgagcag gagcgcgtgg ttcacgatca cgacgtgcgc
     1741 ggcagcggcg cgggcgcggg cgcgcaggag gaagcagctt ccgtcgcgca cgtaggcgca
     1801 gcgcctcgcg aggcagtcgt tgctgtcggc ggaaagggcg ttccaggcgc tgcgctcggt
     1861 cgcgccgacg gcaatctcgc cgagctcacc cgtctcagtc gtggggagcc agaccgcaat
     1921 gcgcgcgtgg aggcgcgcct cggtctggtt gcgcggctgg ggagtcgagc gcagctcggc
     1981 ccaccgctcg aggcagaggt agttggcacg acccttgagg accgttgtcc gtagcgagcc
     2041 gggcggaacg ccctcgtgcg cctcgaccag cgcggcggcg gcaggcaggt cgtgctgggc
     2101 gagctgctcc tggaggttca gcgtgtgcgt ggaaacgacg acgcggtcgt cgttgcgaag
     2161 ggcgtggagc aacgccggaa gcaggtaggc gagggacttg ccggtgccgg tgccggcctc
     2221 tacggcgagg tgtcccgagt actcgatgtt cgtcgcgacg gcctccgcca tcgct
//
LOCUS       DAORPG010000320         2267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5692_length_2267_cov_3.405063, whole genome shotgun sequence.
ACCESSION   DAORPG010000320 DAORPG010000000
VERSION     DAORPG010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5692_length_2267_cov_3.405063"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PLX85_07265"
     CDS             complement(<1..576)
                     /locus_tag="PLX85_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC63006.1"
                     /translation="MTRILVIDDETDIRQAVALMLARGGYEVVEAANGREGLRRFHAD
                     TPDLVLLDVAMPELDGWQTLDRIRDMSEVPVLMLTARGLESDRVRGLDAGADDYLVKP
                     FGGQELLARVRALLRRVTAAPEVRDSIEHGDLVLDLLQRRVVYDGAEIKLTPLEFRVL
                     QALASHPGNVLSEEQLLERAWNDPSEVGVERV"
     gene            complement(573..2042)
                     /locus_tag="PLX85_07270"
     CDS             complement(573..2042)
                     /locus_tag="PLX85_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRC63007.1"
                     /translation="MQPDDQRSFVFDALSRLPDVDDPTLVRMIEASLAFEALSRLPVM
                     LFALDAEGRYILAEGGGLSGLGVVPSEFVGRSYEDMHAGNDAILDVVHQALHEGRSVE
                     GRMSVGRATFQFSSQPLIRDGVISGVLAAAFDITERERSERLLASVVEESTDAIVVAS
                     AGGEILRFNPAAERMFGYAAAEVLGMPATNLVPPEFRADYAPGTGSAGALGRWVRLRG
                     RCADGEEFPLDLHVSRVAHDHEEPVFVGVMRDASADEASRQARETLLASVSHEFRTPI
                     TAISMFTELARTSPAVPSDVSGYLDVIERNVARLERLVEDLIYLGAGIEEFHAVEESV
                     DIAALVRDAVILARPAADATGIVLDCESAEGPPIPGDPVRLGQLFDNLLGNAVKFTPA
                     GGSIVVRCSVDADAWIVAVTNSSATLRPAEVEGVFEPFRRGAEVITAGVPGQGLGLAI
                     ARAIVERHGGTIELASSAATGTTATVRLPFVRARLGAAS"
BASE COUNT          373 a          721 c          856 g          317 t
ORIGIN      
        1 cacgcgctcg acgccgacct cggaggggtc attccaggca cgctcgagca gttgctcctc
       61 ggagaggacg ttgccgggat ggctcgcgag ggcctggagg acgcggaact cgagcggggt
      121 gagtttgatc tcggcgccgt cgtagacgac gcggcgctgc aacaggtcga ggaccaggtc
      181 accgtgctcg atggagtcgc ggacctcggg tgcggcggtg acgcggcgca ggagggcgcg
      241 cacgcgagcg agcagctcct gccctccaaa gggcttcacg aggtagtcgt cggcgcccgc
      301 atcgaggccg cggacgcggt cggactcgag gccacgggcg gtgagcatga gcacgggcac
      361 ttcgctcata tcgcggatgc ggtcgagggt ctgccagcca tcgagctccg gcatggcgac
      421 gtcgaggagc acgaggtcgg gcgtgtcggc gtggaaacgg cgcagcccct cgcggccgtt
      481 ggcggcctcg accacctcgt agccgccacg agcgagcatc agcgcgacgg cctgccggat
      541 gtcagtttcg tcgtcgatga ccaggatgcg ggtcacgagg cagctccgag ccgggcgcgg
      601 acgaagggga ggcggacggt ggcggtcgtg cccgtggcgg cgctgctggc gagttcaatc
      661 gtgccgccat ggcgctcaac gatggcgcga gcgatcgcga ggccgaggcc ctggccgggc
      721 accccggcgg taatcacctc ggcaccccgg cggaagggct cgaagacacc ctcgacctcc
      781 gccgggcgca gggtcgcgct gctgttcgtg acggcgacga tccaggcatc ggcgtcgacg
      841 gaacagcgga cgacgattga gccgccggcc ggcgtgaact tcaccgcgtt gcccaggagg
      901 ttgtcgaaca gctgcccgag ccgaacgggg tcgccgggaa tcggtgggcc ttcggcggac
      961 tcgcagtcga ggacgatgcc ggtcgcgtcg gccgcggggc gcgcgaggat caccgcgtcg
     1021 cggacgaggg ccgcgatgtc cacgctctcc tcgacggcgt ggaactcctc gataccggcg
     1081 ccgaggtaga tgagatcctc gacgagacgt tccaagcgcg cgacgttgcg ctcgatgacg
     1141 tcgaggtagc cgctgacgtc cgaggggacc gcagggctgg tgcgggcaag ctcggtaaac
     1201 atcgagatcg ccgtgatagg ggtgcggaac tcgtgggaga cggacgcgag cagcgtctcg
     1261 cgggcctgcc gactggcctc gtcggcgctg gcatcgcgca tgacgcccac gaagacaggt
     1321 tcctcgtgat cgtgcgcgac gcggctcacg tgcaggtcga gcgggaactc ctcgccgtcg
     1381 gcacaacgac cgcgcagacg cacccagcgt ccaagggccc ccgcactgcc cgtacccggt
     1441 gcgtaatcgg cgcggaattc gggcgggacg aggttcgtgg cgggcatgcc gagcacctcg
     1501 gcggccgcgt agccgaacat gcgctccgcc gcggggttga agcggaggat ctcgccgccg
     1561 gcggaggcga cgacgatggc gtcggtggac tcctcgacga cgctcgcgag gagccgctca
     1621 ctccgctcgc gctcggtgat gtcaaaggcg gcggcgagga cgccgctgat gacgccgtca
     1681 cggatcaggg gctgagacga gaactgaaac gtcgcccgtc cgacgctcat tctcccctcg
     1741 acgctgcgtc cttcatggag ggcctggtgg accacgtcga ggatggcgtc gttgccggcg
     1801 tgcatgtcct catacgaacg gccgacgaac tccgagggaa cgacgcccag accgctcagg
     1861 ccgccaccct cggcgaggat gtagcggccc tcggcgtcga gggcgaagag catgacgggc
     1921 aggcggctca gcgcctcgaa cgcgagcgac gcctcgatca tgcggacgag cgtggggtcg
     1981 tcgacgtccg gcaggcggct cagggcgtcg aagacgaacg atcgctggtc gtcaggttgc
     2041 atcggcgatg attttatctc gcggagccgg atgcatcgcc ccgcgtaatt gttgacgcgc
     2101 ctccggcgcg gtggtaccac cgacgcgccg gaggtggcac atggacttcg aggttgcgta
     2161 cagcccagag caagagcagt tccgagcgga ggtgcgggcg tggctcgcgg agcacgttcc
     2221 gcaggttgag ggggaccgcg actcggacga gaactatgag aagtacc
//
LOCUS       DAORPG010000321         2266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5695_length_2266_cov_3.156943, whole genome shotgun sequence.
ACCESSION   DAORPG010000321 DAORPG010000000
VERSION     DAORPG010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5695_length_2266_cov_3.156943"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PLX85_07275"
     CDS             <1..780
                     /locus_tag="PLX85_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC63008.1"
                     /translation="GAGSDLGSLQTRAVRDGDEYVINGQKIWTSAAHMADALFMLVRT
                     DPDAPKHRGISFFVLEDIKVPGISVRPLIGANWGHTVNETFFEDVRVPAANLVGEENR
                     GWYVGMTLLDFERSGISGAIQQQEELDELIDAARSDDRSTLARNTSLRARIADRWTET
                     AVSYNFSLRIASMQAAGLVPNYEASMGKMFGSEIGQEIYRTGVRTFGLYSNLWSGDSL
                     APLGGKHIHGYVRSIPATIAGGSSEVQRNIIATRGLGLPRG"
     gene            1306..2031
                     /locus_tag="PLX85_07280"
                     /pseudo
     CDS             1306..2031
                     /locus_tag="PLX85_07280"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
BASE COUNT          330 a          916 c          669 g          351 t
ORIGIN      
        1 ggcgccggct ctgacctcgg ctcgctgcag acgagggccg tgcgcgacgg cgacgagtac
       61 gtcatcaacg gccagaagat ctggacctcc gccgcccaca tggcggacgc cctcttcatg
      121 ctcgtccgca ccgaccccga cgcccccaag caccgcggca tctcgttctt cgtcctcgag
      181 gacatcaagg tccccggcat ctccgtccgc cccctcatcg gtgcgaactg gggccacacc
      241 gtcaacgaga ccttcttcga ggacgtgcgc gtccccgccg ccaacctcgt cggcgaggag
      301 aaccgcggct ggtacgtcgg catgacgctc ctcgacttcg aacgctccgg catctccggc
      361 gccatccagc agcaggaaga gctcgacgag ctgatcgacg ccgcccgcag cgacgaccgc
      421 tcgacgctcg cccgcaacac ctcgctgcgc gcccgcatcg ctgaccgctg gaccgagact
      481 gccgtctcct acaacttctc gctccgcatc gcctccatgc aggcggccgg cctcgtcccc
      541 aactacgagg cctccatggg caagatgttc ggcagcgaga tcggccagga gatctaccgc
      601 accggtgtgc gtaccttcgg cctctactcc aacctctgga gtggcgactc gctcgccccg
      661 ctcggcggca agcacatcca cggctacgtc cgctcgatcc cggccacgat cgccggcggc
      721 tccagcgagg tccagcgcaa catcatcgcc acacgcggcc tgggactccc tcgaggctag
      781 cccaccgccc gcgcacactc acgaggagcg gcgtccgagg gggacgccgc tcctctcgtt
      841 tccggacgcc cgcgcggtac catgcgccag atgccggccc accaccctcc gtccggtact
      901 tctgacgtcc cgcctcgagt gcgtgcggcc gaggtctgcg ccgccatctc cctcgccacg
      961 gatctcggca ccgggcagcc ccttgagcat ggcctccgca ctgccatcct cgccgtccgt
     1021 atcggcgaac tgcacggcct cgcgacgcgc gacctcgtgg acgcgtacta cgttgccctc
     1081 ctccactcgt tcggctgcac gtccgatggc cacgaggccc agcagctcta cggtgatgac
     1141 atcgacccac gtgcggcctt cgcactcctc gacacggcca acgtcgccga gatcgccgcc
     1201 ttcctccagg gtcatgtcgg cgccgaccgc acggcgcccg aacgcgcggc catggtcgaa
     1261 tacgctctcc acaatggaat gaccatcgcc cgccagacct tcgccatgca ctgcgaggtc
     1321 gcccagcgct ttgccggctg gctcggcctg agtcccggga ccgtcgccgc gcttgaacac
     1381 gtctttgagc gctgggacgg caacggcttc cccggcgtca ccgccggcgg ggccctgccg
     1441 ctccccgcga ggatcctgca tgttgcccgc gacatctccg tcttcctgac cgccagcggg
     1501 cccgaggccg ctcgcgccat cctcacggcc cgctccggcg ccgcctacga cccagtcatc
     1561 accgctctcg cgctcgagca cttcgatgca ctgatcgagg ccctcgacga cacgcgcgcg
     1621 tgggcacagg cgctcgaggc tgagcccgca ccccatctct ggttcgcggg tgacgagatc
     1681 gacgccgcgt tcaccgcgat cgcgaccttc gccgacctca agtcgccgtg gtttcgcacc
     1741 cactcgacag gggtggctga cctcgctgag gcagccgcct ggcgtctcgc gctcccgtcc
     1801 ccggacgtca cgaggctgcg ccacgccgcg ctggcgcacg acctcggacg ggtcggcgtc
     1861 acgaacgcca tctgggagaa gccggggccg ctgagcttca gcgagcgcga acgctcgcgc
     1921 cttcacgcac atttcaccga aagggccttc gcgcagtcgg aaaccctggc ttcgatcggc
     1981 gtcctcgccg ggtcacacca tgaacgcctc gacggctccg gctaccatcg gggccgccgc
     2041 tcggtggacc tccctgtcga ggcccgcatc ctcgccgccg ccgaccacta ctactccctc
     2101 tgcgaggcgc gcccctaccg ccccgcctgc gccccggagg acgctgccgc ccgcctccac
     2161 gaggaggtgt cggccggccg tctcgacccg gacgccgtcg ctgctgatct tgcgatatgg
     2221 cgtcttgtcc ttgcccagcg gaaacagcgg cgtgtgcttg tagggc
//
LOCUS       DAORPG010000322         2264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5708_length_2264_cov_2.990041, whole genome shotgun sequence.
ACCESSION   DAORPG010000322 DAORPG010000000
VERSION     DAORPG010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5708_length_2264_cov_2.990041"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..>2264
                     /locus_tag="PLX85_07285"
     CDS             73..>2264
                     /locus_tag="PLX85_07285"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRC63009.1"
                     /translation="MVTAPTPLDADLTESYSIDLRLTTDGRIIDFLHPEVSRPNTPEE
                     RVRQVFARKLHFDYGYPKNLMAIEVPIAVGSETKAADIVVFRSEIAAQVRDQSKINIV
                     VETKAPDVKTGYRQCQSYIFASSAEGGVWINETDAPKYWRRTYDGSQNLEDWPNLPRH
                     GDLWESVGAHTKGALRAPHNLVETFRRCHNALYRQGIDSEDIAMDMVRIILAKYQDEI
                     ADGEDCEFRCTPLELQSTEGRRRVADRVRRLFTEARGNSPDVFDPAEAISAGDREIAT
                     VVSELQYFRFVPDEDSDEVYDIVGAAYEVYIGAHLKGDRGQYFTPRLIVALLTRIVAP
                     GEQDVILDPAMGSGGFLIAAMRQITQAIRRSDRQARSKRAAIRAMQERLYGVDQSPKL
                     VKVARMNMILAADGHAGLVKGDSLHPFAELPTEFARKAGPGKPSVILTNPPFGATTEH
                     RITPSNDPEVLSQFQLGRVWRPDADGRLQPTADYGADGAPPEYLFVERCIRWLAPGGK
                     LGIVVPRGLLDNDKALGLRTLLMRETRVLAVINCHDDSFKPHTDAKAALIFCEKKDTP
                     TDDDPDYPIYMAISQGIGHDGLGKPIFKTDSKGDPILVNEQQVLDEDTEDIYRGWVAL
                     RSGQPSPSEYYYMTSRQALTPALNLNPVRYLPRFAESRRAALELGERQGWATHHLGQI
                     AEVYNGPRFKRPYADSGVTEGPGILRYLTGNAVTQTRLENIKYLDLAKA"
BASE COUNT          447 a          749 c          717 g          351 t
ORIGIN      
        1 cgagaaagtg gcaagagggg agattccggg gcgacactat cgctcgcgcc gcgatcctag
       61 tagagtccgg gcatggtcac tgccccgact cccctagacg ctgacctcac tgagtcatac
      121 tcgatcgacc tccggctcac gaccgatggt cggatcatcg acttcctcca cccggaggtc
      181 agccgcccca acacaccgga ggagcgtgtc cggcaggttt tcgcccggaa gctccacttc
      241 gactacggct accccaagaa cctgatggcg atcgaagtgc cgatcgccgt tggcagcgag
      301 acgaaggccg ccgacatcgt cgtcttcagg agcgagatcg ccgctcaggt acgtgaccag
      361 tcgaagatca acatcgtcgt cgagacgaag gccccggacg tgaagacggg gtacagacag
      421 tgccagtcct acatcttcgc cagctccgcc gagggcggcg tttggattaa tgagacggac
      481 gcgccgaagt actggcgacg gacatatgac ggcagccaga acctcgagga ctggccgaac
      541 ctcccccggc acggcgacct ctgggagagt gtcggcgctc acacgaaggg cgcgctccgc
      601 gcgccgcaca acctcgtcga gacgttccgc cgttgccaca acgcgctcta ccggcagggc
      661 atcgactcgg aagacatcgc gatggacatg gtgaggatca tccttgccaa gtaccaggac
      721 gagatcgcgg acggcgagga ttgcgagttc cgatgtacgc ccctcgaact ccagtccact
      781 gagggccgcc gccgggtagc tgaccgagtg cgcaggttgt tcaccgaggc gcgaggcaat
      841 tcaccggacg tgttcgaccc cgccgaggcg atctcggcag gcgaccgcga gatcgcgacc
      901 gtggtctccg agttgcagta ctttcgattc gtccccgatg aggactcaga tgaggtctac
      961 gacatcgtcg gagcagccta cgaggtctac atcggcgcgc acctcaaggg cgaccggggc
     1021 cagtacttca cgccgcgtct gatcgtcgcg ctgctgaccc gcatcgtggc tccgggagag
     1081 caggacgtaa tcctcgaccc ggcgatgggg tcgggcgggt tcctgattgc cgcgatgcgg
     1141 cagatcacgc aggctatccg ccgcagtgac cgacaggccc gctcgaaacg agccgctatc
     1201 cgagcgatgc aagagcgtct gtacggcgtc gaccaatcgc cgaagctcgt caaggtggcg
     1261 cggatgaaca tgatcctcgc ggcggacggg cacgcggggc tggtcaaggg cgacagcctg
     1321 cacccgttcg ccgaactgcc gacggagttc gcgcgcaagg cgggccccgg caagccgtcc
     1381 gtgatcctga ccaaccctcc atttggtgcg acgactgagc accgcatcac gccgagcaac
     1441 gacccggagg tgctctcgca gtttcagctc ggcagagtgt ggcgcccgga cgccgacggt
     1501 agattgcagc cgacagccga ctacggggcc gatggcgctc cgcctgagta cttgtttgtt
     1561 gaacgctgca tccggtggct tgcgccgggc gggaagctcg gcatcgttgt cccgcgcggg
     1621 ttgctcgaca acgacaaggc gctagggctg cgcacgctgc tgatgcggga gactcgcgtc
     1681 ctcgcggtga tcaactgcca cgacgactcg ttcaagccgc acaccgacgc caaggcggcg
     1741 ctcatcttct gtgagaagaa ggacacgccg acggacgatg acccggacta ccccatctac
     1801 atggccatct cgcaaggcat cgggcacgac ggcctcggga agccaatctt caagacggac
     1861 agtaagggag acccgatcct cgtcaacgag caacaggtgc tcgatgagga cacggaggac
     1921 atttatcgag gatgggtcgc gctccgctcc ggccagccct cgccgtcgga gtactactac
     1981 atgacatcgc ggcaggcact cacgcctgcc ctcaatctca accccgtccg ctacctcccg
     2041 cgcttcgccg agtcccgacg ggctgcgctc gaactcggtg agcgccaagg gtgggcaacg
     2101 caccacctcg ggcagatcgc cgaggtctac aacgggccgc gtttcaagcg cccctacgcc
     2161 gacagcggcg tcaccgaggg gccgggcatc ttgcgttacc tcacgggcaa cgccgtgacg
     2221 cagacgaggc tagagaacat caagtacctc gacctcgcga aggc
//
LOCUS       DAORPG010000323         2258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5723_length_2258_cov_3.067635, whole genome shotgun sequence.
ACCESSION   DAORPG010000323 DAORPG010000000
VERSION     DAORPG010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5723_length_2258_cov_3.067635"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PLX85_07290"
     CDS             <1..361
                     /locus_tag="PLX85_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63010.1"
                     /translation="CRRLIDLDVLPTWPEQPSPAPCLHFIAAWGEGRESMGPEVLHAL
                     EGNRELVIRNVRPAEIARDLLEQQREQVEAAIAAFAHVVDGVALFGDQHERDAVSREV
                     AQILIGPDPMVSRLAAG"
     gene            391..702
                     /locus_tag="PLX85_07295"
     CDS             391..702
                     /locus_tag="PLX85_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRC63011.1"
                     /translation="MPVHDAFPGVVRRTLASGERLTLVEIRIAPGSNVPEHTHPHEQA
                     GHVAAGQVRFRIGSEEFELEAGDSYLIPSGLPHYVEAVDAAVLIEVFSPVREDFVDDA
                     P"
     gene            699..2000
                     /gene="purB"
                     /locus_tag="PLX85_07300"
     CDS             699..2000
                     /gene="purB"
                     /locus_tag="PLX85_07300"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRC63012.1"
                     /translation="MIPRYSRPEMTAIWADDYKFHRWLDVEIAVVQAWADAGVVPAED
                     ARLIASNARIDIGNIDRYIAETHHDVTAFLRSVADSLGPESRWVHLGLTSSDVWDTAT
                     SLQLVAATDVLLAQLARLREVVARRAVEHKDTICVGRTHGVHAEPTTFGLKLAVWVAE
                     IARHEERLRAARATVAVGQMSGPVGTHATVPPTVEEQACAHLGLAVAEVTTQIIQRDR
                     HAHFLAVLAGAGASLEKFATEIRGLQRTEILEAEEPFEAGQTGSSSMPHKRNPELCER
                     VCGLARTLRGYAVTGLENVALWHERDISHSGAERIILPDATGLLAYMLHIFTGVMDGL
                     VVYPQRMRANLERTQGLVFSQKVLLALIETGMTREAAYKIVQRHSMAAWEQQTPLRGL
                     LEGDPEVTERLDAARLDAVFDYGAYLVHVDESFRRIGLLPA"
     gene            1997..>2258
                     /locus_tag="PLX85_07305"
     CDS             1997..>2258
                     /locus_tag="PLX85_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRC63013.1"
                     /translation="MSEVLLTTERPGLLHRGKVRDLYELPGDRMLMVATDRISAFDVV
                     MPQGIPRKGEVLTRLSAYWFDRTAAVVPNHMVAVLEASNASEL"
BASE COUNT          343 a          705 c          882 g          328 t
ORIGIN      
        1 gtgccgacgc ttgatcgacc tcgacgtgct gccgacgtgg ccggagcagc cgtcgccggc
       61 gccatgcctg cacttcatcg cagcctgggg cgaggggcgc gagtcgatgg gcccggaggt
      121 gctgcacgcg ctcgagggga accgggagct ggtgatccga aacgtgcggc cggcggagat
      181 cgcgcgcgat ctcctcgagc agcagcggga gcaggtcgaa gccgcgattg cggcgttcgc
      241 gcatgtcgtc gacggcgtgg cgctgtttgg cgaccagcac gagcgagatg cggtgagtcg
      301 cgaggtagcg cagatcctca tcgggccgga cccgatggtg agcagactgg cggcaggatg
      361 agcgagttcc gaacgaaaac gtacgcagcg gtgcccgtcc acgatgcctt cccgggcgtc
      421 gtgcggcgga cgctggcctc gggtgagcgg ctgacgctcg tcgagatccg gattgcgccc
      481 ggatcgaacg tccccgagca cacgcacccg cacgagcagg ccgggcacgt cgcggcgggc
      541 caggtgcgct ttcgcattgg gagcgaggag ttcgaactcg aggcgggcga ctcgtacctc
      601 atcccgagcg gcctgccaca ttatgtcgag gccgtcgatg cggcggtgct catcgaagtg
      661 ttctcgccgg tgcgcgagga cttcgtggac gacgccccgt gattcctcga tacagccggc
      721 cggagatgac ggcgatctgg gcggacgact acaagttcca tcgctggctg gacgtcgaga
      781 ttgccgtcgt gcaggcgtgg gcggacgccg gcgtcgtgcc cgccgaggac gcgcggctca
      841 tcgcctcgaa cgcccgcatc gacatcggga acatcgaccg ctacatcgcg gagacgcacc
      901 acgacgtgac ggcgttcctg agatcggtgg ccgactcgct gggcccggag tcgcgatggg
      961 tgcacctggg gctgacgtcc tcggacgtgt gggacacggc gacgagcctg cagctcgtgg
     1021 cggcgacgga cgtgctgctc gcgcagctgg cgcggctgcg cgaggtcgtc gcgcggcggg
     1081 cggtcgagca caaggacacg atctgcgtcg gccgaacgca cggcgtgcac gctgagccga
     1141 cgacattcgg gctcaagctc gcggtgtggg tcgccgagat cgcgcgccac gaggagcgcc
     1201 tgcgcgcggc gcgcgcgacg gtggccgtgg ggcagatgtc ggggcccgtg gggacacacg
     1261 cgaccgtacc gccgacagtc gaagagcagg catgcgcaca cctcgggctg gcggtcgcgg
     1321 aggtgacgac gcagatcatc cagcgcgacc ggcacgcgca cttcctcgcg gtgctcgcgg
     1381 gggcgggcgc ctcgctcgag aagttcgcga cggagatccg cgggctgcag cgcacggaga
     1441 tcctcgaggc ggaggagccg ttcgaggcgg ggcagaccgg ttcgtcctcg atgccgcaca
     1501 agcggaaccc ggagctgtgc gagcgggtct gcgggctggc ccggacgctg cgcgggtacg
     1561 cggtgaccgg cctcgagaac gtggcgctct ggcacgagcg ggacatctcg cactcggggg
     1621 cggagcgcat catcctgccc gacgccacgg ggctgctcgc gtacatgctg cacatcttca
     1681 cgggggtcat ggacggcctg gtggtgtacc cgcagcggat gcgcgcgaac ctcgagcgga
     1741 cgcaggggct cgtgttctcg cagaaggtgc tgctggcgct gatcgagacg gggatgacgc
     1801 gcgaggcggc gtacaagatc gtgcagcgtc attcgatggc ggcgtgggag cagcagacgc
     1861 cgctgcgggg cctgctcgag ggggaccccg aggtgactga acgcctcgac gcggcgcggc
     1921 tggacgcggt gttcgactac ggcgcgtacc tcgtgcacgt agacgagtcg ttccggcgta
     1981 tcgggctgct gccggcatga gcgaggtgct gctgacgacg gagcggccgg ggttgctgca
     2041 ccggggcaag gtgcgcgacc tgtacgagct gccgggcgac cgcatgctga tggtggcgac
     2101 ggaccgcatc tccgcgttcg acgtggtgat gccgcagggc atccctcgca agggtgaggt
     2161 gctgacgcgg ctgtcggcgt actggttcga ccgcacggcg gcggtggtgc cgaaccacat
     2221 ggtggcggta ctcgaggcgt cgaacgcctc ggagctgg
//
LOCUS       DAORPG010000324         2235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5835_length_2235_cov_3.280275, whole genome shotgun sequence.
ACCESSION   DAORPG010000324 DAORPG010000000
VERSION     DAORPG010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5835_length_2235_cov_3.280275"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PLX85_07310"
     CDS             <1..458
                     /locus_tag="PLX85_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015135262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRC63014.1"
                     /translation="QARGCSPITTAFENHTLNFVPQKPNTIARSLAIGNPADGYYALK
                     VMEDTQGGSEAATDDEIVEGVKLLAETEGIFAETAGGVTIACLKRLVESGAIRRDEET
                     VAFITGGGLKTIEAVQGKLTAPLRVSANQASFDEALARREAVGVAVGGA"
     gene            458..700
                     /locus_tag="PLX85_07315"
     CDS             458..700
                     /locus_tag="PLX85_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HRC63015.1"
                     /translation="MATKRVRFTFEPQLIREPFIYQLGHEFRIVTNIRMADVDEQVGW
                     VVLELEGDPDEIERGIRWAEAKGVRVDPILGDVVEG"
     gene            737..1012
                     /locus_tag="PLX85_07320"
     CDS             737..1012
                     /locus_tag="PLX85_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRC63016.1"
                     /translation="MPINVKIPTPLRGLTGDRDTVPGQGTTLGELVQHLEAEFPGMRE
                     RLCDDSGSLRRFVNVYVNGEDVRFLSGLDSALKEGDEVSIVPAVAGG"
     gene            1058..2050
                     /locus_tag="PLX85_07325"
     CDS             1058..2050
                     /locus_tag="PLX85_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63017.1"
                     /translation="MPDIQRNSAPISAIVDSPGFAPLKQELRAWVEERLAEALNPGRG
                     RRLKGLPAVLARPLRDLPARERRALKERFRPAAGPRRPLADRLSTLAELRPRLPSDGL
                     FPPGKFDDVLTTLLPRVTYEITGVRCDRSTHEVGADEFYATVIASGPVGAPVLLGPRK
                     LGDFRRGDRATFSPPIVAPALAMANVDGFQSFVALVYCAEVDSGEYLDLMPAIYEELV
                     ADGIVQVIVEGGARGYLATVIVAEVMAALAGLGLALVNPVAGVAVLAAAIAAIVIELA
                     ALWRADEMFTLQVDTLELPVDTHSPPAAQLSPARSLLVSGYGAAYTLTTRWRLA"
BASE COUNT          360 a          808 c          721 g          346 t
ORIGIN      
        1 cgcaggcgcg cggctgctcg cccatcacta ccgccttcga gaaccacacg ttgaacttcg
       61 tgccccagaa gccgaacacg atcgcccgct cgctcgccat cggcaatccc gccgacgggt
      121 actatgcact caaggtgatg gaggacacgc agggcggttc cgaggccgcc accgacgacg
      181 agatcgtcga gggcgtgaag ctcctcgccg agacggaagg catcttcgcc gagaccgccg
      241 gcggcgtaac catcgcctgc ctgaagcggc tcgtcgagag cggcgccatc cgccgcgacg
      301 aggagacggt cgccttcatc accggcggcg gcctgaagac catcgaggcg gtgcagggca
      361 agctcaccgc accccttcgc gtctccgcga accaggcctc gttcgatgaa gcgctggccc
      421 gccgcgaggc cgtcggcgtc gccgtgggag gtgcctgatg gccacgaagc gcgttcgctt
      481 cacgttcgag ccccagctca tccgtgagcc cttcatctac cagctcggcc acgagttccg
      541 catcgtcacg aacatccgca tggcggacgt cgacgagcag gtcggctggg tcgtcctcga
      601 gctcgaaggc gacccggacg agatcgaacg cggcatccgc tgggccgagg ccaaaggcgt
      661 acgagtcgat ccgatcctcg gcgatgtcgt cgagggctaa tccaccagtc agcgaacgga
      721 cagagacgga agaccgatgc ccatcaacgt aaagatcccc accccgctgc gcggcctgac
      781 cggcgaccgc gacaccgtac ccggccaggg caccacactc ggcgagctcg tccagcacct
      841 cgaggcggag ttccccggta tgcgcgagcg cttgtgcgac gactccggct cgctgcgccg
      901 cttcgtcaac gtctacgtga acggcgagga cgtccgcttc ctgagcggcc tcgacagcgc
      961 cctcaaagaa ggcgacgagg tttccatcgt ccccgccgtc gccggcggct aagcgctatt
     1021 cgcctgcccg acctcgaccc ggctgcggag gtgagcggtg cccgacatcc aacgcaacag
     1081 cgcgccgata tcggcgattg ttgacagccc gggcttcgcg cccctgaagc aggagctccg
     1141 cgcctgggtc gaagaacgcc tcgctgaggc gctgaaccct ggacgcggac gtcgcctgaa
     1201 gggcctcccg gccgtcctcg cacggccgct ccgggacctg ccggcgcgtg aacgccgggc
     1261 gctgaaggag cgtttccgcc cggccgcggg cccgcgtcgg ccgcttgccg atcgtctgag
     1321 caccctcgcc gaactccgcc cgcggcttcc ctcggacggc ctcttcccgc cgggcaagtt
     1381 cgacgatgtc ctgaccaccc tgcttccgcg cgtgacttac gagatcaccg gcgtccgctg
     1441 cgatcggtcg acccacgagg tgggcgccga cgagttctac gcgacggtga ttgccagcgg
     1501 gccggtgggc gctccggtac tgctcggtcc ccggaagctt ggcgacttca ggcgcggcga
     1561 ccgcgctaca ttctcgccgc ccatcgtggc ccccgctctg gcgatggcca acgtcgatgg
     1621 cttccagtca ttcgttgccc tcgtctactg cgccgaggta gacagcggcg agtacctcga
     1681 tctcatgccg gccatctacg aggagctcgt ggctgacggc atcgtccagg tcatcgtcga
     1741 aggtggcgcc cgcggctacc tcgcgacggt gatcgtggcc gaggtgatgg ccgcgctcgc
     1801 cggcctcggg ctcgctctgg tgaaccccgt ggccggcgtc gccgtgctgg ctgcagccat
     1861 cgccgcaatc gtgatcgagc tcgccgctct gtggcgggcc gatgagatgt tcacgctgca
     1921 ggtcgatact ctcgagctcc cggtcgacac acacagtccg cccgccgcgc agctttcccc
     1981 ggcgcgctcc ctgcttgtgt ccggttacgg tgcggcatat accctgacga cacgctggcg
     2041 cctcgcctga cggtcagcgc ctcgtcgcga cggcacacgg gcccaatggt agtatcggcg
     2101 ccgctcacac agcgcgaatg actcgcgggt tcgcgagacg cacgaatctc gaggaggaat
     2161 catggactgg ggcgacacgc ccgaacaggg gcaattccgg cagcaggtgc gggagttcat
     2221 ccacagcaac ctgcc
//
LOCUS       DAORPG010000325         2234 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5841_length_2234_cov_3.098669, whole genome shotgun sequence.
ACCESSION   DAORPG010000325 DAORPG010000000
VERSION     DAORPG010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5841_length_2234_cov_3.098669"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="PLX85_07330"
     CDS             <1..940
                     /locus_tag="PLX85_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63018.1"
                     /translation="GTQAERLAAIEAGYIGLFVLPLTAGALLALRRLGPGTPARTWVA
                     FVIWMTAVVSGMAALRALGMRMPYVPHFLSMAGLGPNDLLVARSPLAGLWFFTALTLA
                     CAVSAVVAGFLVIRAVSARLPSANVTMVLVAFGIQAVLVVIVSTHFRYWTVAGLPAPS
                     LDRYLLPLLPLALALVLWSVRDVDVHLPLTWYVVGVLAVFAVVGTRDNLAFHQAQWAL
                     AQEAVDSGIPLTQLDGGGSWTGYYLGERSYAERGIHNNGDARWWLTLYGSIIDPEYAI
                     ATKELPGYTTVSTYPYDLWLDTRPTALYLLHRNGTP"
     gene            1062..1481
                     /locus_tag="PLX85_07335"
     CDS             1062..1481
                     /locus_tag="PLX85_07335"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRC63019.1"
                     /translation="MRFAGVGFIATATDFLVFNAALAGNTHPGPIHVILANTSAFAVA
                     TLVGYALNARLTWGMSHRRQLLLRYAGVALLGALVYDAVLVGLLEATQADGYLALNAI
                     KIAAVAVSATWNFIGFNFFVFRDPEARQPAAASEAGS"
     gene            1478..2203
                     /locus_tag="PLX85_07340"
     CDS             1478..2203
                     /locus_tag="PLX85_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC63020.1"
                     /translation="MSSSLTVIIPAWNESQRIGATLDHLIDRCQGLDIAEVIVVNDGS
                     SDDTAELVRARAQRATGPCIRLIEHERNQGKGAALRTGLAAARTELIGYLDADLSVGP
                     EPFKDATREIEAGADVIVGVRISHTGRADGAGQPWLRGQLSRLFKRFQRRLIGLPLQD
                     TQCPFKLFTSDAVARTLPRCTSDGWGFDVEVLLVADRSGLRVREIPVPWHFVGGSTVR
                     ANPLTAWRTLREVLAIRRRHGSV"
BASE COUNT          297 a          732 c          838 g          367 t
ORIGIN      
        1 cgggacgcag gcggaacggc tcgcagcgat cgaggcgggg tacatcgggc tgtttgtgct
       61 gccgttgacg gcaggtgcgc tgctcgccct gcgccggctg ggaccaggga cgcccgcgcg
      121 cacctgggtc gcgttcgtca tctggatgac cgctgtggtg tcgggcatgg cggcgctgcg
      181 ggcgctcggg atgcggatgc catacgtgcc gcacttcctg agcatggctg gcctcggacc
      241 gaacgacctg ctcgtggcaa ggtcaccgct ggcggggctg tggttcttca cggccctgac
      301 gctggcgtgc gccgtctcgg ccgtggtggc agggttcctc gtgattcggg cggtgagcgc
      361 gcggctcccg agcgcgaacg tgacgatggt gctggtcgcg ttcggcatcc aggcggtgct
      421 cgtcgtgatc gtgtcgacgc acttccgcta ctggacggtg gcggggctgc ccgccccctc
      481 gctggaccgc tacctgctgc cgctgttgcc gctcgcgctc gcgctggtgc tgtggtcggt
      541 gcgggacgtc gacgtgcacc tgccgctcac ctggtacgtg gtcggagtac tggcggtgtt
      601 tgccgtggtc gggacgcgcg acaacctcgc gttccaccag gcgcagtggg cgctggcgca
      661 ggaggcggtg gactcgggga tcccgctgac gcagctcgat ggcggggggt cgtggacggg
      721 ctattacctc ggcgagcgtt cgtacgcgga gcgaggcatc cacaacaacg gcgacgcgcg
      781 gtggtggctc acgctgtacg gctcgatcat cgacccggag tacgccatcg cgaccaagga
      841 gctgcccggg tacacgaccg tttcgacgta tccgtacgac ctctggctcg atacacgacc
      901 gaccgcgctc tacctgctgc atcgaaacgg gacgccctga cctccgcggc gcggtgaacg
      961 ggttgcgggc gactcgcagg ggcggatact gtcgccggag cagaaagggc ggtgccgtgc
     1021 gacgcccccc gcgcctcacg gcccttcttc acgcccgcct cgtgcggttt gccggcgtgg
     1081 gcttcatcgc gacggcgacc gacttcctcg tgttcaacgc ggcgctggca ggcaacaccc
     1141 acccggggcc gatccacgtc atcctcgcca acacctcggc gttcgcggtc gcgacactcg
     1201 tggggtacgc gctcaacgcg cggctgacct gggggatgtc gcaccgccga cagctgctcc
     1261 tgcggtacgc aggcgtcgcg ttgctcggcg cgctggtcta cgacgccgtg ctcgtcgggt
     1321 tgctggaggc gactcaggcc gacggctacc tggcgctgaa cgcgatcaag atcgcggcgg
     1381 tcgcggtttc ggcgacctgg aacttcatcg ggttcaactt cttcgtgttc cgtgaccccg
     1441 aggcgcggca gccggcggcg gcaagcgagg cgggctcgtg agctcgagcc tgacggtgat
     1501 catcccggcg tggaacgaat cgcagcgcat cggcgcgacg ctcgaccacc tgatcgaccg
     1561 ctgccagggc ctcgacatcg ccgaggtgat cgtggtgaac gacgggagct cggatgacac
     1621 ggctgaactc gttcgtgcgc gagcgcagcg ggcgacaggc ccgtgcatcc ggctgatcga
     1681 gcacgagcgg aaccagggca agggggccgc gctgcggacc ggcctcgcgg ccgcgcgaac
     1741 ggagctgatc gggtacctcg acgcggacct gtccgtgggg cccgagccgt tcaaggacgc
     1801 cacccgggaa atcgaggccg gcgcggacgt catcgtggga gtgcgcatct cacacacggg
     1861 ccgcgctgac ggcgcgggcc agccgtggct gcgcgggcag ctgagccggc tgttcaagcg
     1921 cttccagcgg cggctgatcg ggttgccgct gcaggacacg caatgcccgt tcaagctctt
     1981 cacgagcgat gccgttgccc ggacgctgcc gcgttgcacc tcggacggct ggggctttga
     2041 cgtcgaggtg ttgctggtgg cggaccggag cgggctgcgg gtgcgggaga tcccggtgcc
     2101 gtggcacttc gtgggggggt ccacggtgcg ggcgaacccg ctgacggcgt ggcgcacgtt
     2161 gcgcgaggtg ctggcgatcc gccgccggca tgggtcggtc tgagcatgga cgcgacggag
     2221 caccagcggc tccc
//
LOCUS       DAORPG010000326         2233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5848_length_2233_cov_3.323232, whole genome shotgun sequence.
ACCESSION   DAORPG010000326 DAORPG010000000
VERSION     DAORPG010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5848_length_2233_cov_3.323232"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PLX85_07345"
     CDS             complement(<1..243)
                     /locus_tag="PLX85_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HRC63021.1"
                     /translation="MSEIHFGTDGWRGVIADDYTFANVRLAAQGTANQLRAAGQAERG
                     IVVGYDTRFLSSTFASLVAEVMAGNGIRVWLASEHCP"
     gene            complement(254..1348)
                     /locus_tag="PLX85_07350"
     CDS             complement(254..1348)
                     /locus_tag="PLX85_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HRC63022.1"
                     /translation="MRAIVLVGGEGTRLRPLTLRTPKQLVPVLNRPLLDHLLLQLKRH
                     GIASITLAMTRRSEAVREAFGDGERLGLDLEYAYEETPLGSGGAIASIAAGWRERFLV
                     CNGDIVTDLDVTAFVEAHQARGAELSIALHEVEDPSAFGVVALDGAERITRFVEKPKR
                     EDAPSRWINAGFWLFEPSLVAEMDGTSFHRVEDGLFPRLAGGGRGIFGFRHHGYWMDV
                     GNPEAYRRVNLDLLGGACAAGLPDPWATGGIVTEGAEVASGARVSPPALLGTGTRLDA
                     GATIEGPAVAGARCTLHEGAKVSSSVLWDDVTVGAGARVLDTVVASGARIEAGAHVEG
                     AVIGHYATVAAGVYVHRGTRVEPDTVYTGE"
     gene            1523..1879
                     /locus_tag="PLX85_07355"
     CDS             1523..1879
                     /locus_tag="PLX85_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63023.1"
                     /translation="MFEQAPRPVIWAAALTVFNAVVGFAVAAVWPDIDDRGTLIVVGG
                     VLTLLMLGATLWLWRRAKWGGIAFIAINAVNALSALPALFAGDAALAVIGVISALLSV
                     AAIVFVLRPEAKPYWS"
BASE COUNT          332 a          847 c          690 g          364 t
ORIGIN      
        1 cgggcagtgc tccgaggcga gccacaccct gatcccgttc cccgccatca cctccgcgac
       61 cagcgaggcg aacgtcgagg agaggaaccg cgtgtcgtac ccgaccacga tcccgcgctc
      121 cgcttgcccg gcggcccgca actggttcgc tgtcccctgt gctgccagcc gcacgttcgc
      181 gaacgtgtag tcgtcggcga tcacgccgcg ccacccgtcg gtcccgaagt ggatctcgct
      241 catgaccgcc ccattactcg ccggtgtaca ccgtgtccgg ctcgacgcgc gttcctcgat
      301 gcacatacac cccggccgca accgttgcgt agtgcccgat gaccgcccct tcgacgtgcg
      361 cccccgcctc gatccgcgct ccgctcgcca ccaccgtgtc gagcacacgc gctcccgctc
      421 ccaccgtgac atcgtcccac agcaccgagg aagagacctt cgccccctcg tgcaacgtgc
      481 agcgcgctcc tgcgaccgct ggcccctcga tcgtcgcgcc cgcgtcgagg cgcgttcccg
      541 tccccagcag cgcgggcggg ctcactcgcg ctcccgaagc gacctcggcc ccctcggtca
      601 cgatgccgcc tgtcgcccac ggatcgggca gcccagcggc gcacgcgccc cccagcaggt
      661 cgaggttcac ccgccggtac gcctccgggt tccccacgtc catccagtag ccgtggtgcc
      721 ggaatccgaa gatcccgcgg ccgcccccgg ccagccgggg gaacagcccg tcctcgaccc
      781 ggtggaagct cgtcccgtcc atctccgcga ctagggaggg ctcgaatagc cagaaccccg
      841 cattgatcca ccgtgagggc gcatcctcgc gcttcggctt ctccacgaac cgtgtgatcc
      901 gctccgcccc gtccagcgcc acgaccccga acgccgaggg gtcctccacc tcgtgcagcg
      961 caatcgagag ctctgcgccg cgcgcctggt gcgcctcgac gaaggccgtc acgtcgaggt
     1021 cggtcacgat gtccccgttg cagacgagga accgttcccg ccagccggcc gcgatcgagg
     1081 cgatcgcgcc tcccgatccc agtggcgtct cctcgtacgc gtactcgagg tcgaggccca
     1141 gtcgctcccc gtccccgaag gcctcgcgca ccgcctcgct ccgtcgcgtc atcgcgagcg
     1201 tgatcgaggc gatgccgtgt cgcttcagct gtagcagcaa gtggtcgagc agtggtcggt
     1261 tcaacaccgg gaccagctgc ttcggcgtcc gcaacgtcag cggtcgcagc cgggtcccct
     1321 caccacctac taggacgatc gcgcgcatgc caagactcta cccgcccgcc cctcccgagg
     1381 gccatctggg gtggcgggcg agtcacgtgt gctgcgccag catgattgcc tccatggcag
     1441 accgaggctg ccttgaggcg tataaacgaa ccggcgaccg cctgatcgcc gaggcgccgg
     1501 catccgggtg gaggaagaaa ccatgtttga gcaggcaccg agaccagtca tctgggctgc
     1561 cgccctgacg gtgttcaatg cggtcgtggg attcgccgtc gcggccgtat ggcccgacat
     1621 agacgaccgc gggacgctga tcgtcgtcgg gggagtcctc acgttgttaa tgctcggcgc
     1681 tacgctctgg ctgtggcgcc gagcgaagtg gggcggcatc gccttcatcg cgatcaacgc
     1741 cgtcaatgcc ctgtcggccc tgcccgcgtt gtttgccggg gatgcggcct tggcggtgat
     1801 cggcgtcatc tccgcgctgc tcagcgtggc ggccatcgtc ttcgtgctgc ggcccgaagc
     1861 gaaaccgtac tggagctaac tccgccggtg cagcgagtga cccggtagca aggcaggcgc
     1921 tgacctcagc gtgacggtga cggacatccc gttctccgct cccacgtggc gcccggtgct
     1981 gcctatgccg cccgcgctgc ctcggcgccg tgcgcgttgg cctcgaggcg ttcgcgcgcc
     2041 tcgatcaaaa tcctgagctt gccggccagt tcgagtgtcg ccacacgttt ctccacaagg
     2101 cctcatactg aacgaggcca aggcttgggc tgtcaatggg gcggtcgacc ggtcaacgcg
     2161 accaagagcc ctgtccgcct ggattgccca ggacacgcaa cacgtatagg acctgcgcct
     2221 cgactgtcgc gtg
//
LOCUS       DAORPG010000327         2232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5856_length_2232_cov_3.554892, whole genome shotgun sequence.
ACCESSION   DAORPG010000327 DAORPG010000000
VERSION     DAORPG010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5856_length_2232_cov_3.554892"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1478)
                     /locus_tag="PLX85_07360"
     CDS             complement(<1..1478)
                     /locus_tag="PLX85_07360"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRC63024.1"
                     /translation="MFKRVLIANRGEIALRVQRTCRELGIETVAVYSEPDRNALHVRL
                     ADHAVCIGPGPSTESYLVIDKIIEAARATGADAIHPGYGFLSENPRFVDACEASGITF
                     IGPPASAMRALGDKVSARKLMETAGVPVVPGANDISADDFETARREAERIGFPLLVKA
                     AAGGGGRGIRVVERIEDLEGAMRASGAEAASSFGDATIFLERFVTEARHIEVQMMADQ
                     HGNVRAYGERECSVQRRHQKLVEEAPSVAVSPELRKRLCDAAVAAAKSCGYQNAGTVE
                     FLLDKSGEFYFLEVNARLQVEHPVTELVYSVDLVKQQLEVASGEALPPLEGPLEPKGW
                     AIECRINGENPYLNFMPSLGLVERVEVPLGPGTRFDSMLFDGLDVPVFYDSMLGKLIV
                     WAEDRELAIKRMRRALHDLLIAGVDTNVPFHLALLDNEDFQAGRIHTNWLENSFTMPA
                     LPEDDPRGMTALIAAALASGMVASAAPASAGAPASSRWLRAGR"
     gene            1597..2199
                     /locus_tag="PLX85_07365"
     CDS             1597..2199
                     /locus_tag="PLX85_07365"
                     /inference="COORDINATES: protein motif:HMM:NF022114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HRC63025.1"
                     /translation="MTRRTMPAAVLAFLVLTLALVACGGDESSTPTATATVSSTPAPS
                     STVTATGTASATATGTPFATSTPSPTSTATSGAPAGYATSCAAGYPWGQTVTGPFVCI
                     QFPNSGITPTGSVPVSGYAGGSFENNVVVALRDQNNATLLQQSLTYTAPDVGKPGTWQ
                     TTVALPPGQPSGSPGRIIVYFTSPRDGQVVALDSIELRFP"
BASE COUNT          345 a          841 c          705 g          341 t
ORIGIN      
        1 cgcccggcgc gcagccaccg gctgctcgcc ggcgctcccg ccgacgccgg agccgcactc
       61 gcgaccatcc cggaggcgag cgccgccgcg atgagcgcgg tcattcctcg agggtcgtcc
      121 tccggcaggg ccggcatggt gaacgagttc tcgagccagt tcgtgtggat gcgccccgcc
      181 tggaagtcct cgttatcgag gagcgccagg tggaatggca cattcgtgtc tacgcccgcg
      241 atcagcaggt cgtgcagcgc gcgccgcatc cgcttgatcg caagctcgcg gtcttccgcc
      301 cagacgatca gcttgccgag catcgagtcg tagaagaccg gtacatcgag cccgtcgaag
      361 agcatcgagt cgaagcgcgt accaggcccc agcgggacct cgacgcgctc gacgagcccc
      421 aggctcggca tgaagttcag gtacgggttc tcaccgttga tgcggcactc gatcgcccag
      481 ccctttggct ccagcggccc ttcgagcggc ggcagcgcct cgcctgacgc cacttcgagc
      541 tgctgtttca cgaggtccac gctgtagacg agctccgtga ccgggtgctc cacctgcagc
      601 cgcgcgttga cttcgaggaa gtagaactca ccgctcttgt cgagcaggaa ctcgaccgtg
      661 ccagcgttct ggtagccaca gctcttcgct gccgccaccg ccgcgtcgca cagccgcttc
      721 cgtagctccg gcgacaccgc cactgacggc gcctcctcga cgagcttttg gtgacgccgc
      781 tgcacggagc actcacgctc gccgtaggca cggacgttgc cgtgctggtc ggccatcatc
      841 tgcacctcga tgtgccgcgc ctcggttacg aagcgctcga ggaagatcgt cgcgtccccg
      901 aaggaggacg cggcctcggc ccccgaggct cgcatcgcgc cctcgaggtc ctcgatgcgc
      961 tcgaccacac ggatgccgcg cccaccgccg ccggcggcgg ccttcaccag cagcgggaag
     1021 ccgatgcgct cggcctcgcg gcgagccgtc tcgaagtcgt cagcgctgat gtcgttcgcg
     1081 ccgggcacca cggggacgcc tgccgtctcc atcagcttgc gcgccgagac cttgtcaccg
     1141 agggcccgca tggccgaggc cggtggcccg atgaaggtga tcccggacgc ctcgcaggcg
     1201 tcgacaaagc gtgggttctc cgacaggaag ccgtagcccg ggtggatcgc gtccgcgccc
     1261 gtcgcacgcg ccgcctcgat gatcttgtcg atcaccaggt aggactcggt cgaggggcca
     1321 gggccaatgc acactgcgtg gtcggcgaga cgcacatgca gcgcgttgcg atcgggctcg
     1381 gagtagacgg ccacggtctc gatgccgagc tcgcggcagg tacgctgcac acgcagcgcg
     1441 atctcgccgc gattcgcgat gagcacgcgt ttgaacaacg ctggtcgcct ccacatgcca
     1501 cggttgagag agtggccgta gcctcgcacg tccgcgccag tgcggcaacc gtacgccatc
     1561 gtccgccggt tgacccgctc tggcatgctg gtcgctatga cccgtcgaac gatgcccgcc
     1621 gccgtcctcg cgttcctcgt gctcaccctc gcgctggtgg cctgcggcgg cgacgagtcg
     1681 tccacaccca ccgcaacagc gaccgtgagc tcgacgccgg ctcccagctc gaccgtcacc
     1741 gcgacgggaa ccgcctcggc caccgcaacc ggcaccccct tcgcgacctc gacaccgtca
     1801 ccaacgagca cggcgacgtc cggcgcgccc gccggctacg cgacctcctg cgcggcggga
     1861 tacccctggg gccagacggt gaccggcccg ttcgtgtgca tccaattccc caatagcggc
     1921 atcacgccga ccggcagcgt cccggtgagc ggctacgccg ggggctcctt cgaaaacaac
     1981 gtcgtcgtgg cgctccgcga ccagaacaac gcgacgctgc tccagcagtc cctgacgtac
     2041 accgcccccg acgttggcaa gcccggcacg tggcagacca cggtcgcgct gccgcccggc
     2101 cagccatccg gctcaccggg ccgcatcatc gtctacttca ccagcccccg ggacggccag
     2161 gtcgtcgccc tcgacagcat cgagttgcgc ttcccgtagc cgcctctcac ctcattccga
     2221 ggcgcgcgag tc
//
LOCUS       DAORPG010000328         2222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5896_length_2222_cov_2.568066, whole genome shotgun sequence.
ACCESSION   DAORPG010000328 DAORPG010000000
VERSION     DAORPG010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5896_length_2222_cov_2.568066"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PLX85_07370"
     CDS             <1..458
                     /locus_tag="PLX85_07370"
                     /inference="COORDINATES: protein motif:HMM:NF037816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HRC63026.1"
                     /translation="PAAATAAATRQSTRTPTASTGSASSQAPRRDLAADEAAGGHTLE
                     RHVGRTDEQLRQRLRDEPDISAASTYTDRDTAERVVGAALQRNQAKIADWVKQGRTRT
                     NLALDYEAGEVIGRSIPRGKTVAHTVTAATVVLRADGSSWFVLTSYPDD"
     gene            451..753
                     /locus_tag="PLX85_07375"
     CDS             451..753
                     /locus_tag="PLX85_07375"
                     /inference="COORDINATES: protein motif:HMM:NF036906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
                     /protein_id="HRC63027.1"
                     /translation="MTSGGPPGTDSLRKMLRGYFHQDVIAEHGSIEAAARAYRAEAPP
                     AERWRAARQLRVLLAGCETVEDVRDAFAALRSGWRPRDLRSVERVLAILETDGSVS"
     gene            complement(805..1440)
                     /locus_tag="PLX85_07380"
     CDS             complement(805..1440)
                     /locus_tag="PLX85_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC63028.1"
                     /translation="MTDVPIRVVIVDDHELVRIGISQFLSGQANIEVVGTAGDGESAV
                     RLAGELQPAVVLMDLAMPGVDGVEATRRLQETAPSARVIVLTSFSDRSRILDALDAGA
                     VGYLLKDAEPAELIRGVEAAARGESPLSPKVANALLEERRVREAGRQLTPREREVLVL
                     LARGLLNKQIARELGISEKTVKAHLVNVFDRIGVSDRTQAALWAERRGLVR"
     gene            complement(1437..>2222)
                     /locus_tag="PLX85_07385"
     CDS             complement(1437..>2222)
                     /locus_tag="PLX85_07385"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC63029.1"
                     /translation="ATVAGNGAQIWGRFAPTMFVGLVTLELVQLPLAWSLARALRHRQ
                     DERESLLRRAVTASERERRTIAGELHDGPVQDLLGQSYALIATADRHSDRVPDLVVRD
                     LHEMAARTRSTVQALRSLLIDLYPPNLHSEGLGAAIDDLAGPLRARGVTTVVDVEPST
                     PIPEHLEALLYRVSREALRNAQQHADASRVDVTLRTEGARVLLVVTDDGVGLPPEVRA
                     EQRAGGHLGLELIESLVRDAGGSASIGAVEGGGTRVVVEVPLA"
BASE COUNT          346 a          880 c          684 g          312 t
ORIGIN      
        1 cccccgccgc ggcgacggcc gccgcgacac ggcagtccac gcgcactccc acggcgtcca
       61 ccggctccgc cagttcgcag gctccgaggc gcgacctcgc ggccgacgaa gccgccggcg
      121 ggcataccct cgagcgccat gtcggacgca ccgatgaaca actccgccag cgcttgcgcg
      181 acgaacccga catttccgcc gcctccacct ataccgaccg tgacaccgcc gaacgagtgg
      241 taggcgcggc cctgcagcgc aaccaggcga agatcgcgga ctgggtgaag caagggcgca
      301 cccgcaccaa cctggcgctc gactacgaag ccggcgaggt catcggccgt tccatccccc
      361 gcggcaagac ggtcgcccac accgtcaccg ccgccacggt cgtcctgcgc gccgatggct
      421 cgagctggtt cgtcctcacg agctaccccg atgactagcg gcggcccacc cgggaccgac
      481 agcctgcgca agatgctgcg cggctacttc caccaggacg tcatcgccga gcatgggagc
      541 atcgaggccg ccgcccgcgc ctaccgcgcg gaagccccac ccgcggaacg ctggcgggcc
      601 gcccgccagc tccgggtgct gctcgcaggc tgcgaaacgg tcgaggacgt ccgcgacgcc
      661 ttcgcagccc tgcgcagcgg ctggcgtcct cgcgacctgc gctccgtcga gcgcgtcctt
      721 gccatcctcg aaacggacgg ctcggtaagc tgagcgtccg tcagcgcgcc gagcccggcg
      781 cccgccctcg gctccggtcc cgactcaccg caccagccct cgtcgctccg cccacaacgc
      841 cgcctgagtc cggtcggaca cgccgatgcg gtcgaacacg ttcacgaggt gggccttgac
      901 cgtcttttcg ctgatcccga gctcacgcgc gatctgctta ttcagcaggc cgcgggcgag
      961 cagcaccagc acctcgcgtt cgcgtggcgt gagctgccgc ccggcctcgc gcacccggcg
     1021 ctcctcgagc agcgcgttcg ccaccttcgg cgagagcggt gactcgcctc gcgctgccgc
     1081 ctcgaccccc cggatcagct cagccggctc ggcgtctttg agcaggtagc ccactgcccc
     1141 cgcgtcgagc gcgtcgagga tacgactgcg gtccgaaaac gacgtcagca cgatcacccg
     1201 cgcgcttggt gctgtctcct gcagacggcg agtcgcttcg acgccatcga cgcccggcat
     1261 ggcgagatcc atgagcacca ccgccggttg cagctcaccg gccagccgca ccgcgctctc
     1321 gccatcgccg gccgttccga ccacttcgat gttcgcctgg ccgctcagaa attggctgat
     1381 gccaatccgc accagctcgt ggtcatccac gatgacaact cggatcggaa cgtcggtcac
     1441 gcgagcggca cctcgacgac cacgcgcgtc ccgccccctt ccaccgcccc gatcgaggcg
     1501 gatccacccg catcccggac gaggctctcg atcaactcca ggccgaggtg cccccccgcc
     1561 cgctgttcag cgcggacctc aggcggcaat cctacgccgt catcggtgac gacgagcaga
     1621 acgcgggcgc cctccgtccg caacgtcacg tccacgcgcg acgcgtcggc gtgttgctgg
     1681 gcattccgca gcgcctcgcg actcacgcgg tacagcagag cctcgaggtg ttccgggatc
     1741 ggcgtgctcg gttccacgtc taccaccgtc gtgactcccc gcgcccgcaa cgggccagcg
     1801 aggtcgtcga tcgccgcccc caggccttcg ctgtgcaggt ttggcgggta caggtcgatc
     1861 aggagggaac gtagagcctg caccgttgag cgcgtgcgtg ccgccatctc gtgcaagtcg
     1921 cgcaccacga ggtcaggaac acggtccgag tgtcggtcgg ccgtcgcaat caacgcgtac
     1981 gactgcccca gcaggtcctg caccggccca tcgtgcaact cgcctgcgat cgttcgccgt
     2041 tcccgctccg aagccgttac ggcgcggcgc agcaacgatt cccgttcatc ctgccgatgc
     2101 cggagcgccc gcgcgagcga ccacgccagc ggcagctgca ccagctcgag cgtcacgagg
     2161 ccgacgaaca tcgtcggggc gaagcgtccc cagatctgtg cgccgttgcc agccaccgtc
     2221 gc
//
LOCUS       DAORPG010000329         2219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5904_length_2219_cov_3.653420, whole genome shotgun sequence.
ACCESSION   DAORPG010000329 DAORPG010000000
VERSION     DAORPG010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5904_length_2219_cov_3.653420"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLX85_07390"
     CDS             complement(<1..292)
                     /locus_tag="PLX85_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRC63030.1"
                     /translation="MPELRAGRLLVATPILDDPNFRRTVVLLCSHDEHGSFGLVLNRP
                     LEATVADLAPEWGRLAAPPPVLFAGGPVDRVQAFALARRDQAAEESWWTAILE"
     gene            383..688
                     /locus_tag="PLX85_07395"
     CDS             383..688
                     /locus_tag="PLX85_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63031.1"
                     /translation="MTRTEVGFPVPVLQLIAFIVSVPFLLLFVLLASTAFRGGERAGY
                     HQPADTSADGEDGSRKRRRTRIHPINQLFVWGLALTLFAATIAMLVASVVYDSWSWA"
     gene            731..874
                     /locus_tag="PLX85_07400"
     CDS             731..874
                     /locus_tag="PLX85_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63032.1"
                     /translation="MATYRLTTRVGACVHCGTATVQSCAGCERFVCRDCEWQHHPTPS
                     EHQ"
     gene            complement(909..1541)
                     /locus_tag="PLX85_07405"
     CDS             complement(909..1541)
                     /locus_tag="PLX85_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015167142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRC63033.1"
                     /translation="MHLDLIEIVKWAGYVGLFVIIFAETGLLFGFFLPGDSLIITAGV
                     LATDKFPDHQLSLAILIPLLIVAAVTGDATGYLIGKQMGPRLFVRDDARFFRRKHLVR
                     ASEFYEKHGGKTIVLARFLAIIRTFAPTVAGAAGMPWGTFTFFNVIGGASWVVSMLLL
                     GYFVGEAIPNLDAFFFGILAVVIGISLAPAVWHLWRERSASRRARSSTND"
     gene            1607..>2219
                     /locus_tag="PLX85_07410"
     CDS             1607..>2219
                     /locus_tag="PLX85_07410"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC63034.1"
                     /translation="MLAARVRADDARETTASTRGATTVDAAPPSSASGPPPAAPSVRE
                     APSRGRFGVLGTTLSIAAGVGIASLSTYFWIPFLPLYAKELGARNEANAVFWVAVGST
                     TLGLGRVISGPAWGLAADRFGRKLMFVRALFFASITTLILIFAREPWHIAVAFACQGL
                     FSGYIPAAVALTTVSVPDTQMSRSLGIVNGAQYLGSTVGPAIGA"
BASE COUNT          356 a          792 c          733 g          338 t
ORIGIN      
        1 cttcgaggat cgccgtccac caggattcct cggcagcctg gtcgcgccgc gccagcgcga
       61 acgcctgcac gcgatcgacc ggcccaccgg cgaacagcac aggcggcggt gccgccaacc
      121 gtccccactc gggggcaagg tcggccaccg tcgcctcgag cggccggttg agcaccagac
      181 cgaaggatcc gtgctcgtcg tgggagcaca gcagtacgac cgtccgacgg aagttcgggt
      241 cgtcgaggat gggcgtcgca accagcagac gaccagctcg cagctcgggc atgctcgctc
      301 ctcaacgcgt caccgagcat agcccgaggc acgaacgacg gcttcaccgt gtaccgaagc
      361 gtggatatga tgcgggcgcg cggtgacgcg gacggaagtg gggtttcccg tgccggtgct
      421 tcagctgatc gccttcattg tctcggtgcc gttcctgctg ctgttcgtgc tgctcgcgag
      481 caccgccttt cgaggcggcg agcgggccgg ctaccaccag ccggcggata cgtcagcgga
      541 cggcgaggac ggctcgcgca agcgccgccg cacgaggatt catcccatca accagctctt
      601 cgtctggggc ctcgcgctga cgctgttcgc cgcgaccatc gcgatgctcg tggcctcggt
      661 cgtctatgac agctggtcct gggcctgacg cgagcgcgcg gcgcacgggc gcctgaggta
      721 ccatcgagtc atggccacct accgtctcac cacccgcgtc ggcgcctgcg tgcactgcgg
      781 caccgctacc gtccagtcat gcgccggctg cgagcgcttc gtgtgccgtg actgcgagtg
      841 gcagcaccac cccaccccct cggagcacca gtagccggct cgggtcccgg cccggagcat
      901 cctcgcagct agtcgttcgt ggatgaacgc gcccggcggc tggctgagcg ctcgcgccag
      961 aggtgccaca cggccggagc gagcgagatg ccgatgacca ccgcaaggat gccgaagaag
     1021 aaggcgtcga ggttcgggat cgcctcgccg acgaagtagc cgagcagcag catcgagacg
     1081 acccagctcg cgcctccaat cacgttgaag aacgtgaacg tgccccacgg catgcctgcc
     1141 gcgcccgcca ccgtgggagc gaaggtgcga atgatggcga ggaagcgagc gagcacgatc
     1201 gtcttgccgc catgcttctc gtagaactcg ctcgcgcgga ccaagtgctt gcgacggaag
     1261 aagcgtgcgt cgtcgcggac gaagagccgc ggccccatct gcttcccgat caggtagccg
     1321 gtcgcgtccc ccgttacggc cgccacgatc aggagcggga tcaggatggc cagcgagagc
     1381 tggtggtcgg ggaacttgtc cgtcgccagc acgcccgccg tgataatcag tgagtctccg
     1441 ggcaggaaga agccgaacag cagcccggtc tccgcaaaga tgatcacaaa caggccgacg
     1501 tagcccgccc acttcacgat ctcgatcagg tcgaggtgca cgcgttcctc ctccagagcg
     1561 gcgggacctt cacagcgtac cccgcgcccc tcatgagcgt ctgtccgtgc ttgcggcacg
     1621 cgtccgggcg gacgatgctc gcgagacgac cgccagcacc cgaggagcca ccaccgtcga
     1681 cgccgcgccc ccgtcttcgg catccgggcc gccgccagcg gcgccgtccg tgcgcgaagc
     1741 gccatctcga gggcgcttcg gcgttctcgg cacgaccctg agcatcgccg ccggcgtcgg
     1801 catcgccagt ctctcgacct acttctggat cccgttcctg ccgctgtacg cgaaggagct
     1861 cggcgcgcgc aacgaggcga acgccgtgtt ctgggtcgcg gtcggaagca ccacgctcgg
     1921 actggggcgc gtcatcagcg gcccggcctg gggcctcgcc gccgaccggt tcgggcgcaa
     1981 gctcatgttc gtgcgggccc tgttcttcgc gagcatcacg acgctcatcc tcatcttcgc
     2041 ccgcgagccc tggcatatcg cggtcgcctt cgcatgccag gggctgttct cgggctacat
     2101 cccggcggcc gtggcgctca ccaccgtcag cgtcccggac acccagatga gccgttcgct
     2161 gggcatcgtg aacggcgcac agtacctcgg cagtacggtc ggcccggcca ttggcgcgg
//
LOCUS       DAORPG010000330         2214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5932_length_2214_cov_4.216767, whole genome shotgun sequence.
ACCESSION   DAORPG010000330 DAORPG010000000
VERSION     DAORPG010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5932_length_2214_cov_4.216767"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..447)
                     /locus_tag="PLX85_07415"
     CDS             complement(88..447)
                     /locus_tag="PLX85_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRC63035.1"
                     /translation="MRVVDGDTLIVAASEGGSERVRVFGLDAPEASERCGPEATRELE
                     QATGSQVRLLTDRRTRDQFGRELRYLFTPDGRSIDADLVRHGSARAWREDGAFRDILV
                     GIENAVRVEGVGCLWQG"
     gene            complement(649..1227)
                     /locus_tag="PLX85_07420"
     CDS             complement(649..1227)
                     /locus_tag="PLX85_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63036.1"
                     /translation="MAAMSSLLHLVVASLADPLDRSAFERAVEFARSLCQAPGVRQQS
                     IGWSDHQLVVATILEASDDLDQFVASEQHMRFVMHGLAPIISGMWSTAIEAGNATTAP
                     DAARLWAFALPEREGVYEWQVRQFLDDAASLGDGAIVGVTIEERERFRAGGLVSFNAA
                     TEDVAERTAAARRRWAALGGELEDALVDAHRP"
     gene            1290..>2214
                     /locus_tag="PLX85_07425"
     CDS             1290..>2214
                     /locus_tag="PLX85_07425"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HRC63037.1"
                     /translation="MPSNDVEKPLLVILDSHGIIYRSYFALRDVLTVRRTGEPVAAVY
                     GYANSLLYVINELHPRYIMAAWDGPQPTFRHEADETYKATRVSMPDDLRPQVGYVRKL
                     LDAFRIPVIEAPGYEADDVLGTLAAQGRDAGLDVVIVTLDNDIVQLIQPGVRVYMFRP
                     YQRDYVLYDEERVRDRWQFEPRQMVDYKALVGDTSDNIPGVRGIGEKGAVALIQQWGT
                     VEAMLDHLDEVKPPRAQKALSEGRDIALHSKHLATIVCDVPGVQLDLERAAVRDYDRD
                     GVLELFRELEFRSLVSRLPEHVTDVPLPERPP"
BASE COUNT          378 a          760 c          726 g          350 t
ORIGIN      
        1 ggcgcgcatg gtgatcgccc cgtcgatcaa gaccgagggc aagatctacc agatggcgca
       61 gcgcgtgcgg gactgatcat cgagggatca tccctgccag agacagccga ctccctcgac
      121 gcggactgcg ttctcgatgc caacgaggat gtcacggaac gcgccgtcct cgcgccaggc
      181 gcgggcgcta ccatggcgta ctaagtcggc gtcgatcgag cgcccgtcgg gcgtgaacag
      241 gtaacgcagt tcgcgcccga actggtcgcg tgtccgtcgg tcagtgagca ggcgcacctg
      301 cgatcccgtc gcctgctcaa gttcgcgcgt cgcctcgggg ccgcagcgct cgctcgcctc
      361 gggagcgtcc agcccgaaca cgcggacacg ctcgctgccg ccttccgagg cggccacgat
      421 gagcgtgtct ccatccacga cacgcacgac gcgagcgagt gggagccgct cgggctcatc
      481 cagccaccac gaaccatcgc ctcgccgcac gagttgggca tcggccgcgg gccgcggtgt
      541 cgcggtggat gcaggcggtg cggtcgcggc tccggcgcat ctcgacgcga tgaccaagag
      601 cgtgaggagc aggaacgccc gggccgccca cgtcacgcgc aacacggttc aggggcggtg
      661 tgcgtcgacg agtgcgtctt cgagttcacc accgagcgct gcccatcgtc gccgcgccgc
      721 ggccgtccgc tccgcaacgt cctcggtcgc ggcattgaag gacacgaggc ccccggcgcg
      781 gaatcgctca cgctcttcga tcgttacacc cacgattgcg ccgtcaccga ggctcgcggc
      841 gtcgtcgagg aactggcgga cctgccactc gtagacgcct tcccgctcgg gaagggcgaa
      901 cgcccagagc cgcgcggcat ccggggccgt cgtcgcgttg ccggcctcga tggctgtact
      961 ccacatcccg ctgatgatcg gcgcgaggcc gtgcatgacg aaacgcatgt gttgttcgga
     1021 ggccacaaac tggtcgaggt cgtcggaggc ctcgagtatg gtcgcaacga cgagctggtg
     1081 gtccgaccag ccgatcgact gttgccggac gcccggcgcc tggcaaaggg atcgcgcgaa
     1141 ctcgacggcc cgctcgaacg cactacggtc gagtggatcg gcgaggctcg cgacgacgag
     1201 gtgcagcaac gaactcatcg cagccatcgt agaacctcgt cccatcagtc gcagacggcg
     1261 gagccctggc tcgccgctat actgcggtga tgccatcgaa cgacgtcgaa aagccgctgc
     1321 ttgtcatcct cgattcgcac ggcatcatct accgctcgta tttcgcgctg cgcgacgtcc
     1381 tcacggtccg ccgcacgggc gagccggtcg cggcggtgta cggctacgcg aacagcctcc
     1441 tgtacgtgat caacgaactg catcctcggt acatcatggc cgcgtgggac gggccgcaac
     1501 ccacatttcg gcacgaggcc gatgagacct acaaggccac ccgagtttcc atgcccgacg
     1561 acctgcgtcc gcaggtgggc tacgtacgaa agctcctcga tgccttccgc atcccagtca
     1621 tcgaggcccc aggctacgag gccgatgacg tcctcggtac gctggccgca cagggccgcg
     1681 acgcgggact cgacgtcgtg atcgtgacgc tggacaacga catcgtgcag ctgattcagc
     1741 ccggcgtgcg cgtctacatg ttccgcccct accagcgaga ctacgtgctc tacgacgagg
     1801 agcgggtgcg cgaccgctgg cagttcgagc cgcgccagat ggtggactac aaagcgctcg
     1861 tcggagacac ctcggacaac atcccgggcg tgcgtggcat cggtgagaag ggtgctgtgg
     1921 cgctcatcca gcagtggggc acggtcgagg cgatgctcga ccacctcgat gaggtcaagc
     1981 caccgcgagc gcagaaggcc ctttccgagg ggagagacat tgctctccat tccaagcacc
     2041 tggcgacgat cgtgtgtgac gttccgggcg tgcagctcga tctcgagcgg gcggctgttc
     2101 gcgactacga ccgtgacggc gtactcgagc tcttccgcga actcgagttc cgttcgctcg
     2161 tcagccggct gccggagcac gtcaccgatg tgccgctccc ggagcgtccg cccc
//
LOCUS       DAORPG010000331         2214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5934_length_2214_cov_3.121352, whole genome shotgun sequence.
ACCESSION   DAORPG010000331 DAORPG010000000
VERSION     DAORPG010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5934_length_2214_cov_3.121352"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1184)
                     /locus_tag="PLX85_07430"
     CDS             complement(12..1184)
                     /locus_tag="PLX85_07430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRC63038.1"
                     /translation="MKVALVSPYDWLTPGGVNQHVDQLAKQLVMRGHEVRILAPATGP
                     VDDPRVTVAGTPVPVPASGSRARITFDPRLGRLVRRFLEAEQFDVVHLHEPLMPTLPL
                     QVLRHSRAVNPDVVNVGTFHAHSAGGNRMYSYWRIVLKRWFRELDGKIAVSPPAAEYH
                     HRYYPGYYNVIPNGIDVEHFASRDIEPLPELEDGSVNILYVGRAEKRKGLPYLLRAFG
                     TVNARNPNTRLIIVSRDSRRLRRYRDSIERQGRRGIVFADDVSNADLPRYHRSSQVFC
                     SPATGNESFGYVVVEAMAAGLPVVASNIEGYAYVMTHGVEGLLVPPEDTFALADALTA
                     LVRDHDARAAMGAAGYKRANEFSWPRVTQQVLSYYERLRYARGATPRADERARWHA"
     gene            complement(1181..1981)
                     /locus_tag="PLX85_07435"
     CDS             complement(1181..1981)
                     /locus_tag="PLX85_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HRC63039.1"
                     /translation="MTAESNRAHTDSLGPRRFGTADLQIHTSYGDGMADARELLARIE
                     ERTDLDIIAVTDHDDVGGALLAREVHARGNYSFEFVPGVELTTRAGHLLALWVDEPLR
                     PLRPLGETVATIHRMGGVAVIPHPFSYLTRSVGQRALERLIAEGDPETLPDGIEVANL
                     TLAGRVTGAKALRLNRERYGLAETGGSDAHFPEEVGTARTVFAGTTAAELRAAILART
                     TTGLRAHQVGLRTIGVRRLAAQQVRGLSVTPKKVLGPHVRRLAGAVRR"
BASE COUNT          326 a          801 c          762 g          325 t
ORIGIN      
        1 cttcatcgac gtcacgcgtg ccatcgcgcg cgctcgtcgg cgcgcggggt ggcgccgcgg
       61 gcgtagcgga gccgctcgta gtacgagagc acctgctgcg tcacccgcgg ccacgagaac
      121 tcgttcgctc gcttgtaccc cgccgcgccc atcgcggctc gcgcgtcgtg gtcacgcacc
      181 agcgcggtca acgcgtccgc gagcgcgaac gtgtcctcgg gcggcacgag caggccctcg
      241 acgccatgcg tcatcacgta ggcgtacccc tcgatgttcg aggccaccac cggcagcccc
      301 gccgccatcg cctcgacgac gacgtagccg aaggactcgt tgccggtggc gggcgagcag
      361 aagacctgcg agctgcggtg gtaacgaggc aggtcggcgt tcgacacgtc gtccgcgaac
      421 acgatgccgc gccgcccctg ccgctcgatc gagtcacggt agcgccggag ccgcctcgaa
      481 tcgcggctga cgatgatcag acgggtgttc gggttacgcg cgttgaccgt cccgaaggcc
      541 cgcagcaggt agggcagtcc ctttcgcttc tcggcccggc caacgtagag gatgttgacc
      601 gagccatcct cgagctccgg cagcggctcg atgtcgcggc tcgcgaagtg ctcgacgtcg
      661 atgccgttcg ggatcacgtt gtagtaaccg gggtagtagc ggtggtggta ctccgccgcc
      721 ggcggggaca ccgcgatctt gccgtccagc tcgcggaacc agcgcttcag gacgatgcgc
      781 cagtacgagt acatgcggtt gccgcccgcg ctgtgcgcgt ggaatgtccc cacattcacc
      841 acgtccgggt tcacggcgcg cgaatggcgc agcacctgca acggcagcgt cggcatgagc
      901 ggctcgtgca ggtgcactac atcgaactgt tccgcctcga ggaagcgccg cacgagacga
      961 cccagccgcg gatcgaacgt gatgcgagcg cgcgaccccg aggcaggaac cggcacaggt
     1021 gtccccgcga ccgtgacgcg cgggtcgtcg accggccccg tcgcgggcgc gaggatgcgc
     1081 acctcgtggc cgcgcatgac gagttgcttc gcgagctggt cgacgtgctg gtttaccccc
     1141 cctggcgtca gccagtcgta gggcgaaacc agggcgacct tcatcggcgc accgcgccgg
     1201 ccaaccgccg aacatgcgga cccaggacct tcttcggggt cacggagagc ccgcgcacct
     1261 gttgcgccgc gagccggcgc acaccgatcg tgcgcaggcc cacctggtgc gcgcgcagac
     1321 ccgttgtcgt ccgcgcgagg atggccgcgc gcagctcggc agccgtcgtc ccggcgaaca
     1381 ccgtccgagc cgtgccgacc tcctcgggga agtgggcgtc gctgcccccc gtctccgcga
     1441 ggccgtagcg ctcgcgattc aaccgcaacg ccttcgcccc cgtcactcgg ccggccagcg
     1501 tcaggttcgc cacctcgatg ccgtccggca gcgtctccgg gtcgccctcc gcgatcaggc
     1561 gctcgagcgc gcgctgcccc accgagcgcg tcaggtacga gaacggatgc ggtatcaccg
     1621 cgactccgcc catacggtga atggtcgcga cggtctcgcc gaggggccgc agcggccgca
     1681 gcggctcatc gacccagagc gcgaggaggt ggcccgcccg cgtcgtcagc tcgacgccgg
     1741 ggacgaactc gaaggagtag ttgccgcgcg catgtacctc gcgcgcgagg agcgccccgc
     1801 ccacgtcgtc atggtcggtc acggcgatga tgtcgaggtc ggtccgctcc tcgatgcgtg
     1861 cgaggagttc gcgggcgtcc gccatgccgt cgccgtagga cgtatggatc tggaggtcgg
     1921 cggtcccgaa gcgacgtggc ccgaggctgt ccgtatgcgc gcgattcgac tcagcggtca
     1981 tcgaagccat tgtacgggcg catcggggcg cggagcaccg cggccccctc ggcgggcgct
     2041 cagagcgcaa gtccgctcca caccggctgc aacgggaacc actggtccgg cgcgcggcga
     2101 atcccggtct cgagcgccgc ggccacctgt cgcatccccg cctcgatgtc cgcctcgtgg
     2161 tcgccggtcc gctgcacctg gaatgcctcg tcgatgtaca cgcggtagcg cccg
//
LOCUS       DAORPG010000332         2213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5938_length_2213_cov_3.757646, whole genome shotgun sequence.
ACCESSION   DAORPG010000332 DAORPG010000000
VERSION     DAORPG010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5938_length_2213_cov_3.757646"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLX85_07440"
     CDS             complement(<1..391)
                     /locus_tag="PLX85_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63040.1"
                     /translation="MTESTGAVVAFDGMAASWSAPIESGQIAQAVCVGWGGDNHTEPA
                     TSDGNVALGCFSIAGIGTGSVTVTSLDSEGCIGNHIDVIYGVAPTPTPTPTPTPTSTP
                     SPTPTPPPAQCSDATLTVAVGGASQATY"
     gene            805..>2213
                     /locus_tag="PLX85_07445"
     CDS             805..>2213
                     /locus_tag="PLX85_07445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HRC63041.1"
                     /translation="MKRFEYKWVVAVVMVIGLFMELLDMTIVNVALPGFARDFEASAT
                     TIQWIVTGYLLSLAVVIPLSGWAGDRYGTKRTFMFALTVFTSGSLLCALAQNAEQLIA
                     FRVVQGVGGGMLTPVGTAMLFRAFPPNERSKASALLAIPASMAPAMGPVVGGYLVEYH
                     SWHWIFLINIPIGLAGLAISALYLDEHRELAGKRLDVPGFVLSAGGLAALIYGLSEAG
                     DRGFEDPRALGFMVGGLVTLTVFVLLELRTAQPMLNVRLFTNRLFATNNAVQFVAMAG
                     FSGVLFILPLLLQAERGLTPLQSGLTTFPMALGVLTFAPFVGRIYPRVGPRRLMVAGL
                     IVGSLSAFVFVWIDLETSQWWIRAIMYGRGVGFALMLVPLQAATFATISNRDTGEASA
                     MFNVGRQVASSFGVALLATVLTRRLVEHGGQLGNPATRDASLSAFHDAFVVSGLIIVI
                     GVAAAFLVRDHEAAASMQRT"
BASE COUNT          340 a          730 c          750 g          393 t
ORIGIN      
        1 agtaggtcgc ctgcgaagca ccaccgaccg cgacggtcag cgtggcatcc gagcactgcg
       61 cgggcggcgg cgtcggggtc ggtgagggtg tggaggttgg cgtcggggtc ggggtggggg
      121 tcggtgtcgg cgctacgcca tagataacgt cgatgtggtt cccgatgcac ccctcagaat
      181 cgagcgaggt gaccgtgaca ctcccggtac cgataccggc gatcgagaag caaccaaggg
      241 ctacgttgcc gtcagacgtg gcaggttcgg tgtggttgtc tcctccccat ccgacgcaga
      301 cagcctgggc gatctggcca gactcgatcg gagcggacca ggaagcggcc atgccatcaa
      361 acgcgaccac cgcgccggtc gactcggtca cgttgcaagc ctcggggctt cccgggccgg
      421 taccgtagac cgtgcgcgga ccgacgagca gcgggtacgc ggcgagggcc gcggaggcca
      481 ggaccaccgc gagcagaagc cagcgcgtgg gctgaccgga acggatgagc cggatccgag
      541 gcactcgtcc tcccgcctcg cgcgcccccc gcgatgacgc tcgcaaccgc gagaagatat
      601 cccaactgaa gcgcgtgcga aatcccgcgg ccggaacgtg ccaaacgggc atgcactcga
      661 aacgtcgcgg cctgcgggac acggtacgct ggtttgacgt gccccggccc ataaggtccc
      721 gcccgaacgg gcaggttgac cagcttcgag gccgggcagg atgctgggat ctcaactgac
      781 cattggtcag taaggaggca gccaatgaag cgttttgagt acaaatgggt cgtcgccgtc
      841 gtcatggtca tcgggctctt catggagctg ctcgacatga ccatcgtcaa cgtcgcgctg
      901 cccggcttcg cccgcgactt tgaggcctca gcgacgacca tccagtggat cgtgaccggg
      961 tacctgctct cgctcgccgt cgtgatcccg ctcagcggat gggccggcga tcgttatggg
     1021 accaagcgga cgttcatgtt cgcgctcacg gtcttcacca gcggttccct gctctgtgca
     1081 ctcgcacaaa acgccgagca gctgatcgcg ttccgcgtgg ttcagggcgt gggtggtggg
     1141 atgctcaccc cggtcgggac ggcgatgctc ttccgcgcct ttccgcccaa cgaacgatcg
     1201 aaggcctcgg ccctcctcgc gatccccgcc tcgatggcgc cggcgatggg gccggttgtc
     1261 ggcggctacc tggtcgagta ccactcgtgg cactggatct tcctgatcaa catcccgatc
     1321 ggtctggccg gcctcgccat ctcagcgctg tatctcgacg agcatcgcga gctggcgggt
     1381 aagcgcctcg acgtgccggg cttcgtgctg tcggccggcg ggctcgccgc gctcatctac
     1441 ggcctgtccg aggccggcga ccgcggcttc gaggacccgc gcgccctggg cttcatggtc
     1501 ggcggcctgg tgacactcac ggtcttcgtg ctgctggagc tgcggacggc gcaaccgatg
     1561 ctgaacgtcc gcctcttcac caaccggctg ttcgcgacca acaacgccgt acagttcgtg
     1621 gcgatggctg gcttcagcgg cgtgctcttc atcctgccgc tgctcttgca ggcggaacga
     1681 gggctcacac ccctgcagtc gggtctgacc acgttcccga tggccctcgg cgtgctcacg
     1741 ttcgcgccgt tcgtcgggcg catttacccg cgcgtcgggc ctcggcgact gatggtggcg
     1801 ggactgatcg tcggttcgct ctcggcgttc gtgttcgtct ggatcgacct cgagacgagc
     1861 caatggtgga tccgggcgat catgtacgga cgcggggtgg gcttcgcact gatgctggtg
     1921 ccgttgcagg cagccacgtt cgcaacgatc agcaaccgag acactggcga ggcaagcgcg
     1981 atgttcaacg tcggccgtca ggtcgcctcg agcttcggcg tggcgctgct cgcgaccgtc
     2041 ctgacaaggc ggctcgtcga gcacggcggg cagctcggca acccggctac gcgcgacgcc
     2101 tcgttgtccg ccttccatga cgcgttcgtg gtgagcggcc tgatcatcgt catcggcgtc
     2161 gcggcggcgt tcctcgtgcg cgaccacgag gcggctgctt caatgcagcg cac
//
LOCUS       DAORPG010000333         2211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5951_length_2211_cov_3.640538, whole genome shotgun sequence.
ACCESSION   DAORPG010000333 DAORPG010000000
VERSION     DAORPG010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5951_length_2211_cov_3.640538"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLX85_07450"
     CDS             complement(<1..315)
                     /locus_tag="PLX85_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63042.1"
                     /translation="MVTPIAPASMYRTREGLGLWEHKGKVAAVGVGHSKTARRWDGTA
                     ENCIGAWAIEAMRKAIEDAGISPSEVDGIVLDTTTTTGAHWPAGRPIPTEVVNAYKPT
                     DDP"
     gene            complement(358..771)
                     /locus_tag="PLX85_07455"
     CDS             complement(358..771)
                     /locus_tag="PLX85_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HRC63043.1"
                     /translation="MPKQSPVPEDVDRGFFEAANQDRLVIQNCTACNRLQHPPQPACG
                     ACGSADHLEWKEMSGKGTIYSYAVVYDSPVASLQADQPYNCAIIALDEDPGIKMLSHL
                     PGTPVDEVPMDAPVRAIFEVTQATGQKVVEWQVVN"
     gene            966..1142
                     /locus_tag="PLX85_07460"
     CDS             966..1142
                     /locus_tag="PLX85_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63044.1"
                     /translation="MNSWSVDLVTVQVARDECARCGHIEEAHVLEDVPGELEKREFCA
                     ECPDWHEFRSTELS"
     gene            1237..>2211
                     /locus_tag="PLX85_07465"
     CDS             1237..>2211
                     /locus_tag="PLX85_07465"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRC63045.1"
                     /translation="MSEHPLDPVFHPRAIALVGVPERLDGRGADFFLALLDQGFHERK
                     PVYIVHPRASEIRGIKAYPTLLDCPDPVDHVISLIPRHLVSTLVDQCVEKGVRSLHFF
                     TAGFSESGEEGMAAAERELAARARAGGVRVLGPNCMGLYVPGERISFSLGFPTDAGNV
                     MMISQSGANAGGVVSGLAPRGLRFSKVVSFGNGSDIDAAELFEYAGSDVETELVLAYI
                     EGVPDGRRFLEAVRRCARVKPTIILKGGFTDAGARAASSHTGSLAGSAAIFEAMCRQT
                     GALRVDSLDELFDMAVAVTTRARQIRGRRAVLTGGGGGFSVLSADAIAH"
BASE COUNT          299 a          758 c          776 g          378 t
ORIGIN      
        1 cgggtcgtcg gtgggcttgt aggcgttcac gacctcggtc gggatcgggc ggcccgccgg
       61 ccagtgggcg ccggtcgtgg tcgtcgtgtc gaggacgatg ccgtcgacct cgctgggcga
      121 gatgccggcg tcctcgatcg ccttgcgcat ggcctcaatg gcccacgcgc cgatgcagtt
      181 ctcggcggtg ccatcccagc ggcgagcggt ctttgagtgg ccgacgccga cggcggccac
      241 cttccccttg tgctcccaga gcccgagccc ttcgcgcgtc cggtacatcg acgccggagc
      301 gatcggagta accatcatgt ccctcttttc cccgccgtca ggcgggccgg gtcgacgcta
      361 gttgacgacc tgccactcga cgaccttctg gccggtcgcc tgcgtcacct cgaagatggc
      421 gcgtacgggg gcgtccatgg ggacctcgtc gaccggcgtg ccggggaggt gcgagagcat
      481 cttgatgccc gggtcctcat ccagcgcgat gatcgcgcag ttgtacggct ggtcggcctg
      541 gagcgacgcg accggcgagt cgtagacgac ggcgtagctg tagatcgtgc ccttgccgct
      601 catctctttc cactcgaggt ggtcggccga gccgcaagcg ccgcacgccg gctgaggcgg
      661 gtgctgcagg cggttgcagg ccgtgcagtt ctggatcacg agccggtcct ggttggccgc
      721 ctcgaagaag cccctgtcca catcctccgg aactggtgac tgtttgggca tgtctccccc
      781 tcgttcttct cactgcgccc gtttgctgtg gcccgtatgg tgccgtgcac attcggcgac
      841 gggaaccctg catcgatgta gcatccccat ctccgaatgt caaaggtgat gggtcgccgt
      901 ggtgatcgcc cgtgcgcgac cgccgggccg gggccccaac gggcgggagt tgctacgata
      961 cggtcgtgaa cagctggtcg gtcgatctcg tcacggtgca ggtcgcgcgt gacgaatgcg
     1021 cgcgctgtgg ccacatcgag gaggcgcacg tcctcgagga cgtccccggt gagctcgaga
     1081 agcgcgagtt ctgcgcggag tgtcccgact ggcacgagtt cagatcgact gaactgagct
     1141 gagcccgacc gcggccggac gcgtcccgcc tcgacgcagg tggcgtcgct ggggcgtccg
     1201 cgcgactgcc ggcggaggaa cgaaggacgt ggcgacgtga gcgagcatcc actggacccg
     1261 gtctttcatc cgcgcgcgat cgcgctcgtc ggggtgccgg agcggctcga cggccgtggc
     1321 gcggacttct tcctcgcgct gctcgaccag gggttccatg agcgcaaacc ggtttacatc
     1381 gtgcacccgc gggcgagcga gatccggggc atcaaggcct acccgacgct gctcgactgc
     1441 cccgaccccg ttgaccacgt gatctcgctg atcccgcgtc acctcgtctc cacgctcgtc
     1501 gaccagtgcg tcgagaaggg cgtgcgctcc ctgcacttct tcaccgccgg cttctccgag
     1561 agcggcgaag agggcatggc agccgcggag cgtgaactcg cggcccgggc gcgggcaggc
     1621 ggcgtacgcg tcctgggccc caactgcatg gggctctacg tgcccgggga acgcatctcg
     1681 ttcagcctcg gcttcccgac cgacgcgggc aacgtgatga tgatctccca gtccggcgcg
     1741 aacgcgggcg gtgtggtcag cgggctggcg ccgcgcgggc tgcgcttctc gaaagtcgtc
     1801 tccttcggca acgggtccga catcgacgcc gccgagctat tcgaatacgc gggcagcgac
     1861 gttgagaccg agctcgtgct cgcgtacatc gagggcgttc ccgacggccg ccgcttcctc
     1921 gaggccgtcc ggcgctgcgc ccgcgtcaag cccacgatca tcctcaaggg cggattcacc
     1981 gacgccgggg cgcgggccgc gagctcgcac accgggtcgc tcgctggctc cgccgcgatc
     2041 ttcgaagcga tgtgccggca gaccggcgcg ctgcgcgtcg actcgctcga cgagctgttc
     2101 gacatggccg tggcggtaac gacgcgcgcg cggcagatcc gcggccggcg cgctgtgctc
     2161 accgggggcg gcggcggctt ctccgtgctc tcggcggacg cgatcgcgca c
//
LOCUS       DAORPG010000334         2210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5957_length_2210_cov_3.639907, whole genome shotgun sequence.
ACCESSION   DAORPG010000334 DAORPG010000000
VERSION     DAORPG010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5957_length_2210_cov_3.639907"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1529)
                     /locus_tag="PLX85_07470"
     CDS             complement(15..1529)
                     /locus_tag="PLX85_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HRC63046.1"
                     /translation="MTANPVFNLARDSIGNRAASPESGSRPAFTFISDEGVRSWTYAE
                     AWAEIERYAQGLLARDLRRGERVLIRLPHSAEYAFAFFGANLAGLVPVPASPQLTEEE
                     VDFLLSDSGASGIIAWPAEQPSRASCPRFSPEELRHELRGMELPETHAEDPAYLIYTS
                     GTTAKPKGVLHAQRVLIGRSFMVGDAWESLSASDVVMHAGALNWSYTMGVGLMDPWSV
                     GARAILVASGTEPASWPRLIEAHGVTVFAAVPTVYRQMLKYGRPEDHDLSRFRFGLCA
                     GEALPPETAQMWRERVGTELYEALGMTECSTYISSGPGIPTKSGSPGKPQPGRRVAIL
                     SEETGGALPVDAVGLLAIHRSDPGLMLGYWRRPDEEAAVFRGEWFVGGDRASLDDDGY
                     VWFHGRADDLIKSFGYRLSPIEIEAALSTCAGVAEVAVVGLPLDADKTLVTACIVAED
                     GVDLTDRELAEHAARHLAGYKRPHQYRFVDSFPRTANGKIRRSELVRDLLAEVR"
     gene            complement(1526..1852)
                     /locus_tag="PLX85_07475"
     CDS             complement(1526..1852)
                     /locus_tag="PLX85_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HRC63047.1"
                     /translation="MPNETTTPDVTREQLQEPDEEHLYHLVLLNDDDHTYQYVIELLG
                     RILGYHPEKAFALACMVDSEGRATVETAQHEQVTRHQRQIHAFGPDPRVRRCRGSMSA
                     VVEPAA"
     gene            1903..>2210
                     /locus_tag="PLX85_07480"
     CDS             1903..>2210
                     /locus_tag="PLX85_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC63048.1"
                     /translation="MDVDVLVVGAGIVGLALAAELASDCSVVVVERHASYARETSSHN
                     SGVIHSGIYYQTGSWKHRLCLEGNAALYDWCARQGVTHQRLGKLIVATSRDDLEQLEA
                     "
BASE COUNT          389 a          736 c          708 g          377 t
ORIGIN      
        1 gccgaggcaa ccagctaacg gacttccgcc agcaagtccc gaacaagctc tgagcgccgg
       61 atcttgccgt tcgccgtccg tggaaatgag tccacgaacc gatactgatg cggccgtttg
      121 tagcccgcca ggtgccgcgc cgcatgctcg gccaactcgc ggtcggtcaa atccacgccg
      181 tcttccgcaa cgatgcaggc ggtgacgagt gtcttgtcgg cgtcgagtgg caggcccacg
      241 acggcaacct cggcgacccc cgcacacgtt gacagggccg cctcgatctc gatgggtgac
      301 agccggtacc cgaacgactt gatgaggtca tcggctcgtc cgtggaacca gacgtagcca
      361 tcgtcgtcca atgacgcgcg gtcgccgccc acgaaccact cgccccggaa cacggccgcc
      421 tcttcatcgg ggcgtcgcca gtatccgagc atcagtccgg ggtccgaccg atggattgca
      481 aggagaccga ccgcgtcgac tgggagcgcc ccgcccgttt cctcggacag gatggcgaca
      541 cgccggccgg gctgcggctt ccccggcgaa ccagacttcg tcgggatccc ggggccgctc
      601 gaaatgtacg tgctgcactc cgtcattccg agcgcctcgt acagctcggt cccgactcgt
      661 tcacgccaca tctgcgccgt ctcgggtggc aacgcctcgc ccgcgcagag tccgaaccga
      721 aaccgcgaca ggtcatggtc ttcgggccga ccgtacttca gcatctgccg gtacaccgtc
      781 ggaaccgccg cgaacacggt gacgccatgc gcctcgatca gccgaggcca cgaagccggt
      841 tcggtcccgc tggcaacgag gatcgcccgt gcacctacgc tccaggggtc catgaggccc
      901 acgcccatgg tgtacgacca gttgagcgct ccagcgtgca tcacgacatc ggatgcgctc
      961 aacgactccc acgcgtcccc gaccatgaac gatctgccga tgaggacgcg ctgtgcgtgc
     1021 aggacgcctt tgggcttcgc ggtggtgccc gaggtgtaga tgaggtacgc cggatcctca
     1081 gcgtgggttt ctggcagctc catgccgcgc agctcgtgtc gcagctcctc tggactgaac
     1141 cgtgggcatg acgccctcga gggctgttct gccggccatg cgatgatgcc gctcgcgccg
     1201 gagtccgaca gtaggaagtc gacctcctcc tcggtgagct gcggagaggc cgggaccggc
     1261 acgaggccgg ccagattcgc gccgaaaaac gcgaaggcgt attcggccga atgagggaga
     1321 cgaatcagga cgcgttcgcc acgtcgaaga tccctcgcga gcaggccctg ggcgtatcgc
     1381 tcgatctccg cccatgcctc ggcgtacgtc caggagcgaa cgccttcgtc cgagatgaac
     1441 gtgaacgctg gtctggaacc agactctggc gatgcggccc ggttcccgat gctgtctcgc
     1501 gcgaggttga agaccgggtt ggcggtcacg cggccggctc cacgaccgcg gacatcgaac
     1561 cgcgacagcg acggacgcgt ggatccggcc cgaatgcgtg aatctggcgc tggtggcggg
     1621 tcacctgttc gtgctgtgcc gtctccacgg tcgcgcggcc ctcggaatcc accatgcagg
     1681 cgagcgcaaa tgccttctcg gggtggtaac ccaggatgcg cccgagcagt tcgatcacgt
     1741 actgatatgt gtggtcatcg tcattcaaca ggacgaggtg gtagagatgt tcctcgtccg
     1801 gctcttggag ttgctcgcgc gtcacatcgg gcgtcgtcgt ctcgttcggc acgcgcgtat
     1861 tctgcgctct gaaagaagac gcggacaacg gaggcgggaa cattggacgt tgacgtcctc
     1921 gtcgtcggcg ccgggattgt cgggctggca ctagcggccg aactcgcctc cgactgctcc
     1981 gtcgtagtcg tcgagcggca cgcttcatac gcacgtgaaa cgagctcgca caacagcggc
     2041 gtgatccatt cggggatcta ctatcagacg ggctcgtgga agcaccgcct gtgcctcgag
     2101 gggaacgcag cgctctacga ctggtgcgcc cggcaaggcg taacgcatca gcgcctcggc
     2161 aaactgatcg tcgccacttc acgcgatgac ctcgaacagc tggaggcgat
//
LOCUS       DAORPG010000335         2209 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5964_length_2209_cov_3.051068, whole genome shotgun sequence.
ACCESSION   DAORPG010000335 DAORPG010000000
VERSION     DAORPG010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5964_length_2209_cov_3.051068"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="PLX85_07485"
     CDS             complement(<1..811)
                     /locus_tag="PLX85_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HRC63049.1"
                     /translation="MFERLAEIEARYEEVTRLLADPDVVSDHRRLQELGRERSQLETV
                     VALYRRYRDAQDALDDARALARDADEEMRTLGREEEDRLVAELAQLDQDLKLALLPRD
                     PADDKDVIVEIRGGTGGEEAALFAADLYRMYQRYAERQRWKTEVINTSEASAGGYKEI
                     VFEVHGQGAYSRLKYESGVHRVQRVPETEAQGRIHTSTATVAVLPEVDEVDVDIDWGQ
                     VRIDIFHSGGAGGQNVNKVATAVRMTHEPTGIVVTCQDERSQAKNRAKAEAV"
     gene            977..1405
                     /locus_tag="PLX85_07490"
     CDS             977..1405
                     /locus_tag="PLX85_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HRC63050.1"
                     /translation="MAEARVGELRDTRVDVKVVLGGLWTAMLFVFAYVDIFGFWREDV
                     INGALKGEVPGPGFEIGQTFLALTTVYILVPSLMVAVSLLAPARWNRRANVFVSLVYA
                     LTVVGSAVGETWAYYFLGSAVEVGLLLLIARTAWRWPRVA"
     gene            1436..>2209
                     /locus_tag="PLX85_07495"
     CDS             1436..>2209
                     /locus_tag="PLX85_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63051.1"
                     /translation="MVEVLTGNAASAALKDGRDDVPRSLLVRLGALLALGAAVAFLAA
                     CSDGGTAETANPFPERQRVLIVHSYDEGFRWTAEQGRGIVEGLGEAGLREGRDYELRT
                     FFMDTRITYTSPDAVSQQASEARRLIEEFEPSVVFVTDDAALREVAVRWVEEHPGSNV
                     AFVFSGVNVDPTAYKPIASLERPGGRITGLLERIPVAEASAAMQRVFPAATRVALLAD
                     GSASSAAAIADYTSKYPGGLQTPLRVTGFTQARTFAEWQR"
BASE COUNT          306 a          647 c          877 g          379 t
ORIGIN      
        1 ggacggcctc ggccttggcg cggttcttgg cctgtgagcg ctcgtcctgg caggtgacga
       61 cgatgccggt gggctcgtgg gtcatgcgca cggcggttgc gaccttgttg acgttctgac
      121 cgcccgcccc gcccgagtgg aagatgtcga tgcgcacctg accccagtcg atgtccacgt
      181 ccacttcgtc gacctccggg aggacggcga cggtggcggt cgaggtgtga atgcggccct
      241 gggcctcggt ctcggggacg cgctggacgc ggtgtacgcc ggactcgtac ttgaggcggg
      301 agtaggcgcc ctggccgtgc acctcgaaga cgatttcctt gtagccgcct gctgaggcct
      361 ccgaggtgtt gatcacctcg gtcttccagc gctggcgctc ggcgtagcgc tggtacatgc
      421 ggtagaggtc cgcggcgaag agggcggctt cctcgccgcc ggtgccgccg cggatctcga
      481 cgatcacgtc cttgtcgtcg gcggggtcgc gcgggagcaa tgcgagcttg aggtcctggt
      541 cgagctgggc gagttcggcg acgaggcggt cttcctcctc gcggccgagg gtgcgcatct
      601 cctcgtcggc gtcacgcgcg agggcccggg cgtcatcgag ggcgtcctgg gcgtcgcggt
      661 agcggcggta gagggcgacg acggtctcga gctgcgaacg ctcgcggcca agttcctgga
      721 ggcgacggtg gtcgctgacg acgtcggggt cggcgaggag gcgcgtgacc tcctcgtagc
      781 gggcttcgat ctcggccaag cgttcgaaca tgaagtcagg atagcagggc gggcggttac
      841 gccctcggga cgggagtggc gagtttgcca ccgcacgggg tgacgttcga atgggcgtcg
      901 tagagccata cgtccggtgc agatcgcggc agccgggtgc acgctacctg cgaaggtgcg
      961 aacggaggag gcaacgatgg ccgaagcgcg ggtcggtgag cttcgagaca cgcgagtgga
     1021 cgtgaaggtc gtcctcggcg ggctctggac cgccatgctg ttcgtcttcg cgtacgtcga
     1081 catcttcggg ttctggcggg aagacgtgat taacggcgcc ctgaagggcg aggtcccggg
     1141 accggggttc gagatcggtc agacgttcct tgcgctgacg acggtgtaca tcctcgtgcc
     1201 gagtctgatg gtggcagtat cgctgctggc tccggcgcga tggaaccggc gggcgaacgt
     1261 cttcgtgagt ctcgtgtacg ccctgaccgt ggtcgggtcg gcggtcggag agacatgggc
     1321 gtactacttc ctcggcagtg cggtggaggt agggctgctg ctgttgatcg cgcggacggc
     1381 gtggcggtgg ccgcgcgtgg cgtagcccgt gccggcgggc gcatcgcggc acgacgtggt
     1441 agaagtcctt accgggaacg cggcatcggc cgcgctgaaa gacgggcggg acgacgtgcc
     1501 tcgatcgctg ctggttcgcc tcggagcact gctggcactt ggcgccgcgg tcgcgttcct
     1561 cgccgcatgt tcggacggcg ggacggccga gacagcgaac ccgttcccgg agcggcagcg
     1621 ggtgctgatc gtgcattcgt acgacgaggg gttccggtgg accgcggaac aggggcgcgg
     1681 gatcgtcgag gggctgggcg aggcggggct gcgcgagggc cgcgactatg agttgcgaac
     1741 gttcttcatg gacacgcgga tcacgtacac gagccccgat gcggtctcgc agcaggcttc
     1801 cgaagcgcgg cggctgatcg aggagttcga accctcggtg gtgtttgtga cggacgacgc
     1861 cgcactgcgc gaggtggccg tgcggtgggt ggaggagcat cccggctcga acgtggcgtt
     1921 tgtgttctcg ggggtcaatg tcgacccgac ggcgtacaag ccgatcgcga gccttgagcg
     1981 gccgggcgga cgaatcacgg ggctgctcga gcggatcccg gtggcggaag cgtcggcggc
     2041 catgcaacgg gtgttccccg ccgcgacgcg ggtggcgctg ctggcggacg ggagcgcgag
     2101 ctcggcggcg gcgatcgccg actacacctc gaagtacccg ggagggctgc agacgccgct
     2161 gcgggtgacg gggttcacgc aggcgcggac gttcgcggag tggcagcgg
//
LOCUS       DAORPG010000336         2206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5980_length_2206_cov_2.463505, whole genome shotgun sequence.
ACCESSION   DAORPG010000336 DAORPG010000000
VERSION     DAORPG010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5980_length_2206_cov_2.463505"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1525)
                     /locus_tag="PLX85_07500"
     CDS             complement(<1..1525)
                     /locus_tag="PLX85_07500"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRC63052.1"
                     /translation="MTRWFGRTLREAPAEAETASHQLLLRAGAIDQLMAGVYTYLPLG
                     WRVKRKVEAIVREEMDAAGALEIHMPAIQPLDIWERSGRADAMGPVLFRLTDQRERPL
                     ALGPTHEEVVTQLFASQAQSYRDVPVTLYQIQTKFRDEARPRGGLVRVREFTMKDAYS
                     FDADEAGLDASYGAMFEAYKRIFARCGVPTVPVEADSGAIGGKESREFIYLSDTGEDT
                     IVICDRCGYAANTEKAEFVRPPVPPEEPLEVEPVETPGTTTIEALAAFLGVPASKTAK
                     AVFYMATDPHDASAAAEPVFVVVRGDLQVNEVKLRNLLGGKDLRPMTDAESASYGIVP
                     GYASPVGIDERVRVVVDTSIATSPNLVAGANVAGRHLRNVNLSREYHAHDVIDVALAE
                     AGHTCARCGEGTLGTARGIEMGHVFRLGYIYSEAFEARVLDEAGEQRTPIMGCYGLGV
                     DRVIAGAVQEHYDERGIIWPASIAPFDVHLVGLGLGRDAELASDADALYAELTAAGLE
                     "
     gene            1627..2007
                     /locus_tag="PLX85_07505"
     CDS             1627..2007
                     /locus_tag="PLX85_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63053.1"
                     /translation="MRRMAFSMLLAATVLACSRGGPSDRANDALLEELHAVPGAIEVG
                     RTQDRASTVVAYGIEDVPGTAVLEFYLASLPAEWELAAPLGDDPEAVALCRDGSLVRV
                     DAADVAARGTFTVTVRATGAEECD"
BASE COUNT          320 a          818 c          749 g          319 t
ORIGIN      
        1 cctcgagacc cgccgccgtc agctccgcgt agagcgcgtc ggcgtcggac gcaagctcgg
       61 cgtcgcgccc gaggcccagc ccgacgaggt gcacgtcgaa cggcgcgatc gaggcgggcc
      121 agatgatgcc gcgctcgtcg tagtgctcct gcaccgcgcc cgcgatcacg cggtccacgc
      181 ccaggccgta gcaacccatg atcggcgtgc gctgctcgcc cgcctcgtcc agcacgcgcg
      241 cctcgaaggc ctcggagtag atgtatccga ggcggaacac gtgccccatc tcgatgccgc
      301 gcgcggtgcc gagcgtgccc tcgccgcagc gcgcgcaggt gtgcccggcc tcggccagcg
      361 cgacgtcgat cacgtcgtgc gcgtggtatt cgcggctcag gttcacgttg cgcaggtgtc
      421 gccccgcgac gttggcgccc gccacgaggt tgggcgaggt cgcgatcgag gtgtcgacga
      481 ccacgcgcac gcgctcatcg atgccgaccg gcgaggcgta gccgggcacg atgccgtagg
      541 acgccgactc ggcgtcggtc atcggccgca ggtccttgcc gccgaggagg ttgcgcagct
      601 tcacctcgtt cacctgcagg tcgccgcgca cgacgacgaa cacgggctcc gccgccgccg
      661 aggcgtcatg cgggtcggtc gccatgtaga agacggcctt cgccgtcttc gaggcgggca
      721 cgccgaggaa cgctgccagc gcctcgatgg tcgtcgtgcc gggcgtctcg acgggctcca
      781 cctcgagcgg ctcctcgggc gggacgggcg ggcgcacgaa ttccgccttc tccgtgttcg
      841 ccgcgtagcc gcagcggtcg cagatgacga tggtgtcctc gccggtgtcg ctgaggtaga
      901 tgaactcgcg cgattccttg ccgccgatgg cgcccgagtc cgcctcgacg ggcacggtgg
      961 gtacgccgca gcgggcgaag atgcgcttgt atgcctcgaa catcgcgccg tacgacgcat
     1021 cgaggcccgc ctcgtccgcg tcgaacgagt acgcgtcctt catcgtgaac tcgcgcaccc
     1081 gcaccaggcc gccgcgcggg cgggcctcgt cgcggaactt cgtctggatc tggtacagcg
     1141 tgaccggcac gtcgcggtac gactgcgcct gcgaggcgaa gagctgcgtc acgacctcct
     1201 cgtgcgtcgg cccgagggcg agcggccgct cgcgctggtc ggtgagccgg aacagcaccg
     1261 gccccatcgc gtcagcgcgg ccggagcgct cccagatgtc gagcggctgg atcgccggca
     1321 tgtggatctc gagggcgccc gcggcgtcca tctcctcgcg cacgatcgcc tcgaccttgc
     1381 gcttcacccg ccagccgagc gggaggtacg tgtacacgcc cgccatcagc tggtcgatcg
     1441 cccccgcgcg cagcagcagc tgatggctgg ccgtctccgc ctccgcaggg gcctctcgca
     1501 acgtccgtcc gaaccagcgc gtcattctca tgcggctgag tctatgggcg cgtgcatggg
     1561 cgggcaacga gccgcgacgc ccaacgcgag gaggccccga accccatcag cgacgacaat
     1621 cgggccatgc gtaggatggc gttctcgatg ctcctggccg ccacggtcct cgcgtgctcc
     1681 agaggcggtc catccgatcg tgcaaacgac gcgctgctcg aagaactcca tgccgtgccc
     1741 ggtgcgatcg aggtgggacg cacgcaggac cgagcgagca ccgtcgtggc gtacgggatc
     1801 gaggacgtac ccggcacggc cgtcctcgag ttctacctcg cttcactgcc cgccgagtgg
     1861 gaactggccg cgccgctcgg cgacgacccg gaggccgtcg cactgtgccg cgacggcagt
     1921 ctcgtgcgag tcgacgccgc cgacgtggcc gcccgtggca cgttcaccgt taccgtccgc
     1981 gcaaccggcg ccgaggaatg cgactgaccg tccgcgccca cctcgcagcc gcgctaccct
     2041 agaccgcatg ccgtaccgag aagcccccac gcccggtcgc tgcatccatt gcaacagcga
     2101 gaccgcgatg gcgtgcgtcg cctgcgcgca ccacgtctgc agccgctgcc aggcccgaca
     2161 cgacgacgag ttcccccaca ggacctacac cggctgatcc gcggac
//
LOCUS       DAORPG010000337         2205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5985_length_2205_cov_3.404186, whole genome shotgun sequence.
ACCESSION   DAORPG010000337 DAORPG010000000
VERSION     DAORPG010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5985_length_2205_cov_3.404186"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="PLX85_07510"
     CDS             complement(<1..1261)
                     /locus_tag="PLX85_07510"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63054.1"
                     /translation="MLTRPPASSLRFTGAASFALGLLVAVGVLAAVFPWYPVGNHVAE
                     GDRSPWTLTAPRALSFDSAVRTEQVRAEAAAAVPDVFVLDPGVRDRELTLLDGQLSAI
                     RGIRDEPGLAASARESAIRAVRDVELSPRAAATLAAASAAEWDSMVTEAQAALGRALS
                     GTLQEKDLPGMRQQVAGFLSPLLSQDQRNALTELLSKHVVSTLVVDAARTNALRAEAR
                     QGTPPVHVSRARGDVIVSEGQQVTAADIELLEQAGLQVDGVRVRDVLAVALLAAIAGA
                     AVGGYLLVNQPASLASARRVTLLVLLLVLPAFGIKVALAVLLPDQDRHFFAYALPVAA
                     APMAAAVLLDVGLGVLLSVLLAIIAAFISVMLPLTHGVPAGEVETARLALAVSASSLA
                     GVYVAARAERLHRYLAAGLASGAAAAEI"
     gene            complement(1290..1742)
                     /locus_tag="PLX85_07515"
     CDS             complement(1290..1742)
                     /locus_tag="PLX85_07515"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRC63055.1"
                     /translation="MPLVQTLRDDMTAAWRAGDVDRRNTLRLLIAALDYARIAAGHDL
                     TDPEAIAALQREARQRRDSIEQYRAGGRDDLVAHEQSELDLITSYLPADLTDEELADL
                     VRAAIAEAGATGPSDLGKVMGPLMKRVAGRAEGGRVNALVREMLASPA"
     gene            complement(1790..1996)
                     /gene="rpsU"
                     /locus_tag="PLX85_07520"
     CDS             complement(1790..1996)
                     /gene="rpsU"
                     /locus_tag="PLX85_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRC63056.1"
                     /translation="MSEVLVGDMESLESALKRFNKRVQADGILSDARRHEYYEKPSVR
                     RKKKEAARIRKLRKAIRTQAARQR"
BASE COUNT          350 a          689 c          871 g          295 t
ORIGIN      
        1 cgatctcggc ggcggcggcg cccgaggcga gcccggccgc gaggtagcga tgcaggcgct
       61 cggcgcgcgc cgcgacgtag acgccggcaa gtgacgacgc ggagacagcg agggcgagcc
      121 tcgcggtctc gacctcgccg gccgggacgc catgtgtgag cgggagcatg acggagatga
      181 aggccgcgat gatggcgagc agcaccgaga gcagtacgcc gagaccgaca tcgagcagga
      241 cggctgcggc catgggagcg gcggccaccg gcaaggcgta ggcgaagaag tggcggtcct
      301 gatcggggag gagcacggcg agggcgacct tgatgccgaa cgccggcagc acgaggagca
      361 ggaccagcag cgtgacgcgc cgggcgcttg cgagcgacgc gggctgattg acgagcaggt
      421 agccgcccac cgccgcgccg gcgatggcgg ccagcagggc aaccgccagc acgtcgcgga
      481 cgcggacgcc atccacctgg agtcccgcct gctcaagcag ttcgatgtcg gcagcggtga
      541 cctgctggcc ctcggagacg atcacgtcgc cgcgggcgcg gctcacatgg acgggcgggg
      601 tgccctggcg ggcttcggca cggagggcgt tggtgcgggc ggcgtctacg acgagcgtgg
      661 agacgacgtg cttcgagagc agttcggtga gcgcgttgcg ctggtcctgc gacagcagcg
      721 gcgagaggaa gccggcgacc tgctggcgca tgcccgggag gtccttctcc tggagtgtgc
      781 cggagagggc gcggccgagg gcagcctgcg cttcggtgac catggagtcc cactcggccg
      841 cactcgcagc ggcgagcgtg gcggcggcgc gcggcgatag ttcgacgtcc ctcacggcgc
      901 ggatggcgct ctcgcgcgcg gaggcagcga ggccaggctc atctcgaatg ccacggatcg
      961 cggaaagctg gccatcgagg agggtcaact cgcggtcgcg cacccccgga tcgagtacga
     1021 agacgtcggg gaccgccgcc gcggcctcgg cgcgcacctg ctcggtgcga acggcggagt
     1081 cgaaggagag ggcgcgcggc gccgtcaggg tccagggaga gcggtcgccc tcggcgacgt
     1141 ggttgcctac gggataccac gggaagacgg ccgcgaggac gccaacggcg accaggaggc
     1201 cgagcgcgaa gctggcggct ccagtaaagc gcagtgagga cgccgggggg cgagtcagca
     1261 tggcagggtg cctcgagggc gccggggggt catgcgggag aggctagcat ctcccgaacg
     1321 agggcgttga cgcggccgcc ctctgcccgt ccggccacgc gtttcatgag cggccccatg
     1381 accttgccga ggtcggaagg accggtcgcg ccggcctcgg cgatggcggc acgcacgaga
     1441 tccgccagct cctcatcggt gaggtcggcg gggaggtacg aggtgatgag gtcgagctcg
     1501 gactgctcgt gagcgacgag gtcatcgcgg ccgccggcgc ggtactgctc gatggagtcg
     1561 cggcgctggc gggcctcgcg ctggagggcg gctatggcct cggggtcggt gaggtcgtgg
     1621 ccggccgcga tacgggcgta gtcgagagcg gcgataagca gccggagcgt gttgcgtcgg
     1681 tcgacgtcac cggcgcgcca ggcggcggtc atgtcatcgc ggagggtctg gacgagtggc
     1741 atgggagcac cggagatgca gagccccggc agagccgggg tctacgaggt tagcgctggc
     1801 gggcggcctg cgttcggatc gccttgcgca acttgcggat gcgagccgct tccttcttct
     1861 tgcgtcggac ggacggcttc tcgtagtact cgtgccgccg ggcgtccgag aggatgccat
     1921 ccgcctgaac gcgcttgttg aagcgcttca gcgcggactc gagggattcc atgtctccaa
     1981 ccaggacttc gctcactgcg tctccttcac ctcccttccc gaccggacat acgtgtcgcg
     2041 ccgggcgcga ccgtccctcg atggtaggag tgggcttccg acggcgtcaa atgaaacggt
     2101 gcgggagatc agcccaagaa cgcacaggag acggcgagcg tctcctgtgt gtccatgcga
     2161 tcccgcgatc cgcgatcagt cggcggaggg caacaccttc ggctg
//
LOCUS       DAORPG010000338         2202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_5999_length_2202_cov_4.281323, whole genome shotgun sequence.
ACCESSION   DAORPG010000338 DAORPG010000000
VERSION     DAORPG010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5999_length_2202_cov_4.281323"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /gene="pgi"
                     /locus_tag="PLX85_07525"
     CDS             <1..864
                     /gene="pgi"
                     /locus_tag="PLX85_07525"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449176.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRC63057.1"
                     /translation="DPANMFGFWDWVGGRYSMDSAIGLSLMLAIGARQFRELLEGFRS
                     IDEHVRTAPLERNIPVLMALLTIWYTGCFGVETHGVMPYAQYLASFPAYLQQLTMESN
                     GKRVTAEGKAIPYPTGPVVWGEPGTNSQHSFFQLLHQGTRLVPVDFIGFRRAVEDVGE
                     HQDLLTANLLAQAAALAFGRTPEELRDRGVPERLVPHRASEGNRPSSVLLLDRLTPAA
                     LGQLIALYEHRVYVEGVLWDIDSFDQWGVELGKEIAGQILPALRGETSTTAYDSSTAA
                     LIERYRTARER"
     gene            944..1321
                     /locus_tag="PLX85_07530"
     CDS             944..1321
                     /locus_tag="PLX85_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63058.1"
                     /translation="MTEQLTADGVNGLVELDGDTVRIWVKGALSLVNPDMMRCKEIYL
                     GDIASIQYRDAGLLTHGYIQFTHFGGGPRARVDEAVRDGSAVMFRRRHQADFDAIYDA
                     IEAARRDLRSRMVRSNDAPPPEG"
     gene            complement(1347..>2202)
                     /locus_tag="PLX85_07535"
     CDS             complement(1347..>2202)
                     /locus_tag="PLX85_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRC63059.1"
                     /translation="MAVGGVLIAAYVRHARVAPAPILDLKLLRIETYRAGVLGGSLFR
                     IGVGAIPFLLPLMLLSFGWVGANSAQASWFGALVSDGPRDQPYVALTFDDGPNGDDTL
                     RLAAILAAHDARGTFFVVGRTVEEQPEVVRTLIEDGDLVGNHSYEHTRWGYLDPRYPE
                     LDRTEAAIERVTGRCPTYYRPPHGAHTPFISRLLRDRGMHMVTWEVQANDWKETDAAR
                     LARSILDQVHPGAIVLLHDGIDGLPGADRSVLIDALPLILDGLRERGLQPVTLDRLLG
                     GPGYLDHC"
BASE COUNT          356 a          810 c          695 g          341 t
ORIGIN      
        1 gacccggcga acatgttcgg cttctgggac tgggtcggcg gtcgctactc gatggactcc
       61 gccatcggcc tctccctcat gctcgccatc ggcgcccggc agtttcgcga actcctcgag
      121 ggcttccgct ccatcgatga gcacgtgcgg acggcgccgc tcgagcgcaa catccccgtg
      181 ctcatggcgc tgctcacgat ctggtacacc ggctgcttcg gcgtcgagac ccacggcgtg
      241 atgccgtacg cgcagtatct cgcttcgttc cccgcctatc tccaacaact cacgatggag
      301 agcaacggca agcgcgtgac cgccgagggc aaagcgatcc cttaccccac cggccccgtc
      361 gtctggggtg agccgggcac gaacagccag cactcgttct tccagctgtt gcaccagggc
      421 acacgcctcg tcccggtcga cttcatcggc ttccgccggg ccgtcgagga cgtcggcgag
      481 caccaggacc tgttgaccgc caacctgctc gctcaggccg ccgccctcgc cttcggccgt
      541 actcccgagg agttgcgcga ccgcggcgtc cctgagcgcc tcgtccccca ccgggcgagc
      601 gagggcaacc gcccctcgag cgttctcctc ctcgaccgtc tcacgcccgc ggccctcggc
      661 cagctcatcg ccctgtacga gcacagggtc tacgtcgagg gcgtgctgtg ggacatcgat
      721 tcgttcgacc agtggggggt tgagctgggc aaggaaatcg ccggacagat cctccccgcc
      781 cttcggggtg agacctcaac gaccgcctac gacagctcga ccgccgcgct catcgagcgg
      841 taccgcaccg cccgcgagcg ctgagcccga ggtcaccgcg gccctcgacc ggggtgctac
      901 catgcgcccg tccgcgaggt ggccggcacc tgaggcgcca accatgacgg aacagctcac
      961 cgccgacggc gtgaacgggc tcgtggagct cgatggcgac accgtgcgca tctgggtcaa
     1021 gggcgcgctc tccctcgtca atcccgacat gatgcgctgt aaagagatct acctcggcga
     1081 catcgcgtcc attcagtacc gcgacgccgg cctcctcacc cacggctaca tccagttcac
     1141 acacttcggc ggcggaccgc gcgcccgcgt cgacgaagcc gtccgcgacg gcagcgccgt
     1201 gatgttccgc cgccgccatc aggccgactt cgatgccatc tacgacgcca tcgaggcggc
     1261 ccgtcgcgac ctgcgttcga gaatggtccg ctcgaacgac gctccgccgc ccgagggctg
     1321 agcgagcccg gccgccgcat cactcgtcag cagtgatcga ggtatcccgg gccgcccagg
     1381 agccggtcga gcgtgaccgg ttgcagtcct cgctcccgca gtccgtcgag aatcaacggc
     1441 agcgcgtcga tcagcaccga ccggtccgca ccgggcagcc cgtcgatccc gtcatgcagc
     1501 agcacgatgg cccccggatg cacctggtcg aggatcgagc gcgccagccg cgccgcatcg
     1561 gtttctttcc agtcgttcgc ctgcacctcc cacgtcacca tgtgcatgcc tcggtcgcgc
     1621 aggagccttg atatgaatgg cgtgtgagcg ccgtggggag gccggtagta ggtgggacaa
     1681 cgcccggtca cccgctcgat ggcggcttca gtgcggtcga gctccgggta ccgcgggtcg
     1741 aggtagcccc accgcgtgtg ctcgtatgag tggttgccga ccagatcgcc gtcctcgatc
     1801 agcgtacgca cgacctccgg ttgctcctcg accgtgcgac cgacgacgaa gaaggtgccc
     1861 ctggcatcgt gtgccgcgag gattgccgcg agtcgcagcg tgtcgtcacc gttcgggccg
     1921 tcgtcgaagg tcaacgcgac gtacggctgg tcgcggggtc cgtcgctcac cagcgctccg
     1981 aaccacgagg cctgcgccga gttcgcccca acccagccga acgagagcag catcaacggc
     2041 agcaggaacg gaatggcgcc gacaccgatg cggaacaggc tgccgccgag cactccggcg
     2101 cgataagtct cgatgcgcag cagcttgaga tcaaggatcg gcgcgggcgc gacgcgggcg
     2161 tggcgcacat aggcggcgat caacacgccg cccaccgcca tc
//
LOCUS       DAORPG010000339         2197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6031_length_2197_cov_3.647993, whole genome shotgun sequence.
ACCESSION   DAORPG010000339 DAORPG010000000
VERSION     DAORPG010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6031_length_2197_cov_3.647993"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLX85_07540"
     CDS             <1..283
                     /locus_tag="PLX85_07540"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HRC63060.1"
                     /translation="TLAGSREDDPLGGEMRRMHTDVLDRIESFVRQIQDAGELRRDID
                     PHALALTILSAVQGLRMLFVEFDEQIETEGVLEILNRMLRGFMPVREEN"
     gene            286..>2197
                     /locus_tag="PLX85_07545"
     CDS             286..>2197
                     /locus_tag="PLX85_07545"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRC63061.1"
                     /translation="MASPLSTGGLARAAARKPWLTIAIWAVILVAGMGIAGAGLSGVL
                     TQEQKITSKPESVQAEDALEASGIPGRDQMPAIEAVILRSDSLKATDAEFKTFADSLL
                     KDIRALPDVANATSYFETNAPTMVSPDQHKVLLPVTLVGGVPNVEDHIDPLLQLIDER
                     NGSDKFDVLTFGMGSVNKAFSTTAEHDLQTAEQRGMPVALIVLVLVFGAVVAAVIPIV
                     LAVAGIMVALGVTTLVGQIWSLSFFVTNMIFMIGLAVGIDYSLFIIERFREERRHGLD
                     KIEAIATAGNTASRAVVFSGFMVVIALVGMLLVPSNIFRALGAGAIIVVLVALAVTTT
                     LLPAVLSLLGDNVNRLGIPFFSRKGQVDESRGFWAGAARLVMGHPWISAISATAFLVA
                     LSIPYFSISLGLSGADTLPRSTQVWEANDILAREFSQGLAQPTEIVVTAPDVNAPAVK
                     SGLDKLQAAMAADTKGPDGKPRFSNAQVTVGPNKDVALLSVAVAGDPASEESLGAIKD
                     LRSSMAPAAFGGSGAKVYVGGISAGNQDFFDVVDKYEPIVFGFVLALSFVLLTMVFRS
                     IVVPLKAVVMNLLSVGATYGLLVLVFQKGVGADLLHFQQSPIIQAWIPLFLFSILFGL
                     SMDYHVFLLSRIR"
BASE COUNT          341 a          670 c          773 g          413 t
ORIGIN      
        1 gacgctggcg gggagccgcg aggacgaccc gcttggcggc gagatgcggc gcatgcacac
       61 ggacgtgctc gatcggatcg agtcgttcgt gcggcagatc caggatgcgg gtgagctgcg
      121 acgtgacatc gacccgcacg cactcgcgct gaccattctg tcggccgtgc agggcctgcg
      181 gatgttgttc gtcgagttcg acgagcagat cgagacggag ggcgtattgg agattctcaa
      241 ccggatgctg cgcggcttca tgcctgttcg ggaggagaac tagggatggc atcgccgttg
      301 tctaccggtg ggctggcgcg ggcggccgct cgcaagccat ggctcaccat tgcgatctgg
      361 gccgtcatcc tcgtcgccgg catgggtatc gccggcgccg ggttgagcgg cgtgctgaca
      421 caggaacaga agatcacgag caagccggag tcggtacagg ccgaggacgc gctcgaggcg
      481 agcggcatcc ctggccgtga ccagatgccg gccatcgagg ccgtgatcct gcgctcggat
      541 tcgttgaagg cgaccgacgc cgagttcaag acgttcgccg actcactgct caaggacatc
      601 cgtgcgctgc cggatgtcgc gaatgcaacg agctacttcg agacgaatgc tcccacgatg
      661 gtgtcgcccg accagcacaa ggtgctgctg ccggtgacgc tcgtgggcgg cgtcccaaat
      721 gttgaggacc acatcgatcc gctgctacag ctcatcgatg agcggaacgg cagcgacaag
      781 ttcgacgtgc tgaccttcgg catgggaagc gtcaacaagg cgttctcgac aacggccgag
      841 cacgacctgc agaccgcgga acagcggggc atgccggtgg cgctgatcgt cctcgtgctg
      901 gtgttcggcg cggtggtggc ggcagtcatc ccgatcgtgc tcgcggtggc cggaatcatg
      961 gtggccctgg gcgtgacgac gctggtcggt cagatctggt cgctctcgtt cttcgtcacg
     1021 aacatgatct tcatgatcgg cctcgcggtg ggcatcgatt actcgctgtt catcatcgag
     1081 cgcttccggg aagagcgccg tcacgggctc gacaagatcg aggcgatcgc aacggcgggg
     1141 aacacggcca gccgggccgt ggtgttctcc gggttcatgg tcgtgatcgc actggtcggc
     1201 atgctgctcg tgccctcgaa catcttccgg gcgctgggtg caggggcgat catcgtcgtg
     1261 ctcgtggcgc tggcggtgac gacgacgttg ctgccggcgg tgctcagcct gctcggcgac
     1321 aacgtaaacc gcctcggcat cccgtttttc tcgcgcaagg gacaggtcga tgagtcgcgc
     1381 ggcttctggg ccggtgcggc gcggctggtg atggggcacc cgtggatcag cgccatctcg
     1441 gcgaccgcgt tcctcgttgc cctgtcgatt ccgtacttct cgatcagcct cggcctctcg
     1501 ggcgcggaca ccctgccgcg gtcgacgcag gtgtgggagg cgaacgacat cctggcgcgt
     1561 gaattctcac agggcctcgc gcagccgacc gagattgtcg tgacggcgcc ggacgtaaac
     1621 gcgccggcgg tgaagtccgg cctcgacaag ctgcaggcgg cgatggccgc ggacaccaag
     1681 gggcccgacg gcaagcctcg cttcagcaac gcgcaggtga cggtcggtcc gaacaaggac
     1741 gtggccctgc tgtcggtggc cgtcgccggc gacccggcca gtgaggagtc cctgggggcg
     1801 atcaaggatc tgcgctcctc gatggcgcct gccgcgttcg ggggctccgg ggcgaaggtc
     1861 tacgtgggag gcatctccgc gggcaaccag gacttcttcg atgtggtcga caagtacgag
     1921 ccaattgtgt tcgggttcgt gctcgcgctg agcttcgtgc tgctgacgat ggtcttccgg
     1981 tcgatcgtcg tgccgctgaa ggccgtcgtg atgaacctgc tctcggtggg cgcgacctac
     2041 ggcctgctgg tgctggtgtt ccagaagggc gtgggcgcgg acctgctgca cttccagcag
     2101 tcgcccatca ttcaggcctg gatcccgttg ttcctgttct cgatcctgtt cgggctctcg
     2161 atggactacc acgtgttcct gctgagccgg atccggg
//
LOCUS       DAORPG010000340         2196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6034_length_2196_cov_3.019617, whole genome shotgun sequence.
ACCESSION   DAORPG010000340 DAORPG010000000
VERSION     DAORPG010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6034_length_2196_cov_3.019617"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PLX85_07550"
     CDS             <1..356
                     /locus_tag="PLX85_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63062.1"
                     /translation="AVVAAPHLGPLVEALQHEGVPEDVLTVARGDVVARAGEAWVDLV
                     VTDSGASTCRAAYQALGPTAEGRTALKSLLSPLDGAQPGEPGFVQRFAWPRVIATRTL
                     RHGADLALESRARGE"
     gene            420..496
                     /locus_tag="PLX85_07555"
     tRNA            420..496
                     /locus_tag="PLX85_07555"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:454..456,aa:Val,seq:cac)
     gene            524..610
                     /locus_tag="PLX85_07560"
     tRNA            524..610
                     /locus_tag="PLX85_07560"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:558..560,aa:Leu,seq:cag)
     gene            633..708
                     /locus_tag="PLX85_07565"
     tRNA            633..708
                     /locus_tag="PLX85_07565"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:666..668,aa:Arg,seq:gcg)
     gene            782..>2196
                     /gene="uvrC"
                     /locus_tag="PLX85_07570"
     CDS             782..>2196
                     /gene="uvrC"
                     /locus_tag="PLX85_07570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRC63063.1"
                     /translation="MTDPGVLQQVRALPERPGVYIMRDVRGDVLYVGKATNLRSRVRS
                     YFGSSRSLEPKVLALAQLVVTVDHVLTRNEAEALLLEATLVKRHQPQYNVRLKDDKHY
                     PYLKVDVQNPWPRVTITRRVEADGARYFGPYASAGSVRRTLDVVKKLFPWRSCTKPIT
                     GTDPRPCLDYYIKRCIAPCTAFCTKEEYDEVINQTVLFLDGRSREVLDHVEAEMLKAS
                     DDLEFERAARLRDQAEAIRRVTERQQMATTTPLDADVFALARSGDEACVQVFFVRGTA
                     VADTDSFMLDGTQDASDAEVLSAFLAQFFESATYVPRNVLLSMRPDDLEELQAALRER
                     RGSAVEIRVPERGELRQLVASAEENARSTLAAHRVRWMSDRDKTQAAMTLLQDELDLP
                     APPNRIECYDISTIQGTNTVASMVVFEQGAPVNGQYRRFRIKTVEGQDDFASMREVLT
                     RRFSRLAASRRAISSPSARESP"
BASE COUNT          362 a          724 c          776 g          334 t
ORIGIN      
        1 tcgcagtcgt cgcggctccg cacctcggcc cgctcgtcga ggcgctgcag cacgagggcg
       61 tgcccgagga cgtgctcacc gtggcgcgag gcgacgtggt ggcacgggcg ggcgaggcgt
      121 gggtcgacct cgtggtgacc gacagcggag cctcgacgtg ccgggctgcg taccaggcgc
      181 tggggccgac cgcggagggc cgcaccgcgc tgaagtccct actgtcaccc ctcgacggag
      241 cccagccggg cgagccgggg ttcgtgcagc ggttcgcctg gcctcgcgtc atcgcgacgc
      301 ggacgctccg gcacggcgcg gacctcgcgc tcgaatcacg tgcccgtggt gagtgaggcg
      361 accgagttga accgggtgag cgcgggcgat acgctaggca ttcgcgaagg cgtcgacgcg
      421 ggccgttagc tcagttggta cgagcgctcg cttcacacgc gagaggtcag tggttcaagt
      481 ccactacggc ccaccagcga aaggacgcag acgcgaagtt cgggccgaag tggcggaata
      541 ggcagacgcg ctacgttcag ggcgtagtga gggcaacctc gtgcgagttc aagtctcgcc
      601 ttcggcacca ggtttgacgt tctgtttgac gcgcgctcgt agctcagggg atagagcgca
      661 gccctgcgga ggctgaggtc gtaggttcaa atcctaccgg gcgcgccact tcaaccatct
      721 cgttcgaccg tcccgaccgc cctcgaatcg gcgccccggc ggcggcgcta cacttgaact
      781 cgtgaccgat cccggcgtcc tgcagcaggt acgagcgctt cccgagcgcc caggcgtcta
      841 catcatgcga gacgtccgcg gggacgtgct gtacgtcggc aaggcgacga acctccggtc
      901 gcgggtgcgg tcctacttcg gatcatcgcg gagcctcgag ccgaaggtgc tggcactggc
      961 gcagctcgtg gtcaccgttg accacgtgct gacgcgaaac gaggcggagg ccctgctcct
     1021 cgaggcaacg ctcgtgaagc ggcaccagcc gcagtacaac gtgcggctga aggacgacaa
     1081 gcactacccg tacctgaagg tggatgtgca aaacccgtgg ccgcgggtga cgatcacgcg
     1141 gcgcgtcgag gccgacgggg cgcgctactt cgggccatac gcgtctgcgg gatcggtgcg
     1201 gcggacgctc gacgtggtga agaagctgtt tccctggcgg tcatgcacga agccgatcac
     1261 cggcaccgac ccccgtccct gcctcgacta ctacatcaag cgctgcatcg ccccatgcac
     1321 cgccttctgt acgaaggagg agtacgacga ggtcatcaac cagaccgtgc tcttcctcga
     1381 cggacggagc cgcgaggtac tcgaccacgt cgaggcggag atgctcaagg cttcggacga
     1441 cctcgagttc gagcgcgcgg cacgcctgcg cgaccaggcg gaggcgatcc ggcgagtgac
     1501 cgagcgccag cagatggcga cgacgacgcc gctcgacgcg gacgtgttcg cgctcgcgcg
     1561 gtccggtgac gaggcgtgcg tgcaggtgtt cttcgtgcgc ggcacggcgg tcgcggacac
     1621 cgattccttc atgctcgacg ggacccagga tgcctcggac gccgaggtgc tgtcagcgtt
     1681 cctcgcgcag ttcttcgagt cggctacgta tgtgcctcgc aacgtcctgc tgtcgatgcg
     1741 accggacgat ctcgaggagc tgcaggcggc gctgcgcgag cggcgcggga gcgccgtcga
     1801 gatccgcgtg ccggagcgag gggagctgcg ccagctggtc gcgagcgccg aggagaacgc
     1861 ccgctcgacg ctcgccgctc accgcgtgcg ctggatgtcg gaccgcgaca agacgcaggc
     1921 tgcgatgacg ctgctgcagg acgagctcga tctcccggca ccgccgaacc ggatcgagtg
     1981 ctacgacatc tcgacgatcc aggggacgaa cactgtcgcc tcgatggtgg tcttcgagca
     2041 gggcgccccg gtgaacggcc agtaccggcg cttccgcatc aagaccgtcg aggggcagga
     2101 cgacttcgcc tcgatgcggg aggtgctgac gaggcgcttc tcgcggcttg ccgcaagccg
     2161 ccgggcgatc tccagtcctt ccgcgcgcga aagccc
//
LOCUS       DAORPG010000341         2189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6070_length_2189_cov_3.379100, whole genome shotgun sequence.
ACCESSION   DAORPG010000341 DAORPG010000000
VERSION     DAORPG010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6070_length_2189_cov_3.379100"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(288..989)
                     /locus_tag="PLX85_07575"
     CDS             complement(288..989)
                     /locus_tag="PLX85_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63064.1"
                     /translation="MNHYLVIAHRTAARPELTECLTRLAAADPRAAITLLVTASHGPL
                     EEHTAGVTSRTIALADRARADLSAAGVHPVRTLVGVGSPLVAAQDELIERPDTYHAIV
                     LLTRPPRLLGRLAGNLRARLEDESGLPVFHAYRGWLEPWREGRQRPPGRLARIWRRTR
                     FEQRRDPTEPALRPEPPKGRDLLPVAALMLCYLAGGLTLALTVNRGFYLNDAVAIVVY
                     TVVIGGLLLVMRREM"
     gene            1049..1450
                     /locus_tag="PLX85_07580"
     CDS             1049..1450
                     /locus_tag="PLX85_07580"
                     /inference="COORDINATES: protein motif:HMM:NF017935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HRC63065.1"
                     /translation="MRPGVSFLDALHVLWLGALGRCPGCRTQPMFRGFFTLYERCPNC
                     GIRYEREDGAWLGATALGYSVGALVAVFLGLIELRWHPIGALGLHPLWTIMVVSLLVT
                     VPAYRPAKGVWFALLWLYEFTDEAEPSADAR"
     gene            complement(1467..1790)
                     /locus_tag="PLX85_07585"
     CDS             complement(1467..1790)
                     /locus_tag="PLX85_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HRC63066.1"
                     /translation="MIYELRTYTAMPGKLPALNARFRDHTVKLFERHGLRCIGFWTYK
                     HGGSSDQLVYLMAFEDQAARDAAWAAFQADTEWQQVRAASEVEGPLVAHITSDILLPT
                     DYSPA"
     gene            1901..>2189
                     /locus_tag="PLX85_07590"
     CDS             1901..>2189
                     /locus_tag="PLX85_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63067.1"
                     /translation="MQLNEMAEGLEPLDLTGTAEFASLLQSATDLGIPHSPGVRYVSR
                     QTVVGGHRFHFLEWGERSAPAVLLLHGGNQTAHAWDLVSLHLSDRYHVYALD"
BASE COUNT          289 a          643 c          914 g          343 t
ORIGIN      
        1 ctacggcggt ggtggtggcg gcggttacgg tgggggtggc tacggccgcc gctaaccgca
       61 ggtggcaggc ggcgctcggg tggcgccgct gacctcggaa cggtgcttgt gaacaggccc
      121 ggcgatacgc cgggcctgtt gctttgcgcg ggggacgatg gtcctcggaa gcggccgctg
      181 cgggtggcgc cgagtggtgt gaggggaagt tttgggttcc tcggagcgcg gtcggccgct
      241 tcgtcggggg tcgctcctca acgggtgagt ccgactcgcg ccggccgcta catctcgcgg
      301 cgcataacga ggaggaggcc gccgatcacg acggtgtaga cgacgatcgc gacggcgtcg
      361 ttcaggtaga agccgcggtt cacggtgagg gcgagcgtga ggccgcccgc gaggtagcag
      421 agcatgagag cggcgacggg gagcaggtcg cggccctttg ggggttcggg gcgaagggcg
      481 ggctcagtgg ggtcgcggcg ctgctcgaag cgggtacgac gccagatccg cgcgaggcgg
      541 ccgggcggcc gctggcggcc ctcgcgccag ggttcgagcc agccgcgata ggcgtggaag
      601 acgggcaggc ctgactcgtc ctcgaggcgg gcgcgcaggt tgccggcgag acggccaagg
      661 agcctcggcg ggcgggtgag cagcacgatg gcgtggtagg tgtcggggcg ctcgatgagc
      721 tcgtcctggg cggcgaccag gggggagccg acgccgacga gggtgcgcac gggatggacg
      781 cccgccgcgg agaggtccgc ccgcgcacgg tcggcgagag cgatggtgcg gctggtcacg
      841 ccggccgtgt gttcctcgag ggggccgtgg gaggccgtga cgaggagggt gatggccgcc
      901 cgcgggtccg cggccgcgag gcgcgtgagg cactcagtca gctcgggtct ggctgccgtg
      961 cggtgtgcga tgacgaggta gtggttcacc cgtgaccccg cgtggtgaga gagcggactt
     1021 cgatgatagc ggcctgtacg atcggggtat gcgaccgggt gtgagcttcc tcgatgcgct
     1081 gcatgtgctg tggctgggtg cgctcgggcg atgccccggg tgccggacgc agccgatgtt
     1141 ccgcggcttc ttcacgctct acgagcggtg ccccaactgc ggcatccggt acgagcgtga
     1201 ggacggggcc tggctcgggg cgacggcgct ggggtactcg gtgggcgcgc tggtggcggt
     1261 gttcctcggc ctgatcgagt tgcgctggca tcccatcggc gcgctggggc tgcacccgct
     1321 gtggacgatc atggtggtga gcctgctggt gacggtgccg gcgtaccggc cggcgaaggg
     1381 cgtgtggttc gcgctgctgt ggctgtacga gttcaccgat gaggcggagc cctcggcgga
     1441 cgcgcgatga gtcctcggct gccgcgctag gcgggcgagt agtcggtggg cagcaggatg
     1501 tccgaggtga tgtgggcgac gagcgggcct tcgacctcgg aggcggcgcg gacctgttgc
     1561 cactcggtgt cggcctggaa ggcggcccag gcggcgtcgc gggcggcctg atcctcgaag
     1621 gccatcaggt agacgagctg gtcgctcgaa ccgccgtgct tgtaggtcca gaagccgatg
     1681 cagcgcaggc catggcgctc gaagagcttc acggtgtggt cgcggaagcg ggcgttgagg
     1741 gcggggagct tgccgggcat ggccgtatac gtccgcagct catagatcat cgtgtgctcc
     1801 tccgtaacgg ctcagggcgg cgccgtgcgc cctcgatagg cgctgttata gtgcgcggcg
     1861 accgccgaga caacgcgggc ggagcggcga ggagcgtggc gtgcagctga acgaaatggc
     1921 ggagggcctc gagccgctgg atctgaccgg gacggcggag ttcgcgagcc tgctgcagag
     1981 cgcgaccgac ctcgggatcc cgcactcgcc gggggtgcgg tacgtgtcga ggcagaccgt
     2041 tgtgggcggt caccggttcc acttcctcga gtggggggag cgctccgcgc ccgcggtgct
     2101 gctgctgcac ggcgggaacc agaccgccca tgcttgggac ctcgtcagcc tgcacctgtc
     2161 ggaccgctac cacgtgtacg cactcgacc
//
LOCUS       DAORPG010000342         2187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6082_length_2187_cov_3.272514, whole genome shotgun sequence.
ACCESSION   DAORPG010000342 DAORPG010000000
VERSION     DAORPG010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6082_length_2187_cov_3.272514"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2187
                     /locus_tag="PLX85_07595"
     CDS             <1..>2187
                     /locus_tag="PLX85_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63068.1"
                     /translation="GLPLPAIEYELTVLNVDRPGEPARWPALESQGMAWVPGAKPGEA
                     ARVVWVQAREGRDWVVAGDPTTGAVTALFEVELPVSLYGAPDGRAFVVIDRLYDAPKR
                     IRFYDAERSKLIDVAASRLDPGPVSWAPDSSVVLIAGDRTLMLASDGGPIRLDVELPF
                     ERVAWSPDSTWAIAALRELPTENAAPQNELVRIGVGTGTATHLLDEVAQGRVLTSPGA
                     AVSPDGRRFALAWWDPSEPASKISVFDRDGPLPLRLEDRVVASYPAPGAPGTLQSLTS
                     MSWTPDGTSLVVSMFAPLNGQMYPPDTSIQVIDIASKSVRLVVAPRDGHYAYGPMWVS
                     SDGRALFKQWSSCVGCDGGRSGIDVIDLADGAIVRTIEDSVALGTVDGVRYLLSSPEG
                     LLSVRGLEPPRVLMPAKWRGSTWFSVRPSPDGNRLAVAQAAGRTSPVIAVAPDGSAQT
                     VLGYVRPEASVVGLRDASTAFVLDYEAGWAWSSFADGAVTPLPQQGEARPEAALETAV
                     AVSPDRSVAAYWVPGPSKPEVTLQLLELRTGAVTSSVATSSQMKGMYLAMSVDGKRVL
                     FPDGDVLVAVDVLTRSTVRIPLRRLGHPAGIGIVSSATYGPGGTFEVVAGDKLWRIDP
                     ASGDARVVTDRQPSPGGWRSSVQLSRSPDGQRLVALTAFGLFELQLDGSWRLASGVGL
                     DSRGPVATSIVWSPDSRRVAYLGSEGPGVPGALGIVVVGLDGSGAY"
BASE COUNT          288 a          683 c          854 g          362 t
ORIGIN      
        1 ccgggctgcc gctgccggcg atcgagtatg agctgaccgt gctgaacgtc gaccggccgg
       61 gggagccggc gcgctggccg gcgctcgaga gccagggcat ggcgtgggtc cccggggcga
      121 agcccggcga ggcggcgcgc gtggtgtggg tacaggcgcg tgagggccgg gactgggtgg
      181 tcgccggcga ccccacgact ggcgcggtca ctgcgctgtt cgaggtggag ctgccggtct
      241 cgctctacgg tgcgccggac ggccgggcat tcgtcgtcat cgaccgcctg tatgacgcgc
      301 cgaagcggat ccgcttttac gacgcggaac gttcgaagtt gatcgatgtc gcggcgagtc
      361 gcctcgaccc agggccagtg agctgggcgc cggactcgag cgtggtactc atcgcgggcg
      421 atcgaacgtt gatgctcgcg agcgacggtg gcccgatccg cctcgacgtg gaattgccgt
      481 ttgaacgagt ggcgtggtcg ccggactcga cgtgggcgat cgcggcgctc agggaactcc
      541 cgacggagaa cgccgcgccg cagaacgaac tggtgcggat cggtgtcggg accgggacgg
      601 cgacgcacct tctcgacgag gttgctcagg gacgggtgct gacctcgccg ggagcggcgg
      661 tctcacccga cggtcggcgg ttcgcgctcg catggtggga cccatcggag cccgccagca
      721 agatttccgt gttcgaccgg gacgggccgc tcccgcttcg cctcgaggac cgtgtcgtcg
      781 cgtcgtaccc ggcgccgggc gcgccgggaa cgctacagtc actgacgtcg atgtcgtgga
      841 ccccggacgg gacctcgctc gtcgtctcga tgttcgcccc cttgaacgga cagatgtacc
      901 cgcctgacac gagcatccag gtgatcgaca tcgcctcgaa gtccgtgcgg cttgtcgttg
      961 cgccgcgtga cgggcattac gcgtacggtc cgatgtgggt ctcctcggac ggtcgcgcac
     1021 tgttcaagca gtggagcagt tgtgttggat gcgacggggg gcggagcggc atcgatgtca
     1081 tcgaccttgc ggacggggcg atcgtgcgga cgatcgagga cagcgtggcc ctcgggacgg
     1141 tcgatggcgt gcgctacctg ctgtcgtcgc ctgaggggct gctgagcgtg cgggggctcg
     1201 aaccccctcg cgtactgatg ccggcaaagt ggcgcgggtc gacgtggttc tcagtgcggc
     1261 cgtcgccgga cggcaaccgg ctggcggtcg cccaggcggc ggggcggacg agcccggtga
     1321 tcgcggtggc gccggacggg tcggcgcaga cggtgctcgg atacgtacgt ccggaggcga
     1381 gcgtcgtcgg cctgcgggac gcgtcgacgg cgttcgtgct ggactacgag gccgggtggg
     1441 cgtggtcttc gttcgccgac ggtgcggtca cgccgctgcc gcagcaggga gaggcgcgtc
     1501 cggaggcggc gctcgagacg gccgtggcag tctcgccaga ccgctcggtc gccgcgtact
     1561 gggtgccagg accgtcaaag cccgaggtca cgctgcagct cctcgagttg agaaccgggg
     1621 cagttacgtc ttcggtcgcc accagctcgc agatgaaggg catgtacctc gcgatgtccg
     1681 tcgatgggaa acgggtgttg tttccggacg gggacgtcct cgttgcggtg gatgtgctca
     1741 cgcgatcgac ggtccgcatc ccgctgcggc gcctcggcca ccccgccggc atcggcatcg
     1801 tctcgtccgc gacctacggc cccggcggaa ccttcgaggt cgtcgcgggc gacaagcttt
     1861 ggcgtatcga cccggcctcg ggcgatgcgc gggtcgtgac cgaccggcag ccctcgcctg
     1921 gaggctggcg gagttcggtg cagctcagcc gttcgccgga cgggcagcgc ctggtcgcgc
     1981 tgacggcgtt cgggctattc gagctgcaac tcgacggctc gtggcggctc gcgagtgggg
     2041 tggggctgga tagcagggga ccggtggcga cgtcaatcgt gtggtcgccg gactcgaggc
     2101 gcgtcgcgta ccttggcagc gagggccctg gtgtgccggg cgcactcgga atcgtggtcg
     2161 tgggcctcga tgggtcgggg gcgtacg
//
LOCUS       DAORPG010000343         2186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6087_length_2186_cov_2.966213, whole genome shotgun sequence.
ACCESSION   DAORPG010000343 DAORPG010000000
VERSION     DAORPG010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6087_length_2186_cov_2.966213"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PLX85_07600"
     CDS             complement(<1..348)
                     /locus_tag="PLX85_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurase"
                     /protein_id="HRC63069.1"
                     /translation="MASLHPRTRHAPSLPPAEAVAGRVEAIFTAPEAGAPMEQQGTVV
                     VAPGVGIVGDRYALGTGTWSDPRWPDQELTLFEAEVADALGLEPYVPRRNIVLRGVSL
                     YGLIGVRFRLGEAH"
     gene            565..1527
                     /locus_tag="PLX85_07605"
     CDS             565..1527
                     /locus_tag="PLX85_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HRC63070.1"
                     /translation="MLRHAALVLAAAVFIAAVAQVSVSLHIGPVPITGLTFGVLLSGA
                     LLGPRLGLSAALTYLAMGVVGLPVYAEGSHGWAVLTGSTGGFLIGTPFVALLVGWLAE
                     RGWDRRPATLAAALLLGNVVIYAFGLPWLWAWGGRHEALLGIDDMTLGLTLKWGLVPF
                     IPGDLAKLLLAAGLVPSGWQLLRAVRLGPARVLSGTAEAPRRAQLGLVAMGAGLAMAA
                     GAMLAWAGESDLGDGAAAVVAGMGAAGAVGALLRKRGSLGAGLTMLVGFMAAGTGGLV
                     AFVHLVRFTAAGTLELADAGVGLAVAVVAAVVLFATTAWETPAE"
     gene            1645..2019
                     /locus_tag="PLX85_07610"
     CDS             1645..2019
                     /locus_tag="PLX85_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC63071.1"
                     /translation="MARVLGIGGIFFKSPDPARLQEWYREHLGLPLEEWGGAVFRASD
                     DPEGATVWAVHAEDSDYFAPSTARFMVNFRVDDLDTILASLRAAGCEVDDKIEESTLG
                     RFGWVMDPDGHRIELWEAPPGE"
BASE COUNT          273 a          657 c          917 g          339 t
ORIGIN      
        1 gtgcgcttcg ccgaggcgga agcgcacgcc gatgaggccg tagaggctga cgcctcggag
       61 gacgatgttg cgcctgggga cgtaaggctc gaggccgagg gcgtcggcga cttcggcctc
      121 gaagagggtg agctcctggt cgggccagcg ggggtcgctc cacgtcccgg tgccgagggc
      181 gtagcggtcg ccgacgatgc cgacgccggg ggcgacgacg accgtgccct gctgctccat
      241 cggcgcgccc gcctcagggg ccgtgaagat cgcctcgacc cgaccggcaa ctgcctcggc
      301 gggcgggagc gagggtgcgt gccgcgtgcg cgggtgtaac gaagccatat caatccttcg
      361 cgtatgacgg cacgaggttg atcacgcccg tgccgctgcc gagtctcgtg gagcccgtcg
      421 acgcgatctt aacgcgggcg gcggagcatt cacgcgctcg cgacttccgg aggaggaaca
      481 gccttgttgc agagtcccgc cacgcctcac gcggccctgc cgcgcgctcg aacgctcagt
      541 cacgccgtgt ttccgcagcg gacaatgctg cgacacgcgg cgctggtgct ggcggcggcg
      601 gtcttcatcg cggcagtggc gcaggtgagc gtcagcctgc acatcgggcc ggttccgatc
      661 acagggctga cgttcggggt gctgctgagc ggggcgttgc tggggccacg gctggggctg
      721 agcgcggcgc tcacatacct cgcgatgggg gtcgtggggc tgccggtgta cgccgagggg
      781 tcgcacgggt gggcggtgct cacgggatcg acgggcggct ttctcatcgg aacgccgttc
      841 gtggcgctgc tggtgggatg gctggcggag cgcggctggg accggcggcc ggccacgctg
      901 gcggcggcgc tgctgctcgg gaacgtggtc atctacgcgt tcgggctgcc gtggctgtgg
      961 gcgtggggcg ggcgccacga ggcgctgctc ggcatcgacg acatgacgct ggggctgacg
     1021 ctgaagtggg gcctggtgcc gttcatcccg ggcgacctcg cgaagttgtt gctggcggcg
     1081 gggctggtgc cctcgggatg gcagctgctg cgcgcggtgc ggctggggcc ggcacgcgtc
     1141 ctgagcggaa cggccgaggc acctcggagg gcgcaactcg ggctcgtggc aatgggagca
     1201 gggctggcga tggccgccgg ggcgatgctc gcttgggcag gggagtctga cctcggggac
     1261 ggcgcggccg cggtggtggc cggcatggga gccgcgggag cggtgggcgc gctgctgcga
     1321 aagcgaggca gcctcggcgc ggggctgacg atgctggtgg ggttcatggc ggcggggacg
     1381 ggcggcctgg tggcgttcgt gcacctcgtg cggttcacgg cggcggggac gctggagctg
     1441 gccgacgcgg gcgtggggct cgcagtggcg gtcgtcgccg cggtggtgct gttcgcgacc
     1501 acggcctggg agacgcccgc ggagtgagcc gcctccgcgg gctgtgtgcg gccggggcgg
     1561 ttgcctcggc cgcccaattg cacctcgcca cttccatctc cggtgcacac gcgacggcct
     1621 atgatgcacc ggggaggtga ggccatggcg cgcgtgctgg gtatcggggg gatcttcttc
     1681 aagtcgccag acccggcgcg actgcaagag tggtaccggg agcacctggg gctgccgctg
     1741 gaggagtggg gcggggcggt gtttcgcgcc tcggacgacc cggagggggc gacggtctgg
     1801 gcagtgcacg cggaggactc ggactatttc gcgccgagca cggcgcggtt catggtgaac
     1861 tttcgggtgg atgacctcga cacgatcctg gcgtcgctgc gcgcggcggg atgcgaggtg
     1921 gacgacaaga tcgaggagtc gacgctgggg cggttcgggt gggtgatgga cccggacggg
     1981 caccggatcg agctgtggga ggcgccgccc ggggagtagg gtcggcttac tcctcgttcc
     2041 agtcgcagtc atcgaggatg cgctggaggc ggtcgcggac gttggcacgc acctctgagg
     2101 tatcgccgac gacgtagcgg cgccgttcgc ggatggcacg gacgacgcgg cgggcgagca
     2161 gcatcgggtc catcatctcg ggcagc
//
LOCUS       DAORPG010000344         2182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6105_length_2182_cov_3.040903, whole genome shotgun sequence.
ACCESSION   DAORPG010000344 DAORPG010000000
VERSION     DAORPG010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6105_length_2182_cov_3.040903"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PLX85_07615"
     CDS             <1..247
                     /locus_tag="PLX85_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HRC63072.1"
                     /translation="GPGTDAAIDAYNNTKNDPTLLGLFMLFGSTEKIIRQFEVQGETA
                     IGYDDKGTEVVRVPVREPSVVRDYFDRVHESYLHNIT"
     gene            297..1223
                     /locus_tag="PLX85_07620"
     CDS             297..1223
                     /locus_tag="PLX85_07620"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HRC63073.1"
                     /translation="MTSETQIPAHEQQPDVQTRRRFLQTVGGAAAAGIAIAGTIGYVA
                     TRGSGTTDKLVAGYRNGDLPDDPLNSAWLDRDAFVATMLPQNMATPLQTTVDIAELRV
                     RALHNGTDIAFHFQWDDSTDDSQESIAKFRDAVAVMLEVDPASTPSFTMGNAGAPVHI
                     MQWRASWQADVDEGHQGVKQLFPNAYNDVSPELLMPKEQAVTFYPARVAGNDQANHER
                     TSPVEEYTAIGFGSLTQHSEQKAQGHGVYRSKRWQVVISRPMAMGDKTKSEVKAGETR
                     NIAFAVWNGSANQRGGRKQYFPWAPIEVEAAG"
     gene            1220..2020
                     /locus_tag="PLX85_07625"
     CDS             1220..2020
                     /locus_tag="PLX85_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HRC63074.1"
                     /translation="MTTATALEDPRVRVAMYRSAIYEALSLGFSYPEPVLRERLIVLL
                     EDMLEFEVEVDPEMRDRARELLAALRATDIVNQAVEYNRVFDQSALCSAFETEYEADP
                     FAKARQLADISGFYNAFGMDAAQARPTTNDYIGSELEFMSLLTRNEAYATARHWTRRR
                     DVALDAQRSFLRDHLGRWERTLCGDVDRVLADDNDATAHFYRLLGALCERFVEGELRM
                     FHVKPLRLKQRMTTEREPMSCPLAPDVPEAERGSLSLGDAQALVADED"
BASE COUNT          392 a          732 c          737 g          321 t
ORIGIN      
        1 cggaccgggc acggacgcgg cgattgacgc ctacaacaac accaagaacg acccgacgct
       61 cctcggcctg ttcatgctct tcggcagcac cgagaagatc attcgccagt tcgaggtgca
      121 gggcgagacg gccatcggct acgacgacaa gggcaccgag gtggtgcgcg tgccggttcg
      181 cgagccgagc gtggtgcgcg attacttcga ccgcgtccac gagtcctacc tgcacaacat
      241 cacgtaggcg tcctcgagag ccttgaccac cgctcacgcg gaaggatgac agagcgatga
      301 cgagcgaaac gcaaatccca gcccacgaac agcaaccgga tgttcagacg cgccggcgct
      361 tcctgcagac ggtcggcggc gcggcggcgg ccggcatcgc aatcgcgggg acgatcggtt
      421 acgtcgccac tcgagggtcg gggacgacgg acaagctggt cgctggctat cggaacggcg
      481 acctgcccga cgacccgctc aactcggcct ggctcgaccg cgacgcattc gtggcaacca
      541 tgctgccgca gaacatggcc acgccgttgc agacgacggt cgacatcgcg gagttgcgcg
      601 tgcgggcgct gcacaacggc accgacatcg cgttccactt ccagtgggat gacagcacgg
      661 acgacagcca ggagagcatc gcgaagttcc gcgacgccgt cgcggtgatg ctcgaagtcg
      721 accccgccag caccccgtcc ttcacgatgg ggaacgccgg tgcgccggtg cacatcatgc
      781 agtggcgtgc gagctggcag gccgacgtcg acgaaggtca ccagggcgtg aagcagctct
      841 tcccgaacgc ctacaacgac gtatcgcccg agctgctgat gccgaaggaa caggccgtca
      901 ccttctaccc ggctcgcgtg gccgggaacg accaggcgaa ccacgagcgc acctcgcccg
      961 tcgaggagta cacggccatc gggttcggtt cgctcacgca gcacagtgag cagaaggcgc
     1021 agggccacgg cgtgtaccgc agcaagcgct ggcaggtcgt gatctcccgt ccgatggcga
     1081 tgggcgacaa gacgaagtcc gaggtgaagg ccggcgagac gcgcaacatc gcgttcgccg
     1141 tgtggaacgg cagcgccaac cagcgtggcg gccgcaagca gtacttcccc tgggcgccga
     1201 tcgaggtgga ggccgccgga tgaccaccgc aaccgcactc gaggatcctc gcgtccgagt
     1261 cgcgatgtac cgctccgcga tctacgaggc gctctcgctc ggcttctcgt accccgagcc
     1321 ggtgctgcgc gagcggttga tcgtgctcct cgaggacatg ctggagttcg aagttgaggt
     1381 cgaccccgag atgcgggatc gagcacgcga gttgctcgcc gcgctgcggg cgactgacat
     1441 cgtcaaccag gctgtcgagt acaaccgggt gttcgaccag tcggcgctct gctccgcgtt
     1501 cgagacggag tacgaggcgg acccgttcgc gaaggcccgc cagctggcgg acatctccgg
     1561 gttctacaac gccttcggaa tggatgccgc tcaggcgcga ccgacgacga atgactacat
     1621 cggctccgaa ctcgagttca tgagcctgct cacccggaac gaggcctacg cgaccgcccg
     1681 tcactggacg cggcgtcgcg acgtcgctct cgacgcgcag cggtcgttcc tgcgcgacca
     1741 cctgggacgc tgggagcgga cgctctgtgg cgacgtcgac cgcgtgctgg ccgacgacaa
     1801 cgacgcgacg gcccacttct accggctgct cggcgcactg tgtgagcgct tcgtcgaggg
     1861 cgagctgcgg atgtttcacg tgaaaccgct gcggctcaag cagcggatga cgaccgagcg
     1921 ggagccgatg agctgtccgc tggctccgga tgtgccggag gccgaacgcg ggagcctctc
     1981 gttgggcgac gcacaggcac tcgtcgccga cgaggactag accatcgagg actgcccgag
     2041 tcccctcggc ggtcccgatg tccctccaca gcgccccggg gcgacccccg gggcgctgcg
     2101 cttctctctg tacccggtca gcgggtcagt tcgctgcggt ggaccgcgct aggcgtcggt
     2161 ggcgacgggc gggtgcttct cg
//
LOCUS       DAORPG010000345         2182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6108_length_2182_cov_1.566055, whole genome shotgun sequence.
ACCESSION   DAORPG010000345 DAORPG010000000
VERSION     DAORPG010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6108_length_2182_cov_1.566055"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..117)
                     /locus_tag="PLX85_07630"
     CDS             complement(<1..117)
                     /locus_tag="PLX85_07630"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005345823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRC63075.1"
                     /translation="MPADLRHFKALTFGHPVLMGRRTFEAIGRPLPGRRNLVL"
     gene            complement(63..248)
                     /locus_tag="PLX85_07635"
     CDS             complement(63..248)
                     /locus_tag="PLX85_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63076.1"
                     /translation="MERVQRFVLAVIVALLTFSASGAATLLVVEPCSAYDLSGKDDGT
                     CPPTCAISRRSLSAIRC"
     gene            complement(293..790)
                     /locus_tag="PLX85_07640"
     CDS             complement(293..790)
                     /locus_tag="PLX85_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC63077.1"
                     /translation="MRIGTLAQRTGTNVPTIRYYESVGLLPRAARQEGGHRLYMDADV
                     DRLTFIRRCREFGFSMEQVRSLAALAQDERRSCLELRAVAATQLASVQARITELQGLA
                     RSLQGFVTTCDATCVGGPGPECTILDDLKKTTPNCACGPRQPPLARLSGRRPPVRPKS
                     RTGGR"
     gene            858..1172
                     /locus_tag="PLX85_07645"
     CDS             858..1172
                     /locus_tag="PLX85_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63078.1"
                     /translation="MATDSPIACTLSPSDYRARLATIADLRRDALRHVEQRGCTIELH
                     YAPEAAARVRTLVEKERTCCAFLSFDLREEPDAVRLLVTVPAAAVEVVPELLDELTGR
                     VR"
BASE COUNT          346 a          710 c          744 g          382 t
ORIGIN      
        1 cagcaccagg ttgcgccgcc ccggcaacgg ccggccgatg gcctcgaagg tgcgccgccc
       61 catcagcacc ggatggccga aagtgagcgc cttgaaatgg cgcaggtcgg cgggcaggtg
      121 ccgtcgtcct tgccactcag gtcgtacgcc gagcagggct cgacgacgag gagggtggcg
      181 gctccagacg cactgaaggt caggagcgcg acgatgactg cgaggacgaa tcgctgcact
      241 cgttccatct tacactgctg cggtctcggc ccagctgagg tggcagtctg actcagcgcc
      301 cccctgtccg tgatttggga cgcacaggcg gccgccgacc ggacaggcgc gcgagggggg
      361 gctgacgcgg accgcacgcg cagttcggcg tcgtcttctt caaatcgtcg aggatcgtgc
      421 actccggtcc gggcccgccg acacacgtgg cgtcgcaggt ggtgacgaac ccctggaggc
      481 tccgtgccag accctgcagc tccgtgatgc gcgcctgaac gctcgccagc tgcgtggcag
      541 ccacagcgcg tagttccaag cacgagcgac gctcgtcctg cgcgagcgcc gctaacgaac
      601 gcacctgctc catggagaag ccgaactcgc gacagcgccg gatgaacgtg aggcggtcga
      661 catcggcgtc catgtaaagg cggtgccccc cttcttgtcg ggcggctctc ggaagcaggc
      721 cgacggactc gtagtatcga atcgtcggga cgttggtgcc ggtgcgttgg gccagggtgc
      781 cgatgcgcat ggattcctcg tcgagggctt gaacctcaag tgacttgagg ttgtagcgta
      841 gtcgttgagg aggcccgatg gctactgaca gtccgattgc ctgcaccctg tcgcccagcg
      901 actaccgcgc cagactggcg acgatcgccg acctgcgtcg ggacgcgctc cgccacgtcg
      961 agcagcgagg gtgcacaatt gaactgcact acgcgccaga ggctgcggcg cgggtgcgaa
     1021 cactggtgga gaaggagcgt acctgttgcg cgtttctgag ctttgacctg cgggaggaac
     1081 cagatgcagt gcggctgctg gtgacggtgc cggcggccgc cgttgaggtg gtgcccgagc
     1141 tgctcgacga actgacggga cgggtgcgat gagaccgctg ccagtgctca cgcgtccacc
     1201 ggcgcgaccg acgtcgacgg cagcgacgac cctcgcaatc ggcgctgtcg cgtgtggggt
     1261 gtgctgcgcc ttgccgttcg cggctccggc cgtggcgctc gctacgtcag gcggcctgat
     1321 cgcattcgtg gcgcggacgt tcgcgtgggc actctatccc gcgttggcca tggtgattgg
     1381 cgggtgggtg tgggtcgctc tcgatagccg ccgtcgaggc aggcgtccgg ctcgttccac
     1441 tatgctgatg ctgtcgctgg cgacggtcgc gctctccgcc gccttggtgt ggcctgtgag
     1501 atgaggccca gctcacatcc atccccggtc gttcaggaga gattgacgca gtcgtcgata
     1561 ccatgtctca tacacctgcc gagtcggcgt caaaccctgc cgagcgacct cttgcgttcc
     1621 tgacgcgcag cggctgcgcc aaaactcgcg tcatgcggca acgccttgac gagtcgctgc
     1681 gaagtctccg gctacgagag aagtacgacg ttgtcgacgc ggacagcctt cctgagaacg
     1741 acagccgccg cggctatggg acgccaacca ttctcttcaa gggcaaagat ctgttcggat
     1801 tgtccgagcc aaatccgccg ctcccgtctc caacttgaag gctctatccg ggcggagttc
     1861 cgtccgtcga ggctctcaag gcagcgctgc tgagcgcgca aggccggtag cgttggtgcc
     1921 ggcttgaccg tcagaaccat ctggcgtggc ctcaccgaat caagccccgc gctcgcgctg
     1981 gccgatacga gccctgcggg acgccgggat tcggttcacc gcgacctgaa cttcggcccc
     2041 tggcgaccgg agcggcaccc gggttcgtcg ggagctgcgc gcgccccgta agctccaggc
     2101 cgcatggtgc gttcatctgt ttatggatgg cgccattgcg tatcgcgcgt gcctcgccgc
     2161 tgagggtcgc caaccacggc cc
//
LOCUS       DAORPG010000346         2181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6114_length_2181_cov_2.536689, whole genome shotgun sequence.
ACCESSION   DAORPG010000346 DAORPG010000000
VERSION     DAORPG010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6114_length_2181_cov_2.536689"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /locus_tag="PLX85_07650"
     CDS             <1..1304
                     /locus_tag="PLX85_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRC63079.1"
                     /translation="LPAGEVVAASVSGHNDYQDGSAFLASGGQRGPQFDLLRPGTYYV
                     NPLMFEVKVDDVAVVQRGEVAVLVSNVGPEPSAPTTERLAAGEERYVVPSGFRGIQAQ
                     VAGPGVYYLNRWAYLAYVIPTTNLTIDWADETNVTDEPQQADPKADPREILGRRIQLF
                     NPLSVISRDGFEMRVSVKVIMRVHPEQAPLMVAKIGSVQNLIDHVIHPMIDSSFRNQA
                     SSSEAMAFMQDRALEQEKAEKRAREEMEQYHVECVRVLISQIILPEELMAIQTRRVIA
                     TQQQEMFVAQQQSEQQRIATENTRAQADQQVNLVSAQIGVQIAEQTRQKTVIEAEGRA
                     RAVELEGNAEGTKILAIGQATAEAYDRQQEAIGQANLFGIEVAKALAAAGLKITPDIV
                     VGGGADGGGANIFGAFLAQMLANNRTSLNGSDQNPAATSTS"
     gene            complement(1342..1428)
                     /locus_tag="PLX85_07655"
     tRNA            complement(1342..1428)
                     /locus_tag="PLX85_07655"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1392..1394),aa:Leu,seq:caa)
     gene            complement(1453..1527)
                     /locus_tag="PLX85_07660"
     tRNA            complement(1453..1527)
                     /locus_tag="PLX85_07660"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1493..1495),aa:Gly,seq:gcc)
     gene            complement(1588..>2181)
                     /locus_tag="PLX85_07665"
     CDS             complement(1588..>2181)
                     /locus_tag="PLX85_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRC63080.1"
                     /translation="RRTTVPAVRTIADGVGVAGPSELTLSLIERYVDDLVEVPEDAIA
                     RAVVLLIERTKLVVEGAGALGVAALLTGVAEARGRTVAVLSGGNIDINLLDRIVERGL
                     LDEGRRQRLTFAAANVPGELARITNALAAGGANIIEVAHELVVPGLPVGVARITVRLD
                     VAGPEGLTRLEVALLEAGLQRAIETDFATDAAAAMPS"
BASE COUNT          395 a          798 c          675 g          313 t
ORIGIN      
        1 cgctgccggc cggtgaggtc gtcgccgcca gcgtctccgg ccacaacgat taccaggatg
       61 gctccgcgtt cctcgcgagc ggtggccagc gtgggccgca gttcgacctc ctgcgacccg
      121 gcacctacta cgtgaatccg ctcatgttcg aggtcaaagt cgacgacgtc gccgtggtcc
      181 agcgtggcga ggtcgcggtc ctcgtctcta acgtcggacc cgagcccagc gccccgacca
      241 cggagcgcct cgccgccggc gaagaacgtt atgtcgtccc cagcggattc cggggcatcc
      301 aggcgcaggt cgccggaccc ggcgtctact acctgaaccg ttgggcctac ctcgcttacg
      361 tcatccccac caccaacctc accatcgact gggccgatga gacaaacgtg accgatgagc
      421 cgcagcaggc cgacccgaag gcggatccac gcgagatcct cggccggcgc atccagctct
      481 tcaatccgct atccgtcatc agtcgggacg gctttgagat gcgcgtgtcg gtgaaagtca
      541 tcatgcgcgt gcatcccgag caggccccgc tcatggtcgc gaagatcggc tcggtccaga
      601 acctgatcga tcacgtcatc cacccgatga tcgactcgtc attccgcaac caggcctcgt
      661 cgagcgaggc aatggcgttc atgcaggacc gcgcgctcga gcaggagaag gcagaaaagc
      721 gagcccggga ggagatggag cagtaccacg tcgaatgcgt gcgtgtcctg atctcgcaga
      781 tcatcctgcc cgaggaactc atggcgattc agacgcgccg cgtcatcgcg acgcagcaac
      841 aggagatgtt cgtcgcgcaa caacagtccg agcagcagcg catcgccacc gagaacaccc
      901 gcgcgcaggc ggatcagcag gtcaacctcg tcagtgcgca aatcggggtc cagatcgccg
      961 agcagacacg gcagaagacg gtcatcgaag ccgaggggcg ggcgcgcgcc gtcgaactcg
     1021 aaggcaacgc cgagggcacc aagatccttg cgatcggtca ggcgaccgcc gaggcgtacg
     1081 accgacagca ggaggcgatc gggcaggcca acctcttcgg gatcgaggtg gccaaggccc
     1141 tcgccgcagc cggcctcaag atcacgccag acatcgtcgt cggcggcggc gctgacggtg
     1201 gtggggcgaa catcttcggc gcgttcctcg cacagatgct cgcgaacaac cgcaccagcc
     1261 tgaacggatc ggaccagaac cccgccgcta cctcgacgtc atgaggtcct agctgcattc
     1321 gagactggct acgagcagca gtggtgccga aggccggggt cgaaccggca cggggttgcc
     1381 cccaacggtg tttgagaccg tcgcgtctgc ctattccgcc acttcggcct cgaggacgga
     1441 cgagaggagg agtggagcgg aagacgaggg tcgaactcgc gacctcctcc ttggcaagga
     1501 ggcgctctac cactgagcta cttccgcgtg ccgatgagta tagccagcgc cccgtcgcga
     1561 cggcaacgcg accgggccaa tcgagcccta cgagggcatc gccgccgccg cgtccgtcgc
     1621 gaagtccgtc tcaatcgccc gctgcagccc cgcctccagc agcgccacct cgaggcgcgt
     1681 cagaccctcc ggccccgcca cgtccagccg cacggtgatg cgtgccaccc ccacgggcag
     1741 ccccggcacg accagctcgt gcgccacctc gatgatgttc gccccgcccg ctgccagcgc
     1801 gttcgtgatg cgcgccagct cccccggcac gttcgccgcc gcgaacgtca gccgctgccg
     1861 tcgcccctca tcgaggagcc cgcgctccac gatccggtcc agcaggttga tgtcgatgtt
     1921 cccaccggat agcacggcca ccgtgcgccc gcgcgcctcg gcgacccccg tcagcagcgc
     1981 agcgacgccg agcgcgcccg ccccctccac caccagcttc gtccgctcga tcaacagcac
     2041 caccgctcgc gcgatcgcgt cctcgggcac ctccacgagg tcatccacgt atcgctcgat
     2101 gagcgacagc gtcagttccg agggcccggc gacgcccacg ccgtccgcga tcgttcgcac
     2161 ggcgggcacg gtcgttcgtc t
//
LOCUS       DAORPG010000347         2180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6118_length_2180_cov_3.552941, whole genome shotgun sequence.
ACCESSION   DAORPG010000347 DAORPG010000000
VERSION     DAORPG010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6118_length_2180_cov_3.552941"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1313)
                     /locus_tag="PLX85_07670"
     CDS             complement(120..1313)
                     /locus_tag="PLX85_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-2-enoyl-CoA reductase family protein"
                     /protein_id="HRC63081.1"
                     /translation="MALQVVSPRIRGFIATNAHPDGCALNVRRQIEVARGLRGSTQRY
                     RNVLVIGSSTGYGLASLLSAAFGLDANVLGVCFERESTEDRTGSAGWYNLAEAHRVAR
                     EENRLVRTINGDAFSHAVKQQAVDALRAEFGPLDLVVYSLAAPRRQDADSDTVWSSVL
                     KPIGAPYTGKTIDLRNDAVVETTIEPATDDEIEATVKVMGGEDWAAWLHTLADAGLLA
                     PGCRTVAYSYIGPEVTYPIYRSGTIGRAKEHLEASATALTAMLKDRCQGAAWVSINKA
                     VVTQASAAIPAVALYMSIAFKLMKERGTHEGTIEQIVRLLREHLNLDVPLVDEEGRIR
                     IDDWEMQPDLQAEIAAIWDTITTDTLTEVSDYAGYKRDFEQLFGFSVPGIDYAAPTEV
                     HRLIQ"
     gene            1445..2011
                     /locus_tag="PLX85_07675"
     CDS             1445..2011
                     /locus_tag="PLX85_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63082.1"
                     /translation="MQWRLGVALTILVISVALGACGGGDDKPADPATWAAGMCTAEQK
                     FVTAIIESRDNRDPTTLDLQERKERAARLGKIEADAARQLAKDLKAITPPKDAKAYHD
                     ALIQQAEDTAKAVDTQVQAINKATTAQQIAVANAEAQFESRGAAQEVRAKGVSLTDDL
                     VEVLLNQEECGRAPIPGEPDQAEPTPSA"
     gene            complement(1995..>2180)
                     /locus_tag="PLX85_07680"
     CDS             complement(1995..>2180)
                     /locus_tag="PLX85_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593665.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HRC63083.1"
                     /translation="IVARETVRAMRKNVLAKCYGGDVTRKRKLLEAQKAGKKRMKMLG
                     SVEVPQEAFMAVLKLRE"
BASE COUNT          363 a          671 c          799 g          347 t
ORIGIN      
        1 tcgaagaggc gctcgacgag gtcggtggcc tggtcctcgg gcgtctcggg catcgggttg
       61 ctccaatcgt gccgtgttgg cgtcgaatgt agccgacacg gcaacgacgc gagcgggagc
      121 tactggatga gccggtgcac ctcggtcggg gcggcgtagt cgatccccgg gacggagaag
      181 ccgaagagct gctcgaagtc acgcttgtag ccggcgtagt cgctgacctc ggtcagggtg
      241 tcggtggtga tggtgtccca gatagcggcg atctcggcct gcaggtcggg ctgcatctcc
      301 cagtcgtcga tgcggatgcg gccctcctcg tcgacgagcg ggacatcgag gttcaggtgt
      361 tcgcgaagga ggcggacgat ctgctcgatg gtgccctcgt gggtgccgcg ttctttcatc
      421 agcttgaagg cgatgctcat gtagagggcg acagcgggaa tcgcggccga cgcctgcgtg
      481 acgacggcct tgttgatgct cacccaggcg gcgccctggc agcgatcctt gagcatcgcc
      541 gtgagggcgg tggcacttgc ctcgaggtgc tccttggcac ggccaatggt gccggagcgg
      601 tagatggggt aggtcacctc ggggccgatg tatgagtagg cgacggtgcg gcagccgggg
      661 gcaagcaggc ccgcgtcggc gagtgtgtgc agccaggccg cccagtcctc gccgcccatc
      721 actttgacgg tggcctcaat ttcgtcgtcg gtggccggtt cgatcgtcgt ttcgacgacg
      781 gcatcgttac gcaggtcgat ggtcttgccg gtatacggcg cgccgatggg cttcagtacg
      841 ctgctccaga ccgtgtcgct gtccgcatcc tgccgccgtg gcgcggcaag ggagtacacc
      901 acgaggtcga gcgggccgaa ctcggcgcgc agcgcgtcga cggcttgctg cttgacggcg
      961 tgcgagaagg cgtcgccgtt gatcgtgcgg acgaggcggt tctcctcgcg ggcgacgcgg
     1021 tgggcttcag caaggttgta ccagccggcg gatccggtgc ggtcctcggt ggactcgcgc
     1081 tcgaagcaga cgccgaggac gttggcgtcg aggccgaagg cggcgctgag caggctcgcc
     1141 aggccgtagc cggtgctcga cccgatcacg aggacgttcc gatagcgctg ggtactgcct
     1201 cgaaggccgc gcgcgacctc gatctggcgg cggacgttca gcgcgcaccc gtcggggtgg
     1261 gcgttggtgg caatgaaccc gcggatgcgc ggggaaacaa cctgtagagc caagcctgag
     1321 cctccgatcc gagcgctacg gtagcagcac ccgcagagat gggtcgatga agcccggcgg
     1381 tacccatctc aggcgctgag atggcacaat cacggctcga acacggcagg gcagggggat
     1441 agccatgcag tggcggctgg gcgtcgcgct cacgatcttg gtcatctcgg tggcgctggg
     1501 ggcctgtggc gggggcgacg acaagcccgc cgacccggcg acctgggcgg ccggcatgtg
     1561 tacggccgag cagaagttcg tgaccgcgat catcgagtcg cgggacaacc gagacccgac
     1621 caccctcgac ctgcaggaac gcaaggaacg ggcggctcga ctcgggaaga tcgaggcaga
     1681 cgccgcgcgc cagctcgcga aggacctgaa ggcgatcacg ccgccgaagg acgcgaaggc
     1741 ctaccacgac gcactgatcc aacaggccga ggacacggcc aaagcggtcg acacgcaggt
     1801 gcaggcgatc aacaaggcca cgacggcgca gcagatcgcc gtcgccaacg ccgaggcgca
     1861 gttcgagtcg cgcggagcgg cccaggaggt acgggcgaag ggagtgtccc tgaccgacga
     1921 cctcgtcgaa gtgctgctga accaggagga gtgcggacgc gcgccgatcc cgggcgagcc
     1981 cgatcaggcg gagcctactc cctcagcttg aggacggcca tgaaggcttc ctgaggcacc
     2041 tcgacggagc ccaacatctt catgcgcttc ttgccggcct tctgcgcctc gagcagcttt
     2101 cgtttgcggg tgacgtcgcc gccgtaacac ttcgcgagca cgttcttgcg catggcgcgg
     2161 accgtctcgc gggcgacgat
//
LOCUS       DAORPG010000348         2173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6152_length_2173_cov_3.883853, whole genome shotgun sequence.
ACCESSION   DAORPG010000348 DAORPG010000000
VERSION     DAORPG010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6152_length_2173_cov_3.883853"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PLX85_07685"
     CDS             complement(<1..841)
                     /locus_tag="PLX85_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HRC63084.1"
                     /translation="MPRSIWRGAISFGIVSIPVRLFTATQDKDISFRMLHEKDNARIR
                     FIRICSEEETEVSNDEIVRGYEYEKDHYVVLTDEDFDQLPLPSKHTIDLTAFVEAEEI
                     DPVFYEKSYYLEPEDTGVKPFALLMRALQEKGLTAVAKIAIRNKEQLCALRPMDGTLM
                     LETLFYPDEIRVERGAEVPEVSVSDQELQMASALIDLLTEQFDPAKYQDTYREALMQV
                     IDAKLEGQEVVEAEAPAPTKVVDLMAALKASVEATRRQKEAQAGSARDEDAEATTTAS
                     SRRR"
     gene            complement(956..>2173)
                     /locus_tag="PLX85_07690"
     CDS             complement(956..>2173)
                     /locus_tag="PLX85_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HRC63085.1"
                     /translation="ALDLGDVGRGLAMLEAGGYVLRGRFTPGLPDEEFCDRRLLARIH
                     RYTLDQLRREIDPVTTRDYMRFLLSWHHVAPGSRMKGRTAVRHAVARLEGFEAAASAW
                     ESELLAARVDDYTPAWLDELCLAGEVTWARLTPRRAVGGGTVAASRVTPMTLALRSDL
                     PVLLEAVRGSEEDSTRPETGAAAELLELLEARGALFFDELVAGTRRLATDVEQGLREL
                     VARGLVASDGFQGLRQLSGLWRRSGRGRRGAGYLRAAGGPPGRWAVVAAPSTGTHDLE
                     SLAEEVANVLLQRYGVVFRDVVAQESFTVPWREVLRALRRFEARGSARGGRFVSGFVG
                     EQYALPEAVEALRRVRKDPLTGERVRIAAVDPLNLTGVLFPGPRIPSVAGRTVDYVDG
                     IPELEPVLVASRS"
BASE COUNT          348 a          671 c          822 g          332 t
ORIGIN      
        1 gacggcgccg gctggacgcg gtggtcgtcg cctcggcgtc ctcgtctcgc gcggagccgg
       61 cctgcgcttc cttctggcga cgggtcgcct cgacgctcgc cttgagggcg gccatgaggt
      121 cgacgacctt ggtgggtgcg ggcgcttccg cctcgacgac ctcctggccc tcaagcttgg
      181 catcgatgac ctgcatcaac gcctcccggt aggtgtcctg gtacttcgcg gggtcaaact
      241 gctcggtcag gaggtcaatc agcgcggacg ccatctggag ctcctggtca ctcacactta
      301 cctcgggaac ctcggcgccg cgctcgacgc ggatctcgtc gggatagaac agggtctcga
      361 gcatgagggt tccgtccatg gggcggagcg cgcagagttg ctctttgttg cggatggcga
      421 tcttggcgac agcggttagg cccttctcct gcagcgcgcg catgaggagc gcgaagggct
      481 tcacccccgt gtcctcgggt tcgaggtagt agctcttctc gtagaagacg gggtcgattt
      541 cctcggcctc gacgaacgcg gtcaggtcga tggtgtgctt actggggagc ggaagctggt
      601 cgaaatcctc gtcggtgagg acgacataat gatccttttc gtactcgtag ccgcgcacga
      661 tctcgtcgtt ggagacttcg gtctcctctt ccgagcagat gcggatgaag cggatacggg
      721 cgttgtcctt ctcgtggagc atgcggaagg agatgtcttt gtcctgggtg gcggtgaaga
      781 ggcgaacggg gatgctgacg atgccgaagc tgatcgcacc tcgccagatg gaacgtggca
      841 tggggccgac tccgttgttg gagccgatgc gctcgcatcg gccggggacg ccgaatggag
      901 tacgcattat aggggacgga ttacgacaag ctgcactgcc gaaatggcgc gggggttacg
      961 agcggctcgc gaccaggacc ggttcgagct cagggatgcc gtcaacgtag tcgacggtgc
     1021 ggccggctac cgaagggatt cgcggacccg ggaataggac gccggtgaga ttgagcgggt
     1081 cgaccgccgc gatgcgtacg cgctcgccgg tcagcgggtc cttgcggacg cgccggaggg
     1141 cctcgacggc ctcgggaagg gcgtactgtt cgccaacaaa gccgctcacg aagcggccac
     1201 cgcgggccga gccgcgggcc tcgaagcggc ggagggcgcg caggacttcg cgccaaggca
     1261 cggtgaacga ctcctgcgcg acgacatcac ggaagacgac gccgtagcgc tgaaggagga
     1321 cgttcgcgac ctcctcggcg agggactcga ggtcgtgagt acccgtcgaa ggcgcggcga
     1381 ccaccgccca gcggccgggc ggtcccccgg cggcgcggag gtagcccgca cctcgacggc
     1441 cacggcctga gcggcgccag agtccggaca gctgacgcag gccctggaag ccatcggaag
     1501 cgacgagacc ccgggcgacc agctcgcgga ggccctgctc aacgtcggtg gcgagccggc
     1561 gggtgccggc cacgagctcg tcgaagaaga gcgcgccgcg cgcctcgagg agttcgagga
     1621 gttcggccgc ggctccagtc tccggccggg tggagtcctc ctcggagccg cggacggcct
     1681 cgaggaggac ggggaggtcg gagcggaggg cgagggtcat cggggtgacg cgcgaagcgg
     1741 cgacggtgcc gcccccgacg gcgcggcgag gagtaagacg ggcccaggtg acctcgccgg
     1801 cgaggcaaag ctcatcgagc caggcgggag tgtagtcgtc tacgcgggcg gcgagcagct
     1861 cgctctccca tgcgctcgcg gcggcctcga agccctcgag gcgggcgacg gcgtgacgca
     1921 cggccgtgcg gcccttcatg cgggagccgg gggccacgtg gtgccagctc agcaggaagc
     1981 gcatgtaatc gcgggtggtg acgggatcga tctcgcgtcg gagctggtcg agcgtgtagc
     2041 ggtggatgcg cgcgagcagt cggcggtcgc aaaactcctc atccggcagg cccggcgtga
     2101 agcgcccgcg gaggacgtaa ccgccggcct cgagcatcgc gaggccgcgg ccgacgtcgc
     2161 cgaggtcgag ggc
//
LOCUS       DAORPG010000349         2173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6153_length_2173_cov_3.307365, whole genome shotgun sequence.
ACCESSION   DAORPG010000349 DAORPG010000000
VERSION     DAORPG010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6153_length_2173_cov_3.307365"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLX85_07695"
     CDS             <1..447
                     /locus_tag="PLX85_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRC63086.1"
                     /translation="TDGELTASRDLLGAIVREVRAFRPYAVYTHDPEPVVHNEGFINH
                     SDHRATGLATVDAVYPAARDRLNFPEQVAEGLAPHVVRELYLWGSNQPNCDVDISSVV
                     EAKIEALLLHGSQFNNDDGFIDYMRSRWQDAEGRSFEHFRRVVMFG"
     gene            444..947
                     /locus_tag="PLX85_07700"
     CDS             444..947
                     /locus_tag="PLX85_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRC63087.1"
                     /translation="MTPSGAGSVSGHAFCPVCGTALDADAGPGGRPACPRCGFVRYDN
                     PKVATGVVAECDGQILLVRRNHEPMLGRWSFPSGFVDAGEVVPEAAIREVFEETGVEV
                     RIDRLLGVYSRHGDPVVFVAYAGSVTAGTPVAGPEAFEVGLFPPDALPDLAFPHDDAI
                     VAAWRAG"
     gene            1004..1597
                     /locus_tag="PLX85_07705"
     CDS             1004..1597
                     /locus_tag="PLX85_07705"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HRC63088.1"
                     /translation="MTSSPDPSPAARPPVLSLLQRRGIEAEVLIPFIRRLEAELGLER
                     AHELARETIAEIAREQGRAVAEGLGRSDLEGFHEVRDTWSGAGGDLTIETLREDGERL
                     DFNVTRCRFAEMYERLGARDLGFLLSCGRDFTLSEGYSTDLALERTQTIMQGASFCDF
                     RYRYHPSTSATAGLPADGAASDNAAGTAASGSTASPR"
     gene            1644..1988
                     /locus_tag="PLX85_07710"
     CDS             1644..1988
                     /locus_tag="PLX85_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63089.1"
                     /translation="MRIQIYGDDREPILERTRELVDEAVAQAGLADVEIELIAIHNDD
                     EAKAQRSLGAPTVRIDGFDAEYAEREPPETTAGARYYSTPDGWQRLPERGMIVFAIKE
                     AQARAARRQAAS"
BASE COUNT          316 a          818 c          741 g          298 t
ORIGIN      
        1 accgacggcg agctgacggc gtcgcgcgat ctgctcggcg cgatcgtccg cgaggtccgt
       61 gccttccgcc cctatgccgt ctacacgcac gaccccgagc ccgttgtcca caacgagggc
      121 ttcatcaacc acagcgacca ccgcgcgacc ggcctcgcca ccgtcgatgc cgtctacccg
      181 gccgcacgtg accggctgaa cttccccgag caggtcgccg agggcctggc cccccacgtc
      241 gtgcgggagc tgtacctctg gggctcgaac cagccgaact gtgacgtcga catctccagc
      301 gtcgtcgagg cgaagatcga ggcgctcctg ctccatggca gccagttcaa caacgacgac
      361 gggtttatcg actacatgcg ctcgcgctgg caggacgccg aaggccgctc cttcgagcac
      421 ttccgccgcg tggtgatgtt cgggtgacgc cgtccggggc gggctccgtt tccggccacg
      481 ccttctgccc ggtctgtggc acggccctcg acgcggacgc cggcccggga gggcggcccg
      541 cctgcccgcg ctgcggcttc gtgcgctacg acaacccgaa ggtcgccacg ggcgtggtcg
      601 ccgagtgcga cggccagatc ctgctcgtgc gccgcaacca cgagcccatg ctcggccgct
      661 ggtcgttccc ctcgggattc gtggatgcag gcgaggtcgt gcccgaggcc gccatccgtg
      721 aggtgttcga ggagaccggc gtggaggtgc gcatcgaccg cctgctcggc gtgtacagcc
      781 gccacggcga cccggtcgtc ttcgtcgcct acgccggctc ggtcacggcc ggcactcccg
      841 tcgccggtcc cgaggcgttc gaggtcggcc tcttccctcc ggacgcgctg cccgacctcg
      901 ccttcccgca tgacgacgcc atcgtcgccg cctggcgcgc cggctgaccc tgccgcacgg
      961 tacgctcagg ggagcaccca atagcgacgc gaagggagcg cgcatgacct cgtcgcctga
     1021 tccgtccccg gcggcccgcc ctcccgtgct ctccctgctc cagcgccgcg gcatcgaggc
     1081 cgaagtcttg atccccttca tccgccgcct cgaggcggag ctcggcctgg agcgggcgca
     1141 cgagctggcg cgcgagacga tcgcggagat cgctcgggag cagggccgcg cggtggcgga
     1201 ggggctcggc cgcagtgacc tcgaggggtt ccacgaggtg cgggacacct ggagcggcgc
     1261 cggaggcgac ctgacgatcg agacgctgcg cgaggacggc gagcgcctcg acttcaacgt
     1321 gacccgctgc cgcttcgccg agatgtatga acgcctcgga gcgcgtgacc tcggcttcct
     1381 gctctcctgc gggcgcgact tcacgctctc cgagggctac tccaccgacc tcgccctgga
     1441 gcgcacgcag accatcatgc agggcgcctc cttctgcgac ttccgctatc gctaccatcc
     1501 ctcgacctcg gccacggcgg gactccccgc agacggcgcg gccagcgata atgccgcggg
     1561 gacggccgcc tccggctcga ccgccagtcc gcgctgaccg ctgcctcgag gtcgcctcgg
     1621 ggcgaccgca gaaagaggaa ccgatgcgta tccagatcta cggcgacgac cgcgagccca
     1681 tcctcgagcg cacccgcgag ctggtcgacg aggccgtcgc gcaggcgggt ctcgccgacg
     1741 tggagatcga gctgatcgcg atccacaacg acgacgaagc gaaggcgcag cgcagcctcg
     1801 gcgcgcccac ggtgcgcatc gacggcttcg acgccgagta cgccgagcgc gaaccaccgg
     1861 agacgaccgc cggcgcgcgc tactacagca cccccgacgg ctggcagcgc ttgccagagc
     1921 gcggcatgat cgtctttgcg atcaaggaag cgcaggcccg tgccgcgcgg cggcaggcgg
     1981 ccagctgagc ccacaggggg ccgtatgagc ggcgacgatc ggccgggcga tacgcccact
     2041 cccgacagta ccaacgagac gccacccgag gcgcccgagc cgccgcctga actgagcccg
     2101 cgccgcccac cgttgcgagg gacgggcgcc ggcttcggtg cggcgggcga gggtgggggc
     2161 gcttcgcggc tga
//
LOCUS       DAORPG010000350         2172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6159_length_2172_cov_3.393009, whole genome shotgun sequence.
ACCESSION   DAORPG010000350 DAORPG010000000
VERSION     DAORPG010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6159_length_2172_cov_3.393009"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(415..810)
                     /locus_tag="PLX85_07715"
     CDS             complement(415..810)
                     /locus_tag="PLX85_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRC63090.1"
                     /translation="MTLSLEDRLDILDLAARYSHASDHGDGRALADTFTEDGIFEGAG
                     EPRRGRAAHEAATNALAASGLVLRHFVSNPVIEGDGDRATMRLYVEVKNLADPTTPML
                     VGCYYDELQRIDGRWKFARRRVETDYPQA"
     gene            968..2158
                     /locus_tag="PLX85_07720"
     CDS             968..2158
                     /locus_tag="PLX85_07720"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC63091.1"
                     /translation="MAAGNTRSEVRGATDEGPLPRWGALAYPNYRNYWIANLVRIFGI
                     QFRFIGGLWLVQQLADSPLWLGVLSISTAVPTIVLSVPAGALADRVDNQKLLVWSSAL
                     TAVVTGALAVAVVLDVANVWIAVLWAAVLGALMALANPAQSAILPRLIDMRAIASAVA
                     YTSAAWSGMRIVGPFAVGILIAVIGTGQAFIVTAAAFAISALLLQRLRLAPVERRGTH
                     AEHDGGMLEGARYIFRNKLFLATIGLSFFTSVFGSSYIVLLPTFADDILHVGARGFGL
                     METAAGMGALLGTFLIVRVRSQRHYGPVMLLAAAAFGILIAGFAASRTIGVSMALLFG
                     GGLASAVYLNVGMTTLQVLVPDELRGRVMGVWSMTYFLGSVGGLPAGLVAAWLGAPVA
                     VASW"
BASE COUNT          292 a          765 c          703 g          412 t
ORIGIN      
        1 agcacctgca ccctgactcg gtgcgcgact cgttcctgcg ctagccggcc gcgcttgagg
       61 cggcccgaca caaagcggcc ccggttcccc ggggccgctg tcactgtgtg ccaattgagt
      121 ggtggtgctc gaagcgcttc gccggcctga gtaccgaggg cagtcgcaat gcgattgcct
      181 gtgctcgtgg ctccgaggct accgtgccgc cgctactacg tagtccaagg gcggggccct
      241 cctcctagtg cttgatcagc catcgcagca atcctgcgtg aggaagttct gctttcagcc
      301 aaaccccctt cccgacggtg gtttcggggt ggatccaccg caatcatcat gctccagcca
      361 cgcaagccca cacagggtcg aaggtcacgt gactttcgag gtcgcccgat ccgctcaggc
      421 ttgcgggtag tccgtctcga cgcgacgccg ggcgaacttc cagcggccgt ctatgcgttg
      481 cagctcgtcg taatagcagc caacgagcat tggagtcgtg ggatccgcga ggttcttcac
      541 ctctacgtac agcctcatcg tcgcccggtc gccgtctccc tcgatcaccg ggttgctgac
      601 gaagtggcgc agcacgagtc cgctcgccgc cagcgcgttc gtcgcggctt cgtgagccgc
      661 ccgtccgcgt cgaggttcgc ccgcgccctc gaagatccca tcctccgtga acgtgtccgc
      721 caacgcccga ccgtcaccgt ggtcgctggc gtggctgtac cgcgcggcga gatcgagtat
      781 gtcgagccgg tcctcgagac tgagcgtcat gatggtctcc tcacgaaact gcttcgctgc
      841 cctcggcgga ttctatgtcg cccggcctgc ggcgggggtg gcagcgactc gcgacgatgg
      901 caaaggcacg cgaacatgta cagtgtcact tacaggtact ggctgcgagg cacaggcggg
      961 ggcaaggatg gcggctggca acacgcgatc tgaggtacga ggcgcgaccg acgaagggcc
     1021 gttgcctcgc tggggcgcgc tcgcctaccc gaactatcgc aactactgga tcgcgaacct
     1081 cgtccgcatc ttcggaatcc aattccgatt catcggaggg ctctggctcg tccagcagct
     1141 tgctgactcg cccctctggc tcggcgtact cagcatttcg accgccgtcc ccaccatcgt
     1201 gctctccgtg cccgccggcg cgctcgccga ccgcgtcgat aaccagaagc tcctcgtctg
     1261 gtcgagcgcg ctgaccgccg tcgtcaccgg cgccctggcg gtcgccgtcg tgctcgatgt
     1321 cgccaacgtc tggatcgccg tgctgtgggc ggccgtcctc ggcgcgctga tggccctcgc
     1381 caacccggcg cagagcgcca tcctgccgag gctgatcgac atgcgcgcca ttgccagcgc
     1441 ggtcgcctat acgagcgctg cctggagcgg gatgcgtatc gtcggcccgt tcgccgtcgg
     1501 catcctcatc gccgtcatcg gtacggggca ggcgttcatc gtgacggccg ccgccttcgc
     1561 catctcggcg ttgctgctgc agcgcctgcg gctggcgccc gtcgagcgtc gcgggacgca
     1621 tgccgaacac gacggcggca tgctcgaggg agcgcgctac atcttccgga acaagctctt
     1681 cctcgccacc atcgggctct cgttcttcac ctcggtgttc ggctcctcct acatcgtgct
     1741 gctgccgacc ttcgccgacg acatcctgca cgtcggcgcg cgcgggtttg gtctcatgga
     1801 gaccgccgcc gggatgggcg cgctgctcgg gacgttcctc atcgtgcgcg tgcgctcgca
     1861 gcgccattac ggccccgtca tgctgctcgc ggcggcggcc tttgggatcc tcatcgccgg
     1921 ctttgcggcc agccgcacga tcggcgtctc gatggcgctg ctgttcgggg gcggactcgc
     1981 ctcggccgtc tacctcaacg tcggcatgac gacgctgcag gtgctcgtcc ccgacgaatt
     2041 gcgcggccgg gtcatgggcg tctggagcat gacctacttc ctcggctccg tgggcggcct
     2101 gccggccggc ctggtggcgg cgtggctcgg cgcgccggtg gcggtggcga gctggtagcc
     2161 ctcgggatcc tg
//
LOCUS       DAORPG010000351         2165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6191_length_2165_cov_3.018957, whole genome shotgun sequence.
ACCESSION   DAORPG010000351 DAORPG010000000
VERSION     DAORPG010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6191_length_2165_cov_3.018957"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..669
                     /gene="cobU"
                     /locus_tag="PLX85_07725"
     CDS             109..669
                     /gene="cobU"
                     /locus_tag="PLX85_07725"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HRC63092.1"
                     /translation="MGEVWFVTGGARSGKSRFAERLATRTGRPVVYIATLEPLDDEMV
                     HRIANHRASRPAAWRTVEAPRELEAALAGAPSDACVLIDCLSLWVTNLLLDGPPELPP
                     QAVSALADDLSARLEALLDLAASRSGPLIVVSNEVGAGLVPEHALGRAFRDLLGWANQ
                     RVAARAARAWLLVAGRPIALPPEEDE"
     gene            complement(748..1908)
                     /locus_tag="PLX85_07730"
     CDS             complement(748..1908)
                     /locus_tag="PLX85_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRC63093.1"
                     /translation="MKSWLQAAVLGAVVVVAMVAGGLIAAAIAGGSDSSSTTDNSASV
                     STSSDGSKSDSASSVSLTAIDDLPTLVDRVSASVVAISTVSGSGTRQASGLGTGVVID
                     TDGHILTNFHVIDGARQVTVEFKDGSVVPGTVSGSDPGNDLAVVKVNLSADKLQPAKL
                     GDSDKTRQGESVFAIGNPFSEKFSVTSGIISATGRDSTGNTSIGRPVRGMLQTDAAVN
                     PGNSGGPLFNAAGEVIGINSSIENPTGQRVFVGVGFAVASNTAKRFLPDMIAGKPITH
                     PQLGVSGATLNDVNAQQLGIDAKKGVYITSVTSGSAADRAGLRAASGTSTDGGLPPGG
                     DVVTKFDGKDVTTIEQLAQLVDQHKVGDQVKLTVIRGGSQMELTATLQEWTG"
BASE COUNT          290 a          764 c          762 g          349 t
ORIGIN      
        1 gcatcgagga agatctgccg catccacccg agaacgcgcg tgacccagtt gcgcatccgc
       61 tcgcgcagcc gcgccgcctt ctgcgcgcgc cccacgaggt ggcagcgcgt gggtgaggtg
      121 tggttcgtca ccgggggcgc ccgcagcgga aagagccgct ttgccgagcg cctggcgacg
      181 cggaccggcc ggcccgtcgt ctacatcgcg acgctcgagc cgctcgatga cgagatggtg
      241 caccgcatcg cgaatcatcg ggcgtcgcgt ccggccgcgt ggcgcaccgt cgaagcgccg
      301 cgcgagctgg aggcggccct cgccggagcg ccgtcggacg cctgtgtcct gatcgactgc
      361 ctctcgttgt gggtgaccaa cctgctgctg gacgggccgc ccgagctgcc gccacaggcg
      421 gtgtctgcgc tggccgatga cctctcggcg cgcctcgagg cgctgcttga cctggcggcg
      481 tcacgctcgg gcccgctgat cgtcgtcagc aacgaggtgg gggcggggct ggtgccggag
      541 cacgcgctgg gccgtgcgtt tcgggacctc ctcggctggg cgaaccagcg ggtagccgcg
      601 cgcgccgccc gggcatggct gcttgtggcc ggccgtccca tcgcgctgcc gcccgaggag
      661 gacgagtagg ccgcgcggcc tgccccggaa acagagcagg cccccgcacg cgggggccct
      721 gctctgaagt ggacaggctt aggagggtta gcctgtccac tcctggagag tggcggtcag
      781 ctccatctgc gagccgccac ggatgacggt gagtttcacc tggtcgccca ccttgtgctg
      841 gtcgaccagc tgtgcgagct gctcgatcgt cgtcacgtcc ttgccgtcga acttcgtcac
      901 tacgtcgccg ccgggcggaa gcccaccgtc ggtcgaggtg cccgaggccg cccgcaggcc
      961 ggcgcggtcg gccgcgctgc cgctcgtcac ggaggtgatg tagacgccct tcttcgcgtc
     1021 aatgccgagc tgctgcgcgt tgacgtcgtt gagggtcgcg cccgagacgc ccagctgcgg
     1081 atgcgtgatc ggcttgccgg cgatcatgtc gggcaggaag cgcttggcgg tgttcgaggc
     1141 gaccgcgaag ccgacgccga cgaagacgcg ctggcccgtc gggttctcga tcgagctgtt
     1201 gatgccgatc acctcgccag ccgcgttgaa caaggggccg cccgaattgc cggggttcac
     1261 ggcggcgtcc gtctgcagca tgccgcgcac aggccgcccg atgcttgtgt tgccggtcga
     1321 gtcgcgcccg gtcgcgctga tgatgccgga ggtgacgctg aacttctccg agaacggatt
     1381 gccgatcgcg aacaccgact cgccctggcg cgtcttgtcg gagtctccga gcttggccgg
     1441 ctgcagcttg tccgccgaga ggttcacctt cacgaccgcg aggtcgttcc ccggatccga
     1501 gccgctcacg gtgcccggca ccacgctgcc gtccttgaac tccaccgtga cctgtcgcgc
     1561 gccgtcgatc acgtggaagt tggtgaggat gtggccgtcc gtgtcgatga ccacgccggt
     1621 cccgaggccg ctcgcctgcc gcgtgcccga gccgctgacg gtgctgatcg cgacgaccga
     1681 ggcggagacg cggtccacga gggtgggcag gtcatcgatg gccgtcaggc tgaccgagct
     1741 ggcgctgtcc gacttcgatc cgtcgctgct cgtggagacg ctcgcgctat tgtcggtcgt
     1801 gctggaggag tcgctgccgc ccgcgatggc cgccgcgatc aggccgccgg cgaccatcgc
     1861 caccacgacc accgctccca gcaccgctgc ctgcagccag gacttcatcc ccgacctcct
     1921 gtgcgaactc gtcttcctcg atgttcgggc acagcggtca agcgtgcgtg gcacaaatgt
     1981 aaagagggtg caatcgctac gcggatgtca cgccctcgag aggtgcggcc acgcctaccc
     2041 gaggcgtggg ccgcagtaca gcgccgcgcc gccccggtac aggctcgcct cgagctcggc
     2101 gccgatgctc tccgccagcg ccatcgaggc ccgcccgagc acgaggtcgc gcagcccgcg
     2161 cttcg
//
LOCUS       DAORPG010000352         2158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6216_length_2158_cov_3.283405, whole genome shotgun sequence.
ACCESSION   DAORPG010000352 DAORPG010000000
VERSION     DAORPG010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6216_length_2158_cov_3.283405"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PLX85_07735"
     CDS             complement(<1..590)
                     /locus_tag="PLX85_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRC63094.1"
                     /translation="MPAHPLLAALTEAVLPQRCIACGHFGAALHSHCLAALPRAEEPR
                     CPVCWTPGPGGPCTRCLEEPPAFEALRARFRFEGDVRRALLEAKFRGKTALLAPLAVA
                     AADVLPAAWHIDAVVPVPLHRGRQRQRGYNQAALIAKTVARRLDCPLAAGLLRRSRAT
                     APQARLPAAERATNLRDAFEAHDAVGEVLLIDDVTTT"
     gene            634..1350
                     /locus_tag="PLX85_07740"
     CDS             634..1350
                     /locus_tag="PLX85_07740"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HRC63095.1"
                     /translation="MQVKVMLFAGLRELLGSELVEGFEGDEVSVSEFRLRLGERYPQL
                     GPHLECAAIAVNEEYVREGSAAIHAGDVVALIPPVSGGSGETPHFLVTADPLDRDALR
                     ELVRTDEAGAVVLFEGIVRNHHQGHAVLRLEYEAYPSMAEKQLAAVAREVLEEFGERE
                     VYDLAAHHRTGTIEIGEASLLVAVSAAHRRDAFEAALRAVDRIKETVPVWKKEYGPEG
                     AVWQEGVEPSPSATGTAQRL"
     gene            complement(1681..>2158)
                     /locus_tag="PLX85_07745"
     CDS             complement(1681..>2158)
                     /locus_tag="PLX85_07745"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRC63096.1"
                     /translation="RGEDWIEEDTPIDTEGRIGPVIAEMESLVEESPLEWFILRGGVF
                     VGPDTFQDTTVTRLRGGEERVPGSGEQWTSFIHVADYADAVAAALQSSASRAVLNVTA
                     EPIRYRDYLARLAESLEVAPPLPEPGAPTPRSYRCSNAAASALLGWTPSRPIWPVA"
BASE COUNT          302 a          630 c          896 g          330 t
ORIGIN      
        1 gtggtggtca cgtcatcgat tagcagcact tcaccaacgg cgtcgtgcgc ctcgaaggca
       61 tcccgcaggt tcgtggcgcg ctcggcagcg ggaagcctcg cctgcggcgc ggtggcgcga
      121 gagcggcgga gcaggccggc agcgagcggg cagtcgagac gcctcgcgac ggtcttcgcg
      181 atcagcgcgg cctggttgta gccgcgctgg cgctggcggc ctcgatggag cgggaccggg
      241 acgaccgcgt cgatgtgcca ggcagcgggc agcacatcag cggcggcgac ggcgaggggc
      301 gcgaggaggg ccgtcttccc ccggaacttg gcctccagga gggcgcggcg gacgtcgcct
      361 tcgaagcgga agcgggcccg gagggcttcg aatgccgggg gctcctcgag gcagcgtgtg
      421 caggggccgc ccggcccggg cgtccagcag acggggcagc ggggctcctc ggcacgagga
      481 agcgcggcaa ggcaatgcga atggagggct gcgccgaagt gtccgcacgc gatgcagcgc
      541 tgcgggagga cggcctcggt gagggcggcg aggagcgggt gagcgggcat gggcgccccc
      601 gatgcggggc tatactagcg gaaaggagcg gcgatgcagg tcaaggtgat gctgttcgcg
      661 ggactgcgcg aactcctcgg cagtgagctg gtggaggggt tcgaaggcga cgaggtgtcg
      721 gtgtcggagt tccgtctgcg cctcggtgag cggtatccgc agctggggcc gcacctggaa
      781 tgcgcggcga tcgcggtgaa cgaggagtac gtgcgcgagg ggtcggccgc gatccacgcg
      841 ggcgacgtcg tcgcgctcat cccgcccgtc tcgggcgggt cgggggagac gccgcatttc
      901 ctcgtgacgg ccgacccgct ggaccgcgac gcgctgcgcg agctggtgcg gacggacgag
      961 gcgggcgcgg tggtgctgtt cgagggcatc gtgcgtaacc accaccaggg gcacgcggtg
     1021 ctgcggctgg aatacgaggc atacccctcg atggcggaga agcagctcgc cgctgtggcg
     1081 cgcgaggtgc tggaggagtt tggggagcgc gaggtgtacg acctggcggc acatcaccgg
     1141 accgggacga tcgagatcgg tgaggcgtcg ctcctcgtgg cggtgtccgc ggcacaccgg
     1201 cgcgatgcgt tcgaggcggc gctacgggcg gtggaccgca tcaaggagac ggtgccggtc
     1261 tggaagaagg agtacgggcc ggagggggcg gtgtggcagg agggcgtgga gccctcgcct
     1321 tcggcgacgg ggacggcgca gcggctgtag gcgcggcggc tctcgcctcg atgtgatccg
     1381 gttgatggac ggtggttgcg ggcggcgcgg ggttgttcct cgggggcaca ctgtgcctcg
     1441 caggttcatt gctcggaagc gccaagctgg ttcctcgacg atgttggctc tgacctcgtg
     1501 aggcggttgt ccgggggcgg ccggcagcgc cggccgattt gggcggcccg cttcgcgggc
     1561 cggcgtcttt tttcggaagg gccaccctcc cgccctggaa agggggcttc gtcccttttg
     1621 aatccccctt gtcggagccg tagtgatgcg ggagttgcag gagtgacctc ggtggccgcc
     1681 ctacgcgacg ggccagatcg ggcggctggg ggtccagccg aggagtgcgc tggcggcggc
     1741 gttggagcag cgatacgacc tcggggttgg ggcgccgggt tcggggagcg gcggggcgac
     1801 ttcgagggat tcggcgaggc gggcgaggta gtcgcggtag cggatgggtt cagcggtgac
     1861 gttgaggacg gccctcgatg cgctcgactg gagcgcagcg gcgacggcgt cggcgtagtc
     1921 ggcgacatgg atgaaggagg tccattgctc gccggagccg ggcacgcgtt cttcgccccc
     1981 gcgcagtctc gtgacggtgg tgtcctggaa ggtgtcagga ccgacgaaga cgccacctcg
     2041 gaggatgaac cactcaaggg gcgactcttc cacgagtgat tccatctcgg cgatgaccgg
     2101 gccgatgcgg ccctccgtgt cgattggcgt gtcctcctcg atccagtctt cgcctcga
//
LOCUS       DAORPG010000353         2157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6223_length_2157_cov_3.511893, whole genome shotgun sequence.
ACCESSION   DAORPG010000353 DAORPG010000000
VERSION     DAORPG010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6223_length_2157_cov_3.511893"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PLX85_07750"
     CDS             <1..841
                     /locus_tag="PLX85_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC63097.1"
                     /translation="WLFARGKPVALAPSARSGVALSLLLLGANAGLWHAALKPPESNA
                     SVTVLDVGQGLAVLVRDGGHAVLIDAGPPDAAVVGALSRAGQFATLDALVLTHEDLDH
                     TGGATAVERRVGARQVLAASDASHPSTGIIDIGDRMHLTARTTIEVLSPPVLSDSAPR
                     SDNDRGLVLLVTIGERRVLLPADIEAAAEAWLVQSAVDLDADALVVPHHGSKTSSTPA
                     FLAAVHPQVAVVSVGAHNAYGHPNAEVLARYADARVLRTDEDGDVTLTSDGQRLWYHA
                     ARQ"
     gene            907..1644
                     /gene="rph"
                     /locus_tag="PLX85_07755"
     CDS             907..1644
                     /gene="rph"
                     /locus_tag="PLX85_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007021484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRC63098.1"
                     /translation="MRERALGRTSDALRDVRVEPGVLRHAEGSALIEVGDTRVLCAAS
                     VEPGVPGFLRGSGSGWVTAEYGMLPRSTHTRSAREAARGRQGGRTQEIQRLIGRSLRA
                     VVDLDRLGEQTITLDCDVLQADGGTRTASITGAWVALAMAIDWMQREGRLPTQPLRTQ
                     VAATSVGMIEGQPLLDLDYSEDSKAGVDFNVVMLGTGQLVEVQGTAEGEPFTRRQMDG
                     LIDLAEAGIRQLFTVQRDAVNEWKVAR"
     gene            complement(1648..1974)
                     /locus_tag="PLX85_07760"
     CDS             complement(1648..1974)
                     /locus_tag="PLX85_07760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRC63099.1"
                     /translation="MQRYCVFCEIIARREPAEILYESEDVIVIRNVLRWVPVMLLAMP
                     KRHMTQSELWQNVGEVGTIAAEMGRRFCPQGFRLASNFGWHAMQSQEHAHLHVLGGTY
                     LGEYVS"
BASE COUNT          332 a          777 c          720 g          328 t
ORIGIN      
        1 ctggctcttc gcgcgcggca agccggtcgc gctcgccccg tccgccaggt cgggggtcgc
       61 gctctcgttg ctgctcctcg gcgccaacgc cggtctctgg catgcagcgc tcaagccacc
      121 cgagtcgaac gccagcgtca ccgtcctcga tgtcggacag ggcctcgcgg tgctcgtgcg
      181 tgacggtggt cacgccgtgc tcatcgacgc gggaccccct gacgctgccg tcgtcggtgc
      241 gctgagtcgc gccggtcagt ttgcgaccct cgacgcgctc gtcctgacgc acgaggacct
      301 cgaccacacg ggcggagcca ctgctgtcga acggcgagta ggcgcccggc aggtgctggc
      361 ggcgtctgac gcgtcacatc cctccacggg catcatcgac atcggagacc ggatgcatct
      421 gaccgcgcgg accaccatcg aggtgctctc accacccgtg ctgtccgact ccgcaccgcg
      481 ctcggacaat gaccgcggcc tcgtgctcct cgtgaccatc ggcgagcgcc gtgtcctgct
      541 cccggcggac atcgaagccg ctgcggaggc gtggctcgtc caaagcgcgg tcgatctgga
      601 tgcggacgcc cttgtcgtcc cccaccacgg ttcgaagacc tcgtcgacgc ccgcgttcct
      661 cgcggcggtg caccctcagg tcgcggtcgt ctccgtcggt gcgcacaacg cctacggtca
      721 cccgaacgcg gaagtcctcg cgaggtacgc ggacgccagg gtgcttcgca ccgacgagga
      781 cggcgatgtg acgctgacga gcgatgggca gcgtctctgg taccacgccg ctcgtcagtg
      841 acgggcggcg ctgctacggt cggacacacc cacagacacg cggagcagag gagtcgaaca
      901 atgacgatgc gcgagcgcgc gcttggccga acctccgacg cccttcgcga cgtccgcgtc
      961 gaacccggcg tcctgcgcca cgccgaaggc tccgcgctca tcgaggtcgg cgatacccgc
     1021 gtgctctgcg ccgccagcgt ggagcccgga gtgcctggct tcctgcgcgg ctcggggtcc
     1081 ggctgggtca ctgcggagta cggcatgctg ccgcgctcca cgcacactcg ttcggctcgc
     1141 gaagctgcac gagggcggca gggcggccgc acgcaggaaa tccagcggct catcggccgc
     1201 tcgctccggg cggtggtcga cctcgaccgc ctgggcgagc agacgatcac cctcgactgc
     1261 gacgtgctgc aggcagacgg cggcacgcgc accgcctcga tcaccggcgc ctgggtcgcg
     1321 ctcgccatgg ccatcgactg gatgcagcgc gagggccgcc tgccgacgca gccgctccgc
     1381 acccaggtcg ccgcgacctc ggtcggcatg atcgaaggcc aaccgctgct cgacctcgac
     1441 tactcggaag actcgaaggc cggcgttgac ttcaacgtcg tgatgctcgg cacaggtcag
     1501 cttgtcgagg tgcagggcac cgccgagggc gagccgttca cgcgccgcca gatggatgga
     1561 ctgatcgacc ttgccgaggc ggggatccgc cagcttttca cggtccagcg cgatgccgtg
     1621 aacgagtgga aggtcgcgcg ctaggcgcta cgacacgtac tcgccgagat acgtcccgcc
     1681 gaggacgtgc aggtgcgcgt gctcctgcga ctgcatcgcg tgccacccga agttcgaggc
     1741 caggcggaac ccctgtgggc agaaccgccg gcccatttcg gcggcgatgg tgccgacctc
     1801 gcccacgttc tgccacagct cggactgcgt catgtgccgc ttcggcatcg cgaggagcat
     1861 caccggcacc caccgcagga cgttccggat tacgatcacg tcctcggact cgtacaggat
     1921 ctcggcgggc tcccggcgcg cgatgatctc gcagaacacg cagtagcgct gcatctactg
     1981 accgggtacc ttccgaggac tgtgggatcg taggcggcca gtctagggac gcctgaaaag
     2041 cacggtcaat cagagccccc aatacgcgcg gagcggaagg acgccgtccg tgaccgagat
     2101 ccggccgttc cgaggcctgc gctacgaccc ggcgatggcg ccgccctcgg ctgtcat
//
LOCUS       DAORPG010000354         2156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6226_length_2156_cov_3.594479, whole genome shotgun sequence.
ACCESSION   DAORPG010000354 DAORPG010000000
VERSION     DAORPG010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6226_length_2156_cov_3.594479"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..788)
                     /locus_tag="PLX85_07765"
     CDS             complement(165..788)
                     /locus_tag="PLX85_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRC63100.1"
                     /translation="MTVAAIILAAGASRRMGRPKGLLPWGDRSLLAWEVDELMRSSAD
                     DIVVVLGHHAEDVRRSLGDGARYCVFNQRWPQGRATSLSKGAKALVAGGRAAPEAIVI
                     QNVDQPTRADIIDRLVAELRSSGAEVVQPSYRGKAGHPVVLSGALIDELIGATEATFG
                     LRSVLERHAAHLVAMDDEPVVRIDLDTPDTLDEGRRLLGVAGVATNG"
     gene            complement(798..1184)
                     /locus_tag="PLX85_07770"
     CDS             complement(798..1184)
                     /locus_tag="PLX85_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HRC63101.1"
                     /translation="MAPEYYSPTLIDHFEHPRNAGAMEDADAEAYVVNPVCGDSLRLF
                     LKIEDDRVQRASFLTSGCPASIATSSAATEMLAGRSLEDAEALTRDDYAEAVGGLPKS
                     KVHCSVLAASAVRQAIGEWRKQRAAV"
     gene            1281..1613
                     /locus_tag="PLX85_07775"
     CDS             1281..1613
                     /locus_tag="PLX85_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRC63102.1"
                     /translation="MSPRPLHHDPFDALGDPHRRAIVELLGSGGRSVREIADTLPISR
                     PAVSRHLRLLKEAGLVSDRPEGTRRIYELDSEGVAAVQAYLERVWGDVAARFRLAAEN
                     TTPRERAG"
     gene            1610..2047
                     /locus_tag="PLX85_07780"
     CDS             1610..2047
                     /locus_tag="PLX85_07780"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRC63103.1"
                     /translation="MTEPLRFTFELSCPPDHAFEVWTRRASLWWPKAHTISGESGLEV
                     VFEPHVGGRTFERTPAGLEHDWGEVTVWEPPHRLAYLWYIATDRASGTDVEIRFHPLD
                     DGRTRVEIEHAGWERLGERGPDWRNANVGGWEGVLPHYLEAAR"
BASE COUNT          324 a          778 c          751 g          303 t
ORIGIN      
        1 cagcagcatg aggtcgatct cctcggacgg cacgccgagc tggaacaggt gcggcagcgt
       61 ctgcggcggg cagtcgagga ggatgtgccg gtcgaggagg aaaccgctcc agtaccgccc
      121 gaacgaaaac gcgttgccgg accccaggca gtcgagccgc aagctcagcc gttcgtcgcc
      181 acgcccgcga ccccgagcag ccgccgcccc tcgtcgaggg tgtccggcgt gtcgaggtcg
      241 atgcgcacca ccggctcgtc atccatcgcg acgaggtgcg cggcgtgccg ttcgaggacg
      301 gaacgcaggc cgaaggtcgc ctcggtggcc ccgatcagct cgtcgatcaa cgcgccgctg
      361 aggacgaccg gatgcccggc cttcccgcgg tacgagggct gcaccacctc ggcgccgctg
      421 ctgcgcagct cggcgacgag gcggtcgatg atgtcggcgc gcgtcggctg gtcgacgttc
      481 tggatcacga tcgcctcggg cgcggcgcgg ccgccggcga cgagcgcctt cgcgcccttc
      541 gacagggacg tcgcgcggcc ctgcggccag cgctggttga agacgcaata ccgcgcgccg
      601 tcgccaagcg agcgccgcac gtcctcggcg tgatgtccga ggaccaccac gatgtcgtcc
      661 gccgaggagc gcatcagctc atcgacttcc caggcgagca gcgaacggtc gccccagggc
      721 aacagcccct tcgggcggcc catacgcctc gaagcgccgg cggcgaggat gattgcggcg
      781 accgtcatgc tcggagacta aaccgccgcc cgctgcttgc gccactcgcc gatcgcctgg
      841 cgcaccgccg aggcggcgag caccgagcag tgcaccttcg atttcggcag cccgccgacc
      901 gcctccgcgt agtcgtcgcg ggtcagcgcc tcggcgtcct cgaggctccg gcccgccagc
      961 atctcggtcg ccgccgagga ggtggcgatc gaggcgggac agcccgaggt caggaacgag
     1021 gcgcgctgga cgcggtcgtc ctcaatcttg aggaacaggc gcagcgagtc gccgcagacg
     1081 ggattcacga cgtacgcctc ggcgtccgcg tcctccatcg cgccagcgtt gcgaggatgt
     1141 tcgaaatggt cgatcagggt cgggctgtag tactcgggag ccatggctca atggtacgcc
     1201 cgagggcgcg cctccgccgc ctgcatgaac acgaagtgta gccgagacgt tacggttcgc
     1261 accctgttac catcgcgggc gtgtccccgc gtcccctcca ccatgacccc ttcgatgctc
     1321 tcggcgaccc gcatcggcgc gccatcgtcg aactcctcgg tagcggtggg cgctccgtcc
     1381 gcgagatcgc ggacacgctt ccgatcagcc gcccggccgt ctcacggcac ctccggctgc
     1441 tcaaggaggc cggtctcgtg agcgaccgcc ccgagggcac ccgccgcatt tacgaactcg
     1501 actcggaagg cgtcgccgcc gtgcaggcct acctcgagcg cgtgtggggc gacgttgcgg
     1561 cgcgctttcg cctggctgcc gagaacacca cgccccgcga gcgagcgggg tgaccgagcc
     1621 gcttcggttc accttcgaac tgagctgtcc gccggatcac gccttcgagg tgtggacgcg
     1681 gcgcgcctcg ctgtggtggc cgaaggcgca cacgatctcc ggcgagagcg gcctcgaggt
     1741 cgtcttcgag ccacacgtcg gcgggcgcac cttcgagcgc accccggcgg gcctcgagca
     1801 cgactggggc gaggtgacgg tttgggaacc gccgcatcgc ctcgcctacc tgtggtacat
     1861 cgcgacggac cgcgcgagcg ggaccgacgt cgagattcgc ttccaccccc tcgatgacgg
     1921 ccgcacccgc gtcgagatcg agcacgcggg ctgggagcgc ctcggtgaac gcgggccaga
     1981 ctggcggaac gccaatgtcg gcggctggga gggcgtgctg ccgcactacc tcgaggcggc
     2041 ccggtagccc tccgggctgc gcaggcacgc cagcccctcg aggggcgcgc ccttcgccat
     2101 tgccagcacg cggatgcggc cgaagggatt gccgaggatg agccgcttgg ccacct
//
LOCUS       DAORPG010000355         2144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6293_length_2144_cov_3.620871, whole genome shotgun sequence.
ACCESSION   DAORPG010000355 DAORPG010000000
VERSION     DAORPG010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6293_length_2144_cov_3.620871"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PLX85_07785"
     CDS             <1..511
                     /locus_tag="PLX85_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HRC63104.1"
                     /translation="AAEYASSLSSLPALNSSEVDAAAAEALAPFDGNGKENPYTIHRE
                     LQDTMQSLVGIIRNEGELQQARARLEEFRERAKNVTVEGNRQYNPGWHLALDLRSMLT
                     VADLITRGAIERKESRGGHTREDYPNPDAHFGTVNIVQSLKDGELTWRQEPLPEMPAD
                     LKELFEEDH"
     gene            511..1275
                     /locus_tag="PLX85_07790"
     CDS             511..1275
                     /locus_tag="PLX85_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005440110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HRC63105.1"
                     /translation="MPVAHLRIWRGDDEDGDYREYDVEYGEGMVVLDVVHILQATQAP
                     DLAVRWNCKAGKCGSCSAEVNGKPRLMCMTRMQTFTPGETISIAPLKTFPLIRDLVTD
                     VGHNFNVARDIMPELKPRQPEADGTHRMHQIDIDRIQEFHKCIECFLCQDVCHVFREH
                     PENLDNFVGPRFSIQLAQVAMHPLDTNPKRDMAKAEFGLDYCNITKCCTEVCPEGIHI
                     TDNGIIPMKERAVDVYFDPLRFIGRKLFGGKEKNQA"
     gene            1326..>2144
                     /locus_tag="PLX85_07795"
     CDS             1326..>2144
                     /locus_tag="PLX85_07795"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRC63106.1"
                     /translation="MALAAASSAPPALRRSDVTDALLTEALRATAPECLVVFGAGGGV
                     GDVVEASGARRVVVVDRNVVHVRAAIDGLEGRSYVEVIASDGTSHLDSGLRADAVAIR
                     APREKQVALQLIHDAYRLLPSGGRLYLAGALRDGMKPYIEFASGLFGGMQPLAMRKGC
                     RVVLGIRPEASRALADPLFDHAHFHRLEADVRGMAIEVRSRPGVFAWDRLDEGTRALA
                     EAIEVAPGDRVLDLGCGNGVIGVIAGRLGAGAVTMVDADVVAVESARRTVTANGI"
BASE COUNT          355 a          767 c          701 g          321 t
ORIGIN      
        1 cgccgccgag tacgccagct cgctgtcctc gctgcctgcg ctcaactcct cggaggtcga
       61 cgccgcggct gccgaggccc tcgcgccctt tgacggcaac ggcaaggaga acccctacac
      121 catccatcgc gagctgcagg acacgatgca gtccctggtc ggcatcatcc gcaacgaggg
      181 cgagctgcag caggcgcgcg cgcgcctcga ggagttccgc gagcgcgcga agaacgtcac
      241 cgtcgagggc aaccgccagt acaacccggg ctggcacctc gcgctcgacc tgcgctcaat
      301 gctcacggtc gccgacctca tcacgcgcgg cgccatcgag cgcaaggaga gccgcggcgg
      361 ccacacccgc gaggactacc cgaacccgga cgcccacttc gggaccgtga acatcgtcca
      421 gtcgctgaag gacggcgagc tgacctggcg tcaggaaccg ctgcccgaga tgcccgccga
      481 cctcaaggag ctgttcgagg aggaccactg atgccggttg ctcaccttcg catctggcgc
      541 ggcgacgacg aggacggcga ctaccgcgag tacgacgtcg agtacggcga gggcatggtc
      601 gtcctcgacg tggtgcacat cctgcaggcc actcaggcgc cggacctcgc ggtccgctgg
      661 aactgcaagg caggcaagtg cggctcctgc tccgccgagg tgaacggcaa gccccgcctc
      721 atgtgcatga cccgcatgca gacgttcacg cccggcgaga cgatctcgat cgcaccgttg
      781 aagaccttcc cgctcatccg cgacctcgtc acggacgtgg ggcataactt caacgtggcc
      841 cgggacatca tgcccgagct caagccccgc cagcccgagg ccgatggcac gcaccggatg
      901 caccagatcg acatcgatcg catccaggag ttccacaagt gcatcgagtg cttcctctgc
      961 caggatgtct gtcacgtctt ccgtgagcac cccgaaaacc tcgacaactt cgtcgggccg
     1021 cgcttcagca tccagctcgc gcaggtggcg atgcatccgc tcgacaccaa cccgaagcgc
     1081 gacatggcga aggccgagtt cggcctcgac tactgcaaca tcacgaagtg ctgcaccgag
     1141 gtctgccccg agggcatcca catcactgac aacggcatca ttccgatgaa ggaacgcgcg
     1201 gtcgacgtct acttcgaccc gctgcgcttc atcggccgca agctgttcgg cggcaaggag
     1261 aagaaccagg cctgacgccc ccgggggcgg cggaagccgc cctccgctcg gcgtagactg
     1321 gacgcatggc cctcgctgca gcatcgtccg cgccgcccgc gctccgtcgt tccgatgtca
     1381 ccgacgccct gctgactgag gcgttgcgtg ccactgcgcc cgagtgcctc gtggtcttcg
     1441 gggcgggcgg cggcgtcggg gatgtggtgg aggcctcggg ggcgcgacgt gttgtggtcg
     1501 tggatcgcaa cgtcgtccac gtgcgcgccg ccatcgacgg cctcgagggc cgttcctacg
     1561 tcgaggtcat cgccagcgac ggcacgagcc acctcgattc cggattgcgc gccgacgccg
     1621 tcgcgatacg cgcgccccgc gagaagcagg tggcgctcca gctgatccac gacgcgtacc
     1681 ggctgctgcc gtccggaggc cggctgtatc tcgccggcgc actgcgcgac ggcatgaaac
     1741 cgtacatcga gttcgcgagc gggctcttcg gaggcatgca gcccctcgcc atgcggaagg
     1801 gctgccgcgt cgtcctcggt atccgtcccg aggcctcacg agcgctcgcg gacccgctgt
     1861 tcgaccacgc gcatttccac cgcctcgagg ccgacgtgcg cggcatggcc atcgaggtcc
     1921 gctcgcggcc cggcgtcttc gcctgggatc gcctcgacga gggaacgcgg gcgcttgccg
     1981 aggccatcga ggtcgcgccg ggggatcgtg tcctcgacct cggctgcgga aacggcgtca
     2041 tcggtgtgat tgccggccgc ctcggcgccg gagcggtgac gatggttgat gcggacgtcg
     2101 tcgcggtcga gtcggcccga cggacggtca ccgcgaacgg catc
//
LOCUS       DAORPG010000356         2130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6358_length_2130_cov_3.201446, whole genome shotgun sequence.
ACCESSION   DAORPG010000356 DAORPG010000000
VERSION     DAORPG010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6358_length_2130_cov_3.201446"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLX85_07800"
     CDS             <1..320
                     /locus_tag="PLX85_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63107.1"
                     /translation="EIGGNPSVAKAAALTKLVLSKPGVEKIAVMMNVVSNTRADIMAR
                     GIIKGCIEAGRDPGETIAIFRIPGSWEDEGFKILRKYGVDFVDRSVSMHEAAARAVAK
                     MGA"
     gene            322..1218
                     /locus_tag="PLX85_07805"
     CDS             322..1218
                     /locus_tag="PLX85_07805"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRC63108.1"
                     /translation="MSILIDENSTFIIQGITGREAVTMTRELLNYGSKVVGGVTPGRK
                     GRDVHGVPVEDTVRAFTERMEVTGAIVAVPPAFAADAVMEAIEGGIKLIVIVTERIPR
                     KQVAEFIEYAGQEGARIIGPNCLGLISPGKSKMGGIGGAADATQRAFKPGSIGVMSRS
                     GGMTVEISNALSEAGLGISTAASIGGDAIIGSNYAELMPLFEADPETKGIAIYSEPGG
                     PSEAMLAEYMTASKSRLPVVAFMAGRFMDAMPGMRFGHAGTIVEGRADTTAEKIARME
                     AAGISVAERIEDIPAILKQRIG"
     gene            1232..1444
                     /locus_tag="PLX85_07810"
     CDS             1232..1444
                     /locus_tag="PLX85_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63109.1"
                     /translation="MAMFIRVDIPEDVQAEEPLRKQLVEVCPVDIFALDGERVVTVEA
                     NLDECTLCDLCIEASGSRVKVVKLYE"
     gene            1572..>2130
                     /locus_tag="PLX85_07815"
     CDS             1572..>2130
                     /locus_tag="PLX85_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRC63110.1"
                     /translation="MSHLVLFDIDMTLLRLNAVGRRAMTAALRELVGIEDALEGIDFA
                     GRTDRWILRQALAGRHTPEDFERFLVELQAPYLRALEEQLPVLGGELLPGVTALLDAL
                     AGRGDVRLGVQTGNLREAAWFKLKAFDLDRYFIDGGFADDAEERDKLVATAIARLGGA
                     AEGHAVVVVGDSPYDVTAALANEAVA"
BASE COUNT          340 a          695 c          773 g          322 t
ORIGIN      
        1 gcgagatcgg cggcaacccc tcggtggcga aggcggcggc gctgacgaag ctcgtgctgt
       61 cgaagccggg cgtcgagaag atcgccgtga tgatgaacgt cgtctcgaac acgcgggccg
      121 acatcatggc gcgcggcatc atcaagggct gcatcgaggc cggccgcgac cccggcgaga
      181 cgatcgccat cttccgcatc cccgggtcct gggaggacga ggggttcaag atcctgcgca
      241 agtacggcgt cgacttcgtc gaccgctccg tctcgatgca cgaggcggcg gcgcgcgccg
      301 tcgcaaagat gggggcctga gttgtcgatc ctcatcgacg aaaacagcac gttcatcatc
      361 cagggcatca ccggccgcga ggccgtgacg atgacccgcg agctgctcaa ctacggctcg
      421 aaggtcgtcg gcggcgtgac gccgggccgc aagggccgag acgtccacgg cgtgcccgtc
      481 gaggacaccg tgcgcgcgtt caccgagcgc atggaggtca ccggcgcaat cgtcgccgtg
      541 ccgccggcgt tcgcggcgga cgccgtcatg gaggcgatcg agggcggcat caagctgatc
      601 gtgatcgtga ccgagcgcat tcctcggaag caggtcgcgg aattcatcga gtacgccggg
      661 caggaagggg cccggatcat cggcccgaac tgcctcgggt tgatctcgcc gggcaagtcg
      721 aagatgggcg gcatcggcgg cgccgccgac gcgacgcagc gcgccttcaa gccgggcagc
      781 atcggcgtca tgtcgcggtc cggcggcatg accgtcgaga tctcgaacgc actctccgag
      841 gcgggcctcg gcatctcgac cgccgcctcg atcggcggcg acgcgatcat cggatcgaac
      901 tacgccgagc tgatgccgct gtttgaggcg gacccggaga cgaagggcat cgccatctac
      961 agcgagcccg gcgggccgag cgaggcgatg ctggccgagt acatgaccgc ctcgaagtcg
     1021 cgcttgccgg tggtggcgtt catggccggc cgcttcatgg acgcgatgcc cgggatgcgc
     1081 ttcggccacg cgggcacgat cgtcgagggc cgcgccgata cgacggcgga gaagatcgcg
     1141 cgcatggagg cggcaggcat ctcggtagcc gagcggatcg aggacatccc cgcgatcctg
     1201 aagcagcgga tcgggtagga gtcagctcac gatggcgatg ttcatccgcg tcgatatccc
     1261 cgaggacgtg caggccgagg agcccctgcg caagcagctc gtcgaggtct gccccgtcga
     1321 catctttgcc ctcgacggtg agcgggtcgt gaccgtcgag gcgaacctcg atgagtgcac
     1381 gctgtgcgac ctctgcatcg aggcctccgg cagccgagtc aaggtggtca agctgtacga
     1441 atgaacgggc gagcgagggt ggcgcgacga tgacctcgtt tcgcgccgcc ctcgcgctgc
     1501 tcgccgcgct cgcctttgtc tcggcgtgtg gcggcaagag tcccgctccg gcgctgtttg
     1561 tgtagcgtcc cgtgtcacac ctcgtgctgt tcgacatcga catgacgttg ttgcggctca
     1621 acgctgtcgg gcggcgcgcc atgacggccg ccctacgcga gctcgtcggc atcgaggacg
     1681 ccctcgaggg catcgacttc gcggggcgca cggaccgctg gatcctgcgg caggcgctcg
     1741 ccgggcggca cacacccgag gacttcgaac gcttcctcgt cgagttgcag gcgccgtacc
     1801 tgcgggcgct cgaggagcag ctgccggtcc tcggcggtga actgctgccg ggcgtgaccg
     1861 cgctcctcga cgcgctggcc gggaggggcg atgtgcggct gggcgtgcag accggaaacc
     1921 tccgcgaggc ggcctggttc aagctgaagg cgttcgacct cgatcgctac ttcatcgacg
     1981 gtggcttcgc cgacgacgcc gaagagcgcg acaagctcgt agcgacggcg atcgcacgcc
     2041 tcggcggcgc cgccgagggc cacgcggtcg tggtggtcgg cgactcgccg tacgacgtga
     2101 cggcggcgct ggcgaacgag gctgtggcgg
//
LOCUS       DAORPG010000357         2129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6362_length_2129_cov_3.508197, whole genome shotgun sequence.
ACCESSION   DAORPG010000357 DAORPG010000000
VERSION     DAORPG010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6362_length_2129_cov_3.508197"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PLX85_07820"
     CDS             complement(<1..771)
                     /locus_tag="PLX85_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63111.1"
                     /translation="MRRPFRHVGVLMIVGIAALGLIAAAYTLWYEDLSLTANVSTAQF
                     DVDWSCQTGVGTAPDGAGTNTTNNQTCTAATRDTVAILTTAATTTNPTLAWADFTDQG
                     DSPSTVPDTKYSQTCSNPVIGNNAAGANGNTGNNNTLTLTMTGLYPFAGCKFALDIDV
                     PTGNYVPAHFSLTAESNNSNGAVKVLAPTATGACAQIAAALSQTSATVPAVITDGTNP
                     IQLHPGEHLFCTFLIYLQELNASSATVAENTSFTITATI"
     gene            complement(978..>2129)
                     /locus_tag="PLX85_07825"
     CDS             complement(978..>2129)
                     /locus_tag="PLX85_07825"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRC63112.1"
                     /translation="ERRANLRLLIRMTPPVRKRAGAQALDPTTVDALPDQIAVIDRDG
                     VIVWVNEAWTDFARSAGASDEVCQGVGTHYLDECRRVAAQGSAEAAQSLAAIESVLNG
                     EQARASARYSCRTSEWRRSFELTASGLGLGNGAILVHHDVSATDDAEEHGGVASDAAA
                     ASTGWPVGGAAELPSFGRHDNALLDAIPDLMFEVDSDARILSFRPARDFDPYLPPAEF
                     LGRRVTDVLPSPVGDRLADAIAAAFDTGVIQVVAYQLEVADEARDYEARVIRATESVA
                     FGIVRDYTTQRREQLETDRRREVEELEGLVERQLLRRNPYHLTFRELTVLNLVAAGLA
                     DKAIAERLVLSTYTVNKHVAHILAKMGVQSRTQAAMQAQRDGLLAVDPD"
BASE COUNT          348 a          642 c          733 g          406 t
ORIGIN      
        1 gatcgtcgcg gtgatcgtga acgaggtgtt ctcggccacg gtcgcgctcg atgcattcag
       61 ttcctgcagg tagatcagga acgtgcagaa caggtgctcg ccgggatgca gctgaatggg
      121 atttgtccca tccgtaatga cggccggcac ggtcgcggag gtctgactca gtgccgccgc
      181 gatttgagcg caggctccgg tcgccgtcgg tgcgagcact ttgacggcgc cattgctgtt
      241 gtttgactca gccgtgaggg agaaatgcgc cgggacgtag ttgccggtgg gcacatcgat
      301 atccagagcg aatttgcagc cggcgaacgg atacagacca gtcatcgtca gcgtcagcgt
      361 gttgttgttg cccgtattgc cgttggcacc ggcagcattg ttaccgatga cgggattgga
      421 gcaggtctgg ctgtacttag tgtcgggcac cgtggacggg ctgtcgccct gatccgtgaa
      481 gtccgcccag gccagcgttg ggttggtcgt cgtcgcggcc gtggtgagga tggcgacggt
      541 gtcgcgggtg gccgcggtac acgtctggtt gttggtcgtg ttcgtgcccg cgccatcagg
      601 cgctgtgccg acgccggtct ggcatgacca gtcgacgtcg aactgggccg tcgagacgtt
      661 cgcggtgagg ctcagatcct cgtaccacag tgtgtaggcc gcggcgatga ggccgagggc
      721 ggcgatcccc acgatcatca gtacgccgac gtgccggaac ggtcgtcgca tttgtacacc
      781 tccgagcgcg cattgaggtc aagaccaacg acacggaacg tccgtccgta cgcgtctgct
      841 gagacggcag accgtgggat tggacattca gaaccagtag aggtttgcgc tcggcctcag
      901 acaacgcgtg gcgggggttc catgcctaga actcggtagg aaactaccgg ttcttagcta
      961 ctcggatgct cgcgatctca gtccggatcg accgccagca gcccgtctcg ctgcgcctgc
     1021 attgccgcct gggtgcgtga ttgaacgccc attttggcca gaatgtgcgc cacatgcttg
     1081 ttcaccgtgt acgtgcttaa cacgaggcgt tcggcaattg ctttatcggc gaggccagcc
     1141 gcgacgaggt tgagcacggt caactcgcga aacgtcagat ggtacggatt gcgtcgcagg
     1201 agctggcgct ccacgaggcc ttcgagctcc tcgacctcgc ggcggcgatc agtctccagc
     1261 tgctctcggc gctgcgtcgt gtagtcgcgg acgatgccaa aggcgacgga ctcggtcgcg
     1321 cgaattacgc gggcttcgta gtcacgcgct tcgtcggcga cttcgagctg gtatgcgacg
     1381 acctgaatta cgccagtgtc gaacgccgcg gcgatggcgt cggccagccg gtcgccgacc
     1441 ggggagggca ggacatcagt cactcgacgt ccgaggaact ccgccggagg caggtacggg
     1501 tcgaagtcgc gcgcgggccg gaagctgagg attcgcgcgt cgctgtcgac ttcgaacatc
     1561 aggtccggaa tcgcatcgag cagcgcgttg tcgtggcgtc cgaacgacgg cagctccgct
     1621 gcgcccccga cgggccagcc cgtcgatgcg gctgctgcgt ccgaggcaac cccgccgtgc
     1681 tcctcggcgt cgtcggtagc gctcacgtcg tgatgcacga ggatcgcgcc gtttccgagg
     1741 ccgaggccgg aagcggtcaa ctcgaacgac cggcgccact cggatgtgcg gcaggagtag
     1801 cgagcagagg cccgcgcttg ttcaccgttc agtacggatt cgatggcggc cagggattga
     1861 gctgcttcgg cgctcccctg ggccgccacc cggcggcact cgtcgaggta gtgtgtcccc
     1921 acaccctggc agacctcgtc ggaagcgccg gcgctacggg cgaagtcagt ccaggcctcg
     1981 ttcacccaca cgatcacgcc atcgcggtcg atgaccgcga tctggtcggg aagggcgtcg
     2041 acagtcgtcg ggtcgagtgc ctgggcgccg gctctctttc gaacgggtgg cgtcatgcgg
     2101 atgagcagtc tcaggttggc gcgtctctc
//
LOCUS       DAORPG010000358         2128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6367_length_2128_cov_3.623734, whole genome shotgun sequence.
ACCESSION   DAORPG010000358 DAORPG010000000
VERSION     DAORPG010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6367_length_2128_cov_3.623734"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..754)
                     /locus_tag="PLX85_07830"
     CDS             complement(23..754)
                     /locus_tag="PLX85_07830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HRC63113.1"
                     /translation="MATPEELFEGDADRAGYVRGMFGDISARYDLMNTLMTGGRHHAW
                     RKAAAGALVRPGDAVLDVGCGTGDLSFACLNAGAGYVFGLDFARPMVVRARQKAVERR
                     AGIVSFGVGDATRLPVGDETFDVWCSAFVVRNIPDLDAALSEAWRVLKPGGRVGILEI
                     PRMERGLLRPGARVHFQHVIPRVGRLVSGHSSAYRYLPVSVDHFLTPDELSARLRMAG
                     FEVRRVRTFMFGTVALHVAEKPDDS"
     gene            864..1844
                     /locus_tag="PLX85_07835"
     CDS             864..1844
                     /locus_tag="PLX85_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63114.1"
                     /translation="MALRLLPWSPEYGTGMQFDVDASAAMNGEGSPTIEFTERRPWER
                     VVPHAEPPAAVQIVDGVRRAEAHAMDEAADGSLVLGLFGSYAVGAVCCNGRAQLLEDH
                     FRVERRYFQTAGDPISREIPGGNASLCFRAELPENATTANDLVAALNRKMLDEEEKLA
                     EELSRDESILTFVDGPLRLRSPGDHVIGYIKRVHRWYLPAEHQRLLLDLAVGERTPVF
                     RILEGAEGRHDRLSWFLRLADLRGHYHPLAGVMRLEVPGSLPCDAAAHLADQSALVLP
                     GLASSPVRDPRSPQNLTPVGALEAALTHRLGDRRWINRLLTAAIEREAAA"
     gene            1841..>2128
                     /locus_tag="PLX85_07840"
     CDS             1841..>2128
                     /locus_tag="PLX85_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013019252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC63115.1"
                     /translation="MTAFEEARPVGLVLGTRAEAATPLEFWVYVDEDSYLQLDDVVHV
                     RSPLPGGRPVEAIDLYGVVDEVRALQEGVSFDSDVALATRGVLPAQTAVAAH"
BASE COUNT          309 a          873 c          636 g          310 t
ORIGIN      
        1 cgtgcaaaga gacttgcgag gtttacgagt cgtccggctt ctccgcgacg tgcagcgcca
       61 ccgtcccgaa catgaacgtc cgcacccgcc gcacctcgaa ccccgccatg cgcagccgcg
      121 ccgacagctc gtccggcgtg aggaagtggt cgaccgacac cggcaggtag cggtacgcgc
      181 tcgagtgccc cgagacgagc cgccccacgc gcggaatgac gtgctggaag tggacgcgcg
      241 cccccggccg caagagcccg cgctccatcc gcggaatctc gaggatgccc acgcgcccgc
      301 ccggcttgag gacgcgccac gcctcggaga gtgcggcgtc gaggtcgggg atgttccgca
      361 cgacgaacgc cgagcaccac acgtcgaacg tctcgtcgcc caccggcagc cgcgtcgcgt
      421 cgccgacccc gaacgagacg atgcccgcgc gccgctcgac cgccttctgc cgcgcccgca
      481 cgaccatcgg ccgtgcgaag tccagcccga acacgtatcc cgcccccgcg ttgaggcacg
      541 cgaacgagag gtcccccgtc ccacaaccca catcgaggac cgcgtcgccc ggacgtacga
      601 gcgcccccgc cgccgccttc cgccaggcgt ggtgccgtcc gcccgtcatc agcgtgttca
      661 tcaggtcgta ccgcgccgag atgtcgccga acatcccgcg cacatacccc gcgcggtccg
      721 cgtccccctc gaacagctcc tctggcgttg ccatacgagg agcatagcgc cgcgctcact
      781 ggcggcatcg agtcaatagc ttaagcattt gagacgaaca gccgttctgc gctatactcc
      841 gcctcggctc cccggaggct ccaatggcgc tccgcctgct cccctggtcc cccgagtacg
      901 gcaccggtat gcagttcgat gtcgacgcca gcgccgccat gaacggcgag ggctcgccca
      961 cgatcgagtt caccgagcgc cgcccctggg agcgtgttgt ccctcacgcc gagccgccgg
     1021 ccgccgtcca gatcgtcgat ggcgtccgcc gcgccgaggc gcatgcgatg gacgaagccg
     1081 ccgacggctc gctcgtgctc ggcctgttcg gctcctacgc cgtgggcgcc gtctgctgca
     1141 acggccgcgc gcagctcctc gaagaccact ttcgagtcga gcgccgctac ttccagacgg
     1201 ctggcgaccc catctcgagg gagatccctg gcggcaacgc ctcgctctgc ttccgtgccg
     1261 agctgcccga gaatgccacc accgccaacg acctcgttgc cgccctcaac cgcaagatgc
     1321 tcgacgagga ggagaagctt gccgaggagc tgtcgcgcga cgagtcgatc ctgaccttcg
     1381 tggacggccc tctgcgcctc cgctcaccgg gcgaccacgt tatcggctac atcaagcgcg
     1441 tccaccgctg gtacctcccg gccgagcatc agcgcctcct gctcgacctc gccgtcggcg
     1501 agcgcacccc cgtgttccgc atcctcgagg gggccgaggg ccgccacgac cgcctctcct
     1561 ggttcctccg cctcgccgac ctgcgtggcc actaccatcc gctcgccggc gttatgcgcc
     1621 tcgaggtgcc cggatcgctt ccctgcgacg ccgccgctca cctcgccgat cagtccgcgc
     1681 tcgtgctgcc cggcctcgcc tcctcgcccg tgcgcgaccc gcgctcacca cagaacctca
     1741 ccccggtcgg cgcgctcgag gccgccctca cccaccgcct cggcgaccgc cgctggatca
     1801 accgcctcct caccgcagcc attgagcgcg aggccgccgc atgaccgcct tcgaggaggc
     1861 tcgccccgtc ggtctcgtcc tcggcacccg cgccgaggcc gctaccccgc tcgagttctg
     1921 ggtctacgtc gacgaggact cctacctcca gctcgatgac gtcgtccacg tccgctcgcc
     1981 gctccctggc ggccgccccg tcgaggccat cgacctctat ggcgtcgtcg acgaggtgcg
     2041 cgcgctccag gaaggcgtga gcttcgactc cgatgtcgcc ctcgccacgc gaggggtcct
     2101 ccccgcccag accgcggtcg ccgcccac
//
LOCUS       DAORPG010000359         2116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6424_length_2116_cov_3.582242, whole genome shotgun sequence.
ACCESSION   DAORPG010000359 DAORPG010000000
VERSION     DAORPG010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6424_length_2116_cov_3.582242"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..444)
                     /locus_tag="PLX85_07845"
     CDS             complement(124..444)
                     /locus_tag="PLX85_07845"
                     /inference="COORDINATES: protein motif:HMM:NF015004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HRC63116.1"
                     /translation="MRIWDVPCVQLSRSHLLGEHRELHALWNILRRLEAGESRVGYAR
                     HPETQRWVGHGEALWRRHEEQAAEIARRGWRHLSPLEGGVDGSDEWPAVRLPGSVPRY
                     LRED"
     gene            complement(444..950)
                     /locus_tag="PLX85_07850"
     CDS             complement(444..950)
                     /locus_tag="PLX85_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRC63117.1"
                     /translation="MTLQRFEATQLISAPLDEAWAFFSDPRNLSAITPPDMGFEITSE
                     VPDHIYPGLFVTYRVRPLLGVPVEWVTEITQVDEGSRFIDEQRMGPYRLWHHEHTFRP
                     AGDMTEMRDVVYYALPFGRLGALALPVVRSRIERIFEYRADVLDERFNHRYRSRPSVA
                     GTPTAAYV"
     gene            complement(947..1879)
                     /locus_tag="PLX85_07855"
     CDS             complement(947..1879)
                     /locus_tag="PLX85_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017591056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HRC63118.1"
                     /translation="MRIAITGATGYIGSALTEWLQSRGHEVRKITRGPSSDPAALWDP
                     ASRWARPGAFDDLDAVVHLSGASIAGKRWTAGRRALLRASRIDTTAFLAEHLAGLSRP
                     PRTLVTISGIGYYGDAGDALLSERSFRGSGFLADLVADWEAAAAPAREAGIRVVHART
                     APLIGPDAELLRRVLLPFRLGLGGRLGSGKQWFSWVSTHDLVRAIEFMLATPELAGPV
                     NLAAPGAVRNSEFTKALGRQLHRPTLFPIPAFALRLLFGKDLASETLLVSQRAVPGAL
                     EAGGFVFDLPTIEQALSTALAKRPTSTLEQGLAR"
BASE COUNT          339 a          698 c          793 g          286 t
ORIGIN      
        1 gacgttgaag ttgtggaagt cgcgccccgc ggcgcccagg atgacgaagc ggcgcggctg
       61 gtacatcgag gccccctccc cgctcctcga ttgtactgag ggcgccagcg cctccggacg
      121 cgctcaatcc tcgcggaggt agcgcgggac cgaaccgggc aagcgcacgg ccggccattc
      181 gtctgagcca tcgacgccgc cttcgagggg ggagagatgt cgccagcctc gccgcgcgat
      241 ctcggcggcc tgctcctcgt ggcggcgcca gagcgcttcg ccatgcccga cccagcgttg
      301 cgtctccggg tgtcgtgcgt agccgacacg gctctcgccc gcctcgaggc ggcgcaggat
      361 attccagagc gcgtgcagct cgcggtgctc gcccaggagg tgcgagcgcg agagctgcac
      421 gcaggggacg tcccagatgc gcatcagacg taagcagccg tcggggtgcc cgcgaccgac
      481 gggcgagatc gataccggtg gttgaaacgc tcgtcgagca catcggcgcg gtattcgaag
      541 atgcgctcga tgcgcgagcg gacgaccggg agcgcgagcg cgcccaaacg gccaaacggg
      601 agcgcgtagt agacgacgtc tcgcatctcg gtcatgtcgc ccgccggccg gaacgtgtgc
      661 tcgtggtgcc agagccggta cggccccatg cgctgctcgt cgatgaagcg cgagccctca
      721 tcgacctggg tgatctcggt cacccactcc acgggaacgc cgagaagggg acgcacccga
      781 tacgtcacga acagacccgg gtagatgtgg tccggcacct ccgaggtgat ttcgaatccc
      841 atatccggcg gggtaatggc ggagaggttg cgcgggtcag agaagaaagc ccaggcctcg
      901 tcgagcggag ccgagatcag ctgggtggcc tcgaatcgct ggagggtcat cgggccaacc
      961 cctgctcgag cgtcgaggtc gggcgcttcg ccagcgcagt tgagagcgct tgctcgatcg
     1021 tcggcaggtc gaagacaaag ccgcctgcct cgagggcgcc cggcaccgcc cgctgactca
     1081 cgagcagcgt ctccgaggcg aggtccttgc cgaacaacag ccgaagcgcg aatgcgggaa
     1141 tcgggaacag cgtcggtcgg tggagctggc gcccgagtgc cttcgtgaac tcgctattcc
     1201 ggacggcgcc cggtgcggcg aggttgacgg ggcccgcgag ttcgggcgtg gcgagcatga
     1261 attcgatcgc ccgcaccagg tcgtgtgtgc tgacccagga gaaccactgc ttcccgctgc
     1321 caagccgacc accgagaccc aggcggaacg ggaggaggac ccggcgcagg agttcggcgt
     1381 ccggcccgat gagcggcgcg gtgcgtgcgt ggaccacgcg aatgccagcc tcgcgcgccg
     1441 gggccgcggc agcctcccag tccgcgacca ggtcggccag gaagcccgag ccgcggaagc
     1501 tgcgttcgct cagcagggca tcgcccgcgt cgccgtagta gccgatgccc gagatggtga
     1561 cgagggtgcg cgggggacgg gacagcccgg cgaggtgctc cgcgaggaag gcggtcgtgt
     1621 cgatgcggct ggcccggagc agggcgcgcc ggccggccgt ccaccgcttg ccggcgatcg
     1681 aggcaccgga gagatgcacc accgcgtcca ggtcatcgaa ggctcccggg cgcgcccagc
     1741 gcgacgcagg atcccacagg gcggccgggt ccgagctcgg ccccctggtg atcttgcgca
     1801 cctcgtggcc gcgggactgg agccactctg tgagagcgct gccgatgtac ccggtggcgc
     1861 ctgtgatcgc gatacgcatg gttggacctc gattctctgc agatgagatg ggcagcgccg
     1921 ccgccggaag gaggacggcg gcggcgctgt ggtggacgac tagcggatcc gcgtgctcgc
     1981 gaggcgtcgg gcggcgacgg ggagagcgag cgcgacgagc gccagggagc cgatccagag
     2041 cgcgctccca ctatcgtccg tggccgcgcc agcgttcccg gtgcgcggcg cctgaggcgt
     2101 ggcggcgggg gtggcc
//
LOCUS       DAORPG010000360         2114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6433_length_2114_cov_3.094220, whole genome shotgun sequence.
ACCESSION   DAORPG010000360 DAORPG010000000
VERSION     DAORPG010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6433_length_2114_cov_3.094220"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /gene="gcvT"
                     /locus_tag="PLX85_07860"
     CDS             complement(<1..846)
                     /gene="gcvT"
                     /locus_tag="PLX85_07860"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRC63119.1"
                     /translation="MADLNGTLDPPDGLRRLALHEQHVAAGARFAPFAGWEMPLQYRG
                     IVAEHEAVRADVGVFDVSHLGRVWVRGPQAGARLRSATTFDVTALEPGSAHYSLYCNE
                     HGGIEDDVFVYRVDDERWLVAHNAANAAADFERVRSVFGDAAEESTGATVMLAVQGPR
                     APALLTALVGEAYTLLRPHGCIEVPWGGSTALVARTGYTGEDGGEVVVPSEVAEKLWD
                     ACLDLGATPAGLGARDTLRLEAALPLHGNDIDSTTNPFEAGLGFAVTLDDGAPFLGRD
                     ALARLA"
     gene            940..1446
                     /locus_tag="PLX85_07865"
     CDS             940..1446
                     /locus_tag="PLX85_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HRC63120.1"
                     /translation="MPTYVALLRAVNVGGTGKLPMSDLRALCAAAGFESVQTYIASGN
                     VVFTSDRPAADVKGRLEAALADYAGKPVGVLLRTPTELAAVLAANPFGAAPPNRVLVT
                     FLDSPPAEDAVDFATGRRDEEIALGEREIYIHYGEGMGSSKLTVPAARQGTARNINTV
                     RKLVEMAS"
     gene            complement(1481..>2114)
                     /locus_tag="PLX85_07870"
     CDS             complement(1481..>2114)
                     /locus_tag="PLX85_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020637261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HRC63121.1"
                     /translation="AELTWGLILALARHIPEEHAAIFGGRWQTTVGMELRHKTLGIVG
                     LGSLGTQVAKVGQAFGMDVIAWSQNLTAEHASERGVTRVEKDELFARADIVSVHVVLS
                     DRTRGLIGAQELGLMQPTAYLVNTSRGPIVDERALIDALRSRQIAGAALDVYDVEPLP
                     ADHPFRGLDNLLMTPHIGYVTGDTYQLWYRDALEDIQAFAAGNPIRVVER"
BASE COUNT          339 a          767 c          706 g          302 t
ORIGIN      
        1 ggcgagcctc gcgagagcgt cccggccgag gaacggcgcg ccgtcatcga gagtcacggc
       61 gaagccaagg cccgcctcga agggattcgt cgtcgagtcg atgtcgttgc cgtgtagcgg
      121 cagcgccgcc tcgaggcgca gcgtgtcgcg agctccgagc ccggcaggcg tggcgccgag
      181 gtcgaggcag gcgtcccaca gcttctccgc cacctcggac ggcacgacga cctcgccgcc
      241 gtcctcgccg gtgtaccccg tgcgagcaac gagcgccgtc gagccgcccc acgggacctc
      301 gatgcagccg tgcgggcgca acaaggtgta tgcctcgccg accagtgcgg tgagcagcgc
      361 tggcgcgcgg gggccctgga cagccagcat gaccgtggcg cccgtgctct cctcggccgc
      421 gtcgccaaag acggaccgaa cccgctcgaa gtcggcggcc gcgttcgccg cgttgtgggc
      481 gaccagccag cgctcgtcgt cgacgcggta gacgaacacg tcgtcctcga tgccgccgtg
      541 ctcattgcag tacagcgagt agtgcgcgct cccgggctcg agcgcggtca catcgaaggt
      601 cgtcgcggag cggagccgcg cgccggcttg cggcccgcgg acccacacac gtccgaggtg
      661 agacacgtcg aatacgccga cgtccgctcg caccgcctcg tgctccgcga cgatgccgcg
      721 gtactgcagt ggcatctccc agccggcgaa cggcgcaaac cgcgcaccgg ccgccacatg
      781 ctgctcgtgc agcgcgagcc gccgcagccc gtccggggga tcgagggtgc cgttcaggtc
      841 cgccacgaac tcactgtatc gctcgccggc acccctcgag gctcgcctac gcacgatcga
      901 tccacgaaac accacaggcg tttcgtacac tgctcggtga tgcccacgta tgtagccctg
      961 ctccgcgccg taaacgtcgg cgggaccggc aagctcccga tgagcgacct gcgcgcgctc
     1021 tgcgcggcgg ccggcttcga gtcagtgcag acctacatcg cgagtggcaa cgtcgtcttc
     1081 acgagcgacc ggccagcggc ggacgtcaaa ggacgcctcg aggcggcgct cgcggactac
     1141 gccggcaagc ccgtcggcgt actcctcagg acacctacgg aactcgcggc cgtcctggcc
     1201 gcaaacccgt tcggcgctgc gccgccgaac cgcgtgctcg tcaccttcct cgactcgccg
     1261 ccggcggaag acgccgtcga tttcgcgacc ggtcggcgtg acgaggagat cgcgctgggc
     1321 gagcgcgaga tctacatcca ctacggcgag gggatgggca gctcgaagct cacggtaccc
     1381 gccgcgcggc agggcaccgc gaggaacatc aacaccgtcc gcaagctggt cgagatggcg
     1441 agctgaccgg gcggccacga ccgccattca gggccagcgg ctagcgctcg acgacccgga
     1501 tgggattgcc cgccgcgaac gcctgaatgt cctcgagcgc atcgcggtac cagagctggt
     1561 aggtgtcgcc cgtgacgtat ccgatgtgcg gcgtcatgag cagattgtcg agccctcgga
     1621 acgggtgatc cgcaggcagc ggctcgacgt cgtacacatc gagggccgcg cccgcgatct
     1681 ggcgcgatcg cagggcgtcg ataagggcgc gctcgtctac gatcggccct cgggatgtat
     1741 tcaccaggta cgcggtgggc tgcatcaacc ccagctcctg cgctccgatc agacctcgcg
     1801 tgcggtcgct gagcacgacg tgcacggaga cgatgtcggc ccgggcgaac agttcgtcct
     1861 tctcgacgcg cgtcacccct cgttcggacg cgtgctcggc cgtcaggttc tggctccagg
     1921 cgatgacgtc catcccgaac gcctggccca ccttcgccac ctgcgtgccg aggctgccga
     1981 ggccgacgat acccagcgtc ttgtgccgca actccatacc gaccgtcgtc tgccaccgcc
     2041 ctccgaagat cgcggcgtgc tcctcgggga tatgccgagc cagcgcaaga atgagccccc
     2101 aggtgagctc cgcc
//
LOCUS       DAORPG010000361         2113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6440_length_2113_cov_3.306122, whole genome shotgun sequence.
ACCESSION   DAORPG010000361 DAORPG010000000
VERSION     DAORPG010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6440_length_2113_cov_3.306122"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="PLX85_07875"
     CDS             complement(<1..797)
                     /locus_tag="PLX85_07875"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRC63122.1"
                     /translation="MTAKPRVLVAMSGGVDSAVAAALLHEQGYQVVGVTLRLYTEADD
                     TSYRSKRSCCGIEDVADARAAAQRIGIPHYVLNMEQEFERDVLGPFVQAYAEGRTPNP
                     CLACNQHVKFSTLLERAVAMGCDLLATGHYARIDRVEEAPGVERYRLLAATDEQKDQS
                     YVLYTLDQGALARTLFPVGELPKSEVRRIAGSLGLRVADKPDSADICFVPGGDYRDLL
                     RARGGIDAEHRVVGPRADAPPLAVEHTDQREREREQRRDHTDGERVA"
     gene            1067..1393
                     /gene="rsfS"
                     /locus_tag="PLX85_07880"
     CDS             1067..1393
                     /gene="rsfS"
                     /locus_tag="PLX85_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRC63123.1"
                     /translation="MAHHVVDLLVDRQASDVVLLDLTSLSTFTDYFVIATAGNERQLN
                     ALIETLDADASAAGRPFKQEGLPDGGWVLVDLGTVIVHLFSLEKRAYYNLEGLWQRAQ
                     EVVRVQ"
     gene            complement(1473..1922)
                     /gene="ndk"
                     /locus_tag="PLX85_07885"
     CDS             complement(1473..1922)
                     /gene="ndk"
                     /locus_tag="PLX85_07885"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRC63124.1"
                     /translation="MERTLILVKPDAMQRGLAGEILARFERRGLRMVGLRLLKVDRNM
                     AKRHYAEHVGKPFFEGLVAYITSAPIVAAVFEGTNAVAAARQLMGSTRPTEAAPGTIR
                     ADFGLEVGRNLVHGSDSVASAKREIDIFFEGQRLASWKRDVDPWIFE"
BASE COUNT          325 a          658 c          799 g          331 t
ORIGIN      
        1 tcggctacgc gctccccgtc ggtgtgatca cggcgctgtt cgcgttcccg ttcacgctgg
       61 tcggtgtgct ctacggcgag tggcggcgca tccgcacgtg gacctactac gcggtgctcg
      121 gcatcgatcc ctccgcgagc gcggaggagg tcacggtagt cgccgccggg gacgaagcag
      181 atgtcggcgc tgtccggctt gtcggcgacg cgcaggccga gcgagccggc gatgcggcgc
      241 acctcggact tgggcagttc gccgacgggg aacagcgtgc gcgccagggc gccctggtcg
      301 agcgtgtaga gcacgtagga ctggtccttc tgctcatccg tggccgcgag caggcggtag
      361 cgctcgacgc caggggcctc ctcgacacgg tcgatgcgcg cgtagtggcc ggtcgcgagg
      421 agatcgcagc ccatggcgac agcgcgctcg aggagcgtcg agaacttcac atgctgattg
      481 caggcgaggc aggggttggg ggtgcgcccc tcggcgtagg cctgcacgaa ggggcccagg
      541 acgtcgcgct caaactcctg ctccatgttg aggacgtagt gagggatgcc gatgcgctga
      601 gcggcggcgc gtgcgtcggc gacgtcctcg atgccgcagc aggagcgctt cgagcggtac
      661 gaggtgtcgt cggcctcggt gtacaggcgg agggtgacgc ccacgacctg gtaaccctgt
      721 tcgtggagca gggcggccgc aacggcggag tcgacgccgc cggacatggc gaccaggacg
      781 cgtggcttcg cggtcatcgt tccattgtag gcgctgcctc ggtgagggcg gccgctagcc
      841 gagcagccag ctcacgagca gcaagatgag taccgacacg agagcactgc caaggacggc
      901 ggcgacccac gcgccctggt tggagggcat cacccttcaa gcgtatgcct gctggcgagg
      961 aagcagacgc gccggcggtg gggccagagg agagccctga tatactgctc aaccgggccc
     1021 cctcgaaggg gccaacgttg aggaggccgc gctgcagggt tctgaactgg cccaccacgt
     1081 cgtcgacttg ctggtggacc gccaggcgtc ggacgtggtg ttgctcgacc tcacctcgct
     1141 ctccacgttc accgactact tcgtgatcgc caccgctggc aacgagcgac agttgaacgc
     1201 gctcatcgag acgctcgatg cggacgcgag cgccgcgggg cggccgttca agcaggaggg
     1261 cctgccggac ggcgggtggg tgctggtcga cctcgggacg gtgatcgtcc acctgttctc
     1321 gctcgagaag cgcgcgtact acaacctcga ggggttgtgg cagcgggcgc aggaggtcgt
     1381 gcgggttcag taggcccgag gtcacgcagt gcgaaatgcg aaggcccgcc gtgaggcggg
     1441 ccttcgttgt tactgaggcg gaagcttcga ggctattcga agatccaggg gtcgacgtcg
     1501 cgcttccagg aagcgaggcg ctggccctcg aagaagatgt cgatctcgcg ctttgcactc
     1561 gcgacgctgt cggagccatg gacgaggttg cgaccgacct cgaggccgaa gtcggcgcgg
     1621 atggtgccgg gggcggcctc ggtggggcgg gtggagccca tgagctggcg ggcggcggca
     1681 acggcgttgg tgccctcgaa gaccgcggcg acgatgggcg cgctcgtgat gtaggcgacc
     1741 aggccctcga agaagggctt gccgacgtgc tcggcgtagt ggcgcttcgc catgttgcgg
     1801 tcgaccttga gcaggcggag gccgaccatg cgcaggccgc ggcgctcgaa gcgcgcgagg
     1861 atttcgcccg cgaggccgcg ctgcatggcg tccggcttca cgaggatgag ggtccgttcc
     1921 acaaccaccc cttccgagga gtgaccggcc gctagcgccg gtccgcgcgt gcgccgatgg
     1981 acggcggccg caggtagatg acgtcgactg cggcgggatc ggtgtaggcc cggtgcagcc
     2041 gcgccaaccg cacgaggtcg gcggcgcttc gcacattttc gctggcctcg gcgtcagtgt
     2101 cgcccgatcg acc
//
LOCUS       DAORPG010000362         2112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6448_length_2112_cov_3.152649, whole genome shotgun sequence.
ACCESSION   DAORPG010000362 DAORPG010000000
VERSION     DAORPG010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6448_length_2112_cov_3.152649"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PLX85_07890"
     CDS             <1..553
                     /locus_tag="PLX85_07890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC63125.1"
                     /translation="ADTEPIAISQSISRIIAEAQRQQSGRVIRLESADWGLFAIAAES
                     YVHQVMLNYLTNAMRFSPADRPIEVSVQQAGENIEVRVLDSGIGLAEEDLEALFTPFY
                     RSSRVPEDMRGMGIGLSVTRRLIEALGGQVWARRREQGGAEFGFSLPSASDGEGALEL
                     SPIATDLGPPVGHEDSADVPEPS"
     gene            complement(550..810)
                     /locus_tag="PLX85_07895"
     CDS             complement(550..810)
                     /locus_tag="PLX85_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63126.1"
                     /translation="MRNADIRFEMMDRLGAYLQGREGLADLLGWEAELSLSAWIAKPL
                     RADLDRIALMGEEVESGLRDEREVREVARSVLDGLAARARAS"
     gene            824..1111
                     /locus_tag="PLX85_07900"
     CDS             824..1111
                     /locus_tag="PLX85_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63127.1"
                     /translation="MPSVRVPEAELTQLFDRLDLWHKADVGVLTPVEEPGARMPARLC
                     EGGQSFFVRLRNAAGLDVARIHYIACPDGNLLRWPSALLLGEVTLYRVAHD"
     gene            complement(1112..1561)
                     /locus_tag="PLX85_07905"
     CDS             complement(1112..1561)
                     /locus_tag="PLX85_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63128.1"
                     /translation="MPGEYEIDVVDDDGEEPSAEALAELAAVSEAETDSTSAVEAALE
                     ATRRELEEQRRLALTAVARYRDAALAAEPELPPELVSGQTVEEVDASLAAARSTVASI
                     RQRLSEGAESSMRGFPAGAPARLGGSTEGLSAAEKIARGLERRARAT"
     gene            1734..>2112
                     /locus_tag="PLX85_07910"
     CDS             1734..>2112
                     /locus_tag="PLX85_07910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC63129.1"
                     /translation="MATVAQGHVIVRRMADADLKQVRRIERAAYGASLPGTPFERELR
                     NGLAEYLVAVEHPEPTVTPAEEHRSLLDSFRRLFSHEPEPRVLGFLGVWYTIDQLHVV
                     TVAVDPAEQGRGIAQRLLLEAHRL"
BASE COUNT          319 a          834 c          627 g          332 t
ORIGIN      
        1 cgcggacacc gagcccatcg ccatctcaca gagcatcagc cgcatcatcg ccgaggcgca
       61 gcggcagcag tccggccgcg tgatccgcct cgagtcagct gactggggcc tcttcgccat
      121 cgccgccgag agctacgtgc accaggtgat gctcaactac ctcaccaacg ccatgcgctt
      181 cagcccggcc gaccgcccga tcgaggtctc cgtccagcag gcgggcgaga acatcgaagt
      241 gcgcgtcctc gacagcggca tcgggctcgc cgaggaagac ctcgaggcgc tctttacgcc
      301 cttctatcgc tcctcgcgcg tccccgagga tatgcgcgga atgggcatcg ggctctccgt
      361 gaccaggcgt ctcatcgagg ccctcggcgg gcaggtctgg gcccgtcgtc gcgagcaggg
      421 cggcgccgag ttcggcttct cgctcccctc cgcctccgat ggcgaaggag ccctcgaact
      481 cagcccgatc gccacagacc tcggcccgcc cgtcgggcat gaggactcag ccgatgtccc
      541 ggagccgagt taggacgccc gtgctctcgc ggccagcccg tcgagcacgc tgcgcgcaac
      601 ttcacgcact tcacgttcgt cgcgcagccc ggattccact tcctcgccca taagcgcgat
      661 gcggtcgagg tcggcgcgta acggtttcgc tatccaggcc gacaggctga gttccgcctc
      721 ccacccgagt aagtcggcga gtccctcgcg tccttggagg tatgccccca gacgatccat
      781 catctcgaaa cgaatatctg cgttgcgcat gaaggtgacc tcgatgccct cagtcagggt
      841 accagaggcc gagctcactc agctctttga tcgcctcgac ctctggcaca aggcggatgt
      901 aggcgtactg actccggtag aggagccagg tgctcggatg ccggcgcggc tctgtgaggg
      961 cggccagtcg ttcttcgtcc gactgcgaaa cgcagccggg ctggatgtgg cacgcatcca
     1021 ctacatcgcc tgccctgatg gcaaccttct gcgctggccg tccgcgctcc tccttggcga
     1081 ggtgacgctc taccgcgttg cccacgactg actaggtcgc gcgcgcacgc cgctcgaggc
     1141 ccctcgcgat cttctccgcc gccgacagcc cctcggtcga accgccgaga cgagcgggag
     1201 caccggccgg gaacccccgc atcgaggact cagccccctc agacagccgc tgccggatcg
     1261 aggcaacagt gctccgtgcc gcagccagcg acgcatccac ctcctcgacc gtctgcccgg
     1321 acaccagctc cggaggcagc tcgggctccg ccgccagagc cgcgtcccgg taacgggcca
     1381 ccgccgtcag agccagccgc cgctgctctt ccagctcccg ccgcgtcgcc tcgagcgccg
     1441 cctccacggc cgaggtcgaa tccgtttccg cctcgctcac cgccgccagc tccgccagag
     1501 cctccgcgct cggctcttca ccgtcgtcgt ccaccacgtc gatctcgtac tcacccggca
     1561 tccgaggcgc tcccttcgcc gccgccctcc gcgacggcgt ggccaccgta gccacgcgcg
     1621 ggccgcaact cggagcaccc tctggcacac caccgcaccc gccgagcaag cagcgccctc
     1681 tccgagcttc cccggcccgc cggcgcaacg atcggtcttg atacactggc gtaatggcga
     1741 ctgtcgcgca gggccacgtc atcgtccgcc gtatggcgga cgccgacctg aagcaggtgc
     1801 gccgcatcga gcgcgcggcc tatggcgcct cgctccccgg caccccgttc gagcgcgagt
     1861 tgcgcaatgg cctcgccgag tacctggtcg ccgtcgagca ccccgaaccg accgtcacgc
     1921 ccgccgagga gcatcgctcg ctcctcgact cgtttcgtcg cctcttcagc cacgaacccg
     1981 agccgcgcgt actcggcttc ctcggcgtct ggtacacgat cgaccagctc catgtcgtta
     2041 ccgtcgccgt cgaccccgcc gagcagggcc gcggcatcgc gcagcgcctg ctcctcgagg
     2101 cgcaccgtct cg
//
LOCUS       DAORPG010000363         2109 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6463_length_2109_cov_3.507790, whole genome shotgun sequence.
ACCESSION   DAORPG010000363 DAORPG010000000
VERSION     DAORPG010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6463_length_2109_cov_3.507790"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            383..1588
                     /locus_tag="PLX85_07915"
     CDS             383..1588
                     /locus_tag="PLX85_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63130.1"
                     /translation="MLVRLRGLHLVLATMIGMLALVVTPRLQHSGAATPALAACANAA
                     DVQLEADYIVASQYVKKNDRASGAINNVFGAPTWVVPRENAMAILGLVRASDCLGASS
                     YRQRAQQAADYLVRMQASDGSWYDQYSYAAPVVLSKSPTQTAEVMLALDRLGFSRSWY
                     AAMMGGADFLVRLQDPTNKGGADDGLLGGGLDSNGSYLRWRWTSDNSYGYQALKAAEK
                     WARTQGDAVRANRYAAASGRILNGINTRLKDPGSPVWYGAIDENGVSTLIEHEWINYA
                     PQMLDVPAAGVGAAVGSWIQAHLVNAATGAAVWNDGSEADRLSPGYSFQACLVWLDTG
                     QQTYCSSTSSWAAGSGLHQVTPDGNGVRGGWVDWVETGGASAPSWQRFIDSSAYYILA
                     TTGGYRFAP"
     gene            1814..>2109
                     /locus_tag="PLX85_07920"
     CDS             1814..>2109
                     /locus_tag="PLX85_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRC63131.1"
                     /translation="MDRVEERLGALRDLEVLEARSEPELDGLASLAALACGTSMAAIS
                     FLDGEREWFKATVGFEAASLPMSQSLTRDLVAGSGDALIVSGEAIADRWPVHPLL"
BASE COUNT          356 a          687 c          725 g          341 t
ORIGIN      
        1 ccatagagga cgctcatgct cgcgggcggc atcgcgtagg caagggcggc gttttgccag
       61 cgagaaccga cccagccgga cgcgtaggag acgcccaccg cgtcacctgc tgaactcaac
      121 ccgtacacgt cagctgcgta attgagccca tgcagggctg tcacgtcata catcgcacat
      181 acctccatcc gacggcggta gggtaataac agcgccgaga ggcgctacga acgtgatgtg
      241 tgacgtatgc cgtcggatac agcactgtgc ggacgtgcgc gcgcaccggt tctgcgattc
      301 tggcgtcatg gggacagacc ggcggggcgc agctccgtct cgtgaggcgg cacagcaaga
      361 gcggcgcggg cccaaatccc gggtgctcgt gcgtcttcgc ggcctgcacc tggtgctggc
      421 gacgatgatc ggcatgctcg cgctcgtggt gacgccgcgt ctgcagcact cgggggccgc
      481 gacgccggct ctcgcggcgt gcgcgaacgc cgctgacgtt cagctcgagg ctgactacat
      541 cgtcgcgtcg cagtacgtga agaagaacga ccgtgcgtcc ggtgccatca acaacgtctt
      601 cggcgcaccg acctgggtcg tgccgcgcga gaatgcgatg gcgatccttg ggctcgtgcg
      661 ggcgagcgac tgccttggag cgtcgagcta ccgccagcgc gcacagcagg ccgcggacta
      721 tctcgtgcgg atgcaggcga gcgacggcag ctggtacgac cagtactcgt acgctgcccc
      781 cgtagtcctg agcaagtcgc cgacgcagac cgccgaggtg atgctcgcgc tcgaccggct
      841 cggattcagc cggagctggt acgccgcgat gatggggggc gccgacttcc tcgtgcgcct
      901 gcaggacccc acgaacaagg gcggggccga cgacggactg ctcggcggcg gtctggactc
      961 aaacggcagc tatctccgct ggcgttggac atctgacaac tcatatgggt atcaggcgct
     1021 gaaggccgcg gagaagtggg cgcgcacaca gggcgacgcc gtgcgcgcga atcgctatgc
     1081 ggcggccagc ggtcgcatcc tgaacggcat caatacgcgg ctgaaagacc ccggctcgcc
     1141 agtctggtac ggggcgatcg acgagaacgg cgtgtcaacg ctgatcgagc acgaatggat
     1201 caactacgca ccgcagatgc tcgacgtccc cgccgcgggc gttggcgccg cggtcggcag
     1261 ctggatccag gctcacctcg tgaacgccgc caccggcgct gccgtctgga acgacggctc
     1321 cgaggcggac cgcctttcgc cgggatactc cttccaggca tgtctcgtct ggctggacac
     1381 cgggcagcag acgtactgca gctccaccag ttcgtgggcg gcagggagtg gattgcacca
     1441 ggtgacgcct gacggtaacg gcgtccgtgg aggctgggtg gactgggtgg agaccggtgg
     1501 ggcgagcgcg ccttcctggc agcggttcat cgattccagc gcctactaca tcctggcgac
     1561 gaccggtggc tatcgcttcg cgccgtagtt gcgcgcttcg gacgttcgcg gcatgcttcg
     1621 gcagcatcga gactcgcatg gaactcgcca cgcgtccgcg atgttaagga ctgacagcac
     1681 agcattcgag gactgactac ctcgatgaga ccggcgcctc aatttggtgt tgccgaccgc
     1741 gcccgcttcg cgttccggcc gagcgcgggt cgcgctgtgg cacccgccac gcccgacgat
     1801 gactgagcag atcgtggacc gagtagaaga gcggctcggc gcgctgcgtg acctcgaggt
     1861 gctcgaggcg cgatccgaac ccgagcttga cggcctcgcc tcgctggccg ccctcgcctg
     1921 cggaacgtcg atggcggcga tctcgttcct cgacggagag cgggagtggt tcaaggccac
     1981 ggtcggcttc gaagccgcct ccctgccaat gagccagtcg ctcactcgag acctggtcgc
     2041 gggcagcggc gatgcactca tcgtcagcgg tgaggcgatc gccgatcggt ggcccgtcca
     2101 cccactgct
//
LOCUS       DAORPG010000364         2104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6497_length_2104_cov_3.490971, whole genome shotgun sequence.
ACCESSION   DAORPG010000364 DAORPG010000000
VERSION     DAORPG010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6497_length_2104_cov_3.490971"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1161)
                     /locus_tag="PLX85_07925"
     CDS             complement(133..1161)
                     /locus_tag="PLX85_07925"
                     /inference="COORDINATES: protein motif:HMM:NF041501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGLLP motif-containing membrane protein"
                     /protein_id="HRC63132.1"
                     /translation="MNEATSDFVEDFPAFSQLSGDPITIATNLAMSLGLLFLILLSAT
                     IFNQTLEENHGALARMFSRFAGPFSVLWGRSSVASAGGRILSLVRVGIILLIVAGIYS
                     GLDPDFGFNTSTAALIISMTVGVGLLTILYEGVQVLVAPRAVGTVGHLEPLPVGILIA
                     GLSVVITRITDLHPGIVLGVVAGAIVPTADPRDQGRLVFVATIGVLLLSLVALALVDP
                     LRDYANDHSTTWWAAIPETVAVTLFIGGLEGLFLNMLPLEFMEGHALWKWSKLAWAVS
                     TGVIAFLFLHVVINRTDSYDQVAEETGIRALFLVCAVSLLMAGAFWLVCRIWLHEPEE
                     HAHPTPAA"
     gene            1211..1522
                     /locus_tag="PLX85_07930"
     CDS             1211..1522
                     /locus_tag="PLX85_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63133.1"
                     /translation="MTARVGVGLGPGVADAPGVGEGVAVADGLGVGVEVGRLAATVTA
                     GAATVGDTGVLGDGVGVRVAVGVGDGEDVGVGVGLGVGVGVGVGVGVAVGVARVPVIC
                     T"
     gene            complement(1847..>2104)
                     /locus_tag="PLX85_07935"
     CDS             complement(1847..>2104)
                     /locus_tag="PLX85_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63134.1"
                     /translation="KCDNNGFVIDGFTLNGSGQITQIALSDIDATCVGGQLTVDLTRA
                     DEVSIGNGGPVTVTGSPMSVAITGLPDATLVKHEVVIIVGP"
BASE COUNT          346 a          640 c          788 g          330 t
ORIGIN      
        1 cgaggcttgt tcttgtgcgc cctcggacat catcgccggt gcccatgatg cacgcggcgg
       61 gtttcggccc acctcggggc gcggaagggc caacgtcaaa cggctgccga gaacctgaac
      121 gaaccgcggc gtctaggccg ctggcgtggg gtgggcgtgc tcctcgggct catgcagcca
      181 gatgcggcag acaagccaga aggcgccggc catcagcagc gagactgcgc agacgaggaa
      241 cagcgcgcgg atgcccgtct cctccgcgac ctgatcgtac gaatcggtcc ggttgatcac
      301 gacgtggagg aacaggaagg cgatcacacc cgtgctgaca gcccacgcga gcttgctcca
      361 cttccagagc gcgtggccct ccatgaactc gaggggcagc atgttgagga acagtccctc
      421 gagtccaccg atgaacagcg tcacggctac cgtctccggg atagccgccc accaggtcgt
      481 cgagtgatcg ttggcgtaat cgcgcagggg atcgaccagc gcgagcgcga cgaggctgag
      541 cagcagcact ccgatggtcg cgacgaagac cagtcgaccc tggtcccggg ggtcggctgt
      601 cgggacgatc gcgccggcca ccacgccgag gacgatgccc ggatgcaggt ccgtgatgcg
      661 ggtgatgacc actgaaaggc ccgcgatcag gatgccgacg ggcagcggct cgaggtgccc
      721 taccgtgccg acggcgcgag gggcgaccag gacctgcacg ccctcgtaca ggatggtgag
      781 caggcccacg ccaaccgtca tgctgatgat gagcgcggcc gtcgaggtgt tgaagccaaa
      841 gtccgggtcg aggccggagt agatgcccgc cacgatcaac aggatgattc cgactcggac
      901 aagggacagg atgcggcctc ctgcgctcgc gacgctgctc cgaccccaga ggacgctgaa
      961 cggaccggcg aaccggctga acatccgggc gagggccccg tgattctcct cgagggtctg
     1021 gttgaagatg gtggccgaca gcaggatgag gaacagcagc ccgagtgaca tcgccaggtt
     1081 ggtggcaatc gtgatcgggt cgcccgagag ctgactgaac gccgggaagt cctcgacgaa
     1141 gtcggacgtc gcctcgttca cctctggctt tggcggctcc tccctggtgc tcccggccgg
     1201 cggcgggttc gtgactgccc gggttggggt cgggctcggt cccggtgtcg ccgacgcgcc
     1261 cggcgtgggc gaaggcgttg cggtcgctga cggcctcggg gtgggggttg aggtcggcag
     1321 gctcgcagcc accgtcaccg cgggcgcggc gaccgtcggc gacaccggcg tgctcgggga
     1381 cggggtgggc gtgcgggtcg ccgtcggcgt gggtgatggc gaagacgtcg gcgtgggagt
     1441 cggactcggc gtcggggtgg gcgtcggtgt cggcgtgggg gtcgcggtgg gcgttgcgag
     1501 ggtgcccgtg atctgcacat gaatgccagt gacgtcgctc acgagtggcg gactgtccat
     1561 cgctaccgtc agtgtcggac cgctgatcgc ggcgcttcca ctggcgatcg tcgaaccgcc
     1621 atttttcagg ctgacgctgg ccgtgccgtt gcacgtggag gcgatgtccg tgaggcggac
     1681 agcgcttact cgataggccg ggggggagcc ggtgaacgac aggtcgtacg aggcgtggat
     1741 gccgtccggg tcgcagccgg cctgcgcttc ggcggcggtg gtggagagca gcaagagggc
     1801 gcagctaatg gccgcgagca gggcgggtaa cgcgagacgg cgcacgctag gggccgacaa
     1861 tgatcacgac ctcgtgtttc acgagcgtgg cgtcggggag gccggtaatg gcgacgctca
     1921 tcggcgagcc ggtcacggtg acgggaccgc cgttgccaat gctgacctcg tccgcccgcg
     1981 ttagatcgac ggtgagctgc ccgccgacgc aggtggcgtc aatgtcgctc aacgcgatct
     2041 gcgtgatctg accgctgccg ttgagggtga aaccgtcgat cacgaagccg ttgttgtcgc
     2101 actt
//
LOCUS       DAORPG010000365         2100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6518_length_2100_cov_3.847433, whole genome shotgun sequence.
ACCESSION   DAORPG010000365 DAORPG010000000
VERSION     DAORPG010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6518_length_2100_cov_3.847433"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..747)
                     /locus_tag="PLX85_07940"
     CDS             complement(97..747)
                     /locus_tag="PLX85_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63135.1"
                     /translation="MGRSRRPLLIEVLLASFGMLLFACSSSAGRVLTPSPTRTPTPIE
                     VTPLPEGLDESQAVAALRARPLRLPLPGEVACPASAARPLPAPVLGEAYGDGPLYLLA
                     GGTGPAVLTFDEQHAVKAIWQLTAASEGPVLIRGSALSGQAALAFDDGAAELVFPKGG
                     APRGPGAAAGWHELASVIRIAAPGCYGLQMDGPDFSSVIVFEARSDSSPRYDSSPR"
     gene            885..1289
                     /locus_tag="PLX85_07945"
     CDS             885..1289
                     /locus_tag="PLX85_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008125337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRC63136.1"
                     /translation="MPARSAARPADPRDALFRALADPTRREVVERLGRGAASVSQLAG
                     GFDMALPSFVEHLRVLESSGLVRSQKHGRVRTYELAPEGLRPLEDWLAAQRRRWERRL
                     DQLDDYLLQLKAAEERRAQPSAAPRTQKGTPS"
     gene            1286..1870
                     /locus_tag="PLX85_07950"
     CDS             1286..1870
                     /locus_tag="PLX85_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRC63137.1"
                     /translation="MRKLIVSEFTSLDGVMQAPGGPEEDTDGGFAHGGWTLPYWHDDI
                     GARFFEAMAECDAFLLGRRTWESHSIFETFEEGDPFGDLMKGMRKYVVSTTLESADAW
                     RNSTIIRDNVVEEVRRLKQQEGKSIYIDGSSVLIHELARHGLIDEYSLLVYPLVLGGG
                     KRVFEDGPRVDMALVETRTFPTGVVLMRYAVNHD"
     gene            1914..>2100
                     /locus_tag="PLX85_07955"
     CDS             1914..>2100
                     /locus_tag="PLX85_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002107662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRC63138.1"
                     /translation="MTSIGGFEIDPRLDLVLERTVDVRPALVWKAWTEPEHMKPWFCP
                     RPWSLAECEVDLRPGGKF"
BASE COUNT          331 a          794 c          688 g          287 t
ORIGIN      
        1 gctcccggcc gtggaggcgt cagggtcggc gttcccgcca tgttcgccga cccggccgag
       61 gagcaacagg ggccagccct accgcggcga ggagtcctac cgcggcgagg agtcgtaccg
      121 cggcgaggag tcggaccgcg cctcgaacac gatcaccgag ctgaagtcgg ggccgtccat
      181 ctggagtcca tagcaacccg gagccgcgat gcgaatgacc gaggccagct cgtgccaccc
      241 ggcggcagcg cccggccccc gaggcgcgcc gcccttcggg aagacgagtt cggccgcgcc
      301 atcgtcgaac gccagcgcgg cctgtccgct cagggcgctc ccccggatca gcaccggccc
      361 ctccgaggcg gccgtgagct gccagatcgc cttgaccgcg tgctgctcgt cgaaggtgag
      421 gacggctggc cccgtgcctc ctgcgagcag atacagcggg ccgtcgccat acgcctcccc
      481 gaggaccgga gcgggcaacg gtcgcgccgc cgaggcggga caggcgacct cgcccggcag
      541 cgggagccgc aacggccgtg ctcgcagcgc cgccaccgcc tgcgactcgt cgagtccctc
      601 cggaaggggc gtgacctcaa tcggcgtcgg cgtgcgcgtc ggcgatggcg tcagaacccg
      661 acccgccgac gaggagcacg cgaacagcag catcccgaac gaggcgagca ggacctcaat
      721 cagcagcgga cgtcgtgagc ggcccatcga ctcgctcccg tctcccccgg ccgggcgccc
      781 ccggcgctcg tcgagtgtac gcaccggcac ggcgcccgga tgcgaataca cttaggtgtt
      841 gacctaactg tttttgatgc gctacggtca ctcgaggagg agcgatgccg gcccgatccg
      901 cagcccgacc cgccgacccg cgcgacgccc tgtttcgcgc cctcgcggac cccacccgtc
      961 gcgaggtcgt cgaacgtctc ggccgcggcg ccgcctcggt cagccagctc gccggcggct
     1021 tcgatatggc gctgccctcg ttcgtcgagc acctgcgcgt cctcgagtcc tcgggcctcg
     1081 tccgctccca gaagcacgga cgcgtccgca cctacgagct cgcccccgag ggcctgcgtc
     1141 cgctcgagga ctggctcgcg gcgcagcggc gccgttggga acgccgcctc gaccagctcg
     1201 acgattacct gctccagctc aaggcggccg aggaacgccg cgcgcagccc tcggcggccc
     1261 cgcgcaccca gaaaggaaca ccgtcatgag gaaactgatc gtctccgagt tcacgtcgct
     1321 cgacggcgtc atgcaggcgc ccggaggtcc cgaggaggac accgacggcg gcttcgccca
     1381 cggtggttgg acgctcccgt actggcacga cgacatcggt gcgcgcttct tcgaggccat
     1441 ggccgagtgc gacgcgttcc tcctcggccg ccgcacctgg gaaagccaca gcatcttcga
     1501 gacgttcgag gaaggcgacc ccttcggcga cctcatgaag ggcatgcgga agtacgtcgt
     1561 gtccacgacg ctggagtccg cggacgcctg gcgcaattcg acgatcatcc gtgacaacgt
     1621 ggtcgaggaa gtgcgccggc tcaagcagca ggaaggcaag tcgatctaca tcgacggcag
     1681 cagtgtcctc atccacgagc tcgcccgcca cggcctcatc gacgagtaca gcctgctcgt
     1741 ctatccgctc gtcctcggcg gcggcaagcg cgtgttcgag gacggtcccc gcgtcgacat
     1801 ggcactcgtc gagacgcgga cgttcccgac cggcgtcgtc ctcatgcgct acgccgtcaa
     1861 ccacgactga cccgcatcgc ccgtccccgg gccccggcag caaggagcga cccatgacct
     1921 cgatcggcgg cttcgagatc gacccccgac tcgacctcgt cctcgagcgc accgtcgacg
     1981 tgcgccccgc cctcgtctgg aaggcctgga ccgagcccga gcatatgaag ccctggttct
     2041 gccctcgccc ctggtcgctg gccgaatgcg aggtcgacct ccgccccggc ggcaagttcc
//
LOCUS       DAORPG010000366         2098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6527_length_2098_cov_4.114537, whole genome shotgun sequence.
ACCESSION   DAORPG010000366 DAORPG010000000
VERSION     DAORPG010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6527_length_2098_cov_4.114537"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..382)
                     /locus_tag="PLX85_07960"
     CDS             complement(137..382)
                     /locus_tag="PLX85_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRC63139.1"
                     /translation="MPIYEYKCRKCGHEFELMRRLSQMDAPAPCPACRSKATGRKISA
                     FVLAGRAKVDLTYDDVDPDEYDLEHTDHGDEPDWADF"
     gene            complement(410..1138)
                     /gene="rpoD"
                     /locus_tag="PLX85_07965"
                     /pseudo
     CDS             complement(410..1138)
                     /gene="rpoD"
                     /locus_tag="PLX85_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913240.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
     gene            complement(1595..1900)
                     /locus_tag="PLX85_07970"
                     /pseudo
     CDS             complement(1595..1900)
                     /locus_tag="PLX85_07970"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
BASE COUNT          301 a          693 c          712 g          392 t
ORIGIN      
        1 tcctcgttgg gttcgtgacg ctgatcgaag gcctggcact cgcgggcgcc gcgtatgcga
       61 cgctgggctg gctcagccag ccggagccag tgaggccgcc cgcaagacgg cccgccgagg
      121 ggaccggggc cgcttcctag aagtcggccc agtccggctc atcgccgtga tcggtgtgct
      181 ccaggtcgta ctcgtccgga tcgacatcgt cgtacgtgag gtcaaccttc gcccgcccgg
      241 cgaggacgaa ggccgagatt ttgcgccccg tcgcctttga acggcaggcg gggcatggcg
      301 ccggcgcgtc catctgcgac agccggcgca tcagctcgaa ctcatgcccg cacttgcggc
      361 acttgtactc gtagatcggc acttcgcccc tccaccgaac caggcgcgac tagtcgaggt
      421 agtccttcag cttcttggag cgcgtcgggt gacgcagctt gcgcagcgcc ttcgcctcga
      481 tctggcggat gcgctcgcgg gtgacgctaa actcgcgccc gacctcctcg agcgtccgtg
      541 aacgcccgtc ttccaggccg aagcggagtt cgagcacctt gcgctccctt ggagtgaggg
      601 aggagaggac gtccatcacc tgctccttca ggagctggtg cgaggccgcg tccgccggcg
      661 ccagcgcgga ctcatccggg aggaagtcac ccaggtggga gtcttcctcc tcgccgatcg
      721 gggtttcgag ggagaccggc tcctgcgaga ccttcatgat ctcgcggacg cggtcgggcg
      781 ggagctcgag ctcgcgccca atttcctcgg aggtgggctc acggccgtat tcctgtacca
      841 ggcggcggga gatgcggacg agcttgttga tcgtctccac catgtgcacg gggatgcgga
      901 tggtgcgcgc ctggtcggcg atcgcgcggg tgatggcctg acggatccac caggtcgcat
      961 aggtcgagaa cttgaagccc ttgcggtagt cgaacttctc gacggcccgg atcagaccga
     1021 tgttgccctc ctggatgagg tcgaggagag acatgccgcg gccgatgtac ttcttggcga
     1081 ccgagacgac gaggcgaagg ttggcctcgg tcagctggcg ctccgagcgc tcgccgtcgt
     1141 aggcgatctt gtcgaagtag tacttgatgg cggtgccgtg atccgcctcg agcttctgca
     1201 cgaggccctt cggaggcggg aagaccttcg actcaccgcc gacctgctcg cccgcccagc
     1261 gcacgtgctc gggccgcatg atgtgggtga tgatggagag gcggatcagc gcggcgtacg
     1321 cgcgctcctc gtcccacttc agctccttca tcagcgcgtc tcgggccggc tcgtcgagct
     1381 cgcggtcgat cgtggagcgg aacttctggt cgacgatccg ctcggagacg gactgccgag
     1441 gcagctcgag gtggcggctc accgcctggt agaccgcgcg ctcctggtgg aactgcttga
     1501 acagctcgat caggatttcg acgtgggagg ggtctcgccc ctccgccttg cggaagtcgt
     1561 ccttgatcgc ctcgacgtgg agccactcct ccatggcgcg ggcgaggcgc ttctcgtctt
     1621 cggccgtgag cagcggcacc ttgccgatct cgcgcaggta catacgcacc gggtcgtcga
     1681 tgccgatggc ctcgtcgacg atgcgtcggg cggcctcgac gactgcgccc gcgccgcggt
     1741 ccatgtcgtt gtcgatgcgg accgtgatcc cgttctcgga caactgcgac acgatcgagg
     1801 agagccgctc gcggctaccc tcggcctcgg ggatggccgc gaggacctca gcggcggtga
     1861 cgtggccgga gcggcgtccc ttgttgagga gctcctccat cccggcctct tcgaaggtct
     1921 gcgtcatatc gatcctctcc atggtcgccg cccgctcatc gggtcgacgc atttactcgc
     1981 ttcgttgatg ttcagacggc gggcgcgcct tcgctctcga ccgccgcttc tccacgtgcc
     2041 cgtcgcgaga gggcgcgctg gcgttgatgc agctcatcga actccgcgac gaggccgt
//
LOCUS       DAORPG010000367         2098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6529_length_2098_cov_3.166422, whole genome shotgun sequence.
ACCESSION   DAORPG010000367 DAORPG010000000
VERSION     DAORPG010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6529_length_2098_cov_3.166422"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1905)
                     /locus_tag="PLX85_07975"
     CDS             complement(<1..1905)
                     /locus_tag="PLX85_07975"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HRC63140.1"
                     /translation="MTCQRCGSPLADDAIFCGSCGTRADGPAPLPGFEAVAVRAAPRS
                     PQRVAPPRPADPAAFPDDLEVYRARLQSVFGYPDFRRGQAEVLTHLAARDVLGVMPTG
                     SGKSLCYVLPALEVGRTLVVSPLIALMQDQVEALQSAGVAATFINSSLDRAEQNRRYR
                     DFIEGRTALLFVAPERFANQRFVEGLQAAGVNLFVVDEAHCISEWGHDFRPDYLALGP
                     VRERLGAPRTLALTATADPLVRRDILARLGVAGRADEVLTTFDRPNLHLGVVPVTSDG
                     ERLDWLVRYARSHRGQAGIIYARTRRNVEDTAAALSAAGVPALAYHAGMGSADRARIQ
                     RRFITGEVPVLVATNAFGMGIDKPDVRYVVHTHLPGRIEAYYQEAGRAGRDGDPAECT
                     LLYARRDAALQRRFIDQAHPDEHELRRIWWRLVELQEYAGDRAIQYGDAVDASASEGL
                     PVALTAFRASGLIEPGALRLTSTDRDAPLDLTPIEERRRYAESRLAQMIEYAETSGCR
                     RALILRYFGEESADRCTNCDNCRGNGIASEPEYPLDLFNDLLDARADLARSSGRPPYL
                     VFEERTAREIATYRPATQDALLEIWGMGETRSRWFGDRLLRLVAAWEAAHADAPRAPA
                     SRPCTAANERP"
BASE COUNT          329 a          650 c          823 g          296 t
ORIGIN      
        1 cgggcgttcg tttgcagcgg tacatggtcg cgacgcgggc gccctcgggg cgtcggcgtg
       61 agcggcctcc caggcggcca cgaggcgcag caggcggtcg ccaaaccagc gagagcgcgt
      121 ttcgcccatc ccccagatct cgaggagtgc gtcctgcgtg gccgggcgat aggtcgcgat
      181 ctcgcgggcc gtccgctcct cgaatacgag gtagggggga cggccggagg aacgcgcgag
      241 gtcggcacga gcatcgagga ggtcgttgaa gagatcgagg gggtactcgg gttccgacgc
      301 gatgccgttg ccacggcagt tgtcgcagtt cgtgcagcgg tcggcggact cctcgccgaa
      361 gtagcgcagg atgagtgcgc ggcgacagcc ggaggtctcc gcatactcga tcatctgggc
      421 gaggcgtgat tccgcgtagc ggcggcgttc ctcgatgggg gtgagatcga ggggggcgtc
      481 ccggtcggtt gaggtgagcc gaagcgctcc gggctcaatc aaaccgctgg cgcgaaaggc
      541 ggtgagcgcc acgggaagcc cctcgctggc gctggcgtcg acggcatcgc cgtactggat
      601 agcgcggtcg ccggcgtact cctgcagctc gacgaggcgc caccagatac gtcgtagctc
      661 gtgctcgtcg gggtgggcct ggtcaatgaa gcggcgctgg agggcggcgt cacgcctcgc
      721 gtagagcagg gtgcactcgg ctgggtcgcc atcgcgtccg gcgcggccgg cttcctggta
      781 gtacgcctcg atacgaccgg ggaggtgcgt atgcacgaca taacggacgt ctggcttgtc
      841 gatgcccatc ccgaaggcgt tggtggcgac gaggacgggg acttcgccgg tgatgaagcg
      901 gcgctggatg cgggcccggt cagccgaccc catacccgcg tggtatgcga gcgccggcac
      961 gccggcggcg gacagggcgg cggcggtgtc ctccacgttg cggcgggtcc gcgcgtagat
     1021 gatcccggcc tgaccccggt ggctgcgcgc gtagcgcacg agccagtcca ggcgctcgcc
     1081 atcgctcgtc accggcacca cgcccaggtg gaggttgggg cggtcgaacg tcgtcagcac
     1141 ctcatcggcg cgcccggcga cgcccaggcg cgcgaggatg tcacggcgta ccagcgggtc
     1201 tgcggtggcc gtgagcgcga gcgtcctcgg cgcgcccagg cgctcacgga cgggccccag
     1261 agccaggtag tcgggacgga agtcgtggcc ccactcggag atacagtgcg cctcgtcgac
     1321 gacaaacagg ttgacgcccg cagcctgcag gccttcgacg aaacgctggt tggcgaagcg
     1381 ttcgggcgcg acgaagagca gcgccgtgcg cccctcgatg aagtcgcggt agcgacggtt
     1441 ctgttcggcc cgatcgagcg atgagttgat gaacgtcgcg gcaacgccgg cggactgaag
     1501 cgcctcgacc tgatcctgca taagggcgat cagtggggag acgaccaggg tgcgaccgac
     1561 ctcgagtgcg ggcaggacat aacacaagct cttcccggag ccagtgggca ttacgccgag
     1621 gacatcgcgg gccgcgaggt gggtgagcac ctcagcctga ccgcggcgga aatcggggta
     1681 gccgaagacc gactgcaggc gggcccggta gacctcgagg tcgtcaggga aggctgctgg
     1741 atcggccggg cggggcgggg cgacgcgctg gggcgaacgg ggagcggcgc ggacggcaac
     1801 ggcctcgaag ccgggtagcg gggcgggacc gtcggcgcgg gtgccgcagg agccgcagaa
     1861 gatggcgtcg tcggcaaggg gtgagccgca gcgctggcag gtcaccggcg catcgtaccg
     1921 cacatgtgtg ctaggtggac gcggtgacgc ccgcgagctc ctcggcgcgg gcgacgaggc
     1981 cggggacatc gacgatttcg ccctcgaccg tgaggtgttg gccctcgcgg gggaggaact
     2041 tccagcggac gttgcccagg gtctcgatac gctcgatgcg ctcgacgtcg gcccaggc
//
LOCUS       DAORPG010000368         2091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6564_length_2091_cov_3.336444, whole genome shotgun sequence.
ACCESSION   DAORPG010000368 DAORPG010000000
VERSION     DAORPG010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6564_length_2091_cov_3.336444"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..515
                     /locus_tag="PLX85_07980"
     CDS             183..515
                     /locus_tag="PLX85_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63141.1"
                     /translation="MGLRSLLLTILSLIWAAILVLVGGRFLALLVDANRDSEIVDRLI
                     RHSDLWVRPFTGLFGLTNEAVNSTGGVFEAASAIAFGFYLVVGLLVFALVNSVSGFGP
                     RYRGPVEG"
     gene            complement(778..1593)
                     /locus_tag="PLX85_07985"
     CDS             complement(778..1593)
                     /locus_tag="PLX85_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC63142.1"
                     /translation="MGTEQMVLAIDDEPGILRVIKLELSAQGFRVLTAGSGPEGLRMA
                     EEHRPDLVLLDIVMPEMNGLEVMRELRERSNVPIIMLTAKRSETDKVMGLEMGADDYL
                     PKPFSLDELSARVRAVLRRSSATPNVESVVRVEDVEIDLGRRLVKRDGELVNLTRTEW
                     MLLQCLAANAGKVMLNAELLSKVWGPEYVGDLQYLRVWVSRLRGKLEREPSEPKIIRT
                     FQGVGYMLSSDESEKSGNEAGSSDSEAAAGGPSTAAASAPVGAGEAAPLDLSA"
BASE COUNT          301 a          745 c          668 g          377 t
ORIGIN      
        1 cgggaggggg cgcacggcaa ccgttgcccg gcctgtggcg ccgactgcac gacggaggcc
       61 ctcctggcga atcaccgcga gagctaccac ggtcgccgtg acgacgaggc tctcgcacgt
      121 cagcagggag aactcaagcc gtaagccgtg gcgttctcgc ctccgcatgg aaaggaactg
      181 taatgggact gcgctcgctg ctgctcacga tcctgagtct gatctgggcg gcgatcctcg
      241 tcctcgtcgg tggccgcttc ctggccctgc tcgtcgacgc caaccgcgac agcgaaatcg
      301 tcgaccgcct gattcgccac agcgatctct gggtgcggcc gttcaccggg ctcttcggcc
      361 tgacgaacga ggccgtgaac agcacgggtg gtgttttcga ggccgcctcg gccatcgcgt
      421 tcggcttcta cctcgtcgtg ggactgctgg tgttcgcgct cgtgaactcc gtcagcggct
      481 tcggcccccg ctatcgcggt ccggtcgagg gctagcgagg cgtcagcatg cggcgcgcct
      541 ccgcattcct gctgctgact gccgcggcgc ttgcgttcgt gccctcgcgc gcgcggccgc
      601 gaacagttgc cggctcgcgg cggctgacgc gggaccttcg ccggcgtcga gaccagcggc
      661 gcatcgagcg cgatctgagg cgactcgagc cggccgagta ggcccgtcac gagcaacgac
      721 gaaagcggca gggttccacc ccgccgcttt cgtcatgtgt ggcacgccag cgccgcctca
      781 ggcgctgaga tcgagcggag cggcctctcc ggcgcccacg ggcgcactgg cagccgcggt
      841 cgaaggcccc ccggctgcgg cctcggagtc ggacgagccg gcctcgttcc cactcttctc
      901 gctctcgtcg ctgctgagca tgtagccgac gccctggaag gtccggatga tcttcggctc
      961 agacggttcg cgctcgagct tgcctcgcag gcgggacacc cagacgcgca ggtactgcag
     1021 gtcgccgacg tactcggggc cccagacctt gctcaacagc tctgcgttga gcatcacctt
     1081 cccggcgttc gcggccaggc actgcaggag catccactcg gtgcgcgtga ggttcaccag
     1141 ttcgccgtcg cgcttcacaa gccgtcgccc gaggtcgatc tccacgtcct cgacgcggac
     1201 gacgctttcg acgttcggcg tggcgctgct ccgccgcagc acggcgcgca cgcgcgcgct
     1261 cagctcatcg aggctgaacg gcttcggcag gtagtcgtcg gcccccatct ccaggcccat
     1321 gaccttgtcg gtctcgctcc gcttcgccgt cagcatgatg atcggcacgt tggagcgctc
     1381 tcgcagctcc cgcatgacct cgaggccgtt catctcgggc atcacgatgt cgagcagcac
     1441 gaggtcgggg cggtgctcct cggccatgcg caggccctcc gggccgctgc ccgcggtcaa
     1501 tacccgaaac ccctgcgcgg agagttcgag tttgatcact cgcaagatgc ccggttcgtc
     1561 gtcgatcgcg aggaccatct gttctgtacc cacgggcttc ctcctcgctc tgctgcgttc
     1621 gccacgcagt cagtggacac gcctggctcg gtacgaccgc ccgtgcgcat ttgcctgggg
     1681 caggtcccgc tgatcgaggc ggtccatgca cgggggcgac ggtcgactcg ctgccgatgc
     1741 tagaggttgc ttccgctgag aattgtaaca agcgtgaagg gcggcatttc agccacatcg
     1801 cgatgccgct tcttcgaatc gcaacggttt tgtcaggtcg cccccgcccc gatcacgctg
     1861 ctgccatctc ggccgggcta ctctcgtgca gtaccgagcg tacaactgac gccacgggct
     1921 ccactcgccc cactcctggg acctgaggcg tcggattgta gagaagcccc gtcgaggaaa
     1981 ctcgacgggg cttctacatg cgagccctgc aggccatctg acccacccac gatgccagaa
     2041 gctggtggct agttgccccc gccgccgttg ccattcccgt ttccgccgcc c
//
LOCUS       DAORPG010000369         2087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6587_length_2087_cov_2.906004, whole genome shotgun sequence.
ACCESSION   DAORPG010000369 DAORPG010000000
VERSION     DAORPG010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6587_length_2087_cov_2.906004"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PLX85_07990"
     CDS             <1..826
                     /locus_tag="PLX85_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRC63143.1"
                     /translation="IVQTYGGNPTELAFDFGTAADPARIDAALAADPELKAVLVTHNE
                     TSTGVTNPLKEIAEVVRKHDRLLIVDAISSLSSIPVETDAWGLDVVVSGSQKGWMVAP
                     GLAFISVSPRAWEAQAKCTTPRFYFDLQRARSSAEKGQTPWTPAVSLYFQLDRALQIL
                     FAEGLENVYARHHRLAEKTRRGLLDLGLELFAAEGHRSDTVTAVKVPEGVDGSLITKT
                     ARTEFGTVLAGGQGSLSGKIFRFGHLGLVSDEDVDNGLRAVAQTMERLGYKTPTPA"
     gene            840..>2087
                     /gene="serA"
                     /locus_tag="PLX85_07995"
     CDS             840..>2087
                     /gene="serA"
                     /locus_tag="PLX85_07995"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRC63144.1"
                     /translation="MARILIADTLAQQGIDLLSQHHDVEVRVGLSEDELVAVVADIQA
                     LVVRSQTQVTRRVLEAAGQLEVVGRAGVGVDNIDVEAATQRGVTVVNAPLANTMSAAE
                     HAFALMLALARNIPQAHAALQAGRWDRNKYMGIELAGRTLGIIGLGRIGTEVARRARA
                     FQMHVLAFDPYVSNDRASALGVELRDLDALLAESDFVTLHTALHEGTRGLINAERLAQ
                     AKPGIRIINTARGALIDEQALFDAVASGQVAGAAIDVFSKEPAVGNVLTTSDRIVVTP
                     HLAASTIEAQDRAAISVAEELMDVLDGKPAQFAVNAPLVDPETMAVIGPYLDAAELAG
                     RVAAQLSSSQLERVRIEYLGEIGNYEVRPLRASVIIGMLERVSTQKVTVVNADALAEQ
                     RGIRIEQESGPAREPYANLVAVQV"
BASE COUNT          343 a          714 c          701 g          329 t
ORIGIN      
        1 aatcgtccag acctacggcg gcaatccgac cgagctcgcg ttcgacttcg gcaccgccgc
       61 cgacccggcg cgcatcgacg cagccctcgc ggccgaccca gagttgaagg ccgtcctggt
      121 cacgcacaac gagacctcca ccggcgtgac caacccgctg aaggagatcg cggaggtcgt
      181 ccgcaagcac gaccggcttc tgatcgtcga cgcgatctcg tcgctgtcct cgatccctgt
      241 tgagaccgac gcctggggcc tggacgtagt cgtctctggg tcacagaagg ggtggatggt
      301 cgccccgggc ctcgccttca tcagcgtgag cccgcgcgcg tgggaggcgc aggcaaagtg
      361 caccacgcca cgcttctact tcgacctgca gcgtgcccgc agcagtgccg aaaagggcca
      421 gacgccctgg acgccggcgg tttctctgta cttccagctc gaccgcgctc ttcagattct
      481 cttcgccgag ggcctcgaga acgtctacgc gcggcaccac cgcctcgctg agaagacgcg
      541 tcgagggctc ctcgacctcg gcctcgagct gttcgccgcc gagggacacc gctccgacac
      601 ggtgaccgca gtgaaggtcc ccgagggtgt ggacggttcg ctcatcacga agacggcgcg
      661 caccgagttc ggcaccgtcc tcgcgggcgg ccaggggtcg ctgagcggga agattttccg
      721 cttcggtcac ctcggtctcg tgtccgacga ggacgtcgac aacgggctgc gcgcggtcgc
      781 gcagacgatg gaacgcctcg gctacaagac gccgaccccg gcctgaacgg ggtcgaccag
      841 tggcacgcat tctgatcgct gacactctgg ctcagcaggg tatcgacctg ctgagccagc
      901 accacgacgt cgaggtccgt gtcgggctgt ccgaggacga actcgtcgct gtggtggcgg
      961 acatccaggc gctcgtcgtc cgcagccaga cgcaggtgac gcgtcgcgtc ctggaagccg
     1021 ccgggcagct tgaagttgtc gggcgggcgg gcgtcggggt cgacaacatc gacgtcgagg
     1081 cggccacgca gcggggcgtg acggtggtca acgcgccgct tgccaacacc atgtccgcag
     1141 cggagcacgc gttcgcgctc atgctggccc ttgcccgcaa catcccgcag gcacacgcgg
     1201 cactgcaggc cggccgctgg gaccgcaaca agtacatggg catcgagctt gccggccgca
     1261 cgctcgggat catcgggctc gggcgcattg gcactgaggt cgcgcggcgt gcacgcgcct
     1321 tccagatgca cgtgcttgcc ttcgacccgt acgtctcgaa cgatcgtgcg agtgcgctcg
     1381 gcgtcgagct gcgggacctg gatgcgctgc tcgcggagtc ggactttgtc acgctgcaca
     1441 cggccttgca tgagggcacc cgcgggttga tcaacgccga acgtctggcc caggcgaagc
     1501 ccgggatccg catcatcaac accgcccgcg gtgccctgat cgacgagcag gcgctcttcg
     1561 atgcggtcgc atcggggcag gttgccggcg ccgcgatcga tgtcttcagc aaggagcccg
     1621 ccgtaggcaa cgtcctcacg acgtcggacc gcatcgtggt cacgccacac ctcgctgcct
     1681 cgaccatcga ggcgcaggat cgtgccgcga tctccgtcgc cgaggagttg atggacgtcc
     1741 tcgacggtaa gccggcgcag ttcgccgtga atgcgcccct ggtggatcca gaaacgatgg
     1801 cagtgatcgg gccctatctg gacgccgccg agctcgccgg tcgcgtcgca gcccagctgt
     1861 cctcgagcca gctcgagcgc gtgcggatcg agtacctcgg tgagatcggg aactacgagg
     1921 tgcggccgct ccgcgcttcg gtaatcatcg gcatgctgga gcgcgtaagc acccagaagg
     1981 tgaccgtcgt caacgcggac gccctggcgg agcagcgcgg catccgcatt gagcaggaga
     2041 gcgggcctgc tcgcgagccg tacgccaacc tcgtagcggt gcaggta
//
LOCUS       DAORPG010000370         2085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6598_length_2085_cov_3.193596, whole genome shotgun sequence.
ACCESSION   DAORPG010000370 DAORPG010000000
VERSION     DAORPG010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6598_length_2085_cov_3.193596"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PLX85_08000"
     CDS             <1..475
                     /locus_tag="PLX85_08000"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRC63145.1"
                     /translation="GLAVVDAKRTHEPAAEPLLAALRRPIARVDVGLAAVLRGVQCGI
                     DISDGLAQDLGHVAKRSNVGIEIDLERLPLHIHAMAMFGIDAARNYAISGGDDYELAL
                     IGRGDIIESLGLPLTVIGRVVADHPGSAVVLRADGRTYEPPPGWDQIRMQPWSSA"
     gene            501..998
                     /gene="tsaE"
                     /locus_tag="PLX85_08005"
     CDS             501..998
                     /gene="tsaE"
                     /locus_tag="PLX85_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRC63146.1"
                     /translation="MNTANRVVITTTTGAKTRALGRRLARLLRAGDVLLLQGPLGAGK
                     TTLAQGIGAGLKVDEPVRSPTFVLMTRHEGGALPLYHADLYRLETVEEVEDLSLDEQA
                     ADGVLLVEWPERGLDALPAHHLLVVLEPVPDEPTWRRITVVAVGDRYRRVLDGLLAAG
                     LADRV"
     gene            complement(1138..1302)
                     /locus_tag="PLX85_08010"
     CDS             complement(1138..1302)
                     /locus_tag="PLX85_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63147.1"
                     /translation="MALNADWHASHPMPPRATFEERAHWHLEHSEACGCRPIPAKLAA
                     QMRERGLLSE"
     gene            1348..1737
                     /locus_tag="PLX85_08015"
     CDS             1348..1737
                     /locus_tag="PLX85_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC63148.1"
                     /translation="MPDTRDMASVRYIIEDVPAALDFYTNALGFEVLTNAAPAFADVA
                     RGHLRLLLSGPQSSGARALPDGRQPVPGGWNRIHLIVDDLEAEVARLRERGVTFRSDI
                     VRGPGGAQCVLDDPSGNPIELFQPASR"
     gene            1752..>2085
                     /gene="tsaB"
                     /locus_tag="PLX85_08020"
     CDS             1752..>2085
                     /gene="tsaB"
                     /locus_tag="PLX85_08020"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRC63149.1"
                     /translation="MAELGIDTASQTIALAVLEGERVLAERRWRLETTASRELLAGID
                     ELLRDAGIERDAIEAIAVNVGPGAYTGLRSGVATAQGLALGLGVPLAGVARLEADAFP
                     HVAPGVPVV"
BASE COUNT          293 a          816 c          664 g          312 t
ORIGIN      
        1 cggcctcgcg gttgtcgacg cgaagcgcac gcatgagccg gccgccgagc cgctgctcgc
       61 cgcgcttcgt cgccccatag cgcgcgtcga tgtcggcctc gccgcggtcc ttcgaggcgt
      121 gcagtgcggc atcgacatct cggacggcct cgcgcaggac ctcggccatg tggcgaagcg
      181 ctccaacgtc ggcattgaga tcgacctcga acggctgccg ttgcacatcc acgccatggc
      241 gatgttcggc atcgacgcgg cgcgcaacta cgccatcagc ggcggcgatg actacgagct
      301 cgcgctgatc ggccgcggcg acatcatcga gtcactcggc ctgccgctca ccgtcatcgg
      361 ccgcgtggtg gcggaccacc ccggcagcgc cgtcgtcctc cgtgccgacg gccgcacgta
      421 cgagccgcct cccgggtggg accagattcg tatgcagccc tggtcctcgg cctgacgcgc
      481 cgaggctcct cgaactcccg atgaataccg ccaaccgcgt ggtgatcacg acgacgaccg
      541 gcgcgaagac gcgcgccctc gggcggcgtc tcgcgcgcct gctccgagca ggcgacgtgc
      601 tgctcctcca gggcccgctc ggcgccggca agaccacgct cgcccagggc atcggcgccg
      661 gcctcaaggt cgacgaaccc gtccggagcc cgaccttcgt cctcatgacc cgtcacgagg
      721 gcggtgcgct gcccctctac cacgccgacc tctaccgcct cgagaccgtc gaggaggtag
      781 aggacctctc cctcgatgag caggcggccg acggcgtcct ccttgtggag tggcccgagc
      841 gtggcctcga cgccctcccc gcgcaccacc tgctggtcgt cctcgaaccc gtccctgacg
      901 aacccacttg gaggcgcatc accgtcgtcg cagtcggcga ccgttaccgc cgcgtcctcg
      961 acgggttgtt ggccgccggg ctcgcggatc gggtgtgacc ggccgattac tgctgtcgtc
     1021 gcgctcgaac tcgagcgcgt cctcggcatt cctgccctgc tctggctgcg tcaacaggct
     1081 cggtatgacc tctacctcgc gcgcgaggtc gaggccgccc gtagtgcctg acatctgcta
     1141 ctccgacagc agcccgcgct cccgcatctg cgccgccagc ttcgccggga tcggccggca
     1201 gccgcacgcc tcgctatgct cgaggtgcca gtgcgcgcgc tcctcgaacg tcgctcgagg
     1261 tggcatcgga tgcgaggcgt gccagtcagc gttcagcgcc acgcgtacag tgtagggcgg
     1321 tgacgaccac agaaaggcgg caacccgatg cccgatacac gtgacatggc cagcgtccgc
     1381 tacatcatcg aggacgtgcc cgccgccctc gacttctaca ccaacgccct cggcttcgag
     1441 gtgctcacga acgccgcgcc cgccttcgcc gacgtggcaa ggggccacct gcgcctgctc
     1501 ctcagcggtc cgcaaagctc gggcgcccgc gcccttcccg acggccgtca gccggtcccc
     1561 ggcggctgga acagaatcca cctgatcgtc gatgatctcg aggccgaggt cgcccgcctc
     1621 cgtgagcgcg gtgtgacctt ccgcagcgac atcgtccgcg gcccgggcgg cgcccagtgc
     1681 gtcctcgacg acccgtccgg caacccgatc gagctgttcc agcccgcaag ccgctagctc
     1741 gaaagcgacc catggccgaa ctcggcatcg acaccgcctc gcagaccatc gccctcgccg
     1801 tactcgaagg cgagcgcgtg ctcgccgagc gccgctggcg cctcgagaca accgcctcgc
     1861 gcgagctgct ggcgggcatc gatgagctgc tgcgggacgc tggcatcgag cgcgacgcca
     1921 tcgaggcgat cgcggtgaac gtcggtcccg gcgcctacac cggcctccgc tccggcgtcg
     1981 ccaccgcgca gggcctcgcg ctcggcctcg gcgtgccact cgccggcgtc gcccgtctcg
     2041 aggccgacgc cttcccccac gtcgcaccgg gcgtacccgt cgttg
//
LOCUS       DAORPG010000371         2083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6611_length_2083_cov_3.703156, whole genome shotgun sequence.
ACCESSION   DAORPG010000371 DAORPG010000000
VERSION     DAORPG010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6611_length_2083_cov_3.703156"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PLX85_08025"
     CDS             complement(<1..570)
                     /locus_tag="PLX85_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HRC63150.1"
                     /translation="MSRILIVSNRLPVTLRVEDDRPILEPSLGGLATGLASLMDSPTT
                     LWIGWPGDLSELTERQRAAVRADLDRQRLVPVELTAEQIQRYYEDYSNGVIWPLFHHL
                     PDRVPYEADGWEVYEEVNALFADAIARVYRPGDQVWIHDYQLMRAPALLRDRVPDAAI
                     GFFLHIPFPSSEVFRTLPQRRPILEGLLGA"
     regulatory      complement(721..857)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(1061..1465)
                     /locus_tag="PLX85_08030"
     CDS             complement(1061..1465)
                     /locus_tag="PLX85_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007318031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC63151.1"
                     /translation="MTVSLSTVHILVDDPDAALGFYRDALGLAIRNEVARDGLRWITL
                     ATESQPEVSIVLSQPHAGRSKEDGDAVAALLAKGEMYPVHFRCDDLDATFAKVAATPG
                     VEVLQEPASQPWGARDAAVRDPAGNFLRIEQA"
     gene            complement(1517..1978)
                     /locus_tag="PLX85_08035"
     CDS             complement(1517..1978)
                     /locus_tag="PLX85_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007318032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC63152.1"
                     /translation="MTVNLDELRLLRRARDQMDRDYAQPLDVPALARTALMSPAHFSR
                     RFREAYSETPYSYLMTRRIERAKALLRRGDLPVTDVCFAVGCTSLGSFSARFTDLVGE
                     TPSAYRARNHDELRGVSPCRLMVLTRPRKDRPTGAAPQMSSFREATPDARS"
BASE COUNT          300 a          557 c          909 g          317 t
ORIGIN      
        1 ggcgccgagg aggccctcga ggatagggcg gcgctggggg agggtgcgga agacttcgga
       61 ggaggggaag gggatgtgaa ggaagaagcc gatggcggcg tcggggacgc ggtcgcggag
      121 gagggcgggg gcgcgcatca gctggtagtc gtggatccag acctggtcgc cgggccggta
      181 gacgcgggcg atggcgtcgg cgaagagggc gttcacctct tcatagacct cccagccatc
      241 ggcctcgtag gggacgcggt cggggaggtg gtggaagagc ggccagatga cgccgttgga
      301 gtagtcctcg tagtaacgct ggatctgctc ggcggtgagt tccacgggca cgaggcgctg
      361 gcggtcgagg tcggcgcgga cggcggcgcg ctgccgctcg gtgagctcgg agaggtcgcc
      421 gggccagcca atccagagcg tggtgggcga gtccatgagg gaggcgaggc cggtggcgag
      481 gccgcccaga ctgggctcga ggatgggccg atcgtcctcg acgcggaggg tgacggggag
      541 gcggttggaa acgatgagga tgcggctcac gagcagccgt ccgttcggac cggcggggga
      601 ggcgcgtcga tcgtcacgat tcgttccaga ccatgcgctc tccaacttcg cccgcgttgc
      661 agcctcgacg ccgaggcggg tggcctcggg gcgtggaggg tgccgctgag gtaggagcgc
      721 agaccctacc tccgagcgcc tacggggtta gctgacgggt tcggcgtcgg aagtcgccta
      781 tgcgccgaac cggcgcaatg caccccagat gtggatcccc cgttcccggc agggccggga
      841 ttcggctggc ggatccacga tagcaggggc ggtcaactcg cgaaggggcg ttggtaacgc
      901 ccgttgaggg gcgtccgtca cccgtggcca cgtgtggcag atcgcgagat cgcagcggtt
      961 ctcctcgttt gctccacgcg cgtgcgaagg aagtcgtgga ctgggcgagg ccggcgacgc
     1021 tcacgtgagg agcgccgcct ggcttcggga gggtgggcgg tcaggcctgc tcgatgcgga
     1081 ggaagttgcc ggcggggtca cggacggcgg cgtcgcgcgc gccccagggc tggctcgcgg
     1141 gctcctggag cacctcgacg ccgggggtgg cggcgacctt cgcgaaggtg gcgtcgaggt
     1201 cgtcgcagcg gaagtggacg gggtacatct cgcccttggc gaggagcgcg gcaacggcgt
     1261 cgccgtcttc tttcgagcga ccggcgtggg gctgcgagag gacgatggaa acctcgggct
     1321 ggctttcggt ggcgagggtg atccagcgga ggccgtcccg tgcgacttcg ttgcggatgg
     1381 cgaggccgag ggcgtcgcgg tagaagccga gggcggcgtc ggggtcgtcg acgaggatgt
     1441 ggacggtgct gagggagacg gtcacgtggc gttcctttcg agggcggtcg gggtgtgcgt
     1501 ggccacgagg ggaacgttac gagcgcgcgt cgggcgttgc ttctcgaaaa ctgctcatct
     1561 gcggggcggc gccggtgggg cggtctttgc gcgggcgggt gagcaccatg aggcggcagg
     1621 gtgagacgcc gcggagttcg tcgtggttgc gggcgcggta ggcgctgggg gtttcgccga
     1681 cgagatcggt gaagcgggcg gagaaggagc cgaggctggt gcagccgacg gcgaagcaga
     1741 cgtcagtgac ggggaggtcg ccacggcgca ggagggcctt ggcgcgctcg atgcggcggg
     1801 tcatgaggta ggagtacggg gtttcggagt aggcctcgcg gaagcggcgg ctgaagtggg
     1861 cgggggacat gagggcagtg cgggcgaggg ctggcacatc gaggggctgc gcgtagtcgc
     1921 ggtccatctg gtcacgggcg cgtcggagga ggcgcagctc gtcgaggttt acggtcatgg
     1981 ggacattatg ccgcgcgagg gagggcctgg agtcctcgat gccaggtgtg gtcgttggcg
     2041 ctgtcggggg cctgggcgcg cgcacgcccg tggttgactg acc
//
LOCUS       DAORPG010000372         2071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6672_length_2071_cov_3.255456, whole genome shotgun sequence.
ACCESSION   DAORPG010000372 DAORPG010000000
VERSION     DAORPG010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6672_length_2071_cov_3.255456"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="PLX85_08040"
     CDS             complement(<1..972)
                     /locus_tag="PLX85_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC63153.1"
                     /translation="MYLRRVEVHGFKSFADRQRFEFGPGVTAVVGPNGSGKSNVAEAV
                     RWALGEQSARSLRARKTEDVIFSGSDKRRQLGMAEVTLVLDNSEQWMPVDFSEVSVTR
                     RAYRSGESEYLINGQRVRLMDVHDLFRRAQVGQNSYAMMSQGLVDEVLALRPVERRDL
                     IEEAADVHRHRIELNRAERRLTETRDNLGHVRMLIREVEPRLKQLERQSARAERFKVL
                     QAQLSDALQVYYEHELRAAQDALTAARARHDQQAQAFQSARIEVDSVLGRADAIDGRV
                     HELRMALERAQSVDRTLTEEGLRLQQAVALAEQRLELLAVRREEVEAE"
     gene            complement(975..1730)
                     /gene="rnc"
                     /locus_tag="PLX85_08045"
     CDS             complement(975..1730)
                     /gene="rnc"
                     /locus_tag="PLX85_08045"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRC63154.1"
                     /translation="MVSASATASGDALPGRPSPEQIAQDRLGIAFRDPGLLRVALTHP
                     SYANEHPEQGHDTNERLEFLGDAVLGLIVAESLYADFPDIEEGRLTEWRAQLVCGPTL
                     ARVATRLGLGDLLLLGRGEEATGGRAREGNLERVFEAVVGAVMQDQGLDAARAFAQAS
                     LREEFAALDADPGVLNPKGALQQLVQGLLGRPQYVTIAEEGPEHARRFTIEARIEGEV
                     VGTGVGSSKHQAEKEAARQALAIVRARLAEQAG"
     gene            1923..>2071
                     /locus_tag="PLX85_08050"
     CDS             1923..>2071
                     /locus_tag="PLX85_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HRC63155.1"
                     /translation="MLRVAKATAEAERVELWAVGGAVRNALLGVPVLEVDMATTAKPD
                     QVITAA"
BASE COUNT          315 a          686 c          758 g          312 t
ORIGIN      
        1 ctccgcctcg acctcctcgc ggcggacggc gagcagctcg aggcgttgct cggcaagggc
       61 gacggcctgc tggagtcgga ggccttcctc ggtgagggtg cggtcgacgg actgcgcgcg
      121 ctcgagggcc atgcgcagct cgtggacgcg gccatcgatg gcgtcggccc ggccgaggac
      181 ggagtccacc tcgatgcggg cggactggaa ggcctgcgcc tgctggtcgt ggcgggcgcg
      241 tgcggccgta agggcgtcct gcgccgcgcg cagctcgtgc tcgtagtaaa cctgcagcgc
      301 atcggacagc tgggcctgaa gcactttgaa gcgctcggcc cgcgcggact ggcgttcgag
      361 ctgcttcagg cgcggctcca cctcgcggat cagcatgcgg acgtggccga ggttgtcgcg
      421 tgtctcggtg aggcggcgct cggcgcggtt cagctcgatg cgatggcgat ggacgtccgc
      481 ggcctcctcg atgaggtcac ggcgctcgac aggccggagc gcgagcacct cgtcgacgag
      541 gccctgcgac atcatcgcgt aggagttctg gccgacctgg gcgcggcgga agaggtcgtg
      601 cacgtccatg agacggacgc gctgcccatt gatcaggtac tcgctctcgc ccgagcggta
      661 cgcccgcctg gtgacgctga cctcggagaa atcgaccggc atccattgtt cggagttgtc
      721 gaggacgagc gtgacctcgg ccatgccgag ctggcggcgc ttgtcggaac cggagaagat
      781 cacgtcttcg gtcttgcggg cgcgcagcga gcgcgcagac tgctcgccaa gggcccagcg
      841 gacggcctcc gcgacgttcg acttgccgga gccgttgggt cccacgacgg cggtcacgcc
      901 agggccgaac tcgaaccgct ggcggtcggc gaacgacttg aagccgtgga cctcgacgcg
      961 gcggagatac atcgctagcc ggcctgctcg gcgaggcgcg cccgcacgat cgcgagcgcc
     1021 tggcgggcgg cttccttctc ggcctgatgc ttgctggagc caacgcccgt gccgaccacc
     1081 tcgccctcga tgcgggcctc gatcgtgaaa cgtcgggcgt gctcgggccc ttcctcggcg
     1141 atcgtgacgt actggggccg cccgaggagg ccctgcacga gctgctggag tgcgcccttc
     1201 gggttcagca cgcccggatc agcatccagc gccgcgaatt cctcgcggag cgaggcctgc
     1261 gcgaaggcgc gggcggcatc gagcccctga tcctgcatga cagcgccgac gaccgcctcg
     1321 aaaacacgct caaggtttcc ctcacgcgcg cggcctcccg tggcctcctc gccacgcccg
     1381 agcaggagca gatcgccgag accgagccga gtcgcaacgc gggcgagcgt agggccgcac
     1441 acgagctggg cacgccattc tgtgaggcgg ccctcctcga tgtcggggaa atcggcatag
     1501 agggactcgg cgacgatgag gccgagaacg gcatcgccga ggaactcgag ccgctcattc
     1561 gtgtcgtgcc cctgttccgg gtgctcgttc gcgtacgaag gatgcgtgag ggcaacgcgc
     1621 aagagacccg gatcgcggaa ggcgatgccg agtctgtcct gtgcgatctg ttccgggctc
     1681 gggcgaccgg ggagggcgtc gcctgaggca gtcgcggatg cggaaaccac tcgtccgcct
     1741 ctcccaccgt acgccatccg ccaccgtacg atgttctggg ccagggtcct cgccgcaggc
     1801 cggagtctcg acgagtgcga ggatcgtacg gagcggggtc ccgagtgtca agaacggatc
     1861 atccgcttaa acaccctacc gtgaacgacc tcgaggcggc gctggtcgag ccgctgcggg
     1921 agctgctgcg cgtggcgaag gcgaccgcag aggcggaacg cgtcgagctg tgggccgtcg
     1981 gcggggcggt gcgcaacgcg ctgctgggtg tgccggtgct cgaagtcgac atggcgacga
     2041 cggccaagcc agaccaggtg atcacggcgg c
//
LOCUS       DAORPG010000373         2071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6674_length_2071_cov_2.888393, whole genome shotgun sequence.
ACCESSION   DAORPG010000373 DAORPG010000000
VERSION     DAORPG010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6674_length_2071_cov_2.888393"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..836)
                     /locus_tag="PLX85_08055"
     CDS             complement(213..836)
                     /locus_tag="PLX85_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63156.1"
                     /translation="MDLPTMRTRVRRDLRDEDAANYRWTDSELDRHIARAVQEVSLAA
                     PLEASATLTTTAGSRDLSITTLVSRVSVEAAEYPLGQYPRSLVPFTTWADTLSLLIDG
                     SPLGGESVLVRYTKLHTLDGSGTTLPDRLHDLVATGAAAYAALEWASYATNRVNVGGT
                     ETWRNYHTWAQERMAAFAKALAKLGRERRLRSHRMYQSAVGAVAARD"
     gene            complement(877..1767)
                     /locus_tag="PLX85_08060"
     CDS             complement(877..1767)
                     /locus_tag="PLX85_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HRC63157.1"
                     /translation="MALTLAEGAKLSNDVLLEGVIETVINDSPVLQRLPFVEIVGNGL
                     TYNRENAAATAAFYGVGDTWAENTPTFTQITTALTILGGDADVDNYLARTRNNVQDLE
                     AAVVTLKAKAVQRKFDDTFINGDTGIDTKSFDGVDKQCTAGQTASMGTNGATLTLAKL
                     DEAIDLIRGGKPDILLMSRRTRRSLTSLARASGSGVVEQDRDEFGRMVQFYDGVPIGV
                     NDYISDAKTVGTSSDCSTIYALQFGEGAVVGLTGPGGLTVDRVGSLETKDATRIRVKW
                     YVALAIFNQLKLSRLVGVRP"
BASE COUNT          287 a          642 c          796 g          346 t
ORIGIN      
        1 cgcgccgagg cttcaggaag cctatgcgcg ccccggcctg ctgcgccgca aggagcgtcg
       61 aggtcagcgc gcgcatcggg cgcgacgcct cggcaggttc gcgggtcgga ggggtgggtg
      121 agtcaccggg actcccgtcc tggccacccc cgatccggat tctggccgct ggcatacggc
      181 ttccatatct atggatgctc ctcgcgcctc gatcagtcgc gggcggcgac cgcgccgacg
      241 gcggactgat acatgcgatg cgagcgcaag cggcgctcgc ggccgagttt ggcgagcgcc
      301 ttcgcgaagg cggccatgcg ctcctgcgcc caggtgtggt agttgcgcca cgtctcggtg
      361 ccgccgacgt tgacgcggtt cgtggcgtag gaggcccact cgagggccgc gtacgcggcg
      421 gcgcccgtcg cgacgaggtc gtgcagccgg tcgggcaacg tcgtgccgct gccatcgagg
      481 gtgtgcagct tcgtgtaccg cacgagcacg gactcgccgc cgagcgggct gccgtcgatg
      541 agtagagaaa gcgtgtccgc ccaggtggtg aagggcacga ggctgcgtgg gtattgcccg
      601 aggggatact cggcggcctc gaccgagacg cgcgagacga gcgtcgtgat gctgaggtcg
      661 cggctgcccg cggtcgtcgt gagcgtggct gacgcctcga gcggggcggc gagcgagact
      721 tcctgcaccg cgcgggcgat gtggcggtcg agctcgctgt ccgtccagcg gtagttggcg
      781 gcgtcctcgt cgcggaggtc gcggcggaca cgggtccgca tcgtcggcag gtccatggca
      841 gcacctccgg ggtgcgcgcg ccctcgaggc gtccgcttac ggacggacgc cgacgaggcg
      901 cgacagcttg agctggttga agatcgcgag cgcgacgtac cacttcacgc ggatgcgcgt
      961 ggcgtccttc gtctcgaggg agccgacgcg gtcgacggtg aggccaccgg ggccggtgag
     1021 cccgacgacc gcgccctcgc cgaactgcag ggcgtagatc gtggagcagt ccgaggaggt
     1081 gcccaccgtc ttcgcgtccg agatgtagtc gttcacgccg atcggcacgc cgtcgtagaa
     1141 ctggaccatg cggccgaact cgtcgcggtc ctgctcgacg acgccggagc cggaggcacg
     1201 agcgagcgag gtcagactgc ggcgcgtgcg gcgcgacatg agcaggatgt cgggcttgcc
     1261 gccgcggatg agatcgatcg cctcgtcgag cttggcaagc gtgagggtgg cgccgttcgt
     1321 gcccatcgag gcggtctggc cggccgtgca ctgcttgtcg acgccatcga atgacttggt
     1381 gtcgatgccc gtgtcaccgt tgatgaaggt gtcgtcgaac ttgcgctgga cggcctttgc
     1441 cttgagggtc acgaccgccg cctcgaggtc ctgcacgttg ttgcgggtgc gcgcgaggta
     1501 gttatcgacg tcagcgtcgc cgccgaggat ggtcagcgcc gtcgtgatct gggtgaacgt
     1561 gggcgtgttc tcggcccagg tgtcgccgac cccgtagaag gccgcggtcg cggctgcgtt
     1621 ctcgcggttg taggtgaggc cgttgccgac gatctcgacg aagggcagcc gctgcaggac
     1681 agggctgtcg ttgatcaccg tctcgatcac gccttcgagg agcacgtcgt tggagagctt
     1741 cgcgccttcc gccagtgtca gggccatgtc catcgcctcc ttcgggtacg acgaggaggc
     1801 taggagaaca tatgtcctat cgagaagcgg gaactggcag cgttgagctg gcaggccgaa
     1861 aacgaatgag gcccccacgc gggagcctcg ggattcggag cggcgtactg gctacgggat
     1921 aacgacggtc gtcgtggcct cgtcgccctc gatggtgacc tcgggcgtgg cggtgctggt
     1981 agacgcggga gtcgtcgcgg tcggcgacgg cgaggccgtg gcgctcgcgg tgggcgtcgc
     2041 cgtcggcgtg cgggtcgcgg tgccggtggg c
//
LOCUS       DAORPG010000374         2071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6676_length_2071_cov_2.572421, whole genome shotgun sequence.
ACCESSION   DAORPG010000374 DAORPG010000000
VERSION     DAORPG010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6676_length_2071_cov_2.572421"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..737
                     /locus_tag="PLX85_08065"
     CDS             93..737
                     /locus_tag="PLX85_08065"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HRC63158.1"
                     /translation="MARKVLLACGPLSSALYLAAIDVVARRQHPEYHRFGSQMVSELF
                     AVGSPTRRTQLWLGAIYNALVFGFAAGIWRSARGRRSIRLAAAALAGYGAASTAGGFW
                     GPMDVRGTPNSRRDQRHILVTLAMSGFILATMLLGAMAGGRRFRMYSLATAMAVTLFG
                     GLAGYLARPMPGPTPGVGLAERVNIYASMAWFAALAAALWRAPLRDGEASRQAR"
     gene            complement(759..1220)
                     /locus_tag="PLX85_08070"
     CDS             complement(759..1220)
                     /locus_tag="PLX85_08070"
                     /inference="COORDINATES: protein motif:HMM:NF021538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HRC63159.1"
                     /translation="MDGYDVLKFLHVYSVVLWVGGGALLNIMGTRLARAGTSQQLAEF
                     GAQVEWIGLHIFTPLSVFALIMGILMALNDRWDFGQTWIQLGLGGWFLSFLLGAGFLG
                     PQSGKLGRLVREHGPDHPEVRPRLTRLLLVARLDVVLMLLIVADMTIKPGL"
     gene            1415..1939
                     /locus_tag="PLX85_08075"
     CDS             1415..1939
                     /locus_tag="PLX85_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010347795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRC63160.1"
                     /translation="MSKPRHFSGRLAALSSAAILAVYAVGYTVTEDAAQSAESGPAPT
                     PTPRASADPTTSPTSTARGTSPAQTPTVTVQRATSTATPGASSGAYRDGTYVGFGTGR
                     HGSIEATVTVEGGKITAVQISQCLTRYPCSRIGALPGQVVSRQSAQVDYVSGATDSSR
                     SFIQAVTNALASAN"
BASE COUNT          334 a          632 c          811 g          294 t
ORIGIN      
        1 cgcgcatcac cgaaggcagc gacggtcagg ggagcggttt gaggcgcgcc ttgactttcc
       61 ggttcaggat gatcgggaat cggaggcggg tgatggctcg gaaagtgttg ctggcgtgcg
      121 gaccgctgtc gtccgcactg tatctggcgg cgatcgacgt ggtggcacga cgccagcatc
      181 ccgagtacca ccgcttcggc tcgcagatgg tgagtgagct gttcgcggtg gggtcgccga
      241 cgcggcggac gcagctgtgg ctcggtgcga tctacaacgc gctcgtgttc ggatttgcgg
      301 cggggatctg gaggtcggcg cgcggccggc gctcgattcg gctcgcggcg gcagcgctgg
      361 cggggtacgg agcagcgagc acggccggag gcttctgggg gccgatggat gtgcgaggga
      421 cgcccaactc gcgacgcgac cagcgacaca tcctggtgac gctcgcgatg tccgggttca
      481 tcctcgcgac gatgttgctg ggcgcaatgg ccggtggcag gcgttttcgg atgtactccc
      541 tggcaacggc gatggcggta acgctgttcg gagggctagc cgggtacctc gcgcggccga
      601 tgccggggcc gacccccggg gtcggcctgg ccgagcgggt aaacatctac gcctcgatgg
      661 cgtggttcgc ggcgctcgcg gccgcgctct ggcgcgcgcc gctgcgagac ggcgaggcgt
      721 ctcggcaagc tcgctgacgc ggcgagggcg ggatgcggct agaggccggg cttgatggtc
      781 atgtcggcga cgatgaggag catgaggacg acgtcgaggc gggcgacgag gaggaggcgg
      841 gtgagcctcg ggcggacctc cgggtgatcg gggccgtgct cgcggacgag acggccgagt
      901 ttgccggact gggggccgag gaagccggcg ccgaggagga aggagaggaa ccagccgccg
      961 agcccgagct ggatccaggt ctggccgaag tcccagcgat cattgagggc catcaggatg
     1021 cccatgatga gcgcgaagac ggagagcggc gtgaagatgt gcaggccgat ccactcgacc
     1081 tgcgcgccga attcggcgag ttgctgcgag gtgccggcac gtgcgagccg cgtgcccatg
     1141 atgttcagca acgcgcctcc gccgacccag agtacgaccg agtagacgtg caggaacttg
     1201 aggacatcgt agccgtccac gggacgctcc cctcggcagc cggaccgcgt gtatctgagg
     1261 cggaagcata cgccgcgacg ctgtgccgtc gaggggtcca tgtgtcccgc ggcgggtccc
     1321 ccgagttagc acatcgttca cagaaggcgt ggagtcgctt cacgggcggg tgagacgatg
     1381 tcgccggaaa cggcccgcca ggctcggggg cagcatgagc aaacctcgtc atttcagtgg
     1441 cagactcgcg gcgctgagtt cggcggccat cctcgcggtg tacgccgtgg ggtacacggt
     1501 cacggaggac gccgcacaga gcgcggagtc cggaccggca ccgacgccga cgcctcgggc
     1561 gagcgctgac cccaccacga gccccacttc gacggcacgt gggacgtcgc cggcgcagac
     1621 gccaacggtc accgtgcagc gcgcgacctc gacggcgacg ccgggagcgt cgagcggcgc
     1681 gtatcgtgac gggacgtacg tgggcttcgg aacgggccgc cacggcagca tcgaggccac
     1741 ggtgacggtg gagggcggga agatcacggc ggtgcagatc agccagtgcc tgacgcgcta
     1801 cccgtgctcg cgcatcggcg cgctgcccgg gcaggtggtg agccggcagt cggcgcaggt
     1861 ggactacgtc tcgggggcga cggacagctc gcggagcttc atccaggcgg tcaccaatgc
     1921 actcgccagc gcgaactgag ccctcgacgg ggccgaggcc ggcgagcgag cggacgagcg
     1981 tgcgacggtc ggcgatgttc atggacacga cggtcacgat cgaggttgcg catcgcgacg
     2041 aggagccggc cgccctcgat gcgatcgagc g
//
LOCUS       DAORPG010000375         2066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6709_length_2066_cov_3.158130, whole genome shotgun sequence.
ACCESSION   DAORPG010000375 DAORPG010000000
VERSION     DAORPG010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6709_length_2066_cov_3.158130"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PLX85_08080"
     CDS             complement(<1..314)
                     /locus_tag="PLX85_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRC63161.1"
                     /translation="MVQKIYVGNLPFSAREGDVETLFAQYGEVLSVAVPTDRETGRVR
                     GFAFVEMSNEDAAKAIAALNGTEFGGRALNINEARPREEGGRGGGGGYGGGSRGGGGG
                     YGG"
     gene            527..1231
                     /locus_tag="PLX85_08085"
     CDS             527..1231
                     /locus_tag="PLX85_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRC63162.1"
                     /translation="MLAPIEVTHVIFDMDGVLLDTEPHYTAASTEVCRRYGREFTYAH
                     KSRMLGRRALAAADWLVAELGLPLTGEAFLLEREPILDALFATAGPMPGAFELTAHLS
                     AGGVPHAVASSSSRRNFAIKTAAHADWFARFNAVVLGDDPEVHEGKPAPDIFLVAARR
                     LGAPPEACLVVEDSPAGVAAARAAGMRVVCVPDHNIEAALFPPADEVLPSLLAFRPEA
                     WGLPPFRLSAAAEAGR"
     gene            complement(1228..1905)
                     /locus_tag="PLX85_08090"
     CDS             complement(1228..1905)
                     /locus_tag="PLX85_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC63163.1"
                     /translation="MEVANELMVRGLVVGVFQENCWVIGNRRTGEAICIDPGDEPDEI
                     LALARDMGVQIKLIANSHAHLDHILGVYGVHAQTGSKFLLHEAELDLLRNGWRDASAR
                     MGLDVEHGPPDPDGFVLEGDIVEVDGVRLQAITTPGHTPGSVSYYSEGLLFSGDTLFR
                     GSIGRTDMPGGSYEQEMDSICDRLLTLPDETLVLPGHMQETSIAAEKAQNPFVLDWMR
                     RRSAAQG"
     gene            complement(1969..>2066)
                     /locus_tag="PLX85_08095"
     CDS             complement(1969..>2066)
                     /locus_tag="PLX85_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRC63164.1"
                     /translation="FHPSQQNTFTGRLTEPMLDSVFRRAAELAAA"
BASE COUNT          289 a          816 c          623 g          338 t
ORIGIN      
        1 ccgccgtaac cgccgccacc accgcggctc ccgccgccgt agccgccccc gccgccacga
       61 ccgccctcct cacgaggacg cgcctcgttg atgttgagcg cgcgaccgcc gaactcagtg
      121 ccgttcagcg ccgcgatggc cttcgctgcg tcctcgttcg acatctcgac gaacgcgaag
      181 ccgcgcacgc ggcccgtctc gcggtcggtc gggaccgcca ccgacagcac ctcgccgtac
      241 tgcgcgaaca gcgtctcgac gtcgccttcg cgcgccgaga acgggaggtt gcctacatag
      301 atcttctgta ccaacgaatc actcctagcc ggtcgagccg acaactactg cccccgtctg
      361 cgggggctgc tgcaaaagtc tcaggctcga cgtgggcgtt cgggtgaaac gacgtggaag
      421 agatactgag aggaccgcac caacgctggt gcccttccat catacgacgc ccgagccacc
      481 ggcgtggtgg cctgcggcac aatccgcgtc gtgcgaccat acggctatgc tcgcgcccat
      541 cgaggtcacc cacgtcatct tcgacatgga cggcgtcctg ctcgacaccg agccccacta
      601 cacggccgcc tccaccgagg tctgccgccg ctacggccgc gagttcacct acgcccacaa
      661 gtcgaggatg ctcggacgac gcgccctcgc cgccgccgat tggctcgtcg ccgagctggg
      721 actgccgctg accggcgagg ccttcctcct cgaacgcgag cccatcctcg acgcgctctt
      781 tgccaccgcg gggcccatgc ccggcgcgtt cgagctgacc gctcacctct cggccggcgg
      841 cgttccacac gcggttgcct ccagttcgag ccgtcgcaac ttcgccatca agaccgccgc
      901 gcacgcggac tggttcgcgc gcttcaacgc ggttgtcctc ggcgacgacc ccgaggtgca
      961 cgagggcaag cccgctccgg acatcttcct cgtcgccgcg cgtcgcctcg gcgcgccgcc
     1021 cgaggcctgc ctggtcgtcg aggactctcc cgccggcgtc gccgccgccc gcgccgccgg
     1081 catgcgcgtt gtctgcgtcc cggaccacaa catcgaggcc gcgctcttcc ctcccgcaga
     1141 cgaggtgctc ccgagcctgc tcgccttccg ccccgaggca tggggcctgc cgcccttccg
     1201 cctcagcgca gccgccgagg ccggtcgcta gccctgggcg gccgatctcc gtcgcatcca
     1261 gtcgaggacg aacgggttct gcgccttctc ggccgcgatg ctcgtctcct gcatgtgccc
     1321 cggcagcacc agcgtctcgt ccggcagcgt cagtagccgg tcgcagatgc tgtccatctc
     1381 ctgctcgtag ctcccgcccg gcatgtcggt gcgcccgatc gatcctcgga acagcgtgtc
     1441 tccgctgaac agtaggccct cgctgtagta gctgacgctt cccggcgtat gccccggcgt
     1501 cgtgatcgcc tgcagccgca cgccgtccac ctcgacgatg tctccctcga ggacgaaccc
     1561 gtcggggtcc ggcggcccgt gctccacatc gaggcccatc cgcgccgagg cgtctcgcca
     1621 cccgtttcgc agcaggtcca gctcggcctc gtgcagcagg aacttcgaac ccgtctgcgc
     1681 gtgcaccccg tacaccccaa ggatgtggtc gaggtgggcg tgcgagttgg cgatcagctt
     1741 gatctgcacg cccatgtcgc gcgccagggc aaggatttcg tccggctcgt cgcccgggtc
     1801 gatgcagatc gcctcgcccg tgcgccggtt gccgatcacc cagcagttct cctggaacac
     1861 ccctacgacg agcccgcgca ccatcagttc gtttgcgact tccataccgc cacattccgc
     1921 gccgcgagca ggcgcgtcaa gccgcgtcgc cgtcggggga tcgtcgcctc aggccgccgc
     1981 cagctccgcc gcccgcctga acacgctgtc gagcatcggc tcggtcagcc ggcccgtaaa
     2041 cgtgttctgc tggctcgggt ggaacg
//
LOCUS       DAORPG010000376         2065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6719_length_2065_cov_3.235323, whole genome shotgun sequence.
ACCESSION   DAORPG010000376 DAORPG010000000
VERSION     DAORPG010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6719_length_2065_cov_3.235323"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..>2065)
                     /locus_tag="PLX85_08100"
     CDS             complement(236..>2065)
                     /locus_tag="PLX85_08100"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRC63165.1"
                     /translation="RRRLAEAALVAEQIEALLNEARPVWDKRLAAYRALQQSDIAILL
                     RRFANVHLFEQALEARSLDYATPSGTGFFSRQEVLDLENLLRWLAEPDDDIALVAILR
                     SPLFIVADDVLFALRQSGRALLAALHEPPAPIAPDDAARCRFAADVLTELRRRARTEA
                     AVDLLEHALTVTGFEASWAPLAGGEQALANIRKLVRIVRSLPGFSISEVAEYLLQRRE
                     DLEAREGPAILDRPDAIQIMTVHGAKGLEFPVVFVPEAHTSVRDQTPPLLWRPDLGLS
                     CTLEPEEDGEQRRRPGLYRHLRALESADERDEHVRLFYVASTRAGDYLYLSGDAGQND
                     GWLHAVEDAAAAGMLDGIELRPPVDPDAVEIVRRAAPFEVSLPEPGESYMPPLLARPP
                     VIPLRSSTPVTALRIEQAHRWRGGDAYGLIRGRITHRAIEARYGPGPVIEVPTIARQE
                     APAVSDEVRAALVADVEEMLARFGASEVASQLASPGAMPRFEAPFAWDWDGVPVHGQI
                     DLLYRLGGRWRVVDFKTDRVSASGIEGASRPYLVQLGLYARAIEAATHERPSAGLLFL
                     RLGHWYEPPWSAIDAALADARRRIDGGLVLDPELDEYLAAGDE"
BASE COUNT          305 a          687 c          776 g          297 t
ORIGIN      
        1 aggttgcccc gaggggaaac ggcaggccgg gctgcacgcg cgcggtgctc ctcgcgccaa
       61 tcaccgcagt tctccggtcg aacgcaggag gcacgagacc gggctggggt cggggctgtg
      121 ctcgctcgtt gcgcccagga catgagcgat ccagccggcg ttcatgtccg cgaactccac
      181 gccgcatagt gtacgggcgc atgcgaccac gagtccgccg aagccccgtt cctcgttact
      241 cgtcaccagc tgcgaggtac tcgtccagct ccgggtcgag caccaggccg ccatcgatgc
      301 ggcggcgtgc gtccgcgagc gccgcatcga tggccgacca tgggggctcg taccagtgcc
      361 cgagacgcag gaacaggagc ccggccgacg gtctctcgtg cgtcgccgcc tcgatcgctc
      421 gggcgtacag gccgagctgg acgaggtagg ggcgagacgc cccctcgatg cccgaggcgg
      481 agacgcgatc ggtcttgaag tcgaccacgc gccagcggcc gccgaggcgg tagagcaggt
      541 cgatctggcc atgcacgggt acgccatccc agtcccacgc gaagggagcc tcgaagcgag
      601 gcatcgcgcc gggcgacgcc agctgcgagg cgacctcgga ggctccgaag cgggccagca
      661 tctcctcgac gtcggccacg agggcggcgc gcacctcgtc gctcacggcc ggggcttcct
      721 ggcgtgcgat cgtggggacc tcgatgaccg ggccgggacc gtagcgtgcc tcgatggccc
      781 gatgggtgat gcgtccgcgg atgagtccgt aggcgtcgcc gccgcgccag cggtgcgcct
      841 gctcgatccg cagggcggtc accggggtac tgctgcgtag cgggatcacg ggcggccggg
      901 cgagcagggg cggcatgtac gactcacccg gctcggggag tgagacctcg aatggcgccg
      961 cccgacgcac gatctcgacg gcgtcggggt cgacgggtgg tcgcagctcg atgccatcga
     1021 gcatccccgc ggcagccgcg tcctcgacgg cgtgcagcca gccgtcgttc tgcccggcgt
     1081 caccggacag gtacaggtag tcgccggccc gagtcgatgc gacataaaag aggcgcacgt
     1141 gctcgtctcg ttcgtctgcg gactcgaggg cgcggaggtg gcggtacagt ccggggcggc
     1201 gtcgctgttc gccgtcctct tcgggctcga gtgtgcagga gagcccgagg tccgggcgcc
     1261 agagcagggg gggggtctga tctcggacgg atgtatgcgc ctccggtacg aagacgaccg
     1321 ggaattcgag gcccttcgcg ccatgcacgg tcatgatctg gatggcgtca ggacggtcga
     1381 ggatcgcggg cccctcgcgt gcctcgaggt cctcgcggcg ctggagcaag tactccgcga
     1441 cctcgctgat gctgaatccc ggcagggacc gcacgatgcg gaccagcttg cggatgttcg
     1501 cgagggcctg ttctccgccc gcgagtggcg cccaggatgc ctcgaagccg gtgactgtga
     1561 gcgcgtgctc gagcaggtcg accgccgctt cggtgcgcgc gcgccgtcgc agctcggtga
     1621 gcacgtctgc cgcgaagcgg cagcgcgcgg cgtcgtccgg cgcgatcggg gcgggtggct
     1681 cgtgcagggc agcgaggagc gcacggccgc tctggcgcag ggcaaacagg acgtcgtccg
     1741 cgacgatgaa caggggcgag cgcaggatcg cgacgagggc gatgtcgtcg tcgggctccg
     1801 ccagccagcg cagtagattc tcgaggtcga gcacctcctg gcggctgaag aaccccgtac
     1861 cgctcggcgt ggcgtaatcc agtgagcggg cctcgagggc ctgctcgaac aggtgcacgt
     1921 tcgcgaagcg gcgcaggagg atcgcgatgt cgctctgctg gagcgcacgg taggctgcca
     1981 gccgcttgtc ccagaccggg cgcgcttcgt tcagcagcgc ctcgatctgc tcggcgacga
     2041 gcgcggcctc cgcgagcctg cgccg
//
LOCUS       DAORPG010000377         2065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6722_length_2065_cov_2.408458, whole genome shotgun sequence.
ACCESSION   DAORPG010000377 DAORPG010000000
VERSION     DAORPG010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6722_length_2065_cov_2.408458"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..725
                     /locus_tag="PLX85_08105"
     CDS             276..725
                     /locus_tag="PLX85_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63166.1"
                     /translation="MAFALRCSDSRTVITNPTAPASTPTARDELDPEPGLAAELALLP
                     VYPNWIVNEPGAMQGTDRLGAAFFTNDSPEQVLRFFEARLESSGWAATAPAKSTTSQL
                     KLDGTAATGLSEEFTKPGFRLLVSAGPTSKSPARYQTAIGLVLDRVK"
     gene            complement(730..1185)
                     /gene="dtd"
                     /locus_tag="PLX85_08110"
     CDS             complement(730..1185)
                     /gene="dtd"
                     /locus_tag="PLX85_08110"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012938859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRC63167.1"
                     /translation="MRAVVQRVTSARVVVADEVVGAIGRGLLVYVGVARTDTEAEAAW
                     LADKLAGIRLFPGDTAEGARSMERSVLDAGGAALLVSQFTLLADTRKGRRPSFFDAAP
                     PEAAVPLLDALASALQARGIEVATGRFGAHMLVTSENDGPVTIVLDSGA"
     gene            complement(1216..1560)
                     /locus_tag="PLX85_08115"
     CDS             complement(1216..1560)
                     /locus_tag="PLX85_08115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRC63168.1"
                     /translation="MPLYDYQCEAGHRYEKRESFGAPAQQPCDQCGKPARRLLNAPPI
                     VFKGSGWYVTDSKRTLRPGADSPASSTDLPSDGGESATETAPTSNGASETATKKPARS
                     SAKKKAAASSDD"
     gene            1876..>2065
                     /gene="rpsB"
                     /locus_tag="PLX85_08120"
     CDS             1876..>2065
                     /gene="rpsB"
                     /locus_tag="PLX85_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018147174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRC63169.1"
                     /translation="MRQMLEAGVHFGHQTRRWNPHMRPYIFTERHGIHILDLAKTVPL
                     LDQALQQVRAVSSSGQQVL"
BASE COUNT          297 a          655 c          771 g          342 t
ORIGIN      
        1 gtttcatcag cgtctgggcc tgttgcgggt cgtcccagag gtctggtgcg gcggtttgtt
       61 cgcggagggt ggctagttcg tgctgtagac cgggcaggtc aaagtcgcac ctgcacatct
      121 cgcacgcgtt ccaggagctc ggcgacgcgc tgtctgagtt cgtccatagg cccccatgtt
      181 cgcacgatga ggcggatggg ggcgagtgag tacgagtgtt gtgcgagaat ccgcgcgtga
      241 aggtgatcgc cggcggagct ggtgtctgtt ttctgttggc tttcgctctg aggtgttccg
      301 actctcgtac cgtcatcacg aacccgaccg cgccggcctc gaccccgaca gcgcgcgatg
      361 aactcgaccc ggaacccggc cttgcggcgg aactggcgct gctgcccgtc taccccaatt
      421 ggattgtgaa tgagccggga gcgatgcagg ggacggatcg actgggcgcg gcattcttca
      481 ccaatgactc ccccgagcag gtgctgcgct ttttcgaggc acgccttgaa tcctcgggct
      541 gggcggcgac cgcgcccgcg aagtccacga cctcccagct gaagctcgat gggacggcgg
      601 caacggggct gtccgaggag ttcacgaagc caggctttcg actcctcgtc tcagccgggc
      661 cgacatcgaa gagccctgct cggtaccaga ccgcgatcgg cctcgtcctc gaccgagtga
      721 agtagccccc tacgctccgc tgtccagcac gatcgtcacc gggccgtcgt tctcgctcgt
      781 aacgagcatg tgggcgccga agcggcccgt ggcgacctcg atgccgcgcg cctggagggc
      841 cgaggcgagg gcgtccagga gggggacggc ggcctcgggt ggggcggcgt cgaagaagga
      901 ggggcggcgg cccttcctcg tgtcggcgag cagcgtgaac tgcgagacga gcagcgccgc
      961 gccgccggcg tcgaggacgg agcgctccat gctgcgggcg ccttcggcgg tgtcgccggg
     1021 gaagaggcgg atgccggcga gcttgtccgc gagccaggcg gcctcggcct cggtgtcggt
     1081 gcgggcgacg ccgacataca cgagcaggcc gcggccgatc gcgccgacga cctcgtcagc
     1141 cacgacgaca cgcgccgagg tgacgcgctg gaccacggcg cgcatcggag gggcggtttc
     1201 gaggagcggg cgggcctagt cgtccgagct ggcggcggcc ttcttcttgg cgctcgaccg
     1261 cgcgggcttc ttcgtcgcag tctcgctcgc gccgttcgag gtgggggccg tctcggtcgc
     1321 gctctcgccg ccgtccgagg ggaggtcggt gctggaggcg ggggagtcgg cgccggggcg
     1381 gagcgtgcgc ttgctgtcgg tgacgtacca gccggagccc ttgaagacga tcgggggggc
     1441 gttcagcagg cggcgcgcag gcttgccgca ctggtcgcag ggctgctgag cgggggcgcc
     1501 gaacgactcg cgcttctcgt agcggtgccc ggcttcgcac tggtagtcgt agagcggcac
     1561 ggcagcatcc tccacgtcgc ctgcgcgcag gtcgcgcggc ggattggtac tccgatgggg
     1621 tcggggtgtc cagtgtatgc aggcgcgagg ccattcacaa cattcgaggg gtggcgcgct
     1681 gtcgccttcg aggcgaggcg ggctatactc gaatgtcccg gacgtgcgtg gccggccgag
     1741 cgccggcgtg agactcacac ccgagcagca tccacccagg cagctatcga ggagatagcg
     1801 cagaaccgcg cccgctgtgg gccgggcatc gcccgatgag ggctgaggag gtttgtcttg
     1861 gcagcaggcg tttccatgcg gcagatgctc gaggcggggg tccacttcgg acaccagacc
     1921 cggcgctgga acccgcacat gcgaccgtac atcttcacgg agcggcacgg gatccacatc
     1981 ctggacctcg cgaagacggt gccattgctc gaccaggcgc tgcagcaggt gcgcgccgtc
     2041 tcgtcctcgg ggcagcaggt gctgt
//
LOCUS       DAORPG010000378         2062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6736_length_2062_cov_3.676134, whole genome shotgun sequence.
ACCESSION   DAORPG010000378 DAORPG010000000
VERSION     DAORPG010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6736_length_2062_cov_3.676134"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..697
                     /locus_tag="PLX85_08125"
     CDS             122..697
                     /locus_tag="PLX85_08125"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRC63170.1"
                     /translation="MNEEAPALRPALGHPGIDWSGDGGLGGPVVDREVASPEQRPVSA
                     PLRLRAVGFGVDFLVLLGIVVVAMVVVMIAAGVDTSAPQAEVERQTEDLYPVLYVVGA
                     AIQFVYNLVWNTIGWSPGKRTVGLRTVDRHGRPPGLGLGLLRTVGLMVGNVFGLGYLW
                     AFVDRDRRTIHDRIGRTWVVRARAEDVGQER"
     gene            complement(714..1655)
                     /locus_tag="PLX85_08130"
     CDS             complement(714..1655)
                     /locus_tag="PLX85_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63171.1"
                     /translation="MTLTRPQQAALAMLAGWMSARYFRTFRLAEERDRFDAVLTQRDR
                     RIGVTVGTLWDGDAPSGAGGLESLVSADLEAAGDRNAYVLWAPPGGSIPTEEPGLSDL
                     RVLVTRGVNGLEAAQRREIRRPVTLRLAKIDAAGAYMSVSGGMASVWTHLSENINGSF
                     HLDSRELLRLPEEQAEVEILTTRVRDRAVLLEPEEITRVDVHDYWLVSRVPGTEPAGL
                     TVIGSPEGADPADTGAMRRMLRGHIGRALEQRAGHGNDLSVLLLVASLAHMEDERVTA
                     ALRGMNPASYGAIDLIALVADGSVRQVLQPRSLPWES"
     gene            complement(1704..>2062)
                     /locus_tag="PLX85_08135"
     CDS             complement(1704..>2062)
                     /locus_tag="PLX85_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63172.1"
                     /translation="LFLRSFEVWHHQGTPLLGFMATNIAGAVATLAIFARFIRNWDIP
                     LILLAVLAAAVAGVIWAQQSDNDGSGFATTLKWIALVDFLLLNLALGLQVLQNGLLPV
                     LDRRDARKAAATEAEA"
BASE COUNT          290 a          744 c          730 g          298 t
ORIGIN      
        1 actgggacag tcgaggcgtg ctggtgcggg ccctcgtcag ccccgaaccc gagcacgcat
       61 ccggaccgca cgcgaacgag tcgttccgca ctctgctccg cgcgacgctc gagggcgcct
      121 cgtgaacgaa gaggccccgg cgctgcgccc tgccctcggg cacccgggga tcgactggtc
      181 gggcgatggc ggcctcggcg gcccggtcgt cgaccgcgag gttgccagcc ccgagcagcg
      241 gcccgtgtcg gcgccgctcc ggctacgcgc ggttggcttc ggcgtcgact tcctggtcct
      301 gctgggcatc gtcgtggtcg cgatggtggt cgtgatgatc gccgccggcg tcgacaccag
      361 cgcgccgcag gccgaggtcg agcgtcagac cgaggacctc taccccgtcc tctacgttgt
      421 gggagcggcg atccagttcg tctacaacct cgtctggaac accatcggtt ggtccccggg
      481 gaagcgcacc gtggggctgc gtacggtcga ccggcacggc cggccgcccg gcctcggcct
      541 cgggctgctg cgcacggtcg gcctgatggt cgggaacgtg ttcggactcg gctacctctg
      601 ggcgttcgtc gaccgcgacc gccgcaccat ccacgaccgc attggtcgca cctgggtggt
      661 gcgcgcgcgt gccgaggacg tcgggcagga gcgttagccc accgcgctcg cggctacgac
      721 tcccagggca gcgatcgagg ctgcagcacc tgtcgcacgg atccgtccgc gacgagcgcg
      781 atgaggtcga tcgcgccata cgaggccggg ttcatccccc gcagggcggc cgtcacccgc
      841 tcgtcctcca tatgcgcaag cgaggcgacc aacagcagca cgctcaggtc attgccgtgg
      901 cctgcccgct gctccagtgc ccgcccgatg tgaccgcgca gcatccgccg catggcgccg
      961 gtgtcggccg ggtcggcgcc ctcgggcgat ccgatcacgg tcagtccggc gggctcggtg
     1021 cccggcaccc gggagaccag ccagtagtcg tgcacgtcga cgcgcgtgat ttcctccggc
     1081 tcgagcagca ccgcacggtc tcgcacccgt gtcgtgagga tctccacctc ggcctgctcc
     1141 tcggggagcc gcagcagctc gcgagagtcg aggtgaaacg aaccgttgat gttctcggag
     1201 agatgcgtcc agaccgaggc catgccgccg ctgaccgaca tgtacgcgcc ggccgcgtcg
     1261 atctttgcga gccgcagggt gaccggccgc cggatctcgc ggcgctgggc ggcctcgagc
     1321 ccgttgaccc cgcgcgtgac caggacgcgt aggtcggaga ggccgggctc ctcggtcggt
     1381 atcgatccgc ccgggggcgc ccagagcacg tacgcgttgc ggtcgcccgc cgcctccaga
     1441 tcggcgctga ccagcgactc gaggccgccg gcgccggagg gggcgtcccc atcccacagc
     1501 gtccccacgg tgacgccgat acgccggtcg cgctgcgtga gcaccgcatc gaagcggtcg
     1561 cgctcctcgg ccaggcggaa cgtgcggaaa tagcgcgcgc tcatccaacc cgcgagcatc
     1621 gcgagcgccg cctgctgcgg acgtgtgagg gtcaatgcgg gccggtcggt ttccgcggcg
     1681 cttcctcgtg agccggagcg gcgctaggcc tcggcctcgg tggccgccgc cttgcgggcg
     1741 tcacgccggt cgaggaccgg caacagcccg ttctgcagca cctgcagtcc cagcgcgagg
     1801 ttcagcagca ggaagtcgac gagcgcgatc cacttcagcg tcgtggcgaa gccggagccg
     1861 tcgttgtcgg actgctgcgc ccagatgacg cccgcaaccg ccgccgccag gaccgcgagc
     1921 aggatcagcg ggatgtccca gttgcggatg aagcgcgcga agatcgccag cgtcgccacg
     1981 gcgccggcga tgttggtcgc catgaacccc agtagcggcg tcccctggtg gtgccacacc
     2041 tcgaaggagc gcaggaagag ca
//
LOCUS       DAORPG010000379         2059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6765_length_2059_cov_2.437126, whole genome shotgun sequence.
ACCESSION   DAORPG010000379 DAORPG010000000
VERSION     DAORPG010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6765_length_2059_cov_2.437126"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..1623
                     /locus_tag="PLX85_08140"
     CDS             343..1623
                     /locus_tag="PLX85_08140"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRC63173.1"
                     /translation="MGGPLAGIKVLEITQIVAGPYCGMNLADLGAEVIKIEPPEGEGM
                     RRIGGFAPGESKGFHTLNRGKRSLVLDLKRPEAQELVHQIIPQFDVFVINSRAGVAKR
                     LRLDYDTLRQFRPDLVYLDSTGYGAEGPSATRAGSDIVAQGYSGLMAGEGKLDEWGAP
                     KSISCTAVADYATGLAAAMGICAALYRRSVTGEGEYLQTSLLQSALSIQGASVARLPV
                     SDELLRRATLDAIAEARARGASYEEIINIRNGSNAATGQAFRLYYNGYRAKDGAVILG
                     ALTPANQEQMRRAMGITDDPSADPNFNALDPDSIRAVEEMGERIRQIMLTKTMDEWVA
                     LFDEEGAPISKVSLPEDLPDDPQVQAMGYMLEVEHEATGPELNVGPIVFARNNPTGSP
                     LPAPVLGGHSDEVLSELGLDGDQVASLRAVGAIG"
     gene            1727..>2059
                     /locus_tag="PLX85_08145"
     CDS             1727..>2059
                     /locus_tag="PLX85_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63174.1"
                     /translation="MSAATSDSAEGRTAASPAGSVTAPSAASERPRFLRTFESLGDAN
                     YRWFFGAMVAGFTGLSMQTFVAGWLTYELTGSFAALGFMSLANGLSNIVFSNIGGVMA
                     DRVARKQSL"
BASE COUNT          322 a          632 c          784 g          321 t
ORIGIN      
        1 agcctcgcac ttgcgggcgg cggccaggcg cgagcggcga attaaccgcc gcgcggggcg
       61 ctgacccgtc accccttgat cccctctccc acttcgcggg cgaggggaag gacgcccgtc
      121 tctcgcaaag tgtagtgatc gagatgttca gccccggagt ggcggcgagc agcctctccc
      181 ctccacctcg ccacggggca gaagcgtagc ggggagcgcc tgcccgcggc ggcgtccccc
      241 tgcccggccg tttgtgtagt agggccgaag tgttgcctct cggccgggga ggctgtacca
      301 caccggcaac ggaaatgcgt cgacgctgag gggagcgcag ctgtgggtgg gccactggcc
      361 ggaatcaagg tgctggagat cacgcagatc gtggcggggc cgtactgcgg gatgaacctc
      421 gcggacctcg gggcggaggt catcaagatc gagccacccg agggtgaggg gatgcgacgc
      481 atcggcgggt tcgcgccggg tgagagcaag ggcttccaca cgctgaaccg cgggaagcgc
      541 agcctggtgc tcgacctgaa gcgcccggag gcgcaggagc tggtgcacca gatcattccg
      601 cagtttgacg tgttcgtgat caactcgcgg gcgggggtgg cgaagcggct gcggctcgac
      661 tacgacacgc tgcgtcagtt ccggccggac ctcgtgtacc tcgacagcac cggctacggg
      721 gcggaggggc cgagcgcgac gcgcgcgggt tcggacatcg tcgcgcaggg atattcaggg
      781 ctgatggcgg gcgaggggaa gctcgacgag tggggcgcgc cgaagtcgat ctcgtgcacg
      841 gcggtggccg actacgcgac cgggctcgcg gccgcaatgg gcatctgtgc ggcgctgtac
      901 cgccggtctg tgacaggcga gggcgagtac ctgcagacct cgctgctgca gtcggcgctg
      961 tcgatccagg gggcttcggt ggcgcggctg cccgtgtcgg atgagttgct gcgaagggcg
     1021 acgctcgacg cgatcgcgga ggcccgggcg cgtggggcct cgtacgagga gatcatcaac
     1081 atccggaacg gcagcaacgc ggcgaccggg caggcgttcc ggctgtacta caacggatac
     1141 cgggcaaagg atggggcagt catcctcggg gctctcacgc cggcgaacca ggagcagatg
     1201 cggcgcgcga tggggattac cgacgaccca agcgctgacc cgaacttcaa cgcgctcgac
     1261 ccggattcga tccgggccgt ggaagagatg ggcgagcgga tccgtcaaat catgctgacg
     1321 aagacgatgg atgagtgggt ggcgctcttc gatgaggaag gggctcccat ctcgaaggtg
     1381 agcctgcccg aggacctgcc ggacgacccc caggtgcaag cgatggggta catgcttgag
     1441 gtcgagcacg aggcgacggg cccggagctg aacgtggggc cgattgtgtt cgcgcggaac
     1501 aacccgacgg gatcgccgct gccggcgccc gtgctcggtg ggcacagtga cgaggtgctg
     1561 tctgagctcg ggctcgacgg cgatcaggtg gcgtcgttgc gggcggtggg cgcgatcggg
     1621 tagggcctcg atcgttccgc atttcgtcga gggcggccgt gtggccgccc tcaccgcttc
     1681 gcaggcgcgt atcgcgcgag cgtgcgaggc cgtagagtcg cccgtcatga gcgcagcaac
     1741 gtccgactcg gcggaaggcc gcaccgccgc ctcccctgcc gggagtgtga ccgcgccctc
     1801 cgccgcctcg gagcggccgc ggttcctgcg gacgttcgaa tcgctgggcg acgccaacta
     1861 ccgctggttc ttcggggcga tggtggcggg gttcaccggg ctctcgatgc agacgttcgt
     1921 ggccgggtgg ctgacctacg agctgacggg ctcgttcgcc gcgcttggtt ttatgtcgct
     1981 cgccaacggg ctgtcgaaca ttgttttctc gaacatcggc ggcgtgatgg cggaccgcgt
     2041 ggcgcggaag cagtcgctc
//
LOCUS       DAORPG010000380         2051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6800_length_2051_cov_2.971443, whole genome shotgun sequence.
ACCESSION   DAORPG010000380 DAORPG010000000
VERSION     DAORPG010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6800_length_2051_cov_2.971443"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..1655)
                     /locus_tag="PLX85_08150"
     CDS             complement(45..1655)
                     /locus_tag="PLX85_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63175.1"
                     /translation="MAAETEAGSRLAVGRAPTPRAAARSGAGLGLRTYLTGSGVWPLA
                     VALSVVAVLLPVALTEFAVADDAAMLWMAVSGESTAQFGDSVIAQNAVGGRPLAGLLV
                     QVVFHVAGTIDHLRYVRALSVASVALFALLLHWNLMRAGLDRVVAVLIAVLVCSMPGF
                     LVFASWGVLIAAPLAAIAAGVASMVTVRGVDRGIGLRSWEYPTAAGLVLAGTWLYQPP
                     AMFYWVFLAIAIVGALDDPVRAWRLAWTHLLVGAVGLAVSFASVKAFVWYFGSDTPGA
                     TRSGLSHDVPGRLRWFGEEPLYHALNLFDLTPTQWEAAIVVAVAAAGMLLWLFTSVRR
                     PWLYAGIAIALVPLSHLPNLVVTDPWSPLRTQTALAGLLALYLSLGAIGLWLVADRAT
                     GLRLNEDERRLWRILPRGVAVAVVAVSCVVGTSNVSNLNARPLNTELRMLRGQVRGLA
                     PETPRLTFVFTDWYGGMTDFVAYDELGLPFGARPWAPGPMIDLLLDEEGRLTSGYRGP
                     DIDYYMSGSTRFPPGQPVIDLRGIRALR"
     gene            complement(1734..>2051)
                     /locus_tag="PLX85_08155"
     CDS             complement(1734..>2051)
                     /locus_tag="PLX85_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63176.1"
                     /translation="GTGSTARSLQLGGLEGVGPVLPPGDGLASLVGPTPEGPGVATLQ
                     YRGPDQFPARFSHLLLGGRSPSAFYRANAAGRFEAFIVGAPRVADTFEVFRDGDIVLA
                     VYR"
BASE COUNT          359 a          839 c          616 g          237 t
ORIGIN      
        1 cacggccgcc gccgcggtgt gacgctgcgc tgcggcgctc cacctcagcg cagcgcgcga
       61 atcccccgca ggtcgatcac cggctgcccc ggcggaaacc gtgtcgatcc cgacatgtag
      121 taatcgatgt ccggcccccg gtaccccgag gtcagtcggc cctcctcgtc gagcagcagg
      181 tcgatcatcg gcccgggcgc ccacgggcgc gccccgaacg gtaaccccag ctcgtcgtac
      241 gccacgaagt cggtcatgcc cccgtaccag tccgtgaaca cgaaggtgag gcgaggcgtc
      301 tcgggtgcga gcccccgtac ctgcccgcgc agcatccgca gctcggtgtt gagaggccgc
      361 gcgttcaggt tcgacacgtt cgaggtcccc acgacgcagc tcaccgccac aactgccacc
      421 gccacccctc gaggcaggat ccgccacagc cgtcgctcgt cctcgttgag ccgtagccca
      481 gttgcccggt cggcgaccag ccacagcccg atcgccccca ggctcaggta gagcgccagc
      541 agtccggcca gcgccgtctg cgtgcgcagc ggcgaccacg gatcggtcac cacgaggttg
      601 gggagatggc tcagcggcac cagcgcgatc gcgattcccg cgtacagcca gggccggcgc
      661 acgctcgtga acagccagag cagcatcccg gcggcggcga ccgcgacgac gatcgcggcc
      721 tcccactgcg tcggcgtcag gtcgaatagg ttcagcgcgt ggtagagcgg ctcctcgccg
      781 aaccaccgca gccgtcccgg cacgtcatgg ctcaacccgc tgcgtgttgc ccccggcgtg
      841 tccgacccga agtaccacac gaacgctttc acgctcgcga acgagaccgc cagccccacc
      901 gccccgacca gcaggtgcgt ccacgccagc cgccacgccc gcaccggatc atcgagggcg
      961 cccacgatcg cgatcgccag gaacacccag tagaacatcg ccggcggctg gtacagccac
     1021 gtgccggcca gcaccaggcc tgcggccgtc ggatactccc atgaccgcag cccgatcccc
     1081 cggtcgaccc cacgcacggt caccatcgag gccacgcccg ccgcgatcgc cgccagcgga
     1141 gcggcgatca gcacgcccca cgacgcaaac accagaaacc ccggcatcga gcacaccagc
     1201 accgcgatca gcacagccac gacacggtcc aggccggctc gcatcagatt ccagtgcagc
     1261 agcagcgcga acagcgcgac gctcgccacg ctcagagccc gtacgtatcg caggtggtca
     1321 atcgtcccgg cgacgtggaa caccacctgc accagcaacc ccgcgagcgg tcgacctccg
     1381 accgcgtttt gcgcgatgac gctgtcgccg aattgcgccg tcgactcccc gctgaccgcc
     1441 atccacagca ttgcggcgtc gtccgccacc gcgaactcgg tgagcgcgac cggcagcagc
     1501 accgccacca ccgaaagcgc caccgccagc ggccataccc ccgacccggt gaggtacgtt
     1561 cgcagcccga gccccgcacc gctccgcgcg gccgctcgag gtgtcggcgc tcgcccaaca
     1621 gcgagccggg accccgcctc cgtctccgcc gccatgcccc atcctccggc acgccggcaa
     1681 catcacgagc gcgcgacggt catcgtaagt cctgtttaag ggcgtggcga agcctagcgg
     1741 tacaccgcca gcacgatgtc cccgtcacgg aacacctcga acgtatccgc cacccggggt
     1801 gcgcccacaa tgaacgcctc gaagcgcccc gccgcgttcg cgcggtagaa cgccgacggc
     1861 gatcgcccgc ccagcaacag atgcgagaac cgtgccggga actggtccgg cccccggtac
     1921 tgcagcgtcg ccacaccggg cccctccggg gtcggcccca cgaggctcgc cagtccgtcg
     1981 cccggaggca gcaccggccc gaccccctcg agccccccga gctgcaacga ccgcgccgtc
     2041 gaaccggtgc c
//
LOCUS       DAORPG010000381         2049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6809_length_2049_cov_3.857573, whole genome shotgun sequence.
ACCESSION   DAORPG010000381 DAORPG010000000
VERSION     DAORPG010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6809_length_2049_cov_3.857573"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..1470
                     /locus_tag="PLX85_08160"
     CDS             244..1470
                     /locus_tag="PLX85_08160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HRC63177.1"
                     /translation="MRAEIISIGTEIMLGEITDTNAAFIASKLPQFGIDLLYVTQVGD
                     DVARLQEVIARAWQRSDLVFTTGGLGPTEDDLTRECVAAVLGEPMTVDAEQEAHLRQW
                     FAQRTREFPERNLKQATTIPSCRVLPNPRGTAPGWWVERDGHVIVVMPGPPAEMTHMW
                     EREVDPELERRSTAVLVSRTLKTSGLGEGSVDERLGDLWKSANPYLGIYARRDGVHVR
                     ISAKAPTREEAWALIVPVEQEVRRILGPAIWGVDDDSLAAGVGKLLRDAGLTLGVMES
                     ATGGAIADAITDGEGSTEYFLGSLVTYATQAKIDLGVPGGVVFTHGVISEATAEAMAR
                     AARERLGSDVGLGITGIAGGAEMEGQPPGTMHIAVTDGDRMLYRQYAYYQGREAAKRR
                     AVTESLNLLRSFLQSE"
     gene            1478..>2049
                     /locus_tag="PLX85_08165"
     CDS             1478..>2049
                     /locus_tag="PLX85_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRC63178.1"
                     /translation="MTGQELPTRKLGRTGLEVTKLGYGAMSLDDRFGRVVSPDEANRI
                     LNAVLDAGINYIDTSPDYGPSEEMIGQAIADRRGEYFLATKCGCPVAASAGTGHAYTP
                     ENIRAAVERSLRRMRTNYLDVVQFHGNPSMAMLDEQGAVEVLRDLQRAGAIRFIGMSG
                     AGDRLDAQVASGVFDVFQIPYSALQREHEGA"
BASE COUNT          346 a          622 c          787 g          294 t
ORIGIN      
        1 tcgcccagca tgatttccgt gccgatcgag atgatttccg cctgcattgc tcgctccccc
       61 gtgcgctgac ctggcgatgg tagcagagcg cggtcccgct cccgccccgg ccagccgagg
      121 tgccagacac gaaggagggg agcatgtgct cccctccttc gtgtctggca cctcggttgg
      181 ccggggcggg agcgggaccg cgctctgcta ccatcgcgag gtcagcggac gggggagcga
      241 gcaatgcggg cggaaatcat ctcgatcggc acggaaatca tgctgggcga gatcacggac
      301 acgaacgcgg cgttcatcgc ctcgaaactc ccgcagttcg ggatcgacct gctgtacgtg
      361 acgcaggtgg gcgacgacgt ggcacggctg caggaagtga tcgcgcgggc gtggcagcgc
      421 tccgacctgg tgttcacgac gggcgggctc ggaccgaccg aggacgatct gacgcgcgaa
      481 tgcgtcgcgg ccgtgctcgg cgagccgatg accgtcgacg cggagcagga ggcgcatcta
      541 cggcagtggt tcgcgcagcg gacacgtgag tttcccgagc gcaacctgaa gcaggcgaca
      601 acgattccct cgtgccgggt gctgccgaac ccacgaggaa cagcccctgg ctggtgggtc
      661 gagcgtgacg gccacgtgat cgtggtgatg ccagggccgc cggccgagat gacgcatatg
      721 tgggagcgcg aggtcgaccc ggagctcgaa cgacgctcga cggccgtcct ggtgtcgcgg
      781 acgctgaaga ccagcggcct gggcgagggc tcggtagacg aacggctggg cgacctctgg
      841 aagagcgcga acccatacct cggaatctac gcgcggcggg acggggtgca tgtgcgaatc
      901 agcgccaagg cgccgacgcg ggaggaggcg tgggcgctca tcgtgccggt ggagcaagag
      961 gtgcgccgca tcctcgggcc ggcgatctgg ggcgttgacg acgactcgct ggcggcgggg
     1021 gtcggcaagc tcctgcgcga cgcggggttg accctcggcg tcatggagtc agcgacgggc
     1081 ggagcgatcg ccgatgcgat cacagacggc gaaggatcga cggagtactt cctcggcagc
     1141 ctcgtgacgt acgcgacgca ggcgaagatc gacctcggcg tgccaggggg cgtcgtgttc
     1201 acgcatggcg tgatctcgga ggccacggcc gaggcgatgg cgcgcgcggc gcgggagcgg
     1261 ctgggctccg acgtcggcct cgggatcacg gggattgccg gcggcgccga gatggaggga
     1321 cagccgcccg ggacgatgca catcgcggtg acggatgggg accggatgct gtaccgccag
     1381 tatgcgtact accagggccg cgaagcggcg aagcggcggg cggtgacgga gtcgctcaac
     1441 ctgctgcggt cgttcctgca gtcggagtga ggcgaccatg acgggccagg agctgccgac
     1501 acggaagctg ggacggaccg gcctcgaagt cacgaagctg ggctacgggg cgatgtcgct
     1561 cgacgatcga ttcgggcgtg tcgtctcgcc ggacgaggca aaccgcatcc tgaacgcggt
     1621 gctcgatgcc ggcatcaact acatcgacac gtcgccagac tacgggccga gcgaggagat
     1681 gatcgggcag gcgatcgccg atcggcgcgg ggagtacttc ctcgcgacga agtgcggttg
     1741 cccggtggca gcctcggcgg gcacggggca cgcgtacacg ccggagaaca tcagggcggc
     1801 ggtcgaacgg agtctgcgcc ggatgcgcac gaactacctc gacgtcgtgc agtttcacgg
     1861 gaacccgtcg atggcgatgc tcgacgagca gggcgccgtc gaggtgctgc gcgacctcca
     1921 gcgggcgggc gcgatccggt tcatcgggat gtcgggggca ggggaccgcc tcgacgcgca
     1981 ggtcgcctca ggcgtcttcg acgtgtttca gattccgtat tcggcactgc agcgggagca
     2041 tgagggggc
//
LOCUS       DAORPG010000382         2047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6818_length_2047_cov_3.110442, whole genome shotgun sequence.
ACCESSION   DAORPG010000382 DAORPG010000000
VERSION     DAORPG010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6818_length_2047_cov_3.110442"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /gene="rsmD"
                     /locus_tag="PLX85_08170"
     CDS             <1..617
                     /gene="rsmD"
                     /locus_tag="PLX85_08170"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRC63179.1"
                     /translation="PPPQRRPGRRAERRSLEAEQRMRVIAGSARGIVLRQPRGSGTRP
                     TSGRLRESLFAMLEAAGADFSAALDLYAGTGALGIEALSRGNGRCTFIDTDARAIEAI
                     RENLRHVGFEDRATVVPARVGRWRPPEDARYTLVTADPPYDDGQAWAAIAATVERVLA
                     PHAIVAVEHSARLPSPEVLAGLSRWRERRQGDGAVALYRAAESG"
     gene            655..1131
                     /gene="coaD"
                     /locus_tag="PLX85_08175"
     CDS             655..1131
                     /gene="coaD"
                     /locus_tag="PLX85_08175"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRC63180.1"
                     /translation="MTVAMYPGRFDPVTNGHLDIVRRAVSIYEGVVVAVADSRSTLFT
                     TEERVALFSAAVADMPTVRVIANKGLTVDAARDEGAKVLVKGLRAITDFNSEFDMALM
                     NRKMAPEVESTFLMTSVEYLFVSASRIRELASFGYPIEDMVPPVVAEALREKFKNR"
     gene            1143..1964
                     /locus_tag="PLX85_08180"
     CDS             1143..1964
                     /locus_tag="PLX85_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63181.1"
                     /translation="MDLLHLVDRLEELVGSAQKMPIGSRAIVDRRRILDIVDQMRVAV
                     PQEVREAQEIVNNRDEIFREAEEEARLIVARAEERASRLVEQHELTIAARTRAEEIAR
                     ETEARIEERVAEANDEIQGRVQDSRRVADQQMEAADEYARELLTRLEGQLQAFVRSVQ
                     SGISQLQQPSATAPAAQPASDPAAFDYSEEESAIAIDEPVDVDPDDTEDGLEDLLQRP
                     ALDELPDPPPAEPGVIDDFAMPQLDDEPAAGSTWARPATAGHDDPDEEPEHPRGA"
BASE COUNT          336 a          780 c          654 g          277 t
ORIGIN      
        1 taccgccacc gcaacggcga ccgggacgcc gcgctgagcg acggagcctc gaggccgagc
       61 agcgcatgcg cgtgatcgcc ggctcggcgc gcggcatcgt gctgcgccag cctagaggct
      121 ccggcacacg tccgacctcc ggccggctgc gcgagtccct cttcgcgatg cttgaggcgg
      181 ctggcgctga tttcagcgcc gccctcgatc tctacgccgg caccggcgcg ctcggcatcg
      241 aggccctctc acgcggcaac gggcgctgca cgttcatcga cacggatgcg agagcgatcg
      301 aggccatccg agagaacctg cgccatgtgg ggttcgaaga ccgcgccacc gtcgtccccg
      361 ctcgcgtcgg ccgctggcgc ccgccggagg atgcccggta cacgctcgta accgccgatc
      421 cgccgtatga tgacggccag gcttgggcag cgatcgcggc cacggtggag cgcgtcctcg
      481 cgccgcacgc catcgtcgcc gtcgagcatt ccgctcggtt gccgtcgccc gaagtcctgg
      541 ccggcctcag ccgctggcgt gaacgccggc agggcgatgg ggccgtcgcg ttgtaccgag
      601 cagcagaatc gggctgaggc gccagccagt agcagtcagt aggggggaca ctgcatgacc
      661 gtcgcgatgt acccgggacg ctttgacccc gtcaccaacg ggcacctcga tatcgtccgc
      721 cgcgcggtca gcatctacga gggcgtcgtc gtcgccgtag ccgacagcag gtccaccctc
      781 ttcaccaccg aagaacgagt cgccctcttc tcagccgccg tcgccgacat gccgacggtc
      841 cgcgtgatcg cgaacaaggg cctcactgtc gacgcagccc gcgacgaggg cgccaaggtg
      901 cttgtaaagg gtctgcgcgc catcacggac ttcaactccg agttcgacat ggcgctcatg
      961 aaccgcaaga tggcccccga ggtcgaatcg accttcctca tgacctcggt cgagtacctc
     1021 ttcgtcagtg cctctcgcat ccgcgagctg gccagcttcg gctacccgat cgaggacatg
     1081 gtgcccccgg tggtcgcgga agccctccgc gagaagttca agaaccgcta gcggaggacg
     1141 acatggacct gctgcacttg gtcgaccgcc tcgaggagct cgtcggcagc gcccagaaga
     1201 tgcccatcgg cagtcgcgcc atcgtcgacc gccgccgcat cctcgacatc gtcgaccaga
     1261 tgcgcgtcgc cgtgccccag gaggtccgcg aggcccagga gatcgtcaac aaccgcgacg
     1321 agatcttccg tgaggccgag gaagaagctc gcctcatcgt cgcccgcgcc gaggaacgcg
     1381 cctcccgcct ggttgagcag cacgagctga ccatcgccgc ccgtacccgc gccgaggaga
     1441 tcgcgcgcga gaccgaggcc cgcatcgagg agcgcgtcgc cgaggccaac gacgagatcc
     1501 aggggcgcgt gcaggactca cgccgcgtcg ccgaccagca gatggaagcc gccgacgagt
     1561 acgcccgcga gctccttacc cgcctcgagg gccagctaca ggccttcgta cgctccgtcc
     1621 agtccggcat ctcacagctg cagcagccgt cggcgaccgc gccggccgcg cagccggcct
     1681 ccgatcccgc ggccttcgac tactccgagg aggagtccgc catcgccatc gatgagccgg
     1741 tcgacgtcga cccggacgac accgaggacg gcctcgaaga cctcctccag cgtccggccc
     1801 tcgacgagct gccggacccg cccccggccg agcccggcgt catcgatgac ttcgcgatgc
     1861 cccagctcga cgacgagccc gcggctggct ccacctgggc ccgccccgcc accgccgggc
     1921 atgacgaccc cgacgaggag cccgagcacc ctcgaggcgc gtagccgggc cgcgacccgt
     1981 ctacttcagc acgatgttga gcagccgccc cggcacgtag accacccgcg ccacctcgcg
     2041 cccctcg
//
LOCUS       DAORPG010000383         2039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6868_length_2039_cov_3.066028, whole genome shotgun sequence.
ACCESSION   DAORPG010000383 DAORPG010000000
VERSION     DAORPG010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6868_length_2039_cov_3.066028"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1075)
                     /locus_tag="PLX85_08185"
     CDS             complement(80..1075)
                     /locus_tag="PLX85_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HRC63182.1"
                     /translation="MAMLAAVGAVLLVAAACGGGDDEVLTIYAGRSQNLVQPLLEQFA
                     KDTGVHIRVKYGDSTDLALGILEEGKNSPADVYYAQDIGALGALKAAGRLQPLSEATL
                     NQVPAAFRSPDGQWVGVSGRARVIVYNTDDVDPKSLPSSILDYTDSKWRGQIAIVPRS
                     DGFPEFVTALRLLKGEEFARTWLRALKANDPKTYPNNLAAIQAVANGEVKVAFLNHYY
                     LYRFLEEEGQGFKARNSYFGGGDLGGLFLVSGAAILDTSKHREAAERFINYLLTQSSQ
                     QYFAEQTFEYPLVAGVPTDPGLPPLASMKPPEIDLSNLTDLQGSLALMRDTGILP"
     gene            complement(1134..1676)
                     /locus_tag="PLX85_08190"
     CDS             complement(1134..1676)
                     /locus_tag="PLX85_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63183.1"
                     /translation="MRKWMAAPGLAACLALVALAAACGGEDRPKVDVIEDGGTSSASA
                     SGPGTAAPGEVSPKPAGAQQVDVVLREFALEPAVASVKAGEIYFLADNRGPADAHELV
                     IVKTDLAPDKLPVEKGKVSESKVKVVDEIEAFAAKSQASKSVKLSAGTYVLLCNIVET
                     DDGQTESHYELGMRAALRVE"
     gene            complement(1693..>2039)
                     /locus_tag="PLX85_08195"
     CDS             complement(1693..>2039)
                     /locus_tag="PLX85_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC63184.1"
                     /translation="YVLGSRFRGEIEGMSRSRRAANHGFSLLLSLLCGRRISDGQTGY
                     RAFSRRALEVAEIVHDYNYAQVLTLDLLKKGMRMAEAPVRYRRRTSGASFVRAEYLWR
                     VPVGIAREMLGS"
BASE COUNT          295 a          734 c          676 g          334 t
ORIGIN      
        1 gccgtgaccg ccgtcgccag cgcggcggtc gtccacggca ggcgccgcgc ccgacctcga
       61 gcgtgcgtaa gcaccgccat cagggcagga tcccggtgtc ccgcatcagg gcgagcgagc
      121 cctgcaggtc ggtgaggttg ctcaggtcga tctccggcgg cttcatgctc gcgagcggcg
      181 gcagcccggg gtcggttggc acgcccgcga ccagcgggta ctcgaaggtc tgctccgcga
      241 agtactgctg cgagctctgc gtcagcaggt agttgatgaa gcgctcggcc gcctcgcggt
      301 gcttcgaggt gtcgaggatc gccgcgcctg acaccaggaa cagcccgccg aggtcaccgc
      361 ctccgaagta ggagttgcgg gctttgaacc cctggccctc ctcctcgagg aaccggtaga
      421 ggtagtagtg gttgaggaac gcgaccttca cctcgccgtt tgcgaccgcc tggatggcgg
      481 ccaggttgtt tgggtacgtc ttcgggtcat tcgccttcag cgcgcgcagc caggtgcggg
      541 cgaactcctc gcccttgagc agccgcagcg cggtcacgaa ctcggggaag ccgtcgcttc
      601 gcggcacgat cgcgatctgg ccgcgccact tgctgtcggt gtagtcgagg atcgaggacg
      661 gcagcgactt cgggtcaacg tcgtccgtgt tgtagacgat gacgcgcgcc cggccggaga
      721 cgcccaccca ctggccgtcc ggtgagcgga acgcggccgg cacctggttc agggtcgcct
      781 cggagagcgg ctggaggcgt ccggcggcct tgagggcccc cagcgccccg atgtcctgcg
      841 cgtagtagac gtctgcaggc gagttcttcc cctcctcgag gatgccgagc gccaggtccg
      901 tgctgtcgcc gtacttcacg cggatatgta cgccggtgtc cttcgcgaac tgctcgagca
      961 agggctgcac gaggttctgt gaccgtccgg cgtagatcgt cagcacctcg tcgtccccgc
     1021 cgccgcaggc agccgcgacg agcaggacgg cgccgaccgc cgccagcatc gccatcagtc
     1081 gaagcgtccg cctcacgctc gtgcctttcc cgtcacgcgc ctcggccccc ccgctattcg
     1141 acgcggaggg cggcgcgcat ccccagctcg tagtggctct cggtctgacc gtcgtccgtt
     1201 tcgacgatat tgcagagcag gacatacgtc cccgccgaca gcttcacgct cttgctggcc
     1261 tgcgacttcg cggcgaacgc ctcgatttca tcgacgacct tcaccttgct ctccgagacc
     1321 ttgcccttct cgaccgggag cttgtcgggc gccaggtcgg tcttcacgat gaccagctcg
     1381 tgcgcgtcgg cgggcccccg gttgtcggcg aggaagtaga tctcgcctgc cttcacgctc
     1441 gccacggcgg gctcgagggc gaactcgcgg agcacgacgt ccacctgctg cgccccggcc
     1501 ggcttcggcg agacctctcc gggtgcggcg gtcccgggcc cgctggcgct ggccgaggaa
     1561 gtgccgccgt cctcgatcac gtcgactttc gggcggtcct cgccgccgca ggccgcggcg
     1621 agcgccacca gcgcaaggca ggccgccagc cccggggccg ccatccactt ccgcatgccg
     1681 atactcctca cctcagctcc cgagcatctc gcgcgcgatc ccgaccggga cgcgccagag
     1741 gtactcggca cgaacgaacg aggcgccact ggtccgccgc cggtagcgca cgggggcctc
     1801 ggccatgcgc atccctttct tgagcaggtc aagcgtcagc acctgcgcgt agttgtagtc
     1861 gtggacgatc tccgcgacct cgagcgcgcg cctcgagaac gcgcgatagc ccgtttgccc
     1921 gtccgagatg cgccgcccgc acaacagcga gagcaacaga ctgaagccgt gattcgccgc
     1981 ccgccgcgac cgcgacatcc cctcgatttc cccgcgaaag cgggagccga ggacgtagt
//
LOCUS       DAORPG010000384         2036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6887_length_2036_cov_2.561837, whole genome shotgun sequence.
ACCESSION   DAORPG010000384 DAORPG010000000
VERSION     DAORPG010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6887_length_2036_cov_2.561837"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PLX85_08200"
     CDS             <1..293
                     /locus_tag="PLX85_08200"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRC63185.1"
                     /translation="DDWQSLVAGTAIFAIVNTLLKPLATMLSCCLILATFGLFVVVIN
                     AAMLGITAWAAGQLELNFHVDGFWSAVLGALVISAVAMVATWLIRPPRVQRF"
     gene            complement(330..887)
                     /locus_tag="PLX85_08205"
     CDS             complement(330..887)
                     /locus_tag="PLX85_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRC63186.1"
                     /translation="MDTGTGNGPDAMAGRTTVERTSDRELVVTRTFNAPARFVFEAWT
                     RAELFQRWWVPKSFGLTLLSCELDVRVGGGYRLLFSHPAAPEPVAFHGSYLEVQPHSR
                     LVWSNDEAGGSGQVTTVTFEEHAGQTLLVMRDLYPSKEALDEAIASGSAAASPGDQSG
                     IEETFAQLDQLLDADGGESTAAPSS"
     gene            complement(892..1227)
                     /locus_tag="PLX85_08210"
     CDS             complement(892..1227)
                     /locus_tag="PLX85_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRC63187.1"
                     /translation="MVQYLTRLDASFGALSDATRRGVLEQLGRADASITDLAVKFRMT
                     RTGIKKHVAVLEQAGLVTTEKVGRVRTCRLGPRRLEEEATWIEGYRQLWEARFDELDR
                     VVEELRRRE"
     gene            complement(1357..>2036)
                     /locus_tag="PLX85_08215"
     CDS             complement(1357..>2036)
                     /locus_tag="PLX85_08215"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HRC63188.1"
                     /translation="SLAPDATAVAELIQAAGLKATDLAAEVGRAGATLEAARAQVDQA
                     TRLLEDARSYRRTSDLASELGLELQANRFVAYIQREAMHVLAADAGERMLQLSRDRYR
                     LEADGDEFVVVDRLNGDERRSVRTLSGGETFLASLALALALSERLPELSGHGGALSLD
                     SLFLDEGFGSLDAESLDIAIQGLELLASGSRMIGVISHVPEVAERLADRIEVVKVGGT
                     STVRDGR"
BASE COUNT          297 a          645 c          764 g          330 t
ORIGIN      
        1 tcgacgactg gcagtcgctg gtggcgggga cggcgatctt cgcgatcgtg aatacgctgc
       61 tgaagccgct cgcgacgatg ctttcgtgct gcctcatcct cgcgacgttc gggctgttcg
      121 tggtggtcat caacgcggcg atgctgggga tcacggcatg ggcggcgggg cagctcgagc
      181 tgaacttcca cgtcgacggg ttctggtcag cggtactggg agccctcgtg atctcagcgg
      241 tcgcgatggt ggcgacgtgg ctgatccggc cgcctcgggt gcagcggttc tagccgcgcg
      301 gaggcgcgct cgagcgcggg gccagcatgt cacgacgaag gcgcggcggt ggattcgcca
      361 ccgtcagcgt cgagtagctg atcgagctga gcgaaggtct cctcgatccc gctctggtcg
      421 cccggggagg cggccgcgct gccggaggca atcgcttcgt cgagcgcttc cttcgagggg
      481 tagaggtcac gcatcacgag cagcgtctgg cccgcgtgct cctcgaaggt gaccgtcgtg
      541 acctgcccgc tgccgccggc ctcgtcattc gaccagacga ggcgtgagtg aggttgcact
      601 tcgaggtagc tgccgtggaa cgccacgggt tccggggccg ccgggtggct gaataggagg
      661 cggtatcctc cgcccacgcg aacgtcgagc tcgcacgaga gcagggtcag cccaaacgac
      721 ttcggcaccc accaccgctg gaacagttcg gccctggtcc aggcttcgaa tacgaagcgc
      781 gcgggggcgt tgaaggtcct cgttacgacc agctcgcggt ccgatgtccg ttccaccgtc
      841 gtgcggccgg ccatggcgtc gggcccgttc cccgttcccg tgtccatcag ttcactccct
      901 gcgtctcagt tcctcgacga ctctgtccaa ttcgtcgaag cgtgcctccc acagttggcg
      961 gtacccctcg atccaggtcg cttcttcctc gaggcggcgt gggccgagcc ggcaggtccg
     1021 cacacgcccg accttttcgg tggtgaccag gcccgcctgc tcgaggacgg cgacgtgctt
     1081 cttgatgccc gtgcgggtca tgcggaactt cacggcaagg tccgtgatgg aagcatccgc
     1141 gcgtccaagc tgctccagca cgccccggcg cgtggcgtcg gagagagcgc cgaaggacgc
     1201 gtcgagccgt gttaaatact gaaccatatg gtgcactata gggccacgcg tggacacggc
     1261 gcaacagccc ccgggtacct cgtgtgatgc gctcaggtgc gggccgaccg gcgaaccttg
     1321 ggccactgcg aggcaggggc gtggaggaag ggttcgctag cggccgtcgc ggacggtgct
     1381 cgtgccgccg actttgacca cctcgatgcg gtcggcgagg cgctcggcga cctcggggac
     1441 gtgcgagatg acgccaatca tgcgcgagcc gctggcgagc agctcgaggc cctggatggc
     1501 gatgtcgagc gactcggcgt cgagggagcc gaagccttcg tcgaggaaga gggagtcgag
     1561 ggagagcgcg ccgccgtggc cggagagctc gggcaggcgc tcggagaggg cgagggcgag
     1621 ggcgaggctg gcgaggaagg tctcgccgcc ggagagggtg cggacggagc ggcgctcgtc
     1681 gccgttgagg cggtcgacga cgacgaactc gtcgccgtcc gcctcgaggc ggtagcggtc
     1741 gcgggagagc tggagcatac gctcgccggc atcggcggcg aggacgtgca tcgcctcgcg
     1801 ctggatgtag gcgacgaagc ggttcgcctg cagctccagg ccgagctcgg aggcgaggtc
     1861 cgaggtgcga cggtaagagc gagcgtcctc gaggagcctc gtcgcctggt ccacctgggc
     1921 gcgtgccgcc tcgagcgtgg ccccggcgcg gccgacttcg gcggcgaggt cggtcgcctt
     1981 gaggccggcg gcctggatca gctcggcgac ggccgtggca tcgggcgcga ggctcg
//
LOCUS       DAORPG010000385         2030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6931_length_2030_cov_3.164557, whole genome shotgun sequence.
ACCESSION   DAORPG010000385 DAORPG010000000
VERSION     DAORPG010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6931_length_2030_cov_3.164557"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLX85_08220"
     CDS             complement(<1..485)
                     /locus_tag="PLX85_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63189.1"
                     /translation="MLRPGGVLRASYQDWKLGVPGFETVMLWSGVDVLLYTYVRRIQV
                     PPVERRYTVVLPKHEQTERLFADALADVADGRRAYGETLLDTGTGIPLLERLSPYAER
                     CSLVEMRRWDTASLCAALERAGFTSVRATVHPGELGRRFAREILREDAMGDFAELFPV
                     AT"
     gene            complement(1133..>2030)
                     /locus_tag="PLX85_08225"
     CDS             complement(1133..>2030)
                     /locus_tag="PLX85_08225"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC63190.1"
                     /translation="RQGVTHQRLGKLIVATSRDDLEQLEAIEARALANEVPGVRRLSG
                     REARSLEPRIPAVEALFSGTTGIVDQMALARSFEAAARHGGATFAYRHRAVAGHRRGG
                     MFHVKLLDDDDALSELSCRQVVNAAGHGAPQVAEALGYPLDGSGQVPVLQQRPNRGRY
                     YDVVNPTVARGVRHLVYPVRREGDAGLGVHITLDVDGGMHLGPDAEWMTAEEPLDYRN
                     DDSRRDEFVSAARRLLPTLRDADLVPGQVGYRPKLSDAPSGEPDFMLWHDEGYVHLGG
                     IESPGLTASIAIARAVATMLRE"
BASE COUNT          351 a          690 c          665 g          324 t
ORIGIN      
        1 gtggcgacgg ggaagagctc ggcgaagtca cccatcgcat cctcgcgcag tatctcgcga
       61 gcaaagcgcc tgccgagttc gcccggatgc acagtcgctc gcaccgaggt aaacccggca
      121 cgctcgagcg cagcgcagag ggatgcggta tcccacctcc gcatctctac gagcgagcag
      181 cgttcggcgt acggcgagag gcgttcgagc aaggggatcc cggtccccgt gtcgaggagc
      241 gtctctccgt acgcgcgcct cccgtcggcc acatcggcga gtgcatcagc gaacagtcgc
      301 tcggtctgtt cgtgcttcgg gagaacgacc gtataccgcc gttcgaccgg cggcacctgg
      361 atgcgacgca cgtacgtgta cagcaggacg tcgacgccac tccacagcat cactgtctcg
      421 aagcccggga cgccgagctt ccagtcctga taagacgccc ggagcacgcc gcccggccgc
      481 agcacacgga cgagttcgcg taacacgctc tctgagtcag cggcctcctc gagcgacgag
      541 gctgcagtca cgaggtcgaa tgaggcgtca tcgaacggca gacgggccgc atccgccgcg
      601 acgaacgtgg cgttcgcgag gcccaggcgt cgtgcgttgg aggtgcatac ctcagtacgg
      661 agaggcgaag cgtcgacacc gacgacggtt acatcgggac gctgcgcggc aaacggcagc
      721 gccggccagc cgtctccggg gccgacatcg aggactctcc tgccctcttc ggggaggtgg
      781 gcgacgtagt cagcgacgcg tgcggcgtcc gcccagtgcg cctcacttct tgggtcgtaa
      841 ggaacgtcaa cgaacggcag ctggccattc cgctgcaggg gcatgagttc gtacaggccg
      901 cgcgctgatt cgacggtttc gggtcgcaga tgcgagtgca accactgttc gacgctgctg
      961 agccggatcg aggccgttgt ctgctcggtc acactctaga agaggctgcc tgttcggatg
     1021 aatcgctcga ccccatacag caccagcagg ccgatggcga cggcgaacca cttccaatag
     1081 aagcgtcccg gatcctcgag gaggttcatg cctcaagcct actcgacgag cttcactcgc
     1141 gcagcatcgt ggccaccgcg cgcgcgatgg cgatcgacgc tgtcaggccc ggcgactcga
     1201 tccctccgag atgcacgtac ccctcgtcgt gccagagcat gaagtcgggc tcgccactcg
     1261 gcgcgtcgct cagctttggc cgatagccca cctgcccggg aacgaggtca gcgtcccgca
     1321 gggtcggcag cagtcgccgg gccgcgctga cgaactcgtc gcgtcgagag tcatcgttgc
     1381 gatagtcgag tggctcctcg gcggtcatcc actccgcgtc cggcccaaga tgcattcctc
     1441 catcgacatc cagggtgatg tggacgccga ggccggcgtc accttcgcgc ctcaccgggt
     1501 agacgaggtg ccgcacgcct cgggcaactg ttggattcac cacgtcgtag tagcggccgc
     1561 gattcggccg ctgttgcagc acggggacct ggccggagcc gtccagcgga tacccgagcg
     1621 cctccgcgac ctgcggagcg ccatgtccgg ccgcgttcac gacctgccgg cagctcagct
     1681 cgctgagcgc atcatcgtca tcgaggagtt tcacgtgaaa cattccgcct cggcggtggc
     1741 cagccacggc gcgatggcgg tacgcgaacg tggccccgcc gtgtcgggcc gccgcttcga
     1801 acgagcgcgc gagcgccatc tggtcgacga tgccggtggt acccgaaaac agggcctcga
     1861 cggcgggaat ccgcggctcg agggaacgag cttcacggcc actcaaccgg cgcaccccgg
     1921 ggacttcgtt cgcgagcgcc cgcgcctcga tcgcctccag ctgctctagg tcatcacgtg
     1981 aagtggcgac gatcagtttg ccgaggcgct gatgcgttac gccttgccgg
//
LOCUS       DAORPG010000386         2024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_6974_length_2024_cov_3.477400, whole genome shotgun sequence.
ACCESSION   DAORPG010000386 DAORPG010000000
VERSION     DAORPG010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6974_length_2024_cov_3.477400"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PLX85_08230"
     CDS             <1..403
                     /locus_tag="PLX85_08230"
                     /inference="COORDINATES: protein motif:HMM:NF039902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRC63191.1"
                     /translation="AITPLVRERVKLPPEVTALVDFLFASDVAAPPRDQLLGKAYRNR
                     ADAAPAAIDAAVAALDALEAWDAAAIEECLRAVAAQTGEKPGDLFMLCRLAVTGKNVT
                     PPLFESMELVGRDASLDRLGRAAEVLRAAAG"
     gene            440..513
                     /locus_tag="PLX85_08235"
     tRNA            440..513
                     /locus_tag="PLX85_08235"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:472..474,aa:Gln,seq:ctg)
     gene            633..1124
                     /locus_tag="PLX85_08240"
     CDS             633..1124
                     /locus_tag="PLX85_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63192.1"
                     /translation="MSMDSYDFTVGARVLTADGQDVGAVKEILGYYFKVDAPMAPDYW
                     LPASLIRSSDQSVITLGADREALGGYEVEPSVIEDERVSEAAPPDEQTMAIEAAVIVW
                     GADEWPVVRRRILEHDARYNEAELDRMQAEEPEETHRRFGHLFEDANRSAERLARTIA
                     ENT"
     gene            1173..1550
                     /locus_tag="PLX85_08245"
     CDS             1173..1550
                     /locus_tag="PLX85_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63193.1"
                     /translation="MVAITILEAAQRRTLIAGVVPDVKTDFAQVMADMRDQGLDPFQG
                     TAALLVGPYPSRRSQPTGHGRPLERAVLIVSGSDPHIEFELELVVGLVPEHVWHEEHV
                     AHWIPTRYSGVPITYGYDLRYIS"
     gene            1581..>2024
                     /locus_tag="PLX85_08250"
     CDS             1581..>2024
                     /locus_tag="PLX85_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63194.1"
                     /translation="MPPDVAEDALLVHAADQAFVDALDEALVALHPNESHWWNHASFA
                     LPLLGAIAFVAIGLLTGKAEATGFGLFLAGITVFMLPVVLLTWRATATAIVLTDRRAL
                     ALHDGRILREVAWADVERIERIETLGNIRWKFLPREGQHLTVEGEI"
BASE COUNT          311 a          755 c          634 g          324 t
ORIGIN      
        1 cgcgatcacg ccgctcgtcc gcgagcgcgt caaactgccc ccggaggtca cggcgctcgt
       61 cgatttcctc ttcgcttcgg acgtggccgc gccgccgcgc gaccaactgc tcggcaaggc
      121 gtaccggaac cgcgccgacg ccgcgccggc cgccatcgat gctgccgtcg ccgccctcga
      181 tgcgctcgag gcgtgggacg ccgcggccat cgaggagtgc ctgcgggcgg tggcggcgca
      241 gaccggcgag aagccgggcg acctcttcat gctttgccgg ctcgccgtga ccggaaagaa
      301 cgtgaccccg ccgctcttcg aatcgatgga gctggtcggc cgcgatgcct cgctcgaccg
      361 cctcggacgc gccgccgagg tgcttcgcgc cgcggccgga tgaccgcatt gcggaagctt
      421 gctagtctgg taactccggt ggggactcgt ctaagggtag gacagctgac tctggatcag
      481 taagtcgagg ttcgaatcct cggtccccag ccacttcccc ggttcgcgca ctgcgagcat
      541 cctgcaatcg tccaaccgct ccgccccacg cacagcgtct cgcatatctc gtacgctctc
      601 gccagccgct ggcacacccc gaggagggcg cgatgagcat ggacagctac gacttcaccg
      661 tgggggcgcg ggttctgacc gctgacggcc aggacgtcgg cgccgtgaag gagatcctcg
      721 gctactactt caaggtggac gcgccgatgg ctccggacta ttggctcccc gcgtcgctca
      781 tccgctcgtc cgaccaatcg gtcatcacgc tgggagccga ccgcgaggcc cttggcggct
      841 acgaggttga accgtcggtg atcgaggacg agcgtgtctc ggaagccgcc ccgcccgacg
      901 agcagaccat ggccatcgag gcagcggtca tcgtctgggg cgcggacgag tggcccgtcg
      961 tccgccggcg catcctcgag cacgacgccc gctacaacga ggccgagctc gaccgcatgc
     1021 aggccgaaga gcccgaggaa acgcaccgac ggttcggcca cctgtttgag gatgccaacc
     1081 gctccgccga gcgccttgcc cgcaccatcg cggagaacac ctgacgagcc acgaccgcct
     1141 gcccgcggcg taccatgacc gcggagccgc gtatggtcgc catcaccatc ctcgaggccg
     1201 cgcagcgtcg caccctcatc gcgggcgtgg tgcccgacgt gaagacagac ttcgcgcagg
     1261 tcatggcgga catgcgcgac cagggcctcg acccgtttca gggcacggcc gccctcctcg
     1321 tggggcccta cccttcgagg cgctcacaac cgaccggtca cggccgcccg ctcgaacggg
     1381 cggtcctcat cgtttcgggc agcgatcccc acatcgagtt tgagttggaa ctggtcgtcg
     1441 gcctcgtccc cgagcacgtg tggcacgagg agcacgttgc ccactggatc ccgacgcgct
     1501 actcgggcgt tcccatcacg tacggctacg accttcgcta catctcctga tccgcccgct
     1561 gccggtggca caatggctgc atgccgcccg acgtagccga ggacgccctg ctcgtccatg
     1621 ccgccgacca ggccttcgtc gacgcgctcg atgaggcgct ggtagccctc caccccaacg
     1681 agtcgcactg gtggaatcac gcctcctttg cgctccccct cctcggtgcg attgcgttcg
     1741 tggccatcgg cctgctgacc ggcaaggccg aggcgaccgg cttcggcctc ttcctggccg
     1801 gaatcaccgt cttcatgctc cctgtcgtcc tcctcacctg gcgcgccacc gccacggcca
     1861 tcgtcctcac cgaccgccga gcgctggccc tccacgacgg ccgtatcctc cgcgaggtcg
     1921 cctgggccga cgtcgagcgc atcgagcgta tcgagaccct gggcaatatt cgctggaagt
     1981 tcctcccccg cgagggccaa cacctcacgg tcgagggcga aatc
//
LOCUS       DAORPG010000387         2012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7049_length_2012_cov_3.375575, whole genome shotgun sequence.
ACCESSION   DAORPG010000387 DAORPG010000000
VERSION     DAORPG010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7049_length_2012_cov_3.375575"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..1052
                     /locus_tag="PLX85_08255"
     CDS             42..1052
                     /locus_tag="PLX85_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63195.1"
                     /translation="MSWVIAFTDPGSVYPVIDPRRFGGDRPLMFGYGFAQDLVRVDLR
                     ENPNITNCQMLVDLEVRPSVALPKNIQAWNYASGTVSSIHAGGAMLLNRADGPVGTHT
                     LLLNGLYSFRSSELWDLWGGYTVTFRWIRDVGFNGPWGSQMPAPTYSTADDGLLIRGS
                     RHPERVFVMFGRAKFRIDARNALGFDVNSARIVDQETVDSLTSMPADYTVLRDRAYPT
                     YFVCFGGARFPFRPGDPLLNSNFPVGLDLHLGFTDVRIQAAAKTVPDGASNSIPRVPR
                     DGTLLRDAGDPASNVYLVQGGLLRRVASPAAFAANCLATRNIRTVPAGSLTSLPHGAD
                     LT"
     gene            1073..1474
                     /locus_tag="PLX85_08260"
     CDS             1073..1474
                     /locus_tag="PLX85_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006538308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRC63196.1"
                     /translation="MLPTPEDHVAITDLLARYCLTLDRDDVEAWVALFTPDAAYEVYG
                     RAFAGHDGLRRMLAAAPGGLHLGGPPVIDMLDADHARTTRNLLFIERGGGTQRSAVYS
                     DDLVRTAEGWRIARVRCQFITAGGLSDRPAE"
     gene            1555..>2012
                     /locus_tag="PLX85_08265"
     CDS             1555..>2012
                     /locus_tag="PLX85_08265"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRC63197.1"
                     /translation="MDRYAFHIVNNLAGHNRLIDDVMIDWSKGGPLVLGMIVVAAWFL
                     LPARSASVQPRRLAFISVVAALAALGLAQLVSHEWFRERPYVVAAPVHLLVAPSPDGS
                     FPSDHAVGGFALATPLLLDRRARRFGLVVLGGALLLALSRVYVGTHYPGDV"
BASE COUNT          285 a          717 c          672 g          338 t
ORIGIN      
        1 gttgcacgcc cgacgggtga ggcgaaggcc gaggacgcgg aatgagctgg gtgattgcct
       61 tcacggaccc cggcagcgtg tatccggtca tcgaccctcg tcgcttcgga ggcgaccgtc
      121 cgttgatgtt cgggtacggg ttcgcgcagg acctcgtccg cgtggacctc agggagaacc
      181 cgaacatcac gaactgccag atgctcgtcg acctcgaggt ccgtccgagt gtcgcccttc
      241 ccaagaacat ccaggcctgg aactacgcca gcggcaccgt cagttcgatt cacgccgggg
      301 gcgcgatgct gctgaatcga gcggacggcc cagtcgggac gcataccctc ctgctgaacg
      361 gcctctactc gttccggtcg agcgaattgt gggacctctg gggcggctac acggtcacct
      421 tccgctggat cagggatgtc ggattcaacg gcccgtgggg ctcgcagatg cccgcgccaa
      481 cctactcgac agcggatgat ggcttgctga tccggggtag caggcacccc gagcgcgtct
      541 tcgtcatgtt cggcagggcg aagttccgca tcgacgcgcg gaacgcgctc ggcttcgacg
      601 tgaacagcgc tcgtatcgtc gaccaggaga cggtcgatag cctcacgtcg atgcccgcgg
      661 actacaccgt gctgcgcgac cgtgcctatc ccacgtactt tgtctgcttt ggcggcgctc
      721 gctttccgtt caggcccggc gatccgctgc tcaacagcaa cttcccggtg ggcctcgacc
      781 tgcacctcgg gtttacggac gtgcgcatcc aggccgccgc caagaccgtc ccggacggcg
      841 cctcgaactc gatccctcgc gtcccacgcg acggcacgtt gctccgcgat gccggcgacc
      901 cggcatcgaa cgtgtacctc gtgcaggggg gcctgctgcg ccgcgtcgcc tcgccggccg
      961 cgttcgcggc gaactgcctc gccacgagga acattcgcac ggtaccggcc ggctcgctca
     1021 cctcgctccc gcacggcgcg gatctcacgt aaccggacgg atcgaggtgg ccatgctccc
     1081 cacgcccgag gaccacgtcg cgatcaccga cctgctcgcc cgctactgcc tcacgctcga
     1141 ccgggacgac gtcgaggcgt gggtcgcgct gttcacccca gacgctgcgt acgaggttta
     1201 cgggcgggcg ttcgcgggcc atgacgggct gcgacggatg ctcgcggcgg ccccgggtgg
     1261 gctccacctc ggcgggcccc cggtgatcga catgctcgac gccgaccacg cgcggacgac
     1321 ccgcaacctg ctgttcatcg agcgcggggg cggtacgcag cgcagcgccg tgtacagcga
     1381 tgacctcgtg cgtaccgccg agggctggcg catcgcgcgc gtccgatgcc agttcatcac
     1441 cgccgggggg ctgagcgacc gtcccgctga atagccgggc gctcctccgc gcgggcgtcg
     1501 acctcccccg tcgtcgtacg atcgatgcga acgtgagccc gggaacgagg gagcatggac
     1561 cgctacgcct ttcacatcgt gaacaacctg gcggggcata accggctgat cgacgacgtg
     1621 atgatcgact ggtccaaggg cgggccgctc gtcctcggca tgatcgtcgt ggccgcctgg
     1681 ttcctgctgc cagcgcgatc ggcgtcggtg cagccacgcc ggctcgcctt catctcggtc
     1741 gtcgcggcgc tggcggcgct cggactggcg cagctcgtca gccacgaatg gttccgggag
     1801 cgcccctacg tggtcgccgc gccggtccat ctcctggtcg ccccgtcacc ggacggctcg
     1861 ttcccctcag accacgcggt tggcgggttc gcgctcgcga cgccgctgct gctggaccgg
     1921 cgcgcacggc gcttcggcct cgtcgtgctc ggcggcgcgc tgctgctcgc gctgtcgagg
     1981 gtgtacgtcg ggacgcatta cccgggcgac gt
//
LOCUS       DAORPG010000388         2007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7080_length_2007_cov_3.072234, whole genome shotgun sequence.
ACCESSION   DAORPG010000388 DAORPG010000000
VERSION     DAORPG010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7080_length_2007_cov_3.072234"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PLX85_08270"
     CDS             complement(<1..413)
                     /locus_tag="PLX85_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63198.1"
                     /translation="MRRSVESTQHYYEIGQRSVYGLLTFARRAVDVVSWLSVLLLAGA
                     VFAYVGLAPDLSLASRVVVAVLLFTPPLALLHFAMVLRLLRLRFDPFGSGIWLRLLTL
                     SRLVGAGLLLSPLYWSSVVVLGFSSLIVVPFAVVIS"
     gene            complement(436..975)
                     /locus_tag="PLX85_08275"
     CDS             complement(436..975)
                     /locus_tag="PLX85_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63199.1"
                     /translation="MAVIGIIALVLLLIVAALAVLWMQRRRRSNALREQFGPEYDRAV
                     AEAGGRRQAEADLDQRRRRVEELQIVELSPEESDAFTRQWNQVQLRFVDRPAEAIGEA
                     DQLVARVMERRGYPVSDFDRRAADISVDHPEVVSEYRDARAVAVKNADGGASTEELRQ
                     ALLHYRALVGELLEKTAGA"
     gene            1126..>2007
                     /locus_tag="PLX85_08280"
     CDS             1126..>2007
                     /locus_tag="PLX85_08280"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRC63200.1"
                     /translation="MTTIAPSEAITKAAAAKAAGRRLAYASTDQKNEALHRIADALLA
                     DQAHLLEVNAGDVQRGQERGLSDSFVDRLALTPERIAGIAADTRQVATLEDPIGHIEE
                     MRTRPNGLQIGRMRVPLGVIGAVYESRPNVTVDIAALCLKSGNATVLRSGSDAHATSS
                     ALAAIVMRSIEAAGLPAGIVQFIDSTDRDEVGAMLKAHEYIDLMIPRGGADLIRRVRD
                     EATMPVVAGGIGVCHTYIDASADLEMARRIVVNAKTRRPSICNALDTVLVHAAIADQL
                     LPELSRELGERGVLLHTD"
BASE COUNT          346 a          780 c          624 g          257 t
ORIGIN      
        1 gagatcacga cggcaaacgg caccacgatc aggctcgaaa acccgagcac cacgaccgaa
       61 gaccagtaca gcgggctcag cagcaacccg gcgccaacga ggcggcttaa cgtcagcaga
      121 cgtagccaga tgcccgaacc gaacgggtcg aagcgcagcc ggagcagccg cagcaccatc
      181 gcgaagtgca acagcgccag cggcggcgtg aacagcagca ccgccaccac gactcgcgag
      241 gcgagcgaca ggtcgggcgc gaggcccacg tacgcgaaga ccgcgcccgc gagcagcagc
      301 accgacagcc agctcaccac gtccaccgcc cgcctcgcga acgtcagcag gccatacacg
      361 ctccgttgac cgatctcgta gtaatgctgc gtcgactcga cactgcggcg cacgggcaga
      421 tcccttcgct ggccctcagg cccccgccgt cttctcaagc agctcgccga cgagcgcgcg
      481 gtagtgcagc agggcctgcc gcaactcctc ggtgctggcg ccgccgtcgg cgttcttcac
      541 tgccaccgcg cgggcgtcgc ggtactccga caccacctcg gggtggtcga ccgagatgtc
      601 cgcggcgcgt cggtcgaagt cggacaccgg gtagccgcgc cgctccatca cgcgcgcaac
      661 cagctggtcg gcctcgccga tcgcctccgc cggccggtcg acgaagcgca gctgcacctg
      721 gttccactgg cgcgtgaacg cgtcggactc ctcgggcgac agctcgacga tctgcagctc
      781 ctcgacgcgc cggcgacgct ggtcgaggtc cgcctcggcc tgccggcgtc caccggcttc
      841 cgccaccgcc cggtcgtact cgggcccgaa ctgctcccgc agcgcgttcg aacgccgacg
      901 ccgctgcatc cacagcaccg cgagcgccgc gacgatgagc aacagcacca gcgcgatgat
      961 cccgatgacc gccatgggac gcctcctcgc attccgtctc ctcatctaag ccgcgcgccg
     1021 ttcgcatcca cggaaacgaa agcgcgctcc tattgcggac tcctcgcgag gagcagcgtc
     1081 ccgccggctc tccacctccg ctgcttgccc tacaatgtcc cgactatgac gacgatcgcc
     1141 ccttccgaag ccatcaccaa ggccgccgcg gcgaaggccg cggggcgccg cctcgcctac
     1201 gcgagcaccg accagaagaa cgaggcgctg catcgcatcg ccgacgcgct gctcgccgac
     1261 caggcgcatc tcctcgaggt caacgccggg gacgtacagc gcggccagga gcgcgggttg
     1321 agcgactcct tcgtcgaccg cctcgcgctc acccccgagc gcatcgcggg catcgccgcc
     1381 gacacgcggc aggtcgcgac cctcgaagac cccatcgggc acatcgagga gatgcgcaca
     1441 cgtccgaacg gcctgcagat cgggcgcatg cgggtgccgc tcggcgtcat cggcgccgtc
     1501 tacgagtcgc ggcccaacgt gaccgtcgat atcgccgcgc tctgcctcaa gtcgggcaac
     1561 gccactgtgc tgcgttcggg ctcggacgcc cacgccacct cgtccgcgct tgccgcgatc
     1621 gtcatgcgca gcatcgaggc ggccggactg ccggccggca tcgtccagtt catcgactcc
     1681 accgaccgag acgaggtcgg cgcgatgctc aaggcgcacg agtacatcga cctgatgatt
     1741 ccgcgcggcg gcgccgacct catccggcgc gtccgcgacg aggcgaccat gccggtcgtg
     1801 gccggcggta tcggcgtctg ccacacctac atcgacgcga gcgcagacct cgagatggcg
     1861 cgccgcatcg tggtcaacgc gaagacccga cggccctcga tctgcaacgc cctcgacacc
     1921 gtgctcgtgc acgctgcgat cgcggaccag ctgctgcccg agctctcacg cgagctcggc
     1981 gagcgcggcg tcctgctgca caccgac
//
LOCUS       DAORPG010000389         2005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7090_length_2005_cov_3.793846, whole genome shotgun sequence.
ACCESSION   DAORPG010000389 DAORPG010000000
VERSION     DAORPG010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7090_length_2005_cov_3.793846"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="PLX85_08285"
     CDS             complement(<1..1049)
                     /locus_tag="PLX85_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRC63201.1"
                     /translation="MEQVGLLPAILAGAFVVVALFNAFLPRRGDWLVILAMLAVIVIG
                     FLVIKDFQTYFHDGEFMPRGENAYSFEWVNIGDGLFVIDFSTWVDSITVVMITVVSIV
                     ALMVMVYSVGYMHGEQRYGWYYAVLALFTAAMLTLVISGNLLITYFAWEGVGLASFLL
                     IGFYWERQSAAEAAKKAFITTRIGDVGFLIGIILLWREAGTFDIQQIIHFAEEGGFGE
                     TYLTVTMLFLFMGAVGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVVAGVYLVARMM
                     PVFDQVLIARDVVLYTGLITAAMAATMGLVASDIKRVLAFSTVSQLGFMFVALGLGSV
                     SAAMFHLFTHAFFKAL"
     gene            complement(1100..1408)
                     /gene="nuoK"
                     /locus_tag="PLX85_08290"
     CDS             complement(1100..1408)
                     /gene="nuoK"
                     /locus_tag="PLX85_08290"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRC63202.1"
                     /translation="MIPLENFLVLSGILFAIGFYGVLARRSAILILVSVELMLAAVSI
                     NFVAFASYLDPDFFTGIIFSIFVIVVAAAEVGLALGIVLRAYRERATANVDEANNLKW
                     "
     gene            complement(1405..>2005)
                     /locus_tag="PLX85_08295"
     CDS             complement(1405..>2005)
                     /locus_tag="PLX85_08295"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRC63203.1"
                     /translation="QIDRVIVGGSLVAGAVLVPVLVVLIALLGADRISGPELVWWLLA
                     LIIVAAALGAVLLRNVVRAALCLVIVALGIAGMFLLLASEFLALVQVLVYGGGVTILL
                     LFGLMLTNAQDDPVVQDGSQKPFAFGVGIALGAVIVIAMTDATWNTGTRSVVAFRALG
                     DRLYRDFGVPFEVASLVLLVALVGAIAISRRDRDEEEVA"
BASE COUNT          431 a          640 c          673 g          261 t
ORIGIN      
        1 agcgccttga agaaggcgtg ggtgaacagg tggaacatcg cggcggagac cgagccgagg
       61 ccgagcgcca cgaacatgaa gccgagctgc gacaccgtcg agaaggcgag cacgcgcttg
      121 atgtcgctgg ccacgaggcc catggtcgcg gccattgccg ccgtgatcag gcccgtgtac
      181 agcacgacgt cgcgcgcgat cagcacctgg tcgaagaccg gcatcatgcg cgccaccagg
      241 tagacgcccg cgacgaccat cgtggccgcg tggatgaggg cgctgacggg cgtcgggccc
      301 tccatcgcgt ccggcagcca gacgtgcagc gggaactgcg ccgacttgcc gacggcgccc
      361 atgaacagga agagcatcgt cacggtgagg taggtctcgc cgaagccgcc ctcctccgcg
      421 aagtggatga tctgctggat gtcgaacgtc ccggcttccc gccagagcag gatgatgccg
      481 atcaggaagc cgacgtcgcc gatgcgggtg gtgatgaagg ctttcttggc cgcttccgcc
      541 gcggactgcc gctcccagta gaaaccgatg agcaggaacg aggcgaggcc gacgccttcc
      601 caggcgaagt aggtgatgag caggttgccg gagatgacca gcgtcagcat cgcggccgtg
      661 aacagcgcca gtaccgcgta gtaccaaccg tagcgctgct cgccgtgcat gtagccgacc
      721 gagtagacca tcaccatcag ggccacgatg gagacgacgg tgatcatgac cacggtgatg
      781 ctgtcgaccc acgtactgaa gtcaatgacg aacaggccgt cgccaatatt gacccactcg
      841 aaggagtagg cgttctcgcc tcgaggcatg aactcgccgt cgtggaagta ggtctggaag
      901 tccttgatga cgaggaagcc aatgacgatc acggcgagca tcgcgaggat gacgagccag
      961 tcgccgcgac ggggcaggaa cgcattgaag agcgccacga ccacaaatgc ccccgcgagg
     1021 atcgcgggaa gcaggcctac ctgctccatg cccatcgaga tgccccctac gcctggttac
     1081 gcgacgacgg acgccgcgac taccacttga ggttgttcgc ctcgtccacg ttcgcggtgg
     1141 cgcgctcgcg gtaggctcgc aacacgatgc cgagcgccag gccgacttcg gccgcggcga
     1201 cgacgatcac gaagatcgag aagatgatgc ccgtgaagaa gtccgggtcg aggtaggagg
     1261 cgaaggcaac gaagttgatc gagactgccg cgagcatcag ctccacggag accaggatga
     1321 ggatggcgct ccgtcgcgcc agcaccccgt agaacccgat cgcgaacagg atccccgaga
     1381 ggaccaggaa gttctccagc gggatcacgc gacttcctcc tcgtcccggt cacgcctcga
     1441 aatcgcaatt gcgccgacca gcgccaccag cagcaccaac gaggcgacct cgaaggggac
     1501 gccgaagtcg cggtagagcc ggtcgcccag cgcgcggaac gcgacgaccg aacgggtgcc
     1561 ggtgttccag gtagcgtccg tcatcgcgat gacgatgacc gcgccgagcg cgatgccgac
     1621 gccgaacgcg aacggcttct gggagccatc ctggacgacc gggtcgtcct gcgcgttcgt
     1681 gagcatgaga ccgaagagga gaaggatggt gacgccgccg ccgtacacga ggacctgtac
     1741 gagggcaagg aactcggacg cgagcagcag gaacataccc gcgataccga gcgcgacgat
     1801 gacgagacac agggcggcgc gcacgacgtt ccgcagcagc actgcgccga gcgcggcggc
     1861 gacgatgatc agcgcgagca gccaccagac gagttcgggc ccgctgatgc ggtccgcgcc
     1921 cagcagtgca atgagcacga ccagcaccgg caccaggacg gcgccggcca caagactccc
     1981 gccgacaatg acgcggtcga tctgc
//
LOCUS       DAORPG010000390         2002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7107_length_2002_cov_3.392912, whole genome shotgun sequence.
ACCESSION   DAORPG010000390 DAORPG010000000
VERSION     DAORPG010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7107_length_2002_cov_3.392912"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            318..1472
                     /gene="hflX"
                     /locus_tag="PLX85_08300"
     CDS             318..1472
                     /gene="hflX"
                     /locus_tag="PLX85_08300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRC63204.1"
                     /translation="MSTAPVKERAYLVGLDHGQNGLMTEEESLRELGRLARTAGTVVV
                     GQSVQRRPHPDPATYVGSGKLEEIKSARERTNYSLVVFDEQLSPAQQLRLESALEVKV
                     LDRSALILDIFASRARTREARLQVELAQHEYLLPRLRGQWQHLERMEGAVGLRGPGET
                     QLETDRRLIGQRIARLKREIDAVRRQRALHRRRRTREGVPVVALVGYTNAGKSSLLRT
                     LAGAEVLAADTLFATLDPLTRRVGLPGNETILVTDTVGFIQKLPTQLISAFRATLEEL
                     ESADLLLHVVDATSPAIEAQWATVMETLDGLGLAEKPMLTVLNKADGLTRNDGEPVTS
                     EADLARVEVTSPAPGAALLVSAVEGWGIDALRRRLAFELFGVRPEVSTLA"
     gene            complement(1469..1777)
                     /locus_tag="PLX85_08305"
     CDS             complement(1469..1777)
                     /locus_tag="PLX85_08305"
                     /inference="COORDINATES: protein motif:HMM:NF037043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63205.1"
                     /translation="MESPFARRLRVWEWLPFSARRLEERLRALGFGRVEVGRRGSPVD
                     TNVLERQLNRRIGRATTRERNPLRHGPPGPGNSGRVATVLLTRVRDEHVALICERERD
                     "
     gene            1806..1934
                     /gene="rpsQ"
                     /locus_tag="PLX85_08310"
                     /pseudo
     CDS             1806..1934
                     /gene="rpsQ"
                     /locus_tag="PLX85_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458351.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
     gene            1939..>2002
                     /locus_tag="PLX85_08315"
     CDS             1939..>2002
                     /locus_tag="PLX85_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL14 family ribosomal protein"
                     /protein_id="HRC63206.1"
                     /translation="MIQMQTNLDVADNSGAKRVMC"
BASE COUNT          330 a          800 c          592 g          280 t
ORIGIN      
        1 ccggccgcct catcgacgcc accggtgtcg tcgtcactcc cggcgtgagc tacggcccgg
       61 caggggaggg ctacatccgc atctcgctca cgctcgccga cgaccgcgtc gaggagggcc
      121 tgcgccgcct ctccgccttc gcacggggag agcaggtgct cgacacctga gcgaatgcgc
      181 tacgatcgag gtcagcgtgc atcccacgga ggtcactgat ccctcgacgt cgcccgttcc
      241 agacgaacac tcctaccccc gccacggacg gcgcccgctc gaatggctcg aggagcaacg
      301 gcgctcgagg gaacggcgtg tcgaccgctc cggtcaagga gcgcgcctac ctcgtcggcc
      361 ttgaccacgg ccagaacggc ctcatgacgg aggaggagtc cctccgcgag ctcgggcgcc
      421 tcgcgaggac cgcgggcacc gtcgtcgtcg gccagagcgt ccagcgccgt ccccaccccg
      481 accccgcaac ctacgtcggt tcgggcaagc tcgaggagat caagagcgcc cgcgagcgca
      541 ccaactacag cctcgtcgtc ttcgacgagc agctctcgcc tgcccagcag ctccgactcg
      601 agtccgccct cgaggtgaaa gtcctggatc gcagcgccct catcctcgac atcttcgcca
      661 gccgcgcccg cacccgcgag gcgcgcctgc aggtcgagct cgcccagcac gagtacttgc
      721 tcccacgcct ccgagggcag tggcagcacc tcgagcgcat ggaaggcgcc gtcggcctcc
      781 gaggccccgg tgagactcag ctcgagaccg accgccggct catcggccag cgcatcgccc
      841 gcctcaagcg ggagatcgat gccgtgcgcc gccagcgcgc cctgcaccgc cgccgacgca
      901 cccgcgaggg cgttccggtc gtcgccctcg ttggctacac gaacgccggc aagtcctcgc
      961 tcctgcgtac gctcgctggc gccgaggtgc tcgctgcgga cacgctcttc gctacgctcg
     1021 accccctgac ccgccgcgtc gggctgccgg gcaacgagac catcctggtc acggacaccg
     1081 tgggcttcat ccagaagctc cccacccagc tcatctccgc cttccgcgcg accctcgagg
     1141 agttggagag cgccgacctc ctcctgcacg tcgtcgacgc cacctccccg gccatcgagg
     1201 cgcagtgggc gacggtgatg gagaccctcg atggcctcgg cctcgcggag aagccgatgc
     1261 tcaccgtcct caacaaggcc gacggcctga ctcgcaacga cggtgagcca gtcacctccg
     1321 aggccgatct cgcccgggtc gaagtcacga gcccagcccc cggcgccgcc ctcctcgtct
     1381 ccgctgtcga aggctggggc atcgacgccc tccgccgccg cctcgccttc gagctcttcg
     1441 gcgtccgccc cgaggtctca acgctcgcct agtcccgctc ccgctcgcag atcagcgcca
     1501 catgttcgtc ccgcacgcga gtcaagagca cagtcgccac tcggcccgaa ttccccggcc
     1561 cggggggacc gtgtcgtagc gggttcctct ccctcgtcgt ggcccgccca atccgccggt
     1621 tcaactgccg ctcgaggacg ttggtatcca cgggcgaccc ccgtcgtccc acctccaccc
     1681 gcccgaaccc gagcgcccgc agccgctcct ccagccgccg cgccgaaaac ggaagccact
     1741 cccacacccg caaccgccgc gcgaacggcg actccaccgc agcctctgaa gtcaggtacg
     1801 cgatccgctc gtcgagcttc cacgcccatg acgaagccaa cgccttcaag gaaggcgaag
     1861 tggtcaacat tcaagaatgc ccgccgaagt ccaagctgaa acgctgggaa gtcgtcggcc
     1921 gcgttggagc atgacgccat gatccaaatg caaaccaatc tcgacgtcgc cgacaattcc
     1981 ggcgccaagc gcgtgatgtg ca
//
LOCUS       DAORPG010000391         1998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7134_length_1998_cov_3.295419, whole genome shotgun sequence.
ACCESSION   DAORPG010000391 DAORPG010000000
VERSION     DAORPG010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7134_length_1998_cov_3.295419"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLX85_08320"
     CDS             complement(<1..185)
                     /locus_tag="PLX85_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRC63207.1"
                     /translation="MTTTDAGQLPEVAIRRGLKDVYFDRTRASYIDGRAGKLLYRGYS
                     IHDLADHSTFEETAYLL"
     gene            complement(295..1989)
                     /gene="acsA"
                     /locus_tag="PLX85_08325"
     CDS             complement(295..1989)
                     /gene="acsA"
                     /locus_tag="PLX85_08325"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011190396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRC63208.1"
                     /translation="MAPNLMDLDRLRSEWSWDAVRAELDLPNGLVNQAHECIDRHAAA
                     TPDKVAMIWEGSDGAIERYTFEEMRRQTNRFANVLKRIGIQKADRVFVFADRIPELYF
                     AVFGTLKAGAISAPLFSAFGPEPVAERVRRAEGAMIVTTPHLLPKIDEIRGELPSVRH
                     VMVIGHRAGATVGAANLDYAALMAEASDDFAMEPTGAEDWSIMHFTSGTTGLPKGAAH
                     VHEAVVNHYATGKYALDLHPDDVYWCTADPGWVTGTSYGMFAPWSNGVTQVIYEGGFG
                     AGRWFDIIERHRVTVWYTAPTAIRMLMKAGSGVPRERDLSSLRFTASVGEPLNPEGVV
                     WGEEATGLPFHDNWWQTETGAIMIANYASMDIKPGSMGRPFPGITAGILDDEFNEVTE
                     PMREGQLAIKTPWPSMFRTYWSDEERYRSRFKGEWYITGDRAKRDEDGYFWFVGRDDD
                     VINTSGHLVGPFEVESALIEHPAVAEAGVIGKPDPIAMEIVKAFVSLKDGFEPSDQLR
                     RELIGHTRQRLGPGVAPREIDFIPSLPKTRSGKIMRRLLKARELGLPEGDTSTLED"
BASE COUNT          319 a          607 c          736 g          336 t
ORIGIN      
        1 aagaggaggt aggccgtctc ctcgaacgtg gaatggtcgg cgaggtcatg gatggagtag
       61 ccgcggtaga ggagcttgcc ggcgcgaccg tcgatgtagg aggcgcgggt acggtcgaag
      121 taaacgtcct tgagaccgcg gcggatggcg acctcgggga gttggccggc gtcggtcgtc
      181 gtcactcgtt cctccgaacg atggggccgg gcaccgagcc cggcgcacat gacgtacttc
      241 ggacaccccc atcgtacgcc ggtgacctcg cacggacgtc gcgcgtgcgc gcggtcagtc
      301 ctccaacgtc gaggtgtcgc cctcaggcag gccgagctca cgcgccttca gcaggcggcg
      361 catgatcttg cctgagcgtg tctttggcag agagggaatg aagtcgatct cgcggggcgc
      421 gacgccgggg ccgaggcgct ggcgggtgtg gccgatcagc tcgcgacgga gctggtcgct
      481 gggctcgaag ccatccttca gcgagacgaa cgccttcacg atctccatag cgatggggtc
      541 gggcttgccg atgacgcccg cctcggcgac ggcggggtgc tcgatgagcg cgctctccac
      601 ctcgaaagga ccgacgaggt ggccggacgt gttgatcacg tcgtcatcgc ggccgacgaa
      661 ccagaagtag ccatcctcgt cgcgctttgc gcggtcgccg gtgatgtacc actcgccctt
      721 gaagcgggag cggtatcgct cttcgtcgct ccagtaggtg cggaacatcg agggccaggg
      781 cgtcttgatg gcgagctgcc cctcgcgcat cggctcggtg acctcgttga actcgtcgtc
      841 gaggatgccg gcggtgatgc cggggaaggg gcggcccatg gagccgggct tgatgtccat
      901 ggaggcgtag ttggcgatca tgatcgcgcc ggtctcggtc tgccaccagt tgtcgtggaa
      961 ggggaggccg gtggcctcct caccccacac aacgccctcg gggttcagcg gctcgccgac
     1021 cgaggcggta aagcgcagcg aggagaggtc gcgctccctc ggaacgccgg aacccgcctt
     1081 catcagcatg cggatggcgg tgggggcggt gtaccagacg gtgacgcggt ggcgctcgat
     1141 gatgtcgaac cagcggccgg caccgaagcc gccctcgtag atgacctggg tcacgccgtt
     1201 gctccacggc gcgaacatgc cataggacgt gcccgtcacc cagccagggt cggcggtaca
     1261 ccagtagacg tcgtccggat gcaggtcgag ggcgtacttg ccggtggcgt agtggttcac
     1321 gaccgcctcg tggacgtggg cggcgccctt cggcaggccc gtggtgcccg aagtgaagtg
     1381 catgatcgac cagtcctcgg cgccggtggg ctccatggcg aagtcgtcgg aggcctcggc
     1441 catgagggcg gcgtagtcaa ggttggcggc gcccacggtg gcgcctgcgc ggtggccgat
     1501 gaccatgacg tggcgtaccg agggcaactc acctcggatc tcgtcgatct tcggaaggag
     1561 gtgcggcgtc gtcacgatca tcgcgccctc ggcacgccgg acgcgctcgg cgacgggctc
     1621 ggggccgaag gcagagaaca acggtgctga aatggcgcca gctttgaggg tgccgaagac
     1681 ggcaaagtac agctcgggga tgcggtcggc gaagacgaag acgcggtcag ccttctggat
     1741 gccgatgcgc ttcagcacgt tggcaaaacg gttcgtctgg cgtcgcatct cctcgaaggt
     1801 gtagcgctcg atggcgccgt cggagccttc ccagatcatc gcgaccttgt ccggggtcgc
     1861 ggcggcatgg cggtcgatgc actcgtgggc ctggttcacg aggccgtttg ggaggtcgag
     1921 ttcggcgcgg acggcgtccc agctccattc ggaccgaagt cgatcgaggt ccatcagatt
     1981 gggggccacg gtcgcctc
//
LOCUS       DAORPG010000392         1997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7146_length_1997_cov_3.014418, whole genome shotgun sequence.
ACCESSION   DAORPG010000392 DAORPG010000000
VERSION     DAORPG010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7146_length_1997_cov_3.014418"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PLX85_08330"
     CDS             complement(<1..701)
                     /locus_tag="PLX85_08330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HRC63209.1"
                     /translation="MRGVGLMEFGGPEVLQIVDLPEVHAGPGEVRLRVQAATINPTDT
                     GLRGGLRAEAMRRDPPPYIPGMDAAGVIDEVGPDTDTSLAVGDLVMAMVLPSGSHGAY
                     RESIVLTADSVVRAPKGATAAEASTLPMNGLTARQSLDQLALQPGQTIAVTGAAGCYG
                     GYVVQLAKADGLRVIADASPADEALVRSLGADVVVPRGDDIAARIRAVAPEGVEGLAD
                     GAVQQELAIGAVRDGG"
     gene            871..>1997
                     /locus_tag="PLX85_08335"
     CDS             871..>1997
                     /locus_tag="PLX85_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRC63210.1"
                     /translation="MRRVVLLVVLLGLGVTLIGNARFDSGSSAGASEERTASSTASQA
                     ATVDRATLDSVLAGLARAPSSVIAANDAIGADRWQVAGFTGYGVRVAIVDTGFDGYQS
                     QLGVTLPGTVHARSFRADGRLESGSEHGTLAARIVHSIAPRADLYLANFSTVEEFSNL
                     VAYLQAEQVQVVSFSLGFVHNGPGDGTGPVDDIIGRSVASGQLWTVAAGNWAQQHWSG
                     TFTDRDGNGINDFAPGVEDNGRAYQAGDLITVSLRWDDSWGFACTDYDLELFGPDGAL
                     AAASRGSQACLGNPVESVQVLATKTGRYRVRIVQVGSSGRTPRLDLLMLGAPDRSQPL
                     DYFVTAGSLAEPADHAGVVTVGAENPFDNALVANFSSVGPT"
BASE COUNT          256 a          602 c          831 g          308 t
ORIGIN      
        1 ccgccgtcgc ggacggcccc gatggcgagc tcctgctgca cggcgccatc ggcgaggcct
       61 tcgacaccct cgggagcgac tgcgcggatg cgagcggcga tgtcgtcgcc gcgggggacg
      121 acgacgtcgg cgccgagcga gcggacgagg gcctcgtcgg ccggtgaggc gtcggcgatg
      181 acgcggaggc cgtcggcctt cgcgagctgg acgacgtagc cgccgtaaca gccggcggcg
      241 ccggtgacgg cgatggtctg tccgggctgg agcgcgagct ggtcgaggga ctggcgggcg
      301 gtgagcccgt tcatcgggag ggtggaggcc tcggcggcgg tggccccttt cggcgcgcgg
      361 acgacggaat ctgcggtgag aacgatgctc tcgcggtacg caccgtggct accgctggga
      421 aggaccatgg ccatgacgag gtcgccgacg gcgaggctgg tgtccgtgtc ggggccgact
      481 tcgtcgatga cgccggcggc gtccatgccg gggatgtagg gcggtgggtc ccggcgcatg
      541 gcctcggcgc gcaggccgcc tcgaaggccg gtgtcggtgg gattgatggt cgcggcctgg
      601 acgcggaggc ggacttcgcc cgggccggcg tggacctccg ggaggtcgac gatctggagg
      661 acctcggggc cgccgaactc catcagtcct acgccgcgca tggtggtctc cctttcgtct
      721 cgcgtggtgt ccgcgcgtga ttatgccgcg ccgcgtggtc cggcgcgtgc tcggatgttc
      781 gcgggtttga gcccggccgc gggcatacaa taaatcgcat ctcctcgggg tgcggcttgc
      841 ctcccgtgcc cgttacggac aaagggcagc atgcgcaggg tcgtcctgct ggtggtcttg
      901 ctggggctcg gcgtcaccct catcggcaac gctcggttcg actcggggtc gtccgcgggg
      961 gcgtcggagg aacgcacggc ttcgtcgacg gcgtcgcagg cagcgacggt ggaccgggcg
     1021 acgctggaca gcgtgctggc ggggctcgcg cgggcgccct cgagcgtgat cgcggcgaac
     1081 gatgcgattg gcgctgaccg ctggcaggtg gcggggttca cggggtacgg cgtgcgcgtc
     1141 gcgatcgtgg acacggggtt cgacggctac cagtcacagc tcggtgtgac gttgccgggg
     1201 acggtgcacg cacgatcgtt ccgggcggac gggcggctgg agtcggggag cgagcacggg
     1261 acgctggcgg cgcggatcgt gcacagcatc gcgcctcgcg cggacctcta tctcgcgaac
     1321 ttctcgacgg tggaggagtt ctcgaacctg gtcgcgtacc tgcaggcgga gcaggtgcag
     1381 gtggtgtcgt tctcgctggg gttcgtgcac aacggacccg gggacgggac cgggccggtc
     1441 gacgacatca tcgggcggtc ggtggcctcg gggcagctgt ggaccgtggc ggcggggaac
     1501 tgggcgcagc agcactggtc ggggacgttt acggaccggg acgggaatgg gatcaacgac
     1561 ttcgcgccgg gagtcgagga caacggccgg gcgtaccagg cgggcgacct gatcacggtg
     1621 tcgctgcggt gggacgactc ttgggggttc gcctgcaccg actatgacct ggagctgttc
     1681 gggccggacg gggcgctggc ggcggcgtcg cgggggagcc aggcgtgcct cgggaatccg
     1741 gtggagagcg tgcaggtgct ggcgacgaag accggccggt accgagtgcg gatcgtgcag
     1801 gtggggtcgt cggggcggac gccccggctg gacctgttga tgctcggggc gccggaccgg
     1861 agccagccgc tcgactactt cgtgacggcg gggtcgctgg cggagcccgc ggaccacgcg
     1921 ggagtcgtga cggtgggtgc ggagaacccg ttcgacaacg cgctggtggc gaacttctcc
     1981 tcggtcgggc cgacgca
//
LOCUS       DAORPG010000393         1994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7164_length_1994_cov_2.731821, whole genome shotgun sequence.
ACCESSION   DAORPG010000393 DAORPG010000000
VERSION     DAORPG010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7164_length_1994_cov_2.731821"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..1785
                     /locus_tag="PLX85_08340"
     CDS             184..1785
                     /locus_tag="PLX85_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HRC63211.1"
                     /translation="MQPLTEEQRSEIELLLATLPARVATAVRTHGLEGLLEIVLDFGR
                     VPTARYAGSAEVILDEREVSEQDLDHVVAQISEFAEDNRAGIPRTLHRISAIRNRRGR
                     IVGLTCRVGRSVSGSASVIRDLIETGRSVLLLGPPGVGKTTMLREAARLLADELHKRV
                     VIVDTSNEIGGDGDIPHHGVGRARRMQVPRPELQHQVMIEAVENHMPEVIVIDEIGTA
                     LEALAARTIAERGVQLIGTAHGNALSNLMQNPTLSDLIGGIESVTLSDEEARRRGTQK
                     TVLERRAQPTFDSLVEIQSFDRVAVHEDIASTVDALLRGFDAEAEIRVLDAAGEVVEV
                     ERVPLAGQGEERFDDIEERDRPRRIMPAPGGPQRRILPFGISRTRLEEAIANSRSAAV
                     VVDSIQDADAVMTLRPYYRRRTGPLRDAEARGIPIYVLRNNTVGQIEQSLLSLRGASG
                     ELDPMTAALQEAEEAIAAVSFEGKSAVELAPQNAYIRRLQHELASRHGVETSSRGREP
                     FRRVLVVASNGRQLREDAWADDLRA"
     gene            1782..>1994
                     /locus_tag="PLX85_08345"
     CDS             1782..>1994
                     /locus_tag="PLX85_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63212.1"
                     /translation="MTASGRFVTLEGGEGAGKSTQLEALAALARDAGIEVVTCREPGG
                     TVLGERLRAALFDLERAPEPLAELLVF"
BASE COUNT          307 a          761 c          653 g          273 t
ORIGIN      
        1 aaccagactg cctcggcacg cccgccgcgt tcctgcgcga ccttggcttc gagccgcgcg
       61 atcgctacgt gctgctcgcg aaacggctcg cccgacccat cttcgaggca atcccgagcg
      121 cagccggaag gacggtgccc actcgtggct gagcccgcca gcattcgtcc cagcgacggc
      181 cacatgcagc cgctgaccga ggagcagcgc agcgagatcg agctgctgct cgccacgctg
      241 cccgcccgcg tcgcgaccgc cgtgcgcacg cacggcctcg aagggctact ggagatcgtg
      301 ctcgacttcg gccgcgtgcc gaccgcacgg tacgccggca gcgcggaggt catcctcgat
      361 gagcgcgaag tgagcgagca ggacctcgac cacgtcgttg cgcaaatctc ggagttcgcg
      421 gaggacaacc gcgccggcat cccgcgcacg ttgcatcgca tctccgcgat ccgtaaccgc
      481 cgagggcgca tcgtgggcct cacctgccgc gttggccgca gcgtgagcgg gtccgcgagt
      541 gtgatccgcg acctcatcga gaccggccgc agcgtgctcc tcctcggccc gccgggcgtc
      601 ggcaaaacca cgatgctccg cgaggccgcg cgactccttg ccgacgagct gcacaagcgc
      661 gtcgtgattg tcgacacctc gaacgagatc ggcggcgacg gcgacatccc ccaccacggc
      721 gtgggacgcg cgcgccgcat gcaggtgccg cgccccgagc tgcagcacca ggtcatgatc
      781 gaggccgtcg aaaaccacat gcccgaggtc atcgtcatcg atgagatcgg cacggccctc
      841 gaggcgctcg cggcccgcac gatcgccgag cgcggcgtcc agctcatcgg caccgcccac
      901 ggcaacgcac tctcgaacct catgcagaac ccgacacttt ccgatctcat cggcggcatc
      961 gagtcggtca cgctgtccga cgaggaggcg cgccgccgcg gcacgcagaa gaccgtcctc
     1021 gagcgccgcg cacagccgac attcgactcc ctcgtcgaaa tccagagctt cgaccgtgtc
     1081 gccgtgcatg aggacatcgc ctccacggtc gacgcgctgc tgcgcggctt cgacgccgag
     1141 gctgagatcc gcgtcctcga cgccgccggg gaggtcgtcg aggtggaacg cgtccctctc
     1201 gccggccagg gcgaggagcg cttcgacgac atcgaggagc gagaccgtcc tcgtcgcatc
     1261 atgcccgcgc cgggcggccc gcagcgtcgc attctcccgt ttgggatcag ccgtacgcgc
     1321 ctcgaggagg cgatcgcgaa ctcgcggtcg gccgcggttg tcgtcgacag catccaggac
     1381 gccgacgccg tgatgacgtt gcgcccgtac taccgtcgcc gcaccggccc actccgggac
     1441 gccgaggcac gcggaatccc gatctacgtg ctgcgcaaca acaccgtggg ccagatcgag
     1501 cagtcgctgc tcagcctgcg cggcgcctca ggcgagctcg acccgatgac cgccgcgctg
     1561 caggaggccg aggaggccat cgcggccgtc tccttcgagg gcaagtcggc cgtcgagctg
     1621 gcgcctcaga acgcctacat ccgccgcctc cagcacgagc tggccagccg ccacggcgtc
     1681 gagacctcct cgcgcggccg cgagccgttc cgccgcgtcc tcgtggtcgc ctcgaacggg
     1741 cgccagctgc gtgaggacgc ctgggccgac gacctccgcg catgaccgct tccggccgct
     1801 tcgtcaccct cgagggcgga gagggcgccg gcaagtccac ccagctcgag gcgctggccg
     1861 ccctcgctcg cgatgccggc atcgaggtcg tcacctgccg cgagcccggc ggcacggtcc
     1921 tcggtgagcg cctgcgcgcg gcgctgtttg acctcgaacg cgcccccgag ccgctggccg
     1981 agctgctcgt cttc
//
LOCUS       DAORPG010000394         1992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7179_length_1992_cov_3.073309, whole genome shotgun sequence.
ACCESSION   DAORPG010000394 DAORPG010000000
VERSION     DAORPG010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7179_length_1992_cov_3.073309"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLX85_08350"
     CDS             complement(<1..345)
                     /locus_tag="PLX85_08350"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRC63213.1"
                     /translation="MRATIFEAPGKVSVVDRATPEPGPGELRLRMAAASLCASDVRVY
                     RGEKYAKPGVVPGHEIAGVVEAVGEGAGFAEGDRVVVCPVVACGHCRFCIVGRQNRCV
                     DRRTLGYDLDGGF"
     gene            375..653
                     /locus_tag="PLX85_08355"
     CDS             375..653
                     /locus_tag="PLX85_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63214.1"
                     /translation="MASIAVRRLAAPAKGGAAYPHSLPVPLPPCFPAPYTRRTGNDGD
                     STVGRPPSRRGLRSESGTVQAGRSHPDQNITPEPPPEREAPRRQVAAR"
     gene            959..>1992
                     /locus_tag="PLX85_08360"
     CDS             959..>1992
                     /locus_tag="PLX85_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRC63215.1"
                     /translation="MTVFDPIPSRVSFPALEHDVLAFWRDHDVFRRSVDERPEDRVFS
                     FYEGPPTANGNPGIHHVLARVFKDLIPRYKTMRGYRVPRKGGWDTHGLPVELEVEKQL
                     GLRSKPDIEVYGIEEFNRKCRESVFRYVEQWERMTERIGFWVDLDDAYVTYSRDYVET
                     CWWIFKRLWDSDLMFRDYRVTPHCPRCGTSLSSHEIALGYKEDTPDPSVTVRFRLLPE
                     TNPEAPAAPVVEAVLRTAAPDAKPTALLAWTTTPWTLPGNVALAVSPDADYVVVARED
                     EQLILAKDRVAAVVGEDAQVVAAFKGTDLVGLRYEPLYESREWDGVEALVFLDGRAQR
                     AEAASEPRARR"
BASE COUNT          298 a          795 c          624 g          275 t
ORIGIN      
        1 gaacccgccg tcgaggtcgt agcccagcgt gcggcggtcc acgcagcgat tctgccgccc
       61 cacgatgcag aaacggcagt ggccgcacgc gacgacagga cagacgacca cccggtcgcc
      121 ctcggcgaac ccggcccctt cgcccacagc ctcgacgacg cccgcaatct cgtgtcccgg
      181 caccacgccg ggcttcgcgt acttctcgcc tcgatacacc cgcacgtccg aggcgcacag
      241 cgacgcggcc gccatgcgca gccgcagctc gcccggcccc ggttcgggcg tcgcccggtc
      301 caccaccgat accttgcccg gggcttcgaa gatggtcgcg cgcatgctca ctccgtcgtc
      361 gctgtgccga ggtcatggcc agtatcgcag tgcgccgcct cgccgcgcca gctaagggcg
      421 gcgccgccta cccacactcc ttgcccgtgc cgttgccacc ctgcttcccc gccccctaca
      481 ctcggaggac aggcaacgac ggagacagta cggtcggccg cccgccctcg aggcgggggc
      541 tgcgcagcga gtccgggacg gtgcaagccg gacgcagcca ccccgaccag aacatcactc
      601 ccgagccgcc gcccgaacgc gaagcccctc gacggcaggt cgccgcgagg tgacccgcca
      661 gtagacgcgg ccggagcgcc caccgttacc aggggcagcg ccatcgaggc ggcttcgagc
      721 ctccgtggcg cggtgagcgg cgcgcgcacc tcgcaacgtt cgaggcagcg gcgcaacgcg
      781 ggtggtaccg cgggccgcgc gattcgtatc gctccgcccg tcccgcaggg ggcgggcttc
      841 ttcgtgccct ccacggggcg ccccgagccc ggtcccacgc actcgtcccc tcgcccgcac
      901 gcgagacgcg cgtgtgaggg gacaggagtg agggcgtcgc cctcggaagg tacgagccat
      961 gaccgtcttc gaccccattc cctcgcgcgt ctcgttcccc gccctcgagc acgacgtcct
     1021 cgccttctgg cgcgaccacg acgtcttccg ccgcagcgtc gacgagcgtc ccgaggaccg
     1081 cgtgttctcc ttctacgagg gcccgcccac cgccaacggc aacccgggca tccaccacgt
     1141 gctcgcgcgg gtcttcaagg acctcatccc ccgctacaag accatgcggg gctaccgcgt
     1201 gcctcgaaag ggcggctggg acacccacgg cctgccggtg gagctcgagg tcgagaagca
     1261 gctcggcctc cgctccaagc cggacatcga ggtctacggc atcgaggagt tcaaccggaa
     1321 gtgccgcgag tccgtcttcc gctacgtcga gcagtgggag cgcatgaccg aacgcatcgg
     1381 cttctgggtg gacctcgatg acgcctacgt cacctactcg cgcgactacg tcgagacctg
     1441 ctggtggatc ttcaagcgcc tgtgggacag cgacctcatg ttccgtgact accgagtcac
     1501 cccgcactgc ccgcgctgtg gcacctcgct ctcctcgcac gagatcgcgc tcggctacaa
     1561 ggaggacacc cccgacccca gtgtgaccgt gcgcttccgc ctcctccccg agacgaaccc
     1621 cgaggcaccc gccgccccgg tcgtcgaggc cgtgcttcgc accgccgctc ccgacgcgaa
     1681 gcccaccgcg ctcctcgcct ggacgaccac gccgtggacg ctccccggca acgtcgccct
     1741 cgccgtctcg cccgacgccg actacgtcgt cgtggcccgc gaggacgaac agctgatcct
     1801 cgcgaaggac cgcgtcgccg ccgtcgtcgg cgaggatgcc caggtggttg ccgccttcaa
     1861 gggcaccgac ctcgtcggcc tgcgctatga gcccctctac gagtcacgcg aatgggacgg
     1921 cgtcgaggcg ctcgtgtttc tcgatggccg cgctcagcgc gccgaagccg cctccgagcc
     1981 gcgtgcgcgc cg
//
LOCUS       DAORPG010000395         1990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7194_length_1990_cov_3.261499, whole genome shotgun sequence.
ACCESSION   DAORPG010000395 DAORPG010000000
VERSION     DAORPG010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7194_length_1990_cov_3.261499"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PLX85_08365"
     CDS             <1..764
                     /locus_tag="PLX85_08365"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HRC63216.1"
                     /translation="GHEVHLVRLAFGANAERDAATFGSLDFAASSGSPVHAGAVPVAG
                     GITIVDASVSPDAASLAQQLGATRIEVSGDEDRAGTASVMRVMNHARAAGPLAIPEDR
                     LLAAPTVGTLIEASRARVLARSVEGERAICEHVVVGAISHDSDQPYFARFPHKAVVTR
                     AERVDIALSALRTDADCLILTGGSEPSPYLLDRVAASRRTTLLVTPEGTVETVRDIEG
                     MFGRSPFGGDAKIERIGLLMASALDDEALASLTGQ"
     gene            812..1936
                     /locus_tag="PLX85_08370"
     CDS             812..1936
                     /locus_tag="PLX85_08370"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRC63217.1"
                     /translation="MNLGAGIGIGRIRGIEIRIHWSWFIILVLLTWSFADGLYRDLYE
                     NWSDSQRWTAAAVTSGLFFLSVLLHELSHAFVAQRYGMSVPSITLFVFGGVSSIAGEM
                     RTAREEFAVAIAGPLMSWVLAVIFGVLWLATHGTGLAGIFGYLAWINGALGLFNLLPG
                     FPLDGGRVLRSIVWGRSKSLVRATRVAASVGSIIAYLMIALGLLSVFYFGLLGGIWYV
                     LIGLFLKSAAEGSYQAMLADRALRNVDTRTVMQPPSQPVDPSMTVQQLVDERLLRAAE
                     RAYLVGDEGRVLGLVTASDITRLPRERWASTTVRDVMVPAEQVKTVTPQTDLVAAMQL
                     MQQHDVHQLPVLEDGRLVGMLTRGDVLRQIEIRMRFQDGT"
BASE COUNT          270 a          632 c          726 g          362 t
ORIGIN      
        1 cggggcacga ggttcacctc gtgcggctcg ccttcggcgc aaacgcggaa cgggacgccg
       61 ccacctttgg atcgctcgac ttcgccgcga gttccggttc gccggtccac gcgggcgccg
      121 tgccggtcgc aggggggatc acgattgtcg acgcctccgt ttctccggac gccgccagcc
      181 tggcgcagca gctgggcgcc acgcgtatcg aggtctcggg cgacgaggac agggccggta
      241 ccgcctcggt catgcgcgtc atgaaccacg ctcgggcggc ggggccgttg gcaatcccgg
      301 aggaccgcct gctggccgcg ccgaccgtgg gcacgctgat cgaggcgagc cgggcacggg
      361 tgcttgctcg ctcggttgag ggagagcggg cgatctgcga gcacgtcgtc gttggcgcga
      421 tctcgcacga ttcggatcag ccctacttcg ctcgctttcc gcacaaggcg gtcgtgacgc
      481 gggcggagcg tgtggacatc gcgctgtccg cgctcaggac tgatgccgat tgcctgatcc
      541 tcacgggtgg cagtgaaccc agtccgtatc tgctcgatcg ggtggcggcg agccggcgca
      601 cgacgctcct cgtgaccccc gaggggaccg ttgagactgt gcgtgacatc gaggggatgt
      661 tcggccgttc gccgttcggc ggcgacgcga aaatcgagcg gatcgggctc ctgatggcca
      721 gtgcgctcga tgacgaggcg ctggcgtcgc tgaccggcca gtaaggcctc ggtaccgctt
      781 cgatccgggg ccggcggggt atgctggagg catgaacctc ggcgctggaa tcggcatcgg
      841 acgcatccgc gggatcgaaa tccgcatcca ctggtcgtgg ttcatcatcc tcgtcctgct
      901 gacgtggtca ttcgccgacg ggctctaccg cgacctgtac gagaactggt cggatagcca
      961 gcgctggacc gccgcggccg tcacgagtgg cctgttcttc ctgtctgtgc tcctgcacga
     1021 gctgtcgcac gcctttgtcg cgcagcggta tgggatgtcg gtgccctcta tcacgctgtt
     1081 cgtgtttggc ggggtgtcga gcatcgccgg ggagatgcgg accgcacgcg aggagttcgc
     1141 ggtcgcgatc gctggcccgc tcatgtcgtg ggtgctcgcg gtgatcttcg gggtgctgtg
     1201 gctggcgacg cacggcacgg gcctggcggg tatcttcggc tatctcgcct ggatcaacgg
     1261 agcgctcggc ctgttcaacc tgctgcccgg cttcccgctc gatggcggcc gagtgctgcg
     1321 ttcgatcgtg tgggggcgct cgaagagcct ggtgcgggcg acgcgagtgg cggcctcagt
     1381 gggctccatc atcgcgtatc tgatgatcgc gctgggcttg ttgagcgtct tctacttcgg
     1441 actgctgggc gggatctggt acgtgctcat cgggctcttc ctgaagtcgg cggcggaggg
     1501 gtcgtaccag gcgatgctcg ccgaccgggc gctgcgcaac gtggacacgc gcacggtcat
     1561 gcagccgccc tcgcagccgg tggacccttc gatgactgtg cagcagctcg tcgacgaacg
     1621 tctgctacgg gccgccgagc gggcatacct cgtgggcgat gaggggcggg tgctcggcct
     1681 cgtgacggcg agcgacatca cccgtctgcc ccgcgagcgg tgggcgagca cgaccgtgcg
     1741 ggacgtgatg gtcccggcgg agcaggtgaa gacggttacg ccgcagaccg acctcgtcgc
     1801 ggcgatgcag ctgatgcagc aacacgacgt ccaccagctg ccggtgctcg aggatggccg
     1861 gctcgtcggc atgctgacgc gcggcgatgt gcttcgccag atcgagatac ggatgcgctt
     1921 ccaggacggg acgtagccct caggcgggct cccactggtc atcgacctcg cgaggggctt
     1981 ccgcctcgag
//
LOCUS       DAORPG010000396         1988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7202_length_1988_cov_4.612519, whole genome shotgun sequence.
ACCESSION   DAORPG010000396 DAORPG010000000
VERSION     DAORPG010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7202_length_1988_cov_4.612519"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLX85_08375"
     CDS             <1..409
                     /locus_tag="PLX85_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRC63218.1"
                     /translation="KVGVIGSCSGGRHTYLAVCQTSEFDAAVDLWGGRVVMRPDELNE
                     RTPVAPIDLTKDLSCPLLGLFGAEDRSPTAEQVAQHEAALKEHGKDYEFHMYEGAGHG
                     FFYYHSGMYRQEAAVDGWEKVWAFFAKHLGGPA"
     gene            490..1245
                     /locus_tag="PLX85_08380"
     CDS             490..1245
                     /locus_tag="PLX85_08380"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRC63219.1"
                     /translation="MFTTDQYQGMLAETVTYAGANGDVIDAYLARPLGPGPFPSVVLI
                     HNVLGWGEYYHEMTRKFAHHGYVAICPNLFDRDGDGTPEDVAAQVRADGGPPDDRVIG
                     DVEGAAAFVRSLPYTSGKVGVIGSCSGGRHTFLAACRTSAFDAAVDLWGGRVVMAPEE
                     LTERTPVSPIEYTKDLSCPLLGLFGVEDRNPTPDMVAQLEAALKEHGKQYEFHSYEGA
                     GHGFFYYHMGMYRQEQAVDGWEKVFAFFEQHLS"
     gene            1307..1570
                     /locus_tag="PLX85_08385"
     CDS             1307..1570
                     /locus_tag="PLX85_08385"
                     /inference="COORDINATES: protein motif:HMM:NF039404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6295 family protein"
                     /protein_id="HRC63220.1"
                     /translation="MCTMIVEKVSVTGVAKGGGNWFPIARVNVGYDHPFRLPLEHAVT
                     LDFVNEAQGPGARVAVELTPQSAQQLLDTLRDVLEQGAAAEAA"
     gene            complement(1660..>1988)
                     /locus_tag="PLX85_08390"
     CDS             complement(1660..>1988)
                     /locus_tag="PLX85_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRC63221.1"
                     /translation="AAASTLASVGIEARVLNMASVRPLDTAALEAAAGETGAIVVAEE
                     HLTHSGLGAMCAQHLAMHRPVPMEFVGVQNRYGESGTSNELLEIMGLTPEGIVAATKR
                     VLTRKG"
BASE COUNT          308 a          656 c          724 g          300 t
ORIGIN      
        1 gaaggtcggg gtgatcggaa gctgctccgg cgggcggcat acctacctgg cggtctgcca
       61 gacctcggag ttcgatgcgg cggtcgacct gtggggcgga cgcgtggtga tgcgccccga
      121 tgagctgaac gagcgaacgc cggtcgcgcc gatcgacctc acgaaggacc tctcgtgccc
      181 gctgctcgga ctgttcggcg cggaggaccg cagcccgacc gcggagcagg tcgcgcagca
      241 cgaggcggcg ctcaaagaac acggcaagga ctacgagttc cacatgtacg agggcgccgg
      301 gcacggcttc ttctactacc actccggcat gtaccggcag gaggcggcgg tcgacggctg
      361 ggagaaggtg tgggcgttct tcgcgaagca cctcggaggc ccggcgtagc tggcggcgcg
      421 cccacgccgt ccggctgaag gtggtacaac tcacacgttt gacgagtctg agcgcgtgca
      481 ggaggaagca tgttcacgac tgaccagtac cagggaatgc tcgccgagac ggtcacgtac
      541 gcgggtgcga acggagacgt gatcgacgcg tacctcgcgc ggccgctcgg gcccggcccg
      601 ttcccgtccg tcgtcctgat ccacaacgtc ctgggctggg gcgagtacta ccacgagatg
      661 acgcgcaagt tcgcgcacca cgggtacgtt gccatctgcc cgaacctctt cgaccgcgac
      721 ggcgacggca cccccgagga cgtcgcggcg caggtgcgcg ccgacggcgg cccgccagac
      781 gaccgcgtga tcggagatgt cgagggcgcg gctgccttcg tacgctcgct gccctacacg
      841 agcggcaagg tgggcgtcat cggcagctgc tcgggcggcc ggcatacgtt cctcgctgcc
      901 tgccgcacct cggcgttcga cgcggcggtg gacctctggg gtgggcgcgt ggtgatggcg
      961 ccggaggagc tgacggagcg gacgcccgtc tcccccattg agtacacgaa ggacctctcg
     1021 tgcccgctcc tcgggctgtt cggcgtcgag gaccgcaacc cgacgccgga catggtcgcc
     1081 cagctcgagg cggccctgaa ggagcacggc aagcaatacg agttccacag ctacgagggc
     1141 gccgggcacg gcttcttcta ctaccacatg gggatgtacc ggcaggagca ggccgtcgac
     1201 ggctgggaga aggtcttcgc cttcttcgaa cagcatctgt cgtaaccgct gcggaccccg
     1261 cggccccgga cctcgagaca tccgacggag atcaggagca atcgcaatgt gcacgatgat
     1321 cgtggagaag gtatcggtga cgggcgtggc aaagggcggc gggaactggt tcccgatcgc
     1381 gcgggtgaac gtcggctacg accacccgtt ccggctgccg ctggagcatg ccgtgacgct
     1441 cgacttcgtg aacgaggccc agggccccgg cgctcgcgtg gccgttgagc tgacgccgca
     1501 gtcggcgcag cagctcctcg acaccctgcg cgacgtgctg gagcagggcg ccgccgccga
     1561 ggcggcctga ggcgttgacc gggtgggggc gtcgaggact cctcggcgcc ctcttcgatg
     1621 gcgggtcagc cgggcagcgg cacctcggtg tcgcggtagt taccccttgc gggtcagcac
     1681 ccgcttcgtt gccgcgacga tgccctcggg ggtcaggccc atgatctcga ggagttcgtt
     1741 gctcgtgccg gattcgccgt agcggttctg gacgcccacg aactccatcg gcacgggccg
     1801 gtgcatcgcg aggtgctgcg cgcacatcgc gccgaggccc gagtgcgtca ggtgctcctc
     1861 ggccacgacg atggcgccgg tctcgcccgc ggcggcctcg agggcggccg tgtcgagggg
     1921 gcggacgctg gccatgttga gcacgcgcgc ctcgatgcct acgctcgcga gggtcgaggc
     1981 ggcggcca
//
LOCUS       DAORPG010000397         1987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7209_length_1987_cov_3.880435, whole genome shotgun sequence.
ACCESSION   DAORPG010000397 DAORPG010000000
VERSION     DAORPG010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7209_length_1987_cov_3.880435"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1035
                     /locus_tag="PLX85_08395"
     CDS             217..1035
                     /locus_tag="PLX85_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRC63222.1"
                     /translation="MRLEPPHPLVGLAVASVLALALLITRPAPSAAAAGTLQGDIPHD
                     GTGLVVWGGGTTTELSQAAEQQGCHTTTLWTTSSGHFAVYIYGAPDVVNAPFIEQFPG
                     TSMPPGTALVIACSGPVGPSQSAIVAISSIENEFANAAFDGINRARVENGRSTLILDT
                     RLRSAAEKYASLVFSKGELSHSFDGQPWERAQREGFPSQYVGEVIVSRGSSEGLNVPH
                     DAPELVTAWMNSPPHRDIIIGKDYSFVALGVGCATGIDRAGLNTVVCVAMLGTP"
     gene            complement(1057..>1987)
                     /locus_tag="PLX85_08400"
     CDS             complement(1057..>1987)
                     /locus_tag="PLX85_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020731107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HRC63223.1"
                     /translation="FERDDRIGGLLRYGIPDFKMQKEFIDRRLRQMEAEGVEFRTSVN
                     VGEDIDARDLLQQFDAVCLTGGATHARDLEVPGRDLQGVYLAMEYLPLQNRRNAGDEI
                     PEGQFISAKDKRVVILGGGDTGADCLGTAIRQGATEVQQYELLPRPPDSRPSDTPWPT
                     WPNIYRVSSAHEEGGVRDYSILTKSLSGEGGVLKKLHAVRVEFVPEDGRQVMREVPGS
                     EFEVEVDLLLLAMGFLGPQRPGMLEQLGVRLTERGNVWADENRMTSVPGVFTAGDMTR
                     GQSLIVWAIAEGRTAARGIDAYLMESSALPAPL"
BASE COUNT          317 a          652 c          665 g          353 t
ORIGIN      
        1 tcgatctgga tcaccgcgca ggggcaattc gtcccgtatg tcgtcggcgc gcccgacttc
       61 gtgaatgacg gcttcgtggg ccgcttcacg ggtgggaaca tgccgagcgg cactccggtc
      121 atcattgtct gccagggagg gtagcgggta gacgatgcgg cgcttgcgcg ccaccataca
      181 atcctgtcat tggcataacg tggaagtggt gagctcatgc gcctcgagcc cccacacccg
      241 ctcgtcgggt tggctgttgc gtccgtcctc gcgcttgcgc tgttgattac tcgtccggcg
      301 ccgtcggcgg cggccgccgg caccctccag ggggacatcc cacacgacgg gacgggcctc
      361 gtcgtgtggg gcggcggcac taccaccgag ttgagtcagg cggccgagca gcaggggtgt
      421 cacacgacca cgctgtggac gaccagcagc ggtcattttg ccgtgtatat ctacggcgcg
      481 ccggacgtgg tcaacgcacc gttcatcgaa cagttccccg gaacctcgat gccgccgggc
      541 acggcactcg tcatcgcatg cagtggccct gtcggcccct cgcagtcggc gatcgtggct
      601 atctcatcga tcgagaacga gttcgcgaac gccgctttcg acggcatcaa ccgggcccga
      661 gtcgagaatg gccgttcgac gctcattctc gacacccgtc tgcggtcggc tgctgagaag
      721 tacgcgagcc ttgtcttctc caagggggaa ttgagccact ccttcgacgg gcagccgtgg
      781 gaacgtgcgc agcgcgaagg gttcccgagc cagtatgtcg gcgaggtcat cgtctcgcgt
      841 ggatcgtccg agggcctgaa cgtcccgcat gacgccccgg agctcgtcac ggcctggatg
      901 aacagtcccc ctcaccgcga catcatcatc ggcaaggatt actcgttcgt cgccctcggg
      961 gtcggttgtg caacgggcat cgaccgagcc ggactcaaca cagtggtctg cgtcgcgatg
     1021 ctcggaacgc cctgaaggaa atcgctcggg cgctgactac aatggcgcgg gtagcgcact
     1081 cgattccatg aggtaggcat cgatgccgcg ggccgccgtg cggccctcgg cgatcgccca
     1141 cacgatcaac gactgcccgc gggtcatgtc gccggcggtg aagacaccgg gcacgctcgt
     1201 catccggttc tcgtcggccc agacgttgcc gcgctcggtc aggcgcaccc caagctgttc
     1261 aagcatgccg ggccgctgcg gaccgaggaa gcccatcgcg aggagcagga ggtcgacttc
     1321 gacctcgaac tcggagccgg gaacctcccg catcacctgg cggccatcct cggggacgaa
     1381 ctcgacgcgg acggcgtgca gcttcttgag gaccccgccc tcacctgaaa ggctcttggt
     1441 caggatgctg tagtcgcgga cgccgccctc ctcgtgcgcc gaggagacac ggtagatgtt
     1501 cggccaggtg ggccagggcg tatccgatgg ccgcgagtcg ggtggccgcg gcagcaactc
     1561 gtactgctgc acctccgtcg cgccctgccg gatggcggtg cccaggcagt ccgcgccggt
     1621 atcgccacca ccgaggatga cgacgcgctt gtccttcgcg ctgatgaact ggccttccgg
     1681 gatctcgtcg cccgcgttgc ggcggttctg cagcggcagg tattccatcg cgaggtagac
     1741 gccttgcagg tcgcgtcccg ggacttcgag gtcccgagcg tgcgtggcgc cgccggtcag
     1801 gcagaccgcg tcgaactgct ggagcagatc gcgggcgtca atgtcctcgc cgacgttgac
     1861 ggaggtgcgg aactcgacgc cttcggcttc catctggcga aggcgacggt cgatgaattc
     1921 cttttgcatc ttgaagtcgg ggatgccgta gcggaggagg ccgccgatgc ggtcgtcgcg
     1981 ctcgaag
//
LOCUS       DAORPG010000398         1975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7291_length_1975_cov_2.769792, whole genome shotgun sequence.
ACCESSION   DAORPG010000398 DAORPG010000000
VERSION     DAORPG010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7291_length_1975_cov_2.769792"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PLX85_08405"
     CDS             <1..205
                     /locus_tag="PLX85_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63224.1"
                     /translation="VCSLPNATPACTNGSCSIASCANNFANCDGVAGNGCEINTQIDP
                     THCGNCATVCSVANGTPGCAAGA"
     gene            222..545
                     /locus_tag="PLX85_08410"
     CDS             222..545
                     /locus_tag="PLX85_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRC63225.1"
                     /translation="MAKLSPAVVNVATERPVQQTARDPKVPPGFQRSGVDSEARSLGS
                     GFVIDPSGLIVTNNHVIDGASEIFVTLRDGTRRAATVRGRGRSPAVPSPTTRSISLTS
                     SASRP"
     gene            509..1165
                     /locus_tag="PLX85_08415"
     CDS             509..1165
                     /locus_tag="PLX85_08415"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRC63226.1"
                     /translation="MYLVDFLRFTTVTPEEVRERVVFDEWDRLEAQRAGQGIVFVTMH
                     FGNWDLGAAALAEHGIPISVVADTFGDPRLNDLVLGARRHLGMEILAAERMGPSILRS
                     LHRNNVVAMLIDVPQEGPGLEVQFFDGPVAVADGPARIALRTGAPVVAVLLPRIGRSE
                     RVLGRIAPVAFVPSGEQERDVRELTQATMTALEAMLREHPDQWYIFRNLWVADRKGGR
                     "
     gene            1169..>1975
                     /locus_tag="PLX85_08420"
     CDS             1169..>1975
                     /locus_tag="PLX85_08420"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63227.1"
                     /translation="MLRYAWLWFLVRVVGRAPDWFLMAAADLGGMLLWYASPRVRAVT
                     TDHMRHVLGTLSTANERTRAAKGCVRTAGRYYADFCRAPHRPTGAAFDELDAVEGLDR
                     WFEALDRGCGLIVCSAHLGNPEFFVRAVGQLEIDLLVLTEPLSPPRVHRLVHEARAAR
                     GVRFMPAGLSAVREAITHLKQGGVLAVLGDRDVLHNGRPTVFFDERARLPSGAVDLSL
                     RTGAPILSVFVTRTPRGRYRVYIDEAFQVQRTGDHEADIEAGMRQVAAALE"
BASE COUNT          275 a          660 c          721 g          319 t
ORIGIN      
        1 cgtgtgcagc ctgccgaacg cgacccccgc ttgcaccaac ggctcctgca gcatcgcctc
       61 ctgcgccaac aacttcgcca actgcgacgg cgtcgctggc aacggctgcg agatcaacac
      121 gcagatcgat cccacccact gcggcaattg cgccaccgtg tgcagcgtcg ccaacggcac
      181 gcccggctgc gcggccggcg cctgagggtt ttgccgaact ggtggcgaag ctttcgcccg
      241 ctgtcgtcaa tgtggcgacg gagcggccgg tgcagcagac ggcgcgcgat cccaaggtgc
      301 cgccagggtt tcaacgcagc ggcgtcgata gcgaggcgcg ctcgctcggc tccgggttcg
      361 tgatcgaccc gtccgggctg atcgttacga acaatcacgt gatcgacggg gcaagcgaga
      421 ttttcgtgac gctgcgtgac ggaacgcgcc gggcggcgac ggtgcgcggg cgcggacgct
      481 cgcccgccgt tccttctcca actacgcgat ctatctcgtt gacttcctcc gcttcacgac
      541 cgtgacgccc gaagaggtgc gcgagcgcgt ggtgttcgac gagtgggatc gcctcgaggc
      601 gcagcgcgcg gggcagggca tcgtcttcgt gacgatgcac ttcgggaact gggacctcgg
      661 cgcggcggcg cttgccgagc acggcatccc gatctcggtc gtggcggaca cgttcggcga
      721 tccgaggttg aacgacctgg tactcggagc gcgccggcac ctgggcatgg agatcctggc
      781 ggcggagcgg atggggccga gcatcctgcg ctcgctgcac cgcaacaacg tggtcgcgat
      841 gctgatcgat gtgccgcaag aggggccggg cctggaagtg cagtttttcg atggccccgt
      901 cgccgtcgcg gacggtccgg cacggatcgc gctgcgcacg ggcgcgccgg tggtggcggt
      961 gctgctgcct cgcatcggcc ggagcgagcg agtgttgggg cggatcgccc cggtcgcgtt
     1021 tgtcccgagt ggcgagcagg agcgggacgt gcgcgagctg acccaggcga caatgacagc
     1081 actcgaggcg atgctgcgcg agcatcccga tcagtggtac atcttccgca acctctgggt
     1141 agccgaccgg aagggcgggc ggtagtccgt gctccgctac gcgtggctct ggttcctggt
     1201 ccgcgtggta gggcgggcgc ctgactggtt tctgatggcc gccgccgacc tcggcggaat
     1261 gctgctgtgg tacgcgtcgc cccgggtgcg ggcggtgaca accgaccaca tgcgccacgt
     1321 cctcggcacg ttgagcactg cgaacgagcg aacgcgggcc gcgaaggggt gcgtgcgcac
     1381 ggcgggccgc tactatgccg acttctgccg cgcgccgcac cggcccaccg gcgccgcctt
     1441 cgatgagctc gatgccgtcg aggggctcga ccgctggttc gaggcgctcg accgcggctg
     1501 cggcctgatc gtgtgctccg cgcacctcgg caatcccgag ttcttcgtgc gggcagtcgg
     1561 ccagctggag atcgacctgc tcgtgctgac ggagccgctc tcgccgcctc gcgtgcaccg
     1621 cctggtgcac gaggcgcgcg ctgcacgcgg ggtgcgcttc atgccggctg ggctgagcgc
     1681 cgtgcgcgag gcgatcacgc atctgaagca gggcggcgtg cttgcggtcc tcggcgaccg
     1741 ggacgtgctg cacaacgggc gaccgacggt gttcttcgac gagcgtgccc gtctgccgag
     1801 cggcgcggtc gacctctcgt tgcgcaccgg cgctccgatc ctgtcggtct tcgtcacccg
     1861 gacgcctcgc gggcgctacc gcgtgtacat cgacgaggca ttccaggtgc agcggaccgg
     1921 tgaccacgag gcggacatcg aggcggggat gcgacaggtg gccgcggcgc tcgag
//
LOCUS       DAORPG010000399         1965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7352_length_1965_cov_3.197906, whole genome shotgun sequence.
ACCESSION   DAORPG010000399 DAORPG010000000
VERSION     DAORPG010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7352_length_1965_cov_3.197906"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PLX85_08425"
     CDS             complement(<1..626)
                     /locus_tag="PLX85_08425"
                     /inference="COORDINATES: protein motif:HMM:NF014749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRC63228.1"
                     /translation="MQSRHLTSILDLSPEDLERVLDVALSIKRDGTGPALSGQVLALL
                     FEKPSLRTRVSFEVAMSRLGGRSLYIGKEEVQIGSREPVRDVARVLSRMVNGVAIRTF
                     DQAPLDEFASWSSVPVINALSDHEHPCQVLADLLTLRERFGTLRGLRLAYLGEGNNVA
                     RSLAYAAVLAGVEFVCASPAGYALTEVDIGQASELPGGGSVLQTDDAVA"
     gene            757..1278
                     /locus_tag="PLX85_08430"
     CDS             757..1278
                     /locus_tag="PLX85_08430"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HRC63229.1"
                     /translation="MANHRWLNAALAASALLAFLVLTFPNDSDDLGIEVESRDPSPGI
                     DEIRVAVTGAVARPGVVTVRPGDRVADAIALAGGLTADGDMNAVNLARRVRDEDQVVV
                     PRVGERFALLDLNRATAQELEALPGVGPTYANAIVQARTTHGPFESTDQLVELGLVPE
                     RVYERFRDLVTVH"
     gene            1278..>1965
                     /locus_tag="PLX85_08435"
     CDS             1278..>1965
                     /locus_tag="PLX85_08435"
                     /inference="COORDINATES: protein motif:HMM:NF024956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRC63230.1"
                     /translation="MPALPVVTGAFVAGALLGALLGGPVAWTMLLVATTVIAVTLRSR
                     EHLSWLVLIAAVAFAGVGHLRALSAAESQQRSEAIEGTHVLSGVVESPPDVRGTTRFF
                     SLLVDEVDGAPRHIRVRVIVPATGQVRLGDRVELSGELESPRDGASPGARQRGNDLGV
                     EAEVAFPSRWRVIGHEPRSRIDSVLGALHERSIANIERVLREPESSLAVGMLLGVQQA
                     MPERLTRDLRA"
BASE COUNT          325 a          681 c          648 g          311 t
ORIGIN      
        1 gcgacggcat catcggtctg cagtacagat ccgccacccg gcagctcgga cgcctgcccg
       61 atgtcgacct ccgtaagggc gtacccggcc ggcgaagcgc agacaaactc gacgcccgcg
      121 aggacggctg catacgccag cgatcgcgcc acgttgttgc cttcgcccag gtaggcaagg
      181 cgcagacccc gcagggtgcc gaagcgctcc cgaagcgtga gcaggtcggc gagcacctgg
      241 caagggtgtt cgtgatcgct gagcgcgttg atcaccggga ccgacgacca ggacgcaaac
      301 tcatcgaggg gcgcctggtc aaacgtgcgg atcgcgacgc cattcaccat gcgcgacaac
      361 acgcgcgcca catcccgcac gggctctcgg ctcccaatct gcacctcttc tttgccgatg
      421 tagagcgaac gcccaccaag gcggctcatc gcgacctcga acgagacgcg cgtacgcaga
      481 gaaggcttct cgaacagcag cgccagcacc tgtcccgaga gcgcgggacc tgtcccgtcg
      541 cgcttgatgg acagcgcgac atcgagaacc cgttcgaggt cctcggggga gaggtcgagg
      601 atcgacgtca ggtgacggct ttgcacgtgt tccctcggac agtggaatga gacggtcagt
      661 atacgtaatc cactcgcccc acgacactgc cgcctctgca aacacaggtg tgtttgcgat
      721 agtctccgcg cggtcgggga ggctcgcagg ggcgctatgg caaaccatcg atggctcaac
      781 gcggccctcg ccgcctccgc tctgctcgcc ttcctcgtac tcacgttccc caacgactca
      841 gacgacctcg gcatcgaggt cgaatcgcgc gacccgtcgc ccggcattga cgaaatccgg
      901 gtcgccgtca ccggcgcggt agcccgtccg ggcgtggtca cggtacgacc gggtgaccgg
      961 gtcgctgacg cgatcgcact cgcgggcggg ctcaccgcgg acggcgacat gaacgccgtc
     1021 aacctcgcac ggcgcgtccg cgacgaggac caggtagtcg tcccacgcgt cggtgagcgc
     1081 ttcgccctcc tcgatctgaa ccgcgcgacg gcacaggaac tcgaggcgct accgggggtg
     1141 ggcccgacct atgcgaacgc gattgtccag gcgcggacga cccacggccc cttcgagtcg
     1201 accgaccaac tcgtcgagct tggcctcgtc ccggaacgcg tgtacgagcg gtttcgcgac
     1261 ctcgtgacgg tgcactgatg cccgcccttc cagtcgtcac gggcgcattt gtcgccggcg
     1321 cgctgctcgg ggcgctgctc ggcggtcccg tcgcatggac gatgctcctg gtcgcgacca
     1381 ccgtcatcgc ggtgacgctc agatcgaggg agcacttgtc gtggctcgtg ctgatcgccg
     1441 ccgttgcctt cgcgggggtc ggccacctgc gagccctgtc tgcagcggaa tcgcagcaac
     1501 ggtccgaggc gatcgagggc acgcacgtcc tctccggcgt cgtcgaatcg ccgccggacg
     1561 ttcgcggaac cacgcgcttc ttcagcctcc tcgtcgacga ggtggacggt gctcctcggc
     1621 atatccgagt tcgcgtgatc gtcccggcca ccggccaggt gcggctcggt gaccgggtcg
     1681 agctgagcgg ggagctggag tctccgcgcg atggggcgag tcccggtgcc cggcagcgcg
     1741 gaaacgacct cggagtcgag gccgaggtcg ccttccccag tcgatggcgc gtcattggac
     1801 atgagccgcg atcgaggatc gactccgtgc tcggcgcact ccatgagcgg agcatcgcga
     1861 acatcgagcg agtcctgcgt gaaccggagt catcgctcgc ggtcggcatg ttgcttggcg
     1921 tccagcaggc gatgcccgag cggctcacac gcgacctgcg cgcca
//
LOCUS       DAORPG010000400         1960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7388_length_1960_cov_3.286614, whole genome shotgun sequence.
ACCESSION   DAORPG010000400 DAORPG010000000
VERSION     DAORPG010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7388_length_1960_cov_3.286614"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="PLX85_08440"
     CDS             complement(<1..780)
                     /locus_tag="PLX85_08440"
                     /inference="COORDINATES: protein motif:HMM:NF039499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HRC63231.1"
                     /translation="MTLIGLPAQGDWPIPQLFDRRRLELAQSNAPFQEAGLGFSTEFG
                     ELTALLAPVRSLDLVTPVEVRDAAILRALTVRIVKLTRALIASTMAGQGELQALFDRI
                     LFETVVDMGYLLRGSGDRYEAFARHELRHDRELLDHLGRNRALRNGDELPMEQRTRER
                     IEAKLRLAGVHAPDVPLPGEPGDWPSFEERLGSIGEPDARAMHQLGAGSTHGLWHEIA
                     TYHLEGDDLEAKLSWSRPLTQPLFALPIQGGRIMAAYATHLG"
     gene            911..1705
                     /locus_tag="PLX85_08445"
     CDS             911..1705
                     /locus_tag="PLX85_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019927233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRC63232.1"
                     /translation="MAYEHIIYEVEGHTAVITLNRPERLNSISRQMMREINEAYRAAE
                     TDDEVWTILVTATGRAFCTGADVQDQMLTASEERYLGKASDWDAPQEITAPYLQMTKP
                     FICAVNGICCGAGLDMVTTSDIVIASEDATFFDPHVTIGIVSGREMVRLARVLPLNIT
                     MRLALMGRHERMSAQRAYELGLVSELVPPDRLPERAREIASIVNSNAPLAVRGTRLAI
                     RKGLSLPVYEAELLSESYRMRVAVTEDAKEGPRAFAEKRAPDWKVR"
BASE COUNT          312 a          654 c          715 g          279 t
ORIGIN      
        1 gccgaggtgg gtggcgtagg ccgccatgat gcggccgccc tggatcggca gcgcgaacag
       61 cggctgcgtc agcggccggc tccagctcaa cttcgcctcg aggtcgtccc cttcgaggtg
      121 gtaggtcgcg atctcgtgcc acagaccgtg ggtcgagccc gcgccgagct ggtgcatcgc
      181 ccgcgcgtcg ggctcgccga tcgagccgag tcgctcctcg aaggacggcc agtcgccggg
      241 ctcacccggc agcgggacgt caggggcgtg cactcccgcg aggcggagct tcgcctcgat
      301 gcgctcgcgg gtgcgctgct ccatcggcag ctcgtcgccg ttgcgcagcg cacggttgcg
      361 gccgaggtgg tcgaggagct cgcggtcgtg gcgcaactcg tggcgagcga acgcttcgta
      421 gcggtcgccg ctgccgcgca gcagatagcc catgtccaca acggtctcga agaggatgcg
      481 gtcgaacagc gcctgcagct cgccctgccc ggccatcgtc gaagcgatca acgcccgcgt
      541 gagcttcacg atacgtaccg tgagcgcccg caagatcgcg gcgtcgcgca cctcgacggg
      601 ggtgacgagg tcgagcgatc gcacgggcgc gagcagcgcg gtgagctcgc cgaattcggt
      661 cgagaagccg agcccggcct cctgaaacgg cgcgttcgac tgcgccagct cgaggcggcg
      721 gcggtcgaac aactgcggga tgggccagtc gccctgggcg ggcagtccga tgagcgtcat
      781 ctgtcatgta tagcagcggc gtcaccgaac ggcgaaggcg tattgcgagc gtgggtggcg
      841 cgactcgcta tgcgcctgtg atagggtggc cgccgaacga gcatgccggg gagcgaaggg
      901 ggcgccgagg atggcctacg agcacatcat ctacgaagtc gagggccaca cggcggtcat
      961 cacgctgaac cgcccggagc gcctgaactc gatcagccgc cagatgatgc gggagatcaa
     1021 cgaggcgtac cgggccgccg agacggacga cgaggtgtgg accatcctcg tgaccgcgac
     1081 cggccgcgcc ttctgcaccg gcgcggacgt gcaggaccag atgctcacgg cctcggaaga
     1141 gcgctacctc ggcaaggcgt ccgactggga cgcgccgcag gagatcacgg cgccctatct
     1201 ccaaatgacg aagccgttca tctgcgcggt caacggcatc tgttgcggcg ccggcctcga
     1261 catggtcacg acctcggaca tcgtgatcgc gtccgaggac gcgaccttct tcgacccgca
     1321 cgtgacgatc ggcatcgtgt cggggcgtga gatggtgcgg cttgcgcgtg tgctgccgct
     1381 gaacatcacg atgcgcctcg ccctcatggg ccggcacgag cggatgagcg cgcagcgcgc
     1441 ctacgagctc ggcctcgtaa gcgagctggt cccgcccgac cgcctgcccg agcgcgcgcg
     1501 ggagatcgcc tcgatcgtga acagcaacgc gccactcgcg gtgcgcggca cccgcctcgc
     1561 gatccgcaag ggcctctcgc ttcccgtcta cgaggcggaa ctgctctccg agagctaccg
     1621 catgcgggtg gccgtcaccg aagacgccaa ggaaggccca cgcgccttcg ccgagaagcg
     1681 cgcccccgac tggaaggtgc ggtagagcgc agcagcgccg gtcaggccgg ccgacccgcc
     1741 tgctcgcgcc gttcgcgcat catcacgagt gcggtagtat cggcctgtgc accacgaacg
     1801 tcgtggtgat tacgagcgga gggataccga cgtggatcaa ctcgggatgg gggatgtcct
     1861 cgcggggtcg cggttcggca ggcgcgcggc gctgcggggc ggaacgctgg cccttggagg
     1921 gctggcggcg gcggcgctga tcggctgcgg cggggacgac
//
LOCUS       DAORPG010000401         1959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7400_length_1959_cov_3.262605, whole genome shotgun sequence.
ACCESSION   DAORPG010000401 DAORPG010000000
VERSION     DAORPG010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7400_length_1959_cov_3.262605"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PLX85_08450"
     CDS             complement(<1..543)
                     /locus_tag="PLX85_08450"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRC63233.1"
                     /translation="MPPAEDRDALRVLMAPDLPSGLVEHIDRVVSVAEVLARIHGLDE
                     RRVLLAAQGHDLLRGLSKPDLLAAAATRSLEVTDFERNEPVLLHGPLGAIELEARGWM
                     DDPELLEAIRWHTTGHPDYTPIAWALFVADKVEPAKLERWPALDAVAEEMEHSLERAA
                     LRYLDLLHDRLAGEGKPSHPL"
     gene            complement(652..1476)
                     /locus_tag="PLX85_08455"
     CDS             complement(652..1476)
                     /locus_tag="PLX85_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HRC63234.1"
                     /translation="MNNLKTVVLMAAISGLLIAVGGAIGGLGGAIAFAVIAGAMNFAS
                     YWFSADIVLRSSGAHEIQREQDPHLFDMIGEVAQRANMPMPRVYIIDSAQPNAFATGR
                     NPKHAAVAVTAGIRQLLTDRELVGVLGHEISHVKNRDILTSSIVATIASAISMISWMA
                     MWFGGGNRENRNPWTGLVVMLVAPVAATLIQLGISRAREYQADADGARTVGDPEALAS
                     ALAKLQMGAQRIPMDVPASTAHLYIVNPLRGGGVAGLFSTHPPLEKRIERLRSMSV"
     gene            complement(1583..1765)
                     /locus_tag="PLX85_08460"
     CDS             complement(1583..1765)
                     /locus_tag="PLX85_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC63235.1"
                     /translation="MGDTGPEATSITWQAPGATPASGTGATFHPVYTVAGDHVVTVTV
                     CAGAACSTRTVTVHVL"
     gene            1764..>1959
                     /locus_tag="PLX85_08465"
     CDS             1764..>1959
                     /locus_tag="PLX85_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63236.1"
                     /translation="MTSWHVEATLRSTEVQVTLRMLGVAMGVGVGVGAVGVGEGATVG
                     VALAVGVAAGVSVACGVGVAV"
BASE COUNT          263 a          561 c          830 g          305 t
ORIGIN      
        1 gagggggtgc gagggcttgc cctcgcccgc caggcggtcg tggaggaggt cgaggtagcg
       61 gagggcggcg cgctcgaggg agtgctccat ctcctcggcg acggcgtcga gggcgggcca
      121 gcgctcgagc ttggcgggct cgaccttgtc ggcgacgaac agggcccacg cgatgggggt
      181 gtagtcgggg tggccggtgg tgtgccagcg gatggcttcg aggagctcgg ggtcgtccat
      241 ccagccgcgc gcctcgagct cgatcgcgcc gagcgggccg tggagcagca cgggctcgtt
      301 gcgctcgaag tcggttacct cgagggagcg ggtggcggca gcggccagca ggtcgggctt
      361 cgagaggccg cggaggaggt cgtggccctg ggcggcgagg aggacgcggc gctcatccag
      421 gccgtggatg cgcgcgagga cttcggcgac cgagacgacg cggtcgatgt gctcgacgag
      481 gccggagggg aggtcgggcg ccatcagcac gcgcagggcg tcgcggtcct cggcgggggg
      541 catggcggac tcctcgtctg cttcgatccg cagtgtacgc agcggcggat gtcctgcgac
      601 gacggagccc ctcgtgcgag gggctccgtg gggtgcgcgt gggtgtagtg gttagacgga
      661 catcgaacgg aggcgctcga tgcgcttctc caagggaggg tgggtgctga agaggccggc
      721 gacgccgccg cctcgcagcg ggttgacgat atagaggtgc gccgtggagg caggcacgtc
      781 catcgggatg cgctgcgcgc ccatctggag cttggcgagg gcggaggcga gggcctcggg
      841 gtcgccgacc gtgcgagcgc catcggcgtc ggcctggtac tcgcgggcgc ggctgatgcc
      901 gagctggatg agcgtggcgg cgacgggggc cacgagcatg acgacgaggc cggtccacgg
      961 gttgcggttc tcgcggttgc cgccgccgaa ccacatcgcc atccacgaga tcatcgagat
     1021 cgcggaggcg atggtcgcca cgatcgagga tgtgaggatg tcgcggttct tgacgtgcga
     1081 gatctcgtgg ccgaggacgc cgacgagctc gcggtcggta agcagttggc ggatgccggc
     1141 ggtgacggcg acggccgcgt gcttcgggtt gcggccggtg gcgaaggcgt tcggctgggc
     1201 cgagtcgatg atgtagacgc gcggcatggg catgttcgcg cgctgggcga cctcgccaat
     1261 catgtcgaag agatgcgggt cctgctcgcg ttgaatctcg tgcgcaccgg agctgcgcag
     1321 gacgatgtcg gcggagaacc agtaggaggc gaagttcatg gcgccggcga tgacggcgaa
     1381 ggcgatggca ccgccgaggc cgccgatggc gcctccgacc gcgatcagca agccactgat
     1441 ggcggccatc aggaccacgg tcttgaggtt gttcatgtgt ctcaaacact ccctctgttg
     1501 ggtgcgggtg cgtagatcga ttgtatcgcg tcccaccggg cgtcaatgac ggggctttcg
     1561 ggggcggccg cgatggtccc gactagagca cgtggacggt gacggtgcgg gtcgagcacg
     1621 ccgcgcctgc gcagacggtg accgtgacga cgtggtcgcc ggccacggtg tagacggggt
     1681 ggaaggtcgc gcccgtgccg ctcgcggggg tggcgcccgg ggcctgccag gtgatgctgg
     1741 tcgcctcggg gcccgtgtca cccatgacga gctggcacgt cgaggcgacg ttgcggtcga
     1801 ccgaggtgca ggtgacgttg cggatgctcg gcgtcgcgat gggcgtcggg gtcggtgtcg
     1861 gagcggtggg tgtcggcgag ggcgcgaccg tgggcgtcgc gctcgccgtg ggcgtggcgg
     1921 ccggagtgtc ggtcgcgtgc ggtgtcggcg tcgccgtgg
//
LOCUS       DAORPG010000402         1958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7411_length_1958_cov_2.756700, whole genome shotgun sequence.
ACCESSION   DAORPG010000402 DAORPG010000000
VERSION     DAORPG010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7411_length_1958_cov_2.756700"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLX85_08470"
     CDS             <1..474
                     /locus_tag="PLX85_08470"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63237.1"
                     /translation="DALVIAVETSSMAGGRAEQGAGPIIRVGDALHVFSPAMTQWMTS
                     TAQELAAFGDGFRYQRKLMDGGVTEATAYDLYGYETGAACIALGNYHNAGARSKVAPE
                     TVDLGDLEGLVRLFVRMFETVPAFERALPSLKRRYDRLAREAGPLLRRSRGPATS"
     gene            complement(478..1128)
                     /locus_tag="PLX85_08475"
     CDS             complement(478..1128)
                     /locus_tag="PLX85_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63238.1"
                     /translation="MPDRMIAAALPLATIAEGAGVSTDELTEPQAMHDAVLGPLVRVV
                     EEAAGDDVWALVGAASCRLQGADTHSPNLEFITTDAALTTLAEMLDMPSTWGRGAHLA
                     ARRLHFMRGHVPVFVFANPVFHGRYESLSPMDIPSLWDARARIEVERATVLATPLEWE
                     LLLAVVLDSPARLSALRDRMRERGFDGRLLTRLLREGHVEAATEEAVWAVLENDVD"
     gene            1208..1870
                     /locus_tag="PLX85_08480"
     CDS             1208..1870
                     /locus_tag="PLX85_08480"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRC63239.1"
                     /translation="MPKQVTNVASDDALTTIYRLSHDEHEEVIAARVADRLGIKPASI
                     AGMLSRLRRDRLVSIDERKRIALTKAGFDRAEQMVRRHRLAECLLVETLGLEWWRAYE
                     EAHLMEHSISDVTEPLIVEKLGDPTLSPFGYPIPGSQHRHPLSPIRLSDLPAGARTTV
                     ERVYEEDEELLRFFDLEGIRPGAAIEVVETAPYRGTVTVRIEGRDLVLGLQAARRIWV
                     PN"
BASE COUNT          303 a          595 c          763 g          297 t
ORIGIN      
        1 gacgcgctgg tgatcgcggt cgagacctcc tcgatggccg ggggccgggc ggagcagggg
       61 gccgggccga tcattcgggt cggcgacgcg cttcatgtgt tctcgccagc gatgacgcag
      121 tggatgacct cgacggcgca ggagctggcg gcgttcggtg atgggttccg gtaccagagg
      181 aagctgatgg atggcggggt aaccgaggca acggcgtacg acctgtacgg atacgagacg
      241 ggggcggcct gcatcgctct ggggaactat cacaacgcgg gggcccgctc caaggtcgcg
      301 ccggagaccg tcgaccttgg tgacctcgaa gggctggtgc ggctgttcgt gcgcatgttc
      361 gagaccgtac ctgcgttcga gcgggccctg ccctcgctca aacggcggta cgaccggctg
      421 gcgcgggagg cggggccgct gctgaggcgg tcgcgcggcc cggcgacttc gtagccctca
      481 gtcgacgtcg ttttccagga cggcccagac cgcttcctcg gtggcggctt cgacgtggcc
      541 ttcgcggagg aggcgggtga gcaggcgccc gtcgaagccg cgctcgcgca tgcggtcacg
      601 cagggcggag aggcgcgcgg gggagtcgag gacgacggcc agcagcagtt cccattcgag
      661 gggtgtcgcg aggaccgtcg cgcgctcaac ctcgatgcgc gcccgggcat cccagagcga
      721 ggggatgtcc atcggcgaga gcgactcgta acggccgtgg aatacggggt tcgcgaagac
      781 gaacacgggg acgtggccac gcatgaaatg caggcggcgg gccgcgagat gcgcgccgcg
      841 accccaggtc gagggcatgt cgagcatttc ggcgagggtc gtgagggcgg cgtcggtcgt
      901 gatgaactcg agattggggg agtgggtatc agcgccctgg aggcggcagg aggcggcgcc
      961 cacgagggcc cacacgtcgt cgcccgcagc ctcctcgacg acccgcacga gggggccgag
     1021 gacggcgtcg tgcatcgcct ggggttcggt cagctcgtcc gtgcttacgc ctgcaccctc
     1081 tgcaattgtc gcgagcggaa gcgccgccgc gatcatacgg tcgggcatgg atacgcgctc
     1141 ctcggcggtc cgggcagcgc ttgcggctgc gggcggcggg atgtgaggat cgtgccggag
     1201 gacacaggtg ccgaaacagg tcacaaacgt tgcctcggat gacgcgctga cgacgatcta
     1261 ccggctgtcg cacgacgagc acgaggaggt gatcgcggcg cgggtggcgg accgcctcgg
     1321 gatcaagccg gcctcgatcg cgggcatgct gtcacgcctg cggcgcgacc gcctcgtgag
     1381 catcgacgag cggaagcgga tcgcgctgac gaaggcgggg tttgatcggg cggagcagat
     1441 ggtgcggcgg caccggctgg ctgagtgcct gctcgtagag acgctgggcc tggagtggtg
     1501 gcgcgcctac gaggaggcgc acctgatgga gcactcgatc tcggatgtga cggagccgct
     1561 gatcgtcgag aagctgggcg acccgaccct gtcgccattc ggatacccga tccctggctc
     1621 gcagcaccgc catccgctct cccccattcg gctctcggac ctgccggccg gcgcgcggac
     1681 gaccgtggag cgcgtgtacg aggaggacga ggagctgctg cggttcttcg acctcgaggg
     1741 gatccggccg ggcgcggcga tcgaggtggt ggagacggcg ccctatcggg gaacggtgac
     1801 ggtgcgcatc gaggggcggg atctggtgct tggcctgcag gcagcacggc ggatctgggt
     1861 gccgaactag cgtccgtcct cgacgggcgg cacgttgcct cgccgatact cctcgaatct
     1921 ctcgcatgtg cccagacgta cgagatgttt cacgtgaa
//
LOCUS       DAORPG010000403         1952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7451_length_1952_cov_3.609383, whole genome shotgun sequence.
ACCESSION   DAORPG010000403 DAORPG010000000
VERSION     DAORPG010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7451_length_1952_cov_3.609383"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="PLX85_08485"
     CDS             complement(<1..903)
                     /locus_tag="PLX85_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRC63240.1"
                     /translation="MRFYEHEAKQVLQKHGVPLPKRSVATTPEEAETAAAEIGGPVVL
                     KSQVLSGGRMKAGGVKFADTPAEAKDAAAAILEIEISGQKPMGVLVEERRAIAQEYYA
                     AVTWDARAKKPVIIFSDMGGIDIEEVAEQHPDHLSRTNFSNLRPFSDYIAKVAVSKTG
                     VTGNHLGPLTRIVTALARVFVQYDLTLAEINPLARLEDGTFIALDSHMDMEEEARTSQ
                     RALLRELGIPDEDTRLARPPTPFEVAGAQVDAADTRGVAGRVVEFDGNMGLVIGAGGG
                     SLTLFDAVRAAGGKPANYCEIGGNP"
     gene            complement(968..>1952)
                     /locus_tag="PLX85_08490"
     CDS             complement(968..>1952)
                     /locus_tag="PLX85_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRC63241.1"
                     /translation="PVKPSRMIQFMPPQNERMRARVAETAAQVDVLLLNLEDAIPANE
                     KLAAREGLIEVAKSGNAGDTPLWTRINSLDSPWFLDDITEIVAEAGNRIEVIMLPKVQ
                     GPWDITFVDQLLALLEAKHGLQRPIMLHAILETALGVSNVEEIAAASPRMQGISFGPA
                     DLAASRRMKTTRVGGGHPAYVVRGDPVADDPDAPRLTAQQDPWHYTIARMVDACTAVG
                     ILPYYGPFGDISDPVGCEDQFRAAFLLGCVGAWSLHPSQVPIAKKVFSPPPDEVAMAR
                     RIIEALGEGSGVVMLDGKMQDDATYKQAKVMVEVAQLIAARDPEMKAAYGF"
BASE COUNT          258 a          709 c          655 g          330 t
ORIGIN      
        1 ggggttgccg ccgatctcgc agtagttcgc cggcttgcct ccggccgcgc gcaccgcatc
       61 gaagagcgtg agcgagccgc cgccggcccc gatcacgagg cccatattgc cgtcgaactc
      121 gacgacgcgg ccggccacgc cgcgcgtgtc ggccgcgtcc acctgcgcgc ccgcgacctc
      181 gaagggcgtg ggcgggcggg cgaggcgcgt gtcctcgtcg gggatgccga gctcgcgcag
      241 cagcgcccgt tgcgaggtgc gcgcctcctc ctccatgtcc atgtgggagt cgagggcgat
      301 gaacgtgccg tcctcgagac gcgccagcgg gttgatctcc gccagcgtca ggtcgtactg
      361 gacgaacacc cgcgcgagcg ccgtcacgat gcgcgtcagc ggcccgaggt ggttccccgt
      421 cacgccggtc ttcgagaccg ccaccttcgc gatgtagtcc gagaagggcc gcaggttcga
      481 gaagttcgtc ctcgagaggt ggtccgggtg ctgctcggcg acctcctcga tgtcgatgcc
      541 gcccatgtca ctgaagatga tgaccggctt cttcgcacgc gcgtcccagg tgacggcggc
      601 gtagtactcc tgcgcgatcg cgcggcgctc ctcgacgagc acccccatcg gcttctggcc
      661 gctgatctcg atctcgagga tcgcggctgc ggcgtccttc gcctcggcgg gcgtgtccgc
      721 gaacttcacg ccgccggcct tcatccgccc gcccgagagg acctgggact tcaggacgac
      781 cggcccgccg atctcggcgg cggcagtctc ggcctcctcg ggcgtcgttg cgacgctgcg
      841 cttcggcagc ggcacgccgt gcttctgcag cacctgcttc gcttcatgct cgtagaaccg
      901 catgtccgtc ctcgatgtct acttcgggtt gggctgccgc ccgcgcacgt gcttcgggcg
      961 gccgttgcta gaagccgtag gccgccttca tctcggggtc gcgcgcggcg atgagctgcg
     1021 cgacctcgac catcaccttg gcctgcttgt aggtcgcgtc atcctgcatc ttgccgtcga
     1081 gcatcacgac gcccgagccc tcgcccagcg cctcgatgat ccggcgcgcc atcgccacct
     1141 cgtccggtgg cgggctgaac accttcttcg cgatggggac ctggctcggg tgcagcgacc
     1201 acgcgcccac acagccgagg aggaacgccg cccggaactg gtcctcgcag ccgaccgggt
     1261 ccgagatgtc gccaaacggc ccgtagtagg gcaggatgcc gaccgccgtg caggcgtcca
     1321 ccatcctcgc gatcgtgtag tgccaagggt cctgctgcgc cgtcaatcga ggcgcatcgg
     1381 ggtcgtccgc gaccgggtca ccgcgcacca cgtacgccgg atgcccgccg cccacccgcg
     1441 tcgtcttcat ccgccgcgac gccgcgaggt cggccggccc gaacgagatg ccctgcatcc
     1501 gagggctcgc ggccgcgatc tcctcgacgt tggagacacc gagcgccgtc tcgaggatgg
     1561 cgtgcagcat gatcgggcgc tgcagcccgt gcttcgcctc gaggagcgcc agcagctggt
     1621 cgacgaaggt gatgtcccac ggcccctgca ccttgggcag catgatcacc tcgatgcggt
     1681 tccccgcctc ggcgacgatc tccgtgatgt catcgaggaa ccaggggctg tcgaggctgt
     1741 tgatgcgcgt ccacagcggc gtgtcgccgg cgttcccgct cttcgcgacc tcgatcaggc
     1801 cctcgcgcgc cgccagcttt tcgttcgcgg gaatcgcgtc ctcgaggttc agcagcagca
     1861 cgtcgacctg cgcggcggtc tcggcgacac gcgcgcgcat gcgctcgttc tgcggcggca
     1921 tgaactggat catgcggctc ggcttgacgg gg
//
LOCUS       DAORPG010000404         1950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7462_length_1950_cov_3.679156, whole genome shotgun sequence.
ACCESSION   DAORPG010000404 DAORPG010000000
VERSION     DAORPG010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7462_length_1950_cov_3.679156"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PLX85_08495"
     CDS             <1..685
                     /locus_tag="PLX85_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63242.1"
                     /translation="GVAISRPAFAANVLEGQGYRDLFDYTNSFPGDKGELQYEFATQG
                     IPQGAAVQERWYLNGVQQDSLSSSYTWNSGSFAIVSDRLATPNARNIPNGAWTLEVWV
                     AGVLRASSTAFVGVNPQDVTRKPAVETFRFASSASPEQLPGAALTGSSTQVLAFFDYR
                     QAAAVQRVRWVVLRNGQTYYQSAAVPWAGGDHGTWWVGVPVDNARGSWEVQVYFDDII
                     VGTGKLQLS"
     gene            complement(690..1448)
                     /gene="cofE"
                     /locus_tag="PLX85_08500"
     CDS             complement(690..1448)
                     /gene="cofE"
                     /locus_tag="PLX85_08500"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HRC63243.1"
                     /translation="MTPPEVRIIGVRGIPMVQAGDDLAKLIFEAAQAQETPLEAGDVI
                     VVTQRVVSKAEGRVVPLDDFEPTPFAREWAARTGKDARQIEAVLRESVRVVRQARGVL
                     ITETKHGFICANAGVDASNVGGDDLVSLLPEDPDGSCRILRDAIRERTGLEVAVVMTD
                     TFGRPWREGQTNIAIGLAGMRPLHSYEGVADLDGRELRVTMPCPADEIAAAAGLVMEK
                     LAAIPVAIVRGFPYEAGEGSAREIVRPFERDLFP"
     gene            complement(1445..>1950)
                     /gene="npdG"
                     /locus_tag="PLX85_08505"
     CDS             complement(1445..>1950)
                     /gene="npdG"
                     /locus_tag="PLX85_08505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HRC63244.1"
                     /translation="HGAANAEAVADCDVAVIVVPYEAHRTTLESLATPLTGKVVVDAV
                     VPVRFERGPRPVEVPEGSATEQAAAILPDSHVIGAFHNLAAEALLDLERPLDADVLVT
                     GGDAEAKALVSVLAEQIEGVRAVDAGPLRFSRFVEGLTVLLIGINGRYKAHSAPRIVG
                     LPERTAS"
BASE COUNT          282 a          712 c          663 g          293 t
ORIGIN      
        1 cggcgttgcc atctcgcggc ccgcgttcgc ggcgaacgtg ctcgagggcc aggggtatcg
       61 cgacctcttc gactacacga actccttccc cggcgacaag ggtgaactgc agtacgagtt
      121 cgccacgcag ggcatcccgc agggcgcggc ggtgcaggag cgctggtacc tgaacggcgt
      181 gcagcaggac agcctctcct cctcgtacac ctggaactcg ggcagcttcg ccatcgtctc
      241 cgaccggctc gccacgccga acgcgcgcaa catcccgaat ggcgcctgga ccctcgaggt
      301 gtgggtggcg ggggtcctgc gcgcaagctc gaccgcgttc gtcggcgtga acccgcagga
      361 cgtcacgagg aagcccgccg tcgagacgtt ccgcttcgcc tcgtcggctt cgccggagca
      421 actgccgggg gcggcgctca ccggcagctc gacgcaggtg ctcgccttct tcgactaccg
      481 tcaggcggcc gcggtccagc gcgtgcgctg ggtggtgctc cggaacggac agacctacta
      541 ccagtcggct gccgtgccct gggccggcgg cgaccacggc acctggtggg tcggcgtccc
      601 ggtcgacaac gcccggggct cgtgggaggt tcaggtctac ttcgacgaca tcatcgtcgg
      661 gaccggcaag ctgcagctgt cctagcgtcc tacggaaaca ggtcccgctc gaacgggcgc
      721 acgatctcgc gcgccgagcc ctcgcctgcc tcgtacggga acccgcgcac gatggccacg
      781 gggatcgcgg cgagcttctc catcaccagg cccgccgctg ccgcgatctc atccgcgggg
      841 cagggcatcg tgacgcgcag ctcgcgtccg tcgaggtcag cgacgccttc gtaggaatgc
      901 agaggccgca tccccgcgag cccgatggcg atgttcgtct gtccctcgcg ccagggccgg
      961 ccgaacgtgt cggtcatcac caccgccacc tcgaggcccg tgcgctcccg gatggcgtcg
     1021 cgcaggatcc ggcaggagcc gtccgggtcc tccggcagca gcgacacgag gtcgtccccg
     1081 cccacgttgg aggcatcgac gcccgcgttg gcgcagatga agccgtgctt cgtctccgtg
     1141 atcaggacgc ctcgagcctg ccgcaccacg cgcaccgact cccgcagcac cgcctcgatc
     1201 tgccgcgcgt ccttgccggt gcgtgctgcc cactcacggg cgaacggagt gggctcgaag
     1261 tcgtcgaggg gcacgacgcg cccctcagcc ttcgagacga cgcgttgcgt cacgacgatg
     1321 acgtcgcccg cctccagcgg cgtctcctgt gcctgtgcgg cctcgaagat cagcttcgcg
     1381 aggtcgtcgc cagcctgcac catggggatg ccgcgcacgc cgatgatgcg cacctcgggg
     1441 ggcgtcacga ggcggtccgc tcgggcaacc ccacgatgcg cggagcgctg tgtgccttgt
     1501 agcgcccgtt gatgccgatc agcaacaccg tcaggccctc gacgaagcgc gagaagcgca
     1561 gcggccctgc gtcgacggcg cgcactccct cgatctgttc cgcgagcacg ctgacgagcg
     1621 ctttcgcttc ggcatccccg ccggtgacga ggacgtccgc gtcgagcggc cgttcgaggt
     1681 cgagcagtgc ctccgcggcc aggttgtgaa aggcgccgat gacgtgcgag tcggggagga
     1741 tggccgcggc ctgctcggtc gccgacccct cgggcacctc gaccggtcgc ggcccgcgct
     1801 cgaagcgcac cggcacgacc gcatctacca cgaccttgcc ggtgagcggc gttgcgaggg
     1861 actcgagggt ggtccgatgc gcctcgtatg gcacaacgat gaccgcgaca tcgcagtccg
     1921 ccaccgcctc ggcgttggcc gcgccgtgca
//
LOCUS       DAORPG010000405         1950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7463_length_1950_cov_3.543008, whole genome shotgun sequence.
ACCESSION   DAORPG010000405 DAORPG010000000
VERSION     DAORPG010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7463_length_1950_cov_3.543008"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="PLX85_08510"
     CDS             complement(<1..924)
                     /locus_tag="PLX85_08510"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC63245.1"
                     /translation="MAAKRHVIVGGGTAAINAITSIREFDQGASDIVLVAEERPYSRM
                     VLPYYLGKRISRSHVWTLSPDRLAALGVTTQIGRRATALDTAQQRVTLDNGEAVDYDD
                     LLIATGSSAVRAPVPGADLPGVHSFWTLEQADGLIAEVKAGSEVVLVGAGFISFTILN
                     SLVKLGVRLTVIELAPTILPRMIDPTGAAIVTRWLEQHGVTVQTGVRLNGIEEVAGRK
                     RLVLDGGGELSADVVIMATGIRPNLGWLEGAGLEMGRGILVDDHMRTSAPNVYAAGDV
                     AEAFDRVTGQRALHAIEPAAMEHGRIAGANMA"
     gene            complement(914..1402)
                     /locus_tag="PLX85_08515"
     CDS             complement(914..1402)
                     /locus_tag="PLX85_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRC63246.1"
                     /translation="MKVLHIEPEKCTGCLRCEVACSYVQSGEFNPSKSVIRVSAFERG
                     HTSFSPYTCFQCAEGWCMTACPVEAIDISAVGAKEVQDDQCVGCKLCTLACPYGTIFY
                     DPEREKAFKCNLCGGDPQCAHACPTQAITWIEDFTQDWTGAFAAQRSAADLARIGATN
                     GR"
     gene            1651..>1950
                     /locus_tag="PLX85_08520"
     CDS             1651..>1950
                     /locus_tag="PLX85_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRC63247.1"
                     /translation="MKIETGFGETWLGEVPARVRAAEAAGFDSISTPEMRHNSILELT
                     LAAEHTERMELCTGVTIAFPRSPMVMAQSAWDLQQFSKGRINIGLGSQVKGHNIRR"
BASE COUNT          315 a          695 c          635 g          305 t
ORIGIN      
        1 ggccatgttc gcgcccgcga tgcggccgtg ctccatggcg gcgggttcga tggcgtgcag
       61 cgcgcgctgg cccgtcaccc ggtcgaacgc ctcggccacg tcgccggcgg cgtacacgtt
      121 cggggcgctc gtgcgcatgt ggtcgtccac gaggatgccg cgccccatct cgaggccagc
      181 gccctcgagc cagccgaggt tcggacggat gcccgtcgcc atgatcacga cgtcggcgct
      241 caattcgccg cccccgtcga ggacgagccg cttccgtccg gcgacctcct cgatcccgtt
      301 cagtcgcacg ccggtttgga cggtgacgcc gtgctgctcc agccagcgtg taacgatcgc
      361 ggcgccggtg gggtcgatca tccgaggcag gatcgtgggt gcgagctcaa tcacggtgag
      421 ccgcacgccg agcttcacga gcgagttgag gatcgtgaac gagatgaacc ccgctcccac
      481 cagtacgacc tcggagccag ctttcacctc cgcgatgagg ccgtccgcct gctcgagcgt
      541 ccagaacgaa tgcacgcccg gcaggtcggc gcccggcacc ggggcccgca ccgccgatga
      601 gccagtcgcg atcagcaggt cgtcgtagtc gactgcctcg ccgttatcga gcgtgacgcg
      661 ctgctgcgcc gtgtcgaggg ccgtcgcgcg ccgcccgatc tgcgtggtca cccccagtgc
      721 ggcgaggcgg tccggagaca gcgtccatac gtgcgacctc gaaatgcgct tgccgaggta
      781 gtacggcagc accatgcgcg agtaggggcg ctcctcggcg accaggacga tgtcggacgc
      841 gccctggtcg aactcccgga tcgaggtgat cgcgttgatg gcggccgtcc cgccgccgac
      901 gatgacatgt cgcttagcgg ccattcgtcg ccccgatccg cgcgaggtcg gcggccgacc
      961 gttgcgccgc gaaggcgccc gtccagtcct gcgtgaagtc ctcgatccag gtaatcgcct
     1021 gggtcgggca ggcgtgcgca cactgcgggt cgccgccgca caggttgcac ttgaacgcct
     1081 tctcacgttc ggggtcgtag aagatcgtcc cgtacgggca cgcgagggtg cacagtttgc
     1141 accctacgca ctggtcgtcc tgcacttcct tcgctccgac cgccgagatg tcgatcgcct
     1201 cgaccgggca ggcggtcatg caccacccct cggcgcactg gaagcaggta tagggcgaga
     1261 agctcgtgtg tcctcgctcg aacgcgctca cacgaatcac ggacttcgag gggttgaatt
     1321 caccgctctg cacgtaggag caggccacct cgcagcgcag gcagccggtg catttctccg
     1381 gctcgatatg caggactttc accggctcac cctctcatct tgtgtgcagt ccgggcccga
     1441 ctgcgcgcag tctggcaccc cagcccgcgg gcccacaaga ctgcacccgc gtgcaatacg
     1501 gtcagtgcgt gccgttcgcc ggcaaaccct gacgaggcat cggcgcggct cgcccaccgc
     1561 tcgcgaccgc tccaagacca caaccgggcg ctgcggcaca atcccccacg acgacatcca
     1621 atgtgcagcg cctgaaggga ccgcagcagc gtgaagatcg agaccggctt cggggagacc
     1681 tggctcgggg aagtgcccgc gcgcgtgcgc gccgcggaag cggcgggctt cgactcgatc
     1741 agcacgccag agatgcggca caactccatc ctcgagctca ccctcgctgc cgaacacacc
     1801 gagcggatgg agctctgcac gggcgtcacg atcgcattcc cacgctcccc gatggtcatg
     1861 gcgcagtccg cgtgggacct gcagcagttc agcaaaggcc gcatcaacat cggcctcggt
     1921 tcgcaggtca agggccacaa catccggcgc
//
LOCUS       DAORPG010000406         1950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7464_length_1950_cov_3.347230, whole genome shotgun sequence.
ACCESSION   DAORPG010000406 DAORPG010000000
VERSION     DAORPG010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7464_length_1950_cov_3.347230"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1906)
                     /locus_tag="PLX85_08525"
     CDS             complement(<1..1906)
                     /locus_tag="PLX85_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRC63248.1"
                     /translation="MSLLALLAVVLLQWGTGVIILTPALFAVLVVTWYAGLGPGLVSF
                     LLVASAAPVFMALPIGWHYSGAQYAASIAVATPLAVLSAVLLHEGRNSMTVARRSEQR
                     FEAFMGHLPGSAWMKDVHGRYVYANAESLRILKLSREQLYGKTDAQLFPAGLAGQLHD
                     PDALPIAKRDVVAAVESFTQDDGDHDYLIVRFPILDEAGQPEFIGAVGIDITDRARAE
                     RQFAERTAQLQLVIDQVPAFVAYVDRDHRFVTANRPLAQWYSLPASEMRGRRIEDLDP
                     PETLQRTGPYIEQALGGEAVQFEIDAAPAPGVARRWFEARFTPDVAEDGSVAGFSSLI
                     LDITDRKEAESERGLLARLTDLLNRTLNTEETAQALVSALVPGFADGAAIYFAHADAL
                     RGVTRPDTERVASSFADYRVDQEARRGVAEVLRSGLPEFYRESPADLPEPAPVEESGP
                     RERSARHRRASTMLVPLRVHDRVAAVLTAWTDESGRTCQQHDFELFQEVARRAERALE
                     HSLLYEESLHIAEELRVANNAKDEFLGTVSHELRTPITVVLGNASLLATRHRQMDEPT
                     LAASLNDLLGQAERLHRVVENMLILTRLETGTADTEPIAISQSISRIIAEAQRQQSGR
                     VIRLESADWGL"
BASE COUNT          311 a          596 c          766 g          277 t
ORIGIN      
        1 agaggcccca gtcagctgac tcgaggcgga ttacgcggcc ggactgctgc cgctgcgcct
       61 cggcgatgat gcggctgatg ctttgtgaga tggcgatggg ctcggtgtcc gcggtccccg
      121 tctcgagacg ggtcaggatc agcatgttct ccacgacgcg gtggaggcgc tcggcctggc
      181 cgaggaggtc gttgagcgag gccgcgagag tcggctcgtc catctggcga tgacgggtcg
      241 cgagcagcga ggcattgccg aggacgacgg tgatcggcgt gcgcagctcg tgcgagacgg
      301 tgcccaggaa ctcgtccttt gcgttgttgg cgacgcgcag ctcctcggca atgtgcagcg
      361 actcctcgta aaggagggaa tgctccagcg cgcgttcggc gcggcgggcg acctcctgga
      421 acagctcgaa gtcgtgctgc tgacaggtgc ggccggactc gtcggtccag gcggtgagga
      481 ccgcggcgac gcgatcgtgg acgcgcagcg ggacgagcat ggtcgaggcg cgacgatgac
      541 gcgcggaccg ctcgcgcggg ccggactcct cgacgggcgc cggctccggg agatcggcgg
      601 gcgactcccg gtagaactcc gggagtccgc tgcgcagcac ctcagccacg ccacgccggg
      661 cctcctggtc gacgcggtag tccgcgaagc tgctggcgac ccgctcggtg tcgggccgcg
      721 tcacgcctcg cagcgcgtct gcgtgcgcga aatagatggc cgcgccatcc gcgaatccgg
      781 ggacgagggc cgagacgagc gcctgggcgg tctcctcagt attgagggtg cggttgagca
      841 ggtcggtgag gcgggcgagc aggccgcgct cggattcggc ctccttgcgg tcggtgatgt
      901 cgaggatgag cgacgaaaag ccagcgacgc tgccgtcttc ggcgacgtcc ggcgtgaacc
      961 gcgcctcgaa ccagcgccgg gcaacccctg gggcgggcgc ggcatcgatc tcgaactgga
     1021 ccgcttctcc gccgagggcc tgctcgatgt aggggccggt gcgctggagg gtctcgggcg
     1081 ggtcgaggtc ctcgatgcgc cggccgcgca tctcggaggc ggggagcgag taccactgcg
     1141 ccagcgggcg gttggccgtg acgaagcgat ggtcgcgatc gacgtacgcc acgaaggcgg
     1201 ggacctggtc gatcacgagc tggagctgtg ccgtgcgctc agcgaactgg cgctcggcgc
     1261 gggcccggtc cgtgatgtcg atgccgacgg cgccaatgaa ctcaggctgc ccggcctcgt
     1321 cgaggatggg gaagcgcacg atgaggtagt catggtcgcc atcgtcctgg gtaaaggact
     1381 cgaccgcggc gacgacgtcg cgtttcgcga tcgggagggc gtcagggtcg tggagctggc
     1441 cggcgagtcc cgcggggaac agctgagcgt ccgtcttccc gtacagctgc tcgcgggaga
     1501 gcttgagaat ccggagggat tcggcattcg cgtagacgta gcgtccgtgg acgtccttca
     1561 tccaggcgga tccggggaga tgccccatga acgcctcgaa gcgctgttcg ctgcgacggg
     1621 cgacggtcat cgaattgcgg ccctcgtgga ggaggacggc tgagaggacg gcgaggggcg
     1681 tggcgacggc gatcgaggcg gcgtactggg cgcccgagta gtgccagcca atgggaagag
     1741 ccatgaacac cggcgcggcg gatgcgacga gcaggaagga gacgagtccc ggcccaagcc
     1801 cggcgtacca ggtcacgacg agcacggcga agagggccgg cgtgaggatg atcacgccgg
     1861 tgccccactg cagcaggacg accgccagga gcgcgagcag cgacacgacc agggcaaacc
     1921 ggcacgcgcg ggagccgctg agcggcgggg
//
LOCUS       DAORPG010000407         1948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7479_length_1948_cov_3.241416, whole genome shotgun sequence.
ACCESSION   DAORPG010000407 DAORPG010000000
VERSION     DAORPG010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7479_length_1948_cov_3.241416"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="PLX85_08530"
     CDS             complement(<1..1143)
                     /locus_tag="PLX85_08530"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRC63249.1"
                     /translation="MQVDPKRLARALNPQTLVVVGDKGPNFQWLENNKDFEGKLYSVQ
                     VDPNEIAGIEERGYTNFTKLEDVPEDVDLVICAVPRNVAPYIVNDAVKKHVGGMVIFT
                     AGFAETGEELGAQLQERIVEMARAEGLPIVGPNCMGVHNARLGVKFTADQPKVGDGNV
                     SYITQSGTHGASLSMLSPQLGIKVTRTVSIGNAVVLNEADYLEFLRDDPDTHYIGMYL
                     EGVKDGRRFFEVLRETVKKKPVVIWKGGQNEAGQRATRSHTASLATSQALWDGMMRQV
                     GAIGVNTLDEMLDVMACLVHNKPPTGRRVALMAMTGGQSVAISDAFGRASLDVPELSA
                     SSYERLGEFFNIIGGSYRNPFDMAGTIGMAGDPGNFYKILDILAEDP"
     gene            complement(1148..1840)
                     /locus_tag="PLX85_08535"
     CDS             complement(1148..1840)
                     /locus_tag="PLX85_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRC63250.1"
                     /translation="MSDPIATARAAGRTLLTEVESKELLHNAGIRVTQARLATSADEA
                     ASIAAEFGFPVVLKVVSGDIAHKSDVGGVELGLADAGAVRAAYDRIMASAKQAVPTAK
                     IDGVSVQQMATPGTEVIVGLTTDAQFGPVMMFGLGGIMVEVLKDVAFRIVPLEQRDAK
                     QIIREIKGFPVLEGFRGKPGADLSALESMILQVSEFAASHPEVAELDLNPVFAYPDGA
                     IAVDARVVLAAE"
BASE COUNT          295 a          579 c          735 g          339 t
ORIGIN      
        1 cgggtcctcg gcgaggatgt cgaggatctt gtagaagttg ccggggtcgc cggccatgcc
       61 gatggtgccg gccatgtcga acgggttgcg gtaggagccg ccgatgatgt tgaagaactc
      121 gccgaggcgc tcgtaggagg acgcggagag ctcggggaca tcgaggctgg cgcggccgaa
      181 cgcgtccgag atggcgaccg actggccgcc ggtcatcgcc atgagggcga cgcgacgccc
      241 ggtgggcggc ttgttgtgga ccaggcaggc catcacgtcg agcatctcat cgagcgtgtt
      301 cacgccgatg gcgcccacct gacgcatcat gccgtcccag agcgcctgcg aggtcgcgag
      361 cgacgcggtg tgggaacgcg tggcgcgctg acccgcctcg ttctggccac ccttccagat
      421 gacgacgggc ttcttcttca cggtctcgcg cagcacctcg aagaagcgac ggccgtcctt
      481 cacgccctcg aggtacatgc cgatgtagtg cgtgtccggg tcgtcgcgga ggaactcgag
      541 gtagtcggct tcgttgagga cgacggcgtt gccgatggag acggtgcgcg tgaccttgat
      601 gccgagctgc ggcgagagca tcgagaggct cgcgccgtgg gtgccgctct gggtgatgta
      661 ggagacgttg ccatcgccga ccttgggctg gtcggcagtg aacttcacgc cgaggcgggc
      721 gttgtgcacg cccatgcagt tggggccgac gatggggagg ccctcggcgc gggccatctc
      781 gacgatgcgc tcctggagct gtgcgccgag ctcctcaccg gtctcggcga agccggcggt
      841 gaagatgacc atgccgccga cgtgcttctt cacggcgtcg ttcacgatgt agggggcgac
      901 gttgcgcggg accgcgcaga tgacgaggtc gacgtcctcg gggacgtctt cgagcttggt
      961 gaagttggtg tagccgcgct cctcgatgcc ggcgatctca ttgggatcga cctggacgga
     1021 gtagagcttg ccctcgaagt ccttgttgtt ctcgagccac tggaagttgg ggcccttgtc
     1081 gccgacgacg acgagggtct gcgggttgag ggcgcgggcg agacgcttgg ggtctacctg
     1141 catcgtgcta ctccgccgcg aggacgacgc gggcgtccac ggcgatggcg ccgtccgggt
     1201 aggcgaagac ggggttgagg tcgagctcgg cgacctcggg gtgggaggcg gcgaactcgg
     1261 agacctggag gatcatgctc tcgagggcgc tcaggtccgc gccgggcttg cctcggaagc
     1321 cttcgaggac ggggaagccc ttgatctcgc ggatgatttg cttcgcgtcg cgctgctcga
     1381 gcgggacgat gcggaaggca acgtccttga gcacttcaac catgatgccg ccgaggccga
     1441 acatcatgac gggcccgaac tgagcgtccg tggtcaggcc gacgatcacc tcggtgcccg
     1501 gggtggccat ctgctggacg gagacgccgt cgatcttcgc cgtcggcacg gcctgcttcg
     1561 cgctggccat gatgcggtcg taggcggcgc ggaccgcgcc ggcgtcggcg aggccgagct
     1621 cgacgccgcc gacgtctgac ttgtgggcga tgtcgccgga gacgaccttg aggacgacgg
     1681 ggaagccgaa ctcggcggcg atggaggctg cttcgtcggc ggaggtcgcc aggcgggcct
     1741 gcgtgacgcg gatgccggcg ttgtggagca gctctttcga ctcgacctcg gtcagcagcg
     1801 tacggccagc ggcgcgtgcc gtcgcgatgg gatcactcat gtggtctctc tcctatatgt
     1861 aatgaagggc agccgaatca tagggacggc tcccatgcgc cgcaaccgaa gcggggcggt
     1921 tgcgcgggcg aggcgaggcc ttgacgat
//
LOCUS       DAORPG010000408         1946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7493_length_1946_cov_3.775251, whole genome shotgun sequence.
ACCESSION   DAORPG010000408 DAORPG010000000
VERSION     DAORPG010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7493_length_1946_cov_3.775251"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLX85_08540"
     CDS             <1..309
                     /locus_tag="PLX85_08540"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HRC63251.1"
                     /translation="VALALPGDRVITREGTLEGRIALAPRGDSGFGYDPVFELADGRT
                     VAESGELKQLVSHRAVAIRAMMDVLRSLREEWHDRSAAPHDLSDPGLPGAGGRTGHAG
                     "
     gene            230..1300
                     /gene="moaA"
                     /locus_tag="PLX85_08545"
     CDS             230..1300
                     /gene="moaA"
                     /locus_tag="PLX85_08545"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRC63252.1"
                     /translation="MTDQLPPTTSPTPDSPAQAGGPATLVDRLGRPLRDLRISVTDRC
                     NFRCRYCMPREVFGADFAFLPRNEILTFEEITRLARLFAANGVRKVRLTGGEPTLRAE
                     LPKLIEMLAEIPDLDIAMTTNGSLLTQLAKPLADAGLRRVTVSLDSLDDAVFRAMNDV
                     NFPVAKVLEGLEAADAAGLGPIKVNAVVKRGMNDHTIVDLARFIRDRGYILRYIEYMD
                     VGASNGWRLDDVVPGAEVVEMISREMPLEPAEPNYRGEVAKRWRYVDGSGEVGVITSV
                     TQPFCGDCSRVRLSAEGQLYTCLFAFQGTDFREALRSGATDEELQALLRSVWQVRDDR
                     YSELRSENTQGLRRVEMSYIGG"
     gene            1331..1813
                     /gene="moaC"
                     /locus_tag="PLX85_08550"
     CDS             1331..1813
                     /gene="moaC"
                     /locus_tag="PLX85_08550"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020372465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRC63253.1"
                     /translation="MTTGELSHLDAEGHARMVDVSAKADTTREATARGRVSMLASTLA
                     LVVEGRMPKGDVLAVARVAGIMAAKRTPDLIPLCHPLQLTGVEVMLAPDHGSPGIEIE
                     ATVRTTGKTGVEMEALTAVSAAALTVYDMCKAVERSMRIEDIRLVAKSGGKSGDFRAE
                     "
BASE COUNT          299 a          719 c          633 g          295 t
ORIGIN      
        1 gtcgcgctcg ccctgcccgg cgaccgcgtc atcacgcgcg agggcacgct cgagggtcgt
       61 atcgccctgg cgccgcgcgg cgactccggg ttcggctacg acccggtctt cgagctggcc
      121 gacggccgca cggtcgccga gtccggcgaa ttgaagcagc tggtcagcca tcgggccgtc
      181 gcgattcgtg cgatgatgga cgtactacgt tccctccgcg aggaatggca tgaccgatca
      241 gctgcccccc acgacctctc cgaccccgga ctccccggcg caggcgggcg gaccggccac
      301 gctggttgac cgcctcgggc ggcccctgcg tgacctccgc atctccgtca ccgaccgctg
      361 caacttccgc tgtcgctact gcatgccccg cgaggtcttc ggcgctgact tcgcgttcct
      421 gccgcgcaac gagatcctga ccttcgagga gatcacgcgc ctcgcgcgcc tcttcgcggc
      481 caatggcgtg cgcaaggtcc gcctgactgg cggcgagccc acccttcgcg cggagctccc
      541 gaagctgatc gagatgctcg ccgagatccc ggacctcgac atcgcgatga ccaccaacgg
      601 ctcattgctg acgcagctcg cgaagccgct cgcggacgcc ggcctgcggc gagtgaccgt
      661 ctcgctcgac tcactcgatg acgcggtgtt ccgggcgatg aacgacgtga acttcccggt
      721 cgcgaaggtg ctcgagggcc tcgaggcggc cgacgcggcg ggcctcggcc cgatcaaggt
      781 caacgccgtc gtgaagcgcg gcatgaacga ccacacgatc gtggacctcg cccgcttcat
      841 ccgggaccgc ggctacatcc tccgctacat cgagtacatg gacgtcggcg cgagcaacgg
      901 ctggcgcctc gacgacgtcg ttcccggcgc cgaggtggtc gagatgatca gccgggagat
      961 gccgctggag cccgccgagc caaactaccg cggcgaggtc gccaagcgct ggcgctatgt
     1021 cgacggcagc ggcgaggtgg gcgtcatcac ctcggtgacg caaccgttct gcggcgactg
     1081 ctcgcgcgtc cgcctctcgg cggagggcca gctctacacc tgcctgttcg ccttccaggg
     1141 caccgacttc cgcgaggcgc tgcgctccgg ggccaccgac gaggagttgc aggccctcct
     1201 gcgctcggtc tggcaggtgc gtgatgaccg ctactcggag ctgcgctctg agaacacgca
     1261 gggcctccgc cgcgtggaga tgtcctacat cggcggatag ccgcagaggc agcgcggaag
     1321 ggcccttcga gtgacaacgg gcgaactttc tcacctcgac gccgaaggac acgcgcgcat
     1381 ggtcgacgtg tccgcaaagg ccgacaccac gcgtgaggcc accgcccgag gacgcgtctc
     1441 aatgctcgct tcgacgctcg cgctggtcgt cgaagggcgc atgccaaagg gcgacgtgct
     1501 cgccgttgcg cgggtcgcgg gcatcatggc cgcgaagcgc acacccgacc tcatcccgct
     1561 ttgccacccg ctgcagctca ccggcgtcga ggtgatgctc gcgcccgatc acggctcccc
     1621 gggcatcgag atcgaggcaa ccgtccgcac gaccgggaag acgggcgttg agatggaggc
     1681 gctcacggcg gtcagcgccg ccgccctcac cgtctacgac atgtgcaagg ctgtcgaacg
     1741 ctccatgcgt atcgaggaca tccgcctcgt ggcgaagtcc ggtggaaagt ccggcgactt
     1801 ccgcgccgag tagcgcgtcg gcctacatcc ttccctcgcg cgccccaccc gaaccgtcgc
     1861 gccgctgctg cccgttgcgg ctcgaaccgc cccccatgaa tcccgcgacc gcatcctggg
     1921 ccagatcccc cagccgctgc tgcagc
//
LOCUS       DAORPG010000409         1930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7592_length_1930_cov_3.268267, whole genome shotgun sequence.
ACCESSION   DAORPG010000409 DAORPG010000000
VERSION     DAORPG010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7592_length_1930_cov_3.268267"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..533
                     /locus_tag="PLX85_08555"
     CDS             297..533
                     /locus_tag="PLX85_08555"
                     /inference="COORDINATES: protein motif:HMM:NF022829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HRC63254.1"
                     /translation="MQPRATREDFERALAGLEYPVSKAAVVRKAQDHGGIDTEADAIL
                     AQLPDRSFDSFPDLVEAIRATYRSQTDGDVASPV"
     gene            695..>1930
                     /locus_tag="PLX85_08560"
     CDS             695..>1930
                     /locus_tag="PLX85_08560"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRC63255.1"
                     /translation="MSPQPVRRALLVGVNQYADPRNNLKGCVNDVLLMGKTITEHFGF
                     NPEDIRLLTDTRATTANIRERLNWLVDGATPGSVLLFHYSGHGSQVRDRNGDELEDGL
                     DEIICPHDLDWDDPFTDDEFAKAIEGIADGVNFTLVLDCCHSGTGTREFFKEPGARGR
                     DQTRFLVPPPDIAFRMAAGVEVDPGTPERTVNMVGFRELPTRQFGASLIDQNAILISG
                     CRSDQTSADAWIDSDYHGALTYSLVEAIKNKRFSLSYADLVSTAGNWLEVNSYEQVPQ
                     LESRQTMREWSFLDTRPFEGGAAAVGWRPPAESMAAPADARVIFVHGIGNHVAGYSDR
                     WRAAFNRYLSLPLDNFNEVVWDDVFDERSRGLRGLEALPELQPEEQARADAITEEIRD
                     LLESRYDLASDLAEASAAGP"
BASE COUNT          318 a          701 c          577 g          334 t
ORIGIN      
        1 tgggcactct actgtaagga aatgggagtt gtcgatatgg ctacgtaacg cgataaagtt
       61 caattgtaat gaaatcgaaa tgtctccctg ggctgcgcct tcgagcccgt gagcgtcgga
      121 attgatgcat ctgcgaggaa ttcggcgcgc gggacctccg agcgcaagaa ccggcgcgct
      181 acggtgctct cactgaaggg gggcacccat gtacatcggt gtcggtacgg tactcgcggt
      241 catcctcatc atcctcctgc tcgtctggct cttctgagcc tgggagactc ggaccaatgc
      301 agccccgcgc cacccgcgag gactttgagc gggcgctcgc aggcctcgag taccccgtat
      361 cgaaggccgc cgtggtccgc aaagcgcagg accacggcgg catcgatacc gaggctgacg
      421 ccatcctcgc gcagcttcct gaccgttcct tcgattcctt ccctgacctg gtcgaggcga
      481 ttcgcgccac ctaccggtcg cagacagatg gggacgtcgc ttcgcccgtc tgacgttcgt
      541 ggctgcccgg cggccctccg agctctccgt ggcgttggtg tatagtcccg ccgacgcctc
      601 gttgttcctg ccttgcgcgt gattccgtcc gcaggcagcg gccgaggccg cgcctcctcg
      661 cacgcgcgcc cctccgggca ggaaggaccg tcttgtgagc ccgcaacccg tccgtcgagc
      721 gctcctcgtg ggcgtcaacc agtacgcgga ccctcgcaat aacctcaagg gctgcgtcaa
      781 cgatgtcctg ctgatgggca agaccatcac cgagcatttc ggcttcaatc cggaggacat
      841 ccgcctcctc accgacacgc gcgctaccac cgccaacatc cgcgagcgcc tgaactggct
      901 ggtcgatggc gcgacgcccg gctccgtgct gctcttccac tactcgggcc acggttccca
      961 ggttcgcgac cgcaacggcg acgagctcga agacggcctc gatgagatca tctgtcccca
     1021 cgacctcgac tgggatgacc ccttcaccga cgacgagttc gcaaaggcca tcgagggcat
     1081 tgcggacggg gtgaacttca ccctcgtcct cgactgctgc cattccggca cgggcacacg
     1141 tgagttcttc aaggagcccg gcgcccgcgg ccgcgatcag acgcggttcc tcgtcccccc
     1201 gcccgacatc gccttccgca tggctgcggg cgtcgaggtg gaccccggca ccccggaacg
     1261 caccgtcaat atggtcggct tccgtgagct tccgacgcgc cagttcgggg cgagtctcat
     1321 cgaccagaac gccatcctca tctcgggctg ccgctccgat cagaccagcg ccgacgcctg
     1381 gatcgactct gactaccacg gcgcgctgac ctacagcctc gtcgaggcca tcaagaacaa
     1441 gcgcttctcg ctctcctacg ccgacctcgt ctcgaccgcc ggcaactggc tcgaggtcaa
     1501 cagctacgag caggtgccgc agctcgagag ccgccagacg atgcgcgaat ggtcctttct
     1561 cgacactcgc cccttcgagg gtggggccgc ggcggtcggc tggcgcccgc cggccgagtc
     1621 catggccgcc cctgccgacg cgcgcgtcat cttcgtgcac ggcatcggga accacgtcgc
     1681 gggctactcc gaccgctggc gcgccgcctt caaccgttac ctcagcctgc cgctcgacaa
     1741 cttcaacgag gtcgtctggg acgatgtctt cgatgagcgc tcacgtggac tccgcggcct
     1801 cgaggccctc ccggagctcc agccggagga gcaggcccgc gccgacgcca tcaccgagga
     1861 gatccgtgac ctcctcgaaa gccgctacga cctcgccagt gacctcgccg aggcgagcgc
     1921 cgccgggccg
//
LOCUS       DAORPG010000410         1916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7676_length_1916_cov_3.557227, whole genome shotgun sequence.
ACCESSION   DAORPG010000410 DAORPG010000000
VERSION     DAORPG010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7676_length_1916_cov_3.557227"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="PLX85_08565"
     CDS             complement(<1..813)
                     /locus_tag="PLX85_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HRC63256.1"
                     /translation="MNVRRSPRLLLLVPLLALALGLSLACSDDGGDSDLPLTVGALVP
                     VTGSLSSYGEASQAALKLAADSINNGSGAKVNLLIEDTKTDPPTALAKLQSLHERGAK
                     IVIGPFASSEVKAVKDFADQNGIVLVSPLSTARSLAVADDNVFRFTPDDDKEGVAVAA
                     LAWADGIKTLIAVSRNDEGNLGLQTSMKSAFEKLGGKVVSGVTYPADEKEFSDEMRTV
                     AAALTGAKASGGPVGIYLTAFGEVDALFNAANTNADLKAVKWYGSDSVALSAD"
     gene            complement(919..1212)
                     /locus_tag="PLX85_08570"
     CDS             complement(919..1212)
                     /locus_tag="PLX85_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRC63257.1"
                     /translation="MTSYRRRRRIVAKAVGRVQGVGYRAFCAHEATRLLVEGYAKNLP
                     DGRTVEVVAEADEPTLRTFIERLREGPTMSHVDSVTFRWEDSTGEFDGFEAVI"
     gene            complement(1232..>1916)
                     /locus_tag="PLX85_08575"
     CDS             complement(1232..>1916)
                     /locus_tag="PLX85_08575"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HRC63258.1"
                     /translation="IGGKVAVDLPAGKNLVGAFHQPHAVIADTDTLRTLPRRSYTEGF
                     GEVIKHALILDPSLLRDLERHAGSLAAGTPDPEVLASVFTRSARLKALVVSADPTERG
                     LRAILNYGHTIAHGIEAAAGYGNDYLHGEAVAVGMMGAARIAVRLGLLDEEVLARQGD
                     VLRAFGLPLSAPGVPANAILNAMRLDKKVVAGRLRFILLEDVGRPVVRDDVPEDLVAE
                     VVRGLVASS"
BASE COUNT          304 a          606 c          709 g          297 t
ORIGIN      
        1 gtccgcgctc aaggcgacgc tgtcgctgcc ataccacttc accgccttga ggtcggcgtt
       61 cgtgttggcg gcgttgaaga gtgcgtccac ctcgccaaac gcggtgaggt aaatgccgac
      121 ggggccgccc gatgccttcg cgccggtcag cgccgccgcg acggtgcgca tctcgtcgga
      181 gaactccttc tcgtcggcgg ggtacgtaac gccggagacg actttgccgc cgagcttctc
      241 gaaggcgctc ttcatcgagg tctgcagccc gaggttgcct tcgtcgttgc ggctgacggc
      301 gatcagcgtc ttgatgccgt ccgcccaggc gagcgcggcg acggcgacgc cttccttgtc
      361 gtcatccggg gtgaagcgga agacgttgtc gtcagcgacc gcgagggaac gcgcggtgct
      421 caacgggctg acgaggacga tgccgttctg gtccgcgaag tccttgaccg cctttacctc
      481 ggagctggcg aaggggccga tcacgatctt ggcgccgcgc tcatgaagtg actgcagctt
      541 ggccagcgcg gtcggggggt cggtcttggt gtcctcgatg aggaggttca ccttggcgcc
      601 ggagccattg ttgatggagt ccgccgccag cttgagcgcc gcctggctcg cttcaccgta
      661 ggaggacagg gagccggtga ccgggacgag cgcgcccacc gtcagcggga ggtcgctgtc
      721 gccgccatcg tcggagcagg cgagcgagag gcccagcgcg agcgcgagca agggcacgag
      781 gagcagcagc cgtggcgagc ggcggacgtt cattcggatc cccttcgaag cggatgctgc
      841 cgcagcagtg agcgcgagtg tggggagact accatacggg ccctcaacga gccgcccgcc
      901 gcgaggaggg ggctgggctc agatcacggc ctcgaagccg tcgaactcgc ccgtcgagtc
      961 ctcccagcgg aaggtgacgc tgtcgacgtg cgacatcgtg gggccctctc ggaggcgctc
     1021 gatgaaggtg cggagggtcg gttcgtcggc ctcggcgacg acctcgacgg tgcggccgtc
     1081 ggggaggttc tttgcgtagc cctcaacgag gagtcgggtt gcctcatggg cacagaacgc
     1141 gcggtagccg acgccctgca cgcggccaac ggccttcgcc acgatgcgac gacgacgacg
     1201 ataggaggtc acgagcgcgg ctcctcggtc gtcacgagct ggcgacgagg ccccggacga
     1261 cctcggcgac gaggtcttcg gggacgtcgt cgcggacgac ggggcggccg acgtcctcga
     1321 gcaggatgaa gcgcagcctg ccggcgacga ccttcttgtc gagacgcatg gcgttcagga
     1381 tcgcattcgc ggggacgccg ggcgccgaaa gaggcagacc gaaggcgcgc aacacgtcgc
     1441 cctgacgagc gaggacctcc tcgtcgagga gtcccaagcg aacggcgatg cgcgccgcgc
     1501 ccatcatgcc gacggcgaca gcttcaccgt ggaggtagtc attgccgtag ccggcggcgg
     1561 cctcgatgcc gtgggcgatg gtgtggccgt agttcaggat ggcacgcaga ccacgctcgg
     1621 tggggtccgc cgacaccacc agcgccttga ggcgcgccga acgcgtgaag acgctcgcca
     1681 gaacctcggg atccggtgtc ccggcggcga gcgagccagc gtgtcgttcg aggtcacgca
     1741 gcagcgaggg gtcgaggatg agggcgtgct tgatcacctc gccaaagccc tcggtgtagg
     1801 agcgcctcgg caatgtgcgc agggtgtccg tgtcggcgat gacggcgtgc ggctggtgga
     1861 aagcgccgac caggttcttc ccggccggga ggtcgacggc gaccttgccg ccgatg
//
LOCUS       DAORPG010000411         1915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7684_length_1915_cov_2.823118, whole genome shotgun sequence.
ACCESSION   DAORPG010000411 DAORPG010000000
VERSION     DAORPG010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7684_length_1915_cov_2.823118"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /gene="cgtA"
                     /locus_tag="PLX85_08580"
     CDS             <1..859
                     /gene="cgtA"
                     /locus_tag="PLX85_08580"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HRC63259.1"
                     /translation="APRFAQWGSEGDQRALRLELKLLADVGLVGLPNAGKSTLLRAWS
                     AATPKVAPYPFTTLEPELGVVAVGYETFVAADMPGLIEGASQGVGLGHEFLRHIERTR
                     VLVHVLDMTREDPAADRALIDAELDAFGHGLAEKPQVIALNKVDDPEARERATALGVL
                     LDAEGVVWRPISAATREGTQELAQQAWRLLQEVRAQESDVAPLPVLHPEPRRQRFEAS
                     RAEDGVAVITGNTPEWLANTLNLNDFEARQELFDRLRKLGVGRALDRLKVRPGERIRI
                     GDVEVEWGL"
     gene            856..1455
                     /gene="nadD"
                     /locus_tag="PLX85_08585"
     CDS             856..1455
                     /gene="nadD"
                     /locus_tag="PLX85_08585"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HRC63260.1"
                     /translation="MRAGVLGGTFDPPHLAHLAIGAAAKQALHLDRVLFVPAGDPWRK
                     AGREVSPASVRLRMLRAALEPLPWAEISAIEVDRPGPSYSADTMEELARESAPGADWW
                     FIVGDDALTDLPNWHEPERLLAAARLALVWRPPGDPIIPGEVRTRFADIEARLDVVPM
                     PPLDVSSTALRERVRSGRATSILLPEGVRTVVDELGLYR"
     gene            complement(1452..>1915)
                     /locus_tag="PLX85_08590"
     CDS             complement(1452..>1915)
                     /locus_tag="PLX85_08590"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC63261.1"
                     /translation="GVDFSMEALRLARRDARRHGHAVRLACGTATTLPFRSDAFDLIT
                     CGYLMKHLTDEELDAFLAEVRRVLAGGGLALLWEFAPSGSARLDAWNRRVLGPRVHDP
                     LLRTTSTLLERAEAAGFEFTRDAILRPFFFPPIPRASILVGKAPEGWQPTS"
BASE COUNT          275 a          686 c          659 g          295 t
ORIGIN      
        1 ggcgccgcgt tttgcgcagt ggggcagcga aggtgaccag cgtgcgctcc gccttgagtt
       61 gaagctgctc gcggacgtcg ggctggtcgg tctgcccaac gctgggaagt ccacactcct
      121 gcgcgcctgg tcggccgcaa cgcccaaggt cgcgccctac ccgttcacaa ccctcgagcc
      181 cgagctgggg gtcgtcgcgg tgggctacga gacattcgtg gcggcggaca tgcccggcct
      241 catcgaggga gcaagccagg gcgttggcct cggtcacgag ttcctgcgcc acatcgagcg
      301 cacacgtgtg ctcgtccacg tgctcgacat gacccgcgag gaccccgccg ccgaccgcgc
      361 actcatcgac gccgaactgg atgcctttgg ccatggcctc gccgagaagc cgcaggtcat
      421 cgccctcaac aaggtcgacg accctgaggc acgcgagcgt gcgaccgccc tgggtgtcct
      481 tctggacgcg gagggcgtcg tctggcggcc catctcagca gccacgcgtg agggcacgca
      541 ggagcttgct cagcaggcct ggcggctact ccaggaggtg cgtgcccagg agtccgacgt
      601 ggcgcccctt ccggtgctgc atccggagcc tcgccgccag cgtttcgagg cgagccgcgc
      661 tgaggatggc gtcgcggtga tcaccggaaa tacccccgag tggctcgcaa acaccctcaa
      721 cctgaacgac ttcgaggcgc gtcaggagct attcgaccgc ctgcgcaaac tcggcgtcgg
      781 ccgcgccctc gaccgcctga aggtccggcc cggcgaacgc atccgcatcg gtgatgtcga
      841 ggtggaatgg ggcctgtgag ggccggcgtc ctcggcggca cgtttgaccc gccgcatctc
      901 gcccacctcg cgatcggcgc tgcggcgaag caggccctgc atctcgaccg ggtcctgttc
      961 gtgcccgccg gcgacccgtg gcgcaaggcc ggccgggaag tcagcccggc gtccgtccgc
     1021 ctccgcatgc ttcgtgcggc cctcgagccg ctgccgtggg ccgagatcag cgccatcgag
     1081 gtggatcgcc ccggaccgtc ctactcggct gacacgatgg aggagctcgc gcgcgaatcg
     1141 gccccggggg cggactggtg gtttatcgtc ggcgatgacg cgctcaccga cctgccgaac
     1201 tggcacgagc ccgagcgttt gctcgccgcc gcgcgcctcg cgctcgtctg gcggccgccc
     1261 ggcgacccta tcatccctgg cgaggtgcgc acccgcttcg cggatatcga ggcgcgcctc
     1321 gacgtcgtgc cgatgccgcc cttggacgtg tcctcaaccg ccctgcggga gcgcgtacgt
     1381 tcgggccgtg cgacctccat cctccttccg gaaggtgtac gcacggtcgt cgacgaattg
     1441 ggcctgtatc gctagctggt gggttgccag ccctcgggcg ccttcccgac gagaatcgaa
     1501 gcgcgcggga tcggcgggaa gaagaacggc cgcaggatcg cgtcgcgggt gaactcaaag
     1561 cccgcggcct cggcgcgttc gagcagcgtc gaggtcgtgc gcagcaatgg gtcgtgcacg
     1621 cgcggcccga gcacgcgccg gttccaggca tcgagtcgcg cgctgccgct cggcgcgaac
     1681 tcccagagca gtgccagtcc cccgccggcg agcactcgcc gtacctcggc gaggaacgcg
     1741 tcgagctcct cgtccgtgag gtgcttcatc aggtagccgc acgtgatgag gtcgaacgcg
     1801 tcgctccgga acggcagagt cgttgcggtc ccgcacgcca ggcgtaccgc gtggccgtgc
     1861 ctgcgcgcgt cgcgcctcgc caggcgcaat gcctccatcg agaagtcgac tcccg
//
LOCUS       DAORPG010000412         1908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7736_length_1908_cov_3.434970, whole genome shotgun sequence.
ACCESSION   DAORPG010000412 DAORPG010000000
VERSION     DAORPG010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7736_length_1908_cov_3.434970"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PLX85_08595"
     CDS             <1..493
                     /locus_tag="PLX85_08595"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRC63262.1"
                     /translation="VEAHAAPWFADALDRDRALRGALQAALHALETDLGPQRSKWRWG
                     SVHGWRLPHLLEHVPALGRWYSRGPYPFPGDGNTVLQASYGVTAGTGRVGTLPGYRQV
                     IDLADLDRSVFQLSTGNSGIPGHRRYGDCIEEFIAGRYRPLLYSTEAIEANVEHRLRL
                     EPA"
     gene            490..1506
                     /locus_tag="PLX85_08600"
     CDS             490..1506
                     /locus_tag="PLX85_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRC63263.1"
                     /translation="MTEPLARRFERYRPLIVDEMRAVVGEDSTRLSAWLRYHLGWEDA
                     DGKALGDSSGKLLRPSALLLAAELMGGAIEEAVPAAAAVELVHNFSLLHDDVEDASER
                     RHGRPAVWTFAGVPQAINAGDGMYTLARLAMHRLLEAGVEERRTLESMRELDEACLRL
                     VEGQYLDISFEDRNDVTRAEYLAMTAGKTAAMFSAPFAMGALLGGASGDLVTAFREFG
                     YHVGLAFQAMDDILGIWGEPEVTGKPVGDDLRSRKMTYPVIAAMASSAVGAALVEAYA
                     DSASTVEALRTLVEEGDGRTHTEHFMMSAVESALEALHAAALAPEAEAVCADFAHLAT
                     ARVR"
     gene            1593..>1908
                     /gene="pgk"
                     /locus_tag="PLX85_08605"
     CDS             1593..>1908
                     /gene="pgk"
                     /locus_tag="PLX85_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRC63264.1"
                     /translation="MLESVVKKKTIRDIDLKNQRVLLRVDYNVQVENGRIVDDTRLRE
                     SLPTINELRKAGACIVICSHRGRPRGGVDESLRNAPVALHLTELLGVPVRTAQDCIGP
                     DAE"
BASE COUNT          285 a          745 c          581 g          297 t
ORIGIN      
        1 cgtcgaggca cacgccgcgc cctggttcgc ggacgccctc gaccgcgacc gcgccttgcg
       61 tggcgccctc caggccgcgc tccacgccct cgagaccgac ctcgggccgc aacgttcgaa
      121 gtggcgctgg ggatccgtgc acgggtggcg cctcccgcac ctcctcgagc acgtccccgc
      181 tctcggccgc tggtactctc gaggtcccta cccgttcccc ggtgacggca acaccgtcct
      241 tcaagccagc tacggcgtga ccgcgggcac aggccgcgtc ggaacgctcc cgggctatcg
      301 ccaggtcatc gacctcgctg acctcgaccg ctcggtgttc cagctctcca ccggcaattc
      361 cggcatcccc ggccatcgcc gctacggcga ttgcatcgag gagttcattg cgggacgcta
      421 ccgcccgctc ctctactcaa ccgaggccat cgaggcgaac gtggagcacc ggctgcgcct
      481 ggagcccgca tgaccgaacc cctcgcccgg cgcttcgagc gctaccgccc cctcatcgtc
      541 gatgagatgc gcgccgtggt cggcgaggac tcgacccgcc tttccgcctg gctgcgctat
      601 cacctcggct gggaggacgc cgacggcaag gcgctgggtg actcgtccgg caaactcctc
      661 cgcccctcgg ccctgctgct cgcggccgag cttatgggcg gcgccatcga ggaggcggtg
      721 cctgccgccg ccgcggtcga gctggtgcac aacttctcgc tcctccacga cgatgtcgag
      781 gacgccagcg agcgccgcca cggccgtccc gccgtctgga cgttcgccgg cgtcccccag
      841 gccatcaacg ccggcgacgg catgtacacc cttgctcgtc tcgccatgca ccgcctgctc
      901 gaggcgggcg tcgaggagcg ccgcaccctc gagtcgatgc gtgagctcga cgaggcctgc
      961 ctccgtctcg tcgagggtca gtacctcgac atctcattcg aggaccgcaa cgacgtcacc
     1021 cgcgccgaat acctggccat gaccgctggc aagaccgcag ccatgttctc cgcccccttc
     1081 gccatgggcg cactcctcgg cggcgcctcc ggcgacctcg tcaccgcctt ccgcgagttc
     1141 ggctaccacg tcgggttggc cttccaggcc atggacgaca tcctcggcat ctggggcgag
     1201 cccgaggtga ccggcaagcc ggtcggcgac gacctccgtt cgaggaagat gacctatccg
     1261 gtcatcgctg ctatggcctc atccgcggtc ggcgccgccc tcgtcgaggc ctacgcggac
     1321 tctgcctcga ccgtcgaggc gctgcgcacc ctcgtcgagg agggcgacgg ccgcacgcat
     1381 acggagcact tcatgatgag cgccgtcgag tccgccctcg aggcactgca tgcggcggcc
     1441 ctggcccccg aggccgaagc cgtctgcgcc gacttcgccc acctcgctac agctcgcgtc
     1501 cgttaatctc tgctggacac aacgccgaca atctggccac tagccaccga tatgctagcg
     1561 tcgaacgaga cggtcgcggc cacggccgct ggatgctgga gagcgtggtg aagaagaaga
     1621 ccatccgcga catcgacctg aagaatcagc gggtcctctt gcgcgtcgac tacaacgtcc
     1681 aggtcgaaaa cggccgtatc gtcgacgaca cccgccttcg cgagtcactt cccaccatca
     1741 acgagctccg caaggcgggc gcctgcattg tcatctgctc ccaccgaggt cgcccccgcg
     1801 gcggcgtcga cgagtcgctg cgcaacgccc ccgtcgccct gcacctcacc gagctgctcg
     1861 gcgtcccggt gcgtaccgcc caggactgca tcggccccga cgccgagg
//
LOCUS       DAORPG010000413         1899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7805_length_1899_cov_3.486985, whole genome shotgun sequence.
ACCESSION   DAORPG010000413 DAORPG010000000
VERSION     DAORPG010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7805_length_1899_cov_3.486985"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PLX85_08610"
     CDS             <1..351
                     /locus_tag="PLX85_08610"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HRC63265.1"
                     /translation="FGIEHNLGLFFDEFPRGEVALAGRTHALARGGAARRATEATVAL
                     AGHGICIGMTVSQPADIDLRTVATVSQSEAGFEEIPQSLACLFSWPLRLAPGDGFEVD
                     LRLSCTVGAEPSSA"
     gene            348..>1899
                     /gene="treZ"
                     /locus_tag="PLX85_08615"
     CDS             348..>1899
                     /gene="treZ"
                     /locus_tag="PLX85_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HRC63266.1"
                     /translation="MTWQPRLGAWPEDGGVRFRVWAPDAERVEVEIEGTQRGTLPLQR
                     EDGGYHAGLFAGLSAGARYRYRLDGRGPFPDPCSRFQPGGVHGPSEVIDPTFPWSDAD
                     WPGIPLTDLVTYELHLGAFTPQGSFAAAAEALPRLAELGVTAIQLLPVADFAGSRGWG
                     YDGVALFAPARCYGRPEELRGLVDRAHALGLAVILDVVYNHFGPDGAYHAEFSRQYFN
                     SAHHTLWGSAVNLDGPGSEAVRDFFVESARHWLHEYHVDGFRLDSTHALVDGGPRHFL
                     AEYTAAVHDATPRAHQPLVIAEDHRNLSALLLPEERGGFGLDAVLADDFHHELRRILA
                     GDDEGYYADYRGDTTDLATCLAQGWLFVGQHAPYWNEPRGTDPTGLAPPHFVHCLQNH
                     DQVGNRPFGDRLTATTTIEAFRAATAVLLTSADTPMLFMGQEWAASTPFQFFTDHHEE
                     LGRAVTAGRRSEFGAWSAFRDEAMRERIPDPQDPATFERSKLDWSERSRQPHAGALAL
                     HERLLQLRH"
BASE COUNT          271 a          716 c          616 g          296 t
ORIGIN      
        1 ttcggcatcg agcacaacct cggcctcttc ttcgacgagt tccctcgcgg cgaggtcgca
       61 ctcgccggcc gcacccacgc gcttgcgagg ggtggcgccg cacggcgggc gacagaagcg
      121 acggtcgcgc tggcaggcca tggtatctgt ataggcatga ccgtgagcca gccggccgat
      181 atcgatcttc gtaccgtcgc cacggtctct cagtccgaag caggcttcga ggagatcccg
      241 cagtcgctcg cctgtctctt cagctggccc cttcgcctcg cacccgggga cggcttcgag
      301 gtggacctcc gtctgagctg caccgtcggg gcggagccgt ccagcgcatg acctggcagc
      361 cgcgcctcgg agcctggccc gaggatggcg gcgtgcgctt ccgggtctgg gcgccagacg
      421 ccgagcgcgt cgaggtggag atcgagggca cacagcgcgg aacgctgccc ctccaacgcg
      481 aggacggcgg gtatcatgcc ggtctcttcg ccgggctcag cgcgggcgcc cgttaccgct
      541 accggctgga tggacgcggc ccgttcccgg acccctgctc ccgctttcag ccggggggcg
      601 tccacggacc gtccgaggtg atcgacccga ccttcccctg gagcgatgcc gactggcccg
      661 gcatcccgct cactgacctc gtgacctacg agctgcacct cggcgccttc actccgcagg
      721 gcagctttgc cgccgcggcc gaggcattgc cgcgcctggc cgagctgggc gtgacggcga
      781 tccagttgtt gccggtcgcc gacttcgccg gctcccgtgg ctggggctac gacggggtgg
      841 ccctcttcgc cccggcgcgc tgctacggcc gcccggagga gctgcgtggc ctcgtcgacc
      901 gcgcccacgc cctcggcctg gccgtcatcc tcgatgtggt ctacaaccac ttcggtccag
      961 atggcgccta tcacgccgag ttcagtcgcc agtacttcaa ttccgcccac cacacgctgt
     1021 ggggatccgc ggtcaacctc gacggtcccg gcagcgaggc cgtgcgcgac ttcttcgtcg
     1081 agagcgcccg tcattggctg cacgagtacc acgtcgacgg ctttcggttg gactccaccc
     1141 acgccctcgt cgacggagga ccgcggcact tcctcgccga atacaccgcg gccgtccacg
     1201 acgcgacgcc acgcgcccac cagccgctcg tcatcgccga ggaccatcgc aacctgagcg
     1261 cgctgctcct ccccgaggaa cgtggcgggt tcgggctcga cgcggtgctg gcggacgact
     1321 tccatcacga gctgcgccgc atcctcgccg gcgacgacga gggctactac gccgattacc
     1381 ggggcgacac gactgatctc gcaacctgtc tcgcgcaagg ctggctgttc gtcggccagc
     1441 acgcgccgta ctggaacgag ccacgcggca ccgatcccac cggactcgcg ccgccccact
     1501 tcgttcactg cctgcagaac cacgaccagg tgggcaaccg tccgttcggc gaccgcctca
     1561 ctgcgacaac gaccatcgag gcgttccgcg ccgcgacggc ggtgctgctc acctcggcgg
     1621 acacgccgat gctgttcatg ggccaggaat gggccgcgtc cacgcccttc cagttcttca
     1681 ccgatcacca cgaggagctc ggccgggccg tcaccgccgg acggcggtcg gagttcggcg
     1741 cctggagcgc gttccgcgac gaggcgatgc gtgagcggat ccccgacccg caggatcccg
     1801 cgaccttcga gcgaagcaag ctcgactggt cggaacggag ccgtcagccg catgccggcg
     1861 cgctcgccct gcacgagcgg ctgctgcagc tccgccaca
//
LOCUS       DAORPG010000414         1899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7807_length_1899_cov_3.223970, whole genome shotgun sequence.
ACCESSION   DAORPG010000414 DAORPG010000000
VERSION     DAORPG010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7807_length_1899_cov_3.223970"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..622)
                     /locus_tag="PLX85_08620"
     CDS             complement(92..622)
                     /locus_tag="PLX85_08620"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRC63267.1"
                     /translation="MSDAPLPPPATEAGTSSRGHAPRSLRTQFVLFALIGVGNAAVDA
                     AVFTFLVAVLDWRHGIAPLAASVVGFCFGAAHSYVWNSRVTFRFGRSADNATVVGQFL
                     SVAVGGAIVSAIAFSVVRALWPDAHTTLAASKLGAIAVGMVWNFSLLRGWVFSHRRRE
                     RLAAQRDFARRTPDPR"
     gene            complement(747..1811)
                     /locus_tag="PLX85_08625"
     CDS             complement(747..1811)
                     /locus_tag="PLX85_08625"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRC63268.1"
                     /translation="MPAAGGFGLVVWRGGSTDDIATTAASGGCDLSAVWVTNPTGEFV
                     VYIYGAPDVVNQPWLTRFAAGQVPAGTAVLIVCEGPTASALSTSATAQGGAGGPDAAA
                     STIEASAVTAAAPAIAAKTALVLDGASGKTLFDKDAHLALPPASLTKMVTAILAIEQG
                     DLDAWVKTDVDSRQMAGSSLMGLLPGDCFQLRDLVYGLLLPSGNDAALAIARHIAGSD
                     DAFVDQMNAFLTRLGLKDSHFVNPHGLDAAGHVMSAYDLAQVAKYGMSLPVFARVVST
                     PQWTAKGSRTINLPNVNGFLSSYPGADGVKTGFTDAAGRTLVASATKNGRRIFVVLLN
                     DDGRYDDAAKLMDWAFQNTN"
BASE COUNT          316 a          572 c          749 g          262 t
ORIGIN      
        1 agcagcaggc cacagattgc gagggcgatg gaggccccgc cgaggcgggg ccgagcaacg
       61 gtgcgttcgg cccgcgggag ggcggcggat gtcatcgcgg gtcgggggtg cggcgagcga
      121 agtcgcgctg ggcggcgagg cgttcgcggc ggcgatggga gaagacccag ccgcggagaa
      181 gcgagaagtt ccagaccatg ccaacggcga tggcgccaag cttgctcgcg gcgagtgtgg
      241 tgtgggcgtc gggccagagc gcgcggacga cggagaaggc aatcgcggag acgatcgcac
      301 ctccaacggc gaccgagagg aactgaccga cgaccgtggc gttgtccgcg ctgcgaccga
      361 agcggaaggt cacgcgcgag ttccagacgt aggagtgcgc cgcgccgaag cagaagccga
      421 cgaccgaggc cgcgagcggc gcgatcccgt gccgccagtc gaggaccgcg acgaggaacg
      481 taaagacggc ggcatccacg gcggcgttgc cgacgccgat caacgcgaac aggacgaatt
      541 gcgtgcgcaa cgagcgaggc gcgtgacctc ggctggaggt ccccgcttcg gtggcgggcg
      601 gcgggagagg ggcgtcgctc atcgggcggc ggcgggcgag tgcatgactg tcatcatgcc
      661 ccattcgcga agcgaggcgg catcgaggca cagccgccga tgcgccccac ggtagcagcc
      721 gggtcggttc ggagcgacct cgaagctcag ttggtgttct ggaaggccca gtccatgagc
      781 ttcgccgcgt catcgtagcg gccatcgtcg ttcagcagga cgacgaagat gcggcggccg
      841 ttcttcgtgg ctgaggcgac gagggtgcgg ccggcggcgt ctgtgaaacc ggtcttcacg
      901 ccgtcggcgc cggggtaact cgataggaag ccgttgacgt ttggaaggtt gatcgtgcgc
      961 gaacccttcg cggtccactg cggggtggag acgaccctgg cgaagacggg aaggctcatc
     1021 ccgtacttcg cgacctgggc gaggtcatag gcagacatga cgtggccggc ggcatccagg
     1081 ccgtgcgggt tcacgaagtg gctgtccttg aggccgaggc gggtgaggaa ggcgttcatc
     1141 tggtcgacga aggcatcgtc cgaacctgcg atgtgacgtg cgatggccaa ggcggcgtcg
     1201 ttgccggagg gaagcagcag gccgtagacg aggtcgcgca gctggaagca gtcgccgggc
     1261 agcagtccca tcagcgagct gcccgccatc tggcggctgt cgacatcggt cttcacccag
     1321 gcatcgaggt ctccctgctc gatggcgagg atggcggtga ccatcttcgt gaggctcgcg
     1381 gggggcagcg cgaggtgcgc gtccttgtcg aagagggtct tgcccgaggc gccgtcgagc
     1441 acgagcgcgg tcttcgcggc gatcgccggg gcggcggcgg tgacggctga cgcctcgatc
     1501 gtggaggctg cggcatcggg ccctccggcg ccgccctgtg cggtggcgct cgtcgagagt
     1561 gcggaggcgg tgggcccttc gcacacgatg aggacagcgg tgcccgctgg cacctggccc
     1621 gccgcaaaac gggtgagcca tggctggttg acgacgtccg gcgcgccata gatgtagacg
     1681 acgaactcgc cagtggggtt ggtcacccag acggcggaga ggtcgcagcc gcccgaggcg
     1741 gcggtggtgg cgatgtcatc cgttgagccg ccgcgccaga cgacgaggcc gaagccaccg
     1801 gccgctggca cctcgccgcc ggtgaagccg cgcgtggtcg tcgagacctg ctgcgcgggg
     1861 gcggcgggta cgtcgacaac atctcgctcg aaggctcac
//
LOCUS       DAORPG010000415         1893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7849_length_1893_cov_2.965180, whole genome shotgun sequence.
ACCESSION   DAORPG010000415 DAORPG010000000
VERSION     DAORPG010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7849_length_1893_cov_2.965180"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLX85_08630"
     CDS             <1..222
                     /locus_tag="PLX85_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020479219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="t4-like baseplate wedge"
                     /protein_id="HRC63269.1"
                     /translation="GFNIKDAEGNPVGEIAWSDLFNVIRDTPGVRKMGDARLDLTLNG
                     LPADVRLNVREFPVLRTVTLVNGDTGELL"
     gene            222..770
                     /locus_tag="PLX85_08635"
     CDS             222..770
                     /locus_tag="PLX85_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63270.1"
                     /translation="MAILNPSFEDAGPLPGEAEHWALSAVTSLEEIAGFGTAPEEAWE
                     DFERWFELLDSIDDVVVVLAFFDSALKGYEEFESGWANVVYLYDLPPAQLVTATFDGL
                     AAEECETGWSNVPYAREWADVTAATGVFDGEPREDFEDQWRSNQLYAWTWAAVTSSTA
                     MFDAGAQAVEDFNNGWTPATTQ"
     gene            781..1476
                     /locus_tag="PLX85_08640"
     CDS             781..1476
                     /locus_tag="PLX85_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020479006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63271.1"
                     /translation="MAEADWTYLNDGLDIATVDRGVTAGIARPPGGGSFLYAFNSLSA
                     VEGAVGLFANLASFAPMAKGGSIRGVVQRGPGGGPTGFSPFLFLCCQGNSVNDSAYLL
                     GLSDDDPHRIVLRKGAVTVGLPTADGPGVLLKSAASFAQATWLHLRLDVIVNTNGDVV
                     LKVFQNDLALHALGTPPDWQPVSGMVEFIDDHLGINSGSQPLTSGRGGFGFSVKDVTR
                     RAYFDHLELFRQV"
     gene            1480..1869
                     /locus_tag="PLX85_08645"
     CDS             1480..1869
                     /locus_tag="PLX85_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63272.1"
                     /translation="MALTAFTSRLGRGQGRLATSKATGGDYAFVLGDAEPGRLFELAP
                     GDHAEVTQQTDLTGVMLVRALLRLRVPASTPPGLAWEASIIVDGTKLASMRAKPGRER
                     LVTDLAANVSKLSGLHTAGVRLELVTA"
BASE COUNT          277 a          669 c          659 g          288 t
ORIGIN      
        1 gggttcaaca tcaaggacgc cgagggcaac ccagtcggcg agatcgcctg gtcggacctc
       61 ttcaacgtca tccgcgacac gccgggcgtg cggaagatgg gcgacgcgcg tctcgacctg
      121 acgctcaacg gcctgcctgc cgacgtgcgc ctcaacgtgc gcgagttccc ggtgctgcgg
      181 accgtgacgc tggtgaacgg cgacactggg gagctgctct gatggcgatc ctcaacccca
      241 gcttcgagga tgcggggccg ctgcccggcg aggccgagca ctgggcgctc tcggcggtga
      301 cgagcctcga ggagatcgcg ggcttcggca ccgcgcccga ggaggcatgg gaggacttcg
      361 agcggtggtt cgagttgctc gactccatcg acgacgtggt cgtcgtgctc gcgttcttcg
      421 acagcgcgct caagggctac gaggagttcg agagcggctg ggccaacgtc gtctacctct
      481 acgaccttcc gcctgcgcag ctcgtcaccg cgaccttcga cgggctcgcc gccgaggagt
      541 gcgagacggg gtggagcaac gtcccctacg cgcgcgagtg ggccgacgtc accgccgcga
      601 cgggggtctt cgacggcgag ccgcgcgagg acttcgagga ccagtggcgc agcaaccagc
      661 tctacgcctg gacgtgggcg gcggtcacct cgagcaccgc gatgttcgac gcgggcgcgc
      721 aggccgtcga ggacttcaac aacgggtgga cgcccgcgac gacgcagtga ggagcaagcc
      781 atggccgaag cagactggac gtacctcaac gacgggctcg acatcgcgac ggtggaccgg
      841 ggcgtgaccg cgggcatcgc gcgcccaccg ggcggcggca gcttcctcta cgccttcaac
      901 tcgctctcgg ccgtcgaggg agcggtgggg ctcttcgcca acctcgccag cttcgctccg
      961 atggccaagg gcggctccat tcgaggcgtc gtgcagcgtg gcccgggcgg cggccccacc
     1021 ggcttctcgc cgttcttgtt cctctgctgc cagggcaact cggtcaacga cagcgcgtac
     1081 ctgctcggcc tctccgacga cgatccgcac cgcatcgtgc tccgcaaggg cgcggtgacg
     1141 gtcggcctgc ccacggccga cgggcccggc gtgctgctca agtcggcggc gtcgttcgcg
     1201 caggcgacct ggctccacct gcgcctcgac gtcatcgtga acaccaacgg cgacgtcgtc
     1261 ctcaaggtct tccagaacga cctcgcgctg cacgcgctcg gcacgccgcc cgactggcag
     1321 cccgtgtccg gcatggtgga gttcatcgac gaccacctcg gcatcaactc cggctcgcag
     1381 ccgctcacgt cggggcgcgg cggcttcggc ttctccgtga aggacgtcac gcggcgcgcg
     1441 tacttcgacc acctcgagct gttccggcag gtgtgagcga tggcgctgac cgcgttcacc
     1501 agccgtctcg ggcgcggaca ggggcgcctc gcgacgtcga aggcgacggg cggcgactac
     1561 gcctttgtcc tcggcgatgc cgagcctggg cgcctcttcg agctcgcgcc cggcgaccac
     1621 gccgaggtca cgcagcagac ggaccttacc ggcgtgatgc tggtgcgcgc gctcttgcgg
     1681 ttgcgcgtgc ccgcgtcgac ccctccgggg ctcgcgtggg aggccagcat catcgtcgat
     1741 ggcaccaagc tcgcgtccat gcgcgccaag cccggccgcg agcgtctcgt caccgacctc
     1801 gccgcgaacg tctcgaagct ctcgggcctg cataccgctg gggtgcggct cgagctggtg
     1861 accgcgtgag gagcccggca tgagcaccat cga
//
LOCUS       DAORPG010000416         1892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7858_length_1892_cov_3.420250, whole genome shotgun sequence.
ACCESSION   DAORPG010000416 DAORPG010000000
VERSION     DAORPG010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7858_length_1892_cov_3.420250"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..883)
                     /locus_tag="PLX85_08650"
     CDS             complement(230..883)
                     /locus_tag="PLX85_08650"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC63273.1"
                     /translation="MAKPVGRRGRPAHPDLLTPAEWRVLRLLQHGLSDRQIARARGTT
                     LGAVKFHRRNIAAKLALAGRPALRFWRPRAAPGRLEEGVSMSPDAATLLSVGQVSLEA
                     ADIDRTVTFFRDTLGLTHLFTFPHPTGHLAFFDCGGLRLFVTSHDDRAPEKSSVIYFR
                     VADIQAAYDALDARGVRFEGAPHLIHRHDSGVEEWMAFFRDPDENLLALMSEVATDA"
     gene            complement(938..1669)
                     /locus_tag="PLX85_08655"
     CDS             complement(938..1669)
                     /locus_tag="PLX85_08655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HRC63274.1"
                     /translation="MSSERDSATVAGQLIEFETVRSPRRRRTIELSVGAGGRVRVSAP
                     VWTSQAEIVALVGRRADWIARQRERLTQQAPPLEFRTGDRIPCLGRDVTLEVVETARR
                     HPTAARAGEVLRLDLPHELEPATRVEAARSALAAWLRRECAAYIDGHIEWWSAAVGRT
                     PAAVRVRDQRSRWGSCAPDGTLRFNWRLAMLDPGIVDYVIVHELAHLLVRNHSPAFWE
                     HVERVLPDYRARKAALRTAGRVLPL"
BASE COUNT          303 a          605 c          741 g          243 t
ORIGIN      
        1 gggggccggg ccggcgtccg gcgtcggcgg cagaccggcg gtgagggccg cggacgggga
       61 ggcgaggagc agcgggacga gcagcaacag agcgacgacg cgccgcctgg ccaacctcag
      121 cacagattgc acccgcccct acccgccgag gatcaggata acgattccga tgcagcccgg
      181 aaagctcgct gagcggaatt gtgagggttt cctagcggta gaggcggcct caggcgtccg
      241 tggctacctc cgacatcagt gcgaggaggt tctcgtccgg atcgcggaag aaggccatcc
      301 actcctcgac tcccgagtcg tggcggtgga tgagatgcgg ggcgccttcg aagcggacgc
      361 cgcgtgcgtc gagggcgtcg taggcggcct ggatgtcggc gacgcggaag tagatgaccg
      421 acgacttctc aggcgcgcgg tcgtcgtgcg aagtgacgaa gaggcggagg ccaccacagt
      481 cgaagaacgc gagatggccc gtggggtggg ggaatgtgaa gaggtgcgtc agtcccagcg
      541 tgtcgcggaa gaacgtcacc gtgcggtcga tgtcggcagc ttcgaggctg acctggccca
      601 cggagagcag ggtggcggcg tcgggtgaca tcgagactcc ttcctcgaga cggcctgggg
      661 cggctcgcgg gcgccagaag cgcagggcgg gtcgtcccgc gagcgcgagc ttcgccgcga
      721 tattgcggcg atggaacttc acggcgccga gggtggtgcc tcgggcgcgg gcgatctggc
      781 ggtcgctgag gccgtgctgg agcaggcgca ggacgcgcca ctccgcgggc gtcaggaggt
      841 ccgggtgcgc aggcctgcct cggcgaccga ctggcttcgc catggccgga tggtacgcgc
      901 gagcgtggcg aatttcccta cccctcgtgc gcgcgtacta gagcggaagg acgcgaccgg
      961 cggtccgcaa cgccgccttg cgcgcgcggt agtcgggcag gacgcgttcg acgtgctccc
     1021 agaaggccgg cgagtggttg cgcacgagga gatgcgcgag ctcatggacg atgacgtagt
     1081 ccacgatccc cggatcgagc atggcgagac gccagttgaa acgcagggtg ccgtccgggg
     1141 cacagctgcc ccagcggctg cgctggtccc gcacgcggac cgcggcggga gtgcgtccaa
     1201 cggccgccga ccaccactcg atatggccgt cgatgtaggc ggcgcattcg cgccggagcc
     1261 aggcggcgag cgcagaccgg gcggcctcga cacgcgtcgc gggttcgagc tcgtgcggca
     1321 gatcgaggcg gaggacctcg ccggctcgag ccgccgtggg gtgccggcgc gcggtctcga
     1381 cgacctcgag ggtgacgtcc cggccgaggc aggggatgcg gtccccggtc cgaaactcga
     1441 gcggcggcgc ctgctgtgtc agccgctcac gctgacgcgc gatccaatcg gcgcggcggc
     1501 cgacgagcgc cacgatctca gcctgcgagg tccagacggg agcggacacg cgaactctgc
     1561 cgccggcccc gactgatagc tcgatggtgc gccgccgccg ggggctgcgg accgtctcga
     1621 actcgatcag ctggccggcg acggtggccg agtcgcgctc cgaggacatg gccgtgcgcc
     1681 ctccgtcccg ccgccgtcca tggtgagcga tgaggggcgg gtggtcgagg cggggaatgc
     1741 aacagagggg agcccgaagg ctcccctctg ttgcgagcgc ccgtgaaggg ctagcggctg
     1801 cggcggctca cgatgcgcgc gccgagcagc atcacagaag caacggcaac ccagagcacg
     1861 gccatgccgg ccgcgccgga cgtcgaggcc ag
//
LOCUS       DAORPG010000417         1889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7887_length_1889_cov_3.705016, whole genome shotgun sequence.
ACCESSION   DAORPG010000417 DAORPG010000000
VERSION     DAORPG010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7887_length_1889_cov_3.705016"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..581
                     /locus_tag="PLX85_08660"
     CDS             234..581
                     /locus_tag="PLX85_08660"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRC63275.1"
                     /translation="MNELQGIVRFRFHEGQVEEFKRLSRQAMEIVRAQDTGTLQYETY
                     FNADESEALVLERFRDSDALIEHGEHMAALMEPILATGTVSGELCGELNPALRALMTG
                     DDPQLFTTLFMSL"
     gene            597..1094
                     /locus_tag="PLX85_08665"
     CDS             597..1094
                     /locus_tag="PLX85_08665"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRC63276.1"
                     /translation="MTSEHHVTVEGVRLRFAAAHMATLGDELEPLHGHNYEVRCRVEG
                     GLTSDRWVIDFSVLKRLVREACEALDHRFLLQRDSRLLAIEEREEGWTVRFGGRVYSF
                     PASDVVALPIENTTAELLARWIWERVAAGLGAAGGHEHIVALAVDVEEMPGQSAGYRG
                     ALGAD"
     gene            1146..1691
                     /locus_tag="PLX85_08670"
     CDS             1146..1691
                     /locus_tag="PLX85_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63277.1"
                     /translation="MQINWEEAINRIFADHLVCPRCEQTFETLVVGYSRKPSLNAFAP
                     RHRNCPRGEECEARKLITLCAECARLERLRGTPVDASQALETYMLDCRRDLEESLDYL
                     AEYWRDEVELADEDLDRPLEDVDPEAFREESEWRHRLEEEYLMYHHEFRERSRRIPSP
                     GWRSEYVEEIRDLGYDTLLGD"
BASE COUNT          315 a          551 c          721 g          302 t
ORIGIN      
        1 accggacccc ggacagcgtg ccgctgcagg accggctgac cgaggcgcgg gagcgcacga
       61 aggtgcgcac gcgcgacgtg gacgcgctgc tgacgggcct gcacatctaa cccgtccctc
      121 ctcgaccgct gtatcgccca ttcacgaggg cagctcttga gagctgccct cgtgccgtct
      181 gaaggggtaa cgcgcgtcag agggggagag tggcacgcag gaaggcaggg cagatgaacg
      241 agctccaggg catcgtgcgg ttcaggttcc acgagggcca ggtcgaggag ttcaagcgac
      301 tctcgaggca ggcgatggag atcgtccggg cgcaagatac cggcaccctg cagtacgaga
      361 cgtacttcaa tgcggacgag tcggaggcgt tggtcctcga gcgatttcgg gactcggacg
      421 cactgatcga gcacggggag catatggcgg cactgatgga gccgatcctg gccacgggga
      481 cggtctcagg tgagctctgc ggggagttga acccggcgct gcgcgcgcta atgaccggag
      541 atgacccgca gctgttcacg acgctcttca tgtcgctcta ggggagggcg gtggccatga
      601 cctcggaaca tcatgtgacg gtggaggggg tgcggctgcg gttcgcggcc gcgcacatgg
      661 cgacgctcgg cgatgagctg gagccgctgc acgggcacaa ctacgaagtg cgctgtcgcg
      721 ttgagggtgg gctcacctcg gaccgctggg tgatcgactt cagcgtgctc aagcgcctcg
      781 tacgcgaggc gtgcgaggcg ctcgaccacc ggttcctcct gcagcgcgat tcgcggctgc
      841 tggcgattga ggagcgcgag gagggctgga cggtgcgctt cggagggcgg gtgtactcgt
      901 ttcccgcctc ggacgtggtg gcgctgccga tcgagaacac gacggcagag ctgctcgcgc
      961 ggtggatctg ggagcgggtg gcggctggcc tcggggcggc cggggggcac gagcacatcg
     1021 tggcgctggc tgtggacgtg gaggagatgc cggggcagtc cgcgggatat cgaggggcgc
     1081 tgggcgcgga ttgacacgat tcaggcccgc cgtaggatcg gtgaaacggg ctgagggagc
     1141 cgaggatgca aatcaactgg gaagaagcga tcaatcggat cttcgccgat cacctcgtgt
     1201 gccctcgatg cgagcagacg ttcgagacgc tcgtcgtggg gtactcgcgc aagccgtcgt
     1261 tgaatgcgtt tgcgccgcgg catcgcaact gccctcgcgg cgaggagtgc gaggcgcgga
     1321 agctgatcac gttatgtgcc gagtgcgccc gcctcgagcg gctgcgcggg acgccggtcg
     1381 atgcctcgca ggccctcgag acgtacatgc tggactgccg tcgggacctc gaggagtcgc
     1441 tcgactacct ggcggagtac tggcgtgacg aggtggagct ggcggacgag gatctcgacc
     1501 ggccgctgga ggacgtcgac cccgaggcat tccgcgaaga gagcgagtgg cggcaccgac
     1561 tcgaggagga gtacctgatg taccaccacg agttccgtga gcgcagccga cgcatcccct
     1621 cgccgggctg gcgctcggag tacgtggagg agatccgcga tctggggtac gacacgctgc
     1681 tgggtgacta gccgagcgcg tgccttgacg ggccgctccc ctgctggagt acgtgagcga
     1741 cgggccgcgg cgatgcggcc cgttacgtta tgtcatcctc caccaatggc ggggaggatc
     1801 tggacttcgg cttcctcggc gactttggcc atgaggccag tcgtggcgac ctcgccgtta
     1861 atggcgaggg ccaggccggg gcggacgcg
//
LOCUS       DAORPG010000418         1888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7898_length_1888_cov_3.066012, whole genome shotgun sequence.
ACCESSION   DAORPG010000418 DAORPG010000000
VERSION     DAORPG010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7898_length_1888_cov_3.066012"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PLX85_08675"
     CDS             <1..328
                     /locus_tag="PLX85_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRC63278.1"
                     /translation="LHASPSQAQQPLEIFDAHGAPVAPVNFPEELRDDPQVQAEGLFV
                     DLVHEVTGEQSVVAPVFEMSATPTQVTRPSPPLGRDTREVLIEGGWSAEEVEQLVSGG
                     VVYARD"
     gene            complement(335..1435)
                     /locus_tag="PLX85_08680"
     CDS             complement(335..1435)
                     /locus_tag="PLX85_08680"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRC63279.1"
                     /translation="MPDRIGVGIIGANVNYGWGTRAHLPALQALPEFDLVGVATTQMA
                     TARETADRYGIANAFDDAAALAAHPEVDLVVVCVRVPGHYALVKTALEAGKHVFCEWP
                     LGANTAEAAELRDLATARGVRNTVGLQARGAPAFIRLRELVRDGYIGDVLSADMYSAL
                     PGAGPRRESFAWAVDRAKGASTLSIASGHSLDALAFCLGEFAEVSAVVSTRVQRVPLE
                     GSDQMLDVTAPDHVLLSGMLEGGALVSAAIKSVPAVGTGFRFEVQGSEGTLRVTSSGG
                     AQMGDLVLEGAKRGERALEAIPLEVTDRWVPETLNGPPLNVAQLFRRLGNAINGGGPA
                     HPDFDHAVRRHRFLDALEEASASGQRVRVDLG"
     gene            complement(1511..>1888)
                     /locus_tag="PLX85_08685"
     CDS             complement(1511..>1888)
                     /locus_tag="PLX85_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63280.1"
                     /translation="AEAIRARASTIAPMSPRQFVPAAVCAVLTLVALLVPLPPAVTAL
                     LVLALVLGPAVTAFFASRGLDALGYVATGMVAALAVALIGGVVNEVRNDDYQTGVIFV
                     GTLLVGLIFTLPPVLILALLRRD"
BASE COUNT          286 a          626 c          736 g          240 t
ORIGIN      
        1 cctgcacgcc tcgccctcgc aggcacagca gccgctcgag atcttcgacg cgcacggcgc
       61 cccggtcgca ccggtgaact tccccgagga gctgcgggac gacccccagg tgcaggcaga
      121 aggcctgttc gtcgacctcg tgcacgaggt gacgggcgag cagtcggtgg tggcgccggt
      181 cttcgagatg tcggcgacgc cgacgcaggt cacccggccg tcgcctccgc tcggccgtga
      241 cacccgcgaa gtgctgatcg aaggcgggtg gagcgccgag gaggtcgagc agctcgtcag
      301 cggcggcgtg gtgtacgcgc gcgactgagg cggttcagcc caggtcgacc cgaacgcgct
      361 gtccggaggc cgaggcctct tcgagcgcgt cgaggaagcg atgccgtcgc acggcgtgat
      421 cgaaatcggg gtgcgcgggg ccgccgccgt tgatggcgtt gccgaggcgg cggaacagct
      481 gtgcgacgtt gagcggcggg ccgttcaacg tctcgggcac ccagcggtcg gtgacctcga
      541 gcgggattgc ctccagcgcg cgttcgccgc gctttgcgcc ctccaggacg aggtcgccca
      601 tctgcgcgcc gccgctggac gtgacgcgga gcgtgccctc ggagccctgc acctcgaagc
      661 ggaagccggt gccgacggcc gggacgctct tgatggcggc ggagaccagc gcgccgccct
      721 cgagcatccc gctgagcagg acgtggtcgg gcgccgtaac gtcgagcatc tggtcgctgc
      781 cctcgagggg gacgcgttgg acgcgcgtcg agacgacggc ggagacctcg gcgaactcgc
      841 ccaggcaaaa ggcgagcgcg tcgagcgagt ggcccgaggc gatgctgagc gtcgaggcgc
      901 ccttcgcgcg gtcgacggcc caggcgaagc tctcgcggcg cgggccagca cccgggagcg
      961 cggagtacat gtcggcggat agcacgtcgc cgatgtagcc gtcccggacc agctcgcgca
     1021 ggcggatgaa ggccggcgcg ccgcgtgcct gcagcccgac cgtgttgcgc acgcctcggg
     1081 cggtggccag gtcgcggagc tcggccgcct ccgcggtgtt cgccccgagg ggccactcgc
     1141 agaagacgtg cttgcccgct tcgagcgcgg tcttcacgag ggcatagtgg cccgggacgc
     1201 gcacgcacac cacgacgagg tccacctcgg gatgcgcggc gagggccgcg gcatcgtcga
     1261 aggcgttcgc gatgccgtag cggtcggcgg tttcgcgggc ggtggccatc tgcgtggtcg
     1321 ccacaccgac gaggtcgaac tcggggaggg cctgcagcgc gggcaggtgg gcgcgagtgc
     1381 cccagccgta gttcacgttc gcgccgatga tgccgacgcc gatgcggtca ggcactggtt
     1441 gctcctcgtc acaggtcgcc gggcggatgc cggacgggat gatacacccg tgggtggcgg
     1501 aatcggcgtg ctagtcgcgg cgcaggaggg cgaggatcag gaccggcggc aacgtgaata
     1561 tcaaaccgac gagcagcgtg ccgacgaaga tgacgcccgt ctggtagtcg tcgttgcgca
     1621 cctcgttcac gacaccgccg atgagcgcga ccgcaagggc ggcgaccatg ccggtggcga
     1681 cgtacccgag ggcgtcgagg ccgcgcgacg cgaagaaggc ggtcaccgcc gggccgagga
     1741 cgagcgcgag caccagcagc gcggtgacgg cgggcggcag gggcaccagc agcgcgacga
     1801 gcgtcagcac ggcgcagacc gcggcgggga cgaactgacg gggcgacatc ggggcgattg
     1861 tagaggcgcg cgcccttatg gcctcggc
//
LOCUS       DAORPG010000419         1877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7966_length_1877_cov_3.760703, whole genome shotgun sequence.
ACCESSION   DAORPG010000419 DAORPG010000000
VERSION     DAORPG010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7966_length_1877_cov_3.760703"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            425..631
                     /locus_tag="PLX85_08690"
     CDS             425..631
                     /locus_tag="PLX85_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63281.1"
                     /translation="MFDELFELFERDRGRHKNGARPGLFGRLFGHHAAAEPRDDTRRH
                     VDEDDDQRDERRRPRRRDEIDFDD"
     gene            701..1678
                     /locus_tag="PLX85_08695"
     CDS             701..1678
                     /locus_tag="PLX85_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRC63282.1"
                     /translation="MSELLPWVLFTAFVIGMLAVDLGFFHREAHEVRRREALAWSAVW
                     VGLAVVFNIGIYATRGGADGIEWTTGYLIEKSLAVDNVFVFLLIFSTFQVPAIYQHRV
                     LFWGVLGALVMRGALIAAAGVLLSTLHFVIYVFGALLIVTGIKFLRDQEHAPELNRNW
                     LVRFARRFLPTTERYEGQHFFIRRSGVLYMTPLFLVLLLIEATDLVFAVDSIPAIYAV
                     TDDPFIVFTSNVFAILGLRAMYFVLAGYLSAFRYVKPALAAILVFVGVKMLLTDIYKI
                     HPLMSLVVIVTILVVAIGASIRTNRAEQRAAPPAAERGGGLPGERFEAS"
BASE COUNT          282 a          640 c          596 g          359 t
ORIGIN      
        1 cgacggagcc gtggacgccg aggcgagcct ggtcgcctcg gcgcgttccc cctacttgtc
       61 gagccaggtg gtcggattct ggtcccccag tcgtggaacg acgaggtggg tctgagcagt
      121 ccgccccggc gcgccccgcg cagccagccc tggtacgtcg tggcttaggg gcggcattcg
      181 gcacggcacc gtaccgctgg tggtgatact tgttccagat ctcagtcagc agattcttga
      241 catgcggccc acaactcgcg acaatcattt cgtaagggga gtaaccgact cggccctggg
      301 ccgaggatcg cggtcgtcag tacgggcttc ggccccggcc gcgaatgagc gccctcgctc
      361 agcggtgaga ctttgcgcat ccagccgtgg gtgcgcgaag cgcaccggga gagagggtcg
      421 tttcatgttc gatgagctgt tcgagctgtt cgaacgggat cgcgggcgcc acaagaacgg
      481 tgctcgaccg gggctgtttg ggcgcctctt cggccatcac gcggccgccg agccgcgcga
      541 tgacactcgt cgccacgtcg atgaagacga tgaccagcgc gacgagcgac gccggccccg
      601 ccggcgcgac gaaatcgatt tcgacgacta actgatctcc gacgaagcga tgacgccgag
      661 cgcgtgctcg gcctcatcgc cacgctgaaa ggtgcatccg ttgtccgagc tgttgccctg
      721 ggtcctcttc acagccttcg tcatcggcat gctcgccgtc gacctcggct ttttccaccg
      781 cgaggcgcac gaggtgcgcc gccgcgaggc gctggcctgg tccgccgtct gggtcggcct
      841 ggcagtcgtc ttcaacatcg gcatctacgc tactcgaggc ggcgccgacg gcatcgagtg
      901 gacgacgggc tacctgatcg agaaatccct tgccgtcgac aacgtcttcg tcttcctgtt
      961 gatcttctcg acgttccagg tccccgcgat ctaccagcat cgcgtgctgt tctggggtgt
     1021 cctcggcgcc ctcgtgatgc gcggcgcgct gatcgcggcc gccggtgtcc tcctgagcac
     1081 gctccacttc gtgatctacg tctttggcgc gctgttgatc gtcacgggga tcaagttcct
     1141 gcgcgaccag gagcacgctc ccgagctgaa caggaactgg ctcgtccggt tcgcccggcg
     1201 attcctgccg acgaccgagc gctacgaggg ccagcacttc ttcattcggc ggagcggcgt
     1261 cctgtacatg acgccgctct tcctcgtgct gctgctcatc gaggcgactg acctggtgtt
     1321 cgcggtcgac tccatacccg ccatctacgc ggtgacagac gacccgttca tcgtgttcac
     1381 ctcgaacgtc ttcgcgatcc tggggttacg ggcgatgtac ttcgtcctcg ccggctacct
     1441 cagcgcgttt cgctacgtga agcccgcgct cgcggcgatt ctcgtcttcg tcggtgtgaa
     1501 gatgctgctc acggacatct acaagattca cccgttgatg agcctcgtgg tgatcgtcac
     1561 gatccttgtc gtggccatcg gcgcgtcgat ccgtacgaac cgcgcggagc aacgggccgc
     1621 accgcccgcg gcggagcgag gtggcgggct ccccggcgag cgtttcgaag catcctgaca
     1681 cgcccgatga gcgcggggag ggaggcgacg gcctccctcc ccgcgtcagg ccaccggttc
     1741 gtcacccgcc caggcgtggc gcaatccctc gacatcgatc ttggtcatct gcaacatcgc
     1801 ctgcaccacg cggccggcgc gctcagggtc ggggtccccc atcaactccc cgagcgccga
     1861 gggaatgacc tgccacg
//
LOCUS       DAORPG010000420         1877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_7970_length_1877_cov_3.196487, whole genome shotgun sequence.
ACCESSION   DAORPG010000420 DAORPG010000000
VERSION     DAORPG010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7970_length_1877_cov_3.196487"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PLX85_08700"
     CDS             complement(<1..278)
                     /locus_tag="PLX85_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012930165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRC63283.1"
                     /translation="MASDLYSVLGVKRDASEKEIRQAFRRLARQYHPDVNPGNADAEA
                     RFKEINAAYEVLSDADNRKKYDQYGDQWQHADQIEEMRRQQAARGGGFR"
     gene            455..901
                     /locus_tag="PLX85_08705"
     CDS             455..901
                     /locus_tag="PLX85_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRC63284.1"
                     /translation="MVADIVRRDPFELGGLRQMMERMFEEPFFRMPAAAAGWDEGTLP
                     VDISEAEGKLVVRASLPGFKKEDIDVQVNDGVLSIKAEHNEETETRNEKFYRRERRYG
                     SVSRRIALPGIVHDAPVSAQLKDGVLTLSIEVPEKARPKQIEIQAE"
     gene            complement(980..1750)
                     /locus_tag="PLX85_08710"
     CDS             complement(980..1750)
                     /locus_tag="PLX85_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63285.1"
                     /translation="MARATASPVAEPLAPEPSEGGPLEPDEARAAFISALKRGDPWYP
                     ALLDIVARWVAAAEDEDGVTHTYLIAGEAFDWLHLAERLVQAAGRLVPRNEAERLLVH
                     GEAPDGSDELAFARAIGPSKHRAHLNFQYGVVVEEALLLSAELELYKARPINGALEEP
                     ADVQAYERVYGRTLLELLKLYRADGGPHLAGRVRHSDWQAFTYWCSKYRFKMGEPARV
                     ASDTRKALALLSRLRTEQAPAAPAPPAEDAAVIDSTAN"
     gene            complement(1753..1833)
                     /locus_tag="PLX85_08715"
     CDS             complement(1753..1833)
                     /locus_tag="PLX85_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRC63286.1"
                     /translation="MKKVGRNELCPCGSGKKYKRCHGQGL"
BASE COUNT          278 a          563 c          726 g          310 t
ORIGIN      
        1 cggaagccgc caccgcgggc ggcctgctgg cgccgcatct cctcgatctg gtcggcgtgc
       61 tgccactggt cgccgtactg gtcgtacttc ttgcggttgt cggcgtcgga gaggacctcg
      121 taggcggcgt tgatctcctt gaagcgcgcc tcggcgtccg cgttgcccgg gttgacgtcc
      181 gggtggtact ggcgcgcgag gcggcggaac gcctgccgga tttccttctc ggaggcgtcg
      241 cgcttcacac cgaggacgct gtagaggtcg cttgccatgt gtgcatggta gcagatgtga
      301 ctcggcacgt acagaggtca tgagtcgaga ggactcaagt ctattgacat gttgtgatgg
      361 gaatcatacc ttgaggtacg tcggaggccc ctcgcgaggc ttctgacagg tcagcaacag
      421 cagtcggcac ggttcacaac agaaagagag gtgtgtggtg gccgacatcg tgcgccgaga
      481 tccgttcgag ctcggtggtc tgcgtcagat gatggagcgg atgttcgagg agccgttctt
      541 ccggatgccc gcggcggccg cgggatggga cgaggggacg ctccccgtcg acatctccga
      601 ggcggagggc aagctggtgg tccgcgcctc gctgcccggc ttcaagaagg aggacatcga
      661 cgtccaggtg aacgacggtg tgctctcgat caaggcggag cacaacgagg agaccgagac
      721 ccgcaacgag aagttctacc gtcgtgagcg ccgctacggc tcggtgagcc ggcgcatcgc
      781 gctgccgggg atcgtgcatg acgcgccggt gagcgcgcag ctgaaggacg gcgtgctcac
      841 gctgagcatc gaggtgccgg agaaggcgcg tccgaagcag atcgagatcc aggccgagta
      901 gtccgggggc ttcctcggaa cgtccgaacg ggcaggaagc cccgccagct ggcggggctt
      961 cttcgtgtgc gggcgcctgt cagttcgccg tgctgtcgat gacggctgcg tcctcggcgg
     1021 ggggagcggg ggcggcgggg gcctgttcgg tgcggaggcg ggagaggagg gcgagcgctt
     1081 tgcgggtgtc ggaggcgacg cgggcgggct cgcccatctt gaagcggtac ttggagcacc
     1141 agtaggtgaa ggcctgccag tccgaatggc ggacgcggcc ggccaggtgc ggaccaccgt
     1201 cggcacggta gagcttcagc agttcgagga gcgtgcggcc gtagacccgc tcgtaggcct
     1261 gcacgtcagc cggctcctcg agcgcgccgt tgatggggcg ggccttgtac agctcgagct
     1321 cggcggagag caggagcgcc tcttcgacga cgacgccgta ctggaagttc aggtgcgcgc
     1381 ggtgcttgct cgggccgatg gcacgggcga aggcaagctc gtcgctgccg tcgggcgcct
     1441 cgccgtggac gagcaggcgt tcggcctcgt tgcgcggcac gagccggccg gccgcctgga
     1501 ccagacgctc cgcgagatgc agccagtcga aggcctcgcc ggcgatgagg taggtgtggg
     1561 tgacgccgtc ctcgtcctcg gcagccgcga cccagcgggc gacgatgtcg agcagtgcgg
     1621 ggtaccacgg gtcgccgcgc ttgagggcgg agatgaaggc ggcgcgtgcc tcatccggtt
     1681 cgagcgggcc accctcggac ggctcaggcg cgagtggctc ggcgactggg gaggcggtcg
     1741 cgcgcgccac ggctagaggc cctgcccgtg gcagcgtttg tacttctttc cggagccgca
     1801 ggggcacagc tcgttgcggc cgaccttctt caccgtggcg gtcgcggtgc gggtcgcggt
     1861 agcgaccccg ccctgcg
//
LOCUS       DAORPG010000421         1869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8022_length_1869_cov_2.945424, whole genome shotgun sequence.
ACCESSION   DAORPG010000421 DAORPG010000000
VERSION     DAORPG010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8022_length_1869_cov_2.945424"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLX85_08720"
     CDS             complement(<1..287)
                     /locus_tag="PLX85_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63287.1"
                     /translation="MTDTTEAAGAPPRIRTSGDSEVGTYLEIASGGEAGQIFTPAPNA
                     PEAIVVLDYGSQFSMLIARRIREAGVYSELVPWDTKPEDLNHLRVKGFVLSG"
     gene            392..985
                     /locus_tag="PLX85_08725"
     CDS             392..985
                     /locus_tag="PLX85_08725"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRC63288.1"
                     /translation="MPLIPPDHIEDVVRLLLAGEVAAIPTDTVYGLAARVDDAEAIAR
                     LATLKGRDPQQPIAVLFDSLEAVAPLTDPPEVFHRLAAFWPGALTLIVPARPDAFVPL
                     LVPRGAIGLRQPDDPLTRELLRRCGGLLAVTSANLHGQPAASTAAEVVAAFGADLPVL
                     DGGPRGDAASTVIDLTEDPPRVLREGPLTAARLGLVP"
     gene            1065..1508
                     /gene="rpiB"
                     /locus_tag="PLX85_08730"
     CDS             1065..1508
                     /gene="rpiB"
                     /locus_tag="PLX85_08730"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRC63289.1"
                     /translation="MRIAIGADHAGFALKEILRAELADLGDDVVDFGTHSEDSIDYPD
                     IAIPLAHAVARAEHDFGVLICSNGVGPSIAANKVHGIRAALCHDVFSAKRARQHTDAN
                     ILCLGSWAIGRGAASEVLRAFRDATFEGGRHQRRVDKINAVDRPD"
     gene            1510..>1869
                     /locus_tag="PLX85_08735"
     CDS             1510..>1869
                     /locus_tag="PLX85_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRC63290.1"
                     /translation="MPFDWARGIDAARLAVGLARATVVTLRKSAHPIEDGTFAVQGLD
                     GALEILRDRHGVPHILAITERDAFFGQGFVHAQDRLFQMDAMRRAGAGRVSEWAGHAG
                     LEADRFMRRLGFVDIAAR"
BASE COUNT          255 a          774 c          561 g          279 t
ORIGIN      
        1 cccgacagca cgaacccctt cacccgcagg tggttgaggt cctcgggctt cgtgtcccac
       61 ggcaccagct ctgagtagac cccggcctcg cggatgcgcc gcgcgatcag catcgagaac
      121 tgcgacccgt agtcgaggac gacaatcgcc tctggcgcgt tcggcgccgg cgtgaagatc
      181 tggcccgcct cgccaccgga cgcaatctcg aggtacgtcc cgacctcgct gtcgccggaa
      241 gtgcgaatcc tgggaggcgc gcccgctgcc tcggtcgtgt cggtcacatt gcgctcgggt
      301 tcggagggga agacgccgct agggccggat cgtatcgagg ccctatagtg cggtcaatga
      361 acgcccacgc gccgttccgc cccgcacccc gatgccgctg atcccgcccg accacatcga
      421 ggatgtcgtc cgcctgctcc tcgccggcga ggtcgcggcc atcccgaccg acaccgtcta
      481 cggtcttgcc gcccgcgtcg acgacgccga ggcgatcgcg cgcctcgcga ccctgaaagg
      541 ccgcgacccg cagcagccca tcgccgtcct cttcgactcc ctcgaggcgg tcgctcccct
      601 gaccgacccg cccgaggtgt tccaccgcct cgccgcgttc tggcccggcg cgctcaccct
      661 catcgtcccc gcccgccccg atgccttcgt ccccctgctc gtcccgaggg gtgccatcgg
      721 cctccgccag cccgacgacc ccctgacccg cgagctgctc cgccggtgcg gcggcctcct
      781 cgccgtgacc agtgccaacc tccacggcca gcccgctgcc tcgactgccg ccgaggtcgt
      841 tgccgccttc ggagccgacc tccccgtcct cgacggcggc ccccgcggcg acgccgcctc
      901 caccgtcatc gacctcaccg aggacccccc gcgcgtcctc cgcgaaggcc cgctcaccgc
      961 ggcacggctc ggcctcgtgc cctgagcgcg cgtcgcgagg ccgttgagcc cgcttcgtgt
     1021 acgatcgaga ccagctcaag acgccaccac tggacgggac aggcatgcgt atcgcgattg
     1081 gcgccgacca cgccggcttc gcgctcaagg aaatcctccg cgccgagctc gccgacctcg
     1141 gcgacgacgt cgtcgacttc gggactcact ccgaggactc catcgactac cccgacattg
     1201 ccatcccgct cgcacacgcc gtcgcccgcg ccgagcacga cttcggcgtg ttgatctgct
     1261 ccaacggcgt cggcccctcg atcgccgcca acaaggtgca cggcatccgc gccgccctct
     1321 gccacgatgt cttctccgcg aagcgcgccc gccagcacac cgacgccaac atcctctgcc
     1381 tcggctcgtg ggccatcggc cgtggcgcag cctccgaggt gcttcgcgcc ttccgcgacg
     1441 ccaccttcga gggcggacgc caccagcgac gcgtcgacaa gatcaacgcc gtcgaccgtc
     1501 ccgactgagg tgcccttcga ctgggcccgc ggcatcgacg ctgctcgcct ggcggtcggc
     1561 ctggcgcgag cgaccgtcgt cactctccgc aagagcgctc acccgatcga agatggcacg
     1621 ttcgctgtcc agggcctcga tggcgccctc gagatcctgc gcgaccgcca cggcgtcccc
     1681 cacatcctcg ccatcaccga gcgtgacgcc ttctttggac agggcttcgt ccacgcgcag
     1741 gaccgcctct tccagatgga cgcgatgcga cgcgccggcg caggccgcgt ctccgagtgg
     1801 gcgggccacg ccgggctgga ggccgaccgc ttcatgcgcc ggctcggttt cgtcgacatc
     1861 gccgcccgc
//
LOCUS       DAORPG010000422         1868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8027_length_1868_cov_3.836183, whole genome shotgun sequence.
ACCESSION   DAORPG010000422 DAORPG010000000
VERSION     DAORPG010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8027_length_1868_cov_3.836183"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1868)
                     /locus_tag="PLX85_08740"
     CDS             complement(<1..>1868)
                     /locus_tag="PLX85_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRC63291.1"
                     /translation="FIPPAERTDRTVARKLKESAIAIELNRQFSKQQILEWYLNSISY
                     GGVYIGVEAAAQGYFGKPASDLTLPEAALLAGIPQQPSAYSPVSEPQAAVARQQEVLR
                     LMVEHGAITQEQADEARQAPLNIIEKPAVGVQAPHFIFGPVAAEIEQRFGPTALYDGG
                     LDIVTTLDLPLQEEAQRILEKWISQYEAVSNGHNGALNAIDPRTGEVLVYVGSRDYFR
                     QDIGGENDMNRALRSPGSTLKPFTYLTAFERGWSPSTGVLDVPLTLKDGATGKPYEVR
                     NPVKGSYQGVISAAKALGNSLNVAAVNTLLFAGIDNMVTTLRQAGFTTIDSRPNAYGP
                     ALTVGGVDVTLSDLVYGYSVLAAQGVRHGVDNNSGRTAPDRQLDPATVLKVTDSTSGE
                     VLYEYHEPQSEEVFPASFPYLISSILSDGNNECITFGVCGALSLPNGRPAAVKTGTSE
                     PFEDRTDLIGDTWAVGYTPQLVAGTWFGNSDNTPLANVFSTSVSWQAWRDFMVFAADY
                     LKLPPDQFARPDSVVERDVCFPSGKLATSLCPRQFRQKGLFAAETLQGSNPIALSDDW
                     WQSSFGSARLVLPPQLQAWSGVGSWLARNGLGGSPTPTATPRSTGTPQAQPTQGPA"
BASE COUNT          291 a          619 c          630 g          328 t
ORIGIN      
        1 gagccggccc ctgcgtcggc tgtgcctgcg gggtgccggt cgaccggggg gtggccgtcg
       61 gcgtcggcga ccccccgagc ccgttgcggg ccaaccagct cccgactccg gaccaggcct
      121 gtagctgcgg tggcagcacc agccgcgcgg agccgaagct cgattgccac cagtcgtcgc
      181 tgagggcaat cggattcgac ccctggagtg tctccgccgc gaacaggccc ttctgccgga
      241 actgccgcgg gcagagcgag gtggccagct tgcccgaggg gaagcacaca tcgcgctcga
      301 ccaccgagtc gggccgtgcg aactgatcgg gcggtagctt cagatagtcc gccgcgaaca
      361 ccatgaagtc gcgccaggcc tgccatgaca cgctggtcga aaagacgttc gcgaggggcg
      421 tgttgtccga gtttccgaac caggtgccgg cgacgagctg cggcgtgtaa ccgaccgccc
      481 acgtatcgcc gatcaggtca gtgcggtcct cgaacggctc gctcgtcccc gtcttcacgg
      541 ccgccggccg gccgttgggg agagacagcg caccgcacac gccgaacgtg atgcactcat
      601 tattgccgtc cgacaggatc gaggagatca ggtacgggaa gcttgccggg aacacttcct
      661 ccgactgggg ctcgtggtac tcgtaaagca cctcgccgct ggtcgagtcg gtcaccttga
      721 gtaccgtcgc tgggtcgagc tgccggtcgg gcgcggtccg tccgctgttg ttgtcgacgc
      781 catggcggac gccctgcgcc gccagtacgg agtacccgta cacgaggtcg ctcagggtca
      841 cgtcgacgcc gccgacggtg agcgcggggc cgtaggcgtt gggccgcgag tcgatcgtcg
      901 tgaatccggc ctgccgcagc gtggtcacca tgttgtcgat tcccgcgaac agcaaggtat
      961 tcactgcggc gacgttcaag gagttcccca gcgctttcgc ggcggagatc acgccctggt
     1021 agctcccttt gaccggattg cgcacctcgt atggcttgcc ggtcgcgccg tccttcagcg
     1081 tcagaggcac gtcgaggacg cccgtcgagg gactccaccc gcgttcgaag gcggtgagat
     1141 aggtgaaggg cttcagcgtc gatcccggcg accgcagcgc ccggttcatg tcgttctcgc
     1201 cgccgatgtc ctggcggaag tagtcacggc tgccgacgta gacgagcacc tcgcccgtgc
     1261 gcgggtcgat cgcgttcagc gcgccattgt ggccgttcga cacggcctcg tactgggaga
     1321 tccacttctc gaggatccgt tgcgcctcct cttgcagcgg gaggtcgagc gtcgtcacga
     1381 tgtcgagccc gccgtcatac agcgcggtgg gcccgaagcg ctgctcgatc tccgcggcga
     1441 cggggccaaa gatgaagtgc ggggcctgca cccccactgc tggcttctcg atgatgttca
     1501 gtggagcctg acgggcctcg tcggcctgct cctgagtgat ggcaccgtgc tccaccatca
     1561 gccgcaggac ttcctgctgc ctcgcgaccg ctgcctgcgg ctccgacacg gggctatacg
     1621 ccgagggctg ctggggaatg ccggcgagca gggcagcctc cggcagcgtc agatcgctgg
     1681 ccggcttgcc aaagtatccc tgtgccgctg cctcgacgcc gatgtacacg ccaccatacg
     1741 aaatcgagtt gagataccac tcgaggattt gctgcttcga gaactggcga ttcaactcga
     1801 tcgcaatcgc gctctccttc agcttccggg cgaccgtgcg gtctgtgcgt tcggcggggg
     1861 gaatgaag
//
LOCUS       DAORPG010000423         1864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8059_length_1864_cov_3.409066, whole genome shotgun sequence.
ACCESSION   DAORPG010000423 DAORPG010000000
VERSION     DAORPG010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8059_length_1864_cov_3.409066"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1864)
                     /locus_tag="PLX85_08745"
     CDS             complement(<1..>1864)
                     /locus_tag="PLX85_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRC63292.1"
                     /translation="ALALYLNALDGQGAHLVTVNDYLARRDAQWYGPALHMLGMRIGV
                     LQSQAAYVYTPEEVSQVAGMEHLLPVERREAYDADITYGINNEFGFDYLRDNMAQTQQ
                     EKVQRSRHFAIVDEADSVLIDEARTPLIISGPQEDDLSVYPRFARLVPTLTRDVHYTV
                     EERTRSVHLTEAGIEQLERGLGVKNIYAEENFRLTRYMEAALKAEIIYQRDRDYVVRD
                     GEVVIVDDFTGRLMEGRRWSDGLHQAVEAKEGVQIQQETVTYATITLQNYFRMYEKLA
                     GMTGTAATEAEELAEIYKLEVLVMPTHRPMVRDDLPDVVYRDTRGKFKAVVEDVAEKH
                     EQGRPVLVGTVAIETSEELSDLLRRRGIDHEVLNAKQHAREAAIVARAGQRGAVTIAT
                     NMAGRGTDIKLGDGVADLGGLHIIGTERHESRRIDNQLRGRAGRQGDPGSSRFFVSFE
                     DEIMRRFAPDWLPGLMGRLGMTDDQPLESKMVTRAIEQAQQKVEAYNFDIRKNVVEYD
                     DVMNTHRDVIYTERDKVLAGESMRDTVVTMVIEEVEALGEALLGAMPRDSEGFLAQLD
                     TIVPVEGAFEAHDFEDDTVEEILDRVVDYIEAQYGELEQETGDEGQRLIERFLLL"
BASE COUNT          252 a          570 c          700 g          342 t
ORIGIN      
        1 cagcagcagg aagcgctcga tcagacgctg gccctcgtcg ccggtctcct gctcgagctc
       61 gccgtactgc gcctcgatgt agtcgaccac gcggtcgagg atttcctcga cggtgtcgtc
      121 ctcgaagtcg tgcgcctcga aagcgccctc gacggggacg atggtgtcga gctgggcgag
      181 gaagccctcg ctgtcacggg gcatggcccc gagcagcgcc tcgccgaggg cctcaacctc
      241 ctcgatgacc atggtgacga ccgtgtcgcg catggactcg ccggcgagga ccttgtcgcg
      301 ctcggtgtag atgacgtcgc ggtgggtgtt catgacgtcg tcgtactcga cgacgttctt
      361 gcggatgtcg aagttgtagg cctcgacctt ctgctgggcc tgctcgatgg cgcgggtgac
      421 catcttggac tcgagcggct ggtcgtcggt catgccgagg cgacccatga ggccggggag
      481 ccagtcgggg gcgaaccggc gcatgatctc gtcctcgaag gagacgaaga agcgcgagga
      541 gccggggtcg ccctggcggc cggcgcggcc gcggagctgg ttgtcgatgc ggcgggattc
      601 gtggcgctcg gtgccgatga tgtgcaggcc gccgaggtcg gcgacgccgt cgccgagctt
      661 gatgtcggtg ccgcggccgg ccatgttcgt ggcgatcgtg accgcgccgc gctgcccggc
      721 gcgggcgacg atggcggcct cgcgggcgtg ctgcttcgcg ttcaggactt cgtgatcgat
      781 gccgcggcgg cgcaggaggt cggagagctc ctcggaggtc tcgatggcaa cggtgccgac
      841 gaggacgggg cggccctgct cgtgcttctc ggccacgtcc tcgaccaccg ccttgaactt
      901 gccgcgggtg tcgcggtaga cgacatccgg caggtcgtcg cggaccatcg gccggtgggt
      961 gggcatgacg aggacttcga gtttgtagat ttcggcgagc tcttccgcct cggtggcggc
     1021 ggtgccggtc atgccggcga gcttctcgta catgcggaag tagttctgga gggtgatcgt
     1081 cgcgtaggtg acggtctcct gctggatctg gacgccctct ttggcctcga ccgcctggtg
     1141 gaggccgtcg gaccagcggc ggccttccat gagccggccg gtgaagtcgt cgacgatcac
     1201 gacctcgccg tcgcggacca cgtagtcacg gtcgcgctgg tagatgatct cggccttgag
     1261 ggccgcctcc atgtagcggg tgaggcggaa gttctcctcg gcgtagatgt tcttcacgcc
     1321 caggccgcgc tcgagctgct cgatgccggc ctcggtgagg tgcaccgagc gcgtgcgctc
     1381 ctcgacggtg tagtgcacgt cgcgggtgag ggtggggacg aggcgggcaa agcgggggta
     1441 gaccgagagg tcgtcctcct gcgggcccga gatgatgagc ggcgtgcgcg cctcgtcgat
     1501 gaggacgctg tcggcctcgt cgacgatggc gaagtgccgt gagcgctgga ccttctcctg
     1561 ctgcgtctgg gccatgttgt cgcgcaggta gtcgaagccg aactcgttgt tgatgccata
     1621 ggtgatgtcg gcgtcgtaag cctcgcggcg ctccaccggc aggaggtgct ccatgccagc
     1681 gacctgcgac acctcctccg gggtgtagac gtaggcggcc tggctctgca gcacgccgat
     1741 gcgcatgccg agcatgtgca acgccgggcc gtaccattgc gcgtcacgcc tcgcgaggta
     1801 gtcgttcacg gtgacgaggt gcgcgccctg gccgtcgagc gcgttgaggt agagggcgag
     1861 ggcg
//
LOCUS       DAORPG010000424         1864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8060_length_1864_cov_3.339414, whole genome shotgun sequence.
ACCESSION   DAORPG010000424 DAORPG010000000
VERSION     DAORPG010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8060_length_1864_cov_3.339414"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1864)
                     /gene="alaS"
                     /locus_tag="PLX85_08750"
     CDS             complement(<1..>1864)
                     /gene="alaS"
                     /locus_tag="PLX85_08750"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRC63293.1"
                     /translation="LRYSADQGNFWASGDTGPCGPCSEMHYDFGLTAGCPRCADGTCH
                     PDVGCGRFLEIWNLVFMAFDRQPDGSLQPLPAQNIDTGAGLERVAWVLQDAKSVYDTD
                     QLRRILAKVEELAPEYRYDPVADPDTARAMRSITEHARAIAFLVSDGVLPGNEGRGYV
                     LRRVLRRAVYFGYTIGLREAFLEQVVDAAIEASLTAHPELAPQRAFIKRVAAREEARF
                     QQVLARGLELLEQVFEREATTKRIPGRDMFTLYDTHGLPPELTVEVAGQRGFTVDREA
                     FEAEMAAQRVRSRGDGTFEDYDDPAAERYAALGVTSAFVGYEQLETRSTISAIVAEGL
                     NVERLTAGEAGSVVLPATSFYPEGGGQVGDRGDLVTPDGRFLVEDTQRYGEAIAHLGQ
                     VVEGHIAVGDAVEARVGPEWRNGSARNHTGTHLLHAALRQVLGEHVRQSGSYVGPERL
                     RFDYTHPESPTPEQLREVQRLVNGRVRDDIERETVELPYEDAIARGAIAFFEDRYTAN
                     VRMVEYCEAHAHNPEHGHSHGECFSRELCGGTHLHSTGGVGMLVIVSDSSIGAGLRRI
                     EAVTGPEAEHYVEERLDLVDSLMQRFRVPADEVLRRLDSLETQLAEERRRGEQLA"
BASE COUNT          272 a          600 c          711 g          281 t
ORIGIN      
        1 agcgagctgc tcgcccctgc gacgttcctc cgcgagctgc gtctcgaggg agtccaggcg
       61 gcgcaacacc tcgtcggcgg ggacacggaa gcgctgcatg agcgagtcga cgaggtcgag
      121 gcgctcctcg acgtagtgct ccgcctcggg gccggtcacg gcctcgatgc ggcggagtcc
      181 ggcgccgatg ctggagtcgc tgacgatcac gagcatgccg acaccgcccg tcgagtgcag
      241 gtgcgtgccg ccacagagct cccggctaaa gcactcaccg tgggagtggc catgctcggg
      301 gttgtgcgca tgcgcctcgc agtactcaac catgcgaacg ttcgcggtgt agcggtcttc
      361 gaagaacgcg atggcgccgc gggcgatcgc gtcctcgtag ggtaattcga cggtttcgcg
      421 ctcgatgtcg tcgcggaccc ggccgttcac caggcgctgt acctcgcgga gctgctcggg
      481 ggtcggtgat tcggggtggg tgtagtcaaa gcgcaggcgc tcggggccga cgtaggagcc
      541 ggactggcgc acgtgctcgc caagcacctg gcgcagcgcg gcgtggagga ggtgcgttcc
      601 ggtatggttg cgggcgctgc cgttgcgcca ctcggggccc acgcgcgcct cgacggcgtc
      661 gccgacggcg atatgcccct ccacgacctg gccgaggtgg gcgattgcct cgccgtagcg
      721 ctgggtgtcc tcgacgagga agcgtccgtc gggggtgact agatcgccac ggtcgccgac
      781 ctggccgcca ccctcggggt agaagctcgt cgcgggcagc acgacggagc ccgcctcgcc
      841 cgccgtcagg cgctcaacgt tcaggccctc cgcgacgatg gcggagatgg tcgaccgcgt
      901 ctcgagctgc tcgtagccaa cgaaggcgct cgttacgccg agggcggcgt agcgctcggc
      961 ggccgggtcg tcgtagtcct cgaacgtgcc gtcgccgcgg gagcgtacgc gctgcgcggc
     1021 catctcggcc tcgaaggctt cgcggtcgac cgtgaagccg cgctgcccgg cgacctcgac
     1081 ggtgagctcg ggcgggaggc cgtgggtgtc atacagcgtg aacatgtcgc ggccggggat
     1141 gcgcttcgtg gtcgcttcgc gctcgaagac ctgctccagc agctcgaggc cgcgcgcgag
     1201 cacctgctgg aagcgagcct cctcgcgggc agcgacgcgc ttaatgaagg ctcgctgcgg
     1261 ggcgagctcc gggtgcgcgg tcagggacgc ctcgatggcc gcgtccacga cctgctcgag
     1321 aaacgcctcg cggaggccga tcgtgtagcc gaagtacacg gctcggcgga ggacacggcg
     1381 gaggacatag cctcggccct cgttgccggg gaggacgccg tcggagacga ggaaagcgat
     1441 ggcgcgggcg tgctccgtga tcgagcgcat cgcgcgggcg gtgtcagggt cggcgacggg
     1501 gtcatagcgg tactcgggcg cgagctcctc gacctttgcg aggatgcggc ggagctggtc
     1561 ggtgtcgtag accgacttcg cgtcctggag cacccaggcg acacgctcga ggccggcgcc
     1621 ggtgtcgatg ttctgcgcag ggagcggttg gagcgagccg tccggctggc ggtcgaacgc
     1681 catgaacacg aggttccaga tctccaggaa gcggccacag ccgacatcgg ggtgacaggt
     1741 gccatcggcg cagcgggggc agcccgcggt caggccgaag tcgtagtgca tttccgagca
     1801 gggcccacag gggccggtgt cacccgaggc ccagaagttc ccctggtcgg cggagtagcg
     1861 gagg
//
LOCUS       DAORPG010000425         1856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8121_length_1856_cov_3.274292, whole genome shotgun sequence.
ACCESSION   DAORPG010000425 DAORPG010000000
VERSION     DAORPG010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8121_length_1856_cov_3.274292"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PLX85_08755"
     CDS             <1..703
                     /locus_tag="PLX85_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRC63294.1"
                     /translation="AALGQFPDDAEAARALGTMLSGKGDASYYVQAAAAEALGRTRTP
                     GAYEVLTGVLGRPAHNDVITVGALTGFAHLRDLRAIDLLLDYTSWGRHQNARRAAVDA
                     LGALGPLADEPTRLRTRERLEGLLDDRWLRVQLSAIGALALLKDARAVPALRAVSARA
                     LEGRVRRNAQLAIRTLTEAADKGAELRGLKDDVEKLQQENQRLKDRLTKLEAAEASPP
                     TPAAPAARRARGRSS"
     gene            780..1181
                     /locus_tag="PLX85_08760"
     CDS             780..1181
                     /locus_tag="PLX85_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63295.1"
                     /translation="MRKLNVFFDVDYTLIMWDGKLRNHVEYVFQRLTEGGHTIYIWSG
                     VGIRRWDMRRHNLEGYVTDYFVKPLWGYKDRLGEYNVNVMPDFVIDDHKGVVDDFGGY
                     HIPDVADKDDRHLLNVLEAIEALAAQPPAED"
     gene            complement(1178..>1856)
                     /locus_tag="PLX85_08765"
     CDS             complement(1178..>1856)
                     /locus_tag="PLX85_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63296.1"
                     /translation="GAAGYEDGLRTTFACFGPNEEATFTLPTGEQARSDESARFLAHL
                     VVTRTLLAVGLSIDERVDAAMTTAHALSWATSGGGSYHQEPIALATSLWLIALDPESS
                     SDRPLAIDWSPACFERDWWDPDERLFSHYDIRERGLDWAAYVSYDPARHAGCSIWTIV
                     EPLLRLEQDSRARLALAQLSAASDVGQRANAAAMLERNRVAGMLRSYFGPAGSNGTTA
                     QPGWRTG"
BASE COUNT          300 a          727 c          574 g          255 t
ORIGIN      
        1 cgcggccctc gggcagttcc cggacgacgc cgaagccgcg cgtgccctcg ggaccatgct
       61 ctcgggcaag ggagacgcct cctactacgt ccaggcggcg gccgccgagg cgctcgggcg
      121 cacccgcact ccgggcgcgt acgaggttct gacgggcgtc ctcggccgtc ccgcgcataa
      181 cgacgtcatc acggtcggcg cgctcaccgg cttcgcgcat ctgcgcgatc tccgcgccat
      241 cgacctgctg ctcgattaca caagttgggg ccgccaccag aacgctcgcc gcgccgccgt
      301 cgacgccctg ggcgcgcttg gcccgctcgc cgatgagccc acccggctcc gcacccgcga
      361 acgcctcgag ggcctcctcg atgaccgctg gctccgcgtg cagctctcgg ccatcggcgc
      421 cctcgccctg ctcaaagacg cacgcgcggt tcccgccctc cgtgcggtct cagcgcgcgc
      481 tctcgaaggc cgcgtccgcc gcaacgccca gctcgccatc cgcaccctca ccgaagccgc
      541 cgacaagggc gccgaactcc gaggcctcaa ggacgatgtc gaaaagctcc agcaggagaa
      601 tcagcgcctg aaggaccgcc tgaccaaact cgaagccgcc gaggcatcgc cccccacccc
      661 ggcggcccct gccgcgcggc gcgcacgagg ccgctcctcg tgaccggcct gcccgcacgg
      721 tcgtggtacc gtcaggaacg gacgacaaac gaggtcacgc accgccaaca ggagcgacca
      781 tgcgcaagct gaacgtgttc ttcgacgtcg actacacgct catcatgtgg gacggcaaac
      841 tgcgcaacca cgtcgagtac gtcttccagc gcctcaccga gggcgggcac accatctaca
      901 tctggtccgg cgtcggcatc cgccgctggg acatgcgccg ccacaacctc gagggctacg
      961 tcaccgacta cttcgtgaag ccgctgtggg gctacaagga ccgcctcggc gagtacaacg
     1021 tcaacgtcat gcccgacttc gtgatcgatg accacaaggg cgtggtcgat gacttcggcg
     1081 gctaccacat cccggacgtt gccgacaaag acgaccgcca cctgctgaac gtcctcgagg
     1141 ccatcgaggc gttggccgcg caaccacccg ccgaggacta gcccgtccgc cagccgggct
     1201 gcgccgtcgt cccgttcgag cccgccggac cgaagtagct ccgcagcatc cccgccacgc
     1261 ggttccgctc gagcatcgcc gccgcgttcg cgcgctggcc cacgtccgat gccgccgaca
     1321 gctgcgcgag ggcaagccgc gcccggctat cctgctcgag ccgcagcaac ggctccacga
     1381 tcgtccagat cgagcagccc gcgtgcctcg ccgggtcgta gctcacatac gccgcccagt
     1441 cgaggccgcg ctcgcggatg tcataatgcg agaacaggcg ctcgtccggg tcccaccagt
     1501 cacgctcgaa gcatgccggt gaccagtcga tcgcgagcgg ccggtccgac gagctctcgg
     1561 ggtcgagcgc gatcagccac agcgaggtcg cgagggcgat cggctcctgg tggtagctcc
     1621 cgccgccgct cgtcgcccac gacagcgcat gcgcggtcgt catcgccgcg tcgacccgtt
     1681 cgtcgatcga gagccctacc gcgaggagcg ttcgcgtcac gacgaggtgc gccaggaacc
     1741 gtgccgactc gtcactgcgg gcctgctcac ccgtcgggag cgtgaacgta gcctcctcgt
     1801 tgggcccgaa gcacgcgaac gtcgtccgta gcccgtcctc gtaccctgcg gccccc
//
LOCUS       DAORPG010000426         1852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8142_length_1852_cov_2.860879, whole genome shotgun sequence.
ACCESSION   DAORPG010000426 DAORPG010000000
VERSION     DAORPG010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8142_length_1852_cov_2.860879"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..703
                     /locus_tag="PLX85_08770"
     CDS             59..703
                     /locus_tag="PLX85_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63297.1"
                     /translation="MSWTTGRYRHFVVVTGLPAEEAEPRAVELAVVQQGRFEHPTPLT
                     DADLDDSQSGLVRAGGIPVYWVGPTWTLMTGPAAKLARSDVLLRPGSGPGYGASLEYG
                     ATDSPPGAFDIGTWSTTQWEAMLAGPLGPMALPGCAPPRVIALADRRIEVHGMGHAPD
                     CFSSASAYVFFDDVVVSILFPHCFACQPQPSAYSTPAYLEEVARALRRYEAGSR"
     gene            1055..1852
                     /locus_tag="PLX85_08775"
                     /pseudo
     CDS             1055..1852
                     /locus_tag="PLX85_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941432.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
BASE COUNT          293 a          572 c          690 g          297 t
ORIGIN      
        1 gtgagacgcg gctggcgtga cggatgagcc gggacgcgcc gaacggtccg ggtccgcgtt
       61 gagctggacc acgggccgct accgccactt cgtggtggtc acgggactcc ccgccgagga
      121 ggcggagccc cgggcggtgg agctggcggt ggtacagcag ggccgcttcg agcatccgac
      181 gccgctgacc gacgcggacc tcgatgattc gcaatctggc ctcgtgcgag cgggtgggat
      241 ccccgtgtac tgggtggggc cgacctggac gctgatgacg ggaccggcgg cgaaactcgc
      301 acgttcagac gtactgctga ggccggggag cgggcccggg tacggcgcga gtctcgagta
      361 cggggccacg gactctccgc cgggcgcgtt cgacatcggg acatggtcga ccacgcagtg
      421 ggaggcgatg ctcgcggggc cgctcggccc gatggcgttg ccgggctgcg ccccaccccg
      481 ggtgatcgcc ctcgcagacc ggcgaatcga ggtccatggg atgggtcacg cgccggactg
      541 cttctccagc gcctcggctt atgtcttctt cgatgatgtg gtggtgtcga tcctgttccc
      601 gcactgcttc gcgtgccagc cgcagccgtc ggcgtacagc acgccggcgt acctcgagga
      661 ggtggcgcgg gcgctgcgac ggtacgaggc cggctcgcgc tgagtgcgga ccggcggggc
      721 gtgtcgtgta gaagaactgt tgacggtgga gatcgggact tctatgcttg agtttctgcc
      781 tcgacacggg taccatttgt aggcgttggg cgctgcgttt ccactgcggg acgagatcgt
      841 aggtgccacc tcggcgaagc gagagcatgc cggggtggct tttggtgtct ctgtcacgca
      901 ctcctcgtgg cggcgtccgg cgtggccgtt ccccggcagg cagacgtagc gaatagtccc
      961 ggaggagtcg aatttgacca ccgaccggcg cattgtgcgc tccattcgcc gcatccccga
     1021 gaccgtgagg cagtacgggc gggcgggcag cgtcctggac gtgcccgacc tgatcgagat
     1081 gccgcggcac tcctacgatc ggttcatccg cgaggggctg aaggagctct tcgaggagat
     1141 ctcgccgatc gtcgacttta ccgggacgcg catggagttg cgcttcgggg agtaccggct
     1201 cgaggagccg aagtacaccg agcgtgagtg ccggcagcgc gacgcgacgt acgcggcgcc
     1261 gctgcgggtg cgctcggagc tgctgatcaa agagacaggg gagatcaagg agcaggacct
     1321 gttcctgggc gacctgcccc tgatgaccga ccagggcacg ttcatcatca acggcgccga
     1381 gcgcgtggtg gtatcgcagc tcgtgcgctc gccgggcgtg tacttcaccg gggtcaccga
     1441 cccggcgacc ggccgccagt tgacggcggc gaagctgatc ccgaaccggg gggcgtggct
     1501 ggagttcgag acctcgaaca aggacgtgct gtccgtgaag gtcgaccgca agcgcaagat
     1561 cccggtctcg gtgctgctgc gctgcgtgga catggaagag gggatcgcgg aggaggagct
     1621 cgggacgaac gagcgcatcc tcgcgtggtt cgccgacatc gacacgaacg cggaccacaa
     1681 gtacatcgag gcgacgctcg cgaaggactc gacggagaac cggcgccagg cgctcgagga
     1741 gttctaccgg cgcatccggc ccggcgaccc gccgacggcg gagaacgcgc ggaacctgct
     1801 cgactcgctg ttcttcagcg accgtcgcta cgacctcgga cgggtgggac gg
//
LOCUS       DAORPG010000427         1852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8143_length_1852_cov_2.760712, whole genome shotgun sequence.
ACCESSION   DAORPG010000427 DAORPG010000000
VERSION     DAORPG010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8143_length_1852_cov_2.760712"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PLX85_08780"
     CDS             <1..604
                     /locus_tag="PLX85_08780"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HRC63298.1"
                     /translation="SANPQEGKSTSVANLAVVLAQAGQRVIVVDTDLRRPFLHTFFQV
                     PNSFGLTGLLLTEAEEPDAALVATGIQNLMLLPSGPRPPNPSELLTSPAMQHMIERLG
                     TRADYVLFDSPPILAVTDASILAASTDAAILIAERGRTRTEALRRAHQALLRANARVL
                     GVVINKAKGHGSGYGYYGAYGERSDPQPDRPSQRRSITGE"
     gene            629..>1852
                     /locus_tag="PLX85_08785"
     CDS             629..>1852
                     /locus_tag="PLX85_08785"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HRC63299.1"
                     /translation="MSATAGELRVRAHTARRRVAAVARNQSVRRWAARLLIAALILFV
                     CVEAVRWVSFYRHVARAQDSLVGLEQQLDLTSLAQTEQAATDVRDRLALADADLSRAE
                     DHLRTDPLMAVARRVPVLGTQATGLAEVVRLAHATAFTARAASDVLVAYSRQHDDPTR
                     SAVQEGIAFLDAQSEAMAEVRRRLTVTEEARTDIPGGLAGPLQRRRERVERAVVRMDE
                     LVTGYEEAHALAPSLLGFDRPRTYLLLAQNDTELYPSGGLISNYGIVTFQDGQIANMR
                     FEYFVDLFRRWQQRSGNEYVAPPLPLKQYLLRDTSWALGEAGWYPDFPTTAQIASGFV
                     AKGGASARDGTIAIDLRFVEALLRVVGPVTVEDYGGVRVDADHLDEVVLEQTRSEAVL
                     PGSPGKAFLSSLAERL"
BASE COUNT          271 a          677 c          606 g          298 t
ORIGIN      
        1 cagcgccaac ccgcaggaag ggaagtccac gagcgttgct aacctcgcgg tcgtgttggc
       61 acaggccgga cagcgagtca tcgtcgtgga caccgatctc cggcgaccct tcctgcacac
      121 cttcttccag gtaccgaact cgttcggctt gaccggcctc ctgctcaccg aggctgagga
      181 acccgacgcc gcgctcgtcg cgacgggcat acagaacctc atgctgctgc cctccggccc
      241 gcggccgccg aacccgtccg aattgctgac ctcccccgcg atgcagcaca tgatcgagcg
      301 actcgggacg cgcgctgact acgtgctctt cgacagtccc cccatcctcg ctgtcacgga
      361 cgcgagcatc ctggccgcct ccaccgacgc cgccatcctg atcgcggagc gcggacggac
      421 gcggaccgag gcgctgcggc gcgcgcacca ggccttgctg cgcgccaacg cgcgcgtgct
      481 cggcgtcgtc atcaacaagg cgaagggcca cggttccggc tacggctact acggagcgta
      541 cggcgagagg tccgatccgc agccggatcg accttcgcag cgccgttcaa tcactggcga
      601 gtagcgactc gcgtccgcgg cggtttgcgt gagcgccacc gccggcgagt tgcgagtccg
      661 cgcccacacc gcgcgacgtc gggtggcggc cgtggcgcgc aaccagtcgg tgcggcgctg
      721 ggctgcgcgg ctcctgatcg ccgcgttgat cctcttcgtg tgcgtcgagg cggtgcgctg
      781 ggtttccttc taccgccacg ttgcgcgcgc gcaggactcc ctcgttgggc tcgagcagca
      841 actcgacctc acctcgttgg cgcagacgga gcaggcggcg accgatgtgc gcgaccgcct
      901 cgcgctggcg gacgccgacc tctcccgcgc cgaggatcac ctgcgcactg acccgctcat
      961 ggcagtcgcc cggcgggtgc ccgtccttgg cacgcaggcg acgggcctgg cggaagtcgt
     1021 ccgcctggcg cacgccaccg cgttcacggc gcgggcggcc tccgacgtcc ttgtcgccta
     1081 cagccgtcag cacgatgacc ccacgcgcag cgctgtccag gagggcatcg ccttcctcga
     1141 tgctcaatcg gaagccatgg ccgaggtcag gcggcggctg accgtgaccg aggaggcgcg
     1201 gacggatatc cccggcgggc tcgctggtcc cctgcagcgc cggcgagagc gcgttgagcg
     1261 cgcggtcgtc cgcatggacg aactcgtgac gggatacgag gaggctcacg cgttggcgcc
     1321 cagcctgctc gggttcgatc gcccgcgcac ctacctgctc ctcgctcaga acgataccga
     1381 gctgtacccg tcgggcggcc tcatctccaa ctacggcatc gtgacgttcc aggacgggca
     1441 gatcgcgaac atgcgcttcg agtacttcgt cgatctgttc cggcgttggc agcagcgttc
     1501 cggaaacgag tatgtcgccc ctcctcttcc gctgaagcag tacctgctcc gcgacacctc
     1561 gtgggcgctc ggtgaggccg gctggtaccc ggacttcccc acgaccgcgc agatcgcgag
     1621 cgggttcgtc gcgaagggtg gcgcctccgc ccgggacggt acgatcgcca tcgacctccg
     1681 tttcgtcgaa gccctgctgc gcgtcgtcgg acccgtgacc gtcgaggact acggaggggt
     1741 ccgagttgac gccgaccacc tcgatgaggt cgtgctcgag cagacgcgtt cggaagccgt
     1801 cctgcccggc tcgcccggaa aagcgtttct ctcctcgctc gccgaacggc tc
//
LOCUS       DAORPG010000428         1851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8148_length_1851_cov_3.291203, whole genome shotgun sequence.
ACCESSION   DAORPG010000428 DAORPG010000000
VERSION     DAORPG010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8148_length_1851_cov_3.291203"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /gene="coxB"
                     /locus_tag="PLX85_08790"
     CDS             <1..601
                     /gene="coxB"
                     /locus_tag="PLX85_08790"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRC63300.1"
                     /translation="LACPVFGIVIAAMVVALLKHRSSGPNNGDGERILGTGSLPVVWF
                     GVSSLLCAIVIIYPGLTGLFELRDHKDPDLRIVATAQMWAWTVEYPDSKVKVFGAQEL
                     VLPKDETVEVDITSIDILHSFWVPAFRQKMDAVPGQINKIYITPDRVGDRESDAAYRL
                     QCAELCGLGHAIMTMPVRVVEKAEFDAWLKQQQSVTEAK"
     gene            604..>1851
                     /locus_tag="PLX85_08795"
     CDS             604..>1851
                     /locus_tag="PLX85_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRC63301.1"
                     /translation="MTRVVRAPIVVGLVLAIIGFAFGAGVLGAIRTARGQSFWEIEVG
                     MSVGYVFALIGWLAGVGGWQHWGREWFGLEAKPYTLTGWKRYFGFSPDHKVIGIQYAV
                     TFIVVFLLSGLTAMLIRVELMRDGKQIFTPDQYNAAMGFHGITMVAVAVAVLLGGFGN
                     FIVPLMIGARDMAFPRLNALTYWFVPPVAVLLIIAVLMGGWESGWTAYPPLSSLHTKH
                     GQVLFNLAVITFGLVSILGGINFLVTIIYERAPGMTWSRLPIFVWGAFAASLISFFFT
                     QALAAALLMVLLDRIAGTSFFRQAEGGSPVLYEHVFWFYSHPAVYVMVLPGFGAALEI
                     LSHLSRKPLFAYRWVAGAMIGIVMLSGIVWAHHLFTSGMAPYLHAPFLVLTEMISIPT
                     GLIFLGAIGTIWAGKLWLQTPMLF"
BASE COUNT          266 a          665 c          537 g          383 t
ORIGIN      
        1 cctcgcctgt cccgtcttcg gcatcgtgat cgccgccatg gtggtcgcgc tgctgaagca
       61 ccgctccagc ggccccaaca acggcgatgg cgagcgcatt ctcggcaccg gctcacttcc
      121 ggtcgtctgg ttcggagtca gtagcctcct ctgcgcgatc gtcatcatct accccggcct
      181 gaccgggctg ttcgagttgc gcgaccacaa agatcccgac ctacgcatcg tcgccaccgc
      241 acagatgtgg gcgtggaccg tcgaataccc ggactccaag gtgaaggtct tcggcgcgca
      301 ggagctcgtc ctgcccaagg atgagaccgt cgaggtcgac atcacctcga tcgacatcct
      361 gcattccttc tgggtccccg cgttccgcca gaagatggac gcggttcccg gccagatcaa
      421 caagatctac atcacccccg accgcgtcgg cgaccgtgag tcggatgcgg cctaccgcct
      481 gcagtgcgcc gagctctgcg gcctgggtca cgccatcatg accatgcccg tccgcgtcgt
      541 cgaaaaagcc gagttcgatg cctggctcaa gcagcagcag agcgttacgg aggccaagta
      601 gccatgactc gagttgtgcg cgcacctatc gtcgtcggcc tcgtcctcgc gatcatcggc
      661 ttcgcctttg gcgcgggtgt gctcggcgcc attcgcaccg cccgcggaca atccttctgg
      721 gagatcgaag tcggcatgtc cgtcggctac gtcttcgcgc tcatcggctg gctcgccggc
      781 gtcggcggct ggcagcactg gggccgcgag tggttcggcc tcgaggcgaa gccgtacacg
      841 ctcaccggct ggaagcgcta cttcggcttc agcccggacc acaaggtgat cggcatccag
      901 tacgccgtca ccttcatcgt ggtcttcctg ctctccggcc tcaccgcgat gctcatccgc
      961 gtcgagctca tgcgcgacgg gaagcagatc ttcacccctg accagtacaa cgccgcgatg
     1021 ggcttccacg gcatcacgat ggtggccgtt gcagtcgctg tcctcctcgg cggcttcggc
     1081 aacttcatcg tcccgctcat gatcggcgcg cgcgacatgg ccttcccgag gctcaacgcg
     1141 ctcacctact ggttcgtccc gccggtcgcg gtcctgctga tcatcgccgt cctcatgggc
     1201 ggctgggagt ccggctggac cgcctacccg ccgctctcga gtctccacac gaagcacggg
     1261 caggtgctgt tcaacctcgc cgtcatcacc ttcggcttgg tctcgatcct cggtggcatc
     1321 aacttccttg tgaccatcat ctacgagcgc gccccgggga tgacctggag ccgtctgccg
     1381 atcttcgtct ggggcgcctt cgccgcttcg ctcatctcgt tcttcttcac gcaggccctt
     1441 gcggcggcgc tcctcatggt gctgctcgac cgtatcgccg gcacctcctt cttccgccag
     1501 gcggagggcg gcagcccggt tctgtacgag cacgtcttct ggttctactc gcaccccgcg
     1561 gtctacgtga tggtgctccc agggtttggc gcggccctcg agatcctctc gcacctgtcg
     1621 cgtaagccac tctttgccta ccgctgggtt gccggcgcga tgatcggcat cgtgatgctc
     1681 tccggcatcg tctgggcgca ccacctcttc acgagtggta tggcccccta cctgcacgcg
     1741 cccttcctgg tgctgaccga gatgatctcg attcccaccg gcctcatctt cctcggcgct
     1801 atcggcacca tctgggccgg taagctctgg ctgcagacac cgatgctgtt c
//
LOCUS       DAORPG010000429         1845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8187_length_1845_cov_3.867039, whole genome shotgun sequence.
ACCESSION   DAORPG010000429 DAORPG010000000
VERSION     DAORPG010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8187_length_1845_cov_3.867039"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1512
                     /locus_tag="PLX85_08800"
     CDS             196..1512
                     /locus_tag="PLX85_08800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC63302.1"
                     /translation="MLRKLFPNVYEGWLVTGSFAFIVVLIGSTFFYGFGTIFNPVITE
                     FGWSKASVSFGFSLRSEVQGLGAPAIGFLVDRFSARRVLIGGIVVTALGVIGMSFMQN
                     LLHFYLAMFIIAFGVSACGGPVGMVATASWFERRRARALSLMTVGGGISGLFVVFIAW
                     LVEAVGWRDGLRVMGLLIVIVGSFVALNVRSRPANHPQPIDGIAEEVDADGRTVARVD
                     NSWGVPAMQACRTKAFLMIALGQAALSFSTTALMVHTIPFMEDNGVSKSAAASAITIF
                     AVTTLVGRLGFGWLADRYDKRVLLAIGVGIVAVGMPLLAVVHSYWTGIAVLAFIAPGF
                     GGVIPVRPALLVDYFGTRHFGTINGMNMLVVTLGSFFGPWAVGLLVDRNGDYASGWLL
                     CGAVAALAVPLMFAATPPRGLVERYGAEATRRRAASAASTATAAQH"
     gene            1568..>1845
                     /locus_tag="PLX85_08805"
     CDS             1568..>1845
                     /locus_tag="PLX85_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63303.1"
                     /translation="MVRLQWWRQRPAFIAAAAAVALSLVVIGGARYASSAPAPTSTAA
                     CWNKFTGLVRVLKPNQACVPLYENPIDLGAGSGASGPVGPTGPTGATRP"
BASE COUNT          253 a          651 c          590 g          351 t
ORIGIN      
        1 gccgacatct acctcggcac cgtcacgccg taacggtcat cgaggaggcg ggaggaactc
       61 ccgcctcctc gcgaaccgcc acgcatcttt tccgtgttcg gcgagtatga gatacgtagt
      121 cagtccagca gtttgaacgc gcgcgaccgc ggcgtaacgt gttcgccctc gtgcagatga
      181 acgcgaagag gtcaggtgct ccgcaagctc ttccccaacg tctacgaagg ctggctggtc
      241 accggctcgt tcgcgtttat cgtcgtcctg atcgggtcca cgttcttcta cggcttcggc
      301 acgatcttca acccggtgat caccgagttt ggctggagca aggcctccgt ctcctttggg
      361 ttctcattgc gctccgaggt ccaggggctc ggtgcgcccg ccatcggctt cctggtcgac
      421 cgcttctccg cgcgccgagt gctgatcggc ggcatcgtcg tcacggccct cggcgtcatc
      481 ggcatgtcct tcatgcagaa cctgctgcac ttctacctcg ccatgttcat catcgcgttc
      541 ggggtgagcg cgtgcggcgg gccggttggc atggtcgcca ccgcgagctg gttcgagcgg
      601 cgtcgtgcgc gagcactgtc gctcatgacc gtcggcggcg gcatctccgg cctcttcgtc
      661 gtcttcatcg catggctggt cgaggcggtc ggctggcgtg acggccttcg agtcatgggc
      721 ctgctgatcg tcatcgtggg ctcgttcgtg gcgctgaacg tgcgttctcg gccggcgaac
      781 caccctcagc ccatcgacgg catcgccgag gaagtggacg ctgacggccg caccgtcgcg
      841 cgcgtcgaca acagctgggg cgtccccgcg atgcaggcct gccgcacaaa agccttcctg
      901 atgatcgccc tcggccaggc cgcgctctcc ttctccacga cggccctgat ggtgcacacc
      961 atccccttca tggaggacaa cggcgtctcg aagagcgccg ccgcttcagc cattaccatc
     1021 ttcgcggtga ccaccctcgt cgggaggctg ggcttcggct ggcttgccga ccgctacgac
     1081 aagcgtgtgc tgctcgccat cggcgtcggc atcgttgccg tgggcatgcc gctgctcgcc
     1141 gttgtccact cgtactggac gggtattgcc gtcctggcct tcatcgcgcc cgggttcggc
     1201 ggcgtcatcc cggtacgccc ggcgttgctc gtcgactact tcggcacgag gcacttcggg
     1261 acgatcaatg gcatgaacat gctggtcgtg acactcggat cgttcttcgg cccctgggcg
     1321 gtcggcctcc tcgtcgaccg caacggcgac tacgcgagcg gctggctgct ctgcggcgcg
     1381 gtcgccgctc tcgccgtccc gctgatgttc gcagctaccc caccccgagg cctggtcgag
     1441 cgctacggcg ccgaggcgac gcgacgccgc gcggcaagcg cggcctccac cgcaaccgcg
     1501 gcgcagcact agcgtcgtcc gactgtctct cctggttcac atcgcgaaga cgtaaaggat
     1561 cgacgccatg gtccgactgc agtggtggag acaacgaccg gcgttcatcg cggccgctgc
     1621 ggccgtcgcc ctgtcgctcg tcgtcattgg gggggcgcgc tacgccagca gcgcgccggc
     1681 gccgacgagc accgcagcct gctggaacaa gttcaccggc ctcgtgcgcg tgttgaagcc
     1741 gaaccaggcg tgtgtgccgc tgtacgagaa tccgatcgac ctgggcgccg gctccggagc
     1801 cagcgggccc gtcggtccca ccggtcccac gggcgcgacc cgccc
//
LOCUS       DAORPG010000430         1843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8206_length_1843_cov_3.558725, whole genome shotgun sequence.
ACCESSION   DAORPG010000430 DAORPG010000000
VERSION     DAORPG010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8206_length_1843_cov_3.558725"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1361)
                     /gene="hisS"
                     /locus_tag="PLX85_08810"
     CDS             complement(105..1361)
                     /gene="hisS"
                     /locus_tag="PLX85_08810"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRC63304.1"
                     /translation="MPDIRAPRGMHDLLPQQAALWRHVRDTAERVAGLFGYGLVGTPI
                     VEDASVFLRTVGDESDIVSKEMYVFEDRGGDRLALRPEGTAAVCRAYIEHGMSSLPQP
                     VRLYYFGPMFRYDRPQAGRYRQLWQFGVECIGDASPAADVEVIDLQRTLYEQLGLGEL
                     TLRVNSIGTAATRHRYIEALTEHLSPHVATLSRDSQRRFETNVLRVLDSKEDAGHPAV
                     EGAPNILDYLDEGDRRHFDEIKALLEGLGIRYEVDSRIVRGLDYYARTVWEFEPAGAG
                     GQSTVGAGGRYDGLIELLGGPPTPGTGMATGIERIMLNLGATPDLETARRPSVYIAPL
                     GENLQAALAAAREVRLGGATVRMGQSGRSLRAHFRAAESFGSSHIGIVGNNEAARGVI
                     QLKPLIADGDQIELRPSDVPEFLGAP"
     gene            1462..>1843
                     /locus_tag="PLX85_08815"
     CDS             1462..>1843
                     /locus_tag="PLX85_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRC63305.1"
                     /translation="MVVTEHVPFGVDELFATLGSYLPAERVDSIREVYAFARDSHDGQ
                     KRLSGEPYITHPVAVARLVAELHMDVPTVKAALLHDVIEDCDVTSEQLARRFSPDVAR
                     LVEGATKIDQIGPQSAASASAADAE"
BASE COUNT          286 a          631 c          614 g          312 t
ORIGIN      
        1 gttccttcat ccctggaagg catgtcccga gtatcgaggc ggctcgttgc gacgatgtgg
       61 cgttgtgccg gcggtcgtgt cgcttcgctg acgaggcgga acgactacgg cgcgccgagg
      121 aactccggga cgtcggacgg ccgcagttcg atctggtcgc catctgcaat gagcggtttg
      181 agctggatca cgccgcgggc cgcttcgttg ttgccgacga tgccgatgtg cgagctgccg
      241 aagctctcgg ccgcccgaaa atgggcacgc aggctacgcc ccgactgccc catccgcacg
      301 gtcgccccgc cgaggcgcac ctcgcgggcc gcagccaggg ccgcctggag gttctccccg
      361 agcggcgcga tgtagacgga tggccggcga gccgtctcga ggtccggtgt ggcgccgagg
      421 ttgagcatga tgcgttcgat gcccgttgcc atcccggtcc cgggcgtcgg tgggccaccg
      481 aggagttcga tcagcccgtc gtagcggccc cccgccccca cggtcgactg tccgcccgca
      541 cccgcaggtt caaattccca taccgtgcgc gcgtagtagt ccagccctcg cacgatgcgc
      601 gagtccacct cgtacctgat gccgagcccc tcgagcagtg ccttgatctc gtcgaagtgc
      661 cgcctgtcgc cctcgtcgag gtagtcgagg atattcggag caccctcgac cgccggatgc
      721 ccggcgtcct ctttcgagtc cagtacgcgc agcacgttgg tctcaaaccg tcgctgcgaa
      781 tccctcgaca acgtcgcgac gtgtgggctg agatgctccg tgagcgcctc gatgtagcgg
      841 tgccgcgtcg cggccgtgcc gatcgagtta acccgcaacg tcagttcgcc gaggccgagt
      901 tgctcgtaca gcgtccgttg caggtcgatg acttcaacgt ccgcggcggg cgacgcgtcg
      961 ccgatgcatt ccacgccgaa ctgccacaac tgccggtatc ggccggcctg cggccggtcg
     1021 taccggaaca tcggcccgaa atagtaaaga cgcacgggtt gaggcaggct ggacatcccg
     1081 tgctcgatgt aagcgcggca gacggcggcc gtgccttccg gtctcagggc gaggcggtcg
     1141 cctccacggt cctcgaagac gtacatctcc ttggagacga tgtcgctctc atcgccgacc
     1201 gtccgcagga acaccgacgc gtcctcgacg attggcgtgc cgacgagccc gtagccaaag
     1261 agacccgcga cgcgctcggc ggtgtcacgg acgtgccgcc agagcgctgc ctgttgaggt
     1321 aagaggtcgt gcatccctcg aggtgcgcgg atgtcgggca tgccgcgagt gtaggccggg
     1381 tgccccgggc gcgcgagcgc acggccggtc ttcgcccgcg accctgacgg cctcggcgac
     1441 gcgtgatacg ttgagcagaa catggtcgtg accgagcacg tccccttcgg cgtcgacgag
     1501 ttattcgcga cgctgggttc atatctccct gcggagcggg tggattcgat ccgcgaggtc
     1561 tacgcgttcg cgcgtgactc gcacgacggt cagaagcggc tctccggcga gccctacatt
     1621 acccatcccg tcgctgtcgc ccggctggtc gccgagctgc acatggacgt gccgacggta
     1681 aaagcggccc tgctccatga cgtgatcgag gactgcgacg tcacctcgga gcagctcgcc
     1741 aggcggttca gcccggacgt cgcccggctg gtcgagggcg ccaccaagat cgaccagatc
     1801 ggcccgcaaa gcgcggccag tgcctccgct gccgacgccg aaa
//
LOCUS       DAORPG010000431         1843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8208_length_1843_cov_2.691834, whole genome shotgun sequence.
ACCESSION   DAORPG010000431 DAORPG010000000
VERSION     DAORPG010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8208_length_1843_cov_2.691834"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1513)
                     /locus_tag="PLX85_08820"
     CDS             complement(<1..1513)
                     /locus_tag="PLX85_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HRC63306.1"
                     /translation="MTVRLRDASAYLFILTEAVAWFAVLRAIGTAVVRNAFDRLADQL
                     QPSTTFPGDGRAVDALAVARHGAEHAVGGPSLLLIVVAAFAAFYLVRALGESGFPLPL
                     SAAVGLAATLIALHVLVHLAVRNDFRVWEPSGLATALDSSFSGDVDPARFTADPDPAT
                     VREAATLVVSVVGLTALWFRFLVAGRGAVTYERALRSFSIGFAFVILAAILGQGIGSE
                     APAFVALLYFVLGALSLALAHMARTRTAADSVGRDAPLALAAFGTLGVIVLVGLLFGL
                     LAALDVQRAFDPIFNLVLTAIGRVLYYLLLPIFWFVDWVLSHVLGNSGLEFPEQFRRF
                     GEQTVQRDKDTDGKLLPGWILQSVRVLAVAAFVWILYRFSRLLFHLTRRRDLDSAVDE
                     TRSTAAPGGGISGLVRSLFGGRAQTAWSGDWLRRQPVYRLYARLVEDAHQRGVDRGPG
                     DTPIEFARRAGAHLEAPPFESIGLAFDEARYGRHVPPPEAVQSLEGRLADWEQQ"
     gene            complement(1510..>1843)
                     /locus_tag="PLX85_08825"
     CDS             complement(1510..>1843)
                     /locus_tag="PLX85_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63307.1"
                     /translation="SRSDQLVRVLEALAMIAPFTISRLALELERPGHALPAGATLIVV
                     ASLMPPDLAATLRRLRGEGHVVHVVKTSPAEWDVDLGPIPHTNLDPVMRALEADPDAA
                     FASSGAAR"
BASE COUNT          345 a          565 c          732 g          201 t
ORIGIN      
        1 gctgctgttc ccagtctgcg aggcgaccct cgagcgactg gacggcctcg ggaggtggta
       61 cgtgccggcc gtaccgcgcc tcgtcgaaag cgaggccgat cgactcaaag ggcggcgcct
      121 cgaggtgagc gccggcgcga cgggcgaact cgatcggggt gtcgccgggg cctcgatcga
      181 ccccacgctg atgagcgtcc tcgacgagcc tcgcgtagag gcggtagacg ggctggcgtc
      241 gcagccagtc gcccgaccag gccgtctggg cgcgtccacc gaagaggcta cggaccaagc
      301 cgctgatgcc gccgcccggg gcggctgtgg agcgcgtctc atcgacggcg gaatcgaggt
      361 cgcggcggcg ggtgagatgg aagagcagac ggctgaagcg atagaggatc cagacgaagg
      421 cggccaccgc gaggacgcgg acggactgga gaatccagcc cggcagcagc ttgccgtcgg
      481 tgtccttatc tcgctggacg gtctgctcgc cgaaccggcg aaactgctcg gggaactcga
      541 ggccgctgtt gccgagcacg tgcgagagga cccagtccac gaaccagaag atgggcagca
      601 gcaggtagta caggacgcgg ccgatggcgg tgaggaccag gttgaagatc gggtcgaagg
      661 cgcgttgtac atcgagcgcg gcgagcaggc cgaacaggag gcccacgagg acgatgacgc
      721 ccagggtccc gaaggccgcc agggcgaggg gagcgtcgcg gccaacggag tcagcggcgg
      781 tgcgagtgcg cgccatgtgc gcgagcgcca gggacagcgc gccgaggacg aagtagagca
      841 gagcgacgaa cgccggcgcc tcggagccga tgccctggcc gaggatggcg gcgaggatca
      901 cgaaggcgaa gccgatgctg aacgagcgga gggcgcgttc gtaggtgacc gcgccgcggc
      961 cggccacgag aaagcgaaac cagagcgcag tcaggccgac caccgagacc acgagggtgg
     1021 ctgcctcgcg cacggtagcg gggtcgggat ccgcggtgaa ccgcgcggga tcgacatccc
     1081 cgctgaagga ggagtcgagg gcagtcgcaa gacccgaggg ctcccagact cggaagtcgt
     1141 tccgcacggc gaggtgcacg agcacgtgca gcgcgatcag ggtcgccgcc aggccaaccg
     1201 ccgcactcag cggcagcggg aagccgctct cgcccagggc gcgaacgagg tagaaggccg
     1261 caaacgcggc aacgacgatc agcagcagcg atggaccacc gaccgcgtgc tccgcaccgt
     1321 ggcgcgcgac cgccagggcg tccacggcgc gaccgtcgcc cgggaacgtg gtgctcggct
     1381 gcaactggtc ggcgagacgg tcgaaggcgt tccgcacgac ggcggtaccg atggcgcgca
     1441 ggaccgcgaa ccaggcgacg gcctcggtga ggatgaagag gtaggcggac gcgtcgcgga
     1501 gacggacggt catcgtgcgg ctccggagga tgcgaaagcc gcatccggat cggcctcgag
     1561 ggcacgcatg acggggtcga ggttcgtgtg gggaatcgga ccgaggtcga cgtcccattc
     1621 ggcgggggag gtcttcacga cgtggacgac gtggccttca ccacggaggc ggcgcagggt
     1681 cgcggcgagg tcgggcggca tgagcgacgc gacgacgatc aacgtggcgc cagcgggcag
     1741 ggcgtggccg gggcgctcca gctcgagcgc gaggcgcgaa atcgtgaacg gggcgatcat
     1801 cgcgagcgcc tcgaggacgc ggacgagctg gtcggatcgg ctg
//
LOCUS       DAORPG010000432         1837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8250_length_1837_cov_3.121773, whole genome shotgun sequence.
ACCESSION   DAORPG010000432 DAORPG010000000
VERSION     DAORPG010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8250_length_1837_cov_3.121773"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1826)
                     /locus_tag="PLX85_08830"
     CDS             complement(138..1826)
                     /locus_tag="PLX85_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRC63308.1"
                     /translation="MGSESNDHFERGQEAMRRWDWAAARTAFEEALGVEETAEAHEGL
                     ARALSWLHNEQCFEQWEQAFQLYRRRGDRPRAAMAAVWLANDLIDFRGAAAVAEGWVS
                     RARRLLEGLEPRAEHAWLALWDAVSELMGTNDPVSSRDAATRAVALAREFAVADVEFT
                     GMAVEGLALVSEGRIAEGMRLVDEAAAAAVAGDVENPNMRMTILCSLMDACDRARDYE
                     RASQWAERIQEVALGWGAEEVFSACRPHYAVVLTWRGAWDEAEAELTAAVRELSAIRP
                     AMAIEGVVRLAELRMLQGRDEEAAALFAEVEHEPLAQVGRGRMALNHGDASMAVELAE
                     RYLHRIPTADRVERVCGLELLVRGRLAAGQVEAAREAAEELRAIASTTEAPLLRAAAC
                     TAEGLIASAEREYAVARRSFEEAADCFGVAHAPFETARVRLELTRALEALGFESSALR
                     EAEAALATFIELGAAGEAARARSLMAQLAGAVPPPPTGQVPSDLGLTEREAEILRLIA
                     SGLSNRAIADELVLSVRTVERHISNLYGKLGVDGRTARAAVTAHAFRLGLVQPS"
BASE COUNT          266 a          748 c          562 g          261 t
ORIGIN      
        1 gcgccttgcg tccggcgaac tcccatgccc gcgagcctat cgaggacggc ccggcgttcc
       61 atccgcgcga gcacgcaacc cgccgccaac ttcctcgcga ggagtggcgg tgaacacgta
      121 gacgcggggc cggggggtca gctcggctgc accaacccga gacgaaacgc gtgcgctgtc
      181 accgccgccc gtgccgtccg gccatcgacg cccagcttcc cgtacaggtt cgagatgtgc
      241 cgttcgacgg tgcgcacgct caggacgagc tcgtcggcaa tcgcgcggtt gctcagcccg
      301 ctggcaatca gccgcaggat ctcggcctcc cgctcggtca gcccaaggtc gctcggcact
      361 tgccccgttg gcggcggtgg cacagcgccg gccagttgcg ccatcagtga acgcgcccgc
      421 gccgcctcgc ccgccgcgcc cagctcaatg aacgtggcga gcgccgcctc cgcctcacgg
      481 agcgctgagc tctcgaaccc cagcgcctcc agcgcccgcg tcagctcgag gcgtacccgc
      541 gccgtctcaa acggcgcgtg cgcgacgcca aagcagtctg cagcctcctc gaaggaccgg
      601 cgcgcgacgg cgtactcgcg ctccgccgag gcaatcaggc cctcagcggt gcaggcggcc
      661 gcgcgcaaca gcggggcctc ggtcgttgac gcgatcgcgc gcaactcctc ggccgcttcc
      721 cgtgccgcct cgacctgacc tgccgcgagt cgcccacgca cgagcaactc gaggccacac
      781 acacgctcca cgcggtccgc ggtcgggatg cggtgcagat aacgctccgc gagttcgacc
      841 gccatcgagg catccccatg attgagagcc atacgccctc gtccgacctg ggccagtggc
      901 tcgtgctcga cctcggcgaa tagcgccgcc gcttcctcgt cgcggccctg cagcatccgc
      961 agttccgcga gccgcaccac tccctcgatc gccatcgccg gccggatcgc gctcagctcg
     1021 cgcactgcgg cggtcagctc cgcctccgcc tcgtcccagg ctccgcgcca ggtcaggacg
     1081 accgcgtaat gcgggcggca ggccgagaac acctcctcgg cgccccagcc cagcgccact
     1141 tcttgaatcc gctccgccca ctgcgaggcg cgctcgtagt cccgcgcccg gtcgcacgcg
     1201 tccatcagcg agcaaagtat cgtcatccgc atgttcgggt tctcaacgtc cccggcgacg
     1261 gccgcggcgg ccgcctcgtc gacgaggcgc atcccctccg caatccgccc ctcgctcacg
     1321 agcgccaggc cttcgacggc catcccggtg aactcgacgt ccgcgacggc gaactcccgc
     1381 gccagtgcca ccgcccgtgt ggcggcgtcg cgcgaagaca caggatcgtt tgtccccatc
     1441 agctccgaga cggcatccca caacgccagc caggcgtgct ccgccctcgg ttcgaggccc
     1501 tcgagcagcc gccgcgcccg agacacccag ccctcggcta ccgccgccgc accccggaag
     1561 tcaatgaggt cgttcgcgag ccacaccgcc gccatcgcgg cccgcgggcg gtcgcctcgt
     1621 cgccggtaga gttggaacgc ctgctcccac tgctcgaagc actgctcgtt gtggagccag
     1681 ctgagcgccc gcgccaggcc ttcgtgcgcc tccgccgtct cctcaacgcc aagcgcttct
     1741 tcgaaggcag ttcgggcagc ggcccagtcc caccggcgca tcgcttcctg gccgcgctcg
     1801 aagtggtcgt ttgactcgct gcccaccggt tactccg
//
LOCUS       DAORPG010000433         1836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8255_length_1836_cov_3.773723, whole genome shotgun sequence.
ACCESSION   DAORPG010000433 DAORPG010000000
VERSION     DAORPG010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8255_length_1836_cov_3.773723"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PLX85_08835"
     CDS             <1..766
                     /locus_tag="PLX85_08835"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRC63309.1"
                     /translation="TGSGAADDPMRGAAPPPDRDWADLWLNANLATRLALREAVDAIE
                     EPFEGRVPVELASVLPAGSTLVVGNSMPVRDMDAFFPLVPRDVRVVGTRGASGIDGVV
                     STAAGAAAAGTGPVVLTIGDLSFFHDLNGLWALKRHGLSLTVLLVNNDGGGIFHFLPQ
                     AEAAPDQFEPWFGTPHGLDFRPAVEMHGGHFETVEGTRGWETQLRTALGRAGLTVLQL
                     RTERQRNVQLHRQVWAHVARAVHASLEASRAAGVLP"
     gene            763..1608
                     /gene="menH"
                     /locus_tag="PLX85_08840"
     CDS             763..1608
                     /gene="menH"
                     /locus_tag="PLX85_08840"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HRC63310.1"
                     /translation="MTRVDVGGVFLNLEVAGSGPPVVLLHGFTGSAAGWRETVEALGT
                     AYTTVAIDIIGHGKSDAPTALDHYRMEQCVDDLVRAVRLAGYERATWFGYSMGARTAL
                     QVAARRPEAVGALILESGSPGLATAEEREARVRSDEALAERIERDGVEAFVAYWEAIP
                     LFSTQSVELRDRLRPGRVASTVTGLAHSLRGMGTGAQRALHEELGRVHVPVLIVTGEL
                     DTKFVEIGREMARVLPDATMQVVEGAGHAAHLERPQAVHSVVLDFLRGVHESRGIASG
                     DTTRV"
     gene            1630..>1836
                     /locus_tag="PLX85_08845"
     CDS             1630..>1836
                     /locus_tag="PLX85_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HRC63311.1"
                     /translation="MTEAVPANPSALRTWILAIRPPTLTAAVAPVIVGSAVAVREHAF
                     SAGPALAALIGALAIQAGANLANDL"
BASE COUNT          249 a          591 c          725 g          271 t
ORIGIN      
        1 gaccggcagc ggcgccgcgg acgacccgat gcgcggcgcc gcacctccgc cggaccgcga
       61 ctgggcggac ctctggctga acgcgaacct cgcgacacgg ctggcgctgc gcgaggcggt
      121 cgacgccatc gaggagccat tcgaggggcg cgtgccggtg gagctggcct cggtgctgcc
      181 cgccggatcg acgctggtgg tcgggaactc gatgcccgtg cgcgacatgg atgcgttttt
      241 cccgctggta cctcgggacg tgcgggtggt ggggacgcgg ggcgcatccg ggatcgatgg
      301 cgtcgtttcg acggcggcgg gcgcggcggc ggccgggacg ggaccggtgg tgctgacgat
      361 cggcgacctc tccttcttcc atgacctgaa cgggctgtgg gcgctgaaac ggcacgggct
      421 gtcgctaacg gtcctgctcg tcaacaacga tggcggaggc atctttcact tcctgccgca
      481 ggccgaggcc gccccggacc agttcgagcc gtggttcggc acgccgcacg ggctcgactt
      541 ccggcccgca gtggagatgc acggcgggca cttcgagacg gtggagggga cgcgcggctg
      601 ggagacgcag ctgcgcacgg cgctcgggcg cgcagggttg accgtgctgc agctgcggac
      661 ggagcgacag cgcaacgtgc agctgcaccg gcaggtctgg gcgcacgtcg cgcgggcggt
      721 gcatgcctcg ctcgaggcgt cgcgagccgc gggcgtgctg ccgtgacgcg tgtcgacgtt
      781 ggaggcgtat tcctgaacct cgaggtggcc ggaagcgggc cgcccgtcgt gctgctgcac
      841 ggcttcacgg gatcggccgc cggctggcgc gagacggtgg aggcgcttgg gaccgcctat
      901 acgacggtcg ccatcgacat catcggccac ggcaagtccg atgctccgac ggcgctcgac
      961 cactaccgca tggagcagtg cgtggacgac ctcgtgcggg cggtacgcct cgcgggatac
     1021 gagcgcgcga cgtggttcgg ctattcgatg ggggcgcgga cggccctgca ggtggcggcg
     1081 cggcgtcccg aggcggtagg cgcgctcatc ctcgaaagcg ggagcccggg gctcgccacg
     1141 gccgaggaac gcgaggctcg tgtgcggtcg gatgaggcgc ttgccgagcg catcgaacgc
     1201 gatggcgtcg aggcgttcgt cgcctactgg gaggcgatcc ccctgttctc gacccagtcc
     1261 gtcgagctgc gcgatcggct gaggccgggc cgcgtcgcct cgacagtgac ggggctggct
     1321 cactcactgc gggggatggg gacaggcgcg cagcgcgcac tgcacgagga gctgggccga
     1381 gtgcatgtgc ccgtgctgat cgtgaccggc gagctggaca cgaagttcgt cgagattggc
     1441 cgcgagatgg cgcgtgtgct gccggacgct acgatgcagg tggtggaggg cgcgggtcac
     1501 gccgcgcacc tcgagcgccc gcaggctgtg cactccgtcg tactcgactt tcttcggggc
     1561 gtgcacgagt ccaggggcat cgcgagcggc gacacgacga gggtctgaga tgacgatcgg
     1621 ccagggacga tgacggaggc tgtgccggcg aatccgagcg cgctgcgcac gtggatcctc
     1681 gcgatccgcc ccccgacact gacggccgcc gtggcgccgg tcatcgtcgg gtccgcggtt
     1741 gcggtgcgcg agcatgcgtt ctcggccggg ccggcgctcg cggcgctgat cggagcgctg
     1801 gcgatccagg cgggcgcaaa cctcgcgaac gacctt
//
LOCUS       DAORPG010000434         1836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8262_length_1836_cov_2.747894, whole genome shotgun sequence.
ACCESSION   DAORPG010000434 DAORPG010000000
VERSION     DAORPG010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8262_length_1836_cov_2.747894"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="PLX85_08850"
     CDS             complement(<1..1237)
                     /locus_tag="PLX85_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRC63312.1"
                     /translation="MTLARVRSGALLGIEGIPLDVEVDIGSGLPAFNIVGLPDAAVQE
                     ARERVRAAIRNAGYEFPLRRITVNLAPADVRKEGPIYDLPIAVALLVASGQIEGRFED
                     VALLGELSLDGHLRHANGVLPLVAMCRDARIATAIVPAEDVPEARLVEGIEVFGLETL
                     TQLKEHPANWPVPALAEGVSDAIEVNDLSVVQGQEHVKRALEVATAGGHNLMLQGPPG
                     AGKTLLARTIPGLLPPLTPDEALEVTKVYSVAGMLGRGHPMVVERPFRAPHHTISHAG
                     LVGGGSKIRPGEVSLAHRGVLFLDEFPEFGATALEALREPLESGSVTISRARGSATFP
                     ARFLLVAAMNPCPCGYHGDARHPCTCAEAMVTRYQRRISGPLLDRFDLFIDVPRVEYR
                     ELTAAPSGESSAAVNDAVHK"
     gene            1415..>1836
                     /locus_tag="PLX85_08855"
     CDS             1415..>1836
                     /locus_tag="PLX85_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63313.1"
                     /translation="MADLPPRYAYRVGRGEPDAWDERPDERAFSGAELATRRWMLVAA
                     GIAAAVLIVALLSVTVFGRGGGTSFGGATTTVQDAAPPAAVTTSAAAPAAGAASAGST
                     SAGDGRQLACRISRRCRPPHRGSTSCTSAGRPSRAPPP"
BASE COUNT          277 a          604 c          709 g          246 t
ORIGIN      
        1 ccttgtgcac ggcgtcgttg acggccgccg acgactcgcc cgacggggcg gcggtgagct
       61 cgcggtactc gacgcgcggg acgtcgataa acaggtcgaa gcggtcgaga agtgggccgg
      121 agatgcgccg ctgatagcgg gtgaccatcg cctcggcgca ggtgcagggg tgacgggcgt
      181 cgccgtggta gccgcaggga cacgggttca ttgccgcgac gaggaggaag cgcgcgggga
      241 aggtggcaga accgcgagca cgcgagatcg tcacgctgcc tgactcgagg ggctcgcgca
      301 gcgcctcgag agcggtggcg ccgaactcgg ggaactcgtc gaggaagagc acaccgcgat
      361 gggcgaggga gacctcgccc ggacggatct tgctgccccc gccgaccagg ccagcgtgcg
      421 agatcgtgtg gtggggggca cggaacggac gctcgacgac catggggtgg ccgcgtccga
      481 gcatgccggc gaccgagtag accttggtga cctcgagcgc ctcgtcgggg gtgaggggtg
      541 ggagcaggcc ggggatggtg cgggcgagga gcgtcttccc ggcgccgggt gggccctgga
      601 gcataaggtt gtgcccgccg gccgtcgcga cctcgagggc acgcttcacg tgttcctggc
      661 cctgtaccac cgagaggtca ttgacctcga tggcgtcaga gacaccttcg gcgagagcgg
      721 ggacgggcca gttcgccggg tgctccttga gctgagtcag cgtctcgagg ccgaagacct
      781 cgatgccctc gacgaggcgt gcctcgggaa catcctcggc gggcacgatg gcggtcgcga
      841 tgcgagcgtc gcggcacatc gcgacgagcg ggagtacgcc gttcgcgtgg cggagatgcc
      901 cgtcaagcga gagctcgccg agcagggcga cgtcctcgaa gcggccttcg atctggccgg
      961 acgcaacgag cagcgcgacc gcgatcggga ggtcgtagat cggaccctcc ttgcgcacgt
     1021 cggcgggagc gaggttcacg gtgatgcgcc ggagtgggaa ctcgtagccg gcgttgcgaa
     1081 tggcggcacg gacgcgctcg cgggcttcct gcacggcggc gtcagggagg ccgacgatgt
     1141 tgaaggcggg cagcccgctg ccgatgtcga cctcgacgtc gagggggatg ccctcgatgc
     1201 cgaggagcgc gccggagcgc acccgcgcga gcgtcaccct gccaccacga tcgtcatcgc
     1261 gaggcaagcc tacccgacac ggcgccgtcc gtctcggccc atgtcacgcg cggcctactc
     1321 tccataacga gcggccgatg gtaccctagc tgccgccgcg cgggagtgcg cggcgccgtt
     1381 ggtggcgggg tgaggacgac gaacggaggc cgtgatggcc gaccttccgc cccgctatgc
     1441 ctatcgagtc ggtcgtggcg agccggacgc ctgggacgag cgccccgacg aacgcgcctt
     1501 cagcggcgcg gagctggcga cgcgacgctg gatgctggtc gcggccggca tcgctgcggc
     1561 agtgctcatc gtggcactgc tcagcgtgac cgtgttcggc cgcggcggcg ggacatcctt
     1621 cgggggcgcg acaaccaccg tgcaggacgc tgccccgccc gccgcggtga cgacctcggc
     1681 ggcagcaccg gcagccggcg ccgcgtccgc tggaagcacc agcgcaggcg acggacggca
     1741 gctcgcgtgt aggatctcgc gccgatgccg gccaccgcat cgcggctcca cctcctgcac
     1801 ctcggctggg agaccgtccc gcgctccgcc tccgtg
//
LOCUS       DAORPG010000435         1835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8265_length_1835_cov_4.319101, whole genome shotgun sequence.
ACCESSION   DAORPG010000435 DAORPG010000000
VERSION     DAORPG010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8265_length_1835_cov_4.319101"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1531)
                     /gene="pepN"
                     /locus_tag="PLX85_08860"
     CDS             complement(<1..1531)
                     /gene="pepN"
                     /locus_tag="PLX85_08860"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HRC63314.1"
                     /translation="MTTTAPARDVLTQAEAEARAADVNGVSYELSLDLRRGAETYRGE
                     VTIRFRFDGGSDTFLDFRGKQIERFEVNGGQLDPALLWTGYRLTIPADALARDNVVRV
                     VYENEYDHTGDGLHQFIDPEDGEEYLYSNFEPYESHRLFPNFDQPDLKATYALTVVAP
                     ANWEVINNGRPRHQEETSDGRRRWVFETTRPFSTYLFALIAGPYSAVRDEHRGIPLGL
                     FCRRSLVPYLDTEELFTVTKQGLDFYEEFFDTPYPFVKYDQVFVPEFNAGAMENVGAI
                     THSERMVFRDPPTDRQRESRAEVVLHEMAHMWFGDLVTMRWWNDLWLNESFATYMSYL
                     TLTEATRFRGAWQDFNSGMKSWAYRQDQLVTTHPIAGQVPDTEHTFLNFDGITYGKGA
                     SVIKQLVAAMGMDGFREGMRIYFRRHAFQNTTLSQWLDALGEGVGRDLHEWARLWLET
                     PSLNTVAARVEPADGKIGRLTVTQMAPEEYPTIRPHRLDIALVRNDGDVARIVEVPVH
                     LD"
     gene            complement(1604..>1835)
                     /locus_tag="PLX85_08865"
     CDS             complement(1604..>1835)
                     /locus_tag="PLX85_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63315.1"
                     /translation="PYPVLGWRLLQGTHDPGDPQPPRDLPVRYWGTEVSTEPHMEYAL
                     TWFSLAAALAVIAALRLRAERRGSEGARYTAT"
BASE COUNT          278 a          634 c          601 g          322 t
ORIGIN      
        1 cgtcgaggtg cactggcacc tcgacgatac gcgccacgtc gccgtcgttc cgcaccagcg
       61 cgatgtcgag gcggtgcggc cggatggtcg gatactcctc gggcgccatc tgggtgaccg
      121 tcagtcgccc gatcttgccg tccgccggct ccacccgcgc cgccaccgtg ttcagggacg
      181 gtgtctcgag ccataggcgc gcccattcgt gcaggtcgcg gccgaccccc tcgccgaggg
      241 cgtccaacca ttgcgagagc gtcgtgtttt ggaaggcgtg gcggcggaag tagatccgca
      301 tcccctcgcg gaacccgtcc atgcccatcg cggcgacgag ctgcttgatg acggaggcgc
      361 ccttgccgta cgtgatgccg tcgaagttca ggaacgtgtg ttcggtgtcg ggcacctggc
      421 ccgcgatcgg atgcgtcgta acgagctggt cctgccggta cgcccagctc ttcatcccgg
      481 agttgaagtc ctgccacgcc cctcggaagc gagtcgcttc cgtcagggtg agatacgaca
      541 tgtacgtggc gaagctctcg ttcagccaca ggtcgttcca ccagcgcatc gtcacgaggt
      601 cgccgaacca catgtgcgcc atttcgtgca gtacgacctc ggcgcggctt tcccgctgcc
      661 ggtcggtggg cgggtcgcgg aacaccatcc gctccgagtg cgtgatcgcg cccacgttct
      721 ccatcgcccc cgcgttgaac tctggcacga acacctggtc gtacttcacg aacgggtagg
      781 gcgtgtcgaa gaactcctcg tagaagtcga gcccctgctt cgtgacggtg aacagctcct
      841 ccgtgtcgag gtacggcacg agcgacctcc ggcaaaacag gccgagcgga atgcctcgat
      901 gctcgtcgcg caccgccgag tacggcccgg cgatgagcgc gaacaggtac gtgctgaacg
      961 gccgcgtcgt ctcgaacacc cagcgccgcc gtccgtccga ggtctcttcc tggtgccgcg
     1021 gacgcccgtt gttgatgacc tcccagttcg ccggcgctac gacggtcaac gcgtaggtcg
     1081 ccttcaggtc gggctggtcg aagttgggaa acagtcgatg cgactcgtac ggctcgaagt
     1141 tggagtagag gtactcctcg ccatcctccg ggtcgatgaa ctggtgcagc ccgtccccgg
     1201 tatggtcgta ctcattctcg tacaccaccc gcaccacgtt gtcgcgggcc agcgcgtccg
     1261 ccgggatcgt gagccggtag cccgtccaga gcagcgccgg gtctagctgc cccccgttga
     1321 cctcgaagcg ctcgatctgc ttcccgcgga agtcgaggaa cgtgtcactc ccgccgtcga
     1381 accggaaccg gatggtaacc tcgccgcgat acgtctccgc gccgcgccgc aggtcgagcg
     1441 acagttcgta gctgacgccg ttgacgtcgg cggctcgcgc ttccgcctcc gcctgggtca
     1501 acacgtcgcg tgcgggcgca gtcgtagtca tcgcgagtcc cttcgttgga gcaatggtgg
     1561 gtgggtgtcc gcccggcgcc gccccccgct ggggacgtgt cggtcaggtc gcagtatacc
     1621 gagcgccctc ggagccccgg cgctctgccc gcagacgcaa cgcggcaatg accgcgagcg
     1681 ccgccgcaag gctgaaccag gtcagggcgt actccatgtg cggctcggta ctcacttccg
     1741 tgccccagta gcgcacgggg aggtcgcgtg gcggctgcgg gtcgccgggg tcgtgcgtcc
     1801 cctggagcaa ccgccagccg agcaccggat acggc
//
LOCUS       DAORPG010000436         1834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8269_length_1834_cov_3.323215, whole genome shotgun sequence.
ACCESSION   DAORPG010000436 DAORPG010000000
VERSION     DAORPG010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8269_length_1834_cov_3.323215"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="PLX85_08870"
     CDS             complement(<1..804)
                     /locus_tag="PLX85_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC63316.1"
                     /translation="MVQTMKRSRKQRIDAAIAYALDRRWDLAAAENRSLLEEFPDDLE
                     AANRLGKALTELGDLDGAAAAYQRSLELDSTNVIARRNLSRIEEMRGSAAKRRPAKRA
                     STAGAAPTPAVRPHSLIEESGKSAEFELIEPSAQGLRRASAGEPAELVPTARGISVQT
                     AGGVVLGRIETRAGLRLKRLIEGGNRYAAVIRRVSDTEGSIYVRETYRDPSLVDQPSF
                     LPPATSARRRLTPRAYTKSSIVRYGGEGLDGDEIEDDEGEGGWTPRPRRG"
     gene            complement(1044..1120)
                     /locus_tag="PLX85_08875"
     tRNA            complement(1044..1120)
                     /locus_tag="PLX85_08875"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1084..1086),aa:Pro,seq:cgg)
     gene            1292..>1834
                     /locus_tag="PLX85_08880"
     CDS             1292..>1834
                     /locus_tag="PLX85_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63317.1"
                     /translation="MPRRLTLVALLLVMTAVAALVAARPSLDAQSLGLGEENSWIRVQ
                     NLGGQPATVDVTFYDLAGHQVATDGCPDGGCGAIQPGFGWSFFQQGFGGLAGGYRGSA
                     FVTADQPFVALLARDVFKGDQFQIAGDTLKLGHGTPTLYLPVVQNNAGYASRISVQNS
                     SDGADACVQIEYWAEGASGAT"
BASE COUNT          243 a          589 c          698 g          304 t
ORIGIN      
        1 gccgcggcga gggcgcggtg tccagccgcc ttcgccctcg tcgtcctcga tctcgtcgcc
       61 gtcgaggccc tcgcccccat agcggacgat cgaggacttc gtgtaagcgc gcggcgtgag
      121 ccgacgccgg gccgaggtgg ccggcggcag gaacgagggc tggtcgacga gcgacgggtc
      181 gcggtaggtc tcgcggacgt agatggagcc ctcggtgtcg ctgacgcggc ggatgacggc
      241 ggcgtagcgg ttgccgccct cgatgaggcg cttgaggcgg aggccggcgc gggtctcgat
      301 gcggccgagg acgacgccgc ccgcggtctg gaccgagatg ccgcgcgcag tcgggacgag
      361 ctcggcgggc tcacccgctg aggcgcgacg tagcccctgc gcgctgggtt cgatcagttc
      421 gaactcagcg gacttgccgg actcctcgat aagcgagtga gggcggaccg ccggggtggg
      481 cgccgcccct gcggtcgagg cgcgtttcgc cggtcggcgc ttggcggccg acccgcgcat
      541 ctcctcgata cggctgaggt tgcggcgcgc gatgacgttg gtcgagtcga gctcgagcga
      601 gcgctggtag gccgcggcgg cgccatcgag gtcgccgagc tcggtgaggg ccttgccgag
      661 gcggttggcc gcctcgaggt cgtcggggaa ctcctcgagc agcgagcggt tctcggccgc
      721 cgccaggtcc cagcgccggt cgagagcgta ggcaatggca gcgtcgatcc gctgcttccg
      781 agagcgcttc atcgtctgaa ccatctgtgc tgtacccgcc ttctcggact gccagcgccg
      841 cccgtgacgg tcatgttctc agtccgaacg taactagaac gtccgtttcc ggagaaaggt
      901 ttctctcgga tggttgcaaa tgcgttgcaa gtgcgctgcc ggggcgcgtt cccgtgagca
      961 ggaacgtcct ggcgccggtt ggacgcggtt gcggggctcg ttccgccgga atccaccggg
     1021 gaggctctct ttcggggaga aggtggtcgg ggtgcgcaga ttcgaactgc gggcctcgtc
     1081 gtcccgaacg acgcgctcta ccaagctgag ctacaccccg ggtgcggcgc gccgtccgcg
     1141 tcgagcgagc atggtacagc ccgccttggc gttgtgtcga gacgcgcgcg acgccgccca
     1201 cgccgagcgt ggtggacacg ccgggtggcg cgggtatgat cggggcggcg ggtgcgaaag
     1261 gccgttgctg gtgtcacccg gaaggcgagg cgtgcctcgc cgcctcaccc tcgtagcgct
     1321 gctgctcgtg atgaccgctg tggcggccct cgtcgcggcg aggccgtcgc tcgacgccca
     1381 gtcgctcggt ctgggcgagg aaaactcgtg gatccgggtg cagaacctcg gtgggcagcc
     1441 cgcgacggtc gacgtgacct tctatgacct cgctggccac caggtggcaa cggacggctg
     1501 tccggacggc ggctgcggcg ccatccagcc tggcttcggc tggtcgttct tccagcaggg
     1561 gttcggcggc ctcgcgggcg ggtatcgagg gtcggccttc gtgacggcgg accagccgtt
     1621 cgtggcgctc ctcgcgcgtg acgtgttcaa gggcgatcag ttccagatcg ctggcgacac
     1681 gctaaagctc gggcacggga ctccgacgct gtacttgccg gtggtgcaga acaacgccgg
     1741 ctatgcctcg cgcatttccg tacagaactc gagtgacggc gcggacgcgt gcgtgcagat
     1801 cgagtactgg gccgagggcg cctcgggggc gacg
//
LOCUS       DAORPG010000437         1827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8313_length_1827_cov_2.924944, whole genome shotgun sequence.
ACCESSION   DAORPG010000437 DAORPG010000000
VERSION     DAORPG010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8313_length_1827_cov_2.924944"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..643)
                     /locus_tag="PLX85_08885"
     CDS             complement(104..643)
                     /locus_tag="PLX85_08885"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008314177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRC63318.1"
                     /translation="MTTTEEAQQARWRELIREVPDFPQPGVLFRDITPLLHDPDGLRS
                     ANDALAAASRGFEPTLVAGVEARGFIFGVPVAERLGLPFVPMRKPGRLPTAYASVAYE
                     LEYGQTSLELHRDPSIAGHRVLVVDDLLATGGTAEAAARLVAELGGEVAGFAFFIELT
                     ELGGRVRLGRRPVAALVSF"
     gene            complement(644..>1827)
                     /locus_tag="PLX85_08890"
     CDS             complement(644..>1827)
                     /locus_tag="PLX85_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRC63319.1"
                     /translation="GAVRAVAGALRPGGGGRTASSVGSDPHHLGWVWQFRHDGEPAFV
                     RDTLASYGLGVALKTHDGTDWMSRYDPTSNAVASAARVEAFAHFFENGGVPFHAWAVV
                     TGSDPEREARMASDVLDAGARSLFLDLEPHKGFWAGSTEAAQRFGEALRRLQPSARVV
                     TSVDARPWTIDRIPMSEFAAFSDAIAPQAYWSTFASPDNMVRYLASGEVVGPAGITPA
                     FVIAAAVRRLASYGLPVHPIGDGTATASNGWREFVDASYGAAAESISVWRFGVADPAI
                     WPLLREMAPRVLSYVVQPGDTLAAIAEKWDTSVDAIVAVNELANPNVIVAGTRLRMPY
                     GAAPHVDATRSSEEAYIVESGDTLWSIAQRFGTSVEALMQANGLSDASVLLVGQRLVL
                     P"
BASE COUNT          263 a          781 c          543 g          240 t
ORIGIN      
        1 gaggtgaccc cttggccgcc gccggcccgc catgggccga agcctcgagc ccgccggcgc
       61 cgccctcgag gtcacctcgc ttctgtcgga tcacggccag tcctcagaac gacaccagcg
      121 ccgccaccgg acgccgcccc aggcgcaccc gcccgcccag ctcggtcagc tcaatgaaga
      181 acgcgaaccc cgccacctcg ccgccgagtt ccgcgaccag ccgcgccgcc gcctccgccg
      241 tcccgccggt cgccagcagg tcgtccacca ccagcacccg gtgccccgcg atcgacgggt
      301 cgcgatgcag ctccagcgat gtctgcccgt attccagctc gtacgccacc gaggcgtatg
      361 ccgtcggcag ccgccccggc ttccgcatcg gcacgaacgg cagccccagc cgttccgcca
      421 ccggcacccc gaagatgaac ccgcgcgcct ccaccccggc gaccagtgtc ggctcgaacc
      481 ccctcgaggc cgctgccagc gcgtcgttcg ccgaccgcag gccgtccggg tcgtgcagca
      541 acggcgtgat gtcccggaac aacacccccg gctgagggaa gtccggcacc tcccggatca
      601 actcgcgcca gcgcgcctgc tgtgcctcct ctgtcgtcgt catctacggc agcaccagcc
      661 gctgcccgac gaggagcacg ctcgcgtccg acaggccgtt cgcctgcatc agcgcctcca
      721 cgctcgtccc gaaccgctgg gcgatgctcc acagcgtgtc cccactctcc acgatgtatg
      781 cctcctcact gctcctcgtt gcgtccacat gcggggccgc gccatacggc atccgcagcc
      841 gcgtccccgc cacgatcacg ttgggattgg ccagctcgtt caccgccacg atcgcgtcca
      901 ccgaggtgtc ccacttctcg gcgatcgccg cgagcgtgtc ccccggctgc acgacgtatg
      961 acagcactcg aggcgccatc tcgcgcagca gcggccagat cgctgggtcc gctaccccga
     1021 accgccacac cgagatcgac tccgccgccg ccccgtacga ggcgtccacg aactcgcgcc
     1081 acccgttcga ggcggtcgcc gtcccgtcgc cgatcgggtg caccggcagc ccgtacgacg
     1141 ccagccgtcg caccgccgcc gcgatcacga acgccggcgt gatccccgcc ggccccacca
     1201 cctcaccgct cgcgaggtag cgcaccatgt tgtccgggct ggcaaacgtg ctccagtacg
     1261 cctgcggcgc aatcgcgtcg ctgaacgccg cgaactcgct catcggaatg cggtcgatcg
     1321 tccacggccg cgcgtccacc gaggtcacca cccgcgccga gggctgcagg cggcgcagtg
     1381 cctccccgaa ccgctgtgcc gcctccgtcg accccgccca gaagcccttg tgcggctcca
     1441 ggtccaggaa caggctccgc gctcccgcgt ccagcacgtc cgaggccatg cgcgcctcgc
     1501 gctccgggtc gctccccgtc accaccgccc aggcgtggaa cggcacgccg ccgttctcga
     1561 agaaatgggc gaacgcctcg acccgcgcgg cgctcgccac cgcattcgag gtcgggtcgt
     1621 accgcgacat ccagtcggtg ccgtcgtgcg tcttgagggc gactcccagc ccgtacgacg
     1681 cgagcgtgtc ccgcacgaag gccggctcgc cgtcgtgccg aaactgccag acccacccca
     1741 ggtggtgtgg gtcgctcccc accgaggagg ccgtccgccc ccctccccct ggccggaggg
     1801 ccccggcgac ggcccgcacg gcccctg
//
LOCUS       DAORPG010000438         1825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8324_length_1825_cov_3.505650, whole genome shotgun sequence.
ACCESSION   DAORPG010000438 DAORPG010000000
VERSION     DAORPG010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8324_length_1825_cov_3.505650"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PLX85_08895"
     CDS             complement(<1..232)
                     /locus_tag="PLX85_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63320.1"
                     /translation="MRDHGHDEAQEREPLTGPDASIVVTPIEAMEVQRLADSLDRIEA
                     GEHPRLRSEDAELASLLRTAGRLRIQAEHLTDS"
     gene            complement(247..921)
                     /locus_tag="PLX85_08900"
     CDS             complement(247..921)
                     /locus_tag="PLX85_08900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRC63321.1"
                     /translation="MLPPEVERDLVDRARRRDQEAFGLLYDAYLPRLYRYCLTRVGNE
                     TDAEDLTEEIFLKVFRAIEGFQWRATGAPAADPASEDDPPPADPPSAEPGSQEPRVPF
                     AAWLFRIAHNHVISFHRRAATRGPSAELSEAIRDDRRGPAEIAEAKVAAEEVFAAVRQ
                     LPEAQRDVLLLRFASGLSVAETAEALGKQQTNVKVLQHKGIQRLKRMLSGEAEGAAAD
                     AEGRRR"
     gene            1312..>1825
                     /locus_tag="PLX85_08905"
     CDS             1312..>1825
                     /locus_tag="PLX85_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63322.1"
                     /translation="MIRKLLAGVSIAAVAAVALLLVSYNGSSAQPSEAVVASIGTTTS
                     KIGVDFYAQLQINAEDDDICLPNCNTNNLNVTASAGDFIINNPLWGPFNNMGCYDQFL
                     VPCEGQVDLRPQDVQCDSECGIPYSTIDSNGFSDELNGDFCDSLFGPGDTNIWIKNHD
                     ACYFEELGAGA"
BASE COUNT          295 a          586 c          620 g          324 t
ORIGIN      
        1 agctgtcggt caggtgctcg gcctggatgc gaaggcggcc ggcagtgcgc aggagcgacg
       61 cgagctcagc gtcttcactg cggagacgcg ggtgctcacc ggcctcgatg cggtcgaggg
      121 agtccgcgag gcgttgcacc tccatcgcct cgatgggcgt cacgacgatg gaggcgtcgg
      181 gcccggtaag gggctcccgc tcctgtgcct cgtcgtgtcc gtgatcgcgc atccgctccg
      241 ctgtctctat ctgcgacgcc cttcggcgtc cgctgccgcg ccttccgcct cgcccgagag
      301 catccgcttc agccgctgta tgcctttgtg ctggaggacc ttcacgttgg tctgctgctt
      361 gccgagggcc tccgcggtct cggcgaccga gagtccggag gcgaaccgca acaggaggac
      421 gtcccgttgc gcctcgggga gctgcctgac cgccgcgaac acctcctcag ccgcgacctt
      481 ggcttccgcg atctcggcgg gtccgcgccg gtcatcgcgg atcgcctcgg agagttccgc
      541 cgaggggccg cgggtggcgg cgcgccggtg gaacgagatc acgtggttat gggcgatgcg
      601 gaagagccag gccgcgaacg gcactcgagg ctcctggctg ccgggctcgg ctgagggcgg
      661 gtccgcggga ggcgggtcgt cctcggaggc cgggtcggcg gccggggcac cggtggcacg
      721 ccactggaac ccctcgatcg cccggaagac tttcaggaag atctcttccg tcaggtcttc
      781 cgcgtccgtc tcgttgccga ccctggtcag gcagtagcga tacagcctgg gcaggtacgc
      841 gtcgtacagg agcccgaagg cttcctgatc cctcctccgt gcgcggtcaa cgagatcgcg
      901 ctcgacctcg ggaggaagca cctcgcgagg ctcgccggtg gaggcgagac gttgagaggg
      961 ggagtggtca accaccagat gctccaggcg cggcgtgccg agtagctcgg ccggccatgc
     1021 agccgaccgc gggatcgtac tcgactcgcc gacacaggag catcttcccc ctgttcaaca
     1081 ggaagaacac gcgaggcgtg gataaagtta actcgggcgc tcgaatctgt ttatagacgc
     1141 tgttctcgta ccgggagccg cctcagtctc gtcgtttctg ggatgagcgg ttaaccttcg
     1201 tcctcgggtc gcgttgttgt gtgaagcgtg caccaggtct cgcttgcggc gggccgtgtc
     1261 gtggtgtgcg gcgaactgac ccagccaggt tcggcgagta ggaggaactg tttgattcga
     1321 aaactcctcg cgggagtgtc gattgccgct gttgcggcgg ttgcactgct gctggtgtcg
     1381 tacaacggca gctcggcaca gccgagcgag gccgtcgtcg ccagcatcgg gacgaccacc
     1441 agcaaaatcg gcgtcgactt ctacgcgcag ctccagatca acgcggaaga cgacgacatc
     1501 tgtcttccca actgcaacac gaacaacctc aacgtgacgg cgagcgcggg tgacttcatc
     1561 atcaacaacc cgctctgggg cccgttcaac aacatgggct gctacgacca gttcctcgtc
     1621 ccgtgcgagg gccaggtcga cctgcgtccg caggacgtgc agtgcgacag cgagtgcggc
     1681 atcccgtact cgacgatcga ctccaacggg ttcagcgatg agctgaacgg cgacttctgt
     1741 gacagcctgt tcggtcctgg cgacacgaac atctggatca agaaccacga cgcgtgctac
     1801 ttcgaagagc tcggcgcggg cgccg
//
LOCUS       DAORPG010000439         1819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8385_length_1819_cov_2.976190, whole genome shotgun sequence.
ACCESSION   DAORPG010000439 DAORPG010000000
VERSION     DAORPG010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8385_length_1819_cov_2.976190"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PLX85_08910"
     CDS             <1..550
                     /locus_tag="PLX85_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63323.1"
                     /translation="DWTVLTVARLVPGGEGGPRIEVVEHQAWQGVATESLLAMLADRL
                     RRVWRVRRLAIDATGLGGPIAELLTSRLGRSTVEAVTFTAERKSRLGFGLLAAVHSGR
                     LRLYRMDGSVEWAECRRQLELARAVYRESRLMQFHVDPSEGHDDYLMSLALAVWAARF
                     EGGERTARGRLPEPVTAERIPA"
     gene            547..900
                     /locus_tag="PLX85_08915"
     CDS             547..900
                     /locus_tag="PLX85_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC63324.1"
                     /translation="MISTLEMVMTPPARTAETDDRRTRQDALPEHFEYRDEGCDLFSS
                     CLGCPLPRCRYDEPGGARTMMNRVRDEEIRRLRYDVGVPVDEISVRFRVSRRTVFRVL
                     KAGGPTSTPCEERGR"
     gene            897..>1819
                     /locus_tag="PLX85_08920"
     CDS             897..>1819
                     /locus_tag="PLX85_08920"
                     /inference="COORDINATES: protein motif:HMM:NF016988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HRC63325.1"
                     /translation="MSEESLPRLLANRDADRLRRYQEYLDYYEGRRGSPPARGRERTL
                     TFNYARAMVEKGASYLVTEHRPQVVAVEDTAAGRRQGAEAERALVEAWDSNDLARLDL
                     ETEVDTAVLGDGAFKVVWDERERRVVVSAPDVQGLYAWWVGDDVRRLWRVAARYRMPS
                     EQVAERIGHTPRSPGGRLPATVDVIESWTSERFELWLQGQPVEARPNPYGEIPYVIYP
                     NVPWPKQFWGISDIEPLREPLAELNRALTQLSRILELSGNPIAVLENVEEARDIAVQP
                     GAVWEIPEQARAYLLDLLQGGGVRLHVDYVEL"
BASE COUNT          255 a          539 c          768 g          257 t
ORIGIN      
        1 tgactggacg gtgctcacgg tcgcccggct cgtgccgggc ggcgaaggcg ggcctcgcat
       61 cgaggtcgtc gagcaccagg cctggcaggg cgtcgcgacg gagtcgctgc tcgcgatgct
      121 ggcggaccgg ctgcggcggg tctggcgagt gcggcggctg gccattgatg cgaccggcct
      181 cggcgggccg atcgcggagc tgctcacgtc tcgcctcggg cggtcgaccg tggaggcggt
      241 gacgttcacg gcggagcgga agtcacgcct cggattcgga ctgctggcgg cggtgcactc
      301 ggggcggctg cggctgtacc ggatggacgg gtcggtggaa tgggcggagt gccggcggca
      361 actggagctg gcacgggcgg tgtaccgcga gtcgcggttg atgcagttcc acgtggaccc
      421 ctcggagggc cacgacgact acctgatgag tctggcgctg gcggtgtggg cagcgcgctt
      481 cgagggaggc gagcgcacgg cgcgcggccg cctcccggag ccggtgacgg cggaaaggat
      541 tcccgcatga tctcgacgct tgagatggtg atgacgccgc ccgcgcggac ggcggagacg
      601 gacgaccggc ggacacgcca ggacgcgctg ccggagcact tcgaataccg tgacgagggg
      661 tgcgacctgt tctcgtcgtg cctcgggtgc cccctgccgc gctgccggta cgacgagccg
      721 gggggcgcgc gcacgatgat gaaccgcgtg cgcgacgagg agatccgacg gctgcgctac
      781 gacgtcggtg tgccggtgga tgagatctcg gtgcgctttc gagtgtcgcg gcggacggtg
      841 ttccgcgtgc tgaaggcggg cgggccgacc tcgacaccgt gcgaggagcg cgggcggtga
      901 gcgaggagtc gctgccgcgg ctgctggcga accgggacgc ggaccggctg cggcggtacc
      961 aggagtacct cgattactac gaggggcggc gggggtcgcc gccggcgcgc ggccgggagc
     1021 ggacgctgac gttcaactac gcgcgggcga tggtggagaa gggcgcatcc tacctcgtca
     1081 cggagcaccg gccgcaggtc gtggccgtcg aggacacggc ggccgggcgg cggcaggggg
     1141 cggaggccga gcgcgcgctg gtagaagcgt gggactcgaa cgacctcgcg cggctcgacc
     1201 tcgagaccga ggtggacacg gcggtgctcg gggacggcgc gttcaaggtc gtgtgggacg
     1261 agcgcgagcg gcgggtggtc gtctccgcgc cggacgtgca ggggctgtac gcgtggtggg
     1321 taggcgatga tgtgcggcgg ctgtggcggg tggcggcgcg gtaccggatg ccctcggagc
     1381 aggtggcgga gcggatcggc cacacgccgc gaagcccggg cggccggctg ccggcgacgg
     1441 tggacgtgat cgagtcgtgg acctcggagc gctttgagct gtggctgcaa gggcagccgg
     1501 tcgaggcgag gccgaatccg tatggcgaga tcccgtacgt gatctatcca aacgtgccgt
     1561 ggccgaagca gttctggggc atctcggaca tcgagccgtt gcgcgagccg ctggcggagt
     1621 tgaaccgcgc gctgacacag ctgagccgca ttctcgagct gtcgggcaat ccgatcgcgg
     1681 tgctcgaaaa cgtcgaggag gcgcgagaca tcgccgtgca gccgggggcg gtgtgggaga
     1741 tcccggagca agcgcgggcg tacctcctcg acctgctgca gggcggcggg gtgcggctgc
     1801 acgtggacta cgtggagct
//
LOCUS       DAORPG010000440         1810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8451_length_1810_cov_3.380057, whole genome shotgun sequence.
ACCESSION   DAORPG010000440 DAORPG010000000
VERSION     DAORPG010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8451_length_1810_cov_3.380057"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..1261)
                     /locus_tag="PLX85_08925"
     CDS             complement(227..1261)
                     /locus_tag="PLX85_08925"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC63326.1"
                     /translation="MRRSLALFALLVALLAAFALVGCGDDDRAPEADGTPASRHVTFM
                     AGFRAQANLPFVAVYVAQAKGYFADEGLEVDIRHSSGQDEHLKLLLAKEVDFTTGTAA
                     QLLARRADGLPIRAVALFGQRGDQGFVVRADSGIQRPADFAGKTVGFKAGVVPAELKA
                     LLASGGLSEKDVKLQGVGFDPRVFIEGGLDVYPVFLSNEPYAIRKAGVDVRVFDPADY
                     GVATLGLTFLAHEDTVRGDPDLVRRFLRASMRGVEYAAAHVDEAVDITLRYANGADPG
                     QQRYLLETDLANARRADGIGRSSPEQWDALQATLLKFGALDHATDARAAIDTSIIDGL
                     YGADGRLRPR"
     gene            complement(1282..>1810)
                     /locus_tag="PLX85_08930"
     CDS             complement(1282..>1810)
                     /locus_tag="PLX85_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRC63327.1"
                     /translation="AVVMAHSRPLERALYPLALVVKVTPVVAVAPLFIIWFGFGIWPK
                     LLVAALITFFPMLVNAVTGLRDVDPAALDFLRSLHASRWQVFWRLRGPASLPYLFAAL
                     RISVPLALIGAVVAEWMSGDGGLGQVILIANGDFDTASLFAAVLVLAALAVLLSGALA
                     LLERTVLFWHESFEG"
BASE COUNT          303 a          631 c          648 g          228 t
ORIGIN      
        1 gacggcccag gcgaccgcga gcagcagtcc gggtagcggc agccaccacg ggaaggaggg
       61 ctgcccgtcc tcgagcatgg ccgaacgcag agtgccgtcc agcgtctcat caggctcggc
      121 ctcgcggccg tggctctcgt ctgctgccat gcttcccccg cgcggccgtg catggccatc
      181 gtacccgagc gcctcgacgc ccgcgaacca cgacggccga ggcgggtcag cgcgggcgga
      241 gccggccgtc cgcaccgtag aggccgtcga tgatgctcgt gtcgatggcg gccctcgcgt
      301 cagtcgcgtg gtcgagcgcg ccgaacttga gcagcgtggc ctgtagcgca tcccactgct
      361 ccgggctcga acggccgatg ccgtccgcgc ggcgcgcgtt cgcgaggtcg gtctcgagca
      421 ggtagcgctg ctggccgggg tcggcgccgt tcgcgtagcg cagcgtgatg tctacggcct
      481 cgtcgacgtg cgccgccgcg tattcgacgc cacgcatcga tgcgcgcagg aagcggcgca
      541 cgaggtcggg gtcgccacgc acggtgtcct cgtgcgcgag gaaggtcagc ccgagcgtcg
      601 cgacgccgta gtcggcgggg tcgaagacgc ggacgtcgac gccggccttc cggatggcgt
      661 acggctcgtt gctcaggaac acggggtaga cgtccagccc gccctcgatg aagacgcgcg
      721 ggtcgaaccc cacgccctgc agcttcacgt ccttctcgct cagcccgccg ctcgccagca
      781 gcgccttgag ctcggcgggc acgacgccag ccttgaagcc gaccgtcttg ccggcgaagt
      841 cggccggccg ctggatgccc gagtcagcgc ggacgacgaa gccctggtcg ccgcgttgcc
      901 cgaacagcgc caccgcgcgg attggcaggc catcggcgcg acgggcgagg agctgcgcgg
      961 cggtcccggt cgtgaagtcg acctccttcg ccagcagcag cttgaggtgc tcgtcctgcc
     1021 cgctcgaatg ccggatatcg acctcgaggc cctcgtcggc gaagtagccc ttcgcctggg
     1081 cgacgtagac ggcgacgaag ggcaggttcg cctgcgcccg gaagcccgcc atgaaggtga
     1141 cgtgccgcga cgcgggcgtg ccgtcagcct cgggcgcccg gtcatcgtcg ccgcatccga
     1201 cgagcgcgaa tgccgcgagc agcgccacca gcagggcgaa aagcgccaaa gagcgtcgca
     1261 ttgcacggcc ttccgggagc gtcatccttc gaacgactca tgccagaaga gcaccgtacg
     1321 ttcgagcagg gcgagtgcgc cgctgaggag gacggcgagg gcggcgagca cgagcactgc
     1381 ggcgaacagc gaggcggtat cgaagtcacc gttggcgatc aggatcacct gccccagccc
     1441 gccgtcgcca gacatccact cggccacaac tgccccgatc agggcgagcg gcaccgaaat
     1501 gcgcagggcc gcgaacaggt agggcagcga cgctggcccg cgcagacgcc agaagacctg
     1561 ccacctcgag gcgtggagcg aacgcaggaa atcgagcgcg gcggggtcga cgtcgcgcag
     1621 gccggtgacg gcgttcacca gcatcgggaa gaaggtgatg agcgcggcga cgagcagctt
     1681 tggccagatg ccgaagccga accagatgat gaacagcggc gcgaccgcga cgaccggcgt
     1741 caccttcacc accagcgcca gcggatagag cgcacgctcg agggggcgcg aatgcgccat
     1801 caccaccgcg
//
LOCUS       DAORPG010000441         1807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8477_length_1807_cov_3.483447, whole genome shotgun sequence.
ACCESSION   DAORPG010000441 DAORPG010000000
VERSION     DAORPG010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8477_length_1807_cov_3.483447"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1508
                     /locus_tag="PLX85_08935"
     CDS             <1..1508
                     /locus_tag="PLX85_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRC63328.1"
                     /translation="ATDLHHRGQAQPWLRAAPAQLRLVNARHPLLQGDVVPITLEVGG
                     EVQALLITGPNTGGKTVALKTAGLLCLMALAGLPVPADDGSQVPVYEAVFADIGDEQS
                     IAQSLSTFSGHVTNIIDIIERADDQSLVLLDELGAGTDPTEGAALAIAIVERLRRERV
                     SLIATTHHSELKLYAHHTPGVMNASVEFNVETLAPTYRLTLGLPGQSNALAIAARLGM
                     PADVIEEARAGLSSDQQAMEHVLADLRRQLTAAEERASAAAAARDAAEALRRDYETRL
                     EALTNETADLRAEARRKVRAELRETERLVQRTRRQVEAARLEQASAELTRARRAMEAL
                     PPEPEPEPAPPPMSGPIAIVPGHLVWVRGIPGPGEVLSAPGESDEFEVQLGALRTRVR
                     VQQVERTSPGAPAPAPRMPAPPPPANVANEIEVRGQRLDEAMPRVEEFLDLAARAGKH
                     RVYLIHGKGTGTLRRAVRAMLDRHPLVTSYETAARQEGGEGVTVAYLAGVQ"
     gene            complement(1513..>1807)
                     /locus_tag="PLX85_08940"
     CDS             complement(1513..>1807)
                     /locus_tag="PLX85_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRC63329.1"
                     /translation="DWGVGQVPGEEGMEAFRLRVRAAFDALHERHRESLAVAVVHGGV
                     LGAILGSLSGLAPHEHVQVYSSNCGMTTIADERGQPTVIGLNDVCHLREGAVR"
BASE COUNT          279 a          665 c          611 g          252 t
ORIGIN      
        1 aggccaccga cctgcaccac cgcggccagg cgcagccgtg gctccgcgcg gcccccgcgc
       61 agcttcgcct ggtgaacgcg cgacatccgt tgctgcaagg cgacgtcgtc ccgatcacgc
      121 tcgaggtcgg cggcgaggtg caggcgctac tgatcaccgg cccgaacacc ggcggcaaga
      181 ccgtcgcgct gaagaccgcc ggcctgctct gcctcatggc gctggcgggg ctgcccgtcc
      241 ccgccgacga tgggtctcag gtgccggtgt atgaggcggt gttcgcggat atcggcgatg
      301 agcagtcgat cgcccagtcg ctctccacgt tctccggcca cgtcacgaac atcatcgaca
      361 tcatcgagcg tgccgatgac cagtcgctcg tgctcctcga tgagctcggg gccggcacag
      421 acccgaccga gggggccgcg ctcgcgattg cgatcgtcga gcgtctgcga cgcgagcggg
      481 tctcgctgat tgcgaccacg caccacagcg agctgaagct gtacgcccat cacacacccg
      541 gggtgatgaa cgcgtccgtc gagttcaacg tcgagacact ggcgcccacc taccggctca
      601 ctctgggcct gcccggccag tcaaacgcgc tcgccattgc cgcccgtctc ggcatgccgg
      661 cggacgtgat cgaggaggca cgcgccggcc tctcgagcga ccagcaggcg atggagcatg
      721 tcctcgctga cctccgccgc cagctcaccg ccgccgagga gcgcgccagc gccgcggccg
      781 cagcccggga cgccgccgag gcgctacgtc gcgattacga gacgcgcctc gaggcgctca
      841 cgaacgagac ggccgacctg cgagcggaag cccgacggaa agtgcgcgcc gagcttcgcg
      901 agacggagcg gcttgtgcaa cggacgcgcc ggcaggttga ggccgctcgt ctcgagcagg
      961 cgagtgccga gctcacgcgc gcccgccgcg cgatggaagc cctcccgccg gagccagagc
     1021 ccgagcccgc gccgcctccg atgtccggcc cgatcgcaat cgtgcccggt cacctggtct
     1081 gggtgcgtgg gattcctgga ccaggcgagg tgctctcggc gcccggtgaa agcgatgagt
     1141 ttgaggtgca gctcggcgca ttgcgcaccc gcgtccgcgt gcagcaggtc gagcggactt
     1201 ctccgggcgc acccgcgccg gcgccccgta tgccggcccc gccgcctccc gcgaacgttg
     1261 cgaacgagat cgaggtgcga ggacaacgcc tcgatgaagc gatgcctcgc gtcgaggagt
     1321 tcctcgacct cgcggcccgt gccggcaagc accgcgtcta tctcatccac ggcaaaggga
     1381 cggggacgtt gcgccgcgcg gtacgcgcca tgctcgaccg ccaccctctg gtcacctcgt
     1441 acgagaccgc tgcgcggcaa gaaggtggcg agggcgtgac ggtcgcctac ctcgcggggg
     1501 tgcaatgagc ggctaccgca ccgctccctc gcgcaggtgg cagacgtcgt tgagcccgat
     1561 cacggtcggc tgtccgcgct cgtccgcgat cgtcgtcatg ccgcagttcg agctgtagac
     1621 ctgcacgtgc tcgtgtggcg cgagcccgga caacgacccg aggatcgccc cgaggacgcc
     1681 gccgtgcacc actgcaacgg caagcgattc gcggtgccgc tcatggagcg cgtcgaaagc
     1741 cgcccggacg cgcagccgga atgcctccat gccctcttct ccgggcactt gcccgacgcc
     1801 ccagtcg
//
LOCUS       DAORPG010000442         1807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8481_length_1807_cov_2.589612, whole genome shotgun sequence.
ACCESSION   DAORPG010000442 DAORPG010000000
VERSION     DAORPG010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8481_length_1807_cov_2.589612"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PLX85_08945"
     CDS             complement(<1..823)
                     /locus_tag="PLX85_08945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC63330.1"
                     /translation="MASLVVEGLYRSFSDEVLAVENVSFEVPDGHIAALIGPSGAGKT
                     SVLRMIAGLDEPNLGDVRLDGHSLRRVKPHRRGIGMMFQELALFPHMNVEDNVMFGLR
                     MAKWPKPARRERAEEMLALVGLTAFATRRVDELSGGERQRVALARALAPQPGLLLLDE
                     PLGAIDEERKRWLRNELRAVLRSVQTTALIVSHDIRDAVAIADDVVVMDAGRVLQSGP
                     LATVVAYPITAEAAAMFGYVTLIAGEVRERGVFEQGVGSLPLPPELRLTGRVRVMA"
     gene            complement(859..>1807)
                     /locus_tag="PLX85_08950"
     CDS             complement(859..>1807)
                     /locus_tag="PLX85_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRC63331.1"
                     /translation="VALPAAALVSTIQIGATITALTLYSYFQRGATRSLTLRGERSRR
                     LREAGWGQRLLAFLVVVALGVFIVAPMAALVHGAFTVGASDVATLENFTSLFDDTGRQ
                     SFIPPLDAVRWSLTFAAGSAALATVVGLASARAIAYSRGAFGALGDGLLMLPLTVPAV
                     VLGFGYLVTFNRDPYDLRGSLWLVFAAHALVAYPFVVRSVLSTLRAVDPRVPEAARML
                     GASSWTVWWSVEFPILRRPLLAGALFAFAVSLGEFGATVLLQRREFATLPIAIFDALS
                     KPGEANLGHALAMSTLLMAVTAAAFLLIERLRYRDTTGF"
BASE COUNT          322 a          717 c          536 g          232 t
ORIGIN      
        1 gcgccatgac ccgcacgcgc ccggtcagcc gcagctccgg aggcagcggc agcgacccca
       61 cgccctgctc gaacaccccg cgttcgcgca cctcgcccgc gatcagcgtc acgtagccga
      121 acatcgcggc cgcttctgcc gtgatcgggt acgcgacgac cgtcgcgagc ggtccgctct
      181 gcagcactcg ccccgcgtcc atcaccacca cgtcgtccgc gatcgccacc gcgtcgcgaa
      241 tgtcatggct cacgatgagt gccgtcgtct gcactgaccg cagcacggcc cgcagttcgt
      301 tacgcagcca gcgcttgcgc tcttcgtcga tcgcccccag cggttcgtcg aggagcagca
      361 gccccggctg tggcgccagc gcccgcgcca gcgccacccg ctgccgttcg ccgccggaca
      421 gctcgtcgac gcgccgtgtg gcaaatgccg tcagccccac cagcgccagc atctcctcgg
      481 cccgctcacg cctcgccggc ttcggccact tcgccatccg cagcccgaac atcacgttgt
      541 cttcaacgtt catgtgcgga aagagggcca gctcctggaa catcatcccg atgccccgcc
      601 ggtgcggctt cactcgccgc aacgagtgcc cgtccagccg cacgtcgccc aggttcggct
      661 cgtcaaggcc ggcgatcatg cgcagcaccg aggtcttgcc agcccctgac ggcccgatca
      721 gggccgcgat gtgcccgtcc ggcacctcga acgacacgtt ctcgacggcg agcacctcat
      781 ccgagaacga gcggtacagc ccttcgacga cgagagacgc catgacacac cctggacacg
      841 tgcaatcgcc ggcggagact agaagccggt cgtgtcacgg tagcgtaatc gctcgatcag
      901 taagaaggca gccgccgtca ccgccatcaa caacgtcgac atcgccagcg catgtccgag
      961 gttcgcctcg cccggcttgc tcagcgcgtc gaagatcgcg atcggcagcg tcgcgaactc
     1021 gcgccgctgc agcagcaccg tcgccccgaa ctcgccgagc gacaccgcga acgcaaacag
     1081 cgccccggcg agcagcggcc gtcgcaggat cgggaactcg acgctccacc acactgtcca
     1141 cgaggaggcc ccgagcatcc tcgcggcctc cggcacacgt gggtcgaccg ctcgcagcgt
     1201 cgatagcacc gaacgcacca cgaacgggta ggcgaccagc gcatgcgccg cgaacaccag
     1261 ccacagcgac cctcgcaggt cgtacgggtc ccggttgaat gtcacgaggt agccgaaccc
     1321 cagcaccacc gccggcaccg tcagcggcag catcagcagc ccgtccccga gtgcgccgaa
     1381 ggcgccccgc gagtacgcga tcgctcgcgc ggaggccagc ccgaccaccg ttgcgagcgc
     1441 ggctgacccc gccgcgaacg tcagactcca ccgcaccgca tcgagcggcg ggatgaacga
     1501 ctgccgcccg gtgtcgtcaa acaggctggt gaagttttcc agcgtcgcga catcgctcgc
     1561 gcccaccgtg aacgcgccat gcacgagtgc cgccatcggc gcgacgatga ataccccgag
     1621 cgccaccacc acgaggaacg ccagcaaccg ctgaccccag cccgcctccc gcagccgcct
     1681 cgaccgttcg ccgcgtaacg tcagtgagcg tgtcgccccg cgctggaagt acgaatacag
     1741 cgtgagcgcc gtaatcgtcg ccccgatctg gatcgtggac accagcgccg ccgccggcag
     1801 ggccacc
//
LOCUS       DAORPG010000443         1804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8504_length_1804_cov_3.440252, whole genome shotgun sequence.
ACCESSION   DAORPG010000443 DAORPG010000000
VERSION     DAORPG010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8504_length_1804_cov_3.440252"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..808
                     /locus_tag="PLX85_08955"
     CDS             278..808
                     /locus_tag="PLX85_08955"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC63332.1"
                     /translation="MRVDGLGYPAIIVKNMEESIGFYTKTFGMEHLYTEPNRDDPESA
                     QALLHAGGDTYVLLVGPTNPNVKLAEASLGVGSMQYFSLQVPAAWMDRAFYELSSVGA
                     QASEEIRRGYERLVFMEDPNGVLIILTAWTTEPPAGMSRPAVLARAAAIRDAQRQPFI
                     EDAHIRQAISDLALRQ"
     gene            complement(818..1342)
                     /locus_tag="PLX85_08960"
     CDS             complement(818..1342)
                     /locus_tag="PLX85_08960"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRC63333.1"
                     /translation="MPRPTDPLRDEHRELLPHVELLRTAAESLRSADLEGAAFEDAVH
                     QIDEALAFLNGHLIPHATAEEAALYPVVARVMGAPLATRTMSRDHIEVGRLTEELSKL
                     RERFGAGRTDAVTTSELRQALYGLYALVRLHFAKEEEIYLPLLDDHLDATAAAEMFES
                     MEAAAAAAKAAAGT"
     gene            1481..>1804
                     /locus_tag="PLX85_08965"
     CDS             1481..>1804
                     /locus_tag="PLX85_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRC63334.1"
                     /translation="MFTTDQYHGMLAETVTVNGANGDAIHAYLARPLGAGPFPAVVLI
                     HHMPGWDEFYFEMTRKFAHHGYLAISPDLYCRDAHGAPDDVAAKVRADGGAPDARVVE
                     DVAGAA"
BASE COUNT          276 a          559 c          696 g          273 t
ORIGIN      
        1 attaccagga catgatcgtg atggggctcc tcgaagggga gttgcggcct gaggggtgag
       61 taccggcggg acggctgaca gcgttgcgcc aagctcgtcg gtggacgagc gggaagccgg
      121 gcattaccag gacatgatcg tgatggggct cctcgaaggg gagctgcggc gcgaatgagc
      181 ggtgtggggg ccgcgcccgc gtaggtggtg caggccgtac gctggcgagg cgcgggcctc
      241 gaggggggcg cggggtacct cggaaggtgg ggacgagatg cgggtggacg gcctcgggta
      301 cccggcgatc atcgtgaaga acatggagga gtcgatcggc ttctacacga agacgttcgg
      361 catggagcac ctctacaccg agccaaaccg cgacgacccc gagtccgccc aggcgttgct
      421 gcacgcgggc ggcgatacgt acgtgctgct ggtcgggccg acgaacccga acgtcaagct
      481 ggccgaggcg tcactcggcg tagggtcgat gcagtacttc agcctgcagg tgccggcggc
      541 gtggatggac cgcgccttct acgagctgtc ctcggtgggg gcgcaggcga gcgaggagat
      601 ccgccgcggc tatgagcgtc tggtgttcat ggaggacccc aacggggtgc tgatcatcct
      661 caccgcgtgg accaccgagc cgcccgcagg gatgtcgcgg ccggcggtgc tggcgcgagc
      721 cgccgcgatc cgggacgcgc agcggcagcc gttcatcgag gatgcacaca tccgccaggc
      781 gatctcggac ctcgcgctgc ggcagtaggc ccgcggctca ggttccggca gccgccttcg
      841 ccgcggcggc ggcggcttcc atcgattcga acatttcggc ggcggctgtc gcgtcgaggt
      901 ggtcgtcgag cagcggcagg tagatctcct cttccttggc gaagtgcagg cgcacgagag
      961 cgtagaggcc gtagagggcc tgcctcagct cgctggtcgt gaccgcgtcg gtgcggccgg
     1021 cgccgaaacg ctcacggagc ttcgagagtt cctcggtgag gcgaccgacc tcgatgtggt
     1081 cgcggctcat ggtgcgcgtc gcgaggggcg cgcccatgac gcgcgcgacg acggggtaga
     1141 gcgcggcctc ctcggcggtc gcgtgcggga tgagatggcc gttgaggaac gcgagtgcct
     1201 cgtcgatctg atgcacggcg tcctcgaagg ccgcgccctc gaggtcggca cttctgagcg
     1261 actcggcggc ggtgcgcagc agctcgacgt gagggagcag ctcacggtgt tcgtcgcgca
     1321 gggggtcagt gggtcggggc attgggggtc tcctcgagct cggtcgcgcg gtgcctccgc
     1381 gggcggccac agcagtgtag aaggacgtgc cgacagcgcg atgcggcgcg gtgcaccggc
     1441 gtggtacaac cctcgttgcc tgcgcacgaa gggggattcg atgttcacca ccgaccagta
     1501 ccacggcatg ctcgcggaga ccgtgaccgt caacggcgcg aacggggacg cgatccacgc
     1561 gtatctcgcg aggccgctcg gggccgggcc gtttccggcg gtcgtgctga tccaccacat
     1621 gcccggctgg gacgagttct acttcgagat gacgcgcaag ttcgcccatc acggctacct
     1681 ggcaatcagc cccgacctct actgccgcga cgcgcacggc gcgccagacg acgtggccgc
     1741 aaaggtgcgc gccgatggcg gcgcgcccga cgcgcgcgtg gttgaggacg tggcgggcgc
     1801 cgcg
//
LOCUS       DAORPG010000444         1796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8569_length_1796_cov_2.709362, whole genome shotgun sequence.
ACCESSION   DAORPG010000444 DAORPG010000000
VERSION     DAORPG010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8569_length_1796_cov_2.709362"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PLX85_08970"
     CDS             complement(<1..448)
                     /locus_tag="PLX85_08970"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HRC63335.1"
                     /translation="MLHESIRLLEHREFRNPIVIAGFASQGGVTPAAVIQHLAEHWDA
                     TAFAEIDPEEYFDFTVRRPFVQIDGEERILQWPVTRLYAASIDGLDRDVVLLSGIEPS
                     LRWRMFTDAIASLLAEIGATTFVTVTAYPGAVPHTRPLPVRLDAADS"
     gene            complement(473..928)
                     /locus_tag="PLX85_08975"
     CDS             complement(473..928)
                     /locus_tag="PLX85_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63336.1"
                     /translation="MTSIVRDVREPVVSAGAANLTTIRRPASLLAGRFYSLLALPDDQ
                     LAGGMTRYIHAEHDLSEPARYEAVLARLQAWLELDVEDRIVIGRAWERALDGLPLEYR
                     ARCREAERAVMMNGLTFGDFRALAAALPWLRQRDYLGASLEPSTALTGV"
     gene            1161..1613
                     /locus_tag="PLX85_08980"
     CDS             1161..1613
                     /locus_tag="PLX85_08980"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRC63337.1"
                     /translation="MTDASTIGEVAQRTGLTVSAIRYYEAEGIVAPASRTEAGYRLFT
                     ETDVRRLLLAKQARHLGLGLREVKALVEDLATLDCATFAGELGSRIAGQRAAVAARIA
                     ELRELEATLEALETHIDSCVCRPGTAAADCAITLFDHEREDHDDCSCM"
BASE COUNT          296 a          625 c          596 g          279 t
ORIGIN      
        1 cagagtccgc ggcatcgaga cggacgggca gtggccgggt gtgcggcacc gcgcctggat
       61 acgccgtcac ggtcacgaac gtcgttgcgc cgatctccgc cagtagcgag gcgatggcgt
      121 cggtgaacat gcgccagcgg aggctcggtt cgatgcccga cagcagcacg acgtcgcggt
      181 cgagcccgtc gatggacgcc gcatacaggc gggtaacggg ccactgcagg atgcgctcct
      241 cgccgtcgat ctgaacgaac ggacggcgaa cggtgaagtc gaagtactcc tcagggtcga
      301 tctcagcgaa cgcggtcgca tcccagtgct cggccaggtg ctggatcact gccgccggcg
      361 tgacgccgcc ctggctcgcg aagccggcga tcacgatcgg gttgcgaaac tcacggtgct
      421 cgagcaggcg aatcgattcg tgcagcatgg cgaactcctc tcgaaccggc ggctagacgc
      481 ccgtgagggc ggtcgacggc tccagggatg cgcccaggta gtcgcgctgt cgtagccagg
      541 gcagagcagc ggccagcgca cggaagtccc cgaatgtcag cccgttcatc atcacggcgc
      601 gctccgcttc ccggcaccgc gcccggtact ccagcggcag gccgtcgagc gcgcgctccc
      661 acgcgcgccc gattacgatc cggtcctcga cgtcgagttc gagccacgcc tgcaggcggg
      721 caagtaccgc ctcgtagcgg gccggttcgg acagatcgtg ctccgcgtgg atgtacctcg
      781 tcatcccgcc ggcgagctgg tcgtccggca gtgcgaggag ggagtagaac cgccccgcga
      841 ggagcgaagc cggccgccgg atggtcgtga ggttcgccgc cccggcgctc accacaggtt
      901 cgcggacgtc gcgaacgatc gaggtcacca ttgctgacac cctcctgtat tcgtgatgcc
      961 ttgtcctcga tgacgagcga ggatgctccc cgcgtgaaca tatcttagca gtcgcctcaa
     1021 gggactgcta ataactgagc atgtactgag ctggtcggct ccgaccgttc ctgccaacgc
     1081 tgctccggtt ccatcgccgc cgtgacatgc ttgaccctca agccgcttga ggtcctacgg
     1141 ttcgatgcgg aggcagggac atgaccgacg cgagcacgat tggcgaggtg gcacagcgca
     1201 cgggactcac cgtctcggcc atccgctact acgaggccga aggcatcgtg gctcccgctt
     1261 cgcgcaccga ggccggctac cgcctcttca ccgagaccga cgttcgccgg ctcctgctcg
     1321 cgaagcaggc gcgccacctc ggactcggcc tccgcgaggt caaggccctc gtcgaggacc
     1381 tcgcgacgct cgactgcgcg accttcgccg gcgagctcgg cagccgtatc gcgggtcagc
     1441 gggcggcggt tgccgcgcgc atcgccgagc tccgcgagct cgaagcgact ctcgaggcac
     1501 tcgagaccca tatcgacagc tgcgtctgcc ggcccgggac cgcggccgca gactgtgcaa
     1561 tcacgctctt cgaccacgag agggaggacc acgatgactg ttcgtgcatg tgactgcggc
     1621 gccggctgcg actgctcctg cggcggctgc tgctagcggc tcggacgcga cgaggggcgg
     1681 caatcgccgc ccctcgcatc gcacaccgat aagcggaccg ctaccccgcg agcaccccgc
     1741 acttcacgag gtgcgccccc agcaggcggt tgaactcctc ggtcgcttcg atgttc
//
LOCUS       DAORPG010000445         1795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8576_length_1795_cov_2.951724, whole genome shotgun sequence.
ACCESSION   DAORPG010000445 DAORPG010000000
VERSION     DAORPG010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8576_length_1795_cov_2.951724"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            427..1521
                     /gene="fbp"
                     /locus_tag="PLX85_08985"
     CDS             427..1521
                     /gene="fbp"
                     /locus_tag="PLX85_08985"
                     /EC_number="3.1.3.11"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphate aldolase/phosphatase"
                     /protein_id="HRC63338.1"
                     /translation="MPQTLSVIKADVGGFVGHSAMHPEVLDQGREFLARAVQSGLLID
                     GHASSCGDDMFLIMSHDRGENNEEIHKLAWDAFIAGTDVAKKLHLYGAGQDLLVDAFS
                     GNIRGAGPGSAELELEERPSEPVIVFMGDKTSAGSFNLPFYKMFADPFNTAGLVISEP
                     LHSGFAFEVHDVKEHRKIVLNTPEEMYDLLVFIGSPARYAVKRVVSRLTGEVGAVSST
                     DKLSLIAGRYVGKDDPTAIVRTQGNFPAVGEVLEPFTTAWTVEGFMRGSHYGPWMPTS
                     VKQATPARFDGPPRVVALGFQLAEGKLIGPRDMFDDPSYDNARAEANRVADYLRKHGP
                     FEPHRLPLDEMEYTTMPAVAAKLSSRWDEI"
     gene            1529..>1795
                     /locus_tag="PLX85_08990"
     CDS             1529..>1795
                     /locus_tag="PLX85_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HRC63339.1"
                     /translation="MSTLPRLLLASSNAGKAREIGLLLGNSGWRVVTPAEAGVGPVEV
                     IELGRSYLENATQKAVAYCEASGMPALADDSGLEVDVLGGRPGVR"
BASE COUNT          265 a          683 c          578 g          269 t
ORIGIN      
        1 gcaagctgca gggcttccgc gccctcgacg aggatcatgc gcgccgccgc ggcctcgaga
       61 tgctggccgc gctgcgcgcc ttcctcgcca gcccggcgga cctcgactgg aacctgcagg
      121 cgcgcacgcg ccccctcgcg caggccgccg tcgagcggct ccaggcccgc ttcgagtagt
      181 ccgttgcccc gccgcccgat cgcggtttgc cgggcccagt gcgtttgata cgatgcccct
      241 gcctcccgcc gtgcaacgag cctccgcgtg gcagcccttg ccacgaccgg tacggtggcg
      301 ctgcaggccc gtggcggctc gtcattgccc actgcctcgc gagaggcacg tcattcaggc
      361 ccgcgcccga ggtccgagcc atcggccggg agcgagcggt gcagaggatt acagggagga
      421 gcggccatgc cgcagacgct gagcgtgatc aaggcggacg tcggaggctt cgtcgggcac
      481 tccgcgatgc atcccgaggt gctcgaccag ggccgtgagt tcctcgcgcg cgccgtccag
      541 tccggcctgc tgatcgacgg tcacgcttcc tcctgcggcg acgacatgtt cctcatcatg
      601 tcccacgacc gcggcgagaa taacgaggag atccacaagc tcgcctggga cgccttcatc
      661 gccggcaccg acgtggcgaa gaagctccac ctctatggcg ccggccagga cctgctggtc
      721 gacgcgttct ccggcaacat ccgcggcgcc ggtcccggct ccgccgaact cgagctggag
      781 gagcgaccgt ccgagcccgt gatcgtgttc atgggcgaca agacctcggc gggctccttc
      841 aacctcccct tctacaagat gttcgccgac cccttcaaca cggccggcct cgtcatctcc
      901 gagccgctgc actccggctt cgcgttcgag gtgcacgacg tcaaagagca ccgcaagatc
      961 gtcctcaaca cccccgagga gatgtacgac ctgctggtct tcatcggctc gcccgcccgc
     1021 tacgcggtga agcgcgtcgt gagccgcctg accggcgagg tcggcgccgt ctcctccacg
     1081 gacaagctct cgctcatcgc cggccgctac gtcggcaagg acgaccccac cgccatcgtg
     1141 cgcacgcagg gcaacttccc cgcggtcggc gaggtgctcg agcccttcac gacggcctgg
     1201 accgtcgagg gcttcatgcg cggctcgcac tacggcccct ggatgcccac ctccgtgaag
     1261 caggccacgc cggcccgctt cgacggcccg ccgcgcgtgg tcgcgctcgg cttccagctc
     1321 gcggagggca agctcattgg cccgcgcgac atgttcgacg acccgtccta cgacaacgcc
     1381 cgcgccgagg ccaaccgggt cgccgactac ctgcgcaagc acggcccctt cgagccgcac
     1441 cgcctgccgc tcgacgagat ggagtacacg acgatgcccg ccgtcgccgc caagctctca
     1501 agccgctggg acgagatcta gcagcggctt gagcacgctg cctcggctcc tgctcgcttc
     1561 ctcgaacgcc ggcaaggcgc gcgagatcgg cctgctgctc ggcaacagcg gctggcgcgt
     1621 cgttaccccc gccgaggccg gcgtcggccc ggtcgaggtg atcgagctcg gccgctcgta
     1681 tctcgagaac gcgacccaga aggcggtcgc gtattgcgag gcgagcggca tgccggcgct
     1741 cgcggacgac tccggcctcg aagtggacgt cctcggcgga cgccctggcg tccgt
//
LOCUS       DAORPG010000446         1790 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8615_length_1790_cov_2.972911, whole genome shotgun sequence.
ACCESSION   DAORPG010000446 DAORPG010000000
VERSION     DAORPG010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8615_length_1790_cov_2.972911"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1072)
                     /locus_tag="PLX85_08995"
     CDS             complement(116..1072)
                     /locus_tag="PLX85_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRC63340.1"
                     /translation="MSHEVPPYTSTRLTAQLGSILTVMLALLFASGALFFLFIFNYDL
                     PPWFTWVRFFHFYTGLASIPILVGKYGSTLVRFGGYYLRVPRYKAAGPPSLVPRVLSP
                     LLMADFFVLYFSGLYMLFHRYYTVTNIPPFDAKPVQVHLWAAIVGAPLVTVHLVYHAA
                     SAMRSVRGRSPASATEVARYAETSSGTLTRRALLGGMAGTGLGLALLFQNTRARSVEF
                     ATLFIGRIPEEERGGPGDFPVETLFGKEAVPDVGAYRLEVTGEVGRPLMLSYQELLAL
                     PSYEHRIRLSCVSGWTQRVVWRGPRVRDVLRPHEEGPWQRPT"
     gene            complement(1069..>1790)
                     /locus_tag="PLX85_09000"
     CDS             complement(1069..>1790)
                     /locus_tag="PLX85_09000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HRC63341.1"
                     /translation="LTPPERLATALDVLHSEPRRCLVVLGRTPEMGRGKTRLAATLGD
                     QATYELARAFFDDTCGLAAAGERGGSWRSLLLYEGRAPVGRAIEATPQPLGDLGTRIR
                     AGLEAGLKRGERVVLIGTDTPDVARAYLDGAFEALEEADVVIGPARDGGFTLIGAQDT
                     LSLDPSIFDGVEWSTSTVFEQTVANLDRLGLRVAVLDEREDVDDIGSLGRLARRLDGE
                     AAEVAPRTRAALAAVFAEVRA"
BASE COUNT          293 a          666 c          583 g          248 t
ORIGIN      
        1 aagccgatca ccatcgcgat ccagacgagc agcaggatga actgctggtt gccgaacagc
       61 gcgcgcagcc ggctcgtcgg agcgacgacc tgggtgacag gtgactccgg cggcttcacg
      121 tcggtcgttg ccacgggccc tcctcgtggg ggcggagcac gtcgcgcacg cgggggccgc
      181 gccagacgac gcgctgcgtc cagccgctga cgcagctcag ccggatgcga tgctcgtagc
      241 tcggcagcgc gaggagttct tggtagctca acatgagcgg acggccgacc tcgccggtca
      301 cctcgaggcg atacgcgccg acgtcgggca ccgcctcctt gccgaacagc gtttcgacgg
      361 ggaagtcgcc cggccccccg cgctcttctt cggggatgcg cccgatgaac agggtcgcga
      421 actccacgga ccgcgcgcgg gtgttctgga agagcaacgc cagcccgagg cccgtccccg
      481 ccatcccgcc cagcagcgcc cgccgcgtca gcgtcccgct cgaggtctct gcatacctcg
      541 cgacctccgt cgcggacgcg gggctgcgcc cacgcaccga ccgcatcgca ctcgcggcgt
      601 ggtacacgag gtgcaccgtc acgagtggcg cgccgacgat cgccgcccat aggtgcacct
      661 gcacgggttt cgcgtcgaac ggcgggatgt tcgtcacggt gtagtagcgg tggaacagca
      721 tgtacagccc gctgaagtac agcacgaaga agtctgccat cagcagcgga gacagcactc
      781 gagggacgag cgagggcggc ccggccgcct tgtagcgtgg cactcgcagg tagtaccccc
      841 cgaaccgcac cagcgtgctg ccgtatttcc cgaccaggat tgggatcgac gcgaggcccg
      901 tatagaagtg gaagaaccgc acccatgtga accacggcgg caggtcgtag ttgaagatga
      961 acaggaagaa cagcgccccg ctcgcgaaca gcagcgcgag catcaccgtc aggatcgacc
     1021 ccagctgcgc cgtgagccgc gtgctcgtgt acggcggtac ctcgtggctc acgcccgcac
     1081 ctccgcgaac accgcagcca gcgccgcccg cgttcgaggc gcgacctcgg ccgcctcccc
     1141 gtcgagtcgc cgcgccagcc gccccagcga cccgatgtcg tcaacgtcct cgcgctcatc
     1201 gagcaccgcg acgcgcagtc cgagccgatc gaggttcgcc accgtctgct caaacaccgt
     1261 cgaggtgctc cactcgacac cgtcgaatat cgacgggtcg aggctcaggg tgtcctgcgc
     1321 accgatcagc gtgaatccac cgtcgcgtgc cggcccgatc accacgtcgg cctcctcgag
     1381 cgcctcgaac gcgccgtcga ggtacgcgcg cgccacgtca ggtgtgtccg tcccgatcag
     1441 caccacccgc tcgccgcgct tcagtcccgc ctcgaggccg gcccggatgc gcgttccgag
     1501 gtcaccgagg ggttgcggcg tcgcctcgat ggcccggccc acgggtgcgc gaccctcgta
     1561 cagaagcagg cttcgccagc ttccgccgcg ctcgcccgcg gcggcgagcc cgcatgtgtc
     1621 atcgaagaac gcgcgcgcca gctcgtacgt ggcctggtcg ccaagtgtcg ccgccagccg
     1681 cgtcttccct cgccccatct ccggcgtccg cccgagcacg acgaggcagc gcctcggctc
     1741 agagtgcagt acatccagag ccgtcgcgag ccgctcgggc ggcgtcagca
//
LOCUS       DAORPG010000447         1789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8623_length_1789_cov_3.902537, whole genome shotgun sequence.
ACCESSION   DAORPG010000447 DAORPG010000000
VERSION     DAORPG010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8623_length_1789_cov_3.902537"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLX85_09005"
     CDS             <1..228
                     /locus_tag="PLX85_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63342.1"
                     /translation="ESWSRADEVERLLMFREVVRLRPDVPVELPPSSPPDWERGAEAL
                     RALGAEKSAERLLALRNELAAAERSPRLGGL"
     gene            complement(289..645)
                     /gene="rplT"
                     /locus_tag="PLX85_09010"
     CDS             complement(289..645)
                     /gene="rplT"
                     /locus_tag="PLX85_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008852237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRC63343.1"
                     /translation="MARVRSRVAAHARHKKILDLAKGHRGKRRTNYKVAHESVMHALR
                     YATDHRRDKKGDFRRLWIVRINAAARLHDLTYGRLMNGLKKAGIELDRKTLADLAVRE
                     PAAFGRVAEQAKAALG"
     gene            complement(667..864)
                     /locus_tag="PLX85_09015"
     CDS             complement(667..864)
                     /locus_tag="PLX85_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HRC63344.1"
                     /translation="MKTHKGAAKRVKITGSGRVLMMSHHRNNKRMKKSREKLYALRGM
                     EPIHSTHEKRVRRLLPYATIS"
     gene            complement(877..1470)
                     /gene="infC"
                     /locus_tag="PLX85_09020"
     CDS             complement(877..1470)
                     /gene="infC"
                     /locus_tag="PLX85_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005808206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRC63345.1"
                     /translation="MAKELRINERILAPRVRLIREEEQLGIVRTDDALRMAEEAGLDL
                     VEVAPTADPPVCRIMDYGKFKYEQSKREKESRKGSKHSELREVRMRVKIDEHDIDFKA
                     RTARKLLAEGDKVKVSVMFRAREITHPEIGRELLDKFYEKIRDVSTLERNPAMEGRMM
                     SIIVTPGVKRERPPAGAGARSERPAVEAASSAAVGGN"
     gene            complement(1583..>1789)
                     /locus_tag="PLX85_09025"
     CDS             complement(1583..>1789)
                     /locus_tag="PLX85_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HRC63346.1"
                     /translation="VVVDVRVAVCDRFSAGQTLLVIEAMKMQNEIQAPHDGTVKAIHC
                     ERGGRVEQGALVLEYDADGTETAS"
BASE COUNT          245 a          649 c          544 g          351 t
ORIGIN      
        1 gagagctggt cccgcgccga cgaggtggag cgattgctca tgttccgcga ggtcgttcgc
       61 ctccgcccgg acgtgccggt cgaactgcca ccctcgagcc cgcccgactg ggagcgcggc
      121 gccgaggcct tgcgcgcgct cggtgctgag aagtccgctg aacgcctcct cgctctccgt
      181 aacgaactgg ccgccgccga gcgctcgccg cgcctcggcg gactgtagcc ggcaacgaag
      241 atggcggccg agaggccgcc ttcttcgtaa ctcgtccgga ggtgagcgct atccgagcgc
      301 ggccttcgcc tgctccgcta cgcgcccgaa tgctgccggc tctcgcaccg cgaggtcggc
      361 cagcgtcttg cggtccagtt cgatgccggc cttcttcagg ccgttcatca gccgaccgta
      421 ggtgaggtcg tgcagccggg ccgccgcgtt gatgcgcacg atccacagcc ggcggaagtc
      481 gcccttctta tcgcgccggt gatccgtcgc gtaacgcagg gcgtgcatga ccgactcatg
      541 cgcgaccttg tagttcgtcc gccgctttcc tcgatgcccc ttggcgaggt cgaggatctt
      601 cttgtggcgc gcgtgcgcgg caacgcggct ccggactcgg gccatggctc gtgctccctg
      661 gactgtctag ctgatcgtcg cgtacggcag caggcgccgg acgcgcttct cgtgcgtgct
      721 gtggatcggc tccatgcccc gcagcgcgta cagcttttcg cggctcttct tcatccgctt
      781 gttgttgcgg tggtgcgaca tcatcagcac gcggcccgag cccgtgatct tcacgcgctt
      841 cgccgcgccc ttgtgcgtct tcatcttcgg cattgcttag ttgcccccaa ccgccgccga
      901 ggacgccgcc tcgactgcag gtcgttcact gcgcgcacca gcccctgcgg gcggacgctc
      961 tcgcttcacc ccgggcgtca cgatgatcga catcatgcgc ccctccatcg ccggattgcg
     1021 ctccagcgtc gacacgtctc ggatcttctc gtagaacttg tcgagcagtt cgcgcccgat
     1081 ctcggggtgc gtgatctccc gcgcgcggaa catcacggac accttgacct tgtcgccctc
     1141 ggcaagcagc ttccgagcgg tgcgcgcctt gaagtcgatg tcgtgctcgt cgatcttcac
     1201 ccgcatgcgc acctcgcgca actccgagtg cttcgagccc ttccgcgact ccttctcgcg
     1261 cttcgactgc tcgtacttga acttgccgta gtccatgatc cggcagaccg gggggtcggc
     1321 cgtcggcgca acctcgacga ggtcgagacc cgcttcctcc gccatccgca gcgcgtcgtc
     1381 cgtgcggacg atgcccagct gctcttcttc ccggatcaac cggacgcgcg gcgcgaggat
     1441 gcgctcgttg atccgcagtt ccttagcgat agacgtatgc ctccgtgaac gaggtggccc
     1501 cgaccggcgc caccagacga cgattctagc atcgcccgat cagcgctcac aacgagtgca
     1561 tgacccggcg atcaagcctc cgttacgagg ccgtctccgt cccatcggcg tcgtattcga
     1621 ggacgagcgc accctgttcg actcggccgc cgcgttcgca gtggatggcc ttcaccgtgc
     1681 cgtcgtgcgg ggcctggatc tcgttctgca tcttcatcgc ttcgatcacc aacagcgtct
     1741 gaccggcgct gaagcggtcg cagaccgcca ctcgcacgtc aacgaccac
//
LOCUS       DAORPG010000448         1777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8718_length_1777_cov_4.562137, whole genome shotgun sequence.
ACCESSION   DAORPG010000448 DAORPG010000000
VERSION     DAORPG010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8718_length_1777_cov_4.562137"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..1193)
                     /locus_tag="PLX85_09030"
     CDS             complement(213..1193)
                     /locus_tag="PLX85_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015101178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HRC63347.1"
                     /translation="MTEAPVLTRAYRAGSLVAEGFPLADVSSFLESSGTVVWVDLLQP
                     SYDQLITLAHELGLHELAVEDALTRSQRPKLDRYSTHLFFSCQALHLDRQTRNLDRTE
                     IDAFINERWLVTVRKSDGFSLDHFVERIERSPDLLAHGVGYLVYVLLDLVIDGYFDAV
                     EAFDEYYDEASDRIFGETPFTPLQQRDWFQMRQALVRFHRVAFPMREAVNAFLRREHE
                     ISADLHPYYQDVYDHVLRVVESTEALRDLVATIVETNLSLRDYRQNQIMKKVTSWAAI
                     IAVPTLITGYYGMNVPYPGAGETWGVVASSGLALLLSSALYLLFRRNDWL"
     gene            1302..>1777
                     /locus_tag="PLX85_09035"
     CDS             1302..>1777
                     /locus_tag="PLX85_09035"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HRC63348.1"
                     /translation="MTSTSDGQMAAGTRGVRFKPSLPVTLGLLGLAFVVLVSLGVWQL
                     ARQRQADAAEHARDESVAAAPLEWRTEPPYQASEVDFHRVQVSGRWDNAHTMTLANVA
                     RYGTRGEEVVTPLQPDDGGPAILVNRGWYPLTERDRVLAQLASEPRATVEGLARAPL"
BASE COUNT          309 a          518 c          666 g          284 t
ORIGIN      
        1 cgaggccgtt tgggaggtcg agttcggcgc ggacggcgtc ccagctccat tcgggccgaa
       61 gtcgatcgag gtccatcagg ttgggggcca cggtcgcctc gtgaggccgc ttttgaatca
      121 cagcgtggct catgaccatg gcgtggctcc ctcggacgct acgtctgacc tcgaacgggg
      181 cgagtatagc gcgggcctga ggagcgctcg attcagagcc agtcgttcct gcggaagagc
      241 aggtagaggg cgctcgagag caagagggcg agaccggagg aggcgaccac gccccacgtc
      301 tcgcccgcac cggggtacgg cacgttcatc ccgtagtagc cggtgatgag cgtgggcacg
      361 gcgatgatgg cggcccagct cgtgaccttc ttcatgattt gattctggcg gtagtcgcga
      421 agactcagat tcgtctcgac gatggtggcg acgaggtcgc ggagagcctc ggtggattca
      481 acgacgcgga ggacgtggtc gtagacgtcc tggtagtagg ggtgcagatc ggcgctgatc
      541 tcgtgttcgc gtcggaggaa ggcgttgacc gcctcgcgca tggggaaggc gacgcgatgg
      601 aagcgcacga gcgcctggcg catctggaac cagtcgcgct gctggagagg cgtgaagggg
      661 gtttcgccga agatgcggtc gctcgcctcg tcgtagtact cgtcgaacgc ctcgacggcg
      721 tcgaagtagc catcgatgac caggtcgagc aggacgtaga cgaggtatcc gaccccgtga
      781 gccagcagat ccggcgaccg ttcgatgcgc tccacaaagt ggtcgagtga gaagccgtcg
      841 ctcttccgaa cagtgacgag ccagcgctcg ttgatgaacg cgtcgatctc ggttcgatcg
      901 aggttcctcg tctggcgatc gagatggagc gcctggcacg agaagaagag gtgggtcgag
      961 tagcggtcga gcttgggacg ctggctgcgg gtgagggcat cctcgaccgc cagttcatgg
     1021 agtccgagct cgtgcgcgag cgtgatcaac tggtcgtatg acggctggag caggtcgacc
     1081 cagacgactg tccccgaaga ctcgaggaac gacgagacat cagcaagggg aaacccctcc
     1141 gcgacgaggg aaccagcgcg gtacgcgcgc gtgaggaccg gtgcttcggt cacgaggtca
     1201 tgatgcgccg ccggatggcg actgtctcgt gagcgggccg ccatgaggca ccgcggccgt
     1261 ctggagttgc cgtcgtgtgg tgcgaggtgg aacctcgatg gatgacctcg acatcggacg
     1321 gacagatggc ggctgggacg cgcggggttc ggttcaagcc gtcgctcccc gtgacactgg
     1381 ggctgctcgg gctggcattc gtggtcctcg tcagcctcgg ggtatggcag ctggcgcggc
     1441 agcgccaggc ggatgcggcc gagcacgccc gagatgagag cgtggcggcg gcgcccctcg
     1501 aatggcgaac ggagccgcca taccaggcga gcgaggtgga cttccaccgg gtccaggtga
     1561 gcggccggtg ggacaacgcg cacacgatga cactcgcgaa tgtcgcgcgc tacggcacgc
     1621 gcggcgagga ggtcgtcacg ccgctgcagc ccgacgacgg cgggccggcg atcctagtga
     1681 accgcggctg gtacccgctc acggagcggg accgcgtgct cgcgcagctg gcaagcgagc
     1741 cccgtgcgac tgtcgagggg ctggcccgcg cgccgct
//
LOCUS       DAORPG010000449         1775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8740_length_1775_cov_3.256395, whole genome shotgun sequence.
ACCESSION   DAORPG010000449 DAORPG010000000
VERSION     DAORPG010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8740_length_1775_cov_3.256395"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..611)
                     /locus_tag="PLX85_09040"
     CDS             complement(153..611)
                     /locus_tag="PLX85_09040"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRC63349.1"
                     /translation="MTARASQRDPVRDAMSAGGVVYRRSDDRIEVVLVARPSQQLWAL
                     PKGTPEPGESVEATAIREVGEETGLEVRVEREIGSIHYWYAVPEERVRVRKVVHHFLM
                     TATGGDVGRHDHEYDVVEWMDIQEAMARMSYQNERSIVERAEALIRERSA"
     gene            complement(608..1630)
                     /locus_tag="PLX85_09045"
     CDS             complement(608..1630)
                     /locus_tag="PLX85_09045"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRC63350.1"
                     /translation="MPNQDDSSSTNPGGFDDAGAPGDLDEMGGRRRPTAQPRDVLRAI
                     GEGGSLPYDDLPALSAADDQTIAELLPVWPRLAPERRRELLAALQQLSEEDATLDFNR
                     VHLTALHDPDPATRILAIRGLWEHEGEEVMRLLTDLVREDPEGTVRAEAASALGQFVV
                     SMEFGLLSEDGAEHLAETLRDVLEDATQEDEVRGRALEALGASSEEWVAEAIADQYET
                     GTPRMRLASIRAMGRNASDDWLPVLIYNFDDDDAETRAAAATAAGELLLEAAVEPLSM
                     LVDDSDSEVQIAAIEALGEIAGEAAEAALQQLLHREEHIAQAARRALAEARMMAADLD
                     EGSEPA"
BASE COUNT          248 a          652 c          585 g          290 t
ORIGIN      
        1 cgcgcgatac gacatggtga gcggacgcgc cgcgtcgaaa cgggcgagct cggggtcgcg
       61 ccgccattcg tagtcgcggc gagcgtcgtc gagctgcttc tcgcgcagcc gcacgaggcg
      121 gcctgtgcca atgacgcgcg gtgcggtcgt cgtcatgcgc tccgttctcg gatcagcgcc
      181 tccgcgcgtt cgacgatcga gcgctcgttc tggtaggaca tacgggccat cgcctcctgg
      241 atgtccatcc actcgaccac atcatactcg tggtcatgcc gccccacgtc gcccccggtg
      301 gccgtcatca gaaagtggtg caccaccttc cgcacgcgca cccgttcttc cggaacggcg
      361 taccagtagt gaatcgaccc gatctcacgt tcgacccgta cttcgaggcc agtctcctcg
      421 cccacttctc ggatcgcggt tgcctcgacg ctctcacccg gctcgggagt ccccttgggg
      481 agcgcccaga gctgctggct cgggcgcgcg acgaggacga cctcgatgcg gtcgtccgac
      541 cgccggtaga cgacgccgcc tgccgacatc gcgtcacgca cgggatcgcg ttgcgaggcg
      601 cgagcggtca cgctggctcc gaaccctcgt cgaggtcggc cgccatcatc cgcgcctccg
      661 ccagcgcgcg ccgcgccgcc tgcgcgatgt gctcctcgcg atgcaacaac tgctgcaggg
      721 cggcctccgc ggcctcgccg gcgatctctc cgagtgcctc gatcgcggcg atctggacct
      781 cgctgtccga gtcgtcgacg agcatcgaaa gcggctcgac cgccgcttcg aggagcaact
      841 cacccgcggc ggtcgcggcc gccgcgcggg tctcggcgtc atcgtcatcg aagttgtaga
      901 tgagcaccgg caaccagtcg tctgaggcgt tccggcccat ggcccggatc gatgccaggc
      961 gcatgcgagg cgtcccggtc tcgtactggt ccgcaatcgc ctcggcgacc cattcctcgg
     1021 acgacgcgcc gagggcctcg agtgctcggc cccgtacctc gtcctcctgc gtcgcgtcct
     1081 ccaggacgtc ccgcagcgtc tcggcgaggt gctcggcgcc gtcctccgac agcagcccga
     1141 actccatgct gacgacgaac tggccaagtg cgctggctgc ctcggcccgc accgtcccct
     1201 cggggtcctc ccgtacgagg tcggtcaaca gccgcatgac ctcttcaccc tcatgctccc
     1261 agagcccgcg aatcgcgagg atccgtgtcg cggggtccgg atcgtgcagg gcggtgagat
     1321 ggacgcgatt gaagtcgaga gtcgcgtcct cctcggacag ctgctgcagt gcggcgagca
     1381 gttcgcgccg ccgctccggg gccaggcgcg gccacaccgg gagcagttcg gcgatcgtct
     1441 gatcgtccgc cgcggagagc gccgggaggt cgtcataggg caacgaacca ccctcgccga
     1501 tggcgcgtag cacgtcccgc ggctgcgcgg tgggacgccg tcgcccgccc atctcatcga
     1561 ggtcgcccgg cgctcccgcg tcgtcgaacc cgcccggatt tgtggagctg gagtcgtcct
     1621 ggttcggcat ggcctcagtg taacaagcga ctagggacgc gcccgcgaga gatcgatgtc
     1681 ctcgggcttt gggtcccgtt cgaggagcgc cgcgaccgcg gcgctcgggg cgacgccccc
     1741 gtacaggatc gagtgcaggc cctccgcgat cggca
//
LOCUS       DAORPG010000450         1772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8775_length_1772_cov_3.076878, whole genome shotgun sequence.
ACCESSION   DAORPG010000450 DAORPG010000000
VERSION     DAORPG010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8775_length_1772_cov_3.076878"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLX85_09050"
     CDS             <1..212
                     /locus_tag="PLX85_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63351.1"
                     /translation="VDWGPWGGGGMVSPELLEQYQRRGISVIDPEAGVRAFLDELRDG
                     AREDAQVILMSGAPEAVSVAAKSTR"
     gene            209..>1772
                     /locus_tag="PLX85_09055"
     CDS             209..>1772
                     /locus_tag="PLX85_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRC63352.1"
                     /translation="MSEIAIIGMACLFPGAPDLATYWDNICNGVDAISDAPPERLDSL
                     YFDEGSSATDRLYARRGGFVDQYATFDATTFGVMPVAAMGAEPDQLLTLQVAQRALED
                     AGYSSRDFPRERTSIILGRGNYFGAGMLRLNDHVRVAEQLVLSLRTVAPELDEQRLAE
                     IKADFQSRAGAFAPDTAIGLVPNLVASRVANRLNLRGGAYTIDAACASSLVAVDQACH
                     ELTSGRSEMAIAGGVHLCQDPNFWSVLCQLGAVSRSQQIRPFDRRADGLLVSEGIGLL
                     VLKERRRAERDGDRIYAVIRGSGVSSDGRSASIMAPAVEGQLLAISRAWGASGLDPAS
                     IGLIEAHGTATMAGDAAELETLERFFGSQAPTGERIVLGSVKSMIGHAMPAAGAAGLI
                     KAALAVYHGVLPPSIHCEEPRDELAAGHFEVLSQARTWETPVRRAGVNAFGFGGVNSH
                     VILEAATSRPMPARVGASEPAPDVLFLAAANQEELLALIDRGERRTSGGAWRLAVFDP
                     TPDRIAAARAAVE"
BASE COUNT          273 a          565 c          673 g          261 t
ORIGIN      
        1 ccgtcgactg gggcccgtgg ggcggcggtg ggatggtctc tccggagctg ctggagcagt
       61 accagcgacg gggcatcagc gtgatcgatc ccgaggccgg tgtacgcgcg ttcctcgacg
      121 agctgcgcga cggggcgcgg gaggacgcac aggtgatcct gatgagcggc gcgccagaag
      181 ccgtctccgt ggcagccaag agcacgcgtt gagcgagatc gccatcatcg gaatggcgtg
      241 cctgttcccg ggcgcgcccg acctcgcgac gtactgggac aacatctgca acggcgttga
      301 cgccatcagc gacgcgcccc cggagcgcct cgacagcctg tacttcgatg aagggtcgtc
      361 ggcaacggac cggctgtacg cgcggcgcgg cggtttcgtc gaccagtacg cgacgttcga
      421 cgcgacgacg ttcggtgtga tgccagtcgc cgcgatgggg gcagaaccgg accagctgct
      481 cacgctgcag gtggcacagc gcgcgctcga ggacgcggga tacagctccc gcgacttccc
      541 gcgcgaacgg acgagcatca tcctcggccg cgggaactac ttcggtgcgg ggatgctgcg
      601 gctgaacgac cacgtgcgcg tggcggagca gctggtgctg agcctgcgca cggtggctcc
      661 tgaactcgat gagcagcggc tggccgagat caaggcggac tttcagtcgc gcgccggggc
      721 attcgcgccg gacacggcga tcggcctcgt gccgaacctc gtggcgtcgc gggtggccaa
      781 ccggctcaac ctgcggggcg gcgcctacac gatcgacgcc gcgtgcgcga gttcgctcgt
      841 ggcggtggac caggcctgtc acgagctgac ctcggggcga tcggagatgg cgatcgccgg
      901 cggggtgcat ctgtgccagg acccaaactt ctggagcgtg ctctgccagc tgggcgccgt
      961 ttcgcgcagt cagcagatcc ggccgttcga ccgccgggcg gacggtctcc tcgtgagcga
     1021 gggaatcggg ctgctggtgc tcaaggagcg ccgtcgggcg gagcgggacg gtgaccggat
     1081 ttacgcggtc atccgaggca gcggcgtttc gagcgacggt cgcagcgcga gcatcatggc
     1141 tccagcggtc gagggtcagt tgctcgcgat ctcgagggca tggggagcga gcggcctcga
     1201 tccggccagc atcgggctga tcgaggcgca tggaacggcc acgatggccg gcgatgcggc
     1261 cgaactcgag acgctcgagc gtttctttgg gagtcaggcg ccgacgggcg agcgtatcgt
     1321 cctcggatca gtgaagtcga tgatcggcca tgcgatgccg gcggccggtg cggccggtct
     1381 gatcaaggcg gcgctggcgg tgtaccacgg cgtgctgccg ccgagcattc actgcgagga
     1441 gccacgggac gaactcgcgg ccgggcactt cgaggtgctg tcgcaggcac ggacctggga
     1501 gaccccggtc cgtcgcgcgg gcgtgaatgc gttcgggttc ggaggggtca acagccacgt
     1561 gatcctcgag gcggcgacct cgcggccgat gcccgcacgt gtaggggcga gcgaaccagc
     1621 gccggacgtc ctcttcctcg cggccgcgaa ccaggaggaa ctgctcgcgc tcatcgaccg
     1681 gggagaacgg cgcacaagcg gcggcgcgtg gcgcctcgcg gtgttcgacc cgacgcccga
     1741 ccggatcgcc gccgcgcgag ccgcggttga ac
//
LOCUS       DAORPG010000451         1769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8804_length_1769_cov_3.369312, whole genome shotgun sequence.
ACCESSION   DAORPG010000451 DAORPG010000000
VERSION     DAORPG010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8804_length_1769_cov_3.369312"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PLX85_09060"
     CDS             <1..367
                     /locus_tag="PLX85_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRC63353.1"
                     /translation="RDPKWDSRVTGSACIECAFVAAGIFSATAFWGLSIWDVAAGIVL
                     VRAAGLEAWTRNADGWARLERFEAPAKVREDRAPTLRDWRQPLLIGTEEATAPFRVGE
                     RGPRWWWRVRLAARILTGR"
     gene            complement(415..1176)
                     /locus_tag="PLX85_09065"
     CDS             complement(415..1176)
                     /locus_tag="PLX85_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HRC63354.1"
                     /translation="MTTSMADLRNAWGPDDPFVLEALRDSHVQQLQLIYDSSNYVFVA
                     TLEHPEFGSGLGIYKPRSGEQPLHDFPYGTLHEREIAAYEASEAFGWRLVPPTVERDG
                     PQGIGSMQLFIAHDPQQHYFELRKDVAYHEQLIRLAVFDLVMNNADRKGGHVLLDALG
                     HVWGIDNALCFHHVDKLRTVIWDFAGVEVSEAVRADLGRMRDCLEARDGSTKALRDWI
                     AEAEVDAIVRRCDALLGHPVLPEMYPWRCVPWPLL"
     gene            complement(1214..>1769)
                     /locus_tag="PLX85_09070"
     CDS             complement(1214..>1769)
                     /locus_tag="PLX85_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63355.1"
                     /translation="VEALLDVFQWVQPQELLICAALAEAWENPRVGGQGRPDPRVTSE
                     RELAHLNTRVELPPPSIGVLPEVAEALQIEAAPELYRAMARWPRYLEPVWDELQHMAA
                     YPDFRRRGRALYFYARSSSRFLARPLEASRETLAAQGLSDEELGHVSATLDASLPALA
                     TMMMHCCAMRVGLGITAREVVTTS"
BASE COUNT          253 a          664 c          585 g          267 t
ORIGIN      
        1 ccgtgaccca aagtgggact cacgcgtcac cggctcggcc tgcatcgagt gtgctttcgt
       61 cgctgccggc atcttttcgg ccaccgcctt ctggggcctc tcgatctggg acgttgcggc
      121 cggcatcgtc ctcgtccgcg ccgccggcct cgaggcgtgg acccgcaacg ccgacggctg
      181 ggcgcggctc gaacgcttcg aggcccccgc gaaggtccgc gaggaccgcg ctccgacgct
      241 ccgcgactgg cgccagccct tgctgattgg cacggaggag gcgaccgccc ccttccgcgt
      301 cggcgagcgt ggcccgcgct ggtggtggcg cgtccgcctc gccgctcgga tcctcaccgg
      361 ccgctgacga gggcccttcg accctccgag gtcagaccgc ggttcgaggc gacctcagag
      421 cagcggccat ggcacgcagc gccacgggta catctccggc agcaccggat gcccgagcag
      481 ggcgtcgcag cgccgcacga tcgcatccac ttcggcctcg gcgatccagt cgcgcagcgc
      541 cttcgtcgag ccgtcgcgcg cctcgaggca gtcacgcatc cgcccgaggt cggcgcgcac
      601 tgcctcggac acctcgacgc cggcgaagtc ccagatcacc gtgcgcagct tgtccacgtg
      661 gtggaagcac agcgcgttgt cgatccccca cacgtggccc agcgcgtcga ggagcacgtg
      721 cccgcccttg cggtccgcgt tgttcatcac gaggtcgaac accgcgaggc ggatcagctg
      781 ctcgtggtag gccacgtcct tgcgcagctc gaagtagtgc tgttgcgggt cgtgcgcgat
      841 gaacagctgc atcgacccga taccctgcgg tccgtcccgc tccaccgtcg ggggcacaag
      901 ccgccacccg aacgcctcgg acgcttcata tgcggcaatc tcgcgctcgt gcagcgtgcc
      961 gtacgggaag tcgtgcagcg gctgctcgcc cgaccgcggc ttgtagatgc cgagcccgct
     1021 gccgaactcc gggtgttcga gcgtggccac gaagacatag tttgaggagt cgtagatcag
     1081 ctgcagctgc tggacgtgcg agtcgcgcag tgcctcgagg acgaacgggt cgtccggccc
     1141 ccacgcattc cgcaggtcgg ccatgctggt cgtcacgccc tcgattgtac cgagggctga
     1201 gccggggccc gcatcaggag gtcgtcacca cctcgcgtgc tgtgatcccg agcccgacgc
     1261 gcatcgcgca gcagtgcatc atcatcgtcg ccagcgccgg caacgaggcg tccagcgtcg
     1321 cgctcacgtg tcccagctcc tcgtccgaca acccctgtgc cgcgagcgtc tcgcgactcg
     1381 cttcgagcgg ccgcgccagg aaccgcgacg aggatcgcgc gtagaaatac agcgcccgcc
     1441 cacgccgccg gaagtcgggg tacgccgcca tgtgctgcag ctcgtcccag accggctcga
     1501 ggtaccgcgg ccagcgcgcc atcgcgcggt acagctccgg cgccgcctcg atctgcagcg
     1561 cctcggccac ctcgggcagc acgccgatgg acggcggcgg cagctcgacc cgcgtgttga
     1621 ggtgcgcgag ctcgcgttcc gaggtcaccc gagggtctgg ccggccctgc ccgccgacgc
     1681 gcggattctc ccaggcctcg gccagcgccg cgcagatcag cagctcctgt ggctgcaccc
     1741 actggaacac gtcgagcagc gcctcgacc
//
LOCUS       DAORPG010000452         1769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8806_length_1769_cov_3.027421, whole genome shotgun sequence.
ACCESSION   DAORPG010000452 DAORPG010000000
VERSION     DAORPG010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8806_length_1769_cov_3.027421"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLX85_09075"
     CDS             <1..294
                     /locus_tag="PLX85_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63356.1"
                     /translation="GRERHLLDLGLVPGQPGEREWLSRRAAAVHFQSDDAASGFAELM
                     VGLRGCSGPGLDDLATDLVGTRGSVLIVGSLYTVAEAREHLLGITGDRAFGLR"
     gene            324..1583
                     /gene="fabF"
                     /locus_tag="PLX85_09080"
     CDS             324..1583
                     /gene="fabF"
                     /locus_tag="PLX85_09080"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRC63357.1"
                     /translation="MSGEYGRRRVVITGMGVLSPLGNSVDEMWRRVVAGESGIQRLTL
                     VDPTEYPCQVGGEVRDFDPTEYMDRKDARRMARFSQLAVAASRQAVAHAALDLEGMDR
                     QRVGIVCGNGGGGLPETDATMRTILARGGSKVDPLYVAKFLPNMAAGNVSIQLGLHGY
                     TNTVVTACAAGTQALGDATEVIRRGHADVMIAGGAEAGISELGLAAFCAMRALTTSYN
                     DRPEASSRPFDRDRDGFAPAEGAGMFVLEELEHARARGAEPLAEIVGYGVSADAQFLV
                     APPDDGSGAARAMRAALADADMGLDEVDYISAHATATDLGDLAETRAIRSVFGERAYQ
                     IPVSALKSQIGHLLGAAGGVETAAIVQTIRTSTIAPTINLEHPGEDCDLDYVPNVARA
                     ADVRVAMKNSFGFGGQNAVLILRRFEG"
BASE COUNT          248 a          591 c          678 g          252 t
ORIGIN      
        1 ggtcgtgagc gccacctgct cgatctcggg ctggtgcccg gacagcccgg cgagcgggag
       61 tggctgtcac gtcgcgccgc cgcggtgcac ttccagtccg acgacgccgc ctccggtttc
      121 gccgagttga tggtcggtct gcgcggttgc tcggggcccg gtctcgacga cctcgcgacc
      181 gacctcgtgg ggacgcgggg cagcgtcctg atcgtgggca gtctctacac ggtggcggag
      241 gcgcgggagc acctgctcgg catcaccggc gaccgcgcct tcgggctacg ctagactcgt
      301 ccgagacaga gggatggtgg cggatgagcg gtgagtacgg tcgccgaaga gtcgtcatta
      361 ccgggatggg ggtgctgtcg cccctcggca acagcgtgga cgagatgtgg cggcgcgtgg
      421 tggccgggga gtccggcatc cagcgactga cgctcgtgga ccccacggag tacccgtgcc
      481 aggtcggcgg cgaggtccgc gatttcgacc cgacggagta catggaccgc aaggacgcgc
      541 gccgcatggc gcgcttctcg cagctcgcgg tcgccgcctc gaggcaggcc gtcgcccatg
      601 ccgcgctcga cctcgagggc atggaccggc agcgcgtggg gatcgtgtgc gggaacggtg
      661 gcggcgggct gccggagacg gacgcgacga tgcgcaccat cctcgcgcgg ggcggctcga
      721 aggtggaccc cctgtacgtg gcgaagttcc tgccgaacat ggcggccggc aacgtctcca
      781 tccagctggg gctgcacggc tacacaaaca ccgttgtcac cgcctgtgcg gccggcacgc
      841 aggccctcgg cgacgcgacc gaggtgatcc gccgcggcca tgcggacgtg atgatcgcgg
      901 gcggcgcgga ggcgggcatc agcgagctgg ggctcgccgc gttctgcgcg atgcgcgcgc
      961 tgacgacctc gtacaacgac cgccccgagg cgtcctcgcg tccgttcgac cgcgaccgcg
     1021 acggcttcgc gccggccgag ggggcgggca tgttcgtgct ggaggagctg gagcatgcgc
     1081 gggcgcgcgg ggcggagccg ctggcggaga tcgtgggcta cggcgtcagc gcggacgcgc
     1141 agttcctcgt cgcgccgcca gacgatggca gcggcgcggc gcgggcgatg cgcgcggcgc
     1201 tggcggacgc ggacatgggc ctcgatgagg tcgattacat ctcggcgcac gcgacggcga
     1261 cggacctcgg cgacctcgcg gagacgcggg cgatccgcag cgtgttcggg gagcgggcgt
     1321 atcagatccc ggtgtcggcg ctgaagtcgc agatcgggca cctgctgggc gccgcgggcg
     1381 gcgtcgagac ggcggcgatc gtgcagacga tccggacgag cacgatcgcg cccacgatca
     1441 acctcgaaca cccgggcgag gactgcgacc tcgactacgt gcccaacgtg gcgcgcgccg
     1501 cggacgtgcg cgtagcgatg aagaactcgt tcgggttcgg cgggcagaac gcggtgctga
     1561 tcctgcggcg attcgagggg tagcgggacc agctcgccga cggtggcttc acacaccgcg
     1621 tatcctccgc tcgatgaaac ttcccctcct cgcagtgctc gctctggctg ctgtcctcgc
     1681 cgccgggagc gcgtgcagca gcgggggcgc ctcgacgtcc actcccactg ttgaggtcac
     1741 cgcgaccatc gaggccgtcg caacgcctg
//
LOCUS       DAORPG010000453         1768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8809_length_1768_cov_3.507297, whole genome shotgun sequence.
ACCESSION   DAORPG010000453 DAORPG010000000
VERSION     DAORPG010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8809_length_1768_cov_3.507297"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLX85_09085"
     CDS             complement(<1..227)
                     /locus_tag="PLX85_09085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702877.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRC63358.1"
                     /translation="MATGVVRQVISTVVDVEFPGGDLPEIYNAVAIDMSGKTLMTEVQ
                     QHLGNNWVRCLALASTDGLQRGASATDTGAP"
     gene            complement(315..1199)
                     /gene="atpG"
                     /locus_tag="PLX85_09090"
     CDS             complement(315..1199)
                     /gene="atpG"
                     /locus_tag="PLX85_09090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRC63359.1"
                     /translation="MAGAGAVRAIRSRIRSFESAAKVTTAMQLVAASKMRRAQDRALA
                     SRPYSDRMRSVLAQLSGYAASADPSELPPLLQQRPVKHFAYVHITADRGLAGGLNANM
                     NRAGASLVLEHQPLVERVSIAAVGRKGRDFFRRASFPMLAEFTQLGDYPGMAQILPIA
                     RVIMDDYVNGEIDQVFIGYQRFVNTAVQRPTVRQILPVTAPEGDTGPSVDFLFEPSPQ
                     QLLETLLPRYVEMQIYEAVLDAAASEQSARMVAMRAATDAANEMVADLTLTYNKARQE
                     LITGELLDIVGGSAALEA"
     gene            complement(1243..>1768)
                     /locus_tag="PLX85_09095"
     CDS             complement(1243..>1768)
                     /locus_tag="PLX85_09095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641757.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRC63360.1"
                     /translation="IPTNVISITDGQIFLETDLFNAGQRPAINPGISVSRVGGSAQTR
                     AMRSVAGTLRLDLSQYRELQAFAQFGTEDLDAATRQQLLRGERLTRLLVQPQYQPLSL
                     AQQVTILYAGVRGFLDDVPVEKVADFERAFHEYMRANAAPVIDAITSTGLLEQATEEQ
                     LRTAITDFKASVAY"
BASE COUNT          251 a          516 c          707 g          294 t
ORIGIN      
        1 atcggggcgc cggtgtcagt ggcggaggca ccgcgctgga ggccgtcggt ggaggcgagg
       61 gcgaggcagc ggacccagtt gttgccgagg tgctgctgga cctcggtcat gagggtcttg
      121 ccgctcatgt cgatggcgac ggcgttgtag atctcgggga ggtcgccgcc ggggaactcg
      181 acgtccacga cggtgctgat gacttgcctg acgactccgg ttgccatcgt tgcggtcctt
      241 tcgcggccct ccgggccggg ggtcctttcg cggcccgcgg gcggttcaac gcccggaggc
      301 acgactctcg ttcgttacgc ctcgagggcg gcggaaccgc ccacgatgtc gagcagctcg
      361 ccagtgatga gttcctggcg ggccttgttg taggtgagtg tgaggtcggc caccatctcg
      421 ttcgcggcgt ccgtggcggc gcgcatggcg accatgcgcg cggactgttc ggacgcggca
      481 gcgtcgagga cggcctcgta gatctgcatt tcgacgtagc ggggcagcag cgtctcgagg
      541 agctgctgcg gcgacggctc gaagaggaag tcgacgctcg gacccgtgtc gccctcgggg
      601 gcggtgacgg gcaggatctg gcgcacggtg ggccgctgga cggcggtgtt cacgaaacgc
      661 tggtagccga tgaagacctg gtcgatctcg ccgttcacgt agtcgtccat gatgacgcgg
      721 gcgatcggca ggatctgagc catacccggg tagtcgccga gctgcgtgaa ctcggcgagc
      781 atcgggaagc tggcgcggcg gaagaagtcg cggcccttgc ggccgacggc tgcgatggag
      841 acgcgctcga cgagcggctg gtgctcgagg acgagcgagg cgcccgcgcg gttcatgttg
      901 gcgttgaggc cgccggcgag ccctcggtcg gcggtgatgt gcacgtaggc gaagtgcttc
      961 accggacgct gctggagcag cgggggcagc tcggaggggt cggcgctggc ggcgtagccg
     1021 gagagctgag cgaggacgct gcgcatgcgg tcggagtacg gacgcgaggc gagcgcgcgg
     1081 tcctgcgcgc ggcgcatctt cgatgcagcg acgagctgca tcgcggtggt gaccttcgcc
     1141 gcgctctcga aagagcggat gcggctacgg atcgcacgga ctgcaccggc acccgccatg
     1201 ttgtcctcgc tctacgcttt ctgcgtcctc gtccctcggc ggctagtagg cgaccgaggc
     1261 cttgaagtcg gtgatggcgg tgcgcagctg ctcctcggtg gcctgctcga ggaggccggt
     1321 cgacgtgatg gcgtcgatga cgggagcggc gttggcgcgc atgtactcgt ggaaggcgcg
     1381 ctcgaagtcg gcgaccttct cgacggggac gtcgtccagg aagccgcgga cgccggcgta
     1441 gaggatggtg acctgctgcg ccaggctgag cggctggtac tgcggctgca cgaggaggcg
     1501 ggtcaggcgc tcacctcgga ggagctgctg gcgggtggcc gcgtcgaggt cttccgtacc
     1561 gaactgggcg aaggcctgca gctcgcggta ctgggagagg tcgaggcgga gggtaccggc
     1621 gaccgaccgc atggcgcggg tctgggcgga accgcccacg cgcgagaccg agatgccggg
     1681 gttgatggcg ggccgctgac cggcgttgaa gaggtcggtc tccaggaaga tctgaccgtc
     1741 cgtgatcgag atgacgttcg tcgggatg
//
LOCUS       DAORPG010000454         1766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8829_length_1766_cov_3.034483, whole genome shotgun sequence.
ACCESSION   DAORPG010000454 DAORPG010000000
VERSION     DAORPG010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8829_length_1766_cov_3.034483"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="PLX85_09100"
     CDS             complement(<1..768)
                     /locus_tag="PLX85_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC63361.1"
                     /translation="METSESRVLVECGLNQEREFAERNWDPFPVDPRSIDAVVLTHAH
                     LDHSGYLPRLARAGFSGPVWCTPATADLASIVLADAARIAVEDADYKRRRHEREGRRG
                     PHPETPLYTPVDVRRAVALLRTIGYHTPWTVAPGIRAEFRDAGHILGSAILTLRVASE
                     AGERVITFSGDLGRGANPMLRDPEPPPRSDYLVVESTYGDRDHPDEDVARELERVINE
                     TVEAGGNVVIPAFAIGRTQDMLYHLRRLLLERRIPRLL"
     gene            complement(872..1402)
                     /locus_tag="PLX85_09105"
     CDS             complement(872..1402)
                     /locus_tag="PLX85_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRC63362.1"
                     /translation="MRILVAVDASEQAERVVAAAAGLLRNVKDPEIRLLTVVDDADIH
                     ESTATTLPQGTPTPLGSWSGSSYNIPTPSSPHLMEDRSQALTRIHGELQSRDATLAAH
                     HLPGQTIESRVVSGSKPAPAILEAAKEFDAELIVMGTRGRRGISRILMGSTAEAIVRE
                     SPIPVVVIGDAVKAAS"
     gene            1545..>1766
                     /locus_tag="PLX85_09110"
     CDS             1545..>1766
                     /locus_tag="PLX85_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC63363.1"
                     /translation="MVHFALRTLLYDRVRFLIAVGGVAFAILLMLVLRGIMDGTVAKS
                     TAYVDHVGADVFVAQQGVQHFALTSSVVPV"
BASE COUNT          271 a          560 c          649 g          286 t
ORIGIN      
        1 cagcagccgc gggatgcgcc gctcgaggag caggcggcgc aggtggtaca gcatgtcctg
       61 cgtgcgaccg atggcgaatg ccgggatgac gacgttgccg cccgcctcga cggtctcatt
      121 gatcacccgc tcgagttcgc gcgcgacgtc ctcgtccggg tgatcgcggt cgccataggt
      181 cgactcgacg acgaggtagt cggatcgcgg cggtggctcc gggtcgcgga gcatcgggtt
      241 cgcgccgcgt ccgaggtcgc cagagaaggt gatcacccgc tcgcccgcct cggacgccac
      301 gcgcaaggtg aggatggccg agccgaggat gtgaccggcg tcgcggaact ccgcacggat
      361 gccgggggcg accgtccacg gggtgtggta gccgattgtg cgcaggagcg cgaccgcgcg
      421 gcggacgtcg actggcgtgt agagcggcgt ttcggggtga gggccgcgcc ggccctcgcg
      481 ttcatggcgg cgacgcttgt agtcagcgtc ctcgacggcg atgcgggcgg catcggcgag
      541 cacaatgctt gcgaggtcag cggtcgcggg agtgcaccac acggggccgg aaaatccggc
      601 gcgagcgagc ctcgggagat agccggagtg atcgaggtgg gcgtgggtga ggacgacggc
      661 gtcgatggaa cgcgggtcga ccgggaacgg atcccagttg cgctcggcga actcgcgctc
      721 ctgattcagg ccgcactcga cgaggacgcg gctctccgag gtctccaaca ggaagcgcga
      781 cccggtgaca tttccggccg cgccgagaaa gcggagggcg ggacggtcat tccgacgtga
      841 aatgagaccc tcccgtcctc gatggacgct gctagctcgc tgccttcacc gcgtcgccga
      901 tgactacgac ggggatcggc gactcacgca caatcgcctc cgcggtgctg cccatgagaa
      961 tacgcgagat tccacgccgt ccgcgcgtgc ccatcacgat caactcggcg tcgaactcct
     1021 tcgcggcctc gaggatcgcc ggagccggct tcgagcccga caccacccgc gactcgatgg
     1081 tctggccggg gaggtggtgc gcggccagcg tggcgtcacg cgactgcagc tcaccgtgga
     1141 tacgggtgag ggcctggctg cgatcctcca tcaggtgggg cgacgatggt gtcgggatgt
     1201 tgtagctact gccgctccac gaaccgagcg gggttggggt gccctggggg agcgtcgtcg
     1261 cggtcgactc gtggatgtcg gcgtcatcga cgactgtcag caggcggatc tccggatcct
     1321 tcacgttgcg cagcaacccg gcagccgcgg cgacgactcg ttcggcctgc tcgctagcat
     1381 ctacagcaac aagaatgcgc atgatttcct ccgatcgaac ccagcgtctc gccgggggtt
     1441 tagggctgcg agggccaaac gtcccctcgg acgagggaac gtcggtcctc gcggcgggcc
     1501 cccagccacc ggctcgaggg ggccccatcg ggtactctcg gcggatggtt cactttgccc
     1561 tgcggacact gctgtacgac cgagtgcggt tcctgatcgc ggtgggcggg gtggcattcg
     1621 cgatcctgct gatgctcgtg ctacggggga tcatggatgg caccgtggcg aagtcgacgg
     1681 cgtacgtgga ccacgtcggg gctgacgtct tcgtcgcgca gcagggcgtg cagcacttcg
     1741 cgctcacctc gtcggtcgtc ccggtg
//
LOCUS       DAORPG010000455         1762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8865_length_1762_cov_3.838313, whole genome shotgun sequence.
ACCESSION   DAORPG010000455 DAORPG010000000
VERSION     DAORPG010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8865_length_1762_cov_3.838313"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1192)
                     /locus_tag="PLX85_09115"
     CDS             complement(107..1192)
                     /locus_tag="PLX85_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HRC63364.1"
                     /translation="MADHVYLLVGTHKGGFILEGNPERNDWKIQGTCFEGTDVQHLTM
                     DTREGRNRLYAAVNNPWWGPGVRMSDDMGQTWSEPSAPPKFEESLGLTLERVWQITPS
                     RDHSPGTIFVGVDPAALFRSDDHGETWSDVRGLTTHPTRGQWAPGAGGMMVHGILVHP
                     YDARRMQVAISAAGCFETIDAGENWMPRNKGVLADFLPEQYPEVGQCVHHLQNHPSRP
                     DLLYQSNHCGVYRSNVGGEQWTDLSEGLPSRFGFACVVHPLENDTVYVVPMVGPELRA
                     PVDAQLAVYRNREREGWERLTTGLPQDHAYPTVYRQAMVADQLDPTGLYLGTSNGQIY
                     SSVDAGDSWQSVMTDLPSVYSIEAVAL"
     gene            complement(1233..1490)
                     /locus_tag="PLX85_09120"
     CDS             complement(1233..1490)
                     /locus_tag="PLX85_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HRC63365.1"
                     /translation="MRERTPDERPDSWPILRSFKLWAILTSVLLIIIFTLQNTDSVEM
                     RILIWSFTASGAFLVLVAVALGFAAGWVFRSARGHGFDLFG"
     gene            complement(1575..>1762)
                     /locus_tag="PLX85_09125"
     CDS             complement(1575..>1762)
                     /locus_tag="PLX85_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63366.1"
                     /translation="PLFALPIQGGRIMAAYATHLGAEAKEAFRDRYLDLVRRANGADL
                     LHEDYMARADLIPPPEA"
BASE COUNT          269 a          550 c          655 g          288 t
ORIGIN      
        1 gcgcagccat ggtcgtggtt caaggacaca ttcctgtagg ggggcgcaat cgatacgccc
       61 cggacgaaca tgctcgaccg gggcgcatcg gtgagtgcga gcgttcctac agagcgaccg
      121 cctcgatgga gtagacgctc gggaggtcgg tcatcaccga ctgccacgag tccccggcgt
      181 cgacgctgga gtagatctgg ccgttgctcg tgccgaggta gaggcccgtg gggtcgagct
      241 ggtcggcgac catagcctgg cggtagacgg tcgggtacgc gtgatcctgc ggcagaccgg
      301 tggtgagccg ctcccagccc tcgcgctccc ggttacggta gacggcgagc tgcgcgtcga
      361 cgggggcacg cagctccggg cccaccatgg ggacgacgta gaccgtgtcg ttctcgaggg
      421 gatgcacgac gcaggcgaag ccgaagcgag acggcaggcc ctcgctgagg tcggtccact
      481 gctcgccgcc gacgttcgag cggtacacgc cgcagtggtt cgactggtag agcaggtcgg
      541 ggcggctcgg gtggttctgc aggtggtgga cgcactgccc cacctcgggg tactgctccg
      601 ggaggaagtc ggcgagcacg cccttgttgc gcggcatcca gttctcgccg gcgtcgatgg
      661 tctcgaagca gccggccgcc gagatggcga cctgcatgcg ccgggcgtcg taggggtgca
      721 cgaggatgcc gtgcaccatc atgccgcccg cgccgggcgc ccactggccg cgggtgggat
      781 gagtcgtgag gcctcggacg tcggaccacg tctcgccatg gtcgtcgctg cggaagagcg
      841 cggcggggtc gacgccgacg aagatggtgc cgggggagtg gtcacgcgag ggggtgatct
      901 gccacacccg ctcgagcgtc aggccgagtg attcctcgaa cttcgggggc gccgagggct
      961 cggaccaggt ctggcccatg tcgtcgctca tgcggacgcc gggtccccac cacgggttgt
     1021 tcaccgcggc gtagaggcgg ttgcggccct cgcgggtgtc catcgtgagg tgctggacgt
     1081 cggtgccttc gaagcacgtg ccctggatct tccagtcgtt gcgctccggg ttgccttcga
     1141 ggatgaagcc gcccttgtgg gtgccgacga gcaagtagac gtggtctgcc atcgatgtcc
     1201 cctctgtccc gattccgatc taccggcgca ctttacccga agaggtcgaa cccgtgtccg
     1261 cgggcgctgc ggaacaccca gccggcggca aagccgaggg ccaccgccac gagcacgaga
     1321 aaggccccgg aggccgtgaa cgaccagatc aggatgcgca tctcgacgga gtcggtgttc
     1381 tgcagcgtga agatgatgat gagcagtacc gaggtcagga tcgcccacag cttgaaggac
     1441 cgcaggatcg gccaggagtc tggccgctcg tccggggttc gctctcgcat cggctgcacc
     1501 tttctcgttc gggccacgtt cctgcctcag cgagaagata gcgcagcgcg tggcgggagg
     1561 tgcgagcggc gtcgttacgc ctcgggcggc gggatcaggt cggctcgcgc catgtagtcc
     1621 tcgtgcagca ggtcggcgcc attggcgcgg cggaccaggt cgaggtagcg gtcgcggaac
     1681 gcctctttcg cctcggcgcc gaggtgggtg gcgtaggccg ccatgatgcg gccgccctgg
     1741 atcggcaacg cgaacagcgg ct
//
LOCUS       DAORPG010000456         1749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8989_length_1749_cov_2.880756, whole genome shotgun sequence.
ACCESSION   DAORPG010000456 DAORPG010000000
VERSION     DAORPG010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8989_length_1749_cov_2.880756"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PLX85_09130"
     CDS             complement(<1..457)
                     /locus_tag="PLX85_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC63367.1"
                     /translation="MPDQLLPGLWWLHGTRGCNVYLQRTLDGGAVIVDTGFGSNARIV
                     HEQVRDLGFEGRVRAILLTHRHVDHAGGAAALSELLAAPVVAGRGDCHTHEGATTLAR
                     DWHPPALIRRPVGALLGIGGSHRPVEVARPVDIECEVAPGILAIPAPGHT"
     gene            652..1692
                     /locus_tag="PLX85_09135"
     CDS             652..1692
                     /locus_tag="PLX85_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRC63368.1"
                     /translation="MKIGVHLSWDAPDDWASAARFAVEAERLGVDSLWTGEAWGWDGA
                     TPLAYVAGQTSRVRLGTSILKIGARTPALTAMTALSLASMSGDRFVLGLGTSTPQIME
                     GWDGVPFEAPLRRTRELVELVRLMTSGQRVTYAGQVYQAPLPGEARPMRSSAPPRRVP
                     IYIAAVGPQNLRLTGELADGWIGNCFVPETGAAFLDPLRAGAERAGRSLSDLELQAPV
                     SLEFDDDLDAVTKRHARGYAFTFGAMGAPERNFYVAAFARQGFEVVREVHRLWLEGHR
                     EEAQELVPPELAWRTNLLGTDATVKERLRAYRSAGIDSLRVALRGANNRERLGDLERL
                     LGLVREVEREAP"
BASE COUNT          235 a          484 c          780 g          250 t
ORIGIN      
        1 gggtgtggcc gggcgcgggg atggcgagga tgccgggcgc gacctcgcat tcgatgtcga
       61 ccgggcgtgc gacctcgacg gggcggtggg agccgccgat accgaggagc gcgccgacgg
      121 ggcgccggat gagcgcgggt ggatgccagt cgcgggcgag ggtggtggcg ccctcgtggg
      181 tgtggcagtc gccacggcca gcgacgacgg gcgcggcgag cagctcgctg agcgcggcgg
      241 ccccgccagc gtgatcgacg tggcggtggg tgaggaggat ggcgcggaca cggccctcga
      301 agccgaggtc gcgcacctgc tcgtggacga tgcgggcgtt ggagccgaag ccagtgtcaa
      361 cgatgactgc tccgccatcg agggtgcgct gcaggtagac gttgcagcct cgggtgccgt
      421 gaagccacca gaggccgggg aggagctggt cgggcaacgt gttgtcgatc ctcggtgggt
      481 tgttcgcggt ggggccggta ggcggccctt gcccctcaag ggagaggacc cagtgggcgg
      541 ggcgattgtg gatggctcag ttcggcaggc gcgggaggta cggtcaagcc gagtgcagtc
      601 cgcggcggcg gctatgatgc ctcgacggcc ggaccggtgg aggggcagag aatgaagatc
      661 ggggtgcacc tgagctggga cgcgccggat gactgggcga gcgcggcgcg gtttgcggtg
      721 gaggccgaac ggctaggggt ggactcgctg tggaccggcg aggcgtgggg gtgggacggg
      781 gcgacgccgc tggcgtatgt ggccgggcag acctcgaggg tccggctggg gacgagcatc
      841 ctgaagatcg gggcgcggac gccggcgctg acggcaatga cggcgctgtc gctggcctcg
      901 atgtcggggg accggttcgt gctcgggctg gggacgagca cgccgcagat catggagggc
      961 tgggacgggg tgccgttcga ggcgccactg cgccggacgc gcgagctggt ggagctggtg
     1021 cgtctgatga cgagcgggca gcgcgtgacg tacgcgggcc aggtgtacca ggcgcccctg
     1081 cccggcgagg cgcggccgat gcggtcgagc gcgccgcctc gccgggtgcc gatctatatc
     1141 gcggcggtgg ggccgcagaa tctgcggctg acgggcgagc tggcggacgg gtggatcggg
     1201 aactgcttcg tgccagagac gggcgccgcg ttcctcgacc cgctgcgcgc gggggcggag
     1261 cgggcggggc ggtcgttgtc ggatctggag ctgcaggcgc cggtgtcgct cgagttcgac
     1321 gatgacctcg atgcggtgac gaagcggcac gcacgcgggt acgcgttcac gttcggggcg
     1381 atgggcgcgc cggagcggaa cttctatgtc gcggcgttcg cgcggcaggg gttcgaggtg
     1441 gtgcgggagg tgcaccggct gtggctggag gggcaccgcg aggaggcgca ggagctggtg
     1501 ccgccggagc tggcgtggcg gacgaatctg ctcgggacag acgcgacggt gaaggagcgg
     1561 ctgcgggcgt accgctccgc cggaatcgat tcgttgcggg tggcactgcg cggcgcgaat
     1621 aaccgggaac ggctggggga cctcgagcgg ttgctggggt tggtgcggga ggtggagagg
     1681 gaggcgccgt aggcttgcgg gcggtccgac tgtctttttc ggaaacccac cctcccgccc
     1741 tggaacggg
//
LOCUS       DAORPG010000457         1749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_8990_length_1749_cov_2.851240, whole genome shotgun sequence.
ACCESSION   DAORPG010000457 DAORPG010000000
VERSION     DAORPG010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8990_length_1749_cov_2.851240"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..387
                     /locus_tag="PLX85_09140"
     CDS             148..387
                     /locus_tag="PLX85_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRC63369.1"
                     /translation="MRITAKGQVTIPQEIRERAGLLPNTEVEFLLDSEGVRIVKATTA
                     RRPTRGEKVIETLRRGRGHITMTTDELMALTRGEE"
     gene            384..788
                     /locus_tag="PLX85_09145"
     CDS             384..788
                     /locus_tag="PLX85_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012308480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRC63370.1"
                     /translation="MSPVLVDTNVLIDIFSDDPVWAGPSTDALRRLADSEILVVNPII
                     YAELSAAFDTIEALDEVLPDEMFRREHLPYEAAFLAGKVFLQYRRAGGSRRSPLPDCY
                     IGAHAAVAGYWLLTRDSARYGHYFPLLRLMSP"
     gene            888..1220
                     /locus_tag="PLX85_09150"
     CDS             888..1220
                     /locus_tag="PLX85_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HRC63371.1"
                     /translation="MAKFLVKAKYSAPDGVRGLLEDGGTERVTAVRALIESVGGTMEA
                     FYFALGEYDAYVIADVPSIEAGTALSLTVGASGLASTDTVTLITPEQVDAAVKLSPVY
                     DAPGKFDN"
     gene            1456..>1749
                     /locus_tag="PLX85_09155"
     CDS             1456..>1749
                     /locus_tag="PLX85_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63372.1"
                     /translation="MAVHSDAWERLRSLGHLAAVSSSEERPGRSPDPTAVDRRPPAAQ
                     PVAIVRYHLVTLGCAKNTVDSMRMESALRHARHTAVATPEDADVLFVNTCGFID"
BASE COUNT          330 a          599 c          538 g          282 t
ORIGIN      
        1 acaccttccg ccgcgccgaa tcgagggagc cagtcgccgt ctagcctcaa cgggcaacga
       61 acagcacgaa gcccgggtta cgcccgggct tcgtcgtggt gagggcgggc ctgcagtatt
      121 accatccaag gtaagaaggg cacacttatg agaatcacgg cgaaaggcca ggtgacgatc
      181 ccacaggaga tccgcgaacg tgcgggactg cttcccaata cggaagtcga gttcctgctc
      241 gacagcgagg gggtccgcat cgtcaaggca acaacggctc gacgtcccac ccgaggcgag
      301 aaggtgatcg agacgctgcg acgggggcgc ggccacatca cgatgacgac ggacgaacta
      361 atggctctga cgcgcggaga agaatgagcc cggtcctcgt cgacaccaac gtgctgatcg
      421 acatcttcag cgacgacccg gtgtgggcag ggccgtcgac cgacgcacta cggcggctcg
      481 ccgactccga aatcctggtg gtcaacccca tcatctacgc cgagctgtct gcggccttcg
      541 acaccatcga ggcactcgac gaagtgctgc cagacgagat gttcaggcgc gagcacctac
      601 cgtatgaagc cgcattcctt gccgggaagg tgttcctaca gtaccggcga gcgggcggat
      661 cacgacgttc gccgctgccc gactgctata tcggcgcgca tgccgcggtc gctgggtact
      721 ggctcctcac ccgagactcc gcgaggtacg gccactactt cccgttgctg cgcctcatga
      781 gtccctgacc gcagtcgcag tttgacgccc ttcgcccccg gccatacgtt gcgcagcgac
      841 acgcgggaag ccgcgaacga gtggggcaac gagaggggtc gagtgagatg gcgaaattcc
      901 tggtcaaggc aaagtacagc gcaccggacg gcgtgcgagg actcctcgag gacggcggca
      961 ccgaacgcgt gacggccgtg cgggcgctca tcgaatcggt cggcggcacg atggaggcgt
     1021 tctacttcgc cctcggcgag tacgacgcct acgtcatcgc cgacgtcccc agcatcgagg
     1081 cgggcaccgc cctctcgctc acggtcggcg ccagcggcct cgcctccacc gacaccgtca
     1141 ccctgatcac cccggagcag gtcgacgccg ccgtcaagct gtccccggtc tatgacgcgc
     1201 ccggcaagtt cgacaactga cgcgcacggt tccgaagacg aacggagggc gctcttcagc
     1261 gccctccgtt tagaaccgtc gtgcacgccg ccatttgaga actcggatgc acaccctccg
     1321 tcctggcggc ctgcgattgg tcgagggagg cgctcctcgg ccagctgctg aagtatgcgc
     1381 cggattaaca cgtccgtagc tatcgaaact cgctgatacg ttacagatcc atcacgaagg
     1441 ctcacctagc cgagcatggc cgtgcacagc gatgcttggg agcgcctgcg ttcgctcggt
     1501 cacctcgccg ccgtttcctc ctccgaggag agacctggac gatcgccgga tccgaccgcg
     1561 gttgaccgcc gcccgcccgc ggcccagcct gtagccatcg tgcgctatca cctcgtcacg
     1621 ctcggctgcg cgaagaacac cgtcgactcc atgcgcatgg agtccgccct ccgtcacgcg
     1681 cgtcacaccg ctgtcgcgac tcccgaggat gccgacgtcc tgttcgtgaa cacctgcggc
     1741 ttcattgat
//
LOCUS       DAORPG010000458         1747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9012_length_1747_cov_3.627069, whole genome shotgun sequence.
ACCESSION   DAORPG010000458 DAORPG010000000
VERSION     DAORPG010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9012_length_1747_cov_3.627069"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLX85_09160"
     CDS             <1..234
                     /locus_tag="PLX85_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC63373.1"
                     /translation="EGMSRAFVADLSPSGQRGRSFGWFHMATAVTALPASVIAGALWT
                     EVGPEAAFSFGAGCALLAFVLLMTVRAPRTALS"
     gene            complement(227..940)
                     /gene="radC"
                     /locus_tag="PLX85_09165"
     CDS             complement(227..940)
                     /gene="radC"
                     /locus_tag="PLX85_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRC63374.1"
                     /translation="MEEVGEPPRYQLRVAELPAEERPRERLLHLGPHALTTAELLAIL
                     LRTGSSREGVIEIASRLLRDHGGLRGLAAADLATLSGEHGMGDAKATTIAAAFELGRR
                     LALLGDEVRPTINGPGDIARLLQAEMELLQQEELRLLTLDTKHHVLASTMLYRGSVNA
                     AGGRVAEVFREAVRRNATAIAVAHNHPSGDPVPSRDDVAFTSALVRAGELIEVEVLDH
                     IVFGRGRYVSMRQQRLGFS"
     gene            complement(928..1533)
                     /locus_tag="PLX85_09170"
     CDS             complement(928..1533)
                     /locus_tag="PLX85_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63375.1"
                     /translation="MTTLAGELLEERRNHRRSVIRQAVVYTPCAVAVLVLLGISLWNV
                     AIGHYGALIAATVLALIAVAVVYQSLAALRDLGSQPVATTGMVRRAWTKGMFLGFIRS
                     HYILVQRAVFDISIVSYTQLNEGDEVEVRHWPHTKTVIEVRKLKVGTLGAARAEATRR
                     AFEPQPEPYVMPDATDMMMSSRYGGLWWFKLRDAFWRRWRK"
     gene            complement(1530..>1747)
                     /locus_tag="PLX85_09175"
     CDS             complement(1530..>1747)
                     /locus_tag="PLX85_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRC63376.1"
                     /translation="AATPTPPPATPAPTQAPAPRTYQVVSGDTLSIIANRFRGDLDQN
                     TYLQRLYQANNLGPTSVITVGQTINLP"
BASE COUNT          243 a          574 c          639 g          291 t
ORIGIN      
        1 gaggggatga gccgcgcctt cgtcgccgac ctctcgcctt ccgggcagcg aggtcgctcg
       61 ttcggctggt tccacatggc gaccgccgtc acggcgctcc ctgcaagcgt gatcgccggg
      121 gcgctctgga cggaggtcgg cccggaggcg gcgttcagct ttggcgcggg atgcgcgttg
      181 ttggccttcg tcctgctgat gacggtgcgc gcaccccgga ccgcactcag ctgaagccca
      241 gccgctgctg gcgcatcgag acatagcggc cgcgcccgaa cacgatgtgg tcgaggacct
      301 caacctcgat gagctcgccg gcgcgcacga gcgcgcttgt gaaggcgacg tcatctcggc
      361 tcggtacggg gtcgccgctg ggatggttgt gcgccaccgc gatcgccgtc gcgttgcggc
      421 ggacggcttc ccggaacacc tcggcgacgc ggccgccggc agcgttgacc gagccgcggt
      481 agagcatcgt gctggcgagc acatggtgct tcgtgtcgag ggtcaggagc cgcagctcct
      541 cctgctgcag caattccatc tccgcctgca gcaaccgagc gatgtcgccg ggcccgttga
      601 tggtggggcg cacttcgtcg ccgaggagtg cgagccgccg cccgagttcg aaggcggccg
      661 cgatcgtcgt tgccttggcg tcgcccatgc cgtgctcgcc gctgagcgtc gcgaggtcgg
      721 ccgccgccag gccgcgcaac ccgccgtggt cacgcagcag gcgtgaggcg atttcgatca
      781 cgccttcccg gctgctgccc gtgcggagca ggatcgcgag gagttcggcg gtcgtgaggg
      841 cgtgcgggcc gaggtgaagc agacgctcgc gcggccgttc ctcggccggg agttccgcta
      901 cgcggagctg gtagcgcggt ggttcgccta cttcctccat cgtcgccaga acgcatcccg
      961 gagcttgaac caccagaggc cgccgtagcg gctgctcatc atcatgtcgg tcgcgtctgg
     1021 catgacgtac ggctcgggct gcggctcgaa cgcgcggcgg gtggcttcgg cgcgcgcggc
     1081 gccgagcgtc ccgaccttga gcttgcgcac ctcaatgacg gtcttcgtgt gcggccagtg
     1141 acgcacttca acctcgtcgc cctcattgag ctgggtgtag gagacgatcg agatgtcgaa
     1201 gacggcccgc tgcaccagga tgtagtgcga gcggatgaag cccaggaaca tgcccttcgt
     1261 ccacgcccgc cggaccatcc ccgtggtcgc gaccggctgt gatccgaggt cgcgcagcgc
     1321 cgcgagggac tgatacacca ccgcgacggc gatcagcgcc aggacggtcg ccgcgatcag
     1381 cgcgccgtag tgaccgatgg cgacgttcca gagcgagatg ccgagcaaca cgaggacggc
     1441 caccgcgcac ggtgtgtaca cgaccgcctg gcggatgacg ctgcggcgat gattgcgccg
     1501 ctcctcgagc agctcacctg cgagcgtcgt catggcaggt tgatggtctg cccgactgtg
     1561 atgacgctcg tgggcccgag gttattggcc tggtagagcc gctgcaggta cgtgttctgg
     1621 tcgaggtcgc cgcggaaccg gttcgcgatg atagagagcg tgtccccgct gacgacctgg
     1681 tacgtacggg gcgcgggcgc ctgtgtcggg gcgggtgttg cggggggcgg cgtcggcgtg
     1741 gcggccg
//
LOCUS       DAORPG010000459         1746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9023_length_1746_cov_2.758723, whole genome shotgun sequence.
ACCESSION   DAORPG010000459 DAORPG010000000
VERSION     DAORPG010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9023_length_1746_cov_2.758723"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PLX85_09180"
     CDS             <1..472
                     /locus_tag="PLX85_09180"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRC63377.1"
                     /translation="TRVTERGARVGDRPGASMSGEEAVVAEFDAEGFAREWVAAWNAR
                     DVEAVVGHFTEDAQFFSPRAEEVTGSPVVSGRAALREYWQTATGHLDRLQFTLDHVVW
                     DGGERLAIVYNSEVGERRRRAVELLVIDAASGRARYGEAMYGAELRSTGMARPG"
     gene            complement(521..1165)
                     /locus_tag="PLX85_09185"
     CDS             complement(521..1165)
                     /locus_tag="PLX85_09185"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HRC63378.1"
                     /translation="MNETARHRGGLTLSRVDALTDGVFAIVATLLVLEIHVPELPEHA
                     TTSEVWHSLGDVAPSFVAFVFSFVTILVYWVNHESLSRVTTRYPYRLVWINLVLLLFI
                     SLIPFTTDFISEYPKEPVAVATYGLVMACSAATAVAGYWYVAFGANLMTPTVSIEVRR
                     RLLRRFGLGPVFYLAAALAAFLSVYVAIAVYIALPLAFFVPALQERVLEDLGEE"
     gene            complement(1249..>1746)
                     /locus_tag="PLX85_09190"
     CDS             complement(1249..>1746)
                     /locus_tag="PLX85_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63379.1"
                     /translation="FDIKHETLLYAAVLFAIGATVGVFARLLIQSEVGSDVEDYGLYM
                     ARVAQTVVASGVAALLGVVITVYGVAAINNSDVVPAPATSPAPAAAAPSTSTPAPTAL
                     ASSANNVRLESVFSFEEYPLAILLAAIFGLSPTLLLKRLEDATTRLKLDIKSIESSEG
                     ASPSS"
BASE COUNT          292 a          475 c          741 g          238 t
ORIGIN      
        1 gactcgggtg accgagcgag gcgctcgggt gggggaccgc ccgggcgcct cgatgtccgg
       61 ggaggaggca gtcgtggcgg aattcgacgc ggaggggttc gcgcgggagt gggtggcggc
      121 gtggaacgcg cgtgacgtcg aggcggtggt ggggcacttc acggaggatg cgcagttctt
      181 cagtcctcgg gcggaagagg tgacagggag ccccgtggtg tcgggccggg cggcgctgcg
      241 cgagtactgg cagacggcga cggggcacct cgaccggttg cagttcacgc tcgaccacgt
      301 ggtgtgggac ggcggcgagc ggctggcgat cgtctacaac agcgaggtgg gcgagcgacg
      361 gcggcgcgcg gtcgaactgc tcgtgatcga cgccgcctcg ggacgggcgc gttacggcga
      421 ggcgatgtac ggagctgagc tgcggtcgac ggggatggcg cggccgggat aggcagggcg
      481 cggtcggggg gccagtcccc gtgcgtttcc tcggacggcg ttactcctcg ccgaggtcct
      541 cgaggacgcg ctcctggagg gcggggacga agaaggccag cgggagcgcg atgtacacgg
      601 cgatagcgac gtacacggag aggaaggcgg cgagcgcggc cgcgaggtag aacacgggcc
      661 cgagaccgaa ccgacggagg aggcgccggc gtacctcgat gctgacggtc ggagtcatga
      721 ggttcgcgcc gaaggcgacg taccagtagc cggcgaccgc ggtggcggcg ctgcaggcca
      781 tgacgaggcc gtaggtggcg acggcgacgg gctccttcgg gtactcgctg atgaagtcgg
      841 tggtgaacgg gatcagcgag atgaagagca gcagcacgag gttgatccac acgagccggt
      901 aggggtagcg cgtcgtgacg cggctgaggc tttcatggtt cacccagtag acgaggatcg
      961 tgacgaagga gaagacgaag gcgacgaacg agggcgccac gtcgccgagg ctgtgccaga
     1021 cctcggaggt cgtggcgtgc tcggggagct cggggacgtg gatttcgagg acgagtagcg
     1081 tggcgacgat ggcgaagacg ccgtcggtga gggcgtcgac gcggctgagc gtcagcccgc
     1141 cgcgatgcct cgcggtctcg ttcatggtat gcgcccccga ttcacgctgc tggtgcgggc
     1201 gaatctacgt gggcggatgg cgggacgcaa cgcctcggac gccgcgtgtc agctcgaggg
     1261 ggaggcgccc tcgctcgact cgatgctctt gatgtcgagc ttaagccggg tggtggcgtc
     1321 ctcgaggcgc ttgaggagga gggtgggcga caggccgaag atcgccgcga gcaggatggc
     1381 gagggggtac tcctcgaagc tgaagaccga ctcgaggcgg acgttgttgg cggagctcgc
     1441 aagggcggtc ggggccggag tgctggtcga aggcgcggcg gcagcggggg ccggcgacgt
     1501 cgcgggggca gggacgacgt ccgagttatt gatggcggcg acgccgtaga cggtgatgac
     1561 cacgccgagg agcgcggcga cgccggaggc gacgacggtc tgcgcgacgc gcgccatata
     1621 gaggccgtag tcctcgacat ccgagccgac ctcggactgg atcagcaggc gggcgaagac
     1681 gccgacggtc gcgccgatgg cgaagagcac ggcggcgtaa agcagggtct cgtgcttgat
     1741 gtcgaa
//
LOCUS       DAORPG010000460         1737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9106_length_1737_cov_3.207491, whole genome shotgun sequence.
ACCESSION   DAORPG010000460 DAORPG010000000
VERSION     DAORPG010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9106_length_1737_cov_3.207491"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PLX85_09195"
     CDS             <1..772
                     /locus_tag="PLX85_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC63380.1"
                     /translation="HSIGATFHRIQFTPDLMPSDILGINYYSQRTGDFEFRPGPILAN
                     VILADEVNRATPRTQSALLEAMEERTVTVDGETRPLPDPFLVLATQNPIELEGTFPLP
                     EAQLDRFLLRLKVGYPTEAGEIEVLRRFEGASPLDALRPAVEAEELLRLRQVLPGIHV
                     EDAVAAYVVRLCRATRDHIAFDLGASPRASLALFRAARALAAMDGRDYVRPDDVKRLA
                     QPVLAHRLVLSSQTRLRGRSADDVLDEILHEVPVPVAD"
     gene            789..>1737
                     /locus_tag="PLX85_09200"
     CDS             789..>1737
                     /locus_tag="PLX85_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRC63381.1"
                     /translation="MRSVFRDSWVSVTIVLVLVGLAAQSVPLIGLGVLVFGTGGMARL
                     WARVSLEDVRYRRVLRERRVFMGETIDLRLRLANEKRLPVPWLEVREYLPEAMPAGGV
                     HVTPSGLPGTVLMQRSTSLGAHEQLEWPLTLDATERGFFRLGPTRLRSGDLFGIFERE
                     ETIRGGDSVVVYPRTYSLPELGIISARPFGERAGGRRIFEDPVRVVGVRDYFPGDPLK
                     RVDWNATARIGRLQSRLYEPSRTRAVVVALNISTMDQSWLGFDPLLLERNVVVAASIA
                     RWAFEERAALGLIANGSFPDADRAIRIGAGSRSDQLVRVL"
BASE COUNT          222 a          695 c          528 g          292 t
ORIGIN      
        1 ccactcgatc ggggcgacct tccaccgcat ccagttcacg cccgacctga tgccgtccga
       61 catcctcggc atcaactact actcgcagcg cacgggcgac ttcgagttcc gtcccgggcc
      121 gatcctcgcg aacgtgatcc tcgccgacga ggtgaaccgc gccaccccgc gcacccagtc
      181 cgcgctcctc gaggcgatgg aggagcgcac ggtgaccgtc gacggcgaga cgcggccgct
      241 tccggacccg ttcctcgtcc tcgccaccca gaacccgatc gaactcgaag gcacgttccc
      301 gctccctgag gcgcagctcg accggttcct gcttcgcctc aaggtcggct acccgaccga
      361 ggctggcgaa atcgaggtgc tccgccgctt cgagggtgct tcaccgctcg acgcgctgcg
      421 tccggccgtg gaggctgagg agctgctgcg cctgcgtcag gtcctcccgg gcatccacgt
      481 cgaggacgcc gttgccgcct acgtcgtgcg cctctgccgt gccacccgtg accacatcgc
      541 cttcgacctc ggtgcgagtc cacgcgcctc gctcgcgctc tttcgcgccg ctcgcgcgct
      601 cgctgccatg gacggccgtg actacgtccg ccccgacgac gtcaagcgcc tcgcgcagcc
      661 cgtcctcgcg caccgtctcg tgctctcctc gcagacccgc ctccgtggac gcagcgcgga
      721 cgatgtcctc gacgagatcc tgcacgaggt gcccgtcccc gtcgccgact agccggggac
      781 cgctgccgat gcgctctgtc ttccgcgact cttgggtcag cgtcacgatc gtcctcgtcc
      841 tggtcggcct cgccgcgcag tccgtgccac tgatcggcct gggcgtgctt gtcttcggca
      901 ccggcggcat ggcgcgcctc tgggcgcgcg tatccctcga ggacgtccgc taccggcgcg
      961 tgctccgcga acggcgcgtc ttcatgggcg agacgattga cctccgcctg cggctcgcca
     1021 acgagaagcg gctgcccgtc ccctggctcg aggtccgcga gtacctgccc gaggccatgc
     1081 ccgccggcgg agtccacgtc acaccctccg gcctccccgg caccgtgctc atgcagcgct
     1141 cgacgtcgct cggggcgcac gagcagctcg aatggccgct cacgctcgat gccaccgaac
     1201 gcggcttctt ccgcctcggc cccacgcgtc tgcgctccgg cgacctcttc ggcatcttcg
     1261 agcgcgaaga gaccatccgc ggcggcgaca gcgtcgtcgt ctacccgcgc acctattcgt
     1321 tgcccgagct gggcatcatc agcgcccgcc ccttcggcga gcgcgccggt ggccggcgca
     1381 tcttcgagga tccagtccgt gtggtcggcg tgcgtgacta cttcccgggc gaccccctca
     1441 agcgagttga ctggaacgcc acggcacgta tcggccgcct gcagtcgagg ctctacgagc
     1501 cgtcacgcac ccgggccgtc gtcgtcgcgc tcaacatcag caccatggat cagtcgtggc
     1561 tcggcttcga cccgctcctc ctcgaacgca atgtcgttgt tgccgcctcc atcgcgcgct
     1621 gggccttcga ggagcgcgcg gccctcgggc tcatcgccaa tggctcgttc cccgacgccg
     1681 accgcgcgat ccgcatcggc gccggcagcc gctccgacca gctcgtccgc gtcctcg
//
LOCUS       DAORPG010000461         1734 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9130_length_1734_cov_3.034544, whole genome shotgun sequence.
ACCESSION   DAORPG010000461 DAORPG010000000
VERSION     DAORPG010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9130_length_1734_cov_3.034544"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..>1734
                     /gene="kdpB"
                     /locus_tag="PLX85_09205"
     CDS             104..>1734
                     /gene="kdpB"
                     /locus_tag="PLX85_09205"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HRC63382.1"
                     /translation="MAVAQAPAPVPLRVEKRQTASVWTAQIVRQAVVDSFRKLAPWKL
                     YKNVIIFIVEVGALLTTLICIRDVIDGKSFELARFELQISVWLWFTVLFANFAEAIAE
                     GRGKAQADTLRRTRTQSTARRVRDDGIEDVPASLLRRGDVVVVASGELIPGDGDVIEG
                     VAYVNEAAITGESAPVVKEPGTDIRSSVTGGTTVVSDSLRIRITANPGETFLDRMIAL
                     VEGAERQKTPNEVALNILLSGLTFIFLMVTVTLQPFARYSGSEISVVILVALLVTLIP
                     TTIGGLLSAIGIAGMDRLVQRNVLAMSGRAVEAAGDVDTLLLDKTGTITFGNRLASEF
                     VPVDGVQAERLVSAAVRCSLGDETPEGRSVIDLARAQGYAHLVGTLPADAELVPFSAE
                     TRLSGVRSNGTLVAKGAVDAISAAAGSDPSRDLVAHSQRIAGEGGTPLAVLDGHQVLG
                     LIYLKDTVKPGIRQRFEELRRMGIRTVMVTGDNRLTAATIARDAGVDDFVAESKPEDK
                     IRVIREEQAQGRLVAMTGDGTNDAPALAQADVGVA"
BASE COUNT          288 a          594 c          555 g          297 t
ORIGIN      
        1 gtcgggctcc tgacgttctt ccctgccttc gcgctcggcc caatcgtcga gcacctcatg
       61 cgcagcggaa cactgttcta ggcattcgag gaggacgact gagatggccg tagcgcaagc
      121 gcctgctccg gtgccactcc gagtcgagaa gcgtcagaca gcgagcgtct ggaccgcaca
      181 gatcgttcgc caggccgttg tggattcgtt ccgcaagctg gctccctgga aactctataa
      241 gaacgtcatc atcttcatcg tcgaagtggg cgcactcctg acgaccctga tctgcatcag
      301 agacgtcatc gatgggaaat cgttcgagct cgcgcgcttc gaactgcaaa tttcggtctg
      361 gctctggttc accgtgctgt tcgcgaactt cgccgaggcg atcgctgaag gccgtggcaa
      421 agcgcaggcc gacacccttc gccggactcg cacccagtcc accgcccgtc gcgtgcgaga
      481 cgacggcatc gaggacgtac cggcaagcct gctccgtcgc ggcgatgtcg tcgtcgtggc
      541 ctccggcgag ctcattccag gcgacgggga cgtcatcgag ggggtcgcct acgtgaatga
      601 ggcggcgatc acgggcgagt ccgccccggt cgtgaaggag ccgggtaccg atatccgtag
      661 ctcggtgact ggcggcacga ctgtggtcag cgactcgctc cgcatccgta tcacggccaa
      721 ccctggcgag acgttcctcg accgcatgat cgcgctggtc gagggcgccg agcgccagaa
      781 gacaccaaac gaggtggcgc tcaacatcct cctgagcggg ctcacgttca tcttcctgat
      841 ggtcacggtg accctccagc cgttcgcccg ctactccggc tccgagatat ccgtagtcat
      901 cctcgtggcc ctcttggtca ccctgatccc gaccacgatt ggcggcctgc tctccgccat
      961 tgggatcgcc ggcatggacc gcctcgtgca gcgcaacgtg ctcgcgatgt cgggtcgcgc
     1021 ggtcgaggcc gcgggcgacg tcgacacgtt gctgctcgac aagaccggca cgatcacctt
     1081 cggcaaccgc ctcgcgtccg agttcgtccc ggtcgatggc gtccaggcag aacgtctggt
     1141 ctcggcggcg gtacgttgct ccctcggcga cgagacgccc gagggccgct cggtgatcga
     1201 cctcgcccgc gcccagggtt acgcgcacct cgtaggcacg ttgccggccg acgccgaact
     1261 cgtccccttc agcgccgaga cgcggctcag tggcgtgcgg tccaacggca cactggtggc
     1321 gaaaggcgcc gtcgacgcga tctctgccgc tgccgggagc gacccgtccc gtgaccttgt
     1381 cgcccactcg cagcggattg ctggcgaagg aggcacgccc ctcgccgtac tcgacggcca
     1441 ccaggtgctt ggcctgatct acctgaagga cacagtgaag cccggcatcc ggcagcggtt
     1501 cgaggaactc cgccgcatgg gcattcgcac cgtcatggtc acgggcgaca accgcctcac
     1561 tgcggcgacc atcgcacgtg acgccggcgt cgatgacttc gtcgcggaga gcaagcccga
     1621 ggacaagatc cgcgtcatcc gggaggagca ggcgcagggc cgtttggtcg ccatgacggg
     1681 cgatgggaca aatgacgccc cggcgctggc ccaggccgac gtgggcgtcg ccat
//
LOCUS       DAORPG010000462         1731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9151_length_1731_cov_4.124702, whole genome shotgun sequence.
ACCESSION   DAORPG010000462 DAORPG010000000
VERSION     DAORPG010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9151_length_1731_cov_4.124702"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PLX85_09210"
     CDS             complement(<1..418)
                     /locus_tag="PLX85_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRC63383.1"
                     /translation="MSTPHVYDPDAAAGEREFNLESIRRDFPVLRREVNGRPLVYLDN
                     AATSQKPVQVIAEITRYYRDFNANIHRGVHTLSQEATDAYEATREKVRAFINGYHTAE
                     IVFTRNTTESINLVAHSYGRTFLQPGDEVVISELEHH"
     gene            complement(415..723)
                     /locus_tag="PLX85_09215"
     CDS             complement(415..723)
                     /locus_tag="PLX85_09215"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HRC63384.1"
                     /translation="MASAADIPAGEVRVVQCGARSLAVSNVDGTLYAIDNRCTHDNGP
                     LGEGTLRGARIICPRHGAAFDARTGRVLTLPAVRSVHSYPVTVDGDEVYIDCEGGRAP
                     "
     gene            complement(735..>1731)
                     /gene="sufD"
                     /locus_tag="PLX85_09220"
     CDS             complement(735..>1731)
                     /gene="sufD"
                     /locus_tag="PLX85_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HRC63385.1"
                     /translation="VEGTAPAGGFAGRLTGGPAEVAREHLGSVVATTEGKFIAANAAQ
                     WEDGVLVHVPRGQAFEQPVVVTIDAAGLASAAVYPRVLVVAEEQGEVTIVLRTRSGAD
                     PLLAVGVVEVIAGQAARVKLLLDDQWGEATRDFTWVRARLAKDADLQVASVAIGGQLL
                     KQTVEVLLEGEGATSVIRAVALGNADQHFDFVTLQDHIGPRTTSEVEVKSALAGASRS
                     VYYGITRVGEGAHGANADQANKNLLLSGSAKADSDPVLEILTADVIRCGHGATVGPVD
                     QEALFYLQSRGLDRRQALQLLVAGFFQSVVGELPLEGFGEQLEERVFAKLQTADL"
BASE COUNT          260 a          593 c          613 g          265 t
ORIGIN      
        1 agtggtgctc gagctccgag atcacgacct cgtcgccggg ctgcaggaac gtgcgcccgt
       61 acgagtgcgc gacgaggttg atcgactccg tcgtgttccg cgtgaagacg atctcggcag
      121 tgtggtagcc gttgatgaag gcgcgcacct tctcgcgggt cgcctcgtag gcatcggtcg
      181 cttcctgcga gagcgtgtgg acgccccggt ggatgttggc gttgaagtcg cggtagtagc
      241 gcgtgatctc ggcgatcacc tgcacgggct tctgcgacgt cgcggcgttg tcgaggtaca
      301 cgagtggacg cccgttcacc tcgcggcgca gcaccgggaa gtcacggcgg atggactcga
      361 ggttgaactc gcgctcgcct gccgcggcat ccggatcgta gacatggggc gtgctcacgg
      421 cgcacgacct ccctcgcagt cgatgtacac ctcgtcgccg tcgaccgtga ctggatagga
      481 gtggacgctg cgcacggcgg ggagcgtcag gacgcggccc gtacgagcgt cgaacgccgc
      541 gccgtgacga gggcagatga tgcgggcgcc tcggagcgtg ccctccccga gtgggccgtt
      601 gtcgtgcgtg cagcggttgt cgatggcata caacgtgccg tcgacgttgc tgaccgcgag
      661 cgagcgcgcg ccacactgca caacgcggac ctcgccggcc gggatatccg ccgcgctcgc
      721 cacgcgctcc ttcgtcacag gtccgccgtc tgcagcttcg cgaacacgcg ctcctcgagc
      781 tgctcaccga agccctcgag gggcagctcg ccgacgaccg actggaagaa gccggccacg
      841 agcagctgca gcgcctgccg gcggtcgagc ccgcggctct ggaggtagaa cagcgcttcc
      901 tggtcgaccg ggcccaccgt cgcgccatgg ccgcagcgga tgacgtccgc ggtcaggatc
      961 tcgaggaccg ggtcggagtc cgccttcgcc gagccggaca ggaggaggtt cttgttcgcc
     1021 tgatcggcgt tggcgccgtg ggcgccctcg cccacgcgcg tgatgccgta gtagacggag
     1081 cgcgaggccc cggcgagcgc cgacttcacc tcgacctccg aggtcgtgcg gggcccgatg
     1141 tgatcctgca gcgtgacgaa gtcgaagtgc tgatccgcgt tccccagcgc gaccgcgcgg
     1201 atgacggacg tcgctccctc accctcgagg agtacctcga ccgtctgctt gagcagctgt
     1261 ccgccgatag cgaccgacgc gacctgaagg tcggcgtcct tggcgaggcg tgcccgcacc
     1321 caggtgaagt cgcgcgtcgc ctcgccccac tggtcgtcga gcagcagctt cactcgtgcg
     1381 gcctggccgg cgatgacctc gacgacgccg accgcgagga ggggatccgc gcccgaacgc
     1441 gtgcgcagca cgatggtgac ctcgccctgc tcttcagcga cgacgagcac gcgcgggtac
     1501 acagcggccg aagcgagccc ggctgcgtcg atggtgacga ccacgggttg ctcgaaggcc
     1561 tgaccgcgtg gcacgtggac gaggacgccg tcctcccact gcgcggcgtt cgccgcgatg
     1621 aacttgccct cagtcgtggc aacgacggaa ccgaggtgct cccgggcgac ctcggccggg
     1681 ccgccggtaa gacggcccgc gaagccgcca gcgggggccg tgccctcgac a
//
LOCUS       DAORPG010000463         1730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9166_length_1730_cov_2.523582, whole genome shotgun sequence.
ACCESSION   DAORPG010000463 DAORPG010000000
VERSION     DAORPG010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9166_length_1730_cov_2.523582"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PLX85_09225"
     CDS             complement(<1..711)
                     /locus_tag="PLX85_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRC63386.1"
                     /translation="MSLGQPLALLALLALLPLVTAWWYTRRRSLASAAVYGGSASLRR
                     GLSPRRAQLRAGLLFAAVALVAVAIARPQWGGAEGAVTRRGIDVVIALDVSRSMTATD
                     VQPSRSNAAAAGLDDMLAHLRGDRVGLVTFGGSAFARSPLTLDLGAVSFLVERAQLEG
                     ALVEPGTDFAKAIQTSLTLLDVPDRANSQAIVLLSDGENLGSNLDAAIAAARDRGVRV
                     YTVAVGTEAGAAVPARTSR"
     gene            complement(708..1667)
                     /locus_tag="PLX85_09230"
     CDS             complement(708..1667)
                     /locus_tag="PLX85_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRC63387.1"
                     /translation="MSFGTPWLLLLLPLAALIPFWQWRRPAAHPALAVADLGVLATVA
                     RDQSSWRIRLRWLPAALRVAAIALIVVALARPQRGLAVTSIPEEGIDIVLALDVSGSM
                     AERTQPSGGGIGPPRIEAAKDVIAQFVDTLRGDRAGFVIFQGRSLVLSPLTLDLAALK
                     RTVHDTESGLLPDGTAIGLGLSEALNLLRDSEAHSRIVVLLTDGQNNAGDIEPVQAAQ
                     LAKALGVRVYTIGFTSRRGSGEVDETMLRRIASDTEGAYHDASTQQELAAAYDEIGAL
                     ERSRVAERTFTRYEEFAPWLAAAALALLISEAALRATLLRRFP"
BASE COUNT          261 a          582 c          661 g          226 t
ORIGIN      
        1 cctcgaggtg cgcgccggca cggcagctcc cgcctcggtg ccgaccgcga cggtgtagac
       61 gcggacgcca cggtcgcgag cggcggcgat cgcggcgtcg aggttggacc cgaggttctc
      121 gccgtcggag agcagcacga tcgcctggct gttcgcacgg tccggcacat cgaggagtgt
      181 cagcgacgtc tggatcgcct tcgcgaagtc cgtgccgggc tcgaccagcg cgccttcgag
      241 ctgtgcacgt tcgacgagga atgacaccgc gcccagatcg agcgtaaggg gcgagcgcgc
      301 gaaggcgctc ccgccgaagg tcacgaggcc gacgcggtca cctcgcaggt gggccagcat
      361 gtcgtcgagg cccgctgcgg cagcgttcga gcgcgacggc tgcacatccg tcgcggtcat
      421 cgatcgcgac acgtcgagcg cgatgacgac gtcaatgccg cgacgggtga cggcgccctc
      481 ggcgcctccc cattgcgggc gcgcgatcgc gactgcgacc agggcgacgg ccgcgaagag
      541 cagccccgcc cggagctgcg cgcgccgtgg gctcaggccg cgccgcagcg aggcagatcc
      601 gccatacacg gcggcgctgg cgaggcttct gcggcgcgtg taccaccagg cggttacgag
      661 gggcagcaac gcgagcagcg ccagcagtgc gagcggctgt ccgaggctca cgggaacctc
      721 cgcagcagcg tggcccgcag cgccgcctcg ctgatcagca gcgcgagcgc ggcggcggcc
      781 agccagggtg cgaactcctc gtagcgggtg aacgtgcgct cggcgacgcg gctccgttcg
      841 agcgcgccga tctcgtcgta cgccgcggcc agctcctgct gcgtcgatgc gtcgtggtag
      901 gcgccttcgg tgtcggaggc gatacggcgg agcatcgtct cgtcgacttc gccggagcct
      961 cgacggctgg tgaagccgat cgtgtagacg cgcacgccga gcgctttcgc gagctgcgcc
     1021 gcctgcacgg gctcgatgtc ccccgcgttg ttctggccgt ccgtcagcag cacgacgatg
     1081 cggctgtgcg cctcggagtc gcgcaggagg ttgagcgcct cggagagccc gagtccgatg
     1141 gccgtaccgt ccggcagcag accgctctcg gtgtcgtgga ctgtgcgttt gagcgcagcg
     1201 aggtcgagtg tgagcggcga gagcacgagc gagcggccct ggaagatgac aaacccagcc
     1261 cggtcgccgc gcagcgtgtc gacgaactgg gcgatcacgt ccttggcggc ctcgatgcgc
     1321 ggcgggccga tgccgccgcc tgagggctgg gtgcgctccg ccatcgagcc ggagacgtcg
     1381 agtgcgagga cgatgtcaat gccctcctcg gggatggacg tgactgccag gccgcgctgc
     1441 ggacgggcga gtgcgacgac gatcaacgcg atggcggcga cgcggagtgc ggcaggcaac
     1501 cagcgcagcc ggatgcgcca gctcgactgg tcccgggcca ccgtcgcgag cacgccgagg
     1561 tcggcgaccg ccagcgcggg atgagctgcg gggcggcgcc actgccagaa cgggatcagc
     1621 gccgcgagcg gcaggagcaa caggagccag ggcgtaccga agctcacggg agtgcctcgg
     1681 cagaggccgg ctcggcgttc tgggaatgat ccggggggcg ctggatcacc
//
LOCUS       DAORPG010000464         1728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9177_length_1728_cov_3.371787, whole genome shotgun sequence.
ACCESSION   DAORPG010000464 DAORPG010000000
VERSION     DAORPG010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9177_length_1728_cov_3.371787"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PLX85_09235"
     CDS             <1..355
                     /locus_tag="PLX85_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRC63388.1"
                     /translation="RGDDIAARIRAVAPEGVDGLADGAVQQELAIGAVRDGGGFASVR
                     GWRGNEERGITFHRTWVGSYDHRPDLLDRLRQQVEDGQLTLRVAATYPAEQAAEAHRR
                     FQAGGTRGRLVIRFD"
     gene            478..600
                     /locus_tag="PLX85_09240"
                     /pseudo
     CDS             478..600
                     /locus_tag="PLX85_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            755..1198
                     /locus_tag="PLX85_09245"
     CDS             755..1198
                     /locus_tag="PLX85_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HRC63389.1"
                     /translation="MVSEHHVTAHAEHAGAFGSGGFGAFAEAIARFFGTPRYLAAQTA
                     LVALWIAANGWALLHFDPYPFILLNLAFSLQAAYAAPLILLAQTRQAARDKAFSEADS
                     RHREQLTQQQLQLLLENTRLTEEVHRLTAEVHALVERIPLPSAAS"
     gene            1512..>1728
                     /locus_tag="PLX85_09250"
     CDS             1512..>1728
                     /locus_tag="PLX85_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63390.1"
                     /translation="MRIELTARGIFFILLAIAGIIVLRRLWPVVILVVTALIFMGALL
                     PYVEWLVARGVNRGVAVLLVAFAVFAAI"
BASE COUNT          245 a          730 c          490 g          263 t
ORIGIN      
        1 ccgcggcgac gacatcgccg ctcgcatccg cgcagtcgct cccgagggtg tcgacggcct
       61 cgccgatggc gcggtgcagc aggagctcgc catcggggcc gtccgcgacg gcggcggctt
      121 cgcctccgtc cgtggctggc gcggcaacga ggagcgtggc atcacgttcc accgcacgtg
      181 ggtcggctcc tacgaccacc gccccgacct cctcgatcgc ctgcgccagc aggtcgagga
      241 cggccagctc accctccgcg tcgccgccac ctacccggcc gaacaggccg ccgaggccca
      301 ccgccgtttc caggccggcg gcacccgcgg ccgcctcgtc atccgcttcg actgagcccg
      361 tccatcttcg gcgcgtgcgg atgttgtatg gatcagcgcc cactacatga tgaagtggag
      421 attgagtaat aagatcgccc tgacgaaacc gcgcatcgac ggatcggggt cacgccaatg
      481 atcggcgaca tcggctggga agaactcctg atcatcctcg tcatcctcgc gctcgtcttc
      541 ggcgcctccc gcgtcgccga cctcggcggc gcgctcggtc gcggcatccg cgagttccgc
      601 cacgaagccg gcgcaaagcc cgacaccgac gatcacccgt cctctggcga tgccatccct
      661 cccgcggaga cccccgcccc gccgcccgcg aactgagcat cgtccggagg cacagacccc
      721 gagccacacc ccggggcctc gaggaggtcg cgcgatggtc agcgagcacc acgtcacagc
      781 ccacgccgag cacgccggcg ccttcggttc cggcggcttc ggcgccttcg ccgaggccat
      841 cgctcgcttc ttcggcacgc cgcgctacct ggccgcgcag accgccctcg tggccctctg
      901 gatcgccgcc aatggctggg cgctcctcca cttcgacccc taccccttca tcctcctcaa
      961 cctcgccttc tccctgcagg ccgcctacgc cgcgccgctc atcctcctcg cgcagacacg
     1021 acaggccgcc cgcgacaaag ccttctccga ggccgactca cgccaccgcg aacagctcac
     1081 gcaacaacag ctccagctgc tcctcgaaaa cacccgcctc accgaggaag ttcaccgcct
     1141 caccgccgaa gtccacgccc tcgtcgaacg catccccttg cccagcgccg ccagttgacc
     1201 ccctccttgg gggactgacg aggcgtcgtc ggtcccccgg cgctgacgac accgccgccc
     1261 ccagacccgc cgcccaccgg ccgtaagctg gtcgccgagc tcggccacac gcccgctccc
     1321 gcctcccttt cgcccgcgcc cggcatctcg cggatccatc gcgtgcgccc gtccgagcga
     1381 gcacgggaga cttgggagga ctccccgtcc tcctccttgg gagtctgcta tcctgcccga
     1441 atcaaccgcc gccccaacgc ggcggatgcc ccgccgggcg ggcggcgagg agaggcggca
     1501 caccaccctc cgtgaggatc gaactcaccg cccgcggcat cttcttcatc ctgctggcca
     1561 tcgccggcat catcgtcctg cgccgcctct ggcccgtcgt catccttgtc gtcaccgccc
     1621 tcatcttcat gggcgccctg ctcccctacg tcgaatggct cgtcgctcgc ggcgtcaatc
     1681 gaggcgtcgc cgtcctcctc gttgccttcg ccgtcttcgc cgccatcg
//
LOCUS       DAORPG010000465         1723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9221_length_1723_cov_3.478417, whole genome shotgun sequence.
ACCESSION   DAORPG010000465 DAORPG010000000
VERSION     DAORPG010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9221_length_1723_cov_3.478417"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PLX85_09255"
     CDS             complement(<1..712)
                     /locus_tag="PLX85_09255"
                     /inference="COORDINATES: protein motif:HMM:NF023495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HRC63391.1"
                     /translation="MDALSLAIVVISFFVGVGSTLLAARVGARGLITDAEERAARIRE
                     EAENSRRAIEIEAQEDAIKYRRQVEDEVREQRREVGRIERRLEQREEALERRSEGLDR
                     GEQSLRDRMAQLDRTEQDLSEARAAADTELERIAGMSVEEARNALLERLDTELRDEAA
                     RRVRSMETSAKAEADVRARKVLATVMQRMTSEITAETTVSVVPIPSDEIKGRIIGREG
                     RNIRAFEAATGCDLIIDDT"
     gene            complement(946..>1723)
                     /gene="recA"
                     /locus_tag="PLX85_09260"
     CDS             complement(946..>1723)
                     /gene="recA"
                     /locus_tag="PLX85_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020615211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRC63392.1"
                     /translation="DIDELLVSQPDTGEQALEICEALVRSNAVDIVVVDSVAALVPRA
                     EIEGDMGDSLPGIQARLMSQALRKLTGAVARSNTALVFINQLREKVGVVFGNPEVTPG
                     GRALKFYSSVRIEIRRVESLKQGTTIIGNRVRCKVVKNKTAPPFRQAEFDIMFTAEEH
                     GISREGDILDLAVQHDVVKKLGAFYSYGEQRIGQGREAAKAFLREHQAMAGEIEVRVR
                     TAAGVGASASAAAPAAVEDEAESEDLEFVSAGGSELPLDL"
BASE COUNT          263 a          597 c          541 g          322 t
ORIGIN      
        1 gagtgtcgtc gatgatgaga tcgcaccctg ttgcggcctc gaaggcgcgg atattgcggc
       61 cctcgcggcc gatgatgcgg cccttgattt cgtctgacgg gatcggcacg accgagacgg
      121 tcgtctccgc cgtgatttcc gaggtcatgc gctgcatcac cgtcgcgagc accttgcgcg
      181 cccgcacgtc cgcttccgcc ttcgccgagg tctccatgga gcgcacgcgc cgcgccgcct
      241 cgtcccgcag ctcggtatcg agccgttcga ggagtgcgtt gcgcgcctcc tcgacgctca
      301 tcccggcaat ccgctcgagt tccgtgtccg cggccgctcg tgcctcagac aggtcttgct
      361 cggtccggtc gagttgcgcc atccggtcac gcagcgactg ctcaccacga tcgaggccct
      421 cggaacgccg ttcgagggcc tcttcacgct gctccagccg ccgctcgatg cggccgactt
      481 ctcggcgttg ttcgcggact tcatcctcga cctgccgccg gtacttgatg gcgtcttcct
      541 gcgcctcgat ctcgatggcg cggcggctgt tttccgcctc ttcgcggatc cgcgccgcgc
      601 gctcctcggc atcggtgatc agtccgcgtg cgccgacgcg ggccgcaagc aacgtgctcc
      661 ccactcctac gaagaaggag atcacgacaa tcgcaaggga aagggcgtcc atgaggctcc
      721 ggtcctaagg gaagcgtcgc gtcttccgcg ccgcctgtaa cggtgacgta gagatatgtc
      781 tacgtccgta tcgtagcacc ggcgcttgga gggcgggagt gcgggcgaga ttcggtgaat
      841 acaaatgcgc accgcggcgc tcaatagcac ctatcgaggt gtcagcgtgt tcccccggta
      901 ctggtgggag ggcgtgagca tcgccctccc actcacgctt gcgtgctaga ggtcgagtgg
      961 cagctccgag ccacccgccg agacgaattc gaggtcttcg gactctgctt catcctcgac
     1021 ggcggcaggt gctgctgccg aggccgaggc gcccacgccc gccgcggttc gcacccgtac
     1081 ctcgatctca cccgccatcg cctggtgctc ccgcaagaac gccttggcgg cctcgcggcc
     1141 ctgcccgatg cgctgctcgc catacgagta gaacgcgccc aacttcttta cgacgtcgtg
     1201 ctgcacggcg agatcgagaa tgtcgccctc gcggctgatg ccgtgctctt ccgccgtgaa
     1261 catgatgtcg aactccgcct ggcggaacgg gggagcagtc ttgttcttca cgaccttgca
     1321 gcgaacacgg ttgccgatga tggtcgtgcc ctgcttcagc gattcgacgc gccgaatctc
     1381 gatccggacc gaggagtaga acttcagtgc gcgcccgccc ggcgtcacct cgggattacc
     1441 gaagaccacg ccgacctttt cacgcagctg gttgatgaac acgagcgccg tattcgagcg
     1501 cgcgacggcg cccgtgagct ttcggagggc ctgcgacatc agccgcgcct ggatgccagg
     1561 cagcgagtcg cccatgtcgc cttcgatctc cgcacgcggc acgagggcgg cgaccgagtc
     1621 gacgacgacg atgtcgacgg cgtttgaacg cacgagcgcc tcgcagatct cgagcgcctg
     1681 ctcgccggtg tccggctgcg acacaagcaa ctcgtcgatg tcg
//
LOCUS       DAORPG010000466         1720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9243_length_1720_cov_3.825225, whole genome shotgun sequence.
ACCESSION   DAORPG010000466 DAORPG010000000
VERSION     DAORPG010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9243_length_1720_cov_3.825225"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLX85_09265"
     CDS             <1..447
                     /locus_tag="PLX85_09265"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRC63393.1"
                     /translation="GGAALVSRLTESLFVEVIQRYVRAQGSEGWFAALRDPVAGPALA
                     LIHAASARHWSVEELASEVGVSRTVLADRFTATVGQSPIRYLTSWRLEVAGQRLLDTH
                     DSVAEVALAVGYESEAAFNRAFKRFTGQPPAAWRRAQRTASSSLAG"
     gene            complement(451..1379)
                     /locus_tag="PLX85_09270"
                     /pseudo
     CDS             complement(451..1379)
                     /locus_tag="PLX85_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004955055.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
     gene            complement(1483..>1720)
                     /locus_tag="PLX85_09275"
     CDS             complement(1483..>1720)
                     /locus_tag="PLX85_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC63394.1"
                     /translation="RSDVAEHLEPAAARVEALTPREVEILALLAGGSANKEIAARLGL
                     SVHTVERHLTNLYGKIGVRSRSEATAYALTHDYR"
BASE COUNT          239 a          641 c          576 g          264 t
ORIGIN      
        1 gggggagccg cccttgtgag ccggctcacg gagtcgttgt tcgtcgaggt gatccagcgc
       61 tacgtccgcg cccagggcag cgagggctgg tttgctgccc tgcgtgaccc ggtcgccggg
      121 cccgcgctgg cgctcatcca cgccgcatcg gcccggcact ggtcggtcga agagctggcg
      181 tccgaagtcg gcgtgtcgag gacagtgctg gccgaccgct tcacggcgac tgtgggccag
      241 tcgccgatcc ggtacctgac atcctggcgc ctcgaagtgg ccggtcaacg cctgctcgac
      301 acccacgact cggtcgccga ggtcgcgctg gccgtcggct acgagtcaga agccgcgttc
      361 aaccgcgctt tcaagcgctt cacgggccag cccccggccg cctggcggcg ggctcagcgc
      421 accgcctcga gttcgctcgc tggctaacgg tcatgccttc gaggcagccg ccgaccgccc
      481 gcctgcccga ggcccgagca ccgacgccag cgcatcttcg accgtctcgt gctcgaatcg
      541 gaaccccgag cgcgtgagtt tcgagggcaa cacgcgctgg ctctgcagca acagctcatt
      601 cgccatttcg ccgagcgcga tgcggagcac gaatgccggc accgggaaca gcgccggccg
      661 gtgcagctgc cgcgcgagtg ccttcgtgag ttcgcggttg gtcaccggat gcggcgccgc
      721 gaggttcacc ggtccgcgca cttcctcgtg cgtgatcgcc cactcgatgg cgcgcacttc
      781 gtcttcgagc gtgatccacg gcatccactg ccgcccgctc cccagccgac cgcccacgcc
      841 aaagcggaag ggcgtcagca tgcgctgcaa cgccccgcct cgaggcgcga gcacgaccgc
      901 cgtccgaatc gacaggacgc ggattccggc ctcggacgcg cggaccgcct cgcgctccca
      961 gtcgcggacc acccccgcga ggaagccctc tcccgcccgg gactcctcgc gcagctcctc
     1021 gtcgccccgg tcgccgtagt acccgaccgc cgatccgcag acgaacacac gcggcggtcg
     1081 cgcgaatgcc gtcatgtggt cgacaagcag acgtgtcgat tcgatccggc tgtctcgaat
     1141 cgcccgcttc cgccgcgcgg tccagcgtcc gtcgccgatg gactcccccg cgaggtgcac
     1201 gacggcgtcg cacccttcga gcgccccgtc ctcgatccag cctcggctgg ccgccaggca
     1261 acggcgccga gcgtcggggt tcctcgccgg acggacacca cctcgtggcc cccgcgttcg
     1321 aggcgcgccc gcagcgccgt cccgatgagc cctgaactgc ctgtgattgc gacccgcatc
     1381 gcccgctccg atccctacgc tcctgacggt aggagcgcgt tgagggcggc gcgtcgtgga
     1441 aacctcgtac gtggagcggc ggaaatctcg tagggagagg ggctaccggt agtcgtgggt
     1501 gagggcgtag gcagtggcct cggagcggct gcgcactccg atcttgccgt agaggttggt
     1561 caggtggcgc tcgaccgtgt gcacgctgag cccgaggcgt gccgcgatct ccttgttggc
     1621 gctgcctcct gccagcagcg cgaggatctc cacttcgcga ggagtgagcg cctcaacgcg
     1681 tgcggccgcc ggctccaggt gctcggcgac gtccgaacgc
//
LOCUS       DAORPG010000467         1720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9247_length_1720_cov_3.166366, whole genome shotgun sequence.
ACCESSION   DAORPG010000467 DAORPG010000000
VERSION     DAORPG010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9247_length_1720_cov_3.166366"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PLX85_09280"
     CDS             complement(<1..486)
                     /locus_tag="PLX85_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRC63395.1"
                     /translation="MAAEATPESDEPLTDALLEEIEAVAVELAHLAGAEIDSALGREI
                     VVEYKSASKKGNVEPTNPVSEVDHAVEALVRERLALRFPGHGIIGEEVELQPDPTRDF
                     VWVIDPVDGTTNFVNGFPLFAASIGVLHRGVPVAGAIWCSTGHELRSGVYHARAGGPL
                     RF"
     gene            539..721
                     /locus_tag="PLX85_09285"
     CDS             539..721
                     /locus_tag="PLX85_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63396.1"
                     /translation="MADDDWVTSTEGDLDPDLTEEAGYSGWDPPDRSGWALVQRLVMA
                     LVLVSIVGGALLILAR"
     gene            908..1651
                     /locus_tag="PLX85_09290"
     CDS             908..1651
                     /locus_tag="PLX85_09290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRC63397.1"
                     /translation="MADGLLLLHAFPLDASMWEPQVAAFKDRLPVAAPDFPGLGGGSD
                     APEVMTMDAAADAGIRAMDAAGIEKAVVCGLSMGGYAALAFWRNHRDRVAGLVLANTR
                     SGADDEAGKERRKALADRLKAEGSQFLVDGPPPLLSGEASEELWGRVKGIIAKQSAAG
                     IAGASLGMGARPDYTDDLAGIDVPVLVITSTGDTLIPADATKPMASQIPNAQLEVIEG
                     AGHLTNIEATEEFNRLLGTHLVKCGVLAG"
BASE COUNT          265 a          557 c          643 g          255 t
ORIGIN      
        1 gaagcggagc gggccgccgg cgcgggcgtg ataaacgccg gaacgcagct cgtggccggt
       61 ggagcaccag atggcgccgg cgaccgggac gccccgatgg aggacgccga tcgaggcggc
      121 gaacagcggg aatccgttca cgaagttggt ggtgccgtcg acggggtcga tcacccacac
      181 gaaatcgcgc gtggggtcgg gctgcagctc gacttcctcg ccgatgatgc cgtggccggg
      241 aaagcgcagc gcaaggcgtt cgcggacgag cgcttcgacg gcgtggtcga cctccgagac
      301 ggggttcgtg ggctcgacgt tgcccttctt gctggcgctc ttgtactcga ccacgatctc
      361 gcggccgagc gcgctgtcga tttcggcgcc cgcgaggtgc gcgagctcga ccgcgaccgc
      421 ctcgatctcc tcgaggagcg cgtcggtgag cggttcgtcg gactcggggg ttgcctcggc
      481 agccatcgag gtcagcatac cgcgtgaccc gccgccatcc gttgcgtagc ctgtctgtat
      541 ggcagacgac gactgggtga cctcgacgga gggtgacctc gacccggacc tgacggagga
      601 agccggttac tccggctggg acccgccgga ccgcagcggc tgggcgctgg tacagcgcct
      661 ggtgatggcg ctcgtgctcg tgagcatcgt gggtggagcg ctgctcatcc tcgccaggta
      721 ggcttcgcgc gccgctcacg cgagccgaca ggctgccggc ccggcaagcg tcgtgcttgc
      781 gcgatcggcg cgcgggcggc atcgtcgagc agtggtcgta cgtagttcgc aagtacgtgc
      841 gggctatcga gcagacgacc gcaatacgaa ctaccccagt ctgattgagc gtgaggagag
      901 taccgagatg gccgacggac tgttgttgct acacgccttt cccctggacg cctcgatgtg
      961 ggagccacag gtcgcggcgt tcaaggaccg cctgccggtg gcggcgcccg atttcccggg
     1021 cctgggcggc ggcagcgacg cacccgaggt gatgacgatg gacgcggcgg ccgacgcggg
     1081 gatccgcgcg atggacgcgg ccggcatcga gaaggccgtg gtctgcgggc tctccatggg
     1141 cggctacgcg gcactcgcat tctggcgcaa ccaccgcgat cgggtcgccg gcctcgtgct
     1201 ggcgaacacg cgctcgggcg ccgacgacga ggcggggaag gagcgccgca aggcgctggc
     1261 ggaccgactg aaggccgagg gatcgcagtt cctcgtcgac gggccaccgc cgctgctctc
     1321 gggcgaggcg tccgaggagc tgtggggacg ggtaaagggg atcatcgcga agcagtccgc
     1381 ggcgggcatt gccggcgcat cgctggggat gggcgcccgc ccggactaca ccgacgacct
     1441 cgcgggcatc gacgtgccgg tgctcgtcat cacctcgacg ggcgacacgc tcatcccggc
     1501 cgacgccacg aagccgatgg cctcgcaaat cccgaacgcg cagctcgagg tgatcgaagg
     1561 cgcgggtcac ctcacgaaca tcgaagcgac cgaggagttc aaccgcctgc tgggcacgca
     1621 cctcgtgaag tgcggggtgc tcgcggggta gcggtccgct tatccatgtg cgatgcgagg
     1681 ggcggcgatt gccgcccctc gtcgcgtccg agccgctagc
//
LOCUS       DAORPG010000468         1709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9341_length_1709_cov_2.276300, whole genome shotgun sequence.
ACCESSION   DAORPG010000468 DAORPG010000000
VERSION     DAORPG010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9341_length_1709_cov_2.276300"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PLX85_09295"
     CDS             complement(<1..576)
                     /locus_tag="PLX85_09295"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HRC63398.1"
                     /translation="MAMLAMVQAAAQAVTVGKNLIASLTTDVNGDPLPPEVEVAPGVT
                     VQDVLDAAAKGHAAAGAFHDRRVVRDRLHELRGALSRELLPLFAHVVEGFRREFGPAL
                     VFPSDEFYITAGLKPPRAADYEGFAQYENGIGMVRVLQDDASVTLRRVRSGRISAPRV
                     KRATLACGRLIGSELARLAGEVGDAAGVRLDV"
     gene            complement(587..1300)
                     /locus_tag="PLX85_09300"
     CDS             complement(587..1300)
                     /locus_tag="PLX85_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63399.1"
                     /translation="MNSQVRTWIRNIVATVINGAASGVVLIIADPKDFNLAEPQKLLM
                     TSAVFALFGLANFLKQHPLPEESVEVKTTITHTGGNPPLPAVLLLAGALAFAPACSTG
                     GIHSAPITANPTATQVEQTRKDVIRAIEATTSALKIANIAVASADAINKANPAIISND
                     VLRTIATAGKAFADGADIGIRELEAGGSNASMVATAKRLTQLLDPFLLSLEQSKNEKL
                     SAFAASLRIGMTVVRTIGG"
     gene            complement(1325..>1709)
                     /locus_tag="PLX85_09305"
     CDS             complement(1325..>1709)
                     /locus_tag="PLX85_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63400.1"
                     /translation="YKLTGFTAVDTTDTVTLAGMSLDSRQTLAAPGSAVVRGATAAGV
                     ANGMTGGTLTKSAVPFGRAPLWQLAAIPTAGPVPETWLESLGDGPMMLAPDEGVGVEN
                     QIAFGAAGVAQFHVLAQWDEVEVLA"
BASE COUNT          255 a          589 c          590 g          275 t
ORIGIN      
        1 aacgtcgagc cggacccccg ccgcatcgcc gacctcgccc gccaatcgcg ccaactccga
       61 accgatgagc cggccacagg cgagcgtggc tcgcttcaca cggggcgcgc tgattcgccc
      121 agaacgtacg cggcgaagcg tgaccgaggc gtcgtcctgc agcacgcgaa ccatgccgat
      181 accgttctcg tattgcgcga aaccctcgta gtcggccgca cggggaggtt tgaggccggc
      241 cgtgatgtag aactcgtcgg acgggaagac gagcgcgggg ccgaactcgc gccggaagcc
      301 ttctacgacg tgcgcgaaca gcggcaggag ctcgcgcgag agagcgccgc gcagctcgtg
      361 caggcgatct cgcaccacgc ggcgatcatg gaacgcgccg gcggccgcgt ggcccttggc
      421 tgctgcgtcg agcacgtctt gcaccgtcac gcctggtgcg acctcgacct cgggcggcaa
      481 cgggtcgccg ttcacgtcgg tcgtcagact cgcgatcaga ttcttgccga cggtcaccgc
      541 ctgcgcggcc gcctgcacca ttgccaacat cgccatcacg cccacgtcac cctccgatcg
      601 ttcgtaccac cgtcatgccg atccgcagcg acgctgcgaa cgcggacagc ttctcgttct
      661 tcgactgctc cagcgacaac aggaacgggt ccaggagctg cgtcaggcgc ttggccgttg
      721 ccaccatgct cgcgttcgag ccgccggcct ccagctcgcg gatgccgatg tcggcgccgt
      781 cggcgaacgc cttgcccgcc gtggcgatcg ttcgcagcac gtcgttcgag atgatcgccg
      841 ggttggcctt gttgatggcg tcggcgctcg cgaccgcgat gttcgcgatc ttcagcgccg
      901 aggtcgtggc ctcgatcgcg cggatgacat ccttgcgcgt ctgctcgacc tgcgtggccg
      961 tcgggttcgc ggtgatgggc gcggagtgaa tgccgcccgt cgagcaggcc ggggcaaacg
     1021 ccagggcgcc ggcgagcagc agcaccgccg gcagcggcgg gttgccgccc gtgtgtgtga
     1081 tcgtcgtctt gacctcgacc gactcctcag gcagcgggtg ctgcttcagg aagttggcga
     1141 gcccgaagag agcgaacacc gcgctcgtca tcagcagctt ctgcggttcc gccaggttga
     1201 agtccttggg gtccgcgatg atgagcacga cgccagaggc ggcgccgttg atgaccgtcg
     1261 cgacgatgtt gcggatccag gtgcgtactt ggctgttcat gggctcctcg ggggatgggc
     1321 gattctacgc caagacttcg acttcgtccc actgcgccag gacgtggaac tgcgcgacgc
     1381 cggccgcgcc gaacgcgatc tggttctcga cgccgacacc ctcatcgggc gcgagcatca
     1441 tcgggccgtc gccgagcgat tcgagccagg tctcgggcac cgggccagcg gtggggatgg
     1501 cggcgagctg ccacagcggc gcgcgtccga acggcaccgc gctcttcgtg agcgtgccgc
     1561 ccgtcatgcc gttggcgacg ccggccgcgg tggcgccccg cacgacagcc gagcccggcg
     1621 ccgcgagggt ctgccgtgag tccagcgaca tgcccgcgag cgtgaccgtg tcggtggtgt
     1681 cgaccgcggt gaagcccgtg agcttgtag
//
LOCUS       DAORPG010000469         1708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9349_length_1708_cov_3.982456, whole genome shotgun sequence.
ACCESSION   DAORPG010000469 DAORPG010000000
VERSION     DAORPG010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9349_length_1708_cov_3.982456"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="PLX85_09310"
     CDS             complement(<1..796)
                     /locus_tag="PLX85_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRC63401.1"
                     /translation="MTTEPRRRGRTRASSGKSLVIVESPAKARTIGRFLGPEYVVESS
                     IGHIRDLPSRAAEIPEKYRREAWARLGVDVDHDFEPLYIVPTTKKQQVTKLKQLLKDA
                     DQLYLATDEDREGEAIAWHLREVLNPKVPVKRMVFHEITRDAIEDAIEHPRDIDQHLV
                     EAQEARRILDRLYGYEVSPVLWKKVRPRLSAGRVQSAAIRIVVARERERMRFVEASFW
                     DVDATMRTRDDGADVSTHLVELDARRVASGRDFEPTTGRLADDRNVV"
     gene            complement(786..>1708)
                     /gene="dprA"
                     /locus_tag="PLX85_09315"
     CDS             complement(786..>1708)
                     /gene="dprA"
                     /locus_tag="PLX85_09315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRC63402.1"
                     /translation="LAGVDALAPDDPRYPSRLHEIADAPPLLYVKGSWIPDDEWSVAV
                     VGTRRATAYGRQAATEFARGLAANRVTVVSGLARGVDTVAHHTALESGGRTVAVLANG
                     LDTVYPPENARLADQIAAQGALMTDYPLGTKPRADFFPRRNRIMSGVALGTLVVEGEP
                     TSGAMITARLALEQNREVFAVPGSIFSTQSSGPLALLRDGATPVGRIEDILEALNLTM
                     VGAQMDFGRALPPDDPNEVALMASLSREPRHVDEVVRQSGLPAATVAATLALLELKGL
                     VRDMGGMQYVRLREDVAEYVVPRAGATVDH"
BASE COUNT          247 a          564 c          608 g          289 t
ORIGIN      
        1 ggacgacgtt gcggtcgtcc gcgagccgcc cggtggtcgg ctcgaagtcg cgccccgatg
       61 cgacccgccg cgcgtctagc tcaacgaggt gagttgagac gtccgctccg tcatcgcggg
      121 tgcgcatggt ggcgtcaacg tcccagaacg aggcctcgac gaagcgcatg cgctcgcgct
      181 cgcgggccac aacgatgcgg atcgccgccg actgcacgcg gccggccgag aggcgcgggc
      241 ggactttctt ccagaggacc ggtgacacct cgtagccgta caggcggtcg aggatacgcc
      301 gcgcctcctg cgcctcgaca agatgctggt cgatgtcgcg tgggtgctcg atggcgtctt
      361 cgatggcgtc ccgggtgatc tcgtggaaga ccatgcgctt cacggggacc ttggggttga
      421 gcacctcgcg gaggtgccag gcgatggctt ccccttcgcg gtcttcatct gtggcgaggt
      481 agagctggtc ggcgtccttc agcagctgct tgagcttcgt gacctgctgc ttcttcgtcg
      541 tcgggacgat gtagagcggt tcgaagtcgt ggtcgacgtc cacgccgagg cgcgcccatg
      601 cctcgcggcg gtacttctcg ggtatctcgg cggcccggga cggcaggtcg cggatgtgcc
      661 cgatcgagga ctcgacgacg tactcggggc cgaggaaccg cccgatcgtg cgcgccttgg
      721 cgggcgactc gacgatcacg agactctttc cgctgctggc gcgtgtccgg cccctccgcc
      781 ggggttcagt ggtcaaccgt tgctcctgcc ctcgggacga cgtactcggc gacgtcctcg
      841 cgcagtcgaa cgtactgcat gccgcccatg tcgcgcacga ggcccttcaa ttcgaggagc
      901 gcgagcgtgg ctgccacggt ggcggccggg agcccgctct ggcgcacgac ctcgtccacg
      961 tgacgtggct ctcgggagag ggaggccatc agcgcgacct cgttcgggtc gtccggtggc
     1021 agcgcgcgac cgaagtccat ctgggcgccg accatcgtga ggttcagcgc ctcgaggatg
     1081 tcctcgatac gcccgaccgg cgtggcgccg tcccggagca gcgccagggg accgctcgat
     1141 tgggtagaaa aaatcgagcc gggcacagcg aacacttcgc ggttctgctc gagggcaaga
     1201 cgcgccgtga tcatcgcgcc gcttgtcggt tcgccctcca ccaccagtgt gcccagagcc
     1261 actccggaca taatgcggtt tcgacgcggg aagaagtccg ctcggggttt cgtcccgagc
     1321 gggtaatcgg tcatcagcgc tccctgggcc gcgatctggt cggcaagacg cgcgttctcg
     1381 ggcggataga cggtgtcgag accgtttgcg agcacagcga ccgtgcgccc tccagactcg
     1441 agcgcggtat ggtgggcgac cgtgtcgacg cctcgggcga ggccggagac gacggtgacg
     1501 cggttcgccg ccagtccgcg ggcaaactcg gtcgccgcct gccgcccgta ggcggtcgcg
     1561 cgacgcgtcc ccacgaccgc gacgctccat tcgtcgtcag ggatccagct gcctttcacg
     1621 tacaggagcg gtggcgcgtc ggcgatctca tgcagccgcg aggggtagcg aggatcgtcg
     1681 ggggcgagcg cgtcgacgcc ggccagat
//
LOCUS       DAORPG010000470         1706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9366_length_1706_cov_3.140521, whole genome shotgun sequence.
ACCESSION   DAORPG010000470 DAORPG010000000
VERSION     DAORPG010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9366_length_1706_cov_3.140521"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="PLX85_09320"
     CDS             <1..1205
                     /locus_tag="PLX85_09320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HRC63403.1"
                     /translation="LADPPRTPIELELTGFAAGGRAVGRAPDGRVAFVEFAIPGERVI
                     AEITREESSFVEASTVMVLEASPARVEPRCEYFGRCGGCQLQHINYREQLALKTGLVR
                     EQLRRIGHFEDPPVLDAIGMDDPWGYRNHIRFTVRRDGQVGFMQRGTHRFLRIDYCDI
                     AHARVNEVLRQVQERTMQTAQLSVRVGENTGDVLVQPKLRWRPTRGASVPSGQPHYTE
                     RLRGATYRVSSPAFFQVNTRQAEVLAGLVLRHVTEAHPRVAVDAYAGVGTFAALLADH
                     VEQVVTIEWSAAAGDDGEVNLRPYSNVTRVVGSVEDVLPGLDPAPDVVVVDPPRIGLQ
                     RSVVDTILASPVRRLVYVSCDPSTLARDLRLLVDGGFALREVQPLDMFPHTQHIECVS
                     VLDRLDEA"
     gene            1206..>1706
                     /locus_tag="PLX85_09325"
     CDS             1206..>1706
                     /locus_tag="PLX85_09325"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRC63404.1"
                     /translation="MRLILASGSARRPELLASLGLQFEIIPAGVDESSEERDPERLAR
                     ELALRKANAVATLEPDAVVIGADTLVTLDGRFFGKPADAAEATDTLRALRGHTHRVVS
                     GVAVVSAADHAVEAVVTHVTMRLYADEEIERYVATGSPLDKSGAYAIQDPVFAPVSGF
                     EGCMCSV"
BASE COUNT          252 a          626 c          549 g          279 t
ORIGIN      
        1 gtctcgcgga tcccccccgc actcccatcg agctcgagct caccggcttc gccgcgggcg
       61 gtcgcgcggt cggccgcgcc cctgacggtc gcgtcgcctt cgtcgagttc gccatccccg
      121 gagaacgggt gatcgccgag atcacccgcg aggagtcctc gttcgtcgag gcaagcaccg
      181 tgatggttct cgaggcctcc ccggcccgtg tcgagccgcg ttgcgagtac tttggccgct
      241 gcgggggctg ccagctgcag cacatcaact accgcgagca gctggcgttg aagaccggcc
      301 tcgtccgcga gcagctccgg cgcatcggcc acttcgagga cccgccagtc ctcgacgcca
      361 tcggcatgga cgacccgtgg ggctaccgta accacatccg cttcaccgtc cgccgcgatg
      421 gccaggtcgg cttcatgcag cgcggcaccc atcgcttcct gcgcatcgac tactgcgaca
      481 tcgcccacgc ccgcgtaaac gaggtgctcc ggcaggtcca ggagcgcacg atgcagaccg
      541 cgcagctgag cgtgcgcgtc ggagagaaca ccggcgacgt gctcgtgcag ccaaagctgc
      601 gctggaggcc aacccgcggg gcatccgtgc ccagcggcca gccgcactac accgagcgcc
      661 tccgaggtgc gacctaccgc gtttcctcgc cggcgttctt tcaggtgaac acccggcagg
      721 ccgaggtgct cgcgggcctc gtcctgcgcc acgtcaccga ggcgcaccca cgcgtcgccg
      781 tcgacgccta cgcgggcgtc ggcacgttcg cggcgctgct tgcggaccac gtcgaacagg
      841 tcgtcacgat cgagtggtcg gccgcggccg gagacgacgg cgaggtgaac ctccgaccct
      901 actcgaacgt cacgcgggtc gtcgggagcg tggaggacgt gctgcccggt ctcgaccccg
      961 cgcctgatgt cgtcgtcgtc gacccgccgc gcattggcct tcagcgttcc gtcgtcgaca
     1021 ccatcctggc ctccccggtt cgccggctgg tctacgtgtc ctgcgatccc tcgacgctcg
     1081 cgcgcgacct gcgactcctc gtggacggcg gctttgccct ccgcgaggtg cagccgctcg
     1141 acatgttccc gcatacgcaa cacatcgagt gcgtatccgt cctcgaccgg ttggacgagg
     1201 cgtaaatgag gctgatcctc gcctcgggct ccgcgcgccg tcccgaactc cttgcgtcgc
     1261 tcggtcttca gttcgagatc atccccgccg gcgtcgacga atcctccgag gagcgcgacc
     1321 ccgagcgact ggcacgagaa cttgcgctgc gcaaagcaaa tgcggtcgcc acgctcgaac
     1381 ctgatgccgt cgtgatcggc gccgacacac tcgtcaccct cgacggacgc ttcttcggca
     1441 agccagcgga tgcggcggag gccacagaca cgctgcgcgc cctgcgcggg cacacccacc
     1501 gcgtcgtcag tggcgttgcg gtcgtgtcgg cggcagacca tgcagtcgag gcggtcgtca
     1561 ctcacgtgac gatgcgcctg tatgccgacg aggagatcga gcggtacgtg gccacgggct
     1621 cgcccctcga taagtcggga gcctacgcca tccaggatcc ggtctttgcc ccggtctcgg
     1681 gcttcgaagg atgtatgtgc tcggtg
//
LOCUS       DAORPG010000471         1706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9369_length_1706_cov_2.574803, whole genome shotgun sequence.
ACCESSION   DAORPG010000471 DAORPG010000000
VERSION     DAORPG010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9369_length_1706_cov_2.574803"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1706
                     /locus_tag="PLX85_09330"
     CDS             <1..>1706
                     /locus_tag="PLX85_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRC63405.1"
                     /translation="RELTSTRAQWGRTYEVRAGDEALAGEAVAIHSPSHPDEGPVGVV
                     PFVDAETARRVASAVREGSTPWATTPVGERARILRRAADLLEERAHEFAAWIVHEGGR
                     DRAGAWAEVVEAVDYLRYYAVQAEQLFRDFEGRVAPRGVVAVIPPWNFSLAIPCGMTV
                     AALATGNGVVLKPAGQTPLVALRLTALLHEAGVPRGALVCLPGHGSEAGQALVESPDV
                     AMVAFTGSRGVGVRMHDDVARVIPRDGVVKALVAEMGGKNAALVFADADMDEAVDSIV
                     HSSFGHANQKCSAVSRVLVERSVLDRLSARLVEAARSLHIGPADAAATEVNPIIDRRA
                     WDRLQSAAAEARREGEVLLDLFDATALQAGPLIVQMDAAQALSSSTATEELFGPILVV
                     IPFDDEEEVYDIANGTAYGLTAGVYSRSPSTVERATSALRAGNIYVNRGTTGARPGVE
                     PFGGMAMSGTGPKAGGPDSLWAFVRRTDVPEDERGTLEGVDGIATDTAAPRLAPRWEA
                     SLEDRIAVLERCAARVGGGDTDEALQILDVVQAARRELGRPAPTVQAAGQHTELRYDW
                     PRG"
BASE COUNT          247 a          529 c          691 g          239 t
ORIGIN      
        1 cgtgaactga cctcgacgcg ggcccaatgg ggacggacgt acgaggttcg agccggcgac
       61 gaggcgctcg ccggcgaggc ggtagcgatc cattcgccat cgcaccccga cgaggggccg
      121 gtcggcgtcg tgccgttcgt cgacgccgag acggcgcgac gcgtggcctc ggcggtgcgc
      181 gagggaagca cgccgtgggc gacgacgccg gtgggcgaac gagcccggat actgcggcgg
      241 gcggcggatc tcctcgaaga gcgtgcgcac gagtttgctg cctggatcgt gcacgaaggg
      301 ggacgcgacc gggcgggggc gtgggcggag gtcgtcgaag cggtcgatta cctaaggtac
      361 tacgccgtgc aggccgagca actgttccgg gacttcgagg ggcgcgtcgc tcctcgcggg
      421 gtggtggcgg tcatcccgcc gtggaacttc tcgctggcga tcccgtgcgg gatgacggtg
      481 gcggcgctgg cgacggggaa cggcgtcgtg ctgaagccgg ctggtcagac gccgctggtc
      541 gcactgcggc tgaccgcgct gttgcacgag gccggggtgc ctcggggggc gctggtgtgc
      601 ctgcccgggc acggctccga agcggggcag gcgctcgtgg agagcccgga cgtggcgatg
      661 gtggcgttta ccggctcccg cggcgtgggc gtgcggatgc atgacgacgt ggcgcgggtc
      721 atacctcgag acggcgtcgt gaaggcgctc gtggcagaga tgggcggcaa gaacgccgcc
      781 ctcgtgtttg cggatgcgga catggacgag gccgtcgaca gcatcgtgca ctcctcgttc
      841 gggcatgcga accagaagtg ctcggccgtg tcgcgtgtcc tcgtcgagcg gtccgtcctg
      901 gatcgacttt cggcccgact cgtcgaggcc gcacgcagcc tacacattgg cccggcggac
      961 gcggcggcga cggaggtgaa cccgatcatc gaccggcgcg cgtgggatcg actccagagc
     1021 gcggcggccg aggcgcggcg cgaaggcgaa gtcctcctcg acctgttcga cgcgacggcg
     1081 ctgcaggcgg ggccgttgat cgtgcagatg gacgccgcgc aggcgctgtc ctcgtccacg
     1141 gcgaccgagg agctgttcgg gccgatcctc gtcgtgatcc cgttcgatga cgaggaggag
     1201 gtctatgaca tcgcgaacgg gacggcgtac gggctgacag ccggcgtgta ctcgcgcagc
     1261 ccctcgacgg tggagcgagc tacctcggcg ctacgggcgg ggaacatcta cgtgaaccgc
     1321 gggacaacgg gcgcgcggcc gggggtggag ccgttcggcg ggatggcgat gtcggggacg
     1381 gggccgaagg cgggcgggcc cgactccctg tgggcgttcg tacggcgcac agacgtaccc
     1441 gaggacgaac gtggcactct cgaaggcgtc gatggcatcg cgactgacac ggcggccccc
     1501 aggctggctc cgaggtggga ggcaagcctc gaagatcgca tcgcggtcct cgagcggtgc
     1561 gccgcgcgag tcggcggagg cgacaccgac gaggcgctgc agatcctcga tgtggtgcag
     1621 gcggcgaggc gcgagctggg tcgtcccgcg ccgacggtgc aggccgcggg gcagcacacc
     1681 gagctgcgct acgactggcc tcgcgg
//
LOCUS       DAORPG010000472         1703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9394_length_1703_cov_3.283981, whole genome shotgun sequence.
ACCESSION   DAORPG010000472 DAORPG010000000
VERSION     DAORPG010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9394_length_1703_cov_3.283981"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="PLX85_09335"
     CDS             complement(<1..693)
                     /locus_tag="PLX85_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HRC63406.1"
                     /translation="MEVRAIAGNLAEQQVETLVVEVTEGASRLSGPTAQVDAALGGAI
                     TAMIRDGLISGRAGEVTSIAALGRVPARRVVVHGAGKAEARTIDGFRTRAGNLARALR
                     GLDAKSVAVGLQEADSLDAETVGRALAEGFVLGLYRFDRHHTRAEDRPRGAVDAITLV
                     EAAPRRLPALQRGVEAGTILAEATNLTRDLANEPANLMTPTIVAERAQEVAAATGLEC
                     TIIERAEAERLGM"
     gene            846..1685
                     /locus_tag="PLX85_09340"
     CDS             846..1685
                     /locus_tag="PLX85_09340"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRC63407.1"
                     /translation="MPLHRRLARSMLLATSPQRQGVGWWDVSEVALVALGFLLYFLVR
                     GAPADRTEDALQHARSIVDLERSLGMFIEPHVQEWVLSAGILWRLMNFVYFWFDFPLI
                     VGVGLFMFFRSRTYYTLLRDSLLLSGGLALLIYYTFPVAPPRYLTDLGFVDTLEVYSN
                     LSYQAQSMQPFVNPYAAVPSLHVGWSALLAYAVFRATANVFARAAVLGVFVLQCIAVM
                     GTGNHFAFDGFVGLLVCALGLAMAMWLQRAGYPAVRGRLAAWADIAPGGEARRRRHEA
                     RAG"
BASE COUNT          218 a          569 c          616 g          300 t
ORIGIN      
        1 catacccagg cgttcggcct cggcgcgctc gatgatggtg cattcgaggc cggtcgcggc
       61 cgcgacctcc tgcgcgcgct cggcgacgat cgtgggggtc atcaggttgg ccggctcatt
      121 ggcgaggtcg cgggtcaggt tcgtggcctc cgcgaggatc gtccccgcct cgacgccacg
      181 ctggagtgcc gggaggcggc gtggggccgc ctcgacgagg gtaatggcgt ccacggcccc
      241 gcgcgggcgg tcctcggcgc gtgtgtgatg gcggtcgaag cggtagaggc cgagaacgaa
      301 gccctcggcc agcgcgcggc caactgtttc ggcatcgagg gagtcggcct cctggagccc
      361 gacggcgacg gacttcgcgt cgagcccgcg cagggcgcgc gcgaggttgc cggcccgtgt
      421 acggaacccg tcgatggtgc gtgcctcggc cttgcccgcg ccatggacca ccacgcgcct
      481 ggcgggcacg cgcccgagag cggcgatcga ggtgacctcc ccggcgcggc cggagatgag
      541 gccgtcgcgg atcatggcgg tgatcgcgcc gccgagggcg gcgtcgacct gtgccgtggg
      601 gccgctgaga cgactggcgc cctcggtcac ttccacgacg agcgtctcga cctgctgctc
      661 ggcgaggttg ccggcaatcg cgcgcacttc catctcggac tcccgttctc cgcctcggtt
      721 cgctggcggg aggatagtga ggagccgcac agggccgcaa acccgcgtac ggccgcggac
      781 acccggccga gggcgaattg ctacaatggc acttcacgaa tcgaactgaa gtactggagc
      841 cggacgtgcc actgcaccgt cgcctggccc gctcgatgct gctggcgacc agcccgcagc
      901 gacagggcgt gggctggtgg gacgtgtccg aggtggcgct ggtcgcgctc gggttcctgc
      961 tctacttcct cgtgcgaggt gcgccggccg accggaccga ggatgcactc cagcacgctc
     1021 gctcgatcgt cgacctcgag cgctcgctcg gcatgttcat cgagccgcac gtccaggagt
     1081 gggtgctgag cgccgggatc ctctggcggc tcatgaattt cgtctacttc tggtttgact
     1141 tcccgctcat cgtcggcgtc ggactgttca tgttcttccg cagccgcacg tactacacac
     1201 tgttgcgcga ctcgctgctg ctgtcgggcg gactcgcgct gctgatctac tacacgtttc
     1261 cggtcgcgcc gccccgttat ctgacggatc tcgggttcgt cgacaccctc gaggtgtatt
     1321 cgaatctgtc atatcaggcg cagtcgatgc agccgttcgt gaacccgtac gcggccgtgc
     1381 cttcgttgca cgtgggatgg tcggcgttgc tcgcatacgc cgtgttccgc gcgacggcga
     1441 acgtgttcgc gcgcgccgcg gtcctcggcg tcttcgtcct gcagtgcata gcggtgatgg
     1501 ggacgggcaa ccacttcgca ttcgatggct tcgtcgggct gcttgtctgc gcgctcggtc
     1561 tggcgatggc catgtggctg cagcgagccg gataccccgc ggtgcgcggt cggctggcgg
     1621 cctgggcgga catcgcaccc ggcggcgagg cgcgccggcg ccggcacgag gcacgcgcgg
     1681 ggtaaccggg gggcagcgag tca
//
LOCUS       DAORPG010000473         1702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9405_length_1702_cov_3.124469, whole genome shotgun sequence.
ACCESSION   DAORPG010000473 DAORPG010000000
VERSION     DAORPG010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9405_length_1702_cov_3.124469"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="PLX85_09345"
     CDS             <1..1335
                     /locus_tag="PLX85_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC63408.1"
                     /translation="DWRLGIAALVVLPLFLPTGLIMGPRTARASMTRQTDLGTVATEL
                     QENLGGQFVVRTFGLEGQSIGSFARVADQLLRSSIRLTFLSSIYGVTTETLSWIVQLL
                     TIGLGGWLVIEGELSLGTMIAFLGLLGLMTTPVQNIAALVQVVQQATGALVRVEEVLA
                     LPVERVDSGRPLRPVVTSLEFEHVSFSYGTGQAVLNDVSFRIPAGSRFAIVGPSGSGK
                     STVVRLLLRVEEPESGQFLIDGIDGRQFSRGSVRQQLAVVPQDDFLFNVPIAENVRLG
                     RPGATDAEVREAIAAAELEQFVEQLPEGVGTVVGERGGQLSGGQRQRVALARALLRKP
                     SVLILDEATSALDAETEAAILGTIGRLDRAVTVVMVTHRLASVRDADWIVVLDGGRVV
                     EEGRHRALLLERGVYRRLWDAQSRGGSGNEEGSPLLAGPTSEARSGEVGAGG"
     gene            1537..1665
                     /locus_tag="PLX85_09350"
     CDS             1537..1665
                     /locus_tag="PLX85_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63409.1"
                     /translation="MFLWQSNKQIQKNVSGFSLLAIQTNIALGGNVAILVMAPPAP"
BASE COUNT          266 a          490 c          632 g          314 t
ORIGIN      
        1 gactggcgac tcgggatcgc ggcgctggtg gtgttgccgc tgttcttgcc gaccgggctg
       61 atcatggggc cgcgtacggc gcgggcgagc atgacccggc agaccgatct cggcaccgtg
      121 gccacggagc tgcaggagaa cctgggcggg caattcgttg tgcggacctt cggcctcgag
      181 gggcagtcga tcgggagctt cgcacgcgtg gcagaccagc tgctgcggtc ctcaatccgg
      241 ttgacgttcc tgtcgtcgat ttacggggtt acgacggaga cgctcagctg gattgtgcag
      301 ctgttgacca tcggcctcgg tggttggctc gtgatcgagg gggagctcag cctggggacg
      361 atgatcgcct ttctcggact gctggggctc atgacgacgc ctgtgcagaa catcgcggcg
      421 ctggtccagg tcgtgcagca ggcgacgggg gcgctcgtgc gcgtcgagga agtgctggcg
      481 ctcccggtcg agcgcgtgga cagcggccgc ccgttgcgac ccgtggttac gagcctggag
      541 ttcgagcacg tttccttcag ttacggcacc gggcaagcgg tgctgaatga tgtctcgttt
      601 cgcattcccg ccggttcgcg tttcgcgatc gtgggaccaa gcgggtctgg gaaatcaacg
      661 gtcgtgcgtc ttctgctgcg agtcgaggag ccggagagcg ggcagttcct catcgacggc
      721 atcgacggcc ggcagttcag ccgcgggtcg gtacgtcagc agctggcggt ggtgccgcag
      781 gatgacttcc tgttcaacgt cccaatcgcg gagaacgtgc gactcgggcg acccggcgcc
      841 accgacgcgg aggtgcggga agccatcgcg gcggccgaac tcgagcagtt cgtggaacag
      901 cttccggaag gcgttgggac ggttgtcggg gagcggggag ggcaattgtc tggcgggcag
      961 cggcagcgcg tggcgctcgc gcgggcgttg ttgcgcaagc cctcggtatt gatcctcgac
     1021 gaggcgacgt cagcgctgga cgcggagacc gaagcggcga tcctgggcac tatcgggcgt
     1081 ctcgaccgtg ctgtgaccgt cgtgatggtg acgcatcggc tggcgagcgt gcgtgatgcg
     1141 gactggattg tcgtgctcga cggcgggcgc gtcgtcgagg aagggcgcca tcgggcgctg
     1201 ctcctcgaac ggggcgtcta tcgccggctg tgggatgcgc agagcagggg aggatcgggc
     1261 aatgaggaag gttcccctct cctcgccggt cctacgtccg aagctcggtc gggcgaggta
     1321 ggagcgggag gctgagttgc gcgcggagcc ggggcagcag gggaggtcgc tgcgcgatta
     1381 tgtctgaaag ctcctcgacg cgaccgagct tcgtcgccag ctcatccgcg atctgataac
     1441 gcaatcactc ggctttttat tgacatggcc tctgatgcgt tgtgtaatga tggccgcgcc
     1501 tccagaggct tacccagaga gaaaggtacc caagacatgt tcctgtggca gtcgaacaag
     1561 cagatccaga agaacgtgag cggcttcagc ttgctcgcga tccagaccaa catcgccctc
     1621 ggtgggaacg tggcaatcct ggtgatggcc ccgccggctc cgtaggcctt gcagcccggg
     1681 ccggcgcccg ttgaggcgac gc
//
LOCUS       DAORPG010000474         1700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9427_length_1700_cov_3.165350, whole genome shotgun sequence.
ACCESSION   DAORPG010000474 DAORPG010000000
VERSION     DAORPG010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9427_length_1700_cov_3.165350"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="PLX85_09355"
     CDS             <1..885
                     /locus_tag="PLX85_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRC63410.1"
                     /translation="VRRSAMFMDTTVTIEVAHRDEEPAALDAIERAMRWFERVEATCS
                     RFDPESELRGLTAQAGVPWHVSALLIEPLAFALALAEETNGAFDPTVGGRLEAAGFDR
                     NYRTGARAGAGLESTEGSYRDVEIDRAASTVTLHRRLVLDLGAVAKGFAVDLAARELA
                     SFERVAVFAGGDVLVRAPAGEPYEIGIQHPRDPDALLAVLGLSEGAVCTSGDYQRTSA
                     AGHHIVEPGTGQAVGGVVSTTVVAPTAMLADGLSTAAFVLGVPAGLRLVEANGGEGLL
                     VTRDLERVMTSSFGRLAL"
     gene            882..>1700
                     /locus_tag="PLX85_09360"
     CDS             882..>1700
                     /locus_tag="PLX85_09360"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HRC63411.1"
                     /translation="MTRAAQRGLRFFRTPKGLMLIILVALLGVAAPNSAGDGPRLLAA
                     GALAAAALDVMIVYGMWGEWAFPSGALLTGLFIGGLVDPFERVQVVVVIAIAAIFAKH
                     IVRMGGSHVFNPAALGLVASSVVFGSGQSWWAALPDLGAIGVAAVAVSGWWIARRLNK
                     LPMVATFLGAYLVLFTVTAIAGSPDQVSEVFRSPDLQAALFFAFFMLTDPPTSPVRYR
                     DQAIYGALVAAGSYAVFMQFGVVYFLLAGLLAGNAVEAGRRTVARRRRRTRSARA"
BASE COUNT          213 a          499 c          714 g          274 t
ORIGIN      
        1 gtgcgacggt cggcgatgtt tatggacacg acggtcacga tcgaggttgc gcatcgcgac
       61 gaggagccgg cggccctcga tgcgatcgag cgggcgatgc ggtggttcga gcgggtcgag
      121 gcgacgtgca gccggttcga cccggagagt gagctgcggg ggctgacggc gcaggcgggc
      181 gtgccctggc acgtcagcgc gttgctgatc gagccgctgg catttgcgct ggcgctcgcg
      241 gaggaaacga acggggcgtt cgaccccacg gtcggggggc gtctcgaggc ggcggggttc
      301 gaccggaact accgcacggg ggcgcgggcg ggcgcggggc tcgaatcgac cgagggcagc
      361 taccgcgacg tggagattga ccgggcggca agcaccgtga ccttgcaccg gcggctcgtg
      421 ctggacctgg gcgcggtggc gaaggggttc gcggtagacc tcgcggcccg ggagctggcg
      481 tcctttgagc gagtggcggt gttcgcgggc ggcgacgtgc tcgtgcgggc gccagccggg
      541 gagccctatg agatcggcat ccagcatccg cgcgacccgg acgcgctgct ggccgtgctg
      601 ggcctgagcg aaggcgcggt ctgcacctcg ggggactatc agcgcacctc ggcggccggg
      661 catcacatcg tggagccggg gaccgggcag gctgtgggcg gagtcgtgag caccacggtc
      721 gtcgcgccga cggcaatgct cgcggacggg ctgtcgacgg ctgcgttcgt gctcggcgtg
      781 cccgcgggat tgcgcctcgt cgaggcgaac ggtggcgagg ggctgctcgt cacgagggat
      841 ctggagcgcg tgatgacgag cagcttcggg agactggcgc tatgacacgg gcggcgcagc
      901 gcgggctgcg gttcttccgc acaccgaagg ggctgatgct gatcatcctc gtcgcgctac
      961 tgggggtggc ggctccgaac agtgccgggg acgggccgcg tctgctggcg gcgggcgcgc
     1021 tggcggcggc ggccctcgac gtgatgatcg tgtacgggat gtggggggag tgggccttcc
     1081 cgagcggcgc gttgctgacc gggctgttca tcgggggcct cgttgacccg ttcgagcggg
     1141 tacaggtcgt ggtggtgatc gcgatcgcgg cgattttcgc gaagcacatc gtgcggatgg
     1201 gcgggtcgca cgtgttcaac ccggccgcgc tcgggctggt ggcgtcctcg gtggtgttcg
     1261 ggagcgggca aagctggtgg gcggcgctgc ccgacctcgg agcgatcggg gtggcggcgg
     1321 tcgcggtgag cggatggtgg atcgcgcggc ggctgaacaa gctgccgatg gtggcgacgt
     1381 tcctcggcgc gtatctcgtg ctgttcacgg tgacggcgat cgcgggaagc ccggaccagg
     1441 tgtccgaggt attccgctca ccagacctgc aggcggctct gttcttcgcg ttcttcatgc
     1501 tgacggaccc gccgacgtcg ccagtgcggt atcgcgacca ggcgatctat ggcgcgctgg
     1561 tggcggcggg cagctacgcg gtgttcatgc agttcggcgt ggtgtacttc ctgctggccg
     1621 gactgctggc cgggaacgcc gtcgaggcag ggcgacggac ggtggcgcgc cgacgccggc
     1681 ggacgcgcag tgcgcgggcg
//
LOCUS       DAORPG010000475         1698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9443_length_1698_cov_3.404139, whole genome shotgun sequence.
ACCESSION   DAORPG010000475 DAORPG010000000
VERSION     DAORPG010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9443_length_1698_cov_3.404139"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..187)
                     /locus_tag="PLX85_09365"
                     /pseudo
     CDS             complement(1..187)
                     /locus_tag="PLX85_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(329..1554)
                     /locus_tag="PLX85_09370"
     CDS             complement(join(329..1245,1245..1554))
                     /locus_tag="PLX85_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611735.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRC63412.1"
                     /translation="MQQDERGKRPSSRRYPAEVKERAVRLVLESHRPGESWYSTVRRV
                     APQLDVGVESLRTWTHQARVDARQLPGASSEEQRRIVELERENRELRRANEILKAASA
                     FFRAGARPASTGQVVEFIDAHREAFGVEPICAVLQIAPSTYYAAKSRPPSARSLTDAT
                     LKQRIATLHADNYDVYGAVKVWRALRREGVEVARCTVARLMGELGLEGARRGGPRHPI
                     TTRPDDAQERPADLVERRFTASRPNELWVADFTYVRSFAGWAYVAFIIDVYSRYIVGW
                     QAATTMRTELVLDALAMARWRRRADSIEGVVHHSDAGSQYLSVRYSERLEEAGAQPSV
                     GSRGDSYDNALAESTIGLYKTELINRRGPWPSIDAIEIATLEYIDWFNHRRLHGEIGH
                     LPPAEYEAAYTTSTRP"
     misc_feature    complement(1136..1252)
                     /locus_tag="PLX85_09370"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          251 a          602 c          565 g          280 t
ORIGIN      
        1 cccccaccag cacgacaccc atcacggccc gcacacgaaa aactgcgcag ctccccttcg
       61 cctccaggtg ggggtcggtc gagaacttga acgtgccacg ccgccagggc tgcacgaggt
      121 agctgcgcca ggcacgcgcg acggacgcgt caccgatccc caactcgcgc gccaacagcc
      181 gcgttgaatc gcgccgggtt tggtggagac tcgatggttt ggcactgtgg ggtgccgggt
      241 cgggtcttct ggtcgagtct agtggtgttt cgtcgggctc gcggagggct cctacttcta
      301 gccacgtagc gagcccgacg gcggcccgtc agggccgcgt gctggttgtg taggcggctt
      361 cgtattcggc gggtgggagg tggccgatct cgccgtgcag gcggcggtgg ttgaaccagt
      421 cgatgtattc gagggtggcg atctcgatcg cgtcgatgct gggccagggg ccgcggcggt
      481 tgatcagctc ggtcttgtag aggccgatcg tggattccgc cagggcgttg tcgtaggagt
      541 cgccgcggct gccgacggac ggctgcgcgc cggcttcctc gagtcgctcg ctgtagcgta
      601 cggagaggta ttgcgagccg gcgtcggagt ggtgcacgac accctcgatg ctgtctgctc
      661 gccgtcgcca gcgtgccatc gccagcgcgt cgagcacgag ctcggtgcgc atcgtggtgg
      721 cggcctgcca gcccacgatg tagcggctgt agacatcgat gatgaaggcc acgtaggccc
      781 agccggcgaa gctgcggacg taggtgaagt ccgccaccca cagctcgttc gggcggctcg
      841 ctgtgaagcg ccgctcgacg agatccgcgg gccgctcttg ggcgtcgtcg ggacgcgtgg
      901 tgatgggatg gcgtggcccg ccgcgacgtg ccccctccag gccgagctcg cccatcagcc
      961 gcgcgaccgt gcagcgcgcc acctccacgc cctcgcgccg cagtgcccgc cagaccttga
     1021 cggcgccgta gacgtcgtag ttgtcggcat gcagcgtggc gatgcgctgc ttgagcgtgg
     1081 catccgtcag cgagcgcgcg ctgggcggcc tgctcttggc ggcgtagtac gtcgacgggg
     1141 cgatctgcaa cacagcgcag atcggctcga ccccgaacgc ctcacgatgg gcgtcgatga
     1201 actccactac ctgcccggta gacgcgggtc gagctcccgc gcgaaaaaag ccgacgccgc
     1261 cttgaggatc tcgttcgcgc gacgcaactc ccgattctcc cgctccaact ccacgatccg
     1321 ccgctgctcc tcgctcgacg cgcccggcag ctgtctggcg tccacgcgcg cctgatgcgt
     1381 ccacgtccgc aacgactcca ccccgacatc gagctgcggc gcgacccgtc gcaccgtcga
     1441 gtaccacgac tcacccggcc gatgcgactc caacaccagc cgcaccgcac gctccttcac
     1501 ctccgccgga tagcgccgcg acgacggccg cttcccccgc tcgtcctgct gcatgactcc
     1561 cccttctcct cacaagatcg ggagcctcca caaaacccgg cgcgattcac gtcgaccagt
     1621 gcgtgacgcc gagccgctca ggcggcggca caagcgtcgc ggcgatgatc tccgcgtcgt
     1681 ccaactgctt gggacgcc
//
LOCUS       DAORPG010000476         1698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9444_length_1698_cov_3.376141, whole genome shotgun sequence.
ACCESSION   DAORPG010000476 DAORPG010000000
VERSION     DAORPG010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9444_length_1698_cov_3.376141"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PLX85_09375"
     CDS             <1..482
                     /locus_tag="PLX85_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63413.1"
                     /translation="PGEAFCFGDGADPFVTTACPCGNFGALGNGCRSSFNPAGAHLDA
                     TGATANDDVALLGTGMNPTGIAIFVKADSQLTTGLLYGDGITCLDGNLIRLRQKPLAG
                     GDARFPDSTDTTTLSARGGTPPGSGAFATYLVYYRNASTTFCPPATANVTNGWTILW"
     gene            complement(495..1508)
                     /locus_tag="PLX85_09380"
     CDS             complement(495..1508)
                     /locus_tag="PLX85_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63414.1"
                     /translation="MPFQPPRCPFDDCSASVPGARFPWRRRGTFVRACDGRVVQRFLC
                     LTCLRSFSTQSFRFDYRLRNPFLHKPVFGHLVSKVTHRQAARLLGVTRKTVELRLRRL
                     GEHCRLFHAARLAGKHIGGVFQLDEAETFETDRRLKPVTMPVLIERKTRFIVHTESAP
                     LPARGNLSPALEARKREYEALEGQRKSGSRAAVKKCFEALAAHLDPAAPLVVQTDRKA
                     TYPGILRDVFTARAVVHETTSSRTKRDGKNLICAINHTLAMLRDGLSRMVRRTWAHAK
                     WRVRLAQHMWVYVVYRNYVRYRSNKEKRATPAMRLGIEVERWTLRDLLRWSARFPELL
                     RVQ"
BASE COUNT          266 a          551 c          619 g          262 t
ORIGIN      
        1 cgcccggcga agcgttctgt ttcggcgacg gcgcggaccc gttcgtgacg accgcatgcc
       61 cgtgcgggaa cttcggcgcg ctcggcaacg gctgccgctc gtcgttcaat cccgccggag
      121 ctcacctcga cgccacgggc gcgaccgcga acgacgatgt cgcgctgctc ggcacgggca
      181 tgaacccgac gggcatcgcc atcttcgtga aggcggattc acagctcacc acagggctcc
      241 tgtacggcga cggcatcacg tgcctcgacg gcaacctgat ccggctgcgc cagaaacccc
      301 tcgcgggcgg cgacgcgcgt ttccccgaca gcacggacac gacgacgttg tcggcgcggg
      361 gtggcacgcc gccggggtcg ggggcgtttg cgacgtatct cgtctactac aggaatgcgt
      421 cgacgacgtt ttgtccgccg gcgacggcca atgtcacgaa cggctggacg atactctggt
      481 gagactggcc ccgttcactg gactcgcaag agttcgggaa acctggcgga ccagcgcagg
      541 agatcgcgca gagtccagcg ctcgacctcg atgccgaggc gcatcgcggg tgtagcgcgc
      601 ttctccttgt tcgagcggta gcggacgtag ttgcggtaca cgacgtagac ccacatgtgc
      661 tgcgcgagcc gcacgcgcca cttcgcgtgc gcccaggtgc ggcgcaccat gcgcgacagg
      721 ccgtcgcgca gcatcgcgag cgtgtggttg atggcgcaga tcaggttctt gccgtcgcgc
      781 ttggtccgcg acgaggtcgt ctcgtgcacc acggcgcggg cggtgaacac gtcgcgcagg
      841 atgccggggt acgtcgcctt gcgatcggtt tggacgacga gcggcgctgc gggatcgagg
      901 tgtgcggcga gcgcttcgaa acacttcttc accgcggcgc gcgagccgct cttgcgttgg
      961 ccctcgagcg cttcgtactc gcgcttgcgc gcctcgagcg ccggcgagag gtttccgcgc
     1021 gcgggaaggg gcgccgattc ggtgtgcacg atgaagcgcg tcttgcgctc gatcagcacc
     1081 ggcatcgtca cgggcttgag gcggcggtcc gtctcgaagg tctcggcttc gtcgagctgg
     1141 aagactcccc cgatgtgctt gccggcgagg cgcgccgcgt ggaagaggcg gcagtgttcg
     1201 ccgaggcggc gcaggcgcaa ttcgaccgtc ttgcgcgtca cgccgagcag gcgcgcggcc
     1261 tggcggtgcg tgaccttcga gacgaggtgg ccgaagacgg gcttgtggag gaaggggttt
     1321 cgcaggcggt agtcgaagcg gaaggactgc gtcgagaagc tgcgcaggca ggtgagacag
     1381 aggaaacgct gcacgacgcg gccgtcacag gcgcgcacga aggtgccgcg gcgccgccag
     1441 gggaaacggg cgccggggac ggatgcggag cagtcgtcga acggacaacg cggcggctgg
     1501 aacggcatga gggattcctc cgcggaccca tgaccttggg cccgccgtcc gcacgacgct
     1561 cggtcagcgg aacggtcgcc gtttttctgc gagaaatcgg tctggccgag tgaaatcgac
     1621 gccagtcagc ggggccagtt tctctcaagg ggcggagggg cgggcgacga accccccctc
     1681 cccccccccc cgccgccc
//
LOCUS       DAORPG010000477         1695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9470_length_1695_cov_3.402439, whole genome shotgun sequence.
ACCESSION   DAORPG010000477 DAORPG010000000
VERSION     DAORPG010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9470_length_1695_cov_3.402439"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PLX85_09385"
     CDS             <1..672
                     /locus_tag="PLX85_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRC63415.1"
                     /translation="KEPEEGDSLTQAVLLGDAAFTHAASLAHPGMTERGLAWEVQRFA
                     MEHGAADLSFPTIIATGPWGALPHAYPRDVPLEPDAGVVMDLGVIVDGYCSDLTRTIF
                     LGETDARFREIYDIVLTAQEMAEERIEAGMTGEQAHQIAATVIAQAGYADYFGHGLGH
                     GVGLDIHEAPRLGPRSSDILVDGQVVTVEPGIYLPGWGGIRIEDQCVMENGRLRRLST
                     AAKLN"
     gene            732..1295
                     /gene="efp"
                     /locus_tag="PLX85_09390"
     CDS             732..1295
                     /gene="efp"
                     /locus_tag="PLX85_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014073324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRC63416.1"
                     /translation="MISTGEIKRGVSVEVDGNLYQVVEVQHVKTKKSAVYRVKMRDLR
                     GGHIIERTFNAGEKLAAARVERRDMQFLYSDASGLTFMNTQTFDQIAVPASIAGNALS
                     YLKEGDVVQVLAYGEEALGIELPPAVQLKIVRSDPGVKGDTATGATKPATLETGLVVN
                     VPLFINEGDTIKVGTSEGNYIERVSNA"
     gene            1320..>1695
                     /locus_tag="PLX85_09395"
     CDS             1320..>1695
                     /locus_tag="PLX85_09395"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF025118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRC63417.1"
                     /translation="MYPVHQVTRYLRELLETNRHLTEVWVSGEVSNLSLASSGHIYFT
                     LKDGRGALRCAFFRNRNVGQRDRLHEGASLIVYGSLSLYEQRGDLSFVVDFVQPEGTG
                     ALAAEFERRRARFEEEGLFASER"
BASE COUNT          302 a          554 c          567 g          272 t
ORIGIN      
        1 aaagagcccg aggaagggga ctcactcacc caggccgtcc ttctcggcga tgcggcgttt
       61 acgcacgcgg cgagcctcgc gcaccccggc atgaccgaac gtggactcgc atgggaagtg
      121 cagcggtttg ccatggagca cggcgccgcc gacctctcgt tcccgacgat catcgccacc
      181 ggcccgtggg gcgcccttcc gcacgcctac ccgcgcgatg tgccgctcga gccggatgcc
      241 ggggtcgtga tggacctcgg cgtgatcgtg gacgggtatt gctccgacct cacccgcacg
      301 atcttcctcg gtgagactga cgcccgcttt cgcgagatct acgacatcgt gctgacagcg
      361 caggaaatgg cggaagagcg catcgaggca ggcatgaccg gcgagcaggc gcaccagatc
      421 gcggcgaccg tgatcgcgca ggcgggttac gctgactact tcggccatgg gctgggccac
      481 ggcgtcggac tcgacatcca cgaggcgccg cgcctggggc cgcgctcctc ggacatcctc
      541 gtcgatgggc aggtcgtcac tgtggagccc ggtatctatc tccccggatg gggtggcatc
      601 cgcatcgaag atcagtgcgt catggagaac gggcgcttgc gcaggctctc cacggctgcg
      661 aagttgaatt agagtccgaa ggacgcggca agccctgccc gtcacgcacg agtcacagta
      721 gggagtcacc ggtgatcagc actggcgaaa tcaagcgcgg cgtttccgtc gaggtcgatg
      781 gcaacctcta ccaggtcgta gaggtgcagc acgtcaagac gaagaagtcc gccgtatacc
      841 gggtgaagat gcgcgacctg cgcggtggcc acatcatcga gcggacgttc aacgcgggcg
      901 agaagctggc ggcggcccgc gtcgagcggc gcgatatgca gttcctctac tcggacgcga
      961 gcggcctcac gttcatgaac acccagacct tcgaccagat cgcggtaccc gcgagcatcg
     1021 ccggcaacgc gctctcctac ctcaaggagg gcgacgtcgt tcaggtcctc gcctacggcg
     1081 aggaggcact cggcatcgag ctgcccccgg cggtgcagct caagatcgtc cgttccgacc
     1141 cgggagtgaa gggcgacacc gcgaccggcg caacaaagcc agcgaccctc gaaacgggtc
     1201 tcgtcgtgaa cgtgccgctg ttcatcaatg agggcgacac gatcaaggtc ggcacgagcg
     1261 aaggcaacta catcgagcgc gtctcgaacg cgtagccggc ccgcgccatg gctcccgagg
     1321 tgtatccggt ccaccaggtc acgcggtatc tgcgcgagct gctggagacc aaccgtcacc
     1381 tgaccgaggt ctgggtgtcg ggcgaggtgt ccaacctctc cctcgcctcc tcagggcaca
     1441 tctacttcac gctgaaagat ggccgaggcg ccctgcgctg cgcgttcttc cgtaatcgta
     1501 acgtggggca acgcgaccgg ctgcacgagg gcgcctcgct gatcgtgtac gggtcgctct
     1561 cgctgtacga gcagcgggga gacctgtcgt ttgtggtcga cttcgtccag ccggagggca
     1621 ccggcgcgct ggccgcggag ttcgagcgtc gccgggcccg cttcgaggaa gaggggctgt
     1681 ttgcgtccga gcgca
//
LOCUS       DAORPG010000478         1694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9482_length_1694_cov_3.252593, whole genome shotgun sequence.
ACCESSION   DAORPG010000478 DAORPG010000000
VERSION     DAORPG010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9482_length_1694_cov_3.252593"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLX85_09400"
     CDS             complement(<1..261)
                     /locus_tag="PLX85_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63418.1"
                     /translation="MTTVGVLVTEAVAVEVAVFVGVEVGVVCVVGVLVDVEVGVLVAV
                     DVGVVVAVPVGVGVACAVGVLVGVDVGVDVGVEVGVDVGVEVG"
     gene            complement(583..>1694)
                     /locus_tag="PLX85_09405"
     CDS             complement(583..>1694)
                     /locus_tag="PLX85_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRC63419.1"
                     /translation="DLNAGVPDRASLPWRELAEAAARALEADPGGALTYGGTLGYEPL
                     RAWIAERNAAETGLELNSSWVTLTSGSAHAIDNIAATFTAPGDVVVTGAPTYPGAIRT
                     FRARGAEIVTVPQDGDGLEMGPLASALEAQRAAGRPVKLIYVVANYDNPTGATLPEER
                     RIELARLAAEYRALIVEDDAYTGIDLDGPPPTSLFQIMGGEGILRVGTFSKTIATGLR
                     SAWILATPRVIQALTYMRFDNGASPFVQRTIFEYLKTGAYDPHVARLQGIYRERRDAA
                     VSALRERCDSFVTFEVPRGGFFLWLRLREGLEARALARAATEVGVAITPGQMYYAYEG
                     GERNVRLVFSALPPEQLHDAITQLGRACAAARVTP"
BASE COUNT          257 a          682 c          532 g          223 t
ORIGIN      
        1 cccgacctca acaccgacgt ccaccccgac ctcaacaccg acgtccaccc cgacgtcgac
       61 gccgaccagc acgcccacgg cacaggccac cccgacgccg acgggcacgg cgaccaccac
      121 cccgacgtca acggccacca gcacgcccac ctcgacgtcg accagcacgc cgacgacgca
      181 gacgacgcca acctcgaccc caacgaagac ggccacctcg accgccaccg cctcggtgac
      241 gagcacgccc accgtggtca tctcggtcgc tggcgcgacc ccgcctgctg gcgcccagcc
      301 ttcgagcggc gccgtggggc aggtcgcccc accggccatc ggtgttcagg gcgccgcgcc
      361 gattgcgccg cgtccgccca acacgggtaa cgccggcctc ggtaccagga ccacgagccc
      421 cgcgacggcc ttcgcctggc tcggcctcgc cggcctgctt accgcggctg ctcgctactc
      481 ggtgctgacg agaagccgcc gctcgtaacc gcggctcgct gcagcgcaaa gcaaagcggg
      541 ggaggctgag cctcccccgc tttggtgatc gttgccgcaa cgctacgggg tcacgcgcgc
      601 cgcggcgcag gccctcccaa gctgggtgat cgcgtcgtgc agctgctccg gcggcagcgc
      661 cgagaacacc aggcgcacat tgcgctcgcc gccttcatac gcgtagtaca tctgcccggg
      721 cgtgatcgcc accccgacct cggtcgccgc gcgggccagc gcccgtgcct ccagcccctc
      781 ccgcaaccgc agccacagga aaaacccgcc tcgaggcacc tcgaacgtca cgaacgagtc
      841 gcatcgctct cggagcgcgc tcaccgccgc gtcgcgccgc tcacggtaga tcccctgcag
      901 ccgcgcgacg tgcgggtcgt acgcccccgt cttcaggtac tcgaagatcg tgcgctggac
      961 gaacggcgag gcgccgttgt cgaaccgcat atacgtgagc gcctggatga ctcgaggcgt
     1021 cgcgaggatc cacgccgacc gcaggccggt cgcgatcgtc ttcgagaacg tccccacgcg
     1081 caggatgccc tcgcccccca tgatctgaaa caacgaggtg ggcggcgggc catcgaggtc
     1141 gatcccggtg tacgcgtcgt cctcgacgat caatgcgcgg tattccgcgg cgagccgcgc
     1201 cagctcgatg cgccgctcct cgggcaacgt cgcccccgtc gggttgtcat agttcgcgac
     1261 gacgtagatc agctttaccg gacggcctgc cgcccgctgc gcctcgagcg cgctggccag
     1321 cggccccatt tcgagtccgt cgccgtcctg aggcaccgtc acgatctcgg cgccccgagc
     1381 gcggaacgtc cggatcgccc cggggtacgt cggggcgccc gtcaccacta cgtcgcccgg
     1441 cgccgtgaac gtggccgcga tgttgtcgat cgcgtgcgcg ctgcccgagg tgagcgtcac
     1501 ccagctcgag ttcagctcaa gccccgtctc ggcggcgtta cgctccgcga tccaggcgcg
     1561 cagcggttcg taacccagcg tccccccgta cgtcagcgcc ccaccggggt ccgcctcgag
     1621 ggcgcgggcc gcggcctcgg ccagctcgcg ccagggaagg ctggcgcggt ccggcacgcc
     1681 ggcgttcagg tcat
//
LOCUS       DAORPG010000479         1687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9545_length_1687_cov_3.409314, whole genome shotgun sequence.
ACCESSION   DAORPG010000479 DAORPG010000000
VERSION     DAORPG010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9545_length_1687_cov_3.409314"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="PLX85_09410"
     CDS             <1..166
                     /locus_tag="PLX85_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC63420.1"
                     /translation="PAAGTALDEQLEGIERNAILKALEQTRYNKTAAARLLGMTFRAL
                     RYKIKKLGIE"
     gene            378..866
                     /locus_tag="PLX85_09415"
     CDS             378..866
                     /locus_tag="PLX85_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HRC63421.1"
                     /translation="MKTLQKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRAQVTEGL
                     NLAADLKAAVAENFAGTGAWPANNAAVGITAVKSGKYVSGVVINTGTIQITFSNVAPN
                     QANANLNGLVLGLRPTVSANGDVIWNCGYKAVVGADPASGAAAANGTTVTAKYLPQTC
                     RA"
     gene            1078..>1687
                     /locus_tag="PLX85_09420"
     CDS             1078..>1687
                     /locus_tag="PLX85_09420"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HRC63422.1"
                     /translation="MSENATLVMSGARGGLGGLPQRLVQDGVVDESGMHAALQAATEK
                     RTSFVTQLIAAGTASARDIAQAASSEYGVPLLDLNALAVDIDTVKTVSDKLLQKHRVL
                     PLFKRGKRLFLGVADPTNLHAIDEIKFQTGLAVEAIVVEDDKLQKVLDKAIEQVDTSM
                     PNLGEEEGVDLESLEVSGGDEDLEGAVTRDDVEDAPIVRFVNK"
BASE COUNT          322 a          576 c          537 g          252 t
ORIGIN      
        1 ccccgcggcc ggcacggcgc tcgacgagca gctcgagggc atcgagcgca acgccatcct
       61 caaggcgctc gagcagacgc gctacaacaa gaccgccgcg gcccggctcc tgggcatgac
      121 cttccgggcg ctgcgctaca agatcaagaa gctcggcatc gagtgacgct gcgcgtcact
      181 tcctgacgcg gcgggcctga atgtgtcggg aaaagcgggc acgcccgatg tgccgcatat
      241 gtgaaccacg aatatttccc gttggccaac aatgagatat gccgcacggt gccggttggc
      301 acgcgcattg cactatccag cccagcggcg gatgccgtgg acttgaacga ccccaacctc
      361 gttacaacgg agctgacatg aagaccctgc agaaaggctt tacgctgatc gaactgatga
      421 tcgtcgtggc gatcatcggc atcctggccg ccatcgcgat cccggcgtac caggactaca
      481 ccatccgcgc ccaggtgacg gaaggcctga accttgccgc cgacctcaag gccgcggttg
      541 ccgagaactt cgccggcacg ggcgcctggc ccgccaacaa cgctgccgtg ggcatcacgg
      601 ccgtgaagtc gggcaagtac gtctcgggcg tggtcatcaa caccggcacg atccagatca
      661 ccttcagcaa cgtggcgccc aaccaggcga acgcgaacct gaacggcctg gtgctcggcc
      721 tgcgtccgac ggtcagcgcc aacggcgacg tgatctggaa ctgcggctac aaggccgtgg
      781 ttggcgccga cccggcttcg ggtgccgccg ccgcgaacgg cacgacggtc acggcgaagt
      841 acctgccgca gacctgccgc gcctaaagcc tgcggccggg cggaagggtt ttccccttcc
      901 aagaccggtt ccagactgaa tgaagcccct ccctcgtgga ggggcttctt ttttgggagg
      961 ccggtcactt aagcccccct gccctctatg caaacatccg gaaggatttg acattgatcc
     1021 tgcggtaacc ttggccttta aggggtccgt caggagcccg gagtcgcgcg taagtccatg
     1081 agcgagaacg caaccctggt gatgagcggg gcccgtgggg gcctcggcgg gctgccccag
     1141 cggctcgtgc aggatggcgt tgtcgatgaa tcgggcatgc acgccgcgct ccaggccgcc
     1201 accgagaagc gcaccagctt cgtcacccag ctcatcgccg ccggcacggc gagcgcccgc
     1261 gacatcgcgc aggccgcctc gagcgaatac ggcgtgccgc tgctcgacct gaacgccctc
     1321 gcggtcgaca tcgacaccgt caagacggtc tccgacaagc tgctgcagaa gcaccgcgtc
     1381 ctgccgctgt tcaagcgcgg caagcgcctg ttcctcggcg tcgccgaccc gaccaacctg
     1441 cacgccatcg acgagatcaa gttccagacc gggctcgcgg tcgaggcgat cgtcgtcgag
     1501 gacgacaagc tgcagaaggt gctcgacaag gcgatcgagc aggtcgacac ctccatgccg
     1561 aacctcggcg aggaggaagg cgtcgacctc gagtcgctcg aggtcagcgg cggcgacgag
     1621 gacctcgagg gcgcggtcac ccgcgacgac gtcgaggacg cgccgatcgt ccgcttcgtc
     1681 aacaagg
//
LOCUS       DAORPG010000480         1687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9547_length_1687_cov_3.156250, whole genome shotgun sequence.
ACCESSION   DAORPG010000480 DAORPG010000000
VERSION     DAORPG010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9547_length_1687_cov_3.156250"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="PLX85_09425"
     CDS             <1..739
                     /locus_tag="PLX85_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRC63423.1"
                     /translation="RAFYTWLDREALLPSAQPGDSILRLRTPAAPRRLPQFLTAEEAT
                     SLVEAPPADTARGLRDRALLELLYGAGLRVSEAAGVDLLDLDLTNRQVRVTGKGAKTR
                     ICLFGEPAHAALTAYLERGRPHLVTGAQPALFIGREGRRLAVRSVQALVRRYGTQAAI
                     PQRVHPHLLRHTFATHMIEGDADLRIVQALLGHASADTTQIYTAVTPQRKAQLVSSAL
                     RQARDAERARGDDGAVGSAQATSRTEE"
     gene            748..1191
                     /gene="aroQ"
                     /locus_tag="PLX85_09430"
     CDS             748..1191
                     /gene="aroQ"
                     /locus_tag="PLX85_09430"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012178223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRC63424.1"
                     /translation="MRILVLNGPNLNLLGRREPEIYGLQTLADIEVLIRNRAEEHGIE
                     VDFLQSNHEGVLVDAIQEHHDWDGFIINAGALTHTSLAIADAISSVGVPAVEVHLSNV
                     HAREAFRHHSYLSPVAWGQVVGFGYRGYLAALDLLYDRLSREALG"
     gene            1188..>1687
                     /locus_tag="PLX85_09435"
     CDS             1188..>1687
                     /locus_tag="PLX85_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HRC63425.1"
                     /translation="MTNQLARLDRLRARFDALGIDALLIATPSNRRWISGFTGTAGTL
                     LVSRSQALFATDARYYEQVAAQAAAFSLVPANGPMAEWAPALLAGLGGQRVGFEPGGL
                     TYANFEEWKTVLAALPDADRPAFVPAPGAIEALRAVKEPEEVDSLTRAVLLGDAAFTH
                     AASLAHP"
BASE COUNT          267 a          619 c          552 g          249 t
ORIGIN      
        1 tcgcgccttc tacacgtggc tcgaccgcga ggcgctgctg ccgtcggcgc agcccgggga
       61 ctccattctc cgcctgcgca cgccagcggc gccccggcgg ctgccgcagt tcctgaccgc
      121 cgaggaggcg acatctctgg tcgaggcgcc gcccgccgac acggcgcggg gtctgcgtga
      181 ccgggccctg ctcgagttgc tgtacggtgc gggtctgcgg gtcagcgagg ccgcgggcgt
      241 cgacctcctc gacctcgatc tcacgaaccg ccaggtccgt gtcaccggca agggtgcgaa
      301 gacgcgcatc tgcctattcg gtgagccagc gcacgcagcg ctcacggcct acctcgaacg
      361 cgggcggcca cacctcgtca ccggagcaca gcccgccctg ttcatcggcc gcgaggggcg
      421 gcggctcgcc gttcgctccg tgcaagcgct cgtgcggaga tacggcaccc aggcggccat
      481 ccctcagcgg gtgcaccctc acctgctgcg ccacacgttt gcgacccaca tgatcgaggg
      541 cgacgccgat ctccgtatcg tgcaggcgct gctcggacac gcgagcgccg acacgacgca
      601 gatctacacg gccgtcacgc cacagcgaaa ggcgcagctc gtctcctcgg cgctccgaca
      661 ggcgcgtgac gccgagagag cgcgggggga cgacggcgcg gtaggatcgg ctcaggccac
      721 gtcacgaacg gaagaataag aagagcaatg cggatcctcg tcctgaacgg accgaatctg
      781 aaccttctcg ggcgacggga acctgagatc tacggccttc agaccctcgc ggatatcgag
      841 gtgctcatcc gcaaccgggc ggaggaacac gggatcgagg tcgacttcct gcaatcgaac
      901 cacgagggag tgctcgtcga tgcgatccag gaacaccacg actgggacgg cttcatcatc
      961 aacgccggtg cgttgaccca cacctcgctc gccatcgccg acgccatctc ctcggtgggc
     1021 gtccctgcag tagaggtgca cctctcaaac gtccacgcgc gcgaggcgtt tcgccatcac
     1081 tcctacctct cgcccgtcgc gtggggacag gtcgtcggct tcggctaccg gggctacctc
     1141 gccgcgttgg acctgctgta cgaccgcctc tcgagggaag cactgggatg accaatcagc
     1201 tcgcccgcct cgaccggcta cgcgcgcgct tcgacgcgct gggcatcgac gccttgctga
     1261 tcgcgacccc gtcgaaccgc cgctggatct ccgggttcac gggcacggcg ggcacgctcc
     1321 tcgtgagccg ctcgcaggcg ctcttcgcga ccgacgcccg atactacgaa caggtggcgg
     1381 cccaggcggc cgccttcagc ctcgtgcccg ccaatgggcc aatggcagag tgggcgcccg
     1441 ccctcctcgc cggcctcgga ggccagcgcg tcggattcga gccaggcggc ctcacctacg
     1501 ccaacttcga ggagtggaag accgtcttgg ccgccttgcc ggacgccgac cggccggcct
     1561 tcgtgccggc gccgggcgcg atcgaggcac tccgggcggt gaaagagccc gaggaagtgg
     1621 actcactcac ccgggccgtc cttctcggcg atgcggcgtt tacgcacgcg gcgagcctcg
     1681 cgcaccc
//
LOCUS       DAORPG010000481         1687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9548_length_1687_cov_3.032475, whole genome shotgun sequence.
ACCESSION   DAORPG010000481 DAORPG010000000
VERSION     DAORPG010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9548_length_1687_cov_3.032475"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PLX85_09440"
     CDS             complement(<1..887)
                     /locus_tag="PLX85_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRC63426.1"
                     /translation="MNAEGDTGAGTASLAARFVAQLRGYALILLDPSGLICGWNAGAE
                     EAFAYAADEVLGKHCSLLYPQAATTAGEPTAHLETARRDGSVWFEAALVRSDQSGFVG
                     AVTMETLRDEAGGLEGFGLIVRDVTDERHARLVMERASRQLQAIQRVTEVGLARLPFE
                     GLMAALLERVAELLVVDTVTVLLRDGDELLAVAAIGLEEQVERGVRVPLGAGFAGRVA
                     AERRRVIIDDVSRYPLVQPELRARGVRSLLGVPLMVEGDLRGVLDVGSFSGRRFTEED
                     AELLQVVADRVALALANARL"
     gene            complement(1137..>1687)
                     /locus_tag="PLX85_09445"
     CDS             complement(1137..>1687)
                     /locus_tag="PLX85_09445"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRC63427.1"
                     /translation="HPAMAPYGVFPAAGEDTFVAIAVDSDAAFAALCREMGQPELASD
                     ARFADLVSRYEHQGELEPIVAAWTKQHGQRELMERLQAAGVMATMVYNQPEMFDDPHL
                     KERGFFVELDHPDVGPKPYPGPMAQFERMPLTPLRGPAPRLGQHNREVFQGILGVSDE
                     RLAELEAAEVIGDAYLESAKAT"
BASE COUNT          264 a          659 c          518 g          246 t
ORIGIN      
        1 agccgcgcgt tcgccagcgc gagcgcgacg cggtcggcta ccacctggag cagctccgcg
       61 tcctcctcgg tgaaccgccg cccgctgaaa ctccccacgt cgaggacgcc ccgcaggtcg
      121 ccctcgacca tgagcggcac gccgagcagc gagcgcacgc ctcgagcccg cagctccggc
      181 tgcaccagcg ggtaccggct tacatcatcg atgatcacgc gacgtcgctc cgcggccacc
      241 cgcccggcga acccggctcc cagcggcacc cgcacgcccc gctcgacctg ttcttcgagc
      301 ccaatcgccg cgaccgccag cagctcgtcg ccgtctcgca gcaacaccgt caccgtatct
      361 accaccagca gttcggcgac ccgttcgagg agcgcggcca tcagcccttc gaacggcagc
      421 cgggcgaggc ccacctcggt cacacgctgg attgcctgta gctgccgcga ggcccgttcc
      481 atcaccagcc gcgcatgtcg ctcgtcggtc acgtcgcgca cgatcaaccc gaagccctca
      541 agcccgccgg cctcgtcccg cagcgtctcc atggtgacgg cgccaacaaa gccgctctgg
      601 tcgcttcgca ccagcgccgc ttcgaaccac accgaaccgt cgcgccgcgc cgtttcgagg
      661 tgcgctgtcg gctcgccggc cgtcgtcgcc gcctgcggat agagcagcga gcagtgcttg
      721 ccaaggacct cgtccgctgc gtacgcgaac gcctcctcgg cgccggcatt ccacccgcag
      781 atcaggcccg atggatcgag caggatgagc gcgtatcccc gcaactgagc gacgaagcgg
      841 gcggcgagtg acgcggtccc ggcgcctgtg tcgccctccg cgttcacgag tccgcccaca
      901 gacgtcgggc ccccgcgaaa ggcgacgccg gaaggcgcgt cgcgcgggca ggtcgagaaa
      961 gcacatcgcc tccagagcgt cggggaagtg tgacgcctga tcgatgctat cgatgccctt
     1021 gccgcgctga atctcggaac cgtcacgacg gagccaccgg acatcacagc ggcgtaacag
     1081 cgcagcggcc ctgatcggga aggggcgggc cgccccttcc cgatccatgg atcgttctag
     1141 gtcgccttcg cgctttcgag gtacgcgtcg ccgatgacct cggccgcttc gagctccgca
     1201 aggcgctcgt cgctcacccc gaggatgccc tggaacacct cgcggttgtg ctgccccagc
     1261 cggggagccg gcccccgaag cggggtcaac ggcatccgct cgaactgcgc catcggcccc
     1321 gggtacggct tcggtcccac atccgggtgg tccagctcaa cgaagaaccc ccgctccttc
     1381 aggtgtgggt cgtcgaacat ctcaggttgg ttgtacacca tcgtcgccat cacgcccgcc
     1441 gcctgcagcc gctccatcag ttcccgctgc ccgtgctgct tcgtccaggc ggccacgatc
     1501 ggttccagct cgccctggtg ctcgtacctc gacacgaggt ccgcgaaccg cgcgtccgac
     1561 gccagctccg gctgtcccat ctcccggcac agtgcagcga acgcagcgtc gctatcgacg
     1621 gcgatcgcga cgaaggtgtc ctcgccggct gcggggaaga cgccatacgg cgccatcgcc
     1681 gggtgcc
//
LOCUS       DAORPG010000482         1684 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9577_length_1684_cov_2.446900, whole genome shotgun sequence.
ACCESSION   DAORPG010000482 DAORPG010000000
VERSION     DAORPG010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9577_length_1684_cov_2.446900"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /gene="purD"
                     /locus_tag="PLX85_09450"
     CDS             <1..1106
                     /gene="purD"
                     /locus_tag="PLX85_09450"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRC63428.1"
                     /translation="RVRADLVIVAPDDPLAAGVVDALAAEGIAAFGPTKAAAELEWSK
                     SFAKQVMRDAGIPTADSRVFEDAGEARRYVEGLGGPCVVKADGLALGKGVTVCDDVAQ
                     ALAAIDAAMVGRAFGEAGARVVIEERMRGPEVSAHAFSDGVTVRHMPFSCDHKPVFDG
                     NLGPNTGGMGVYSPPGWLRPEHASFIEQEVTEATVRAMAGIGRPYRGVLYPGMMVTDS
                     GPRVVEFNCRFGDPETEVLLPRLRSDLLAICDAVAHGRLADVPVEWSEAATVGVVMAS
                     GGYPGAYETGFPIEGLDDVDGDVQVFLAGAKRDEAGMLRTSGGRVLCVVASGTTVAEA
                     RVRAYENVRRIRFEGAHYRTDIGATAEDAVHAR"
     gene            1334..1570
                     /locus_tag="PLX85_09455"
     CDS             1334..1570
                     /locus_tag="PLX85_09455"
                     /inference="COORDINATES: protein motif:HMM:NF021560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HRC63429.1"
                     /translation="MQVSYDQEADAMYVRFRPKSGRITSTRIDEQRVLDRDDQGVVGV
                     EFLFVSRGVHLEGLPDAGAIAEAIRSVPQPASAP"
BASE COUNT          249 a          501 c          671 g          263 t
ORIGIN      
        1 agcgggtgcg cgcggatctc gtgatcgtgg cgccggacga cccgctcgca gcgggcgtcg
       61 tcgatgcact cgccgccgag ggcatcgcgg ccttcgggcc gacgaaggcg gccgcggaac
      121 tcgaatggtc gaagtcgttc gcgaagcagg tgatgcgtga cgcgggcatc cccacggccg
      181 attcgcgcgt gttcgaagac gcgggcgagg cgaggcgata cgtggagggc ctcggcgggc
      241 cgtgcgtcgt caaggcggac ggcctggcgc tcggcaaggg tgtcaccgtg tgcgacgacg
      301 tcgcccaggc gctcgcggcg atcgatgcgg caatggtcgg acgcgcgttc ggggaggccg
      361 gagcgcgtgt cgtgatcgag gagcggatgc gggggcccga ggtctcggcg cacgcgttct
      421 cggacggcgt gacggtgcgg cacatgccgt tctcgtgcga ccacaagccg gtgttcgacg
      481 gcaacctcgg gccgaacacg ggggggatgg gcgtgtattc gccgcccggc tggctgcgtc
      541 ccgagcacgc ctcgttcatc gagcaggagg tgaccgaggc gaccgtgcgg gcgatggcgg
      601 gcatcggccg gccgtatcgc ggggtgctgt accccgggat gatggtgacc gactcggggc
      661 cgcgggtcgt ggagttcaac tgccgcttcg gcgacccgga gaccgaggtg ttactgcctc
      721 gactgcgatc ggatctcctc gcgatctgcg acgcggtggc gcacggccgg ctggccgacg
      781 tgccggtcga gtggagcgag gcggccacag tgggcgttgt gatggcgtcc gggggctatc
      841 cgggcgcgta cgagaccggc ttccccatcg agggcctcga tgacgtcgat ggcgacgtgc
      901 aggtgttcct ggcgggcgcg aagcgcgacg aggccgggat gctgcgcacc tcggggggcc
      961 gggtgctgtg cgtggtcgcg agcggcacga cggtggcgga ggcgcgggta cgtgcgtacg
     1021 agaacgtgcg gcgcattcgc ttcgaggggg cgcattaccg cacggatatc ggggcgacgg
     1081 cggaagatgc tgtgcatgcg agatgacctc accggtccgg ttcacaaatc acgccctcga
     1141 caagatggac gagcgcgaaa tcaccgtgga ggaggtagcg gctgtcatcg cttcgcctca
     1201 gacggtgacg gccggcgaaa ccgcggtcga atacgatgga atcgtaggtg ggcgggattg
     1261 cacgtgatcg tcgtgcgcga ctcggagccg ccgctggtga tcaccgtctg ggaaccgaat
     1321 cggtgagtca acgatgcagg tgagctacga ccaggaagcc gacgcgatgt acgtccggtt
     1381 tcgtccgaag tcgggccgga tcacctcgac gcggattgac gaacagcggg tgctggaccg
     1441 cgacgaccag ggggttgtcg gcgtcgagtt cttgttcgtg agtcgcggcg tacatctgga
     1501 ggggttgccc gatgccggcg cgatcgcgga ggcgatccgt tcggttccgc agccggcgag
     1561 tgcgccgtga cgaccgtgcg gtgcccgatg cgggagtgcc gacgctcgat cgacctcgac
     1621 gcgctgccgg cgtggccgga gcagccgtcg ccggcgccat gcctgcactt catcgcagcc
     1681 tggg
//
LOCUS       DAORPG010000483         1682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9591_length_1682_cov_3.157959, whole genome shotgun sequence.
ACCESSION   DAORPG010000483 DAORPG010000000
VERSION     DAORPG010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9591_length_1682_cov_3.157959"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..340)
                     /locus_tag="PLX85_09460"
     CDS             complement(23..340)
                     /locus_tag="PLX85_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018770439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63430.1"
                     /translation="MEWDLGIRGVALLAGMSLAFGAAAQLLFWRSTPHWLWAASAAAF
                     FVFGIMVSEWWFGWATEEDLQPNVDGLSTDEVLLIAVWPSLALVLAIRYWTLRHRAPQ
                     REK"
     gene            521..1306
                     /locus_tag="PLX85_09465"
     CDS             521..1306
                     /locus_tag="PLX85_09465"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC63431.1"
                     /translation="MSLEGRVAFVAGASRGIGADIAKHLARAGAKVAVTARTEVQVDP
                     RLPGTIHSVAAEIAAEGGVALPVVLNLRDPESIQSAVARTLEEWGRLDILVTNAAVFI
                     PGDLGSAALRHLNLSIEVNVRGRVLMMQAALPALLEAGGGHIINVSGSGSALPGPGPY
                     SAPSPGADVVFGPTESFLEHFSQAQAWALQQHNISVNMLLPSGRIRTPGNIYAENDPR
                     NPTLEFETAEDMGKAAVWIAQQPVTTLNGSILIDADVVRANNL"
     gene            complement(1316..>1682)
                     /locus_tag="PLX85_09470"
     CDS             complement(1316..>1682)
                     /locus_tag="PLX85_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC63432.1"
                     /translation="VVNLLSPGAWQYMPNYSAVGVSKAALASLTRYLAVELAPRRIRV
                     NGVSAGWVEGSDGEHSYRADISARVRPQVPAGRNVAPEDVAAAVAWLCSDESSMLVGE
                     TVHLDGGFGIAAWRDVLEG"
BASE COUNT          272 a          678 c          506 g          226 t
ORIGIN      
        1 tagccgggga acgccccccg acctacttct cccgctgcgg tgcgcggtgc ctgagcgtcc
       61 agtaccgaat cgcgagcacc agcgcaaggc tcggccagac cgcgatcagc agcacctcgt
      121 ctgtcgacag cccgtcgacg ttcggctgca ggtcctcctc ggtcgcccac ccgaaccacc
      181 actcgctgac catgatcccg aagacgaaga acgctgccgc gctcgccgcc cacagccaat
      241 gtggcgtcga gcgccagaac agcagctgcg ccgccgcccc gaacgcgagc gacatccccg
      301 cgagcagcgc gacgccgcgt atcccgagat cccattccac gaggcaccgc ctcctctcca
      361 gcgccgtcag gcgcggcccg ccggaaggcg ccacgctcga tcctcgcgag gccgcgcatc
      421 gcgcgcatgg gccgttcgct ccgcgttcac cgggacgttc gcacgtgtag ggcggtatgc
      481 tccgcctcgc gcggcccgga gccgctgagg aggatcgacc atgtcactcg aggggcgcgt
      541 cgccttcgtc gccggcgcca gccgaggcat cggcgccgac atcgcgaaac accttgctcg
      601 cgccggcgcg aaggtcgccg tcaccgcccg caccgaggtg caggtcgacc cgcgtctccc
      661 gggcaccatc cactcggtcg ccgcagaaat cgccgccgag ggcggtgtag ccctgcccgt
      721 cgtcctcaac ctccgcgacc cggagagcat ccagtcggcg gtcgcccgca ccctcgagga
      781 gtggggccgc ctcgacatcc tcgtcacgaa cgccgctgtc ttcatcccgg gcgacctcgg
      841 cagcgccgcc ctccgccacc tcaacctcag catcgaggtg aacgtgcgcg gccgcgtgct
      901 catgatgcag gccgccctcc ccgcgctcct cgaggccggc ggtggtcaca tcatcaacgt
      961 ctccggctcg ggctccgcgc tgcccggccc cggcccgtac tccgcaccat cgccgggcgc
     1021 cgacgtggtg ttcggcccca ccgagtcatt cctcgagcac ttctcacagg cgcaggcgtg
     1081 ggcgctccag caacacaaca tctcggtcaa catgctgctg cccagcggtc gcatccgcac
     1141 gcccggcaac atctacgccg agaatgaccc ccgcaacccg accctcgagt tcgaaaccgc
     1201 cgaggacatg gggaaggccg ccgtctggat cgctcagcag cccgtcacca cactgaacgg
     1261 ctcgatcctg atcgacgcgg acgtcgtccg cgcgaacaac ctctgagacg ggaagttacc
     1321 cctcgagcac gtcccgccac gccgcgatgc cgaacccgcc gtcgaggtgc acggtctccc
     1381 caaccagcat cgaggactca tccgagcaca gccacgcgac cgccgccgcc acgtcctcgg
     1441 gcgctacgtt gcggccggcc ggcacctgtg gacgcacccg tgcactgatg tccgcgcggt
     1501 acgagtgctc gccgtccgag ccttcgaccc agcccgctga cacgccgttc acccggatcc
     1561 tccgaggcgc cagctcgacc gcgagatacc gcgtgagcga ggccagcgcg gccttgctca
     1621 ccccgaccgc cgagtagttg ggcatgtatt gccaagcgcc cggcgagagc agattgacga
     1681 cg
//
LOCUS       DAORPG010000484         1674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9672_length_1674_cov_4.147622, whole genome shotgun sequence.
ACCESSION   DAORPG010000484 DAORPG010000000
VERSION     DAORPG010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9672_length_1674_cov_4.147622"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PLX85_09475"
     CDS             <1..573
                     /locus_tag="PLX85_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009359239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRC63433.1"
                     /translation="QGRNRVTACFFGEGAVAEGAFHESMNLAAVWQLPVLFCCENNLY
                     AMGTALARSESETDLVLKAAAFDVAAWPVDGMDVLAVEQHARRAVDVVRAGGGPVFLE
                     LRTYRFRAHSMYDPDRYRGREEIEHWRERDPLVTFPASMPDFLDQATLDGIERDVAAE
                     IEAAIAFAEAGSLEAAADLERFVTMDRVPS"
     gene            612..1577
                     /locus_tag="PLX85_09480"
     CDS             612..1577
                     /locus_tag="PLX85_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HRC63434.1"
                     /translation="MREAMREALLADERVFLMGEDVGAYGGCFAVSLGLFEEFGPERI
                     RDTPLSELAFVGAGIGAALGGMRPIVEIMTVNFSLLALDQILNNAATLAHMSGGQFAI
                     PLVIRIPTGGGRQLAAQHSHSLEGWYAHIPGLRVLAPATVEDARGMLAPALADPNPVL
                     IFEHVLLYNVEEEVPLEPATVDIASAAIRRGGRDVSLITYGGSLPLVLEAAAELATAG
                     IEAEVIDLRVLRPLDERTVLESVTRTHRAVVVDEGWRSGSLSAEISARIMERAFFDLD
                     APVQRICSVEVPVPYAQQLEEAARPRASEVVRVARALVEGRPVDG"
     gene            1570..>1674
                     /locus_tag="PLX85_09485"
     CDS             1570..>1674
                     /locus_tag="PLX85_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRC63435.1"
                     /translation="MADFLMPSLGADMEAGRLVEWRVKPGDRVERGDII"
BASE COUNT          242 a          556 c          612 g          264 t
ORIGIN      
        1 cagggccgca accgcgtgac ggcctgcttc ttcggcgagg gagccgtcgc cgagggcgcg
       61 ttccatgagt cgatgaacct cgcggcggtg tggcagctgc cggtgctctt ctgctgcgag
      121 aacaacctgt acgcaatggg caccgccctc gcgcggtcgg agtcggagac cgacctcgtg
      181 ctcaaggccg cggcgttcga cgtcgcggcc tggccggtcg acggcatgga cgtgctggcg
      241 gtcgagcagc atgcgcgtcg cgccgtggac gtggtgcggg caggcggcgg cccggtcttc
      301 ctcgagttac ggacctaccg cttccgggcg cattcgatgt acgacccgga ccgctaccgc
      361 ggacgcgagg agatcgagca ctggcgcgaa cgtgacccgc tcgtgacctt tcccgcgtcg
      421 atgcccgatt tcctggacca ggcgacgctg gacggcatcg aacgagatgt cgccgcggag
      481 atcgaggccg cgattgcctt cgcggaagca ggcagcctcg aggcggccgc cgacctcgag
      541 cgcttcgtca ccatggaccg ggtgccatcg tgaccgccgg tggcacccgt caactgcagt
      601 accgcgtggc gatgcgagag gcgatgcgcg aggcgctgct ggccgacgag cgcgtgttcc
      661 tcatgggaga agacgtgggc gcgtacggcg ggtgtttcgc ggtcagcctc ggcctattcg
      721 aggagttcgg gcccgaacgt atccgcgaca cgcccctctc ggagcttgcg ttcgtggggg
      781 cgggcattgg cgcggccctc ggagggatgc ggccgatcgt cgagatcatg acggtcaact
      841 tcagcctgct ggcgctggac cagatcctga acaacgccgc gacgctcgct catatgtccg
      901 gcgggcagtt cgcgatcccg ctcgtgatcc gcattccaac gggcggcggg cgccagctgg
      961 ccgcgcagca ctcgcacagc ctcgaggggt ggtacgcgca tatccccggc ctgcgggtgc
     1021 tggcgcccgc gacggtcgaa gacgcgcgcg gcatgctcgc gccagcactg gctgacccga
     1081 accccgtgct gatcttcgag cacgtgctgc tttacaacgt ggaagaggag gtgccgctcg
     1141 agcccgctac ggtcgacatc gcctcggcgg cgatacggcg cggcggacgc gacgtgtcgc
     1201 tcatcaccta cggcggcagc ctgccgctcg tgctcgaggc cgcggccgaa ctcgcgaccg
     1261 cgggcatcga ggcggaggtg atcgacctgc gcgtgctgcg gcccctggac gaacgcaccg
     1321 tcctcgagtc ggtgacgcgg actcaccggg cggtcgtcgt cgatgagggg tggcgcagcg
     1381 gcagcctgtc cgctgagatc tcggcccgca tcatggaacg tgccttcttc gacctcgacg
     1441 cgcctgtgca acgaatctgc tccgtggagg tcccggtgcc gtacgcacag cagctcgaag
     1501 aggcagcgcg gccacgcgcc tcggaggtcg tgcgcgtcgc gcgggcgctc gttgaaggga
     1561 ggccggtcga tggctgactt cctgatgccg tcactggggg cggacatgga ggcgggccgg
     1621 ctcgtcgagt ggcgcgtcaa gcccggcgac cgtgtcgagc gcggggacat catc
//
LOCUS       DAORPG010000485         1660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9795_length_1660_cov_3.274143, whole genome shotgun sequence.
ACCESSION   DAORPG010000485 DAORPG010000000
VERSION     DAORPG010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9795_length_1660_cov_3.274143"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PLX85_09490"
     CDS             complement(<1..279)
                     /locus_tag="PLX85_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63436.1"
                     /translation="MTLKEPAILEVVAPTAPSEGSPREIRRRSQQLLYVLALALAGLA
                     LAHVVLIDAFRREAPDHFRALLVIVEVIFVGLLLAAAANYFIALSRERG"
     gene            359..1399
                     /locus_tag="PLX85_09495"
     CDS             359..1399
                     /locus_tag="PLX85_09495"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRC63437.1"
                     /translation="MAGAIRVGVIGAGANTRVRHIPGLQAIAGVEVVAVCNRSEASGH
                     RVADAFGIERVTTDPEELFSADDLDAVCIGTWPYRHSEYTVRALEAGKHVLCEARMAM
                     DASEARAMLAAAKARPELVAQVVPAPFDLRSWRTVRRVLAEGALGTLREVQVTSLNGN
                     AFDLGAPLHWRERREYSGTNTMNLGILAETVERWLGPTGTVLADASVGVEERVDEDNG
                     RRVRIEIPDGIAVLARMASGVRVTYRVSTVLHAPRDPGGISLYGSRGTLHWSLDDTMT
                     LAMAGDEARPLPPDAGTEGSWRVEQDFIDSIREGKPVELTDFEAGVRYMQFTEAVWRS
                     WNERRVVEVSSV"
     gene            1449..>1660
                     /locus_tag="PLX85_09500"
     CDS             1449..>1660
                     /locus_tag="PLX85_09500"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRC63438.1"
                     /translation="MRQRGVFIRMPSSGDWDWSPRSMLLRFLINLAGLWLASEIVPGI
                     AIDDWQSLVAGTAIFAIVNTLLKPLAT"
BASE COUNT          263 a          460 c          686 g          251 t
ORIGIN      
        1 gccgcgttcg cgggagaggg cgatgaagta gtttgccgcg gcggcgagca ggaggccgac
       61 gaagatgacc tcgacgatga cgagcagggc gcggaagtgg tctggcgcct cgcggcggaa
      121 ggcgtcgatg aggacgacat gggcgagcgc gaggccggcg agggcgagcg cgaggacata
      181 gagcagttgc tgcgaccgac gacggatctc gcgggggctg ccctcggagg gtgcggtggg
      241 ggcgacgact tcgaggatcg ctggctcctt cagggtcaca gttatccacc gtccgccgta
      301 ctgtggacaa tatcagatca cttgattacg cgaactcccg cggagaaagg gcgctcgagt
      361 ggcaggtgca atccgagtcg gagtcatcgg ggcgggcgcc aacacgcgag tgcgtcacat
      421 ccccggtctg caggcgatcg cgggcgtgga ggtcgtcgcg gtgtgcaacc gcagtgaggc
      481 gagcggtcat cgggtcgccg atgcgtttgg catcgagcgg gtgacgaccg accccgagga
      541 actgttcagt gcggatgacc tcgatgcggt ctgcattggg acgtggccgt accggcacag
      601 cgagtacacg gtgcgggcgc tcgaggccgg gaagcacgtg ctgtgcgagg cgcggatggc
      661 gatggacgcg agcgaggccc gggcgatgct ggctgccgcg aaggcgcggc ccgagctcgt
      721 ggcgcaggtc gtgccggcgc cattcgacct gcggtcgtgg cggacggtgc gtcgggtgct
      781 ggccgagggc gcactgggga cgctgcgcga ggtgcaggtg acgtccctca acgggaacgc
      841 gtttgacctc ggggcgccgc tgcactggcg ggagcgtcgc gagtactccg ggacgaacac
      901 gatgaacctc ggcatcctgg cggagactgt cgagcgctgg ctcgggccga cagggacggt
      961 gctggccgac gcctcggttg gcgtggagga gcgggtcgac gaggacaacg ggcggcgggt
     1021 gcgcatcgag atcccggatg gcatcgccgt gctggcgcgg atggcgagcg gggtgcgggt
     1081 gacgtaccgg gtgagcacgg tgctgcatgc gcctcgggac ccgggcggta tctcgctgta
     1141 cgggagccgc gggacactgc actggtcgct ggacgacacc atgacactgg cgatggcggg
     1201 cgacgaggcg cggccgctgc cgccggacgc gggcaccgag gggagctggc gcgtcgagca
     1261 ggacttcatc gactcgatcc gggaggggaa gccggtcgag ctgacggact tcgaggcggg
     1321 cgtgcgatat atgcagttca cggaggcggt gtggcgctcg tggaacgagc ggcgggtcgt
     1381 ggaggtcagc tcggtctagt cagacctcga ctggtgcgga cttggacggg gcgagcgagg
     1441 agcaggcgat gcggcaacga ggcgtgttta tccggatgcc atcgagcggc gactgggact
     1501 ggtcgccgcg gtcgatgctg ctgcggttcc tgattaacct cgcggggttg tggctggcct
     1561 cggagatcgt gcccgggatc gcgatcgacg actggcaatc gctggtggcg gggacggcga
     1621 tcttcgcgat tgtgaatacg ctgctgaagc cgctcgcgac
//
LOCUS       DAORPG010000486         1656 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9834_length_1656_cov_3.988132, whole genome shotgun sequence.
ACCESSION   DAORPG010000486 DAORPG010000000
VERSION     DAORPG010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9834_length_1656_cov_3.988132"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PLX85_09505"
     CDS             complement(<1..580)
                     /locus_tag="PLX85_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006545707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63439.1"
                     /translation="MDTGDTAWMLISSALVLFMTPGLALFYGGMVRAKNALGMLLKNY
                     IAMGIVTVTWVAIGYTLAFGPDAGGGWIGNLDYWGLRHVSNVIEGDAGAIHGVAVPDT
                     VIMVFQMMFAIITPALITGAVAERMKFTTWAVFIAIWSIVVYAPMAHWVWSPDGFIFK
                     DIKAVDFAGGTVVHINAGAAALAMVFILGPRLG"
     gene            717..>1656
                     /locus_tag="PLX85_09510"
     CDS             717..>1656
                     /locus_tag="PLX85_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HRC63440.1"
                     /translation="MAAVALERRGIDTAGVTARPFLKWAGGKSQLLPELVRRSPRRIE
                     TYYEPFLGGAALFFALSSDPERAPRRAVLNDLNGQLMTTYGVVRDEPEALVAALERLQ
                     EQYACADDAGRAALYYRIRDEHRRAVGVEIAARVIFLNKTCFNGLFRVNRRGEFNVPF
                     GRYKSPRILDREGLLAASQALRGVELRSADFEGACADAGSGDFVYFDPPFHPLSATSN
                     FTAYTERDFGRAEQIRLKRCIDGLTSRGVAVMLSNSPHPWIRGGYEFAGYDIDEVQAR
                     RAINSRGDGRGPIGEILVSNQPLILALENEVEPEPVL"
BASE COUNT          270 a          506 c          587 g          293 t
ORIGIN      
        1 agccgagcct cgggccgagg atgaagacca tggcgagggc ggcggctccg gcgttgatgt
       61 ggacgacggt gccgcccgcg aagtcgacgg ccttgatgtc cttgaagatg aagccgtccg
      121 ggctccagac ccagtgcgcc atcggcgcgt agacgacgat ggaccagatc gcgatgaaga
      181 cggcccaggt ggtgaacttc atacgctcgg cgaccgcacc ggtgatcagc gcgggcgtga
      241 tgatggcgaa catcatctgg aagaccatga tgacggtgtc ggggaccgcg acgccgtgga
      301 tggcaccggc atcgccctcg atcacgttgc tgacgtgccg gaggccccag tagtcgaggt
      361 tgccgatcca gccaccaccg gcgtccgggc cgaaggcgag cgtgtagccg atcgcgaccc
      421 aggtgacggt cacgatgccc atcgcgatgt agttcttgag cagcatgccg agtgcgttct
      481 tggcccgaac catgccgccg tagaacagcg ccaggcccgg ggtcatgaac agcaccagtg
      541 ctgagctgat cagcatccag gccgtatcgc ccgtgtccat tgttcctccc tctgactcgc
      601 ctcttgtgct ttgcaagcat cgagggcgtc ggtttcacgg gctttgcggt gatatttccg
      661 tcatgtttcg tgacggttgc atttgcgttt ccgcgatggc gggtgcgagg ccctcgatgg
      721 ccgcggttgc gctggaacgg cgcggcatcg acactgcggg cgtgacagcg aggcccttcc
      781 tgaagtgggc cggcggcaaa tcgcagttgc tccccgaact ggtgaggcgc agcccgcgcc
      841 ggattgagac gtactacgag ccgttcctcg gcggggcggc gctgttcttc gcgctgtcct
      901 cggacccgga gcgtgcgccg cggcgcgcgg tgctcaacga cctcaacggg cagctcatga
      961 ccacgtacgg ggtggtgcgg gatgagcccg aggcgttggt cgccgccctc gagcggttgc
     1021 aggaacagta cgcctgcgcc gacgacgcgg ggcgcgccgc gctgtattac cgcatccgcg
     1081 atgagcatcg gcgcgcggtg ggcgtcgaga tcgcggcgcg ggtgatcttc ctgaacaaga
     1141 cctgcttcaa cgggctgttc cgggtgaacc ggcgaggcga gttcaatgtg ccgttcgggc
     1201 ggtacaagtc gcctcggatc ctcgaccggg aggggctgct ggcggcgtcg caggcactgc
     1261 gtggggtcga gctgcgatct gccgacttcg aaggcgcgtg cgcagatgcg gggtcgggcg
     1321 acttcgtgta cttcgacccc ccgttccacc cgctgtcggc gacctcgaac ttcaccgcgt
     1381 acaccgagcg ggacttcgga cgtgcggaac agattcggct caagcgatgc atcgatgggc
     1441 tgacatcgcg gggggtggcg gtgatgcttt cgaattcccc gcacccgtgg atccgcggtg
     1501 gctacgagtt cgcgggctat gacatcgacg aggtgcaggc acgacgggcg atcaactcgc
     1561 ggggtgatgg gcgcgggccg atcggcgaga ttctggtgtc caaccagccg ctcatcctag
     1621 cgttagagaa cgaagtagag ccggagccag tcctcc
//
LOCUS       DAORPG010000487         1656 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9836_length_1656_cov_3.548407, whole genome shotgun sequence.
ACCESSION   DAORPG010000487 DAORPG010000000
VERSION     DAORPG010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9836_length_1656_cov_3.548407"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /gene="purF"
                     /locus_tag="PLX85_09515"
     CDS             complement(<1..1289)
                     /gene="purF"
                     /locus_tag="PLX85_09515"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRC63441.1"
                     /translation="MREKCGVFGVFAPGEDVARLTFFGLHALQHRGQESAGIATSDGH
                     ALHLVAEMGLVTQIFDETKLEELPGHAAIGHTRYSTTGSSIACNIQPLVVEDRYPDPA
                     IVTDGNRRLALAHNGNIVNADVLRSDLEAQGVTFETTTDSEVLAQLLATAPGPTWEDR
                     FAEMMRRANGAYSLAVLTSDAVYGARDPNGVRPLCIGALNGSGYVIASETCALDQLGA
                     KFIREVEPGEVVRIDADGITSWKPIEALRPALCLLEYIYFARPDSRMRGQLLYQARMR
                     MGAALAREHPVEADIVIGVPDSATAAAIGYAQESGIPYVEALVKNRYVGRTFIQPDQR
                     LRDRGVQMKFNPLPELLAGKRVVVVDDTIVRGTTTPRVVAMLRKAGALEVHMRITSPP
                     IHDPCFYGVDMATRGELIASYKSVDEICEHIGADTLG"
     gene            complement(1363..>1656)
                     /locus_tag="PLX85_09520"
     CDS             complement(1363..>1656)
                     /locus_tag="PLX85_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRC63442.1"
                     /translation="MPVYERLQDLPEPVDIVDVFRLPWRVPPVVEDAIEAHARAVWLQ
                     LGIVNEEAAARARAAGLEVVMDRCTKIEHQHLLALEAAEPRRDAQTFGLPPRT"
BASE COUNT          270 a          483 c          630 g          273 t
ORIGIN      
        1 tagccgaggg tgtccgcgcc gatgtgctcg caaatctcgt cgaccgactt gtatgaggcg
       61 atcagctcgc cgcgggtggc catatccacg ccatagaagc aggggtcgtg gatcgggggc
      121 gaggtgatac gcatgtgcac ctcgagggcg cctgccttgc gcagcatcgc gacgacgcgg
      181 ggggtggtgg tgcctcgcac gatggtgtcg tcgacgacca cgacacgctt tcccgcgagg
      241 agctcgggca gtgggttgaa cttcatctgg acgccgcggt cgcggagacg ctggtcgggc
      301 tgaatgaagg tgcggcccac gtagcggttc ttcacgagcg cctcgacgta ggggatgccg
      361 gactcctgcg cgtagccgat ggcggcggcg gtggcggagt cgggaacgcc gatgacgata
      421 tcggcctcga cggggtgttc gcgggcgagg gccgcgccca tgcgcatgcg tgcctggtac
      481 agcagctggc cgcgcatcct cgagtccggg cgcgcgaagt agatgtactc aagcagacaa
      541 agggcgggtc tgagggcctc gatgggcttc cacgaggtga tgccatccgc atcgatgcgg
      601 acgacctcgc cgggttcgac ctcgcgaatg aacttcgcgc cgagctggtc gagggcgcag
      661 gtctcggagg cgatgacgta gccgctgccg ttgagagcgc cgatgcagag cgggcggacg
      721 ccgttggggt cgcgtgcgcc atagacggcg tcgctggtga ggaccgcgag cgagtaggcg
      781 ccgttggcgc gccgcatcat ctccgcgaag cggtcctccc acgtggggcc gggcgcggtg
      841 gcgaggagct gtgcgagcac ctcggagtct gtggtggtct cgaaggtgac gccctgcgcc
      901 tcgaggtcgg agcgcaggac gtcggcattg acgatgttgc cgttgtgggc gagggcgagg
      961 cgtcgattgc catccgtgac gatggcgggg tcggggtagc gatcctcgac gaccagcggc
     1021 tggatgttgc aggcgatgct ggagccggtg gtggagtagc gggtgtgacc gatagcggcg
     1081 tggcccggca gttcctcgag cttcgtctcg tcgaagatct gggtgacgag gcccatctcg
     1141 gcgacgaggt ggagagcatg gccatcggac gtggcaatgc ctgccgattc ctgcccacga
     1201 tgctggagtg catggagtcc aaagaaggta aggcgggcta cgtcttcacc cggtgcgaag
     1261 acaccgaaga cgccgcactt ctcacgcagg ctcgggaatt gcacccgacg ccaccccccg
     1321 gtgtgagcgc ccgctagaca tcgagtgtac gcgctgcgga agtcaggtgc gaggcggtaa
     1381 gccgaaggtt tgcgcgtccc ggcgcggctc ggcggcttcc agtgcgagca aatgctggtg
     1441 ctcgatcttt gtgcagcggt ccatcacgac ctcgaggcca gccgcacgcg cacgggcggc
     1501 ggcttcctca ttgacgatgc cgagctggag ccagacagcg cgcgcgtggg cctcgatggc
     1561 gtcttcgacg accggcggga cgcgccaggg aagacggaac acatcgacga tgtcgaccgg
     1621 ctcgggaaga tcctgcaggc gttcgtagac gggcat
//
LOCUS       DAORPG010000488         1646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9934_length_1646_cov_3.035827, whole genome shotgun sequence.
ACCESSION   DAORPG010000488 DAORPG010000000
VERSION     DAORPG010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9934_length_1646_cov_3.035827"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PLX85_09525"
     CDS             complement(<1..374)
                     /locus_tag="PLX85_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRC63443.1"
                     /translation="MPDVLDEIVEENARARAELLEAVDAIPAAVRLEPSIDGWSVKDI
                     LVNIAAWQDGYAHALELMARGERPKVPDYEDDDIDAFNARRQQESASDSWEQTLGRLR
                     AARERHEAAVRGLRVLDPDRYA"
     gene            complement(374..>1646)
                     /locus_tag="PLX85_09530"
     CDS             complement(374..>1646)
                     /locus_tag="PLX85_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRC63444.1"
                     /translation="GKVGVAISSLEDIETLTEGLPLDRVTTSMTINATGSILLALYVA
                     AAKKQGADLTKVGGTIQHDILKEYIARGTYIFPPRPSLRLVTDVFAWCKDNLPEWNTI
                     SISGYHMREAGCTAAQELAFTFADAIEYCDAALAAGLSIDDIAPRLSFFWACHSNFLE
                     EVAKFRASRRMWAKIVKERYGSTNPRSQMLRFHTQTGGATLTAQQPLNNVIRTAFQAM
                     SAVLGGTQSLHTNSWDEALALPTEGAVEIALRTQQVIGYETGVADVIDPLAGSYYIES
                     LTDRVEEEANQYLARIEDMGGALACIEQGFQQREIQESAYRLQRMTETKERVVVGVNQ
                     FQTEAVHVPEIMRVDPALAQRQIGRLQALRERRDNTAVDAALAKIRAAAHTDENMMPL
                     LIEAVECYTTLGEICNVLRQEWGEYQEVLTI"
BASE COUNT          244 a          561 c          545 g          296 t
ORIGIN      
        1 tcggcgtagc ggtccgggtc gagcacgcgc aagccacgga cggcggcctc gtgccgctcg
       61 cgcgcggcgc gcagccgccc gagcgtctgt tcccagctgt cgctcgcgga ctcctgctgc
      121 cgccgcgcgt tgaaggcgtc gatatcgtca tcctcgtagt ccggcacctt cggccgctcg
      181 ccccgcgcca tcagctcgag cgcatgcgcg taaccgtcct gccaggcagc gatgttcaca
      241 aggatgtcct tgaccgacca gccgtcgatt gagggctcca ggcggaccgc agccgggatc
      301 gcgtcaaccg cctcgagcaa ctcggctcgc gcgcgcgcgt tctcctccac gatctcgtcg
      361 aggacgtccg gcatcagatc gtgagcactt cctggtactc gccccactcc tggcgcagca
      421 cgttgcagat ctcgccgagc gtcgtgtagc actccaccgc ttcgatgagc agcggcatca
      481 tgttctcgtc ggtgtgcgcc gccgcgcgga tcttcgcgag cgcggcatcg acggccgtgt
      541 tgtcgcgtcg ctccctcagc gcctgcagcc ggccgatctg ccgctgcgcc agggccgggt
      601 cgacgcgcat gatctccggg acgtgtacgg cctccgtctg gaactggttc acgccgacga
      661 ccacgcgctc cttcgtctcg gtcatgcgct ggaggcggta ggccgactcc tggatctcgc
      721 gctgctggaa gccctgctcg atgcaggcca acgcgccgcc catgtcctcg atgcgcgcga
      781 ggtactggtt cgcctcttcc tcgacgcggt cggtcagcga ctcgatgtag tacgagccgg
      841 cgagcgggtc gatcacgtcc gcgacgcccg tctcgtaacc gatgacctgc tgcgtgcgca
      901 gggcgatctc gacggctcct tcggtgggca gcgccagcgc ctcgtcccac gagttggtgt
      961 gcagcgactg cgtgccgcca aggacggcag acattgcctg gaaagcggtg cgaatcacgt
     1021 tgttcagcgg ctgttgcgcc gtgagggtcg cgccgccggt ctgcgtatgg aaacgcagca
     1081 tctgcgaacg cggattcgtt gagccgtagc gctccttgac gatcttcgcc cacatccgcc
     1141 tcgaggcacg gaacttcgcc acctcctcga ggaagttcga gtggcaggcc cagaagaacg
     1201 agagccgcgg cgcgatatcg tcgatgctca ggcctgcggc caacgcggcg tcgcagtact
     1261 cgatcgcgtc cgcgaaggtg aacgcgagct cctgggcggc cgtgcacccg gcctcacgca
     1321 tgtggtagcc ggagatcgag atcgtgttcc actcgggcag gttgtccttg caccaggcga
     1381 agacgtccgt gacgaggcgg agcgagggcc ggggcgggaa gatgtacgtc ccgcgcgcga
     1441 tgtactcctt caggatgtcg tgctggatgg tgccgccgac cttggtcagg tccgcgccct
     1501 gcttctttgc agccgcgacg tacaacgcga gcaggattga gcccgtcgcg ttgatcgtca
     1561 tcgaggttgt gacgcggtcg agcggcaatc cctcagtgag cgtctcgatg tcctcgaggg
     1621 aagagatggc gacgccaacc ttcccg
//
LOCUS       DAORPG010000489         1646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9941_length_1646_cov_2.248900, whole genome shotgun sequence.
ACCESSION   DAORPG010000489 DAORPG010000000
VERSION     DAORPG010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9941_length_1646_cov_2.248900"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..899
                     /locus_tag="PLX85_09535"
     CDS             180..899
                     /locus_tag="PLX85_09535"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HRC63445.1"
                     /translation="MDRLPHLLATRRNQAIAAAVALIVLLASAGLVFALSSGGESDPR
                     VQNIATPASTPTAAPTPNPTPPPTPSPTPEPTPEPTPPPYDGAVARLSMPRLGINNYI
                     EPIAVIGGQMQTPTDGVYAIGWYYDYPKPGWGANSVFSAHETWNLNHGPFYALHLAVP
                     GDLIVVTMDNGIQYKYEVMTNVRYDDATIPMGEVIWPSARPRNEEWITLITCGGRIVY
                     APGSRFGEYLDRDVVQARRIE"
     gene            1011..>1646
                     /locus_tag="PLX85_09540"
     CDS             1011..>1646
                     /locus_tag="PLX85_09540"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HRC63446.1"
                     /translation="MASEEFKQLLVRLEAAQAAAPPLGDPPDFGAMRTAMEGRAFPIS
                     PGTDVHPVEVDGVRGEWVCWPGADTSRRLLLLHGGGYVMGSPATHRELAGRLSRASGC
                     AVLVLDYRLAPEAPFPAAVDDAVHALAWMRDHGPEGTSVASSMAIAGDSAGGGLALAT
                     LINVRDRGLTLPQAGLLMSPWADMLASGESYVRFGAGNIHTMADAYLAGADP"
BASE COUNT          266 a          662 c          480 g          238 t
ORIGIN      
        1 gggaggcagc ttggccgcgc gtttgctgct tcacaacggg atagctcccg caggcctccg
       61 ggcacgaaac gatccctcac ccgcgcgcac atgccgctgc cgatcacaca tccgcttgaa
      121 cgccgcgtta gcttggaagg cacgctcccc caaaccgacc acggcacgag gcaagaacgt
      181 tggatcgcct cccacacctc ctcgccaccc gtcgaaacca ggccatcgcc gccgcggtcg
      241 ccctcatcgt gctgctggca tccgcggggc tcgtattcgc gctctcgagt gggggcgagt
      301 ccgacccgcg ggtgcagaac atcgcgacgc ccgcctccac acccaccgcc gcgccgaccc
      361 ccaaccccac cccgcccccg accccgtcgc cgactccaga gccaaccccc gagcccacgc
      421 ccccgcccta cgatggcgcc gtcgcccgcc tttcaatgcc gcgactcggc atcaacaact
      481 acatcgagcc gatcgccgtg atcggggggc agatgcagac gcccaccgac ggcgtctacg
      541 ccatcggctg gtactacgac taccccaagc ccggctgggg agccaattcg gtcttctcgg
      601 cgcacgaaac ctggaacctc aaccacggcc ccttctacgc gctgcacctc gccgtacccg
      661 gcgacctcat cgtcgtcacg atggacaacg gcatccagta caaatacgag gtcatgacga
      721 acgtccgcta cgacgacgcc accattccta tgggcgaagt catctggccc agcgcccgcc
      781 cccggaacga ggaatggatc accctcatca cctgcggcgg tcgcatcgtc tatgcaccgg
      841 gttcccgctt cggtgaatac ctcgaccgcg acgtcgtgca ggccagacgc atcgagtaag
      901 cccctccgca caccacagcc gttggccgcg ccgccctcct cacggcgtcc acgcttgaaa
      961 tgcccccgcc cgaggtacaa catcccccgc agtacgggag gaactcaccc atggcgtccg
     1021 aggaattcaa gcaactgctc gtccggctcg aggcggccca ggccgctgcg ccacccctcg
     1081 gcgatccgcc ggacttcggc gccatgcgca ccgcgatgga gggccgggcg ttcccgatca
     1141 gccctggcac cgacgtacac ccggtcgagg tcgacggcgt ccgaggcgaa tgggtctgct
     1201 ggcccggcgc ggataccagc cggcgcctgc tgctcctcca tggcggcggt tatgtcatgg
     1261 gttcgccagc aacacatcgc gagctggccg gccgcctgtc ccgcgcctcc ggctgcgccg
     1321 tgctcgtcct cgactaccgc ctcgcgcccg aggcgccctt ccccgccgcg gtcgacgacg
     1381 ccgtccacgc cctggcgtgg atgcgcgacc acggccccga gggcacctcg gtcgcttcgt
     1441 cgatggcgat cgcgggtgac tcagctggcg gcgggctggc gctcgccaca ctgatcaacg
     1501 tccgggaccg tggcctgacg ctgccgcagg ccgggctcct catgtccccc tgggcggaca
     1561 tgctcgcctc cggggagtcg tacgtccgct tcggcgccgg caacatccat acgatggccg
     1621 acgcctatct cgcgggcgcc gacccg
//
LOCUS       DAORPG010000490         1644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9956_length_1644_cov_3.340466, whole genome shotgun sequence.
ACCESSION   DAORPG010000490 DAORPG010000000
VERSION     DAORPG010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9956_length_1644_cov_3.340466"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLX85_09545"
     CDS             complement(<1..312)
                     /locus_tag="PLX85_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63447.1"
                     /translation="MADEPREGRELPGATTPEDERNGGEPILIQIVDGQVELELTAAP
                     AGQVRIAAMNRGEEACDPHLGRQDAPDSTVVHLREIRPGDSAESVVELRDGFEPSEAL
                     AD"
     gene            539..769
                     /locus_tag="PLX85_09550"
     CDS             539..769
                     /locus_tag="PLX85_09550"
                     /inference="COORDINATES: protein motif:HMM:NF017353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HRC63448.1"
                     /translation="MAEEREKLEGKGQEAVGAGREKLGEMTGDERMEAEGRRQQGEGK
                     GKQMAADIKGKAEDAADAVKDKADQLGEKLGR"
     gene            complement(843..989)
                     /locus_tag="PLX85_09555"
     CDS             complement(843..989)
                     /locus_tag="PLX85_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63449.1"
                     /translation="MLALILVLVLLALLFGGLGIFVAKVFLAGLLVVLILGLLGGVAV
                     GRRT"
BASE COUNT          281 a          566 c          537 g          260 t
ORIGIN      
        1 gtcagccaac gcctcgctcg gctcgaagcc gtcccgcagc tccacgaccg actccgccga
       61 gtcgcccggc cggatttcac gcaggtgcac cacggtcgag tcgggcgcgt cctgccgtcc
      121 gagatgcggg tcgcatgcct cctcgccgcg gttcatcgcc gcgattcgta cctggccggc
      181 cggcgccgcg gtcagttcca gttcgacctg tccgtcgacg atctggatga ggatcggctc
      241 ccctccgttt cgctcgtcct ccggggtcgt cgcgccgggc agttcccggc cttctcgagg
      301 ctcgtccgcc atcgtgtggc acctcctggc tgcaactcga acgtacggcg cagtcgcctg
      361 cgcggggctg ccgtgccacg ggccggagtg tcgcgttcgt gacagcggcc cttcgccacg
      421 gtgccccgcg agtgcgcggc gtgcccgaat gaaacgcctt cgcaaccgcc gccggcccct
      481 cagccgtcgg ctgtaacgag tcgcaagcga tcctccctgc acaggaaagg agcgtggcat
      541 ggccgaagaa cgcgagaagc tcgagggcaa aggacaggag gccgtcgggg ccgggcggga
      601 gaagctcggt gagatgaccg gcgacgagcg tatggaggcc gaaggccgac gccagcaggg
      661 cgagggcaaa ggcaagcaga tggccgccga catcaagggc aaggccgagg atgcggccga
      721 cgccgtcaag gacaaggcgg atcagctggg cgagaaactc ggacgctaag cccactccgc
      781 gcgccaacaa cagggggagg ctctcagagc ctccccctgt tcacttattc cgcgaattga
      841 gctcaggtcc gccggccgac ggccacgccg ccaagcaggc cgaggatcaa taccaccagc
      901 aaccccgcga ggaacacctt cgccacgaag atgccaagcc cgccaaacaa cagcgcgagc
      961 agcaccagaa ccaggatcag ggccagcacg ttctacctct ccgctctcca gccgacatca
     1021 gtcggccctg acagggtcgg agtgagtccg acctcgcgtg gtcgcgggga gccgcgatct
     1081 ggtaacaagc ggctggcaat agtgacgtac gtcggttgat acgtcaaagt ccagatgttg
     1141 tgttgcggct gcggcgagag actcagtcac gtacccggag gatgtctgcc cccactcccg
     1201 tagtcctccg agtgcacacg gtccttcggt tgcagagtgg tcaaacgact ctcgtcggtt
     1261 ggccgaggag cgcctccctc ggccaaccga ggaccgttca ctttcgtcca gtacatcgct
     1321 gccttgcccc gcctcgttgc ctcgtccgtg cgtgctcgtt ccagcgttcg tagcgcaagg
     1381 aaacgcagtc cggcctccgc cgtcgaacgc agcgcgggcg aggctgcctc gcccgcccgt
     1441 tccttcatcc ctggaaggca tgtcccgagt atcgaggcgg ctcgttgcga cgacgtggcg
     1501 ttgtgccggc ggtcgtgtcg cttcgctgac gaggcggatc gactacagcg tgccgaggaa
     1561 ctccgggacg tcggacggcc gcagctcgat ctggtcgcca tctgcgacaa gcggcttgag
     1621 ttggatcacg ccgcgggccg cttc
//
LOCUS       DAORPG010000491         1640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_9988_length_1640_cov_3.001262, whole genome shotgun sequence.
ACCESSION   DAORPG010000491 DAORPG010000000
VERSION     DAORPG010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9988_length_1640_cov_3.001262"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..>1640)
                     /locus_tag="PLX85_09560"
     CDS             complement(170..>1640)
                     /locus_tag="PLX85_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC63450.1"
                     /translation="VALTDADEPLYTTVEMLGVQDRIAARFTKGLHRGAHLVPDGHVE
                     AALERHAHLTGEQRRLVTEWCQRGHRFQAAIGRAGAGKTTTVAACADAWTAAGYRVLG
                     AAVKGEATRTLAAATGIDCETVAWYLVHTDPQSLPLDSRTILVVDEASTLSDRDLDTL
                     MEMAATTGASLRLIGDPAQHGAIAAGGMFRVLCERHERDTPELVTTHRLQDPHDRAAA
                     QALREGRIDEAFDQLAAAGHLHIVADDLAMYREVLGRWWDAHRDGLEHPMVDRRNSTR
                     RQLNRLAHLLRRVNGELDGDEITASGDRHFAVGDRITARAPNRDLHVDGDRHAYVRNG
                     ALGTIVAVRPDRRDPADDTLTVDFDGIGTIDIPRSFFDHHRTPAGRPEVGIDHAYALT
                     SYAVQGSTRDVSTSRVDATATRSETYVDITRGRHANHLYLTASVDPLDGEALPRVPAA
                     PADLAVAERLQRSTGELTAWELAHPADQAQSVADQAVGI"
BASE COUNT          203 a          486 c          668 g          283 t
ORIGIN      
        1 cgcgtaggcc gcgttgcggc tgatccgcag gatggtggcg gcctcgtcga tggtgaggac
       61 ttcggccggg gcgtcggtct cggtggtgga catcgggtcc tcctcggtcg gtggatggtg
      121 gggtctgcct atgtaggccc cttggcgggc caggacttcg cggggattct cagattccga
      181 cggcttggtc tgccacggac tgcgcttggt cggcggggtg ggcaagttcc caggcggtga
      241 gttcgccggt ggagcgttgg aggcgttcgg cgacggcgag gtctgcgggt gcggctggga
      301 cgcgtgggag ggcttcgccg tcgagggggt cgaccgaggc ggtgaggtag aggtggttgg
      361 cgtggcgtcc gcgggtgatg tcgacgtagg tctccgagcg ggtggcggtc gcgtcgacgc
      421 ggctggtgga cacgtcgcgg gtcgaaccct gcaccgcgta ggaggtgagg gcgtaggcgt
      481 ggtcgatacc gacctcgggt cgaccagcgg gggtgcggtg atgatcgaag aacgacctcg
      541 ggatgtcgat cgtgccgatg ccgtcgaagt cgacggtcag cgtgtcgtcg gctgggtcac
      601 gtcggtcggg gcgcacggcg acgatcgtgc cgagcgcacc attgcggaca tacgcgtgcc
      661 ggtcgccgtc gacgtggagg tcgcggttgg gggcgcgggc ggtgatccgg tcgccgacgg
      721 cgaagtgtcg gtctccgctg gcggtgatct cgtcgccgtc gagttcgccg ttcacgcgtc
      781 ggaggaggtg ggcgagacgg ttgagctggc ggcgggtgct gttgcgccgg tcgaccatcg
      841 gatgctcgag cccgtcgcgg tgggcgtccc accagcggcc gagcacctcg cggtacatgg
      901 cgaggtcgtc ggcgacgatg tggaggtgac cggccgcagc gagctggtcg aacgcttcgt
      961 cgatccgacc ctcgcgcagt gcctgggccg ccgcacggtc gtgggggtct tggagccggt
     1021 gggtggtcac caactcgggg gtgtcgcgtt cgtggcgctc gcaaaggacg cggaacatgc
     1081 cccccgcagc gatggcaccg tgctgggctg ggtcgccgat cagccggagg ctcgccccgg
     1141 tggtcgcggc catctccatc aaggtgtcga ggtcacggtc cgacagcgtg gaggcttcgt
     1201 cgacgacgag gatcgtccgc gagtccagcg gcaacgactg gggatcggtg tggacgagat
     1261 accaagcgac ggtctcgcag tcgatcccgg tcgccgcggc cagggtccgc gtcgcttcgc
     1321 ccttcacggc tgcaccgagg acccggtagc cggcggcagt ccacgcatcc gcgcacgcgg
     1381 cgacggttgt ggtcttgcca gccccggcgc gtccgatggc ggcttggaag cgatgcccgc
     1441 gttggcacca ctccgtgaca agccgccgct gttcgccggt gaggtgcgcg tgccgttcga
     1501 gcgcggcctc gacgtggcca tcggggacga ggtgcgcgcc ccgatgaagt cccttggtga
     1561 agcgggccgc gatgcggtcc tgcacgccga gcatctccac ggtcgtgtag agcggttcgt
     1621 cggcatcggt gagcgcgacc
//
LOCUS       DAORPG010000492         1637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10016_length_1637_cov_3.632111, whole genome shotgun sequence.
ACCESSION   DAORPG010000492 DAORPG010000000
VERSION     DAORPG010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10016_length_1637_cov_3.632111"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLX85_09565"
     CDS             <1..409
                     /locus_tag="PLX85_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HRC63451.1"
                     /translation="LARVLGSVDVDAAVGRQMNTMLGILQSRKDSGQLADVVIIQVGN
                     NGPISKDQFEQMMELLSGAKQVVLLNVHVPLPWQDTNNAIIGDGIRHYKNATLVDWHA
                     FSEGHPELFWSDNVHLRPEGAAAYVSLIAANIR"
     gene            complement(445..1296)
                     /locus_tag="PLX85_09570"
     CDS             complement(445..1296)
                     /locus_tag="PLX85_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRC63452.1"
                     /translation="MAPPKLVDYLLYEVEESGICWIRLNRPERMNAVIGTAEENSTVV
                     KVGEYMRAADDDPRVRVIVLTGVGRAFCAGVDVRGDIPAEVIGERLVGNRNAEDTAEG
                     TRQNFIHGLTKVMRDISQVRKPTIAMINGVAAGIGMDMSLHCDIRIGCEATRFITYHQ
                     VGQIIENGGSYYLPKIAGLGRALEFAYTGHLDAERAYQWGILNHLVPSDQLEQTTREL
                     CERIIGVPPLVQWISKRIMRQALDSSLETTMVLTSNAASILGGSEDGREAREALRERR
                     TPSFRGR"
     gene            complement(1319..>1637)
                     /locus_tag="PLX85_09575"
     CDS             complement(1319..>1637)
                     /locus_tag="PLX85_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HRC63453.1"
                     /translation="GLIAAAALLGVNFVTADLRERVPWLRRAVEGNPTLLINNGQFVE
                     EHLRREHIDREEVLMALREHGLDEPSAARMAVLEVDGSISVVPAAVKPIQTRRRIRQR
                     KKN"
BASE COUNT          251 a          651 c          463 g          272 t
ORIGIN      
        1 gttggcacgc gtcctcggca gcgtggacgt cgacgccgcc gtcggccgcc agatgaacac
       61 gatgctcggc atcctccaga gccgcaaaga ctccggccag ctcgccgacg tcgtcatcat
      121 ccaggtcggc aacaacggcc ccatctcgaa ggaccagttc gagcagatga tggagctgct
      181 ctcaggcgcg aagcaggtcg tgctcctgaa cgtccacgtc ccgctcccct ggcaggacac
      241 caacaacgcg atcatcggag acggcatccg ccactacaag aacgccactc tcgtcgactg
      301 gcacgccttc agcgagggcc acccggagct cttctggagc gacaacgtcc acctgcgccc
      361 ggagggcgcg gccgcctacg tcagcctgat cgccgccaac atccgctgac cccgtccgtc
      421 gaggcgatcg cccccatcga ggccttagcg cccccggaag cttggcgtcc gccgctcccg
      481 cagtgcctcg cgcgcctcgc gcccgtcctc cgaccccccg aggatcgagg ccgcattcga
      541 ggtcagcacc atcgtcgttt ccagcgagct gtccagcgcc tgccgcatga tccgcttgct
      601 gatccactgc accagcggcg gcaccccgat gatccgttcg cacagctcgc gcgtcgtctg
      661 ctccagctgg tccgacggca ccaggtggtt caggatcccc cactggtacg cccgctccgc
      721 gtcgaggtgc cccgtgtacg cgaactccag cgcccgcccc agccccgcga tcttcggcag
      781 gtagtacgag ccgccgtttt cgatgatctg ccccacctgg tggtaggtga tgaaccgcgt
      841 cgcctcgcag ccgatgcgga tgtcgcagtg cagcgacatg tccatgccga tgcccgccgc
      901 cacgccgttg atcatcgcaa tcgtcggctt ccgcacctgc gagatgtcac gcatcacctt
      961 cgtcagcccg tggatgaagt tctgccgtgt cccctcggcg gtgtcctccg cgttgcggtt
     1021 gccgaccagc cgctccccga tcacctccgc cgggatgtcg ccacgcacat ccacgcctgc
     1081 gcagaacgcc cgccccaccc ccgtcagcac gatcacccgc acccgagggt cgtcgtcggc
     1141 cgcccgcatg tactcgccca ccttgaccac cgtactgttc tcctcggcgg tcccgatgac
     1201 ggcgttcatc cgctccggcc ggttgagccg gatccagcag atgccgctct cctccacctc
     1261 gtacagcagg taatcgacga gcttgggcgg tgccattgag gtctcctcgc agcggccgct
     1321 agttcttctt ccgctggcga atgcgccggc gcgtctggat cggcttcacc gcggccggca
     1381 ccacactaat cgaaccgtcg acttccagca cggccatccg cgccgccgag ggctcgtcga
     1441 gcccgtgctc gcgcagcgcc atcagcacct cctcgcggtc gatgtgctcc cgccgcaggt
     1501 gctcctccac gaactgcccg ttgttgatca gcagcgtcgg attgccctcc accgcccgtc
     1561 gcagccacgg cacccgctcc cgcaggtccg ccgtcacgaa gttaaccccg aggagtgccg
     1621 ccgccgcgat cagcccc
//
LOCUS       DAORPG010000493         1636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10026_length_1636_cov_4.220746, whole genome shotgun sequence.
ACCESSION   DAORPG010000493 DAORPG010000000
VERSION     DAORPG010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10026_length_1636_cov_4.220746"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1311)
                     /locus_tag="PLX85_09580"
     CDS             complement(join(101..1023,1023..1311))
                     /locus_tag="PLX85_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRC63454.1"
                     /translation="MERRGHFPKEMRERAVRMVAESAPEHPSRWAAITSVAAKLGCSA
                     ESLRKWVRQAERDSGRREGLTTSDQERLKQLERENRELKRANEILRKASAYFGPGGAR
                     PPTEMMVAFVDEHREAYGVEPICEQLPIAPATYYEHRRRQEHPEERPARVQRDQILRE
                     HVHRVWQDNQRVYGARKVWRQLRREGVEVARCTVERLMRALGLQGATRGRAFKRTTVA
                     DLGAPRPPDLVKRHFQASAPNQLWVADFTYVATWNGFVYVAFVIDVFSRRIVGWRVCA
                     SMRTDLVLDALEQAIHQRPQLEKLVHHSDRGSQYLSIRYTERLAAAGIDRSVGSIGDS
                     YDNALAESIIGLFKTEVIRTRGPWRGIEPVELATLTWVDWFNNRRLLEPLGYIPPAEF
                     EEVHYARQAAA"
     misc_feature    complement(917..1033)
                     /locus_tag="PLX85_09580"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            complement(1412..>1636)
                     /locus_tag="PLX85_09585"
     CDS             complement(1412..>1636)
                     /locus_tag="PLX85_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63455.1"
                     /translation="RRIVVSIRGTILPRRRDGMNHERIGRYLLKGALSQDQDPSIWLA
                     YDPLLDRLVDLRRVPDAHRLSTLRAETSLP"
BASE COUNT          248 a          553 c          545 g          290 t
ORIGIN      
        1 tggctcctgg tcggcagcat cctcgtgagc gagcgatgag gtccgccttc acgtgccgca
       61 ctgaagcgcc ccggcatccc cggaggctcg ttcagttgag tcaagccgcg gcctgacggg
      121 cgtaatgaac ctcttcgaac tccgcgggag gaatataccc caacggttcg aggaggcgtc
      181 ggttgttgaa ccagtcgacc catgtgagcg tggccagctc gacgggctcg atgccccgcc
      241 agggcccccg ggtgcgaatc acctccgtct tgaacaggcc gatgatcgac tcggccagcg
      301 cgttgtcgta ggagtcgccg atgctgccga cggagcggtc gatcccggcg gcggcgaggc
      361 gttcggtgta gcgaatggag aggtactgcg agccccgatc gctgtggtga acgagctttt
      421 cgagttgcgg gcgctggtgg atcgcctgct ccagtgcgtc gagcaccagg tcggtccgca
      481 tcgacgcgca gacgcgccag ccgacgatcc ggcgagagaa cacatcgatg acgaacgcga
      541 cgtagacgaa gccgttccac gttgccacgt aggtgaagtc ggccacccag agctggttgg
      601 gggccgaggc ctggaagtgc cgcttcacga ggtcgggcgg tcgcggcgcg ccgaggtcgg
      661 caacggtcgt gcgcttgaac gcgcggcctc gggtggcgcc ctggaggccc agcgcacgca
      721 tgagccgctc caccgtgcag cgcgcgactt cgacgccttc gcgacgcagt tgccgccaca
      781 ccttgcgagc gccgtagacg cgctggttgt cctgccagac gcggtggacg tgctctcgca
      841 ggatctggtc gcgctgcacc cgcgccggtc gctcctccgg gtgctcttgt cgtcgacggt
      901 gctcgtagta ggtcgccggg gcgatcggca gctgctcgca gatcggctcg accccgtacg
      961 cctcccgatg ctcgtcgacg aaggccacca tcatttcggt cggcggtcga gctccgcctg
     1021 ggcaaaatac gccgacgcct tgcgcaggat ctcgttggcc cgcttcagct cccggttttc
     1081 ccgctccagt tgcttgagcc gctcctggtc actcgtcgtc agcccctctc gacgcccgct
     1141 atcccgctcg gcctgccgca cccacttccg cagggactcc gccgaacatc ccagcttcgc
     1201 cgccaccgac gtgatcgcgg cccaccgcga cgggtgctcc ggcgcgctct ccgccaccat
     1261 ccgcaccgct cgctctcgca tctctttggg gaagtgtcct cgtctctcca tcgctccatc
     1321 ctctcaaggt ttggagtctc cggataattc ggggcgcttc agagagtagg acagacggtg
     1381 aagttgcccc gatttcgtgg acaccgctct actaaggaag cgaggtctcg gcgcgcagcg
     1441 tggagaggcg gtgcgcgtcg ggaacccggc gcagatccac caggcggtcg aggagcgggt
     1501 cgtaggccag ccagatcgac gggtcctgat cctgggagag cgcgcccttg aggaggtagc
     1561 gcccgatgcg ttcgtgattc attccgtctc gccgccgtgg caggatcgta cctcggatcg
     1621 agacgacgat gcgccg
//
LOCUS       DAORPG010000494         1635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10035_length_1635_cov_3.689241, whole genome shotgun sequence.
ACCESSION   DAORPG010000494 DAORPG010000000
VERSION     DAORPG010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10035_length_1635_cov_3.689241"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1635
                     /gene="fusA"
                     /locus_tag="PLX85_09590"
     CDS             <1..>1635
                     /gene="fusA"
                     /locus_tag="PLX85_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRC63456.1"
                     /translation="TTCSWNDHAINIIDTPGHVDFTVEVERSLRVLDGGVVVFDGVAG
                     VEPQSETVWRQADRYHVPRICFVNKMDRTGADFQRCVDMIVDRLGAVPVPLQLPIGAE
                     DRFQGVIDLIQMNAIYFEGERGAEVSVRDIPDDLRDAAEAAREALVERVAENDEQLMV
                     SFLEGHDIDITELNKAIRRATIAGDIAPVLCGSALKNKGVQAMLDAVITYLPSPVDIP
                     PVQAHPVGDDEATVERRAADDEPLTALAFKVVADPFVGRLVFFRVYSGVAKSGDQVLN
                     TTRDKRERFGRLLKMHANSREDIEAVYAGDIAAAIGLKDTFTGDTLSTREAPVMLETI
                     SFPEPVISVAIEPKTREDQEKLGDALQRLVEEDPTFRVRFDQETGQTVIAGMGELHLE
                     VIVDRMKREYKVEANIGRPQVAYRETVTRPARAEGRFVRQTGGRGQYGHCVLEIAPRE
                     PGSGFLFEDKTVGGSIPREYINPVRAGVEQALQSGVKAGFPVVDVYVSVVDGSFHPVD
                     SSEMAFTTAGSMGMRAALESGSSVLLEPVMKLEVVTP"
BASE COUNT          268 a          603 c          502 g          262 t
ORIGIN      
        1 cactacgtgc tcgtggaacg accacgccat caacatcatt gacacgccgg gccacgtgga
       61 cttcaccgtc gaggtggagc gctcgctccg cgtcctcgac ggcggtgtcg tggtcttcga
      121 cggcgtagcg ggcgttgagc ctcagtccga gacggtctgg cgtcaggccg accgctatca
      181 cgtcccgcgc atctgcttcg tcaacaagat ggaccgcacc ggcgccgact ttcagcgctg
      241 cgtcgacatg atcgtcgacc gcctcggtgc agtccccgtc cccctgcagc ttcccatcgg
      301 agccgaagac cgcttccagg gcgtcatcga cctcatccag atgaacgcca tctatttcga
      361 gggtgagcgc ggcgccgagg tgtccgtccg tgacattccg gacgacctgc gcgacgccgc
      421 agaagctgcc cgcgaggccc tcgtcgagcg cgtcgccgag aacgacgagc agctcatggt
      481 ctccttcctc gagggccatg acatcgacat caccgagctg aacaaggcca tccggcgcgc
      541 caccatcgcc ggcgacatcg ccccggtcct ctgtggctcc gccctcaaga acaagggcgt
      601 ccaggcgatg ctcgacgccg tcatcacgta cctcccctcg cccgtcgaca tcccgcccgt
      661 ccaggcgcac cccgtgggcg acgacgaagc caccgtcgag cgccgtgccg ccgacgacga
      721 gccgttgacg gcgctcgcct tcaaggtggt cgccgacccc ttcgtcggcc gcctcgtctt
      781 cttccgcgtc tactctggcg tggccaagag cggcgaccag gtgctgaaca cgacgcgcga
      841 caagcgcgag cgcttcgggc ggctgctgaa gatgcacgcc aactcccgcg aggacatcga
      901 ggccgtgtac gccggcgaca ttgccgccgc catcgggctc aaggacacct tcaccggtga
      961 caccctctcg acccgcgagg ccccggtcat gctcgagacg atctcgttcc ccgagcccgt
     1021 catctccgtc gccatcgagc ccaagactcg cgaagaccag gagaagctcg gcgatgcgct
     1081 ccagcgcctc gtcgaggagg acccgacctt ccgcgtgcgc ttcgaccagg agacgggtca
     1141 gacggtcatc gccggcatgg gtgagctcca cctcgaggtc atcgtcgacc gcatgaagcg
     1201 cgagtacaaa gtcgaggcga acatcggccg tcctcaggtc gcctaccgcg agacggtcac
     1261 ccgtcccgcc cgcgccgagg gccgcttcgt tcgccagacc ggtggtcgag gtcagtacgg
     1321 acactgcgtc ctcgagatcg ctccccgcga gcccggctcc gggttcctct tcgaggacaa
     1381 gaccgtcggc ggctccatcc cgcgcgagta catcaacccg gtccgcgcgg gcgtcgagca
     1441 ggcgctccag agcggcgtaa aggccggctt cccggtcgtc gatgtctacg tctctgtcgt
     1501 cgacgggtcc ttccacccgg tggactcgtc ggaaatggcg ttcaccacgg cgggttcgat
     1561 gggcatgcgc gccgcgcttg aaagcggcag ctccgtcctc ctcgagcccg tcatgaagct
     1621 cgaggtcgtg acgcc
//
LOCUS       DAORPG010000495         1632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10071_length_1632_cov_1.453393, whole genome shotgun sequence.
ACCESSION   DAORPG010000495 DAORPG010000000
VERSION     DAORPG010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10071_length_1632_cov_1.453393"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PLX85_09595"
     CDS             complement(<1..825)
                     /locus_tag="PLX85_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63457.1"
                     /translation="MFVLGASTLAVAAGMTAVLLHKERLSRVNLTVESQKARADAQSG
                     LEFALAALSADPAGTTWRSSSTLAFANKRYFGADASDITISLADPTDSDLTDSNLDPV
                     VVSVSAVSGQCEQNYEMTLAPAQTPLSCLDFAVTVGGALSKVGTGNIYTSGAVSVGKA
                     WEVDSDAKPSVIDALNTVALAMPVRTSPVTVFRPLAGGKTTTTTMSAQSLTVGDGSTT
                     AGGGTNSRSGAAYDPETMEAMVPNTSDTIKYYRSIGTTINFSSLSSGAIRDCVLSAS"
     gene            complement(852..1328)
                     /locus_tag="PLX85_09600"
     CDS             complement(852..1328)
                     /locus_tag="PLX85_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63458.1"
                     /translation="MLSMLVLAVLSTAALAAISQSRRARHAVDEQSMGESLARSLMSE
                     ITALPYMEASSTTIGVDAGESQGSRSTLDDVDDYNLFEESPCIMPDGKAIDGTDNWRR
                     SVVVSWVTTTANTTATGSDTGVKRIWVIVRHNNVAVARLTALRAKAWDDTLAAGGL"
     gene            complement(1394..>1632)
                     /locus_tag="PLX85_09605"
     CDS             complement(1394..>1632)
                     /locus_tag="PLX85_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63459.1"
                     /translation="ATGVAGYPARLLTSTTSGLTWTTVRDGGMACTIYGKFYGPTTAT
                     SSINRAVRATVTFEQRGGTAATSTTQLLNRPTAP"
BASE COUNT          245 a          489 c          604 g          294 t
ORIGIN      
        1 gcttgcggag agcacgcagt cccttatggc tccggacgag agggacgaga agttgatggt
       61 cgtgccgatc gaccggtagt acttgatggt gtctgaggtg ttggggacca tcgcttccat
      121 ggtctctggg tcgtacgcgg ctccggagcg ggagttggtg ccgccgcccg ccgtggttga
      181 accgtcaccg acggtcagcg actgcgcgga catggtggtt gtggtcgtct tgccgccggc
      241 caggggccgg aagaccgtaa cggggctcgt gcgcacgggc attgccagcg cgacagtgtt
      301 cagcgcatcg atgacgcttg gcttcgcgtc ggaatccacc tcccacgcct tcccgacgga
      361 gaccgccccg gaggtgtaga tgttgcccgt ccccaccttg gacagcgcgc cgcccacggt
      421 caccgcgaag tcgaggcagg agaggggcgt ctgcgcgggc gcgagcgtca tctcatagtt
      481 ctgctcgcac tggccggaga cggccgacac gctgacgacg acggggtcga ggttggaatc
      541 ggtcaggtcg ctgtcggtcg gatcggcgag cgagatcgtg atgtcgctgg cgtcggcccc
      601 gaagtagcgc ttgttcgcga aggcgagggt gctgctggac cgccatgtcg taccggcggg
      661 gtcggccgag agcgcggcga gcgcgaactc caggccggac tgtgcgtccg ccctcgcctt
      721 ctgggattcg acggtcaggt tcacacggct gaggcgctcc ttgtggagca gcacggcggt
      781 catgcccgcg gcgacggcga gcgtggatgc gcccaggacg aacacgtaga tagatcctct
      841 gcgttccatg gtcagagccc ccccgcggcg agcgtgtcgt cccaggcttt ggcgcgaagc
      901 gcagtcaggc gcgcgacggc gacgttgttg tgacgcacga tcacccagat gcgtttgacg
      961 ccggtgtccg agccggtcgc ggtggtgttc gcggtcgttg tcacccatga aaccacgacg
     1021 ctccggcgcc agttgtcggt gccgtcgatg gccttgccgt cgggcataat gcagggcgac
     1081 tcttcgaaca ggttgtagtc gtccacatcg tccagggtgc tgcgcgaacc ctgcgattct
     1141 cctgcgtcca ccccgatggt ggtggatgac gcctccatgt acgggagcgc ggtgatctcg
     1201 gacatgagcg agcgcgcgag cgactctccc atcgactgct cgtccacggc gtggcgcgct
     1261 ctgcgggatt gagagattgc cgcgagggcc gctgtcgaca gcacagccag gacgagcatc
     1321 gagagcaccg cctccacaag ggtgaggccg gcacgcgggc ggaggcgggg atggtgtcgg
     1381 tcggcggtca tggtcagggg gctgtcggac ggttcagcaa ttgggtggtc gatgtcgcgg
     1441 cagtgccgcc gcgctgctcg aaggtgacgg tggcgcggac ggccctgttg atggagcttg
     1501 tggcggttgt ggggccgtag aacttgccgt aaatagtgca ggccatcccc ccgtctcgca
     1561 ccgtcgtcca cgtcaggcca gatgtggtgg atgtcagtag gcgggcgggg tatccggcga
     1621 cgccggtagc ct
//
LOCUS       DAORPG010000496         1630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10089_length_1630_cov_2.725079, whole genome shotgun sequence.
ACCESSION   DAORPG010000496 DAORPG010000000
VERSION     DAORPG010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10089_length_1630_cov_2.725079"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..>1630
                     /locus_tag="PLX85_09610"
     CDS             32..>1630
                     /locus_tag="PLX85_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRC63460.1"
                     /translation="MPQDKITVVGAREHNLKNVTVEIPRDRLVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGLMEKPDVDHIDGLSPAISIDQKGVSKNPRSTVAT
                     VTEIYDYLRLLFARIGVPHCPHCGQIIRRQTVQQIVDAILAEPPGTRALLLAPLIRHR
                     RGEHVQIFEGVRRAGFVRVRVDGAVRDIEENIALERNKWHDIDVVVDRLIVPAPDDED
                     LDGMRQRVSDSVEQALKLGEGILLLAPEGREELTFSERYACPNTSHPPYSVGEIEPRN
                     FSFNSPHGACPACTGLGIQMELDADLVVPNKSLTIDEGAIVPYARMSTQMSWYRRRLA
                     ALAEAFGYSLDTPLKDLTELQWRTLMHGTEGETINVAYQTTRGRVRNFEVSWEGVLTN
                     LARRYRETESDWVRGDIEKYMVAIPCAECRGARLKPEMLAVTIEDRSIVDVARMSVSD
                     ASAWTERLRAEDSPLSARDRQIGRQVLQEIGGRLTFLRDVGLEYLTLDRASATLSGGE
                     GQRIRLATQIGSALMGVLYVCDEPS"
BASE COUNT          267 a          637 c          484 g          242 t
ORIGIN      
        1 tgacgacaat cgagaacatc gaggtacctc gttgccccag gacaagatca ccgtcgtcgg
       61 cgcccgcgag cacaacctca agaacgtcac cgttgagatc ccacgcgacc gcctggtcgt
      121 catcaccggg ctctccggct ccggcaagtc ctcgctcgcc ttcgacacca tctacgccga
      181 gggccagcga cggtatgtcg aatcgctttc cgcctacgcc cgccagttcc ttggcctcat
      241 ggagaagccg gacgtcgacc acatcgacgg cctctcgccc gccatctcca tcgaccagaa
      301 gggcgtctcg aagaaccctc gctccaccgt cgcgacggtc accgagatct acgactacct
      361 gcgcctgctc ttcgcccgca tcggcgtgcc gcactgcccg cactgcggcc agatcatcag
      421 gcgccagacc gtgcagcaga tcgtcgacgc catcctcgcc gagccccccg gcacgcgcgc
      481 cctgctcctc gcgccgctga tccgccaccg ccgcggcgag cacgtgcaga ttttcgaggg
      541 cgtgcgccgc gccggcttcg tgcgcgtgcg cgtcgatggc gcggtgcgcg acatcgagga
      601 gaacatcgcg ctcgagcgca acaagtggca cgacatcgac gtcgtcgtcg accgcctgat
      661 cgtccccgct cccgacgacg aggacctcga cggcatgcgc cagcgcgtct cggactccgt
      721 cgagcaggcg ctcaagctcg gcgagggcat cctcctcctc gctcccgagg gccgcgagga
      781 actgaccttc agcgagcgct acgcctgccc gaacacctcg caccccccgt actcagtggg
      841 cgagatcgag ccgcgcaact tctccttcaa cagcccccat ggcgcctgcc cggcctgcac
      901 gggcctcggc atccagatgg agctcgacgc cgacctcgtg gtgccgaaca agagcctgac
      961 catcgacgag ggcgccatcg tcccctacgc ccgcatgagc acacagatgt cgtggtaccg
     1021 ccgccgcctc gcggcgctcg ccgaggcgtt cggctactcg ctggacacgc cgctcaagga
     1081 cctgaccgag ctccaatggc gcaccctcat gcacggcacc gagggcgaga cgattaacgt
     1141 cgcctaccag accacccgcg gccgcgtccg caacttcgag gtgtcgtggg agggcgtgct
     1201 caccaacctc gcccgtcgct accgcgagac cgagtcggac tgggtgcgcg gcgacatcga
     1261 gaaatacatg gtcgccatcc cctgcgccga gtgccgcggc gcccgcctga agccggagat
     1321 gctcgccgtc accatcgagg accgctcgat cgtcgatgtc gcccgcatgt ccgtttcgga
     1381 cgcctcggca tggaccgagc gactccgcgc cgaggactcg ccgctctcgg cccgcgaccg
     1441 ccagatcggc cgccaggtgc tgcaggagat cggcggccgt ctcaccttcc tccgcgacgt
     1501 cggcctcgaa tacctgacgc tcgaccgcgc ctcggcgacg ctctccggcg gcgagggcca
     1561 gcgcatccgc ctcgccacgc agatcggctc agccctcatg ggcgtgctct atgtctgcga
     1621 cgagccgtcg
//
LOCUS       DAORPG010000497         1630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10091_length_1630_cov_2.631111, whole genome shotgun sequence.
ACCESSION   DAORPG010000497 DAORPG010000000
VERSION     DAORPG010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10091_length_1630_cov_2.631111"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PLX85_09615"
     CDS             <1..845
                     /locus_tag="PLX85_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63461.1"
                     /translation="ALDAPALHWPELGTTDAPALLRIALRDLLALGLIRRTQTDVRDV
                     SEGYRGPRGKLPQFSTPPVPAEPVAAKPVAPKPTPIAPLPTPSGTGHGASTPTPAEPA
                     PVVARPVTVEKPVVTEKPTVAPAPAPKPAPVAAPVSDPSGPVIADHLGQLTALLAAVV
                     SQGRLDPSRETVEHRSRLALARRVAVLLAAHGPLTRSDLTRMKLSTIQKDALTDAIEY
                     ALAVGAIKGGERRRFVLADATLLGLDRATVQTEVELIHQRAAERAAREANKATQAALE
                     RVTA"
     gene            913..>1630
                     /locus_tag="PLX85_09620"
     CDS             913..>1630
                     /locus_tag="PLX85_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63462.1"
                     /translation="MIESNTVETEVEDTVSDNARGLDAAEALTRRVLRSAWVTLTVCT
                     LGSAVGSVYYAYRHGSGTAAAMVSMGSVAVILWLSMHLRGGLSAARRASLMPTAAYRT
                     AATGVGVLVVIALAASYYTLHALMVAEQIAAVPAALIPAAVDVGVIVSTISIFALDRA
                     VVTVERADQREASAEQSGEARHGAVHGALVERATAPVTVPHDAPETRPTAPAKRPTER
                     SGATEPPQFEWTPEIAGREVL"
BASE COUNT          250 a          597 c          578 g          205 t
ORIGIN      
        1 tggcgctgga cgcgcccgcg ctgcactggc ccgaactggg taccaccgac gcgcccgcgc
       61 tgctgcggat cgcgctgcgc gacctgctcg cgctggggct gatccggcgc acgcagaccg
      121 acgtgcggga cgtgagcgag gggtatcgcg ggcctcgcgg gaagctcccg cagttttcga
      181 cgccgcccgt tcccgcggag ccggtggcgg cgaagcccgt cgccccgaag ccgacgccca
      241 tcgcaccact ccccactcca tccggcacgg gacacggtgc gagcaccccg acccccgccg
      301 agcccgcgcc agtcgtcgcg cgcccggtga ccgtggagaa gccggtggtc acggagaagc
      361 cgaccgtggc acccgcgccc gcgccgaagc ccgctcctgt cgccgcaccc gtctccgatc
      421 cgtccggccc cgtgatcgcc gaccacctcg gtcagctcac cgcgctgctc gcggcggtgg
      481 tctcccaagg gcggctcgat ccgtcccgcg agaccgtgga gcatcgcagc aggctcgcgc
      541 tggcacgccg ggtcgctgtg ttgctcgcag cgcacgggcc gctcacgcgc tccgatctga
      601 cgcgcatgaa gctgagcacg atccagaagg acgcactgac cgacgccatc gagtacgcgc
      661 tcgccgtcgg cgcgatcaag ggcggcgagc gccgccgttt cgtgttggcc gacgcgacgc
      721 tgcttgggct ggatcgggcg accgtccaga ccgaggtgga gctgatccac cagcgcgccg
      781 cagagcgcgc cgcgagggaa gcgaacaagg cgacgcaggc tgctctggag cgggtgacgg
      841 catgaccagc aaacacgtgt accccgcagg acgtgacggg atcggcgccc gaaggcgtcg
      901 cagggagacg ccatgatcga gagcaacacc gtagaaaccg aagtggagga caccgtgagc
      961 gacaacgcgc gaggactgga cgccgccgag gcgctgaccc gacgggtgct gcgctcagca
     1021 tgggtgaccc tgacggtctg cacgctgggc tcggctgtcg gcagcgtcta ctacgcctac
     1081 cgccacggca gcggcaccgc cgccgcgatg gtgagcatgg gctcggtcgc ggtgatcctg
     1141 tggctgtcga tgcacctgcg cggcgggctc tcggcggcgc gccgggcgtc gctcatgccg
     1201 accgccgcgt atcgcactgc ggcaaccgga gtcggcgtcc tcgtggtcat cgcactcgcc
     1261 gcgagttact acacgttgca cgcgctgatg gtcgccgagc agatcgcggc agtccccgcg
     1321 gcgctcatcc ccgcagcggt ggacgtgggc gtcatcgtca gcacgatctc gatcttcgcg
     1381 ctcgaccgcg ccgtggtgac cgtggagcgc gctgaccagc gtgaagcaag cgcggagcag
     1441 agcggtgaag cgcgccacgg agcagtgcac ggagcactgg tggagcgcgc cacggccccc
     1501 gtcaccgtgc cccacgacgc gccggagacc cgccccacgg cacccgcgaa gcggcccacg
     1561 gagcgctccg gcgcgactga gcctccccag tttgagtgga cacctgagat agcggggcgc
     1621 gaggtcctgc
//
LOCUS       DAORPG010000498         1627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10117_length_1627_cov_3.615776, whole genome shotgun sequence.
ACCESSION   DAORPG010000498 DAORPG010000000
VERSION     DAORPG010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10117_length_1627_cov_3.615776"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /gene="tatA"
                     /locus_tag="PLX85_09625"
     CDS             complement(<1..158)
                     /gene="tatA"
                     /locus_tag="PLX85_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRC63463.1"
                     /translation="MLGSLGWEELLIILVILALLFGASRVAGLGGALGRGIREFRQEA
                     SRSDSPDD"
     gene            complement(295..609)
                     /locus_tag="PLX85_09630"
     CDS             complement(295..609)
                     /locus_tag="PLX85_09630"
                     /inference="COORDINATES: protein motif:HMM:NF014662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HRC63464.1"
                     /translation="MSRELFAEIREAQQAGQPRVLATVAATRGSTPRGPGAHLLLRPD
                     GTFSGTIGGGCGEAEVWQEAMETMVDGQPRVVVVDLTEPVDGEDKICGGVMEVFIERV
                     TG"
     gene            complement(1121..>1627)
                     /locus_tag="PLX85_09635"
     CDS             complement(1121..>1627)
                     /locus_tag="PLX85_09635"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HRC63465.1"
                     /translation="RLLIVGGGHVGLSIARIGAIVGFSIAVLDDRPEFANPERFPMAE
                     EVMCGDIAEELQGTRIDNTTYIVLVSRGHMQDALALRHTVSRGPAYLGMIGSRRRTAT
                     VLQQLRDEGYSGDDLAKVHTPIGLDINAETPEEIALAILAEIVLLRKGGTGRMLSTIT
                     SRRAAEAT"
BASE COUNT          261 a          524 c          570 g          272 t
ORIGIN      
        1 tcgtcatccg gcgagtcgga gcggctggct tcctggcgga actcgcggat gccgcggccg
       61 agcgcgccgc cgaggccggc caccctcgaa gcgccgaaga gcagcgcgag gatgaccagg
      121 atgatgagca gttcttccca acccaggcta ccgagcatcg gactgtcctt tccggcgcga
      181 gtcgcgcgcc gtcagaagat cgtaacaccc cgcggcgcac ggtacagcgc acgcaacggg
      241 cgtaccagcg gtacgcccga ggagagtgcg cggattggat cggtgcgagg gagtctagcc
      301 ggtgacccgc tcgatgaaga cttccatcac accaccgcag atcttgtcct cgccatccac
      361 gggctcggtg aggtcgacga cgacgacgcg gggctggcca tcgaccatgg tctccatggc
      421 ctcctgccac acctcggcct ccccgcagcc accaccgatg gtcccgctaa aggtgccgtc
      481 cgggcggagc aggaggtgtg cgccggggcc tcgaggggtt gagccgcgcg tggcagcgac
      541 ggtggcgagg acgcggggct gcccggcctg ctgggcttca cggatttcgg cgaagagttc
      601 gcgagacatc gtcactcctg aggtgtgtgc cccctcatta tagccgtgct caccggcgtg
      661 ctgcgcaaca gatccggggg gcggcttctc gcgtatctat ggatgacacc acgaggggct
      721 gggggtgtta gatagcacaa gggccgattg ctcggccctt gtgccccaga gggatacccc
      781 cgagatgcaa ccgtgcgcaa cacctcggca ggggctggcg gtcggtcagc ctctgaggtg
      841 acttacccca tcggctaggt cagcataaca cagcggatca agcggtttac gtcgccatcg
      901 gcgacttttt ctcctcgtta cgtcttgtgg tgcgaggagt ggggcatgtc gtgcggcccg
      961 gggctttgcg cgatggactc gcattgcgaa acacccaccc gcccgctcct caaggggcgt
     1021 cgccccctga gaaacccccg gcgattctca agctggatcg cccattcgca gcgcgcggtt
     1081 gacctggcgg caccgccgcc ctctgctgcc gaggcagagc ttatgtcgct tcggccgcac
     1141 ggcggctggt gatcgtcgac agcatgcggc cagtgccacc ttttcggagc aggacgatct
     1201 ccgcgaggat cgcgagcgcg atctcctcgg gggtttcggc gttgatgtcg aggccgatcg
     1261 gggtgtggac cttcgcgagg tcgtccccgc tgtagccctc gtcgcggagc tgctggagaa
     1321 ccgtcgccgt gcggcggcgg ctgccgatca tgccgagata ggcagggccc ctcgaaacag
     1381 tgtggcggag ggcgagcgca tcctgcatgt ggccgcgcga gacgagcacg atgtaggtcg
     1441 tgttgtcgat gcgggtgccc tggagctcct cggcgatgtc gccgcacatg acttcttcgg
     1501 ccatcgggaa gcgctcgggg ttcgcgaact cgggccggtc gtcgaggaca gcgatcgaga
     1561 acccgacgat ggcgccgatg cgcgcgatcg agaggccgac gtggccgccg ccgacgatga
     1621 gcaggcg
//
LOCUS       DAORPG010000499         1626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10128_length_1626_cov_3.946531, whole genome shotgun sequence.
ACCESSION   DAORPG010000499 DAORPG010000000
VERSION     DAORPG010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10128_length_1626_cov_3.946531"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..684
                     /locus_tag="PLX85_09640"
     CDS             46..684
                     /locus_tag="PLX85_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HRC63466.1"
                     /translation="MARRLPFSVRRAEAALREADPVLAALIEAHGPFRPRPGGDPYSS
                     LVRTILFQQLAGPAARAIDRKWRALYSDDSRTPTPREILETADEQFRAAGVSRQKASY
                     LRDLALHVADGRLDFDAIGRLPDDDVIARLTDVHGLGEWSAHMFLMFELGRPDVLPTG
                     DTGMRKGMQVAYGLPEMPTPVRARDIAAPWTPYRSVGAWYMWRAVDTLTPTS"
     gene            772..1131
                     /locus_tag="PLX85_09645"
     CDS             772..1131
                     /locus_tag="PLX85_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRC63467.1"
                     /translation="MKYMLLIYSVETTREADHPDQDAMMNEYWAFTENLQKNGQYISG
                     DALQPTMTATTVRVRNGATNTSDGPFAETKEQLGGYYLVEAKDLDEAIGLAAQIPDAR
                     NGCVEVRPLMQFDMPQG"
     gene            1135..1485
                     /locus_tag="PLX85_09650"
     CDS             1135..1485
                     /locus_tag="PLX85_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRC63468.1"
                     /translation="MRYLLLVYTDERLLPQFMTPEVFEQFEELGRELYASGRLLMTGG
                     LADTSSATTVRVRDGQVLVTDGPFAETHEQLGGFSLASYDSLEQAVAMAARVPTATVG
                     CIEVRPVVEIPAPP"
     gene            1494..>1626
                     /locus_tag="PLX85_09655"
     CDS             1494..>1626
                     /locus_tag="PLX85_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-24"
                     /protein_id="HRC63469.1"
                     /translation="MTSDTIDAVFREEGGAILAALIRACGDFDLAQDALQDAVAAALE
                     "
BASE COUNT          270 a          568 c          543 g          245 t
ORIGIN      
        1 gtttcaggcc gcctcccccg gcgaggcggg ccgaggagga cagccatggc acgacggctg
       61 ccattttccg tccggcgcgc ggaagccgcg ttgcgagagg ccgatcccgt cctcgcggcc
      121 ctcatcgagg cgcacggccc gttccgcccg cgaccgggtg gcgaccccta ctcctcgctg
      181 gtccgtacga tcctgttcca gcagctcgcg gggccggccg cgcgcgcgat cgaccgcaaa
      241 tggcgggcgc tgtacagcga tgacagccgc accccgacac ctcgcgagat cctcgagacg
      301 gccgacgagc agttccgtgc cgccggcgtt tcgaggcaga aggcctcgta cctccgcgac
      361 ctggcactcc acgtcgcgga cggacgcctc gacttcgatg ccatcggtcg actgccggat
      421 gacgacgtca tcgcgcggct cacggacgtg cacggcctcg gtgagtggag cgcgcacatg
      481 ttcctgatgt tcgagctcgg gcggcccgat gtgcttccaa ccggcgacac ggggatgcgc
      541 aagggaatgc aggtcgccta tgggctgcca gagatgccca ctccggtccg ggcccgcgac
      601 atcgccgccc cgtggacgcc ctaccgctca gtcggggcct ggtacatgtg gcgagccgtc
      661 gacaccctca ccccaacttc ttgacgtcga ggtgtcgatc gggcggcccg ccgcgcgtcg
      721 tcttctcaaa ggcacgagaa cacctcgtgc gctgagcgaa gggaactgac catgaagtac
      781 atgctgctga tctactcggt cgaaacgacc cgcgaggccg accaccccga ccaggacgcg
      841 atgatgaacg agtactgggc gtttacggag aacctgcaga agaacggcca gtacatttcc
      901 ggcgacgccc tccagcccac catgaccgcg acgaccgtcc gcgtccgtaa cggcgcgacg
      961 aacacctcgg acggcccctt cgccgagacg aaggagcagc tcggcggcta ctacctcgtg
     1021 gaggcgaagg acctcgacga ggcgatcggg ttggccgcac agatcccgga cgcccgcaac
     1081 ggctgcgtcg aggtgcgccc tctcatgcag ttcgacatgc cgcaaggcta gccggtgcgg
     1141 tacctgctgc tggtctacac ggacgaacga ctgctgccgc agttcatgac gcccgaggtg
     1201 ttcgagcagt tcgaggagct cggccgggag ctgtacgcgt cggggcgtct cctcatgacc
     1261 ggaggactgg ctgacaccag ctccgccacg acagtgcgcg tccgtgacgg ccaggtgctc
     1321 gtcacggacg gtccgttcgc ggagacgcac gagcagctcg gggggttctc gctcgcctcg
     1381 tatgactcgc tggaacaggc ggtcgcgatg gcggctcgag taccgacggc gacggtcggt
     1441 tgcatcgagg tgcgaccggt cgtggagatt ccggccccgc cgtgacccag cacgtgacca
     1501 gcgacacgat cgacgccgtc ttccgcgagg aaggcggcgc catcctcgcc gcgttgatcc
     1561 gcgcctgcgg ggacttcgac ctcgcacagg atgcgctgca ggacgcggtc gccgctgcac
     1621 tcgagc
//
LOCUS       DAORPG010000500         1623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10157_length_1623_cov_3.185587, whole genome shotgun sequence.
ACCESSION   DAORPG010000500 DAORPG010000000
VERSION     DAORPG010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10157_length_1623_cov_3.185587"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="PLX85_09660"
     CDS             complement(<1..1015)
                     /locus_tag="PLX85_09660"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRC63470.1"
                     /translation="MWHAAQQLGVAGEGVRVLEPGCGSGNFIGFAPDSARVTGVELDQ
                     TTAEIAQHLYGARATIHATAFEELRVEDGAFDTVIGNVPFAKVTPHDPRHNRGRHALH
                     NYFLIKSLHLTRPGGLVVALTSRYTLDARNAAARREMASMADLVGAIRLPERAFARSS
                     GTDVVVDMVILRRRPPGAEASGPAWESVGAAPIEAAADAAPLEVNEYYLAHPEHVLGD
                     LAAARGMYRDHELTVTPTGDLDEQLPAALADLAAGAAERGLTYVAVSRDAEAHRPERR
                     AARGDFFLEHAQDGSFVVNQRGDVAQLQGGTPVTYKPRVAKDKSELVRLVGLRDAARR
                     VLAV"
     gene            complement(1366..>1623)
                     /locus_tag="PLX85_09665"
     CDS             complement(1366..>1623)
                     /locus_tag="PLX85_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63471.1"
                     /translation="GEQIAAAVSQLRDEILGPPKAGETPEEYRLRSYQALATAEELTL
                     ADHPLLQPDPSEETEDWSDEPDLARHYQDLAEINEAINTPL"
BASE COUNT          220 a          535 c          595 g          273 t
ORIGIN      
        1 gcaccgccaa cacccggcga gcagcgtcac gcaggccgac gagccgtacc agttcgctct
       61 tgtccttcgc gacacgtggc ttgtaggtga ctggggtgcc gccctggagt tgggcgacgt
      121 cgccacgctg gttgacgacg aagctgccgt cctgagcgtg ctcgaggaag aagtcgcctc
      181 gtgctgcacg tcgctccggg cggtgtgcct cggcgtcgcg gctgacagcg acgtaggtga
      241 ggccgcgctc ggccgcaccg gcggcgaggt ctgcgagcgc ggcagggagt tgctcgtcaa
      301 ggtctcccgt cggggtgacg gtcagctcgt ggtcgcgata catgccccgt gccgcagcga
      361 ggtcgccaag gacgtgttcg gggtgggcga ggtagtactc gttgacctcc aacggagccg
      421 cgtcggcggc ggcctcgatg ggcgcggcgc cgacgctctc ccacgctgga ccggaggctt
      481 cggcccccgg tggacggcga cgaaggatca ccatgtcgac gacgacgtcg gtgcccgacg
      541 agcgggcgaa ggcgcgctcc ggcagtcgga tcgcgccgac gagatcggcc atcgaagcca
      601 tctctcggcg agctgcggcg ttgcgggcat ccagggtgta gcgggaggtg agcgcgacga
      661 cgagcccgcc aggccgggtc aggtgcagcg acttgatgag gaagtagttg tgcagggcgt
      721 ggcgtccacg attgtgtcgt gggtcgtgcg gtgtgacctt ggcgaagggc acgttgccga
      781 tcaccgtgtc gaacgcgccg tcctcgacac gcaactcctc gaacgcagtg gcgtggatgg
      841 tggcccgggc tccgtagaga tgctgagcga tctcggcggt ggtctgatcg agctcgacac
      901 cggtcacgcg cgcgctgtcc ggtgcgaagc cgatgaagtt gcccgacccg cagcctggct
      961 ccaacacgcg caccccttcc cccgcgactc cgagttgctg ggcggcgtgc cacatggcct
     1021 tcacgaccgg agccgacgtg tagtgggcat tgagcgtcgt tcgccgggcc tgcgcccagc
     1081 ccgtctcagt gcgcagtagt tgccgcagct cggcgcgttc gtccgcgtag cgatcgtcag
     1141 cggtatcgaa gacctgcggg atcgcacccc aacccgacca gcgggcaagg actgcttgct
     1201 cgtcgggggt cgcccatcgc ccgccctcgt ctcggcaccg attgaggacg gtgagcgcgt
     1261 cgagattcgc tcgcagcttc gcccctgcgc ccgctggggc gaggtcatca gagctagccg
     1321 gtcggaacgg ctccagccgg gctggaggcg gggatggctc gctggctaca gcggggtgtt
     1381 gatcgcctcg ttgatctccg ccagatcctg gtagtggcgg gccaggtccg gctcgtcgct
     1441 ccagtcctcg gtctcctcgg aggggtccgg ttggagcagc gggtggtccg ccagtgtcag
     1501 ctcctcggcc gttgccaacg cctggtagct gcggagccgg tactcctccg gcgtctcgcc
     1561 ggccttgggc gggccgagga tctcgtcgcg cagctggctc accgccgccg cgatctgctc
     1621 ccc
//
LOCUS       DAORPG010000501         1623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10158_length_1623_cov_3.032526, whole genome shotgun sequence.
ACCESSION   DAORPG010000501 DAORPG010000000
VERSION     DAORPG010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10158_length_1623_cov_3.032526"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PLX85_09670"
     CDS             <1..750
                     /locus_tag="PLX85_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRC63472.1"
                     /translation="RRLALGHWRWPAFAFCGLVVLLGVGVPVGVILYWLVRGLRAGTD
                     TGFVVEAIGNSARASALAAAVAALGALPIALLSARHRGWFSGLLEKLSYTGQALPAIT
                     IALALVFFTANYTTRFYQTLTVLVIAYAVRFLPEVLGATRTALLQVNPNGEEAARSLG
                     AGPVRTFLRVTAPQMVPGVSAGMLLVFLSAVKELPITLLLSPIGFETLATDIWASTSE
                     AFFTRAALPSLILLALSGGAVLLMLRRERLS"
     gene            747..>1623
                     /locus_tag="PLX85_09675"
     CDS             747..>1623
                     /locus_tag="PLX85_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC63473.1"
                     /translation="MSEPFLRCRAVSKRFGQTQAVDRVDLEVAQGEIFALLGPSGCGK
                     TTLLRLIGGFEAPDAGEIELDGHVLASSSRFVPAEKRRIAVVFQDFALFPHMNVGKNV
                     AFGLPNGADRKRRVGELLDLVGLSGLDGRMPHELSGGQQQRVALARALAAEPRLMLLD
                     EPFSNLDPAVRQRVRAEVKQLIQSVGITAVFVTHDQEEALSLAERVAVMMDGRIVQSG
                     EPAAIYEQPATRPVAEFIGDANFLPAEVRGGIASCELGEVRVQAGFDGAAELMIRAED
                     LALRDDSASTAGVLNV"
BASE COUNT          201 a          555 c          594 g          273 t
ORIGIN      
        1 cgccgcctcg ccctcggcca ctggcgctgg ccggcgttcg cgttctgtgg cctggtggtg
       61 ctgctcggcg tcggcgtgcc cgtaggggtg atcctgtact ggctcgtccg tggcctgcga
      121 gcaggcacgg acaccggctt cgtcgtagag gcgatcggga acagcgcgcg cgcctccgcg
      181 ctcgcggcgg cggtggccgc cctcggagcg ctgccgatcg cgctgctgtc cgcacgccat
      241 cgaggctggt tcagcgggct gctcgagaag ctgtcgtaca ctggtcaggc gctgccggcg
      301 atcacgatcg cgctcgcgct ggtcttcttt acggccaact acacgacccg cttctaccag
      361 acgctgaccg tcctggtgat cgcgtacgcg gtgcggttct tgcccgaggt gctgggcgcg
      421 acccgcacgg cgctgttgca ggtcaacccg aatggtgaag aggcggcgag gagcctcggc
      481 gcgggtccgg tgcgcacgtt cctgcgcgtg accgcgccgc agatggtgcc gggcgtctcg
      541 gcggggatgc tgctcgtctt cctgtctgcg gtgaaggagc ttcccatcac cctgctgctg
      601 agcccgattg gcttcgagac gctggcgacc gacatctggg cctcgacgag cgaggcgttc
      661 ttcacgaggg ccgcgctgcc ctcgctgatc ctgctcgcgc tctcgggcgg cgccgtgttg
      721 ctcatgctgc ggcgggagcg cctctcgtga gcgagccgtt cctgcgctgc cgggccgtga
      781 gcaagcgctt tggccagaca caggccgtcg accgcgtgga cctggaggtg gcgcagggtg
      841 agatcttcgc gctgctgggg cccagcggct gcggcaagac cacgctcctg cgcctcatcg
      901 gcggcttcga ggcgcccgac gcgggtgaga tcgaactcga tggccacgtg ctggcctcgt
      961 cctcccgctt cgtgcccgcg gagaagcggc gcatcgcggt cgtgttccag gacttcgccc
     1021 tcttcccaca catgaacgtc ggcaagaacg tcgcgttcgg gctgccgaac ggcgcagacc
     1081 gcaagcgccg tgtcggcgag ctgctggacc tcgtgggcct gtccggcctc gatggccgca
     1141 tgcctcacga gctgtcgggc ggtcagcagc agcgcgtcgc cctcgcgcgc gcgctcgcgg
     1201 cggagccgcg gctgatgctg ctcgacgagc cgttctcgaa cctcgacccg gcggtgcgtc
     1261 agcgcgtgcg cgccgaggta aagcagctca ttcagtcggt cggcatcacc gcggtcttcg
     1321 tgacgcacga ccaggaggag gcgctgagcc tggccgagcg ggtcgcggtg atgatggacg
     1381 gccgcattgt gcagagcggc gagccggccg cgatctacga gcagccggcg acccgcccgg
     1441 tagccgagtt catcggcgac gcgaatttcc tcccggccga agtgcgcggc ggcatcgcca
     1501 gctgcgagct gggcgaggtg cgcgtgcagg cgggcttcga cggcgccgcc gagctgatga
     1561 ttcgcgcgga ggacctggcc ctgcgcgacg atagcgcctc gaccgccggg gtgctgaacg
     1621 tcg
//
LOCUS       DAORPG010000502         1621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10180_length_1621_cov_2.908685, whole genome shotgun sequence.
ACCESSION   DAORPG010000502 DAORPG010000000
VERSION     DAORPG010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10180_length_1621_cov_2.908685"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLX85_09680"
     CDS             <1..285
                     /locus_tag="PLX85_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRC63474.1"
                     /translation="LNEEYFYDEDGVMRNSSFLDYRMPTSLDLPMIDTILVEVPSQGH
                     PYGLRGVGEVPIVPPMATVSNAIYHATGLRMRKLPASPRVMLDELLGPEA"
     gene            493..1344
                     /locus_tag="PLX85_09685"
     CDS             493..1344
                     /locus_tag="PLX85_09685"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC63475.1"
                     /translation="MTDLHANGLRFNVHLMGEGDPVVVLIHGLVVDNLASWYFSIAPA
                     IATRAAVLLYDLRGHGRSEQPPAGYTVDDLALDLRALVTAAGLEGRPLVLAGNSTGGL
                     IALRFALRYPELVDSLVLIDAHVGQSRFGEQMAGTLELEGEERTRKLGELFGDWVDDH
                     TADGQPDADVEATLRLFRRVSTRRRNPLVSVADGLVKNTTLIADLRATPPTSDEELRS
                     LAMPILALYGEHSELRPEGERVAALAPGCVVELVPGATHGLIWQATGLLRERLGAWVD
                     ERRATKS"
     gene            complement(1331..>1621)
                     /locus_tag="PLX85_09690"
     CDS             complement(1331..>1621)
                     /locus_tag="PLX85_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63476.1"
                     /translation="APKPATVSASPANAHPGGPLTVSGSGFSPNSTVSLTVTYIAPDG
                     TQYPQPAYNVTANSAGSFSGTGILPANAYLGDYLIGAQDGDGHSASTSFTIS"
BASE COUNT          258 a          610 c          494 g          259 t
ORIGIN      
        1 ctcaacgagg agtacttcta tgacgaagac ggcgtcatgc ggaactcgag cttcctcgac
       61 taccgcatgc ccacctcgct cgacctgccc atgatcgaca ccatcctcgt cgaggtgccc
      121 agccagggcc acccctacgg cctccgaggc gtgggcgagg tcccgatcgt gccgccgatg
      181 gcgaccgtct ccaacgccat ctaccacgcg accggactgc gcatgcgcaa gctccccgcc
      241 tcgccccgcg tcatgctcga cgaactcctc ggccccgagg cgtagccccg gagtcaccat
      301 acccatagac gaagcgggct caggcagtgc cctgagcccg cttcgcaccg cccccgcggt
      361 gccgagcgaa attgcaggcc ctcgccgcag ccactcgttt gcccgggtcc ctcatccccg
      421 cgagacgagc agcccggcga cgggcatgat ggtatacgtg ggctccatcg cacccggaac
      481 gctgcccgtc gcgtgactga ccttcacgcc aatggactgc gcttcaacgt gcacctgatg
      541 ggcgagggcg atcccgtcgt ggtcctcatc catgggctcg tcgtcgacaa cctcgcgagc
      601 tggtacttca gcatcgcgcc cgcaatcgcg acccgggcgg ccgtcctgct ctacgacctg
      661 cgcggccacg gacggagcga gcaaccgccc gccggctaca ccgtcgacga tctcgcgctc
      721 gacctccgcg cccttgtcac cgccgccggt ctcgagggcc gcccgctcgt cctcgccggc
      781 aacagcaccg gagggctgat cgccctccgc ttcgcccttc gttaccccga gctggtcgac
      841 agcctcgttc tgatcgatgc gcacgtgggg caatcgcggt tcggggagca gatggccggc
      901 accctcgaac tcgagggaga ggagcgcacg aggaagctcg gcgagctctt cggcgactgg
      961 gtcgacgatc acaccgccga cggccagcct gacgccgatg tcgaagccac gctgcgcctc
     1021 tttcggcgtg tctccacccg ccgccgcaat cccctcgtct ccgtcgccga tggcctggtg
     1081 aaaaacacca cgctgattgc cgacctgcgt gcgaccccgc cgaccagcga cgaggagctc
     1141 agatcgctcg cgatgcccat cctcgcgctc tacggcgagc actccgaact ccgccccgag
     1201 ggcgaacgtg tcgctgcgct tgcacccgga tgcgtcgtcg aactcgtccc cggcgcgacg
     1261 cacggactca tctggcaggc caccggcctg ctacgcgagc gcctgggcgc ctgggtcgac
     1321 gagcgcaggg ctacgaaatc gtgaagctcg tgctcgccga gtggccgtcc ccgtcctgcg
     1381 cgccaatgag gtaatcccca aggtacgcgt tcgccggcag gattcctgta ccggagaaac
     1441 tgccggccga gttcgcggtc acgttgtacg ccggctgtgg gtattgcgtt ccgtcaggcg
     1501 cgatgtaggt gaccgtcagg ctcaccgtcg aattcggcga gaatcctgac cccgaaacag
     1561 tcagcggccc gcctgggtgt gcattcgccg gcgatgcgct caccgttgcc ggcttcggag
     1621 c
//
LOCUS       DAORPG010000503         1616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10234_length_1616_cov_3.268418, whole genome shotgun sequence.
ACCESSION   DAORPG010000503 DAORPG010000000
VERSION     DAORPG010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10234_length_1616_cov_3.268418"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLX85_09695"
     CDS             complement(<1..345)
                     /locus_tag="PLX85_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC63477.1"
                     /translation="MYPSLAGKRAVVLGGSRGIGRAIALRLAAEGVHVVVNYARSSEA
                     AEATAEECRALGVESIAVRADVGDGDELGALFEQVRAWGGLDVLVNNAARGLERPRPA
                     LEQRPKHLRATMD"
     gene            485..1507
                     /locus_tag="PLX85_09700"
     CDS             485..1507
                     /locus_tag="PLX85_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HRC63478.1"
                     /translation="MPGPHVCFDRILPRDLIRPVETRPGPGGRVRAIAPKGKAWMNGS
                     TLQVRFMGGTAAEQSVAKEQAAWWSAVANLRFDFGSHPNADIRITFDENDGAWSMIGT
                     DARQIPANQATMNLGFLDGGTAGHEFGHAIGLAHEHSSPLGGMQWNEPVVLAALAGPP
                     NFWDPDTTRHNVFFKYSMDQINGTQFDPDSIMLYAFPASWTLNGVSTHANDVLSNLDR
                     EFVAGSKMYPKSQPGLPAAATLTIGPGRAEANIGKPGEEDLYRFQVVDPGAYVIDTVG
                     PTDVYLKLFGPNNPTALIDEDDDSGLGLNPSIATQLMPGEYFAQVRHYDRTSGTGKYS
                     IGVRKR"
BASE COUNT          278 a          636 c          472 g          230 t
ORIGIN      
        1 atccatcgtc gcccgcaggt gcttcggccg ctgctccagg gctggccgcg gccgctccag
       61 gccacgcgcc gcgttgttca cgaggacgtc gaggccgccc cacgcccgca cctgctcgaa
      121 cagcgccccc agctcgtcgc cgtccccgac gtcggcccgc acggcgatcg actccacccc
      181 gagcgcgcgg cactcctcgg cagtcgcttc cgcggcctcc gaggagcgtg cgtagttcac
      241 caccacatgc acaccctcgg ccgcgagccg caacgcgatc gcgcgcccga tcccccgcga
      301 ccctccgagg acgaccgctc gcttgccagc cagtgacggg tacatccgag tcagtatcgc
      361 ccgtcgcctg acaggcagcg agcgcgaacc ttgcgctcgc acgtcatcac gtacgtgcta
      421 gcctctcatc gcgcctgccg cccgcactac ctcaaagcgg acggcgaacc gatggaggtt
      481 ccatatgcca ggaccacacg tttgcttcga ccgtatcctc ccccgcgacc tcatccgtcc
      541 ggtcgagacc cgtccggggc ccggcggccg ggtgcgagcc atcgccccga agggcaaagc
      601 atggatgaac ggctccaccc tgcaggttcg cttcatgggc ggcaccgctg ccgagcagtc
      661 cgtagcgaag gaacaggctg cctggtggtc ggccgtcgcg aacctcaggt tcgacttcgg
      721 cagccatccc aatgccgata tccgcatcac cttcgacgaa aacgacggcg cctggtcgat
      781 gatcggcacc gacgcccgcc agatccccgc caaccaggcc acgatgaacc tcgggtttct
      841 cgatggcggc accgccggac acgagttcgg ccacgccatc ggactcgccc acgagcattc
      901 gagcccgctc ggcggcatgc agtggaacga gccggtcgtc ctcgccgcgc tcgccggtcc
      961 gccgaacttc tgggaccccg acaccacccg gcacaacgtc ttcttcaagt actcgatgga
     1021 tcagatcaac ggcacccagt tcgacccgga ctcgatcatg ctgtacgcct tccccgcgag
     1081 ttggaccctg aacggcgtct cgacgcatgc gaacgacgtc ctgtcgaacc tcgaccggga
     1141 gttcgtcgcc ggctcgaaga tgtacccgaa gagccagccc ggtctgcccg cggccgcgac
     1201 cctcaccatc ggtcccgggc gcgccgaggc caacatcggc aagcccggcg aagaggacct
     1261 ctaccgcttc caggtcgtcg atcccggtgc gtacgtcatc gacacggtcg ggccgacgga
     1321 cgtctacctg aagctcttcg gccccaacaa cccgacagca ctcatcgacg aggacgatga
     1381 cagcggactc ggcctcaacc cgagcatcgc gacgcagctc atgcccggcg agtacttcgc
     1441 ccaggtccgc cactacgacc gcacgtcagg caccggcaag tactcgatcg gcgtccgcaa
     1501 gcgctgactt cgaggttccg ggcctggcga gggccgcgcc ccgcccgcta cagctgcacg
     1561 atcccgaagg cgacgccgcc cggtccgcgc actaccgcga cagaggcgcc gccctc
//
LOCUS       DAORPG010000504         1610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10303_length_1610_cov_3.902251, whole genome shotgun sequence.
ACCESSION   DAORPG010000504 DAORPG010000000
VERSION     DAORPG010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10303_length_1610_cov_3.902251"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PLX85_09705"
     CDS             complement(<1..395)
                     /locus_tag="PLX85_09705"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC63479.1"
                     /translation="MDWNDSPEQAAFRDEVRSFVKDHLPEYYDRTRLQRGFVEEAERD
                     WQWDMFHGDDERRGAAEEWAAALAERGWGAPHWPKEYGGAGLSSIEQFILRWEFAILD
                     APIIGGGGISLLGPTLLVHGTEEQKQQFL"
     gene            491..>1610
                     /locus_tag="PLX85_09710"
     CDS             491..>1610
                     /locus_tag="PLX85_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63480.1"
                     /translation="MTSTAWYGDTRRMTAGVRIRGPYLGQSGYDYHTREFTRAMVALG
                     MEVDLQQLEQWGPSGLPYELREPWLERLRRPAVVRGVVHCAFPTQVVATRWLPDINFT
                     MFEATRIPRTWVEAHRAADLVVVPTEHSRRAWIDSGVPEEKLRLCPLGVRTELFRPGV
                     RPLELEDGRGRPIGSYSRRFLNVSAVGGRKNLEGLLRAWLRATSPDDDAILLLKPGTY
                     EAGTVAELERIVQAAEEGAGKKRREAAPIEFTWLVLPDVDMPRLFALGTHYISLSFGE
                     GWDLPMSQAAAAGLRLIAPQHSAYLDYLDDEVASMIPARESPASSATDPWVNSLFEGA
                     NWWAPDEDAAVEQIRRAVAGNDTPAASARERMMERFTWR"
BASE COUNT          245 a          519 c          594 g          252 t
ORIGIN      
        1 tcaaggaact gctgcttctg ttcctcggtg ccgtgcacga ggagagtcgg gccgaggagc
       61 gagatgccac cgccgccgat gatcggggcg tcgaggatgg cgaactccca gcgcaggatg
      121 aactgctcga tgctggagag gccggcaccg ccgtactcct tgggccagtg gggcgcgccc
      181 cagccgcgct cagcgagggc ggccgcccac tcctcggcgg cgccgcggcg ttcatcgtcg
      241 ccgtggaaca tgtcccactg ccagtcgcgc tcggcttctt cgacgaaacc gcgctggagc
      301 ctcgtgcggt cgtagtactc gggcaggtgg tccttcacga acgagcgcac ctcatcgcgg
      361 aaggcggcct gctccgggct gtcgttccag tccatggcgg atcctctcat ggtcgtcgcg
      421 tcaccagtga ttacgtgcgt acgcgcgggg gtactgtacc accgggtacc gaacgggaaa
      481 cgaggagcgc gtgacctcga ccgcgtggta cggggatact cggcggatga cggccggcgt
      541 ccgcatccga ggcccctacc tgggacagag tggctacgac taccacacac gcgagttcac
      601 gcgggcgatg gtcgcactcg ggatggaggt cgacctgcag cagctcgagc agtggggccc
      661 ctcggggctg ccttacgagc tgcgtgagcc ctggctcgag cggctgagac ggcccgcggt
      721 ggtccgaggc gtggtgcact gcgcattccc gacccaggtg gtcgcgacga ggtggctgcc
      781 ggacatcaac ttcacgatgt tcgaggcaac gcgcatccct cggacgtggg tggaggcgca
      841 ccgggcggcg gacctcgtcg tcgtgccgac cgagcactcg cggcgcgctt ggatcgacag
      901 cggggtgccc gaggagaagc tgcggctgtg ccccctcggc gtgcggacgg agctgtttcg
      961 tccgggggta cgtccgctcg agctggagga cggtcggggc cgaccgatcg gttcgtacag
     1021 ccggcgattc ctgaatgtgt cggcggtcgg tgggcgcaag aacctcgagg ggttgctgcg
     1081 cgcctggctg agggctacct cacctgacga cgatgcgatc ctgctcttga agccgggcac
     1141 ctacgaggcg gggacggtgg ccgagctgga gcggatcgtg caggccgccg aggagggcgc
     1201 ggggaagaag cgccgcgagg cggcgcccat cgagttcacg tggctcgtgt tgccggacgt
     1261 cgacatgcct cggttgtttg ctctcgggac ccattacatc agcctctcgt tcggcgaagg
     1321 gtgggacctg ccgatgtcgc aggcggcggc ggccggcctg cgactgatcg ccccacagca
     1381 cagcgcctac ctcgactacc tcgatgacga ggtcgcctcg atgatcccgg cgcgcgagtc
     1441 gcccgcctcg agcgcgaccg acccgtgggt caactccctg ttcgaaggcg cgaactggtg
     1501 ggcaccggac gaggacgcag ccgtggagca gatccggcga gcggtcgccg gcaacgacac
     1561 acccgcagcc tcggcgcggg agcggatgat ggagcggttc acctggcggc
//
LOCUS       DAORPG010000505         1606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10339_length_1606_cov_3.376531, whole genome shotgun sequence.
ACCESSION   DAORPG010000505 DAORPG010000000
VERSION     DAORPG010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10339_length_1606_cov_3.376531"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /gene="nusA"
                     /locus_tag="PLX85_09715"
     CDS             complement(<1..1146)
                     /gene="nusA"
                     /locus_tag="PLX85_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRC63481.1"
                     /translation="MKNDFIIAIAQLSAEKNLDSRTVFEAVEAAMASAFKKDELQYAD
                     IEVKIDVQDGDIAAWRRYLVMPDDEIEDDEIQVSPERAREMGFPNAQLGETIREPLEQ
                     TAQTGRIAAQTAKQVVLQRLREAEREVVYDEFTSKEGELVSGTVLRVEPGRRQVIIDL
                     GRSEAVLPASEQVRAEHYRVGQRLRVYVKEVYRASKGPQVVVSRSHPDLLRRLFELEV
                     PEVARGLVEIMGVAREPGYRSKVAVISRQHGIDPIGACVGVRGSRIQNIVNELGGERI
                     DLIRWDPNPTAFVVNSLSPAEVVSIRVDEETNTAFIAVPDKQLSLAIGKEGQNARLAA
                     RLSKRRIDIQAESVLINAGQDLYPPPRPNMEAIPLAERPGLPTLPPVP"
     gene            1302..1538
                     /locus_tag="PLX85_09720"
     CDS             1302..1538
                     /locus_tag="PLX85_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63482.1"
                     /translation="MILLPSASPSRSPDDAPDEEFRTTSNPEEPHTPKDRDLLPAQQM
                     QYYDQYRSHDRWDRVLRFFLRLGESLGSKGGRWP"
BASE COUNT          241 a          614 c          482 g          269 t
ORIGIN      
        1 aggcacgggg ggcagcgtcg gcagcccggg gcgctccgcc agcgggatcg cctccatgtt
       61 cggccgcggc ggcgggtaca ggtcctgccc cgcgttgatc agcacgctct ccgcctggat
      121 gtcgatgcgg cgcttcgaaa gccgcgccgc gaggcgtgcg ttctgcccct ctttgccgat
      181 cgccaggctc agttgcttgt ccggcaccgc gatgaacgcg gtgttcgtct cctcgtcgac
      241 gcggatcgag accacttcgg ccggcgacag cgagttcacc acgaacgccg tcgggttcgg
      301 gtcccagcgg atgaggtcga tgcgctcccc gcccagctcg ttcacgatgt tctggatgcg
      361 cgacccgcgc acgcccacgc acgccccgat cgggtcgatg ccgtgctgcc gcgagatcac
      421 cgccaccttg gagcggtacc ccggctccct cgccacgccc atgatctcga ccagcccgcg
      481 cgccacctcc ggcacttcga gttcgaacag ccgccgcagc aggtccgggt gcgaccgtga
      541 cacgacgacc tgcgggccct tcgaggcgcg atacacctct ttgacgtaca cccgcagccg
      601 ctgccccacg cggtagtgct ccgcgcgcac ctgctccgac gccggcagta ccgcctcgga
      661 ccgtccgagg tcgatgatca cctgccgccg gccgggctcg acgcgcaaca cggtgcccga
      721 gaccagctcg ccttccttgc tcgtgaattc gtcgtagacg acctcgcgct ccgcctcgcg
      781 cagtcgctgc agcaccacct gctttgccgt ctgcgcggcg atgcggccgg tctgcgcggt
      841 ctgctcgagg ggttcgcgga tggtctcgcc gagctgcgcg ttcgggaacc ccatctcacg
      901 cgcccgttcc ggcgacacct ggatctcgtc gtcctcgatc tcatcgtcgg gcatcacgag
      961 gtagcggcgc cacgccgcga tgtccccgtc ctggacatcg atcttgacct cgatgtccgc
     1021 gtactgcagc tcatccttct tgaacgcgga cgccatggcc gcctcgaccg cctcgaacac
     1081 cgtccggctg tcgaggttct tctccgccga gagctgcgcg atcgcgatga tgaagtcgtt
     1141 cttcataagc ccctgtcctg gaccgcccga cgcatcgggc ccgtcccacc tgcctcccac
     1201 accgccgctt gcgaaaaaaa acgtgggccg cgcggcccac gtctcacgat ggaggcgttt
     1261 gtctccacga ataatagcac gaggagagac gcggcctcaa tgtgatactg ctgccatccg
     1321 catcgccgag tcggtccccg gacgatgcac ctgacgagga gttccgcacg acctcgaacc
     1381 ccgaagagcc gcacacaccc aaggaccgcg acctactgcc cgcgcagcag atgcagtact
     1441 acgaccagta ccgctcccac gaccgctggg accgcgtgct gcgcttcttc ctgcgcctcg
     1501 gcgaatcgct gggcagcaag ggaggccgct ggccgtgacc accgagaccg gcgtccccga
     1561 tggccgcacc ttcacatcca ctctgctgct catcgtcggc ccggcc
//
LOCUS       DAORPG010000506         1605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10345_length_1605_cov_3.000000, whole genome shotgun sequence.
ACCESSION   DAORPG010000506 DAORPG010000000
VERSION     DAORPG010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10345_length_1605_cov_3.000000"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..1436)
                     /locus_tag="PLX85_09725"
                     /pseudo
     CDS             complement(261..1436)
                     /locus_tag="PLX85_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330183.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          234 a          541 c          580 g          250 t
ORIGIN      
        1 attcggggtc agacgtcgtt gccgggggag cgccggcggg gccgtgctgc cgaccgggag
       61 ccagcgggga gcagcccgat cgacattcgg ggtcagacgt cgttaccggg ggagcgccga
      121 cggggccgtg ctgccggccg ggagccagcg gggagtcaag tcccgtttgg tggtgtattt
      181 ccgatgacgg ccggtttgcc cctacgcaga gctggccggt cgtcgtcggc gggcccgcag
      241 ggcccggtgt tggcggtgtc gcggagggcg gtgagggatt cgagggagag gtagcggtgc
      301 tggacgagcc actcgtcgtt ctgttcgatg aggatgctgg tggcgagccg caggagggcg
      361 gcgtcgttgg ggtagatgcc gatcacgtcg cttctgcggg cgagctcgag gttgacgcgt
      421 tcgagcgggt tggtgcttcg gatcttcggc cagtggtctg gggggaagcg catgtagccg
      481 agcaggtcgt cctcggcgtc gacgagcagt cgggcgatct tctcgctgct cggcgcgagc
      541 tgggtggcga cctcgctgag cgtctttcgg gcggtggggt ggtcgggggc ggcgaagatc
      601 tgcttcagcg cgccgcgcac cagcggctgg ctggtgcgcg gcacgtggcc gagcatgtcg
      661 cgcacgaagt gcacgcagca gcgctgccac tgggcgccga gcacctgggc gatggccttc
      721 ttcaggcccg ggtgggcgtc ggagatgacc agctgcgtgc cggtcagccc gcgcgccacg
      781 aggtcgcgca gaaagccgcg ccattcggcc tctgattcga cctcgccgac gccgaccgag
      841 atcacctcgc ggcggcccgt ctcgtgcacg ccgtaggcca ccagcagcgc cttctgacgc
      901 accaggccaa ggctgtcgac gcgcaccttc tcgaccttcg cgtcgagcca gagatagggg
      961 tagtcgccct ccagcggccg ctgcaggaac agctgcacgc gctcgtcgag gctgctgcag
     1021 atgcggctga cggtgctctt cgagagcgag tcgacaccga gctgctcgac cagacgctcc
     1081 accttacggg tcgagacgcc gttcacgtac gcctccgcga ccaccgccaa caaggcctgc
     1141 tcggaacgct tgcgcggctc caggaagctc gggaagtacg agccggaacg cagcttcggg
     1201 atcgccagct cgaccgtccc cacgcgcgtg ttcaacgacc gcgagcggta gccgttgcgg
     1261 tacgcgaccc gctcctcgct gcgctcgtaa cgctcgccgc cggcgaccgt cgcgacctcc
     1321 gcctccatca actcccggta gagcagcgcc accccctccc gcagcagatc ggcgtgctca
     1381 gcctccaaca gcttgcccag cgcctcgcgc agggtgacga tgccctcgtc ggccatcgcg
     1441 ctcgtacctc cctgtcgacg atcccttgcc ggatcgacat ggacggcgac agcgatggcc
     1501 gaccctcaga tcgcaccctc accccgccgc tgaaagtcca ccaagtatcg gggcacgacc
     1561 ccgacgccgg ccgcaccctg gtcgaccgga agcgggggag ggagg
//
LOCUS       DAORPG010000507         1604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10355_length_1604_cov_2.628147, whole genome shotgun sequence.
ACCESSION   DAORPG010000507 DAORPG010000000
VERSION     DAORPG010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10355_length_1604_cov_2.628147"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLX85_09730"
     CDS             complement(<1..195)
                     /locus_tag="PLX85_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63483.1"
                     /translation="MDQRLADLPPSLRYAVMAVVGLAIMLFAVGAIATAGGGDGPSDA
                     EPTSTPPVEQAASVLTATSTP"
     gene            complement(276..1079)
                     /locus_tag="PLX85_09735"
     CDS             complement(276..1079)
                     /locus_tag="PLX85_09735"
                     /inference="COORDINATES: protein motif:HMM:NF013175.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63484.1"
                     /translation="MSTMPAVAVIGFGAVGRGMQQLFPEATIYDEPLGIGTRDDVNRC
                     AVAFVCVPTPAGPDGRCDTSIVEDVVDWLDCPTIVLRSTVPPGTTERLAERTGKRIVF
                     QPEYGPGETPDHPYADPRAVRWVILGGRRAWTRPVRDLYRRVFNSDLVLRQTDARTAE
                     LTKYMENAFLALKVAFCNEFYDLAERAGVDYDELRELWLLDPRMGRSHTWVHPDERGF
                     GGKCLPKDLEALVHFGTERGEALTLLRAVLASNEAVRGRSAAIAGRRGP"
     gene            complement(1076..>1604)
                     /locus_tag="PLX85_09740"
     CDS             complement(1076..>1604)
                     /locus_tag="PLX85_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63485.1"
                     /translation="WLTSIYWLRVVAIIGSALEIIYFTYTGGDLQVAVIWTAVFIAIN
                     TFHLVLLTRERLSLRLPEAGRAVLGLLGLASIVFVTGLVQAARGGEVTLAVDPFAMHL
                     GPFEASAWLFPDYRAWTGGVVLRTVVWLIAAGVFGGWLQRDRLGDAVQWLQGRTAGIS
                     RSGARPERGRTEAVR"
BASE COUNT          267 a          567 c          555 g          215 t
ORIGIN      
        1 gggcgtcgag gtcgcggtca gcacggacgc tgcctgctcg acgggaggcg tcgaggtggg
       61 ctcggcgtcg gacggcccgt cgccgccgcc ggcggtcgcg atcgcgccga cggcaaacag
      121 catgattgcc agccccacga cggccatgac cgcgtaacgc aaggagggcg gcaggtcagc
      181 gagtcgctgg tccatgaagc gcgccgcttt cgttgccagg ggaggacttc atgctacccc
      241 agggctgcgg cggcggcacg gcgcgcctcg cgtgctcagg gacctcgccg gccggcgatg
      301 gcggcggaac ggcctcgaac cgcctcgttg gaggcgagaa cggcgcgcag cagcgtgagc
      361 gcctcgcccc gttccgtccc aaagtgcacg agcgcctcga ggtccttggg caggcacttg
      421 ccgccgaagc cgcgctcatc gggatgcacc cacgtgtggc tgcgccccat gcgagggtcg
      481 agcagccaga gctcgcgcag ctcgtcgtag tccacgccgg cgcgctcggc gaggtcgtag
      541 aactcgttgc agaaggcgac cttcagcgcg aggaaggcgt tctccatgta cttcgtcagc
      601 tcggctgtcc gcgcgtccgt ctgccgcagc acgaggtccg agttgaacac gcgtcggtac
      661 aggtcacgga ccggccgcgt ccaggcgcgc cggccgccga ggatgaccca gcgcacggcg
      721 cgcgggtcgg cgtacgggtg gtctggcgtc tcgcctggcc cgtactccgg ctggaagaca
      781 atgcgcttcc ccgtccgctc cgccagccgt tcggtcgtcc cgggcggcac ggtggagcgc
      841 aggacgatcg tcgggcaatc gagccagtca acgacgtcct cgacgatcga ggtgtcgcag
      901 cgtccgtcgg ggccggcggg tgtgggaacg cacacgaagg cgacggcgca gcgattcacg
      961 tcatcgcgcg tcccgatccc gagtggctcg tcatagatcg tcgcttcggg gaagagctgc
     1021 tgcatcccgc gccccacggc gccgaaccca atcaccgcca ccgctggcat cgtgctcatc
     1081 gcaccgcctc cgtccggccg cgctcagggc gcgcgccact ccttgagatc cctgcggtcc
     1141 ggccctgcag ccactggacc gcatcgccca gccggtcacg ctgcagccag ccgccgaaga
     1201 cgcccgcggc gatgagccag acgaccgtcc gtaggaccac gccgcccgtc caggcgcggt
     1261 agtcaggaaa cagccacgcg gacgcctcga acggtcccaa gtgcatcgcg aaggggtcga
     1321 ccgcgagcgt gacttcgccc ccgcgggccg cctgcaccag gccggtgacg aagacgatcg
     1381 aggccaatcc cagcagcccg aggaccgcac ggcccgcctc cggcaggcgc aggctcaggc
     1441 gctcgcgggt caaaagcacc aggtggaacg tgttgatggc aatgaaaacg gcggtccaga
     1501 tgaccgccac ctgcaaatcg ccgccagtgt aggtgaaata gatgatttcc agcgctgacc
     1561 cgatgatggc gacaacgcgc agccaataga tgctggtcag ccaa
//
LOCUS       DAORPG010000508         1602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10368_length_1602_cov_2.539108, whole genome shotgun sequence.
ACCESSION   DAORPG010000508 DAORPG010000000
VERSION     DAORPG010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10368_length_1602_cov_2.539108"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLX85_09745"
     CDS             complement(<1..292)
                     /locus_tag="PLX85_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63486.1"
                     /translation="MRTPLHEWRDRPGAIEAVEEQAEQRGRQRGLVAAKKLYESGVED
                     AVMEWADVSMPTNLGAGTTGSNFTSYAGVGVRVLRAGRHGLIASYRLHHISNG"
     gene            299..508
                     /locus_tag="PLX85_09750"
     CDS             299..508
                     /locus_tag="PLX85_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63487.1"
                     /translation="MSAVRREIRGELEAAFAGGERALRALTQIDGAALLDEATWRTAD
                     PDARSFVGVNTPEDLARAEAMLAAP"
     gene            complement(505..1137)
                     /locus_tag="PLX85_09755"
     CDS             complement(505..1137)
                     /locus_tag="PLX85_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC63488.1"
                     /translation="MASDPLRVLVWAAFPALRAGISQLLRREGLATCELVSLDALQDG
                     GAPGDVLAADVEDAASALEVLRTGGSLELPTLLMAPDDLVPPDSVFEADRGVGWVARS
                     ASPADLAAALRAVAAGFLVTEPGLVRPLLSADQDEQSMQAASATSLTPREVEVLQWLA
                     LGLPNKTIALRLGISEHTVKFHVGSVLSKLDAASRTEAVTTAARRGLLAL"
     gene            complement(1149..>1602)
                     /locus_tag="PLX85_09760"
     CDS             complement(1149..>1602)
                     /locus_tag="PLX85_09760"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRC63489.1"
                     /translation="PLVDVDGRVLGVNVSLIGGPRMFRFARAAFGQGFTVDAASVSRT
                     VELLKTHGRVRRAYLGIASQPVTVPTAGGAEPTEGLLIVSVEADTPAAQAGVLVGDIL
                     VGIEGAPVRDADDLQRALSPERVGMTVQLQLIRGGAAHELAVTLGARE"
BASE COUNT          227 a          587 c          566 g          222 t
ORIGIN      
        1 tgccgttcga gatgtggtgc agccggtagc tggcgatgag gccgtggcgc ccggcgcgca
       61 gcacccgcac gccgacgccc gcgtacgagg tgaagttgct gccggtggtg ccggcgccga
      121 ggttggtcgg catcgacacg tcggcccact ccatcaccgc gtcctccacg cccgattcgt
      181 agagcttctt cgctgcgacc agcccgcgct ggcgcccgcg ctgctccgcc tgctcctcga
      241 cggcctcgat ggcgcccggt cggtcgcgcc actcgtggag gggcgtgcgc aaccactggt
      301 gagcgcggtc cgtcgcgaga ttcgaggcga gctggaggcc gcgttcgccg gaggcgaacg
      361 cgcgctccgc gctctgacgc agatcgatgg cgcagcgttg ctcgacgagg ccacctggcg
      421 taccgcggac ccggatgctc gctcgttcgt cggcgtgaat acaccggaag acctggcacg
      481 cgccgaggcg atgctcgcgg cgccctagag tgcgagcagt ccccggcgcg cggccgtcgt
      541 cacggcctcg gtgcgtgacg ctgcgtcgag ctttgagagc accgagccca cgtggaactt
      601 caccgtgtgc tcactgatgc ccagccgcag cgcgatcgtc ttgttcggca gtccgagcgc
      661 aagccactgc agcacctcga cttcgcgcgg cgtcagcgag gtggcgctgg cggcctgcat
      721 cgactgctcg tcctggtcgg cgctgagtaa cggccggacg agcccgggct ccgtcacgag
      781 gaaccccgca gcgacggccc gcaacgccgc agcgaggtcg gccggcgatg ccgaacgcgc
      841 cacccatccc accccgcggt cggcctcgaa gacgctgtcc gggggcacaa ggtcatcggg
      901 cgccatcagg agcgtcggca gttcgagtga cccgccggtg cgcaacacct cgagcgcgct
      961 ggcggcatcc tcgacgtcgg ccgccagcac gtctccgggc gcgccgccgt cctgaagcgc
     1021 gtcgagcgag acgagctcgc aggtggcgag gccctcccgg cgcaggagct ggctgatgcc
     1081 ggcccgcaac gcggggaagg ccgcccagac gaggactcgc aaggggtcgg acgccaccgc
     1141 gcgctccgct actcgcgtgc tccaagcgtg acggccagct cgtgtgccgc cccgcctcgg
     1201 atcaactgta gctggacagt catgcccacg cgttcgggac tgagcgcgcg ctgcaggtca
     1261 tcggcgtcgc gcacgggcgc cccctcgatg cccacgagga tgtcgcccac gaggacgccg
     1321 gcctgcgcgg ccggggtgtc ggcctcgacg ctgacgatga gcagtccctc tgttggctcc
     1381 gcgcccccgg cggtgggtac ggtcaccggc tgactcgcga tgccgagata cgcgcgccgc
     1441 actcgcccgt gggtcttcag cagctccacc gtgcgcgaaa cggatgccgc gtcgacggtg
     1501 aagccctggc cgaaggctgc acgcgcgaag cggaacatgc gcgggccgcc gatgagcgat
     1561 acgttcacgc ccagtacccg cccgtcgacg tccacgagcg gc
//
LOCUS       DAORPG010000509         1596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10429_length_1596_cov_2.545750, whole genome shotgun sequence.
ACCESSION   DAORPG010000509 DAORPG010000000
VERSION     DAORPG010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10429_length_1596_cov_2.545750"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..>1596
                     /locus_tag="PLX85_09765"
     CDS             30..>1596
                     /locus_tag="PLX85_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRC63490.1"
                     /translation="MTEGAPILHDDLRTAEMISLFEPPAEGTGHATNVASHMRLAQGD
                     IAAGFAEADIVLEREFETSMAHQGYIEPHNATVLWNKDDDITVWTSTQGPFSVRSSLA
                     ALLDHPVSKIRVMPMEIGGGFGGKIPIYLEPFAALLSKKTGGAPIKMWMTREEVLEGT
                     GPTSGTYMRAKLGAKRDGTIVAAEVYLAFEAGAFPGSPVGAGVMCAVAPYTIPNVQVD
                     GYDIVVNRPKVAAYRGPGAPQAEFAVESVIDELAEQLEMDPIDLRLKNAAVEGTRRAD
                     GVVFGPIGNIAVLQAIKDSPHYRSELQGENRGRGVAIGFWFNVGFESAAYASVNVDGT
                     VALSTGSPDIGGTRATHAMQLAEALGIPYEDVKPHVVDTDSIGYTNVTGGSRTAFAGG
                     WAAYEAAMDIARQLEARAALIWDVDPGTVRYADGVVSGPNGKSMTFKEIAAQLPHTGG
                     MVQGRASVNPTEVGPSFAGHICDVEVDPDTGKVQVLRYTAVQDVGRAVHPSYVEGQMQ
                     GGASQGIGMALNEE"
BASE COUNT          265 a          624 c          479 g          228 t
ORIGIN      
        1 tctcccccgt catgcacgtc cgcgatgcca tgacagaggg tgccccgatc ctccatgacg
       61 acctccgcac ggccgagatg atcagcctct tcgaaccgcc cgccgagggc accggccacg
      121 ccaccaacgt cgccagccac atgcgtctcg cgcagggcga catcgccgct ggcttcgccg
      181 aggccgacat cgtcctcgag cgcgagttcg agacctcgat ggcccatcag ggctacatcg
      241 agccccacaa cgccaccgtg ctctggaaca aggacgacga catcaccgtc tggaccagca
      301 cacagggccc cttctccgtg cgctcctcgc tcgctgccct gctcgaccac ccggtctcga
      361 agatccgcgt catgccgatg gagatcggcg gcggctttgg cggcaaaatc ccgatctacc
      421 tcgagccctt cgccgcgctg ctctcgaaga agaccggcgg cgcgcccatc aagatgtgga
      481 tgacccgcga ggaagtcctt gaaggcaccg gccccacctc cggcacctac atgcgcgcca
      541 agctgggcgc gaagcgcgac ggcacgatcg tcgccgccga ggtctacctc gccttcgagg
      601 ccggcgcctt ccccggttcc cccgtcggcg ctggcgtcat gtgcgcggtc gcgccttaca
      661 ccatccccaa cgtccaggtc gacggctacg acatcgtcgt gaaccgcccc aaggtcgccg
      721 cctatcgagg ccccggcgcc ccgcaggccg agttcgccgt cgagtccgtc atcgacgagc
      781 tcgccgagca gctcgagatg gaccccatcg accttcgcct caagaacgcc gccgtcgagg
      841 gcacacgccg cgccgacggc gtcgtcttcg gcccgatcgg caacatcgcc gtcctgcagg
      901 ccatcaagga cagcccgcat taccgctccg agctgcaggg cgagaaccgc ggccgaggcg
      961 tcgccatcgg cttctggttt aacgtcggct tcgaatccgc cgcctacgct tcggtgaacg
     1021 tggacggcac cgtcgccctc agcaccggtt cccccgacat tggcggcacc cgtgccaccc
     1081 acgccatgca gctcgccgag gccctaggca tcccctacga ggacgtcaag ccgcacgtcg
     1141 tcgataccga ctccatcggc tacaccaacg tcaccggcgg cagccgcacc gccttcgccg
     1201 gcggctgggc tgcctacgag gcggccatgg acatcgcccg ccagctcgag gcgcgtgccg
     1261 ccctcatctg ggatgtcgac ccgggcaccg ttcgctacgc ggacggcgtc gtgtccgggc
     1321 ccaacggcaa gagcatgacc ttcaaggaga tcgccgccca gctgccgcac acgggcggca
     1381 tggtgcaggg ccgcgccagc gtgaacccca ccgaggtcgg cccctccttt gccggccaca
     1441 tctgtgacgt cgaggtcgac cccgacaccg gcaaggtcca ggtcctgcgc tacaccgccg
     1501 tgcaggacgt cggccgcgcc gtgcacccct cctacgtcga gggccagatg cagggaggcg
     1561 cgtcgcaggg catcggcatg gccctcaacg aggagt
//
LOCUS       DAORPG010000510         1593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10461_length_1593_cov_2.836801, whole genome shotgun sequence.
ACCESSION   DAORPG010000510 DAORPG010000000
VERSION     DAORPG010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10461_length_1593_cov_2.836801"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLX85_09770"
     CDS             complement(<1..184)
                     /locus_tag="PLX85_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015621295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRC63491.1"
                     /translation="MSRITVSNSITLDGVMQAPARPDEDTRGDFPFGGWALPYNDPVM
                     GETMARRASGGGSLLLG"
     gene            complement(254..787)
                     /gene="folK"
                     /locus_tag="PLX85_09775"
     CDS             complement(254..787)
                     /gene="folK"
                     /locus_tag="PLX85_09775"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRC63492.1"
                     /translation="MTTPGRHEHRGERRVWLALGGNLGDRIAHLRAALDALEASGVAV
                     ETVSSVYETPPWGIEDQPRFVNIVVEARTALDAHALLRLCKRIEADRGRDFSTVRNGP
                     RPIDIDILLIEGEVIDDPDLIVPHRMLQDRAFVLVPLAEVSPGVEHPLLHRTARVLLE
                     DVDPAGIDVLAGPGWWR"
     gene            complement(784..>1593)
                     /gene="folP"
                     /locus_tag="PLX85_09780"
     CDS             complement(784..>1593)
                     /gene="folP"
                     /locus_tag="PLX85_09780"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRC63493.1"
                     /translation="NRAFEWHARTYVMGILNVTPDSFSGDGVLDPSRAAEQAARMVED
                     GADLLDLGAESTRPGAPAVTAEDEWGRLAPVLRAVRAAVDVPLTVDTAKAEVAARAFE
                     AGADALNDVHGLRRDARMATVLATAGCPAILMHNQRGRPTSGDVIADVHAGFEESLRL
                     ARVAGVDERVLILDPGFGFGWQPAQNLELLRRLGELRVLGRPLLLGTSRKSTIGLVLD
                     RPEEERLWGTAATVALAVEAGVDIVRVHDVRAMADVARMADAVVRGWSPPQ"
BASE COUNT          234 a          502 c          633 g          224 t
ORIGIN      
        1 ggccgaggag gagcgagccg ccaccgctcg cgcggcgcgc catcgtctct cccatcacag
       61 ggtcgttgta ggggagggcc cagccgccga aggggaagtc gccacgggtg tcctcgtcgg
      121 gacgggcagg ggcctgcatg acgccatcga gggtgatgct gttgctgacc gtgatgcggc
      181 tcatggcgcg cctccattcc ggctcacctc ggattcccgg ttttcctcgc agggtagtcc
      241 ttgcgagcga gccctatcgc caccagccgg ggccggcgag gacgtcgatg cccgcggggt
      301 cgacgtcttc gaggagcacg cgtgccgtgc ggtggagtag cgggtgttca acaccagggg
      361 agacctcggc cagcgggacg agcacgaaag cgcggtcctg cagcatgcgg tgggggacga
      421 tcaggtcggg atcgtcgatg acctcgccct cgatgagcag gatgtcgatg tcgatggggc
      481 gagggccgtt acggaccgtg ctgaagtcgc ggccgcggtc cgcctcgatg cgcttgcaga
      541 ggcgcagcag cgcgtgggca tcgagagccg tccgcgcctc gacgacgatg tttacaaagc
      601 ggggctgatc ctcgatgccc cagggcgggg tctcgtagac ggacgagacg gtctcgaccg
      661 cgacgccgct ggcctccagg gcgtcgagag cggcgcggag gtgggcgatg cggtccccca
      721 gattgccgcc gagggcgagc cagacgcggc gctcacctcg atgttcgtgt cggcctggag
      781 tggtcattgg ggcgggctcc aaccgcgcac gacggcgtcg gccatgcgtg cgacgtcagc
      841 catcgcgcgg acatcgtgga cgcgcacgat gtcgacaccg gcctcgaccg ccagggcgac
      901 ggtggcggct gtgccccaga ggcgctcctc ctcgggacgg tcgagcacga ggccgatcgt
      961 cgacttgcgc gacgtgccga gaagcagcgg gcggccgagc acgcgcagct cgccgaggcg
     1021 gcggagcagt tcgagattct gagcgggctg ccagccgaag ccgaagccgg ggtcgaggat
     1081 gagcacgcgc tcgtcgactc cggcgacgcg ggcgaggcgg agtgactcct cgaaaccggc
     1141 gtgcacgtcg gcgatgacgt ctcccgaggt cgggcggcct cgctggttgt gcatgaggat
     1201 ggcggggcag cccgccgtgg cgaggacggt ggccatgcgg gcgtcgcggc ggaggccgtg
     1261 gacgtcgttc agggcgtcgg cgccggcctc gaaggcgcgc gcagcgacct cggctttcgc
     1321 ggtgtcgacc gtcaggggga catcgacggc ggcgcggacc gcacggagga cgggggcgag
     1381 acgaccccat tcgtcctcgg cggtcacggc gggagcgccg gggcgcgtcg actccgcgcc
     1441 gaggtcgagg aggtcggctc cgtcctcaac catgcgggcg gcctgttcgg cggcacggga
     1501 cgggtcgagg actccgtcgc cggagaagga gtcgggggtg acgttgagga tccccataac
     1561 gtaggtgcgc gcatgccact cgaaggcgcg gtt
//
LOCUS       DAORPG010000511         1587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10518_length_1587_cov_2.815274, whole genome shotgun sequence.
ACCESSION   DAORPG010000511 DAORPG010000000
VERSION     DAORPG010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10518_length_1587_cov_2.815274"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..598
                     /locus_tag="PLX85_09785"
     CDS             2..598
                     /locus_tag="PLX85_09785"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014789366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRC63494.1"
                     /translation="MLPSRAPASVLGPIVAVLARAGVTPNMLSVTGLLGNGAAGLLVA
                     RGDLIAAGIVMLLASGLDMLDGALARATGKAGPVGALLDSTFDRVSEAAVLFGVLWYA
                     LDRGHDDQAALAFVAIVGSLLVSYVRARVEGLGGRLTDGLFTRAERVVLLALALIFGF
                     LRGALWVLAVLTLVTAAQRLYLGARVLGGPGGTAGGTP"
     gene            595..1404
                     /locus_tag="PLX85_09790"
     CDS             595..1404
                     /locus_tag="PLX85_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63495.1"
                     /translation="MTPSALSREEQAQVMAYLEQQISGSVSLEVWTRKESPLIRTDRE
                     PCVHCEDVVSVARQVAALHPALSVTLYDLDRHERRAQEAGIDRPPTTVLRGRGGRQFR
                     ITGLWAGLLFPPTLDVIAILSAGATPLSDDTRAALASLEQDVTVEVLGAAYDAYSAHM
                     LRLVAAFAVESKRVQASFVNVANYPLLAAARSVEEIPVVFIGNKRFVGVWDGPELAEQ
                     IRRVAGVDDTPVSRGNPMSGPFYTDEEIAALAAERLEEPPRTAGGLYLPGR"
BASE COUNT          191 a          568 c          589 g          239 t
ORIGIN      
        1 gctgctgccc tcacgagcgc cggcgagcgt gctcgggccg atcgtggccg tgctggcgcg
       61 cgccggcgtg acgccaaaca tgctgtcggt gacggggctg ctgggaaacg gcgcggccgg
      121 actgctcgtt gcccggggcg acctcatcgc ggcgggcatc gtgatgctgc tcgcgagcgg
      181 cctcgacatg ctcgatgggg cgctggcacg ggcgacgggc aaggctggcc ccgtcggcgc
      241 gctgctcgac tccacgttcg accgcgtatc ggaggctgcg gtgctgttcg gcgtcctctg
      301 gtacgccctc gaccgcggcc acgacgacca ggcggcgctt gcgttcgtgg ccatcgtcgg
      361 ctccctgctc gtctcctacg tgcgcgcccg cgtcgagggc ctcggcgggc ggctcacgga
      421 cggcctgttc acgcgcgccg agcgggtcgt cctgctggcg ctggcgctga tctttgggtt
      481 cctgcgtggc gcgctctggg tgctcgcggt gctcacgctc gtgaccgcgg cgcagcgcct
      541 gtacctcggc gcgcgggtcc tcggtggacc cggtggcacg gcgggaggga cgccatgacc
      601 ccgagcgcgc tcagccgcga ggagcaggcg caggtcatgg cctacctcga gcagcagatc
      661 tcaggctcgg tctccctcga ggtatggacg cgcaaagagt cgccgctgat ccgcacggac
      721 cgcgaaccat gcgtgcactg cgaggacgtc gtgtccgtgg cgcggcaggt cgccgcgctc
      781 cacccggcgc tctcggtgac gttgtacgac ctcgaccggc acgagcggcg cgcccaggag
      841 gccggaatcg accggccgcc cacgacggtg ctgcgcgggc gcgggggtcg tcagttccgc
      901 atcaccggac tctgggcggg actgctgttc ccgccgacgc tcgacgtgat cgcgatcctc
      961 agcgcgggcg cgacgccgct ctcggacgac acacgcgcgg cgctcgcctc gctggagcag
     1021 gacgtgacgg tcgaagtcct cggggcggcc tacgacgcct actcggcgca catgctgcgg
     1081 ctcgtggcgg ccttcgccgt cgagtcgaag cgggtgcagg cctcgttcgt gaacgtggcg
     1141 aactacccgc tgctcgcggc cgcccgctcc gtcgaggaga tccccgtcgt gttcatcggc
     1201 aacaagcgtt tcgtcggcgt gtgggacggg ccggagctgg ccgagcagat ccgccgcgtc
     1261 gccggtgttg acgacacccc ggtcagccga ggcaacccga tgtcgggtcc gttctacacc
     1321 gacgaggaga tcgcggccct cgccgccgag cgcctcgagg agccgcctcg gaccgcgggc
     1381 gggctctacc ttcccggccg ctgagggcgc ccgcgcaagc tcgacgctac gcccgccgct
     1441 tccgcatcag gtagaaccca ccgccaccga gggcgaccgc gagggcgacc gtgaccgcga
     1501 gcacgaggat ggcggtcgtg gcgttggagc cgccgtccga gtcgttgccg gccgcgctcg
     1561 tgtcgccgct tgagggcgcc ggagtcg
//
LOCUS       DAORPG010000512         1580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10585_length_1580_cov_3.699672, whole genome shotgun sequence.
ACCESSION   DAORPG010000512 DAORPG010000000
VERSION     DAORPG010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10585_length_1580_cov_3.699672"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PLX85_09795"
     CDS             <1..233
                     /locus_tag="PLX85_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018550395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="daunorubicin/doxorubicin resistance ABC
                     transporter ATP-binding protein DrrA"
                     /protein_id="HRC63496.1"
                     /translation="DEAACRLTIPTSDGVPTLLRIGRELDELRVPVVDIGIHRPTLDD
                     VFLVLTGHAAAPTFEDPAAGAAAEERTGARGR"
     gene            223..1053
                     /locus_tag="PLX85_09800"
     CDS             223..1053
                     /locus_tag="PLX85_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019357704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC63497.1"
                     /translation="MAADSSLPHAQPGGIRHAVRDGLIIAVRNLKRVPRIPELAIFAI
                     LQSIMFVLLFAYVFGGAIPLPGGGSYREFLIPGIFAQNVVFAAATTAIGICEDINKGL
                     MDRFRSLPMARSAVLSGRAVFDVIYQSGILVVIMSSGLAVGWRVHSSVPEFFAGVGLL
                     LLFVFAMSWVGIWFGTMVPTVEVANQVAFTVILPVTFLSSVFVPPETLPSVLQPVAEW
                     NPTSALAGALRGFWGNPNPFASSGFPAEHPALLTLIWIAVIVTVFAPLAVLRYRSMSR
                     "
     gene            1131..>1580
                     /locus_tag="PLX85_09805"
     CDS             1131..>1580
                     /locus_tag="PLX85_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63498.1"
                     /translation="MPSELESYHGGLIACGGSTGAIALADRRPFALPGDRAVWGRDRP
                     LVIEHLRLEFAFDLQHREVRGVTTTTFHPRVGGLREAVFDAIELDIEAVTDADGHALP
                     FTASDGKLRIDLGRARPAGRSITTVVRYACRPRRGLYFNQPDEGYPDR"
BASE COUNT          202 a          656 c          443 g          279 t
ORIGIN      
        1 tcgacgaggc cgcctgccgc ctcaccattc ccacctcgga cggcgtcccg acgctcctgc
       61 gtatcggtcg cgagctcgat gagctgcgcg tccccgtcgt cgatatcggc atccaccgcc
      121 cgacgctcga cgacgtgttc ctcgtcctga ccggccacgc cgccgccccc acgttcgagg
      181 accccgcagc cggcgctgct gccgaggaga ggacgggcgc ccgtggccgc tgactcgtcg
      241 ctgcctcacg cacagcccgg cggcatcagg cacgccgtgc gggacggcct catcatcgcc
      301 gtccgcaacc tgaagcgcgt cccgcgcatc ccggagctcg cgatcttcgc gatcctccag
      361 tcgatcatgt tcgtcctgct gtttgcgtac gtcttcggcg gagccatccc cctcccgggc
      421 ggcggctcct accgcgagtt cctcatcccc ggcatcttcg cccagaacgt cgtcttcgcc
      481 gccgccacga ccgccatcgg catctgcgag gacatcaaca agggccttat ggaccgcttc
      541 cgctcgctgc cgatggcccg ctccgccgtc ctcagcggac gcgccgtctt cgacgtcatc
      601 taccagagcg gcatcctcgt cgtgatcatg agctccggcc tcgcggtcgg ctggcgcgtc
      661 cacagcagcg tccccgagtt ctttgccggc gtcggcctcc tcctcctgtt cgtcttcgcg
      721 atgtcctggg tcggcatttg gttcggcacg atggtcccca ccgtggaggt cgccaaccag
      781 gtcgcgttca cggtcatcct ccccgtcacg ttcctgtcga gtgtcttcgt ccccccggaa
      841 acactcccct cggtcctgca acccgtcgcc gagtggaacc ccaccagcgc acttgcgggc
      901 gccctgcgcg gcttctgggg caaccccaac cccttcgcct cctccggctt ccccgccgag
      961 caccccgcat tgctgacgct catctggatc gcggtcatcg tcaccgtctt cgcgccgctc
     1021 gccgtgctgc gctaccgttc catgagccgc tgacaacacc taccctctgc ccgctccccg
     1081 tcgcatgatc gctgtcattc gatgccgaac cgcagccgag ggggcaagcc gtgccgtctg
     1141 aactcgagtc ctatcacgga ggcctgatcg cctgcggcgg atccacgggc gccatcgcgc
     1201 tcgccgaccg ccgtcccttc gcccttcccg gcgaccgcgc cgtctggggc cgcgaccgtc
     1261 ccctggtcat cgaacacctc cgcctcgagt tcgccttcga cctccagcat cgcgaggtcc
     1321 gcggcgtcac cacgacgacc ttccaccccc gcgtcggcgg tctccgcgag gccgtcttcg
     1381 acgccatcga actcgatatc gaggcggtta cggacgccga tggccacgcg ctgcccttca
     1441 ccgccagcga cggcaagctc cgcatcgacc tcggccgcgc ccgccccgcc ggccgctcga
     1501 tcacgaccgt cgtccgctac gcatgccggc cgcgccgcgg cttgtacttc aatcagcccg
     1561 acgagggcta ccccgaccgg
//
LOCUS       DAORPG010000513         1579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10601_length_1579_cov_2.232283, whole genome shotgun sequence.
ACCESSION   DAORPG010000513 DAORPG010000000
VERSION     DAORPG010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10601_length_1579_cov_2.232283"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="PLX85_09810"
     CDS             complement(<1..940)
                     /locus_tag="PLX85_09810"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HRC63499.1"
                     /translation="MALLEISCLGLPEVRHGGASISLPTRKTLALLMYLAIEPGMHSR
                     EALAALFWPEADEERARGALRRVVADLRRSLGRGEGTAEDHIQATRNAIGLERADLRI
                     DLVSPGPAGNGDETQLAGARDPRVAALERYRGELLAGLSLPGADAFEDWLFDRRMRAQ
                     RAITDTCQSLAADRARSGRAAEARSVAARWIEIDRLDERAHRALIEALILLGDRSGAM
                     QAFDSCRALLLRELDVEPEAATVALGMRARSLPAPPSPERPRPGELPFAGRAMEYGQL
                     VEAFQTTREHAPQVVHLAGTSGMGKTRLAREFAEWTR"
     gene            1024..1299
                     /locus_tag="PLX85_09815"
     CDS             1024..1299
                     /locus_tag="PLX85_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63500.1"
                     /translation="MSLTVSFNPVSMTVEQYDEVIRRLEQAGAGKPAGRLHHVCFGFG
                     GHMRVVDIWQSEETFAAFGELLLPILEAVGVDIGQPEVAPVHSVIVG"
     gene            1313..>1579
                     /locus_tag="PLX85_09820"
     CDS             1313..>1579
                     /locus_tag="PLX85_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63501.1"
                     /translation="MDSIALLRSQLEQANQWLEATLGDPARPELHVTPPGDANSAAVS
                     YTHAVISQDLIVAGMLRGAAPLCATSWSNSTGVSETMPMPGPEWV"
BASE COUNT          242 a          586 c          497 g          254 t
ORIGIN      
        1 tcctcgtcca ttcggcaaac tccctcgcga ggcgggtctt tcccattcct gacgtgccag
       61 ccaggtgcac cacctgcggc gcatgctccc gtgtcgtctg gaacgcctcg accagctgac
      121 cgtattccat cgcccgaccc gcgaatggca actcaccggg ccgcgggcgc tctggcgacg
      181 gcggcgctgg caggctccgc gcccgcatgc cgagggccac ggtcgccgcc tccggctcga
      241 catctagctc gcggagcagg agggcacggc acgaatcgaa cgcctgcatc gcccccgagc
      301 ggtcgccgag caagatcagc gcttcgatga gagctcggtg cgcccgctca tccagccggt
      361 cgatctcgat ccaccgcgcc gcgactgacc gcgcttccgc ggctcgtccg ctccgtgctc
      421 ggtcggccgc cagcgactgg cacgtgtcgg tgatcgcccg ctgcgcccgc atccgacggt
      481 caaacaacca gtcctcgaat gcgtccgcgc ccggcagtga caggcccgcg aggagttcac
      541 ctcgataccg ctcgagcgcg gcgacccgag ggtccctggc gcccgcgagc tgcgtctcgt
      601 ccccgttgcc cgccggcccc ggtgacacga ggtcgatccg caggtccgcc cgctcgaggc
      661 cgatcgcgtt gcgggtcgcc tgaatgtggt cttcggcagt cccctcgccc cggccaagtg
      721 agcgccggag gtcggccacg acccgacgga gtgcgccgcg cgctcgctcc tcatccgctt
      781 caggccagaa cagggcggcg agcgcttctc ggctgtgcat ccccggctcg atcgccaggt
      841 acatgaggag cgcaagcgtc tttcgcgtcg gcaatgagat cgacgcgccg ccgtggcgca
      901 cttcggggag ccccaggcac gagatctcga ggagagccat cgttgacgtt cgatctcacg
      961 gtgaaatcac gatcggtgcc tagcatacgg ccgaaatgac acctaccagt gaaaggacgc
     1021 ggcgtgtcac tcaccgtgag cttcaaccca gtctccatga ccgtggagca gtacgacgag
     1081 gtcattcgtc gcctggagca ggccggcgca ggtaagcctg cgggccgcct gcaccacgtc
     1141 tgtttcggct tcggcggtca catgcgtgtc gtcgacatct ggcagtccga ggagaccttc
     1201 gccgccttcg gcgagctgct gctcccgatc ctcgaagccg tcggcgtcga catcggccag
     1261 cccgaggtcg cccccgtcca cagcgtgatc gtcggctagg aggaatcgac tcgtggatag
     1321 catcgccctt ctccgatccc agcttgagca ggcaaaccag tggctcgaag cgacgctcgg
     1381 cgatcccgcc cgtcccgagc tgcacgtcac cccgccgggt gacgcgaact ccgccgccgt
     1441 ctcctacacg cacgccgtga tttcccagga tttgatcgtc gccggcatgc tccggggcgc
     1501 ggccccgcta tgcgcaacgt cctggtcgaa ctcgaccggc gtcagcgaga cgatgcccat
     1561 gcccggcccc gagtgggtc
//
LOCUS       DAORPG010000514         1577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10618_length_1577_cov_2.914586, whole genome shotgun sequence.
ACCESSION   DAORPG010000514 DAORPG010000000
VERSION     DAORPG010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10618_length_1577_cov_2.914586"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..942)
                     /locus_tag="PLX85_09825"
     CDS             complement(55..942)
                     /locus_tag="PLX85_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63502.1"
                     /translation="MRVLKAFLVPGLLIQLLALLLVQHSTSAQFTDQSQSTTNAFAAK
                     TMRAPALTATASGATVSLAWTNPDALSGPNASFIVQRASGDCTAPGVFTTLSGSPFAT
                     TTLATTDAPGANGAYCYALQSALYSWRSSTVTAAATVRVVTVPKLRLLSADPGVCGAV
                     GSLSTMTGASNVVISARSSVIFAAAPAEGLTNVPAGMHVLTIALPGVGSPASVAYTAE
                     VGTCTGTTFTSLGNASDSLVLDKADQTRVVNVRVATGVTLNAGTYLALRVTNNGSANL
                     KLTGNQAESTLEAPAGAGY"
     gene            complement(1008..>1577)
                     /locus_tag="PLX85_09830"
     CDS             complement(1008..>1577)
                     /locus_tag="PLX85_09830"
                     /inference="COORDINATES: protein motif:HMM:NF023806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TasA family protein"
                     /protein_id="HRC63503.1"
                     /translation="NVGWSYTAFTDNAYLTDYKFTDATSTENQFQTGTLNIKLADDNE
                     GAADDVSASISFSNMAPGDSVVKPITVSNPGSLGLRYALTSSSTNADGKDLKGQIVLK
                     VVQVAGTGSCSASAFSGSPAYLYNGALGAAAFGDVTTGAQTGDRAVAASASEVLCLQA
                     SLPIATGNAFQGAATTATFTFTAEQTANN"
BASE COUNT          244 a          449 c          620 g          264 t
ORIGIN      
        1 agggatccga agaagcgggc aagcgtacgc atcgtgcagc ctttctcgtg cgggtcagta
       61 gccggcgccg gcgggcgcct cgagggttga ctcggcctgg ttgccggtga gcttcaggtt
      121 ggcggagccg ttgttcgtaa cacggagcgc cagatatgta ccggcgttca gcgtgacccc
      181 ggtcgcgacc cggacgttca cgacgcgggt ctggtcggcc ttgtcgagga cgaggctgtc
      241 ggaggcattc ccgagggagg tgaacgtcgt gccggtgcac gtccccactt cggccgtata
      301 ggccacgctg gccggggagc caacgccggg cagggcgatc gtcaggacgt gcatgccggc
      361 cgggacgttc gtgaggccct cggcgggtgc tgcggcaaag atgacgctcg aacgtgcgct
      421 gatcacgacg ttgctcgcgc cggtcatggt ggagagcgac ccaacggcac cacacacacc
      481 ggggtcggcg gacagcaggc ggagtttggg aacggtgacg actcggacgg tcgcggcggc
      541 ggtgaccgtc gaggaccgcc acgagtagag cgcgctctgc agcgcgtagc agtaggcgcc
      601 gttggcgccg ggtgcgtcgg tggtcgcgag cgttgtggtg gcgaacggtg agccggagag
      661 ggtcgtgaag acgccgggag cggtgcagtc gccgctcgcg cgctggacga tgaagcttgc
      721 gttggggccg gacagggcgt cggggttggt ccaggcgagg ctgaccgtcg ctccggatgc
      781 ggtggccgtg agtgccggcg cccgcatggt cttcgcagca aaggcgttgg tcgtgctctg
      841 tgactgatca gtgaattgcg cactcgtgga gtgctggacc agcagcagcg cgagcagctg
      901 gatgagcagg ccggggacga gaaaggcctt gagtacgcgc attgcggtag ccccgacggg
      961 cctcggagcc gcattggctc cgaggcccga tgcatgagcg aagcgggtta gttgttcgcg
     1021 gtctgctcag cggtgaaggt gaaggttgcg gtggtcgcgg cgccctggaa ggcgttcccg
     1081 gtggcaatcg ggagcgacgc ctgaaggcag aggacctcgc tcgcgctcgc cgcaacggca
     1141 cggtcgcccg tctgcgcacc ggtggtcacg tcgccgaagg cggcggcgcc gagtgcgccg
     1201 ttgtagaggt acgccggcga gccgctgaag gcggacgcgc tgcacgagcc cgtaccggcg
     1261 acctggacaa ccttgaggac gatctggccc ttgaggtcct tcccgtccgc gttcgtcgag
     1321 gacgaggtca gcgcgtagcg caggccgagc gagccggggt tgctgacggt gatcggcttc
     1381 acgacggagt cgccgggggc catgttcgag aacgagatgc tggcggagac gtcgtcggcc
     1441 gcgccctcgt tgtcgtccgc aagcttgatg ttcagggtgc cagtctggaa ctggttctcg
     1501 gtgctggtgg catcggtgaa cttgtagtcg gtcaaatagg cgttgtcggt gaaggcggtg
     1561 tacgaccagc caacgtt
//
LOCUS       DAORPG010000515         1568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10711_length_1568_cov_4.194316, whole genome shotgun sequence.
ACCESSION   DAORPG010000515 DAORPG010000000
VERSION     DAORPG010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10711_length_1568_cov_4.194316"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLX85_09835"
     CDS             <1..408
                     /locus_tag="PLX85_09835"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63504.1"
                     /translation="IPAGIAAAVAYSPDGDAAVVAEAMQHAIEGVRCVEVTLAARDVQ
                     IGGVVAHEGQPIALLDHVLVATGATLEDALLAGLSAAVTASSEVISVYLGAQAPGGSA
                     DGLQHRLEAAFPDLMVEVVEGGQPYYPYVAGVE"
     gene            418..1287
                     /locus_tag="PLX85_09840"
     CDS             418..1287
                     /locus_tag="PLX85_09840"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRC63505.1"
                     /translation="MALRIVTDSTCDLPPELVAQHRIAVVPLVVLFGDEALQDGVDIS
                     SERFFRRLVRESKLPTTSQPAPAAFRATYERLREEGATEILSIHISGKLSRTLESARQ
                     AAEGLDGVRVVHVDSELATLALGMGVVRAAEAVAAGCGLDEAADIVRSHFRRTHCLFL
                     VDTLEYLRRGGRIGRAQEIVGSLLKVKPILSLEGGEVVPVGRARTKQRAVEEILRRAA
                     EFRPVEQMMAVHATTPEDLKYLVERLHGLAPQARLTVGRITPVIGVHAGPGLLACAVV
                     TAESDSLAPPPEA"
     gene            1287..>1568
                     /locus_tag="PLX85_09845"
     CDS             1287..>1568
                     /locus_tag="PLX85_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63506.1"
                     /translation="MADLERLQTVLEREFRDGCRNDVVEGGLDEFLVRQAQTEPMGSP
                     VLRMVRALPVEGYRSLGADDRRQWIRRAIATVVHERATTAPPKPRRPASG"
BASE COUNT          220 a          538 c          560 g          250 t
ORIGIN      
        1 atccccgcgg gcatcgccgc cgctgtcgct tattcgccgg acggcgacgc cgccgtcgtg
       61 gcggaggcga tgcagcacgc catcgaaggg gtgcgttgcg tcgaggtgac gctcgccgca
      121 cgcgacgtcc aaatcggggg cgtcgtcgcc catgaggggc agccgattgc gctgctcgat
      181 cacgtactgg tcgccaccgg cgcaacgctc gaggacgcgc tgctggcggg actgagtgcg
      241 gcggtcaccg catcttccga agtgatcagc gtctatctcg gggcgcaggc gcccggtgga
      301 agcgctgacg gcctgcagca tcgcctcgag gcggcgttcc cggatctgat ggtcgaggtc
      361 gtagagggcg gccagccgta ctacccgtac gtcgcggggg tcgagtagac tcgcgcgatg
      421 gctctccgga tcgtgaccga ctccacgtgc gatttgccgc cggagttggt tgcccagcat
      481 cggattgctg tggtgccgct ggtcgtgctg tttggagacg aagcgctcca ggatggcgtc
      541 gacatctcct cggaacggtt cttcaggcgg ctggttcgcg aatcgaagct ccccacgaca
      601 tcgcagcccg cgccggccgc cttccgcgcg acctatgagc ggctgcgcga agagggcgcg
      661 accgagatcc tgtcgatcca catctccggc aaactcagcc gcaccctcga gtccgcgcgc
      721 caggccgccg agggcctcga tggcgtgcgc gtcgtgcacg tcgattcgga gctcgccacg
      781 ctggcgcttg gcatgggcgt ggtccgcgcc gccgaggcag tggcggccgg ctgcggcctg
      841 gacgaggcgg ccgacatcgt gcgcagtcac tttcggcgga cgcattgcct gttcctcgtc
      901 gacacgctcg agtacctgag gcgcgggggc cgcatcggtc gcgcgcagga gatcgtcggg
      961 tcgttgctca aggtgaagcc gatactctcg ctcgaggggg gcgaagtcgt gcccgtgggc
     1021 cgcgcccgca ccaagcagag ggcggtcgag gagatcctgc gccgcgctgc cgagtttcgc
     1081 ccggtcgagc agatgatggc cgtccatgcg accacccccg aggacctcaa gtaccttgtc
     1141 gaacggctgc acgggctcgc gccgcaggcg cgcctcacgg tcgggcgcat caccccggtg
     1201 atcggcgtgc acgccggtcc cggcctcctc gcctgcgcgg tcgtgaccgc cgaatccgac
     1261 tccttggcgc cgcctccgga agcctgatgg cggacctcga acgtctgcag acggtccttg
     1321 aacgggagtt ccgggacggc tgccgcaacg atgtcgtcga gggtgggctc gacgagttcc
     1381 tcgtgcggca ggcgcagacc gaaccaatgg gatcgccagt cctgcgcatg gtgcgcgcgc
     1441 tgcccgtcga ggggtatcgc tcgctcggcg ccgacgaccg tcgccaatgg atccgtcgcg
     1501 cgatcgccac ggtcgtccac gagcgcgcga cgacggcgcc gccgaagccg cggcgacctg
     1561 cctccggc
//
LOCUS       DAORPG010000516         1567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10729_length_1567_cov_2.787698, whole genome shotgun sequence.
ACCESSION   DAORPG010000516 DAORPG010000000
VERSION     DAORPG010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10729_length_1567_cov_2.787698"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1567)
                     /gene="lepA"
                     /locus_tag="PLX85_09850"
     CDS             complement(<1..>1567)
                     /gene="lepA"
                     /locus_tag="PLX85_09850"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRC63507.1"
                     /translation="AREMRDQLLDSMDIERERGITIKAQAVRVLWEHHGETYQLNLID
                     TPGHVDFGYEVSRALAACEGAVLVVDATKGIQAQTLANVYQALDLDLTLVPVVNKIDL
                     PAAEPERVAQEVIDVIGFTPDDVIFASAKSGVGIQDILEAVVERVPPPAGDPDAPLRA
                     LVFDSKYDAYKGVIVYVRVVEGTIEAGRGLRLMQQNTRFEPIEVGCFRPAMEVVSSLG
                     PGQVGYVATGLKSVHDSRVGDTITYERHGAEEPLPGYRPAKAMVFAGIYPSDPNQYPE
                     LRDALERLSLNDASLQWEPESSAALGFGFRCGFLGLLHMEIIQERLEREFGLDLVATA
                     PSVEYEVTRTDGREIVIDSPADLPVPNEIGEIREPWMRINVATPSRYLGQVMQLVQDR
                     RGRFEKLEYLDRGGDEGDQRVLAEYLAPLSEILVDFYDQLKSRTQGYASLDYQFHGYV
                     PARVVRLDVLVNGDIVDALSTIVPSENAAAQGRKLISALKELIPRQLFDVAIQAAVGG
                     RIVARETVRAMRKN"
BASE COUNT          251 a          491 c          571 g          254 t
ORIGIN      
        1 cattcttgcg catagcgcgg accgtctcgc gggcgacgat gcggccgccg acggcggcct
       61 ggatggcgac atcgaacagc tgcctcggaa tgagctcttt gagcgcggag atgagcttgc
      121 ggccctgggc agcggcattc tcggacggga cgatcgtcga gagcgcgtcg acgatgtcgc
      181 cgttgacgag gacgtcgagg cgaaccacgc gcgccgggac gtagccgtgg aactgatagt
      241 cgagcgaggc gtacccctgt gtgcgtgact tcagctggtc gtagaagtcg accaggatct
      301 cggacagcgg agccagatac tcggcgagga cgcgctggtc accctcgtcg ccgccgcggt
      361 cgaggtactc gagcttctcg aagcgccctc gacggtcctg taccagctgc atgacctggc
      421 cgaggtagcg cgacggggtg gcgacgttga tgcgcatcca cggctcgcgg atctccccaa
      481 tctcgttggg cacgggcaga tccgccggac tgtcgatcac gatctcgcgg ccatcggtac
      541 gggtgacctc gtactcgacg gagggagcgg tggcgacgag gtcgaggccg aactcgcgct
      601 ccaggcgctc ctggatgatc tccatgtgca ggaggccgag gaacccacag cggaagccga
      661 agccgagcgc ggcggacgac tcgggctccc actgcagcga ggcgtcgttg aggctcagcc
      721 gctcgagggc atcgcgcagc tcggggtact ggttcgggtc ggacgggtag atgccggcga
      781 acaccatcgc tttcgcggga cgataaccgg gcagcggctc ctcggcgccg tggcgctcgt
      841 aggtgatggt gtcgccgaca cggctgtcgt ggacgctctt gagcccggtg gcgacgtagc
      901 ccacctggcc gggaccgagc gaggagacga cctccatggc cggccggaag caaccgacct
      961 cgatcggttc gaagcgggtg ttttgctgca tgagtcggag gccgcggccc gcctcgatgg
     1021 tgccttcgac cacacggacg tagacgatga cgcccttgta ggcgtcgtac ttggagtcga
     1081 agacgagggc gcgcaggggc gcgtcggggt cgcccgccgg gggtgggaca cgctcgacca
     1141 cggcctcgag gatgtcctgg atgccgacgc cgctcttggc ggaggcgaag atcacgtcgt
     1201 ccggcgtgaa gccgatgacg tcgatcacct cctgcgcaac acgctccggt tcggcggcgg
     1261 ggagatcgat cttgttcacg acggggacga gcgtcaggtc gaggtcgagc gcctggtaga
     1321 cgttggcgag cgtctgcgcc tggatcccct tcgtcgcatc gacgacgagg acggcgccct
     1381 cgcaggcggc cagcgcgcgt gacacctcgt agccaaagtc gacgtgaccg ggcgtgtcga
     1441 tcaggttcag ctggtaggtc tcgccgtggt gctcccagag cacgcgcacg gcctgcgcct
     1501 tgatcgtgat gccgcgctcg cgctcgatgt ccatcgagtc gagcagctgg tcgcgcatct
     1561 cgcgcgc
//
LOCUS       DAORPG010000517         1565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10750_length_1565_cov_2.894040, whole genome shotgun sequence.
ACCESSION   DAORPG010000517 DAORPG010000000
VERSION     DAORPG010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10750_length_1565_cov_2.894040"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PLX85_09855"
     CDS             <1..318
                     /locus_tag="PLX85_09855"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRC63508.1"
                     /translation="FIAEQIDSYDFFVGTQTRGFQCGIIYSPDEVFEDPHFVARGFQV
                     PVEHPELGQTFRYPGAPYGFAASPWAISRRPPLVGEHTAEVLAEAGVTPEEFERLRAA
                     GVV"
     gene            complement(333..1421)
                     /locus_tag="PLX85_09860"
     CDS             complement(333..1421)
                     /locus_tag="PLX85_09860"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRC63509.1"
                     /translation="MRVLVMGGTRFNGLALVRELARHGHDVTVLNRGQTPVDLPRGVR
                     RLFGDRTDHEGLKEVLRHEEFDVVQDINGYSVADVQPMIELFRGRVAHYIFASSTVIY
                     APSHVLPMHEWAPVDRGPRQSEYGMNKLLVEDLLFAEHRANGFPATTVAFSMVFGPNN
                     IIPEREQRMFSRLLSGRPVLIPGDGTTLGQVGHVDDEARALRMVMCQPQTFGKRYNLT
                     GKEYYSDEGYVDTFAEVLGVTPRKVHIPAAVMDDIYGDRIALEGSTIASNIETRTAPP
                     RPGSNVGMVQSLIQRLAPNLHHWNRSVLFGIDRLRQDVGWEPEYTFPAMVEQTYEWYR
                     REGLHESRQFDFSWEDRLLERLGAEAGG"
BASE COUNT          232 a          593 c          465 g          275 t
ORIGIN      
        1 ttcatcgccg aacagattga ctcgtacgac ttcttcgtgg gcacccagac ccgcggcttt
       61 cagtgcggga tcatctactc acccgacgag gtctttgagg atccccactt cgtcgcgcgt
      121 ggattccagg tgcccgtcga acatcccgag ctgggccaga cgttccgcta ccccggtgcg
      181 ccctacggct tcgcggcttc gccctgggct atctcgcggc gtccgccgct cgtcggcgag
      241 cacaccgccg aggtcctcgc cgaggcaggc gtcacccccg aggagttcga gcgcctgcgc
      301 gccgccggcg tggtgtagcc ggaccctcgg gcttaacccc cggcctcggc gccaaggcgt
      361 tcgagcagcc ggtcctccca gctgaagtcg aactgccggg actcgtggag tccctcgcgg
      421 cggtaccact cgtacgtctg ctccaccatc gccgggaatg tgtactccgg ctcccagccg
      481 acgtcctgcc gcagccggtc gatgccgaac agcacgctcc gattccagtg gtgcaggttc
      541 ggcgcgagcc gctggatcag gctctgcacc atgccgacgt tcgaccccgg acgcggcggc
      601 gccgtccgtg tctcgatgtt cgacgcgatc gtgctcccct cgagcgcgat ccgatcacca
      661 tagatgtcgt ccatcaccgc cgcgggaatg tgcaccttcc gaggcgtgac gcccagcacc
      721 tcggcaaacg tgtccacgta cccctcgtcg ctgtagtact ccttgccggt caggttgtag
      781 cgcttcccga acgtctgcgg ctggcacatc accatccgca gcgcccgcgc ctcgtcgtcg
      841 acatgcccca cctgcccgag ggtcgtcccg tcaccgggga tgagcacggg ccgcccggac
      901 agcagccgac tgaacatccg ctgctcacgc tccgggatga tgttgttggg cccgaagacc
      961 atcgagaacg cgaccgtcgt cgccggaaag ccgttcgcgc ggtgctccgc gaacagcagg
     1021 tcttccacca gcagtttgtt catcccgtac tcagactgtc gaggtccgcg gtcgaccggc
     1081 gcccactcgt gcatcggcag cacgtgcgac ggcgcgtaga tcacggtcga gctcgcgaag
     1141 atgtagtgcg ccacccggcc gcggaacagc tcgatcatcg gctgcacgtc cgcgaccgag
     1201 tacccgttga tgtcctgcac cacgtcgaac tcctcgtggc gcagcacttc cttcaacccc
     1261 tcgtggtcgg tgcggtcgcc gaacagccgc cgcacccctc gaggcaggtc gaccggcgtc
     1321 tggccccggt tgagcaccgt cacgtcgtgt ccgtgccggg ccagctctcg cacgagcgcc
     1381 agcccgttga acctcgtccc gcccatcacg agcacacgca tcgagagcct cctccgagta
     1441 gcgccgcgac tctaccacag cgttctcagc cggttaccgc gcctgggggc agtgcatact
     1501 gccgccatgc cgctctccga cgacctctgc gctctcccgc tgcaccgtct cgctgcgctg
     1561 cttcg
//
LOCUS       DAORPG010000518         1561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10790_length_1561_cov_3.329349, whole genome shotgun sequence.
ACCESSION   DAORPG010000518 DAORPG010000000
VERSION     DAORPG010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10790_length_1561_cov_3.329349"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLX85_09865"
     CDS             <1..406
                     /locus_tag="PLX85_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HRC63510.1"
                     /translation="SPSPVEARHEVLPAELVAAALRDADVCLVTSRADGMNLVAKEFV
                     AAQRPEAPGVLVLTDGCGAAEELTDALIVPSGDIEALAEAIERALEMPVEERRQRWRS
                     LAATVEAGNVQEWCRGFLATLAGGEAPEVSEP"
     gene            complement(393..1277)
                     /locus_tag="PLX85_09870"
     CDS             complement(393..1277)
                     /locus_tag="PLX85_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRC63511.1"
                     /translation="MPASEPQSEGVIAVDLGGTRLRVAVYDAGGVERHHDTRLTPPGE
                     PEALAQAIVEAKAATSAELRLTEAVVGVPGWVDYRSNRAVALPNLPAWGSIDGAALSS
                     ETGLRVVFGNDADFAALGEHRFGAGRGTTDLVYVTCSTGIGAGVVLGGRLVRSHRSIA
                     EIGHTVIDWRTDDTVEGLGSGTSLARVAGMPAEVVAARAAAGDETAARQFHDAAEALA
                     VGALNMAFLFGPERIVIGGGMAAAGDLLLGPVRERFARERSPLLDLRPEDVVLAEQPQ
                     GAGLAGAFAFWDSEVRAR"
     gene            complement(1292..>1561)
                     /locus_tag="PLX85_09875"
     CDS             complement(1292..>1561)
                     /locus_tag="PLX85_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63512.1"
                     /translation="ALAFARRYEARWLIAAVVIQPSGLSRAAGDPADWADTLALLPTE
                     LAVPMEDAFTGIEVRPLSTAGGAMLPLSAVFRALPVALLEPAPRS"
BASE COUNT          232 a          536 c          591 g          202 t
ORIGIN      
        1 gagtccctcg ccggtcgagg cgcgccacga ggtgctgcca gcggagctgg tggcggcggc
       61 gctgcgcgac gccgacgtgt gcctcgtcac ctcgagggcg gacgggatga acctcgtggc
      121 aaaggagttc gtcgcggcgc agcgccccga ggcgcccggc gtgctcgtgc tgaccgatgg
      181 gtgcggggcg gccgaggagt tgaccgatgc actgatcgtg ccgagcgggg acatcgaggc
      241 gctcgccgag gcgatcgagc gggcactcga gatgccggtc gaagaacgcc gtcagcgctg
      301 gcgctcgctg gcggcgacgg tggaggcggg caacgtccag gagtggtgcc gcggcttcct
      361 ggccaccctc gcgggcgggg aggcccccga ggttagcgag ccctgacctc gctgtcccag
      421 aaggcgaacg caccggcgag ccccgccccc tgtggctgct cagcaagcac gacgtcctcg
      481 ggccgcaggt cgaggagggg cgagcgctcg cgagcgaacc gttcgcggac ggggccgagc
      541 aggaggtcgc cggcggcagc catgccgcct ccgatgacga tgcgttcggg gccgaagaga
      601 aacgccatgt tgagggcgcc gaccgccaga gcctcggcgg cgtcgtggaa ctggcgggcc
      661 gcggtttcgt ccccggctgc ggcgcgggcg gcaacgacct ccgcgggcat ccccgcgacc
      721 cgcgcgagtg acgtgccgga gccgagaccc tcgacggtgt cgtcggtgcg ccagtcgatg
      781 accgtgtggc cgatctccgc gatcgagcgg tgcgagcgca ccagccttcc accgaggacg
      841 acgccggcgc caatacccgt actgcacgtg acgtagacga ggtccgtcgt acctcggccg
      901 gcgccgaagc gatgctctcc gagtgccgcg aagtcggcgt cgttgccgaa cacgactcgc
      961 aggcccgtct ccgaggagag ggctgcgccg tcgatgctcc cccaggcggg cagattggga
     1021 agcgcgacgg cccggttgga gcggtagtcg acccagccag gcacgccgac gaccgcctcg
     1081 gtcagccgaa gttcggccga ggttgctgcc ttcgcctcga cgatcgcctg agccagcgcc
     1141 tccggctcgc ccggcggcgt caggcgggtg tcgtggtggc gctcgacgcc gccggcgtcg
     1201 tagaccgcga cacgcaggcg tgtaccgccg aggtccacgg cgatcacccc ctcagactgc
     1261 ggttcggatg ccggcacggc ttcccttcgc gttacgatct cggtgcaggc tcgagcagcg
     1321 ccacgggcaa cgctcggaac actgcgctca ggggcagcat agcgccgcca gccgtcgaga
     1381 gcggtcgcac ctcgatgcct gtgaacgcgt cttccatcgg gacggcgagc tcagtcggaa
     1441 ggagggcgag cgtatcggcc cagtccgcgg gatcgcccgc ggcacgcgag agtccggacg
     1501 gctggatgac gactgccgcg atgagccagc gcgcctcgta acgacgcgcg aaggcgagtg
     1561 c
//
LOCUS       DAORPG010000519         1560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10800_length_1560_cov_3.462458, whole genome shotgun sequence.
ACCESSION   DAORPG010000519 DAORPG010000000
VERSION     DAORPG010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10800_length_1560_cov_3.462458"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1108)
                     /locus_tag="PLX85_09880"
     CDS             complement(8..1108)
                     /locus_tag="PLX85_09880"
                     /inference="COORDINATES: protein motif:HMM:NF021895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRC63513.1"
                     /translation="MDVIACHQAGIRNVVASMGTSITDRQMTLLQRYTHNVVLMLDAD
                     NAGSEATLRGVEVAAGAAEHGLAPTVDWRGLVSYQDVLKADIRVIALPDGEDPDSLVR
                     SDPDRVRALLGAAKPVADHLFDAVSATTDFADPRARSRALEALAPTVAAVTDPVVRAH
                     YVQKMARMAQVDERTVLALLNRGRARPAAVPSPRDVAQAAARGASTTPDGETQLLQLL
                     VLRSEARPAGRILDPDTFEDTTNRLLFEAWREFRELDEHLDELDDDVRARLDAVRGSA
                     NGIWDPDAIDAKFVEPRVSEIAERLRLRRAQARLLPAAVAVATDLAASRRSGSSDDGL
                     VAEFDELHQRQRALSRRARGEAAVESEGAPAV"
     gene            1088..>1560
                     /locus_tag="PLX85_09885"
     CDS             1088..>1560
                     /locus_tag="PLX85_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63514.1"
                     /translation="MAGDHVHVALNDDHAVGAAGFLASAVEAVQRAALVEEGGLRGVE
                     VLRVLVGGEGARAEADEPASTVSSREDQTVAKAVVEPTITVDGEAGIAQVGVGEATRA
                     EVLDEAVPFVGGIADLPGLDGGGVETALLGVGEGRGARRAVDRRLRDLHHQHRGAR"
BASE COUNT          222 a          494 c          608 g          236 t
ORIGIN      
        1 ttgatgttca gacggcgggc gcgccttcgc tctccaccgc cgcttctcca cgtgcccgtc
       61 gcgagagggc gcgctggcgt tgatggagct catcgaactc cgcgacgagg ccgtcgtcgg
      121 aactcccgct gcgcctcgat gctgcgaggt cggttgccac cgcgacggcg gcaggcagca
      181 acctcgcctg cgcccggcgc agccggaggc gctcggcgat ctcggagacg cgcggctcga
      241 cgaacttcgc gtcgatcgcg tcgggatccc agatgccgtt cgccgagccg cggacggcgt
      301 cgagacgagc gcggacgtca tcgtcgagct catcgagatg ctcatccagc tcgcggaact
      361 cgcgccatgc ctcgaagagg aggcgattcg tcgtgtcctc gaaggtgtcc gggtcgagga
      421 tgcgcccggc cgggcgtgcc tcggaacgga ggacaaggag ctggaggagt tgggtctcac
      481 catcgggtgt cgtgctggca ccacgagcgg cggcctgtgc cacgtcgcgc ggggagggca
      541 cggcggccgg acgggcacga cctcggttga ggagggcgag cacggtgcgc tcgtcgacct
      601 gcgccatgcg cgccatcttc tggacataat gggctcggac gactggatcg gtgacggcgg
      661 caacggttgg cgcaagcgct tcgagggcgc gagagcgcgc tcgggggtcg gcgaagtccg
      721 tggtcgcgct cacggcgtcg aagaggtggt cggcgaccgg cttcgcggcg ccgaggagtg
      781 cccggacgcg gtcggggtcg gagcgtacga gggagtcggg gtcctcgccg tcgggtagcg
      841 cgatcacgcg gatgtccgct ttcagcacgt cctggtagga gaccaggccg cgccagtcga
      901 cggtgggcgc gaggccgtgc tcggctgcgc cggctgccac ctcgacaccg cgcagggtcg
      961 cctcggagcc cgcgttgtcg gcgtcgagca tcaggacgac gttatgcgtg tagcgctgca
     1021 gcagcgtcat ctggcggtcg gtgatggagg tgcccatgga ggcgacgaca ttgcggatgc
     1081 ccgcctggtg gcaggcgatc acgtccatgt agccctcaac gacgaccatg cggtcggcgc
     1141 ggcgggcttc ctcgccagcg cggtcgaggc cgtacagcgt gccgctcttg tcgaagaggg
     1201 gggtctgcgg ggtgttgagg tacttcgggt cctggtcggc ggcgagggcg cgcgcgccga
     1261 agccgatgag ccggcctcga ccgtctcgag tcgggaagat cagacggtcg cgaaagcggt
     1321 cgtagagccc acgatcaccg tcgatggcga ggccggcatc gcgcaggtcg gcgtcggtga
     1381 agccacgcgc gcggaggtgc tcgacgaggc cgtgccattc gtcgggggca tagccgatct
     1441 gccaggcctg gacggtggcg gcgtcgagac cgcgcttctc ggcgtaggtg agggccgcgg
     1501 cgccagacgg gccgtggatc gccgcctccg ggacctacac caccaacatc ggggtgctcg
//
LOCUS       DAORPG010000520         1556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10858_length_1556_cov_2.435043, whole genome shotgun sequence.
ACCESSION   DAORPG010000520 DAORPG010000000
VERSION     DAORPG010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10858_length_1556_cov_2.435043"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..485)
                     /locus_tag="PLX85_09890"
     CDS             complement(81..485)
                     /locus_tag="PLX85_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63515.1"
                     /translation="MTRAASILLHAVLGVAAIAAGQAFARRPSGRDLGMDPRWLEGSP
                     FPDFRIPGLFLLLVIAPANLLSALLLLRRHRGGPPLSLATGLLLVVWLAIQTAILGFR
                     DRSQAIWWLLFPLVAVLGAAQLRARRGEADAR"
     gene            530..>1556
                     /locus_tag="PLX85_09895"
     CDS             530..>1556
                     /locus_tag="PLX85_09895"
                     /inference="COORDINATES: protein motif:HMM:NF018180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HRC63516.1"
                     /translation="MRAEPFVIDVPDEEIAELRERLERTRWPHQAAGNEGWEWGTNLE
                     FMQHLVGYWREEYDWRAVERRLNRFSHYRAAITGENGEEHQIHFVLERGSGSRPRPII
                     LTHGWPSTFVEYLDAAERLAHPERFGGDAEDGFDVVVPSCPGFGFSSQPRLPIAPRAM
                     AYLWDQLMRELLGYPRYIAHGGDLGAFITAQLGLHYASSLYGIHLTLLPLVFRLNDGV
                     QAPVTAEEQAYLDGRERRSRGGMGYSAIQSTKPMALAYGLTDSPAGLAAWIADKYFAW
                     SDFERSGSSAAFEQRVPLEQLVTMASIYWFTGTIASANNVYKAGQIERSAGLGPGERV
                     RVPTGYAD"
BASE COUNT          241 a          436 c          673 g          206 t
ORIGIN      
        1 gacgcgaggg cggacgaagc ggaggtaggc gacgaaagcg gcgccgagga ggagcgcggt
       61 cttgagcagc gcggcgggtt tcatcgagcg tccgcttcgc ctcggcgggc gcggagctgg
      121 gctgcgccga ggacggcgac gagggggaac aggagccacc agatcgcctg gctcctgtcg
      181 cggaagccga ggatggcggt ctggatggcg agccagacga cgagcaggag gccggtggcg
      241 aggctgaggg gcgggccgcc tcgatggcga cgcaggagga ggagcgcgct caggaggttg
      301 gcgggggcga tgacgaggag caggaataga ccggggatgc ggaagtcggg gaacggtgag
      361 ccctcgagcc agcgtgggtc catgccgagg tcgcggccgc tggggcgcct cgcgaaggcc
      421 tggccggcgg cgatggcggc gacgccgagg acggcgtgga ggaggatcga ggcggcgcgg
      481 gtcatggggc tatgatgcca gcccgcgcga gcgggacggg aggtggggca tgcgagcgga
      541 gccgttcgtc atcgatgtgc cggacgagga gatcgcggag ttgcgggagc gcctcgaacg
      601 gacgcgctgg ccgcaccagg cagccggcaa cgagggctgg gagtggggga cgaacctcga
      661 gttcatgcag cacctcgtgg ggtactggcg cgaggagtat gactggcgcg cggtggagcg
      721 tcggctgaac cgcttcagcc attaccgggc ggcgatcacg ggtgagaacg gggaggaaca
      781 ccagatccac ttcgtcctcg agcgggggtc ggggagccgg ccgcggccga tcatcctgac
      841 gcacgggtgg ccctcgacgt tcgtggagta cctcgatgcg gcggagcggc tggcgcaccc
      901 ggagcggttc gggggcgacg ccgaggacgg gttcgacgtc gtggtgccgt cgtgtccggg
      961 gttcgggttc tcgtcgcagc cgcggctgcc gatcgcgcct cgcgcgatgg cgtacctgtg
     1021 ggaccagttg atgcgggagc tgcttggcta cccgcggtac atcgcgcacg ggggcgacct
     1081 cggagcgttc atcacggcgc agctggggct gcactacgcg tcgagcctgt acgggatcca
     1141 cctgacgttg ctgccgctgg tgtttcggct gaacgacggg gtgcaagcgc cggtgacggc
     1201 ggaggagcag gcgtacctcg acgggcggga gcggcgcagc cggggcggga tggggtattc
     1261 ggcgatccag agcacgaagc cgatggcgct ggcgtacggg ctgacggact cgcccgcggg
     1321 cctcgcggcg tggatcgcgg acaagtactt cgcgtggtcg gacttcgagc ggtcggggtc
     1381 gagcgcggcg ttcgaacagc gggtgccgct cgagcagctc gtgacgatgg cctcaatcta
     1441 ctggttcacg gggacgatcg cgtcggcgaa caacgtgtac aaggcggggc agatcgagcg
     1501 gagcgccggc ctggggccgg gcgagcgggt gcgcgtgccg acggggtacg cggact
//
LOCUS       DAORPG010000521         1554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10881_length_1554_cov_2.693796, whole genome shotgun sequence.
ACCESSION   DAORPG010000521 DAORPG010000000
VERSION     DAORPG010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10881_length_1554_cov_2.693796"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLX85_09900"
     CDS             <1..254
                     /locus_tag="PLX85_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63517.1"
                     /translation="AFAVEGIPPSEVSAQLWLAGRVVCRAVAETNCVRLTLHVYNNES
                     DIDVVAGVIEDAIKTGVSEAARELAAIPFGQPAPTSLTN"
     gene            complement(251..1267)
                     /locus_tag="PLX85_09905"
     CDS             complement(251..1267)
                     /locus_tag="PLX85_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifH"
                     /protein_id="HRC63518.1"
                     /translation="MGWQDHLNGDPLPWLLEHSNPAVRAATLQRLLDAALDDPEVVRA
                     REEAMQVDPIASILAAQQPDGWWEKPGPGYSPKYRSTVWQLIFLDHLGADPSHPQVRR
                     ACEYVMRWAPTPSGGFASSGVGQERNPPPARVIHCLNGNLLHALIAFGHLEAPAVQAG
                     VHWAARSILGTDEVTYYRSGTSGPGFACVANDGLPCAWGAIKELGALALIPQKQRSTR
                     VGQAIEAGVEFLLSRDPAAADYPMGYGNTKPAASWFRFGFPSAYVADVLQNLEVLAAL
                     GHARDARLDHAYELVLSGQDDDGRWRNRYAYNGRTTVDIERQGEPSKWVTLRACAVLK
                     ARYG"
     gene            1307..>1554
                     /locus_tag="PLX85_09910"
     CDS             1307..>1554
                     /locus_tag="PLX85_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63519.1"
                     /translation="MGDNVGMTIPPRPPGPIAALRRIAYLLEYAGESSYRASAFRRAA
                     DAVAELAYEDLRARAEGLDITCKVGFLERPERVVLLIIG"
BASE COUNT          215 a          555 c          528 g          256 t
ORIGIN      
        1 tcgccttcgc cgtcgagggc atcccgccgt ccgaggtctc tgcgcagctc tggctggcgg
       61 gccgcgttgt ctgccgcgcc gtcgccgaga cgaactgcgt ccgtctgaca ctccacgtct
      121 acaacaacga gtcggacatc gatgtcgttg cgggcgtcat cgaggacgct atcaagaccg
      181 gcgtctccga ggcggctcgt gagctggcgg ccatcccgtt cggccagccc gcgccaacct
      241 cgctgacgaa ctagccgtaa cgtgctttca gcacggcgca cgcgcggagc gtgacccact
      301 tcgagggctc accctgccgc tcgatgtcga cggtcgtccg cccgttgtag gcgtaccggt
      361 tccgccagcg cccgtcgtcg tcctgcccgg acagcactag ctcgtacgcg tggtcgaggc
      421 gcgcgtcgcg ggcgtgccca agcgcggcca gcacctcgag gttctgcagc acgtcggcga
      481 cgtacgccga aggaaagccg aatctgaacc aggacgcggc cggcttcgtg ttgccgtaac
      541 ccatcgggta gtcggcggcc gccgggtcgc gcgagagcag gaattccact cccgcctcga
      601 tggcctggcc cacccgggtt gagcgttgtt tctgggggat gagcgccagg gctcccagtt
      661 ctttgatcgc gccccacgcg cacggcagcc cgtcattcgc gacgcacgcg aagccagggc
      721 cgctcgttcc cgaccggtag tacgtcacct cgtccgtccc gaggatcgac cgcgccgccc
      781 agtgcacgcc ggcctgcacg gcgggggcct cgaggtgccc gaacgcgatc aacgcgtgca
      841 gcaggttgcc gttcaggcag tggatgacgc gtgcgggcgg cggattgcgc tcctgtccca
      901 cgccgctcga ggcgaaacca ccgctcggcg tcggcgccca ccgcatgacg tactcgcatg
      961 cgcgtcgcac ctgtggatgc gatgggtccg cgccgaggtg gtcgaggaag atgagctgcc
     1021 acaccgtgct tcggtacttc gggctgtatc ccggccccgg cttctcccac catccgtccg
     1081 gctgttgcgc cgcaaggatc gaggcgatcg ggtcgacctg catcgcctcc tcgcgcgccc
     1141 gcacgacttc gggatcgtcg agcgcggcat cgaggagccg ctgcagcgtg gcggcacgca
     1201 ccgccgggtt cgagtgctcg agcagccacg ggagcgggtc gccgttgagg tggtcttgcc
     1261 agcccatccc cccgagggta gcgactccgc gttcgatgtg aggagcgtgg gcgacaatgt
     1321 tggcatgacc atcccgccga ggccgcctgg ccccatcgcc gcgctgcgcc gcattgccta
     1381 cctcctcgag tacgcgggcg agtcctcgta ccgcgcgtcc gcgttccggc gcgccgctga
     1441 tgccgtcgcg gaactcgcgt acgaggatct gcgcgcgcgc gccgaggggc tcgacattac
     1501 gtgcaaggtc gggttcctgg agcggcccga gcgcgtcgtg ctgctgatca tcgg
//
LOCUS       DAORPG010000522         1548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_10941_length_1548_cov_3.441393, whole genome shotgun sequence.
ACCESSION   DAORPG010000522 DAORPG010000000
VERSION     DAORPG010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10941_length_1548_cov_3.441393"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="PLX85_09915"
     CDS             complement(<1..873)
                     /locus_tag="PLX85_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRC63520.1"
                     /translation="MNGGASEDFGARLSFDRSEPGRRAVDVPRWDGDLAAFPDARLLR
                     EGVRLPELSQPGLVRYYTALSQRNYGVDSGTYPLGSCTMKYNPKVNEAIAALPGFADA
                     HPLAPAEDVQGTLEVLHGLQRGLAEITGMAAVSLAPAAGAQGELAGILMVKRALEQRG
                     ELARRRRVLVPDSAHGTNPATAAMAGFEVTQIPSQADGSMDDDVLARELDDTVAALMV
                     TVPNTLGLWEHGIEDIARQAHEAGALMYGDGANLNAVLGRVRFGDLGFDVVHLNLHKS
                     FSTPHGGGGPGAGPV"
     gene            complement(870..>1548)
                     /locus_tag="PLX85_09920"
     CDS             complement(870..>1548)
                     /locus_tag="PLX85_09920"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF014408.2"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HRC63521.1"
                     /translation="VNADPFALGMLRAPGDAGADVVTGEGRDLAGGLQFGGPSLGLFA
                     ARSEYLRQLPGRIVGRTRELHGPRDGGGHEQAPRTGYVLTLQAREQFIRRERATSNVS
                     TAQALIALAFTIALQALGPRGLREAAELCYQRAHEAARRIEALPAFEVPSSGPWFQEF
                     LVRGPVRAPELHRRLAERGIAAGLDVSQRDEPEARDALLFCVTEATPPEHVDALIDAL
                     REIGDTL"
BASE COUNT          219 a          566 c          546 g          217 t
ORIGIN      
        1 gacagggcct gcacccgggc cgccgccgcc gtgcggtgtg ctgaacgact tgtgcaggtt
       61 caggtgcacg acgtcgaaac cgaggtcgcc gaagcgtacc cggccgagga ccgcattcag
      121 gttcgcgccg tcgccataca tcagcgcgcc ggcctcgtgc gcctgccgcg cgatgtcctc
      181 gatgccgtgc tcccacagcc cgagggtgtt cgggacggtc accatcagcg cggcgacggt
      241 gtcgtcgagt tcgcgggcga gcacgtcgtc atccatcgag ccgtctgcct ggctgggaat
      301 ctgggtcacc tcgaagccgg ccattgcggc ggtcgccggg ttggtgccgt gagcagagtc
      361 gggcacgagc acccgccggc ggcgagccag ctcgccgcgc tgctcgagcg cgcgcttgac
      421 catcaggatg ccggccagct cgccctgcgc tcccgcagcg ggcgcgaggc tcaccgcggc
      481 catcccggtg atctcagcga gcccgcgctg gagcccgtgc agcacctcga gggtgccctg
      541 cacgtcctcg gcgggcgcga gcggatgcgc atctgcgaag ccgggcagcg cggcgatcgc
      601 ttcattgacc ttggggttgt acttcatcgt gcacgagccg agcgggtagg tgccggagtc
      661 gacgccgtag ttccgctgcg acagcgccgt gtagtaccgc acgaggccgg gctggctcag
      721 ctcgggcagc cgcacgccct cgcgcaggag ccgcgcgtcg ggaaacgccg cgagatcacc
      781 atcccaccgg ggcacgtcga cagcccgccg ccctggctcc gagcggtcga atgacaaccg
      841 cgccccgaag tcctcgctgg cgcccccgtt cacagggtgt ctccgatctc gcgcagcgca
      901 tcgatcagcg catcgacgtg ctcgggcggt gtggcctccg tcacgcagaa cagcaaggcg
      961 tcccgcgcct cgggctcatc gcgctgagag acatcgaggc ctgcggcgat gccacgctcg
     1021 gcgagacggc ggtgcaactc gggcgcgcgc acgggccccc gcacgaggaa ctcctggaac
     1081 cacgggccac tcgaaggcac ctcgaaggcc ggcagcgcct cgatacgacg cgcggcctcg
     1141 tgcgcgcgct ggtagcacag ttcggctgcc tcgcgcagcc cgcgcggtcc gagcgcttgc
     1201 aacgcgatcg tgaacgcgag ggcgatcagt gcctgtgccg ttgagacgtt cgaggtggcc
     1261 cgctcgcggc ggatgaactg ctcgcgtgcc tgcagcgtga gcacgtagcc ggtgcggggc
     1321 gcctgttcgt ggccgccccc gtctcgaggg ccgtgcaact cgcgtgtccg ccccacgatt
     1381 cgtcccggca gctgacgcag gtactcactc ctcgcggcaa agaggccgag cgaggggccg
     1441 ccgaactgca gcccgcccgc gaggtcgcgc ccttcgcccg tcacgacatc cgcgccggcg
     1501 tccccgggcg cgcgcagcat cccgagggcg aacgggtccg cgttcacg
//
LOCUS       DAORPG010000523         1543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_11006_length_1543_cov_3.553763, whole genome shotgun sequence.
ACCESSION   DAORPG010000523 DAORPG010000000
VERSION     DAORPG010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11006_length_1543_cov_3.553763"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PLX85_09925"
     CDS             complement(<1..790)
                     /locus_tag="PLX85_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63522.1"
                     /translation="MAMMQNQRTITITAMSRPHLFRGLLESLVTNDLDGWSVIVRVEP
                     GGRAEEFAPIAAELLAGVDYDLRINSERRGITLNPFLTLDETFAVGSELNLYLEEDLL
                     VSPDVLALAEWYERQHRDSWLCLSLLAGPCGSAGLLSNADYPSLLFEARTFNSIGFVL
                     RREEWFKHVRPVWLSREAVDGGIHSNWRLNWGWDWSVYGLVASDPTLRTVQPVLARAT
                     HNGREGGVYALPAFHDAAFDGLPINRASVDAYRLVAVADLPPDVR"
     gene            908..1363
                     /locus_tag="PLX85_09930"
     CDS             908..1363
                     /locus_tag="PLX85_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRC63523.1"
                     /translation="MFRKVLVTVDRSPFAEAALDRVPDINAAAVVILHVVEPVASILA
                     REVPAFDVPPDIATSISQAEREAAAAYLSEAATKLRGAGVGQVSTLMREGRPGPEIVA
                     AAREEGCDLVVMSTHGRSGIRRALLGSVANHVTTHVEDGAILLVRPPQD"
BASE COUNT          270 a          512 c          529 g          232 t
ORIGIN      
        1 cgcgcacgtc gggggggaga tcggccacgg cgaccagccg gtaggcgtcg accgatgctc
       61 ggttgatagg cagcccgtcg aaggcggcgt cgtggaacgc cggaagggcg tatacgccgc
      121 cttcgcgccc gttgtgcgtg gcccgagcga gcaccggctg caccgtccgc agcgtgggat
      181 cgctggcgac cagcccgtag accgaccagt cccagcccca gttgagtcgc cagtttgagt
      241 ggatgccgcc gtcgacggcc tcgcgagaca gccatacggg acgcacgtgc ttgaaccact
      301 cctcgcggcg cagcacgaag ccgatcgaat tgaacgtgcg tgcctcgaac aggagcgacg
      361 gatagtccgc attcgagagc aagcctgccg agccgcatgg acctgcgagc agactgaggc
      421 agagccacga gtcgcgatgc tgccgctcat accattctgc gagcgccagg acgtccggcg
      481 acacgagtag atcttcctcg aggtacaggt tcagctcgga accgaccgcg aacgtctcgt
      541 cgagcgtgag gaacgggttc agggtgatcc cgcgacgctc cgagttgatg cggaggtcgt
      601 agtcgacgcc cgcgagcagc tcggcagcga tgggggcgaa ctcctcggct cgtccgccgg
      661 gctcgacgcg gacgatgacg ctccacccat cgaggtcatt cgtcaccagt gattccagca
      721 gccctcgaaa caggtggggc cggctcatcg cggtgatcgt gatcgtccgc tggttctgca
      781 tcatcgccat cctgatcgag ccaagccggg gcgcgcaagc cgtaatgagg gactacgccg
      841 tgctttgggc cagcggcgac cctagaatcg ggagcaggtg tagaccgcga acgacaggag
      901 gattccgatg tttcgcaagg tactcgtgac ggtggaccgt tcacccttcg ccgaggcggc
      961 cctcgaccgc gtccctgaca tcaacgccgc ggccgtcgtc atcctgcacg tcgtcgagcc
     1021 ggtggcgagc atcctcgcgc gcgaggtgcc cgccttcgat gttccgcccg acatcgcgac
     1081 gagcatcagc caggccgaac gagaagccgc ggcggcatac ctctcggaag ccgcgacgaa
     1141 actgcgcggc gctggggtcg gccaggtcag cacgctgatg cgcgaagggc gtcccgggcc
     1201 ggagatcgtc gccgccgcac gcgaggaagg ctgcgacctg gtcgtgatga gtacgcacgg
     1261 ccgctccggc atccgccgag cgctgctggg ctcggtggcg aaccacgtca cgacgcacgt
     1321 cgaagacggc gcgatcctcc tcgtgcggcc gccgcaggac tgacccgtcc gtcccacgcg
     1381 gccaatacga gaagcggcct ccctttcggg aggccgcttc gattcgcgcc gagagcggga
     1441 cctaaccgcg aggcaggccg agaccgcgcg tggcgatgat gttgcgctga atctcgttgg
     1501 agccgcccgc gatggtgatg accacggtgc tcacgtactg ctg
//
LOCUS       DAORPG010000524         1540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_11032_length_1540_cov_3.810101, whole genome shotgun sequence.
ACCESSION   DAORPG010000524 DAORPG010000000
VERSION     DAORPG010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11032_length_1540_cov_3.810101"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..>1540
                     /locus_tag="PLX85_09935"
     CDS             281..>1540
                     /locus_tag="PLX85_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63524.1"
                     /translation="MNRAVGVVVAAAVAALAVALGTSAPRVVEAASVAVTSSADSGPG
                     SFRQAVLDANGNAGINLIVLKNGQPITLNSPVEYTGAQALTIQGDSFLVNRVIQGVHG
                     PDECYLLRSSGGADLTLTRVTMQDSDCGGVEVTGGSGFGEIVVTVTRSTFHDLGLSGI
                     RLADAFTDASFTFSAALSHFDDNGLNGVEVFELDAGQARVSVNGSSFDRNAFEGLHIE
                     ETGEGDVTLRAGTSSFSGNGHGIVVQEADTGNLDVGLTSVQANGSRSRSGVELTEIDS
                     GNLMLAGSVVVVNGGFGSGLAAVELGEGDLVGRLTGVQVRDNSQTGLALLELDSGKLD
                     AAVSWSIADGNEVGFSFGEGGEGDLSVLVFGSTASHNELGFALGETDDGAFNARMLVT
                     TSLLNDAGVEAEAIGDPGTLDRTGSPLL"
BASE COUNT          249 a          405 c          620 g          266 t
ORIGIN      
        1 gtcgtgctgc aggaagaggg cgcgggcgac gtacggttcg gtgcaggggg atttgtcccg
       61 cagctgagcg atggcgaccg ggcgttcatc ggaccgttcg aaccggttgc gccgacgggc
      121 gacggcaatg agccttcggc gctgctgatc ccggtgccgt agcttcacga gggccggacc
      181 gagcctctat tgtttgacag gggccccgca ctcccggtat caagggccgg ctcagtcgtt
      241 gcccaggggg tgggcaagca gaggcggggg agagagtcag atgaaccgtg cggtgggtgt
      301 ggtagtcgcg gcggcagtcg cggcgctggc ggtggcgctc gggacgagcg caccgcgggt
      361 ggtggaggcc gcctcggtgg cggtgacgag ctcggcagac tcggggccgg ggtcgtttcg
      421 gcaggcagtc ctggacgcga acgggaacgc cggcatcaat ctcatcgtgc tgaagaacgg
      481 tcagccgatc acgctcaatt caccagtcga gtacacgggc gcgcaggcgc tgacgatcca
      541 gggtgacagt ttccttgtga accgggtgat ccagggcgtc cacggcccgg acgagtgtta
      601 cctgttacgg agcagtggcg gtgcggacct gacattgact cgggtcacga tgcaggactc
      661 ggactgtggg ggcgtggagg tgacgggggg gagcggattc ggggagatcg tggtcacggt
      721 cacccgttcg acgttccatg acctcgggtt gagcggcatc cggctggcgg acgcgttcac
      781 cgatgcgtcg ttcaccttca gcgcggcgct gtcgcacttc gatgacaatg ggctgaatgg
      841 ggttgaggtg ttcgagctgg atgccgggca ggcgcgcgtt tccgtgaacg ggtcgagctt
      901 cgaccggaac gcgtttgaag gcctgcatat cgaggagacg ggcgagggtg atgtgacgct
      961 gcgcgccggg acgagcagct tcagcgggaa tgggcacgga attgtcgtgc aggaggcgga
     1021 cacgggtaac ctcgatgtcg ggctgacgag cgtgcaggcg aacgggtcgc ggtcccggag
     1081 tggagtcgag ctgactgaga tcgattcggg aaacctcatg ctcgcggggt ccgttgtcgt
     1141 ggtgaatggt ggcttcggga gcggattggc ggcggtggag ctgggcgagg gggacctcgt
     1201 cgggcggctc accggggtgc aagtacgaga caacagccag acaggcctgg cgctgctgga
     1261 gctcgacagc gggaagctcg atgcggcggt gtcgtggtcg attgccgacg gcaacgaggt
     1321 tggattctcc ttcggagagg gaggagaggg cgacctgtcg gtcctggtgt tcggctccac
     1381 ggccagccac aacgaacttg gctttgcgct tggtgagacc gacgacgggg cgttcaacgc
     1441 gaggatgctc gtgacgacgt cgttgctgaa tgacgcgggg gtggaggccg aggcgatcgg
     1501 cgacccgggc acgctggacc gtacggggtc gccgctgctc
//
LOCUS       DAORPG010000525         1540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_11037_length_1540_cov_2.902357, whole genome shotgun sequence.
ACCESSION   DAORPG010000525 DAORPG010000000
VERSION     DAORPG010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11037_length_1540_cov_2.902357"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..689
                     /locus_tag="PLX85_09940"
     CDS             141..689
                     /locus_tag="PLX85_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63525.1"
                     /translation="MTAYRGLRRAPAKPRVASAAPLLLAALLAAAVACGGGSEPTSGV
                     GGSKSGATPIFSGATTATIEATPPTNTPPTTTPPVTTPVATGAPSGTVTIAGTRYAFT
                     VRACTLINGQHYLVGDGEATIALSTVLLIQTPARGSFSVDNPTVSVSGNTLTASGTGI
                     NIALPRERPDVTLTATCTGLAN"
     gene            724..1515
                     /gene="ppk2"
                     /locus_tag="PLX85_09945"
     CDS             724..1515
                     /gene="ppk2"
                     /locus_tag="PLX85_09945"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HRC63526.1"
                     /translation="MPDPQPPPYERISTKQYERLLHDLQVELVKLQRDFIRDGERTLV
                     ILEGRDAAGKDGTIKRITEHLSPRETRVVALGKPSDRDQTTWYFQRYAPHLPAASELV
                     LFNRSWYNRAGVERVMHFCTADQYEEFMTSVLEFEHMLIRSGIRLFKYYLDISKAEQK
                     ERLEDRRKSPLTQWKISSLDEKALDLWDDYSEARNDMLLRTRDPIAPWIVVRANHKKT
                     ARVQLIQDLLSRLDYEDKDTSLLKPDPSVVFEFDPVHLENGMLEP"
BASE COUNT          294 a          647 c          376 g          223 t
ORIGIN      
        1 tttccatggc gggagggtgg gccttccgaa aaacaacagc cggccgcgca gcggccgtcc
       61 tcctcgcagc gcgctcccag ctccaccctg ccccataatc aaccggctct ctcctgttac
      121 cgccggaaag gcagctcgcc atgaccgcct accgaggcct ccggcgcgcc cccgcgaaac
      181 cccgcgtcgc cagcgccgcc ccgctgctcc tcgctgcact cctcgccgca gccgtcgcct
      241 gtggcggcgg ctccgaaccc acctccgggg ttggcgggtc gaagagcggc gccaccccca
      301 tcttcagcgg cgccaccacc gccaccatcg aggccacccc acccacgaac acacccccga
      361 cgaccacgcc gcccgtcacg acacccgtcg ccaccggcgc cccgagtggc accgtcacca
      421 tcgccgggac ccgctacgcc ttcaccgtcc gcgcctgcac cctcatcaac gggcagcact
      481 acctcgtcgg cgacggcgaa gccaccatcg ccctctccac cgtgctcctc atccagaccc
      541 ccgcccgcgg ctccttctcc gtcgacaacc ccaccgtctc cgtcagcggc aacaccctca
      601 cagcctccgg caccggcatc aacatcgccc tgcctcgaga gcgccctgac gtcaccctca
      661 cggccacctg caccggtctg gcaaactgat ccacatcccg cctgcttcga ggacgaatgc
      721 cccatgcccg acccgcaacc cccgccctac gaacgcatca gcacgaagca atacgaacgt
      781 ctcctccacg acctccaggt cgagctcgtc aaactccagc gcgacttcat ccgcgacggg
      841 gaacgcaccc tcgtcatcct cgagggccga gacgccgccg gcaaagacgg caccatcaag
      901 cgcatcaccg agcacctcag cccgcgcgag acccgcgtcg tcgcgctggg caaaccctcc
      961 gaccgcgacc agaccacctg gtacttccag cggtacgccc cccacctccc cgccgcgagc
     1021 gagctcgtcc tcttcaaccg ctcctggtac aaccgcgcag gcgtcgagcg cgtcatgcac
     1081 ttctgcaccg ccgaccagta cgaggagttc atgacctccg tcctcgagtt cgaacatatg
     1141 ctgatccgct caggcattcg cctatttaag tactacctcg acatatccaa ggccgagcag
     1201 aaggaacgcc tcgaagaccg ccgcaagagt ccgctcacac agtggaaaat cagctccctc
     1261 gacgagaaag ccctcgacct ctgggacgac tactccgagg cacgcaatga catgttgctt
     1321 cgtacccggg acccgatcgc tccgtggatc gtcgtgcgtg ccaaccacaa gaagacggct
     1381 cgcgtccagc tcatccagga tctgctctcc cgcctcgact acgaggacaa ggacacgtct
     1441 ctcctcaagc ccgacccgtc ggtcgtcttc gagttcgacc ccgtgcacct cgaaaacggc
     1501 atgctcgagc cctgacgtcg acgcattagc acagatgttt
//
LOCUS       DAORPG010000526         1540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_11040_length_1540_cov_2.494276, whole genome shotgun sequence.
ACCESSION   DAORPG010000526 DAORPG010000000
VERSION     DAORPG010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11040_length_1540_cov_2.494276"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PLX85_09950"
     CDS             <1..771
                     /locus_tag="PLX85_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRC63527.1"
                     /translation="DPAGGLGGPWAMASGTASIDAGGVIVDRTLASSEGLDVGDTLTL
                     AGQQFTVQGLSRGTNALAGKVVFVTRDVAARMVGTEGAYSYLLVQADHGVSADEVAAR
                     LRTALPNTSVMTRAEFSRNERDLLTGLFVTPVNVMAVIGMLVGIMVVGLTIYTVASER
                     LRDFGVLKAIGASNRYVYLVILRLGLLVGMIGFVVGLSAGYLVKPLVEYFVPDLGIAY
                     RPEFILAVLGIAAGMSLLSALLPVYRIAGVDPKEVFKA"
     gene            768..1478
                     /locus_tag="PLX85_09955"
     CDS             768..1478
                     /locus_tag="PLX85_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC63528.1"
                     /translation="MSAPLELTGVRKVYGHPPSEVVAVDDVTLAVPEGRVVVVLGPSG
                     SGKTTLLSIAGCLLSPTEGSVRIVGNEVTSMSERQLPAVRLRHIGFVFQTFNLLDPLT
                     ALENVLMPMNLAGRGDARARSRARELLVELGLEHRVGSRPAELSAGERQRVAIARALA
                     NEPALVLADEPTANLDSASGQRVMRLLSGLVRAGAAKALVVVTHDQRVLEFADETYWM
                     EDGRLRRDGSLGVAGAAS"
BASE COUNT          222 a          482 c          583 g          253 t
ORIGIN      
        1 gaccccgcag gggggctcgg gggcccgtgg gcgatggcca gcggtaccgc cagcatcgac
       61 gcggggggtg tcatcgtgga ccggacgctg gcgagcagcg agggcctcga cgttggagac
      121 acgctcacgc tcgcgggaca gcaattcacg gtgcagggcc tgtcgcgagg gacgaacgcg
      181 ctcgccggca aggtcgtctt tgtgacgcgc gacgtggctg ctcgcatggt aggcacggag
      241 ggcgcgtaca gctacctgct cgtgcaggcc gaccacgggg tgagcgccga cgaggtggca
      301 gcccggctgc gcacggcgct gcccaacacc tcggtcatga cgcgcgcgga gttctcgcgg
      361 aacgagcgcg acctgctgac cgggttattc gtcacgcccg tgaacgtgat ggcggtcatc
      421 gggatgctcg tcggcatcat ggtcgtcggg ctcaccatct atacggtcgc ttcggaacga
      481 ctgcgtgact tcggcgtact gaaagcaatc ggcgcctcga accggtacgt gtacctcgtg
      541 atcctgcggc tggggttgct cgtggggatg atcgggttcg ttgtcggcct cagcgcgggc
      601 tacctcgtga agccgctggt cgagtacttc gtacctgatc tcgggatcgc ctaccggccg
      661 gagttcatcc tcgcggtgct cggcatcgcg gcggggatga gcctgctgtc tgcgctgctg
      721 ccggtgtacc gcatcgcggg ggtggatccc aaggaagtgt tcaaggcgtg agcgcgcctc
      781 tcgagctgac cggggtgcgg aaggtgtacg ggcacccgcc gtccgaggtg gtcgcagtcg
      841 acgacgtgac gctggccgta ccggaggggc gcgtggtcgt cgtcctcggg ccgtccggga
      901 gcgggaagac gaccctgctg tcgatcgccg gctgcctgct ctctccgacc gaggggtccg
      961 tgcggatcgt gggcaacgag gtcacctcga tgtccgagcg ccagctaccc gccgtccggc
     1021 tgcgtcacat cggcttcgtg ttccagacgt tcaacttgct cgacccgctg acggccctcg
     1081 agaacgtact catgccgatg aacctcgctg gtcgcggcga cgcgcgggcg cgttcgcggg
     1141 cgcgcgagtt gctcgtggag ctcggactgg aacatcgcgt cgggagccgg ccggcggaac
     1201 tcagcgcggg cgagcggcag cgggtagcga tcgcacgggc gctggcgaac gagccggcgc
     1261 tggtgctggc cgacgagccg accgcgaacc tcgactctgc ttcggggcag cgagtgatgc
     1321 ggctgctgtc cggcctcgtg cgcgcggggg cggcgaaggc actggtcgtc gtgacgcacg
     1381 accagcgggt gctcgagttc gcggacgaaa cgtactggat ggaagacggg cggctgcgcc
     1441 gcgacgggtc gctcggggtg gcaggggccg cctcgtaagt ctcgaaaacg caaacgcgga
     1501 gggacgcacg tccctccgcg ttccgtatgt agccgagccg
//
LOCUS       DAORPG010000527         1537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_11068_length_1537_cov_2.662618, whole genome shotgun sequence.
ACCESSION   DAORPG010000527 DAORPG010000000
VERSION     DAORPG010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11068_length_1537_cov_2.662618"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..590
                     /locus_tag="PLX85_09960"
     CDS             105..590
                     /locus_tag="PLX85_09960"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020639309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRC63529.1"
                     /translation="MTNEEWIQRRLLGEGRVSRFGVLSERLDEAGVAHFEVVCPPEYE
                     GGPGVAHGGWTAAVFDEVLGHVPLLHGHYTVTASLSVEFKRPVPVGVPLAATARAERV
                     EGRKWFLAGELALASSGAVLATASGLWIAREHAAHFEGFERWLAAQADSGQDGAGGAS
                     S"
     gene            complement(639..1127)
                     /locus_tag="PLX85_09965"
     CDS             complement(639..1127)
                     /locus_tag="PLX85_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HRC63530.1"
                     /translation="MKYSMSIVGVIAAAGLLAAACGGGSSDKTSTPGASSSSDKTATE
                     VAVTEKEWSVVPASASVPAGEVHFTVKNTGATAHEFVVIKATDDPAKLPTYQASDKVA
                     EGHAVGDVNEDKVDSVGELEDLAAGSSKDGTFKLSAGKYVLICNLPAHYGQGMRAAFT
                     VN"
     gene            1233..>1537
                     /locus_tag="PLX85_09970"
     CDS             1233..>1537
                     /locus_tag="PLX85_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC63531.1"
                     /translation="MRAQVTVITLAVADLERAVTFYRDGLGWATEGIIGTEFEYGAVC
                     FFDLEGGGRLALWPRESLAHDSALPEGAPNATELSLGHNVGSRAEVDVVREEARRAGA
                     "
BASE COUNT          204 a          458 c          633 g          242 t
ORIGIN      
        1 ggcgggttct gggcggatgg ggcggctcgg atcgaggata cgacggcccg gctgtgcggg
       61 ccgccctcgc ggggcgttgg gcgggccgcc gtatgatgct cggcatgaca aacgaggagt
      121 ggattcaacg gcggttgcta ggcgagggac gggtgagccg gttcggagtg ctcagcgagc
      181 gcctcgatga ggcgggtgtc gcgcacttcg aggtcgtctg tccgccggag tacgaaggcg
      241 ggccgggcgt ggcgcacggc gggtggacag cggcggtgtt cgacgaagtg ctggggcacg
      301 tgcccctcct gcacgggcac tacacggtga cagcgtcgct ctcggtggag ttcaagcggc
      361 cggtgccggt gggcgtgccg ctggcggcga cggcgcgcgc ggagcgggtg gaggggcgga
      421 agtggttcct ggccggggag ctggcgctgg cgtcctcggg ggcggtgctg gcgacggcgt
      481 ccggcctgtg gatcgcgcgc gagcatgcgg cgcacttcga ggggttcgag cgctggctgg
      541 ccgcgcaggc ggacagcgga caggacggcg ctggaggagc gtcctcgtga gggcgcggtc
      601 acggcgcgac cacgccctcg gaggagccgt cgttcgcgtc agttcaccgt gaacgcggca
      661 cgcataccct gtccgtagtg ggcggggagg ttgcagatga ggacgtactt gccggcgctg
      721 agcttgaagg tgccatcctt cgacgagccg gccgcgaggt cctcgagctc gccgacggag
      781 tcgaccttgt cctcgttgac gtcaccgacg gcgtggccct cggcgacctt gtcggaggcc
      841 tggtaggtgg gcagcttcgc ggggtcgtcg gtcgccttga tgacgacgaa ctcgtgcgcg
      901 gtcgccccgg tgttcttgac ggtgaagtgc acctcgccgg ccggaaccga ggcggaggcc
      961 gggacgaccg accactcctt ctcggtgacg gcgacctcgg tggcggtctt gtcggacgag
     1021 ctggacgcgc ccggcgtgct ggtcttgtcg ctgcttcctc caccacaggc cgccgccagg
     1081 aggcctgcgg ccgcgatgac gccgacgatg ctcatgctgt acttcatcgt tcccctctct
     1141 gaaaccttca tgcgcatcag gagcatattc ccgcgcgcgg tactgcggga gggagcggcc
     1201 tcgagaacgt gggcatacct gaggaggtgg caatgcgagc gcaagtgacg gtgatcacgc
     1261 tggcggtcgc cgacctcgag cgcgcggtga cgttctaccg cgacggcctg gggtgggcga
     1321 ccgagggcat catcgggacg gagttcgagt acggcgcggt gtgcttcttc gacctcgaag
     1381 gtggggggcg gctggcgctc tggccgcggg agagcctggc gcacgactcg gcgctgccgg
     1441 agggcgcgcc gaacgcaacg gagctgtcgc tgggccacaa cgtcgggagc cgcgcggagg
     1501 tggacgtggt gagggaggag gcgcggcgcg ccggggc
//
LOCUS       DAORPG010000528         1535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_11096_length_1535_cov_2.652027, whole genome shotgun sequence.
ACCESSION   DAORPG010000528 DAORPG010000000
VERSION     DAORPG010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11096_length_1535_cov_2.652027"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PLX85_09975"
     CDS             <1..838
                     /locus_tag="PLX85_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63532.1"
                     /translation="LANARQHVDDCLAISDSNSRTLAIQALATALAGMIEAAGGQSAR
                     GERLCGDGVARLDAMALHWMLAIALEWRGEAALRAGHDGLAETSWERSVSLFRELGDR
                     WLQAAPLARLGDLALDRGDLARAGELLAESIALLRAVGTGAGSAQVLASLGRLARAEG
                     RSRDARMLAAESLRAASASGSWLEVDLGLSLLASLCSDGPHPADALEASRLLGAAARI
                     RTAAGPLASSTIQRGDAHLAVLLRTTLGTAAFEAAWAEGHASTTVEAVALAIGLAGNA
                     DE"
     gene            825..>1535
                     /locus_tag="PLX85_09980"
     CDS             825..>1535
                     /locus_tag="PLX85_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63533.1"
                     /translation="MQTNSAATWIPRAAERRARAFDAACAHARTHLEDAQQCDLVLSI
                     LRAAAERAAAPEAPALFPLTVHVPLAVHAAFEGHDEPALPIAAAGLLVLLGADLLDTL
                     IDDDPFSGPLTPAERLLAGAGLVGPLPTLLLLSMDVPPDIAREAARDLNEAVVTMGAG
                     QLADLRSPAAPYTSLPGVVETAVFKSSPLVAALCRIAARLAGGTPGFDAQGRLLCTLP
                     GWGHDHVDSMVVGDLDPLR"
BASE COUNT          184 a          604 c          500 g          247 t
ORIGIN      
        1 gctcgccaac gctcgccagc atgtggatga ctgcctcgcg atcagcgata gcaactcccg
       61 cactctcgcg atccaggccc tcgcgacggc gctcgccgga atgatcgagg ccgccggcgg
      121 gcagagcgcc cgcggcgaac ggctgtgcgg cgatggcgtc gcacggctcg acgccatggc
      181 gttgcactgg atgctcgcga tcgcgctcga atggcgtggc gaagccgctc ttcgtgccgg
      241 ccatgacggc ctggcggaga cctcctggga acgctccgta tcactctttc gcgaactcgg
      301 cgatcgctgg ctccaggctg cgcccctcgc ccgcctcggc gatctcgccc tcgaccgcgg
      361 cgatctcgcg cgcgctggag agctgctcgc cgagagcatc gctctgctcc gcgccgtcgg
      421 cacaggcgcg ggctccgccc aggtgctcgc cagtctcggt cgcctcgcgc gagcggaagg
      481 acgttctcgc gatgctcgca tgctcgccgc cgaatccttg cgagctgcca gcgcctctgg
      541 cagctggctc gaggtcgatc tgggcctgtc gctcctggcc tccctttgct ccgacggacc
      601 gcacccagcg gacgccctcg aggcctccag gctgctcggt gcagccgcgc gcatccgcac
      661 cgccgccggg ccgctggcct cctccaccat ccagcgcggt gacgctcatc tcgcggtcct
      721 cctcaggaca acactcggta ccgcggcctt cgaggccgcg tgggcggagg gccacgcctc
      781 gaccaccgtc gaggccgtgg cgctggcgat cgggttggcc ggcaatgcag acgaatagcg
      841 ccgccacctg gatacctcgg gcggccgaac gccgtgcgcg cgcgtttgac gccgcttgcg
      901 cccacgctcg gacccacctc gaggatgcgc agcagtgcga cctcgtcctc tccattctcc
      961 gcgccgccgc ggaacgcgcc gccgcgccgg aggcccctgc gctgttcccg ctcacggtcc
     1021 acgtgccgct cgcggtgcac gcggctttcg agggccacga cgagcccgcg cttcccatcg
     1081 ccgccgccgg gctcctcgtc ctcctgggag ccgacctgct cgacacgctg atcgatgatg
     1141 accccttcag cggtccgttg acgcccgccg aacggttgct cgccggtgcc gggctggtcg
     1201 gcccgctccc gacgctcctg ctgttatcga tggacgtccc gcctgacatc gcccgcgaag
     1261 cggctcgtga cctgaacgag gccgtcgtca ccatgggtgc cggacagctt gcggacctcc
     1321 gctcaccggc cgccccgtac acgtcccttc cgggtgtcgt cgagaccgcc gtcttcaaga
     1381 gctcgcctct ggttgcggca ctctgccgga tcgcggcccg cctcgccggc ggcacgccgg
     1441 gcttcgatgc gcaggggcgc ctgctctgca ccctgccggg ctgggggcac gaccatgtcg
     1501 actcgatggt cgtcggcgac ctcgatccac tgcgc
//
LOCUS       DAORPG010000529         1534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_11108_length_1534_cov_2.759973, whole genome shotgun sequence.
ACCESSION   DAORPG010000529 DAORPG010000000
VERSION     DAORPG010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11108_length_1534_cov_2.759973"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PLX85_09985"
     CDS             <1..341
                     /locus_tag="PLX85_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRC63534.1"
                     /translation="IRDALARRGIPPERQARPWYFPTPAEYATLLERHGFEVRALWHF
                     ERFTQLVPGDHALADWLTMFASPFTGDLTAEDRAAFHAEVEDATRHALYVDGRWHVDY
                     RRLRFVAVRI"
     gene            436..1341
                     /locus_tag="PLX85_09990"
     CDS             436..1341
                     /locus_tag="PLX85_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020520187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HRC63535.1"
                     /translation="MHQNDLDDRHDRGLQYDLATLYRRRRVLQLFGGVALAAMVGCSN
                     DDDNGDGATSTVAPELTATATAAGTTATRVATAASTLVAPIPVSPTPLSACTRIPAET
                     AGPFPGDGSNGPNALAQSGIVRSDLRASFGASTKVSAGAPLEVELTVVDTKNACRPLA
                     GAALYLWHVDAEGRYSMYSAGATAENYLRGVQVADANGVVRFTTVFPGAYTGRWPHIH
                     FEVYPSLDAATSSANKLATSQLALPEDVCRQVYSGSGYPGSLANLAQTPLARDTVFAD
                     GVTLQMATVSGSLNAGYRASLVVGI"
BASE COUNT          212 a          638 c          444 g          240 t
ORIGIN      
        1 ccatccgtga cgccctcgcg cggcgcggca tcccgcccga gcgccaggcc cgcccctggt
       61 acttcccgac gcccgccgag tacgccaccc tcctcgaacg ccacggattc gaggtgcgcg
      121 ccctctggca tttcgagcgc ttcacccagc tcgtcccggg cgatcacgcc ctcgccgact
      181 ggctcacgat gtttgcctcg cccttcacgg gcgacctaac cgccgaggac cgcgccgcct
      241 tccacgccga ggtggaggac gcgactcgcc atgctctcta cgtcgacggc cgctggcacg
      301 tcgactaccg ccggctgcgc ttcgtggcgg tgcgcatcta gcccgatccg cgctgccttg
      361 cgttcgcgtg agacgatcga acgcagttca tcacctcgtg caccgctccc cgctcgcctg
      421 acgaggactg accccatgca ccagaacgac ctcgacgacc gtcacgaccg agggctccag
      481 tacgacctcg ccaccctcta ccggcgtcgc cgcgtgctcc aactgttcgg cggcgtcgcg
      541 ctcgccgcga tggtcggctg ctccaacgac gacgacaacg gcgacggcgc cacctccacg
      601 gtggctccgg agctgaccgc gaccgccacg gccgccggca ccaccgctac gagggtggcc
      661 accgccgctt ctacgctggt cgcgccgatt cccgtatcgc cgacgccgct ctcggcctgc
      721 acccgcatcc ccgccgagac ggctggcccg tttccgggcg acggctccaa cggcccgaac
      781 gctctcgccc agagcggcat cgttcgctcc gacctccgcg cgagcttcgg cgcctcgacg
      841 aaggtctccg ctggcgcacc actcgaggtc gaactcaccg tggtggacac aaagaacgcc
      901 tgtcggcccc tcgccggcgc cgccctctac ctctggcacg tcgacgccga aggccgctac
      961 tcgatgtact ccgccggcgc caccgccgag aactacctcc gaggcgtaca ggtcgccgac
     1021 gccaacggcg tcgtccgctt caccaccgtg tttcccggtg cctacacggg ccgctggccc
     1081 cacatccact tcgaggtcta tccgagcctc gacgcggcca cctcgtccgc caacaagctg
     1141 gccacgtccc agctcgctct ccccgaggac gtctgccgcc aggtctactc tggcagcggc
     1201 taccccggta gcctcgccaa ccttgcgcag acgccgctcg cgcgcgacac cgtcttcgcc
     1261 gacggcgtca cgctccagat ggcgaccgtc tccggcagcc tgaacgccgg ctatcgtgcc
     1321 tccctcgtcg ttggtatctg agcggtaagc cacgcccggc gcaaacagaa cggcggcccg
     1381 caggccgccg ttcctggcac tgactcgaac ccgccgctac gccccgcggt agctgctgtt
     1441 cgaaccgctc gagctgccgc tgccctgatc gccgttctgg tcatgcgggc agttgtggtc
     1501 ctgctgctgt ccgctcccgc cgttcgccgg cgcg
//
LOCUS       DAORPG010000530         1533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_11117_length_1533_cov_3.602165, whole genome shotgun sequence.
ACCESSION   DAORPG010000530 DAORPG010000000
VERSION     DAORPG010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11117_length_1533_cov_3.602165"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="PLX85_09995"
     CDS             complement(<1..812)
                     /locus_tag="PLX85_09995"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRC63536.1"
                     /translation="MTLTRPRSLSPSGLRALAGVARQHLDRGLHSGAQLAVYRDGELQ
                     LDERLGTAAGEDARMLWFSATKPLTAVCILMLVERGTIHLDRPIAEVWPEFGQGGKAS
                     CTPRHVLTHRGGFPVFPREFDWVDIGDWDAVIAAVAGLEAQWEPGTAVGYHPVTYGFA
                     LGELIRRADGRMPRDFMRDELFEPLRMDASLGVDEAGLARVVQVEAMSEVTFDDPEGS
                     ERRTSEMVERFNRPTTLQAQIPAANAIGTAEALARFYAMLERGGALGGRRIL"
     gene            889..1317
                     /locus_tag="PLX85_10000"
     CDS             889..1317
                     /locus_tag="PLX85_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRC63537.1"
                     /translation="MVSLESLERDVSRSGYRVTGPRRELLATLQEMGDHFTAEGLVAA
                     VPGVGRATVFRTLRLLQDIGTLCQVVLDDGTLQYRLTSGGHHHHVVCSRCGAVNDFSA
                     CDIQGLLDEIAERTGYVIDGHRLEVYGRCADCKEVLTSET"
BASE COUNT          242 a          562 c          499 g          230 t
ORIGIN      
        1 tcgaggatgc gcctcccgcc gagcgcgccc ccgcgctcga gcatcgcgta gaagcgcgcc
       61 aacgcctcgg ccgttccgat ggcgttcgca gccggaatct gtgcctgcag cgtagtcgga
      121 cggttgaacc gttccaccat ctccgaggtg cgtcgctcgc tcccctcggg gtcgtcgaac
      181 gtgacttcgg acatcgcctc gacctgcacc acgcgagcca gtccggcctc atcgaccccg
      241 agcgatgcgt ccatgcgcag cggctcgaac agctcgtccc gcatgaagtc ccgtggcatg
      301 cgcccatcgg cgcgccggat cagctcgccc agtgcgaacc cgtaggtcac ggggtggtac
      361 ccgaccgcgg tccccggttc ccattgcgcc tcgaggccgg cgaccgccgc gatgaccgca
      421 tcccagtcgc cgatgtccac ccagtcgaac tcgcgaggga agaccgggaa gccgcctctg
      481 tgcgtcagca cgtgcctcgg cgtgcaggat gccttgccgc cctgcccgaa ctcgggccag
      541 acctccgcaa tcggccggtc gagatggatc gtcccgcgct cgaccagcat caggatgcac
      601 acagcggtca gcggcttcgt cgccgagaac cagagcatgc gcgcgtcctc accggcagcc
      661 gtcccgaggc gctcgtcgag ctgcaactca ccgtcgcggt acaccgcgag ctgggctccc
      721 gaatgtaggc cccgatcgag gtgctggcgg gcgacgccag ccagcgcacg aaggcccgaa
      781 gggctgaggg aacggggccg ggtgagcgtc acccgtccat tctaggcggc gcgcccgccg
      841 gagcggcgcg cgttgacagc ccgtttacgc gaggaagacc atgtccgcat ggtcagcctc
      901 gagtccctcg aacgggatgt ctcgcgcagc ggataccggg tcacgggccc tcgacgcgaa
      961 ctccttgcga cgctccagga gatgggcgac cacttcaccg cggagggcct cgttgccgcc
     1021 gtccccggag tgggacgcgc caccgtgttc cgcacgctgc ggctgctgca ggacatcggc
     1081 acgctgtgcc aggtcgtcct cgatgacggc acgctccagt accggctcac gagcggcggc
     1141 caccaccatc acgtcgtctg ctcgcgctgc ggagccgtga acgacttctc ggcctgtgac
     1201 atccaggggc tgctcgacga aatcgccgag cgtacgggct acgtcatcga tggccaccgc
     1261 ctcgaggtgt acggccgctg tgcggactgc aaagaggtgc tcacctccga aacgtagccc
     1321 gcacgagcga cggctgcgcc ggtacccaac catcgtcatt acactacgcg tcgaaccgct
     1381 ggaaggagcc cgcctccggc catgcagacg cgtccccgaa accaggcagg cgtcgacgtt
     1441 gtcgtccgcc tgctcgcggt gctgacactc gtgctcgccg cgaactacgt cgcgtggcgc
     1501 tggacggact ccgtggcgtg gggcgcctgg cct
//
LOCUS       DAORPG010000531         1532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_11125_length_1532_cov_4.305349, whole genome shotgun sequence.
ACCESSION   DAORPG010000531 DAORPG010000000
VERSION     DAORPG010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11125_length_1532_cov_4.305349"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..836)
                     /gene="tpiA"
                     /locus_tag="PLX85_10005"
     CDS             complement(69..836)
                     /gene="tpiA"
                     /locus_tag="PLX85_10005"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008733154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRC63538.1"
                     /translation="MPRRPIVAGNWKMHLNRSAAAELVSALRGGLDGLAGIDVVLCPP
                     APWLAETADHLRGSSLEVGAQNVYWEPHGAFTGEVSAAMLTGLVDYVIIGHSERRHVF
                     GETDEDTAKKLDAVLGLGMQPILAVGELREEREAGQTPAILERQLRAAFANVDRLPEG
                     FVVAYEPVWAIGTGLTATPEIAQQACAHVRRIVAERFGDGPAETCRVQYGGSVNAENA
                     AALAAQSDIDGLLVGGASLNAEAFTAICRAVAATKGS"
     gene            complement(838..>1532)
                     /locus_tag="PLX85_10010"
     CDS             complement(838..>1532)
                     /locus_tag="PLX85_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="catalyzes the interconversion of 3-phosphoglycerate
                     and 2-phosphoglycerate; this enzyme does not require the
                     cofactor 2,3-bisphosphoglycerate as a phosphate donor;
                     BPG-independent PGAM; aPGAM; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HRC63539.1"
                     /translation="LDPVTPRFAADALAYRHVDVKLDRAARTAELVNQFVADAQRVLS
                     KRDQANGMTLRGWAKRPDLPQMPELWKLRAAAVAVYPMYRGLARLVGMDVLDAGANME
                     SQIEALRRHWNDYDFFFLHYKYTDSAGEDGDFARKQQAIRDFDACVPALQALGPDVIV
                     VTGDHSTPAMMASHSWHPVPFLLRGSNVRIDAANRFNEVECARGGLGTFPAKEALPLA
                     FAHAGRMTKYGA"
BASE COUNT          228 a          469 c          605 g          230 t
ORIGIN      
        1 ggcgacttca gtcttgccgg aggcagtggc tccgacgagg gcgacgaggt ggcgtcgcga
       61 gggtgcggtc acgagccctt cgtggcggcg acggcgcgac agatcgcggt gaacgcttcg
      121 gcgttcagcg aagcgccgcc gacgagcaag ccgtcgatgt cggactgggc cgcgagcgcc
      181 gcggcattct cggcgttgac cgaaccgccg tactgcacgc ggcacgtctc ggcggggccg
      241 tcgccgaagc gctcggcgac gatgcgacgc acgtgcgcgc aggcctgctg ggcgatctcg
      301 ggggtggccg tgagaccggt gccaatggcc cagaccggct cgtaggcgac gacgaaaccc
      361 tcggggaggc ggtcgacgtt cgcgaaggcg gcacggagct ggcgttcgag gatggcgggc
      421 gtttgtccgg cctcgcgctc ctcgcggagc tcgccgacgg caaggatggg ctgcatgccg
      481 aggccgagga cggcatcgag cttctttgcg gtgtcctcgt ccgtctcgcc gaagacgtga
      541 cggcgttcgg agtggccgat gatgacgtag tcgacgagcc cggtgagcat ggctgcgctc
      601 acctcgccgg tgaaggcgcc gtgcggctcc cagtacacgt tctgcgcgcc gacctcgagg
      661 ctgctgccgc ggaggtgatc cgcagtctcg gccagccagg gagcgggcgg acagaggacg
      721 acgtcgatcc cggcgaggcc gtcgaggcca cctcgaaggg cggagaccag ctcagcggcg
      781 gccgagcggt tgaggtgcat cttccagttg ccggcaacga tggggcggcg gggcatgtca
      841 ggctccgtac ttcgtcatgc gcccggcgtg ggcgaaggcg agcgggagcg cttccttcgc
      901 ggggaaggtg ccgagcccgc ctcgggcgca ctcgacctcg ttgaatcggt tggcggcatc
      961 gatgcggacg ttgctgccgc gcaggaggaa tgggaccggg tgccaagagt gggacgccat
     1021 catggccggc gtcgagtggt cgccggtcac gacgatgacg tccgggccga gggcctggag
     1081 ggccgggacg caggcgtcga agtcgcggat cgcctgctgt ttgcgggcaa agtcgccatc
     1141 ctcgccggcg gagtcggtgt acttgtagtg caagaagaag aagtcgtagt cgttccagtg
     1201 tcgccggagc gcctcgatct gggactccat gttggcgccc gcgtcgagga catccatgcc
     1261 gacgaggcgg gcgagccctc gatacatggg gtagaccgcg acggcggcgg cgcggagctt
     1321 ccacagctcg ggcatctggg gaaggtcagg gcgcttcgcc cagcctcgaa gggtcatgcc
     1381 gttcgcctga tcccgcttgg agagcacgcg ctgggcgtcg gcaacgaact ggttcaccag
     1441 ctcggcggtg cgggccgcgc gatcgagctt gacgtcgacg tggcggtagg cgagcgcgtc
     1501 ggcggcgaac cgcggcgtga ccgggtcgag gg
//
LOCUS       DAORPG010000532         1530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_11150_length_1530_cov_2.159322, whole genome shotgun sequence.
ACCESSION   DAORPG010000532 DAORPG010000000
VERSION     DAORPG010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11150_length_1530_cov_2.159322"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PLX85_10015"
     CDS             <1..502
                     /locus_tag="PLX85_10015"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HRC63540.1"
                     /translation="LKYSSAPSNTTGWIVIGTSLARDTVTTRDGASYKAVSSGVNGLL
                     CSGATYVPRVAAGHQYTLSFWVNTETASRNILPAMVWKPNSDGSGTTISTTTSTVAMS
                     VGAWKYIALTGTAPAGALSAVLTLYVSGVATYYVDEIQFEAGEFATPHIATDGATKTR
                     VATRHG"
     gene            516..902
                     /locus_tag="PLX85_10020"
     CDS             516..902
                     /locus_tag="PLX85_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63541.1"
                     /translation="MPQVKFNRPVSINLTGYDREAVTLEAGVHNVPERVAGWVRRNPS
                     VGDVLDAAAASAPAADGVDFSRLTVAELRAAASAEVIAGFAGMKKAELGAALEAEGQP
                     PGAEDVAADGADETNDESAADEGEED"
     gene            905..>1530
                     /locus_tag="PLX85_10025"
     CDS             905..>1530
                     /locus_tag="PLX85_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63542.1"
                     /translation="MALLDAYATKDEYRAAVGSKATGTDTTLDAQLTAMTRQTERSLQ
                     VAPGGFNSHAGTYTFDAHGGQVLWLRDSADLGYFLQSITADSCGIDIEEDGTFDYYTL
                     DLGDAWLRGLPEHAATFSEPYIALQLLAHRTNAEPTEWPDQPASVRIAGTWGWAAVPR
                     VIKELVIHRTHELREALKGGALEAMTNFEGTTAFPAGGAGVPMGQRTF"
BASE COUNT          282 a          487 c          539 g          222 t
ORIGIN      
        1 cctgaagtac agcagcgcgc cgagcaacac cactgggtgg atcgtcatcg ggacgagcct
       61 cgcgcgtgac accgtcacca ctcgggatgg cgcctcctac aaggcagtct cgagtggtgt
      121 caacggactg ctctgcagcg gtgcgacgta tgtacctcgg gtggccgcgg gtcatcagta
      181 cacgctgtcg ttttgggtca acacagagac ggcatcgcga aacatcctcc ccgcgatggt
      241 ctggaagccg aactccgacg ggtcgggcac gaccatcagc acgacgacga gcaccgtggc
      301 gatgagcgtc ggcgcatgga agtacatcgc gcttactggc accgcgccgg ccggggcgtt
      361 gagcgccgtc ctgacgctct acgtaagcgg tgttgcgacc tactacgtgg acgagatcca
      421 gttcgaggcg ggggagttcg cgacgcccca catcgcgacg gatggggcga cgaagactcg
      481 tgtcgccacc cggcacggct aacgaggagg aaacgatgcc gcaggtgaag ttcaaccggc
      541 cggtcagcat caacctcacg gggtacgacc gcgaggccgt gactctcgag gcgggggtac
      601 ataacgtgcc agagcgcgtc gccgggtggg tccggcgcaa cccctctgtg ggagacgtcc
      661 tagacgccgc ggcggcaagc gcccctgccg ccgacggcgt cgacttctcg cggctcacgg
      721 tcgcggaact gcgagcggcg gcgtccgctg aggtgatcgc gggcttcgcg ggtatgaaga
      781 aggcggagct cggggctgcg cttgaggccg aagggcagcc gcccggagct gaggacgtcg
      841 ctgcggacgg ggctgacgaa accaacgatg agagcgccgc ggacgagggc gaggaagact
      901 gacagtggcg ctcctcgacg cctacgcgac gaaagacgag tatcgcgccg ccgttgggag
      961 caaggccacc gggacggata cgacgctcga cgcgcaactc acggccatga cgcgacagac
     1021 ggagcgttcg ctgcaggtcg ctccgggcgg gttcaattcg catgccggca cctacacctt
     1081 cgacgcgcac ggtggacagg tgctctggct gcgcgacagc gcggatctcg ggtacttcct
     1141 ccagtcgatc accgccgaca gctgcggcat cgacatcgaa gaagacggca cgttcgacta
     1201 ctacacgctg gacctcggcg acgcctggtt gcgcggcttg ccggagcacg ccgccacctt
     1261 cagtgagccg tacatcgcgc tgcagttact tgcccaccgc acgaacgccg agcccaccga
     1321 atggcctgat cagcccgctt cagtgcggat cgcgggcacg tgggggtggg ccgccgttcc
     1381 acgcgtcatc aaagagctgg tcatccaccg gacgcacgag ctgcgtgagg cgctgaaggg
     1441 tggggcgctg gaggccatga cgaacttcga ggggacgacc gcctttccag cgggcggagc
     1501 gggcgtgccg atggggcagc gcacgttctg
//
LOCUS       DAORPG010000533         1523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_11229_length_1523_cov_2.483651, whole genome shotgun sequence.
ACCESSION   DAORPG010000533 DAORPG010000000
VERSION     DAORPG010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11229_length_1523_cov_2.483651"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PLX85_10030"
     CDS             <1..366
                     /locus_tag="PLX85_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC63543.1"
                     /translation="QVQHGGVQRERHAIAEHYIESEVMFNYSFRIISMQARGIVPNYE
                     ASTSKMYHSELVQRVSRTAIKAYGLYSNVWDPDEKWAAQKGLFTQSNVHSVVGTIAGG
                     SSEIQRNIIATRGLGLPRG"
     gene            480..1268
                     /locus_tag="PLX85_10035"
     CDS             480..1268
                     /locus_tag="PLX85_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRC63544.1"
                     /translation="MDFETIRFERDGHVATVTLNRPERLNAFNEQMSFDLSHAWSEVR
                     DDPAIRVAILTGAGDRAFCSGVDVAAAPEADPLATRPWIARMTARGNQCYKPVIVAVN
                     GLAAGGAAYWIGECELAIAADTATFFDPHVDRGMVPLEEPMIYARRLPLMPVMRMILL
                     GTSDRMDARTALAAGLVTEVVPAAGLMATARSYAEIIASKSPQGVQRAVRVLWETKHL
                     PLDDALEVAWREIVEHSAVSTDLVEGKRAFLEKRAPDWDHPAEG"
     gene            complement(1265..>1523)
                     /locus_tag="PLX85_10040"
     CDS             complement(1265..>1523)
                     /locus_tag="PLX85_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63545.1"
                     /translation="CLIAPMLPMRWAMRGALLRFHTVAREHGVEPQGTWMSRAAHEIF
                     LLVEAPNVHAIEEILVDAGLVGLTRSQVLPVLPAAQVIGAS"
BASE COUNT          246 a          564 c          474 g          239 t
ORIGIN      
        1 caggtgcagc acggcggtgt gcagcgcgag cgccacgcga tcgcggagca ctacatcgag
       61 tcggaagtga tgttcaacta ctccttccgc atcatctcca tgcaggcccg gggtatcgtc
      121 ccgaactacg aggcgtctac ctcaaagatg taccactccg agctggtgca gcgcgtctcg
      181 cgtacggcca tcaaggccta tggcctctac agcaacgtct gggacccgga cgagaagtgg
      241 gctgcacaga agggcctctt cacgcagtcc aatgtccact ccgtcgtcgg caccattgcc
      301 ggcggttcga gcgagatcca gcgcaacatc atcgctaccc gtggtctcgg tctgccccgc
      361 ggctagcgag gcaggtgccc catacacgag agcggcccac ccgggccgct cttcgtattc
      421 gcgcccgcaa ggccgcggcg cgagtgctac catccctccc cgcgcacggg gaggccgcga
      481 tggacttcga gacgattcgc ttcgagcgcg atggccacgt cgccacggtt accctcaatc
      541 gccccgagcg cttgaacgcc ttcaacgagc agatgtcgtt cgacctcagt cacgcctggt
      601 ccgaggtgcg cgacgacccc gccatccgcg tcgccatcct cacgggcgcc ggcgaccgcg
      661 cgttctgctc gggcgtcgat gtcgccgccg cccccgaggc cgatcccctc gcgacccggc
      721 cctggatcgc ccgcatgacc gcccgcggca atcagtgcta caagccggtg atcgtcgccg
      781 tgaacggcct cgcggctggc ggcgccgcct actggatcgg cgagtgcgag ctcgcgatcg
      841 ccgccgatac ggctaccttc tttgacccgc acgtcgaccg cggcatggtt ccgctcgagg
      901 agccgatgat ctacgcgcgt cgcctgccgc tgatgccggt catgcgcatg atcctgctcg
      961 ggacgagtga tcggatggac gcccgcaccg cccttgccgc gggactcgtt accgaggtgg
     1021 tgccagccgc cgggttgatg gcgaccgcgc gcagttacgc cgagatcatc gcctcgaagt
     1081 caccgcaggg cgtgcagcgc gcggtccgcg tgctctggga gacaaagcat ctcccgctcg
     1141 acgacgccct cgaggtcgcc tggcgcgaga tcgtcgagca ctcagccgtc tccacggacc
     1201 tcgtcgaggg caagcgcgcc ttcctcgaga agcgcgctcc agactgggat caccccgccg
     1261 agggctagct cgcgccgatg acctgggcgg cgggcagcac gggcagcacc tggctgcgcg
     1321 tcagccccac cagccccgcg tcgaccagga tttcctcgat ggcgtgcacg ttgggtgcct
     1381 ccaccagcaa gaagatctcg tgtgccgccc ggctcatcca ggtcccctgc ggctccacac
     1441 cgtgctcccg cgcgaccgta tggaaccgca gcagcgcgcc ccgcatcgcc cagcgcatcg
     1501 gcaacatcgg cgctatcaag caa
//
LOCUS       DAORPG010000534         1517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_11305_length_1517_cov_3.088919, whole genome shotgun sequence.
ACCESSION   DAORPG010000534 DAORPG010000000
VERSION     DAORPG010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11305_length_1517_cov_3.088919"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="PLX85_10045"
     CDS             <1..758
                     /locus_tag="PLX85_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRC63546.1"
                     /translation="CFPKPKHLSWEAASAYMLVGATAYRMLMHWEPNVVHENEPVLIW
                     GGAGGLGSMAIQITKAAGGIPIAVVSEENKVEFCLQLGAKGVINRKDPAFTHWGRLPD
                     VDDAEAFNKWMQGARAFGAKYWEVLGERRNPAIVFDHPGEATIPTSIFMVDNGGMVVI
                     CAGTSGYNADVDLRYLWMRQKRLQGSHFASTEECAALNQLVIDGKVDPCLGEVYGFDG
                     VGLSHQAMYENRAVGNMAILVNAREKGLTTLPA"
     gene            923..>1517
                     /locus_tag="PLX85_10050"
     CDS             923..>1517
                     /locus_tag="PLX85_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63547.1"
                     /translation="MGQTHLRVPPSSIEGSALTNSTPADQPRRLPQPSGYILAAVAGV
                     LGAVLLGAGIYLSYSKYQGERERVLLDVAGQAQVVGARFDQFFRERFEYLDALAQSPL
                     VQTGAPAEVKAYFERIAGRGSTFSGGLSLIGTDGQLILLSNYPLDSPPVNLADRDYVQ
                     AVLRTQQPYFGNAILGRATGDPLLTVAVPVRNDRGEGL"
BASE COUNT          242 a          562 c          452 g          261 t
ORIGIN      
        1 agtgcttccc caagccgaag catctctcct gggaggccgc ctcggcctac atgctcgtcg
       61 gcgccaccgc ctaccgcatg ctcatgcact gggagccgaa cgtcgtccac gagaacgagc
      121 ccgtcctcat ctggggcggc gcaggcggcc tcggcagcat ggccatccag atcaccaagg
      181 ccgcgggcgg catcccgatc gccgtggtct ccgaggagaa caaggtcgag ttctgccttc
      241 agctcggcgc caagggcgtg atcaaccgca aggaccccgc gttcacccac tggggccgcc
      301 tccccgacgt cgacgacgct gaggcgttca acaagtggat gcagggcgcg cgcgccttcg
      361 gcgccaagta ctgggaggtc ctcggcgagc gccgcaaccc ggcgatcgtc ttcgaccacc
      421 ccggcgaggc caccatcccc acctcgatct tcatggtcga caacgggggc atggtcgtca
      481 tctgcgccgg cacctccggc tacaacgcgg acgtcgacct gcgctacctc tggatgcgcc
      541 agaagcgact ccagggctcg cacttcgcga gcaccgagga gtgcgccgct ctcaaccagc
      601 tcgtcatcga cggcaaggtc gacccgtgcc tcggtgaggt ctacggcttc gatggcgtcg
      661 gcctcagcca ccaggccatg tacgaaaacc gggccgtcgg caacatggcc atcctcgtca
      721 atgctcgcga gaagggcctc accaccctcc ccgcctagcg atcgtcctga cgctgaacaa
      781 gaacgcccgc gttgccgcgg gcgttcttgt tgtcttgacg tatcggccca ggacattgcg
      841 ggttcatcgc agtgttgtcg cgatttgacc tgttatcctc gtctcgcgag acgccctttg
      901 cggtgctttg ctatgcatat gggtggggca gacacatctc cgcgtccccc cttcatctat
      961 cgagggatct gccctgacga actccacgcc cgcggaccag cctcggcgct taccccagcc
     1021 gtccggctac atcctcgccg ccgtcgctgg cgtgctgggc gcggtattgc tcggcgccgg
     1081 catctacctc tcctacagca agtaccaggg cgaacgcgaa cgcgtcctgc tcgacgtcgc
     1141 cggccaggcc caggtcgtcg gggcgcgctt cgaccagttc ttccgcgagc ggttcgagta
     1201 cctcgatgcc ctcgcgcagt cccccctcgt gcaaacgggc gcgcccgccg aggtcaaagc
     1261 ctatttcgag cgcatcgccg gccgcggttc cacgttctcc ggcggactga gcctcatcgg
     1321 cacggacggc cagctcatcc tcctcagcaa ctacccgctg gacagcccgc cggtaaacct
     1381 cgccgaccgt gactatgtcc aggccgtcct ccgcacgcag cagccttatt tcggcaacgc
     1441 cattctgggc cgcgcgaccg gcgacccgct gctgaccgtc gctgtgccgg ttcgcaacga
     1501 tcgcggcgag gggctgc
//
LOCUS       DAORPG010000535         1509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate AMR_MDS_5401
            NODE_11392_length_1509_cov_2.725585, whole genome shotgun sequence.
ACCESSION   DAORPG010000535 DAORPG010000000
VERSION     DAORPG010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867618
            Sequence Read Archive: SRR1022361
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (wastewater metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Bacillati; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:10:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,012
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 19 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11392_length_1509_cov_2.725585"
                     /isolate="AMR_MDS_5401"
                     /isolation_source="Biofilm of wastewater treatment plant"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLX85_10055"
     CDS             <1..334
                     /locus_tag="PLX85_10055"
                     /inference="COORDINATES: protein motif:HMM:NF013962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRC63548.1"
                     /translation="GARTVTATHTPSGVRVVGIDDYVVDISPDAAQHVLMVENLDRPG
                     MIGRVGTTLGEWGVNVSYMSVGSRPSAREQALMVLGINRALSAGEIQTLLGLDNIFSA
                     RQLDLSQA"
     gene            complement(379..>1509)
                     /locus_tag="PLX85_10060"
     CDS             complement(379..>1509)
                     /locus_tag="PLX85_10060"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRC63549.1"
                     /translation="EASDAGYAQAAALLERWEREGALRRDERPALYVYEQAFEVHGGS
                     YVRRSFFARLRIHPHEDGIVRPHEATLTAAKEDRLRLLRATRTNVSPIFGMFEDAAGR
                     ARAVLDRIATAEPAFVAVDARGESHRLWPVTAAEDIDSLVTTVAVSNVTIADGHHRYH
                     TALNYLHEREAAGDASEADRYILAGLVPVDDPGLVVLPIHRLIRRESVPTDLLARLTD
                     LYDVERLDLPWSAEGVASLWARVQREVGSRVSFGAIGLEPQAFHVLTARSAEAIEAAM
                     PGAWSAASRALHVLILNETVLQPLLGLDAAQRASGAVGYTEDETEAWREVDAGAYGAA
                     FLVDHVHVRQIVAVADAGEVLPQKSTYFYPKLATGMVLNPLD"
BASE COUNT          239 a          505 c          545 g          220 t
ORIGIN      
        1 cggcgcgcgc accgtcacgg cgacgcacac cccgtccggt gtccgcgtcg tgggcatcga
       61 cgactacgta gtcgacatct cgcccgacgc ggcgcagcac gtactgatgg tcgaaaatct
      121 cgaccggccg gggatgatcg ggcgagtggg taccacgctc ggcgagtggg gcgtcaacgt
      181 ctcgtacatg tcggtcggtt caaggccgag cgcgcgagag caggcgctca tggtcctcgg
      241 gatcaaccgt gcgctatccg ccggcgagat tcagacgctg ctcgggctcg acaacatctt
      301 cagcgcgcgg cagctcgacc tcagccaggc gtagttcgag acgagcgaac accgcgccac
      361 cgagcgcctc gcgcgacgtc agtcgagcgg gttgagcacc atgcccgtcg cgagcttcgg
      421 gtagaagtac gtcgacttct gcgggagcac ctcgcccgcg tcggcgacgg cgacgatctg
      481 ccgcacgtgc acgtggtcga cgaggaaggc cgctccgtac gcgccggcgt cgacttcgcg
      541 ccacgcctcg gtttcgtcct cggtgtatcc aaccgcgccg ctcgcccgct gcgccgcgtc
      601 gaggccgagc agcggctgga gcacagtctc gttcaggatg aggacatgca gcgcgcgcga
      661 ggcagctgac caggcacccg gcatcgcggc ctcgatcgcc tcggcggagc gggctgtcag
      721 cacgtggaac gcctggggct cgaggccgat cgcgccgaag gacactcgcg agccgacttc
      781 cctctgaacg cgggcccaca gactcgcgac accctcggca ctccatggga ggtcgaggcg
      841 ctccacgtca tagagatcgg tgagccgcgc cagcaggtcg gtgggcaccg actcgcgacg
      901 gatgagccgg tgaatcggca gcacgacgag gccggggtcg tcgacgggga cgaggcccgc
      961 gaggatgtag cggtctgcct cggacgcgtc tccggcagct tctcgttcgt ggaggtagtt
     1021 cagcgccgtg tggtagcggt ggtggccgtc ggcgatcgtg acgtttgaga cggcgacggt
     1081 tgtcaccagc gagtcgatgt cctcggccgc ggtcacgggc cagaggcgat gcgattcgcc
     1141 gcgcgcatcg acggcgacga acgccggctc cgcggtggca atgcggtcga ggacggcgcg
     1201 agcgcggccc gcggcgtcct cgaacatccc aaagatgggg ctgacgttgg tgcgggtcgc
     1261 acggaggaga cgcaaacggt cctccttggc ggcggtgagc gtcgcctcgt ggggacgcac
     1321 gataccgtcc tcgtgcgggt ggattcgcag ccgcgcgaag aaggaacggc ggacgtacga
     1381 gccgccgtgc acctcaaatg cctgctcgta gacgtacagc gccggccgct cgtcgcggcg
     1441 cagcgcgccc tcgcgctccc agcgttcgag gagcgccgca gcctgggcgt acccggcgtc
     1501 agacgcttc
//
